LOCUS       DAORQT010000001        30293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_72_length_30293_cov_14.386963, whole genome shotgun sequence.
ACCESSION   DAORQT010000001 DAORQT010000000
VERSION     DAORQT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 30293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30293
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_30293_cov_14.386963"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1050)
                     /locus_tag="PK134_00005"
     CDS             complement(97..1050)
                     /locus_tag="PK134_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRB37265.1"
                     /translation="MRTAILIATIFTTLNISCATGQKNSSSNEPDMKEQLKNGTNIYF
                     ENKTISTDLNFTNLLQPVVESNGINRCYVNSAITFKQCTFKGKITGYATDQSGKSTMT
                     YFQKSVTFIDCIFEDDINLRGAIFSAPANFQGCSFVKKADFQECHFYTDALLNETKYR
                     EEAFFQNAVFMKKMILMNARFESNVYFQNAVFYFDAQFSNAEFQKYADFTVCTFQMGV
                     FFNYCKFQQQAVFSSSVFSKRFEILSTTAQQLDMRKCHFGFVPKLMKSNIVSELDFSG
                     SHFTDGIPDLKETSAATILASEIVLCDKSYSQEQFLKASGK"
     gene            complement(1153..2865)
                     /locus_tag="PK134_00010"
     CDS             complement(1153..2865)
                     /locus_tag="PK134_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRB37266.1"
                     /translation="MKWIKRLFLVTLALSLTISACKKEKDDSPSPTPPTPIPPTPSGN
                     TPKNLGWNGGDNPNSVPTSVTNTPVFNSGTLPTSVSLLPQFPPIGDQGQYGTCVAWAA
                     GYNMKTVIDAIQQNLNASDLTNTNKQTSPKDVFLAIPDAKKGQNCSGTNFSDAMDLLL
                     NRGAATMQTVPYTNLGNCANSGVQSNWTQEANQRKIKNYRKVSPNIQSIKEQLANKIP
                     VVIGAKLSDNFMTWNSDAVYNSNSTYNQVGQHSYHAIIISGYDDSKGANGAFKVVNSW
                     GTNWGDVGYIWIDYNFLVNEFVFDQNFYTATTDGGTNPPSPTPTPSGGVDLASWVFGD
                     ETTYGTSQTQYYNERVVYWNLYNIGTSGAASSSNWSLYYIYFNAYDANDYGVLFYDEF
                     NTSIAQNTYQVDPNDPNHIILNIDIPANNDLGNVGFGTQQLYQVYYTPSTLNGEYYIA
                     MIADAENAFTEDNEQNNFFYTTDQYPKTFQNGYSGRITAQMQSTNATNYVEYKFINKL
                     QPSKHNLTSSIYNTVVTKQRPNAYSSEEIGQMLKWKKKTGDLDRKLAEFKMRQKGNGG
                     FGAK"
     gene            3020..4207
                     /locus_tag="PK134_00015"
     CDS             3020..4207
                     /locus_tag="PK134_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRB37267.1"
                     /translation="MNIDKIKQLAEIAFNIVLADRRHLHKNPELSFKEFQTQQYVWER
                     LDEIGISNKKKIAETGIIAMIEGKNPSAKIIALRADMDALPIQETNKTDYVSQNAGVM
                     HACGHDVHTSSLLGVASVLQQLKSEFSGTVKLLFQPGEEKLPGGASLMIKEGALKNPS
                     VSGILGQHVMPMIETGKVGFRSGVYMASTDEIYVTVKGKGGHGAIPHQNIDPVLIASH
                     IIVALQQIVSRNANPIMPSVLSFGKVMANGATNVIPDSVYLEGTFRTLDEKWRSDAHT
                     KMIRMANAIAEGMGGSCEFKIIRGYPALVNNPQLTASARKNAEAYLGKENVLDLDIWM
                     AAEDFAFYSQETNACFYRLGTRNEANGITSSVHTSTFDIDEKALLTGVGLMSYLALCE
                     LEQ"
     gene            complement(4249..7116)
                     /locus_tag="PK134_00020"
     CDS             complement(4249..7116)
                     /locus_tag="PK134_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRB37268.1"
                     /translation="MKNNLYSKLGCLLLLAPAFSFAQLSFTNSNSRLASPAFHSGCPV
                     SVVDWNNDGLDDIIRLDNGKHCYVDVHKTNQQYQRIFLGTFSGSTWAMAVADLDKNGY
                     KDVVAGGAFGSVEILKTNNSGTGATLSSLSNSNFFLQNLTMADFDNDGWIDIFCCDDN
                     AASHIYLNNGSGGFGAQSNIINFNLPNGSDNSGNYGSVWTDFDNDGDLDLYIAKCRQS
                     VNNPSDPRRINVLFVNNGNGTFTENAAAYNINIGWQTWTAAFGDIDNDGDLDLMITNH
                     DHNSQIFQNDGTGVYTDITSSTGFTVSDITPIESVMEDFDNDGFVDIFVTGSDSRFWR
                     NNGNSTFTLVENLFDNNDMESFAIGDANHDGWIDVMASYANIYTIPSNIDDVLWINNG
                     SKQNIIDGVAGLPTNNFFNVVLEGNTTNKDAIGARALIYGTWGVQLREVRAGESYGTN
                     NSAILHFGLGTATAIDSMKVIWPSGVSQTISNPSINQFLSIKENDCVSPEAIVTASGP
                     LVLCTSGQALTLTAPAGFNYLWSDSSTAQSITITQTGEYNVTISQPGNNCSATSATMV
                     VIPSPNQTPAITALGETVFCNGGSVQIQGPIGFSSYLWSNGDTTQTATVTTNGPVTLT
                     IQGICQSFTSDPINVTVNTVTDPIANDVILSAAGSATLNATVGNNISWYATNAGGTPL
                     ATGPSYTTPVLTANTTYYMQATDTFGGGIYAVGLPTWSGSGSGYSGNTTNATTEFNVT
                     KNCTLKSVKVFTNVAGIRRIELKDASSTVINYVDVNVSPDSQVVTLNFPLTPGMGYTL
                     GTNTTTNQNNLGTDSPKFKRNNSGVNYPYTLSDALSITNSSNGGQYLYYFYDWQVEKE
                     SWECVSNMVPVNVYITTGIKENDRSILSAYPNPVSDKLNISNPFGTVKATLFDATSRV
                     VGSYVLQNGENIFNIESLNAGVYQLQVNHDGEISNIRLVKN"
     gene            complement(7176..9107)
                     /locus_tag="PK134_00025"
     CDS             complement(7176..9107)
                     /locus_tag="PK134_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37269.1"
                     /translation="MKNKIIVLFLISVAIIGISAIPFQPKKAMAYHSESEIAFFKNHS
                     MTPIAPGEFFLTPDNCKGCHGFDTLGIANVDANGQDVNLHDDWETSMMGLSAVDPFWR
                     AKVSHETMVNPAHATELQNLCITCHAPAGHYSAIYKGQQHYVMSDLLNDSLGLSGVSC
                     TACHAIGDSSSLGQLFTGQIPYDTNKVLFGPFISPVAGPMQLYVGMIPTYSPHVSEGK
                     FCSPCHTLLSNTVDLNGIPTGGTFIEQATYHEWLNSTYPSQATVCQSCHMPQIEDAVI
                     LANGNVGLPGRTPFNLHQFAGANSFMVNLIKQNKTALGVSAPDYNFDSTLAAITHMLT
                     TQSLDVQASLAAITSDTAFIDVDLTNKAGHKFPSGYPSRRAVLQMIVTKANGDTLFAS
                     GLFDANYEMQNVASPFEQHHDIINSQTQNQIYEMVMGDVNGTVTTVLERAASHLKDNR
                     IPPAGFSTLHYAYDTCKIVGSALTDVDFNKNGATQGTGHDIVHYHIPLNNYSGTINVY
                     TAVYYQTLPPAFLSDMSNYSSPEINTFLTMYANADKSPVKVDADTLLNISTGIKENIL
                     TTINISPNPTRDGKVLLSGIGYNSFQLEVFDQNGKKINVILTRKNNSIEVQLPTAKGI
                     YFIRLNVNGQVKSYKILRI"
     gene            complement(9270..11738)
                     /locus_tag="PK134_00030"
     CDS             complement(9270..11738)
                     /locus_tag="PK134_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRB37270.1"
                     /translation="MNKYLRYFVFLLLTCLALLLIAMGLSVLLQDKIKQSVIEEINKS
                     LNTPASVSEIDFSVLRFFPNASLTLKNVVIKGNPWQDTKRPLLKAGKIDLVLSITSIF
                     SSTIQLKKIEISDAELNILTNENGQKNDQIFKQGNDNNSFAAEFKKVILKNVNLSIEN
                     KPSNFILQTHLNKSLASGNFKDKTFELKTETDLFAEIIQHENISYIKQKPIQLNGVVS
                     VDLRNDIYAFNDLKIQLANLNINAKGKIEKRNETFLDLKFTATKANASELLSILPSNW
                     VAPQILNYRYHGNINFDTHINGVASSKQSPTIEINFGTNKTDIIPDNENYALKNVALK
                     GFYSNRKSGKNISVLSLKNISARIEGKPIIGEVYLENLKNPYFNIQLDADISLASLSR
                     YLPADFTEEQQGSVSLHGRISGQADKKDTYRSNGTLTLNNLKFKLKKRNIAVNSISGK
                     INFNNADVKVESLKILAGQSDVVINANILNFYNYIFRDNQTITINGIVESHFLDAGEL
                     LAGNQSTDTSDFDLPAYLNLNASLSADVVKFRKFNAKDLSGNISLQDKILTAKQLSFN
                     AMEGHIALDGTINTNFKDEVHISSNAKIESINIERLFFETGSFGQQVLTEKNIRGTLT
                     ASVQLATYWTKQLEVKQSRTIASADLSINDGALIDFKPMLKLSRFVKGSDLQNVRFSK
                     LQNTIRIHEQKILIPTMDIRSTAMNMTISGTHGFDNFVDYKIQMKLSQLLGKKVRQQN
                     TEFGTIEDDGSGGLNLFLTMKGPLDNPKFSYDRKGVEKKITESVKSGKDDFLKIIKEE
                     ISGKKPPKEETPKKQKELQIDYDE"
     gene            complement(11932..12534)
                     /locus_tag="PK134_00035"
     CDS             complement(11932..12534)
                     /locus_tag="PK134_00035"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRB37271.1"
                     /translation="MSFQLPPLPYAPDALEPHIDTRTMEIHHDKHHQAYVTNLNNAIA
                     GTDAEKMSIEDICKNISKLPMAVRNNGGGHYNHSLFWTIMAPNAGGAPKGKLADAINS
                     AFGSFDEFKTKFNQAAATRFGSGWAWLIKNAAGKLEVCSTPNQDNPLMDVAEVKGTPL
                     LGIDVWEHAYYLHYQNRRPDYCTAFWNVVNWDEVSNRFEK"
     gene            12763..13035
                     /locus_tag="PK134_00040"
     CDS             12763..13035
                     /locus_tag="PK134_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37272.1"
                     /translation="MKKVLALVMAAGMFSIVACGPSAEEKQKMEETAKAAQDSIAAAA
                     QQAASAAEQAATDAAQVATDAAQTVDSTAGAVVNQATEAVKEAATK"
     gene            complement(13086..14210)
                     /gene="dprA"
                     /locus_tag="PK134_00045"
     CDS             complement(13086..14210)
                     /gene="dprA"
                     /locus_tag="PK134_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRB37273.1"
                     /translation="MENNLTTDVLQYQLALTLLPGVGDITAKNLVGYCGGAEAIFKEK
                     QQALLKIPNVGQIVADKIKNGKEQSLKRAEEEIRFIEKNKIKPLFFTSDQYPKRLKAC
                     NDSPIMIFVKGNGNLNPEKCIAFVGTRKASDYGKTTTEKLIADLAETDGMMIVSGLAF
                     GIDITAHKASLKCKIPTAGIVAHGLDTLYPAEHRNTAEKMQAEGAVISEFMSKTNADK
                     ENFPKRNRIIAGMADAVVVVESGIKGGSLITADIANSYNRDVFAIPGQVDKEYSKGCN
                     ALIRDNKANLIQSAADIEYFMGWDKTDVKSIPKQARLFEDLNPEEKQLVDFLKENSKV
                     DIDTICFRSGVKLNRIPALLLNLELKGVIKNYPGKVYDLI"
     gene            14338..16548
                     /locus_tag="PK134_00050"
     CDS             14338..16548
                     /locus_tag="PK134_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB37274.1"
                     /translation="MNAQQQRVSGKVTDGEGELPGVAVYADESHATTSDAKGEFVLML
                     DKGSWTLKAKLLGYETFSKVILVGDSNYVQIHLIPLSKQLQSVVVSAGKFEQKLEEVT
                     VTMETIKPKSFETTGDNNVETALEHVTGLNVNDGQANIRGGSGFSYGAGSRVLVLVDD
                     MPMLSADANDAKWSFLPIENLEQIEIIKGASSALYGSSAMNGVINFRTGYAKSEPVTK
                     IVQYNGIYDSPRRKEIKWWGKETQMLSGANMFHAQKFGQWDFVAGGHYLLDEGFRKGE
                     TEKRARVNINTRYRFPKIEGLAVGVNANYMESFGGLFIIWNDDTTGAYIPSGGVNDST
                     STLSNYHTVRYNIDPFITYHSKKGFSAKWRSRYFRTLNYNDTDQEAFADLYYSEVQLQ
                     QRFEKWNLVLTGGGVITDGGVRSDLYGSRKTYNKALYAQADKKFFDRLNLSLGWRWEQ
                     FRFRYSKAESKPVIRAGANLRLLRATYLRSSYGQGYRFPSIAEKYVKTRVGSIVIYPN
                     DSIFSESGWSSEIGLRQLFAVGKQFSGYVDASVFRSEYKNMLEFAFGSYGDPFKDPLF
                     GLGFKSLNIGNTRIDGMEFELGVQATVLKKINLQFGGGYTYIDPRLTDFDSLKASTGT
                     ITYNFLKYRYRNLFKGYGSIAYGKVSLAVNCRYNSFMENIDVVFNELLPGVKRYRAEH
                     DKGDWIYDANISYKLNSQIALSVISRNLFNHEYSGIPSQLEAPRMWLFQFVITP"
     gene            complement(16594..17262)
                     /locus_tag="PK134_00055"
     CDS             complement(16594..17262)
                     /locus_tag="PK134_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB37275.1"
                     /translation="MNKTIPDKPLRVIIVDDNKNMRESVALLLQSTPSLLLIAEFDSG
                     EECIKQVDTLKPDVILMDIDMPKLSGIETVKIIRKQFPGLPILMLTGFEDDDKVFDSI
                     CAGANGYILKNTSMELLIHHIHEVYTGGAPMTPVIARKVLTRFAKTQVTETKNEDYNL
                     SKREMEVLELLVKGKSYKTIATELKVSYETVHTHIRKIYHKLHVKSIGEAVSKTIKNK
                     ILNS"
     gene            complement(17311..20391)
                     /locus_tag="PK134_00060"
     CDS             complement(17311..20391)
                     /locus_tag="PK134_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRB37276.1"
                     /translation="MIKFFKSLLLFLVFTLPALADSLTINLNEFTANDGLSDNQTNCV
                     IQDKYGFIWIGTKDGLNRFDGKSFQVFKFQSDKNSVSGNNITCLEVTDDSLLWIGTAS
                     NGFCSYNYKTNTFQNYHARNSPLRAEYINDLAFDKVRNCIWVAMNNGGLQQFDLKNKK
                     LNPYVYSINSYYAVAVKDTISLFAGIIESLKKLDNVGRNKGMVGDTATTINTIYIDND
                     LNVWCGAWDNALHLFNIHGKRLQSYIFDGTSKFKKSGEEIISISSDASNNIWCGTKSS
                     GIFIFNKASKSFINNINFSHQINTRVNELFRDSQNRIWIATENGLLVYDQHRNQFRTE
                     MLPVPKGVNACKVFDRLITDKGLDLVITQCGLFYKKSPNESYKFKTVIYRNEEQELYS
                     ILMTKNGDIYIGSNRTVFKFNPENISFNTIDFNKNLKETSFYFNGASLVNSLTSFKQD
                     NSELIAASYYGNYIVLIDFDNRNLYYLIHENTKISDNLENLSRKIWVDSKNNFWVCGR
                     SKGLVQLKFKSDFSLSKYAADNDSMLPITVMPTNWNMLPNSIPIKIKNVFDIAERKDG
                     TYWLSTQGAGLVLFSKENKTSPFKIIDSDITSMQGIKSTGDSLLWIITSSGLLQYNEK
                     TNFFKLYDASSGLSQNIFGYFFQNKPGDNNTLSVGFNGGFISYNPSQLLSSTEKPNLQ
                     FTKLWVMDKPSDSLLFSDIKLHHNQNFLKVYVSPNIYTNSDKITYFYRLSGIDDNWRS
                     NDNNPIITYTNLPPGKFRLDVKAISNNNLQSDIKTLSFVIVPPFTSTIWFYGIVVFSI
                     GAFVYFIYRYRINQLLRLQEVRNNIARDLHDDIGSTLGSIHLYSQIADNKMKQDKHHE
                     IPGILEKIKKSSKEIIEKTGDTVWVVKASNDNMSNLSLRMETYAASILGDADINFNFI
                     VEENIQNLKINMAQRKNLFLIFKEAIHNIVKYSHCTQVDIHLSRNRGMTFLKIKDNGC
                     GIDMANNHSQTNGNGGNGLKNMASRAKDLNAEFQINSAPGKGTEITISLKQHAI"
     gene            complement(20540..23743)
                     /locus_tag="PK134_00065"
     CDS             complement(20540..23743)
                     /locus_tag="PK134_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37277.1"
                     /translation="MKRVANLFISLAFLLYVKAASAQLTLAEWNFPNNPDNATVDISI
                     PANSAKTIYTVGGTAAVVYTTAGATTQSAWTTGWDNGSGTKYWETEINTTGYFNLELS
                     SKQRSSPTGPKNFAIEYKIGIAGTWTPIPGAGAIVVADNFTSGVVTDIPLPVACENQA
                     SVFLRWIMTTNTSVNNSTVASGGSSRIDDVIIKANDAQNHYRSITSGAWNSLSTWQQS
                     PDLVTWTAAVFLPNYYAKTITIRSGHTVTISANVRIDETTINNGGTVNYNSKLLTINN
                     GTGVDLQVNGTFTDGSSTSVAWLAGATWALGASGTYVKTENTNATNWQNNYQGGISTI
                     PATASWVLNKTPSSVTAPSLTTLNMFYPNLTIMNTSGGVWTTAVSSTFTGSTATATIK
                     GNFDVGGVLAPLLGTVDFLNDNNFATPVQVQGNILIRGGCNLRNFGTGFEIFGNLTVN
                     GSTSYGAANARKYRFSGGNAQSISGSAALTSLQIYQLEMNKSANSLTLLKSVKVDNNL
                     NLITGIINSSTPNILIVENGATATGASNVSFVNGPVQKNGSQAFTFPVGKNTSYRSIA
                     IGADASSSSFWTENFNTGAGWSLVNVMGPEGADPNPFVISDAEGGVAAGGCGVANNGN
                     NTMHVSSVFNPTGGAAYDAGGLCGLLYCPQTNRMAQSPVINCTGKSNISISFNYIEFG
                     DGTIDNATLWYYDGAAWAQITDMPKTACCGGACNGFRQGKWTAFSMVLPASANNNANV
                     RIGLKWENNDDGVGTDPSFAIDDISLSSLTSSQFVAEYMRANPQITYSNIFDPTLDHI
                     SQCEFWTLNQIAGSSSRNVTLSWDASSCGVTLLSDLRVARWRSVGTIWNNLGNGSTTG
                     NVSAGTITTSTADNLFGPFTLSSASSENPLPVELLNFTAQLNNDQVELRWSTVSEINS
                     DYFEIERAAVDLNFIPINKTQAAGFSNQLLRYHATDSKPLARKSFYRLRQFDFNGESH
                     LSNSVAVELNNYEQLQINYAVFTNKNNLYFKVTGNVEKNLTAEVYDINGAIISTQELN
                     GTENIFHVQTQNLANGIYVLKISNSNSSVTKRFSVHD"
     gene            complement(24006..24314)
                     /locus_tag="PK134_00070"
     CDS             complement(24006..24314)
                     /locus_tag="PK134_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37278.1"
                     /translation="MKLKKGKRKFAFDFVHPAFKIESNSTLLNIFPNPSSGVFNVQSD
                     NQLENVQITVTDLQGRTLINTLFSGNETTVDLSNYTQGVYLLQLHSSNVVQTYKLIKD
                     "
     gene            complement(24771..26522)
                     /locus_tag="PK134_00075"
     CDS             complement(24771..26522)
                     /locus_tag="PK134_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRB37279.1"
                     /translation="MKKTLLTIFVMATTLLVQAQTPAFEWVQQVGDSAGDQGRSIARD
                     AAGNLYVAGCFGKTVDFDPGPGVYNLTSSVGLELFVLKLDTAGNFEWAVKTTGWIGNL
                     SEVALTCSDAGNVFVTGHFKDSVDFDPGSATFNLTSNGGRDIFIWHLNTNGNFVWAKN
                     IGSTASSINSRDVGYDITTDNWGNVYTIGSFELSADFDPGPGVVTLNAGPYCDIFISK
                     LNATGDFVWAKNFGGFNYCIGNSIAIDALGNVHATGSFSDTVDFDPGAGVFNLIAPRS
                     NQFVASDPDIFILKLDSTGNFIWAQNFGATSEDVGQAISVDANGNVFTTGYFEDAVDF
                     EWGNGTFNLTTSIFEDVFVLKSDAAGNFLWAKQIVSSSFYDAGNGIATDVAGNVYITG
                     SFAGTADLDPGIDSAFVTAASSSPDIYILKLDAGGNFLWGGRAGDIGNDAGEEIVTDA
                     SGNIFTAGSFSRTADFDFNTGVNNLTTVNTLSNVYVLKMNQNNVGIYENMIQNKFTVY
                     PNPATNILKINNLKFEIGSAIKIFDVMGKEVLHTVVGGAETAINVSKLESGIYFVHLF
                     SVSAPEENILTEKIIIH"
     gene            complement(26534..28267)
                     /locus_tag="PK134_00080"
     CDS             complement(26534..28267)
                     /locus_tag="PK134_00080"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37280.1"
                     /translation="MKKTLLTLCCFSALTLNAQTPTFDWAINIGSTNFDKGYDIATDA
                     AGNVYEIGILAGTADFDPGAGVVNLASNGNDDIFIAKYSTTGNLLWAHNIGGTGNETA
                     VAMALDASGNCYFTGHFTGAVDFDPGAGTSIITASGAFDVCIVKLNSSGNLVWAKNFG
                     GTYGTFVDELSLDASGNVYTEGTFQTTTDFDPSAGVYNLVSTGGQDPYLAKLDAAGNF
                     VWAINTGFSVNSLHIDNNNTVYLAGSFNDTTDFNPNATQVFNVIPIGATDIYIAKFDT
                     AANFIWVKNIGGTTGTDGGNAYAINTDAAGNIIVTGTFTGTVDFDPGTGTANLTSVGT
                     GTNMSDMYFLKLDASANFVWVKRVGGNSIDVPLDIATDNAGSVYFSGRFKSSIVDFDP
                     DPTVTFNLNGTFSSYDAFVLILSATGNFVYAYEIGGPSNGDDEANAICVNPTGSFYVT
                     GTFYGPIDFDPGTGTTTLNDIGNTDAFIQKMNFSTVGLAHLTDENYFILYPNPANQLL
                     VVNHGLLKENCELKIFDVTGKEVHTSTISGNQSTIDVGKLENGIYFLQLSYSSPSMKS
                     SGASQKLIIHH"
     gene            complement(28369..29589)
                     /locus_tag="PK134_00085"
     CDS             complement(28369..29589)
                     /locus_tag="PK134_00085"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRB37281.1"
                     /translation="MRNLDTLKLIFTLVIVGSLSMNSIKAQTWTQQTSNTTEDLNSVH
                     FFDANVGGAAGFNGTILTTTNGGTTWTNQTSGVTVNLFDIFFADSQTAYSVGLGGTIL
                     KSTNGGTTWVSQTSGTTQLLLDVFFVNPLEGWVVGNSGTILHTINGGTTWTPQSGMNN
                     AFKAVYFISPTEGWAVGYPLILHTTNAGTTWSQQTAPVVHYNDVHFINATEGWVVGEN
                     SIGLKTMDGGITWNSSSFSFQTSIIAVHYVSSSVGYINLEASLPSNNTLRKTTDGGVT
                     YQVEPTGVNARTTDSYFINAGLGWVVGYDGVILKYNSGTGIDNINDNSNINIYPNPAT
                     NKFEVKGLMFDGAYELRIFDVMGKEVLKTNVIGEKSIIDVTNIKNGIYYVQISNSSSL
                     GKSAGAYQKLVIQH"
     gene            complement(29791..>30293)
                     /locus_tag="PK134_00090"
     CDS             complement(29791..>30293)
                     /locus_tag="PK134_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37282.1"
                     /translation="CANTQGIQFDADMSNTTGAYTLNWTFPNAPTASYVSGGGNSNSL
                     TLDWGTGNGTVIIKASNSCGEGSKAYVANIGCKEAQQVDAGSIAAYPNPTAGMLNVEF
                     NAVEKGNASVSVIDLSGRTILNSSQDVSAGNNTMQLDLTKVAKGAYMISVKSNQFAKQ
                     IKIVVE"
BASE COUNT         9347 a         5388 c         5585 g         9973 t
ORIGIN      
        1 gtaacggtat attactgcaa aacggatttt gaataaatct tacttgctta aagtagctaa
       61 aatgttctat tttcaataat gaaatcaaaa ataagcttac ttacctgagg cttttaaaaa
      121 ctgctcctga gagtaacttt tatcgcataa aacaatctct gatgctaata ttgttgcagc
      181 agaagtttct tttaagtcag gaatgccgtc tgtaaaatgg cttccgctga agtcaagttc
      241 tgaaacaata ttacttttca tcaatttggg gacaaaacca aaatgacatt ttctcatgtc
      301 caattgctga gctgtggtac taagtatttc gaagcgttta ctaaacacac tactactgaa
      361 aactgcctgt tgttgaaatt tgcagtagtt gaagaaaaca cccatctgaa aagtgcaaac
      421 agtaaaatca gcatatttct gaaactctgc attgctaaac tgcgcatcaa aataaaaaac
      481 agcattttga aaatatacat ttgattcaaa tcgtgcattc ataagaatca tttttttcat
      541 aaatacagca ttttgaaaga atgcttcttc gcgatatttg gtttcattta ataaagcatc
      601 tgtataaaaa tgacattcct gaaaatcggc tttttttaca aagctacaac cttgaaaatt
      661 tgccggagca gaaaatattg ctcctcttaa atttatatca tcttcaaaaa tacaatcaat
      721 gaatgttaca cttttttgaa aataagtcat tgttgacttg cctgattgat cagtagcata
      781 tcctgtaatt tttcctttga atgtacactg tttaaaagta attgcagagt tgacataaca
      841 tcggtttatg ccattgcttt caacaacagg ttgcagcaga tttgtaaaat ttaaatcagt
      901 actgatagtt ttattttcaa aataaatatt agttccgttt tttagttgtt ctttcatatc
      961 cggttcgttt gaagagctgt ttttttgtcc tgtagcacag gaaatattta gtgttgtaaa
     1021 aattgtagct ataagaattg ctgtacgcat aatatttttt ttacaagttt aaaaatcatt
     1081 tggtgaaaca gaaaaaatcc cggcagaaat tttcttaccg ggatgatatt gtttgcggtt
     1141 taaatgttga atttatttgg caccaaagcc tccgttgcct ttttgacgca ttttaaattc
     1201 tgctaattta cggtcaaggt caccggtttt tttcttccat ttaagcatct gtccaatttc
     1261 ttcagatgaa taagcatttg gacgttgttt ggtaacaaca gtattataga tgcttgaagt
     1321 taaattgtgc tttgatggtt gaagtttatt aatgaatttg tactctacat agtttgttgc
     1381 attggttgat tgcatttgcg cagtaatacg cccactgtag ccattttgaa atgtttttgg
     1441 atactgatca gttgtgtaga agaagttatt ttgttcatta tcttctgtaa atgcattttc
     1501 tgcatcagca atcattgcaa tataatattc tccattcaga gttgaagggg tgtagtaaac
     1561 ctgatacaat tgttgtgttc caaaaccaac attaccaaga tcgttattag caggtatgtc
     1621 aatattcaga ataatgtgat tgggatcatt cgggtcaacc tgataagtat tttgagcaat
     1681 agatgtattg aactcatcat agaaaagcac accataatca ttggcatcat aagcattaaa
     1741 gtaaatataa tacaaactcc agtttgaaga acttgctgct cccgaagttc caatgttgta
     1801 tagattccaa taaacaacac gttcgttgta atattgtgtt tgtgatgtac cataagttgt
     1861 ttcatcacca aacacccaag aggctaggtc aacgcctcct gaaggtgttg gagtaggtga
     1921 aggaggattt gttcctccat cggttgtggc ggtataaaag ttttgatcga atacgaattc
     1981 attcacaaga aaattataat ctatccagat atatccaaca tcgccccaat tggttcccca
     2041 tgagtttaca actttgaatg caccattggc acctttacta tcatcataac cggaaatgat
     2101 aattgcatga taggaatgtt gtccaacctg attgtaagtt gaatttgaat tgtaaacggc
     2161 atcgctgttc caagtcatga aattgtcgga aagcttagct ccaatcacta ccggaatttt
     2221 attggctaat tgctctttaa tactttgaat attcggactg acttttctgt aatttttaat
     2281 cttgcgttga ttcgcttcct gtgtccagtt actttgcaca ccggagttgg cacaattgcc
     2341 tagattggta tatggaactg tttgcatagt agctgcgcca cgatttaata ataaatccat
     2401 tgcatcagaa aagtttgtac cactgcaatt ctgacctttt tttgcatccg gtatagcaag
     2461 aaaaacatcc ttaggactcg tttgcttgtt tgtattcgtc aggtcgcttg cattaagatt
     2521 ttgctggatt gcatcaataa cagttttcat attgtaaccc gcagcccatg ctacacaggt
     2581 tccatactga ccctgatcac caattggagg aaactgtggt aacaaactaa ctgatgttgg
     2641 taatgttcca ctattaaata caggggtatt ggtaaccgag gtaggcacag aattggggtt
     2701 atcaccaccg ttccatccta aattttttgg cgtattcccc gatggtgttg gaggaatagg
     2761 agtaggaggc gtaggtgaag ggctatcatc tttttctttc ttgcaagcac taattgtaag
     2821 tgatagtgcc aatgttacta agaataatct tttaatccat ttcatttttt aaaattttaa
     2881 attgatgagt gctacaaagt agaatataac aaactggatt gtcaatcacc aaatctggtg
     2941 atttttatag ctgaaaacaa tacttaattt gatgtataaa aacgtaaaat attgaacaat
     3001 aaagttttaa ttttgacaca tgaatattga taaaataaag caacttgcag aaatagcgtt
     3061 taacatagtg cttgcagaca gacggcattt gcataaaaat cctgaacttt catttaagga
     3121 atttcagaca caacagtatg tttgggagag attggatgaa ataggaataa gtaataaaaa
     3181 gaaaatagcg gaaaccggaa ttattgcaat gattgaaggt aaaaatcctt cggcaaaaat
     3241 tattgcactg cgtgctgata tggatgcgct tcccattcag gaaaccaata aaacagatta
     3301 tgtttcacaa aatgcaggtg tcatgcatgc atgtggacat gatgtgcata cttctagtct
     3361 tctgggggtt gcttctgttt tgcaacaatt aaagagtgaa ttttcaggaa ctgtaaaatt
     3421 attgtttcag cccggagaag aaaaactccc cggtggtgct tcattaatga taaaagaagg
     3481 ggcattaaaa aatccttcgg tttcaggaat tttaggacaa catgtaatgc caatgattga
     3541 aaccggaaaa gtaggtttca gaagtggtgt ttatatggca agtacagacg agatttatgt
     3601 aactgtaaaa ggaaagggtg gacatggagc tattccgcat cagaatattg atccggtgct
     3661 catagcatca catataattg ttgcgttaca acagattgta agtagaaatg caaaccccat
     3721 tatgccaagt gttttgtcgt ttggaaaagt aatggccaac ggagcaacca atgttattcc
     3781 tgacagtgtt tatcttgaag gaactttcag aacacttgat gaaaaatggc gaagtgatgc
     3841 acatacaaaa atgattcgta tggcaaatgc tattgcagaa ggtatgggtg gaagttgtga
     3901 attcaaaatt attcgaggtt atcctgcatt agtaaacaat ccgcaactga cggcatcagc
     3961 aagaaaaaat gccgaagctt atttggggaa ggagaatgtt ttagatcttg acatctggat
     4021 ggcagcagaa gattttgcat tttattcgca ggaaaccaat gcttgctttt accgtttagg
     4081 gacaagaaat gaagccaatg gaattacttc atcagtgcat acttcaacat ttgatattga
     4141 tgagaaagca ttgttgacag gtgtaggatt gatgtcatac ttggcacttt gcgaattaga
     4201 acaataaaaa aaccgcccct tttcagaggc ggctgctaag ttttttgttt agtttttaac
     4261 taagcgtata ttacttattt caccatcatg atttacctga agttggtaaa cacctgcatt
     4321 gagtgattca atattaaaaa tattttctcc attttgtaaa acataactac ctactacacg
     4381 cgatgtagca tcaaacaatg ttgcctttac agttccgaat ggattagaaa tatttaattt
     4441 atcactaaca ggatttggat aagcagataa aatacttctg tcattttctt ttataccggt
     4501 ggtgatatac acattcacag gaaccatatt gctaacacac tcccacgatt ctttttctac
     4561 ctgccagtca tagaagtaat ataagtactg cccaccatta cttgagtttg taatgcttag
     4621 cgcatcagat aaagtatatg gatagtttac accggagttg tttcttttga atttaggtga
     4681 atcagttcca agattattct gattggttgt ggtatttgtg ccaagtgtat aacccattcc
     4741 cggagttaac gggaagttta gtgttactac ctgcgaatca ggagaaacgt ttacatcaac
     4801 ataattgata acagtggatg aggcatcttt taattcaatt cttctgattc cggcaacatt
     4861 ggtaaatact ttgacagatt ttaaagtaca gttttttgta acattaaatt ctgttgtagc
     4921 attggtagta tttccagaat accctgaacc agaacctgac catgttggta atcctactgc
     4981 gtaaatgcca ccaccgaagg tgtcagttgc ctgcatatag tatgttgtgt tcgcggtaag
     5041 aacaggtgta gtgtaacttg gtcctgttgc taaaggtgta cctcctgcat tggttgcata
     5101 ccatgaaatg ttattaccca ctgtagcatt taatgtagca cttccggctg cagataaaat
     5161 aacatcgttt gctatcggat cagtaactgt atttacagta acattaattg gatcagaggt
     5221 aaatgactga caaatgccct gaatagtcaa tgttacaggc ccattagttg ttacagtagc
     5281 tgtttgtgtt gtatcgccat ttgaccaaag gtagctgctg aatcctatag gtccctgaat
     5341 ctgaacacta cctccattgc aaaacacagt ttcgccaagc gcagtaatag caggtgtctg
     5401 atttggtgat ggaataacaa ccattgtagc tgatgtagca gaacaattgt ttccgggttg
     5461 tgagatagtt acattgtatt cacctgtttg ggtgatagta atactttgtg cagttgaact
     5521 gtcgctccat aagtaattaa atcctgcagg tgcagttaaa gttaatgcct ggccacttgt
     5581 acacaatact aaaggaccag atgcagtaac gattgcctca ggtgaaacac aatcattttc
     5641 tttaattgat aaaaattgat taattgaagg attgcttatt gtttgactta caccacttgg
     5701 ccagattact ttcatagaat caatagccgt ggctgtacct aaaccaaaat gtaaaattgc
     5761 actattattg gttccataac tttcacctgc tcgcacttcg cgcagttgaa ctccccacgt
     5821 tccataaatc agtgcacgcg caccaatagc atctttattg gtagtatttc cttcaagcac
     5881 cacattaaaa aaattgttgg tcggtagtcc ggcaacacca tcaataatat tttgtttact
     5941 accgttgtta atccacaata catcatcaat gtttgaaggg atggtgtaaa tatttgcata
     6001 acttgccata acatcaatcc agccatcatg gtttgcatca ccaatagcaa atgattccat
     6061 gtcattgtta tcaaataaat tttcaactaa agtaaaagtg ctgttaccat tatttctcca
     6121 aaagcgtgaa tcgcttccgg ttacgaaaat gtcaacaaat ccatcattgt caaaatcttc
     6181 cattacactt tcaataggag tgatgtcaga aacagtaaat cctgttgatg atgtaatatc
     6241 agtataaaca cccgttccat cattctgaaa aatctgactg ttgtggtcgt gatttgtaat
     6301 catcaaatca agatcgccat cattgtcaat atcaccgaaa gccgctgtcc aagtttgcca
     6361 gccaatatta atgttgtaag cagcagcatt ttcagtaaaa gtaccattac cattatttac
     6421 aaatagtaca ttaatacgtc tagggtcgga aggattattt acagattgtc tgcatttcgc
     6481 aatgtataaa tcaaggtcgc catcgttatc aaaatcggtc cacacacttc cgtagtttcc
     6541 tgaattatca gaaccattgg gtaggttaaa gttgataata tttgactgtg caccaaatcc
     6601 accggagcca ttattcagat aaatatgact tgctgcatta tcatcgcaac aaaaaatatc
     6661 aatccaacca tcgttgtcaa aatcggccat ggttaaattc tgcaagaaga aatttgaatt
     6721 gcttaatgaa gatagtgtag caccggtacc actattgttt gtttttaata tttcaacact
     6781 tccaaaagcg ccaccggcaa caacatcttt gtaaccattt ttatctaagt cggcaacagc
     6841 cattgcccac gtacttcctg aaaatgtacc aagaaatatg cgttgatatt gctgattggt
     6901 tttatgcaca tcaacatagc aatgtttccc attatctaaa cgaataatgt cgtctaagcc
     6961 atcattattc cagtcaacta cagaaaccgg acagccactg tgaaatgctg gagatgccag
     7021 tcttgagtta ctgttggtaa atgaaagttg tgcaaaggag aatgcaggtg ctaggagcag
     7081 caggcatcct aattttgagt aaagattatt tttcatttta aacaagtttc tgatttaatg
     7141 taatttgttt ttatttataa gagatgttat agtttctaaa ttcgtaatat tttataagat
     7201 ttaacctgac cattcacatt aagtctgata aagtaaatac cttttgctgt gggcagttga
     7261 acttcgattg aattattttt tctggtaaga ataacattaa tttttttacc gttttgatca
     7321 aacacttcta attgaaatga attgtaacct atgcctgaaa gaagaacttt tccgtctctt
     7381 gttggattgg gtgaaatatt aatggttgtt aaaatatttt cttttattcc tgttgagata
     7441 ttaagcaatg tatcagcatc aacttttaca ggacttttat ctgcattggc atacatggta
     7501 agaaatgtat ttatttcagg ggaagaataa ttgctcatgt cacttaaaaa tgcaggtggc
     7561 agtgtttgat aataaacagc agtataaaca ttaatggttc cactgtaatt gtttaatgga
     7621 atatgataat gaacaatgtc atgtcctgtt ccttgtgttg caccattttt attaaaatct
     7681 acatctgtta aagcgctacc aacaatttta caagtgtcat aagcataatg caaggttgaa
     7741 aatccggcag gagggatgcg gttatctttc agatgtgaag ctgcgcgttc aagtacggtg
     7801 gttacggttc cgtttacatc acccattacc atttcataaa tctgattttg tgtttgtgag
     7861 ttgatgatgt catggtgttg ctcaaaaggt gaagccacgt tttgcatttc ataattggca
     7921 tcaaacagtc cggaagcaaa caacgtatcg ccattagctt ttgtaactat catctgtagc
     7981 acagctcttc ttgaaggata tccacttggg aatttatgcc ctgctttgtt tgttaggtca
     8041 acatcaatga atgctgtatc tgaagtaata gctgctaatg aagcctgcac atccaatgat
     8101 tgtgttgtca gcatgtgtgt gatagctgca agtgttgaat caaaattata gtcaggtgct
     8161 gaaacaccca atgcagtttt gttttgctta atcagattta ccataaacga gttagcacct
     8221 gcaaactgat gcagattaaa tggagttcgt ccgggcaaac caacattgcc atttgccagt
     8281 attacagcat cttcaatttg tggcatgtgg caactttgac agactgttgc ctgtgatgga
     8341 taagtggaat taagccattc atgataggtt gcctgctcaa tgaaagtgcc tcctgtagga
     8401 ataccattta aatcaacagt gttggaaagt aatgtgtgac agggtgaaca aaattttcct
     8461 tcacttacat gtgggctgta ggtaggtatc atgcctacat aaagttgcat gggtccggcc
     8521 acaggagaaa taaaaggtcc aaatagaact ttattagtat cataaggaat ctgaccagta
     8581 aatagctgac ctaaactact gctgtcacca atagcatggc aagctgtgca gcttacacct
     8641 gaaagaccaa gagagtcgtt caacaaatcg gacataacat aatgttgttg tcctttataa
     8701 attgcgctat agtgccctgc aggtgcatga caagtaatac ataaattctg taactcagtt
     8761 gcatgtgccg gattaaccat agtttcgtga cttaccttgg cacgccaaaa agggtcaact
     8821 gcagaaagtc ccatcattga ggtttcccag tcatcatgaa ggttaacatc ctgcccgttg
     8881 gcatcaacat tagcaattcc taaagtatca aaaccatgac agcctttgca gttatctgga
     8941 gttaaaaaaa attctccggg ggcaatcggt gtcattgaat gatttttaaa aaacgctatt
     9001 tcagattcag aatgataggc catagccttt tttggttgaa aaggaatagc gctaatacct
     9061 ataatggcta ctgaaatcag aaataaaacg attatcttgt ttttcatgcc tgcattatcc
     9121 tcaatatttt tttacggatt gatggcaata atatcaattt ttttaatgtg ctgcaacaga
     9181 aatctttggc aaaacatcaa attattaacg tcaataggtt tgtaatacaa gtaaagagaa
     9241 tggtcggatg aagaaataag aacagtccat tattcatcat aatcaatctg taactccttt
     9301 tgttttttgg gagtttcttc ttttggtggt ttcttgccac ttatctcttc tttgataatt
     9361 tttaaaaaat catccttgcc ggattttaca gattctgtaa tttttttctc tactccttta
     9421 cggtcatagc tgaatttggg gttatctaat ggccctttca tggtcagaaa aagatttaac
     9481 ccaccactgc cgtcatcttc aatggttcca aattctgtat tttgctgacg cacttttttg
     9541 ccgagtagct gagaaagttt catttgtatt ttatagtcaa caaaattgtc aaagccatgt
     9601 gtgccactta tggtcatgtt cattgcagtg gagcgaatat ccattgtggg gataagtatt
     9661 ttttgttcat gaattcgaat tgtgttttga agtttactga aacgaacatt ttgcagatcg
     9721 ctgcctttta caaaacgcga aagtttaagc attggcttaa aatcaataag agcgccatcg
     9781 ttaatactta ggtctgctga tgcaatggta cgactttgtt ttacttcaag ttgttttgtc
     9841 caataggtag caagttgaac ggaagcagtc aatgtgcctc tgatattttt ttctgtaagc
     9901 acttgctgac caaagcttcc tgtttcaaaa aaaagtcttt caatgtttat tgattcaatt
     9961 ttcgcattgc ttgatatgtg tacttcgtct ttaaaatttg tattaatagt gccatcaaga
    10021 gctatgtgac cttccatagc attaaatgaa agttgttttg cagttagaat cttatcttgc
    10081 agtgatatgt ttccggaaag gtctttagcg ttgaatttcc tgaattttac tacatctgca
    10141 cttaacgatg cattcagatt aagatatgca ggcaaatcaa aatcagaagt gtcggtagat
    10201 tgattaccgg ccagcaattc accggcatct aaaaaatgac tttcaacaat tccgttaata
    10261 gtgatagtct gattatcacg aaatatatag ttgtaaaaat taagaatatt tgcattgatg
    10321 acaacatcac tttgcccggc aagaattttt aaagattcaa cttttacatc agcattgtta
    10381 aaattaattt taccactgat gctattaact gcaatatttc tttttttaag tttaaatttc
    10441 agattgttta atgtcagtgt accattggag cgataagtat ctttcttgtc tgcttgtccg
    10501 ctaattctac catgaagaga aacagagcct tgttgttctt ctgtaaagtc agcaggtaaa
    10561 tagcgactta aactggcaag gctaatgtca gcatctaact gtatgttgaa gtatggattt
    10621 ttaagatttt cgagataaac ttcgccaata attggtttgc cttcaattct tgcagaaata
    10681 tttttcagac tcaaaacact aatatttttt ccggatttac gattagaata aaatcctttt
    10741 agtgcaacat tcttcaatgc ataattttca ttatcaggta taatgtcggt tttattagtg
    10801 ccaaagttaa tttcaatggt tggtgattgt tttgatgatg caacaccatt tatatgtgta
    10861 tcaaagttta tgttaccatg atagcgatag tttaaaattt gcggtgcaac ccaattagat
    10921 ggtaaaatag aaagtagttc tgatgcattg gcttttgtag cagtaaattt aagatcgaga
    10981 aaagtttcat ttcttttttc aatttttcct tttgcattta tatttaagtt ggcaagttga
    11041 atttttaaat cattaaatgc ataaatgtca ttacgtaggt caaccgaaac aacaccatta
    11101 agttgtatgg gcttttgttt gatgtaagaa atattttcat gttgaataat ttcagcaaac
    11161 aaatctgttt ctgttttaag ttcaaatgtt ttatccttaa aattacctga agcaaggctt
    11221 ttgttcaggt gagtttgtaa gatgaaatta gatggtttat tttcaatact cagattaaca
    11281 tttttaagaa ttactttttt gaattcagca gcgaaggagt tgttgtcgtt tccctgtttg
    11341 aaaatctgat catttttttg accgttttca ttggtcagaa tatttaattc agcatcagaa
    11401 atctctatct ttttcagctg tattgttgaa gaaaatatgc tggtaatgct aagcaccaaa
    11461 tcaatttttc cggcttttag caaagggcgt tttgtgtctt gccaaggatt gccttttatg
    11521 actacatttt taagtgtaag tgatgcattt ggaaaaaaac gtagtacgga aaaatcaatt
    11581 tcactaacac ttgcaggtgt gttgaggctt ttatttattt cttctattac agattgtttg
    11641 attttatctt gtaataagac tgaaagtccc attgcaatga gcagtagtgc caagcatgtc
    11701 aacagtaaaa aaacaaagta gcgcaaatac ttattcatgt cctcttaaaa cgttgcaact
    11761 tttttaaatt gtaataacga taaaaatgta ttttgcaaag taattttatt agcacatagt
    11821 aaactatgaa ataaggcgtt tgttttatgg agttttaaac ttttaatgtg caataaaaaa
    11881 ggctgcctta attcaattaa ggtagccttt ataaattaat taatcgggaa attatttctc
    11941 gaaacgattt gacacttcgt cccagttaac cacattccag aaagcagtac agtaatcggg
    12001 tctgcggttt tggtagtgca ggtagtaggc atgttcccaa acatcaatac ctaaaagcgg
    12061 tgtacctttt acttcagcaa catccattaa cggattatcc tgattggggg tagaacaaac
    12121 ttcaagttta ccggcagcat tttttattaa ccaggcccag cctgatccaa aacgagttgc
    12181 tgcagcttga ttaaacttcg ttttaaattc gtcaaacgaa ccaaaagcgc tgtttatagc
    12241 gtcagctaat tttccttttg gtgcaccacc tgcattgggt gccataatgg tccaaaacag
    12301 tgagtgattg tagtgtccgc caccattgtt gcgtactgcc atcggtagtt tcgaaatatt
    12361 tttacaaata tcttcaatgc tcattttttc tgcatctgta cctgcaatag cattgtttaa
    12421 attggttaca taagcctgat ggtgcttgtc atggtgaatt tccatggtgc gtgtatcaat
    12481 atgtggctct aacgcatcgg gtgcgtatgg tagtggaggg agttgaaaag acataatatc
    12541 taatttaaat gttaagcaac aaaggtagtg aaatatgcta cctttgttgt caatcaagta
    12601 ctattttcag aagaaataca ttgatacagg ggttgttact ctccggctaa aattataaaa
    12661 cactgataac caacgaataa aaatttaata taaaacttaa tatgcagagg taaagcaatt
    12721 tatatttgcc gctcaaattt caaatcaatt aattacaaaa caatgaaaaa agttcttgcc
    12781 ttagttatgg ctgccggaat gttcagcatc gttgcatgcg gtccttctgc tgaagaaaaa
    12841 caaaaaatgg aagaaacagc taaagctgct caggattcaa ttgctgctgc tgctcaacaa
    12901 gctgcttctg ctgctgaaca agctgctact gacgctgctc aggttgctac cgatgctgca
    12961 caaactgtag attcaactgc aggtgctgta gtaaatcagg ctactgaagc tgttaaagaa
    13021 gctgctacaa agtaatcagc ccccatttta aaagatataa gctcgcttta gcgggctttt
    13081 cttttttata tcaaatcata aacctttccc ggataatttt ttatcacacc tttcaattct
    13141 agattcaaca acaatgcagg aatacggttt agcttaacac ccgatcggaa acagattgta
    13201 tcaatatcaa cctttgagtt ttctttcaaa aaatctacca actgtttttc ttcaggattt
    13261 aagtcttcaa acaaacgtgc ttgtttagga atagatttca catctgtttt atcccatccc
    13321 ataaaatatt caatgtcggc agcagattga attaaattgg ctttattgtc tcttatcaag
    13381 gcattgcagc cttttgaata ttctttatca acctgaccgg gaatggcaaa aacatctctg
    13441 ttataagagt tagcaatgtc tgcagtaatc aaactaccac ctttaattcc cgattcaaca
    13501 accactacag catctgccat gccggcaatg atgcggtttc tctttggaaa attttctttg
    13561 tctgcattag tcttactcat aaactcgctg ataacagcac cttctgcctg cattttttct
    13621 gcagtgttgc gatgttctgc cggatacagt gtatcaagac catgtgcaac aattcctgct
    13681 gtaggtattt tacattttaa acttgctttg tgtgctgtaa tgtctatacc aaaagctagt
    13741 ccgctgacaa tcatcattcc atctgtttcc gctaaatctg caatgagttt ttcagtggtt
    13801 gtttttccat agtcgctggc ttttcttgtt cctacaaatg caatacattt ttccggattc
    13861 agattaccat tgcctttcac aaaaatcatt attggcgagt cattacatgc tttaagcctt
    13921 tttggatatt gatcagaagt gaaaaatagc ggttttattt tatttttctc aatgaaacga
    13981 atttcttctt cagccctctt tagcgactgt tcttttccat ttttaatttt atctgctact
    14041 atctgaccaa cattaggaat ttttagtaat gcctgttgtt tttctttgaa aatagcctct
    14101 gcaccaccac aatacccgac taggtttttt gctgtaatgt cgccaacacc aggtaagagt
    14161 gtcagcgcaa gctgatattg taaaacatcg gttgttaaat tattttccat tgttgacttt
    14221 caagattttc aagaatagtt taggtttgtg ccttcacaat ctgaatctgc aaagaaaaac
    14281 atgtttagcc aaagaataac tttattttat ttttttttcg ttttaggaat ttattctgtg
    14341 aatgcacagc agcaacgtgt ttctggaaaa gttaccgatg gagagggtga attgcccggt
    14401 gtagctgttt atgccgatga aagccacgct actacatcag atgctaaagg tgagtttgtt
    14461 ttaatgcttg ataaagggag ttggacactt aaagcaaaat tactcggtta cgaaactttc
    14521 agcaaagtaa ttctagtagg cgattctaac tatgttcaaa tccatttgat accattaagt
    14581 aaacaattgc aatcagttgt agtgagtgcc ggaaagtttg agcagaaact ggaagaggtt
    14641 acagttacga tggagaccat taaacctaaa agctttgaaa caacaggcga caacaatgtt
    14701 gaaacagcac ttgaacatgt aacgggattg aatgtaaatg atgggcaagc aaatattcgc
    14761 ggaggcagtg gatttagtta tggtgcaggc agcagggtgc tagtgctggt tgatgatatg
    14821 cccatgctta gtgccgatgc caatgatgca aaatggagtt ttttaccaat tgaaaattta
    14881 gaacagatag aaattataaa aggagcatca tctgcacttt atggttcgtc agctatgaat
    14941 ggggtgataa atttcaggac aggttatgct aagtcggagc cggtaacaaa aattgtacag
    15001 tacaatggaa tttatgattc accacgaaga aaggagatta agtggtgggg aaaagaaact
    15061 cagatgttga gtggagcaaa tatgtttcat gcgcaaaaat ttggacaatg ggattttgta
    15121 gcaggtggac attatctgct cgatgaaggg tttagaaaag gtgaaacaga gaagcgagcc
    15181 agagttaata taaatacacg atacagattt ccgaagattg aaggactggc agtaggagta
    15241 aatgctaatt atatggaatc attcggtggt cttttcatta tatggaatga tgataccaca
    15301 ggtgcttata ttccaagtgg aggcgtgaat gattcaacat ctactttgag taattatcac
    15361 acggttagat acaatattga tccttttatt acttatcata gtaagaaagg ttttagtgca
    15421 aagtggcgct ctcgttattt ccggactttg aattacaatg ataccgatca ggaagctttt
    15481 gcagatttat attattcaga agtgcagttg caacagcgtt ttgaaaaatg gaatctggtg
    15541 ttaaccggtg gaggtgtgat tactgatggt ggtgtgcgca gtgatttgta tggtagtaga
    15601 aaaacatata acaaagcatt gtatgcacag gcagataaga aattttttga caggttgaat
    15661 ctttcattag gctggcggtg ggaacaattt cgtttccgtt acagtaaggc agaatccaaa
    15721 cctgtaatca gggcaggagc aaacctgcgt ttattacgtg ccacgtattt acgcagtagc
    15781 tacggacaag gatacagatt tccatcaatt gcagaaaaat atgtaaaaac aagagtaggt
    15841 tcaattgtta tttatccaaa cgattcaata ttttctgaaa gcggatggag ttccgagata
    15901 gggttgaggc agttatttgc tgtcggcaaa caattttcag gatatgtgga tgcatcggta
    15961 ttcagaagtg agtataagaa catgctagag tttgccttcg gaagttatgg tgatcctttt
    16021 aaagatcctc tgtttggact tggatttaaa tcactgaata ttggtaatac tcgtattgat
    16081 ggaatggagt ttgaacttgg tgtgcaggca acagttctta aaaaaataaa tcttcagttt
    16141 ggaggaggct acacttatat tgatccacgt ttgactgatt ttgattcttt aaaagcatca
    16201 acaggaacca ttacatacaa cttcttaaaa tatcgttaca gaaatttgtt taaaggttat
    16261 ggaagtatag cctatggaaa agtttcttta gctgtaaatt gcagatacaa tagctttatg
    16321 gaaaacattg atgttgtatt taatgaattg cttccagggg ttaaaaggta tcgggccgaa
    16381 cacgataaag gcgactggat ttatgatgca aacatcagtt ataaattaaa ctcccaaata
    16441 gcgttatccg ttatttcaag aaatttgttc aatcacgaat attccggaat tccctctcaa
    16501 ctcgaagcac caagaatgtg gttgtttcaa tttgtaatta caccttagtg ttttactatc
    16561 aatgttaaaa tcaaactaaa gttgtgtaag gcatcaactg ttcaggattt tatttttaat
    16621 cgtttttgaa acagcttcac cgattgactt aacatgaagt ttgtgatata tttttctgat
    16681 atgagtgtgg actgtttcgt aactaacttt aagttctgtt gcaatggttt tgtaggactt
    16741 accttttact aataattcca gtacttccat ttcgcgtttg ctaagattgt agtcttcatt
    16801 tttggtttcg gtaacttgtg ttttagcaaa acgagtcaga acttttcttg caataaccgg
    16861 tgtcattggt gcgcctccgg tatagacttc atggatatgg tgaatcagaa gctccatgct
    16921 tgtgtttttc aaaatatagc cattagcacc agcacatatt gaatcaaaca ccttatcatc
    16981 atcttcaaag ccggtgagca ttaaaatagg taagccaggg aactgtttgc gaataatttt
    17041 tactgtttca atacctgaca atttaggcat atcaatatcc attaggataa catcaggttt
    17101 taaggtgtca acttgtttta tacattcttc accggaatca aattctgcaa ttaaaagcaa
    17161 tgatggtgtg ctttgcagaa gaagcgcaac agattctcgc atgtttttat tgtcatcaac
    17221 aataatcact ctcagtggct tatcgggaat tgttttattc atgctacaaa tttgaatatt
    17281 cttatttgat cttgcaatca ccaattctgg ttatattgca tgttgtttca ggctaatagt
    17341 aatttctgta cccttacccg gggcactgtt aatctggaac tcagcattta aatcttttgc
    17401 tctggaagcc atgtttttta atccattacc accatttcca tttgtttgcg aatgattatt
    17461 ggccatatct attccacaac cattgtcttt tatttttaaa aatgtcattc cacgatttct
    17521 gcttaaatgg atatcaacct gagtgcagtg tgaatatttt acaatattgt gaatagcctc
    17581 tttaaaaatc agaaaaagat tctttcgttg tgccatatta attttcaggt tctgaatatt
    17641 ctcttcaaca atgaaattaa aatttatatc agcatcgcct aaaatgcttg cagcataggt
    17701 ctccatacgc aatgaaaggt tagacatgtt gtcattactt gctttaacca cccaaaccgt
    17761 gtcaccggtt ttttcaataa tttcttttga cgactttttt attttctcta aaattccggg
    17821 aatttcatgg tgtttatctt gtttcatttt attatcggca atctgactgt agaggtgaat
    17881 actgcctaat gtgctgccta tgtcatcatg taaatcgcgt gcaatattgt ttcttacttc
    17941 ttgtaatctt agaagctgat tgattctgta acgataaatg aaatacacaa aggcaccaat
    18001 actaaaaact acaatgccat agaaccatat cgtgctggta aacggtggta caataacaaa
    18061 agacaaagtc ttaatgtcgc tttgcaaatt attgttcgaa atggctttaa catcaagacg
    18121 aaattttccc ggaggtaaat tggtgtaggt tattatagga ttattatcgt tgctgcgcca
    18181 attatcatca atacctgaca ggcgatagaa ataagttatt ttatcactgt tggtataaat
    18241 atttggtgac acataaactt ttaaaaaatt ctgattgtga tgaagtttga tgtcagagaa
    18301 aagtaatgaa tcagatggtt tgtccattac ccatagtttg gtaaactgaa ggtttggttt
    18361 ttctgttgaa gagagtaatt gtgatgggtt ataactgatg aatccaccat taaagccaac
    18421 acttagtgta ttgttgtcac caggtttatt ttgaaagaaa tagccgaata tattctgact
    18481 taaaccactg cttgcatcat acaatttaaa aaaatttgtt ttctcattgt attgtaaaag
    18541 acctgatgag gtaattatcc ataataagga gtctcctgta gatttgattc cttgcataga
    18601 tgtgatatca gaatcaatta ttttaaaagg agaggtttta ttttctttac taaaaagtac
    18661 caagcctgcc ccctgtgtcg aaagccaata ggttccatct ttacgttcag caatatcaaa
    18721 aacatttttt atttttattg gaattgagtt cggcaacata ttccaatttg ttggcatcac
    18781 cgttattggt aacatggaat cattatcagc tgcatatttt gataaactaa aatcgctctt
    18841 gaatttaagt tggacaagac cttttgatct gccacaaacc cagaaattgt tttttgaatc
    18901 aacccaaatt ttgcgactta aattctctaa attgtccgat atttttgtat tttcatggat
    18961 aagatagtac aagtttcgat tatcaaagtc aattaataca atgtagttgc catagtaaga
    19021 tgcagcaatt aattctgaat tgtcttgttt gaaactggtg agagaattga ctaatgatgc
    19081 accattaaag taaaatgaag tttctttcag atttttatta aagtctattg tattgaatga
    19141 aatattttca ggattaaatt tgaaaacagt tctgttactg ccaatgtaaa tatcaccatt
    19201 cttcgtcatc agaatactat ataattcctg ctcttcattt cgataaataa cagttttgaa
    19261 cttataggat tcatttggag attttttata aaataaacca cattgggtaa tgacaaggtc
    19321 taaacctttg tcagtaatga gtctgtcgaa tactttgcaa gcattaactc cttttggcac
    19381 aggaagcatt tctgttctga attgatttct atgctgatca tacactaaca aaccgttttc
    19441 tgttgcaatc cagattctgt tttgtgaatc acgaaacaat tcgtttacac gggtattaat
    19501 ctgatgagaa aaatttatat tgtttataaa tgatttagaa gctttgttaa aaataaatat
    19561 gcctgatgat tttgttccac accagatgtt attgctggca tcagaagaaa ttgaaataat
    19621 ctcttcgcca gattttttaa atttagatgt tccgtcaaag atgtacgatt ggaggcgctt
    19681 accatggata ttgaatagat gcagtgcatt atcccatgcg ccacaccaaa cattcaaatc
    19741 attatcaata taaattgtat taatagttgt agctgtatca ccaaccatac ccttattcct
    19801 tccaacgtta tctaattttt ttagtgattc tattatacct gcaaaaagtg aaatagtgtc
    19861 tttaacagca acagcgtaat atgagttgat tgaatataca tagggattga gttttttatt
    19921 tttcaaatca aattgttgca gacctccatt attcatggca acccaaatac aattacgtac
    19981 tttgtcaaac gctaaatcgt taatatattc agcacgaagg ggactgttgc gggcatgata
    20041 gttttgaaaa gtatttgttt tgtaattata actgcaaaaa ccattagatg ccgttccaat
    20101 ccataataaa gagtcgtctg taacttcaag gcaggtaata ttgttgccac taacagagtt
    20161 tttatcactt tgaaatttaa atacctgaaa actttttccg tcaaaacggt tcagcccatc
    20221 ttttgtacca atccagatga atccatattt gtcctgaatg acacaatttg tttgattgtc
    20281 ggataagcca tcattagcag taaattcatt gagattgatg gttaatgaat ccgccaatgc
    20341 aggtaaagta aaaaccagaa atagaagtag tgatttgaaa aatttaatca ttcggttttg
    20401 gcaaggaatt gcctaaaata aataaaatct tctatttaaa gaaaacgatt ggtgaaatta
    20461 aaagtagaag tggttaaact atggatttgt gtggtgtaat gatgtgttgt tgtttaatca
    20521 cacagaagct ttaataattt tagtcgtgta cagaaaaacg ctttgtaaca gaactgttgc
    20581 tattagaaat ttttaaaaca tatatgccat tagcaagatt ttgtgtttgg acatgaaaaa
    20641 tattttctgt tccatttaat tcttgcgttg aaattattgc tccattaata tcataaactt
    20701 ctgcggttaa attcttttcg acattacctg ttactttaaa atagaggttg tttttattcg
    20761 taaatacagc atagtttatt tgtagctgtt catagttgtt tagttcaaca gcaactgaat
    20821 tgctcaaatg gctttcacca ttaaaatcaa attgtcttaa acgatagaaa gattttcgtg
    20881 ctaatggttt tgaatctgtt gcatgatatc gtaaaagctg attagaaaat cctgcagcct
    20941 gtgttttatt aattggtata aagtttaagt caacagcagc acgttctatt tcaaagtaat
    21001 cactatttat ttcagatact gttgaccatc ttaattcaac ttggtcatta tttagttgtg
    21061 ctgtaaaatt cagcaactca acaggtaagg gattttctga agatgccgat gataaggtaa
    21121 atggtccaaa taaattgtct gctgtgcttg tagtaatggt acctgctgac acatttcctg
    21181 ttgtgctgcc attgcctaaa ttattccata tagttccaac actgcgccaa cgagcaactc
    21241 ttaaatcgct gagtaaggtt actccacaac tggaagcatc ccatgataat gttacatttc
    21301 gacttgaact tcctgcaatt tgattaagcg tccagaattc gcactgactg atgtggtcaa
    21361 gtgttggatc aaaaatatta ctgtatgtta tttgcggatt agcacgcata tattctgcaa
    21421 caaactgact tgaagttaaa gaacttagcg aaatatcatc tatggcaaag gatggatcgg
    21481 tgcctactcc atcatcattg ttttcccatt tcaaaccaat tcgcacatta gcattattat
    21541 ttgcacttgc cggtagtacc attgaaaatg ctgtccattt tccttgcctg aatccattac
    21601 atgctccacc gcaacatgca gtctttggca tatcggtaat ttgtgcccat gcggcaccat
    21661 cataatacca aagggtagca ttgtcaattg taccatcgcc aaattcaatg taattaaaac
    21721 tgatgctaat gttacttttt cctgtgcaat tgattacagg cgattgtgcc attctgtttg
    21781 tttgggggca gtataaaagt ccacataacc cacctgcatc atatgcagct ccacctgttg
    21841 gattaaatac actactaaca tgcatggtat tgtttccatt atttgcaact ccacaaccac
    21901 ctgcagcaac accaccttct gcatcactta ttacaaaagg atttggatca gcaccttcgg
    21961 gccccataac gttaacaaga gaccagcctg ctccggtatt aaaattttct gtccagaaag
    22021 atgacgatga tgcatcagcg ccaatagcaa ttgaccgata tgaagtattt tttcctacag
    22081 gaaaagtgaa tgcctgtgag ccgttttttt gcaccgggcc atttacaaaa ctaacatttg
    22141 atgctcctgt tgcagttgca ccattttcaa ctattaaaat attgggagtt gaagaattta
    22201 taatacctgt tattaagttc agattgttat caacctttac tgattttaaa agggtaaggc
    22261 tgttggcaga cttattcatt tcaagttgat aaatctgtag cgatgtaagt gctgcagagc
    22321 ctgatataga ttgtgcatta ccaccactga atctatactt acgtgcatta gctgcaccat
    22381 aacttgtgga gccatttaca gttaggtttc cgaaaatttc aaaccctgtt ccgaaatttc
    22441 tgagattgca tccaccacgt attagtatat tgccctgaac ttgtaccggt gtagcaaaat
    22501 tgttatcatt taaaaaatca acagtgccta atagtggagc taacacacca ccaacatcaa
    22561 aattaccttt gatagttgca gtagctgtgc tgcctgtgaa tgttgatgaa acagcagttg
    22621 tccaaacacc tcccgaagta ttcatgatag tgaggtttgg ataaaacata tttaaagttg
    22681 ttaatgaagg tgcagtaact gaactgggtg ttttatttaa aacccaactt gcagttgcag
    22741 ggatagtgct tatgccaccc tgataattgt tttgccagtt tgttgcattt gtattctctg
    22801 ttttaacata agtgcctgat gcacctaatg cccatgttgc tccagctagc caggcaacgc
    22861 tggttgaact tccatctgta aatgtaccgt taacctgtaa gtcaacacct gtaccattat
    22921 tgattgtaag cagtttggaa ttgtaattta cagttccacc attgttaata gttgtttcat
    22981 caatgcgtac attggccgaa atggttactg tatgtccact gcgaatggta atagttttag
    23041 catagtagtt tggtaaaaaa acagctgctg tccacgtaac taaatccggt gattgttgcc
    23101 atgttgataa tgaattccat gcaccggaag tgatactgcg ataatgattt tgtgcatcgt
    23161 tggctttaat aattacatca tcaattcttg atgaaccacc ggaagccaca gtgctgttgt
    23221 taacacttgt attcgttgtc attatccaac gtaaaaaaac tgaagcttga ttttcacatg
    23281 caacaggtaa ggggatatcg gtgacaacac ctgaagtaaa attgtctgcc acaacaattg
    23341 caccggcacc gggaattggt gtccatgtgc cggcaatacc aatcttatat tctatagcaa
    23401 agttcttggg ccctgtgggc gaactgcgtt gtttagagga taattcaaga ttaaaataac
    23461 ctgttgtatt aatttctgtc tcccaatatt ttgtgccgga gccattatcc catcctgttg
    23521 tccaagcact ttgagttgtg gcaccggcag ttgtataaac aactgcggca gtacctccta
    23581 cggtgtaaat ggtttttgca ctattagcag gaatagaaat atctacagta gcattatcag
    23641 gattgtttgg aaaattccat tcagccaatg ttaattgagc agaagcggct ttgacataaa
    23701 gtagaaatgc caatgaaata aataaattgg ctactctttt catgttgttt tttattgtaa
    23761 aatcaaagat atgagaaatt attactaatc caacatttaa tagataaaac ttatgaaagt
    23821 aaaaaactga taataggatg aaggcataag ggggtgaatg ttgcagaaaa ggatatcctt
    23881 aaaaaccgga gattttaaag gagattatgc aatgagtata taggaatggt gccttcaaga
    23941 aaggcttaca aaaaaacaag gctgcccaat cgggaagcct tgttgtaaga gaattatggg
    24001 gttaattaat cctttatcag tttataagtc tgaaccacat tgcttgaatg taattgcaac
    24061 agataaactc cttgagtgta gttagataaa tcaacagtag tttcattgcc tgaaaacaaa
    24121 gtattaatca atgtacgacc ttgtaagtct gttactgtta tctgaacatt ttcaagttga
    24181 ttgtcggatt gtacattaaa tactcctgaa gaaggatttg ggaagatgtt gagtaatgtg
    24241 ctgttgcttt caattttaaa agctggatga acaaaatcaa atgcaaattt tcttttgcct
    24301 tttttcaatt tcaacttcaa acccgaattt tgatggcaat cctgttttta aaatctcatt
    24361 ggagggttgt taaggattaa ctaactaaat aaacagtatt gttattgtca atatgtaacg
    24421 agtttacaga gaagcaagtg tttattgccc agacaaaatt tcctgctgca tcaaatttga
    24481 ttaagaaaat tgcattgacg ttgttaccgc ctggtgctgt cagatttaaa acagctgggc
    24541 ccggattaaa gtcaacagtt cagaaaagaa ctggttacat aaaggtcacc gttaaaggga
    24601 tcttttgtaa ttgattttcc tttttcatta gaggccactc ccatagtctt tacccatgat
    24661 aaagatggca tttgtgaatt tgatattgta aatgatacaa caaacaaaca tgccattaat
    24721 caggatactg atttgttgta aaatttattc atgacttttc agtttatatt ttagtgaatg
    24781 ataatttttt ctgttaagat attttcctcc ggagcactaa cagaaaataa atgaacgaaa
    24841 taaattccac tttctaattt gcttacatta attgctgttt ctgcacctcc aacaactgta
    24901 tgtaaaactt cttttcccat cacatcaaaa atttttattg cactgcctat ctcaaacttt
    24961 aaattgttta tctttaaaat gtttgttgca gggttggggt aaacggtaaa cttgttttgg
    25021 atcatgtttt catatatgcc tacattattt tgattcatct taagcacata aacattggaa
    25081 agggtattga cagtagtaag attattaaca ccggtattaa aatcaaaatc ggcagtgcgg
    25141 ctaaaactgc cagcggtaaa aatgttgccg gatgcatcgg taacaatttc ttctccggca
    25201 tcgttaccta tgtcaccggc acggcctccc cataaaaaat tgccgcctgc atctaatttt
    25261 aaaatataaa tatcggggct gctggaggca gcagttacaa aagcagaatc aataccgggg
    25321 tccaaatcag cagtgcctgc aaacgaacct gttatatata cattgccggc aacatctgtt
    25381 gcaatgccat tgcctgcatc ataaaacgaa ctacttacta tttgttttgc ccataagaaa
    25441 ttaccggcag catctgattt taaaacaaaa acatcttcaa aaatactggt ggtaagatta
    25501 aaagtgccat tgccccattc aaaatctact gcgtcttcaa aataacctgt tgtaaataca
    25561 ttgccattgg catcaacact tattgcctgg cccacatctt cggatgttgc tccaaaattt
    25621 tgcgcccaaa taaaattgcc tgttgaatct aactttaaaa taaaaatatc ggggtcgctt
    25681 gccacaaact gattgctgcg cggagcaatt aaattaaaca cacctgcacc cgggtcaaag
    25741 tcaaccgtat cggaaaaaga gcctgttgcg tgtacattac ccaaggcatc aatagctatg
    25801 gaatttccaa tgcagtaatt gaaccctcca aaattttttg cccacacaaa atcacctgtg
    25861 gcatttagtt ttgaaataaa aatgtcgcaa tatgggccag cattaagcgt aacaaccccg
    25921 ggtcctgggt caaaatcggc actaagttca aagctaccta tggtgtaaac atttccccaa
    25981 ttgtcggttg taatatcata acccacatcc cggctgttaa tagatgatgc cgtactgcct
    26041 atattttttg cccatacaaa atttccgttt gtgttaagat gccatataaa tatatcacgc
    26101 ccaccgtttg aggtcaggtt aaatgtggca gagcccggat caaaatcaac cgagtcctta
    26161 aaatgccctg taacaaatac atttcctgca tcactgcatg taagcgcaac ctcacttaaa
    26221 tttccaatcc atccggtggt tttaacagcc cattcaaaat tgcctgctgt atccaacttc
    26281 aacacaaaaa gctctaagcc aactgaactt gttaaattat acacaccagg gccgggatca
    26341 aagtcaaccg ttttaccgaa gcaaccggct acatataaat ttcctgctgc atcgcgtgca
    26401 atagatcttc cttggtcacc tgccgaatcg ccaacttgtt gtacccattc aaatgccggt
    26461 gtttgtgcct gcacaagcag ggttgttgcc attacgaaaa tggtaagcag tgtttttttc
    26521 atttgacttt agtttaatga tgaatgatta atttttgcga tgcacctgat gacttcattg
    26581 aaggagagga gtatgaaagc tgtagaaaat aaattccgtt ctccaatttg cctacatcaa
    26641 ttgttgactg attaccgctg atggtggatg tatgaacttc ttttcctgtt acatcaaaaa
    26701 ttttcagttc gcagttttct tttaataatc catgattaac taccaataac tgattagcag
    26761 gattgggata aagaatgaag taattttcat cagttaaatg agctaaacca actgtgctaa
    26821 agttcatttt ctgaataaag gcatcagtat ttccgatatc atttaaagtg gtagtgcctg
    26881 tgccagggtc aaaatctata gggccgtaaa aagttccggt tacgtaaaag ctacctgtag
    26941 gatttacaca aatagcattg gcttcatcat caccattaga tggacctcca atttcataag
    27001 cataaacaaa atttcctgtt gctgagagta tgagtacaaa tgcatcatag ctgctaaatg
    27061 taccgttgag gttaaatgta actgttggat cagggtcaaa atcaacaatt gaagatttaa
    27121 agcggcccga aaaatataca gaacctgcat tatcagtagc aatatccaaa ggtacatcaa
    27181 tagagttgcc acccactctt ttaacccata caaaattggc tgaagcatct aattttagaa
    27241 aatacatatc agacatattt gttcctgtcc ctacagatgt aagattagca gtgcctgtac
    27301 cagggtcaaa atcaactgtt cctgtgaatg ttccggtaac gataatgttt ccggctgcat
    27361 cagtattgat agcatatgca tttccgccat ctgtacctgt tgttccacca atatttttaa
    27421 cccaaatgaa atttgcagca gtatcaaatt tcgcaatata aatatctgta gcaccaatag
    27481 gaataacatt aaaaacttga gtggcgttag gattaaaatc agtggtgtca ttaaatgatc
    27541 cagctaaata aacagtattg ttattgtcaa tatgtaacga gtttacagag aagcctgtgt
    27601 ttattgccca gacaaaattt cctgctgcat ctaatttagc cagataagga tcctggccac
    27661 cagtagatac gaggttatac acacctgcag aaggatcaaa gtcagtggtg gtttggaagg
    27721 tgccttcggt gtaaacattg cctgaagcat ctaaagaaag ttcatcaacg aatgttccat
    27781 atgttccacc aaagttttta gcccaaacta aatttcctga tgagtttaat ttaactatac
    27841 aaacatcaaa agcgcctgat gctgttatga ttgatgttcc cgctcccgga tcaaagtcaa
    27901 cagctcctgt aaagtgtccg gtaaagtagc aattgcccga tgcatcaagt gccattgcca
    27961 cagcagtttc attaccagtg cctcctatat tatgcgccca taacagattt ccggtagtgc
    28021 tgtatttagc aatgaatatg tcatcatttc cattagaagc aagattaaca acgcctgctc
    28081 cggggtcaaa atccgcagtt cctgctaata ttcctatttc ataaacattg ccggcagcat
    28141 cggttgcaat atcatagcct ttatcgaaat tagtactgcc tatgttaata gcccagtcga
    28201 atgttggcgt ttgtgcattt agcgttagtg ctgaaaagca acaaagtgta agtagtgttt
    28261 ttttcatctg tatataattt ttgaatttta aattgcaaga ctttttttta taagaatcaa
    28321 ttgctgattg tgatttttaa atatagagaa tttaattgtt acactgattt agtgctgtat
    28381 gactaatttt tgataagccc cagcactttt acctaaagag gaagagtttg aaatttgaac
    28441 ataataaatt ccatttttaa tattggttac gtcaattata gatttctctc cgattacgtt
    28501 cgtctttaaa acttctttac ccatcacatc aaaaattctg agttcataag cgccatcaaa
    28561 catcagacct ttaacctcaa atttatttgt ggcaggattt gggtaaatat ttatgttaga
    28621 attatcatta atattatcaa tacctgttcc ggaattatat tttaaaatta caccatcgta
    28681 gcctaccacc catcctaagc cggcatttat aaaatatgag tcagtggttc ttgcatttac
    28741 acccgtgggt tctacctgat aggttacccc tccatctgtg gtttttctga gagtattgtt
    28801 tgagggtagc gaagcttcaa gattaatgta tcctacgctt gatgatacat aatgtacagc
    28861 aataatgctg gtttggaaac taaaagaaga agagttccat gttatgccac catccattgt
    28921 tttaagtccg attgagtttt ctcccacaac ccaaccttcg gttgcattta taaaatggac
    28981 atcgttataa tggacaacag gtgcagtttg ctgagaccaa gtggtacctg cattagttgt
    29041 atgtaatatt aaaggatatc ctacagccca tccttcagtt ggtgaaataa aatatacagc
    29101 cttgaaagca ttattcattc ctgattgtgg tgtccaggta gtgccgccat taatggtatg
    29161 tagtatggtg cctgaattac ctactaccca tccctctaaa gggttaacaa aaaaaacatc
    29221 aagcaaaagt tgtgtagtgc ccgatgtttg agaaacccat gtagtgccac cattggttga
    29281 tttaagtata gttccgccta agccaacaga atatgcagtt tgggagtcag caaaaaaaat
    29341 atcaaatagg tttactgtta ctcctgatgt ttgattagtc catgtggttc ctccattagt
    29401 ggtggttaaa atagttccgt taaatccggc agcacccccc acattggcat caaaaaagtg
    29461 aacgctattc aggtcttcgg tagtgttgct tgtctgctgt gtccatgttt gtgcttttat
    29521 actattcatt gataagctac caacaattac caatgtgaaa attaatttta atgtgtctaa
    29581 atttctcata ttctttggtt taaattttaa aaacaattgt gacccgaaat atcggaatta
    29641 ttttttacaa ataaatattt atcaaaaagt ttctgcaatc accaattctg gtgatttttc
    29701 cggttaataa tggtgtagtt aagttcgtaa aacaaaaaca aggctgccct ttgggcagcc
    29761 ttgttgcaaa acaattagaa tgctaaatta ttactcaaca acaattttaa tttgtttagc
    29821 aaactgattt gattttacac taatcatgta agcaccttta gctactttgg ttaaatcgag
    29881 ctgcatagta ttatttcctg cagaaacgtc ctgagatgaa ttcaggattg ttctgccact
    29941 taaatcaatt actgaaactg atgcattgcc cttttcaact gcattgaatt caacattcaa
    30001 cataccggca gttggattag gatatgcagc tatagaacct gcatcaactt gttgtgcttc
    30061 tttacaacca atgttcgcta cgtaggcttt agaaccttca ccacaggagt tgcttgcttt
    30121 aataataaca gttccattac ctgtgcccca atcaagagtt aatgagttgg agtttccacc
    30181 acctgaaaca taacttgcag taggcgcatt aggaaatgtc cagtttaaag tataagcacc
    30241 tgtagtatta ctcatgtcag catcgaactg aataccttgt gtgtttgcac acc
//
LOCUS       DAORQT010000002        27379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_84_length_27379_cov_15.933721, whole genome shotgun sequence.
ACCESSION   DAORQT010000002 DAORQT010000000
VERSION     DAORQT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27379
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84_length_27379_cov_15.933721"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="rplP"
                     /locus_tag="PK134_00095"
     CDS             <1..272
                     /gene="rplP"
                     /locus_tag="PK134_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRB37283.1"
                     /translation="VALTRFMKREGQTWIRIFPDKPVTRKPAEVRMGKGKGAPEYWIA
                     QVKPGTMLFEADGVSVETAKEAMRLAAQKLPVITKFVIRRDYSAA"
     gene            292..492
                     /gene="rpmC"
                     /locus_tag="PK134_00100"
     CDS             292..492
                     /gene="rpmC"
                     /locus_tag="PK134_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRB37284.1"
                     /translation="MKQSIVKDLTTEEVKSRIKEEVDNLQKHKMQHAVSPMDNPMKIR
                     STRKLIARLKTELNKRMSQTSK"
     gene            501..761
                     /gene="rpsQ"
                     /locus_tag="PK134_00105"
     CDS             501..761
                     /gene="rpsQ"
                     /locus_tag="PK134_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRB37285.1"
                     /translation="MSTERALRKTKIGVVTSIKMQKTVVVAEEKKFKHPMYGKFINKT
                     KKFHVHDETNACGSGDRVLIMETRPISKSKRWRLVEILEKAK"
     gene            812..1180
                     /gene="rplN"
                     /locus_tag="PK134_00110"
     CDS             812..1180
                     /gene="rplN"
                     /locus_tag="PK134_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRB37286.1"
                     /translation="MIQSESRLTVADNSGAKEVLCIRVLGGTRKRYASLGDKIVVTVK
                     NALPAGNIKKGAVSKAVVVRVSKEVRRNDGSYIRFDDNAVVLLNQADELRGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            1198..1515
                     /gene="rplX"
                     /locus_tag="PK134_00115"
     CDS             1198..1515
                     /gene="rplX"
                     /locus_tag="PK134_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRB37287.1"
                     /translation="MSKLKIKKGDIVKVIAGESKGKQGRVLEVFPETNRASVENVNIV
                     SKHTRPNAQNTQGGIVKKEAPVNVSNLMLIDPKSGKPTRVGRKTEEGKLVRYAKKSGE
                     VIK"
     gene            1515..2072
                     /gene="rplE"
                     /locus_tag="PK134_00120"
     CDS             1515..2072
                     /gene="rplE"
                     /locus_tag="PK134_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRB37288.1"
                     /translation="MSYTPRLKGKYKKEIISALKNHFQYKSIMQVPKLSKICLSQGIG
                     DAVSDKKIVDACVAEMTAIAGQKAVATYAKKDISNFKVRKGVPIGVRVTLRDEQMYEF
                     LDRLVSVSLPRIRDFRGINNKGFDGKGNYTLGITEHIIFPEINIDKVSKIRGMDITFV
                     TTAKTDNEAMQLLKEFGLPFRTGSN"
     gene            2089..2358
                     /gene="rpsN"
                     /locus_tag="PK134_00125"
     CDS             2089..2358
                     /gene="rpsN"
                     /locus_tag="PK134_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRB37289.1"
                     /translation="MAKESIKARSRKREHLVEKYAAKRAALKEAGDYQALALLPRNSS
                     PVRLHNRCMITGRPRGYMRQFGISRVLFRKMALEGKIAGVTKASW"
     gene            2389..2787
                     /gene="rpsH"
                     /locus_tag="PK134_00130"
     CDS             2389..2787
                     /gene="rpsH"
                     /locus_tag="PK134_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRB37290.1"
                     /translation="MTDPISDYLTRLRNAVKANHRIVEVPASNTKKEITKILFDKGYI
                     ANYKFEETDNKQGNIKVALKYNPVTKLPAITRLKRVSRPGLRKYVSHDRLPRIINGLG
                     VAIISTSKGIMTDKEARQLHVGGEVLCYIY"
     gene            2799..3353
                     /gene="rplF"
                     /locus_tag="PK134_00135"
     CDS             2799..3353
                     /gene="rplF"
                     /locus_tag="PK134_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRB37291.1"
                     /translation="MSRIGKLPVAVPSGVQVNVQGNTVSVKGPKGTLSQTIADGITLE
                     MEENKVVVKRSTEQKRHKSLHGLYRSLLANMIKGVSQGYKIEQELVGVGYRASNQGQM
                     LDLVLGHSHHVVFELPTEVKVSTKTERGSNPMIILESHDKQLIGQVAAKIRSLRKPEP
                     YKGKGIKFVGEVLRRKAGKSAAKK"
     gene            3367..3717
                     /gene="rplR"
                     /locus_tag="PK134_00140"
     CDS             3367..3717
                     /gene="rplR"
                     /locus_tag="PK134_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRB37292.1"
                     /translation="MKNSKDSRRLKIRKRIRGKIKGTNEQPRLSVFRSNKQIYAQIID
                     DVAGQTLAAASSAENGAAKGTKVEISTSVGKMIAERATAKGITKVTFDRGGYLFHGRV
                     KALADGAREGGLKF"
     gene            3727..4245
                     /gene="rpsE"
                     /locus_tag="PK134_00145"
     CDS             3727..4245
                     /gene="rpsE"
                     /locus_tag="PK134_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRB37293.1"
                     /translation="MIATANKRVKPGEIELKDRLVAVNRVTKVTKGGRTFSFAAIVVV
                     GDEKGVVGYGLGKAKEVTDAIAKAIDDAKKNLVKVTVHKTTVPHEQDAAFGGAKVFLK
                     PAAPGTGVIAGGAMRAVLESVGIHDVLAKSKGSSNPHNVVKATITALAMMRDAVAVAN
                     QRGVDLNKVFNG"
     gene            4256..4438
                     /gene="rpmD"
                     /locus_tag="PK134_00150"
     CDS             4256..4438
                     /gene="rpmD"
                     /locus_tag="PK134_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRB37294.1"
                     /translation="MGKVIIKQVKSGIDRDKNQKLTLKALGLRKVYHSVEKENTDQIK
                     GMIEKVRHLVEVTEIK"
     gene            4449..4901
                     /gene="rplO"
                     /locus_tag="PK134_00155"
     CDS             4449..4901
                     /gene="rplO"
                     /locus_tag="PK134_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRB37295.1"
                     /translation="MNLSNLKPAKGSTKKSKRIGRGEGSGHGGTSTRGHKGAQSRSGY
                     SRKFGFEGGQMPLQRRVPKSGFKNFFRVEYTGINLDVLQKLNEKFGLTEIDHSILCEK
                     GLIAKRELVKILGRGELKVKMNVKAHAFSETAKNAIEAAGGTTTIQEK"
     gene            4901..6262
                     /gene="secY"
                     /locus_tag="PK134_00160"
     CDS             4901..6262
                     /gene="secY"
                     /locus_tag="PK134_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRB37296.1"
                     /translation="MKELIQTLRNIFKIEELRTKLLNTLFFIAIYRLASHIVLPGVDP
                     QQLGNLKNQASQGIFGLLDMFAGGAFSQASIVALGIMPYISASIVVQILGIAVPYFQK
                     MQKEGESGRNRLNQITRWLTVLVVAGQAPGYLVNLRAQAQSAIVAYSNPDAMSPTLWW
                     ITSIIILIAGTLFVMWLGERIQEKGIGNGISLLIMVGIIARLPFALRDEFLSRLNQTG
                     GGLIMFLVELVALFAVVVISIMLVQGTRKIPVQFAKKIIGNRQYGGVRQYIPLKVNAA
                     GVMPIIFAQAIMFIPATVAQFFPESSSMQGIAHSLTDFHSVWYNVTFAILIILFTYFY
                     TAITVNPNQMADDMKKNGGFIPGIKPGKQTASFIDDVMSKITLPGSIFLAIVAILPAF
                     ATILGTSTNFAQFYGGTSLLILVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRTSIN
                     APV"
     gene            6271..7095
                     /gene="map"
                     /locus_tag="PK134_00165"
     CDS             6271..7095
                     /gene="map"
                     /locus_tag="PK134_00165"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRB37297.1"
                     /translation="MVQIEYRSKEEIDLIRVSSLLVGKTIAEVAKHLRDGITTGELDK
                     IAEDFIRSHEAVPAFKGYHGFSGSLCISVNAEVVHGIPGARVLHPGDIVSLDCGVVKN
                     GYYGDSAYTFAIGEVDQQAVKLLQVTRECLYLGIEKAVAGNRMGDVSSNIQKHAEANG
                     FGVVRELVGHGIGKKLHEKPEVPNYGKAGQGLLLKPGLVIAIEPMINMGTKNVKQLND
                     GWTIVTADQKPAAHFEHTIAVDNVKAEILSSFEEIERSEKENLNLYKIDYKQHIIA"
     gene            7109..7327
                     /gene="infA"
                     /locus_tag="PK134_00170"
     CDS             7109..7327
                     /gene="infA"
                     /locus_tag="PK134_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRB37298.1"
                     /translation="MAKQTSIEQDGTITEALSNAMFRVELENGHEIIAHISGKMRMNY
                     IKILPGDKVKIEMSPYDLTKGRITYRYK"
     gene            7361..7477
                     /gene="ykgO"
                     /locus_tag="PK134_00175"
     CDS             7361..7477
                     /gene="ykgO"
                     /locus_tag="PK134_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRB37299.1"
                     /translation="MKVKPSLKKRSVDCKIVRRNGKLYVINKKNPRFKQRQG"
     gene            7523..7900
                     /gene="rpsM"
                     /locus_tag="PK134_00180"
     CDS             7523..7900
                     /gene="rpsM"
                     /locus_tag="PK134_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRB37300.1"
                     /translation="MARIAGIDLPRNKRGEIGLTYIFGIGRSTAQQILDECGISYDKK
                     VDQWNDDELNKIRSFINDNLKIEGALRSETQLNIKRLMDIGSYRGVRHRLGLPVRGQR
                     TKNNCRTRKGKRKTVANKKKVTK"
     gene            7953..8357
                     /gene="rpsK"
                     /locus_tag="PK134_00185"
     CDS             7953..8357
                     /gene="rpsK"
                     /locus_tag="PK134_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRB37301.1"
                     /translation="MAKQQQASAAKTAKKKVVKVEATGEAHINATFNNIIISLTNLQG
                     QVISWSSAGKMGFKGSKKNTPYAAQLAAADCAKTAFDLGLRKVKVFVKGPGAGRESAI
                     RSLNASGVEVSEILDLTPIPHNGCRPPKRRRI"
     gene            8405..9010
                     /gene="rpsD"
                     /locus_tag="PK134_00190"
     CDS             8405..9010
                     /gene="rpsD"
                     /locus_tag="PK134_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRB37302.1"
                     /translation="MARYIGPKTKIARKFREPIFGPDRYFEKRSYPPGMHGAAKKRAK
                     QSEYAIQLAEKQKVKYTYGMLERQFARFYSIALRKEGITGENLLKLIESRLDNVVFRL
                     GIAPTRAAARQLVGHCHITVNGKVNNISSYILRPGDVVGVRERSKSFEAITDSLASRS
                     NKYGWLEWDTTAMQGKFVTTPAREEIPENIKEQLIVELYSK"
     gene            9075..10067
                     /locus_tag="PK134_00195"
     CDS             9075..10067
                     /locus_tag="PK134_00195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRB37303.1"
                     /translation="MAILAFQRPDKVIMNHSTETFGQFEFRPLEPGYGITVGNSLRRI
                     LLSSLEGHAITNIKISGVDHEFSTIKGVVEDVTEIILNLKQVRFKKQIEGTDSEKVQV
                     VVNGKTEFTAGDIAKHTSAFQVLNPELVICQMDPSVKLVIDMTIDKGRGYVPAEENKS
                     NSHPLGTVAIDSIYTPIKNVKYAVENYRVEQKTDYEKLVLEITTDGSIHPKEALREAA
                     EILIHHFMLFSDEKITLDTKSKTPVEEFDENTLHMRQLLKSKLVDMDLSVRALNCLKA
                     ADVETLGDLVSFSKTDLLKFRNFGKKSLTELEELVRSKNLTFGMNIQKYMLDKE"
     gene            10093..10575
                     /gene="rplQ"
                     /locus_tag="PK134_00200"
     CDS             10093..10575
                     /gene="rplQ"
                     /locus_tag="PK134_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRB37304.1"
                     /translation="MRHGKKINHLGRTHSHREALLSNLASSLILHKRINTTVAKAKAL
                     RKYVEPLITKSKSDSTHSRRTVFSYLQNKEAVSELFREVAPKVNERPGGYTRILKTGF
                     RPGDAAETCMMELVDFNELMPSSKEKGTAKKAKTTRRGRKPKAKADAAGSETTEATEE
                     "
     gene            10669..12279
                     /gene="bshC"
                     /locus_tag="PK134_00205"
     CDS             10669..12279
                     /gene="bshC"
                     /locus_tag="PK134_00205"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRB37305.1"
                     /translation="MFVQNTSIPFADAGYSALITDYLNNNIFLKEYASFQYDESGFKE
                     ALQKRVKYKANRDRLCEVLEQQYSETIFQTDWKTDKSYSAIKSLKEENTFTVTTGHQL
                     NIFTGPLYFIYKILTVIKQAEFLAQRNPGYKFVPVYWMASEDHDLAEVNHIDIETSKI
                     EWQTNQKGAVGRMTTEGMYSVVEALKNQLNKNDNFQELATILDTAYLKNATQANAIRS
                     LVHSLFSKYGLVIIDADDKRFKQQAIKLFEDDLFNNTSFNCFKNLSDFEKAYGLQIHA
                     REINLFYLVNGDRSRIIKNGERFEVADSGLSFSSDELKKHINEYPERFSPNVVLRPLY
                     QEIILPNIAYTGGPAEVHYWLQLKSIFDKLKVFYPMVVLRNGMMVVNADQATVLNESA
                     LEWKDLFHPTEQVVNIALKSKYGEELSLKEEKDTLQRSYEEMEQRFKAIDITLEKHVK
                     AEWRRTEKRLMASEKKLLRAFKKKQVDETCKIRNTVKQLFPNGKLQERKENVFPYLSK
                     YGLQFIDDLYKICEPLPTNFKIAVTAEK"
     gene            complement(12286..13461)
                     /locus_tag="PK134_00210"
     CDS             complement(12286..13461)
                     /locus_tag="PK134_00210"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRB37306.1"
                     /translation="MDAYIVAGYRTAVGKAPRGKFKNTRPDDLAVTVIKHLIQSVPNF
                     DTELIDDVIAGNATPEAEQGLNIGRMISLMGLNTVKVPGMTINRYCSSGLESIAIASA
                     KIHSGLADCIIAGGVECMSPIPFGGWRIVPNYEVAANNPEWYWNMGLTAEAVAKEFKI
                     NREDQDQFAFQSHQRAINAIKNNLFKEQIVPVEINETYLDANEKRQTHKSTVDTDEGP
                     RQDTTIEKLNKLKPVFDAKGTVTAGNSSQTSDGASFVLVVSEKMLKTLNVKPIARFVS
                     YAVSAIEPRIMGVGPVYAIPSVLKRAGLKQNDMELIELNEAFASQSLAVIRTLGLNPE
                     ITNVNGGAIALGHPLGCSGAKLSVQLLNEMKRRKMKYGMVTMCVGTGQGAAGIYELL"
     gene            complement(13519..14163)
                     /locus_tag="PK134_00215"
     CDS             complement(13519..14163)
                     /locus_tag="PK134_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37307.1"
                     /translation="MKILKQLLLLLILLPAFAFAQREEKPRYAPGREKHRNKTDELGQ
                     RQGVWKYYNEAGELNQEIEYLDDVRNGIAKKYYPYGKIMEEIEYANGIKDGEYKRYYY
                     NGQAKMEGEYLNGKKSGTWTTYFSDGEIRNTGPYKVNLKNGLWKVYDRKGKLISQVEY
                     KMGEDAKALAAAKEADEKAKKAAAAKKAADDKKKLPPGAKLKTPSPPPSTPEKK"
     gene            complement(14368..16773)
                     /locus_tag="PK134_00220"
     CDS             complement(14368..16773)
                     /locus_tag="PK134_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HRB37308.1"
                     /translation="MRRKIEKVAVLGSGIMGSRIACHFANAGFNVLLLDIAPKEFTEA
                     DKKAGFVEDSPKFRNKITDTHLQSAIKSNPSPLYHKSFAQRISIGNFTDNLKDIANFD
                     WIIEVIIEDLNIKRTLFEQVEKFRKPGTLITSNTSGIPIHLMTEGRSDDFKKHFCGTH
                     FFNPPRYLPLLEIIPTQFTDKDVIDFISHFGDRYLGKTTVLCKDTPAFIANRIGVFGI
                     MSLFHSMEKTGLSVFAIDKLTGPIIGRPKSATFRTSDVVGLDTLVHVANDAYKNLPDD
                     EQKNLFAIPPFINKMIENKWLGDKTKQGFYKKTKTAEGKTEILELDLTTLEYKPSAKI
                     KFKTLESTKTVDDLKQRLPMLLSGTDEAASFYRYMFFSLFSYVSHRIPEIADEIYKID
                     DAMCAGFGWEIGPFETWDILGVKSVVEQMQKEGYSISPWVTEMISNGINSFYKIDNGK
                     KSYFDLQTKKLNQLPGEGSLVILKNTSNEKIVWKNSGCLLHDIGDGVLNLEFKTKMNT
                     IGSEVLQGINTAIDFAEKDFKGLVIGNEAANFSAGANLGLVFMLAVEQEYDELDYAVR
                     AFQNTNMRLRYSNVPVVVAPAGLTLGGGCEMSLHADKVQAAAETYIGLVELGVGLIPG
                     GGGTKELTLRFSDSIKPGDIETNALRDFFMTIATAKVSTSAFEAYDLHILRKGFDNIT
                     LNRKRLLSDAKNAVLSLANAGYVSPQPRHDIRVLGRQGLGVIQAGASAMLAGNYISQY
                     DQMLSLKLGYIMCGGNLSSPSLVSEKYLLDLEREAFLSLLGEKKTLERIQAILTTGKA
                     LRN"
     gene            complement(16882..17385)
                     /locus_tag="PK134_00225"
     CDS             complement(16882..17385)
                     /locus_tag="PK134_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRB37309.1"
                     /translation="MPKTIFLIRHSKATREYSQINDSDRPLTEKGCHDTEKMSLILKD
                     HINSVDLILTSTAVRAYASALIYSKHLGYHPNNIQLEKNLYMTGSKDMLAILKSLDDQ
                     INSVALIAHNPGLERLVKFLTGTESLHFSTSGLAMIESKIDAWNELNAGNCNLLSYRH
                     PKETIYN"
     gene            17566..18051
                     /locus_tag="PK134_00230"
     CDS             17566..18051
                     /locus_tag="PK134_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB37310.1"
                     /translation="MLKFLSIAQSKKRENKQVLKDLSKSKSPQLDNLFREESDNFFKK
                     FSCLDCANCCKTTSPIFRDVDIERIAAHLKLKPGDFVAKYLHLDSDSHYVLNHSPCPF
                     LGTDNYCSIYTVRPQACREYPHTDRKNINQIMDLTYQNTLVCPAVASIVEKLKTDFIK
                     K"
     gene            complement(18066..18416)
                     /locus_tag="PK134_00235"
     CDS             complement(18066..18416)
                     /locus_tag="PK134_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37311.1"
                     /translation="MKQKQKKSNNLSKVKNFDFDHYLNLRKAERIKLLQQMVEKLDPA
                     SRKNFFKSLLQYVLSDEELTALAFKNNSGKQEVKKSRVVELSDKEKEMLGQIADYFHE
                     LELIMDLDELLINP"
     gene            complement(18483..20264)
                     /locus_tag="PK134_00240"
     CDS             complement(18483..20264)
                     /locus_tag="PK134_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB37312.1"
                     /translation="MDTPVVKWIIRKLMLDSIYRSIQDDDILAKQRFNLFRIFNLVAI
                     IGIGLICYQAIYVFQTEKNLAYILLGLESLLILNYLLLNVHKNLTLSVVISLLSCFGA
                     LHYITYFAGGIRNSGMFYQIAFILISFMLLGSRYGIWFTILSVANMIYFFLITEYTNW
                     IDYSFIGETASLINQDYLITGLVSLIVISSMINNLESKKNVVISTITDSRNKLAIINK
                     ELKKLSLVASKTDNAVVITNENGVIEWVNDGFTRITGYQFYDAIDQSLQTFLSGPETD
                     KENLEAVQNSMREFKFCDSELLRYKKDGTTVWMHASITPISDNEKNSRKFIFIMSDIN
                     ERKKAEIQLSEYLSDLEKTNKELDKFAYIVSHDLKAPLRAIGNLTDWITQDSADSLSD
                     ESKENFQIIKGRVKRMEQLINAILEYSKASKHKGTQELFSFNEIVHNAVDLAGADSNC
                     KIEISGAMPEYYGDKVKYQQVFMNLISNGIKHNNKEQKHIEISCHEEGKFWEFKIKDN
                     GPGIDKRYHEKVFVIFQTLKARDEFESTGIGLSIVKKIVEEAGGSIRIESTPGEGSAF
                     FFTVPKNQIDASLITGKNNQQISPLVL"
     gene            20373..20798
                     /locus_tag="PK134_00245"
     CDS             20373..20798
                     /locus_tag="PK134_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB37313.1"
                     /translation="MNKQLTILLIDDDKVDSMIIKRAIAKLDFPYQVDTAHNGLDALM
                     MLRGMSDIRIKRPDIILLDINMPLMNGLEFLSELRADPELKDIHVCVLTTSEDDSDVK
                     TAYEFNVAGYFIKPLNISDFDDRFLYLNGYWQRNCFPVQ"
     gene            20947..22008
                     /locus_tag="PK134_00250"
     CDS             20947..22008
                     /locus_tag="PK134_00250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB37314.1"
                     /translation="MDSQTRYIPHLDGIRFIAAMMVVFNHYLSELNFDYKFSTGFYGV
                     QIFFAISGFLITGILLRLKNAEKVIPRRLIIKNFFAKRFLRLFPPYYLLIFSYIFLEC
                     FGLYIAQKGWIVFYLAYLPNWFFYFHGWQGTIDNHLWSLGVEEQFYLLWPFVLLLLPK
                     GTEFKWIITMIITGYLFKCVIRQYFMLGGDPFLLPISQMDTLGIGALLACGFEYRMQF
                     IEYFKKALPYLLPLAILLTLVTSYDKPRNDPFLCLSLILLSSSLVLNAVVGFKGVVKI
                     FMENKWVVYGGKISYGIYLYHKVIPTLFISSVERVGIGMPNRWLTLVVSIVLTFLISH
                     LSWKLIESPILKLKKNFEH"
     gene            complement(22005..23279)
                     /locus_tag="PK134_00255"
     CDS             complement(22005..23279)
                     /locus_tag="PK134_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB37315.1"
                     /translation="MENQEQKSKFQQAIDVITGVAKITIGAGIVHCKAQDEQMDGRYI
                     TLEGQKKLFFGTCGYLGIEKHPKIIEAAVEALHSHGAQFSSSRAYVSSHYYEEAESLF
                     SKMFGRPALVMQSLTLGHITGLPLLVGDNDAIIMDVQAHDSIQSATAMLKLRNVKIDI
                     VRHNRMDILEDRIKVLKNRYQKIWYLGDGVYSMHGDFAPVKELYALADKYEQLHLYLD
                     DIHGMSWTGPHGTGMVMNAVPYYHRKLFLSTGMTKAFGTAGGLLTYPDEEYFKLVKTC
                     GKGFIFSIQLPPAILAATIASCKIHMSDEIIPLQQSLMAKIDYFNKKAEELGLLVIRK
                     ERSPVFFIGVGNPNVGYSTVLRMKKAGFFFNLSVFPAVSIKHTGLRITLSNHIQIEDI
                     DELLETLAENLPVVFKENNTSFEQIKEEFANH"
     gene            complement(23358..24518)
                     /locus_tag="PK134_00260"
     CDS             complement(23358..24518)
                     /locus_tag="PK134_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRB37316.1"
                     /translation="MKQQVGIAGAGSMGSGIAQVAATNGNIVTLFDTNIEALQKAKAS
                     IRSSLDKLVEKNKIEKTLSDSIFSQITFTDNLNELKSSDLFIEAVIENIEIKQSLFKS
                     VESIVSDKCILATNTSSLPVIAIGSTCNQQDRIIGLHFFNPPVLMQLVEVIPSFNTSE
                     KVLTNSVEIIRQWSKTPVIAKDTPGFIVNRIARPYYGEALRIYEEGIADFATIDHAMK
                     TIGGFKMGPFELMDFIGNDVNFKVTETVFTQMFFDPRYKPSITQKRLYEGKLYGRKSN
                     RGYYDYKNNIQPKPSLDNNLHSQIFDRIFAMLVNEAADALMMGIATAVDIETAMTKGV
                     NYPQGLLTWANKRGLRAVYNKMEELFAYYGEDRYRVCPLLRKLALNDEMFRI"
     gene            complement(24515..25294)
                     /locus_tag="PK134_00265"
     CDS             complement(24515..25294)
                     /locus_tag="PK134_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRB37317.1"
                     /translation="MESTVLQEIKGQVVQLTLNRPEKFNSFNRVMSLQLQDELKKAAN
                     NSDIRAILITGTGKAFCAGQDLSEAVDTNGPGIAAIVREHYNPIITLIRNIEKPIVCA
                     VNGVAAGAGANIALCCDIVLAAESAVFIQAFSKIGLIPDSGGTFFLPRLIGFQRASAL
                     TMLGDKVSAAEAVSMGMIYKSVDDALLMETAMQISQTLANLPTKALGLTKKLLNQSLS
                     NNLNTQLECELEEQVNAAKSYDHSEGIKAFLEKRKPVFKGQ"
     gene            25409..26212
                     /locus_tag="PK134_00270"
     CDS             25409..26212
                     /locus_tag="PK134_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRB37318.1"
                     /translation="MESNFATPSNLVSDSVSAFWQVHRSKESALHETIIPKGVIEIIF
                     NFETTQLQAQVNNRHITVPRCFIQGYSTSPIHLHLDGSQTFFGVVLNPAAVKYIFNFY
                     PQIFANCVIDLTLIDSTFCFLWHKLGEQNNFNNRVQVFTDWLINRLPELNEREKAFNC
                     FLNSDSNIQLSVTQLANLFCYSTKQLSRKIFELAGMNTEQTLLYKKYLKAVHLIHSSD
                     LKLTEVAYLCHFCDQSHFIKTFKSLSYITPHEYVNRKSNIVGHIFENVH"
     gene            26272..26751
                     /locus_tag="PK134_00275"
     CDS             26272..26751
                     /locus_tag="PK134_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37319.1"
                     /translation="MTNYKNLIYALLCLSFSIIVGGSLFEHIAVWPNAFAAPPKSLTM
                     FQGEYAFNPEPFWKSIHPVTLILFIITLIISWNTTRKKNVLIAFMGYVVILIATFIYY
                     LPELKDLIATPFADLINQDLVQRGARWQTLSLFRTALAIILALILYFGLSKSDNKLS"
     gene            complement(26853..>27379)
                     /locus_tag="PK134_00280"
     CDS             complement(26853..>27379)
                     /locus_tag="PK134_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37320.1"
                     /translation="ANFAIKGVFVDSRQTCGNNIWTKAVFTCLYNLSDSCLGILIDCT
                     NTFNYANGLGMVSFDASTFYPVYTELIFYSKNGVSCGTPINFNQLLSVNEINKESFTL
                     LNNPTNGILRIQTTIPESIITIREPSGKEIWHKTIKNTGLVELPLNVANGIYLLTVEN
                     QNNRTTKKLIVNNY"
BASE COUNT         8999 a         4644 c         5126 g         8610 t
ORIGIN      
        1 gtgttgccct tacccgtttt atgaaacgtg aaggacaaac atggatacgt attttccctg
       61 acaagcctgt tacacgtaaa cctgcagagg ttcgtatggg taaaggtaaa ggagcaccgg
      121 aatattggat tgcccaagta aaaccaggaa ctatgctttt tgaagcagat ggtgtgtctg
      181 tagaaacagc caaagaagca atgcgtttgg cagcacaaaa attaccggta ataaccaagt
      241 ttgttattcg cagagattat tcagctgcct aacattaaaa tttgaaaaac gatgaaacag
      301 tcaatagtta aagatttaac aacagaagaa gtaaaatcac gcattaagga agaagtggat
      361 aatttacaga agcataaaat gcaacatgcc gtttctccaa tggataatcc gatgaaaatc
      421 cgcagcactc gcaaacttat tgcaaggctg aaaactgagt taaacaaaag aatgtcacaa
      481 acatcaaaat aaattattca atgagtactg aaagagcatt acgcaaaaca aaaataggtg
      541 tggttaccag cattaaaatg caaaagactg ttgttgtggc tgaagaaaag aagtttaaac
      601 atcctatgta cggaaagttt attaataaga ctaaaaagtt tcacgtacat gatgaaacta
      661 atgcatgtgg tagtggcgac cgtgttttaa ttatggaaac cagacctatc agcaaatcga
      721 agcgttggag attagttgaa attcttgaaa aagcaaaata attaaaattt ctcgccctac
      781 tcaaaggcag aatgtagaaa agaaataaaa gatgatacag tcagaatcaa gattaacagt
      841 agcagacaac agcggagcaa aagaagtttt gtgtatccgt gtgttgggcg gtacacgcaa
      901 aagatatgct tcgttgggag ataaaattgt ggtaaccgta aagaatgctt tacctgcagg
      961 aaatattaaa aaaggtgctg taagcaaagc tgtggttgtt agagtaagta aggaagtaag
     1021 acgtaatgat ggttcttata tccgtttcga tgataatgca gtggtgttac tcaatcaggc
     1081 agatgaactc agaggtacac gtattttcgg acctgtagct cgtgaactgc gcgaaaaaca
     1141 gtttatgaaa atcgtttcat tggcacctga agtaatttaa tttataaaat cagaatcatg
     1201 tcaaaactaa agataaaaaa aggcgacatc gttaaggtta ttgccggtga gtcaaaaggc
     1261 aaacaaggcc gtgtgcttga agtatttcct gaaacaaaca gggcaagtgt tgagaatgta
     1321 aatatcgttt caaaacatac acgtccgaat gcacaaaata cacaaggcgg tatcgtaaag
     1381 aaagaagctc cggttaatgt ttcaaatctt atgcttattg atcctaagtc aggaaagcca
     1441 acacgtgtag gtcgtaaaac agaagaaggt aaattagtac gttacgcaaa aaaatcaggg
     1501 gaggtaataa aataatgagt tacacaccgc gcctaaaagg caagtataaa aaagaaatca
     1561 tctctgcact gaaaaatcat tttcagtata agagtataat gcaggtacct aaattatcaa
     1621 agatttgtct tagccagggt attggcgatg cagttagtga taagaaaatt gttgatgctt
     1681 gtgtagcaga aatgacagct attgcaggac agaaagccgt agcaacttat gctaagaaag
     1741 atatttctaa ctttaaagta agaaaaggtg ttcctattgg agtaagagtg actttgcgtg
     1801 atgagcagat gtatgaattc cttgatcgtt tggtgtcagt atcgctgcca cgtattcgcg
     1861 acttcagagg aatcaataac aaaggatttg atggtaaagg aaattacaca cttggcatta
     1921 ccgagcatat cattttccct gaaatcaata ttgacaaagt aagtaagatt cgtggaatgg
     1981 atatcacatt cgtaactaca gctaaaactg ataatgaagc aatgcagtta cttaaagaat
     2041 tcggactacc attcagaacc ggttctaact aaaaaataat tcgaaaaaat ggcaaaagaa
     2101 tcaataaaag caagaagcag aaaaagagaa catttagttg aaaagtatgc agctaaacgt
     2161 gcagcattaa aagaagctgg cgattatcag gcattggcat tgttgcctcg taattcatca
     2221 cctgttcgtt tacataaccg ttgtatgatt acaggtcgtc caagaggtta tatgcgtcag
     2281 tttggaattt cacgcgtact gtttcgcaaa atggctttag aaggaaaaat tgccggagta
     2341 acaaaagcca gttggtaata ttaaacaata attagaaaaa taattaaaat gaccgatccg
     2401 atatcagatt atttgacacg tttgcgtaat gccgtaaagg ccaatcaccg catcgtagaa
     2461 gtacctgcat caaatactaa aaaagaaatt accaaaatac tttttgacaa agggtatatt
     2521 gcaaattata agtttgaaga aactgacaat aagcagggaa atattaaagt tgctttaaaa
     2581 tacaatcctg taaccaaatt gccggcaatt acgcgcctta agcgtgttag ccgtccaggt
     2641 ttgagaaagt atgttagtca tgatcgtttg ccaagaatta tcaatggtct tggagtagca
     2701 attatctcta caagcaaagg aattatgact gacaaagaag cacgtcagct tcatgttggt
     2761 ggtgaggttt tatgttatat ttattaattt aagaaagcat gtcacgaata gggaaattac
     2821 cggttgcagt accatcaggt gttcaggtta atgtacaagg aaatacagtt tctgtaaaag
     2881 gtccaaaagg caccttatca caaaccatag ccgatggcat tactttagaa atggaagaaa
     2941 ataaagttgt tgtaaaacgc agcacagagc aaaaacgtca taagtcatta catggcttgt
     3001 atcgttcatt gcttgccaat atgattaaag gagtatcgca aggctataaa atagagcagg
     3061 agttagtggg tgtaggttac cgtgccagca atcagggtca gatgcttgat ttggttcttg
     3121 gtcactcaca ccatgtggtt tttgaactgc cgacagaagt taaagtaagc acaaagacag
     3181 agcgtggtag caatcctatg atcatacttg aaagtcacga taaacaactt atcgggcagg
     3241 ttgctgctaa aataagatca ctccgtaagc ctgaaccata caaaggaaaa ggtatcaagt
     3301 ttgtgggcga agtattaaga agaaaagctg gaaaatcagc tgctaaaaaa taagtaaagt
     3361 aagaaaatga agaattcaaa agattcacgc aggcttaaaa tccgtaaacg tattcgcgga
     3421 aaaatcaaag gtactaatga gcagccaaga ttgtctgttt tcagaagtaa taagcagatt
     3481 tatgcacaga taattgatga tgttgcaggt cagacattgg ctgcagcttc atcagcagaa
     3541 aatggtgcag caaaaggaac taaggttgag atttcaactt cagttggcaa aatgattgct
     3601 gaaagagcaa cagccaaagg tattaccaaa gtaacattcg acagaggtgg atatttgttt
     3661 catggccgtg taaaagcatt ggccgatgga gcaagagaag gtggattaaa attctaaaac
     3721 gtaacaatga tagcaacagc aaataaaaga gttaaaccgg gcgaaatcga attaaaagac
     3781 aggctcgtag cagtaaacag agtaactaag gttactaaag gtggaagaac attttctttt
     3841 gcagccattg ttgtggttgg tgatgagaaa ggtgttgtag gttatggttt aggaaaagca
     3901 aaagaagtta ccgatgcaat tgccaaagct attgacgatg caaagaaaaa cttagtgaag
     3961 gttacagttc ataaaactac tgttcctcat gagcaagatg ctgctttcgg tggtgcaaaa
     4021 gtatttttaa agccagctgc tcccggtacc ggtgtaattg caggtggtgc catgcgtgca
     4081 gttcttgaaa gtgtaggcat acatgacgtg ttagcgaagt caaaaggatc ttctaatccg
     4141 cataacgtgg taaaagctac cattactgct ttagctatga tgcgtgatgc tgtagcagtg
     4201 gctaatcagc gtggtgtaga tttaaacaaa gtatttaacg ggtaatatta tactcatggg
     4261 aaaagtaatt atcaaacaag taaaaagcgg aatagatcgc gataaaaatc agaagctaac
     4321 actaaaagct ttaggtttaa gaaaagtata tcattctgta gaaaaagaaa acactgatca
     4381 gataaaagga atgatcgaaa aggttagaca tctggttgag gtaacagaaa tcaaataaga
     4441 aaagcgttat gaatttaagt aatttaaaac ctgccaaagg ttcaacaaag aaaagcaaaa
     4501 gaataggtcg tggtgaaggc tctggtcatg gtggaacatc tacacgtggt cataaaggag
     4561 cacaatcacg ctcaggatat tcacgcaaat ttggttttga aggtgggcag atgccacttc
     4621 agagaagggt tcctaaatca ggatttaaaa acttcttcag agttgaatac acaggcatta
     4681 atttagatgt gttgcagaag ttaaacgaaa aatttggttt aacagaaatt gatcatagca
     4741 ttctttgcga aaaaggatta atcgcaaaac gtgagttagt aaaaatactt ggacgtggcg
     4801 agttaaaagt aaaaatgaat gtaaaagcac atgcattttc agagactgca aaaaatgcta
     4861 ttgaagccgc tggaggcaca accaccattc aagaaaaata atgaaagaac tgattcaaac
     4921 attaaggaac atattcaaga ttgaagagct aagaacaaag cttctcaata ccttgttttt
     4981 tattgccatt taccgcttgg cctctcatat tgtgttgcct ggtgtagatc ctcagcaact
     5041 tggtaattta aagaaccagg cttcgcaggg tatatttggc ttattagata tgtttgccgg
     5101 aggtgccttt tcacaggcgt caattgttgc attgggaatt atgccctata tctcagcctc
     5161 aatcgttgtt cagattttag gaattgctgt tccttacttc cagaaaatgc agaaagaagg
     5221 agagagtgga cgtaatcgtt tgaatcaaat aacacgctgg cttacagttt tagttgtggc
     5281 tggtcaggca ccagggtatt tagtaaatct tagagcacaa gcacaatctg ctattgttgc
     5341 ctattcaaac cctgatgcaa tgtcaccaac gttgtggtgg attacttcta tcatcattct
     5401 tattgcaggc actttgtttg tgatgtggct tggtgaacgt attcaggaaa aaggaatcgg
     5461 aaatggtatt tcattactta tcatggttgg tattattgcc cgattaccat ttgccttacg
     5521 cgatgagttt ttatctcgac tcaatcagac aggtggagga ttaattatgt tcctagtcga
     5581 attagtagca ctgtttgctg tggtcgtgat aagtattatg ttggtacagg gtacacgtaa
     5641 aatacctgtg caatttgcta agaaaataat tggtaacaga caatatggtg gggtacgtca
     5701 gtacatacct ttaaaagtaa atgctgccgg tgtaatgcca atcatttttg cgcaggcaat
     5761 tatgtttatt cctgcaacag tagcacagtt ctttcctgaa tcttcttcca tgcagggtat
     5821 tgcacactca ttaacagatt tccattctgt atggtataat gttaccttcg caatactgat
     5881 tatattattc acctacttct acactgccat tacagttaat cctaatcaga tggctgatga
     5941 catgaagaaa aatggaggat ttattcccgg tattaaaccc ggaaaacaaa cagcatcatt
     6001 tattgatgat gttatgtcga aaattacttt gccgggctct atatttcttg cgatagttgc
     6061 catcttacct gcattcgcaa caatattagg taccagtacc aactttgctc aattttatgg
     6121 tggtacatca ttgttgattc ttgttggtgt aatactagat accttacaac agatagaaag
     6181 tcatttattg atgagacatt acgatggatt aatgaaatca ggacgtatta aaggacgcac
     6241 atccattaat gcacctgttt aaaatataaa attgtgcaga ttgaatatcg ctccaaagag
     6301 gagatagatt taattagagt tagttcttta cttgttggaa aaaccattgc agaagttgcc
     6361 aaacacctgc gagacggaat aactaccgga gagttagata agattgcaga agattttatc
     6421 cgctctcatg aagctgtgcc tgcttttaaa ggctatcatg gattcagtgg ttcgttatgt
     6481 atatcggtta atgccgaagt ggttcacgga attcccggag caagagtttt gcatcctgga
     6541 gatatagtgt cacttgattg cggtgtagtt aaaaacggat attatggtga ttcggcttac
     6601 acctttgcta tcggtgaagt agatcagcag gcagtaaaac tactgcaggt tacacgcgaa
     6661 tgcctttatc ttggaataga gaaagcagtt gccggtaaca gaatgggtga tgtaagcagc
     6721 aatatccaaa aacatgcaga ggcaaatgga tttggtgttg ttcgcgaatt ggtaggacat
     6781 ggcataggaa agaaacttca tgagaagccc gaagtaccaa attatggtaa ggcagggcaa
     6841 ggattgttgt tgaaaccggg gttggtaata gcaattgaac ctatgataaa catgggaaca
     6901 aagaatgtga agcaactcaa tgatggatgg acaattgtaa cagcagatca aaaacctgct
     6961 gcacattttg aacataccat tgcagtggat aatgtaaagg cagaaatact gtcttcattt
     7021 gaagagatag aaagaagtga aaaagagaat ttgaatttat ataaaataga ttataagcag
     7081 catattatag catagtaaga ttagattaat ggcaaagcag acatcaatag agcaagacgg
     7141 aacgattaca gaagcgttga gcaatgctat gttcagagtg gaactggaaa acggacatga
     7201 gataattgcc cacatttcag gaaagatgag aatgaactat attaaaattc tgcccggtga
     7261 taaagtaaag attgaaatgt caccgtatga tctgacaaaa ggaagaataa cttatcgtta
     7321 taaataactg aagtaacgaa tcagaataaa aattaaggag atgaaagtta aaccaagttt
     7381 aaagaaaaga agcgttgatt gtaaaatcgt tagacgcaac ggtaaattgt atgtaattaa
     7441 taaaaagaac ccacgcttta aacagcgtca gggataatta catcttaaaa ataattgtaa
     7501 caaagaaaat agttaatatc aaatggctcg aatagcaggt atagatttac caagaaacaa
     7561 aagaggtgaa ataggcctta cttatatttt tggtatagga cgttcaacag cacaacagat
     7621 tcttgatgag tgcggaattt cgtacgataa gaaagtagat cagtggaatg acgatgaact
     7681 gaataaaatc cgttcattca ttaatgacaa tctgaaaatt gaaggtgctt tacgttcaga
     7741 aacacagttg aacattaaac gtctgatgga tatcggtagc taccgtggtg ttcgtcatcg
     7801 tttaggattg cctgtgcgcg gacaaagaac aaaaaacaat tgccgcacac gtaaaggtaa
     7861 acgtaagaca gttgctaaca agaaaaaggt aactaaataa tcagattata ttgaatcctg
     7921 aaaatcagca ttcagaataa ataaaaagaa taatggctaa acaacaacaa gcatcagctg
     7981 caaaaacagc aaaaaagaag gtggtaaaag ttgaggcgac cggtgaggcg catatcaatg
     8041 caactttcaa taacattatt ataagcctta caaatctgca aggacaggtt atctcttggt
     8101 caagtgcagg taaaatgggt tttaaaggtt caaagaagaa tacaccttat gctgcacagc
     8161 ttgctgcggc tgactgtgca aaaacagctt tcgatttagg attaagaaaa gtgaaagtat
     8221 ttgtaaaagg tccgggagca ggtcgtgaat ctgcaatccg ctcactcaat gcttccggag
     8281 tagaagttag tgaaattctt gaccttactc caataccaca caatggatgt cgtcctccta
     8341 aaagaagaag gatataattt attatcacaa atcagtttaa aaattaaaat attagaataa
     8401 tacaatggca cgttacattg gaccaaaaac aaaaatagca cgtaagtttc gcgagcctat
     8461 attcggacct gacagatatt tcgaaaaaag aagttatcct cccggaatgc atggtgcagc
     8521 taaaaagcgc gcaaagcaaa gtgaatatgc aattcagtta gctgaaaagc aaaaggtgaa
     8581 atatacttac ggaatgttgg agcgtcagtt tgctcgtttc tacagtattg cacttcgcaa
     8641 agaaggcatt acaggcgaaa acttattgaa gcttattgaa tccagacttg ataatgttgt
     8701 tttccgttta ggaattgcac caacacgtgc tgctgcacgt cagttagttg gacattgcca
     8761 tattacagta aatggtaagg tgaacaatat ttcatcatac atcttacgtc cgggtgatgt
     8821 tgttggcgtt cgtgagcgtt caaagtcttt cgaagcaata acagattctc ttgcatctcg
     8881 ttctaataag tacggttggt tagagtggga caccacagct atgcaaggta aatttgtgac
     8941 tacacctgca cgcgaagaaa ttcctgagaa tattaaggag caacttatcg tggaattgta
     9001 ctctaaataa ttcttgctaa ccctgaacag taaaactcag taaataatta aacaaataaa
     9061 acacaaaata taaaatggcc atacttgcat ttcaacgtcc cgacaaagtg attatgaatc
     9121 attctaccga aacattcggt cagtttgaat tcagaccttt ggagccgggt tatggtatta
     9181 ctgttggtaa ttctctacgt agaatattgc tttcttcact cgaaggacat gcaatcacca
     9241 atattaaaat cagtggagtt gatcatgaat tttcaacaat aaaaggagtt gttgaagatg
     9301 taactgaaat tatcctaaac cttaagcagg ttcgttttaa aaagcaaatt gaaggtacag
     9361 actctgaaaa agttcaggtt gtagtaaacg gtaaaacaga atttactgcc ggtgatattg
     9421 caaaacatac ctctgctttt caggtattaa atcctgaatt ggttatttgc caaatggatc
     9481 cttctgtaaa attggttatt gatatgacca ttgacaaagg acgtggatat gtacctgcag
     9541 aagaaaataa atcaaacagc catccattag gaactgttgc aattgattct atttacactc
     9601 ctataaagaa tgtaaaatat gcagtagaga actatcgtgt ggaacaaaaa accgattacg
     9661 aaaaattggt acttgaaatc acaaccgatg gaagtattca tcctaaagaa gcattacgtg
     9721 aagcagcaga aattctgatt catcacttca tgcttttcag cgatgagaaa ataacactcg
     9781 atacaaagtc taagactccg gttgaagagt ttgatgagaa cacacttcac atgcgtcaat
     9841 tgctaaaatc gaaacttgtg gatatggatc tttctgtaag agcattgaac tgtctgaagg
     9901 cagccgatgt tgaaactttg ggtgatttgg tttcgttttc gaaaacagac ttgttgaagt
     9961 tccgtaactt cggaaagaaa tcacttacag agttagaaga attggttcgc agcaaaaacc
    10021 ttaccttcgg tatgaacata cagaagtata tgcttgataa agaataattg aatagtttaa
    10081 agattacgta aaatgcgtca cggaaaaaag ataaatcatt taggcagaac acatagccac
    10141 agagaagcat tactctctaa cttagcttca tcattgattc tgcataagag aataaacaca
    10201 actgttgcca aagcgaaggc acttagaaaa tatgttgaac cattaattac caagtcaaaa
    10261 tctgacagca cacattcgcg cagaacagtt tttagctatc tgcagaataa agaagcggtg
    10321 agtgagcttt tcagagaagt tgcaccaaaa gtaaacgagc gtccgggcgg atatacccgt
    10381 atcctgaaaa caggattcag acccggtgat gctgctgaaa cttgcatgat ggagttagtt
    10441 gatttcaatg agttgatgcc ttcatcaaaa gaaaaaggta cagctaagaa agctaagact
    10501 acacgcagag gccgtaagcc aaaagctaag gctgatgctg caggaagcga gacaacggaa
    10561 gcaacggaag aataaattca acagaaaaat taatctaaag gggatgtcgg tttcggcatc
    10621 ccttttttta tttttatgtt tacagtgatt ttctctgata gcacaaatat gtttgttcaa
    10681 aatacttcta taccttttgc tgatgcagga tattctgcat taattactga ttatttaaac
    10741 aacaatattt ttttaaaaga atatgcatca tttcaatatg atgaatcagg ttttaaagag
    10801 gcattacaga aacgagtaaa gtataaagcc aaccgtgaca ggctttgcga agtattagag
    10861 cagcagtatt cagaaacaat ttttcaaaca gattggaaga cggataaatc atattctgca
    10921 attaaatctt tgaaagaaga gaatactttt acagtcacta ctggtcacca actgaacatt
    10981 tttaccggtc cactttattt tatttataaa atacttaccg taattaagca ggcagaattt
    11041 ctggctcaga gaaatcccgg ttataaattt gtacctgttt attggatggc cagtgaagat
    11101 cacgaccttg cagaagtgaa tcatatagat attgaaacat cgaaaataga atggcaaaca
    11161 aatcaaaagg gtgctgttgg cagaatgact actgaaggaa tgtattctgt tgttgaagca
    11221 ttgaaaaatc agttaaataa gaatgataat tttcaggagt tagcaacgat acttgataca
    11281 gcatatttaa aaaatgcaac gcaggctaat gctatcagaa gccttgtaca ttctttgttt
    11341 tcaaaatatg gtttggtgat tatagatgca gatgataaaa gattcaagca acaagcaatt
    11401 aaattgtttg aagacgattt gttcaataac acatcattta attgttttaa aaatctttct
    11461 gattttgaaa aagcgtatgg attacaaatc catgcaagag aaattaattt gttttattta
    11521 gtcaatggag accgtagcag aataattaaa aatggcgaaa gatttgaagt tgcagattca
    11581 ggtttatctt tctcaagtga tgaattgaaa aagcatatca atgagtatcc tgaacgattt
    11641 agcccaaatg ttgtactacg gccattatat caggaaataa ttttaccaaa cattgcatat
    11701 actggcggcc ctgcagaagt acattactgg ttgcaattaa aatctatttt cgataagctg
    11761 aaagtttttt atcccatggt tgtgcttcgt aatggtatga tggtagtgaa tgcagatcaa
    11821 gctactgttt tgaatgaatc ggctttggaa tggaaggatt tgtttcatcc aacagaacaa
    11881 gttgtgaata ttgcattgaa aagcaagtat ggtgaagaac taagtctaaa ggaggaaaag
    11941 gataccttgc aaaggagcta tgaagaaatg gagcaacgtt ttaaagcaat tgatataaca
    12001 cttgagaaac atgttaaggc agagtggagg cgaacagaaa aacggttgat ggcttcagag
    12061 aaaaaacttt tacgtgcatt taagaaaaag caggttgatg aaacttgtaa aatcagaaat
    12121 acagtgaaac aattgttccc aaatggtaaa ttgcaagaaa gaaaagaaaa tgtgtttcct
    12181 tatttaagca agtatggatt gcagtttatt gatgatttgt ataaaatttg tgaaccacta
    12241 ccaacaaatt tcaaaattgc ggtaacagca gaaaaataaa aaaacttaaa gtaattcgta
    12301 aatacctgca gcaccctgac ctgtaccaac gcacatggtg accatgccat acttcatctt
    12361 tcttcttttc atttcattaa gaagttgaac actaagtttg gctcctgaac atcctaaggg
    12421 atgtcctaat gcaattgcac caccatttac attggtaatt tccggattta atcctaaggt
    12481 ccttataaca gccagcgatt gtgatgcaaa agcctcattg agttctatta attccatatc
    12541 attttgcttg agtcctgcgc gtttcagaac tgatggaata gcataaactg gtcctacgcc
    12601 cataatacgc ggttcaatgg cgctgacagc ataagaaaca aaacgtgcaa ttggttttac
    12661 gttaagcgtt ttgagcattt tttcagacac tacaagtaca aatgatgcac cgtcactggt
    12721 ttgagatgag ttaccggccg taacagttcc tttagcatca aagacaggct ttaatttatt
    12781 taatttttct attgtagtat cctgacgtgg tccttcatca gtatcaacag tacttttatg
    12841 tgtctgccgt ttttcattgg catcaagata ggtctcattg atttcaacgg gaacaatctg
    12901 ctctttaaac aaattatttt ttatggcatt aattgctctt tggtgtgatt gaaaggcaaa
    12961 ctgatcctga tcttcacgat taattttaaa ttcttttgca actgcttctg cagttaaccc
    13021 catgttccaa taccattcag gattatttgc cgccacctca taattaggaa caatcctcca
    13081 tccgccaaac ggaataggcg acatacattc aacaccaccg gcaataatac aatcagctag
    13141 gccgctatga atttttgcag aagcaatagc aattgattcg agccctgatg aacaatagcg
    13201 gttaatggtc ataccaggaa ctttaactgt attaagtccc atcaaagaaa tcattcttcc
    13261 gatattgagg ccttgttctg cttcgggtgt ggcattacct gcaattacat catcaattaa
    13321 ttctgtatca aaattcggaa ctgattgtat gagatgtttt ataacagtta ctgcaagatc
    13381 atcaggtctt gtatttttaa attttcctct tggtgctttg ccaacagcag ttctatatcc
    13441 ggctacgatg tatgcatcca tttgttcttg ctatttaata aattagtgtt ggtagcaggt
    13501 tctaattaca aaagaaattt atttcttttc cggtgttgaa ggtggtggtg agggtgtttt
    13561 taatttagcc cctggaggta atttcttttt atcatcagct gcttttttag ctgctgcagc
    13621 ctttttagcc ttttcatcag cttccttagc agctgccagt gcttttgcat cctcacccat
    13681 cttatattca acctgactga ttaatttacc ttttctgtca taaaccttcc acaaaccatt
    13741 tttgagattt actttatatg gacctgtatt tcgtatttca ccatcactga aatatgttgt
    13801 ccatgttcca ctttttttac catttaaata ttcaccttcc atcttagctt gcccattgta
    13861 ataatatctt ttgtattctc catcttttat tccattggca tactctatct cctccattat
    13921 ctttccatag ggataatatt tttttgctat cccatttctc acatcatcta aatattcaat
    13981 ttcctgattt aattcaccgg cttcattata atatttccaa acaccttgcc gctgtcctaa
    14041 ttcatctgtt ttgttgcgat gtttttctct tccgggcgca tatcttggtt tttcttcgcg
    14101 ttgagcaaaa gcaaaagccg gtaaaaggat taacaacaac aacagttgtt ttaatatttt
    14161 catcttttat gattggtatg gtaattcaaa tggctaaaat aaaatatttt taatcaaaac
    14221 atgaatgaaa ttcagagttt ttctacatgt ttttcattta taaacagctc atttaatttc
    14281 tgtttttcag aggaaaaaca aataatggcc ttaggtaaat tgttgaaaac ccatgaattt
    14341 taaaatgctg acacaaatga ttaaatatta attccttaac gctttacctg tagttaaaat
    14401 tgcctgaatg cgctcaagtg ttttcttttc gcctaataaa gaaagaaaag cttctctttc
    14461 caagtcaagc agatattttt cagatactaa agatggcgaa cttaaattgc caccacacat
    14521 aatgtatcca agctttaacg aaagcatttg atcgtattgg ctgatgtagt tgcctgccag
    14581 catagcactt gcaccggcct gtataacgcc aagcccttgt cttcctaaaa cacgaatatc
    14641 atgtcttggt tgcgggctca catatccggc attagctaaa gataaaactg catttttagc
    14701 atcagataaa agtctttttc ggttaagcgt aatgttgtca aaaccttttc taagaatatg
    14761 caagtcgtaa gcttcaaatg ccgaagttga aacttttgca gtggcaatgg tcataaaaaa
    14821 atcgcgtaat gcatttgttt caatatctcc gggtttaatg gagtccgaaa aacgaagtgt
    14881 caattctttg gtaccaccac ctccgggaat cagtccgaca cctaactcta caagaccaat
    14941 gtaagtttct gctgctgctt gcactttgtc tgcatgcaaa ctcatttcgc aaccacctcc
    15001 taaggtaagg ccggcaggtg caactacaac aggtacattg ctatagcgta aacgcatatt
    15061 ggtattttga aatgctctta cagcataatc aagttcatca tattcctgtt ctacggcaag
    15121 cataaaaacc aaacccagat ttgcacctgc agaaaaattt gctgcttcgt ttccaattac
    15181 taatccttta aaatcttttt ctgcaaaatc aattgcagta tttattcctt gtaatacttc
    15241 actacctatt gtattcattt tggttttgaa ttccagattc aaaacaccat caccaatatc
    15301 gtgtaacagg cagccggaat ttttccagac aatcttttcg tttgaagtgt ttttaagaat
    15361 tactaaactg ccttcgcccg gaagttgatt taatttttta gtctgtaaat cgaaatatga
    15421 ttttttgcca ttatcaattt tgtagaaaga atttattcca ttactaatca tttctgtcac
    15481 ccatggtgaa atgctataac cttctttttg catttgttct actacggatt ttactccaag
    15541 aatatcccat gtttcgaagg ggccgatttc ccaaccgaaa cctgcacaca tggcatcatc
    15601 aatcttgtaa atttcatctg caatttcagg aatgcgatgc gaaacgtagg agaataagga
    15661 gaaaaacatg taacgataaa atgatgctgc ttcatcagtc ccggagagca acataggaag
    15721 tctttgtttc agatcatcaa cagtttttgt actttctaat gttttaaatt ttattttagc
    15781 tgatggctta tattctaaag ttgttaaatc caattcaaga atttctgttt taccttctgc
    15841 agtttttgtt tttttataaa agccttgttt ggttttatca cccagccatt tattctcaat
    15901 cattttattg ataaatggag gaattgcaaa taaatttttc tgttcatcat caggcaaatt
    15961 tttataagca tcatttgcca catgtaccaa tgtgtctaat ccaactacat cacttgttct
    16021 aaaggttgca gactttggtc ttccaattat aggacctgta agtttatcaa tggcaaaaac
    16081 tgaaagacct gttttttcca ttgaatgaaa caatgacatg ataccaaata caccaattct
    16141 gttagcaata aatgcaggag tgtctttgca tagaacagtt gttttgccta agtagcgatc
    16201 accgaagtgg gaaataaaat caatcacatc cttatcggta aattgagtgg gaatgatttc
    16261 aagtagagga aggtaacgcg gagggttaaa gaagtgtgtg ccgcagaaat gttttttaaa
    16321 atcatcagag cgaccttctg tcatcaaatg aataggtatt ccggacgtgt ttgatgtgat
    16381 tagtgttccg ggctttctga atttttctac ttgttcgaat aaagttcgtt taatatttag
    16441 atcttcaata attacttcta taatccaatc gaagttagca atgtctttta gattatctgt
    16501 aaaattccct attgaaattc tttgtgcaaa gcttttatga tagagtggag aagggttact
    16561 ttttattgct gactgtaaat gagtatctgt aattttattt cgaaacttcg gactatcttc
    16621 aacaaaacct gcttttttat cagcttctgt aaattctttt ggtgcaatat ctaatagcag
    16681 tacattaaaa cctgcatttg caaaatgaca tgcaattcgt gaacccataa ttcctgaacc
    16741 cagaacagct actttttcaa tttttcttct cataaaaact atctaaaaaa taaaacccaa
    16801 acctaagtta ttttacgaat aaggtttgta aaaaccagat aaagttatga aaaacaacag
    16861 aactgttttg tcaattagta actaattgta aatagtttct ttaggatgtc tgtatgagag
    16921 taagttgcaa ttacctgcat tcagttcatt ccaggcatca atcttagatt caatcattgc
    16981 tagaccactg gttgaaaaat gcaaactctc tgtaccagtt aaaaatttca ccaaccgctc
    17041 taaacccgga ttgtgagcaa ttagagctac agagttaatt tggtcatcta agctttttaa
    17101 tatggcaagc atatcttttg aacctgtcat atataggttc ttttcaagtt gaatattgtt
    17161 aggatgatag ccaagatgtt tgctatagat taaagcagat gcataagctc tgactgctgt
    17221 acttgtcaaa attaaatcaa cggagtttat gtgatctttt aaaatcaaac tcatcttttc
    17281 agtatcgtga caaccttttt ctgttagcgg tctatcgctg tcattaattt gagaatactc
    17341 gcgagttgct ttgctgtggc gaattagaaa aattgttttg ggcatagttg gtagttaaat
    17401 ttttaaatag ggtgattaaa aaaaattaaa ttagttaaat catgcaacaa ttttaacaaa
    17461 aatgatgcct ttttgcaagt tattatttaa ttttttttga acgggtataa aacaagttaa
    17521 gaaaatgaca ctattttgtt tctttgcttg tgcatgaaac aggaaatgtt gaagttttta
    17581 tcaatagcgc aaagcaaaaa gcgtgaaaac aaacaggttt tgaaagacct ttccaagtct
    17641 aaatcgcctc agttagataa tttattcaga gaggaaagtg ataatttctt caagaaattc
    17701 agttgcctgg attgtgccaa ttgttgtaaa acaacaagcc caattttcag agatgttgat
    17761 attgaaagaa tcgcagcaca tttaaaatta aaaccgggag attttgtagc aaaataccta
    17821 catcttgatt ctgatagcca ttatgttctt aatcattcac catgtccttt tttaggaaca
    17881 gataattatt gttcaattta tacggtacgt ccgcaggcgt gtagggagta tccgcatact
    17941 gaccgtaaaa atatcaatca gataatggat ttaacttatc agaacacctt agtgtgtcct
    18001 gcagtggcgt caatagttga aaaattaaag acggatttta ttaagaaata gaaatgtaat
    18061 gattcttatg gattgatgag taattcatct aaatccatta tcaactccaa ctcatgaaaa
    18121 tagtcggcaa tttgtccaag catttctttt tctttatcag acaattcaac tacccttgat
    18181 ttttttactt cttgtttgcc actgttgttt ttaaatgcta aggcagtaag ttcttcatct
    18241 gacaaaacat attgaagtaa actcttaaag aaattttttc ttgaagcagg atcgagtttt
    18301 tcaaccattt gctgcaagag tttaatacgc tctgcttttc ttaagttaag ataatggtca
    18361 aagtcgaaat tttttacttt agaaaggtta ttagattttt tttgtttctg cttcattgct
    18421 atgaattttt atatttcata cgatgaatat gcaagcaggt tgcaaaaaga aaaacaaaaa
    18481 tcttacaaaa ctaaaggtga aatctgctga ttgttttttc ctgtaattaa ggatgcatca
    18541 atctggtttt ttggtacagt aaagaaaaaa gctgaacctt ctccgggagt ggattcaata
    18601 cgaatgctgc caccagcctc ttctacaatt tttttaacaa tagaaaggcc aataccggta
    18661 gattcaaatt catctcttgc ttttaatgtc tgaaatatta caaacacttt ttcatgatat
    18721 cgtttgtcaa ttcccgggcc attgtcttta atcttaaact cccaaaactt accttcttca
    18781 tgacaactga tttcaatatg cttttgctct ttgttattgt gtttaatacc attactgata
    18841 aggttcatga atacttgctg gtatttaact ttatcgccat aatattcagg cattgcacct
    18901 gaaatttcta ttttacagtt actgtcagca ccggctaaat caacagcatt atgtacaatt
    18961 tcattaaatg aaaatagctc ttgagtgcct ttatgttttg aagcttttga gtattccaaa
    19021 atggcattga taagttgttc cattcgtttt acccggcctt taataatctg gaaattttct
    19081 ttcgattcat ctgagaggct gtctgcactg tcttgggtaa tccagtcagt caggttaccg
    19141 atggcacgta atggtgcttt cagatcatgc gaaacaatat atgcaaactt gtcaagttct
    19201 ttatttgttt tttcaaggtc acttaagtat tcgcttaact gtatttctgc tttcttacgt
    19261 tcgttaatat cactcatgat aaaaatgaac ttgcgagaat ttttttcatt gtcgcttatt
    19321 ggtgtaatac tggcatgcat ccacactgtt gttccatctt ttttgtaacg caaaagttca
    19381 ctgtcacaga atttaaattc tcgcatactg ttctgcactg cctcaagatt ttccttgtcg
    19441 gtttcagggc cgcttaagaa ggtttgcagt gattgatcta ttgcatcata aaattgataa
    19501 cctgttattc gtgtaaaacc atcattgacc cattcaatga caccattttc attagtaata
    19561 acaacggcat tgtctgtctt gctggctact aatgaaagtt ttttaagttc tttattgatg
    19621 atagcaagtt tgtttcttga atctgtgatg gtagaaatga ctacattttt tttactctct
    19681 aaattgttga tcatgctgct gattacaatc aaactgacaa gccctgtaat caaataatcc
    19741 tgattgataa gagatgcagt ttcgccaatg aaactatagt caatccagtt agtgtattca
    19801 gttatcaaaa agaaataaat catgttggca acagacagta ttgtgaacca aatgccataa
    19861 cgactgccaa gtagcataaa tgaaatgagg ataaaagcaa tctgataaaa cattcctgag
    19921 ttacgaatgc caccggcaaa gtaggttatg taatgtaaag caccaaagca agataataga
    19981 ctaatgacta ctgatagtgt gaggttttta tgtacattaa gcagtaaata attcaaaata
    20041 agcagtgact ctaagcctaa tagaatatag gcaagattct tttcggtttg aaaaacataa
    20101 attgcctgat aacagattaa acctatacct atgatagcaa caaggttaaa gattctgaaa
    20161 aggttgaaac gctgcttagc taatatgtca tcatcctgaa tagaacgata aatactatcg
    20221 agcattaatt ttctgataat ccattttaca acaggtgtat ccataccggt ttgtgtagaa
    20281 aattgtgtta aacttaaggt gtttgtacgt tactgacaaa taaaatgttt tatttttgca
    20341 gataaatatt taggtgatat atttggcaat taatgaataa gcaattaacg atacttctta
    20401 ttgatgatga taaggttgac agcatgataa tcaagagggc aatcgctaaa cttgattttc
    20461 catatcaggt tgatacagcc cacaatggtc ttgatgctct aatgatgctg cgcgggatga
    20521 gtgatattag gattaaaaga cctgacatta ttttgttgga cattaatatg ccgttaatga
    20581 atggacttga atttttatct gaattacgtg ccgatccgga gctaaaagat attcatgtat
    20641 gtgttttaac cacttctgaa gatgattctg acgtaaagac tgcgtatgag tttaatgttg
    20701 ccggttactt tattaagcca ttgaatatta gtgactttga tgatcgtttt ttatatttaa
    20761 atggatattg gcaacgtaat tgctttccgg ttcaataagt ctctctttct ttttaaagac
    20821 atttgtttta gtgaccttgt aaattggttg aaaatctttc gactttcgac ctgaaatatc
    20881 ttgcattaac caaattctta ttgttgaaaa ctttagccga tatattttgg cgggctaatc
    20941 tcaacgatgg actcccaaac ccgttatata ccacatctgg atggaatacg ttttattgca
    21001 gctatgatgg tggtttttaa ccattatcta tccgaactaa attttgatta taaattctct
    21061 accggttttt atggtgtgca gatattcttt gctatctccg gatttctgat aacaggaatt
    21121 ttactaagac tgaaaaatgc tgagaaagta attccaaggc gacttattat caaaaacttt
    21181 tttgctaaac gtttcctcag gctttttcct ccgtattatc ttttaatatt cagttatatt
    21241 tttcttgagt gttttggatt gtacattgca cagaaaggtt ggattgtatt ttatttagcg
    21301 tatttaccca actggttttt ttattttcat ggctggcagg gaaccattga taatcacctt
    21361 tggtctttag gggtagaaga gcaattttat ttgttatggc catttgtatt attgctgctt
    21421 ccaaagggta ctgagtttaa atggataatc accatgatta ttaccggtta tctttttaaa
    21481 tgtgtcatca gacaatattt tatgttagga ggagatcctt ttttattgcc catttcacaa
    21541 atggatactc tgggtattgg agcattgctg gcatgtggtt ttgaatatcg aatgcagttt
    21601 atagaatatt tcaaaaaagc attaccttat ttgcttcctt tagcaatatt attaaccttg
    21661 gttacatctt atgataagcc acgtaatgat ccatttttat gtttaagcct gatattactt
    21721 tcaagtagcc ttgtattaaa tgctgtggta ggatttaagg gcgttgtaaa aatatttatg
    21781 gagaacaaat gggtggttta cggaggtaaa atcagctatg gaatttattt ataccacaag
    21841 gttattccta cattgtttat cagttcagtt gaaagagtag gaattggaat gccaaacagg
    21901 tggctgacat tggtagtgtc aatagttttg acttttttaa tttcgcactt gtcgtggaag
    21961 ttaattgagt cgcctatatt aaagctaaag aaaaactttg agcattaatg attggcaaac
    22021 tcttctttaa tttgctcgaa actggtgttg ttttctttaa acactacagg taagttttcc
    22081 gcaagggtct ctaacagttc atcaatatct tctatctgaa tatgattact taatgtaata
    22141 cgtaatcctg tgtgtttaat actaacagca gggaataccg ataggttgaa gaaaaagcct
    22201 gcttttttca ttcttaaaac agtactgtat ccaacattag gatttccaac tccaataaaa
    22261 aatacaggag atctttcttt tctgatgacc aataaaccta attcttcggc ttttttattg
    22321 aaatagtcaa tttttgccat caggctttgt tgcaaaggaa taatctcatc actcatgtgt
    22381 attttacacg aagcaatagt agcagcaaga attgcaggag gcaattgaat tgaaaaaata
    22441 aatcctttac cacatgtttt aactaattta aaatactctt catcaggata ggtaagtagt
    22501 ccaccggcag taccaaatgc cttagtcatg cctgtggaaa gaaacagttt gcgatgataa
    22561 tagggaactg cattcataac catacctgta ccgtgtggac ctgtccaact cattccatga
    22621 atatcgtcaa gataaaggtg caattgttcg tacttatctg caagtgcata aagctcttta
    22681 accggtgcaa aatcaccatg catggaataa actccgtcac ccaaatacca aattttctga
    22741 tatctgtttt tcagaacttt tattctatct tcaagaatat ccattctgtt atggcggaca
    22801 atgtctattt taacattacg caatttaagc attgctgtag cagattgaat gctatcatgt
    22861 gcctgaacat ccatgattat tgcatcatta tcaccaacaa gaaggggtag gcctgttata
    22921 tgaccaagtg taagcgattg cattaccaat gcaggacggc caaacatttt tgaaaacaga
    22981 ctttcagctt cttcgtagta gtgagatgat acataagcac gcgaagaaga aaattgtgcg
    23041 ccatgcgagt gaagtgcttc tactgctgct tcaataattt taggatgctt ttcaatgccc
    23101 agataaccac aagtgccaaa aaacaatttc ttttgacctt ctaaagtaat atagcggcca
    23161 tccatttgtt catcttgtgc tttacaatgc acaataccgg caccaatggt gatttttgca
    23221 acaccggtaa taacgtctat tgcctgttga aacttacttt tctgctcttg attttccatc
    23281 cctaacccaa aaaatgattc gcgtgcaaaa atagcgtttg taatagcaaa atactgatga
    23341 tatttatcaa attcagctta aattctgaac atttcatcgt tcaaagccaa ttttcttaaa
    23401 agtgggcata ctctataacg gtcttcgcca tagtaggcaa aaagttcttc cattttatta
    23461 taaaccgctc gcaacccacg tttatttgcc caagtaagca gcccttgagg gtaattaact
    23521 cctttagtca tggcagtttc aatgtccaca gcagttgcaa ttcccatcat caatgcatca
    23581 gcagcttcgt taaccaacat ggcaaaaatg cgatcaaaaa tctggctatg taggttgttg
    23641 tctaaagatg gtttaggctg aatgttattt ttataatcat aataccctct gttgctttta
    23701 cgtccataga gtttcccttc atataatcgt ttctgtgtaa tagaaggttt atatcgtggg
    23761 tcgaaaaaca tctgtgtaaa taccgtttca gtaactttaa aattgacatc gtttccaata
    23821 aaatccatca attcgaatgg ccccatttta aaaccaccaa tagttttcat tgcatgatca
    23881 attgtagcaa agtcggcaat accttcctca taaatccgaa gcgcttcgcc ataatagggg
    23941 cgggctatgc ggttaacgat aaaaccgggt gtatcttttg ctatcacagg tgttttactc
    24001 cattgtctga taatttcaac cgaattggtt aatacttttt cggaggtgtt aaatgacgga
    24061 attacttcaa ccaattgcat caaaacaggt gggttgaaaa aatgtaagcc tattattctg
    24121 tcttgttgat tgcatgtaga gccaatagca atgacaggca gtgatgatgt gttagtagct
    24181 aaaatacatt tgtcagaaac aatgctttca acacttttga ataggctctg tttgatttca
    24241 atattttcta taacagcttc aatgaaaagg tcagaacttt tcaattcatt taaattatca
    24301 gtaaatgtta tctgagagaa aatagaatcc gataatgtct tttcaatttt gtttttttca
    24361 accaatttat ccaaactgct tcggatagat gcttttgctt tttgtaaagc ttcaatgtta
    24421 gtgtcaaaaa gtgtaactat attgccattt gtagcagcaa cttgtgcaat gcccgacccc
    24481 atagagcctg ctcctgcaat tcctacctgt tgtttcattg tcctttaaaa acgggttttc
    24541 ttttttcgag gaatgctttt ataccctcgc tgtgatcata actttttgct gcattgactt
    24601 gttcttccaa ctcacattca agttgtgtat tcaagttatt gctaagcgat tgatttagta
    24661 atttttttgt aagtcctaat gctttggtag gtaaattggc taatgtttgt gatatctgca
    24721 tggcagtttc cattaacaat gcatcatcta ctgatttata tatcataccc attgaaacag
    24781 cttctgctgc tgaaacttta tcacccaaca tagttaatgc acttgctctt tgaaaaccaa
    24841 taagacgggg caagaagaat gtcccgccac tatcaggaat gagtccgatt ttactgaatg
    24901 cctgaataaa tactgctgac tctgccgcta atacaatatc acaacacaaa gcaatatttg
    24961 cacctgctcc tgcagcaaca ccattaacgg cacatactat aggcttttct atatttctga
    25021 ttagagtaat aataggattg taatgctcac gtactattgc ggcaatgccc ggaccgttgg
    25081 tgtcaacagc ttctgataaa tcttgacctg cacaaaaagc cttgcctgtt cctgtaatca
    25141 aaattgctcg gatgtctgaa ttgtttgcag ctttttttag ttcgtcctgc aattgcaaag
    25201 acattactcg gttaaaactg ttgaattttt ccggacgatt taaggttaat tgaacaacct
    25261 gtccttttat ttcctgtaat actgttgatt ccataattgg caaaaataaa tagttatcgg
    25321 aataatcagt aaacaataat tgatgattca ctattgcaga ataaagtttt ttgtttactt
    25381 tagagttctc ttttagcaga attgatatat ggagagcaat tttgccacac catcaaattt
    25441 agtttcagat tctgtatctg cgttttggca ggtacataga tctaaagagt ctgcattaca
    25501 tgaaactatc atccccaaag gtgtaataga aatcattttc aattttgaaa ccacacagct
    25561 acaggcacag gtaaataata gacatataac agtaccaaga tgttttatac aaggatatag
    25621 tacttcgcct atacatcttc accttgatgg aagtcaaact ttttttggtg ttgttttgaa
    25681 tccggcagca gttaagtaca tttttaattt ctatccgcaa atatttgcaa actgtgttat
    25741 tgacttgaca ctaattgata gtacattttg ttttttatgg cataaacttg gcgaacaaaa
    25801 caattttaat aaccgggtgc aagtttttac agattggtta ataaacagac ttccggagct
    25861 caacgaaaga gaaaaggcat tcaattgctt tttaaattca gactctaaca ttcaattatc
    25921 tgtaacacaa ttggctaatt tattctgtta ttcaaccaaa cagctttcca gaaaaatatt
    25981 tgaacttgca ggtatgaata cagaacaaac tttactttat aaaaagtatt tgaaagcagt
    26041 ccatcttatc cattcatcag atttaaagtt gactgaagtg gcatatttat gtcatttttg
    26101 tgatcaatct cattttataa aaacgttcaa aagtttgagt tacattacac cacatgaata
    26161 tgtgaataga aaaagcaata tcgttggtca tatttttgag aatgtccatt aaatacaatt
    26221 tagtcttttt ttctaatttc aaatttgttc cgcatattta atcctaacaa aatgacaaac
    26281 tataaaaatt tgatttatgc gctactgtgc ttaagttttt ctataattgt tggaggatca
    26341 ttatttgagc acattgctgt atggccaaat gcatttgctg cgcctccaaa atcattaaca
    26401 atgtttcagg gtgaatatgc ttttaatccg gaacctttct ggaagagtat tcatccggtg
    26461 acattgattt tattcattat caccttaatt atatcatgga ataccacgcg aaagaaaaat
    26521 gtgctgattg cttttatggg atatgtggtg attctgatag caacatttat ttactatctt
    26581 ccggaactta aggatttaat agccacacca tttgctgatt taataaatca ggatttagta
    26641 caacgaggtg ctcgctggca gacattaagt ttatttcgga ctgcactagc aattatttta
    26701 gctttgatat tgtattttgg attatcaaaa tctgataata aattaagtta aagttataga
    26761 atttcgattc aatagcttat agaggttata aattaaagaa caatttttaa atagaaaaaa
    26821 gtagctacat acacttgatg ataaaacgaa atttaatagt tgttaacgat taacttttta
    26881 gttgttctgt tattttgatt ctcaacggtg agtaagtaaa ttccgtttgc aacatttaaa
    26941 ggtaattcaa ctaaccctgt gttttttata gttttatgcc aaatctcttt tcctgaaggt
    27001 tctctaattg taattataga ttccggtatg gttgtttgta tgcgtaaaat tccatttgtt
    27061 ggattgttta ataatgtaaa actttccttg ttaatttcat taacagaaag taattggtta
    27121 aaatttattg gagtgccaca tgaaacacca ttttttgagt aaaatattaa ctccgtatag
    27181 accgggtaaa aagtagaagc atcaaaacta accataccta aaccatttgc gtaattgaaa
    27241 gtattagtac aatctatgag aatacccaaa caagagtcac ttaaattata aagacaagta
    27301 aaaacagctt ttgtccagat attattgcca caagtctgtc ttgaatccac aaaaactccc
    27361 ttaatagcaa aattagctg
//
LOCUS       DAORQT010000003        26423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_91_length_26423_cov_16.797520, whole genome shotgun sequence.
ACCESSION   DAORQT010000003 DAORQT010000000
VERSION     DAORQT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26423
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_26423_cov_16.797520"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PK134_00285"
     CDS             complement(<1..212)
                     /locus_tag="PK134_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37321.1"
                     /translation="MNKILFLIPAYSFKKLLKHIDLKLLRSYWIGFALSLTTLTSVAQ
                     NKVFTGGASYYAKSFEGRKTANGELY"
     gene            292..1365
                     /locus_tag="PK134_00290"
     CDS             292..1365
                     /locus_tag="PK134_00290"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRB37322.1"
                     /translation="MSTVKFNNKDAVFFPELKQRINQYFEQNKIKPTGNLKLYSKTII
                     LFTILIALYIILVFFTPENGWLSLFLASLLGLVMASIGFNVMHDGAHGSYSSHKWVNE
                     SMAHSLNLLGGVSFIWKQKHNINHHSYTNVEGMDDDIDIKPFIRVHEGQKRYWFHRYQ
                     HIYGLLLYGITYFFWVFYNDFNKYFSGKIAEHTHMQKMSIWDHVIFWATKIFYVLVFI
                     VFPIYNVGFIDTLLGYGVMVAVTGITIAVVFQLAHVVEDMPFVSPEGDVMKIESEWAV
                     HQLQTTTNFATKNKFISWLLGGLNFQVEHHLFPRISHIHYPSISKIVRETCLEYNVQY
                     KEFRTMRGALASHILHLREMGRK"
     gene            1621..4800
                     /locus_tag="PK134_00295"
     CDS             1621..4800
                     /locus_tag="PK134_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRB37323.1"
                     /translation="MKKTITLFICLFSLSLATWAQDKTVTGKVTSSQDKLGIPGVSIL
                     EPGTTNGTVSDIDGKYSIKVKSTTTQLRFSGTGLTTRTVDIPASNSLDLTMQPDVQKI
                     GEVVVTALGIKREEKSLGYSTQKVSGDDLAAAKEANVINSLQGRVAGVSITGSSNMGG
                     SSRILLRGMRSINFENQPLFVVDGIPMNNENVATVDQQRGALGYDYGNAIQDINPDDI
                     ESINVLKGPSATALYGSRGSNGVIIITTKKGVAREKSEKYSPIGVTLSTGWSMKKIYN
                     LPKYQNRYGGGASSDFIESDIHPGELRSEFEYDGSWGPELLGQEVYQWDSYVPSMANY
                     NKKTPWVAHPNNVKDFFETGIVKNNSVSLDGGNEKSLFRLGYTNYDEKGVIPNESLNR
                     NIISFNGSNKFNDRLSATLNMNYVVARGKGRAATGYNSIASNFNQWWQRQLDIKELKD
                     YKNPDGSQRAWNMNAEDDPSPLYWDNPYWVSYENYETDQRNRLYGGLTFDYDIGKGFS
                     IAVSGKTDYYNDNRQERVAVGSAVATAIPAYSENSITFNENNYDMILSYKHRLGDDLD
                     FNGFVGVNRVDQKFSNKFVSTQGGLNVPNFYNINNSTSSELIYSSQVRQLRKNSLFAS
                     ASFGYKSFLYLDVTGRNDWSSTLPEGKNSYFYPSITGSFVFSEKLDFNWLSYGKLRAG
                     WAQTAIDPVPYRSTLTSPSMSDNFNGLPTSVIPGTFNNTDLRPEMTSGFETGLELHFL
                     QKRISLDVAYSSTRSKDVIFNVQQSGASGIDFKIYNAAELTNKTVELMINVIPVKLKN
                     GLEWGVGMNWAKTNNKVVKLFTDELGNETENVQIGTAPFSATLQMRKGMEASQIVGYD
                     FVYDDKGNKVVGADGFYLRSETVKPLGSVLPDYTGGFNTYVSFKGIRLSGLIDFQKGG
                     KVFSLTNVWGKYSGIFAETAEGDIRENGLVNEGVTETGEQNTTVIAAVDHYFQDGGYV
                     ITAADVYDASFVKLREMTLSYELPQSLFKKAIRGVSVGVYGRNLAILHKNVPNIDPEA
                     GLSSGNVQGFEGGQLPTARIFGVNLNVRF"
     gene            4819..6345
                     /locus_tag="PK134_00300"
     CDS             4819..6345
                     /locus_tag="PK134_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRB37324.1"
                     /translation="MIKFKINQKAIATVMVFSLLLGSCKKNFESINEDPNNPKEVPNA
                     YLLTGAMRGLMDNTIDVWWGGNVGNQLGQYWSSNQYSSESRYLYRSNVTNSYWRYFYG
                     GVQNDQLVAVGGLYELSQIVKKCKEDSIEASRDGYVPNQIAVATIIRVWTFQNMTDIW
                     GDIPYSEALQADGNKTPKYDKQEDIYNGLLSEIDDAIGMINDAESGPNGDVVYNGDMT
                     AWKKFANSLKMRLALRIVDRNAGKAQSEFEAAAAGAFTSNSDNALLQYGDFPSGNPIY
                     YNRYISGRNDYASSNTFLDSTLTPLNDPRRECFFVPATATGLWTGEVYGLSEANAAQT
                     PNSRVAQRSPLVLSASLPGIFMDYAQVEFMLAEAAERGWAVTGSAESHYDAGITASIS
                     FWTALNGAAASAGDIAAYIAQPTVNYQTAGGTWQQRIGVQKWIALFDQGIQGWVEWRR
                     LDFNKLMMPADGVIDGTGIPLRMKYPVLEQTLNSASYSAAVGAQGADEQDTRMWWDVN
                     "
     gene            6456..7841
                     /gene="asnS"
                     /locus_tag="PK134_00305"
     CDS             6456..7841
                     /gene="asnS"
                     /locus_tag="PK134_00305"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRB37325.1"
                     /translation="MSHSYIKALLNTEPTGQIVKAKGWVRTKRGNKAISFIALNDGST
                     IHNIQVVAENNNFDESLLKNITTGACIAVEGKLVASQGQGQKVEMQATQIEIYGTADP
                     ETFPLQKKGHTMEFLRDIAHLRPRTNTFGAVFRIRHTMAYAINHYFHTNGFFYIHTPI
                     ITGSDAEGAGEMFRVTTLDMNKPPRNEDGSIDYSQDFFAKETNLTVSGQLEGELAAMA
                     LGKIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFFDLNDNMDLAEDMLKYLVRYALDN
                     CMDDLEFLNNMYDKELIGRLRSVIENPFKRLTYTEAINVLEPNNSKFEYKVSWGADLQ
                     KEHENFLVEHFKTPVILTDYPASIKAFYMKQNDDGKTVRAMDILFPWIGEIIGGSQRE
                     ENYDKLVSRMKAMHIPEKEMWWYLDTRKFGACPHAGFGLGFERLMLFVTGMNNIRDVI
                     PFPRTPRNAEF"
     gene            complement(7852..9075)
                     /locus_tag="PK134_00310"
     CDS             complement(7852..9075)
                     /locus_tag="PK134_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB37326.1"
                     /translation="MTKTRNAFVAWKHKDYRLFLIAKVCLTIAIQMQSIILSWLIYEM
                     TKDPLSLGIIGLIEAIPALSVALFGGHLADRLSRRKLLIWSCAITLSASAFLAIYVYD
                     FGKISTWPIYLTIFLIGLARGFHAPTQAAFWGQLVPKEHYVNASTWNSSLWQVAAVIG
                     PAMGGFFYGWIGVFYSSLVVCAFIIVTMICYLLIKDRHVDYHNKTESILQSLKTGVNF
                     VFKNQFIISAISLDLFAVLFGGAVALLPVFADQVLHVGAQGLGLLRAAPAVGSVVMAI
                     FLAFHPPRKDAGYKMLASVVGFGLCMIGFALSGSFLLSLFLLFLSGVFDNVSVVIRST
                     IIQSFTPDEMKGRVSAVNSIFVGSSNELGAFESGVMAKLMGLIPSVLFGGGMTLLVVL
                     ITFIKAKKLKELNLP"
     gene            complement(9134..9355)
                     /locus_tag="PK134_00315"
     CDS             complement(9134..9355)
                     /locus_tag="PK134_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRB37327.1"
                     /translation="MYWTLELASHLEDAPWPATKDELIDYAIRSGAPVEVVENLQELE
                     DEGESYESIEEIWPDYPTKDDFFFNEDEY"
     gene            complement(9469..10020)
                     /locus_tag="PK134_00320"
     CDS             complement(9469..10020)
                     /locus_tag="PK134_00320"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRB37328.1"
                     /translation="MKIYTKTGDSGKTSLIGGTRVSKHHLRIDAYGTVDELNSTIGLL
                     KDLAEEKDLCKEILAEIQDRLFTIGSSLAADPEKSKMKIPDLNTEDITLLEKEIDRMV
                     AALPPLKSFVLPGGHLLNSHTHVARCICRRAERLATGLSESEWVEPMVLIYLNRLSDY
                     LFMLARHVCFVRNITETPWRPRV"
     gene            10245..11825
                     /locus_tag="PK134_00325"
     CDS             10245..11825
                     /locus_tag="PK134_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRB37329.1"
                     /translation="MFKKVGLVLLLLAYCRSTSAQNIEIQKLGALLQMIDYYYVDTVN
                     KPKLVDDGIRAILKDCDPHSQYIPASELKEMNEPLVGKFEGVGIQFNILDDTIMVTQT
                     IAGGPSEKLGIRPGDRIVNIDGKKVAGVKITNKDVISKLRGDKGTKVKVDMYRRGEAE
                     LLTFDITRDKIPLFSVDASYKIEPDIGYIKISRFADNTVEEFKEALAKLKSQGVKSLI
                     LDLTSNGGGYLNRAHQLADEFLSDGKRIVYTEGRSQSREDYFSTAAGGWEKGKLVVMI
                     NEYSASASEIVSGAIQDWDRGLIVGRRSFGKGLVQKPYSLPDGSAVRLTVARYYTPSG
                     RCIQKSYQEGDEEYSDDISNRFKHGELYHADSIKFNDSLKYTTNAKRVVYGGGGIMPD
                     VFVPLDTMFSSKYYSDLIRKAALSEFALTYADNNRSKLKSAYTTVENFKKNFNVDDKM
                     MNDFIAFAEKKEVKKDEEGLKRSGTMIKAQIKALIARDIFNFGAYFYIINDSDDTFNK
                     AVESIKDNTFSKMKIANN"
     gene            complement(11831..14575)
                     /locus_tag="PK134_00330"
     CDS             complement(11831..14575)
                     /locus_tag="PK134_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37330.1"
                     /translation="MKKLILSFFFLTIIQQAKAENNQMPISAFVIQWTSAWCGSNSGA
                     LIAQVNGGVAPYSFFWNGIPGTDTLLGVPAGIYDLLVIDAQGDSAQSSGMVNAQVEVP
                     FSVISIAMSNPGSNNGAFQVVIYPGPSANDIPQGTYNYNLYDSTALNLLATHTFSYYV
                     GGAIDPYYIFDSLPAGKYYVHGVAPFGCYTQVEFRIKELPTINPTFSVSDACNGSPTG
                     NLSINLHPYPAINNNTFNSNLISSYTGAGNAVTSKYKTIITDSVNNTVGYRAVQDSLL
                     DYKNLLPGNYTLKVFTGDTIYSQGIPNIQDSILIYSTPFTIVNDPNCSLVKGRVFGDY
                     NSNCSYGFGDILFSGTLVELNPGGYSSVTNAAGEFFIPVVNGTYTLKQYAPYQFAQVC
                     PDTTTFTINITTPGTLVDVQVADSVNVNPDLQVGISGNQPRPGFNHHYKIDVRNLTPK
                     IIPAQTLTVSYDTTLNFVNAVPAPATNSNGVLMFTGISLPPFGTASFQINTVVPSTTP
                     LGTVLNASALITTVVGETIISNNIDSISQTVAGSFDPNDKAVMPHGFDAAGYITAAQQ
                     LKYTIRFQNTGTDTAFAVMIADTLPLSLNKYSLSVLNTSHPFTYEFASGNVVKFHFDN
                     ILLPDSNSNEPASHGYIKFKIDQIPGNAAGTVIQNNAAIYFDFNSPVITNTVTNTVFD
                     CNQMATVTLSNSTICENDSIQANSALLFDMNSEWLLDGTSYSNDSSFYLTGLSAGNYS
                     LTFVASNLVCSQSIINNIIVNPIPPQPFITNIANTLQSSSVSGNQWLLSGLPIGGATA
                     TSYIPTATGFYSVMVTDVNGCTKISDSIYILITSINNIGSANICIYPNPVKDELIISG
                     LTNGENVLTVFDAVGKVVLSVITSNTKEKIKTTFTKGIYFISIKNALHQIQMLRFTKE
                     "
     gene            complement(14894..15313)
                     /locus_tag="PK134_00335"
     CDS             complement(14894..15313)
                     /locus_tag="PK134_00335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB37331.1"
                     /translation="MTTKNIQTIQIESKPESIHIIERLVDDLKGEHNIHEDAYGNILV
                     AVTEAVNNAIQHGNKYNPNLKVKISYEVESDTISFAITDQGKGFDYYNLPDPTAPENL
                     EKPTGRGVFLMKHLADQIIFSNNGSSVELFFKTGHVA"
     gene            complement(15319..18657)
                     /locus_tag="PK134_00340"
     CDS             complement(15319..18657)
                     /locus_tag="PK134_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37332.1"
                     /translation="MTGNYRLLIEKLDEFIRKYYRNQVIRGSIYATGITIGFFLFISL
                     AEYFAWMGSSLRTALFYIYVFTVLAVIIRLIVIPLFKIWKLGKTLSYEQAATIIGNHF
                     TDVKDKLLNTLQLNELSENNHNELVQAGIDQKIQNLKPIPFTGAIDFKKNRKYLPLAL
                     IPAGTLIILLFAAPNVIKESTSRLVNYNVAYKKPAPFNFILLNKNFNVIEQNDFTLNF
                     KVEGQELPDQVYVTYEGRQIKADKEDKQHFTFTFHRIIKSTSFQLMADGFYSENYELK
                     SVPNPLISAFKIDLNYPAYLQKKNETLNNTGDLLIPAGTIVKWTFNTVNTSVFKLWFN
                     DSLTPLVSDNNQYTYSKRMLSGANYSIATANNFITNKDTLEYSINVIPDQYPIIQVEQ
                     QNDSSSLQRIFFKGTVKDDYGFSKLAFHYRFVKSKNDSSAHSDKLNTEILTINKNNNQ
                     TDFYHFWDGALHGVVPGDEVEYYFEVWDNDGVNGPKSAKSQSLIFKAPTLRELAENTE
                     KSNDEIKKDLQESLKQASKIQKQITDAARKLTEKKKTDFEDRKRIEDLLKQQKELDEK
                     LDKIKNEQQQNLAKQNEFKNNESQLAEKQQQIQELFDRLQNPELKELMKQLEQMLSQL
                     DKQSVQEALDKMKLNNKDLEKQMDRTLELFKQLEVEQKLKNTLENLDKLAEDQKKLSE
                     QSMDKNSDTEKLKEQQKDLNKQFEDIKKDIAALEKKNQELEFPQELANTEQEQKDIDQ
                     EQQNSEQQLNENKKSNAGKSQKNAAEKMQNMSQKMKNQMAQSESEQSEEDMASLRYLL
                     ENLLRFSFEQEALMQALKTTDVNNPQYVKMGQRQKKLQDDLKIIEDSLFALSKRVIQI
                     QQTVNTEISNIQSNIQKSIAFLQDRQVPVARSSQQYIMTSANNLALLLSEALQQMQMQ
                     QQQQKQGNGSCKKPGKGKPGPSAEKLKMMQQQLSEQMKKMKGKLEGMQKNGGKGDKGM
                     SEELARMAAQQEAIRNELNQLNQQENKDGKNSMGNLGNLANQMEQNERDIVNKKITQE
                     TLKRQQEILTRLLEAENAQREREQDQTRKAEQAKEQFLRNPAAFEEYKKLKEKQTELL
                     NTVPPGLNTFYKNLVNNYFQGLQF"
     gene            complement(18829..20166)
                     /gene="accC"
                     /locus_tag="PK134_00345"
     CDS             complement(18829..20166)
                     /gene="accC"
                     /locus_tag="PK134_00345"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRB37333.1"
                     /translation="MFKKILIANRGEIALRIIRTCKEMDIRTVAVYSTADRESLHVRF
                     ADEAVCIGPPPSKDSYLNIPNIIAAAEITNSDAIHPGYGFLSENSKFSAICAKNKIKF
                     IGATPEQIDGMGDKSNAKDTMKKAGVPTIPGSEGLLTSVEEGLKIAAEIKYPVILKAT
                     AGGGGRGMRIVWKDEEFAAAWDSARQEAGAAFGNDGMYLEKYIEEPRHIEIQIAGDQY
                     GKACHLSERDCSVQRRHQKLVEETPSPFMTKELREKMGEAAIKAALAVKYEGVGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPVTEEVINYDLIKEQIKIASGEPISGKNYYPTMHAIE
                     CRINAEDPYNNFRPSPGRITNLHVPGGHGVRVDSHVYAGYTIPSNYDSMIAKLITIAQ
                     TREEAINTMERALSEYVIEGVKTTIPFHIQLMKNEDFRKGNYTTKFLESFKLL"
     gene            complement(20241..20726)
                     /gene="accB"
                     /locus_tag="PK134_00350"
     CDS             complement(20241..20726)
                     /gene="accB"
                     /locus_tag="PK134_00350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRB37334.1"
                     /translation="MNKKEIEELIRFVAKAGVSEVHLEQKDFKITIKNPTKEQIVHVA
                     PAVQPVMQMPAPAQPQQVIAAPAVAAAPAAKTAGDDSKYKTIKSPMIGTFYRTPSPDK
                     PNFVNVGDEVKEGQVICIIEAMKLFNEIESDIKGKIVKVLVEHSTPVEYDQPLFLIDP
                     S"
     gene            complement(20913..25991)
                     /locus_tag="PK134_00355"
     CDS             complement(20913..25991)
                     /locus_tag="PK134_00355"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37335.1"
                     /translation="MEKQIIPQKLFSALKVVFVFIALFFFKGAIAQTQTFTSSGTFTV
                     PAGVTTVQVEAWGGGGAGGGVASGIFPPTRAGGGGGGGAYAKNSSVTVIPGSTITVTV
                     GAGGAGVGGADGNNGGSSSFGGLVTVSGGLGGKHGSTSPTNGAGGNGAFGTRNGGNGA
                     AASGGNSGGGGGGAGTSGNGGNASGMTGGTGGSGGGGNGANGRSSNGDGADASALSGG
                     GAGGRNSSSQWGSRTGGDGYRGQVIVTWVCPAYNITAISAVTPICPLPGTSVISLTGS
                     VTDLPVGSYTVTYNRSLPAAAGLTTTMTVTTAGSGTFTATGLTTSGSPVITVTNLSSG
                     APGGICSSVISTNNSVSVTVNAAPSVSASNDGAVCAGQTIQLHAVASGVTFQWSGPSG
                     FNSSLQDPSISGAAAANAGIYTVTATDPLAGCSATATTNVVVNALPTATASSNSPVCV
                     GFTINLSTPSVSGASYAWTGPDGFTSSIRNPSISNAQIVNDGSYTVVVTSSAGCTNTS
                     SVTVAVNNAPIASASSNAPVCTATTLNLNASPSGATYAWSGPAGYTSSTQNPTRSNAT
                     LAMNGTYTVTVTLGGCSSTATTTVVVNQAPTGTPTVSNATVCEGSAVTLNANQGLPVS
                     YSASPNVSIPDNNVSGVNSTITIPAQTIASATQLKIGINLTHTYSGDVIAKVTSPGGV
                     ITVFDRLGVPASSFGNGQNFGGYYEFDVSAGSVLPESNSGPTTIPAGTYAPSNTNGSI
                     NTNWSGISFPMNAGGNWVLNMSDRASGDVGTIVSWTVTIMTSSGITYTWTSTPNGFNS
                     TDENPSATVTGTTTYNLAATANGCTANTTVGVSATPGPLAIASSNAPVCDGNDINFIA
                     DNLAGGQGSGNSYQWYDPNNLPFSSQQNPNIFSATSAYNGTYTVVVTNQYLCTATTTV
                     QVDVNPNPVINVSTQINVNCFGESNGSITTNTSNGTGPYSYTYDFVNFNTTGEFDLLP
                     AGSYTIYVSDANACQNTVTATITEPALLQFTKTYTDQQCPSTFGSITMNATGGTTPYM
                     YSYDNGSTYVNNNANLNLTAGSYNVMVQDAHMCATSTQQQDLILVNTPSTAALSVSSQ
                     NGTELCIGGSTTLSAVGGSLGSTADWKWYKTNCGTTPVGSGPSITVNPSSSTSYYVRA
                     EDVCGATPCADLSLAITVGVPSAAVTVPMTGMPLNACVGTTANLSINAVNNTTQYVWD
                     APIGSYFDGNPLNVSPYTTSSPNVQVTFGSPNGSFYNVGVQAANACGSTLRKIQKVRG
                     IVSVPATVLGPQTVCENTSNVAYNIAPITGASQYQWSITGDATVTGNGTNVNIDFGPT
                     WNGGTLCVAAQTSCYTSASKCISLSKSAGIPNNLSGNFTACPNTSQTYSVTPLQGAAV
                     YNWTLPTGASGSSNNNSINVTFGNGYNNIGNVCVSVTSICGVTSALKCKTVAPGIPMV
                     PPAITGITNGLCNQSVAYSTPIQTGTSYNWTAPAGANVFGQGNNSVNIQFSTMTTGQV
                     CVVASNTCGTSAPRCVAVKGAPNPPTDIIALPSSWCANTQGIELTANISNTTGSYTLD
                     WGYPSAPVATYVAGGGNSNSLTLDWGTGNGVIAITASNACGSATKTYNATTNCRESEE
                     LTAANFNMYPNPASDVVNIEFWSDKDGMQKDLKVLDLSGRTVMVQKIQTISGRQSVQI
                     SLAQFAKGVYMIELNYQRNKLVIE"
BASE COUNT         7977 a         4887 c         5194 g         8365 t
ORIGIN      
        1 ctatataatt caccattagc agtttttcga ccttcaaaac tcttggcata atatgatgca
       61 ccaccggtaa atactttgtt ttgtgcaacg gacgttaaag tcgttaaact caaagcaaaa
      121 cctatccaat agcttctaag caattttaaa tcaatatgtt tgagtagttt cttgaatgag
      181 taagccggta taagaaataa tattttgttc atctttttaa gtgtatttct tttttaagct
      241 aagtaaagca cacgctcaaa atgcctacat ttgcagtcaa tatttaatat catgtcaacg
      301 gtaaaattca acaataaaga tgcagtattt tttcctgaat taaagcaacg catcaatcaa
      361 tattttgaac aaaataaaat aaagcctacc ggtaatctga aattgtattc taaaaccata
      421 atacttttca ccattttaat agcgctttat attatactgg tattttttac ccctgaaaac
      481 ggatggctgt ccttgttttt agcttcgctt ttaggtttgg taatggcatc tattggattt
      541 aatgtaatgc acgatggtgc acatggcagt tattccagtc ataaatgggt aaatgaatca
      601 atggcacatt cgcttaattt gctgggtggc gtttcattta tctggaaaca aaaacataac
      661 atcaaccatc attcctatac caacgttgaa ggtatggatg atgatataga catcaaacct
      721 tttatacgag tgcatgaagg tcagaagaga tattggtttc atcgctatca gcatatttat
      781 ggtttgcttt tgtatggaat aacttatttc ttctgggttt tttataatga ttttaataaa
      841 tatttttccg gtaaaatagc tgaacatacc cacatgcaga agatgtctat ttgggatcat
      901 gttattttct gggcgactaa aatattttat gttttagtgt ttattgtttt cccaatatat
      961 aatgtaggtt ttattgatac gcttttaggt tatggcgtga tggtagcagt tacaggaatt
     1021 actattgccg ttgtttttca gttggcacat gttgttgaag atatgccatt cgttagtcct
     1081 gaaggtgatg taatgaaaat tgagtcggag tgggctgtgc atcagttaca gacaacaacc
     1141 aactttgcta ctaaaaataa atttatcagt tggttgttag gtggtttgaa ttttcaggtt
     1201 gaacatcatt tgttcccacg cataagccac atacactatc catcaatcag taaaattgtg
     1261 cgcgaaacat gtttggaata taatgtacaa tacaaagaat tccgtaccat gcgtggtgca
     1321 ttggcatcgc atattcttca tctgcgcgaa atgggtagga aataggcaac attgtttttg
     1381 cctgtttagc tcagttatta cttatacgct gtaaattttt cgtaaaaatt tggttaattc
     1441 aaattattat taactttgtg tgaagttttt ctgaaaccac atacatgaaa ttatctttca
     1501 ttcaaggtta ttcacaatat aaaaaggctc tgtttttcat tgatacagac agtagattaa
     1561 accgccagtt tcattctaca acaaataaat taaataagtt aaactcaaat taaaagcagt
     1621 atgaagaaaa caatcacatt gtttatttgc ctgttctcgt tgtcattggc aacttgggca
     1681 caggacaaga cagtaaccgg taaggttact tcatcgcagg acaaactggg tattcccggt
     1741 gtcagcatcc tcgagcccgg aaccaccaat ggtaccgttt cggatattga tggaaaatat
     1801 tccattaaag ttaaatcaac tacaacgcaa cttcggtttt ccggaaccgg tttaacaacg
     1861 cgcacagttg atatcccggc atcaaattca ttagatctta ctatgcagcc tgatgttcaa
     1921 aaaattggtg aagtagtagt aacagcctta ggtattaaac gtgaagagaa gtcattaggc
     1981 tacagtacac aaaaagtaag tggcgatgat ttggcagctg caaaagaagc aaacgttatc
     2041 aactctttgc aaggtcgtgt tgccggtgtt agcattacag gatcaagtaa tatgggtggt
     2101 tcttcaagaa tattattgcg cggtatgcgt tctatcaact ttgaaaacca acctttattt
     2161 gtggttgatg gtattccaat gaataacgaa aacgtggcca cagttgatca gcaacgtgga
     2221 gcattaggtt atgattatgg taatgccatt caggatataa atcctgatga cattgaatct
     2281 ataaacgtac ttaaagggcc atctgcaaca gcactttatg gttcaagagg ttcaaacggt
     2341 gttatcatca ttacaactaa aaaaggtgtt gcccgcgaaa aatcagaaaa gtattctcca
     2401 atcggtgtaa cactaagtac aggatggagc atgaagaaaa tttataatct tcctaaatat
     2461 cagaaccgtt atggtggtgg cgcatcttca gattttattg agagtgatat tcaccccgga
     2521 gagttacgct cagagtttga atatgatgga agctggggtc ctgaattact aggacaggaa
     2581 gtttatcagt gggacagcta cgttccttca atggccaact acaataagaa aacaccatgg
     2641 gtagctcatc caaacaacgt gaaagatttc tttgaaacag gtatcgttaa aaacaacagt
     2701 gtttctttag atggcggaaa cgaaaaatca cttttcagat taggttatac aaactatgat
     2761 gaaaaaggtg ttattccaaa cgagagccta aacagaaata tcatcagctt taatggctcc
     2821 aataaattta atgataggct ttctgctaca ttaaatatga actatgttgt tgcaagaggt
     2881 aaaggtcgtg ctgcaacagg atataacagt attgcctcta acttcaatca gtggtggcaa
     2941 cgtcagctcg atattaaaga gttgaaagat tataaaaacc ctgatggctc acagcgtgca
     3001 tggaatatga atgctgaaga tgatccatct ccactttatt gggataatcc atattgggta
     3061 tcttacgaga attatgaaac cgatcaacgt aaccgtttat atggaggttt gacatttgat
     3121 tatgatatcg gaaaaggttt ttctatagca gtatccggca aaacagacta ctataacgac
     3181 aaccgtcagg agcgtgttgc agtaggaagt gcagttgcaa ctgcaatacc tgcttattca
     3241 gaaaacagta tcacattcaa tgaaaataac tatgacatga tattgtcata caaacatcgt
     3301 ttaggtgatg atttagattt taatggtttt gttggtgtta acagagtaga tcagaaattc
     3361 agcaataaat ttgtttctac tcaaggtggt ttaaatgttc cgaatttcta caacatcaat
     3421 aactcaacca gcagcgaatt aatctattct tctcaggtta gacaacttcg taaaaattcg
     3481 ctttttgcaa gtgcatcttt cggatataag agtttcttat atcttgatgt aacagggcgt
     3541 aatgactgga gttctacatt accagaaggc aagaattctt acttctatcc atcaattaca
     3601 ggaagttttg ttttctccga aaaacttgat ttcaattggt tgagctatgg taaattgcgt
     3661 gcaggatggg cacagacagc tattgacccg gttccttacc gttcaacttt aacttcacca
     3721 agtatgtctg acaactttaa tggtttgcca acaagtgtta tcccaggtac atttaacaat
     3781 accgatttaa gaccggaaat gacttcaggt tttgaaactg gattggaact gcatttctta
     3841 cagaaacgaa tctctctcga tgttgcttat agctcaacac gttcaaaaga tgtaatcttc
     3901 aatgtacaac agtcaggtgc aagtggtatt gattttaaaa tctacaatgc agccgaatta
     3961 accaacaaaa cagttgaatt gatgattaac gttattcctg ttaaattgaa aaacggattg
     4021 gagtggggcg ttggtatgaa ctgggcaaaa accaacaaca aagttgttaa actgtttaca
     4081 gatgagttag gtaatgaaac cgaaaacgtt cagattggaa cagcaccttt ctctgcaaca
     4141 cttcaaatgc gcaaaggcat ggaagcatcg cagattgtag gttatgattt tgtttatgat
     4201 gacaaaggaa acaaagtggt tggtgcagat ggtttttatt tacgttcaga aacagttaag
     4261 cctttaggct cagtattgcc tgattataca ggaggtttta atacctatgt ttcattcaaa
     4321 ggtatccgcc tttcaggttt aattgacttc caaaaaggtg gtaaagtttt ctctcttacc
     4381 aatgtatggg gaaaatattc aggaattttt gctgaaacag cagaaggtga catccgcgaa
     4441 aacggattgg ttaacgaagg tgtaactgaa acaggtgagc aaaacactac agttattgct
     4501 gcggttgacc actattttca ggatggtggt tatgtaatta cagcagctga tgtatatgat
     4561 gcatcttttg taaaattacg cgagatgact ttatcttatg aactacctca atcactgttt
     4621 aagaaagcca ttcgaggtgt atctgttggt gtatatggtc gtaatcttgc tatccttcat
     4681 aaaaacgtac caaacattga ccctgaagca ggtttgtcaa gcggaaacgt acaaggattt
     4741 gaaggtggtc agttaccaac tgcaagaatt tttggtgtta acctgaacgt aagattttaa
     4801 ctttaaaaaa gatagatcat gataaaattt aagataaatc aaaaagcaat tgcaacggtg
     4861 atggtatttt cgctgttgct gggttcatgt aaaaagaatt ttgaatccat aaacgaagat
     4921 ccgaataacc ccaaagaagt accgaatgct tatctgttaa caggtgcaat gcgcggctta
     4981 atggataata caattgacgt atggtggggt ggcaacgtag gaaatcagct tggtcagtat
     5041 tggtcatcta atcaatattc ctcagagagt cgctatctgt accgttctaa tgttaccaac
     5101 tcctattgga gatattttta tggtggcgtt caaaacgatc agttagtagc tgttggcgga
     5161 ttgtatgaat tgagtcagat agttaaaaag tgtaaagaag attcaatcga agcatcgcgt
     5221 gatggctatg ttcccaatca gattgctgta gcaacaatta tccgtgtttg gactttccag
     5281 aacatgactg atatttgggg tgatattcca tattcggaag cgctacaagc cgatggtaat
     5341 aaaacaccaa agtacgataa gcaggaagat atctacaatg gccttctaag cgagattgat
     5401 gatgctatcg gaatgattaa cgatgctgaa tcaggaccaa atggcgatgt tgtttacaat
     5461 ggcgatatga ctgcatggaa aaagtttgct aattccttaa agatgcgttt agctttaagg
     5521 attgtagatc gtaatgctgg aaaagcacag tcagagtttg aagctgctgc tgctggagca
     5581 tttacctcta acagcgacaa tgcattgttg caatatggag atttcccaag cggtaacccg
     5641 atttattaca accgttacat ctccggacgt aatgactatg catcaagcaa caccttcctt
     5701 gattctactt taacaccatt gaacgaccca cgcagagaat gtttctttgt tcctgcaact
     5761 gctaccggac tatggacagg tgaagtttat ggattgtcag aagcaaatgc tgcacaaaca
     5821 ccaaactcac gtgtagcaca acgctctcct ttggttttat ctgctagctt acccggtatt
     5881 tttatggatt atgcacaggt agaatttatg cttgcagaag ctgctgaaag aggatgggct
     5941 gttacaggct ctgcagaaag tcattatgat gcaggtatta ctgcaagcat ttctttctgg
     6001 actgcactta atggtgctgc tgcttctgcc ggtgatattg ctgcatatat agcacaacca
     6061 actgtaaatt atcagactgc cggaggtact tggcaacaac gtattggtgt gcagaaatgg
     6121 atagctttat ttgatcaggg tattcaggga tgggttgagt ggagaagact tgactttaat
     6181 aaactgatga tgcctgccga tggtgttatt gatggtactg gtattccatt aagaatgaaa
     6241 tatcctgttc ttgaacaaac acttaacagt gcaagctaca gtgctgcagt tggtgctcag
     6301 ggcgctgatg aacaagacac acgcatgtgg tgggatgtaa attaatataa tgatttgaac
     6361 tacttataag ggcggaggtt tactccgcct tttttgttta tgctttctta gcataccgtt
     6421 gtttctgata actttgcggc aaatattatc gcataatgag tcacagttat attaaagcat
     6481 tattaaatac tgagccaaca ggtcagattg taaaagcgaa aggatgggtg cgtacaaaaa
     6541 gggggaataa agccatttca tttattgcct taaatgatgg cagcactatt cataatattc
     6601 aggttgttgc agagaataat aactttgatg aatcgttact gaaaaacata actacaggtg
     6661 catgtattgc tgttgaaggt aagcttgttg cctcacaagg acaggggcag aaggtagaaa
     6721 tgcaagccac tcaaattgaa atttatggca ctgccgaccc tgagacattt ccattgcaga
     6781 agaaaggtca taccatggaa tttcttcgtg atattgcaca ccttcgacca cgaacaaaca
     6841 cttttggtgc tgttttcaga ataagacata caatggccta tgccatcaat cattattttc
     6901 ataccaacgg atttttttac atccatacac caatcattac aggcagcgat gctgaaggtg
     6961 caggagaaat gttcagagta acaacattgg acatgaacaa gccaccacgc aatgaagacg
     7021 gtagcattga ctatagtcag gatttttttg caaaagaaac aaacctgaca gttagcggac
     7081 aacttgaagg tgaactcgct gcaatggcat tgggaaaaat ttacactttc gggcctacct
     7141 ttcgtgccga gaacagtaat acgccacgcc acttagcaga attctggatg atagagcctg
     7201 aaatggcttt cttcgacctg aatgataata tggatctggc agaagatatg ctcaaatatt
     7261 tagttcgtta tgcactcgat aactgtatgg atgatttgga gtttctcaat aatatgtacg
     7321 ataaggaact gattgggcgt ttacgcagtg tcatcgaaaa tccttttaaa cgtttaacct
     7381 ataccgaagc aataaatgtt ttggaaccaa acaacagtaa gttcgaatat aaagttagtt
     7441 ggggtgccga cctgcagaaa gaacatgaaa actttcttgt agaacatttt aaaacacctg
     7501 ttattttaac tgattatcct gcttccatca aagcatttta tatgaagcag aacgatgatg
     7561 gaaaaacggt cagagcaatg gatattttat ttccatggat aggtgaaatt attggtggct
     7621 cacagcgcga agaaaattac gataagttag tcagccgtat gaaagccatg cacattcccg
     7681 aaaaagaaat gtggtggtat ctcgacacca gaaaatttgg tgcatgtcca catgctggtt
     7741 ttggtttagg ctttgaacgt ttgatgcttt ttgtaaccgg tatgaataac atccgcgatg
     7801 tcattccatt tccacgcaca ccacgcaatg cagagtttta attttttctg attaaggtaa
     7861 atttaattct ttaagttttt ttgcttttat aaaggtaatt aataccacaa gcaaggtcat
     7921 tcctccgcca aagagtacag aaggtattaa gcccatgagc tttgccataa caccagattc
     7981 aaatgccccc aattcatttg atgagcctac aaaaatactg ttcactgccg atactcttcc
     8041 tttcatttcg tcaggtgtaa aactttgaat aatggttgag cgaatcacca cacttacatt
     8101 atcaaaaaca ccgctcaaaa acaacagaaa aagagagagt aaaaaactgc ccgaaagtgc
     8161 aaaccctatc atgcataatc caaatccaac aacgcttgcc agcattttat agcctgcatc
     8221 ttttcggggt ggatgaaatg ctaagaatat ggccattact actgagccaa cagcaggtgc
     8281 agcacgcaac aaaccaagtc cttgtgcgcc tacatgcaaa acctgatcgg caaaaacagg
     8341 aagcagcgcc acagcgccac caaaaagtac tgcaaacaaa tcgagagaga tggctgaaat
     8401 gataaattga tttttaaaaa caaagttgac tcctgttttt aaagattgta aaattgattc
     8461 tgttttgttg tgatagtcaa catgcctgtc tttaatcagt aaataacaaa tcattgtaac
     8521 aatgataaaa gcacagacta ccaacgaaga ataaaataca ccaatccaac catagaaaaa
     8581 accacccatt gcaggaccaa taactgctgc cacctgccaa aggctgctgt tccatgttga
     8641 tgcatttaca taatgttctt tgggcactaa ctgaccccaa aatgctgcct gcgtaggtgc
     8701 atgaaatccg cgtgccaatc caatgagaaa aatagtaagg taaattggcc aggtagaaat
     8761 tttgccaaaa tcataaacat aaattgccag gaatgctgaa gcagaaaggg tgatggcaca
     8821 actccatatt agaagcttgc gtctgctaag tctgtcggcc aaatgcccac caaacaaagc
     8881 aacactcaat gcaggaattg cttcaatcaa accaattatt cctaaagaaa gcggatcttt
     8941 ggtcatctca taaatcagcc agctgagaat aatactctgc atttgaattg ctattgtcag
     9001 acaaacttta gctatcagaa acaaacggta atctttatgc ttccaggcta caaatgcatt
     9061 tcttgttttt gtcataaaaa aattaaggcc aagcatttat gctcagcctt attttcagat
     9121 atcatttata agattaatac tcatcttcgt tgaaaaaaaa gtcatcctta gttggataat
     9181 caggccaaat ttcttcaata ctttcatacg attcaccttc gtcttcaagc tcctgaagat
     9241 tttcaaccac ttctacgggt gctcctgaac ggatggcata atcaatcaat tcgtctttag
     9301 tagcaggcca gggggcgtct tcaagatgcg atgctaattc cagtgtccaa tacattacaa
     9361 tattcttatt aaaaaattaa actaattgtg ccttaatttt taagggctgc aaaaatatgt
     9421 aaattaaatt ggaaaaaaca agtgtttttt ttaatagtta ttaagattct aaactcttgg
     9481 tctccaggga gtttctgtta tatttcggac aaagcataca tggcgtgcca acataaaaag
     9541 atagtcagag agcctattta ggtagattag taccattggt tctacccatt ccgactcgct
     9601 cagaccagta gccagacgct ctgccctgcg gcaaatacaa cgtgccacat gagtgtggct
     9661 gtttaacaga tgaccaccgg gcaaaacaaa tgacttcagt ggtggcagtg cggctaccat
     9721 tctgtcaatt tccttttcta acagcgtaat gtcttctgta tttaaatcag gaattttcat
     9781 tttcgacttt tccggatcag cagctaatga cgagccaatg gtaaacagtc tgtcctgtat
     9841 ttcagccaat atttccttac acaggtcttt ctcttcggca agatccttca gtagccctat
     9901 ggtagaattt agctcatcaa cagtgccata ggcatcaata cgcagatggt gtttgctgac
     9961 tcttgtaccg ccaatgagtg atgttttgcc gctgtcaccg gttttggtat atattttcat
    10021 tgattaatca gataaataat aatttttgta caacaaacct attaacattt tgttactgaa
    10081 aagaagtgac agaagttaaa atgttaacag ctttggctgt tttctttgat agttaaatta
    10141 aagtttgtta aactatctta cagcatgttg cttaccatag atcgcatgga ttaattttgg
    10201 tatgtttact ttctgtaaaa acggatattt aaagttcaat tataatgttt aaaaaagtag
    10261 gattagtatt attactgtta gcgtattgcc gaagtacatc agcacaaaac atagaaattc
    10321 aaaagttggg tgcattgctt cagatgattg attattatta tgtggacact gttaacaaac
    10381 caaaattggt tgacgatggc atcagagcta ttttaaaaga ttgcgacccg cactcacagt
    10441 atattccggc aagtgagttg aaagaaatga acgagccatt ggtaggtaag tttgaaggtg
    10501 taggcattca attcaatatt cttgatgata ctattatggt aacacaaact atagccggtg
    10561 gtccatcaga aaaattaggt atcagacccg gtgaccgtat tgtaaatatt gacggaaaaa
    10621 aagtggctgg agttaaaatt accaataagg atgtcatcag caaactcaga ggcgacaaag
    10681 gcactaaagt aaaagtggat atgtacagac gtggcgaggc agaactttta acttttgata
    10741 ttacacgcga taaaattcca ttgtttagtg ttgatgcatc ctataaaata gaacccgata
    10801 tcggttacat taaaataagc cgctttgcag ataataccgt agaggaattt aaggaagcac
    10861 ttgccaagtt aaaatcgcaa ggagtaaaaa gtctgattct tgatttaaca agcaacggtg
    10921 gcggatatct gaatcgtgca catcagttgg ccgatgaatt tttaagtgat gggaaacgca
    10981 ttgtttatac agaaggccgc tcacagtcca gagaagatta tttttctact gcagcaggtg
    11041 gatgggaaaa aggcaaactt gttgtcatga taaatgaata ctctgcatcg gcaagcgaaa
    11101 ttgtttcggg tgccatacaa gattgggaca gaggtttgat tgttggccgc aggtcgttcg
    11161 gaaaagggtt ggttcaaaaa ccatattcat tgcccgatgg tagtgccgta agacttaccg
    11221 ttgcaagata ttatacaccg tcaggacgtt gcatacaaaa gtcatatcag gaaggcgatg
    11281 aagaatacag tgacgacatc agcaaccgtt ttaagcatgg cgaactctat catgccgaca
    11341 gtattaaatt caatgactca cttaaataca ctaccaatgc caagcgtgtg gtttatggtg
    11401 gaggaggtat tatgcccgat gtgtttgtac cattagatac catgttcagc tctaaatatt
    11461 attcagattt aatccgtaaa gcagcattaa gtgagtttgc attaacatac gctgacaata
    11521 accgcagtaa attaaaatcg gcatacacaa cggtagaaaa ttttaaaaag aattttaatg
    11581 ttgacgataa aatgatgaat gactttattg cttttgcaga aaagaaagaa gtgaaaaaag
    11641 acgaagaagg attaaagcgt tcaggcacta tgattaaagc acagataaaa gcactcattg
    11701 cccgtgatat ttttaatttc ggagcctact tctacatcat aaacgatagt gacgatacat
    11761 ttaataaagc agtagaatcc attaaggata atacttttag taaaatgaaa attgcaaata
    11821 attagattta ttattccttc gtgaatctca gcatttgtat ttgatgtaat gcatttttta
    11881 tgctgatgaa ataaatccct tttgtaaaag ttgttttaat tttttctttt gtattacttg
    11941 ttataacaga aagtacaact ttacctactg catcaaacac agttaaaaca ttttcgccat
    12001 ttgtaagtcc agaaattatt aattcgtctt taacagggtt cggataaata cagatgttag
    12061 cacttccaat gttatttatt gaggttatca atatatatat tgaatcgcta atcttggtgc
    12121 aaccattgac atcagttacc attacactat aaaatcctgt agcagtgggt atgtaacttg
    12181 tagcggtggc gccaccaatt ggtagtcccg ataacaacca ttgattgccg ctgacagacg
    12241 aactttgaag agtgttggca atgttggtta taaaaggttg tggtggaatt ggattaacaa
    12301 taatgttgtt tatgatactt tggctacata ccaaattgga tgcaacaaag gttaacgaat
    12361 aatttccggc acttaaacca gtgagataaa atgatgaatc atttgagtat gatgtaccat
    12421 ctaaaagcca ttcactgttc atatcaaaca ataatgcaga gttggcttga atagagtcgt
    12481 tttcgcaaat ggtggagttg ctaagtgtta ctgtggccat ttggttgcaa tcgaaaacag
    12541 tattggtaac agtgttggta attacgggcg aattaaaatc aaaataaata gctgcattat
    12601 tttgtataac tgtaccagca gcatttccgg gtatttgatc tattttaaat ttaatataac
    12661 cgtggcttgc cggttcgttt gaattgctgt ctggtaataa aatattatcg aaatgaaatt
    12721 ttaccacatt gcctgaagca aattcatagg tgaaagggtg agaagtgttc aatacactta
    12781 acgaatattt gttgagtgat aatggaagcg tgtccgcaat cattactgca aaagcagtat
    12841 cagttccggt gttttggaaa cggatggtgt atttgagttg ttgtgcggct gtaatgtaac
    12901 ctgcagcatc aaaaccatgc ggcattacag ctttatcatt agggtcaaac gaaccggcta
    12961 ctgtttgtga aatggaatca atattatttg aaatgatggt ttcaccaaca actgttgtta
    13021 tcaaagcaga agcatttaat actgttccta atggtgtagt agatggaaca acagtattaa
    13081 tctgaaatga tgcagtgccg aaaggaggca gtgatatacc tgtaaacatt aaaactccgt
    13141 tgctgttggt tgccggtgca ggaacagcat ttacaaaatt taaagtggtg tcataactca
    13201 ccgttagtgt ttgagccgga attatttttg gagtaagatt gcgaacatca attttgtaat
    13261 gatgattaaa tccgggtcga ggttgattgc cactaatgcc aacttgtaaa tcgggattta
    13321 cgtttacact atcggccact tgtacatcaa ctaatgttcc gggtgtagta atgttaatag
    13381 taaatgtggt agtatcagga catacctgcg caaattggta tggtgcatat tgtttaagtg
    13441 tgtaggtgcc atttactacc ggaataaaaa attcaccggc agcattggtt acagaactat
    13501 aaccgccagg attaagttct accaatgttc ctgaaaataa aatatcaccg aaaccataag
    13561 aacagtttga gttataatca ccaaacacac gaccttttac caaagaacaa ttaggatcat
    13621 taacaattgt aaaaggtgtg gaataaatca aaatagaatc ctgtatgttg ggtatgcctt
    13681 gtgagtagat agtgtcgcct gtaaaaactt ttaaagtgta atttccggga agaagattct
    13741 tgtaatcgag taatgaatcc tgtacagcac gataccctac tgtgttgtta acagaatctg
    13801 ttattatggt tttgtatttg gaagtaactg catttccagc tcctgtgtag cttgaaatta
    13861 aattgctgtt aaaagtattg ttgttaattg cagggtaggg gtgaagatta atagaaagat
    13921 ttcctgtagg agaaccattg catgcatccg atacagaaaa agttggatta atagtgggca
    13981 gttctttaat tcggaattcc acctgggtgt agcatccgaa tggtgccacg ccatgcacat
    14041 aatatttgcc agcaggtaag ctgtcaaaaa tgtaataagg atctatggct ccgcctacat
    14101 aataactaaa tgtatgtgtc gcgagcaaat ttaaagcagt gctgtcatac aaattgtaat
    14161 tataagtacc ttgaggtatg tcattagccg aaggacctgg ataaataact acttgaaatg
    14221 caccgttatt tgagcccggg tttgacatag caattgagat aacagaaaaa ggcacttcaa
    14281 cttgggcatt caccatgccg ctgctttgtg ctgagtcgcc ttgtgcatca ataaccaata
    14341 aatcataaat gccggcagga acacccaata aagtatcagt gccgggaatg ccattccaaa
    14401 agaaggagta tggtgcaaca ccaccgttta cctgagctat taacgcccct gagttactgc
    14461 cgcaccaggc acttgtccat tgtataacaa acgcagaaat gggcatttgg ttattctcag
    14521 cttttgcctg ctgaataata gtaagaaaaa agaaacttaa tattaatttt ttcattgaat
    14581 aaataattag ttgaactata aggaattttt tcaattacaa aattatatag tcataatcga
    14641 ataaagaata gtaggaaaaa aaattaaaaa aaattggctt gaatatagat tgtattaatt
    14701 gaattaggag ttaagatgaa attggcaatt tataattata gataaatccg gttttccaat
    14761 taattcaaat gagttaattt atttgaagct attttattag gaaaaataaa cggttataga
    14821 aaatggcatt tcataagtct ataaacttgc aaaacaaaaa tagaatgata aaatagcaat
    14881 attagcaatg ttattatgct acatgcccgg ttttaaaaaa caactcaaca gacgagccat
    14941 tgtttgaaaa aataatctga tcggctaaat gtttcattaa aaagacaccg cgtcctgttg
    15001 gcttttcaag attttcggga gcagtagggt cgggcaggtt ataatagtca aatccttttc
    15061 cctgatcggt aatagcaaaa gaaatagtgt cgctttctac ttcgtagctg attttaactt
    15121 tcaggtttgg gttgtatttg tttccatgct gtatggcatt gtttacagcc tctgttacgg
    15181 ctaccagaat gttgccataa gcatcttcgt gaatgttgtg ttcgccttta agatcgtcaa
    15241 ccaaacgctc tataatatgt atgctttccg gtttagattc tatctgaatt gtctggatgt
    15301 tttttgttgt catctgtctt aaaattgaag tccttgaaaa taattattta ccaaattttt
    15361 gtaaaatgta tttaaacccg gaggtactgt attcagtagt tccgtttgtt tttcttttag
    15421 ttttttatac tcctcaaaag cggcagggtt acgtaaaaat tgctcttttg cttgctctgc
    15481 cttacgtgtc tgatcttgtt ctctttcacg ctgcgcattc tccgcttcaa gcagtcgtgt
    15541 aagtatttcc tgctgccttt ttaaggtttc ttgcgttatt tttttattca caatatcgcg
    15601 ctcgttttgc tccatctgat tggccaaatt accgaggttg cccatagagt ttttaccatc
    15661 tttattttcc tgctggttca actggtttaa ctcatttctg atagcctcct gttgggcagc
    15721 cattctcgct aactcttcgc tcataccttt gtcaccctta cccccgtttt tttgcattcc
    15781 ttctaacttt cctttcattt ttttcatttg ttcagaaagt tgttgttgca tcatttttaa
    15841 tttttctgca gatggtcccg gtttgccttt gcccggtttc ttacatgagc cattgccctg
    15901 cttctgttgt tgctgctgca tttgcatttg ttgtaatgct tcactgagca gtagtgccag
    15961 attattggcc gatgtcataa tgtattgctg cgagctgcga gccacaggaa cctgcctgtc
    16021 ctgcaaaaat gcaatagatt tttgaatatt tgactgaatg ttagaaattt cagtatttac
    16081 cgtctgctga atctgaatta cacgcttgct aagggcaaat aatgagtctt caataatttt
    16141 caggtcgtcc tgcagttttt tctgcctctg acccattttt acatactgtg gattgttgac
    16201 atctgtagtc tttaatgcct gcatcagtgc ttcctgctca aatgaaaaac gaagcaggtt
    16261 ctctaataaa taccttaatg atgccatgtc ctcttccgat tgttccgatt cactctgtgc
    16321 catttggttt ttcattttct gcgacatgtt ctgcatcttt tccgcagcat ttttttgcga
    16381 tttaccggca ttcgactttt tgttttcgtt caactgctgt tcgctgttct gttgctcctg
    16441 atctatgtct ttttgctcct gctctgtgtt tgccagctcc tgtggaaatt ccagttcctg
    16501 atttttcttt tctaatgctg caatatcttt tttaatgtct tcaaactgtt tgttcagatc
    16561 cttctgttgt tcttttaatt tttctgtatc ggaattttta tccatgcttt gctccgatag
    16621 ttttttctga tcttctgcca gtttatctaa attttctaat gtgtttttta acttttgttc
    16681 tacttctaac tgcttaaaca attccaaagt gcgatccatt tgtttttcaa gatccttatt
    16741 gttgagcttc attttatcta acgcttcttg cacactttgt ttgtcgagtt gcgaaagcat
    16801 ttgctctaat tgcttcatca gctcttttaa ttcaggattc tgcaaacggt caaacaattc
    16861 ctgtatctgt tgctgttttt cggctaactg actttcattg ttcttgaact cattctgctt
    16921 ggcaagattt tgttgttgct cattttttat cttgtctaac ttttcgtcca actctttttg
    16981 ttgcttcaac aaatcttcaa tacgcttgcg gtcttcaaag tcagtttttt tcttttctgt
    17041 cagcttgcgt gcagcatcgg taatttgttt ttgaattttt gatgcctgtt taagactttc
    17101 ctgcaagtct tttttaattt catcgttact tttctctgta ttctctgcca attcacgaag
    17161 cgtaggagct ttaaaaatta acgattgcga tttagctgat tttggcccat tgacaccgtc
    17221 attgtcccaa acttcaaaat aatattcaac ttcatcaccg ggtactacac catgcaatgc
    17281 accatcccaa aaatgataaa aatctgtctg attgttattc ttgttgattg taagaatttc
    17341 tgtatttagt ttatcgctat gtgccgatga atcgttttta ctttttacaa aacgataatg
    17401 aaaagccaat ttcgaaaaac cataatcatc ctttacagtc cctttaaaaa atattctttg
    17461 taatgatgat gaatcatttt gttgttctac ctgaatgata ggatactgat cgggaattac
    17521 gttgatggaa tattctaaag tgtctttatt ggtgataaaa ttattggcag tggcaatgga
    17581 ataattagca cctgaaagca ttcttttgct gtaggtgtat tgattgttgt ccgacaccaa
    17641 tggagtgagg ctgtcattaa accaaagttt gaatactgag gtattaacgg tgttgaatgt
    17701 ccatttcaca atagtgcctg caggtattaa taagtcgcct gtattattca gtgtttcgtt
    17761 tttcttttga agataggcag gatagtttaa atcaattttg aatgcagaaa ttaatggatt
    17821 gggtactgat ttcaattcat aattttcgga ataaaaacca tcagccatca attgaaaaga
    17881 tgtgcttttt atgatgcgat gaaaagtaaa agtaaaatgt tgtttatctt ctttgtcggc
    17941 tttaatctgt cttccttcat aagtcacata aacttgatcg ggtagttcct gcccttctac
    18001 tttaaagtta agtgtaaagt cattctgttc aattacatta aagtttttat tgagtagaat
    18061 aaaattaaaa ggtgcaggtt ttttatacgc tacattgtaa ttgacaagtc tgcttgtact
    18121 ttctttaata acgtttggtg ctgcaaacag cagaataatt aaagtgcctg caggtattaa
    18181 agctaatggc aaatattttc tgttcttttt aaaatcaata gcaccggtaa atggaatagg
    18241 tttcaggttt tgaattttct gatcaatgcc ggcctgaacc aattcattgt ggttgttttc
    18301 gctgagttca ttaagttgaa gcgtattgag tagtttatct tttacatcag taaagtggtt
    18361 gccgataatt gttgcagctt gctcgtaaga aagagttttg cctaatttcc agattttaaa
    18421 aagaggaata actattaatc tgatgatgac tgcaagaact gtaaatacat aaatatagaa
    18481 aagtgctgtt cggagtgaag agcccatcca tgcaaaatat tctgccaaag aaatgaaaag
    18541 aaaaaatcca atagttattc ctgtagcata aatgctccct ctgattacct gattgcggta
    18601 atacttacga ataaactcat cgagtttttc aatcaataga cggtaatttc ctgtcatgtc
    18661 cctttgttca atacataacg ctaaagtgtg gtttcaggtt atgtctgacc ttaaatttta
    18721 ataaacattt tacagcctaa aaatagtaaa aatgagcagg ccaaatgcca tgcatttgcg
    18781 aatattgcat gaagaggcaa acaaacaaat gttaaaagaa agtaagattt aaagcagttt
    18841 aaacgattcc agaaatttag ttgtgtaatt gccttttcgg aagtcttcgt ttttcatcaa
    18901 ctgaatgtga aaagggatgg tagtttttac accttcaatc acatattcac ttaatgcacg
    18961 ctccatagta tttattgctt cttctctggt ttgtgcaata gtgatgagtt tggcaatcat
    19021 actgtcgtag tttgaaggaa tagtatagcc tgcataaaca tgcgagtcaa cacgcacacc
    19081 gtgtccgccc ggaacgtgca gattggtaat tctgcccggt gatggtctga agttgttgta
    19141 tggatcttct gcattgatac gacattcaat ggcatgcatg gtagggtagt agtttttacc
    19201 tgatataggt tcgcctgatg caatttttat ttgctcttta ataaggtcgt agttgattac
    19261 ttcttccgtt acagggtgtt caacctgaat acgggtattc atttccatga agtagaaatt
    19321 gcggtgttta tccacaagaa attcaactgt accaacacct tcgtatttta cagctaaggc
    19381 agctttaatt gctgcttcac ccattttttc gcgcaattct tttgtcatga aaggcgaggg
    19441 tgtttcttca acaagtttct gatgtcgtct ttgtaccgag caatcacgtt cggagaggtg
    19501 acatgctttt ccatattgat cacctgcaat ctgtatttct atatgtcgtg gttcttcaat
    19561 atatttttcg agatacatac catcgtttcc aaaggcagcg cctgcttcct gacgtgcact
    19621 gtcccatgcc gctgcaaact cttcgtcttt ccacactatg cgcattcctc ttccaccacc
    19681 accggcagtt gctttcagga tgacaggata ctttatttca gcagcaattt ttaaaccttc
    19741 ttcaacactg gtaagtaatc cttcagagcc gggaatagtt ggtacaccgg cctttttcat
    19801 agtgtctttt gcattgcttt tatcgcccat gccatcaatc tgttcaggcg tggctccaat
    19861 gaattttatt ttattcttgg cacaaatagc gctgaacttt gaattctctg ataaaaatcc
    19921 atatccggga tggatagcat cagaattggt gatttctgct gctgcaataa tattggggat
    19981 attaagataa gaatctttgc tgggtggagg acctatacaa acggcttcat cagcaaagcg
    20041 aacatgcaga ctttcgcgat cggcagtaga atagactgcc accgttctaa tatccatttc
    20101 tttacaggtg cggatgatgc gtaaagcaat ttcacctctg tttgcaatta atattttttt
    20161 aaacataatt agtttggtaa tgatgaaatt aaaaaagcgc caaggagctt ataattctaa
    20221 gaaaaatatt gttgaatgaa tcaggatgga tcaattaaaa acaacggttg gtcgtattct
    20281 acaggagtgg aatgttctac caaaactttt acaattttac ctttaatgtc actttcaatt
    20341 tcattaaaca gcttcattgc ttcaataata cagattacct gaccttcttt tacttcgtca
    20401 ccaacattta caaaatttgg tttgtcgggc gatggagttc tgtaaaaggt gccaatcatt
    20461 ggcgacttga tggttttgta ttttgaatca tcacctgcag tttttgcggc aggagcggct
    20521 gcaactgcag gagcagcaat tacttgttgg ggttgagcag gtgccggcat ttgcatgact
    20581 ggctgaactg ctggtgctac atgtacaatt tgctctttgg ttgggttttt aatggttatt
    20641 ttaaaatcct tttgttcaag gtgcacttca ctcactccgg ctttggctac aaagcgaatt
    20701 aattcttcaa tttctttttt gttcatatac tgttgttttt tacaagtctt ttcgtgaaaa
    20761 tgaatcaatt ataacataac cgctacaata ggtcatttca aaagacagca tcaaaagtag
    20821 aaacttttta caaatatgat gaaatgggca aaaaagaaaa aagggcagcc ttatgggctg
    20881 ccctcgctta ttttggtttc agcgctgccg gtttactcta taaccagctt atttctttga
    20941 taatttagct caatcatata cactccttta gcaaattgag caaggctaat ctgaactgac
    21001 tgacgaccgg aaatggtttg aattttttgc accataacag ttcgtccgct caaatcaaga
    21061 actttaagat ctttttgcat gccatcttta tcagaccaga attcaatgtt tactacatct
    21121 gatgcaggat ttggatacat attgaagtta gctgcagtca actcttccga ttcgcgacaa
    21181 ttggttgttg cattataagt tttagttgca gaaccacatg cattacttgc tgttattgca
    21241 attacaccat tgcctgtacc ccagtcaagc gtaagactgt ttgaattacc cccaccggca
    21301 acataagttg caacaggtgc agaaggatag ccccagtcaa gtgtataaga accggttgta
    21361 ttgctgatat tagctgtcaa ttcaatacct tgtgtatttg cgcaccagct tgaaggtaaa
    21421 gctattatgt cagttggagg atttggagct ccttttacag ctacacaacg tggagcactt
    21481 gtgccgcatg tgttacttgc aacaacacaa acctgtcccg ttgtcatagt agaaaactga
    21541 atgttaacag agttgtttcc ttgtccgaat acattagcgc ctgcaggtgc tgtccagttg
    21601 tagcttgtac ctgtttgtat aggtgtactg taagctacag attgattaca cagtccattt
    21661 gtaatacctg taattgcagg aggcaccatt ggtattccgg gagccacagt tttgcatttt
    21721 aatgcagatg taacgccaca aatacttgta acacttacac aaacatttcc aatattattg
    21781 taaccatttc caaaggtaac atttattgag ttgttgtttg atgaccctga agcaccagtt
    21841 ggtaaagtcc agttataaac tgctgcgcct tgtaaaggtg ttacagagta agtctgactt
    21901 gtatttggac atgcagtgaa attacctgaa agattatttg gtattccggc agatttgcta
    21961 agactaatac attttgatgc actggtataa catgatgttt gtgccgctac acaaagtgtt
    22021 ccgccattcc aagtcggacc aaagtcaata tttacattgg ttccattgcc tgtaaccgtt
    22081 gcatcaccgg taatactcca ctgatattgg cttgctccag taattggtgc aatgttgtaa
    22141 gcaacattac ttgtgttttc acaaacagtt tgtgggccaa gcactgtggc aggcacagaa
    22201 acaatacctc ttactttttg aatttttctt aaggtacttc cacatgcatt tgctgcctga
    22261 acacctacat tataaaatga accattcggt gatccgaaag tcacttgtac gttaggtgaa
    22321 cttgttgtat aaggtgatac atttaaaggg ttgccatcaa aataactacc tataggtgca
    22381 tcccaaacat attgtgtagt attattaaca gcattaatcg aaaggtttgc agtagttcca
    22441 acacaagcat ttaatggcat ccctgtcatt ggcacagtaa ctgctgcaga aggaacacca
    22501 actgtaatag ctaatgataa atcagcacat ggtgtggcac cacaaacatc ttctgcacgt
    22561 acataataag atgtacttga tgatggattt actgtaattg atggtcctga accaacaggc
    22621 gttgtaccac aatttgtttt ataccatttc caatcagcag tagatcctaa agaaccacct
    22681 acagcagata gtgttgttga accacctata caaagttctg ttccgttttg tgatgaaaca
    22741 gacaatgcag cagttgatgg tgtatttaca agaattaaat cttgttgctg agtgcttgtt
    22801 gcacacatgt gagcatcttg taccattaca ttataacttc cggcagttaa gttgagattt
    22861 gcattgttgt ttacataagt actgccgttg tcataactat acatgtatgg tgttgtacca
    22921 cctgttgcat tcattgttat gctaccaaat gttgatggac attgctgatc tgtatatgtt
    22981 ttggtaaact gcagcaatgc cggctccgtt attgtagctg taactgtatt ctggcaagca
    23041 ttggcatcac taacatagat tgtataggaa ccggcaggaa gtaaatcaaa ctcacctgtt
    23101 gtattaaagt ttacgaagtc gtaagtatag gaatatggtc ctgtaccatt tgaagtgttg
    23161 gtagttattg atccattgct ctcaccaaaa caatttacgt taatctgtgt ggaaacattt
    23221 attacagggt tcggatttac atcaacctga acagttgttg ttgcagtaca tagatattga
    23281 tttgttacaa caacagtata tgttccgtta tatgcagaag tagcactaaa aatattaggg
    23341 ttctgctgag aactgaaagg aagattgttt ggatcatacc actgatagct atttccgctt
    23401 ccttgtcccc cggctaagtt atctgcaata aagttaatat catttccatc acagacaggc
    23461 gcattactgc ttgctattgc caaaggtccc ggtgttgcac ttacaccaac agttgtgttt
    23521 gcggtgcaac cattagctgt tgcagcaaga ttatatgttg ttgttcctgt tacagtagca
    23581 gaaggatttt catctgttga attgaatccg ttaggtgttg atgtccaagt ataagtaata
    23641 cctgaagagg tcataatggt aactgtccat gaaacaattg ttcctacatc gccactggct
    23701 ctgtcagaca tgttaagtac ccagtttcct ccggcattca ttgggaatga gattccgctc
    23761 caatttgtat ttattgaacc atttgtattt gaaggtgcat aagttccggc aggaatcgtt
    23821 gtagggcccg agtttgattc tggaagaact gaacctgcac ttacatcaaa ctcataatag
    23881 ccaccaaaat tctgaccatt tccaaaactt gaagcaggta caccaagacg gtcaaacaca
    23941 gttattactc ctcctggtga agttactttt gcaataacat cacctgaata ggtatgagta
    24001 agattaatac caattttcag ctgggtagca gaagcaatag tttgtgcagg aattgttatc
    24061 gttgaattta ctccgcttac attgttatcc ggtatgctta cgttaggtga agcagaataa
    24121 gaaaccggta atccctgatt tgcatttaat gttaccgcag aaccttcaca tactgttgca
    24181 tttgaaacag taggagtacc tgttggagcc tggtttacta caactgttgt tgttgctgtt
    24241 gaagaacatc cacctaaggt aacagtaaca gtataagttc cattcattgc taaggttgca
    24301 ttgctgcgtg ttgggttttg cgtagaactt gtatatcctg cagggccact ccatgcataa
    24361 gttgcacctg atggacttgc attaagattt aatgttgttg cagtacatac cggtgcattg
    24421 ctcgatgcag atgcaatagg agcattatta acagcaacag ttacacttga tgtattggta
    24481 cagccggcag aactggtaac aacaacagta tagctaccat catttacaat ctgagcattt
    24541 gaaattgaag ggttacgaat tgagcttgtg aaaccatcag ggccggtcca tgcataggat
    24601 gcacccgata cggatggtgt tgaaagatta attgtgaaac caacacaaac aggtgagttg
    24661 ctgcttgcag ttgcagtagg taatgcatta accacaacat ttgttgttgc agttgcagaa
    24721 catcctgcaa gaggatcagt tgctgttaca gtataaatgc ctgcatttgc agccgcagca
    24781 ccgcttattg atgggtcttg caatgagcta ttaaatccgg aaggtccaga ccattggaat
    24841 gttactccag aagcaactgc atgtagttgt attgtttgac ctgcacatac agcaccatca
    24901 ttacttgcag aaacagatgg tgcagcatta actgtgacag aaacactatt atttgtgctt
    24961 attacagaag aacaaattcc acccggagca cctgaactta gattggtaac agtgattaca
    25021 ggtgaaccac ttgtggttaa tcctgtcgct gtaaaagttc cactaccggc agtagtaact
    25081 gtcatagtag ttgttaatcc ggctgcagca ggtaaactgc ggttataagt tactgtataa
    25141 gatccaacag gtaaatctgt aacagagcct gttaaactaa taacagaagt gcccggtaaa
    25201 ggacatatag gagtaacagc agaaattgct gtaatattat aagcaggaca aacccaagta
    25261 acaattacct gacctctgta tccatcacca ccagtgcggc ttccccattg gctgctgctg
    25321 tttctaccac ctgcaccacc acctgacaat gctgaggcat cagcgccatc accattactt
    25381 gatcttccat tagcaccatt tccaccacca ccggatcctc cggttccacc agtcattcct
    25441 gatgcattac caccgtttcc tgaagtacct gcaccaccac caccaccacc ggaattgcca
    25501 cccgatgctg ctgcaccatt accaccattt ctggttccaa aggcaccatt acctcctgca
    25561 ccatttgtag gagatgtgct tccatgtttt cctccaagac caccactaac agttactaag
    25621 ccaccaaatg aagaggagcc accattattt ccatcggcac cgcctactcc ggcaccacct
    25681 gctccaacag taacagttat tgtactaccg ggaattacag ttacagatga gtttttcgcg
    25741 taagcgccac caccaccacc accaccggca cgtgtaggag gaaatattcc tgaggccaca
    25801 ccaccaccgg caccaccacc tccccaggct tcaacctgaa cagtagttac tccggcagga
    25861 actgtaaatg ttcctgatga tgtgaatgtt tgtgtttgtg caattgctcc tttaaaaaag
    25921 aacaaagcga taaaaacgaa taccactttt aatgcactga ataatttttg cgggataatt
    25981 tgtttttcca tttttagtga tttttgttat taattaaaat tttacccaag caatttagga
    26041 taacatcgtt gctcattgaa ttgtaatttg gtgaatggca aattttttag ggcgaaaatc
    26101 aaaaaacata aagcgacata ttccgtccgt gcttttattt acgcgattat gctgtaaata
    26161 gccgcacatg tggtctttaa agccatgttt ttgattgtta ctaaatgcga atggaattac
    26221 tatttttggc ataaattaaa atacagatga aacatttaat ttggcttgtt ctgatatata
    26281 taatgagtgc ttttagtgtt aaagcacaag atccaacaac cttaattcaa cgaactccaa
    26341 taaaatcatg gtatgccatg aattccggag aatttatttt ttctgcaggt gaattgaaga
    26401 tatctgataa ggcagtttat gat
//
LOCUS       DAORQT010000004        22411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_144_length_22411_cov_11.120997, whole genome shotgun sequence.
ACCESSION   DAORQT010000004 DAORQT010000000
VERSION     DAORQT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144_length_22411_cov_11.120997"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK134_00360"
     CDS             <1..467
                     /locus_tag="PK134_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRB37336.1"
                     /translation="YMLQGTIQEPGGTSQALWGYSVFKNGNDVAGKTGTTSNYSDAWY
                     MGVTHDLVTGVWVGAPYRSVHFRNSSGQGSRLALPIFGKMLEKAYQDPKSGVVVGKFK
                     KPKKISKTIYCENDDIELEAADSLLNDSLINTELMILESDSLKSDSLQGGGD"
     gene            495..1250
                     /gene="tpiA"
                     /locus_tag="PK134_00365"
     CDS             495..1250
                     /gene="tpiA"
                     /locus_tag="PK134_00365"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRB37337.1"
                     /translation="MRRKIVAGNWKMNGTLDDAIKLTSEIVEMYKDEINSDVQVVLSP
                     PFPFLNNVSKLTNSTAIKIAAQNCAAQATGAFTGEVSASMIASCGVGYVILGHSERRT
                     YFNDTDEVLLLKVKQALLNGLQVIFCIGETKTERESNNHFECVENQIKNVLSNLTSDE
                     FAKCIVAYEPVWAIGTGLTASSAQAQEMHYFIREVLKRQFGEQASETSILYGGSCNEN
                     NAKELFALPDVDGGLIGGASLKSRSFISIIKSF"
     gene            1255..2421
                     /locus_tag="PK134_00370"
     CDS             1255..2421
                     /locus_tag="PK134_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRB37338.1"
                     /translation="MINSFNCVLLFAFLFCGLFSDAQSLRQPAFVEYSFNAGRVVKNY
                     PIFPERKLAGINSIVIGSRLNGYKAWHPYYNFPNASFELVYGGLGNKKVLGNFSALLY
                     RLEFSYKMNDKFYTEITPSFGFAYFNKPYHAINNPENTLIGSAFTFCAAARLNLRYYI
                     NPFWSVSAGAGVYHCSNSHYQLPNLGINLPVASAGLRYHIRPLNLLFGNRSLEKDANY
                     HFQVRLAVGYNEQGASTFPVNGPRYPIYLGAFYVTRYLTPVNKLHLGVEGWYNSGVYD
                     FITSQRYYKDKEHLRSTALQAVVGNEFLFGNFSLLINGGIYFYNPVYHEKLRRENETD
                     FKKQLKGWITARIGFQYYLKDATIYHKNNFYAGVYIKTNLGQADFLETGIGYAF"
     assembly_gap    2512..2524
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2533..3456
                     /locus_tag="PK134_00375"
     CDS             2533..3456
                     /locus_tag="PK134_00375"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRB37339.1"
                     /translation="MFGIEYPIVQAGMIWCSGWRLASAVSNAGGLGLIGAGSMYPDIL
                     KEHIDKCKAATNKPYGVNVPLLYPDIEKLMAIIIAGGVKIVFTSAGNPATWTTHLKQA
                     GITVVHVVSNVKFALKSQAAGVDAVVAEGFEAGGHNGREETTTLCLIPEVKDALQIPV
                     IAAGGIGCGRAMMAAFALGAEGVQVGSRFVASIESSAHTNFKEKVINTSEGQTVLTLK
                     QLTPVRLIKNKFSEQVDLAQQRCATREELTQLLGRARAKKGMFEGDLDEGELEIGQVS
                     SQINNIIPAGDIVKNIWNEFLIEKNRIANLF"
     gene            3460..4707
                     /locus_tag="PK134_00380"
     CDS             3460..4707
                     /locus_tag="PK134_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB37340.1"
                     /translation="MVKFEKIILPNGLPVIVNYDPSTPLVAMNILYKVGARNEDAGRT
                     GFAHLFEHLMFGGSVNIPSYDEPLQRAGGENNAFTNNDFTNYYLTIPSQNVETAFWLE
                     SDRMLSLAFSEKSLDVQRNVVIEEFKQRYLNQPYGDVWLNLRPLAYKVHPYQWATIGK
                     DIEHIEDATLDDVKAFFHKWYCPNNAILTLSGNIKPELAFELAEKWFGEINAHELSKE
                     SLPKEPIQREYRNIMLERDIPANAVYKAFHTCSRFDNEFYATELLTDILCGSKSAVLY
                     HSLVKEQQLFNSINAYFTGDLDNGLMIVEGKVNDGVDVKHADHAIDTLLQLVVNDGVQ
                     EKEMKIVKQKTESTLVFSEVNIGNRALNLAYFEMIGSAEKINNQLQQYEVVTANNVQQ
                     VAKTIFKKENESSIYYCKKGVAV"
     gene            4704..5972
                     /locus_tag="PK134_00385"
     CDS             4704..5972
                     /locus_tag="PK134_00385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB37341.1"
                     /translation="MNRVIEPELKLTNKVDFTLPEKIILDNGIQAYVLNAGEQEVAKL
                     ELVFMAGEWYGEFSMLASAVNELLESGTAKRTAYEISQSFDQYGAYLNTSCSFDTASI
                     KLFTLSRFIPETFSLLLEIMSEAVFPDEEVKIFTDQSIQQLKINSQKVDYISRKTFNQ
                     MLFPDNHPYGRPASEADYKKLNSADLVLWHKQYYQPQNCVIFLSGRFNEDAVKSLNQL
                     FGPWKDSTLSKIVEKNFSIEKYKPERKYLEKKDALQTAIRIGCHTPNKLHPDYIPLTV
                     LSTVLGGYFGSRLMSNLREERGYTYGVGCAMVSWLHGGYFFISTQVGKQHRENALEQI
                     YLEIKRLREEKIPDNELNRVKSYMTGVYQRSLDGPMLVSDRIKSMVLYNQDFSLLNSY
                     LQIMNSTDSNQLLDLANRYFSNKMTEVSAG"
     gene            6089..13033
                     /locus_tag="PK134_00390"
     CDS             6089..13033
                     /locus_tag="PK134_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB37342.1"
                     /translation="MIRKHIIALLMFCTVFTVDVKASHLFGGEITWECIKSGPNIGKF
                     QFTLKVYRDCNGIPFTPPSAINVYGNPLVTSIPINAALTVITDLSPTGCGFTCANPAA
                     GATQEFVMKTNPVFLNGTPPASGWVFAIDDCCRNTLVNVGGGGQGFTIRAVMYPFNGQ
                     NMNPCYDSSPFFKERPNTYICIGYQYTYNPTAVDPELDSLYSDWTNPLEQPTSSPPAA
                     FNAPIINFTAPYTVNSQLPGNVTMNHNSGEVTFFVTTASSVTGNFATCLKVTAYKCQQ
                     KVAEIFRDYGVVLTTNCFVQNPPAINLPPAINLPFAGGTMKDTTVHVGDTVKFRIDAT
                     DFQRNIGSGAAFQTIRIDAYGDEFGVPINTNSGCGYPPCAKLDKLTPVISPLAAFINF
                     EWVTTCDHIIKPSGCLRERNTYYFIIKAQDNYCPAPAISTITIAITVVQSKPLEPPHV
                     RGASVLNTTGDVGLYWETPGVVNQLDTHHVFNSYQIYASNSYGGPYTLVDSVAGNKDF
                     YKQKGDTITATQLNTLIGANANAAPVYFYVRTKSLCNGDSISVPSDTISTMFVSTSIV
                     NCETQVNWNSLFKHAYPGSAALVYKIYREHPIGSPPVQIATVPNTWPNATYTDPFSKT
                     VCRDSVKYYVTIDDTLIIKSTPTVTRCVSKSNVTKQYIDNSFTASISPIGPTNLCPPN
                     CVNLTVSANPSPACFTTSYLWSNGATTSGITACAAGNYSAVITQSPSGCTSTTPIHVV
                     TLTPGPTANIAISGVSSVCPGDSVNLQFTFAGGAPYTYSYNVPGPGCVGTTVVNAVAN
                     SSPVTVRVPVNCNFNYTLNSVMGACAGTVSGSANATIKALPTASVVNTANDTVCNGGG
                     STISLAFTGTGPWNYVIKSSTGPDITATTASNPVSINVTPASTTTYTLFSVKDACTGT
                     VSGTRKIVVLPIPTAAMSITGNDTICSGSSTTIRVDFTGTGPFVGNIKDNTTGVLTPV
                     NTAASFVTLNVTPNATTTYSFQNFVSSAKACPGTIANSVVVAVKAIPTANISSVTNAT
                     ICKNDSVRMQVSFTGNGTPYTFGLVTNAGAPANVNAATNPYYFYVHPTTSTNYIVTTV
                     TDAFCSKSNINDTVAVTVNQLPTAVLSGTTTICAGQPTTLTINFTGTGPFTGSYVAST
                     GGTTNFNTSANPYTFNVSPSATTTYSLSATINDSKCSNNTNPATAIVTVNALPQASIS
                     GNKTICTGANDTLLVNFTSGNAPWTFYYKDGLGNTYGPVTTSNNPYKVVVSPTATTTF
                     QLDSVFSGVCKGSVNGIGSVTVRPLPTATFTTNNDTICNGNATNLTIQFTGTAPFSYQ
                     LQGQAVQNAATNPVTINVNPTTTTNYTLVQVNDQLCTSATSQQVKITVLNLPTAVLTT
                     TTPNLCTGVSGSITINFTGNGPFTTAYSNGTVNTPVVSNTNSIVIPVSPTVNTTYSLV
                     GNVNSMYGCNAPVNPATALITVRPLPTMTISGNPIICESTSANFNITFTGTAPFNYSY
                     FNGTSNVNGTTSTNPLTITVSPPVGTTTYTPVSIADQYCTGTALNGSSVVKVNPKPTA
                     TLFGNDTICVGSSSFIAIKFVGTPPFDYSYTTNGVSSGSLSSPVDSVSIPVAPNVNTT
                     YALSTSISDANCSNSNINQSVYMKVTPIPTAAITGTNAICSGGSTNLTINFTGEAPYN
                     YSYLAGGTTMGPFTTNNTSVTIPVSPTANTNYTLPATVNGNGCNGPASGNAMITVNAI
                     PQAVISTAKDSICKGDSTQLSIQFTGAAPFTYQLQGQGVTNATSNPHTFYVSPQSNTS
                     YVLTNITDNNCPAVINQNIGVKVLDNPTVAISTATPSLCKGNPGSITLNFTANGPYSV
                     TYNDGTSNFPVTSTSNSMTIPVTPTATTTYTLSGVVTGLLGCKTSATGATTVTVHELP
                     QAVISGNPVICLNEIAPIQIQFTGTAPFNYSYQNNVTGQINNGTTSQNPLTINLSPTT
                     TTSYNLTAISDKNCPGTQISGLANITVNPLPQPVITGVNQVCEGTPSVLSTTLPYQSY
                     QWSNATTGSTTAVTTTGTYTVEVTDINGCKKLSPPFAFIAHPYPVVNFTNDTSKTCQV
                     PRVNYFNQSLYQPGATFQWSLGENAVSNEVNPSLVYSQPGTYNVQLIVTNPFGCKDSI
                     EKQVEVIFYPLPVAQFTANPMVTNIFNGPVSFTDQSAYAVSWKWVFGEGDTLYDQNVT
                     HYFPEIGEFPVKLMIENISGCKDETQQTIVINPFWLPNAFTPNGDGRNEVFFDPGFAM
                     DYSGFNLRIFNRWGQMLFQSDSPSKPWDGSNGKGDLAPQGTYVYTLSVTNKGGKRHDF
                     NGTITLLR"
     gene            13255..14049
                     /locus_tag="PK134_00395"
     CDS             13255..14049
                     /locus_tag="PK134_00395"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRB37343.1"
                     /translation="MNQYHELLRHVMTNGVEKSDRTGTGTKSVFGYQMRFDLQKGFPL
                     ITTKKLHTKSIIHELLWFLKGETNIEYLKQNGVSIWDEWADKNGNLGPVYGHQWRSWS
                     TPDGNGIDQISSLIDTIKNNPDSRRMLVSAWNVADVSKMALPPCHILFQFYVANKKLS
                     CQLYQRSADIFLGVPFNIASYALLVHMIAQVCQLEVGEFIHTLGDAHIYSNHFEQVEL
                     QLSRTPYQLPTLQMNPLVKDVFKFKFEDFYFSDYQSHPAIKAQVAV"
     gene            14054..14557
                     /locus_tag="PK134_00400"
     CDS             14054..14557
                     /locus_tag="PK134_00400"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRB37344.1"
                     /translation="MLVSIIAAMDQNRLIGAENDLPWHLPVDMKYFKETTLGHYVLTG
                     RKNYESIPEKFRPLKGRTNLIVSNSKIQFPGAILVSSIKEGIEIARKAGEKELFIIGG
                     GQVFKQSIHLANRLYVTLIHHQFNGDVFFPQINWEQWEIISRKDVVPDENNKYACTFI
                     VAERISV"
     gene            complement(14568..15437)
                     /locus_tag="PK134_00405"
     CDS             complement(14568..15437)
                     /locus_tag="PK134_00405"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB37345.1"
                     /translation="MANATTGAGGFLGKALTALLKKNNFEVVSISRSSGVDITKAETL
                     TTIPDFDCLIHLAAHTFVPDSYNNTAAFFSINLNGTLNALELCKKRNARFIFSGSYVY
                     GQPNYLPVDENHPINMWNPYASSKIICEQLCHVYNKEFQVPVDILRIFNIYGPGQNSS
                     FLIPKIIEGVFKGQLNLETLTPRRDFIYVNDVCNAFLQCLLIHSEEQTFNVYNIGSGS
                     SYSVQQIVDKVISFTGLNPTISCNEKRRAVEVEDVKADNSKIKRDKKWHPSVSFDQGL
                     KDMIEIYRNENDL"
     gene            complement(15430..16134)
                     /locus_tag="PK134_00410"
     CDS             complement(15430..16134)
                     /locus_tag="PK134_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB37346.1"
                     /translation="MIAVLLAGGKGTRLAPFTISLPKPLMPVGNRPILEILIKQLKDA
                     GVTKVVICVGYLESLIRSYFGDGSKFGVEIIYSSETEPLGTAGPMKLIESYLTDSFFF
                     MNGDLFTDINLEEMFRFHKDNKAVATIGLTRRTVDIDFGVIKIDQTNEFTEWKEKPTL
                     EYLVSMGIYVFEPSVLKYLPDGFFNVPDLIIKLHEAKESVKGYQYKGNWLDIGRLEDY
                     QRACQQMEAIEKKSNG"
     gene            complement(16144..17118)
                     /locus_tag="PK134_00415"
     CDS             complement(16144..17118)
                     /locus_tag="PK134_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HRB37347.1"
                     /translation="MKNVLITGAGGFIGSHLTEQCVEAGYNVKAFVRYNSKSNYGWLE
                     GSKYASQIEFITGDIRDYDSVHHAVKGCDTVFHLAALIGIPYSYVSPMAYIKTNVEGT
                     YNIVQSCREHGTENVLVTSTSETYGTAQYIPIDEKHPLVGQSPYSASKIGADQIALSY
                     FLSFNTPVKIVRPFNTYGPRQSARAVIPTIITQLLSGKSDIKLGHITPTRDLTFVKDT
                     ANGFITIAQSSKFTGSVTNIGMNNEISVGDLAKLIAEMMGKKINIITDAQRVRPDNSE
                     VERLYCDNTKLITNSNWKPAYNIQSGLKETIDFLEKHLHLYKPDIYNV"
     gene            complement(17130..18452)
                     /locus_tag="PK134_00420"
     CDS             complement(17130..18452)
                     /locus_tag="PK134_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37348.1"
                     /translation="MLDFYLTIASYLGLAVIVIVGLFFNRHFFLTLFIVKPFIDMTVN
                     VSVIGDFNALEVSGVFSALILLIKYLNKPIAYPTFNEIWIWIFIGLQLLAFFLNVSAD
                     TRSIFISTKVYLKMLNSYLVYFLAAIEIFENFKQRVNLVKIVWLTALFAGLITILVFA
                     LGLSNFDTTKGVVRYNGLYNDPGTPSYLSVICLLFCNLYFKIAQKKPNHFFQFLRILT
                     LAVTAVILVITMTKSALLMFLVYVIMWYGIYERHLFLIVPALLISIYFSFSLSDDLNT
                     RFETEINYVDSGGDAEAAKSMGTGRVNRWETLLDVYFNELGPMNQLFGTSKHWMAHNQ
                     YIAYLMQVGIVGLTVFLIFLIRFIVKLYSIYHRTRNPSVYAAFTLLIMYVFYAFTGHP
                     FDYTTLLWYLMILLSMINVYEVHQMRNKQQKLKAITDKNFLLNKTQLN"
     gene            complement(18445..19590)
                     /locus_tag="PK134_00425"
     CDS             complement(18445..19590)
                     /locus_tag="PK134_00425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB37349.1"
                     /translation="MREVKKVLIVTNVPQDYRIAMFNALNLLLQKENIQFKVAFGAAG
                     YKRRKSKLDFNKMKFDYTILESTKFTFGSTENTMFTYPGLLKLHKEYRPDMTFVIGYS
                     PATIKLWFYSFFMNINYFIWSGSVNFKGRKDSTLRKILRKILVKRAKGFIAYGTKAKE
                     YLVYLGAPENKIHIAINTVDTHFFNEIAKKRLNRKVNTQKQLLYLGYLTPGKKIDDLI
                     KTINELKNLRNDFVLRIIGDGPAIENLKSLTSELHLTDIIKFEGFKQKNDLPPFMEQT
                     DCFLFQTGCDIWGLVLNEAMAAGLPCIVSPNAGAARDLVFDTKNGLILDFGSHKIAAS
                     RINELLNDEVLLQKMGYSANEFIESNVTAEKSAQGFISAIKQYCSHA"
     gene            complement(19583..21478)
                     /gene="asnB"
                     /locus_tag="PK134_00430"
     CDS             complement(19583..21478)
                     /gene="asnB"
                     /locus_tag="PK134_00430"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB37350.1"
                     /translation="MCGINGVFNFNKVNIGDENALIGLMNDTIRHRGPDDSGISEVNQ
                     RLFFGHRRLSIIDLSKAGHQPMRNEVGYEIVFNGEIYNYAELKKYLPDYKFKSDSDTE
                     VLLGLYEKFGESCLQYLNGMFAFAIWDPRKEQLFLARDRSGKKPLYYSFQNGVFAFSS
                     EVKALLKLPFIKTELDEEAFYHFLTFNVLAPPMTMFRNIKKFHPAHSMTIEKTGHFRY
                     QPYWNIEYSDYSNLTEEELKTQTLERLQKSVQYRMVSNVPVGAFLSGGVDSSALVAMM
                     SKMTNQPVKTYSIGFEGQPDFDELEHARKVSEMFGTQHFEKTVNPDEFKNFLPKIVDI
                     FDEPLADPTCVPIYFISQKARQEGTIVVLTGDGSDEIFAGYRNHLKYIHNYKTFDKFS
                     KLPTSIKKLVSGTFSLINSNSPLSEMLSRAAQNQEFYWSSARSFKESVKSRFLHPDFR
                     NRHQSLNSYSIIKAFKEEYNAIKKPFHDHIDWMCFMGFKLTDPHWYLYRGDKLGMAHS
                     IELRSPFLDYEFVNFALSIPGSMKVKNNEPKYILKKSLEAILPDEVLYRQKMGFCLPL
                     KQWGGEIMIDYVESNYVEFCKNFNLLNQDGIKQILNNVKGGNERSVNDLWTAYFLISW
                     FKRWMHA"
     gene            complement(21479..>22411)
                     /locus_tag="PK134_00435"
     CDS             complement(21479..>22411)
                     /locus_tag="PK134_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB37351.1"
                     /translation="IKGGKVYSFQAAMALAMKYNVIIDENAIKHDNQTILKYFLKHRA
                     NTPKADVTVKEIYPVVVGAVNKKSKNVALIHHIDQVHGGQSFGHKVFFMELFRKLLKM
                     DLVITVSEFWKDYLEKKGCKNVKVIYNSFIPEDYMIQKEAVAEFRKKYSIPDKPIVYI
                     GNAHKQKGVYEAYHALKDLDVELIMTGAENNASDLPIRFLSLNREEYITLLHASAIIV
                     LMSKLMEGWNRVAHEALLCNTPVVGSGMGGMGELLTKSKQIITTNDAIKENVVAVLKN
                     RNKYSNVGYDFVKQFDMNYFNSAWQQTIENLIRR"
BASE COUNT         7244 a         3914 c         4071 g         7169 t
ORIGIN      
        1 tgtatatgtt gcaaggaact attcaggagc ctggtggaac atctcaggca ttgtggggct
       61 attcagtttt taaaaatgga aatgatgttg caggcaaaac aggaactaca tccaattatt
      121 ccgatgcttg gtatatgggt gttactcatg atttggttac cggtgtttgg gtaggtgctc
      181 catatagaag cgtgcatttt cgaaattcat caggtcaggg gtcacgatta gcacttccaa
      241 tattcggaaa aatgttagag aaagcttatc aggatcccaa gtcgggtgtt gttgttggaa
      301 aattcaaaaa accgaagaaa atttccaaaa caatttattg cgaaaacgat gacattgagc
      361 tcgaagcggc agattcttta ttaaatgatt cgttgataaa tacagaattg atgattcttg
      421 aaagtgactc tttaaaatca gattcattac agggaggtgg agattaaaaa gaattttatt
      481 ttagcagaaa aaatatgcgt agaaaaatag tagcaggtaa ctggaaaatg aatggcacat
      541 tggatgatgc catcaaactg acttctgaaa ttgtagaaat gtataaggac gaaattaatt
      601 ctgatgttca ggttgtatta tcaccaccat ttccgttttt aaataacgta agtaaactca
      661 ctaatagtac tgcaattaaa attgctgcac agaattgtgc agcacaggct accggtgctt
      721 ttacaggaga agtctcagct tcaatgattg cctcatgcgg tgttggctat gttattctcg
      781 gacatagtga aagaagaacc tattttaatg ataccgatga agtacttttg ttgaaagtga
      841 agcaggcatt attgaatgga cttcaggtaa tattctgtat tggtgaaaca aaaaccgaga
      901 gagaaagcaa caatcacttt gaatgtgtgg aaaaccaaat aaaaaatgta ctttcaaatc
      961 taacgtctga cgaatttgcg aagtgtatag tcgcttacga acctgtttgg gcaattggta
     1021 cgggactgac agcttcgtct gcacaggcac aggaaatgca ttattttatc agagaagttt
     1081 taaaaagaca atttggagag caagcctctg aaacttcaat tttgtatgga ggtagttgta
     1141 atgaaaacaa tgcaaaggag ctgtttgctt tgccggatgt tgatggtggg ttaattggag
     1201 gagcatctct aaaatcaaga agttttattt ccattataaa atcattttaa ctggatgatt
     1261 aattcattca attgtgtttt actttttgct ttcttgtttt gtggtttgtt ttctgatgca
     1321 caatcattaa ggcaacctgc atttgttgaa tatagtttta atgcaggtag agtagtgaaa
     1381 aattatccta tatttcctga aagaaaattg gcaggtatca acagtattgt gattggcagc
     1441 agattaaatg gctataaggc atggcatcct tattataact tccctaatgc atctttcgaa
     1501 cttgtttatg gtggtttagg taataaaaaa gttcttggta atttctctgc attgctttac
     1561 aggttagaat tcagttataa gatgaatgat aaattttata ctgaaattac accatcattt
     1621 ggtttcgcat attttaataa gccatatcat gccatcaata atcctgaaaa cacactcata
     1681 ggttcagcat ttacattttg tgcagcagca cgactcaacc tacgatatta tatcaatcca
     1741 ttttggtctg tcagtgcagg tgccggtgtt tatcattgct ccaattcaca ttatcaattg
     1801 cctaatcttg gaattaattt accggttgca tctgcaggac ttcgttatca tatccgacca
     1861 cttaatctac tctttggtaa tcgtagttta gaaaaggatg ccaactatca ttttcaggtg
     1921 cgattagcag ttggttacaa cgagcaaggg gcaagcacat ttccggtaaa tggtccaaga
     1981 tatccaattt atctcggagc attttatgtg actcgttatc tgactccggt aaataaattg
     2041 catttgggtg tagaagggtg gtacaacagt ggcgtttatg attttattac gagccagcgt
     2101 tattataagg ataaagagca tttgcgttca acagcattgc aggcagttgt tggtaatgaa
     2161 tttttattcg gtaattttag tttgctgatt aacggtggta tatatttcta taatccggtt
     2221 tatcatgaaa agttaaggag agaaaacgaa acagatttca aaaaacaatt aaagggttgg
     2281 atcactgcaa gaataggttt tcaatattat ctgaaagatg ctaccatata tcacaagaat
     2341 aatttttatg ccggtgttta tattaagacc aatcttggtc aagccgattt tcttgaaaca
     2401 ggaatagggt atgcttttta gtttttaaga tgcaacttga ataactttaa aaatacagac
     2461 agattatcag taaaaatatt ttgtatagtt gcatcagaac agaaagaatg annnnnnnnn
     2521 nnnnttactg aattgtttgg aatagaatat ccaattgtac aagcaggaat gatttggtgc
     2581 agtggatgga gattggcttc tgcagttagc aatgcaggtg ggcttggatt aattggtgca
     2641 ggcagtatgt atcctgatat tttaaaggag catattgata aatgtaaggc tgccacaaat
     2701 aaaccctatg gtgtaaatgt tcctttgttg tatcctgata tagagaaact gatggctatt
     2761 atcattgcag gtggagtgaa aattgttttc acttctgctg gtaatcctgc aacatggact
     2821 actcatttaa aacaagcagg gattactgtt gtacatgttg taagtaatgt aaaatttgca
     2881 ttaaagtcgc aagctgccgg tgttgatgca gttgttgcag aaggttttga agccggaggg
     2941 cataatggtc gtgaagagac aacaacattg tgtttgattc ctgaggtaaa ggatgcttta
     3001 caaataccgg tgattgctgc aggtggaatt ggatgtggtc gtgctatgat ggcagctttt
     3061 gcattaggtg cagaaggtgt gcaggttggg tcgcgttttg tggcaagtat agagtcatca
     3121 gcacatacta actttaaaga aaaagttatt aatacttcag agggtcaaac agttttaaca
     3181 ttaaagcaac tgacacctgt gcgattgata aagaacaaat tcagtgaaca agttgatctg
     3241 gcacaacagc gctgtgcaac gcgtgaggaa ttgacgcagt tactcggcag ggcaagagca
     3301 aaaaaaggta tgtttgaagg agatttggac gaaggcgaat tagaaattgg tcaggtatcg
     3361 tcacaaataa ataatattat tcctgccggt gacatagtga aaaatatttg gaatgaattt
     3421 ttaatagaaa aaaaccgtat tgctaattta ttttaattta tggtgaaatt tgaaaaaata
     3481 attttgccca atggtttacc tgtgattgta aattacgatc cttcaacacc attggttgca
     3541 atgaatattt tgtacaaagt tggtgcacgc aatgaagatg caggcagaac cggttttgct
     3601 catttgtttg aacatttaat gtttggaggt tctgtaaata ttccatcgta tgatgaacca
     3661 ttgcagcgtg ccggaggtga gaataatgct tttacaaata atgattttac taactactat
     3721 ctgactattc cttcacaaaa tgttgaaaca gcattctggt tagagagtga ccgtatgtta
     3781 agtcttgcct tctcagaaaa aagtttagat gtgcaacgta atgtggtaat tgaagaattt
     3841 aaacaacgct acctgaatca accttatggt gatgtgtggc tcaatctcag gccacttgca
     3901 tataaggtgc atccttatca atgggcaaca attggtaaag acatcgaaca tattgaagat
     3961 gcaacattgg atgatgtaaa agcatttttt cataagtggt attgtcccaa taacgctata
     4021 cttacattat cgggaaacat aaaacctgaa cttgcatttg aactagcaga aaaatggttt
     4081 ggtgaaataa atgcacatga attatctaag gagagtttac caaaagaacc aatacagaga
     4141 gaatacagaa atattatgct tgaaagagac attcctgcaa atgcagttta taaagctttc
     4201 catacttgtt cacgatttga taacgagttt tatgctactg aattacttac agacatactc
     4261 tgtggaagca aatcagccgt gttgtatcac tctttagtta aagagcaaca actatttaat
     4321 tcaatcaatg cctattttac aggagacttg gataatggat taatgatagt tgaaggaaaa
     4381 gttaatgatg gtgttgatgt aaagcatgct gatcatgcta ttgatacttt gttgcaactt
     4441 gtagtgaatg atggagtgca agagaaagaa atgaaaatag taaaacaaaa aacagaatct
     4501 actttagttt tttcagaagt aaatattggt aacagggcat taaatcttgc ttattttgag
     4561 atgataggaa gtgcagagaa aataaataat cagttacaac aatacgaagt tgttactgca
     4621 aataatgttc agcaggtagc caaaacaatt tttaaaaaag aaaatgagtc atcaatttac
     4681 tattgcaaga aaggagttgc agtatgaata gagtaattga acctgaattg aaattaacga
     4741 ataaagttga ttttacatta cctgaaaaaa ttatcttgga caatggcata caagcatacg
     4801 ttcttaatgc aggtgagcag gaagttgcaa aactcgaatt ggtttttatg gcaggggagt
     4861 ggtatggtga gtttagtatg ttggcctctg cagtaaatga gttgttggaa tccggaacag
     4921 caaagcgtac agcctatgaa atttcacagt cattcgatca atatggagcc tatctcaaca
     4981 cctcttgttc atttgataca gcatcaatta aactttttac gttatcacga tttattccgg
     5041 agactttttc attgttactg gaaattatgt cagaggctgt atttcctgat gaagaagtga
     5101 aaatattcac agatcaaagt attcagcaat taaaaatcaa tagtcaaaag gttgattata
     5161 tttcaagaaa gactttcaat cagatgctat ttccggataa tcatccttat gggaggccag
     5221 catcagaagc agattataag aagttgaatt ctgctgattt ggttttatgg cataaacaat
     5281 attatcaacc tcaaaattgt gtgatttttc taagcggacg ttttaatgag gatgctgtta
     5341 aatccttaaa tcaattattt ggaccatgga aagactctac actaagtaaa atagttgaga
     5401 aaaatttttc tattgaaaaa tataagcctg aaagaaaata tcttgagaaa aaggatgcat
     5461 tacaaactgc tattcggatt ggatgtcata ccccgaataa acttcatccc gactatattc
     5521 cattgacagt tttatcaacc gttttgggcg gatattttgg ttcaaggctg atgagtaatt
     5581 taagggaaga acgaggttat acctatgggg ttggttgtgc aatggtgtcc tggcttcatg
     5641 gcggatactt cttcatttct acacaagtgg gcaaacaaca tcgtgaaaac gcgcttgaac
     5701 agatatatct tgaaattaag cgacttcgtg aagaaaaaat cccggataat gagctcaatc
     5761 gtgtaaaaag ctatatgaca ggcgtttatc aaagaagttt agatgggcca atgcttgttt
     5821 ctgatagaat aaaatctatg gttttataca atcaggattt cagtttacta aattcatatt
     5881 tacaaatcat gaattctact gattcaaacc aattacttga cttggcaaat cgttattttt
     5941 ctaataaaat gactgaggtt tctgcaggtt aagttcaatg aattttttca aacggcaact
     6001 ctgttaaaaa aatctcgttt aattgttaaa tttatgatgt agaaatgaag attagtttta
     6061 tatttgtaca aacaaattta atgaagctat gatacgaaaa cacattattg cacttttgat
     6121 gttctgtaca gtgtttacag tagatgtcaa agcaagtcac ctttttggtg gtgagattac
     6181 ttgggaatgt attaaatcag ggcccaatat tggtaaattt cagtttacct taaaagttta
     6241 cagagattgt aatggtattc cttttacgcc cccatcagca attaatgtat atggaaatcc
     6301 attggttact tccattccaa ttaatgctgc attaactgta ataactgact taagtcccac
     6361 aggttgtggt tttacttgtg caaatcctgc agctggtgca actcaagaat ttgtaatgaa
     6421 aacaaatccg gtgtttttaa atggaactcc tcctgcttca ggatgggttt ttgcaattga
     6481 cgattgttgc agaaatactt tggttaatgt tggtggaggt ggacaaggtt ttacgatcag
     6541 agctgttatg tatccgttta atggacaaaa catgaatcct tgttatgatt cttctccatt
     6601 ttttaaagaa agacctaata catatatctg tatcggatat caatatactt acaatccaac
     6661 tgccgtagat cccgaattag attcacttta tagtgattgg actaatccat tagagcaacc
     6721 aacaagttct cctcctgccg ccttcaatgc tccaataatt aattttactg caccctatac
     6781 tgttaacagt cagttgcctg gtaacgtaac aatgaatcat aattcaggtg aagtaacttt
     6841 ttttgtaaca acagcatcaa gtgtcactgg taattttgca acttgcctga aagttactgc
     6901 ttataaatgt caacagaaag ttgcagagat tttcagagat tatggtgtag tgcttactac
     6961 taattgtttt gtacaaaatc caccggcaat taatttgcca ccggcaatta atttaccttt
     7021 tgccggagga actatgaaag atactactgt tcatgttggt gatacggtca aattcaggat
     7081 tgatgctaca gactttcaac ggaacattgg ttcaggagct gcatttcaga caattcgtat
     7141 agatgcttat ggcgatgagt ttggtgtgcc tataaataca aacagtggat gtggttaccc
     7201 accttgtgcc aagttagata aattgacccc tgtaatttca cctttagctg cctttataaa
     7261 ttttgaatgg gtcacaacat gcgatcacat cattaaaccc tcggggtgtt tacgagaaag
     7321 aaatacctac tacttcatca ttaaagcgca ggataattat tgtccggcac cggcaatcag
     7381 tactataacg attgcaatta ctgttgttca atctaaaccc ttggagccac ctcatgttcg
     7441 gggtgcatcg gtgcttaata caaccggtga tgttggatta tattgggaaa caccgggtgt
     7501 cgtcaaccaa ctcgatacgc atcatgtatt caattcctat cagatttatg catcaaatag
     7561 ttatggtggc ccatacactt tagtagattc tgttgcagga aataaggatt tctataaaca
     7621 gaaaggtgat actatcactg ccacacaact aaatacattg attggtgcca acgcaaacgc
     7681 tgcacctgtt tatttctatg taagaacaaa atcattgtgt aatggggata gtatttctgt
     7741 tccttcagat acaattagta caatgtttgt cagtacaagt attgtgaatt gtgaaacaca
     7801 agtaaattgg aattcgcttt ttaaacatgc atatccgggt tctgcagctt tagtctataa
     7861 aatttatcga gaacatccta taggttctcc accagtccaa attgcaacag tgccgaatac
     7921 ctggccaaat gcaacatata ccgatccatt ttcaaagaca gtatgtcgtg acagcgtgaa
     7981 gtattatgtc actattgatg atactttaat tataaaatca accccaacag ttacacggtg
     8041 tgtttcaaaa tctaatgtta ccaagcaata tattgataat agtttcactg ctagtatttc
     8101 acctataggt ccaacaaatc tttgtccgcc aaattgtgta aatttaaccg tttcggccaa
     8161 tcctagtcct gcttgtttta ccacttcata tttatggagt aatggagcaa ctacttctgg
     8221 tattacagct tgtgcagcag gaaactattc agcagttata acgcaaagtc caagcggatg
     8281 tacaagtaca acaccaatac acgttgtaac attaacacct ggtccaactg caaatattgc
     8341 aatttcaggt gtaagtagtg tttgccccgg tgatagtgtg aatcttcagt ttacatttgc
     8401 aggtggtgct ccttatacat atagttataa tgtacccggc ccgggttgtg ttggaacaac
     8461 tgttgttaat gcagtagcca attcatcacc ggttacagtc agagtacctg ttaattgtaa
     8521 ttttaactat acactcaatt ctgtaatggg tgcttgtgca ggaacagtaa gtggaagtgc
     8581 aaatgcaaca ataaaagcat tgccaactgc aagtgttgta aatactgcaa atgacactgt
     8641 atgcaatgga ggtggttcca ctatttcact tgcatttaca ggtactggtc cttggaatta
     8701 tgttattaaa agcagtacgg gtccagatat tacagcaaca acagcttcaa accctgtttc
     8761 aataaatgtt actcctgctt ccacaacaac ctatacattg tttagtgtta aagatgcatg
     8821 tacaggaacc gtttccggaa ccagaaaaat tgttgttctt ccaataccaa ctgctgctat
     8881 gtctattacc ggaaacgata ctatctgtag cggcagctca actacaattc gtgttgactt
     8941 tacaggcaca ggcccttttg ttggaaatat taaagataat acaacaggtg ttttaacacc
     9001 ggtaaataca gcagcatctt ttgtgacact caatgtgact ccgaatgcaa caactactta
     9061 ttcattccag aattttgtta gctctgctaa ggcttgcccc ggtacaattg ctaatagtgt
     9121 tgtggttgct gtaaaagcaa ttcctacagc aaacatttca agtgttacca atgccacaat
     9181 ctgtaaaaat gacagcgtgc gcatgcaggt tagttttaca ggcaatggaa cgccatacac
     9241 atttggtttg gtgaccaatg ccggtgcacc tgcaaatgta aatgcagcta cgaatcccta
     9301 ttatttttat gtgcatccta caacgagtac taattatata gttacaacag ttactgatgc
     9361 attttgcagt aagtcaaata tcaatgatac agtagctgta acagtaaacc aactgcctac
     9421 tgccgtactt tccggaacta caacaatttg tgccggtcag ccaacaactt tgacaattaa
     9481 ctttaccgga acaggtccat ttacaggaag ctatgttgca agtacaggtg gcaccaccaa
     9541 ttttaatact tcagcaaatc cttacacatt taatgtttcg ccaagtgcaa caacaacgta
     9601 ttcattaagt gctaccatta atgattcaaa atgttctaat aacaccaatc ctgcaacagc
     9661 aattgtaacg gttaacgcat tgccgcaggc atctatcagc ggcaacaaaa caatctgtac
     9721 cggagcgaat gatactttat tagtaaactt tacttcaggt aatgcaccat ggacattcta
     9781 ttacaaagat ggattaggaa atacttatgg tccggttaca acaagtaata acccatataa
     9841 ggtagttgtt agtccaacag ctactactac atttcaactt gattctgtat ttagcggagt
     9901 ttgtaaagga agtgtaaatg gaattggtag tgttactgtg cgaccgttgc caacagcaac
     9961 atttacaact aacaacgata ctatttgtaa tggtaatgca actaacttaa caattcagtt
    10021 taccggtact gcaccattca gttatcagtt acaaggacaa gcagtgcaga atgcagctac
    10081 caaccctgtt accatcaatg ttaatccaac aactactaca aattacacat tggttcaggt
    10141 aaatgatcag ctttgtacaa gtgcaacaag tcaacaagta aaaattacag ttcttaattt
    10201 accaactgct gttctgacaa caacaacgcc taatttatgt acaggagtga gtggaagtat
    10261 taccataaac tttacaggta acgggccttt cactacagca tactcaaatg gaactgtaaa
    10321 cacacctgtt gtaagcaata caaattctat tgttattccg gtttctccaa cagttaacac
    10381 aacatattca ttagttggaa atgttaacag catgtatggt tgtaatgcac ctgttaatcc
    10441 ggcaactgct ttaattactg tgagaccatt gccaacaatg actatcagtg gtaatccaat
    10501 aatatgtgag agtacatcag caaatttcaa cataacattt accggtacag caccattcaa
    10561 ttatagttat tttaatggaa catctaatgt aaatggaaca acaagtacaa atccattaac
    10621 aataaccgta agtccgcctg ttggaactac aacctatact cctgttagta ttgccgatca
    10681 atattgtaca ggcacagcgt tgaatggttc atctgttgtg aaagttaatc caaaaccaac
    10741 tgcaacatta tttggaaatg ataccatttg tgtaggaagc agttctttca ttgcaattaa
    10801 atttgtgggt acaccgccat tcgactatag ttatacaaca aatggcgttt catcgggtag
    10861 cctttcttcg cctgttgact ctgtttcaat tcctgttgca ccaaatgtta atacaactta
    10921 tgcattgtca acaagtattt ctgatgctaa ttgctctaac agcaatatca atcagtcggt
    10981 ttacatgaaa gttactccaa taccgacagc agcaataacg ggaaccaatg caatttgtag
    11041 tggaggttca acaaatctca ccatcaattt taccggagaa gcaccttata attattctta
    11101 cttggccggt ggaactacaa tgggaccatt tacaaccaat aatacttctg taacaattcc
    11161 tgtgagtcca actgccaata caaattatac acttccggca acagttaatg gaaatggatg
    11221 taacgggccg gcttcaggta atgcaatgat tacagtcaat gcaataccac aggcggtaat
    11281 ttctactgca aaagattcaa tctgtaaagg tgatagtaca caattatcaa ttcaatttac
    11341 aggtgctgca ccgtttacat atcagttaca gggacaaggt gttactaacg caacatctaa
    11401 tccgcataca ttctatgttt cgccacaaag caatacatct tatgtactga caaacattac
    11461 agataataat tgccctgcag taattaatca gaacattggt gttaaagtgc tggataatcc
    11521 aactgtagct atttcaacgg caacaccttc attgtgtaaa ggaaatccgg gaagtattac
    11581 tcttaacttt actgcaaatg ggccttatag tgtaacttac aatgacggca catcaaactt
    11641 ccccgtgacg tccacaagta attcaatgac aattcctgtt acgccaacgg caactactac
    11701 atatacattg tccggagtag ttacaggttt attaggctgt aagacttctg caacaggagc
    11761 aacaactgtt acagtacatg aattaccaca agctgttatt tcaggcaacc ctgtaatatg
    11821 tttaaatgaa atagcaccaa tacagattca gtttacggga acagctccat ttaattatag
    11881 ttatcagaat aacgtgacag gacagattaa caatggaaca acatcacaaa atccattaac
    11941 aataaatctt agtccaacaa ctaccacatc gtataatctg acagcaatta gtgataagaa
    12001 ttgccccgga acgcaaataa gtggtttagc aaatattaca gttaatccgt tgccgcagcc
    12061 tgtcattaca ggtgtaaatc aggtttgtga aggtacacct tctgttctat caacaacact
    12121 tccttaccaa tcttatcaat ggtctaatgc aactacagga agtacaacgg cagttactac
    12181 tacaggtaca tatacggtag aagttactga tataaatggt tgtaaaaaat tatctccacc
    12241 atttgcattt attgctcatc catatcctgt tgttaatttt acaaacgata caagcaaaac
    12301 atgtcaggtg ccaagagtta actacttcaa tcaatcattg tatcagcctg gagcaacatt
    12361 ccaatggagt cttggtgaaa atgctgtttc caatgaagtt aatccttcac ttgtttacag
    12421 tcagccgggt acttataatg tgcagttgat tgttaccaat ccatttggat gtaaagattc
    12481 aatagagaaa caagtagaag taattttcta tccattgcct gttgcacaat ttacagcaaa
    12541 tccaatggtg acaaatattt tcaacggacc agtaagtttc actgatcagt ctgcctatgc
    12601 agttagttgg aaatgggtgt ttggtgaagg agatacatta tatgatcaga atgttaccca
    12661 ttatttccca gagattggag agtttcctgt taagttgatg attgaaaaca tttctggttg
    12721 taaagatgaa acacaacaga ccattgtgat taatccattc tggttaccaa atgcatttac
    12781 accaaatggt gatggacgaa atgaagtgtt ctttgatcct ggttttgcga tggattattc
    12841 aggatttaat ttacgtattt tcaaccgttg gggacaaatg ctgttccagt cagattctcc
    12901 atcaaaacca tgggatggct caaatggcaa aggtgattta gcacctcagg gaacctatgt
    12961 ttatacactt agtgttacca ataaaggtgg aaagcgacat gatttcaatg gaacaattac
    13021 tttgttgaga taagattatg taaaaattag attgtttgat aaataacagc cgttaaaatc
    13081 aattacagat tttagcggct gtttagtttt acgaagatta aactttattg ctcacaaatt
    13141 gagcaaatat taaatgtaac attgtgaata aaaacaacct ctacggatgc atagtcagac
    13201 atgaaatcca tttgtttaat ttaattttga agccttaaaa ttgtttataa tagaatgaat
    13261 cagtatcacg agttactcag gcatgtaatg accaatggcg tggaaaaatc tgaccgcaca
    13321 ggtacaggta ctaaaagtgt ttttgggtat cagatgcgat ttgatttgca gaagggtttt
    13381 ccgcttatca caacaaaaaa acttcacacc aaatcaatca tacatgaatt attatggttc
    13441 ttgaaaggcg aaacgaatat tgaatactta aagcaaaatg gtgtttcaat atgggatgag
    13501 tgggctgata agaatgggaa tctcggtcct gtatatggac atcagtggcg ttcgtggtca
    13561 actcctgatg gtaatggaat tgatcagata agttcgttga ttgacacaat taaaaacaat
    13621 cccgactcgc gtaggatgct tgtgagtgca tggaatgttg ctgatgtatc taaaatggca
    13681 ttgccaccat gtcacatact ttttcagttt tatgtagcta ataaaaaatt gtcatgtcag
    13741 ctttatcaac gtagcgctga tatttttctt ggtgtgccct ttaacatagc ctcttatgct
    13801 ttgcttgtac acatgattgc acaggtttgt caattggaag ttggtgagtt tattcatacc
    13861 cttggcgatg cacacattta ttccaaccat tttgaacagg tagaactcca actttcgcga
    13921 acaccatatc aattgccgac actgcaaatg aatccattgg taaaagatgt ctttaagttt
    13981 aagtttgaag atttttattt ttcggactat caatcgcatc ctgcaattaa agcacaggta
    14041 gcagtttaat tttatgttag tttcaataat tgctgctatg gatcagaatc gtttaattgg
    14101 tgctgaaaat gatttgccat ggcatttgcc tgtggacatg aagtatttca aagaaactac
    14161 tttaggtcat tatgttctta caggtagaaa aaattatgag agtattcctg agaaattcag
    14221 accgctgaaa ggtagaacga atttaattgt atcgaattct aaaattcaat ttccgggagc
    14281 tattttggtt tcaagtatta aagaaggtat tgagatagca agaaaggcag gggagaagga
    14341 gctttttatt attggtggcg gacaagtgtt taaacaatca atacatcttg caaataggct
    14401 ttatgtcact ttgatacatc atcaatttaa tggagatgtt ttttttcctc aaattaattg
    14461 ggagcaatgg gaaattatat caagaaaaga tgttgtgcca gatgaaaaca ataaatatgc
    14521 ttgtactttt attgttgctg aaagaatttc agtttaatga taataagtta taaatcattt
    14581 tcattccggt aaatttcaat catatcttta agtccctgat caaatgaaac ggacggatgc
    14641 cattttttat cacgcttaat tttggagtta tctgctttta catcttcaac ttcaacggca
    14701 cgtctttttt cattacacga tatcgttgga tttaaaccag tgaaagaaat caccttgtca
    14761 actatttgtt gtacactgta gctactgcca ctaccaatat tataaacatt aaatgtttgc
    14821 tcttcagaat gaattaacaa acattgtaag aaagcattac aaacatcatt tacgtaaata
    14881 aaatctcgtc taggtgtaag tgtttcaaga tttaactggc ctttaaaaac accttcaatt
    14941 attttaggaa ttaaaaaaga agaattctga cccggaccat aaatattaaa aattcgtaaa
    15001 atgtcaacag gaacctgaaa ctccttatta taaacatgac acagttgttc acaaataatt
    15061 ttacttgatg cataaggatt ccacatgttt atagggtgat tctcgtcaac aggaaggtag
    15121 tttggttgac cataaacata cgagcctgaa aaaataaatc gtgcattgcg tttcttacat
    15181 aactcaagtg cattaagtgt tccatttaaa tttatggaga aaaatgctgc tgtattatta
    15241 tatgaatccg gaacgaaagt atgagctgca aggtgtatga ggcaatcgaa atcgggaatt
    15301 gtagttaatg tttctgcttt tgtaatatca acaccggagg atcttgaaat actaactact
    15361 tcaaaattat tcttttttaa taaagcggtt aatgctttac caagaaaccc accggcaccg
    15421 gttgtagcat tagccatttg attttttctc tattgcttcc atctgttggc aggcacgttg
    15481 gtaatcttcc aagcggccaa tgtctaacca atttccttta tattgatatc cctttacact
    15541 ttcctttgcc tcatgcagtt ttataatcaa atcaggaacg ttaaagaagc catcaggcaa
    15601 atattttaac acgctaggtt caaaaacata gatccccata cttactaaat attcaagagt
    15661 gggtttttct ttccattcag taaattcatt agtctgatca attttaatta ctccaaaatc
    15721 aatatcaact gttctgcgag taagaccaat agtagcaacg gctttattgt ctttatgaaa
    15781 tctgaacatt tcttcaagat taatgtcggt aaacaaatca ccattcatga aaaagaatga
    15841 gtctgttaaa tatgattcaa taagtttcat tggacctgct gtacctaaag gttctgtttc
    15901 acttgagtag attatttcaa cgccaaactt tgagccatca ccaaaataag aacgtattaa
    15961 tgattcaagg taaccaacac aaataacaac cttagtcaca cctgcatctt taagttgttt
    16021 gattaatatt tctaaaatag gtctgttgcc aacaggcatt aaaggctttg gtaaactaat
    16081 agtaaaaggc gctaagcgtg tgccttttcc tcctgctaat aaaactgcta tcatatcttg
    16141 aatttaaacg ttgtaaatgt caggtttgta aagatgcaaa tgcttttcga gaaagtcaat
    16201 agtttctttt aagcctgatt gaatattgta tgccggtttc cagttactat tggtaatgag
    16261 tttggtattg tcgcagtaaa gacgctctac ttcactgtta tcaggacgaa cacgctgagc
    16321 atcggtaata atatttattt tctttcccat catttctgca attagtttag ccaaatcacc
    16381 aacactgatt tcattgttca tgccaatatt agtaacagat ccggtaaatt tggatgattg
    16441 ggctattgtg ataaatccgt ttgcagtgtc tttaacaaat gtaaggtctc ttgtaggagt
    16501 gatgtgccct aacttaatat cacttttacc acttaataat tgcgtgatga ttgtaggaat
    16561 tacagctctt gcactttgac gtgggccata agtattaaag ggtcttacaa tttttacagg
    16621 cgtgttgaat gaaagaaagt aacttaatgc aatttggtcg gcaccaattt ttgaagcaga
    16681 gtagggtgac tgtccaacca atgggtgttt ttcatcaatt ggaatatatt gtgcagtgcc
    16741 ataagtttca ctcgtagatg ttacaagaac attttctgtg ccatgttcac ggcaagattg
    16801 aacaatatta tacgtgccct caacatttgt ttttatatag gccattggag aaacatagct
    16861 gtaaggaatg ccaattaatg ctgctaagtg aaagacagta tcgcagcctt taacagcatg
    16921 atgtacactg tcatagtctc tgatatcgcc agttataaat tcaatttgac tggcatattt
    16981 agagccttca agccagccat aatttgactt tgaattatat ctgacaaatg ccttgacatt
    17041 atatccggct tcaacacatt gttctgtgag atgtgaaccg ataaatcctc ctgcacctgt
    17101 aatgagtaca tttttcatct gatattttat taatttagtt gtgttttgtt aagtaagaaa
    17161 tttttatctg ttatcgcttt cagtttttgt tgtttgtttc gcatctgatg tacttcatat
    17221 acattaatca tagaaagtag aatcattaaa taccaaagta atgtagtata atcaaatgga
    17281 tgaccagtaa aggcataaaa tacatacatg attaaaagtg taaatgcagc ataaacagaa
    17341 ggattgcgtg tgcggtggta aatactgtat agttttacta taaaccgtat tagaaatatt
    17401 agaaagactg ttaaaccaac aatgccgacc tgcattaaat aggcgatata ctgattgtgt
    17461 gccatccaat gtttggatgt tccgaaaagt tggttcattg gccccaattc attaaaataa
    17521 acatcaagta aagtttccca acggttaact ctacctgtgc ccattgattt ggctgcttcg
    17581 gcatctccac ctgaatccac atagttaatt tctgtttcaa atcgggtatt caggtcatca
    17641 gataagctga acgaaaaata tatagaaatt agcagcgcag gaacaattaa aaataaatgc
    17701 ctttcgtaga ttccgtacca cattataacg taaactaaaa acatgagtaa agcactttta
    17761 gtcattgtaa taacaagaat aactgcggtg actgcaagag ttagtattct taggaattga
    17821 aaaaaatgat tgggtttctt ttgtgcaatt ttaaaataca aattgcaaaa taataagcaa
    17881 attacagaaa gataggaagg ggttccagga tcattataca gtccattata tcttactaca
    17941 cctttcgtag tatcaaagtt tgataatcct aaagcaaaaa ccagaattgt aatcaatccc
    18001 gcaaatagag cagttaacca aactattttt actaaattta ctcgttgctt aaagttctca
    18061 aatatctcaa ttgctgctaa gaagtaaacc agataggagt tcagcatctt cagatatact
    18121 tttgtactta taaagatgga gcgtgtatcg gcagatacat tcaaaaagaa agcaagcaac
    18181 tgcaagccaa taaaaatcca aatccaaatt tcattaaatg taggataggc aataggctta
    18241 tttaagtact taattaataa aattaaagca ctaaacactc cggaaacttc caatgcatta
    18301 aagtctccaa ttacactgac attaactgtc atgtctataa atggtttgac tatgaatagt
    18361 gttaaaaaga aatgacgatt gaagaaaagc ccgacaatca caataacggc caagcctaaa
    18421 taagaagcaa tggttaaata aaaatcaagc atggctacaa tattgtttta ttgctgaaat
    18481 aaatccttga gcacttttct ctgctgtgac gttactttct ataaactcat tagcgctata
    18541 tcccattttt tgaagaagca cttcatcgtt taacagttcg tttattcgac ttgctgcaat
    18601 cttatgtgat ccaaaatcta aaatcagtcc attcttagtg tcaaaaacta aatcacgagc
    18661 agcacctgca ttaggcgaaa ctatacaagg tagtcctgct gccattgctt catttaaaac
    18721 caaaccccaa atgtcacagc ctgtttgaaa taaaaaacaa tctgtctgtt ccataaacgg
    18781 gggcaagtcg tttttttgtt tgaaaccctc aaatttaatg atgtctgtta aatgcaattc
    18841 agaagttagt gattttaaat tttcaattgc aggtccatca cctatgatac gtaaaacaaa
    18901 atcattgcga agatttttta attcattaat cgtcttaatg aggtcatcta ttttttttcc
    18961 gggcgttaaa taacctaaat acaatagttg tttttgagta ttaacttttc tgtttaagcg
    19021 tttctttgca atctcattaa aaaaatgtgt gtcaacggtg tttattgcta tatgtatttt
    19081 gttttcaggt gcgcctaaat aaactaaata ttcttttgct ttggtgccat aagcaataaa
    19141 cccttttgct ctcttaacta atatttttct tagtattttt ctaagagttg aatcttttcg
    19201 acctttaaaa ttaaccgagc ctgaccatat aaagtagttt atattcataa aaaacgaata
    19261 aaaccaaagt ttaatggttg cagggctata cccgataaca aatgtcatgt ccggtctata
    19321 ctctttatgt aattttaaaa gtccgggata agtgaacatg gtattctctg tagaaccaaa
    19381 tgtaaactta gttgactcaa gaattgtgta atcgaatttc attttattga aatcgagttt
    19441 ggattttctc cgtttatatc cggctgcacc gaatgcaact ttaaattgta tattctcttt
    19501 ctgcagtaat aagtttaatg cattgaacat agcaattcta taatcctgtg gcacattggt
    19561 aactataaga acttttttta cctcacgcat gcatccatct tttaaaccat gatattaaaa
    19621 aatatgctgt ccataaatca tttacagatc tttcattgcc tccttttaca ttattcaata
    19681 tttgtttgat tccgtcctga tttagtaaat taaaattttt gcaaaattct acatagtttg
    19741 attctacata atcaatcatg atttcaccac cccactgctt taaaggtaaa caaaagccca
    19801 tcttctgtct gtaaagaacc tcgtcaggca aaatggcttc gagtgacttt tttagaatat
    19861 atttcggctc attattttta actttcatac ttcctggaat agaaagagcg aagttaacaa
    19921 attcatagtc aagaaatgga gaacgaagtt ctatagaatg agccatacct aacttgtcac
    19981 cacgataaag ataccaatga gggtcagtta gtttaaaacc catgaaacac atccaatcaa
    20041 tatgatcgtg aaaaggtttt ttgatagcat tatactcctc tttaaaagct ttaatgattg
    20101 aataggaatt tagtgactga tgtctgttcc tgaaatcagg gtgcagaaac ctacttttta
    20161 ctgattcttt aaaacttctg gcactactcc aataaaattc ctgattttga gctgcacgac
    20221 ttaacatttc gcttaatgga gaattcgaat ttatcaatga aaaagttccg gaaacaagct
    20281 ttttaatgct tgtaggtaac ttactgaact tatcaaaagt tttgtagttg tgaatatatt
    20341 ttaaatggtt acgatatcct gcaaaaattt catcacttcc atcaccggtt agcacaacta
    20401 ttgtaccttc ctgtcttgct ttctgagaaa tgaaatagat gggaacgcag gtagggtcag
    20461 ccaatggttc atcaaatatg tctacaatct ttggcagaaa atttttaaat tcgtctgggt
    20521 ttactgtttt ttcaaaatgt tgtgtgccga acatttcaga aacttttctt gcatgttcaa
    20581 gttcatcaaa atcgggttga ccttcaaaac cgatagagta ggttttaaca ggctgatttg
    20641 tcatttttga catcatagcc accaatgcac ttgagtcaac accaccacta agaaatgctc
    20701 ctacaggaac attagaaacc attctatact gaacagattt ctgcaatcgt tccagagttt
    20761 gagtttttaa ctcttcttcc gtaagattgc tataatcact atattcaata ttccagtaag
    20821 gctgatatct gaaatgaccg gttttttcaa tggtcatact atgtgcagga tgaaactttt
    20881 taatgtttct gaacatagtc attggagggg ccaatacatt aaatgttaag aagtgataga
    20941 atgcttcctc atctaattct gtttttataa atggtagttt taacaatgct tttacctctg
    21001 aagagaaggc aaatacacca ttctgaaagg aataatataa aggttttttt cctgaacgat
    21061 ctcttgctaa aaataattgc tccttccggg gatcccaaat tgcaaaagca aacatcccat
    21121 tcagatactg aaggcaactt tctccaaact tttcatagag tccaagcaat acttctgtgt
    21181 cactatcaga tttaaattta taatcaggca gatatttttt caactctgca taattgtaaa
    21241 tctcaccatt gaaaactatt tcataaccga cttcatttct cataggttga tgaccggctt
    21301 ttgaaagatc tataatacta aggcgcctat gtccaaaaaa taagcgttga ttaacttcag
    21361 aaataccact atcatccgga ccacgatgcc tgatagtgtc attcatcaaa cctattagag
    21421 cgttctcgtc accgatgttg actttattaa aattaaaaac tccgttaatg ccgcacattt
    21481 accttctaat taagttttct attgtttgtt gccatgctga attgaagtaa ttcatgtcaa
    21541 actgttttac aaagtcgtaa ccaacattac tatatttatt tctattcttt aaaactgcca
    21601 caacattttc ttttatagca tcatttgttg ttattatttg tttcgatttt gtaagaagtt
    21661 cacccatgcc acccattcct gacccgacaa ctggtgtgtt gcaaagtaat gcttcgtgag
    21721 caacccggtt ccatccttcc attaactttg acatgagcac aataatcgca gaggcatgaa
    21781 gcaaggtgat atattcttct ctgtttaatg ataaaaaacg tatcggcaag tcagatgcat
    21841 tattttctgc accggtcatg attaactcaa catctaaatc ttttaatgca tgataggctt
    21901 cataaactcc tttttgttta tgagcattgc caatataaac tataggcttg tcaggaatag
    21961 aatatttttt tctgaattct gcgacagctt ctttttgtat catgtaatct tctggaataa
    22021 aagaattgta aatgacttta acatttttac accctttttt ttcaagatag tctttccaaa
    22081 attctgaaac agtaataact aaatccattt tcagaagctt cctgaaaagt tccataaaga
    22141 aaactttatg accgaatgat tgtccaccgt gaacctgatc tatatgatga atcaaggcta
    22201 cattctttga tttcttattt actgcgccaa ctactaccgg ataaatttct ttgacagtga
    22261 catcagcctt tggtgtattt gctctatgtt ttaggaaata ttttaatatt gtttgattat
    22321 cgtgctttat ggcgttttcg tcaataataa cattatactt cattgctaaa gccatggctg
    22381 cttgaaaact atatacttta ccacccttaa t
//
LOCUS       DAORQT010000005        21837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_153_length_21837_cov_14.884216, whole genome shotgun sequence.
ACCESSION   DAORQT010000005 DAORQT010000000
VERSION     DAORQT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21837
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_21837_cov_14.884216"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..2646
                     /locus_tag="PK134_00440"
     CDS             253..2646
                     /locus_tag="PK134_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRB37352.1"
                     /translation="MSNTDTVNKVKCYHCGDDCDEQVITSDEKIFCCTGCKLVYEILR
                     ENNLCNYYNLNNNPGTKQSDIVQKRFAFLDEPSIENQLISFRDKNETIITFYIPAMHC
                     SSCIWLLENFQKADEGVLSSRVDFVRKELRLVYDHHKTSLRKIAEKLSQTGYEPALQL
                     DAIEKKKIKKANRSRILRIGVAGFCSGNIMMLSFPEYFSSGNNIDASLQTLFSYLILL
                     LALPVFFYSANEFFIKSWQALKQKTTSIDLPIAIGISVIFLRSAFEIITQTGSGFFDS
                     GSGLVFFMLIGRWFQDFTFDSLAFDRDFKSYFPIAVTSIKNGNEKEVQINDLKVNDRI
                     LIRNNEIIPADCILLKGNASVDYHFVTGESLPVSHTAGEYIYAGGKQAGAMIELQVMK
                     EVSQSYLVQLWNRSWDKQDISKFEKLVNAISRWFIVVTFSISTIAAIYWWNTDIHKAI
                     NAFTAVLVIACPCALALSAPFTYGNILRILGRNKIYMRNYRVLEKIADADTIVFDKTG
                     TLTKNNVSEISYHGQSLDDEDKKILSSLFRQSSHPLSKLLQQHLQADKYYPCNNFEEV
                     TGKGICAVLNNKNIKAGTSDWAGVNNEVEKNATAVFININDIKKGKFVFSNIYRDGLN
                     TISQKLNNKHNLIVLTGDNESERKNLTTLLGNAELRFNQKPEDKRNYILELINSKKNV
                     LMIGDGLNDAGALLAANTGISVTDNTNNFTPASDTIMDAAALHQLPELIKLSKSAVTI
                     IIISFIISLLYNFVGLAFAVSGTLSPLFAAILMPISTISLVLFTVGASTIKARQSGF"
     gene            complement(2650..3783)
                     /locus_tag="PK134_00445"
     CDS             complement(2650..3783)
                     /locus_tag="PK134_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB37353.1"
                     /translation="MIINTSELSGRSIQATDKTPLRILLVDDIPANLLTLEGILEDDS
                     RILIKAFSGNEALKLLMEEPIDLILLDIQMPDMDGVEVAQLLRANPKTKHIPIIFVSA
                     IAKSERPSLEEFEIGSFDFIPKPLDIEETQKIVSIFEFGCRYKQKYREQEEKLKRLND
                     HLDKFLYILSHDLNAPLRAIENLVQWLEEDLQSQISDESKENIGLLRNRVTKMKLLLD
                     GLLRYARSGNTKHQHDIISPEKMIRQITSELQGDRKVSLNVNSSVLDMNTDRTALQLV
                     LKELIQNAISFNPSDKNTIDISVVEKNDSYEFVIADNGPGIKPQQQKTIFELFQTLPS
                     SVVPSSGVGLSIVKKVVEEYGGTVWFSPTVKCGTEVHFNWKKS"
     assembly_gap    3800..3809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3868..4743
                     /locus_tag="PK134_00450"
     CDS             3868..4743
                     /locus_tag="PK134_00450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB37354.1"
                     /translation="MELLTPQNFKDYELLDSGNFEKLERFGQYITIRPEPQAVWNRQL
                     KEADWLKKAHVKFIAKNSSSGTWKNLKEIPDRWHIAYALPDEKQIRLRLALTSFKHVG
                     VFPEQAVNWDFIYNSVKKLDVVQPKVLNLFAYTGASTLAAKAAGADIIHLDSIKQVVT
                     WAKENMTLSGLDNIRWLVEDAMTFVKREVKRGKKYQGIILDPPAYGHGPNGENWKLED
                     EINEMLANVKLLLEENHFLVLNTYSLGFSSIILDNLLDEHFSKNIKRQTGEIFLQSTA
                     GARLPLGVFARHCTV"
     gene            complement(4748..5590)
                     /gene="panC"
                     /locus_tag="PK134_00455"
     CDS             complement(4748..5590)
                     /gene="panC"
                     /locus_tag="PK134_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRB37355.1"
                     /translation="MKLIKEIHSLSHIVSRKKHENISIGFVPTMGALHEGHISLTKQS
                     LLENEFTIASIFVNPLQFNNPDDLKKYPRTLDRDMQMLEAAGCNALFAPEASEVYIED
                     EQPLQFDLGNLANVMEGKFRPGHFAGVITIVHKLFSWVKPDVAYFGEKDFQQLAVIRK
                     MAKALHPDIIVTGCPTLREPNGLAMSSRNVRLSNTVRHDASIIYKAMQHAAQLKNILP
                     VYEVCSQVRSMIEEIAPFKVEYVEIADAVNLQSLQQWSDSQSPRIFVAVFADDVRLID
                     NAAL"
     gene            5694..6179
                     /locus_tag="PK134_00460"
     CDS             5694..6179
                     /locus_tag="PK134_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37356.1"
                     /translation="MSISNKLILWYERNKRELPFRSTNDAYRIWLSEVILQQTRVEQG
                     LPYYKRFLESFPTVKHLAKASETEVLKLWQGLGYYSRGRNLLATAQHITKIFHGKFPS
                     NYFELKKLKGVGDYTAAAIASMAFNESLLLMVMWLVYYQDCLQKKLRQIALKEKKCFR
                     N"
     gene            6098..6742
                     /locus_tag="PK134_00465"
     CDS             6098..6742
                     /locus_tag="PK134_00465"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRB37357.1"
                     /translation="MARVLSRLFAEKTTPNSTEGKKVFQELADSIGNKKFPGLHNQAM
                     MELGALVCTPRNPKCEICPLQIHCLAYQQKQQLNFPVKSKKIKIKERHFCFVIILYKD
                     KFYIRQRNGNDIWKNLYEFPLFETRTKFSSATISNFAKEYGIKKYESGRNHIHKLTHQ
                     HINASYIVAYLSKPLSSKKFPELKAVDAEDFKNNFPHPRLIEKILSENNYFNLL"
     gene            complement(6743..7621)
                     /locus_tag="PK134_00470"
     CDS             complement(6743..7621)
                     /locus_tag="PK134_00470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRB37358.1"
                     /translation="MAKASGKEKVKLSTKLRNKFRLVIMNDETLEEKASLLLSPINVV
                     VVVGIAIITLITATTYIIAFTPLREYIPGYTDVKVKRAAIESLIRTDSLQKQVSAQDL
                     YIQNLQNLITGQSQKTVSLDTVSVPQLYDTIKQLLHSEEDSLLRSQIESPQDYNLTLN
                     AGTTKGSIANFYFFAPLKGAITTRFNPTEKHFGVDVVAGPDAVIKSAMDGTVVIAEWT
                     SQAGYVIAIQHSNNMFSVYKHNSALLKKVGNVVKAGEVIAIVGNSGELSNGPHLHFEL
                     WYNGAPVDPTDYIAFE"
     gene            7745..10420
                     /locus_tag="PK134_00475"
     CDS             7745..10420
                     /locus_tag="PK134_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37359.1"
                     /translation="MKSSNHVKYFIILTLFLSACSTQKNTVINRAYHNLTAHYNGYFN
                     ARERVKEGTKTLSALHVDHYDRVLDVFKYATDDKAKTIYPDMDEAIKKSSIVIQRHSM
                     FINGKERCRWIPDNYMIIGKAQFLKHDFWTALETFQYVASTYKDNPKRFDGLIWQMQT
                     QMQLGKMADAEYMLDYLKNEKQLPSKYKPLFAAVAADFYLKKENYPKAIEQLTKAADL
                     ATKRADKIRYSYILAQIYQKVDSNDKAFAIYRKVIKMNPEYEMAFNAKINRARSFDVN
                     SADAQQVKDELNKMLKDEKNKDYLDQIYYALSGISMREKDTTEAFQFLKKSVAASTNN
                     ANQKGISYLEMGEISFRKLDFRAAQLYYDSAATNITSDYPDYALIQNKKTNLSRLIKN
                     LNIISHEDSLLTLGALSETEQYSRIDDIIAAEEAESLRKKQEEEAAKAKEQEEQSNMQ
                     FSQFQNQPGKDGMPGNSTGSSWYFYNQSAISFGFSEFAKKWGNRKLEDNWRRSSKESI
                     ATFGSEEEAEAQARADSLEQAQKALNDSIRQLNSEARRKAYFNNIPKTAQQKEESNAK
                     ILEAYYNAGIIYKEALGQPKPAADDMEEMMKRYPGNKYELPAYYNLYRCYLALFDEPK
                     AEYYKKIILNDYGDSEYARIITNPNYFKDAQRKTAIMQVFYENTYKAFLNRQYTDVIE
                     RKSAADSLFPGGTLAPKFAYLQALSIGYTQPRPQFEASLKKIVAAYPRDSVSILAKAI
                     LDKMNNVTPIDTTISESATTQTAEGNKMLNSIYEFMPDKPHNVLIIFSAELPYATTLK
                     GKINQFNQRNYKEKNLSVTELRVDDKNYMMVRSFPDHTQSKEYIQGLDSDQTVFEGMD
                     KSAFTLFPITEKNFNKFITDKDLSKYLDFYRVNYP"
     gene            10486..10917
                     /locus_tag="PK134_00480"
     CDS             10486..10917
                     /locus_tag="PK134_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB37360.1"
                     /translation="MFENLKNKTTTVKPGETASGSINLIAAGTSIEGEIKSNGDLRID
                     GHIVGSVFSKAKVVVGTTGVVDGDIVCQNADVSGTVKGTSTVTEMLFLKSTAKINGDI
                     NTGKLVVEVGASFTGNCNMGPMVKDIKNVERSEAKLQEKSA"
     gene            10874..11107
                     /locus_tag="PK134_00485"
     CDS             10874..11107
                     /locus_tag="PK134_00485"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRB37361.1"
                     /translation="MSKDQKPNSRKNQLNDYARYASMGFQMGAIMFIGTYGGYRLDKY
                     CQNKIPLFTVTFALIAVAAALYLTLKDFLHKKK"
     gene            11104..11478
                     /locus_tag="PK134_00490"
     CDS             11104..11478
                     /locus_tag="PK134_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37362.1"
                     /translation="MNKGFITYILQLAGITLLYILLYYLMKDFLAARFYPSWWKCGIF
                     FFVLFSVIHYLRLTKSKGNESIRFFMASTAFKLFLMLAIIVGYIFLNQTMAVPFVISF
                     CLAYFLFMIFDVAKAYTLMKTT"
     gene            11523..12612
                     /gene="prfB"
                     /locus_tag="PK134_00495"
     CDS             join(11523..11582,11584..12612)
                     /gene="prfB"
                     /locus_tag="PK134_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106623597.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRB37363.1"
                     /translation="MTQEQLTECKERVDALRGHLDIDVKLLKIKEEEQLTHAPDFWNN
                     PTRAEQILKSIKLKKNWTDTFAAVQQAVDDLGVLFDFSKTGDASEQEVEEQYTSVQKS
                     LEELEFKNMLSGEEDALSAVLTINAGAGGTESCDWASMLMRMYIRWAERMNYRINEID
                     RQDGDVAGIKSVTLEIEGDFAYGYLKSENGVHRLVRISPFDSNAKRHTSFASVFAYPL
                     IDDTIEIEIKDADIEMQTSRSGGAGGQNVNKVETKVQLKHLPTGIVVVCQVERSQSGN
                     RERAMQMLRSQLYELELRKRNEAKAATEAGKKKIEWGSQIRSYVFHPYKMVKDLRTDC
                     ETSNVQAVMDGELHEFMKAFLMEFGERV"
     gene            complement(12628..13032)
                     /locus_tag="PK134_00500"
     CDS             complement(12628..13032)
                     /locus_tag="PK134_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRB37364.1"
                     /translation="MKQILIVLLTIGLSFSSSAQQKTVLEANEKVAIISFEKIEHNFG
                     KIKEGDKAEHVFLFKNTGNTPLIVTDVSVTCGCTAAEKPDAPIMPGKDGKIKIVFNSA
                     GKVGSQNRTITVSTNAKNKITYLKMIGEVSEK"
     gene            13195..13509
                     /locus_tag="PK134_00505"
     CDS             13195..13509
                     /locus_tag="PK134_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRB37365.1"
                     /translation="MEEPKELYARVIETIKTCYDPEIPVDIYELGLIYEVKIIEGNDV
                     HVKMTLTSPACPVAESLPLEVEQKIKSIPDVNDAKVELTFDPPWDKDMMSEEARMELG
                     MW"
     gene            13512..14018
                     /locus_tag="PK134_00510"
     CDS             13512..14018
                     /locus_tag="PK134_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRB37366.1"
                     /translation="MEQEIKNRVADSGILQIDPKDFIPATTITELDIKGWLFEEMILK
                     EKDFREHIRQHNWQQYDGVAVAVYCSVDAIIPTWAFMLVSEALQPHTTNIFFGNATQL
                     KEKLTIDTIEKINIQNYNNKRIVIKGCGDENFSPAVYMLLTQKLKPVVKSIMYGEPCS
                     TVPVYKSK"
     gene            complement(14035..16191)
                     /locus_tag="PK134_00515"
     CDS             complement(14035..16191)
                     /locus_tag="PK134_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRB37367.1"
                     /translation="MTEFFNQLLHEIERPLTNPVLIFSLILFIILLSPILLRRLNIPG
                     IIGLIIAGVIIGPHGLYILEKNSAINLFSTIGLLYIMFIAGLELDLNEFKSHRNKSLV
                     FGFFTFIIPLGIGFPVCHYLLYYDFNASLLTASMFATHTLVAYPIVSKFGVSKNQAVA
                     ITVGGTILTDTAVLIILAVIMGNSQGNLNQEFWIRLGASLIIFSAIMFIVIPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFAAFLAEVAGVEPIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVSVIMNGPTALIIAGTLTIVAITGKWLASFLTQVIFK
                     YTSSQRQLIFGLSSAHAAATLAVILVGYKAGILDENILNGTIILILITCIIASFATER
                     AAKKIIVETEDDSSDLLKSSSASNEHILLPVANVGKMEKLLDFAILIKDKKSVNPVSV
                     LSVVSDNSEAEKNILKAKNKLEDFVKQASGTETKVNVITTIDHNAASGITRCSREIMA
                     DIIVLGWPQRTGFLDKLIGEKIESILNNTDKTTLICFLEKPLELHKRIVVAVPPLAEH
                     ENGFGLWLTKLAKLAQELSIPIHTYCNEATEKSIRKFFSKEKLAASLSVSLFSDWDDF
                     LVLARNIHADDFVVLVSARRGATSYMSILDGLPLKIEKHFSDNSRVIIYPQQYDGNIN
                     LENYSEVSTETLNRGIEVVQKIGKEIGSMLKKKNQE"
     gene            complement(16347..16826)
                     /locus_tag="PK134_00520"
     CDS             complement(16347..16826)
                     /locus_tag="PK134_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37368.1"
                     /translation="MRNIKSNIKNIFIATIIGFMLHGCSSKPSETVNSTKVSKAVNDE
                     PVNEPAEPIELDNGQKWKVNEEMKPFLTNGKNLLNEYIQNEDNDYTGLAAALSEQDNK
                     LIESCTMDGKSHEELHKWLHPHLDMVADLESTKDTAQAKKLVEQLKASYVDFEKYFQ"
     gene            complement(16908..18170)
                     /locus_tag="PK134_00525"
     CDS             complement(16908..18170)
                     /locus_tag="PK134_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB37369.1"
                     /translation="MQTIKYYLYIILISLLTGSCSTKNTTGEAENPVDAHAHESATTT
                     TLTSEQIKSIGVTTGGFEKKQLTASLKANGILKVPNQNRANATSLLGGTVKEILVQTG
                     ASVQKGQTIAIISNNQFISMQEEYLSISARAGLAEADFERQKTLKEGNAASLKAFQQA
                     EAELKSLRAKKSGLQKQLSLIGIDAQQLTPENIQSSVNIKSPINGSVSNVLVNIGSYL
                     EASNPVAEIVDNSQLHLDLYVYEKDLNKVKVGQTIHFTLTNNPGKEYDADIYAISNTF
                     EPNTKAVSVHATVKGDKHGLIDGMSITALISLENATYLAVPTEAIVSNEGQDFIFIVS
                     DKEVPPQEAAAHDHSAHDHAAHDHSAEPVTESGTVFERIPIRKGTTDVGYSEITLLKD
                     IPADSKVVTKGAFFILAKMTNQGEAHEH"
     gene            complement(18182..>21837)
                     /locus_tag="PK134_00530"
     CDS             complement(18182..>21837)
                     /locus_tag="PK134_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRB37370.1"
                     /translation="GLIGSFDDIKNIVVKQNPNGIPILIKDVAEVQFESAIRYGALTY
                     NGEKDVVGGIVMMLKGYNSAEVVNRVKEKMSTIQKSLPDDVVIEPFLDRTDLVKRAIS
                     TVEKNLIEGALIVILVLVLFLGNLRAGLIVASAIPLSLLFALGMMNVFGVSANLMSLG
                     AIDFGLIVDGAVIVVESSLYFLEHRKNMLRLTQNEMDDVVENRTKRMMSSAVFGQIII
                     MIVYLPILSLIGIEGKMFRPMAQTVSFAIIGALLLSLTYIPMMSALFLPKQLSHKKSF
                     NDKMMDFLQSKYTPLLNTAIRFKRAVVASAVVIFAIAVFVFSRMGGEFIPTLQEGDYA
                     FHCILPQGTSLSQSIETSMQCMREIKKFDEVKMVVGKTGSAEVPSDPMPTEATDMIIV
                     LKPKSEWKRKISYDELADEIMEKLEVIPGVFFEKSQPIQMRFNELMTGIRQDVAVKIF
                     GENMDTLLYYANRVNVVVQKVQGATEPSVERVAGLPQIVIRYNRSQIANYGLSIEDVN
                     HIVSTAFAGGKAGVVFENERRYDLMVRLDSASRNNLDDVGRLLIPVSNGMQIPLTQVA
                     DVKLELGPAQISRENGRRRIVVGFNVKDRDVASVVEEIESQLAEKVKLPEGYYYTYGG
                     TFENLQAASKRLMIALPIALALIFVMLFFTFKSAKQALLIYTTIPMSAIGGVFALLLR
                     DMSFSISAGVGFIALFGVAVLNGIVLIGTFNQLKQEGVEDILERIKEGTRSRLRPVLM
                     TATVASLGFLPMALSQSAGAEVQKPLATVVIGGLITATLLTLFVLPLFYLFFSSEKKV
                     KVEGNNFSGVMPIILLILSAMLLPTISSAQTNVTLEQAISLAMKNNLHLQAQQLNVQS
                     YESLKKSSFELPKTEVNFQYGQYNSIKKDNGINIQQTIPMPGYFVTKARLNNAELKGA
                     QLQQNIAIKQIISQVSSAYYEIVFLQQNEKELQKLDSIYSGFVQAATLRYTSGESNLL
                     EKTTAETKRGEVQLKVIRNNTALKIAYSTLEALLNTSNLIVTPISNFVPLVPDNVLDS
                     ATITNNFSYQLMLQQAAIAKRTKSAEAAMALPELNIGYMNQSIIGFQEVNGADVYFNR
                     SKRFTGFSMGITVPLTFFSNVSKVKSFSFRHQELLKEAENSKIELQSQLQNAFATYLQ
                     NLNQYNYFKNTAMPNADAIINTSTLAYRNGEINYLEYAAALENATAIRTGFLQSIHDV
                     NQTVININSLTNK"
BASE COUNT         6988 a         4112 c         3912 g         6815 t
ORIGIN      
        1 gtattgtaaa tagtgctcca catctctgac aggatcaata attaccgctt cttttccgct
       61 tgaaataata tagctgaatt gcgaatgacc ctgattataa aattgtttaa tttgcatagt
      121 aagttgaatg tggcaaatgt tttatttcga gacaacaact gaaatgactt ttatcactca
      181 tatcaagaat taatatcaac agctacattc ttcatcaatt gtaatattgt caggcaaatt
      241 aggttggagg ctatgagcaa tacagatact gtaaataagg tgaaatgcta tcattgtggt
      301 gatgactgtg atgaacaggt tatcacaagc gatgagaaaa ttttttgttg taccggttgt
      361 aagttggttt atgaaattct gcgtgaaaat aatttgtgta attattacaa cctgaacaac
      421 aatcctggca ctaaacagtc tgacattgtt caaaaacgat ttgccttctt agatgaacct
      481 tccatagaaa atcaactgat aagtttcaga gataaaaatg aaactatcat caccttttac
      541 attcctgcaa tgcattgtag cagttgtatc tggctacttg aaaattttca gaaagccgat
      601 gaaggtgttc tatcctcaag agttgatttt gttcgaaaag aattgcgttt ggtttatgat
      661 catcataaaa cttcgcttcg aaaaattgca gaaaaacttt cgcaaacagg ctatgagcct
      721 gcactgcaac ttgacgctat tgaaaagaaa aaaataaaaa aagcaaatag aagccgtatt
      781 ctcagaattg gtgttgccgg tttttgttct ggaaatatta tgatgctaag ttttcctgaa
      841 tatttttcat caggaaataa tattgatgct tcattacaaa cactattcag ctatctgatt
      901 ttgttgcttg cactacccgt atttttttac agtgcaaatg agttctttat taaatcttgg
      961 caggctctca aacaaaaaac cacttccata gatttgccga ttgccatagg tatatcagta
     1021 atttttttac gaagtgcatt tgaaataata acacaaacag gttcaggatt ttttgacagc
     1081 ggtagtggtc ttgttttctt catgctcatt ggtcgttggt ttcaggattt tacctttgat
     1141 agtttagctt tcgacagaga ttttaaatca tactttccca ttgcagttac ttctataaaa
     1201 aacggaaatg aaaaagaagt gcagattaat gacttgaaag ttaatgacag aattcttata
     1261 agaaataatg aaattattcc cgcagattgt attttactta aaggcaatgc ctcagtagat
     1321 tatcattttg taacaggtga gtcactacct gtttcacata cagccggtga atatatttat
     1381 gcaggaggca aacaagccgg tgccatgatt gaacttcagg tgatgaaaga agtttcgcag
     1441 agctatttag tacaattgtg gaatcgtagc tgggataaac aagatataag caagtttgaa
     1501 aaattagtga atgccatcag tcgttggttt atagtagtta cattttctat ttctacaatc
     1561 gctgctatct attggtggaa tacagatatt cataaagcta ttaatgcatt tacagcagtg
     1621 cttgtaattg catgtccctg tgcactggct ttatctgcac cttttactta tggaaacatc
     1681 ctccgcattc ttggcagaaa taaaatctac atgcgtaact atcgagtact cgaaaaaatt
     1741 gctgatgccg acactattgt atttgataag acaggaacat taactaagaa taatgtttct
     1801 gaaataagtt atcatggtca atcgcttgat gatgaggata aaaaaatact atcatcgcta
     1861 ttcaggcaat catcacatcc gttaagtaaa ttactgcaac aacatttgca agcagataaa
     1921 tattatccat gcaataattt tgaagaagtt accggtaaag gcatttgcgc agtattaaac
     1981 aataaaaaca ttaaagccgg aacatcagac tgggcaggag taaacaatga agttgaaaag
     2041 aatgcaacag cagtttttat caacattaat gacattaaaa aaggcaaatt tgttttcagt
     2101 aatatttatc gtgatgggtt gaacaccata tcacaaaaac tgaataacaa acataatttg
     2161 attgttctta caggtgataa tgaaagtgaa agaaaaaatc taactacact tttaggcaat
     2221 gccgaattac gatttaatca aaaacctgaa gacaaaagaa attacattct tgaattaatc
     2281 aatagtaaga aaaatgtgtt aatgattggt gatggcctga atgatgcagg tgcgctgcta
     2341 gcagccaata cgggaatttc agttaccgac aacactaata attttactcc tgcaagtgat
     2401 actattatgg atgctgcagc attacatcaa ctgcctgaat taattaaact atccaaatct
     2461 gctgtaacga taattatcat cagctttatt atttctttac tctataattt tgttggatta
     2521 gcctttgccg taagtggaac actttcgcca ttgtttgcag caatattaat gcctattagc
     2581 acaatcagtc ttgtactttt tacagttggt gcaagtacaa taaaagcacg gcaatcaggg
     2641 ttctaaagct caggattttt tccagttaaa gtgaacttca gtaccacatt ttactgttgg
     2701 tgaaaaccac accgttccac catattcttc aaccaccttt ttcacaatgc taaggcctac
     2761 acctgatgaa ggcactaccg atgatggaag cgtttgaaaa agttcgaaaa ttgttttttg
     2821 ttgttgtggc tttattccgg gtccgttatc tgctataaca aattcataag aatcattttt
     2881 ttctacaaca gaaatatcaa tagtattttt atcggaagga ttaaaagata ttgcattttg
     2941 aattaactct ttcaacacaa gttgcaatgc tgtcctgtct gtattcatat ctaaaaccga
     3001 agagttgaca ttcagtgaca cctttctatc accctgcagc tcagatgtaa tttgtctgat
     3061 cattttttca ggtgaaataa tatcgtgctg atgttttgta ttaccggaac gcgcataacg
     3121 caataagcca tcaagaagta atttcatctt agttactctg tttctaagca gccctatgtt
     3181 ttccttactc tcatcagaaa tctgcgattg taaatcttct tcaagccact gcacaagatt
     3241 ctcaatggca cgcagtggtg cattcaaatc atgcgaaagg atgtaaagaa acttatcaag
     3301 atgatcgttc agtcttttca gtttttcttc ctgttctctg tacttttgtt tatagcgaca
     3361 tccaaattca aagattgaaa ctattttctg tgtctcttca atatctaatg gcttcggaat
     3421 aaaatcaaac gatccgattt caaactcctc gagtgaaggg cgttcgcttt tggcaatggc
     3481 agaaacaaaa atgatgggta tatgctttgt tttaggattg gcacgaagta attgtgctac
     3541 ctcaacgcca tccatgtcag gcatttgaat gtctaataat atcaaatcaa ttggttcttc
     3601 catcagtaat tttaaagctt cattacccga aaaagctttt ataagaattc ggctatcatc
     3661 ttcaagaata ccttcaagtg tcaataaatt agccggaata tcatcaacta atagaattct
     3721 taatggtgtt ttgtctgtgg cttgtatgct acgacccgac agctctgatg tgttgattat
     3781 cattttttag acttaatatn nnnnnnnnnc agcccgatta aaaaggttgc tattacaaca
     3841 cttttaggta tttaaatgaa cttctgcatg gaattactta caccgcaaaa ttttaaagat
     3901 tatgaactgc ttgatagcgg aaactttgaa aagttagagc gttttggtca gtacattacc
     3961 atcaggcccg aaccacaagc tgtgtggaac aggcaactaa aagaggccga ttggcttaaa
     4021 aaagcacatg taaagtttat agctaaaaac agctcgtcag gtacatggaa aaaccttaaa
     4081 gagattcctg accgttggca tattgcctat gcattgcctg atgaaaaaca aattcgactt
     4141 aggctggcat taacatcatt taagcatgta ggtgtttttc cggaacaggc cgtaaactgg
     4201 gattttattt ataattcagt taaaaaatta gatgtagtac aaccaaaagt cctcaacctc
     4261 tttgcctaca ccggtgcatc aacacttgct gccaaagctg ccggtgccga catcattcat
     4321 ttagactcta taaaacaggt tgtaacatgg gcaaaagaaa atatgacatt gagtggcttg
     4381 gacaacattc gctggttggt tgaagatgcc atgacctttg tgaaacgaga agtaaaacgc
     4441 gggaaaaaat atcagggaat tattttagat ccacctgctt atggccatgg accaaatggc
     4501 gaaaactgga aattagaaga tgagataaac gaaatgcttg ccaacgttaa gttgctgctt
     4561 gaagaaaatc actttcttgt actcaacacc tattcacttg gtttttcatc tataatttta
     4621 gataatctgc ttgacgaaca ttttagtaaa aatattaaac gacagacagg agaaattttt
     4681 ctacaaagca ctgccggagc tagacttcca ttaggtgtct ttgcacggca ttgtacggtg
     4741 tagttattta caatgctgca ttatcaatca gccttacatc atctgcaaaa actgcaacaa
     4801 aaatacgtgg tgactgtgag tctgaccatt gttgtaaaga ctgtaggttt actgcatcag
     4861 caatttcaac atactcaact ttaaaaggag ctatttcttc aatcatactt ctcacctgcg
     4921 aacaaacttc gtaaacgggc agtatgtttt tcagttgcgc agcatgttgc attgccttat
     4981 aaattatgga tgcatcatga cgaacggtat tacttaatct tacattgcgc gaactcatgg
     5041 caagaccatt aggctcacga agtgtagggc agcctgtgac aataatatca ggatgtaatg
     5101 ctttagccat ttttctgata acggcaagct gctgaaaatc tttctctccg aaataagcaa
     5161 catcaggttt aacccatgaa aaaagtttgt gaacaatagt gataactccg gcaaaatgtc
     5221 ccggacgaaa tttgccttcc ataacgtttg caagattgcc taaatcaaac tgcaatggtt
     5281 gttcatcttc aatataaact tctgatgctt ccggtgcaaa caaggcatta caacctgccg
     5341 cttcaagcat ctgcatatct ctatctaagg tacgaggata cttttttaaa tcatcaggat
     5401 tattaaactg taatggattt acaaaaatgc tggcaatagt aaattcattt tcaagcagcg
     5461 actgtttagt taatgaaata tgtccttcgt gtaatgcgcc cattgttggc acaaaaccaa
     5521 tgctgatatt ctcgtgcttc tttctgctaa caatatgaga aagagagtgt atttctttaa
     5581 ttaatttcat aacaaaaagc gatggcaatg ataagtaaat ttagttgtta ctgaattgtc
     5641 tattaaattt aaactgtaat tattttttat aatgactttc tttgccaaag caaatgagta
     5701 tttcaaacaa acttatttta tggtatgaac gcaacaagcg cgagttgcct tttcgcagta
     5761 caaacgatgc ctatagaata tggttgagcg aagttatcct tcagcagaca agagtagaac
     5821 agggattgcc ttattacaaa cggtttttag aatcgttccc tacagtgaag catcttgcca
     5881 aagcatcaga aaccgaggtg ctcaaattgt ggcagggatt aggatattat tcaagagggc
     5941 gaaatctttt agccacagca caacatatta ccaaaatatt tcacggtaaa tttccttcca
     6001 attattttga gcttaaaaaa ctaaaaggtg taggcgatta tactgcagca gctattgctt
     6061 caatggcatt taatgaatct ttgctgttga tggtaatgtg gctcgtgtat tatcaagatt
     6121 gtttgcagaa aaaactacgc caaatagcac tgaaggaaaa aaagtgtttc aggaattagc
     6181 cgatagtata ggcaataaaa aatttcccgg cctgcacaat caggcaatga tggagcttgg
     6241 tgcattggtt tgtacaccac gaaatcctaa gtgtgaaata tgtcctttac aaattcattg
     6301 tttggcatat caacaaaagc aacaacttaa ttttccggta aaaagtaaaa agattaaaat
     6361 caaagagcgt cacttttgtt ttgtcataat actttacaaa gacaaatttt atatcagaca
     6421 acgtaatggt aacgacatct ggaaaaacct ctatgagttt cctttgttcg aaaccagaac
     6481 aaaattttca tcagccacta ttagcaattt tgcaaaagaa tatggtatta aaaaatatga
     6541 atcaggaaga aatcatattc ataagctgac acatcaacac attaatgcat catacattgt
     6601 ggcatattta agcaagccac tatcatcaaa aaaatttcct gagttaaaag cagttgatgc
     6661 cgaagatttc aaaaacaatt tcccacatcc aaggttaatc gaaaaaatac tttctgaaaa
     6721 caactacttt aatctgctgt aattattcaa aggctatata atcagtaggg tcaacaggcg
     6781 caccattgta ccaaagttca aaatgcagat gcggaccatt acttaattcg cccgagttac
     6841 ctacaatagc aataacttct ccggctttca ccacattacc tactttttta agcaatgcag
     6901 aattatgttt ataaacagaa aacatattgt tactgtgttg aatggctatg acataacctg
     6961 cctgcgaagt ccattctgca atgaccaccg ttccgtccat ggcactttta ataacagcat
     7021 cagggccggc aactacatca acaccaaagt gcttttctgt aggattaaac cgtgttgtta
     7081 ttgctccttt taatggcgca aaaaagtaaa agttagcaat agaaccttta gtggtccctg
     7141 cgtttaatgt taggttgtaa tcttgtggcg actctatctg acttctcagc aaagaatcct
     7201 cttcggaatg aagcaattgt tttattgtat cataaagctg tggcacagaa actgtatcga
     7261 gcgaaacagt tttctgtgac tgaccggtaa taagattttg cagattttgt atgtacaaat
     7321 cctgtgcaga cacctgtttt tgtaaagaat cggtacggat aaggctttca atggctgccc
     7381 gttttacttt aacgtctgta tatccgggaa tatattcacg cagtggtgta aaggcaataa
     7441 tgtaggttgt ggctgtgatg agggtgatga tggcaatacc caccaccacc accacattga
     7501 ttggtgagag cagcaacgaa gccttttctt ccagagtttc gtcattcata atgactaacc
     7561 ggaacttatt acggagtttg gtgcttagtt tgactttttc tttgcctgat gccttggcca
     7621 tgcgttgttt aaacaaaaaa gtattgttat gtttgccttc ttaatcgggc aaatatcgta
     7681 ataattaaac gagcaataat tttcgctgga aaaagttaat atttatcgca acaaccatat
     7741 tgttttgaaa tcatccaatc acgtaaaata ttttatcatc cttacgctgt ttctatcagc
     7801 atgttcaaca caaaagaaca ctgtcataaa cagggcatac cataacctta ctgcacacta
     7861 caacggctat tttaatgccc gcgaaagagt gaaagaaggt acaaaaactt tgtctgcatt
     7921 gcatgttgat cattacgacc gtgtgcttga tgtttttaaa tatgcaactg acgataaagc
     7981 caagaccatt taccctgaca tggatgaggc cattaagaaa agctcaatag ttattcaacg
     8041 acactccatg tttattaatg gtaaagagcg ttgccgctgg ataccggaca actacatgat
     8101 aatcggtaaa gcgcaatttc taaagcatga tttctggact gcattagaga cttttcaata
     8161 cgttgcatct acctataaag ataacccaaa aagatttgat ggcttaatct ggcagatgca
     8221 aacacaaatg cagttaggca aaatggccga tgcagaatac atgctcgact atttaaaaaa
     8281 tgaaaagcaa ttgccatcaa agtacaaacc tctttttgct gctgtggctg cagattttta
     8341 tctgaagaaa gaaaattatc ctaaagctat tgagcaatta accaaagctg ctgatctggc
     8401 gacaaagaga gctgacaaaa tccgatatag ctatatcctt gcacagatat atcagaaggt
     8461 tgattccaac gacaaagctt ttgcaatcta ccgcaaagta atcaagatga atcccgagta
     8521 tgagatggca tttaatgcaa aaatcaacag agcgcgttca tttgatgtaa acagtgccga
     8581 tgcacaacaa gtaaaagatg agttgaacaa gatgcttaaa gatgaaaaaa acaaggatta
     8641 tcttgatcag atttattatg cactgagtgg aatttctatg cgtgaaaaag atacaacaga
     8701 ggcatttcaa tttttaaaga agtctgtggc agcaagcacc aacaacgcca atcaaaaagg
     8761 tatttcatat ctggagatgg gtgaaataag tttccgcaaa cttgacttcc gtgctgcgca
     8821 attatattat gacagtgctg ccaccaatat cacttctgac tatcctgatt atgcattgat
     8881 tcagaataaa aaaaccaatc tgtcgcgatt gataaaaaac ctgaacatca tttcacacga
     8941 ggacagtctg ctcacattgg gagcactttc agaaacagaa caatattcgc gcattgacga
     9001 cattattgct gcagaagaag cagagtcgct gcgtaagaaa caggaagagg aggcagccaa
     9061 agctaaagag caggaagagc agtctaacat gcagttcagt cagtttcaaa atcaaccagg
     9121 aaaagatggt atgcctggaa attcaacagg tagctcatgg tatttttata atcagagtgc
     9181 cattagtttt ggcttttcgg agtttgccaa gaaatggggc aacagaaaac ttgaagataa
     9241 ctggcgtagg agcagcaaag aatccattgc aacttttggc agtgaagaag aagccgaagc
     9301 gcaagcacgt gccgacagtt tagagcaggc acaaaaagca ttaaacgaca gcatcagaca
     9361 attgaatagc gaagcacgca gaaaagcata ctttaacaac attccaaaaa ctgctcagca
     9421 aaaagaagaa agtaatgcta aaatattaga agcatattac aatgcaggaa tcatttataa
     9481 agaagcatta ggtcagccaa agcctgctgc tgacgatatg gaagaaatga tgaaacgata
     9541 tccgggtaat aaatatgaac tacctgctta ttacaatctg tatcgttgtt acttagcttt
     9601 atttgatgaa cctaaagccg agtattataa aaaaattatt ctcaatgact atggcgattc
     9661 agaatatgca cgcatcataa ccaacccaaa ttattttaaa gatgcacagc gcaagacagc
     9721 catcatgcag gtgttttatg aaaatacata caaggctttt ctcaatcgtc aatatacaga
     9781 tgtcatagag cgtaaatcgg cagcagactc actatttccc ggtggcacat tagcgccaaa
     9841 gtttgcctac cttcaggcac tttctatcgg atatacacag ccccgcccac agttcgaagc
     9901 ttcactgaaa aaaattgttg ctgcataccc aagagattct gtcagcatat tggcaaaagc
     9961 tattcttgat aaaatgaata acgtaacgcc aatagataca acaatcagcg aatcggcaac
    10021 aacacaaact gccgagggca ataaaatgct gaattctatc tatgagttta tgccggacaa
    10081 accacataat gtgctgatta ttttctctgc tgaactgcct tatgccacaa cattaaaagg
    10141 aaaaatcaat cagtttaacc agcgcaacta taaagagaaa aatctgagtg tgacagagtt
    10201 aagagttgat gacaaaaact atatgatggt gcgttcattt cccgaccaca cgcaaagcaa
    10261 ggaatatatt caggggttgg actctgatca aacggttttt gaaggcatgg ataaaagtgc
    10321 ttttaccctt ttccccatta cagaaaaaaa tttcaacaag ttcattaccg ataaggattt
    10381 gagcaaatac cttgatttct accgtgttaa ttatccataa tttttggttt tgttaataac
    10441 ttttattttt atctttgccg caatacataa ttaaaagttt ttaccatgtt tgaaaatttg
    10501 aagaacaaaa ccaccaccgt taaacccggt gaaactgcat caggctcaat taacttaatt
    10561 gctgccggaa cttcaataga aggtgaaatc aagtcgaatg gcgacttgcg aattgacgga
    10621 cacattgtag gctcagtatt ttcaaaagct aaagtggttg ttggcacaac cggagtagtg
    10681 gatggagaca ttgtttgcca gaatgcagat gtttcaggaa cagtaaaagg aacatctacc
    10741 gttactgaaa tgttgtttct aaagtcaact gcaaaaatca atggcgacat taacaccggc
    10801 aaattagttg ttgaagttgg ggcaagtttt acaggcaact gtaatatggg gcctatggtt
    10861 aaagacataa aaaatgtcga aagatcagaa gccaaactcc aggaaaaatc agcttaacga
    10921 ttatgcccgg tatgcgtcca tgggatttca aatgggcgct atcatgttta ttggtaccta
    10981 tggaggttac cgcttagaca aatattgtca aaacaaaatt ccactattca ctgttacttt
    11041 tgccttgatt gctgttgcag ctgcacttta cttaacattg aaagattttt tacataagaa
    11101 aaaatgaaca aaggtttcat cacctatatt ttacaacttg ccggcataac cctgctgtat
    11161 attttgcttt attatttgat gaaagatttt ctggctgcac gtttctatcc ttcatggtgg
    11221 aaatgcggaa tatttttctt cgtacttttt tctgtcattc attatttgcg acttaccaag
    11281 agtaaaggta atgagtccat ccgttttttt atggcatcaa cagcgtttaa attatttctg
    11341 atgcttgcta tcattgtagg ctacattttt ctgaatcaaa caatggcagt tccttttgta
    11401 attagttttt gcttagccta ttttttgttt atgatttttg atgtagcaaa agcctatacg
    11461 ctgatgaaga caacatagtc tgaaaatgcc ggaaaacatt aattcgtaaa ttcgcagccc
    11521 gtatgacaca agaacaatta acagaatgta aggagcgcgt ggacgctttg agggggcacc
    11581 tttgacattg atgtcaaact gcttaaaata aaagaagaag aacaacttac acacgcacct
    11641 gatttctgga acaaccctac aagggcagaa cagattttaa aatccatcaa gcttaaaaaa
    11701 aactggacag acacgtttgc tgccgtacag caagctgttg atgatttggg tgtattattc
    11761 gatttttcaa aaacaggcga tgcttcagaa caagaagtag aagagcaata tacctccgtt
    11821 caaaaatcat tagaagagct tgaatttaaa aacatgctca gtggtgaaga agatgcattg
    11881 agtgcagtgc tcacaataaa tgccggtgcc ggaggaacag aaagttgcga ctgggcatca
    11941 atgctcatgc gtatgtatat ccgctgggcc gaacggatga actatcgcat aaacgaaatt
    12001 gacagacaag atggcgatgt tgcaggaata aaatctgtta ctttagaaat agaaggtgat
    12061 tttgcatacg gatatttaaa gagcgaaaat ggtgtacatc gtttagtacg catttctcca
    12121 tttgattcca atgcaaaacg tcatacatca tttgccagtg tgtttgccta tcctttgatt
    12181 gacgatacta tagagattga aattaaagat gcagatatag aaatgcagac ttcccgttca
    12241 ggtggtgcag gtggacaaaa tgtaaataaa gtagaaacaa aggttcaact caaacacttg
    12301 cccacaggta ttgttgttgt atgtcaggta gaacgttcgc aaagtggcaa ccgcgagcgt
    12361 gccatgcaga tgttgcgttc gcaactttat gaacttgaac ttcgcaaacg caatgaggct
    12421 aaggcagcga cagaagcagg taagaaaaaa attgaatggg gctcacaaat aagaagctat
    12481 gtttttcatc catataagat ggttaaagac ttacgcacag attgtgaaac atcaaacgtt
    12541 caagccgtaa tggatggcga actacatgag tttatgaaag cctttctgat ggaatttgga
    12601 gaaagagttt aaccattttc tcagaaatta tttttcactc acttcaccta tcattttcaa
    12661 ataagtaatt ttattttttg cattggtact taccgttatt gtgcggtttt gtgaaccaac
    12721 tttaccggca gagttaaata ctatctttat ttttccgtct ttaccgggca taattggcgc
    12781 atcaggtttt tctgctgccg tacatccgca ggtaacactc acgtcagtaa caattaacgg
    12841 tgtgttgcct gtatttttaa aaagaaaaac atgttcggcc ttatcacctt ctttaatctt
    12901 accgaaatta tgttctattt tttcgaaact gattatagca actttttcat tggcctccaa
    12961 aacagttttt tgctgcgctg atgatgaaaa tgatagacca attgtaagga gaacgattaa
    13021 aatttgtttc ataatattta tttttaaaaa acttttactt gactttatag ttttcaaaga
    13081 tgggattaat attgatgctt tgcatgaatt tattttgaat cttctattga ttgtaaaatc
    13141 agaataaaac agcaataaaa tcaagacaac aataaaacca ttaaatttgc acttatggaa
    13201 gagccaaaag aattatatgc cagggttata gaaaccataa agacttgtta cgatccggaa
    13261 attccggttg acatttatga gttaggattg atttacgaag taaagattat tgaaggaaat
    13321 gatgttcatg taaagatgac tctgacttca cctgcttgtc cggttgcaga atcgctgcca
    13381 ttggaagttg aacaaaaaat aaaaagtatt cctgatgtga atgatgcaaa agttgaatta
    13441 acttttgatc caccatggga taaagatatg atgagtgaag aggctcgcat ggaacttgga
    13501 atgtggtaat tatggaacag gaaattaaaa atcgcgttgc cgacagtggt attctacaga
    13561 tagatccaaa agattttata cctgccacaa caataaccga acttgacatt aaaggatggt
    13621 tgtttgagga aatgattctt aaagaaaaag atttcagaga gcatatcaga caacataact
    13681 ggcagcagta tgatggcgtt gcagttgctg tttattgttc tgttgatgcc attattccga
    13741 cttgggcatt tatgctcgtt agtgaagcat tgcagccaca taccaccaac attttttttg
    13801 gtaacgctac tcaactcaaa gaaaagctaa ccattgatac aattgaaaaa atcaacattc
    13861 aaaactataa taacaaacga atagtaatta aagggtgtgg tgatgaaaac ttttcgcctg
    13921 ctgtttatat gctgttgaca caaaaactaa aacctgttgt taagagtatt atgtatggcg
    13981 aaccatgttc tactgttcct gtttataaat caaaatgatt aatcaagaat aaatctactc
    14041 ttgatttttc ttttttagca ttgagccaat ttctttaccg attttctgaa ccacctcaat
    14101 acctctgttt aaagtttctg ttgaaacctc gctatagttt tccagattaa tattgccgtc
    14161 atattgttgc ggataaataa taactcggct gttgtcgcta aaatgttttt ctattttcag
    14221 cggaagtcca tctaaaatac tcatgtaaga agttgcacca cgtctggcag aaaccaaaac
    14281 aacaaaatca tcagcatgaa tatttcttgc cagtaccaga aaatcatccc agtcgctaaa
    14341 caaactaacg ctcagtgatg ctgctaattt ttctttactg aaaaattttc ttattgattt
    14401 ttcagttgct tcattacaat atgtatgaat tggaatactg agttcctgtg ccagctttgc
    14461 caatttagta agccataaac caaatccatt ttcatgttcg gcaagaggtg gtacagcaac
    14521 cactattcgc ttatgaagtt caagtggttt ttcaagaaag caaattaaag ttgtcttatc
    14581 tgtattgttt aatatacttt caattttttc accaataagt ttatcaagaa atcctgtacg
    14641 ctgtggccat cccaaaacaa tgatgtcggc cataatttct cttgagcaac gggtaattcc
    14701 gcttgctgca ttatgatcaa ttgtagtaat tacgttaact ttagtttctg ttcctgaggc
    14761 ttgtttaaca aaatcttcta atttattttt tgcctttaaa atatttttct ctgcctcgct
    14821 attgtcagat acaacagaaa gaacagacac tgggttaaca gattttttat cctttattaa
    14881 aattgcaaag tcaagcagct tttccatttt accaacattg gctacaggaa gtaaaatatg
    14941 ttcattggaa gcactgctac tttttaaaag atcgctgctg tcatcttctg tttctacaat
    15001 tattttttta gcggcacgct ctgttgcaaa agaagcaatg atacatgtaa ttaaaataag
    15061 aataatggtg ccgttgagga tattctcatc aagaatgcct gctttataac ctactaaaat
    15121 aacggcaagt gttgcagcag catgagcact gcttaaaccg aaaataagtt gccgttgcga
    15181 tgatgtatat ttaaaaataa cttgtgtcag aaaagatgca agccattttc ctgttattgc
    15241 aacaattgtt aatgtacctg ctatgatgag tgcagtaggt ccattcatga taacactaac
    15301 atcaaccaac atccctacac tgatcaggaa aaatggaata aacaaagagt ttccaataaa
    15361 ctcaattctg ttcatcaatg ctgatgaatg tggaatcagt ttgttcaatg ccaaacctgc
    15421 aacaaaggct cctatgattg gttctactcc ggcaacctct gcaagaaagg ctgcaaaaaa
    15481 caccacagag agtacaaaaa tataatgcga atgtttttca ctttcgagtt ttctgaaaaa
    15541 ccattttgca attctcggaa taacaataaa cattattgca gaaaaaataa tcagtgatgc
    15601 ccctaaacga atccagaatt cctgattcag gtttccctgg ctgttgccca tgattactgc
    15661 aaggataatc agtacggcag tgtctgttaa aatagttcct cctaccgtaa tggcaacggc
    15721 ctgatttttt gaaacaccaa acttgcttac aatgggatag gcaacaagcg tatgtgtagc
    15781 aaacatgctt gctgtcagaa gacttgcatt aaagtcataa tacaacaagt aatgacaaac
    15841 cggaaagcca atacctaaag gaatgatgaa agtaaaaaaa ccaaagacca aacttttatt
    15901 gcgatgagat ttaaattcat tcagatcgag ttcaagtccg gcaataaaca tgatgtaaag
    15961 caaaccaatg gttgaaaaca gattgattgc tgagtttttt tcgagaatat ataaaccatg
    16021 tggcccgata attactcctg caatgatgag cccaatgatt ccgggaatat tcaaacgcct
    16081 cagcaaaatg ggagaaagca gaatgataaa taatatcaaa gaaaaaatca gtaccggatt
    16141 ggtaagtggt cgttcgatct catgaagtag ttgattaaaa aattctgtca tttatgctat
    16201 cgtcctatca tttttgagtt aagtggttta tttaatctga ctaacgaatg tcatatatag
    16261 cagtgttttg cctctcatta ttatcacact cagtagaacc tacttctgcc tgcagtttac
    16321 taagtaaaca aaaaatattt cagtacttat tgaaagtact tttcaaaatc aacataagag
    16381 gccttcagtt gttccaccaa ctttttagct tgtgctgtat cttttgtact ctcaagatcg
    16441 gcaaccatat ctaaatgtgg atgcagccat ttatgtaatt cttcgtggct tttaccatcc
    16501 attgtacagc tttcaatcaa cttattatcc tgctcactaa gtgctgctgc taaacctgta
    16561 tagtcgttat cttcgttttg aatgtattca ttcaacaagt ttttgccatt tgtaagaaat
    16621 ggtttcatct cctcatttac tttccacttt tggccattat ccagctcaat aggctctgca
    16681 ggttcgttta caggctcatc attaactgct ttactcacct tagtgctgtt tactgtttcg
    16741 cttggtttac tactacatcc gtgcagcata aatccgataa tagttgcaat aaaaatattt
    16801 ttgatgttgc tttttatatt tctcataata tattctttca gttgtcaaat ttaatcaatc
    16861 aaatccaaat aagttactca gatgttactt tattaagttt ttaataatca atgctcatgt
    16921 gcctcaccct gattggtcat cttagccaga ataaaaaatg cacctttagt aaccacctta
    16981 ctatcggcag gaatatcttt taataaagta atctccgaat aacccacatc tgttgttcct
    17041 tttctgatag gaattctttc aaacactgta ccggactctg taacgggttc agcactatgg
    17101 tcatgtgctg catgatcatg tgcactatgg tcatgtgcag cggcctcttg tggtggaact
    17161 tctttatcag agacaataaa aataaaatcc tggccttcat ttgaaacgat tgcttctgtc
    17221 ggtactgcca aataagttgc gttttctaag cttatcaaag cagtaatgct catgccatca
    17281 atcagtccat gtttgtcacc tttaacagtt gcatgtacag agactgcttt tgtgtttggc
    17341 tcaaacgtat tactgatagc ataaatatca gcatcgtatt ctttacccgg attattggtg
    17401 agtgtgaaat gtattgtctg tcctacttta accttgttga ggtctttttc atacacatac
    17461 aagtcaagat gcaattgact gttgtcaaca atctctgcaa caggattact tgcttcaaga
    17521 taacttccga tatttaccag tacattgctt acagatccat taatggggct tttaatattt
    17581 actgacgatt gaatattttc aggagtcaac tgctgggcat caataccgat taaagacaat
    17641 tgcttttgta agcctgattt ttttgcacgt aatgacttca gttctgcctc tgcctgctga
    17701 aaggctttca acgatgctgc attaccttct ttcaacgttt tttgtctttc aaaatctgct
    17761 tctgcaagac ctgcacgtgc acttatactg agatattctt cctgcatact gatgaactga
    17821 ttgttactga taatagcaat ggtttgccct ttctgtaccg atgctcctgt ctgaactaat
    17881 atctctttca ctgtaccgcc aagtaatgaa gttgcatttg cacgattttg attcggaact
    17941 tttaaaattc cgtttgcctt cagtgaagct gtcaattgtt tcttttcaaa ccctcctgtt
    18001 gtgacaccaa tagattttat ttgctctgaa gtgagtgtgg ttgtagttgc actttcatgt
    18061 gcatgtgcat caacagggtt ttcggcttca ccggttgtgt tttttgtgct gcatgaacct
    18121 gtcagcagcg aaattaatat gatatataaa taatacttta tcgtttgcat tgttctgatt
    18181 tttatttatt ggttaaagaa tttatgttga tgactgtttg atttacatcg tgaatacttt
    18241 gcagaaagcc tgttcgtatt gctgttgcat tttcaagtgc tgctgcatat tcaagataat
    18301 ttatttcacc gtttctgtat gctaatgttg atgtgttgat tattgcatcg gcatttggca
    18361 ttgcagtgtt tttaaaataa ttgtactgat tcaggttttg cagatatgtg gcaaatgcat
    18421 tctgtagctg actttgcaac tctattttac tgttctccgc ctctttaagc aattcctgat
    18481 gtctgaatga aaatgatttt actttactga cattactaaa gaatgtcagt ggtacagtga
    18541 tgcccatgct aaaccctgtg aatcgcttgc ttctattaaa ataaacatct gctccattca
    18601 cttcctgaaa gccaatgata ctttgattca tataaccaat attcaattca ggtagtgcca
    18661 tggctgcttc tgcagacttt gttcttttag caattgctgc ttgttgtaac atgagttgat
    18721 aagaaaaatt gtttgtaatc gtagctgaat ccaaaacatt atcgggtaca agaggcacga
    18781 aattacttat cggtgtcaca ataagatttg aagtatttaa aagtgcttct aatgttgagt
    18841 aagcgatttt aagtgctgta ttatttctaa taacttttaa ttgcacttca ccacgtttgg
    18901 tttctgccgt tgttttttca agcaggttac tttcaccgga agtgtaacgc aatgttgctg
    18961 cctgtacaaa gcccgaataa atgctgtcca atttttgtag ctctttttca ttctgctgta
    19021 aaaagacaat ctcataataa gcagacgaaa cctgagagat aatttgtttg attgctatat
    19081 tctgctgtag ttgtgctcct tttaactcag cattgttcaa tcgtgctttt gtaacaaaat
    19141 atccgggcat cggaatggtt tgttgtatat ttattccatt atcctttttt atactgttgt
    19201 attgtccgta ttgaaaattt acttctgtct ttggcaactc aaaacttgac ttctttaaag
    19261 actcatacga ttgtacattt aattgttgtg cctgaagatg taaattgttt ttcattgcta
    19321 atgaaatggc ttgttccaat gtaacatttg tttgtgctga tgatattgta ggaagcaaca
    19381 tagctgataa tatcaaaaga attataggca ttactcctga aaaattattt ccttcaactt
    19441 taactttctt ctctgaagaa aaaaacaaat aaaataaggg tagcacaaaa agtgtaagca
    19501 gtgttgcagt gataagaccg ccaataacta ctgttgccaa aggcttctgc acttctgctc
    19561 ctgcactttg cgacaatgcc attggcaaaa atcctaacga tgccacagta gcagtcatca
    19621 atacaggacg aagtcgtgac cttgttcctt cttttattcg ctccagaata tcttcaacac
    19681 cttcctgctt taactgatta aaagtgccga tgagaacaat tccatttagc acagctacac
    19741 caaacaaggc aataaagcca acaccggcag aaatactaaa agacatatcg cgcaacaaca
    19801 atgcaaaaac accaccaatg gcactcatgg gtatggttgt ataaatcaac aatgcctgct
    19861 tggccgactt aaacgtaaaa aacaacataa caaaaatgag tgctaaggca ataggcaaag
    19921 caatcatcaa tcttttactt gctgcctgaa gattttcaaa tgttccgcca taggtataat
    19981 aatatccttc gggtaatttt actttttctg caagctgcga ttctatctct tcaacaacac
    20041 ttgccacatc gcggtctttt acattaaagc caacaactat tcttcttcgt ccgttttcgc
    20101 gactgatttg tgcagggccc aattccaatt ttacatcagc aacctgagtt aatggaattt
    20161 gcataccatt actgactggt ataagtaacc tgccaacatc atccagatta tttctgcttg
    20221 cactgtctaa acgcaccatc aggtcatagc gtctttcgtt ttcaaaaacc actccggctt
    20281 taccaccagc aaatgccgtg ctcacaatgt ggtttacatc ttctattgac aatccatagt
    20341 ttgctatctg cgaacgattg tatctgatga ctatctgagg caatcctgca acacgttcaa
    20401 cacttggctc cgttgctccc tgtacttttt gtacaactac atttactcga ttggcataat
    20461 aaagtaatgt gtccatattt tctccgaaaa tttttactgc aacatcctgt cttatccctg
    20521 tcatcaattc gttaaaccgc atttgtatag gttggctttt ttcaaaaaaa actccgggaa
    20581 taacttccaa cttctccata atttcatctg caagctcatc ataactgatt tttcttttcc
    20641 actcactttt gggtttaagt acaataatca tatccgtagc ttcagtaggc attggatctg
    20701 aaggcacctc tgcactaccc gtcttgccta caaccatttt cacttcatca aactttttga
    20761 tttcacgcat acattgcatt gaagtttcta tgctttgtga taacgatgtg ccctgtggta
    20821 atatgcaatg aaaagcataa tcgccttcct gaagtgttgg aataaactca ccacccattc
    20881 ttgaaaagac aaaaacagca atagcaaata tcacaacagc gcttgcaacc acagctcttt
    20941 taaaacgaat agcagtgtta agcaacgggg tatatttact ttgtaaaaaa tccatcattt
    21001 tgtcgttgaa agattttttg tgcgaaagtt gtttgggtag aaataatgca ctcatcattg
    21061 gaatgtaggt gagtgaaagt agcagcgccc caataatggc aaatgaaact gtctgcgcca
    21121 ttggcctgaa catttttcct tcaatgccta ttaaactaag tatgggcagg taaacaatca
    21181 taataattat ctgaccaaaa actgcactgc tcatcattct tttggtgcgg ttttccacca
    21241 catcatccat ttcattttgt gtaagtcgta gcatattttt tctatgctcc agaaaataaa
    21301 gcgaagattc aacaacaatt actgcaccgt caacaataag tccgaagtca attgctccaa
    21361 ggctcatcag attcgcacta acaccaaaca cattcatcat tccaagagca aacaacagtg
    21421 acaaaggaat agcgctggct acaattaaac ccgctcttag atttccaaga aacaaaacca
    21481 acacaagaat aactattaat gccccttcaa tcaggttctt ttctactgta gaaattgctc
    21541 tttttaccaa atcggtgcga tcaagaaatg gttcaataac tacatcatca ggcaatgatt
    21601 tctgtatagt actcattttc tcttttacac gattcactac ttctgcactg ttgtagcctt
    21661 ttagcatcat cactatgcca ccaacaacat ctttctctcc attatatgtc aatgcaccat
    21721 agcgaattgc actttcaaac tgaacttcgg ctacatcttt aatcagaatt ggaataccat
    21781 ttggattttg tttgactaca atattcttta tatcatcaaa cgaacctatt aaaccaa
//
LOCUS       DAORQT010000006        21046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_168_length_21046_cov_15.283217, whole genome shotgun sequence.
ACCESSION   DAORQT010000006 DAORQT010000000
VERSION     DAORQT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_21046_cov_15.283217"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="ligA"
                     /locus_tag="PK134_00535"
     CDS             <1..1099
                     /gene="ligA"
                     /locus_tag="PK134_00535"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRB37371.1"
                     /translation="SPKWAIAYKYKAESASTILNSVVYQVGRTGTITPVANLKPVLLA
                     GTTVKRASLHNADQIQKLDLHMHDTVFVEKGGEIIPKITGVDFSMRQAGAKPVSYITH
                     CPECGTELIRLEGEANHYCPNEEGCPPQIKGRLVHFTSRKAMNIDGLGVETIDQLVEA
                     ELLKNVADIYHLKSEKLLQLERMGEKTVSNLFEAIEKSKQVPFERVLFAIGIRFVGDT
                     TAKKLARHFGSYQKISQATLEELIQTPEIGDKIATSIFDFVRELENIKMVEQLQSAGL
                     QFKLDKAAMQEAGSKLKGLSFVITGVFQKFERDKLKETIEINGGKVSGSLSSKTSYLL
                     AGSDCGPAKLEKAEKLKINIISEDDFLKMLQ"
     gene            1096..1650
                     /locus_tag="PK134_00540"
     CDS             1096..1650
                     /locus_tag="PK134_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37372.1"
                     /translation="MKIGKAIKQKAASYLFYRELQRQHHQPRTTSFSNARTIGILYDA
                     TIERNYEIVKAYVKHLRDDFKKDVLALGYYDGNELPPMRFSKLGLDFFTKKNLNWHFK
                     PSHPMISKFVNLDFDYLICLNVDRCMPLRYISAATKAGFKIGRYEKGYAGLYDFMISV
                     NGQVTLPQFIDHVNNYLKHIGHDK"
     gene            1647..2522
                     /gene="dapA"
                     /locus_tag="PK134_00545"
     CDS             1647..2522
                     /gene="dapA"
                     /locus_tag="PK134_00545"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRB37373.1"
                     /translation="MNKFTGTGVALVTPFLKNGKIDFNALRKVVNHVIKGGVEYLVVM
                     GTTGESVTLSKEEKKDILKFISEVNNKRTHIVFGVGGNNTLETARELEQIDARMADAI
                     LSVSPYYNKPNQSGIVQHYKTLSGVSQLPIILYNVPGRTGSNLLPQTTLEIAASCKNI
                     IGIKEASGNMEQIMYLIQHRNKGFLVISGDDAITMPLIAAGADGVISVIANAFPKQFS
                     TMVRLALQGKITESRKLHYQLLDLIPLLFAEGSPSGIKYVLSQLDICQNYFRLPVASI
                     SKSLAQKINTVIKGI"
     gene            2549..3562
                     /locus_tag="PK134_00550"
     CDS             2549..3562
                     /locus_tag="PK134_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HRB37374.1"
                     /translation="MGHPAHFHLFKNTIIALQQSGHEAMILIKKKDILEDLLRHAGMQ
                     YININPEGRRDDKFSIALKLLKRDFQFLKICLRFKPQIMIGTSAEIAHVGKLLGIRSI
                     VVNEDDAEVVPWFAKLAYPWATNILAPYCCSVGKWEHKKIGYKGYHELAYLHPSNFSP
                     KKENIKNLGDEPFFILRFAKLTAHHDEGRKGINTLIAEKLINMLSKHGRVYITSEREL
                     ESQFEQYRIAINPLHIHDALYFAQMYIGDSQTMAAEAAVLGTPAIRFNDFVGQISYLE
                     ELEKEFALTFGFKTSDADAMLVHVETLLNQQNLKLQWQEKRSMMLNQCIDLNQFMIKL
                     LTQ"
     gene            3702..4814
                     /locus_tag="PK134_00555"
     CDS             3702..4814
                     /locus_tag="PK134_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB37375.1"
                     /translation="MIFKTDNTEAKLLQLEEFAELDAVLATKAKNDVRNERFVFNLIE
                     KYVDTSRSDCFTFAANNHDDANQLADETYHAVIHFKRVNNFQFINKQFEAINTKLPKG
                     GIFVSKVETNDLRKQRLMEKYPPYLNKVYYFFDFVFKRVFPKLPVTQQIYYAITKGHN
                     RVLSKTEMLGRLYSCGFKVIEERYIGNRLYIVSEKFRDPAYDEDPSYGVIYPMKRVGK
                     GGKIISVYKFRTMHAYAEYLQEYVYEKNNLEEGGKFKDDFRVSRVGRMLRKYWIDELP
                     MILNVLKGDLKIVGVRPLSRHYLSLYSKELQDMRKKFKPGLIPPYYVDLPKTMEEIMD
                     SEMRYLKAYQKSPFLTDVRYFLMVFYTILFKRARSK"
     gene            4874..6517
                     /locus_tag="PK134_00560"
     CDS             4874..6517
                     /locus_tag="PK134_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37376.1"
                     /translation="MKASRLVFILILSFAFQLNISAQYVPQSVFNKPLYDFMDDLANL
                     HIITLNDAIKPYSRKQIAEALQTAESQKEKLNATQQKQLAFYLKDFNKELFTGKGFKK
                     RLDLFYFKDSLFNITVNPVGGFSYYKNENDNMYRQYAGGEFWATLGKGLSIFGSYRDY
                     TDSRAVADDKYLNDLPGYNYKYKGAVGSPKRGGSFDETRGGIAYAWKWGHVGIVKDNF
                     EWGSNNHGANIISTKAPSFARLELKLNPKKWFEFNYFHGWLYSEIKDDSAAYFSGNPP
                     NHRQVYFSKYFAANMFTVKPFKQFDFSFGNSIVYSDQLEVAMFIPFMFFKSLDHSKTG
                     QGSNYSGQNSQLFFNISSHNIKYTHLYISLFVDEIAFGRMMDKNEHSNFYSLKAGAAV
                     MLPFYTSATFIAEYTRTNPVTYRHFVNTTTYESNRFNMGHYLRDNAQEIYLGARCAPI
                     SGLYISAGYTSAAVGPIYPYTGKDKSGLGLPFLKTKEYEFTSLDLNIKYELFNDIFLS
                     AGVKIDDNKGAMANVYTAPFYYGKNGKTTTLFGGFNIGF"
     gene            6520..7044
                     /locus_tag="PK134_00565"
     CDS             6520..7044
                     /locus_tag="PK134_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37377.1"
                     /translation="MNFDNLTTALLIIAPTFIVAGGMYMLIKNMLERDYRLKLLDARR
                     LIQKDMLPLKLQAVERMVVFLERIQPENILFRILQPGMNVRDLQVDLLAAIRQEYEHN
                     VSQQVYVSVQSWSLITKAKDDIIATVNLAADSLDKTEKAIVLSQIILQRSSAGNNFAG
                     AAILSLKSEVDSLL"
     gene            7135..8079
                     /locus_tag="PK134_00570"
     CDS             7135..8079
                     /locus_tag="PK134_00570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB37378.1"
                     /translation="MLKLIPASPNGVTILCFHRISSQYDYFWQPIYPETFRLMLESLV
                     KEYQIIPISQIENKSFRSTKPPLVLSFDDGYKDFIEEAIPVLHYYEAPSNHNIVIDCA
                     DGKSYIWTQKLNHLFNLLRNSSRFSINIALDKNFVLDIKQPCKNWFAHYYKTLFYLFK
                     LDVIDRSECIAVLEQYLNITETDYPIKMMNWDDIKFIATNNVEIGSHTASHVSLSAVK
                     NDVTFEHELNYSKKRIEEMLKCECPVIALPNGLSNGEANQKISEAGYKTVLCLAGSGS
                     NAGTSTDKIRFYNRLNMIQEPNSYMNVRIKLYEKGFIK"
     gene            8076..9047
                     /locus_tag="PK134_00575"
     CDS             8076..9047
                     /locus_tag="PK134_00575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB37379.1"
                     /translation="MSEVQLRQLLKDEIGLLPGLMENCFSMKVDKAYFKWKYLDNPAG
                     NFIGFGAFFENKLVGFYGVIPERYQINNSEVIIFQSGDTMTHSTQRRKGLFEKLATLC
                     YNYVREHHQLAVIGFGGDKSTPGLVKMGWCHIADVKYFFIHALHCKWINNFTTEDRNF
                     NIVEKTDIHVILDIQKNFGFKKNIKQHYSKEFLKWRLANPRFNYRCFICYQGQNPVGY
                     VICYEDEGKLFLLDYCSISVKVQKTLIRRLSQICIKSKLKGIITIAIPKSEEGKWLKK
                     AGFLRNPFKKGPLTTNIPFMILTDQKPESQLCSKDQWQVTPICHDAL"
     gene            9178..11061
                     /locus_tag="PK134_00580"
     CDS             9178..11061
                     /locus_tag="PK134_00580"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRB37380.1"
                     /translation="MKKIFTILTAACLVVGIANAQSQRYVVVEEFTGETCGPCAANNP
                     GFNTKLNANAFTIPIKYQNNIPSNGPNYYSYNTIDVANRTSFYANNYSPHAFLDGNVW
                     DGNAGSFPVATLNSRVATTSPFTVDVTHSFSPTHDIIYTHTVIRATQAVTGLTSLKAR
                     IAITEKDLYGYTSPNGESHYSDVMRKMLPNGTGTILPATWAVGDSVVLDEQWTINVPT
                     NIPTVFMPYWPMLRAVAFVQNDATKEIMQGGRSSAAAVVPLAAITNLTSIFTCTSSID
                     PSVTLKNYGGGGDLTSADIEYGIVGQTMQTYNWSGNLGSNTSVVLTLPNLTLPGAGLQ
                     NFSAKLISVNGNAAFHNINLDYKTSVAQTTAPVTSLSQDFLATTFPPVNWILNNPDNA
                     TTWVRSAAGSLAGGSAKMNFYTYAAAGQSDYLYPYTPIDMTNAVNPQLTFSVAYCQYS
                     TENDRLQVLASTDCGVTWTSLFNEAGAALSTSPAQTTAFTPTSASQWESKTVSLANYV
                     GQPSLLLSFKATNAYGNNLYVDDINLTGVTAVHQIEGVTTLEVFPNPASDVLNIKATM
                     EKSLPMTISLTDISGRKLFQKNLGTVSVVNETVKTSDFAAGIYTLEVRSGDSVSYQKI
                     TIK"
     gene            11119..11820
                     /locus_tag="PK134_00585"
     CDS             11119..11820
                     /locus_tag="PK134_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37381.1"
                     /translation="MKKIVLALILITSASLVKAQSFSYTGLSADVYGSVEDSLLHSYV
                     YVINNSQNTIDASLNAYDVYKVPGTEHNFCWGILCYGVGVMSSAIPATINAGATEGSF
                     RGDYLGHGYVGTTTVAYTVFDGNNPLDSVQFAINYHITPVGVKEVNKTNSISAPSPNP
                     ARSITAFNYNIKSGSKAEVKVINMLGKTVKNFNVNSSTGNIVLSTADLPSGVYFISLN
                     VEGKSSAVQKLVVNR"
     gene            11841..12353
                     /locus_tag="PK134_00590"
     CDS             11841..12353
                     /locus_tag="PK134_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB37382.1"
                     /translation="MNHLKKLVVVFSCAVSVSSAQVFESRPIPAVDVKDLNGKTVNTS
                     TLNNNGKPMVLDFWATWCKPCVNELTAISEHYEEWQKETGVKIVAISVDDARTMANVK
                     PFVNGKGWDYEVYVDPNGDLKRALNIGAVPYVFLVNGKGEIVSVKTGYAPGDEHKMYE
                     EIKALTKESN"
     gene            12374..14017
                     /locus_tag="PK134_00595"
     CDS             12374..14017
                     /locus_tag="PK134_00595"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRB37383.1"
                     /translation="MNKMIKLITGRILPVIFLGAPTLSLAQSSPGEIHGSFQIDGQYY
                     IQDSTISAPEVAEKYLLNGYGDLVFTKGNFKAGVRYETYQNVLLGYDKRFNGSGIAHR
                     YAEYDNGELAITVGNFYEQFGSGLILRSYWEPTLGYDNALDGVRVKFKPYSGILIKGI
                     IGKQRYYFKTGEGIVRGVDAEVGINDLIKKWNDKKTRVTAGFGFVSKYQKDDDNVYIL
                     PENIGAYAGRLDVTRGKVTINAEYAYKYNDPSSLNNYSYKFGDAALLKATYSQKGLGI
                     LLAAKRIDNMNFRSERNANLNDLLINYLPALTKYHTYSLASIYPYATQPNGEFGFEAE
                     VNYTIKKGTKLGGDYGTNIIVNYSGANSLSTEPVAGKDLYKSDWGKFGKDVYYKDFSL
                     EITRKMSKNLKITLMYINQVYDKDKIQFQGSKQYGTIYDNIIVADITFQLNDKNAIRT
                     EWQTLQSEQDYKSWIAGLIEYSHSPNWFVAVSDQYNYGNEKSDKRIHYFNCFVGYTKD
                     SHRITLGYGRQREGLFCVGGVCRQVPSSNGLTVSISTSF"
     gene            14038..15192
                     /locus_tag="PK134_00600"
     CDS             14038..15192
                     /locus_tag="PK134_00600"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRB37384.1"
                     /translation="MKNKFWLAIAALAATTIVSCDKVDGPYKEENTTNIGTLPGTLND
                     IRVLNATTTADDVDVLVAKFSLESSSPKTIAVQFTWHGAQSNVISEVATGNDAIFDND
                     SVYITAPAPFGTLTFKYSATISDTLTGQADFVVPYTPANPVKKVLLEDYTGRKCGNCP
                     RAQRIIANTLEPLYHEQLVVATNHAGVYAVPDNPPSCFSEDFRNSNSDELNTFFGLSA
                     YPSSMINRVGYPTTHIKYYNSWQSVISQELSKPNDAFINIYSAYSESNRTAQLTVSTE
                     FLNNLGDDYKLAVFLVEDSIHGCQLDYDLTTPTQIDSAYIHRHVLRAALNSSFGESLI
                     NDPDLTTKYIRLYNYQIPDKYNVDQCYILAYVYQTNNYYVLQSEIRKVKP"
     gene            15229..15720
                     /locus_tag="PK134_00605"
     CDS             15229..15720
                     /locus_tag="PK134_00605"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRB37385.1"
                     /translation="MLTELYTSYILTPLGYMNAITDTDSVLALEFDDKYTSGEILNGA
                     VSIQIANELQEYFSGKRKLFTLNLKPQGTVFQLQVWEKLLSIPFGKTISYNEQAVQLG
                     SAKKIRASASANGKNPIAIIVPCHRVIGTSGALTGYAGGLWRKEKLLQLESGSQQIEL
                     FSQ"
     gene            complement(15731..18931)
                     /locus_tag="PK134_00610"
     CDS             complement(15731..18931)
                     /locus_tag="PK134_00610"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRB37386.1"
                     /translation="MKRVFTISLCFFVFYSISFAQLDQYASEQNKFYWKNRKPTAGYW
                     QQDVYYNIKAAIDEKTDIISGVEELTYRNNSPDTLKFIFMHLYQNAFVPGGYLDDLNK
                     ANQNKVRYGKYESQGLGIEITNINTLSVNGIIINKQTEKIIDNTIMKIMLPQPLMPGH
                     DLVLKIDFKTYYDKTGQRRRLQMFENAGNKHYNGCQWYPKMCVYDSHRAWNTDQHLGR
                     EFYGEFGVYDVELTFANNFIVEATGVLVNEKEVLPDELRKKLDIKNFKERPASPQPST
                     IIIPDGSTKTWKFHAENVHDFAFTADPSYRLSDAYWNGVRCVAIAREQHCYGWQSAAE
                     YTARIIEVFSRDFGMYDYPKIVTADANDGMEYPMITMDNGTDPGFRGLLCHEIGHNWF
                     YGMVGSNETYRPLLDEGFAQLLKSWGMEMLEGYHIKQRETTWKDQKRTEPLTYQMTSA
                     YGLYYNYVLNDKDGFINTHSDGFSSALGHGGGYGMVYYKTATMLYNLQYILGDSLFLK
                     AMQHYVAQWKFCHPYPEDFRNSITEYVKTDLSWFFDQWMETNKKLDYKICKYRKADEK
                     DSFNVYFKRKGRMQSSIDFSVYSNCGVKYDYHIPNNWFVKQTDATVLPKWEGWDNLRN
                     TYKAQIKIPCGISSIVIDTTDRLGDAYRLDNRLPRPFKLVFDKWQKPAYSDWKSYVFY
                     LRPDVWFNNYDGLKVGVHAESNYMNTMHFFSGDVWINTTVGQNSLSETADINKNDPAS
                     FRINYKTPLKFVAPGVNFWIGGGLLDGLTFAKSGGDLTFLNNRVRLFAEYKLMYRRNT
                     NTLEYLLYPNEWQSALMNTSVTLGAEAQYRKNKWNGSVVIQNTSASLFSDYSYSKLSL
                     TNITNYETGKIDWRLRVFGQYGYGQNVPMESALYLAGANPEELMESAWTRSRAFVPYS
                     WLGYGDKTNHFQQGGGLNLRGYAGYLVPQNIDGEQRYFYRAISGASVSLEIDFDRLIP
                     LRPSFTKKWLKADLYFFGDAGAIATNNSNKSIQFTNLKSDAGIGTAFTIKKFGRLNAI
                     KPLTIRCDFPFLLSSAPYAEKDYVKLRYVIGIGRTF"
     gene            complement(19084..19584)
                     /locus_tag="PK134_00615"
     CDS             complement(19084..19584)
                     /locus_tag="PK134_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB37387.1"
                     /translation="MNISALAEAEKLLFIARQSLSEGKLPQAIDMLNKIVTDYPDFSK
                     AHITLADIYFNKLQDSTTAEEYYKKAIAIADMNTDAFTGYANLMLQQEKYPEALSMLN
                     KAMAIKGAPKDTIYMLLGKLYEMQGRLDDAIGNYKKCITTTLSNSVLEEAELALKRCE
                     VKKKYI"
     gene            complement(19842..20552)
                     /locus_tag="PK134_00620"
     CDS             complement(19842..20552)
                     /locus_tag="PK134_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRB37388.1"
                     /translation="MNIKSIQSLLIVVVLLASCGVANSQKGKLLTPEVFEKMLHETPD
                     AQVIDVRTPEEFNDGHLKNALNMNVNSKDFENRAPYLDKEKPLFVYCYSGGRSAKACE
                     YFEKMGFKVIYDMEGGYSSWTDANLPVEGKKADKAGISLDNFALLTSAGKVLVDINAP
                     WCPPCVKMKPMLDSLDNAWKDKVKIVRLNKDENQLISKSLQINELPTLLFYENGKLVK
                     RESGYKNAEQLKAMTQVE"
     gene            complement(20549..>21046)
                     /locus_tag="PK134_00625"
     CDS             complement(20549..>21046)
                     /locus_tag="PK134_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37389.1"
                     /translation="GMTDNELAEVRNKQIGFIFQTFNLLPRSTALENVALPLVYAGVS
                     KEERLKRAKEVLESVGLADRIMHKPNELSGGQRQRVAVARALVNHPSIILADEPTGNL
                     DSKTSEEIMLLFEEIHQRGNTIIVVTHEEDIARHAHRIVRIKDGLIEDDAINTQITKA
                     KTIVS"
BASE COUNT         6812 a         3621 c         3945 g         6668 t
ORIGIN      
        1 atcgcctaaa tgggcaattg cctacaaata caaagctgaa tcagcttcaa ccattctcaa
       61 tagtgttgta tatcaggttg ggcgtacagg aacaataaca ccggtagcca atttaaaacc
      121 tgttttactc gcaggcacaa cggttaagcg tgcttcattg cataatgccg atcagattca
      181 gaaacttgac ttacatatgc acgacacggt ttttgtagag aaaggtggag aaattattcc
      241 taaaattacc ggagtagatt tctcaatgcg tcaagcagga gccaagccgg tcagttacat
      301 tacacattgc ccggagtgtg gtacagaact tattcggctt gaaggggagg ctaatcatta
      361 ttgccccaat gaagagggat gcccacccca aataaaaggt aggttagtgc atttcacctc
      421 acgtaaggct atgaatattg atggactcgg tgttgaaaca attgaccaac tagttgaagc
      481 cgaactacta aaaaacgttg ctgatattta tcatcttaaa tccgaaaagt tacttcaact
      541 tgaaagaatg ggggagaaga ctgtaagcaa tttattcgaa gcaattgaaa aatcaaaaca
      601 agtgcctttt gaacgtgtac tctttgccat aggcattaga tttgttggtg atacaacagc
      661 aaaaaaattg gcacgtcatt ttggttcata tcaaaaaatc agtcaggcca ctttagaaga
      721 attgatacag acaccggaaa taggagataa aatagcaaca agtatttttg actttgtacg
      781 agagttagag aacattaaaa tggtagaaca actgcaaagt gccggattac aatttaaact
      841 tgataaagct gcaatgcaag aggctggcag taaacttaaa gggttaagtt ttgtgattac
      901 aggagtattt cagaaatttg agcgcgataa attaaaagag acaattgaga taaatggagg
      961 aaaggtttca ggctcgctca gctcaaagac aagttatctg cttgcaggta gcgactgtgg
     1021 acctgctaaa cttgaaaagg cagagaaact taaaatcaac attatctctg aagatgattt
     1081 cctaaaaatg cttcaatgaa aataggtaag gcaataaaac aaaaagctgc aagctattta
     1141 ttttaccgtg aattgcagcg tcagcatcat cagccacgca ccacaagttt cagtaatgcg
     1201 cgcactattg gtattttgta tgatgctact attgagcgta attatgaaat tgtaaaagcc
     1261 tatgtaaaac atcttcgtga cgattttaag aaagatgttc ttgcacttgg atattatgat
     1321 ggcaatgaac tgccgccaat gcgattttca aaacttggac ttgatttttt taccaagaaa
     1381 aatctcaatt ggcatttcaa gccttcacat cctatgattt ccaagtttgt taatctcgat
     1441 tttgattatc tgatttgtct taatgtagat cgttgcatgc cgttaagata tatcagtgct
     1501 gcaacaaaag caggttttaa aatcggtcgt tatgaaaaag ggtatgcggg gctttacgat
     1561 tttatgatta gtgttaatgg tcaggttaca ctaccacagt ttatagacca cgtaaataat
     1621 tatctcaaac atattggaca cgataaatga ataaattcac aggtacaggt gttgcattag
     1681 tgactccttt tttaaaaaat ggcaaaatag attttaatgc gcttcgcaag gtagttaatc
     1741 atgttattaa aggtggtgtt gagtatttag ttgtaatggg tactacaggt gagtcggtga
     1801 cgctttcaaa agaagaaaag aaagatattt taaaatttat tagtgaggtt aataataaaa
     1861 gaacacatat tgttttcgga gtagggggaa acaacacact tgaaactgca cgtgaattag
     1921 aacaaataga tgcccgtatg gcagatgcca ttctttcggt ttctccttat tataataagc
     1981 caaatcagtc aggcatagtg cagcattaca aaacgctatc aggtgtatct cagttgccta
     2041 ttattcttta taatgtaccg ggtagaacag gaagtaatct attgccacaa acaacgcttg
     2101 aaattgctgc aagttgtaaa aacataattg gtataaaaga agcttcaggt aatatggagc
     2161 agataatgta tctcattcag catagaaaca aaggattttt agttatttcc ggtgatgatg
     2221 ctattacaat gccgttaata gctgcaggtg ccgatggtgt aatttctgtt attgcaaacg
     2281 cctttccaaa gcagttcagt acaatggtac gacttgcgct acaaggtaaa attactgaat
     2341 ccagaaaact gcattaccaa cttcttgact taataccatt attgtttgcc gaaggtagcc
     2401 cttcaggaat taaatatgtt ttgtcgcagt tagatatctg tcagaattat ttcaggcttc
     2461 cggttgcatc aatcagcaaa tcattggcac aaaaaattaa tacagttatt aaaggcatct
     2521 gatttagata atgcgttact tgttttatat gggtcatccg gcccattttc atttgtttaa
     2581 aaataccatt attgcattac agcaatcagg tcatgaagca atgattctca tcaagaaaaa
     2641 agatattctt gaagatttgt tgagacatgc aggtatgcaa tacataaaca tcaatcctga
     2701 aggcagaagg gatgataaat tttccattgc attaaaatta cttaaacgcg attttcagtt
     2761 tttaaaaatt tgtcttcgat ttaagccaca gataatgatt ggtacttcgg ctgaaattgc
     2821 acatgttgga aaattactcg gcatcagaag tattgtagtt aatgaagacg atgcagaagt
     2881 tgttccatgg tttgcaaaat tagcttatcc ctgggcaaca aatattcttg caccttattg
     2941 ttgcagtgta ggtaagtggg aacataaaaa aatcggctat aaaggatatc atgaattggc
     3001 atatctgcat ccgtccaatt tctcgcccaa aaaagaaaac ataaaaaatt taggggatga
     3061 accttttttt atacttcgtt ttgcaaaact aacagcacat catgacgaag gccgtaaggg
     3121 tataaatact ctaattgcag agaaattaat aaatatgctt tctaagcatg gcagggttta
     3181 tatcacttca gagcgagagt tagaatctca gtttgagcaa tatcgtattg caataaaccc
     3241 gttgcatatt catgatgcgc tttactttgc tcagatgtat attggcgaca gtcagactat
     3301 ggccgctgaa gctgctgttt taggaacacc tgccataagg tttaatgact ttgttggtca
     3361 gattagttac cttgaagaac ttgaaaaaga gtttgcttta acgtttggct ttaaaactag
     3421 cgatgcagat gccatgcttg ttcacgttga aactttatta aatcaacaga atttaaaact
     3481 gcaatggcag gaaaaacgtt caatgatgct taatcaatgt attgatttaa accaatttat
     3541 gatcaaactt ttaactcaat aacatcttaa aaagccttaa atagttgatt gaattttgtt
     3601 taaaacgatt tgaagcatac acttgcacaa ttcgaaattc gtgcttatct tagcaaacca
     3661 aaatcgaatt attttaaaca tttttcaact ttttttaagt attgattttt aaaaccgata
     3721 acacagaggc aaaacttttg cagttagagg agtttgctga actggatgca gtgttggcta
     3781 ctaaggccaa aaatgatgtc cgtaatgaaa ggtttgtctt taatttaatt gaaaaatacg
     3841 ttgacacatc acgtagtgat tgcttcactt ttgcagctaa caatcacgat gatgcaaatc
     3901 aattggctga tgaaacatac catgcagtta ttcactttaa aagagtaaat aattttcaat
     3961 tcataaataa acaattcgag gcaatcaata ccaagttacc taaaggtggg atttttgtca
     4021 gtaaggtcga aacaaacgac ctgcgcaaac aacgtctgat ggaaaaatat ccaccatatc
     4081 taaacaaagt ttattatttc tttgactttg tttttaaacg ggtatttcca aagctaccgg
     4141 taacacagca aatttattat gcaatcacaa aagggcataa tcgcgtgttg tctaagactg
     4201 aaatgttggg aagactttat tcatgcggat ttaaagttat agaagagcgt tatataggta
     4261 atagacttta tattgtttca gaaaagttca gggatcctgc ttatgatgaa gacccaagtt
     4321 atggtgtcat ttatccgatg aaacgcgttg gtaaaggcgg aaaaattatc agcgtttaca
     4381 aattcagaac catgcatgcc tatgcagaat atctgcagga atatgtttat gaaaaaaaca
     4441 acctcgaaga gggcggtaag tttaaagacg atttcagggt aagccgtgtt ggtcgcatgc
     4501 ttcgaaaata ttggattgat gagttgccaa tgattttgaa tgtacttaaa ggcgatttaa
     4561 agattgttgg tgttcgcccg ttgagcagac attatttaag tctgtattca aaggaacttc
     4621 aggacatgcg taaaaagttc aaacccggac taattcctcc atactatgtt gacctaccta
     4681 aaacaatgga agaaattatg gattcagaaa tgcgctattt aaaagcctat cagaaaagcc
     4741 cattcctgac agatgtacgt tatttcttaa tggtgtttta caccattctt ttcaagcgtg
     4801 ccagaagcaa atagcggaca agtattcctt aaattatttt tttcatcttt gcgcaatcat
     4861 aatttaaaat tttatgaaag caagccgttt ggtttttatt ttgattttgt cttttgcatt
     4921 tcaattgaat atatctgcgc agtatgtacc tcagtcagta ttcaataaac cactttatga
     4981 ttttatggat gacttagcta atcttcatat catcacactt aacgatgcaa taaaaccata
     5041 ttccagaaaa caaattgccg aggcattaca aacggccgaa tcacaaaaag aaaaattaaa
     5101 tgcgacacag caaaaacaac ttgcatttta tttaaaagat tttaataaag aattatttac
     5161 aggtaaggga tttaaaaaac gtttggattt gttttatttt aaagactcgt tgtttaatat
     5221 tacagttaac ccagttggcg gattcagtta ttataaaaat gaaaatgaca atatgtatcg
     5281 ccaatatgca ggtggagaat tctgggcaac tttaggaaaa gggctaagta tttttggaag
     5341 ctatcgcgat tatactgaca gtagagcagt tgcagatgat aaatatctga acgacttgcc
     5401 tggatataat tataaatata aaggtgctgt tggctcgcct aaacgtggag gaagctttga
     5461 tgaaacccgt ggtggcattg cctatgcttg gaagtggggt catgttggaa tagtaaaaga
     5521 taattttgaa tggggcagta ataatcatgg tgcaaacatt atttcaacaa aggcgccatc
     5581 ttttgctcga ttagaactta agcttaatcc aaaaaagtgg tttgagttca actattttca
     5641 cggatggctt tattctgaaa ttaaggatga ttctgcagct tatttctcag gcaatccgcc
     5701 taaccacaga caagtatatt tttcaaaata ttttgctgcc aacatgttca ccgtaaagcc
     5761 ttttaaacaa ttcgatttca gttttggtaa ttctattgtt tatagcgatc aactggaagt
     5821 tgccatgttt attccgttta tgttttttaa atcgcttgat catagtaaaa cagggcaggg
     5881 tagtaactat tcaggtcaaa actctcaatt gttttttaac atcagttcac acaatattaa
     5941 atatacgcat ctttatattt cactatttgt tgatgaaatt gcatttgggc gaatgatgga
     6001 taaaaatgag cattcaaact tttacagtct taaagcaggc gctgctgtaa tgttaccgtt
     6061 ttatacttca gctactttta ttgcagaata tacaagaaca aatccggtta catacagaca
     6121 ctttgtgaat accaccacct acgaatctaa tagatttaat atgggacatt atcttcgcga
     6181 caatgctcag gaaatttatc ttggtgcacg ttgcgcgcca atttcaggtt tatatatttc
     6241 agcaggatac acctcagcag ccgttgggcc tatttatccc tatacaggca aagataaatc
     6301 tggtttgggt ctgccatttt taaaaacaaa agaatatgag tttacatcac ttgatttgaa
     6361 tataaaatac gaattattta atgacatttt tttatctgca ggtgttaaaa ttgatgataa
     6421 taaaggtgct atggctaacg tttatactgc accattttat tatggaaaaa atggtaaaac
     6481 aacaacactt ttcggtggat ttaatatagg attctaaaaa tgaactttga taatttaaca
     6541 acagcactcc tcatcattgc gccaactttt atcgttgccg gtggcatgta tatgctcata
     6601 aaaaatatgt tggaacgcga ttacagactt aaacttcttg acgcaagacg actgatacaa
     6661 aaagatatgc tgccattaaa acttcaggca gtagaaagaa tggttgtatt tctggaacgc
     6721 atacaaccgg agaatatctt attcaggatt ttacaacccg gcatgaatgt gcgagacctt
     6781 caggttgatt tacttgctgc cattcgtcag gagtacgaac ataatgttag tcagcaagtg
     6841 tatgtttctg tacaaagctg gtcattaatc acaaaagcaa aagatgacat aattgcaaca
     6901 gtaaatcttg ccgcagattc tttggacaag accgaaaagg caattgtgct gagtcaaatt
     6961 attttgcaac gttcatcagc aggcaacaat tttgcagggg cggcaatttt atcactcaag
     7021 agtgaggtag atagtttgtt gtaaatctct tttttatacc ttagcccgac aagggttatg
     7081 tctttttcaa cacaaatcat ctttaaaact gctgtaaagc ttcaacttca aaatttgctg
     7141 aagttaattc ccgcttcacc aaatggtgtg acaatacttt gtttccatcg aatttcatca
     7201 caatacgatt atttctggca gcctatttat cctgaaacct ttcgtttgat gctggagtct
     7261 ttagttaagg aatatcaaat tatcccaatt agtcagatag aaaataaatc tttcagaagc
     7321 accaagccac cgttggtttt aagcttcgat gatggctaca aagattttat agaagaagca
     7381 ataccagtgt tacattatta tgaggcgcca agcaatcaca acattgtgat agattgtgcc
     7441 gatggtaaaa gttatatctg gacacagaaa ctgaatcatc tgtttaactt gttgcgaaat
     7501 agcagccgct tttcaataaa cattgcatta gacaaaaact ttgtactcga tattaaacaa
     7561 ccttgcaaaa attggttcgc tcattattat aaaactttat tttatctgtt taaacttgat
     7621 gtaatagatc ggtctgaatg tattgcagtt ttagagcagt atctgaatat tacagaaact
     7681 gattacccga taaagatgat gaactgggat gatattaagt ttatagctac aaataatgtt
     7741 gaaatcggtt ctcatactgc atctcatgtt agtctttcgg ctgttaagaa tgatgtaacg
     7801 tttgagcatg aattaaatta ttccaaaaaa agaattgaag aaatgcttaa atgcgaatgt
     7861 cctgttatag ctttgccaaa tggtttatca aatggcgagg ctaatcagaa aatatctgaa
     7921 gctggttata aaacagtact ctgtttggct ggatcgggaa gtaatgcagg aacttctaca
     7981 gataaaatta gattttacaa cagattaaac atgattcaag agccgaatag ttatatgaat
     8041 gtcagaatta aactttacga aaagggcttt ataaaatgag tgaagttcaa cttaggcagc
     8101 ttctgaaaga tgaaataggg ttgttgcccg gactgatgga gaactgcttc agtatgaagg
     8161 ttgacaaagc ttattttaaa tggaagtatt tagataatcc ggcaggaaat tttattggtt
     8221 ttggtgcatt ctttgaaaat aaattggtcg gtttctacgg tgtcattccg gaacggtatc
     8281 agataaataa tagtgaagta ataatatttc aatccggaga tactatgact cactcaacac
     8341 aaagaagaaa ggggctgttt gagaaattgg caaccttatg ttataactat gttagggagc
     8401 atcatcagtt agcagtaatt ggttttggag gcgacaaatc aactccggga ttggtgaaga
     8461 tgggatggtg tcatattgca gatgtgaaat acttttttat tcatgcattg cattgcaaat
     8521 ggattaataa ttttaccaca gaagatagaa attttaatat agttgagaaa acagatattc
     8581 atgtgattct tgatattcaa aagaatttcg gctttaaaaa aaatataaaa caacattaca
     8641 gcaaagagtt tttaaaatgg agattggcta atccacgatt taattatcgg tgttttattt
     8701 gttatcaagg acaaaatcct gtgggttatg ttatttgtta tgaagatgaa gggaagttgt
     8761 ttttacttga ttattgtagt atatctgtta aggtgcagaa aactttaatc aggagattat
     8821 cacaaatttg tattaaaagc aagctgaagg gaatcataac aattgcaatt ccgaagtcgg
     8881 aagagggaaa atggcttaaa aaggcaggtt ttttaagaaa tccatttaaa aaaggcccgc
     8941 tcacaactaa tattccattt atgattttga ccgaccaaaa gcctgaatca caactttgta
     9001 gtaaagacca atggcaggtg acaccaattt gccatgatgc gctgtaataa ccatagcttt
     9061 aaacactcac ttgcggtctg attttaccag tttattaaaa atggcatttc ttagacattg
     9121 gtattctgaa aagtgtgtac ttttatccaa gaattttaaa atcaaatatt tataataatg
     9181 aaaaaaatct ttactatttt aacggcagcg tgcttagtag tgggtatagc aaatgcccag
     9241 tcacagagat atgtagttgt tgaagaattc acaggtgaaa cttgtgggcc ttgtgcagct
     9301 aataatcccg gtttcaacac caaattgaat gctaatgcat ttactattcc gattaagtat
     9361 cagaataata ttccatctaa cgggcctaat tattattcgt ataatactat tgatgtagca
     9421 aacagaacat cgttttatgc aaataactac tcaccacatg catttttaga tggcaatgta
     9481 tgggatggta atgcaggttc atttcctgtt gcaacattaa actcacgtgt agcaacaact
     9541 tcacctttta cagttgatgt tacacatagt ttttcaccta ctcacgatat tatttatact
     9601 cataccgtaa ttcgtgccac acaggctgtg acaggattaa caagtttaaa agcacgtatt
     9661 gccattactg aaaaggactt atatggttat acttcgccaa atggtgaatc tcattattct
     9721 gatgtaatgc gtaaaatgtt gcctaatggc acaggaacaa tattgccggc aacttgggct
     9781 gttggtgatt cagttgtttt ggatgaacaa tggacaataa atgtacccac taatatacct
     9841 acagtgttta tgccatattg gccaatgctt cgtgctgtag cttttgttca gaatgacgcc
     9901 actaaagaaa ttatgcaagg cggaagaagt agtgctgcag cagtagtgcc tttggcggct
     9961 attacaaatc ttacatcaat atttacttgt accagttcaa ttgaccccag tgtcacatta
    10021 aaaaattatg gaggaggcgg tgaccttact tctgcggaca ttgaatacgg tatagttgga
    10081 caaacaatgc aaacttataa ttggagtggc aatttgggat caaatacatc tgtagtatta
    10141 actttaccta atttaacttt gccaggtgct ggattacaga atttttcagc taaattaatc
    10201 agcgttaatg gtaatgctgc tttccataat ataaatcttg attataaaac atcggttgca
    10261 caaacaacag caccggtaac atcactcagt caggattttc ttgctacaac atttcctcct
    10321 gtaaattgga ttctaaataa ccctgacaat gcaactacat gggttagaag tgctgccggt
    10381 agcttagcag gtggttctgc aaaaatgaac ttttatactt atgctgcagc tggtcaatct
    10441 gactatcttt acccttatac accaattgat atgactaatg ctgtgaatcc tcagttgact
    10501 ttcagtgtag catattgtca atattcaacg gagaacgatc gtcttcaggt acttgcatca
    10561 actgattgtg gagtaacttg gacatctcta tttaatgaag caggtgcagc attatcaact
    10621 tcaccagcac aaaccacagc atttacacca acttcagcat ctcaatggga atctaaaacg
    10681 gtttcattag ctaattatgt aggtcaacca tctttacttt taagtttcaa agcaactaat
    10741 gcttatggta acaaccttta tgttgatgat attaatttaa caggtgtaac agctgttcat
    10801 cagatagaag gtgtaactac tcttgaagtg ttcccaaatc ctgcaagtga tgtattgaat
    10861 atcaaagcaa caatggagaa atcacttcca atgacaattt ccttaactga tatttcagga
    10921 agaaagttgt tccagaagaa tctcggcact gtttctgtag taaatgaaac tgtaaaaact
    10981 tctgattttg ctgcaggaat ttatacactt gaagtacgtt caggcgattc agtttcttat
    11041 caaaaaataa caattaaata attaaatcaa ttgctgtccc ggtatctaag ataacggggc
    11101 agctttaaaa aaaatatcat gaagaaaatt gtactagcac taattttaat tacatcagca
    11161 agtttggtta aagctcaaag ttttagctat acaggattat ctgcagatgt ttatggctct
    11221 gttgaagaca gtttattaca ttcttatgta tatgtaatta ataacagtca gaatacaata
    11281 gatgccagcc tgaatgccta tgatgtttac aaggttcccg gaacagagca taacttttgt
    11341 tggggtattt tatgttacgg agtaggtgtg atgtcatctg caatacctgc tactattaat
    11401 gcaggtgcaa cagaaggatc attcagaggt gattatcttg gacatggtta tgttggtact
    11461 actactgtag cctatactgt atttgatgga aacaatccac ttgattcagt tcagtttgcc
    11521 attaactatc atattacacc ggttggcgta aaagaagtaa ataaaacaaa tagcataagt
    11581 gcgccatctc ctaaccctgc acgttcgata actgcattta attataatat taaatcaggt
    11641 agcaaggctg aagttaaagt aataaatatg ttgggaaaaa cagttaagaa ttttaatgta
    11701 aatagctcaa caggaaatat tgtactttca acagccgatt tgccatctgg tgtttatttt
    11761 atttcgctta atgtagaagg aaaaagttca gctgtacaaa agttagtagt aaacagataa
    11821 aattcaattt taataaataa atgaatcatt taaagaagtt agtagtagtc ttttcatgtg
    11881 cagtatcagt ttcttcggca caggtttttg aatcacgtcc aatacctgct gttgatgtaa
    11941 aagatctgaa tggtaaaact gtaaacacgt caacattaaa taacaatggc aaaccgatgg
    12001 tgcttgattt ttgggcaaca tggtgtaagc cttgtgtgaa tgaacttaca gccatcagtg
    12061 aacattacga ggaatggcag aaagaaacag gagttaaaat tgtagcaatt agtgttgatg
    12121 atgcacgaac aatggcaaac gttaaacctt ttgtcaatgg caaaggttgg gactatgaag
    12181 tttatgttga ccccaacggt gatctgaaaa gagccctcaa cattggagca gtaccttatg
    12241 ttttcttagt taacggaaaa ggcgaaattg tttcagtaaa aacaggctat gcacccggtg
    12301 atgaacataa aatgtacgaa gagattaaag cacttactaa agaatcaaat taataaataa
    12361 ctatttgctt ttcatgaata aaatgataaa acttataacc ggcagaatat tgccggttat
    12421 ttttttaggt gcacctactt tatcgcttgc acaaagttct cccggtgaaa tacatggtag
    12481 ttttcagatt gatggacagt attacattca ggattcaact ataagtgcac ctgaagtagc
    12541 agagaaatat ttgcttaatg gttacggaga tttggttttt accaaaggta atttcaaagc
    12601 aggtgttcgt tacgaaactt atcagaatgt attgcttggc tacgacaaaa gatttaatgg
    12661 cagtggtatt gcgcatcgtt atgcagaata tgacaatggc gaattagcta ttactgtcgg
    12721 aaatttttat gagcagtttg gcagtggatt gattttgcgt tcctattggg agccaacact
    12781 aggttatgat aatgcattgg atggtgttcg tgtaaaattt aaaccttaca gtggaatcct
    12841 tattaaaggt ataattggta aacaacgcta ttattttaaa accggagaag gcattgtcag
    12901 aggagtagat gcagaagtgg gtataaatga tttaataaaa aaatggaatg ataaaaagac
    12961 cagggttact gcaggttttg gatttgtgag taagtatcag aaagatgatg acaatgtata
    13021 tatcttgcct gagaacattg gtgcttatgc cggacgactt gacgttacaa gaggtaaagt
    13081 aaccatcaat gcagagtatg cttataaata caacgaccct tcatcattaa ataattacag
    13141 ctataagttt ggtgatgccg cacttttgaa agcaacttat tctcaaaaag gtttgggaat
    13201 attacttgcc gccaaaagaa ttgacaacat gaatttccgt agtgagcgta atgcaaatct
    13261 aaatgactta ttgattaatt atcttcctgc attgacaaaa tatcacacgt attcattagc
    13321 atcaatctat ccttatgcaa cacaaccgaa tggtgagttt ggatttgaag ctgaagtaaa
    13381 ctatactatt aagaaaggaa caaaattagg tggtgattat ggaacaaata tcattgttaa
    13441 ctattccgga gccaattcat tgagcacaga gcctgttgca ggcaaagacc tttataaaag
    13501 tgattgggga aagtttggta aggatgttta ctacaaagat tttagcttag agattactcg
    13561 taagatgtct aaaaatctga aaattacttt aatgtatatt aatcaggttt atgataaaga
    13621 caaaattcag tttcagggat caaagcaata tggaactatt tatgacaata taatagtagc
    13681 tgatattacc tttcagttaa atgataaaaa cgccatcaga acagagtggc aaacattaca
    13741 atcggaacaa gattataagt cttggattgc cggactgatt gagtattcac actcaccaaa
    13801 ttggtttgtt gcagtatcgg atcagtataa ttatggcaat gaaaaatctg acaaaagaat
    13861 ccattacttc aattgttttg tcggatacac aaaagattcg caccgtatta ctttgggtta
    13921 tggtcgtcag cgcgaaggtt tgttttgcgt tggtggtgtg tgcagacagg ttccgtcaag
    13981 taatggactt acagtttcaa taagtacaag tttttaatta tttaaaagtt cacgaaaatg
    14041 aaaaataaat tttggttagc aattgcagct ttagctgcaa caacaattgt ttcatgcgat
    14101 aaagttgatg gtccttataa agaagaaaac acaactaaca ttggtacact tccaggaaca
    14161 ctcaatgaca tcagagtgct taatgcaacc acaacagctg acgatgtaga tgttcttgta
    14221 gctaagtttt cactcgaatc atcatcgcct aaaactattg cagttcagtt tacatggcac
    14281 ggtgcacaat ctaacgtgat ttcagaagtg gcaacaggca atgatgccat ctttgataat
    14341 gattcggttt acataacagc tcctgcgcct tttggaacac tgacatttaa atattcagcc
    14401 actattagcg atacgctaac aggtcaggct gattttgtgg ttccatacac ccctgcaaat
    14461 cctgttaaga aagtgttact cgaagattat acaggacgta agtgtgggaa ctgtccgcgt
    14521 gcacagagaa taattgctaa cacacttgag ccattatacc atgaacagtt ggttgttgca
    14581 accaaccatg ccggtgttta cgcagttcct gataatccac caagttgctt ttctgaagat
    14641 ttcagaaatt ccaactccga tgagttgaat actttttttg gtttatctgc atatccttca
    14701 agtatgatta acagagtagg gtatccaaca acacatatta aatactacaa cagttggcag
    14761 tctgttatat cacaagagtt aagtaaaccg aatgatgcat ttataaatat ctattctgca
    14821 tatagtgaaa gtaatcgcac agctcaactt acagtaagta ctgagttttt gaacaacctt
    14881 ggcgatgatt ataagttagc agtattttta gttgaagaca gtattcacgg atgtcagctc
    14941 gattatgatt tgacaacacc tacgcagata gattcagcat atatacacag acacgtactt
    15001 agagctgcat taaatagttc ttttggtgaa agcctgataa acgatccgga tctgacaaca
    15061 aagtatatca gactttacaa ctatcagatt cctgataagt ataatgttga tcagtgttat
    15121 attctggcct atgtttatca gaccaacaac tattatgttt tgcagtctga aatcagaaag
    15181 gtaaagcctt agggattttc aatttgtaaa aatcaattat tgccattatt gttaactgaa
    15241 ttatatactt cgtatatcct gacaccgtta ggttatatga atgctatcac agatacagat
    15301 agtgtgcttg cacttgaatt tgatgacaaa tacacttccg gtgagatatt gaacggagct
    15361 gtgtcaatac agattgcaaa cgaactacaa gagtattttt ccggtaaacg taagttattc
    15421 accttaaatt taaaaccaca gggaacagta tttcagcttc aggtttggga gaagttactt
    15481 tctataccct ttggaaaaac catctcttat aacgagcagg cagtgcagtt agggagtgcg
    15541 aaaaaaataa gagcatcagc atctgccaat ggtaaaaatc cgattgcaat aattgttcct
    15601 tgtcatagag ttattggaac ttcaggtgca ctaaccggtt atgcaggcgg tttgtggcga
    15661 aaggagaagt tgctgcaact tgaatcggga agtcagcaaa tagaactttt cagtcaatag
    15721 cgtgatgtta tcagaaagtg cgaccgatgc cgattacata tcgtagtttg acataatctt
    15781 tttcagcata aggggcactg cttaacaaga atggaaaatc acatcgtata gttagcggct
    15841 ttatggcatt caaacgacca aattttttaa ttgtaaatgc tgttccaatg cctgcatcac
    15901 ttttcaaatt agtgaattga atggacttgt tagaattgtt tgttgcaatg gcacctgcat
    15961 ctccaaaaaa ataaaggtca gcttttaacc actttttagt aaatgatggg cgtaaaggaa
    16021 tcaacctgtc aaagtctatc tctaaactaa cagaagcacc actgatggca cgataaaaat
    16081 atctttgttc gccatcaata ttctgcggca caagataacc tgcataaccc cttaaattaa
    16141 gtccaccacc ttgctgaaag tgattggtct tgtctccata acccaaccaa ctgtaaggaa
    16201 caaatgcacg tgaacgtgtc caagcacttt ccatcaactc ctccggattt gctcctgcaa
    16261 gatatagagc actttccata ggaacatttt gtccatatcc atattgaccg aaaactctta
    16321 atcgccagtc aatttttcct gtttcgtagt ttgtgatgtt agttaatgaa agcttagaat
    16381 aggagtaatc actaaataat gatgctgaag tgttttgtat gacaacagaa ccattccatt
    16441 tattttttct gtattgtgcc tctgcaccta aggttacaga tgtattcatc aaagccgact
    16501 gccattcatt cgggtataat aaatattcaa gcgtgttggt gtttcttctg tacatcaatt
    16561 tgtattcagc aaacagtctt acacgattgt ttaggaaagt taaatcaccg cctgattttg
    16621 caaatgttaa accatcaagc agtccgccac caatccaaaa attaactccc ggtgcaacaa
    16681 actttaatgg tgttttgtaa tttattctga atgatgctgg gtcattttta tttatgtcgg
    16741 cagtttcgct caaactattt tgtcccacag ttgtattaat ccaaacatca cctgaaaaaa
    16801 agtgcatggt attcatgtaa ttactttctg catgtacacc aacttttagt ccgtcataat
    16861 tgttgaacca aacatcagga cgtaagtaaa atacatagct tttccagtct gagtaggcag
    16921 gtttctgcca tttatcaaac acaagtttaa aaggtcgcgg caagcggttg tccaacctat
    16981 aagcatcacc taaacggtct gtcgtgtcta taacaatact gctaatacca catgggattt
    17041 taatttgagc cttataagta tttctcagat tatcccaacc ttcccatttc ggcaataccg
    17101 ttgcatcggt ttgttttaca aaccagttat tgggaatatg ataatcgtat tttacaccac
    17161 agtttgaata gacagaaaaa tcaattgatg attgcatgcg ccctttgcgc ttaaaataaa
    17221 cattaaaaga gtctttttca tcggccttgc gatacttgca gattttatag tctaattttt
    17281 tattggtctc catccactga tcaaaaaacc agcttaaatc ggttttaaca tattctgtga
    17341 tgctgtttct gaaatcttca ggatagggat gacaaaattt ccattgtgca acatagtgtt
    17401 gcatcgcctt taaaaacaaa ctgtcaccta aaatatattg caggttgtat aacatggttg
    17461 ctgttttgta ataaaccatt ccataaccac caccatgacc taatgcgctg ctgaaaccgt
    17521 cactatgtgt attgataaat ccatctttat catttagaac ataattgtaa tagaggccgt
    17581 atgctgatgt catctgatag gttaaaggct ctgttctttt ctgatctttc catgttgttt
    17641 ctctctgttt tatatgatag ccttcaagca tttccattcc ccacgatttc agtaactgcg
    17701 caaatccctc atcaagcaat ggcctatagg tttcattact tcctaccata ccataaaacc
    17761 aattatgtcc tatttcatga catagcaggc ctctgaatcc gggatctgta ccattgtcca
    17821 ttgttatcat tggatattcc attccatcat ttgcatcggc agtaacaatt ttcggataat
    17881 cgtacattcc aaaatcgcgt gagaaaactt caataatacg tgctgtatat tctgccgcac
    17941 tttgccatcc gtagcaatgt tgctcgcggg caatagctac acaacgaaca ccattccagt
    18001 aagcatcaga aagacgatag gaaggatcgg cagtgaaagc aaaatcatga acgttttctg
    18061 catggaattt ccaggttttc gtacttccgt cagggatgat tattgttgaa ggttgtggtg
    18121 aagcaggtct ttctttaaaa tttttaatat caagtttctt gcgcagttca tcaggtaaaa
    18181 cttctttctc attgactaaa acacctgttg cctctactat aaagttgttt gcaaaagtaa
    18241 gttccacatc atacacacca aattctccat aaaattctct gccaagatgc tgatcggtgt
    18301 tccaagcacg atgcgaatcg taaacacaca ttttaggata ccattgacat ccattgtaat
    18361 gtttgttgcc tgcattttca aacatctgta agcgtctgcg ctgaccggtt ttatcgtaat
    18421 aagttttaaa atcgattttc aaaacaagat catgacccgg catcaaaggt tgcggcaaca
    18481 taattttcat gatggtgttg tcaattattt tttcggtttg tttatttatt ataataccat
    18541 tgacagaaag tgtatttata ttagtaattt caattcccaa tccttgtgat tcatattttc
    18601 cataacgcac tttgttttga ttggctttgt ttaagtcgtc caagtaacca ccgggaacaa
    18661 atgcattctg atagagatgc ataaaaataa atttcaaagt gtcgggagaa ttatttctgt
    18721 aagtaagttc ttctacacca ctgataatgt ctgttttttc gtctattgca gctttaatgt
    18781 tataataaac atcttgttgc cagtagccag cagtaggttt acgatttttc caataaaatt
    18841 tattctgttc agaagcatat tggtctaact gagcaaaact tatagaataa aatacaaaaa
    18901 aacacagtga aatggtaaag acacgtttca ttaatttaca gttgtagttt aaaggcaaat
    18961 gtaatgtatt cgtgaaaatt tttggcttac cctgaaatgg ggtttttaaa ttctatctgt
    19021 aagttgtaaa gcagaataga ataggttgca aagcactaaa aacaacgttg taaatagaaa
    19081 aaattaaata tactttttct tgacctcaca tcttttcaat gcaagttctg cttcttcaag
    19141 aacggagttt gatagggtag tggtaataca ttttttatag tttccaatag catcatcgag
    19201 tctgccttgc atttcgtaaa gtttgcccag cagcatgtag atagtatctt ttggagcacc
    19261 ttttatagcc atagctttgt tgagcattga caatgcttcg ggatattttt cctgttgcag
    19321 cattaagttt gcgtagccgg taaaggcgtc agtattcata tcggcaatgg caatagcttt
    19381 tttataatat tcttctgcag ttgttgagtc ttgaagttta ttaaagtaaa tatctgccag
    19441 agtgatgtgt gcctttgaaa aatcaggata gtcggtaacg attttattga gcatatctat
    19501 tgcttggggc agtttccctt ctgaaagcga ctgccttgca ataaatagaa gtttttcggc
    19561 ttctgctaat gcagatatat tcataatttg ctcaacaact aaatgacttt atacgtagtt
    19621 atgcccttta ggttacacgt tttatatgtc tgaaaaagcg caatttacac ttttgaaaag
    19681 gagaaggcaa gactaaaaaa atattaattc tatgtgttct gaatcagtga gataggtcaa
    19741 tatgatttag cagagaaggc gtttttaata ttcgggattt attaatgaat tattctaatg
    19801 caatctcatt taaaagtaga ataaaaagta aatccactca actattcaac ctgagtcata
    19861 gcctttagtt gttcagcatt tttatagcca ctttcacgtt tcaccaattt accgttttca
    19921 taaaaaagta aggtaggcaa ttcatttatc tgaagcgatt ttgaaatcaa ttgattttcg
    19981 tctttattca atcgcacaat ttttactttg tctttccatg cattatctaa tgaatctaac
    20041 ataggtttca tttttacaca tggcggacac catggcgcat taatatcaac caaaactttc
    20101 ccggcacttg taagcaaggc aaagttatca agactgatac cggccttgtc agctttttta
    20161 ccttctacag gcaggtttgc atctgtccat gaagaatagc caccttccat atcataaatt
    20221 actttgaatc ccattttctc aaaatattcg caggctttgg cactgcgacc accggaatag
    20281 caataaacaa aaagaggttt ttctttatcg agatagggtg cacggttttc aaaatctttg
    20341 ctgttgacat tcatgttcaa agcatttttc agatgcccat cattaaattc ttcaggtgtt
    20401 cttacatcta taacttgtgc atccggagtt tcgtgaagca ttttttcaaa tacttcaggt
    20461 gtgaggagtt ttcctttctg actgtttgct actccacaac ttgctaatag cactacaact
    20521 ataagtagcg attgaattga tttgatgttc atgatacaat tgtttttgct tttgttattt
    20581 gtgtgttgat ggcatcatct tcaatgagtc catcttttat tctgacaata cggtgtgcat
    20641 gtctggcaat gtcttcttca tgggttacca caataattgt attgccacgc tgatgtattt
    20701 cttcaaatag caacataatc tcctcagagg tttttgaatc aagatttcct gttggctcat
    20761 cagcaagaat gattgaaggg tggttgacca atgcccttgc aacagccaca cgctgacgct
    20821 gaccaccgga taactcattt ggcttatgca taatcctgtc ggccaagcca acactttcaa
    20881 gtacttcttt tgcccgcttt aaacgctctt cttttgaaac acctgcatac acaagcggta
    20941 aggctacatt ctcaagtgct gtacttcgtg gcaacagatt aaatgtttga aaaataaaac
    21001 ctatttgctt atttcttact tcggcaagtt cattatctgt cattcc
//
LOCUS       DAORQT010000007        20984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_171_length_20984_cov_8.884658, whole genome shotgun sequence.
ACCESSION   DAORQT010000007 DAORQT010000000
VERSION     DAORQT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20984
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_20984_cov_8.884658"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5819
                     /locus_tag="PK134_00630"
     CDS             <1..5819
                     /locus_tag="PK134_00630"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRB37390.1"
                     /translation="VIPAYPSTCWYEDPSSGDWITTNNSSSSNDADARTGAQFLRDAY
                     SATNEFIWTPGFSLTAGQSVDFSFWWAGDNYSGWTGDVFYNTAQTGTGATQMGTSFVT
                     SGTTTTKTYAKQTRTLVAPATGTYYFAIRVNATGGPWYLSFDDFNVENTPSCVAPTAL
                     VGTPTTATTANISWTAASPVPSNGYEYAVTTSATPPGSGTAFAGTSTSVSGLTPSTTY
                     YLHVRSYCGGSSYSTWASSASFMTGQIPATLPYSNSFGSNDFAFVNGSQTNQWAYGSA
                     TGNPANAIYVSNDNGTSNAYTISSLSVVQAFRDIAIPNGTTLTNFSFNWIANGESCCD
                     YLRVWLVPTSYNPTAGTQITAGAGRIQIGSNMNVQSAWQTYTNNTLDLSSFAGQTMRL
                     VFEWKNDGSIGTQPPAAIDNISLAQVASCSGTPAPGNTQSNLPSACSAQSFTLSMSNT
                     INDIGITYDWQSSPDGITWTSTGGTNATYTTTQTAATYYQCVVTCTNSGMSATSTPVQ
                     VTMSPFYNCYCTTNLHTSSSPCITQVDFNTISNNTAGAGCALPAYSYQAATTTVLKGS
                     SYTFTRTTTSTGVWTGVWIDYDQSGTFDASEYTEVSSTSTGALTNSVTITIPVTALDG
                     NTGMRIRQRTVDMLATDACTQGFGSGETEDYTVDIQTPVGCSGTPSATIGGAPANVCA
                     GVPFTLTAGPTGLAGFTYQFQNSTDGGLTWNNMGIADVFNNYIVSNQTVTTMYQVIVT
                     CTNSGLSNTSTAVTVTQNSPTACYCTPTTVNGFTYYITNFTTTGGITNINNSSVGGGP
                     YQNFSGSASASANPGATINYSISVTSGTYGRAIWIDLNEDGVFQATEQLVTSSSFLSS
                     PLTGSFIIPNGTTAGTKRMRVLASWSYSGPTDPCNISGNGEYEDYSFEVVALPSCTGT
                     PAATISGPANICAGIGFNLTAGPTGVDGLTYQFQNSTDGGITWNNLGSAGINNIYAIT
                     SQSVTTQYQVIVYCSYSGLNATSTPITVTQNLPSACYCTPTTGGGLTYYISNFTTTGG
                     TTNINQSSGGSSTGYDDHSATVSGEFEPGSTINFTMTIAGSSNFGHAIWIDFNADGTF
                     QATEQIVTSNSYLYSPITGSFTIPLNATTGVRRMRILTSFTPSNPTNPCTNSGQGEYE
                     DYSINITPPVCISAPSSPANAGSACADVITNTVLLSWPSLSGATGYDVYFDANNPPTT
                     LVSANQGGLSFSADVSGGAPVYYWMVVPQILGGGSSCTVWSFSLSPSPTPIASSGGDV
                     CLGNDISLTGDNIAMGQSSGNTYSWTGPNGFNSTLQNPTIINPGVLYSGTYTVVVTNQ
                     YGCSASESTSLSVNDNPTLSIDSLKNVGCIGGSDGIIYVSASGGLSPYSFTSDFINFN
                     VDGIMNNLPTGMSTIYVADGNACQAQISGSLTYTSTAPPAQSVNVPILGMPAYACNGT
                     TATISVAAVANATQYTWDGPVGTTFNGNPSPYISTTPSVTVVFGAPNGSGYRIGVQAG
                     NGCGSSLRKSQWLRGIVSTPAAVSGATTTCANSSGTYSTVAVDGATSYLWTVSGDATV
                     SGNGTSVTVNFGPSWTGGTLCVSAQTPCFNSPSKCLVIGSSASPLNVISGSFTACPNQ
                     TLTFSVPASTGAASYNWTLPAGATGSSTTNSINATFSPSYNSVGNICVSVTSICGVTS
                     PIKCKTVAPGLPSVPPAITGITNGVCGQSVSYSVAPVSGITYNWTAPGTVIGNGNSTV
                     SVQYGSLTTGQVCVTASNSCGTSASRCVSVKGTPNSPIALTAVPSSWCANTQGIEFTA
                     DMSNTTGSYTISWTYPSAPTASYVAGGGNSNALTLDWGTGNGSVVVTATNSCGAGSKA
                     FAVTIGCKEGEMASASKLNVYPNPTAGVLNVEYTADKGTAQVTVLDLSGRVVMTQSHA
                     NAAGQNILQLDLSKVAKGAYMLNVQTEGSNNQVRVVVE"
     gene            complement(5917..7011)
                     /locus_tag="PK134_00635"
     CDS             complement(5917..7011)
                     /locus_tag="PK134_00635"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HRB37391.1"
                     /translation="MWRNLTTTFIISLFTVNSLTASKLQPGYNPKEYMELLSFQLMQF
                     HKQDSLYPDSMHYHNFKEVYKSPEMGLLNQWSLWMGADSVAVISIRGTIANSTSWLAN
                     FYAGMIPAKGQLILAPGDTFNYHFADDSKAAVHTGWTLATAYLIRDILPHIDSCYKKG
                     ITNFIVSGHSQGGAIAFLVTSCLYDYQKNNQIASDIRFKTYCSAAPKPGNLYFAYEYE
                     NMTRNGWAFNVVNSADWVPETPITIQTTKDINNTNPLVNAKAGIRKQKFPANLFVMHI
                     FNRLQKTPYKAMRQYRKYLGGFAGKLVRKNLVNFQSPIYLNSSDYVRTGTTIVLLADK
                     NYFDLFPDSKTKIFVHHFYEPYLYLAAKRQ"
     gene            complement(7123..9045)
                     /locus_tag="PK134_00640"
     CDS             complement(7123..9045)
                     /locus_tag="PK134_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRB37392.1"
                     /translation="MINNKSSHWLFQRFAGIFSRIYSSKLNPLYHLGDIAILMFIVAC
                     ISGVYLFLFYNIDPAHAYESVEKISANPFNAVIRSIHRYSSDLLVIFIVLHFLQTLLT
                     GKFKRILSWVSGIISLMVVLIIGVTGFILVWDEKGKLVGYLTAKFFSAIPLFDPSIAG
                     SFISNNLDVIGGFFKVSVFGHIFFSIFLVIVLWLHVMRIAKPKLFSPRIIWIYSMIAL
                     VLVCLVFPVKSDPSAQERFIPYNTTFDWYYFFGYSFMKVVSVPMNWVIMLVSGAGLAL
                     VPLIFKKKNKPATIDLDKCDACNICSEDCPYGAIDMLIYKGERKAILDPDKCISCGIC
                     LASCKEHAITMPGIPEHNVLPLNLAQKSQLSIVSCSQFDDVKIPDGITHKKKSLPCLG
                     DLHMHEVEQMLNEESEGVLLLGCEDCYYRYGRNLAVARINRKRPPGLAKKLSRDRIQL
                     ITTNHYSAKAIREFTDSLASAKPNDGKATIKVLDYFKTNHIAAALILCAFFLLMPFFS
                     NTKLSFFDKDEKLLVLNFKYISSPTEFQEQNTNQQRQMQSLKPIVKRRSPIMVEVAGS
                     DGNILLKKEFSPRGLRKDISIFVYSEIPTTDSLVNIKVTETAFPDKVVSLQNVALSTI
                     DGTIISFADGKLKQLK"
     gene            complement(9042..9818)
                     /locus_tag="PK134_00645"
     CDS             complement(9042..9818)
                     /locus_tag="PK134_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRB37393.1"
                     /translation="MINNKMQLTGLVILLSLFFSLSSFDIPDEKKFIGREVKNIPILD
                     SKGNNLQLFDYPTGKPIVLSPVYTRCPMACSLISNGLKTSIEKLGTLGKDFTVISFSF
                     DSTDTKEDLSGFEARWKMDGVNWKTVTSNAAGIGELLRSIDYHYEYDEKSKEYLHPNL
                     VVVITPSGRISRYIYGVEPKAIDLELAIMEAQVEKTSFNNFFKTLYIRCFTFNPATKS
                     YHLDWKFISQTSVGMMFIIIVGYFLIKSLYSNNTEDSAEL"
     gene            complement(9849..11516)
                     /locus_tag="PK134_00650"
     CDS             complement(9849..11516)
                     /locus_tag="PK134_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRB37394.1"
                     /translation="MFRICDITGFKVDLQSEKFIRLNAVMAVVSLLLAITAAILLVFT
                     RYQPVHLLDAEWYYRLTTFHGLNALIYWIVFFEIAGLYFGSTVVLNQKFCSPTLGWVT
                     FLLMVGGMVLSNVMVLMGKADVMLTSYVPLKAHPLYYLGIILFAVGALIGVGLFFANI
                     VNAKRDGNVGETLPLFTYGLMTAAILAVITLASGAIIYIPTFLWSLGLIDSVDPAVYK
                     LIWWGLGHSSQQINVAAMVSIWYLVTFLALGGTSINERVSRTAFFLYILFISLASAHH
                     LLTDPATSSAWKVWNTSYAMYLAVLASMIHAFAVPSSIESAQRRWGFNKGLFEWLSKA
                     PWSNPAFAACVLACIGFGFIGGTTGVIFGMEQTNIIVHNTIAIPGHFKGTVVIGTTLT
                     FMGITYYLIPLIFRRKIVAFKLAQLQPWMFFFGVAILSVSMIALGQLGVPRRHWDSTF
                     SGGPFTHEFNPSVDFFWTLMMLGGTLAFLAALIWVLQVVVSVFFGPKVNGPEDMQLPL
                     SPMAPAAKEHKGYEAPGTLILTLLFLATFAVLFFVNWKWLAATWWVK"
     gene            complement(11529..12458)
                     /locus_tag="PK134_00655"
     CDS             complement(11529..12458)
                     /locus_tag="PK134_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB37395.1"
                     /translation="MTLIAPTEGWFKKKVSKDERMWIIISFVVCIFLFAWMVLWHVFG
                     RQNPSNTTYKTNPIEFAQLHDEYVKRNMVGMDNGIPIVKPEPGSDVILMAQMWRWSPV
                     LILKKDEWYNIHISSKDLVHGFSLQPSNMNFQVYPDYDYVLKFKPTVAGEAKVLCNEF
                     CGIGHHTMIGKILVIENDADLAKYGYNNLSAPPARTGKGTAEEDISGWSEEQLIKGGE
                     MVYNLKGCMACHKTDGTSEIGPAFNSIYGSKVKVKEDGNAKEIVIDDDYITTSIREPQ
                     KQIVVGFEKTPMAQMNLTDNEIKQVISYIKSIK"
     gene            complement(12479..12658)
                     /locus_tag="PK134_00660"
     CDS             complement(12479..12658)
                     /locus_tag="PK134_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37396.1"
                     /translation="MEEQENKKKASGLQVVMDDVMLLLFLGVTIYAIFYIIWGIMEIA
                     TVPNIPESVKQSILK"
     gene            complement(13020..14042)
                     /locus_tag="PK134_00665"
     CDS             complement(13020..14042)
                     /locus_tag="PK134_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRB37397.1"
                     /translation="MGLFDFLTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRMTGK
                     VLAVGKQAMMMHGKTHENIKTIRPLKDGVIADFDAAENMIRGMIKMINPGRRFFQPSL
                     KMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDGFTMDIWDYMRRQHNILIGERSAEMIKIEVGS
                     ALPELDNPPHDYAVHGRDLMTGIPKEITVTYSEIAHALDKSISKIEEAILKALEMTPP
                     ELSADIYRTGIYLTGGGALLRGLDKRIGMKTKLPVHVAEDPLRAVVRGTGIALKNIDR
                     YKFLIP"
     gene            complement(14165..15694)
                     /gene="purH"
                     /locus_tag="PK134_00670"
     CDS             complement(14165..15694)
                     /gene="purH"
                     /locus_tag="PK134_00670"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRB37398.1"
                     /translation="MKIKSALISVYNKNGFEPLIKALHQQQVTLFATGGTFDFIQSLK
                     IPITAVEDVTDYPSIFGGRVKTLHPKIFGGILNRRNDANDVMEVEKYNIPCIDLVVVD
                     LYPFEATVASGADESAIIEKIDIGGVSLIRAAAKNFNDVSIIASADDVTAFLQTYNQG
                     NGEVNLSFRKKLAQKAFAITSHYDSAIFNYFYSKNEQSEPVLNLQCNNSVTLRYGENP
                     HQRGYYFGNLNETFVQLGGKELSYNNMLDCDAAINLIAEFSNEKGVTAAIIKHNNACG
                     IATREKATDAWQAALAGDPVSAFGGIIVFNQHIDESLAKEINAIFFEVIIAPSFAEEA
                     VKVFSEKKNRIILQLKKQLVAKQSIKSALGGYLMQETDSEALSTDGWNNITLRKPTTT
                     ETEDMLFAVRVIRNLKSNAITLVKNKQLIGSGAGQTSRVDALKQAIAKAVNFGFNLKN
                     SVMASDAFFPFPDCVEIAKTAGIEAVVQPGGSIKDKDSIEYCNKNNMTMVLTGKRHFK
                     H"
     gene            15889..17079
                     /locus_tag="PK134_00675"
     CDS             15889..17079
                     /locus_tag="PK134_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37399.1"
                     /translation="MLRTVLSLLGISIGIFAIISVFTVVDAMEKNVRTSVESLGDKTI
                     YVQKWPWAFGGDYPWWKYWQRPLPSVKEMHQLAARSQSIEAFTFEAYLNGKTVKYLNN
                     SAENVTVALVSHDFDKVKSFELSAGRYLTEMESNSGRAVGIIGSNVANGLFGSASNAL
                     GKVFEVMGRKVEVIGVFKEEGKSLLETEMDNTVVLPLNFGRNFVNIRSDRIDPFIKAK
                     VKEGVPIQFANDELKSIMRSIRRLRPIEDDNFALNQSSLLSNNITNIFSALNMAGAII
                     GGFSILVGGFGIANIMFVSVKERTSQIGIQKSLGAKNYFILMQFLFEAIVLCLIGGLI
                     GLSIIYFLAMFVSHAFDYPLSLTGHNIMLGLFISALIGLVSGFIPAYSASQLDPVEAI
                     RANG"
     gene            17137..17415
                     /locus_tag="PK134_00680"
     CDS             17137..17415
                     /locus_tag="PK134_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37400.1"
                     /translation="MKKTVLTLAVVAATYCANAQTEKKAEDQAQPVKSEQATTPQKEV
                     KKDAPATIQAAPANHESAPADKEVKKDAPQKDSHKATEMEKPKDKPKE"
     gene            complement(17461..17946)
                     /locus_tag="PK134_00685"
     CDS             complement(17461..17946)
                     /locus_tag="PK134_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37401.1"
                     /translation="MTQRTIHFEEKQYLGLNHYSLLRSMVLALFCFVAYYVTENRENN
                     AELLFFGGVAFMVISLLSMFIKHLHTRVYDSCIELDGLWTMRKVKIDLSTIVDVQKIP
                     YSNYIMNTPVYNLHRNGSIYFYTSGRSAIKITDKDGMNYIIGTQKQEEFLRILNDLIK
                     K"
     gene            complement(17963..19630)
                     /locus_tag="PK134_00690"
     CDS             complement(17963..19630)
                     /locus_tag="PK134_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRB37402.1"
                     /translation="MNSRILNFLLLLLISTSVNAQSDTELKSNLKQHISILASDKFEG
                     REPGTKGERLSYEYITQQFKNIGLQSLGSNGYLQSFEFSAGTKNGVKNKLTIAGKNLK
                     SDSEYYVLPYSALQATAEGKSIKIGSGLLSKDLGRNDYEGLTVLKGRIAVMDYATPEG
                     NNPHSKWAEASDLRKRIEIAVSKGISGLIIYNSNDSIKNPEKNSEIKITPFAIPVVFI
                     KSNDATLLKDNQQVTIATDIEKIMHSGHNVIGYIDNRAENTIVIGAHYDHLGYGGHES
                     LYRGKPEIHNGADDNASGTSTLIELARKLNSSSLKKYNYLFIAFSGEEKGLLGSNYFV
                     KHPTIDLKKVNYMLNMDMVGRLKPEEHILLINGAGTSPSWDSLLNNIKVDSLRIKTTA
                     SGVGPSDHTSFYLEGIPVLHFFSGTHSDYHKPSDDEPLINYNGMVSIFHFMYDLIEQT
                     NNLPKLTFTKTNDSSNEDAPRFKVTLGVVPDYAFEGSGMRIDGVTEGKPAAIAGFLKG
                     DVVIQMGEEKVADMMSYMKALSKFKKGDTVKVKVQRGKDVVEKEVTF"
     gene            complement(19678..20220)
                     /locus_tag="PK134_00695"
     CDS             complement(19678..20220)
                     /locus_tag="PK134_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRB37403.1"
                     /translation="MNAAVKYTLAGIAAVILGFVSLGIIHPEIEYESRVQISAPVTKA
                     FDIFIDTSMMKHWMPGFVSFKPLEGKMLTKGSKQLLVLNLNNEQIEITETVEDYSYPK
                     FYKFHAQNTLLNSTNTIIFEGDSIHCSLTVKTKASGNNFMLRSMFVFTEPIFKQQQDS
                     TYTLLKRTIESHLDNHNINQ"
     gene            complement(20230..>20984)
                     /locus_tag="PK134_00700"
     CDS             complement(20230..>20984)
                     /locus_tag="PK134_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRB37404.1"
                     /translation="LEEDIMNLVAHNSYKRVPNYRDLTPEQERELLDNHFNRVTDTCI
                     PEEEAFRRLQKHIHQIWKDADNKGERYFPHEYMYKMLLSGVLKQYYEIDPKNSWMLAA
                     AQKNIPIVVPGWEDSTMGNIFASYVIKNEMKATTMKSGIEYMVWLSDWYRQNSGGKGV
                     GFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDSTTSYGSYSGAVPNEKI
                     TWGKLDIDTPKFIVESDASIVAPLMFAWILEW"
BASE COUNT         6458 a         3993 c         3946 g         6587 t
ORIGIN      
        1 ccgttattcc ggcatatcca agtacttgct ggtatgaaga tccttcttcc ggtgattgga
       61 taactacaaa taatagtagc tcttcaaacg atgctgatgc ccgcacaggc gctcagtttt
      121 taagagatgc ttatagtgca accaacgaat ttatctggac tcccggattt tcattaacag
      181 caggccaaag tgttgatttt tctttctggt gggcaggtga taactacagt ggatggacag
      241 gtgatgtgtt ttacaatact gcacaaacag gaacaggagc cacacaaatg ggtacttcat
      301 ttgttacttc aggtacaaca acgacaaaaa cttatgcaaa acaaacaaga actttagttg
      361 caccagcaac aggaacttat tattttgcaa taagagtaaa tgcaacaggt ggcccatggt
      421 atttgagttt tgatgatttt aatgtagaaa atacaccgag ttgtgttgct ccaacagcat
      481 tagttggtac gccaacaaca gcaacaactg ctaacatcag ttggactgcg gcaagccctg
      541 taccatcaaa cggttatgaa tatgccgtta caacaagtgc cacacctccc ggctcaggta
      601 ctgcatttgc aggtacatca acaagcgtga gtggtttaac accatctaca acttattatc
      661 ttcatgtaag aagttattgt ggtggtagta gttatagtac ttgggcttca agtgcttcat
      721 ttatgacagg tcaaatacct gctacattgc catatagcaa tagttttgga agtaatgatt
      781 ttgcctttgt aaatggctca caaaccaatc aatgggctta tggctcggct actggaaatc
      841 ctgcaaatgc aatttatgtt tctaatgata atggtacaag taatgcatac acaatttcaa
      901 gtttaagtgt agtacaagct ttcagagata ttgctatacc taacggcact accttgacaa
      961 actttagctt taattggata gccaatggag aatcttgttg tgactattta agggtttggt
     1021 tagtaccaac atcctataat ccaacagcag gtactcaaat aacagcagga gccggcagaa
     1081 ttcagatagg atcaaacatg aatgttcaaa gtgcatggca aacctatacc aataatacat
     1141 tagaccttag ctcctttgca ggtcaaacta tgcgtttggt ttttgaatgg aaaaatgatg
     1201 gaagtattgg tacacaacca ccggcagcaa ttgataatat ttctcttgca caggttgcct
     1261 catgcagtgg tacacctgca cccggaaata cacagtcaaa tttaccatct gcttgtagtg
     1321 cccaatcatt tacattatca atgtcaaaca ctattaatga tattggaatt acttatgatt
     1381 ggcaatcgtc accagacggt ataacatgga caagcaccgg aggaaccaat gctacttaca
     1441 caacaacaca aactgctgca acatactatc agtgtgtagt tacttgtaca aatagtggaa
     1501 tgtcagctac ttcaacacct gttcaggtta caatgagtcc attttataat tgctattgta
     1561 ctacaaacct tcatacctca tcgagtccat gtattactca ggttgatttt aatactatca
     1621 gcaataatac agccggggca ggatgtgctt tacctgctta ttcttatcag gcagcaacaa
     1681 caactgtttt aaaaggttca tcatatactt ttaccagaac aacaacttca acaggggtgt
     1741 ggacaggtgt ttggattgat tatgaccaaa gcggaacttt tgatgccagt gaatatacag
     1801 aggtttcaag tacttcaaca ggagcattaa caaatagtgt tacaatcact attccggtga
     1861 ctgcattaga tggaaatacc ggaatgcgta tccgtcaacg tacagttgac atgttggcta
     1921 ctgatgcttg tacccaaggt tttggttcag gtgagacaga agattataca gttgatattc
     1981 aaacaccggt aggttgctca ggaacaccat ctgcaactat tggtggtgcc cctgctaatg
     2041 tttgtgcagg cgttcctttt acacttactg caggtccaac aggtttagca ggatttactt
     2101 atcagtttca gaactctact gatggtggtt taacctggaa taatatggga attgctgatg
     2161 tttttaataa ctacattgta agcaaccaaa cagtaacaac catgtatcag gttattgtta
     2221 cctgtacaaa cagcggttta tctaatacat caactgctgt aacagtaaca caaaattcac
     2281 caacagcatg ttattgtaca ccaactacag ttaatggatt tacgtattac atcaccaact
     2341 ttaccacaac cggtggtatt accaacatta acaatagcag tgtaggtgga ggcccctatc
     2401 agaatttcag tggtagtgca tctgcatcag caaatccggg tgctacaata aattatagta
     2461 tctctgttac aagtggtact tatggacgtg ccatttggat agacttgaac gaagatggtg
     2521 ttttccaagc aacggaacaa ttagtaacca gttcaagttt tctttcatca cctttaacag
     2581 gtagcttcat tatacctaat ggaacaacag caggtactaa acgtatgcgt gtattggcta
     2641 gctggtctta cagtggtcca acagatccat gtaatatcag tggtaatggt gagtatgaag
     2701 attacagttt tgaagttgta gcacttccat catgtacagg cacacccgca gcaacaatca
     2761 gtggtccggc aaatatttgt gcaggcattg gttttaattt aacagcaggt ccaacaggcg
     2821 ttgatggttt aacttatcag tttcagaatt caacagatgg cggaataaca tggaataatc
     2881 ttggttcagc aggcattaat aatatctatg ctataaccag ccaatcggtt actacgcagt
     2941 atcaggtcat agtttactgc tcttacagtg gattaaacgc aacttctact ccaattacgg
     3001 ttactcaaaa tttaccttct gcatgttatt gtacaccaac aaccggtggt ggtttaactt
     3061 actacatcag taactttact acaaccggtg gaactactaa tattaatcaa agtagtggtg
     3121 gcagttcaac aggttatgat gatcatagcg caacagtttc aggtgaattt gaaccaggct
     3181 caactatcaa ttttacaatg acaatagccg gatcaagtaa ctttggtcac gctatttgga
     3241 ttgattttaa tgccgatggt acttttcagg caacagaaca gattgttaca tctaatagtt
     3301 atctgtactc accaattaca ggcagcttta ctattccatt aaatgcaact acaggagtta
     3361 gacgcatgcg tattttaaca agttttacac ctagtaatcc aacaaaccca tgtaccaaca
     3421 gcggacaagg tgagtacgaa gattacagca taaacattac acctcctgta tgtatatcag
     3481 cgccatcatc acctgcaaat gcaggcagtg catgtgctga tgtaattacc aatactgttc
     3541 tgttaagctg gccttcattg tccggtgcta caggatatga tgtgtatttc gatgccaaca
     3601 atcctccaac aacattagta tcagcaaatc aggggggatt atctttcagc gcagatgtat
     3661 caggtggagc acctgtatat tactggatgg tagtgccaca gatacttggt ggtggcagtt
     3721 catgtactgt atggtcattt agtttatcac catcacctac gccaattgca agctcaggtg
     3781 gtgatgtttg tttgggtaat gatatctcct taacaggaga caacatagcc atgggacagt
     3841 caagtggaaa cacttattca tggacaggac ctaatggttt taacagcaca ctacaaaatc
     3901 caactatcat caatcccggt gttctataca gtggaactta tacagttgtt gtaaccaatc
     3961 aatatggttg ttcggcaagt gagagcacaa gtctgagtgt taatgataat ccaacattaa
     4021 gcatagactc actgaagaat gtaggatgta ttggtggcag tgatggtatt atttacgtat
     4081 cagcttcggg cggtttatct ccatatagtt ttacttctga ttttataaac tttaatgtag
     4141 atggaatcat gaataatctt ccgacaggaa tgagcactat ttatgtggca gatggcaatg
     4201 cttgtcaggc gcagatttca ggttcattaa cttataccag tactgcacct cctgcacagt
     4261 cagtaaatgt tccaatttta ggaatgcctg cttatgcttg taatggtacc acagcaacca
     4321 taagtgttgc ggcagtggct aatgcaacgc aatatacatg ggatggccct gttggaacaa
     4381 cattcaatgg taatccatca ccctatattt caactacacc atcagtaact gttgtgtttg
     4441 gagcaccaaa tggatcagga taccgcatag gtgttcaggc cggtaacggt tgtggtagct
     4501 cattacgtaa gagtcagtgg ttgcgtggta tagtaagtac tcctgcagca gtaagcggag
     4561 caactacaac atgtgcaaac agctcaggta cgtacagtac agttgctgtt gacggagcaa
     4621 catcctatct atggacagtt tcaggcgatg caactgtaag tggtaatggc acaagtgtaa
     4681 cagtaaactt tggtccttca tggacaggtg gtactctttg tgtaagtgcg cagacaccat
     4741 gtttcaattc accatcgaag tgtcttgtaa ttggctcatc agcatcacca ttgaatgtta
     4801 tcagcggatc atttacggca tgtcctaacc agacattgac attcagtgtt cctgcatcaa
     4861 caggtgcagc aagttacaat tggacgctac cagcaggtgc aacaggtagt tcaacaacta
     4921 acagcatcaa tgctacattt agtccttcat acaacagtgt aggaaacatt tgtgttagtg
     4981 taacgagcat ttgcggtgtt acctcaccaa taaaatgtaa aactgtagca cccggtcttc
     5041 catctgttcc tcctgccatc accggtatta ccaatggtgt ttgcggacag agcgttagct
     5101 acagcgtagc tccggtttca ggaattacat acaactggac agctcccgga actgttatcg
     5161 gaaatggtaa cagcacagta agtgtacagt atggctcact gacaacagga caggtttgtg
     5221 taacagcaag caacagttgt ggtaccagtg catcacgttg tgtttctgta aaaggaacac
     5281 caaactcacc gattgcatta actgcagtac cttcaagctg gtgtgccaat acacaaggta
     5341 tagagtttac tgctgatatg agtaatacaa caggatcata tacaatttcg tggacatatc
     5401 catcagcacc aacagcaagt tatgttgcag gcggaggaaa ctctaatgca cttactttgg
     5461 attggggaac aggaaacggt tcagtagttg tgactgcaac taacagttgt ggtgcaggtt
     5521 caaaagcctt tgcagtaaca ataggttgta aagaaggcga aatggcttct gcaagcaaac
     5581 tgaatgtata tccaaaccca acagcaggtg tgttgaatgt agaatacaca gctgacaaag
     5641 gcacagcaca ggtaacagtg cttgatctga gtggccgtgt agtaatgaca caatcacatg
     5701 ctaatgcagc aggacaaaac atattgcaac ttgacctgag caaagtagct aaaggagcgt
     5761 acatgctgaa tgtacaaact gaaggcagca acaatcaggt tagagtagtg gttgaataat
     5821 ttaaaactta caacttcaaa gaggttgtag gaagtaaaaa gcgggaggag caaattgctc
     5881 ctcccgcttt ttttatataa tgttgaaaca aaaattttac tgccttttag ctgccagata
     5941 taaatagggt tcataaaaat gatgaacaaa tattttggtt ttgctatccg gaaataaatc
     6001 aaagtaattt ttgtcggcaa gaagaactat ggtcgttcct gtacgaacat aatcgctgct
     6061 gttgagataa ataggcgatt gaaaatttac taagtttttc cgaactaatt ttcctgcaaa
     6121 accacctaaa tattttctgt attgcctcat ggccttatat ggtgtttttt gaagacggtt
     6181 aaatatgtgc ataacaaata agttggccgg aaatttttgt tttctgatac cagcttttgc
     6241 atttaccaaa ggattggtat tgttaatgtc ttttgtagtt tgaatggtta ttggtgtttc
     6301 cggaacccaa tcagcagaat ttacaacatt aaaagcccat ccattgcggg tcatgttttc
     6361 atattcataa gcgaaatata aatttccggg ttttggtgct gcactgcaat aggttttaaa
     6421 tcttatgtct gaagctattt ggttgttttt ttgatagtca taaagacaag aagtaactaa
     6481 aaatgctatg gcgccaccct gactatgtcc actgacaata aaattggtaa tacctttttt
     6541 ataacatgag tcaatgtgtg gcagtatatc gcgtataagg tatgcagtgg ccaatgtcca
     6601 acctgtatgc actgccgctt tagaatcatc agcaaaatga taattaaacg tatcaccggg
     6661 agctaaaatc agctgacctt tagcgggtat catacctgca taaaagtttg ccagccagct
     6721 tgtgctgttt gctattgttc ctctaatact gattactgca acagagtcag cacccatcca
     6781 caacgaccat tgattcagta agcccatctc cggtgattta tatacttctt taaagttgtg
     6841 ataatgcatt gagtcaggat atagtgagtc ctgcttatgg aattgcataa gctgaaaact
     6901 caacaactcc atgtattctt ttggattata accgggttgt agttttgatg cagtcagact
     6961 gtttacagta aacagactaa taataaaagt agttgttaaa tttctccaca taaataaaat
     7021 atttggagca aaagtaatgc aacttcagaa aaaagaattt tatgaactac tataggagta
     7081 ttctatgtat ttcttttaat gaaaaatact ttgaaggttc acttatttaa gttgcttcaa
     7141 ttttccgtca gcaaaagaaa tgattgttcc atctattgtt gataatgcaa cgttttgcag
     7201 acttactacc ttgtcaggaa atgcagtttc tgtaacttta atatttacca acgaatcggt
     7261 agttggtatt tcactataaa caaaaattga aatatctttt cttaaaccac gcggagaaaa
     7321 ttcttttttg agaagtatat ttccatcact gcctgccacc tcaaccataa tcgggcttcg
     7381 cctttttaca ataggtttta gtgactgcat ttgtcgttgt tgattggtgt tttgttcttg
     7441 aaattctgtt ggtgatgata tgtatttaaa attcaatact agcaattttt catctttatc
     7501 aaagaatgag agttttgtat tgctgaaaaa aggcattaat agaaaaaaag cacaaagtat
     7561 caatgctgct gcaatatgat tggttttaaa ataatccaac acttttatgg ttgcttttcc
     7621 atcattgggt tttgcagatg caagcgagtc ggtaaattct cttattgctt ttgcagaata
     7681 gtgatttgta gtgatgagtt gaattctgtc gcgtgataat ttttttgcta aacccggtgg
     7741 acgttttctg tttattctgg caacagccag atttctgcca tagcgatagt agcaatcttc
     7801 gcagcctaac agtaaaactc cttctgattc ttcgtttaac atttgttcaa cttcatgcat
     7861 gtgtaaatct cccaggcaag gcagcgactt ctttttatgc gtaattccat caggaatttt
     7921 tacatcatca aactgcgagc atgaaacaat actcagttga cttttctgtg ccagatttag
     7981 tggcaataca ttgtgttcag gaatgcccgg cattgttatg gcatgctctt tacaagaagc
     8041 aagacaaatt ccgcaactga tacatttgtc aggatctaaa attgccttac gttcaccttt
     8101 ataaatgagc atatcaatgg caccatatgg gcaatcttca gaacaaatat tacaggcatc
     8161 acatttatct aaatcaattg tggcaggttt gtttttcttt ttaaatataa gtggaaccaa
     8221 tgctaatccg gctccggaaa ccagcataat tacccagttc ataggaacag aaacaacttt
     8281 cataaatgag taaccgaaga agtagtacca atcgaatgtt gtattgtatg gaataaatcg
     8341 ctcttgtgct gaagggtcac tcttaaccgg aaaaaccaaa caaactaaaa ccaatgcaat
     8401 catgctataa atccagatga tgcgtggtga aaataatttt ggttttgcaa tacgcattac
     8461 gtgcagccat agaacgatta ccaggaaaat tgaaaagaaa atatgtccga atactgaaac
     8521 tttaaaaaac ccaccaataa catcaagatt gtttgaaata aatgaacctg caattgaagg
     8581 gtcgaagagt ggaattgcag aaaagaattt tgcagtaagg tagccgacta atttcccttt
     8641 ttcgtcccat acaagaataa aacctgttac accaataatg agcactacca tcaaagaaat
     8701 aattccactt acccaagaaa gaatacgttt gaattttcct gtcaataagg tttgaagaaa
     8761 atggagtaca ataaaaataa ccaataagtc actgctgtaa cgatgaatac ttctgatgac
     8821 agcattaaac ggattggcag aaattttttc tactgattcg taggcgtgag caggatcaat
     8881 attataaaac aaaaaaagat aaactccact gatgcatgca acaataaaca tcagtattgc
     8941 aatgtcgcca aggtggtata atgggtttaa cttagaagaa taaattcggc tgaagatgcc
     9001 ggcaaatcgt tgaaaaagcc aatggcttga tttattatta atcataattc tgcactgtct
     9061 tcagtgttat tggagtataa acttttaatg agaaaatagc ctacaataat gatgaacatc
     9121 attccgacag aagtttgcga aataaacttc cagtcgaggt gataagattt tgttgcaggg
     9181 ttaaaggtaa aacagcgtat gtaaagtgtt ttaaaaaaat tgttgaaaga agttttttca
     9241 acttgtgctt ccatgatggc aagttcaagg tcaatagctt tgggctcaac gccatagatg
     9301 tatcttgaaa ttcgaccact gggtgtaatg acaacaacta agtttggatg taaatactcc
     9361 ttactttttt catcatattc ataatgataa tcaattgacc ttaatagttc gccaatgcct
     9421 gctgcgttgg aggtgaccgt tttccagtta acaccatcca ttttccagcg tgcttcaaat
     9481 ccactcaaat cttctttggt atcagttgag tcaaacgaaa atgaaatgac tgtaaaatcc
     9541 tttcctaaag tacctaattt ttcgatggag gtttttaatc cgttgctgat aagcgaacaa
     9601 gccattgggc aacgggtgta aaccggtgac aacacaatgg gtttgcctgt gggataatca
     9661 aaaagctgaa ggttgtttcc ctttgaatca agaataggaa tattctttac ctcgcgacca
     9721 ataaattttt tctcatcggg aatatcgaaa gaggataaag aaaaaaacag gcttagtaat
     9781 atcactaagc ctgtaagttg cattttatta ttaatcacga accaataatt agaaaagaga
     9841 taaataaatt atttcaccca ccatgttgcc gcaagccatt tccagttaac aaagaacaat
     9901 actgcaaatg tggcaaggaa taagagtgtg agaattaatg ttccgggtgc ttcataacct
     9961 ttatgttctt tagcggcagg agccattggc gaaagtggta gctgcatgtc ttcaggcccg
    10021 tttacttttg gtccgaagaa tacggaaaca acaacctgca aaacccagat gagagctgcc
    10081 aagaaggcta aagtaccacc aagcatcatt aaagtccaga agaaatctac tgatggatta
    10141 aactcatgag tgaaaggacc tccgctgaat gttgaatccc aatgtctgcg tggaacacct
    10201 aactgaccta aggcaatcat tgatacagat aggatggcaa cgccaaagaa aaacatccac
    10261 ggttgcaatt gtgccagttt gaatgcaaca attttccttc tgaaaataag aggaatgagg
    10321 tagtaggtga tacccataaa agtgagtgta gtaccaataa ctacagtgcc tttaaagtga
    10381 ccgggaattg caatggtgtt gtgaacaata atgtttgttt gttccatacc gaaaattaca
    10441 ccggttgtac cgccaataaa tccaaaacca atacaggcca gaacacatgc tgcaaaagcc
    10501 gggtttgacc atggtgcttt agaaagccat tcaaacagcc ctttgttgaa cccccatctg
    10561 cgttgtgcgg attcaataga acttggaacg gcaaaagcat gaatcattga tgctaacaca
    10621 gcaagataca ttgcataaga agtgttccat actttccatg ctgatgatgt tgcaggatct
    10681 gttaacaagt gatgtgcgga tgcaagactg ataaagagaa tgtagaggaa gaatgctgtt
    10741 cgtgaaactc tttcattgat tgatgttccg ccaagcgcaa ggaaggtaac taaataccag
    10801 atgctgacca tggcagccac attgatctgc tgcgaagagt gaccaagtcc ccaccaaatg
    10861 agtttgtaaa cggcaggatc tacggagtca attaatccta aagaccaaag aaaggtcgga
    10921 atgtaaatga tagctcctga tgctaaggta ataacagcaa gaattgcagc tgtcattaaa
    10981 ccataagtaa agaggggtaa agtttcacca acatttccat ctctttttgc attaacaata
    11041 ttggcaaaaa ataaaccaac acctattaaa gcaccaacgg caaatagaat tataccaagg
    11101 taatagagtg gatgtgcttt tagtgggaca taagaggtta acattacatc tgcttttccc
    11161 atcaatacca ttacgttgga aagaaccata ccgcctacca tgagtaaaaa agttacccat
    11221 cctaatgttg gtgagcagaa tttctgattc aatactactg tggatccgaa atacaaacct
    11281 gcaatttcga aaaatacaat ccaataaata agagcattca aaccatgaaa ggttgttaat
    11341 ctgtagtacc attcagcatc taaaagatgt acaggttggt atcgtgtaaa cacaagcaat
    11401 atagcagctg taattgctaa gagcaatgaa actactgcca taacagcatt taatctgata
    11461 aatttttccg attgcagatc tactttaaat ccggttatat cgcatattct gaacataagc
    11521 tttagatttt atttaattga tttaatgtag gagattactt gtttaatttc attgtctgta
    11581 aggttcattt gagccattgg tgttttttca aatcctacaa caatttgttt ttgtggctct
    11641 ctgatagatg tagtaatata atcatcatca ataacaattt cttttgcatt gccatcttct
    11701 tttactttaa ccttgcttcc ataaatgctg ttgaaagcag gaccaatttc ggaagtgcca
    11761 tctgttttgt gacaagccat gcagcctttc agattataca ccatctcacc acctttaata
    11821 agttgttctt cgctccatcc ggaaatgtct tcttcagcag ttcctttgcc tgtgcgtgcc
    11881 ggtggtgcac tcaggttgtt gtaaccgtat tttgccagat cagcatcatt ctcaattaca
    11941 agaatttttc caatcatggt gtggtgacct atgccgcaga attcattaca caatactttg
    12001 gcttcaccgg caacggttgg tttaaatttc aagacataat cataatcagg atatacctga
    12061 aagttcatgt ttgaaggttg taatgaaaaa ccgtgtacta aatcttttga agaaatgtga
    12121 atgttatacc actcatcttt ctttaaaata agtaccggtg accatcgcca catttgtgcc
    12181 ataagaatga catcgctgcc gggctccggt tttacaatag gtatcccatt gtccatgccc
    12241 accatgtttc tttttacata ctcatcatgc agttgggcaa attcaatagg gttagtttta
    12301 tatgtggtgt ttgatggatt ctgacgaccg aaaacgtgcc acagtaccat ccatgcaaaa
    12361 agaaaaatac aaaccacaaa cgatatgatt atccacatgc gttcgtcttt tgaaactttc
    12421 tttttaaacc acccttcagt aggtgctatt aatgtcataa atagattgtt ttagaatttt
    12481 acttaagtat agattgttta actgattcag gaatattagg cactgtggca atctccatta
    12541 ttccccagat gatgtaaaag attgcataga ttgttactcc aagaaaaaga agtaacatga
    12601 catcatccat gactacctga agtccggaag ccttcttttt attctcttgt tcttccatat
    12661 aatttttggt ttttaattga ttattaatcg aaattttact tttcgatgac aagattattt
    12721 cagtaaagca tttttaagta tgacttgtgt cataaaagaa aatgattgtt gaaagtttaa
    12781 acatcaaagt agttagctaa accattcttg ttcttttatt tagtaagatt attttttgag
    12841 aataactttt cagtttcatt atactgaaca cgtagatttt ttaaagacag tatgtagtca
    12901 attgctttat aaaattcaaa cgaatagaat tgttgaagaa agaagtaaag ttccaggcaa
    12961 ataaaaagag gttagctttt tataaagcta acctcttttt atttgagttt acaatatgtt
    13021 tatggaatca ggaatttata cctgtcaata tttttaagtg caattcctgt accgcgaacg
    13081 actgcacgaa gtgggtcttc ggcaacgtgt acgggaagtt ttgttttcat tccaatacgt
    13141 ttatccaatc cgcgcagcaa agcaccacca cctgtaaggt aaattcctgt acggtaaata
    13201 tctgctgata attccggagg tgtcatttca agtgctttta aaatggcttc ttcaattttg
    13261 ctgatacttt tatcaagtgc atgtgcaatt tctgagtagg taactgtaat ttcttttgga
    13321 ataccggtca tcagatcacg accatgaact gcgtagtcgt gtggagggtt gtctaactca
    13381 ggcaatgcag agcctacttc aattttaatc atttcagcac tacgctcacc aataagtata
    13441 ttgtgttgtc tgcgcatata atcccaaatg tccatcgtaa aaccatcacc ggcaacacga
    13501 atagattggt cgcaaacaat tcctccaaga gcaataactg caatttcact ggtgccacca
    13561 ccaatgtcaa taatcatatt tcccattggc tcttctacat caatgccaat accaatggct
    13621 gctgccatgg gttcgtgaat aagataaact tcttttgctc cggcatgttc tgcactgtca
    13681 cgcactgctc gtttctctac ttctgtaatt cccgatggaa tacagataac cattttaagc
    13741 gatggctgaa aaaatcttct cccggggttg atcattttaa tcattccgcg aatcatattt
    13801 tctgcagcat caaaatcggc aattacacca tcttttaatg gacggatggt tttaatattt
    13861 tcatgtgttt ttccatgcat catcatagcc tgcttaccaa cagcaagtac cttacctgtc
    13921 atacggtcaa tagcaacaat acttggctca tcaaccacaa ctttatcgtt atgaatgatt
    13981 agcgtgttag ccgtgccaag gtctatggct atctcctggg tcagaaaatc aaatagtccc
    14041 atttttcttt tattgttagt gttttccttt atagtttctt cgacaatgag aacttcaggc
    14101 gtaggctttt ttttcatttt ttctgttgtt gcagacattt ttaacacttt gtctgccaac
    14161 agacttaatg tttgaaatgc cttttacctg ttaataccat agtcatgtta tttttgttac
    14221 aatattcaat agaatcttta tctttaatac ttccacccgg ctgtacaaca gcttcaatac
    14281 cagcagtttt ggctatctct acacaatccg gaaacggaaa gaatgcatca cttgccataa
    14341 cactgttttt caggttaaat ccaaaattta ctgcctttgc aatggcttgc ttcaatgcat
    14401 caacacgaga ggtttgcccg gcaccacttc caatcaactg cttattctta accaaggtaa
    14461 tagcattgct ttttaaatta cggataactc tcacagcaaa aagcatatcc tctgtctctg
    14521 tagttgttgg ctttcgcaaa gtaatgttat tccatccatc tgtgctcaat gcttcactgt
    14581 ctgtttcctg cattaaatat ccgcctaaag cagacttaat actttgcttg gcaacaagct
    14641 gtttttttaa ttgaagtata attctgtttt tcttttctga aaaaactttt actgcttctt
    14701 ctgcaaacga tggtgcaatt ataacttcaa agaaaatagc attgatttct ttagcgagtg
    14761 attcatctat atgctgatta aaaacaatta ttccaccaaa tgcagaaaca ggatcacctg
    14821 ctaatgctgc ctgccatgca tctgttgctt tttctcttgt agcaattccg caggcattgt
    14881 tgtgtttgat aattgctgca gtcacacctt tttcattgct gaattctgca ataagattaa
    14941 ttgctgcatc gcaatcaagc atgttgttat aggaaagttc cttgcctcct aattgtacaa
    15001 atgtttcatt caggttaccg aaataataac cacgctgatg tggattttcg ccataacgca
    15061 aggtaactga attgttgcat tgtaaattca aaaccggctc tgattgttca ttcttactat
    15121 agaaataatt aaaaatggca gagtcgtaat gactggttat ggcaaatgct ttttgtgcaa
    15181 gtttttttct gaatgataaa ttaacttcac cattgccctg attgtaagtt tgcagaaatg
    15241 ctgttacatc atctgcagag gcaatgatag atacatcatt aaaatttttt gctgcggcac
    15301 gaattagtga tacaccacca atgtcaattt tctcaatgat ggcactttca tcagcaccgg
    15361 atgcaactgt ggcttcaaaa ggatataaat caactacaac caaatctatg catgggatgt
    15421 tgtatttttc aacttccatc acatcatttg catcgtttct gcgatttaat attccaccaa
    15481 aaattttagg gtgtaatgtt tttactcttc caccgaatat ggatggataa tctgtcacat
    15541 cttcgacagc agtaatagga attttcaatg attgtataaa atcaaaagtt cctccagttg
    15601 caaacaaagt tacctgttgt tgatgcagag ccttgataag tggttcaaag ccatttttat
    15661 tatagacaga aataagcgca cttttaatct tcacttccgg gaattcttta aattgatgga
    15721 gagtttgcaa aaataggtaa gtgaatcaat taaaatttgt taaactgaaa ataaattcag
    15781 agcaaatcat ggcaacttta tacttttgtt gcaatgaacc gaataaggat ttttctgaaa
    15841 ttgtcagctg aaagcatagc atttgcactg catgctttga gggcaaatat gctcagaaca
    15901 gtgttatctc tattgggaat ctccataggt atttttgcca ttatcagtgt ttttacggtg
    15961 gttgatgcca tggaaaaaaa cgtacgcacc agtgtagagt cgttgggaga caagactatt
    16021 tatgttcaaa aatggccttg ggcttttgga ggcgattacc cttggtggaa atattggcag
    16081 cgtccgttac cttctgtaaa agaaatgcat cagttggctg ctcgctctca gagtattgag
    16141 gcttttactt tcgaagcata tttgaatggt aaaacagtta agtacctgaa caatagtgca
    16201 gaaaatgtaa ctgtagccct agtgtcgcat gattttgaca aggtaaaatc tttcgaactt
    16261 tcagccggtc gttatcttac tgagatggag tctaactccg gtagggctgt tggcattata
    16321 ggcagtaatg ttgctaacgg gctttttgga agtgcaagta atgctttagg gaaagttttt
    16381 gaagttatgg gtaggaaagt tgaagtgatt ggtgttttca aagaagaagg taaaagtctg
    16441 cttgaaacag agatggataa tacggtggtt ctgccattaa atttcggacg taatttcgtt
    16501 aatattcgca gcgacagaat agatcctttc atcaaagcaa aggtcaaaga aggtgttccc
    16561 attcagtttg ctaatgatga attaaaatcc attatgcgaa gcataagaag actacgccct
    16621 attgaggacg ataattttgc actcaatcag tcaagtttgt tgagcaataa catcaccaat
    16681 attttcagcg cgctgaatat ggcaggagct attatcggag gcttttctat tttggtgggt
    16741 ggctttggta ttgcaaacat tatgtttgtt tcagtgaagg aacgtaccag tcagataggt
    16801 attcagaaat ctcttggtgc caagaattat tttattctga tgcagttttt gtttgaagct
    16861 attgtacttt gtctgatagg agggttgata ggcttgagca ttatttattt tttagccatg
    16921 tttgtgtccc atgcctttga ttatccacta agccttacag gacataatat tatgcttgga
    16981 ttgtttattt cagctttaat agggttggtt tcaggattca tccctgcata ttcagcatca
    17041 cagctcgatc ctgtagaagc tatccgagcc aatggataag ttgcagattt aggatttttg
    17101 tttatgtttg ttgtaaattc tttaaatcaa ataacaatga aaaaaacagt tcttacatta
    17161 gcggtagtgg cagcaacata ttgcgccaat gcccaaacag aaaaaaaggc agaagatcag
    17221 gctcaacctg taaagagtga gcaggccaca actcctcaaa aagaagttaa gaaagatgcg
    17281 cctgcaacaa ttcaggcagc acctgccaat catgagtcag cacctgctga taaagaagta
    17341 aaaaaagatg cacctcaaaa ggattcacac aaggcaacag aaatggaaaa gccgaaagac
    17401 aaacctaaag agtaatgcag ttgtaaaaca atgtgtaaat gcccgatttt tatcgggcat
    17461 ttattttttg ataaggtcgt taagaattcg taaaaactct tcctgctttt gtgtgccgat
    17521 aatatagttc ataccatcct tgtccgtaat ttttattgca gaacggcctg atgtataaaa
    17581 ataaatactg ccatttctgt gcagattata tacaggagta ttcataatgt agttgctata
    17641 cgggattttt tgaacatcaa caattgtact gagatcaatt tttactttac gcattgtcca
    17701 gagtccatct aactcaatac atgaatcata aaccctagtg tgcaggtgtt tgataaacat
    17761 agaaagtaat gaaattacca taaacgctac accaccaaag aagagtaact ctgcattatt
    17821 ttcacggttt tctgtaacat agtaagccac aaaacaaaag agtgccaata ccatacttct
    17881 aagcagactg tagtgattca gtcccaaata ttgtttttct tcaaagtgaa tggttcgttg
    17941 tgtcatgctc tttaataatt tatcagaagg ttacttcttt ttctacaaca tcttttcctc
    18001 gttgtacttt tacttttaca gtatcacctt ttttgaattt gcttaatgct ttcatgtagc
    18061 tcatcatatc agcaactttt tcttcaccca tctgtattac cacatcgcct tttaaaaatc
    18121 cggcaattgc agcaggtttt ccttctgtaa ctccgtcaat gcgcataccg ctaccttcaa
    18181 aagcatagtc aggaacaaca cctaatgtta ctttaaaacg tggcgcatct tcattacttg
    18241 aatcattggt ttttgtaaaa gtaagctttg gtaaattgtt ggtttgttca atcaggtcgt
    18301 acataaagtg aaaaattgaa accataccat tgtaatttat caaaggctcg tcatcactgg
    18361 gtttatgata atcgctatgt gtgccactga aaaaatgcag tacaggaatt ccttccagat
    18421 aaaatgaagt gtggtcactt ggtccaactc ctgaagctgt agtttttatt ctgagagaat
    18481 ctactttaat gttgtttaat aaactatccc atgaaggaga agtgccggca ccgtttatga
    18541 gtaaaatatg ttcttcaggt ttcagtctgc caaccatatc catattcaac atataattta
    18601 cttttttcaa atcaattgtt ggatgtttta caaaatagtt tgaacccagg agtccttttt
    18661 cttctcctga aaaagcaata aacaagtagt tgtatttttt taatgatgat gaatttaatt
    18721 ttcttgccag ctcaattaat gttgatgttc cgcttgcatt atcgtcagca ccattatgta
    18781 tttcaggctt acctctgtag agtgattcat gtccgccata ccccaagtga tcataatgtg
    18841 ctccaatcac aattgtattt tctgcacggt tatcaatgta tccgattaca ttatgaccgg
    18901 agtgcattat tttttcaata tctgttgcta tagttacctg ctgattatct tttagtaatg
    18961 tggcatcatt tgattttata aatacaacag gaattgcaaa tggtgtgatt tttatttccg
    19021 agtttttttc aggatttttt attgaatcat ttgaattata aataatcaat ccactaatgc
    19081 cttttgatac ggcaatttct attctcttac gtaagtctga tgcctcagcc cacttactat
    19141 gcggattatt tccctccgga gtagcataat ccatcactgc tatcctgcct ttcaaaacag
    19201 ttaacccctc ataatcgttt ctgcccaaat cttttgataa caacccactg ccaattttta
    19261 ttgatttgcc ctctgcggtt gcctgaagtg cagaatacgg taacacataa tattcggaat
    19321 ctgattttaa attttttccg gctatggtca atttgttttt aactccattt tttgtcccgg
    19381 cagaaaactc aaatgattgt aaataaccat tacttcctaa tgattgcaaa ccaatattct
    19441 taaattgttg tgtaatgtat tcataagata aacgttcacc cttggttccc ggttcacgtc
    19501 cttcaaattt atcactggct aatatgctga tgtgttgttt taggtttgat ttcagttctg
    19561 tatctgattg cgcgtttaca gaggttgaaa tcagtaatag caagagaaag tttaaaattc
    19621 tactattcat gtctgactga taatttaatg attttttaaa ataaaattta attacaatta
    19681 ttgattgatg ttgtgattat ctaaatgtga ctcaatagtt ctttttagta acgtatatgt
    19741 gctgtcctgt tgttgtttaa agatgggttc agtaaataca aacattgaac gtaacataaa
    19801 attattacca cttgcttttg tttttacagt caaactacaa tgaattgaat cgccttcaaa
    19861 aattattgtg ttggttgaat tcagtaatgt attttgcgca tgaaatttat aaaatttcgg
    19921 atagctgtaa tcttcaaccg tttcagtaat ctcaatttgt tcgttgttga ggttcaagac
    19981 caataattgt ttagaacctt ttgtaagcat tttaccctct aatggtttaa atgaaacgaa
    20041 tcccggcatc cagtgtttca tcattgaagt atcaataaaa atatcgaatg cttttgttac
    20101 cggtgcgcta atctgcaccc tcgactcata ttcaatttca ggatgaataa tacctaagct
    20161 tacaaatcct aatataactg cggcaatgcc tgccagggtg tatttaacgg ctgcattcat
    20221 aacatattat taccactcta atatccaggc aaacatcagc ggtgcaacaa tagaagcatc
    20281 actttctaca ataaattttg gggtgtcaat atctaatttg ccccatgtaa ttttttcgtt
    20341 tggcacggca ccgctataag aaccataact tgttgtactg tcgctaatct gacagaaata
    20401 gctccagaaa ggtacatcat gccattccaa atcctgatac atcatgggta caacacaaat
    20461 cggaaagtca cctgctatac caccaccaat ctgaaaaaat ccaacgccct taccgcctga
    20521 attctgacga taccagtcgc taagccaaac catatattca ataccgcttt tcatggttgt
    20581 tgctttcatt tcatttttaa tgacgtatga tgcaaaaata tttcccatag tgctgtcttc
    20641 ccagccggga actacaattg gaatattttt ttgtgctgct gcaagcatcc atgagttttt
    20701 aggatcaatc tcatagtatt gtttcaacac accactcaaa agcattttat acatgtactc
    20761 atgaggaaaa taacgctcgc ctttattgtc ggcatctttc catatctgat gaatatgttt
    20821 ttgtaatctt ctgaaagctt cttcttcggg aatacatgtg tcagtaacac ggttaaaatg
    20881 attgtctaat aattctcgtt cttgctcggg agtaaggtcg cggtaattag gaactcgctt
    20941 ataactgttg tgtgctacaa ggttcataat atcttcttca agat
//
LOCUS       DAORQT010000008        19302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_213_length_19302_cov_12.630020, whole genome shotgun sequence.
ACCESSION   DAORQT010000008 DAORQT010000000
VERSION     DAORQT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19302
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213_length_19302_cov_12.630020"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5307)
                     /locus_tag="PK134_00705"
     CDS             complement(<1..5307)
                     /locus_tag="PK134_00705"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRB37405.1"
                     /translation="MTTKIAKTKRLFHLSRITFFLLCLFITGLAKAETEPNDSKAQAN
                     TLTLNTGMPGTVALSESDWFSVTTPEDGNLNITGTFSSYIYIQVYDNDGTTNLFLNYH
                     SGTVTRTVYGLAAGTYYVRLYNYTATETNTYTITPSLTATGFANDTEDNSTPALANVL
                     PLNGSVTGHVGYYYNNQRDTIDWYAVTVTQDGTLSYTITSENGLYIYAKLYDGDGTTQ
                     LAGNYTGSTATFSRGDMAPGVYYILIHAYYPNSEYVPYTLSNTLIPPVSANDPLPNET
                     FSTATPLALNGSTTGHIGYRYNGNVDVYDWYSVNTNADGDLYFTITSDNGQYVYAELF
                     DGDGITNLAGNFTTSTATYHKDGLAPGTYYIRIRTFYSYEYTSYQLSDSLGLPIYAND
                     ATPNETYSAATVLPLNDTITGHIGYRYNGNIDAVDWYSVTTNADGRLYWTITVDNGQN
                     VYAELFDGDGTTNLGGSYTTTTATFNANGLAPGMYYIRIKTFYAYQFASYQLSNSLVE
                     PIQANDALPNDIYTTATPLTLNDSTTGHIGYRYNGSVDVYDWYAVTTNADGQLFWTIT
                     SHNGQYVYAHLFDGDGITLLAGNYTSTTATFNENGLAAGTYYIRIKTYYGYEFAPYTL
                     SNSLTPALYTNDTEPNGSVATAVPLAVNTTVTGHSGYYYNNLDDTLDFYAITLPVDGK
                     LTWTITSGNGQYVYAQLYDGDGTTYLGGSYTNSTATYTKNNLAAGTYYIRIKTYYNNE
                     FTPYTLTNVLDPMNFAAENSADNAYAISPTLLPANTATTGHINFYYYGVNDTRDWWVM
                     GYDGTGAMTITCELEQNHFDVNYPDIAYAVYTDTAAAPIYSGTWINPTNTLTIPSPPI
                     SKYYLRLTQSANTFGAYRLTAAYTENCANNITSTSSQIVPCLGTINYTVSGGLADYTV
                     QLYKDGNPFGAPQTTSSTVSFTNLGIGTYYLQSHSFGASASCNNVSGNTVFSAPPVPN
                     ITPGGPTTFCAGGNVVLTADAASSYLWSTGATTQAITVSASGNYVVTVYNAALCSNTS
                     ASTSVTVNPLPPTPTITPNGPLTFCANSNVTLSSSVATGYLWSNASTTQSIVVNTAGT
                     FTVTINDANGCTASSASVTTNTIPLQTYYRDLDGDTYGDLGNTIQDCSPPAGYVSNST
                     DCNDYDIALHPGATELCNGIDDNCDSQVDEGVLSTFYADADGDGYGDLTVTTQACSAP
                     IGYVSNSTDCDDNNNAVHPGATEICNGIDDNCDGQIDEGCGSYTWYADTDGDTYGDPG
                     NFIVNYNPTPPAGYVADNTDCNDANNSIHPGATEIACNGIDENCNGMADDLGTPSTDP
                     SSITSNAVFDEICLGGNAILTVNGGSLGTGASWQWYSGSCGGTPVGTGSSITVSPSSA
                     TNYFVRAEGACGNSACVNLTITVKTSLPAHAVVIPPISGLPAYACPGSSASISIPAVP
                     NASQYIWDGPTGTYFNGNPLNTSPFTSATPNVQITFGTTNSSFYNIGVQAANSCGATL
                     RKIQKVRYKISTPASISGAVNACANSSSTYSIVAVTDATQYLWSITGDASVVGNGTTV
                     TVNFGPAWAGGNLCVAAQTPCFTSPAKCMYISSSATALNAISGTFTACPGSVITYSVP
                     PSAGASYYTWTLPAGASGSSNTNSIVVTFGPSYNTVDDICVTVTSVCGVTSAPKCKTV
                     APGLPARPNAITGPSNGLCAQTAPYSVSSSLGITYNWTAPGSISGNGTSAITVSYGSF
                     TTGSVCVTASNACGTSAARCITIKGAPNTPVSLTATPATWCAN"
     gene            5695..5838
                     /locus_tag="PK134_00710"
     CDS             5695..5838
                     /locus_tag="PK134_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB37406.1"
                     /translation="MGFKAEITDISTLTVRKHIANIYNKLHVNSKAQVISMAHKNKWF
                     NIF"
     gene            complement(5841..6470)
                     /locus_tag="PK134_00715"
     CDS             complement(5841..6470)
                     /locus_tag="PK134_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRB37407.1"
                     /translation="MTTRNHQVYAELTPNPNSVKFVADRLLLFEGTVEYKSKAEAVDC
                     PIAYQLFDFTGIKSVFITTNFITITKEADIDWYEITNILREFIKGFLNSGEKLFLKNP
                     FPEQAETKEVIASKAEELVKQPVTQNPTEHSELEEKIITMLDEYVRPAVEGDGGAIDF
                     KSFKDGVVTVVLKGSCSGCPSSTLTLKAGIENLLKRMIPEVSEVMAEAE"
     gene            6547..8103
                     /gene="nadB"
                     /locus_tag="PK134_00720"
     CDS             6547..8103
                     /gene="nadB"
                     /locus_tag="PK134_00720"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRB37408.1"
                     /translation="MTKTDFLVIGSGIAGLSFALKAAKHGKVTIITKANEDESNTKYA
                     QGGIAAVLPEAKDDSLEHHVQDTLSCGAGLCDEKIVRMVVAESGERINELIAWGTEFD
                     KNKSGNFDLAMEGGHSAHRVLHHKDITGLEIETKLLQQIHQHPNITLHTHLFAVDIIT
                     QHHMGVIVKRNTPGIACYGAYILNTKTGKVETMLAKVTVMATGGAGHVYKTTTNPVIA
                     TGDGIAMTYRAMGKVNGMEFIQFHPTALYHPGTYPSFLISEAVRGFGAVLRTIDGKEF
                     MQRYDERGSLAPRDIVARAIDSEMKKSGDDYVWLDCTKLNITEFKKHFPNIYNRCMSL
                     GIDVAKDCIPVAPAQHYLCGGIKVDEHGQSSIQHLYAIGECSSTGLHGGNRLASNSLL
                     EAVVFAHRAYEHASERLEFIHYNEHIPEWNSVSPREAAEMVLITQSRKELQEIMSSYV
                     GIFRSDMRLKRALERTRLLYEETEDLYNRWEISPRICELRNLINVAYLIIKAAMARTE
                     NKGLHFNVDN"
     gene            8119..9084
                     /locus_tag="PK134_00725"
     CDS             8119..9084
                     /locus_tag="PK134_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRB37409.1"
                     /translation="MISIFENNFNFENTALESFRFQAENNAVYRNYLKLLNIKPNQIS
                     NVSQIPFLPISFFKTKKVVSGNNDSKAIVFSSSRTTGAEPSLHYVNDISLYEKAFTQT
                     FNQYYSSADQWCILALLPSYLEREGSSLVYMCQHLIERTGKKNSGFYLNDFRKLADTI
                     IVNEQKGIKTLLIGVSFALLDFAHTHPMPLKNTIIMETGGMKGRRKEMIRQELHTILM
                     EAFQLNAIHSEYGMTELLSQAYSSGNGIFKCPPWMKVFVRDSNDPLSESKPGQGGINI
                     IDLANINSCSFIATQDIGRVHADGTFEILGRFDNAEVRGCNLLIE"
     gene            9098..9445
                     /locus_tag="PK134_00730"
     CDS             9098..9445
                     /locus_tag="PK134_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRB37410.1"
                     /translation="MEKVTNLDFIKGFSKGDMSRVKKYVMMYIQSADEEMPVIEKSFN
                     DKDYKKLKTSSHTLKSIAGYMGMDHAQQLLKKIEDSASNESVTEEMSHDIAQLKEMIT
                     LSKDELKAFIAQN"
     gene            9482..10000
                     /locus_tag="PK134_00735"
     CDS             9482..10000
                     /locus_tag="PK134_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB37411.1"
                     /translation="MSKSSTIFIIDDDPMMQSMLKDHLSAMSNFTIHTFATGEEALAS
                     GQLVPDIVFLDYNLDSKIANAMDGIDVLREIKSKYPATEVVMLSGQDKIEVAVDTMKY
                     GAFDYIVKGESAFYRAEKTVFNLFKLAQLKKNAGMYKILTITFGAALLLMIILVIILK
                     AKGMITDHVGWY"
     gene            10013..10498
                     /locus_tag="PK134_00740"
     CDS             10013..10498
                     /locus_tag="PK134_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37412.1"
                     /translation="MKTVLALLLIFSLTSCRKEEKYPIEPVLLYKSVQFYEKNSFYLT
                     TTFTDGDGDIGLSDADTLPPYDQTSHFYHNYFCNYQQLINGSWTPINFSIPYYYRIPM
                     LTPKQRNKNIKGDIQVDVKNFLSFTTNGIADTFRVEISVADRALHESNKVLSDAFVIY
                     K"
     gene            complement(10540..13308)
                     /gene="leuS"
                     /locus_tag="PK134_00745"
     CDS             complement(10540..13308)
                     /gene="leuS"
                     /locus_tag="PK134_00745"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRB37413.1"
                     /translation="MDYQFRETEKKWQQYWAANKTFKVENKSDKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGYDSFGLPAEQYAIQTGQHPAITTET
                     NINRYREQLDKMGFSFDWDREVRTSDPSYYKWTQWIFLKLFDAWYNNKSNRAESIDKL
                     ISEFEKNGNNDISAVHDEIENFTAAQWKNFDETEKSRILMAYRIAYLGEAYVNWCPAL
                     GTVLANDEVKDGLSERGGHPVERKLMKQWSMRITAYAERLLQGLENIDWSDSLKEAQR
                     NWIGKSEGTTIRFSLQNSDDYIEVFTTRPDTLFGVSFVTLAPEHELVDKITTSSQRAE
                     VQDYVTKTKNRSERERQAEVTRISGVFTGSYVIHPFTGNKIPVWIGDYVLAGYGTGAV
                     MAVPAHDERDFRFATHLGLDRPQVIEPNITHDFGKGSWDAKEGKLINSGFLNDLDVKS
                     AISCCITEIEKQNLGKGKTNYRLRDAVFGRQRYWGEPIPIYYKENIPVAIPENELPLV
                     LPEVDKFLPTETGEPPLARAKNWKYKNLFPLETTTMPGWAGSSWYFLRYMDANNNTSF
                     ASKDALNYWQNVDLYIGGSEHATGHLLYVRFWTKALKDLGYINIDEPAQKLINQGMIQ
                     GTSCMVHREVATNKIISVGLKSNYNTTLIHIDVNLVDNTEVDIEGLKKWRDDFATAEF
                     VTENGKLICETAVEKMSKRWYNVVTPDSICEKYGADCLRMYEMFLGPLEQSKPWNTNG
                     IAGVSNFIRKLWKLYHNNGKFEVSNETATKAELKTLHKTIKKVQEDVEKFSFNTSVST
                     FMICVNELTEHKCNKREVLEPLAIILSPYAPYIAEELWQLLGHSNSVSFATFPEWNET
                     YLIENEFDYPVSVNGKTRFKIQLPLSIDKEEIERHVMASEEMQKWTNGNVPKKVIVVP
                     GRIVNVVI"
     gene            complement(13367..14137)
                     /locus_tag="PK134_00750"
     CDS             complement(13367..14137)
                     /locus_tag="PK134_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB37414.1"
                     /translation="MSLDLFYRSSGNGQPLIILHGLFGLNDNWQSFSKQITENNIAVY
                     AVDLRNHGSSPHDSVFDYDSMADDVFAFISKHDLQNVNIMGHSLGGKVAMKFALKHQD
                     ILKSLIVVDIAPRYYAPHHQQIIKALQAVDLDTVNTRKEAEHILSGYIDDAGVKMFLL
                     KNLYWEGDRLQWRFNLKVIAEKIESVGERITSTEKFEGKTLFIKGEKSKYITDDDKSE
                     ILNLFPEAEIKTCANAGHWVHADNPECMLTTVRDFLSQ"
     gene            complement(14137..16578)
                     /gene="pheT"
                     /locus_tag="PK134_00755"
     CDS             complement(14137..16578)
                     /gene="pheT"
                     /locus_tag="PK134_00755"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRB37415.1"
                     /translation="MKISLDWLKDYIKLQQTPEEIEHLLTSSGLEVESLDETDSIKGG
                     LRGLVVGEVLEKEKHPDADRLSVTKVNVGKTIPLQIVCGAANVAKGQKVLVALNGARL
                     YPTFGEPFDIKNSKIRGQASEGMICSEDEIGLGNSHEGIMVLDANAVPGTPAAEWFKI
                     KQDHIFEIGLTPNRIDAASHGGVARDLAAVINTRAKAENNNSTTAQFSMPDVGAFKSE
                     NNALRIDVVVDKNSGCGRYCGVSISGVTVGESPEWLKSRLQSIGVKCINNIVDVTNFV
                     MFETGQPLHAFDAARVKGKIIVQKTGKASVFTTLDHIERKLTGDEVMICNATEPLCIA
                     GVMGGTDSGVSASTTQIFLESAWFNAAEVRKAAKHHGLKTESSFRFERGTDPNGTLYA
                     LKRAALLIKEVAGGKITSDVVDLYPEVVEPRIIYVNFEKINKLSGINFSNDMLLSVLE
                     YCGFSIKSSNVNGATISIPTYKTDVTRQADVVEEILRTYGYHHIPLNEKTGISFSSHT
                     PDRKPAMQYKISHHLSSIGFFEMINNSLVSSSHSSAGDEAVKVLNPLSKELDILRPDM
                     VASTLHTICYNINRKNHNLKLFEFGKTYLQHEGKLKETNNLVIAMTGMVNEPNWHKSE
                     KESSLFYLKSVAEKAIELVMPRISSHLNWVQDSHTLFSTFANIMQRQSAFGYIGSVKK
                     NILKQFDIAQPVYLAVLNIDVLLEKSLSHKTAIKLPQPFPEVKRDLSLMLDQAINYAD
                     LRDLALKQEKKLLKQVDIFDVYEGEKIEAGKKSYALSFTLADDEATLTDKQIESVMQK
                     IEKAFEKNLGAVIRK"
     gene            complement(16653..18890)
                     /locus_tag="PK134_00760"
     CDS             complement(16653..18890)
                     /locus_tag="PK134_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRB37416.1"
                     /translation="MGNMTNKKKRGVFRMFVLLMWVVALLPLAIIVLVLGAVNMGKFG
                     ELPTFEELENPNSSLASEVISSDNVLLGKYYIQNRSNIHYKELAENTVDALKATEDIR
                     FEEHSGVDMRGLGRVFFKTVILQQRSSGGGSTITQQLAKNLFPRENESGFRLAVRKVK
                     EWIIAVRLERNYTKQEIMAMYLNTVEFGHNSYGIKSAAKTFFNKMPSQLKVEESAMLV
                     GLLQAPTRYSPVRNPKNALIRRNTVLSQMEKYKFLSEAAYDSIVKLPIKLNFRAEDHN
                     DGLAPYFRETLRLELVKWCKEHKKSDGSSYNLYRDGLRIYTTIDSRLQEYAEEGMKEH
                     LTELQKTFYQHWKGRVPWAQHPEVIEDGIKKSDRYISLKAEGLSEQEIKENFATKIPM
                     TIFSWKGDIDTMMSPLDSLKYYKMFLQAGVMSIEPQTGYVRAWVGGNNYRYFKYDHVK
                     AGRRQVGSTFKPFLYTLAMQEGYSPCFKVPNVRVTIPIPGQPDWSPSNSDGKYGGMLT
                     LKEALAESVNSVSAYLMKQFGPKPMIEIARKMGITSPIDEVPSICLGTPDVSLFEMCG
                     AYSTFANKGVWTEPIYITRIEDKNGMVLQEFVPRKVEAISEETAYLMLNLMQGVVQSG
                     TGGRLRFRFGLTNPIAGKTGTTQNQSDGWFMGITPTLVTGVWVGCEDRSVHFRTTSLG
                     QGANTALPIWALYMKKAYADKDLNLYNGDFEKPEHPLSIETDCSKYEQDKKDHQHNSG
                     FGSDL"
     gene            complement(19112..>19302)
                     /locus_tag="PK134_00765"
     CDS             complement(19112..>19302)
                     /locus_tag="PK134_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37417.1"
                     /translation="TIQLPEDLGSDITVELYTSEGSLVSRRIINSTQLFSLPPSAGLY
                     FIRVISPQQSQTIKLVVE"
BASE COUNT         5792 a         3884 c         3665 g         5961 t
ORIGIN      
        1 gtttgcacac catgttgcag gggttgcagt taacgaaaca ggcgtattag gtgctccttt
       61 gattgtaatg caacgagcag cacttgtgcc gcaggcattg ctggcagtaa cacaaacaga
      121 gcctgtagta aaagagccgt agctaacggt aatggcactg gtgccgttgc ctgaaataga
      181 accaggtgca gtccaattgt aagtaatgcc gagcgaagaa ctaacactgt aaggagccgt
      241 ttgtgcacac agaccgttag aaggaccggt gatagcatta ggtcttgctg gtagtccggg
      301 agcaacagtt ttgcatttag gcgcagaagt aactccacaa acacttgtaa cggtaacaca
      361 tatatcatct acagtgttat aagatgggcc aaaggttaca acaatactgt tggtattaga
      421 actaccagaa gcaccggcag gaagagtcca tgtatagtaa gaagcaccag cagaaggagg
      481 cacactgtaa gtgatcacac taccgggaca tgctgtaaat gttccgctga tggcgttaag
      541 agcagtagca gaagaactga tgtacataca ttttgccgga gaagtaaagc aaggagtttg
      601 agcggcaaca caaagattgc caccagccca tgcgggacca aaattaacag tgactgttgt
      661 accattacca acaactgagg catcaccggt aatgctccac agatattgtg tagcatcagt
      721 tactgcaaca atagaatatg tgctactgct gttggcacat gcattaacag caccagagat
      781 tgaagcaggg gtactgattt tataacgtac cttttgaatt ttacgaagtg ttgcaccgca
      841 actgtttgcg gcttgtacac ctatgttata gaaggaggag tttgtggtac caaaagttat
      901 ctgcacgttt ggagttgctg aagtgaatgg tgaagtgttt aacggattac cgttaaagta
      961 ggttccggta ggtccatccc atatatactg agaagcatta ggtacagcag gaatacttat
     1021 tgaagcagaa gagccgggac atgcataagc aggtaagcct gaaataggag gaataactac
     1081 ggcatgtgca ggcagagagg tttttacagt gatagtgaga ttaacacatg cagaattacc
     1141 acaagcacct tcagcacgaa cgaaataatt agtagctgaa gaaggagaaa cagtaatact
     1201 gctacctgtt ccaacaggag tgccaccaca agagccactg taccactgcc atgaagcacc
     1261 ggtgccaaga ctaccaccat ttacagttaa aattgcatta ccacctaaac agatttcatc
     1321 gaatacagca ttgcttgtaa tggaagatgg atcagtactt ggtgtaccta aatcatcagc
     1381 catgccatta caattttcat caataccgtt gcatgcaatt tcagtagcac caggatgaat
     1441 agaattattg gcatcattac aatcggtgtt atcagcaaca taacctgcag gaggagtagg
     1501 attataattt acaataaaat tgccaggatc accataggtg tcaccatcag tatcggcata
     1561 ccatgtataa ctgccacaac cttcgtcaat ctgaccatcg cagttatcat caatgccatt
     1621 gcatatttcg gttgcaccgg gatgaacagc attattatta tcatcacaat ctgtactgtt
     1681 gctaacatat cctataggag cagaacaagc ctgagtagta actgttaaat cgccataacc
     1741 atcgccatct gcatcggcat agaaagttga aagaacacct tcgtcaacct gactatcgca
     1801 gttatcatca ataccgttac aaagttccgt agcaccaggg tgaagagcaa tatcatagtc
     1861 gttacaatcg gtgctgttag aaacatagcc tgctggtgga gaacaatctt gaatagtatt
     1921 accaagatca ccataagtat caccatcaag atcgcgatag taggtttgca gaggaatggt
     1981 gtttgttgta acagatgctg atgaggcagt acagccattg gcatcattta tagttaccgt
     2041 aaatgtgccg gcagtgttaa caacaatact ttgtgtagta gaagcattgc tccataaata
     2101 gccagtggct acacttgaag atagcgttac attgctgttt gcacagaaag taagcggacc
     2161 attaggtgta atggttggtg ttggaggcaa tgggttaaca gttacagaag ttgatgcaga
     2221 agtattgctg cacaatgctg cattgtaaac tgtaaccaca taattacctg atgcacttac
     2281 tgtaatggct tgtgtagttg caccggtact ccagagataa gatgaagcag catcggcagt
     2341 taaaacaaca tttccacctg cacagaaggt agtaggacca ccaggagtga tgtttggaac
     2401 cggaggtgca gagaatactg tgttaccact tacattattg caagaagcag aagcaccgaa
     2461 ggaatgtgat tggagataat aagttccaat accaagattt gtgaagctaa cagtagaaga
     2521 agttgtttgt ggtgcaccaa atggattacc atctttataa agctgaacag tataatctgc
     2581 taaaccacct gaaacagtat agttaatggt gccaaggcaa ggtactattt gcgaagaggt
     2641 gctggtaata ttgtttgcac aattttctgt ataagcagct gttaaacgat aagcaccaaa
     2701 ggtgtttgca gactgtgtca gtcgcaaata atatttgctt atgggtggag aaggaattgt
     2761 taatgtattt gtgggattaa tccaagtgcc ggaatatatt ggagcagcag ccgtatcagt
     2821 ataaactgca taagcaatat caggataatt aacatcaaaa tgattttgct ccaattcgca
     2881 ggtaatagtc attgctcctg tgccatcata acccatcacc caccagtcgc gtgtgtcatt
     2941 aactccgtag taatagaagt tgatgtgacc tgttgttgct gtgttggcag gtagcagtgt
     3001 tggagaaatg gcataggcat tatcagcaga attttctgct gcaaaattca tcggatccaa
     3061 tacatttgtt aatgtatatg gtgtaaactc gttgttgtaa taagttttta ttcttatata
     3121 gtatgtgcct gctgccagat tatttttagt gtatgtagca gttgaatttg tataactacc
     3181 acctaagtaa gttgtaccat caccatcata aagttgagca tacacatatt gtccgttgcc
     3241 tgaagttatt gtccaggtaa gtttaccatc tacaggaagt gtaatagcat aaaaatctaa
     3301 agtatcatct aaattgttat aatagtaacc actgtgtccg gttacagttg tattaactgc
     3361 taatgggact gccgtagcaa cactgccgtt tggttctgta tcattagtat aaagcgcagg
     3421 cgtaagtgaa ttgcttaaag tatatggtgc aaattcatat ccatagtatg ttttaattct
     3481 tatataataa gtgccggcag caagtccgtt ttcattaaaa gtggcagtag ttgatgtata
     3541 gtttccggct agcaatgtta ttccatctcc atcaaaaaga tgtgcataaa catattgtcc
     3601 gttatgcgaa gttattgtcc agaatagttg accgtctgca ttggtagtta cagcatacca
     3661 atcataaaca tcaacacttc cgttatagcg ataaccaata tgaccggtag ttgagtcatt
     3721 tagtgtaagt ggtgtagctg ttgtataaat gtcatttggc aaagcatcat ttgcctgaat
     3781 aggttcaact aaactatttg atagctgata ggatgcaaat tgataggcat agaaagtctt
     3841 aatacgaatg tagtacatgc ccggtgccag accatttgca ttgaaagtgg ctgttgtggt
     3901 cgtataactt ccacctaagt tagtagtacc atcaccatca aatagttctg cataaacatt
     3961 ttgaccgtta tcaactgtaa tagtccaata taaacgtcca tcagcattgg tagtcacact
     4021 gtaccagtca acagcatcaa tatttccgtt ataacgatat ccaatgtggc ctgtaattgt
     4081 atcgttcaag gggagtacag ttgctgctga gtaagtttca ttcggtgttg catcattagc
     4141 ataaatcggc aaacctaaac tgtcagataa ttgataagaa gtatattcat aagagtaaaa
     4201 agttctgata cgaatataat aagtgcccgg tgctaaacca tctttatgat atgttgcagt
     4261 agatgttgta aagtttccgg ctaagtttgt aatgccatca ccatcgaata attctgcgta
     4321 aacatactgg ccattatcac tggttatagt aaagtataag tctccatcag catttgtatt
     4381 aacactatac cagtcataaa cgtcaacatt tccgttgtag cgatatccaa tatgacctgt
     4441 tgtactgcca tttaaagcaa gcggtgttgc agttgaaaaa gtttcatttg gtaaggggtc
     4501 atttgcacta acaggaggta ttaatgtatt ggatagtgta tatggtacat attcactgtt
     4561 agggtagtat gcatgaatta atatataata tactccaggc gccatatcac ctctggagaa
     4621 agttgcagta cttcctgtat aatttcctgc aagttgggtt gtgccatcac catcataaag
     4681 tttagcataa atgtataaac cattttcact tgtaatagtg tagcttaaag tgccatcctg
     4741 agtaacggta acagcatacc agtctatagt atcgcgttgg ttgttgtaat agtagccaac
     4801 atgtccggta acacttccat tcagaggtaa cacatttgct aatgcaggag tgctgttatc
     4861 ctctgtgtcg ttggcaaagc ccgttgctgt caggcttggt gttatcgtat aagtgttggt
     4921 ttccgtagca gtgtaattgt atagtcgcac ataataggtg cctgctgcca agccataaac
     4981 tgttctggtt acggttccag aatgataatt gagaaagaga ttagttgtac catcattatc
     5041 gtaaacctga atataaatgt aactcgaaaa agtaccggtg atattcagat ttccatcttc
     5101 cggagtggta acagaaaacc agtcagattc actgagtgca acagtaccgg gcattcctgt
     5161 atttaatgtt aatgtgttgg cctgagcctt gctgtcatta ggctctgttt cggcttttgc
     5221 taagcctgta atgaacaagc atagcaagaa aaaggttatt cttgataagt gaaacaacct
     5281 ttttgttttt gcgattttag ttgtcataat aatttggttt ttaagtgtga aataatttat
     5341 tttctatttt gcaataatgt gactttttaa gtatgctgtc aatcgccaat tctggtgatt
     5401 tttttaattc agaaagctac gtattcttat ttgcgtcttt tgaaccgaga attattagat
     5461 tattttaatg ctacaaatat ttctactaat taattttatt gtaaatactt aatatgtagt
     5521 aatcagaaaa tatttcagat gaatgatact acaaattgag tattgacttt tttggcttaa
     5581 aaaaaagatc ctgatttcaa tctgtttatt tagttttacg gtattaatta ttaaaaaggc
     5641 attcagatgt ttttctgttt tggtgtttat tttggcacag ttgaataatg caatttgggt
     5701 tttaaagctg aaataacaga catcagcact ttaactgtta gaaaacatat tgcaaatatt
     5761 tacaacaaac tgcatgtaaa ttctaaggcg caggtaattt caatggcgca taaaaacaaa
     5821 tggtttaata ttttttagaa ctattcggct tctgccatca cttccgaaac ttcaggaatc
     5881 attcgtttca gcaaattttc aattcctgct tttaacgtta atgttgacga aggacaacca
     5941 ctgcatgagc cttttaaaac tacagtaaca acaccatctt taaacgactt aaaatctatg
     6001 gcaccgccat caccttcaac ggcaggacgc acatattcat caagcatggt gataattttt
     6061 tcttccagtt cagaatgttc agttgggttt tgtgtcaccg gttgctttac caattcttct
     6121 gcttttgagg ctattacttc ttttgtttca gcttgctccg gaaaaggatt tttaagaaat
     6181 aatttttcac cgctatttaa aaacccttta ataaactctc gcaagatatt ggtgatttca
     6241 taccaatcaa tatcggcttc ctttgttata gttataaaat ttgtagttat aaagacagac
     6301 ttaataccgg taaagtcaaa caactgataa gctatcggac aatctaccgc ttcggcttta
     6361 gatttgtatt caacagtgcc ttcaaaaagc aacaacctgt cggcaacaaa ttttacactg
     6421 ttgggattag gtgttaactc ggcatagacc tgatgatttc ttgttgtcat gaatagttac
     6481 ttttgcaatt aatttacgaa agtaaacata ttcgacttat tttagtttaa aatttcgatt
     6541 aataaaatga caaagaccga ttttctggtg ataggcagcg gaatagccgg cttaagtttc
     6601 gcattaaagg cggccaagca tggcaaagta accatcatca caaaggctaa tgaagatgaa
     6661 tctaacacca aatatgcaca aggcggcatt gcagccgttt tgccggaagc aaaggacgac
     6721 tctttagaac atcatgttca ggatacgttg agttgtggtg caggattgtg tgatgagaag
     6781 atagtgcgca tggtagtagc cgaaagtggc gaacgcataa atgaactcat tgcctgggga
     6841 acagaatttg acaagaacaa gagtggcaac tttgatttag ctatggaggg tggtcattcc
     6901 gctcatcgtg tattgcatca taaagacatt accggacttg aaattgaaac aaagctgctt
     6961 caacagatac atcagcatcc aaacatcaca ttacataccc atctttttgc tgttgacatc
     7021 attacgcaac atcacatggg tgttattgtt aaacgtaata ctccgggaat agcatgttat
     7081 ggtgcatata ttttaaatac taaaaccggc aaagtagaaa ctatgcttgc aaaagttaca
     7141 gtgatggcta ccggtggtgc aggacatgtt tataagacta caaccaatcc tgttattgca
     7201 accggtgatg gcattgccat gacctatcgt gccatgggaa aagtaaatgg catggagttt
     7261 attcaatttc atcccacagc attgtatcat cccggaacct atccatcttt tctcatttca
     7321 gaggcagtgc gtggttttgg tgcagtgttg cgaactattg atggcaaaga atttatgcaa
     7381 cgctatgatg agcgtgggtc gcttgcgcca cgcgatattg ttgcccgtgc aattgatagc
     7441 gaaatgaaaa aaagtggtga cgattatgtt tggcttgatt gtacaaaatt aaatataaca
     7501 gagtttaaaa agcatttccc gaatatttac aaccgatgca tgtcgttggg cattgatgtt
     7561 gctaaagact gtattcctgt tgcaccggca caacattatt tatgtggagg tattaaagtt
     7621 gatgagcatg gacaatcgtc tattcaacat ctttatgcaa ttggagaatg ttcgtcaacc
     7681 ggactgcatg gtggaaatcg tctggcgtca aattcattac tcgaagctgt tgtatttgcg
     7741 catcgtgctt atgagcatgc ctcagagcga ttggaattta tccactacaa tgaacacatt
     7801 ccggagtgga attcagtttc gccacgcgaa gcagcagaaa tggttcttat tacacaaagc
     7861 cgaaaagagt tgcaggaaat aatgagcagc tatgttggaa ttttcagaag tgatatgcga
     7921 ctaaaacggg cgcttgaacg tacacgactt ttatatgaag agacagaaga cctgtataac
     7981 agatgggaaa tttctcctcg tatatgcgaa ttgcgcaacc tcattaatgt agcatacctg
     8041 attattaaag cagcaatggc aaggacagaa aataaaggac tgcattttaa tgtagataat
     8101 taaattaatt gcttctgtgt gatttcaatt tttgaaaaca attttaattt tgaaaataca
     8161 gcacttgaaa gttttaggtt tcaggcagag aataacgctg tttatcgcaa ctatctgaaa
     8221 ttattaaaca tcaagccaaa tcagataagt aatgttagtc agattccttt tctgccaata
     8281 agttttttta aaacaaaaaa agtagtctca ggaaataatg actcaaaagc aatagttttt
     8341 tcaagtagca gaactacagg tgcagagcca tcactacatt atgtcaatga tatttcttta
     8401 tacgaaaagg cttttacaca aacatttaat cagtattatt cgagtgcaga ccaatggtgt
     8461 atattagctt tattgccttc ttatctggag cgcgaaggtt catcgctggt gtatatgtgt
     8521 caacatctta ttgaaagaac aggaaagaag aacagtggat tttatcttaa tgatttcaga
     8581 aaattggcag ataccattat agtaaatgaa caaaagggaa ttaagacttt acttatcggt
     8641 gtaagttttg cattgcttga ctttgcccat acacatccga tgccactaaa gaacacaatt
     8701 atcatggaaa caggaggtat gaaaggacga agaaaagaaa tgataaggca agaactgcat
     8761 actatcctga tggaggcgtt tcagttgaat gcaattcatt cagaatatgg aatgacagaa
     8821 ctgctttcgc aggcatatag tagcggtaac ggtattttta aatgcccgcc atggatgaaa
     8881 gtttttgtgc gcgacagcaa cgacccactg tcggaaagca aacccggaca aggtggaatc
     8941 aacattattg atctggcaaa tattaacagt tgtagtttta ttgcaactca agatattgga
     9001 agggtccatg ctgacggcac tttcgaaatt ctcggcaggt tcgataatgc agaagtgcgg
     9061 ggatgcaatc ttttaattga ataactattt ttgcagtatg gaaaaagtta ccaacctcga
     9121 ttttattaaa ggattcagca aaggcgacat gagccgcgtt aagaagtatg tcatgatgta
     9181 tatacagagt gctgatgagg aaatgcctgt tattgaaaaa tcatttaatg ataaggatta
     9241 taaaaaactc aaaacatcct cacataccct aaaatcaatt gccggctata tgggaatgga
     9301 tcatgcacag caactgttaa aaaaaattga agatagtgca tctaatgaga gtgtaactga
     9361 agaaatgtct catgatattg cacaacttaa ggagatgata actttatcaa aagatgaact
     9421 aaaagcgttc atagctcaaa attaatttgt attcttgtca tgctaatact actgtcataa
     9481 tatgtcaaaa agctcaacaa tatttatcat tgatgatgac ccaatgatgc aaagtatgct
     9541 taaagatcat ctgtcggcaa tgtcaaactt taccatccac acttttgcca caggtgaaga
     9601 ggcattggct tccggccagt tagttcctga tattgtattt cttgattaca atctcgattc
     9661 taaaattgct aatgcaatgg atggaataga tgttttaaga gagataaaat caaaatatcc
     9721 tgccacagaa gttgtcatgc tttcaggtca ggataaaatt gaagttgctg ttgacaccat
     9781 gaagtacggt gcattcgact atattgtgaa aggtgaaagt gctttctacc gtgcagaaaa
     9841 aacagtgttc aatctattta agttagcaca actgaaaaag aatgccggga tgtacaagat
     9901 tttaaccatc acttttggtg ctgcattgtt actcatgatt attttagtca tcattcttaa
     9961 agctaaagga atgattactg atcatgttgg ctggtactaa tatcacaaaa ccatgaaaac
    10021 tgttcttgca ctcttattga ttttctcact tacatcatgc cgtaaggagg aaaaatatcc
    10081 tatagagcct gttttgttat ataagtcagt gcagttttat gagaaaaaca gtttttatct
    10141 caccacaact tttacagatg gcgatggaga tattgggtta agcgatgcag atactttgcc
    10201 accttatgac cagacaagcc atttctatca taattatttt tgtaattatc agcaactcat
    10261 caacggcagt tggacgccaa taaatttcag cattccctat tattaccgca ttccaatgct
    10321 tacaccaaaa cagcgaaaca agaatatcaa aggagatatt caggttgatg ttaaaaattt
    10381 tctaagtttc acaacgaatg gaattgccga cacattcaga gtagaaattt ctgttgccga
    10441 cagggcactg catgaaagta ataaagtgct ttcagatgct tttgtaatat ataagtaaca
    10501 aaatatttcg agggcgtaaa tgcaggttaa ctcaaaaggt taaattacca cattaacaat
    10561 tcttcccgga acaacaataa ctttctttgg tacattgcca tttgtccatt tctgcatttc
    10621 ttcagaggcc atcacatgtc tttctatttc ttccttatca atacttaaag gcaattgaat
    10681 tttaaatctg gtttttccat ttacagaaac aggataatca aattcgtttt ctattaagta
    10741 cgtttcattc cattccggaa aagttgcaaa agaaacagag ttactgtggc caagcagttg
    10801 ccataattct tctgcaatgt atggcgcata gggtgaaaga attattgcta aaggttcaag
    10861 tacttcgcgc ttattgcatt tatgttctgt cagctcattc acacaaatca taaatgtact
    10921 tacagaagtg ttgaatgaaa atttctcaac atcttcctga acttttttta tagttttatg
    10981 aagcgttttt aattctgcct ttgtagcggt ttcattactt acttcaaatt taccattgtt
    11041 gtgatacagt ttccaaagct ttcttataaa atttgatacg cctgcaatac cattggtgtt
    11101 ccatggtttg ctttgttcaa gaggtcccag aaacatttca tacattcgta agcaatcggc
    11161 accgtatttt tcacaaatcg agtctggtgt tacgacatta taccaacgtt tactcatttt
    11221 ttcaacggca gtttcgcaaa tcagttttcc gttttctgtt acaaattcag cagttgcaaa
    11281 atcatctctc cattttttta aaccttcaat atcaacttct gtgttgtcca ccagatttac
    11341 atcaatgtga atgagtgtag tattgtaatt tgattttaat cctaccgaaa taattttatt
    11401 tgtagcaact tcgcgatgta ccatacaaga tgtaccttgt atcattccct gattgattaa
    11461 tttctgtgca ggctcatcaa tatttatata tcctaaatct tttaatgcct ttgtccagaa
    11521 acgtacatac aataaatgtc ctgttgcatg ttcgctgccg ccaatataga gatcaacatt
    11581 ctgccaatag ttcaaagcat ctttagaggc aaaagaagtg ttgttgtttg catccatata
    11641 acgtaagaag taccaactgc tgccggccca gccaggcata gttgtagttt ctaatggaaa
    11701 cagatttttg tatttccagt ttttagctct tgccaatgga ggttcaccgg tttctgtcgg
    11761 taaaaattta tctacttcag gtaggactaa aggcaactca ttttcaggaa ttgcaacagg
    11821 aatattttct ttataataaa ttggaatagg ctcgccccaa taacgctgcc ggccaaacac
    11881 agcatcacgc aggcggtagt ttgtttttcc tttacccaga ttttgttttt caatttctgt
    11941 tatacaacag ctaatggcag atttcacatc caaatcattt aaaaaaccgg agttaattaa
    12001 ttttccttcc ttggcatccc atgaaccctt accgaagtca tgtgttatat tgggttctat
    12061 cacttgtggt ctatcgagtc ccaaatgtgt agcaaaacga aagtcgcgtt catcatgtgc
    12121 aggcacggcc atcactgcgc cagtgccgta accggcaagt acatagtcgc caatccaaac
    12181 aggaatttta ttgcctgtaa aaggatgaat gacatagcta cctgtaaaca caccactgat
    12241 tcgtgttact tcagcctgac gttcgcgctc acttctgttt tttgttttgg taacataatc
    12301 ctgcacttct gcacgttgtg atgatgttgt tattttatca accaactcat gttccggtgc
    12361 taatgttaca aacgaaacac caaataatgt gtcgggacgt gtagtaaata cttcaatata
    12421 gtcatcagaa ttctgcaaag aaaatctgat ggttgttcct tctgattttc ctatccagtt
    12481 gcgctgtgct tctttcaatg aatcgctcca atcaatattt tctaatccct gcaacaaacg
    12541 ttcagcatag gctgttatgc gcatgctcca ttgcttcatc agtttacgct ctaccggatg
    12601 accaccacgt tcactcaatc cgtcttttac ttcgtcattg gccaatacgg tgcctaatgc
    12661 aggacaccag tttacatacg cttcgcctaa gtatgcaatg cgataggcca tcagtatccg
    12721 gcttttttca gtttcatcaa agtttttcca ttgtgcagca gtgaaatttt ctatctcatc
    12781 atgaacagca ctaatatcat tattgccgtt tttttcaaat tctgaaatca gtttgtcaat
    12841 tgattcggca cgattacttt tattgttata ccatgcatca aacaatttta aaaaaatcca
    12901 ttgagtccat ttgtagtagg aggggtcaga ggtgcgcact tctctgtccc aatcgaatga
    12961 aaaacccatc ttatccaact gttcacgata gcgattgatg ttggtctcag tggtaattgc
    13021 cgggtgctga cctgtctgta ttgcatactg ttctgcaggc aatccaaaag aatcatatcc
    13081 cattggatgc aaaacattaa aacctttcag tcttttatag cgtgaataaa tatcagaggc
    13141 aatataacct aacggatggc caacatgtag cccggcaccg gaagggtagg ggaacatatc
    13201 caacacataa aactttggct tgtcagattt attttcaacc ttaaaagttt tgtttgcagc
    13261 ccaatattgc tgccattttt tttctgtttc cctgaattga tagtccatta tttgcgctcc
    13321 gaaatgatgc gctaaaatac tgaaatactg ctgatgtttg tattctttat tgacttaaaa
    13381 agtcacgcac cgttgtaagc atacattccg ggttgtcggc atgaacccaa tggccggcat
    13441 tggcacaggt ttttatttct gcttcaggga ataagtttaa tatttcagac ttatcatcat
    13501 cggttatata cttcgatttc tcacctttaa taaataaggt tttcccctcg aatttttctg
    13561 ttgatgtaat tctttcgcct accgattcaa ttttttctgc aattactttc agattaaaac
    13621 gccattgtaa acgatcgcct tcccagtata aatttttaag caaaaacatt tttactccgg
    13681 catcatcaat atatccagac agaatatgtt cggcttcttt tcgggtgtta accgtatcga
    13741 gatctactgc ctgaagtgct tttattatct gctgatgatg tggtgcataa taacgtggtg
    13801 caatatcaac aactatcaaa gattttaaaa tatcctgatg cttcaaagca aatttcattg
    13861 ctactttgcc accaagagaa tgccccatga tgtttacatt ttgtaaatcg tgtttactga
    13921 taaaagcgaa aacatcatca gccattgaat cgtaatcaaa cacagagtcg tgtggcgaac
    13981 ttccatgatt gcgcaagtca acggcataca ctgctatatt attttccgtt atttgttttg
    14041 aaaaagattg ccagttatca ttcagcccga ataaaccatg caaaataatt aacggctgac
    14101 cattaccact gctgcgataa aataaatcaa gcgacattat tttctgatta ctgctccgag
    14161 atttttctca aaagcttttt caatcttctg cattactgat tctatttgtt tatccgttaa
    14221 tgtagcctca tcatcagcca aagtaaaact caatgcatac gatttttttc ctgcttcaat
    14281 tttttcgcct tcatacacat caaagatgtc aacctgtttc aataatttct tttcttgctt
    14341 taatgctaaa tctctcaggt cggcataatt tattgcctga tcgagcatta gtgaaaggtc
    14401 gcgttttacc tccggaaatg gttgtggtaa tttgatagca gttttatgcg ataaactttt
    14461 ttccagcaaa acatctatat tcaatactgc aagataaacg ggctgtgcaa tatcgaattg
    14521 tttcaaaata tttttcttta ctgatccaat atacccaaat gctgactgcc tttgcatgat
    14581 gtttgcgaaa gttgaaaaca aagtatgact atcctgcacc caatttaaat gagagcttat
    14641 gcgaggcatc actaactcaa tagctttttc tgcaacagat ttaagataaa ataatgacga
    14701 ttctttttct gacttatgcc agttaggttc attcaccata ccggtcatag caattaccaa
    14761 attgtttgtt tctttaagtt ttccttcatg ctgaagatag gtcttaccaa attcaaacaa
    14821 ttttaaatta tgattttttc tattgatatt ataacaaata gtgtgtaggg tagaagcaac
    14881 catatcgggg cgcaatatgt ctaactcctt acttaacggg tttaaaactt tcacagcctc
    14941 atcaccggca gatgaatgtg aggatgatac taaagaatta ttaatcattt caaaaaaccc
    15001 gatagagctc agatgatgtg aaattttata ttgcattgca ggttttctgt caggggtgtg
    15061 acttgaaaag gatatacccg ttttttcatt caatggaatg tgatggtaac cataagtccg
    15121 taaaatttct tcaacaacat cggcctgtcg ggtaacatcg gttttatatg tcggtatgga
    15181 aatggttgca ccatttacat tgctgctttt aatactgaag ccacaatact ccaacacaga
    15241 aagcaacata tcattactga aattaattcc tgatagctta tttatttttt caaagtttac
    15301 atatataatg cgtggttcaa caacttcagg atacaggtca acaacatcgg atgttatttt
    15361 tccccctgca acttctttta taagtaaggc tgcacgtttc aatgcatata gtgttccatt
    15421 aggatctgtg cctctttcaa aacgaaatga cgattctgtc tttaatccat gatgtttggc
    15481 agctttacgc acttcggcag cattaaacca ggcactctct aaaaatattt gtgttgtaga
    15541 agcagacaca ccggagtcgg tgccacccat cacccctgca atacacaatg gttctgtggc
    15601 attacatatc atcacttcat cacctgtcag ttttctttca atgtggtcga gtgtagtaaa
    15661 tacagatgcc ttacctgttt tctgcacaat aatttttcct ttcactctgg ctgcatcaaa
    15721 ggcatgcaat ggctgcccgg tttcaaacat cacaaagttg gttacatcaa caatgttgtt
    15781 aatgcattta actcctatgg actgcagtct gcttttcagc cattccggtg attcgcctac
    15841 tgttacacca ctaatagaaa ctccgcaata tcttccgcag cctgaatttt tatctacaac
    15901 tacgtcaatg cgcagtgcgt tgttttcaga tttaaaagca cccacatcag gcatactgaa
    15961 ttgtgctgtt gttgaattat tgttttctgc ttttgctctt gtattgatta cagcagcaag
    16021 atcgcgtgcc acaccaccat gcgaggctgc atcaatgcgg tttggtgtca gacctatttc
    16081 aaaaatatga tcctgtttaa ttttaaacca ttcagcagca ggagtgcccg gcacagcatt
    16141 ggcatctaaa accataattc cctcatgcga atttccgagt cctatctcat cctcagaaca
    16201 aatcatcccc tctgaggcct gcccgcgtat tttagaattc tttatgtcga atggttcgcc
    16261 aaaggttgga tacaatcgtg ctccattcaa agcaaccaat actttttgtc ctttggcaac
    16321 attggcggca ccgcaaacaa tctgcaaggg aatagttttg cctacattca ccttcgtaac
    16381 actcagtctg tcggcatctg gatgtttttc cttctccaac acttcgccaa caaccaagcc
    16441 acgcagtccg cctttaatgc tgtcggtttc gtccagcgac tccacttcaa gtccgctgct
    16501 tgtcagtaaa tgttcaattt cttcaggtgt ctgttgtaac tttatatagt ccttcaacca
    16561 gtcgagcgat attttcattc ttatttttaa tgaaagtgaa aatgcaaaat taaattattc
    16621 gggcagacaa aaatattcag ctattatccc tgctaaaggt cgcttccaaa accgctgtta
    16681 tgctgatggt cttttttgtc ttgttcatac ttgctgcagt ctgtctctat actcagcgga
    16741 tgttccggtt tttcaaaatc accgttgtac aaattcaggt ctttatcggc ataagccttt
    16801 ttcatataca atgcccatat aggcaatgct gtatttgcac cctgaccaag gctggtagtt
    16861 ctgaaatgca ctgagcggtc ttcgcaacct acccacacac ctgtcaccaa tgttggtgta
    16921 atgcccataa accatccatc actctgattt tgcgttgtac ctgttttacc tgcaatggga
    16981 tttgtcaatc caaaacgaaa acgcaaacgt ccacccgttc cactttgtac aacgccctgc
    17041 atcagattca gcatcagata agctgtctct tcgcttatgg cttcaacctt gcgtggcaca
    17101 aactcttgca gcaccatgcc gtttttatct tcaattcgtg taatgtaaat tggctctgtc
    17161 cacacacctt tattggcaaa tgtgctgtat gcaccacaca tctcaaacaa actcacatca
    17221 ggtgtgccta aacaaattga aggcacttcg tcaatgggcg atgtaatacc cattttcctt
    17281 gcaatctcta tcatcggttt tggtccaaac tgtttcatca gataggccga cacactattc
    17341 accgactcgg caagagcttc cttcaatgtc agcatgccgc catattttcc atcactgttc
    17401 gatgggctcc agtcgggctg tccgggtata ggaatcgtca cacgcacatt agggacttta
    17461 aagcaaggcg aatagccttc ctgcatggca agcgtatata aaaacggttt aaatgttgaa
    17521 cccacctgcc tgcgtcctgc cttcacgtgg tcgtatttaa agtagcgata attgtttccg
    17581 ccaacccatg cacgcacata gcctgtctgt ggctcaatac tcatcacacc ggcttgcaaa
    17641 aacatcttgt aatacttcag cgagtcaagc ggactcatca ttgtatcaat atcgcccttc
    17701 cacgaaaaaa tcgtcatcgg tattttagtg gcaaaatttt cttttatctc ctgctcactc
    17761 aacccttcag ctttaagcga aatatagcgg tcgcttttct taataccgtc ctctataact
    17821 tccggatgtt gcgcccacgg cactcgaccc ttccaatgct gataaaaagt tttttgcaac
    17881 tctgtcagat gttccttcat cccttcttcg gcatactcct gcaatcgcga atctatcgta
    17941 gtgtaaatgc gcaaaccgtc acgatacaaa ttatagctgc tgccgtcaga ttttttatgc
    18001 tccttgcacc acttcaccag ttccaatcgc aaagtctctc ggaaataagg tgccagtccg
    18061 tcattatgat cctctgccct gaaatttaat tttatcggca gcttcactat agagtcgtat
    18121 gctgcctcac tcaaaaattt atacttctcc atctgactca acaccgtgtt gcgtctaatc
    18181 aatgcattct tcgggttacg caccgggctg taacgtgtag gtgcctgcag caaacccacc
    18241 agcatggccg actcttccac cttcaactga gatggcatct tattaaaaaa agtctttgct
    18301 gccgatttta ttccatagct gttatgacca aattcaaccg tattcagata catggccata
    18361 atctcctgct tggtatagtt gcgctccagt cgcacagcaa taatccactc cttaaccttg
    18421 cgcactgcta accgaaaacc gctctcattc tcacgtggaa acaagttctt ggccaactgt
    18481 tgtgtaattg tgctgccacc acccgaagaa cgctgctgca aaatcaccgt cttaaaaaac
    18541 accctcccca atccgcgcat atccacaccg ctgtgctcct caaaacgaat atcttccgta
    18601 gcctttagtg catcaaccgt attttcggca agctccttat aatgaatgtt cgaacggttc
    18661 tgaatataat atttgcccaa caaaacatta tcgctcgaga ttacctccga tgccagactg
    18721 ctgttcggat tctccaactc ctcaaacgta ggcaactcac caaacttacc catgttcact
    18781 gcacccaaca ccagcacaat tattgccagc ggcaacagtg ccaccaccca catcagcaaa
    18841 acaaacatgc ggaaaacccc gcgctttttc ttattggtca tattccccat tctgtcagaa
    18901 ccggcaaaac taaactttcc actcgtaaca taacgccaga gctaagtttt tattcaaaca
    18961 acgccattaa cagcaaaagt gatattggaa tagaggatat ttgtattaaa caagaacacc
    19021 gaattatttt ttgataattc ggtgttcttg ttttaaaact ttacagtgtc gaagaatgaa
    19081 ttagaaaaaa taattcatcg ttaataaaaa tttattcaac aaccaatttt atggtttgtg
    19141 actgttgtgg agatataact ctgatgaaat ataatcctgc cgatggtgga agagaaaata
    19201 gttgagtcga attaataatc cttctactaa ctagacttcc ttctgaagta tacaattcta
    19261 ctgtaatgtc tgaacctaaa tcttcaggta attgtattgt aa
//
LOCUS       DAORQT010000009        18282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_245_length_18282_cov_14.969276, whole genome shotgun sequence.
ACCESSION   DAORQT010000009 DAORQT010000000
VERSION     DAORQT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18282
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_18282_cov_14.969276"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PK134_00770"
     CDS             <1..1237
                     /locus_tag="PK134_00770"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRB37418.1"
                     /translation="ESKQYKDSMDRINNKFSFFNFLLAGYSYRHSYREMTYTIKPIAN
                     TFSFNTVEGWNVALNTEFVKRLPNRKRLTVFTNTRYGFSNTHWNANAGFKYLFNRFSD
                     NTISVEGGTAVNELNSTSPVKPLVNMLYTLWAERNYLKIYEKQFFKIAYGREWFNGFN
                     ATAEIEYGRRNTLKNTTDYTWKNFKNREYTSNNPINPDKDIKNFRPHHYFNTHLSLTY
                     SPFTKFITRPDGKVREESGWPVFFVNINKAFGNVINSKTDYLSFDAGFNHQIDFGMLG
                     YVVYNCRYYRFVNRKDVEFPDFFHFAGNKTIVSDFKAEDFAYLDYYKYSNMKSAFAIH
                     AEYNLNRFITNKIPLIRKLKLNEVVGFHFLKVSEMKSYYELTVGLEKLGVIRADFVCS
                     YDGKASPFYSFRFGLKLRQ"
     gene            1297..>1613
                     /locus_tag="PK134_00775"
     CDS             1297..>1613
                     /locus_tag="PK134_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37419.1"
                     /translation="MKKQVLIFIFCLIGTFVRGDAKHLYSVFDRNDLPVFSRPFFITI
                     TADPIALKSAERMLGDAIKSNRQQEIFQNANNAGYYNLLNGDFLQALKFLQIAVNHKP
                     LSK"
     assembly_gap    1614..1636
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1637..3460
                     /locus_tag="PK134_00780"
     CDS             <1637..3460
                     /locus_tag="PK134_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB37420.1"
                     /translation="AFALDVAGRHEQAFSIYKELLEKDLANSTQIRAYVQAMAGLIFL
                     QHADTVSAKPLLGNALQYFSANNFLLQQYYLYERMGDINLVAKNFNNSSANYLQQLKY
                     ATKLNNKTFQALATRNLGLCNLKRDDYLKAVSYFRQSLDFRDNVLLQKLLKDTYLKIV
                     TISSFNNDFEKADRYHELYRKLKANETVVKETAALQAEKEKILFLLSQSANNENNMVS
                     RQEYELSQQLTTLDIERQNKEKALEELTLAEAQKKLKELELDKIAGEKLKQEAELAKK
                     ELQISRQKEFRNILLGISGLIVLGIVFLINRYRLKRKSLEQLRQAHQELRQAHEQLKQ
                     TQEQLVQSEKMASLGQLTAGIAHEIQNPLNFVNNFSTLSMDMIDDYLKSKDETLLDEL
                     KSNLKRINHHGGRVSSIVKGMLLHSRNKTAEKELSDINLLVDESLALAFHGKKSTEAD
                     FYCHIEKDFDATAPPVKVLPQELRRVIINLINNAFYAVHERAVKSNANKEEVKYKPQV
                     SVTTHVAGQFLEITITDNGTGIPDTIKDKIYNPFFTSKPTGKGTGLGLSVSFDIIEKQ
                     HGGKLKFESEQGVGTVFTISLPLNAPPDLKQSNGDVKKSVV"
     gene            3477..4634
                     /locus_tag="PK134_00785"
     CDS             3477..4634
                     /locus_tag="PK134_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37421.1"
                     /translation="MPLTVFSQSDTLNRATNIVGCQSGDCVNGIGVYLYKNGNRYEGA
                     FKNGSPDGKGIMNYRNGNRYEGDFTQGRLNGKGNYNYRNGNSYTGDFVNDQFHGRGIY
                     IYNSGNQYIGDFFEGSKHGSGTFTFNDGSTYTGQFVDDMVQGKGTWRYANGDWYEGDF
                     VNSKKSGKGTYYYNDSSRYSGDFVNDVYNGLGVFTFADGSTYIGQFRNDLFNGQGTCY
                     YSNGDVYTGEFRDGLYSGGGVYAYATGDRYKGTFLNNVFNGEGTYIDESGEETVTMYE
                     NGRAVGESKVIDKENVKPVPEENKTEAKKPEDKKEEPKVSEPTVAKIPCPACNGKGKI
                     LQAEVKRNRSVVKDISNGLGPRNFVTYTVSEVVKPAAWATCKVCHGTGIKQ"
     gene            4652..7996
                     /locus_tag="PK134_00790"
     CDS             4652..7996
                     /locus_tag="PK134_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRB37422.1"
                     /translation="MPSKAIQKVTRLFWLFLLIAINAFAQKSDVFRHLNTVNGLSHNR
                     ITCIIQDKIGFIWIGTEDGLNRYNGYKIDVYKHNPSDETSVSSNGIRCLFEDSKGKLW
                     IGTDDGLSFFDIETEHFGSFRTNTKGNSISSNQITCMEEDASGVLWIGTAGGGINLFN
                     PSTGKWQNFKSDKSNPNSICSNNITAIVKDKNGVLYLGTDNGLAIADRNQPGIFTIID
                     HDPANPNSPLENSITCLYADFQNKIWIGTLNSGMSAMRTDNRSFTHYTNLGKYANNSV
                     FGINKDLDGSIIAGTIGGGLIIFNSDASTYSSFTHSKTDPFSISSNSIWTIYRDKAGI
                     IWIGTDNGLDYYKEDLMRFKTELPLPDDPLSNATPNKNVFCFLPEGNNLWMGVLGTGV
                     VVKESTGTIKQVYNAANGLADNSVFCLIKDRNGIVWVGTYNGLTSINPQSGVVRSYRI
                     ADGLTNSNIRSLAEDENGNIYIGTYGGGLCVLNRQTDKISSIRKEDGKGLTNNIITAL
                     KYDGNGNLFIGTYGGGLCLLNISANTITAFESKPGSNTTISSNYVNSIYSFSNQQLLV
                     GTYSGGINIFNYATKSFTALTETNGVPNNNVTNIVSDAEKNIWFTTGNQICKMKLAPS
                     DSILTMRIYDEQDGVFNRFNPGAFAQTADGKIYFGGISGINRFLPSAIRDNPYIPPVV
                     ITKFYLFEKPYFMDTIIMSKNMIELTYNQNFFSFEYAALNYILPEKNQYAYMLEGLEK
                     TWTYCGSKRDRQYTDLDPGTYKFRVKACNNDGIWNQDGTYIIIIIKPPFWKTWWFYIL
                     SGLTFVGLTITYIRWRTRTLMKQYEILEQKVQERTAELREEKEKSEQKSLEIERALKE
                     LKDTQSQLIHAEKMASLGQLTAGIAHEIQNPLNFVNNFSELSQDLIDELNATDDKEEW
                     KSISHDIKTNLEKINEHGKRAERIVKSMLMHSRSQTAEKVLTDINKLAEDVMNLAYTS
                     LRANDSNFGCKNMIELDASLPKLSIVQQDISRVLLNIFNNAFYAVNQLRKKSDKAFHP
                     EVRLRTWKAANKVYISISDNGGGIPDDIVDKIFQPFFTTKPTGEGTGLGLSLSYDIIT
                     KGHYGMLSVNVQKGIGSEFVIELPQL"
     gene            8007..8390
                     /locus_tag="PK134_00795"
     CDS             8007..8390
                     /locus_tag="PK134_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB37423.1"
                     /translation="MQTKILIVDDERDVELLFRLKFRREIKEKHIDLHFAFSGEDALN
                     YLHNINPMDVLLVLSDINMPGMNGLQLVEKVKAEFPAMRIIMVSAYGDDKNYNEAKAK
                     GVDDFVTKPVDFEDLKRKMARVITI"
     gene            8393..9994
                     /locus_tag="PK134_00800"
     CDS             8393..9994
                     /locus_tag="PK134_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB37424.1"
                     /translation="MAKKILVVDDEPDLELLVKQRFRSQIRNNELQFDFAHNGEEALQ
                     KLKDDVDIDLIFTDINMPVMDGLTLLNQIKENKIQPKAVVISAYGDMDNIRKAMNSGA
                     FDFITKPIDMTDLDTTLRKGLSEIEIIKKGQKAQEALQQTIIEKEIAVLEKDKAEESK
                     RLKQQFLANMSHEIRTPMNAIIGTVNLLHKSNLDDKQSKYIGIIKTSADNLLVIINDI
                     LDISKIESGKFTIEEIPFNLHEKVNHVYESLKVKAEEKGLAFNVDYCSTLSDNYIGDP
                     VRITQVLINLTGNAIKFTEKGSVTIRVASIADVPNGVSISVVDTGIGIAQDKIQHIFE
                     SFTQESSDTTRKFGGTGLGLAISKQLIELMHGELVVESTQGKGSSFIMKLPLVATNEL
                     ATETKDKVSSSVEIRKGLRILLAEDNQFNQMVAEDTLRMVIPDLTLDIVDNGVQAIEK
                     LTSGNYDLVILDIHMPEMDGYETTQFIRTQMPSPLNGVPIMAMTANVISEEIDKCFAV
                     GMNAYVSKPFTHEDLFDKIKSLTSK"
     gene            complement(10046..11803)
                     /gene="sppA"
                     /locus_tag="PK134_00805"
     CDS             complement(10046..11803)
                     /gene="sppA"
                     /locus_tag="PK134_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRB37425.1"
                     /translation="MKQFLKYVLATLTGLILFCLLTFFLFAVMLGSAFKKEVVEVKSN
                     SVLKIEFKMPIAERSDNNPFRNLNLSTLKASKKPGLNDIVANIEKAEKDDNIKGILLN
                     LNYFEAGYATTTEIRNALVKFKKSGKFIFAYAEYYSTKAYYLASVADKIYVHPEGAVD
                     FKGLFTQMMFMKGALDKLEIEPEIIRHGKFKSAIEPFILDKMSPENKEQVATFINSIW
                     ETISSEIAESRKISREELEQIADSLSAENAKDALRLKLVDQLCYKDELEKILAEKSGV
                     NDDDINYISLNKYNEAVVKSDKKFTTDRIAVVYAVGDIVSGSGEADQVGSDRIAEAIK
                     KARLDDKVKAIVLRVNSPGGSALASDVIWRETKLAKEKKPFIVSMGNLAASGGYYISC
                     LADTIVAQPTTITGSIGVFGLLFNAQKLLNNKLGLTFDTYKTGPYADLGMPTRPLEPA
                     ERNILQRSVEDVYSTFIGRVAEGRKMETVMVDSIGQGRVWSATDAKRIGLVDIYGGLN
                     DAVTLAAKKAGLDNYRIKELPEQKDPIQEILEDMKGESESMMMPDYMKESMKLMKYLK
                     QISEQDKIQARMPFGIAVE"
     gene            11861..12370
                     /gene="folK"
                     /locus_tag="PK134_00810"
     CDS             11861..12370
                     /gene="folK"
                     /locus_tag="PK134_00810"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRB37426.1"
                     /translation="MALHQNTAIILAGSNLGNRLHNLTEAALFLQRLSTKKMASSSVY
                     ESAPWGNQNQPPFLNQAFSIYNAFTAHELLDVLLTFEKSQGRTRKHQWEPRSIDLDIL
                     FFNNEIICTPELTVPHKHLHERRFVLEPLAELISDFIHPVFKKSISELLVECKDDLKV
                     SKYPLYVQK"
     gene            12357..12974
                     /locus_tag="PK134_00815"
     CDS             12357..12974
                     /locus_tag="PK134_00815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRB37427.1"
                     /translation="MYRNRFLTIEGNIGAGKTSLAKMIAEEFNYHLILEEFAENTFLQ
                     QFYDSPEKFAFPLEMSFMAARYKQLKTFFNKNTLPFPIVSDYMFQKTLLFAKSNLKKN
                     EYGLFEQFFQMLNVGLPLPQLIVYLQKEIPSLQKNIKKRGREYEQNMKDDYLLKINNA
                     YSRFMKKSNTASNILIINTEGLDFVRKRSDFQKIVETIEKTGFRF"
     gene            13091..14545
                     /locus_tag="PK134_00820"
     CDS             13091..14545
                     /locus_tag="PK134_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB37428.1"
                     /translation="MKVKTLLVSAFLISATQFAFAQESARYGLPKNKDFDKWSAGLTV
                     GLNWFQGDLNEKTGNNNSILNNIQDPVFGLKVGYQISHSVQVNVRGNYTNLSGKKNKT
                     TFRVPNAVTSPQITRNIKFKSPIYQASLNLNYTIGNISFLQRNKRLHFYGEIGLGIFS
                     YAPKVTDLDNGTVYVKKGSVAEGFLPLSLGFKYQIKRFDVGLLATFNKTLNDKVDQVY
                     DSKTESDNWSFFLLGVNYTFGKKQAMMEWVNPMEVVYNDLSDMKDRIDVMSGDKDKDG
                     VSDLFDKDNSTPEGVKVYGDGTALDSDGDGVPDNVDSDPYSNKGAKVDASGLEVDTDG
                     DGVPDSRDMEPNTPKGNLVNFQGMTIAPAGSYGNDGITSNSATGWLPSIFFDVDQSAI
                     KSVYNDRLNTVARVMIKNPKLKLSVVGNCDVDASEQYNDKLGMKRAEAAINHLVKVYG
                     IDASRLTATSLGKKEQLAKGNKRMNRRVDFEVAK"
     gene            complement(14592..15122)
                     /locus_tag="PK134_00825"
     CDS             complement(14592..15122)
                     /locus_tag="PK134_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRB37429.1"
                     /translation="MKKLTGSEMGRTSTEGFKQIQKIPVVIILENIRSALNTGSVFRT
                     CDAFPVEKIILTGITAQPPHKDILKTALGATESVNWQYYSDIKVCISKLKANGYKIFA
                     AEQTSQSIAMPDFKPEKDEKLAVVFGNEVDGVSNETIAMCDGAIELPQHGTKHSLNIA
                     VCAGIMIYDLFQKMKA"
     gene            15171..17756
                     /gene="mutS"
                     /locus_tag="PK134_00830"
     CDS             15171..17756
                     /gene="mutS"
                     /locus_tag="PK134_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRB37430.1"
                     /translation="MQQYNAIKAKYPDALLLFRVGDFYETFSEDAVKTSKILGIVLTK
                     RSNGAASEMELAGFPYHALDTYLPKLVRAGQRVAICDQLEDPKTTKTIVKRGVTELVS
                     PGVTYNEKILENTHNNFLAVVHLTEDVSGVAFADVSTGTFFTARGKDAYIEKLLQNFK
                     PSEVVLQKSMQKDFIARFGDGFYIYTLDQWAFQKEYAEEVLLKHFQTNSLKGFGIHEM
                     DEMIVACGVALHYFGQMQQQTLSHITGIARIDEEQYVWLDKFTIRNLELVASPHENAR
                     TLVDVIDRTVTPMGSRLLKRWILLPLKDIQTIHERHDAVAAFTLDETLRGQIATSLRH
                     VGDIERMLSKIALRRISPREMVQLKRALSQIQPVKDNLATAGNSTFNVLSEQLNPCNL
                     LIQKIQDALIEDAPPLASKGGFIAKGFSQQLDDLRQVAFSGKDYLLKIQQREVEQTGI
                     SSLKIAFNNVFGYYIEVTNAHKSKVPESWIRKQTLVNAERYITPELKEYEDKILGAEE
                     KISTIENEIFQELISFTQNYIQPLQVNASVLSQLDCLLSFANVAIENNYTRPVVNDSL
                     VLSITEGRHPVLEKQMPPGEAYIPNSIFLDQQTQQIIMITGPNMSGKSALLRQTALIV
                     LMAQTGSFVPAKSAEIGWVDKLFTRVGASDNISSGESTFMVEMNETASILNNITQRSL
                     VLLDEIGRGTSTYDGISIAWSIADYIHESKNARAKTLFATHYHELNEMTNSYNRIKNY
                     NVAIKEAGNKILFLRKLVPGGSEHSFGIHVARMAGMPPHVINKAEKMLALLEKAHSNT
                     ELLRNAGHQADKDAMQLSFFQLDDPLLEQIRDEILKTDVNTLTPVEALFKLNEIKTLL
                     SKNKK"
     gene            17844..17916
                     /locus_tag="PK134_00835"
     tRNA            17844..17916
                     /locus_tag="PK134_00835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17877..17879,aa:Gly,seq:gcc)
     gene            18015..18101
                     /locus_tag="PK134_00840"
     tRNA            18015..18101
                     /locus_tag="PK134_00840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18049..18051,aa:Leu,seq:taa)
BASE COUNT         6000 a         3222 c         3524 g         5513 t
ORIGIN      
        1 agagagtaaa cagtataaag atagtatgga tagaataaat aataagtttt cgttctttaa
       61 ttttttgctt gccggatatt cctacagaca cagttaccgg gaaatgactt atactataaa
      121 gccaattgcc aatacatttt catttaatac agtggaaggc tggaatgttg ccttgaacac
      181 agagtttgtt aaacgtttgc caaaccgaaa acgcctgact gtttttacca atacacgtta
      241 tggttttagc aatacacatt ggaatgcaaa tgctggtttt aaatatttgt ttaaccgctt
      301 tagtgataat acaatttctg ttgaaggcgg tactgctgtt aatgaattaa acagcacaag
      361 ccctgttaaa cctttagtca acatgcttta tacgctatgg gccgaacgta attatttaaa
      421 aatttacgaa aagcaatttt ttaaaatagc atatggtaga gaatggttta atgggtttaa
      481 tgcaactgca gaaattgaat atggccgaag aaatacattg aaaaacacca cggattatac
      541 ttggaagaat tttaaaaaca gagaatatac ttccaataat cccattaatc ctgataagga
      601 tataaaaaat tttcgacctc atcattactt taacacacat ctatcattaa cctactcacc
      661 atttacgaag ttcattacaa ggcctgatgg aaaagtgaga gaagaatcag ggtggccggt
      721 attttttgtg aacatcaata aagcttttgg caatgtaata aacagtaaga ctgattatct
      781 ttcatttgat gcaggcttca atcatcagat agattttgga atgttaggtt atgtagttta
      841 caattgcagg tattatcgtt ttgttaatcg aaaagatgtt gaatttcccg acttttttca
      901 ttttgcgggt aacaaaacta tcgtctcaga ttttaaggct gaagattttg cttatttaga
      961 ttattacaaa tattcgaata tgaaaagtgc ttttgcaatt catgcagaat acaatctgaa
     1021 tcgttttatt accaataaaa ttcctttaat cagaaagttg aaactcaatg aagttgtagg
     1081 cttccacttc ctgaaagtta gtgaaatgaa aagctattat gaacttactg taggattaga
     1141 aaagctcgga gtaatacgag ctgattttgt ttgcagctat gacggcaagg caagcccgtt
     1201 ttattcattc cggtttggac ttaagcttag acaataagat tgaaagttac atcgtcaatt
     1261 gataaaaatt gaatacattt ggcattcgtc tcggtgatga agaagcaagt tttaattttt
     1321 atcttttgtc tcattggcac atttgttagg ggtgatgcaa agcatcttta ttcagttttt
     1381 gacagaaatg atttacctgt tttttcaaga ccttttttta taactataac tgctgaccct
     1441 attgcattaa agtctgcgga aagaatgtta ggcgatgcaa tcaagagcaa ccgtcagcag
     1501 gaaattttcc aaaatgccaa taatgccgga tactacaatc tactcaatgg tgattttctt
     1561 caggcattaa aatttttaca aatagcagta aatcataagc cactgagtaa ggannnnnnn
     1621 nnnnnnnnnn nnnnnngcat ttgcacttga tgttgcaggt aggcatgaac aggcattttc
     1681 tatttataaa gaattactcg aaaaagattt agccaactca acacaaatta gggcctatgt
     1741 tcaggcaatg gcaggtttaa tttttcttca acatgcagat acagtttcag caaagccatt
     1801 actcggcaat gcattacaat atttttcagc caataatttt ttacttcaac aatattatct
     1861 gtacgaaaga atgggtgata ttaatcttgt tgcaaaaaat ttcaataatt catctgctaa
     1921 ttatttacaa caattgaagt atgccacgaa gttgaacaat aaaacatttc aggcattggc
     1981 tacccgaaat ttaggtttat gtaatttaaa gcgtgacgat tatttaaaag ctgtatccta
     2041 tttcagacaa agtttggatt ttagagataa tgtccttttg cagaagttgc taaaagacac
     2101 ctatcttaaa attgtaacca tcagcagttt taataacgat tttgaaaagg cagatcgcta
     2161 tcatgagtta tataggaaat taaaagctaa tgaaactgtt gttaaggaaa cagcagcatt
     2221 gcaggcagaa aaagaaaaaa ttctgttttt attaagtcag tcggccaaca acgaaaacaa
     2281 tatggtttcg cggcaggaat atgaacttag tcaacagtta actacgcttg acatcgaacg
     2341 tcagaataag gagaaggctt tagaagaatt aacacttgcc gaagcacaaa agaaactgaa
     2401 agaacttgag ctggataaaa ttgcaggaga aaaattaaag caagaagccg aacttgctaa
     2461 aaaagaactg caaatcagca ggcaaaagga atttagaaat attcttttag gtatttccgg
     2521 attgatagtc ttgggaatag tatttttgat taaccgctat aggcttaaaa gaaagtcatt
     2581 agagcaactc aggcaggctc atcaggaatt gcggcaggca catgagcagc taaaacaaac
     2641 acaggaacaa ttggtgcagt ctgagaagat ggcttcttta ggtcagctta ctgcaggtat
     2701 tgcacatgaa attcaaaacc cgttgaattt tgtaaataac ttttctactt taagtatgga
     2761 tatgattgat gattatctga aaagtaaaga tgaaacttta cttgatgagt tgaagtcgaa
     2821 tctcaaacga attaatcatc atggaggaag ggtttcttct attgtaaaag gtatgttgtt
     2881 gcatagtaga aacaaaacag cagaaaaaga attgtcagat attaaccttc ttgttgacga
     2941 atcattagca ttagcctttc atggcaagaa atcaacagag gcagattttt attgtcatat
     3001 tgaaaaagat tttgatgcaa cagcacctcc agttaaagtt ttaccccagg agttgcgtag
     3061 agtaattata aatcttatca ataatgcctt ttatgctgta catgagcgtg ctgtaaaatc
     3121 aaatgctaat aaagaagaag ttaagtataa accacaggtt agtgttacaa cacatgttgc
     3181 cggccagttt ctggaaatta caattactga taatggaact gggataccgg ataccatcaa
     3241 agataaaatt tataatcctt tctttacatc caaaccaaca gggaagggta cgggattggg
     3301 gttatcagta agtttcgaca taatagaaaa acaacatggg ggtaagttaa aatttgagtc
     3361 agaacaaggt gttggaacgg tttttacaat ttctttgccg ctaaatgcac cacctgatct
     3421 aaaacaaagt aatggagatg ttaagaaatc tgttgtataa tttatttttc gtgttaatgc
     3481 cattgactgt tttttcgcag tcagacacat taaaccgtgc aacaaatatt gtaggttgtc
     3541 aaagtggcga ctgcgtaaat ggcataggcg tttacttata taagaatggt aatcgttatg
     3601 aaggtgcttt taaaaatggt agtccagatg gtaaaggcat tatgaattac agaaacggaa
     3661 atcgctatga aggagatttt acacaaggca ggttaaatgg taaaggtaac tacaattaca
     3721 gaaatggaaa tagttataca ggtgattttg taaacgatca gtttcatggt cgcggtattt
     3781 atatttacaa cagcggcaat caatatattg gtgatttttt tgaaggaagc aaacatggca
     3841 gtggaacatt tactttcaac gatggaagta catacaccgg tcagtttgta gatgacatgg
     3901 tacagggtaa aggcacctgg agatatgcaa atggcgattg gtatgaaggt gattttgtaa
     3961 attcaaaaaa atcaggtaag ggtacttatt attataatga cagtagtcgt tatagtggcg
     4021 attttgtgaa tgacgtttac aatggcttgg gtgttttcac gtttgctgat ggaagtacat
     4081 atataggtca gttcagaaat gatttgttta atggtcaggg aacatgttat tattctaatg
     4141 gtgatgtcta tacaggagaa ttcagagatg gattatacag tggaggtggt gtttatgctt
     4201 atgccacagg agatcgttat aaaggaactt ttctaaataa cgtttttaat ggtgaaggaa
     4261 cttatattga tgagtcgggt gaagagactg ttacaatgta tgaaaatggt agggcagtag
     4321 gagaatcgaa agtcattgac aaggaaaatg tgaaaccagt gccggaagaa aataaaaccg
     4381 aggcaaagaa acctgaggat aaaaaagaag agcctaaagt ttctgagcca actgttgcta
     4441 aaattccatg ccctgcctgt aatggaaagg gaaaaattct tcaagcggag gttaagcgaa
     4501 accgctcagt tgttaaagat atcagtaatg gtttaggtcc ccgaaatttt gttacctaca
     4561 cagtgtctga agtagtgaaa cctgcagcat gggcaacatg taaagtttgt catggcacgg
     4621 gaattaaaca ataaatctta cttttgaacc catgccttca aaagcaattc aaaaagtcac
     4681 ccgccttttt tggttatttc ttctaatagc catcaatgct tttgcacaaa agagtgatgt
     4741 gttcaggcat ctcaacactg taaacggact ttcgcacaat agaattactt gtatcattca
     4801 ggataaaatt ggatttatct ggattggtac tgaagacgga ctgaacagat acaatggcta
     4861 taaaattgat gtttataagc acaacccatc tgatgaaact tctgtttcga gcaatggcat
     4921 tcgttgtttg tttgaagaca gtaaaggaaa gttgtggata ggtacagatg atggactaag
     4981 ctttttcgat attgaaactg aacatttcgg aagtttcaga acaaacacca aaggaaattc
     5041 aatcagcagc aatcagataa cctgtatgga agaagatgcc tctggtgttc tttggattgg
     5101 aactgccggt ggcggcatta acctttttaa tccttcaacc ggaaaatggc agaattttaa
     5161 atccgataag tcaaatccaa attcaatatg cagtaataat attacagcta ttgtaaaaga
     5221 taagaatggt gtattgtatt taggtacaga taatggtctt gcaattgcag atagaaatca
     5281 acccggtatc tttactatca ttgatcatga cccggcaaat ccaaattctc ctttagaaaa
     5341 cagcattacc tgtctttatg ccgattttca aaataaaatt tggatcggaa ctctaaacag
     5401 tggcatgagt gctatgcgaa cagacaatcg ttcttttact cattacacca atctgggcaa
     5461 gtatgcaaac aactccgttt tcggaatcaa taaggatctt gatggaagta ttattgccgg
     5521 aactatagga ggaggtttga taatttttaa cagcgatgct tcaacttatt cttcatttac
     5581 acatagtaag acagatccat tttctatcag cagcaatagc atttggacaa tatatcgtga
     5641 taaggcaggc ataatctgga ttggaacaga caatggtctt gattattaca aggaagacct
     5701 gatgcgtttt aaaacagagt tgccactgcc ggatgatcca ttgagtaatg cgactcccaa
     5761 taagaatgtt ttttgttttc tgcctgaggg gaataatttg tggatgggtg ttttgggaac
     5821 aggtgtggtt gttaaagaat caacaggcac aatcaaacaa gtatataatg ctgcaaacgg
     5881 acttgcagac aacagtgtgt tctgtttaat caaagataga aatggtattg tttgggttgg
     5941 aacttataat ggtttaacgt ccatcaaccc acagtcagga gttgtgcgta gctatcggat
     6001 agcagacggt ttgaccaata gtaatatcag atcgcttgcc gaagatgaaa atggaaatat
     6061 ttatattggc acctacggtg gtgggctttg cgtacttaat agacaaacgg ataagatttc
     6121 aagtatccgt aaagaagatg gcaaaggttt aacgaataat ataataacgg ctttaaaata
     6181 tgatggcaat ggaaatttgt ttattggcac ctatggtggt ggattatgtt tgttgaacat
     6241 ttctgcaaat accataacag catttgaatc aaagcccgga agcaatacca caattagcag
     6301 taattatgtc aactcaatat acagtttttc aaatcagcaa ttgcttgttg gcacatacag
     6361 tggtggaata aatattttta attatgccac aaaaagtttt actgcattga ctgaaaccaa
     6421 tggtgttccc aataataatg tgacgaatat tgtttcagat gcagaaaaaa atatctggtt
     6481 tactacagga aatcaaatct gcaaaatgaa actggctcct tcagattcta ttctcaccat
     6541 gagaatttat gatgagcaag atggtgtttt taatcgcttc aatccgggtg catttgcaca
     6601 aactgcagat ggcaaaattt actttggggg catcagtgga attaatcgtt tcttgccatc
     6661 agctattcgc gataatcctt acatacctcc tgtagttatc acaaaatttt atctttttga
     6721 aaaaccttat ttcatggaca cgattatcat gtccaaaaat atgatagagt taacttacaa
     6781 tcaaaacttt ttttcatttg aatatgcagc tttaaattac attctaccgg aaaaaaatca
     6841 atatgcctac atgcttgaag gacttgaaaa gacgtggaca tattgtggca gtaaacgcga
     6901 tcgccaatat acagatcttg accccggcac gtataagttc agagttaaag catgtaacaa
     6961 cgatggaatc tggaatcagg atggaacata tattatcatc atcatcaaac caccgttttg
     7021 gaaaacctgg tggttctata ttttatccgg tcttactttc gtaggtttaa ccataactta
     7081 cattcgttgg cgtacacgaa cgctgatgaa acaatatgaa attttggaac agaaagtaca
     7141 agagcgcaca gcagaattgc gagaagaaaa agaaaaatca gaacagaagt cattagaaat
     7201 tgagcgcgca ttaaaggaac ttaaagacac acaatcgcaa ctgatacatg cagagaaaat
     7261 ggcttcttta ggtcaactta ctgcaggtat tgctcatgaa attcaaaatc cacttaactt
     7321 cgttaacaat ttttcggagt tatctcaaga tcttattgat gagttgaatg ctactgatga
     7381 caaagaagaa tggaagtcta tttctcatga tataaaaacc aatctcgaaa aaattaatga
     7441 gcatggtaaa cgtgcagagc gtatagtgaa gagtatgctc atgcacagcc gttcgcaaac
     7501 agcagagaaa gttttaacag acatcaacaa acttgcagag gatgtaatga accttgccta
     7561 tacaagcctt cgagcaaacg atagtaattt tgggtgtaag aatatgattg agttggatgc
     7621 ctcattgcca aagctcagca tagttcagca ggatatcagt cgcgtgttac tcaatatttt
     7681 taacaatgca ttttatgctg taaatcaact acgtaaaaaa tctgataaag catttcatcc
     7741 tgaagttcga cttagaacat ggaaagctgc aaacaaagtt tatattagta tcagtgataa
     7801 tggtggtgga attcctgatg atatcgttga taaaatcttc caaccattct ttacaactaa
     7861 gcctaccggt gaaggaactg gattgggttt atcattaagt tatgatatca tcaccaaagg
     7921 tcattatggt atgctaagtg tgaatgtgca gaaaggtata gggtctgagt ttgttattga
     7981 attaccccaa ctataataaa aatatcatgc aaacaaaaat tctgatagta gatgatgaac
     8041 gtgatgttga actgttgttc aggctaaaat tcagacgtga aatcaaagag aaacatattg
     8101 atcttcactt tgcatttagt ggtgaagacg ctttaaacta tcttcacaac attaacccta
     8161 tggatgtttt attggtattg tcggatataa atatgccggg catgaacgga cttcagttgg
     8221 ttgaaaaagt aaaggctgaa tttcctgcaa tgcgaattat tatggtgtca gcttatggtg
     8281 atgataagaa ttataatgaa gctaaggcga aaggtgtaga tgattttgta actaagcctg
     8341 ttgactttga agatcttaaa agaaaaatgg caagagtgat aacaatttag ttatggcaaa
     8401 aaaaatattg gtagttgatg atgagcctga tcttgaactt ttagtcaagc aacggtttcg
     8461 ttcgcagatt aggaacaatg aattgcaatt tgattttgca cacaatggtg aagaagcatt
     8521 acaaaagctg aaagatgatg ttgatattga tttgattttt acagacatca acatgcctgt
     8581 aatggatgga ttgaccttac tgaatcaaat taaagaaaac aagattcaac ctaaagcagt
     8641 tgtgatttca gcttatggcg atatggacaa tatccgtaaa gcgatgaaca gtggagcatt
     8701 tgattttatt accaaaccta tagacatgac cgaccttgac actactttac gtaaaggttt
     8761 gagcgaaatt gaaataatta aaaaaggtca aaaggcacag gaggcattac aacaaactat
     8821 tattgagaag gaaattgcag tactggaaaa agacaaggcc gaagaatcaa aaagactcaa
     8881 gcagcagttt cttgcaaaca tgagtcatga aatcaggacg ccaatgaatg caattatagg
     8941 aacggtaaat ctgttacata aatcaaacct tgatgataaa cagtcaaagt atattggcat
     9001 cataaaaaca agtgccgaca atcttttggt catcatcaac gatattcttg atatttcaaa
     9061 aattgaatcg ggcaagttta ccattgagga aattccgttt aacctacatg aaaaggtgaa
     9121 ccatgtttat gagtcgctta aagtgaaagc tgaagaaaaa ggtcttgcct ttaatgtgga
     9181 ttattgttca acactttctg ataattatat tggtgaccct gtgcggataa cacaggtgtt
     9241 gattaatctt acaggtaatg ctattaagtt taccgaaaaa ggatctgtaa caattagagt
     9301 tgcttcaata gctgatgttc ctaacggtgt cagcattagc gtagttgata ccggtattgg
     9361 tattgctcaa gataaaatac agcacatttt tgaaagtttt acacaggaaa gctctgatac
     9421 aacaagaaaa tttggtggta ccggccttgg gcttgctatc agcaaacaac tgatagagtt
     9481 gatgcatggt gaattagttg ttgaaagtac gcaaggaaaa ggttcttctt ttattatgaa
     9541 attgccattg gtagcaacaa atgaattggc aactgaaaca aaagacaagg taagttcaag
     9601 tgtagaaatc cgtaaaggat taaggattct gcttgctgaa gacaatcagt ttaatcagat
     9661 ggtagcagaa gatactttac gtatggttat tcctgatctt acattggata ttgttgataa
     9721 tggtgtgcaa gctatagaaa aactcacgtc aggtaattat gatttagtaa ttctcgacat
     9781 tcacatgcct gaaatggatg gctatgaaac aactcagttt attcgtacgc aaatgccgtc
     9841 accgttaaat ggtgtgccta taatggcaat gacagctaat gtaatatcag aagaaattga
     9901 caaatgtttt gcagtaggaa tgaatgccta tgtgagcaaa ccgtttaccc atgaagattt
     9961 atttgataaa ataaaatcgc tcacaagtaa gtagttttac agcagtcaat gttttttttg
    10021 actacaataa aatgattgct gctttttatt caacagcaat gccaaatggc attcttgcct
    10081 gaattttgtc ttgttctgaa atttgcttga gatatttcat cagcttcatg ctttctttca
    10141 tgtagtcggg catcatcata ctttcagatt cacctttcat atcttcaagt atttcctgta
    10201 ttggatcttt ttgttcagga agttctttta ttctgtagtt atccaatccg gctttttttg
    10261 ctgcaagagt aacagcatca ttcaatccgc cataaatatc aaccaaacca attctctttg
    10321 catcggtagc actccacaca cgtccttgtc ctatactatc caccataacc gtttccattt
    10381 ttctaccttc ggcaacacgt cctataaaag tgctgtaaac atcttcaaca ctgcgctgaa
    10441 gaatgtttct ctcagcaggc tcaagtggtc ttgttggcat accaaggtcg gcataaggtc
    10501 cggttttata agtatcaaat gttaatccta atttgttgtt gagcagtttt tgtgcattaa
    10561 ataataatcc aaaaacacca atagagccag taatggtggt aggttgcgca acaatggtat
    10621 cggccaaaca actgatgtag tagccacctg atgccgcaag gttacccata cttacaataa
    10681 atggtttttt ctccttagct aatttagttt ctcgccatat cacatcagag gccaatgcac
    10741 tgcctccggg agaattaact cttaaaacaa ttgcctttac tttatcatct aatcgagctt
    10801 tttttattgc ttctgctatt ctatcagaac ctacttggtc ggcttcgcca ctaccgctta
    10861 caatgtcacc cactgcataa actacggcaa tgcggtcagt ggtgaatttt ttatccgact
    10921 taacaacagc ttcattgtat ttgttcaggc tgatgtagtt gatatcatca tcattcacac
    10981 ctgatttttc tgcaagaatt ttttccaact catctttata acataattga tcaactaact
    11041 ttaacctaag tgcatctttt gcattttctg cagataaact gtcagcaatt tgttctaatt
    11101 cttcgcgact tattttgcgc gattctgcaa tttcactact tatagtttcc catatagagt
    11161 tgataaaagt ggcaacctgc tctttgtttt ccggactcat tttatccaaa ataaatggct
    11221 caatagcact tttaaatttt ccatgtctga taatttcagg ttcaatttca agtttatcca
    11281 atgcaccttt cataaacatc atctgtgtaa aaaggccttt aaaatcaaca gcaccttcag
    11341 gatgtacata aattttatcg gcaacgcttg ccagataata agctttggtt gaataatatt
    11401 cagcataggc aaaaataaac ttacctgatt ttttaaattt cactaatgca tttctgattt
    11461 ctgttgtagt agcatagccg gcttcaaaat aattcagatt aaggagaata cctttaatgt
    11521 tatcatcctt ttcagctttt tctatattgg caacaatatc atttagtcct ggttttttac
    11581 ttgccttcaa cgtgcttaaa ttcagattgc gaaatgggtt gttgtcgcta cgttctgcaa
    11641 tgggcatttt gaattctatt tttaatacag agttgctttt aacttctaca acttcttttt
    11701 taaaggcact gccaagcata acagcaaata aaaagaatgt taaaagacag aaaagaatca
    11761 ggcctgtgag tgtggccaaa acatatttca gaaattgttt cattttaaat ggacttgtgt
    11821 gagtttgaaa atttagcttt gcaaagctat aaaaatcaaa atggctttgc atcagaatac
    11881 tgcaattata cttgcaggca gtaatttagg taacagacta cataatctga ctgaggcagc
    11941 attgtttctt caaaggttgt caacaaaaaa aatggcatca tcatcagtgt atgaatcagc
    12001 accctggggt aatcagaacc agccgccttt tctgaatcag gcattcagca tttataatgc
    12061 atttactgca cacgaattgc ttgatgtttt attgaccttt gaaaaatctc agggacgaac
    12121 acgcaagcat cagtgggaac cacgcagcat agatcttgac atactttttt tcaataatga
    12181 aattatctgt acaccggaat tgacagttcc acataaacat ttgcatgaaa ggcgttttgt
    12241 gctcgagcct cttgcagaac ttatttctga ttttattcat cctgttttta aaaaaagtat
    12301 ttctgaattg ctagttgaat gcaaagatga tttgaaagtc tctaaatatc ctttatatgt
    12361 acagaaatag atttttaact attgaaggaa acattggggc cggcaaaact tcattagcca
    12421 agatgattgc cgaagagttt aattatcatc ttatattaga agagttcgca gaaaatactt
    12481 ttttacagca gttttatgat agtcctgaaa aatttgcctt cccgcttgaa atgtcattta
    12541 tggctgcgcg gtataagcaa ttgaaaacat ttttcaacaa aaacacactg ccttttccta
    12601 ttgtgtctga ttatatgttt caaaaaacac tgctatttgc caagtcgaat cttaaaaaaa
    12661 atgaatatgg gctttttgaa cagttttttc agatgctcaa tgtaggattg cctctgccgc
    12721 aattaattgt gtatttgcaa aaggaaatac cttcgctcca aaaaaatatt aaaaaacgtg
    12781 ggcgtgagta cgagcagaat atgaaggacg attatctact caaaatcaat aatgcatata
    12841 gtcgttttat gaaaaagtcc aacacagctt ccaatatcct aataatcaat acggaaggac
    12901 ttgattttgt gcgaaaaaga agcgattttc aaaaaattgt agaaactata gaaaagacag
    12961 gttttaggtt ctgatttaca agcactgtca ataaatctgt ttatttactt catatttaat
    13021 tttattattt aaaaccattg tgttatactt gcaccacaaa attttgtaaa atattaaacc
    13081 actctatacg atgaaagtaa aaaccttact cgtttcagct tttttaatct ctgctacgca
    13141 atttgcgttc gctcaggaat cggccagata cggccttcct aagaacaagg attttgacaa
    13201 atggtcagca ggactaactg taggattaaa ctggtttcag ggcgacctca acgaaaaaac
    13261 cggaaacaat aacagtatcc tgaataacat ccaagaccct gtattcggac ttaaagtagg
    13321 ctatcagata agccactctg tgcaggtaaa cgttcgtggt aattacacaa acttgagcgg
    13381 aaaaaagaac aagacaactt tccgtgttcc aaatgctgtt accagcccgc aaattaccag
    13441 gaacattaag tttaagtcac ctatatatca ggcaagtctt aacttaaatt ataccatagg
    13501 taacatcagc tttttacaac gtaacaagcg tttgcacttt tatggtgaaa ttggtttggg
    13561 tatatttagc tatgctccaa aagttactga tcttgacaat ggaactgttt atgtaaagaa
    13621 aggctctgta gctgaaggat ttttaccttt aagtttagga tttaaatatc agatcaagcg
    13681 ttttgatgtt ggtttacttg ccacatttaa caaaactctt aacgataaag tagatcaggt
    13741 atatgactct aaaacagaga gtgacaactg gtcgtttttc ctgttaggtg ttaactatac
    13801 attcggcaaa aaacaagcaa tgatggaatg ggttaaccca atggaagtag tttataatga
    13861 tctttctgat atgaaagaca gaatagatgt aatgtctggt gataaagata aagacggtgt
    13921 ttctgatttg ttcgacaaag acaactcaac tcctgaaggc gttaaagttt atggtgatgg
    13981 tactgcctta gatagcgatg gtgatggtgt tcctgataat gtggactctg acccttattc
    14041 aaataaagga gctaaagttg atgccagtgg tttagaggtt gatactgatg gcgatggtgt
    14101 tcctgacagt cgtgatatgg aaccaaacac tcctaaagga aacttagtaa acttccaggg
    14161 tatgactatt gcacctgccg gatcttatgg aaatgatggt atcacttcta acagcgctac
    14221 aggttggtta ccttcaatat tctttgatgt tgaccagtca gctatcaaat ctgtttacaa
    14281 cgaccgctta aatacagttg cacgtgtgat gataaaaaat cctaagctga aactgagtgt
    14341 tgttggtaat tgcgatgttg atgcctctga acaatacaat gataaattag gtatgaaacg
    14401 tgcagaagct gctataaatc accttgtaaa agtttatggt attgatgcct caagacttac
    14461 tgccacatca ttgggtaaga aagaacaact tgccaaaggc aacaagcgca tgaacagaag
    14521 ggttgatttt gaagtagcaa aataacctga ataatattta aaggaaaggc cggactttag
    14581 tccggctttt tttatgcttt cattttctga aacaggtcat atatcattat gcctgcacac
    14641 actgcaatgt tcaaggaatg tttggtgcca tgttgcggca gttctattgc cccatcacac
    14701 attgctattg tttcattgct cacaccatca acttcattac caaaaacaac agctaacttt
    14761 tcatcttttt caggtttaaa atcaggcata gctatacttt gacttgtttg ctctgctgca
    14821 aaaattttat aaccatttgc tttaagttta gaaatacaga ctttaatatc agaataatat
    14881 tgccagttta ctgactctgt agcgcccaat gcagttttta aaatatcttt gtgtggtggt
    14941 tgtgccgtga tgcccgttag aataattttt tctaccggaa aggcatcaca ggttctgaaa
    15001 acagagcctg tattaagtgc acttcttata ttttcaagaa taatgacaac aggaattttt
    15061 tgtatttgct taaacccttc tgtcgaagtg cgacccattt cgctgccggt taactttttc
    15121 attttttaca attgaagaca aaagaaaaaa ataaaagcga aactccgctc atgcagcaat
    15181 acaatgccat aaaggcaaag tatcctgatg cattattact ctttcgtgta ggcgattttt
    15241 acgaaacttt tagtgaagat gctgtaaaga catcaaaaat tctgggtatt gtcctcacca
    15301 aacgttccaa tggtgcagca tcagaaatgg agcttgcagg cttcccctat catgcgctcg
    15361 atacctattt gcctaaattg gttcgtgccg ggcagcgtgt tgccatctgc gatcagttag
    15421 aagatccaaa gacaacaaag accattgtta aaagaggcgt aacagaattg gtttctcccg
    15481 gtgttaccta caatgaaaaa attcttgaaa acacacacaa taattttctt gcagtagttc
    15541 atcttacaga agatgtttca ggtgtagctt ttgcagatgt ttcgacagga acatttttta
    15601 ctgcacgtgg taaagatgca tatatagaaa agctgcttca gaattttaaa cccagcgaag
    15661 ttgtgttgca gaaatccatg caaaaagatt ttattgcacg ctttggcgat ggcttctata
    15721 tctacacact cgatcaatgg gcttttcaga aagaatatgc agaagaagtt ttactcaaac
    15781 actttcagac caattcactt aaaggtttcg gtattcacga aatggacgaa atgattgtgg
    15841 catgtggtgt tgccttgcat tatttcggac aaatgcagca gcaaacgctg tcgcacatca
    15901 caggcattgc acgcattgac gaagaacaat atgtctggct cgataaattt acgatccgca
    15961 atcttgaact tgttgcctcg ccacatgaaa atgcacgcac cttagttgat gttattgaca
    16021 gaactgtcac acccatgggg tcgcggctac tcaaacgatg gatactccta cctctaaaag
    16081 acattcaaac cattcatgag aggcacgatg ctgtagccgc tttcacctta gatgaaacat
    16141 taagagggca gatagcaaca tctctgcgtc atgttggcga tatagaaaga atgctttcta
    16201 aaattgcact ccgcagaatt tcaccacgcg aaatggtgca actcaaacgt gcattatctc
    16261 agattcaacc tgtaaaagac aaccttgcta ctgccgggaa cagtacgttt aatgttttgt
    16321 cagaacagct aaatccctgt aacctactca ttcaaaaaat tcaggatgcg ctaattgaag
    16381 atgcaccacc acttgcatca aagggaggtt tcattgcaaa aggattcagt cagcagcttg
    16441 acgacttaag acaagtagca ttttcaggca aagattatct gctgaaaata caacagcgtg
    16501 aagttgaaca aaccggaatt tcctctctca aaattgcctt caacaatgtg tttggctatt
    16561 acattgaagt caccaatgca cataaaagca aggtgcccga aagctggata agaaaacaaa
    16621 ccctcgtaaa tgccgagcgc tacatcactc ccgaactcaa agaatacgaa gacaaaattc
    16681 tgggtgctga agaaaaaatt tcaaccatag aaaatgaaat ttttcaggag ctcatcagct
    16741 ttacacaaaa ctacattcag cccttgcagg tcaatgcatc tgttttgtcg cagctcgact
    16801 gcctattgtc ttttgcaaac gttgccatcg aaaataatta cacacgccct gttgtaaacg
    16861 acagccttgt tctttctatc acagaaggcc gccatcctgt gctcgaaaaa caaatgcctc
    16921 cgggcgaagc ctatattcca aactctattt ttttagacca gcaaacgcag cagattatca
    16981 tgatcaccgg ccccaacatg tccggtaagt cggccttgct caggcaaacg gcactcattg
    17041 tactcatggc gcaaacgggc agttttgtcc ctgccaagag tgcagaaatt ggctgggttg
    17101 acaaactctt taccagagta ggagcaagcg acaacatctc ttctggcgaa tcaaccttta
    17161 tggtcgaaat gaatgaaact gccagcatcc tcaacaacat tacacagcgt agcttggtgc
    17221 tgttagacga aattggtcgc ggcacaagca cctatgatgg catttctatt gcatggtcca
    17281 ttgccgacta tatccacgaa tccaaaaatg cacgtgccaa aacactgttt gccacacatt
    17341 atcacgagct caatgaaatg accaactcct acaacaggat taaaaactac aatgtggcaa
    17401 taaaagaagc aggcaataaa atacttttcc tgcgcaaatt agtgccaggt ggcagcgaac
    17461 atagttttgg tattcacgta gcacgaatgg caggtatgcc gccacatgtc atcaacaagg
    17521 ccgaaaaaat gctagccctt ctcgaaaaag cacacagcaa cacagaattg ctgcgcaatg
    17581 ccggacatca ggccgacaaa gacgccatgc agctaagctt cttccagctt gacgaccctt
    17641 tattggaaca aataagagac gaaatactca aaaccgatgt caacaccctc acacccgtag
    17701 aggcgctctt taaactcaac gagataaaaa cgctgctttc aaaaaataaa aagtaaaaaa
    17761 tttgttctta ttttattttt tataattttg caatcccttc taacgaagtc gggaatttaa
    17821 gtattgaaat atcaagcaat tatgcggaag tagctcagtt ggtagagcgc gaccttgcca
    17881 aggtcgaggt cgcgggttcg aaccccgtct tccgctctca acaaaagtta ccgccacaca
    17941 tgtagctaac ggttggcctc tgttaaacct gtagctgttt attttcggta actttttttt
    18001 tgaagttcct ttttatccgg atggtggaac tggtagacac gcaggactta aaatcctgtg
    18061 gaccttaaac gtccgtgtgg gttcgattcc cactccgggt actttttttc aaagtaattt
    18121 ttttaaaaac atttgtgtgg gttctcctcc gcctgcggtg gatgtggcgg accactccgg
    18181 gtactttttt tcaaagtaat tttttttaaa aacaattatg ttatatatca taaagtagct
    18241 caccattccg tgtactatta aaaaactaat aataatttta aa
//
LOCUS       DAORQT010000010        18191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_250_length_18191_cov_11.182289, whole genome shotgun sequence.
ACCESSION   DAORQT010000010 DAORQT010000000
VERSION     DAORQT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_18191_cov_11.182289"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PK134_00845"
     CDS             <1..909
                     /locus_tag="PK134_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB37431.1"
                     /translation="RIALAYKPRWKRDFNFRLSGGFYYQPPFYKELHNPDGSLNTSLK
                     SQQSIHVVGGTDYYFKAWGRDFKFVTEAYYKWLNDLIPYKVDNVRIRYLGKNNSKGYA
                     TGIDFRINGQFVNEAESWASLSLLKTEEDIQDDVYYIRLNARGDTIRGYTIDKKAVDS
                     IQVIPGYLPRLTDQRLTFSIFFSDYLPKFPTFKMHMTLVFGTGFPVGPPGNNRYTDIN
                     RYPFYRRVDIGFSKIFIDENRPAKSRFAAFNKLKALSLSIEVFNLFQVNNVVSYTWVS
                     DVYGTKYGVPNYLTGRQLSLRLSAKF"
     gene            1052..1501
                     /locus_tag="PK134_00850"
     CDS             1052..1501
                     /locus_tag="PK134_00850"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRB37432.1"
                     /translation="MRNHSESRFLTERQSRIGEFLFTLKVVREFIKGFRTLHFVGPCV
                     AVFGSARFKEEHHYYQLGREVGANVSRLGFTVMTGGGPGVMEAANRGAKEAGGFSVGC
                     NIILPREQQPNKYLDKMVEFHHFFCQKSFDVQILLCFCGFAWRCWHN"
     gene            1410..1772
                     /locus_tag="PK134_00855"
     CDS             1410..1772
                     /locus_tag="PK134_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRB37433.1"
                     /translation="MNFIIFFVRKVLMFKYSYAFVVLPGGVGTIDEMFEALTLIQTKK
                     VSDFPVVLMGKNYWQPVIDQLKVFDEHKTALHSELDSLLITDSLDEMTEYLRVKAIER
                     FKLSKAMKFNRSKWFLEE"
     gene            1868..2872
                     /locus_tag="PK134_00860"
     CDS             1868..2872
                     /locus_tag="PK134_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRB37434.1"
                     /translation="MKVFRLCFFIFISSMLQAQDIHFSQFYNSPLTLNAANTGLYEGD
                     YRFANTYRSQWSKLDPGYRTNAFSFDKTLYTGADQISGGFLFIYDRSGINRLQSIRLG
                     LSAAYHRTFGVHSLSAGFQWNYIFKSIDRSKITMPEQFNMNTGYFDPSMPTGEMLLDE
                     KRSFGDINAGLVYQVELNKHFIQAGASFFHLNKPGDTFFNSSNKLPVRTCLTVSDKIG
                     IGNDYQLHTRLLMMQQVKANEYVAGVTLVKQLNNGESKFTEVNGGLYVRTSPGNGTDA
                     VIPVIGIKYDLFDVALSYDINISTLHTATASRGGFEISLIYTALSSRLFKIKIPCERY
                     "
     gene            2859..4946
                     /locus_tag="PK134_00865"
     CDS             2859..4946
                     /locus_tag="PK134_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB37435.1"
                     /translation="MNAIKTRAILLLILLISSVHLYAQQNDELKKYGTAQLKRMGSYA
                     AKINDFSSSAMYYEEYCKRKPTNEKVWFLYAESLRKSKDYRKAEDAYMKAYNLNPKLN
                     ALALYHYANVQKSLGNYKKAEEYFTKFKKEYKGSDKSVYTKLIKNSGDAARFADSLMH
                     SPSKVAIEHLPEDINSSHVESSPFYINDSTIIYGSLKTDQVTFDFNPRDSSADEPFRK
                     IYVAVRDSEIWKDDGELPGFYNSNSAHTLNGVYSQDKSRFYFTRCKRNRQNKVICAIY
                     VSFLNNGIWTEPVSLGTEVNDPKYTSTQPAIGLNTQKKGEEIIYFVSDREGGKGGYDI
                     WYTSFDNRSKTYKTPVNLGTKINTSGDEVTPYVDAQTQTFYFSSDGWQGLGGLDIFKS
                     IGGMKKWTHPVNLGYPVNSNYDDLYYVLSPSNKDIGMIVSNRNVTDNGMNKGPCCDDI
                     FEFNILDAVRLNVKGFLYETDSTQKGKKLAMMPVRRARITLEMKNAEDSTFIPVSTSN
                     TDETGHFNLIVSPRQEYRITARKEGYLYESVALSTMNKTRSGDISIDIHIRSIPETPL
                     LLTNIYYEFASPLLTAKAMAALDTTLVIFMQRNVDLGVEIRSHTDAIGSDDANMTLSQ
                     KRAESVVNYLVAKGINANRLRAKGFGETMPVAPNTKPDGRDNPKGRQLNRRTEFKILG
                     KMRDGVIREETDF"
     gene            complement(4949..5533)
                     /locus_tag="PK134_00870"
     CDS             complement(4949..5533)
                     /locus_tag="PK134_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HRB37436.1"
                     /translation="MIYEFNGFRPVVDSSSFVHEQATVTGDVIIGKQVYIAPGAAIRG
                     DWGTIIIEDGCNVQENCTIHMFPGVTVILKQGAHIGHGAIVHGATIGKNSLIGMNAVI
                     MDNAVIGDGCIVGALCFVPDGMIVPDRKIVVGNPAKIIKDVSDEMLHWKTKGTELYQQ
                     LPHELTATLKPCAPLRSIEPNRKKHDAVYTTWKK"
     gene            5668..10101
                     /locus_tag="PK134_00875"
     CDS             5668..10101
                     /locus_tag="PK134_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37437.1"
                     /translation="MSKYFASIILVFIFLTSTLRAQTLKSFNTDPSSYLLDLKSFFEE
                     TNKKEAEKIMEEFKPVFLGKFNAQQQQTIITTSNLMLKKRMKAFPDFVTYTSVLNAFA
                     SSGQEAATFTSWNSSFEKAIAKLSVRKLGDFLEISQLLFRNNTLYESSAVAWSASNNK
                     FSFGFDSLPKVTFSGMTLRCLGKGDSSVIEGTKGVYYPNNLLFFGDGGTVNFTRAGIS
                     VSEANAVVKRYAINLKGSEYSMDSVAFTYKKYFDQELKGRYIDKLLANVNDSNATYPR
                     FYSYAANLDIKNMVKDADYKGGFSLQGSKMVGSGNRRQDASITFNLNGKPQLKLLSQG
                     FIFRTDRIVSVNAAAVIYWEKDSIYHPGVEFKYIYGDKTVTLTKNGQTAINSPYFDSY
                     HKMDLDFDQLVWKITDPLMDLKMISGGGESKLKFESVNFFSRQRFDKIQGLSEVHPLF
                     KIKQYAEEHNVKVISVPEFAEYLKLTENTVRNQILQLSSLGFISYDADADKFIVKDKV
                     MYYLNARTGKVDYDELKIESMISGLSNATINLLNFDINMRGVSHVLISDSQEVYIQPL
                     DQEMTLKKNRDIVFNGRVHAGRLDFYGKEFTFNYADFNIDLKNVDSLRFKVPADSVDE
                     RGKKRLIPIKSILQQLNGKLSIDNPTNKSGFVPNEEYPVFVCTNKSYIYYDYPEIFGG
                     VYKRDKFYYHLDPFSIDSLNTLTKEGLVFEGSMVSADIFPEWRQQLTVQRDYSLGFIT
                     NTPDAGFAAYSGKGKYRDQISLSHEGFLGKGEVDYLSSTSKSNNIIFFPDSMHAVTQS
                     FAIRKEQVGATRFPDVKNEDVFVNWRPKSDKMFVFKRSKDFALFNGEAVLDGNLVLMP
                     SGIKANGIIRYQQSQLISNNHFIKEHSYGSDSAEFKLNTDAGDLLALHTQNVKANIDL
                     DKRIGDFSSQGEKAKVEFPVNEYISYIRDFKWYIDKSELEFGGSDATADKANEFVSVN
                     RQQDSLRWDAPLATYNLKSYLLKGFKVKEILVADASIIPDLGNVVVERNAVMRPLVNS
                     KVIANTTSHYHTMLNTNITIQSRMNYSGDGNYEYMDQTKVKHKIKLTQIGVDTSKQTF
                     AIGEIPDSSRFFISPTIQYKGKLRINAPKENLYFNGFAIVNHHCNTFERNWFGFAGDI
                     DPKGVNIPIQSPANESLQKLYSGIYFGSDSTMMYPAFLSMKKHSSDAEVISAEGYLTF
                     DNNLQQYKISGTIPGTDSTKSASVITNTVILDDKRCQLIGEGRINLTQDFGQLSITNF
                     GMVNYSTVNDSIGFDAVMATDFMFNDDALKQMAEMFNSIPALQPFMDDRPAYRNALTN
                     MIGKNKAEKLLNETGLYGSARRIPSELQHTLMLSELKFYYNKERASYISVGNIGLGVI
                     GKTYVGRMVKGKVEIMRRRSGDVLNIYLEALPDMWYYFNYQRGVLQAISSDEKFNKPI
                     DEMKPEKRIASEKGGLEPYQFMLATERKKNEFLKRIKDADL"
     gene            10157..10630
                     /locus_tag="PK134_00880"
     CDS             10157..10630
                     /locus_tag="PK134_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRB37438.1"
                     /translation="MQREHHYQANIIWTGNKGSGTDSYLGYARSYTVNIDNKPEIQGS
                     ADAAFHGDKTKYNPEEMLLIALASCHMLWYLHLCSEAGVVVVEYTDRATGLMTETPSG
                     NGKFESVTLNPIVVIRDQTKNEIAIELHKKANEFCFIANSVNFKVNHKPVFKLMK"
     gene            10782..11750
                     /locus_tag="PK134_00885"
     CDS             10782..11750
                     /locus_tag="PK134_00885"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HRB37439.1"
                     /translation="MKNTFFTVAFILCAFFVKAQSSNVILFTENGEKFFAILNGVRQN
                     TSPETNVKVTGLNANFYKLKVIFEDTKLGEKDFNLAFNPGMETTFSIKFIAKKNSYVL
                     RPLSEVPLAQALPPAANQTVVAYSEVPAPPPAATTVTHTTTTTTTDGTPMPGDNVNVN
                     IGVNMNEAGGGINMNVSGGTTQTTTHTTTTTVTHTTTSEPTAPPIAPPPPAPVVYLPG
                     YNGPYGCPVPMAPQDFSELKQSISSKSFEDSKMTIAKQVLRNNCLFTSQVKDLMGLFT
                     FEESKLDFAKYAYDYTYDIGNYFKVNDMFTFESSIDDLNNYINSKH"
     gene            complement(11816..12262)
                     /locus_tag="PK134_00890"
     CDS             complement(11816..12262)
                     /locus_tag="PK134_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB37440.1"
                     /translation="MVIREAKIDDIKQIQRVRNSVKENTLSNPDLVTDKDCAEYISER
                     GKGWVCEIENQIVGFAIADLKENNIWALFLDPKFEKMGIGRHLHKTMLDWYFSQTKQT
                     VWLGTAFNTRAEQFYRKAGWTEVGTHRSKEIKFEMTYSDWIKIPNH"
     gene            complement(12347..14182)
                     /locus_tag="PK134_00895"
     CDS             complement(12347..14182)
                     /locus_tag="PK134_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRB37441.1"
                     /translation="MRLIRNFLVLSIFWMLFFGIFRIIFFLFLQYNAANIQLNELPST
                     LFYGIRLDISATAYISALPLLLMLIHALTENTFIKKLFLVYNGIILFVLILLHVSNLF
                     LYRSWGVLINYRALSFLADWRQMFLSVNNFELIVGIICIILLFILTWFVFRKFTKHFF
                     DNKLAIIQSLLQFIVSAAMLVIMFRGGVQMLPVNESAAAFSENVTLNHIATNPAWHLG
                     HNLNQAKSENASYYKFYSDAVAEEHVKKLFQTPIDLMPVLNNKKPNIVIIMLESFTVD
                     VVGSMGGETGVSPTIDSLIHDGLSFKNIYSSGARTDQAFVAVLSGFPAQPNQSIMRFP
                     DKTAKLPSLPAELKKQGYSTSFYYGGDLDFSNLNTYLVNCGFEQITGKKKFKQQQLSA
                     KWGAHDEFVFEKNLNDLNQAKEPFFSVVMTLSSHEPYDIPIQSPFGKDNPNEQFKGSA
                     WYADHSLGNYLAAAKKEPWFKNTLFVIVADHGNKIPRDRSYYSHYIRHIPMVMAGDVV
                     KNIYKNKIVYSTGSHHDIPSTLLHQLGFNSDMFNWSNDLLNRKRNGFAYLCMDDAEGW
                     VTDSCTFVYDLSNQKFIYKRPYYAIADTVTAKSYLQQLYSTFSNL"
     gene            complement(14184..14924)
                     /locus_tag="PK134_00900"
     CDS             complement(14184..14924)
                     /locus_tag="PK134_00900"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRB37442.1"
                     /translation="MAKIINWFCFLLLLVFCSCQKDHMFDCFKGTGNDKSEIRNITGF
                     NRIKASNNVDVKIYPGYDYQVEVMAGSKLIEGITTEVKDSMLYIRNENACNWVRSFKN
                     KFTVKVWMPDIAELTVNGSGNIDLMDTIRHNEFTFNNWGASGKVTFLFNTGAVRTNIH
                     TGPGDFVFHGYVGVHYFYNNGNGIADASNLNTEVTFTENKGANKEYVRAKQWLTAKIS
                     YVGDIYYFGNPDSVVHEGTGNGKLIKAD"
     gene            complement(14908..15861)
                     /locus_tag="PK134_00905"
     CDS             complement(14908..15861)
                     /locus_tag="PK134_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRB37443.1"
                     /translation="MSELTIPVDLENPVAFFGINDSNLDLLRKKFPAFKLIVRGNELK
                     ILGDEKQIHWVAKKIIGAIEYFHHYGKFDEQVLEKLMADGQNSDTLVNPPSGNGSDVL
                     VFGPNGVVVKARTANQQKMVDSCNKNDIVFAIGPAGTGKTYTAVALAVRALKNKEIKR
                     IVLTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALFDMIPGEKLKTFIENGVIEVAP
                     LAFMRGRTLDHAFVILDEAQNTTESQLKMFLTRMGPAAKFIVTGDVTQIDLPNKQPSG
                     LIQAMKILKNIKGIDFITLDAGDVVRHRLVKDIILAYGKNH"
     gene            16035..16820
                     /locus_tag="PK134_00910"
     CDS             16035..16820
                     /locus_tag="PK134_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRB37444.1"
                     /translation="MSLITLTTDWGLTDHYVAALKGELLSMLPGVNIVDISHQIPAYD
                     FTKAAFVFKNAFYFFPPQTIHLICVDKQSKKTEDTHSGWMVIQYGESIIVCRNNGFFT
                     LVSKQEPLKSVYIEGSEEVSVAHEKSFLVTLLHDLVHHKPLEEIGQPIKSFYQSSMFQ
                     PSHDANTIQGTVIHIDTYGNAITNIEKEYFENVVGAKRFEIDMPRHGDAITKIYNRYD
                     DVTPGQLVALFNASGYLEIALNQGDISLLYGLEYGDKIRIFLQ"
     gene            16817..17125
                     /locus_tag="PK134_00915"
     CDS             16817..17125
                     /locus_tag="PK134_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRB37445.1"
                     /translation="MITRIVRLPVKPDEGKNFEQLFDTIKEKIAAFDGCLSVELHKDV
                     ASSDYFTYSLWQSESHLQAYLDSSFFTDVWPNAKKLLHQRANAWSLEKVLNVWKVAKF
                     "
     gene            complement(17473..>18191)
                     /locus_tag="PK134_00920"
     CDS             complement(17473..>18191)
                     /locus_tag="PK134_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB37446.1"
                     /translation="YYQINVGELKTTVYHYGLDYAESGEDEEGGAKRTILKTPYVWDT
                     IERYNEIYDSNQDTEETDINEGIDLLSIIKRGESNQVEFKPCLLYNFKTQAAGIGVKY
                     LIAKAICGFLNSNGGVLFIGVNDNGNIQGLENYDYSLFHGENEKDKLLLEFDSLLAYF
                     FDLSIKPLVDLSIKKVEGKDILVIVITESYKPIFLKNKKNEFIEKELYIRMNASTRQI
                     VNIEEMVEYVFNKQWKKPSR"
BASE COUNT         5807 a         3126 c         3388 g         5870 t
ORIGIN      
        1 cgtatagcac ttgcctataa accaagatgg aaacgcgatt ttaacttccg tctttcagga
       61 gggttttatt atcaaccgcc attctacaag gagttgcaca atcctgacgg aagtttaaat
      121 acatctttga aatcacaaca atctattcat gttgttggcg gaacagacta ttatttcaaa
      181 gcgtggggtc gcgattttaa atttgttaca gaagcttatt acaaatggtt gaatgacctt
      241 attccttaca aagttgataa tgttcgaata cggtatttgg gtaaaaataa ttccaaaggt
      301 tatgcaacag gaattgattt tcgtatcaat ggccagtttg ttaatgaagc agagtcatgg
      361 gcaagtttat cacttttaaa aacagaagaa gatattcagg atgatgttta ttatatcaga
      421 ttgaatgcac gtggcgatac catcagagga tacaccattg acaaaaaggc tgttgacagt
      481 attcaggtta tacccggata tttaccacga ttaaccgatc agcgacttac attcagcata
      541 tttttttctg actatcttcc aaaatttcca acatttaaaa tgcacatgac ccttgttttc
      601 ggaacaggat ttcctgtagg cccgcccgga aataatcgtt atacagacat taaccgttat
      661 cctttttaca gacgtgttga tattgggttc agtaaaatat ttattgacga aaacagaccg
      721 gcaaaaagtc gttttgctgc gttcaataaa ttgaaagcgc tttcactttc aattgaagtg
      781 tttaatcttt ttcaggttaa caatgttgtt tcctatactt gggtgagtga tgtttatggt
      841 actaaatatg gcgtacctaa ttatcttacc ggaagacaat taagtttacg actcagcgct
      901 aagttctgat tacatacgtc acaagattat tagcttttca cagtaaaaat aattttttga
      961 ttcctcatct ttaaatcata agctgatttg tttatatact cattgcgttt tgaagaatct
     1021 tttaaatttg aatttttaaa tttttcagct tatgcgtaat cattccgaaa gcaggttcct
     1081 taccgaacga cagtcgcgaa ttggcgaatt tctatttact ctcaaagtag taagagaatt
     1141 tataaaagga ttcagaacat tgcattttgt tggaccttgt gttgcagtgt ttggctcagc
     1201 acgatttaaa gaagagcatc actattatca gttaggccgt gaagtagggg caaatgtcag
     1261 cagattggga tttacagtga tgaccggtgg cggtcccggt gttatggaag cagctaacag
     1321 aggggcaaaa gaagcaggag gtttttctgt gggttgcaac atcattcttc ctcgcgaaca
     1381 gcaacccaat aagtatcttg acaaaatggt tgaatttcat catttttttt gtcagaaaag
     1441 ttttgatgtt caaatactcc tatgcttttg tggttttgcc tggaggtgtt ggcacaattg
     1501 acgaaatgtt tgaggcttta accttgattc aaacaaaaaa agtaagtgat ttccctgtag
     1561 tacttatggg taaaaattac tggcagcctg taattgatca gctcaaagta tttgatgagc
     1621 ataagacagc cttgcactct gagttagatt ctttgctgat tacagatagt ttagatgaaa
     1681 tgacagaata cctgcgcgtg aaggctattg agagatttaa gttgtctaaa gccatgaaat
     1741 tcaaccgctc gaaatggttt cttgaagaat gagcctgact gttggcaatt attcaataaa
     1801 aactttcgca attcatttct atcttatatt tttatcctgt tatttttcag agtatttaca
     1861 ggactaaatg aaagtattcc gcctttgctt ttttattttt atttcttcaa tgctgcaagc
     1921 gcaggatatt catttttcac aattctataa ttctcctctc acacttaacg ctgcaaatac
     1981 aggattgtat gaaggcgatt atcgttttgc aaatacttat agaagtcagt ggtcgaaact
     2041 tgacccgggt tacagaacca atgctttttc atttgacaaa acactttata ccggagcaga
     2101 tcagattagc ggaggctttt tgttcattta cgaccgttcg ggaattaatc gtttgcaaag
     2161 tattcgctta ggactatctg ctgcttatca tcgcacgttt ggagtacaca gtttaagtgc
     2221 agggtttcag tggaattata tttttaaatc tattgacaga agtaaaatta ccatgcctga
     2281 gcagtttaat atgaacaccg gttattttga tccatcaatg cctaccggtg aaatgttgct
     2341 tgacgagaaa cgttctttcg gtgatattaa tgcagggtta gtttatcagg ttgaattaaa
     2401 taaacatttt attcaggcag gtgcttcatt ttttcatctt aacaaaccgg gtgatacttt
     2461 ctttaacagc agtaataaat tacctgttcg gacatgttta accgtttctg ataagattgg
     2521 aattggtaat gattatcagt tacacactcg attattgatg atgcaacagg taaaagccaa
     2581 tgagtatgta gccggagtta cacttgtaaa acaacttaat aacggagaat caaagtttac
     2641 agaagttaat ggtggtttat atgtacgtac atcacccggt aacggcactg acgcagtaat
     2701 acctgtaatt ggcattaaat acgatttgtt tgatgtggca ctgagttatg acatcaatat
     2761 ttcaactctc catacagcaa ctgcaagccg gggtggattt gaaatatctc ttatttacac
     2821 tgcattaagt tcacgtttgt ttaaaataaa aataccctgt gaacgctatt aaaacaagag
     2881 ctatcctgct gctgatattg ttaatcagtt ctgttcattt atatgcacag cagaatgatg
     2941 aattaaaaaa atatggcaca gcacaattaa aacgaatggg tagttatgct gcaaagatta
     3001 acgatttcag cagttctgca atgtactatg aagagtattg taagcgcaaa cctactaatg
     3061 agaaggtttg gtttctttat gctgaaagtc ttcgtaaatc caaagattac cgaaaagctg
     3121 aagatgctta catgaaagca tacaatctga atccaaaact aaatgcttta gcgttgtatc
     3181 attatgccaa tgttcaaaaa tctcttggta attataaaaa ggctgaagag tattttacaa
     3241 aatttaaaaa agaatataaa ggctctgata aaagcgttta taccaaatta attaaaaata
     3301 gtggtgatgc tgccaggttt gcagatagtc ttatgcatag cccttcaaaa gttgctattg
     3361 aacatttgcc agaagatatc aattcatcac atgtagaatc atcgccattt tatattaacg
     3421 attcaacaat tatttatggt tctctgaaaa cagatcaggt aacttttgat tttaatccaa
     3481 gagattcgag tgctgatgag cctttccgaa aaatttatgt tgccgtgcgt gatagtgaga
     3541 tatggaagga tgatggtgaa ctgcccggat tttataattc aaacagtgct catacactca
     3601 atggtgttta ttcgcaggat aagagtagat tttattttac gcgatgcaaa agaaatcgtc
     3661 agaacaaagt tatatgtgcc atctatgtaa gcttcctgaa caatggaata tggacagaac
     3721 ctgtttcttt aggtactgaa gtaaacgatc caaaatatac ttcaacacaa cctgccattg
     3781 ggcttaacac gcagaaaaaa ggagaggaga taatttattt tgtcagtgac cgcgaaggtg
     3841 gaaaaggagg ctatgatatt tggtacactt cttttgataa cagaagtaaa acttacaaaa
     3901 cgccagtcaa tcttggaact aaaattaata cttcaggtga tgaagtaaca ccctatgttg
     3961 atgcacaaac acaaacattt tatttcagct ctgatggatg gcagggatta ggaggattag
     4021 atatttttaa gtcaattggt ggaatgaaaa agtggactca tcctgtcaat ttgggttatc
     4081 ctgttaattc caattatgat gatttgtatt atgtgctatc accaagtaat aaggacattg
     4141 gcatgatagt atcgaacaga aatgtaactg ataatggcat gaataaaggc ccttgttgtg
     4201 atgatatttt tgagtttaat attttagatg ctgtaagatt aaatgttaaa ggctttttat
     4261 acgaaacaga ttcaacacaa aaaggaaaga agttggcaat gatgcctgtt cgaagagcac
     4321 gtattacttt ggaaatgaaa aatgctgaag attctacttt tattcctgtc agcaccagta
     4381 atacagatga aacaggtcac ttcaacctta ttgtttctcc tagacaggaa taccgtatca
     4441 cagcacgcaa ggaaggttat ctttatgaat cagtagcgtt gagcactatg aataaaacac
     4501 gttccggtga tatttcaatt gatatacaca ttcgttcaat tcctgaaaca ccattactgc
     4561 ttacaaacat ttattacgaa tttgcaagcc ctttgctaac tgcaaaagct atggctgcat
     4621 tagatacaac cttggtgatt tttatgcaaa gaaatgttga ccttggtgtt gaaataaggt
     4681 cgcatacaga tgccattggc tctgatgatg ccaacatgac tttgtcgcaa aagcgggcag
     4741 aaagtgtggt gaattattta gtagcaaaag gaattaatgc caacaggttg cgtgcaaaag
     4801 gatttggtga aacaatgcct gtagcaccaa acacaaaacc tgacggaaga gataatccca
     4861 aaggcaggca gttgaacagg agaacagaat ttaaaattct gggtaaaatg cgtgatggtg
     4921 taatccgcga agaaaccgat ttctgatttt attttttcca ggtagtataa accgcatcgt
     4981 gctttttacg attgggttct atacttcgca aaggcgcaca gggttttaat gtagctgtta
     5041 attcatgcgg taattgttgg taaagttcag tcccttttgt tttccaatga agcatttcat
     5101 cgcttacatc ttttataatt tttgccggat tgccaacaac gattttcctg tcaggaacaa
     5161 tcatgccatc aggcacaaaa cataaagcgc caactataca accatcgcca attacagcat
     5221 tgtccattat tacagcattc attcctatca aagaattttt gccaatggtg gcaccatgaa
     5281 ctatggcacc atgaccaatg tgtgcacctt gttttaaaat aactgtaaca ccgggaaaca
     5341 tgtgaatggt gcagttctcc tgaacattgc agccatcttc aataatgatt gtgccccaat
     5401 cgccacgaat ggcagcaccc ggagcaatat aaacttgttt accaataatt acatcacctg
     5461 taactgttgc ctgttcatga acaaaactac tgctgtccac tacaggtctg aatccgttaa
     5521 attcgtaaat catttgaata actgatgttt cttgtaacga tgcaaatgta aagctaattg
     5581 atgtgccaaa actttaacaa ttgttggtaa ctatctgtaa gtattaatct gtcagaagtt
     5641 tatttttgtg ctaaaaccat cacattcatg tcaaaatatt ttgcttcaat cattcttgta
     5701 tttatttttc tgacttcaac attaagggct caaacactga agagttttaa taccgaccct
     5761 tcaagttatc tgcttgattt gaaatcattt tttgaagaga caaataagaa ggaagcagag
     5821 aaaattatgg aggaattcaa gcctgttttt cttggtaagt ttaatgcaca acaacaacag
     5881 actattatta ccacaagtaa tcttatgttg aagaagcgca tgaaggcttt tcctgatttt
     5941 gttacttata catcggtgtt gaatgctttt gcttccagcg gacaagaggc agctacattt
     6001 acatcatgga attcttcttt tgaaaaagca atagcaaaac ttagtgtaag gaaattaggt
     6061 gactttcttg aaatatctca attgcttttt cgtaacaaca ccttgtatga atcaagcgct
     6121 gttgcatgga gtgcaagcaa taacaaattt tcatttggtt tcgattcttt accgaaagtt
     6181 actttttccg gaatgacttt gcgatgtttg ggcaagggtg atagctctgt aatagaaggt
     6241 acaaaaggag tttattatcc caacaacctt ttattttttg gcgatggggg gacagtaaat
     6301 tttactcgtg caggaataag tgtttcagaa gcaaatgcag ttgtaaaacg ctatgctatc
     6361 aatttaaaag gttcagaata ctcaatggat tctgttgctt ttacatataa gaaatatttt
     6421 gatcaggagc tgaaaggccg ttacattgat aaattgttgg caaacgtaaa tgattccaat
     6481 gctacttatc cgaggtttta ttcttatgct gctaatctcg acataaagaa tatggttaaa
     6541 gatgccgatt acaaaggtgg attttctttg caaggaagta aaatggtagg ctcaggcaac
     6601 agacgacagg atgcatcaat aacttttaat ctgaatggta aacctcagtt gaaattgctt
     6661 tcgcaaggat ttatttttcg tacagacagg attgtatctg taaatgcagc tgctgtaatt
     6721 tattgggaga aagattcaat ttatcatccc ggagttgagt ttaaatatat ttatggtgat
     6781 aaaactgtaa cgctgaccaa aaacggtcaa actgccatca atagcccgta ttttgacagc
     6841 tatcataaaa tggatttaga ttttgatcag ttggtatgga agattacaga cccattgatg
     6901 gacctaaaaa tgatttcagg tggaggagag agtaagctga aatttgagtc tgtaaatttc
     6961 ttttcacgac agcgttttga taaaattcag ggactgtctg aagtacatcc gcttttcaaa
     7021 ataaagcaat atgcagagga gcataatgta aaagttatca gtgtgccgga gtttgcggaa
     7081 tatttaaaac tcactgaaaa cactgtgcgc aatcaaattc tgcagttgtc atcattagga
     7141 tttatttctt atgatgccga tgccgataag tttattgtta aagacaaggt gatgtactac
     7201 ctcaatgcac gcaccggtaa ggttgattat gatgagttga aaattgaatc tatgatttca
     7261 ggattatcta atgcaaccat caacttgttg aattttgata ttaacatgcg tggtgtaagc
     7321 catgtattaa tcagcgattc gcaggaggta tatattcaac ctttggatca ggaaatgact
     7381 ctgaaaaaaa atcgtgacat tgttttcaat ggacgtgttc atgcaggaag acttgatttt
     7441 tatggtaaag agtttacttt taattatgcc gattttaata ttgatttaaa gaatgtggac
     7501 tcactgcggt ttaaagtgcc tgctgactct gttgatgaaa gaggaaaaaa acgtttgatt
     7561 ccaataaaaa gcattttgca gcagttgaat ggaaaacttt ctattgataa cccaacgaat
     7621 aagtcagggt ttgtacctaa tgaggaatat cccgtttttg tatgtacaaa caagtcttat
     7681 atttactatg attatcctga aatattcggt ggtgtttata agcgtgataa gttttattat
     7741 catctcgacc cattcagtat tgacagcctt aacacactta ccaaagaagg acttgtattt
     7801 gaaggaagta tggtgtctgc agatattttt cctgaatggc gacagcaact tacagtgcag
     7861 cgagattatt ctttaggatt tattaccaac acacctgatg ccggttttgc agcttatagc
     7921 ggaaaaggaa aatatcggga tcaaatcagc ttgagtcatg aaggtttcct tggaaaagga
     7981 gaagttgatt acctgagcag cacttcaaaa tcaaacaata ttatattctt tcctgacagt
     8041 atgcatgcag ttacacaaag ttttgcaata agaaaagagc aagttggtgc tacccgtttt
     8101 cctgatgtta agaatgaaga tgtgtttgtt aactggcgac ctaagagtga taaaatgttt
     8161 gtatttaaga gaagcaaaga ttttgcattg tttaatggtg aggcagttct cgatggaaac
     8221 ttagtgctga tgccttccgg aattaaggct aatggtataa ttcgttacca acaatctcag
     8281 ttaatcagca acaatcattt tattaaagaa catagctatg gttccgattc tgcagaattt
     8341 aaactcaata cagatgcagg agatttgctg gcattgcata cacagaatgt aaaagccaat
     8401 attgatcttg ataaacgtat tggggatttc agcagtcagg gtgaaaaagc taaggttgag
     8461 tttcctgtta atgaatatat ttcttacatc agagatttca aatggtatat agataaatca
     8521 gaacttgaat ttggcggctc tgatgcaact gccgacaaag caaatgagtt tgtgtctgta
     8581 aacagacagc aggattcttt acgatgggat gcacctttag caacttataa cttaaaatcg
     8641 tacttgctaa aaggatttaa ggtgaaagaa attttagtag cagatgcttc aataattccg
     8701 gatttaggga atgttgttgt agagcgtaat gcagtaatgc gaccattggt taacagtaag
     8761 gttattgcta atactacttc gcactatcac actatgctaa atacaaatat tactattcag
     8821 agtagaatga attattcagg agatggcaat tatgaatata tggatcagac aaaggttaaa
     8881 cataaaatta aacttacaca gattggagtg gacacttcaa aacagacttt tgccattgga
     8941 gaaattccag attcatcacg tttttttatt agtcctacaa ttcagtataa aggaaagtta
     9001 aggattaatg caccaaaaga aaatctctac ttcaatggat ttgcaatcgt taatcatcac
     9061 tgtaatactt ttgaacgcaa ttggtttggc tttgcaggcg acattgaccc aaaaggggta
     9121 aacattccta ttcaaagtcc ggctaacgaa agtttgcaga aactttattc aggaatttat
     9181 tttggcagcg acagcaccat gatgtatcct gcattcttat caatgaaaaa acatagttca
     9241 gatgcagaag ttatttctgc agaaggctat ctgacttttg ataataattt gcagcagtat
     9301 aaaatttccg gtaccattcc cggtaccgat tcaaccaagt cagcaagtgt tattactaat
     9361 acagtaattc ttgatgacaa gagatgtcag ttgattggtg agggccgaat aaacctgact
     9421 caggattttg gtcagctttc aatcacaaat tttggtatgg tcaactattc aacagttaat
     9481 gactctatag gctttgatgc agttatggca actgacttta tgttcaatga tgatgcattg
     9541 aaacaaatgg cagagatgtt taattctatt cctgcattgc aaccttttat ggacgataga
     9601 ccggcttata gaaatgcatt gactaacatg attggaaaaa ataaagcgga aaagttattg
     9661 aatgaaacag ggctgtacgg ttcggcacga agaattcctt ctgaattgca gcatacgctt
     9721 atgttgagtg agcttaaatt ttattataat aaagaacgtg catcttatat ttctgtagga
     9781 aatatcggtc ttggtgtcat aggaaagacc tatgtaggaa gaatggtaaa aggcaaagtt
     9841 gagattatgc gcagacgtag tggagatgta ttgaatattt atctggaagc attgcctgat
     9901 atgtggtact attttaatta tcagcgtggt gtattacaag cgatttcttc tgatgaaaaa
     9961 ttcaataagc caattgatga aatgaaacca gaaaagcgca tagcatcgga gaaaggaggt
    10021 cttgaaccct atcagtttat gcttgccacc gagaggaaaa agaatgagtt cctgaaacga
    10081 attaaggatg cagatttgta agatgtaaat tttagatggg ttgaatttat taaaaccaat
    10141 aattagaaat tatttaatgc aaagagaaca tcattatcag gcaaatatta tttggacggg
    10201 caacaaaggt tcaggtacag atagttattt aggttacgca agaagttata ctgttaacat
    10261 tgataataaa cccgaaattc aaggttcagc agacgctgca tttcatggag ataaaacaaa
    10321 atataatcct gaggaaatgt tgttgatagc attggcttcg tgtcacatgt tatggtattt
    10381 gcatttatgt tctgaagcag gagtggtagt tgtagaatat acggatagag caacaggtct
    10441 aatgacagaa acaccatcag gcaatggaaa atttgaatct gtaacattaa acccaatcgt
    10501 agtgattaga gatcagacaa aaaatgaaat tgccattgaa ttacataaga aagcaaatga
    10561 gttttgtttc atagcaaatt cagtaaactt taaagttaat cacaagcctg ttttcaaatt
    10621 gatgaaatga gcgtatttta acaggaaaag ctttttttga aaaagcaaaa ttcctcttgc
    10681 ggcaacatga aaatggaaac ggctttatga ttttttcata ctttagctcc gttttggtat
    10741 cattatagtt agcaaacaat ttaaataaaa atcttattca aatgaaaaac acattcttta
    10801 cagtggcatt tattctttgt gctttttttg tgaaagctca atcctcaaat gttattctat
    10861 tcaccgagaa tggtgaaaag ttttttgcta tcctaaatgg agttcggcag aatacaagtc
    10921 ccgaaaccaa tgttaaagta acagggctta atgcaaattt ctacaaacta aaagtgatct
    10981 ttgaagacac taagttggga gagaaggatt ttaatcttgc cttcaatccg ggaatggaaa
    11041 ccactttcag tattaagttt attgcaaaaa agaacagtta tgttttgcgc ccattgagcg
    11101 aagtgccttt agcacaagca ttgcctcctg cagctaacca aaccgttgtt gcttatagtg
    11161 aagtacctgc gccaccaccg gcagctacaa cggttactca tactacaaca actacaacaa
    11221 ccgatggaac acccatgccc ggtgacaatg tgaatgtaaa cattggtgta aatatgaatg
    11281 aagcaggtgg cggaataaat atgaatgttt ccggaggaac aacacaaact actacacata
    11341 ctaccacaac aacagttacg cataccacta catcagaacc aactgcacca cctatagcac
    11401 ctccaccccc tgcaccggtt gtttatttgc cgggatataa tggcccttat ggatgtcctg
    11461 ttccgatggc tcctcaggat ttttctgagc ttaaacaatc catatcttca aaatcttttg
    11521 aagacagcaa gatgactatt gcaaagcaag tgcttcgtaa taactgcctt tttacctctc
    11581 aggtgaaaga tttgatggga ttgttcactt ttgaagagtc caaactggac tttgcaaagt
    11641 atgcttatga ttacacctat gatattggaa attattttaa agttaacgat atgtttacat
    11701 tcgagtcgtc aattgatgat ttgaataatt acatcaatag taaacattag aaaatatttt
    11761 ttttaaagat agccgggaat atttgttacc cggctttttt tattaagaaa acaatttaat
    11821 ggttggggat ttttatccaa tcactatatg tcatttcaaa cttaatttct tttgacctat
    11881 gagttccgac ctctgtccag cctgcttttc ggtaaaattg ttctgctctt gtattaaagg
    11941 cagttcctaa ccagacggtt tgctttgttt gggaaaaata ccagtctaac attgtcttat
    12001 gcaaatgtcg cccgataccc attttctcaa attttggatc taaaaacagc gcccaaatgt
    12061 tattctcttt caagtcagcg attgcaaaac ctactatttg attttcaatt tcgcaaaccc
    12121 aacctttacc tctttcggaa atatattccg cgcaatcttt gtccgtcact aaatcgggat
    12181 tagataaagt gttttctttt acagaatttc tgactctttg aatctgtttt atgtcgtcaa
    12241 ttttagcttc cctgattacc atttatttat tatcaggttg aaagtttttt tcatattaat
    12301 tagtaaatgt tgtttctgtt tgaattgttg tggtttgaga actgacttaa agattactga
    12361 atgtactata caactgttgc aaataacttt ttgcagtaac ggtgtcggca attgcgtaat
    12421 atggtctttt gtaaataaat ttttgattgc tcaaatcata gacaaatgta caactgtctg
    12481 ttacccatcc ttcggcatca tccatgcata aataagcaaa gccatttcgt tttcggttaa
    12541 gtaaatcgtt gctccaatta aacatatcac tattaaaccc taactgatgt aataaagtgg
    12601 aagggatgtc gtgatgcgag cctgtgctgt aaacaatttt atttttataa atatttttta
    12661 caacatcacc tgccattacc attggaatgt gtctgatgta atgactgtag tatgacctgt
    12721 cacgaggtat tttgttgcca tggtcagcaa ctataacaaa taatgtattt ttaaaccaag
    12781 gttctttttt tgcagcggca agataattgc ccaaactgtg atctgcatac catgctgaac
    12841 ccttaaactg ctcgtttggg ttatctttcc caaaaggcga ctgaatagga atgtcgtatg
    12901 gctcgtgaga acttaatgtc atcacaacag aaaagaaagg ctcttttgct tgatttaaat
    12961 cgttcaggtt tttttcaaaa acaaactcat catgtgctcc ccatttggcg ctcaattgct
    13021 gttgtttaaa tttcttttta cctgtaattt gctcaaagcc acagttgact aaataggtgt
    13081 tcagatttga aaagtcaaga tcgcctccat aataaaatga agtgctgtaa ccttgctttt
    13141 tcaactcagc aggcaaggag ggaagcttag cagtcttatc cggaaaacgc atgatggatt
    13201 gattaggctg tgccggaaat ccactcaata ctgccacaaa tgcctgatcg gtgcgtgcac
    13261 cactactgta aatattttta aatgataatc catcgtgaat taatgaatct attgtgggag
    13321 aaacaccggt ttcaccaccc atagaaccta caacatcaac tgtaaaactc tccaacatga
    13381 tgatgacaat gttgggtttt ttattgttca gtacaggcat taaatcaatt ggtgtttgaa
    13441 ataatttctt tacatgttct tcagcaactg catcactata aaatttgtaa tatgaagcat
    13501 tttcactttt ggcctgattg agattgtgcc ccaaatgcca agcaggattt gtagcgatgt
    13561 gattaagtgt tacattctca ctgaaagcag cagcactctc atttacaggc agcatttgca
    13621 cgccaccacg aaacattatt acaagcattg cagcggaaac aataaattga agtaatgatt
    13681 gaataattgc aagtttgtta tcaaaaaaat gttttgtaaa ttttctgaat acaaaccatg
    13741 taagtataaa taaaagtata atacatatta ttcccacaat caattcaaaa ttgttcactg
    13801 acaaaaacat ctgccgccag tctgctaaaa aagataatgc tctatagttg attagtacac
    13861 cccatgaacg ataaaggaaa agattactta catgcaataa aattaaaaca aacagaatta
    13921 ttccattata aacaagaaac aactttttta taaaagtatt ttctgttaat gcatggatta
    13981 acattaaaag taaaggcagt gccgaaatgt atgcagttgc tgaaatgtct agtctgatac
    14041 cataaaacaa tgtggaaggt aattcattta gttgaatgtt tgcggcatta tactgaagaa
    14101 acaagaaaaa gatgattctg aaaatgccga agaacagcat ccagaaaatt gacagcacaa
    14161 gaaaatttct aatcaaacgc atgttaatca gccttaatta attttccatt acctgttcct
    14221 tcatgcacta cgctgtcggg attgccgaag taataaatgt cgcctacata agaaatttta
    14281 gcagtgagcc attgcttagc tctgacatat tctttgttag cacctttgtt ttctgtaaaa
    14341 gtaacctctg tattcaggtt gcttgcatcg gcaatgccat tgccgttatt ataaaaataa
    14401 tgtacaccca cataaccatg aaagacaaaa tcacccggtc ctgtatgaat gttagttctc
    14461 acagctccgg tgttaaaaag aaaagtcacc ttgcccgatg caccccaatt gttgaacgta
    14521 aactcattat gtcgaatggt gtccatcagg tcaatgtttc ctgaaccatt tactgttagt
    14581 tctgcaatat caggcatcca aactttgaca gtaaatttat tcttaaaact tctaacccaa
    14641 ttacatgcat tttcgtttct gatgtacagc atgctgtctt ttacttctgt tgtaatgcct
    14701 tcaatcaatt ttgaacctgc cattacttca acttgataat cataacccgg ataaattttt
    14761 acatctacat tgttgcttgc tttaattcgg ttaaatccgg ttatatttct tatttcagat
    14821 ttgtcatttc ctgtcccttt aaagcaatca aacatgtggt ctttctgaca ggaacagaag
    14881 acaagcaaca gaagaaaaca aaaccagtta atgatttttg ccataggcca gaataatgtc
    14941 ttttaccaaa cgatgtcgga caacatcacc tgcatcaaga gtgataaagt caatgccttt
    15001 aatgtttttc agaattttca ttgcctgaat caaccctgaa ggttgtttgt taggtaagtc
    15061 aatttgtgtt acatcgcctg tgacaataaa ttttgcagca ggccccattc ttgtaagaaa
    15121 catttttagc tgactctctg tagtattttg tgcttcgtca agaattacaa atgcatggtc
    15181 taatgttcgg ccacgcataa atgccaatgg tgctacctca ataactccgt tttctataaa
    15241 ggtctttaac ttctcacccg gaatcatatc aaacaatgca tcgtaaagcg gacgcagata
    15301 aggatcaatt ttttctttta agtcacctgg taaaaaacca agattttctc ctgcttcaac
    15361 agcaggacgt gtaagaacta ttctcttgat ttctttattc tttaatgcgc gtactgccag
    15421 tgctacggca gtataggttt ttccggtgcc tgcaggacct attgcaaaca caatgtcgtt
    15481 tttattgcaa ctgtcaacca ttttttgctg attggcagta cgtgctttta caacaacacc
    15541 atttggtcca aagaccaaaa catcactgcc atttccggat ggtggattta ctaaagtatc
    15601 agagttttgt ccgtcagcca ttaacttttc caatacctgt tcatcaaatt ttccgtagtg
    15661 atgaaaatat tcaatggcac caatgatttt ttttgccacc caatgaattt gcttttcatc
    15721 acccaaaatt ttaagctcat tacctctgac aatcagctta aaagcaggga atttttttct
    15781 taataaatct aaattactat cgttgatgcc aaaaaatgct acaggatttt ctaaatcaac
    15841 gggtatggtt aattcactca attttacaat agtttaatgt atgcaaacct aatttttttt
    15901 ggacacatca gcgattgttt ttcttaaaat tccggtaatc ttcactaaca tcagaaagtt
    15961 tataatttgt caatacagta agatgtgctt acagtagtac ggatattttt gtgaaattaa
    16021 ttccggcagt attcatgtca ttaattacac tcacaacaga ttggggacta accgatcact
    16081 atgtagcagc actaaagggc gaattgctga gcatgttgcc gggtgtgaat attgttgaca
    16141 taagccatca gattccggct tacgatttta ctaaggctgc ctttgttttt aaaaatgcat
    16201 tttacttttt tccgcctcaa acaattcatt taatctgtgt tgacaaacaa tctaagaaaa
    16261 cagaagacac acattcaggg tggatggtta ttcaatatgg agaaagcatt attgtttgcc
    16321 gcaataacgg tttttttacc ttggtgagta agcaagagcc attaaagtcg gtttacattg
    16381 aaggttcaga agaggtgagt gtggcacatg agaaaagttt tttagttacc ctgctacatg
    16441 atttggttca ccataaacct ttggaagaaa taggacagcc gataaaatct ttttatcaat
    16501 catcaatgtt tcagccatcg cacgatgcca acaccattca gggaacggtt attcatattg
    16561 atacttacgg caatgctatt accaatattg aaaaggaata ttttgagaat gtagtaggag
    16621 caaaacgatt tgaaattgat atgcctcgcc acggtgatgc aattactaaa atatataatc
    16681 gctatgatga tgttactccc ggacagttgg tagcgctgtt taatgcatcg ggctatcttg
    16741 aaattgcctt aaatcagggt gatatttcat tgttgtacgg acttgagtat ggagataaaa
    16801 tcagaatttt tctgcaatga taacacgtat tgtacgattg cccgttaaac ctgatgaggg
    16861 caaaaatttt gaacaactat ttgatacaat aaaagaaaaa attgcagcct ttgatggatg
    16921 tttgtcagtt gaattgcata aagatgtagc ttcgtctgat tattttacct acagcctttg
    16981 gcagagtgaa agccatttgc aagcctatct cgactcatct ttttttacag acgtttggcc
    17041 taatgctaag aaactgttgc atcaacgtgc caatgcatgg tcgcttgaga aagttttgaa
    17101 tgtttggaaa gttgctaagt tttgaaagtt agcaagtttg aaagtttaga atgtttgaaa
    17161 gttttgaatg tttgaaagtt ttgaatgttg agatttaaat tacactgaca agggcggtgc
    17221 gacatgacag gtgaaattaa ctgttgcttg aatgatagat gggcacaagt ccgaagactt
    17281 gtgccagagg ggtttagaat gttgaaattt aaattacact gacaagggtg gtacgatctg
    17341 agaggtgaaa ctgaatttgt tctattttca taaaaaaagt attaaccctt caatgtgttt
    17401 atattatttc aaagtcctct gaaatgcgag agactttgaa agtacggaaa cccacatgca
    17461 ctataccatg tgttacctgc tgggcttttt ccattgtttg ttaaatacat attcaaccat
    17521 ttcttcaata ttaactattt gtcttgttga tgcattcatc cgaatatata gttctttctc
    17581 aatgaattca ttttttttgt ttttaagaaa tattggtttg taactttcgg taataactat
    17641 tactaaaata tctttaccct ctactttctt aattgataaa tctactaatg gctttatgga
    17701 taagtcaaaa aaataggcca aaagtgagtc aaattctaat aatagcttat ccttttcatt
    17761 ttctccatga aacaatgaat aatcataatt ttctaatcct tgaatattac cgttatcatt
    17821 tactccaata aataaaactc cgccatttga atttagaaat ccgcaaatag ccttggctat
    17881 taaatattta acacctattc ctgcggcttg agttttaaaa ttataaagta aacatggttt
    17941 aaattcgact tgattacttt cgcctctttt aataatactt agtaaatcta tgccctcatt
    18001 aatatcagtt tcttctgtat cttgatttga atcataaatc tcattatacc tttcaatagt
    18061 atcccaaaca taaggtgttt ttagaattgt tctttttgct ccgccttctt catcttctcc
    18121 tgattctgca tagtctaatc cataatgata aactgtcgtt tttaattcac caacatttat
    18181 ttgataatat t
//
LOCUS       DAORQT010000011        17879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_259_length_17879_cov_14.873710, whole genome shotgun sequence.
ACCESSION   DAORQT010000011 DAORQT010000000
VERSION     DAORQT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17879
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_17879_cov_14.873710"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1589
                     /locus_tag="PK134_00925"
     CDS             219..1589
                     /locus_tag="PK134_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB37447.1"
                     /translation="MLKKITAFATAFIVAGTAMAQKNLPSNFHMKKLGNGLEVLVIED
                     NSVPLVTIEICVKNGSFTEDSAFNGLSHLYEHMFFKANKAIPSQEKFLERVNELGISF
                     NGTTSEERVNYFFTLSNKLINEGLEFMNNAIRYPLFLEQEMKNENPVVDGEFQRAESN
                     PAFFLFQDFNKQMWGSNYVRKNAIGIHDVILSATPAKMNVIKDKYYYPNNSILVVAGD
                     VEHNAVFEKAEKMFGDWKPCDFDPFQKWPIPEFAPLVGQSKFLTVDKNAKMPIMMMGF
                     HGPDTRNDVKSTYAADVFSTILGLTSSEFQKTLVDGGYALQAGLGYQTCRYTGPIQLF
                     MVPNPMKMKSFYGKLMEQVNRFDAPDYFTDEQLETAKNKLIMDDIYNKEKTSAYVHTV
                     TFWWASASIDYYTTYNDMIKKVTRKDIADYVDKYIKGKPSVTGLLLNPKMKESLGINS
                     ADEILK"
     gene            1601..2932
                     /locus_tag="PK134_00930"
     CDS             1601..2932
                     /locus_tag="PK134_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB37448.1"
                     /translation="MNKLYKYFAIGAAMLSIHGVALAQATKEVNVDGLKVILRHTPKE
                     VISVRLYVKGGTANYTKDKEGVESLAFSIAMNGGTKKLDKVAFNDAAEKIGTSFEASS
                     GLDYGNLSMTCIKPNWNESWNLFADAVMNPAFDSKEFDLEKEKALSGAKQRESDADGS
                     LSNIAMEETFKGRNYAKDPQGSVASLEKITLADVISHYKNVIGKKRCYLVVVGDVNEA
                     DLVGKIKATLGKMAVGTAPVVEPKLVITKGSEVIVDRDIATNYLMGIMSAPSLSTEKG
                     VQMDLAMQMLYDRFFVELRTKRSLSYAPAAGYMRNAVSSPYSYIYITTTDPKKAIDVM
                     VSIIDSIKKTGFTEKELKDLRQGYLTNYYQKLETSEAQSGALGRSEATGSWKLDESLS
                     DMVNNVKLKDLNNTFNEYTAAIKWSYLGKKDAISKDDFKQTKHDKVLQSPY"
     gene            complement(3022..4200)
                     /locus_tag="PK134_00935"
     CDS             complement(3022..4200)
                     /locus_tag="PK134_00935"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRB37449.1"
                     /translation="MKEVYIVSAVRTPMGSFGGKLSSFSATRLGAIAIKGAIDRIKLN
                     PSEIKEVYMGSVLQANLGQAPARQAAKFAGLPDTVQCTTINKVCASGMKAVMLAAQSI
                     MLGDNDVIVAGGMESMSNVPFYMQNMRWGNKYGDVSAVDGLAKDGLTDVYHNYAMGNA
                     ADLCARECNFSRQDQDTYAIQSYKRSADAWAAGKFKDEIVPVEIPQRKGDPILFAEDE
                     EFKNVHFDKIPGLKPAFSKEGTVTAANASTMNDGAAALVLMSKEKAESLGLKPLGRIR
                     SYADAEQAPEWFTTTPSKALPRAVDKAGLKMTDLEFVELNEAFSVVALANMKEMNLDP
                     AKVNVNGGAVALGHPLGCSGARILVTLLNVLKQNNAKIGGAAICNGGGGASAMVIERM
                     "
     gene            complement(4351..5343)
                     /locus_tag="PK134_00940"
     CDS             complement(4351..5343)
                     /locus_tag="PK134_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRB37450.1"
                     /translation="MKIKVSLLIATAALSISTVIAQEGFRTIAGKKCQLYKVVSKDTW
                     SGLSRKYHKEIDELKAVNPGINDLKIGQIINIPIENTVNDKSENKSDTSAEIKMRKPI
                     AVINAPVKNNNKVHVIEKGETLFGIARKYDVSPNDIRKWNHLDNDGIKAGQKLIVSST
                     ESVAIKTEVKKQESKNETILQPLDDKKLEAKNNEVKNDSKLIAEASNKVIPPSPSPSE
                     MEYKPMRTEVKRSNGKSGAVIEVNESGMASWIRDGDINQSKFYALHRNAPPGTIIKVT
                     NRMNGDYVFVKVVGTLPDTGDNDKQILKMSEAAAKRIGAINEKFQVELNYGMTN"
     gene            5489..6811
                     /locus_tag="PK134_00945"
     CDS             5489..6811
                     /locus_tag="PK134_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRB37451.1"
                     /translation="MKKLFLALVLLFFATTTVNAGIVYRFNFQSEVDRGMARIFSKAL
                     QEAHEQKADLFLIHLNTYGGMLDAADSIRIAILNSKIPVVVFIDPNAASAGALISIAC
                     NRIYMRSGSSIGAATVVTEQGEAAPDKYQSYMRGIMRATAEKRNRDPRIAEAMVDPRV
                     VIPGVNDSGRVLTFTAEEALTNKYCNAIVETEADILKLENLNSDKIIEFQPSWVDNII
                     SFLIHPALSSLLILIMLAGLYFEFQAPGTIFPIAASFVAAVLYFAPLYLEGLAANWEI
                     LLFVSGVILLALEFFVIPGFGVAGIVGIICTTMGFALSMLNNKGWDFTFTSSEQITQS
                     ILISLGAMLGAVFLIFYFIGKINDSKRLSKLTLQTTQPSEHGFRAHTDIDTIQAGMRG
                     TAFTALRPSGKIMVDGRVYDAVAETGWIERQEQVEIVSVGLTCTVRTC"
     gene            6937..7803
                     /locus_tag="PK134_00950"
     CDS             6937..7803
                     /locus_tag="PK134_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37452.1"
                     /translation="MKNILKFSVVFLFLAVYACKDYKPEVDRLNRERDSLINTSFLKD
                     STIDSFLGDFNGIEASLDSINQIHAAITLDRKRDPEMGGTVKDRIRKNMESIASLLDQ
                     NSQRIAELTRKLKSSGVKSAQLNKMITSLNLKIAERDSMIANLNNQLGDAKIEITGLR
                     TNIETLNSTIGEREKTIEEKVNQINTAYYAIGTYKQLRDKKILNKKGGFIGIGKTKTI
                     NPDIDNSAFTKVDIRQFASVELNTKSVKIMSSHPASAYTYEKDKKKYQSLKITNPDLF
                     WSNSKYLVIVTK"
     gene            7872..8273
                     /locus_tag="PK134_00955"
     CDS             7872..8273
                     /locus_tag="PK134_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRB37453.1"
                     /translation="MKPFVVRVYGILINKNSEVLLTHEKEGGIEFTKFPGGGLEFGEG
                     LRECLVREFLEETKQRVEVMEHFYTTDFFIESAFNPGLQLISVYYKVSCINFILPEDA
                     NDIHFFWKPLKELNGEVATFPVDRLVEKLLM"
     gene            complement(8274..11042)
                     /locus_tag="PK134_00960"
     CDS             complement(8274..11042)
                     /locus_tag="PK134_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRB37454.1"
                     /translation="MKALVMNNKTDSFLTRIVDELIATVDGNNFADYTVVFPTRRAAL
                     LFKLQLAKKLNKAVLLPSVLSISDFVEHYMPLRVAEKKEVLLDLFAVYKPFFPDYDFT
                     SFAPWGEMILGDFEEINLYLVNYKDLFADLTSYHEIESVFQDDDEFKEQLKQFFELFS
                     TDKTSTIKQNFLYAWQALPKLYEKLNEVLYKNNKTTAGKAMQQLALSPETYLKNLDCS
                     KTIIAGFYALAPAEQKLFEYILNGKGKIFWDADEYYTKNTTQEAGLFFRKNFLTQNSF
                     KWQETYFKDIPKNINVTGVPLITGQFKFLADRLKTLIAQNKLNTDKTAIVLPDEKLLP
                     LLLQSLPEEIEHVNITMGFPLKETHLYRFIETLNVLLNQISETSKQKFVSTFALGNYL
                     AHPYASLLKHDIRNLNDAGISIPLNELPENISQFLKSQITSPIFEGYIQLLTMLHDAM
                     EPKHHEKEIAGFVLNELLELRTMVIPYTEILNEQALQMLISEILDTTRIPFSGEPVKG
                     LQVMGLLETRTLDFENIFFLNVNEGSVPKSNRHHSFIPFTIRKSFGLPLLDEQDAITA
                     YHFWRLMQRAYEIDLIYNTEVNEFASGERSRFLLQLFYEMKLHFSNWQVSHQIITTPL
                     YRYNYITPGVKRDEQLTNHLLSMVAENKLRFSASSLNSFIHCSLQYYFKEIKKIREPD
                     DNGEEIDAAVFGKILHDAVSKIYAQIIDVEFDKDNAAQLKKSISAFVKASIAQEYDKH
                     YQGTGYTVIIEKIISEYIERIIAADLKSETFSPHELETEKVVVCDIGNGINVKIKGIF
                     DRIDKVSDGYRIVDYKTGSDVVKNIKKPDELTSEPKYKVNFQLLLYCWLFLKSHSNEK
                     VNAGIYPLRRMEAGIEMLGQQPLTDHSKFEELLFALIRKILLESDFTMTTDTLRCKYC
                     PYKDICNR"
     gene            complement(11017..12270)
                     /locus_tag="PK134_00965"
     CDS             complement(11017..12270)
                     /locus_tag="PK134_00965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB37455.1"
                     /translation="MDHSTKTFALSNGLQIVLTTLSNTEVSHCALLIKAGTRNEQIQQ
                     EGLAHFIEHMLFKGTRKRKSFHILNRLEVVGGELNAFTSKEETCIHASVTTPHLERAL
                     ELISDITFNSVFNLKEIEKEKEVVCDEIRSYLDNPGEQIFDDFESLVFKKNPLGNPIL
                     GTEQSVKSFKQKDLIAFTRKFYIPSNMVLSIATSLNAEKVIRQLEHHFGKYRLAKVGE
                     RPKPFKKYTAEKSSVKKHSAQCHFIMGTLAPSLYQADRMTVALLNNILGGPGMNSKLN
                     LGVREKYGYTYAIESGFNSYTDTGMFHVYLATDKKFLNKSIDLAVVEMKKLTDNKIGS
                     LQLSQYKQQLKGQLALAKENKSNVVISNARSLLNFKKVFTLEDTYKKIEAVTSSQLTE
                     AAAKYLNAKSRSYSSLLYESVGHEQ"
     gene            12276..14039
                     /locus_tag="PK134_00970"
     CDS             12276..14039
                     /locus_tag="PK134_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37456.1"
                     /translation="MQKYALKVRQRLADYISLPDKTKVTVMQQQKCLNFFILFILSCL
                     FASDIEAQQYKFAKRVGGTQFENAMGIALDSTGNIIVSGYFSGTADFDPSTSNYSLTS
                     NGGNDIFLARYDSAGNFIWAINVGGAGEEFNYSDPAVDEYGNIYLCGVFNTNTNFNPK
                     GIAVMATNRGQQDGFIAKYDSTGLLQWVRGVGGVLNDDVYRIDYKDYLVLFAGSFTDS
                     SFVDDGLTVTPIYGSGNADVVFGKFNPNGNLFWLNTLKGSGEDHSYNITAGINGKTYL
                     TGTFEDTLIFDYVSVSPDTLFADGLAAFTASFSNMGNYYWSFPIENAYPFGLKVDHNG
                     DILTCGQFSGIADFDPDADTMALMAQGSFDGYFAKYTNGGSYDFAKRIGGNGADICYT
                     IAELSNSSILVSGYFFNTADFDPDVTVASLNSNGFADMYLAHYDSMGNYLSAFGCGSP
                     AFEFCRNMTCNGIDEVFLCGGFEQVVDFNPAPAINTLVSAGSRDGYFAKYAFPTTSLN
                     SISADNLMIYPNPFISQINISGDINQTQLILTDVLGNTIYKTKTNSQTTINTEKLNPG
                     IYFLTVVSGENVSTRKLLKVK"
     gene            complement(14331..15656)
                     /locus_tag="PK134_00975"
     CDS             complement(14331..15656)
                     /locus_tag="PK134_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37457.1"
                     /translation="MRLFLTLISLFVVLDTQAQFIGHNGVYFLGTANMPISTTSFNDP
                     NISGVVVRFRWNDLEPSPGNFNWAFIDGEIAKAVTYNKKISLQPLGIPNWLGSIGAQQ
                     YYTIQDNPSSPNFGQITTGVIPWDSVYVSRFKIFLQNLAIKYAGNPVVTYVNVIGVNF
                     SRNLPDTVLTDTVTITKQAFWSVYNYNADSLGALINEMTDYYMSVFPSTPLWCSVDYV
                     TFQPNASGQARNYLASIYCNYGIINYPDRFGLFREDVSGCNPNLSNIANTSHWYLMQQ
                     NHCRTGAQMLWSVQDGPSRMNQCGILPNTKAMVLDSAVNNGLALGMRYLEIYGSDITD
                     TSLASTIQQANNDLIAKGLLCNSSTGLNEPISENTFSIYPNPAREYLTINFDPNQKTQ
                     IQIFNSMGMLFKEFSVTTSTQLDISDLPNGLFFVRLKNYSQPIKKFMKQ"
     gene            complement(15653..16699)
                     /locus_tag="PK134_00980"
     CDS             complement(15653..16699)
                     /locus_tag="PK134_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37458.1"
                     /translation="MKRLLLILGLFISSSGMAQLTGLTLTGNPESLNGATWTYQQTIN
                     GTSYDLTGILFKPVGSGVFPAVIINHGTGGNVNGYSKNVAKKMVQWNYTCIAVNLCHS
                     AGAPIGSPGDSSLINFGASTNNYLRDMKCWDILASLNYVDTNCIMAFGHSRGAYLTTG
                     LVATYPDKFRAAGHTAGGVSPQLGFSAPTTMLAAQISCPYIIHHGDADNTVPIEMDSL
                     LNSVFNSTGIPHQFYQYSGYTHSSISMDSLMFARTQQWFALHGCANTTSINQQISSEM
                     ISIFPNPTSDFIQFKTEKRIDVSLFDVSGKLLLSKIVMPNEQINVSQLNNGIYFITIN
                     LYRKMFHHKIIIQK"
     gene            complement(17076..17516)
                     /locus_tag="PK134_00985"
     CDS             complement(17076..17516)
                     /locus_tag="PK134_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HRB37459.1"
                     /translation="MRVVFFVVMSVFVLSVFAQAKAEEYSLTIEVESLRNSKGILQVA
                     LYNKDGSIPDEYYKNYFKIETAQITNGKSKIIFKNLPKGKYAVNILHDENNNGKIDKG
                     LIFPKEGIGFSNYSSIGLTNRPNFKKASFDLFQDKEIKVKVIYM"
     gene            complement(17563..>17879)
                     /locus_tag="PK134_00990"
     CDS             complement(17563..>17879)
                     /locus_tag="PK134_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB37460.1"
                     /translation="GLVTKNSILIVDFTNQLKAEGKPYRQAIIEASKGRMRPILMTTL
                     SMAIGMLPIALATGTSAEWKNGLAWVIIGGLLSSLILTVFLVPMVYYGVDRVKEKLKI
                     KN"
BASE COUNT         5513 a         3117 c         3297 g         5952 t
ORIGIN      
        1 tccttatcct tcgtataatt cgcagctatt cattagatgt aaaaaactat tcatcaggag
       61 tttatacagc aactgtttat tttgattcgg acattaagac tcagaaattc attgttacca
      121 ataccacaaa caattaaatt taattaataa cataactttt ttttaaccat cacgggctat
      181 agaaatagcg ttcttagcct actattaaat gtataactat gcttaaaaaa atcacagcct
      241 ttgctactgc ctttattgta gccggtacag caatggcaca aaaaaatctg ccttccaact
      301 ttcatatgaa aaagttgggc aatggccttg aggtcttggt cattgaagac aactctgttc
      361 cattagtcac tattgaaatt tgtgtaaaga atggatcttt tactgaagat tcggccttca
      421 atggattgtc acacctttac gaacacatgt tctttaaagc aaacaaggca attccgtcac
      481 aagaaaaatt tcttgaaaga gtaaatgaac tcggtatatc atttaacggt actacctctg
      541 aagagcgtgt gaactacttt ttcacactaa gtaacaaact catcaatgaa ggtcttgagt
      601 ttatgaataa tgctattcgt tatccattat ttctggagca ggaaatgaag aacgaaaatc
      661 ctgttgttga tggtgaattt cagcgtgcag aatcaaatcc tgcctttttt ctgtttcagg
      721 attttaacaa acaaatgtgg ggttcaaatt atgtgcgtaa aaatgccatc ggtattcatg
      781 acgtaattct ttctgctaca ccagcaaaga tgaatgtaat aaaagataaa tattactatc
      841 ccaataactc tattttagtt gttgccggtg atgtggagca caatgccgtt tttgaaaaag
      901 cagagaaaat gtttggtgac tggaaacctt gtgattttga tccgtttcaa aagtggccta
      961 tacctgagtt tgcaccatta gtaggccagt caaagttttt gaccgttgat aaaaatgcaa
     1021 aaatgccaat tatgatgatg ggatttcatg ggcctgatac aagaaatgat gttaagtcaa
     1081 cttatgcagc cgatgtgttt tctactattc ttggactgac atcttctgaa tttcaaaaaa
     1141 ctttggtaga tggtggttat gctcttcagg caggtttagg ctatcagact tgtcgctaca
     1201 caggaccaat tcagttattt atggttccta atcctatgaa gatgaaatct ttttatggta
     1261 agttgatgga gcaggtaaat cgttttgatg cacctgatta ttttactgac gaacaattag
     1321 agactgctaa gaataaactc atcatggatg atatttataa caaagaaaaa acatcagcct
     1381 atgtgcatac tgttacattt tggtgggcat ctgcaagcat tgactactat actacctaca
     1441 atgacatgat aaagaaagtt accagaaaag atattgcaga ctatgtagat aaatatatca
     1501 aaggaaagcc ttctgtaaca ggtctgctct tgaatcctaa aatgaaggag tctttaggaa
     1561 ttaattctgc tgatgaaatt ttaaaataaa aaatcgaact atgaataagt tatataaata
     1621 ttttgcaata ggtgcagcaa tgttatcaat acatggtgtt gcgcttgcac aagcaacaaa
     1681 agaagttaat gttgatggtc tgaaagtaat attacgccat acgccaaaag aagttattag
     1741 tgtaagactt tatgtaaaag gaggaactgc aaattatacg aaggataagg aaggtgtaga
     1801 gagccttgca ttttctattg ctatgaatgg cggtaccaag aaattggata aagttgcctt
     1861 taatgatgca gctgaaaaaa ttggtacatc attcgaagca tcatcaggac tggattatgg
     1921 aaacctgagt atgacatgca ttaaacctaa ttggaatgaa tcgtggaatc tttttgctga
     1981 tgcagtaatg aatccagctt ttgactctaa agagtttgat ttagaaaaag aaaaagcatt
     2041 gtcaggagct aagcaacgcg agtctgatgc cgatggttct ttgagcaata tcgctatgga
     2101 agaaaccttt aaaggcagaa actatgctaa agatccacaa ggctctgttg caagccttga
     2161 gaaaataacc ttggctgatg ttataagtca ttacaaaaat gtaattggaa aaaagcgttg
     2221 ctacttagtt gttgtaggtg atgttaacga agcagattta gtgggtaaaa taaaggcgac
     2281 attaggaaaa atggcagtag gcacagcacc ggttgtggaa cctaaattgg taataacaaa
     2341 aggaagtgaa gttattgttg accgtgatat tgccacaaac tatctgatgg gaattatgag
     2401 tgctccttct ttgtcaacag agaaaggtgt tcagatggat ttagcaatgc aaatgcttta
     2461 cgacagattt tttgttgagc ttagaacaaa acgcagcttg tcttatgcac cggctgccgg
     2521 atatatgcgc aatgcagttt catcaccata ttcatatatc tacatcacaa ctacagatcc
     2581 taaaaaagct attgatgtaa tggtttcaat tattgactct ataaagaaaa caggttttac
     2641 agaaaaagag ttgaaggatt tacgtcaggg ttatctgact aactattatc agaaattaga
     2701 aacatctgag gcacagtcag gtgccttggg tcgtagtgaa gcaacaggct catggaaatt
     2761 agatgagtcc ttatctgata tggttaacaa tgttaagctg aaagatttga ataatacatt
     2821 caatgaatat acagcagcaa taaaatggtc gtatcttggt aagaaagatg caatttctaa
     2881 agatgatttt aagcagacaa aacacgataa agtgttgcaa agcccgtatt gatattttga
     2941 aataggttag tgacttctgc taacaaaaag aggctatctc taattgagat agcctctttt
     3001 attgctaata ttaaagtagt attacattct ttcaataacc atagcactgg caccgccacc
     3061 gccattgcaa attgctgcac cgccaatttt tgcattgttt tgttttaaaa catttaataa
     3121 cgtaaccaat attcttgcac ctgaacatcc taatggatga cctaatgcaa ctgcaccacc
     3181 attgacattt acttttgcag gatcaagatt catttctttc atgttggcta aggcaactac
     3241 tgaaaaggct tcgttaagtt ctacaaactc aaggtcagtc atttttaatc cggctttatc
     3301 tactgctctt ggtaatgctt ttgatggagt agtggtaaac cattcaggcg cctgctctgc
     3361 atcggcataa ctgcggatac gcccaagggg tttaagtccc aatgactctg ctttttcttt
     3421 actcattaaa accaatgcag ctgcaccatc attcatggtt gaagcattgg cagcagttac
     3481 tgttccttct ttgctgaaag ccggttttaa tcccggaatc ttatcgaaat gaacgttttt
     3541 aaattcttcg tcctctgcaa aaagaatagg atcgcctttg cgctgtggaa tttcaacagg
     3601 aacaatttca tctttaaatt ttcctgcagc ccaggcatcg gcactgcgtt tgtatgattg
     3661 tattgcatag gtgtcctgat cctgacgtga aaaattacat tctcttgcac ataaatctgc
     3721 tgcattcccc attgcataat tatgataaac atctgtcagt ccatctttag caagaccatc
     3781 aacagcagaa acatctccat atttatttcc ccatcgcata ttttgcatat agaagggaac
     3841 attgctcata ctttccatgc ctccggcaac aatcacatca ttgtcgccaa gcataatact
     3901 ttgtgctgct aacataacag ctttcattcc cgaagcgcaa actttattga ttgtagtaca
     3961 ttgaacagtg tcgggcaagc cggcaaactt tgcagcctga cgtgcaggag cctgtcctaa
     4021 attggcttgt aaaacactac ccatatacac ctctttgatt tctgatgggt ttagtttgat
     4081 tcgatcaata gcgcctttaa tggctatagc acccaaacgg gtagcagaaa atgatgacag
     4141 tttaccacca aaacttccca ttggtgtacg cactgccgat acaatatata cttctttcat
     4201 tatgcagaat tttaattata aaccgcaaaa ataagaaaag taacttgtaa aatccgacag
     4261 ggtttactgt tggttccggt atagaggaga accaatatac tttttgcctg gcagtgcgta
     4321 cgtcaatgta ttataaaaca acattttcta ttaattggtc atgccgtaat taagctcgac
     4381 ctgaaatttt tcatttattg cacctattct ttttgcagca gcttccgaca ttttaagaat
     4441 ttgcttgtca ttgtcgccag tgtcaggtaa tgtacctacc acttttacaa aaacataatc
     4501 gccattcatt ctgttggtga ctttaattat tgtgccagga ggcgcattgc gatgtaaagc
     4561 ataaaattta ctttgattga tatctccatc tctgatccag gatgccatgc ccgattcgtt
     4621 tacctcaatt acagcgcctg attttccatt gcttcgtttt acttcggttc gcatgggttt
     4681 gtattccatc tcactcggtg atggcgatgg tggaattact ttattacttg cttctgcaat
     4741 taactttgag tcgtttttaa cttcattatt ttttgcttct aactttttgt catcaagagg
     4801 ctgaaggata gtctcatttt tagattcctg ttttttaact tcagttttta ttgctacact
     4861 ttctgtactt gatacaatca atttctgacc ggcttttatg ccatcgttat caagatgatt
     4921 ccatttgcga atatcattag gactgacatc atattttcgt gctataccaa aaagtgtttc
     4981 tcctttttca atgacatgaa ccttgttatt gttttttacc ggagcattta ttacagcaat
     5041 aggcttacgc atttttattt cagcagaagt atccgatttg ttttcgcttt tgtcgttaac
     5101 tgtattttca atcggaatat taatgatctg tccgattttt aaatcgttta tacccgggtt
     5161 aactgctttt aactcatcaa tttctttgtg atattttctg gataaacccg accaggtatc
     5221 tttagaaaca actttataaa gctgacattt tttgcctgca atagttctga aaccttcctg
     5281 tgcaattaca gtcgagatgc ttagtgctgc tgttgcaatt aataacgaaa ctttgatttt
     5341 catcccaatc acttttattt ttacaatact aaaaaaatca cttcctcgta aatgaagatg
     5401 ctttaatagg ttttaaacaa attaattaac atcaaaacga cagttgtaag tagtaattgt
     5461 gcaattttga atattcaact taaatgaaat gaaaaaatta tttttagctt tagtgttatt
     5521 gttttttgca actacaactg ttaatgccgg cattgtttac aggtttaact ttcagtcgga
     5581 agtggataga ggaatggcac gcattttcag taaagcattg caggaagcgc atgagcagaa
     5641 ggctgatttg tttttgattc acctcaatac ttatggaggc atgcttgatg ctgccgacag
     5701 tatcagaatt gcaatattaa attcaaaaat tccggttgta gtatttattg accctaatgc
     5761 cgcaagtgcc ggtgcactca tctctattgc atgcaacaga atctatatgc gcagtggttc
     5821 aagcattggt gctgccactg tagttactga gcaaggcgaa gcagcaccgg ataaatatca
     5881 atcctatatg cgtggcatca tgcgcgcaac ggcagaaaaa cgaaatcgtg acccgcgtat
     5941 tgctgaagct atggttgatc caagagttgt tattccggga gtaaatgata gtggaagggt
     6001 gctgactttt acagcagaag aagccttgac aaataaatat tgtaatgcca ttgttgaaac
     6061 agaagctgat attttaaagt tagaaaatct gaatagcgat aagattattg aatttcaacc
     6121 ttcatgggtt gacaacatca ttagtttttt aattcatcct gctttgagca gtttattaat
     6181 tcttattatg cttgccggtt tgtattttga atttcaggct ccgggaacca tttttccgat
     6241 tgcagcttct tttgttgcag cagtcttata ttttgcacca ttatatcttg aagggcttgc
     6301 tgccaattgg gagatattac ttttcgttag tggtgtgatt ttattggctc ttgagttttt
     6361 tgtcattccg ggttttggtg ttgccggcat tgtaggaata atttgtacta caatgggctt
     6421 tgctttaagt atgctcaaca acaagggatg ggattttact ttcacaagtt ccgaacaaat
     6481 aacacaaagt attttaataa gccttggagc catgcttggt gcagtgtttc tgatatttta
     6541 ttttattgga aaaattaatg actcaaagcg cctcagcaag ttgacacttc aaacaacaca
     6601 accatctgaa catggtttca gagcacatac agatattgac accattcaag ccggtatgcg
     6661 cggaacggca tttactgcac ttagaccgtc agggaaaatt atggttgatg gcagagtgta
     6721 tgatgctgtt gcagaaaccg gttggattga aaggcaggaa caggtagaaa ttgtatctgt
     6781 tggcctaacc tgtactgtca gaacttgtta agtgtgaata tctttgttta aatccctttt
     6841 atttgatggt gttttaatga ctggttgcat ctatatttgc agctattaca atccttcttg
     6901 aataactaac tattagaacc actaaaaatt cattaaatga aaaacatcct taaattttct
     6961 gtcgtatttc tgtttttggc agtgtatgcc tgcaaagact acaaacccga agtagataga
     7021 ctaaatcgtg aacgcgactc tttgattaac acatcttttc taaaagacag cactattgat
     7081 tcattccttg gcgacttcaa cggtattgaa gcaagtttag acagcatcaa tcagattcat
     7141 gctgccatta cacttgaccg caagcgtgat cctgaaatgg gtggaaccgt taaagaccgc
     7201 atccgtaaga atatggagag cattgcttca ctactcgatc aaaacagtca gcgcattgcc
     7261 gaacttacaa gaaaacttaa aagcagtggg gtaaaaagtg cccaacttaa taaaatgatt
     7321 acctcactga atctgaaaat tgctgaacgt gattctatga ttgctaattt aaacaatcaa
     7381 cttggtgacg ctaaaattga aattaccggc ctgcgtacca acatcgaaac gttaaactca
     7441 actattggtg agcgcgaaaa gactattgaa gaaaaagtta atcagattaa cacagcatat
     7501 tatgcaattg gaacatacaa acaactacgc gacaaaaaaa ttctgaataa aaaaggtgga
     7561 tttattggta tcggtaaaac aaaaaccata aaccctgata ttgataattc agcattcaca
     7621 aaagttgaca tcaggcagtt tgcttctgta gaattaaata cgaagagtgt gaaaattatg
     7681 tcgtctcatc ctgcatcggc ttatacctat gaaaaagaca agaaaaaata tcaatctttg
     7741 aaaattacta atcccgattt gttctggagt aattcaaaat atttggtaat tgtcacaaaa
     7801 taattttaga gaataataat ttataaggtt gatctaaggg tcaacctttt tttttgacta
     7861 attttgaaac gatgaaacca tttgtagttc gtgtttatgg tattctgata aataagaatt
     7921 cggaagtgtt gcttacacat gagaaagaag gcggtattga atttactaaa tttcccggtg
     7981 gcggacttga atttggtgaa ggtttgcgcg aatgtttagt gcgtgagttt ttagaagaaa
     8041 caaagcaaag ggtagaagtg atggagcatt tttataccac tgattttttt attgaatcgg
     8101 ctttcaatcc cggcttacaa ctcatcagcg tttattacaa agtatcatgc ataaatttca
     8161 ttttacctga agatgcaaat gatattcatt ttttctggaa acctttaaaa gaacttaatg
     8221 gcgaagtagc aacatttcct gttgaccgct tagttgagaa attgctgatg tgattatctg
     8281 ttacagatat ctttgtaagg acaatactta catcgtaagg tgtcagttgt cattgtaaaa
     8341 tccgactcca ataaaatttt tctgattaaa gcaaacaaca attcttcaaa ctttgaatga
     8401 tctgttaagg gttgctgccc cagcatttct ataccggctt ccattcgcct gagtggataa
     8461 attccggcat ttactttttc attactgtgc gattttaaaa acaaccagca ataaagcaaa
     8521 agctgaaaat tgaccttgta ttttggctca cttgtaagct catcaggttt ttttatattc
     8581 tttacaacat cacttcccgt cttataatct acaattctgt atccatcaga aactttgtca
     8641 atgcggtcaa aaattccttt aatctttaca ttaataccat tgccaatatc acaaacaacc
     8701 actttttcgg tttctaattc atgaggcgaa aaagtttcac ttttcagatc ggctgctatg
     8761 atacgctcaa tgtattccga aattattttt tcaatgatta cagtatatcc tgttccctga
     8821 tagtgtttgt catattcttg ggctatggat gcttttacaa atgctgaaat agatttcttg
     8881 agttgggctg cattgtcttt gtcaaattca acatcaataa tctgagcata aatcttgcta
     8941 actgcatcgt gtaaaatttt tccgaatacg gctgcatcaa tctcttcccc attatcatca
     9001 ggctctctga tttttttaat ttctttaaag taatattgta atgaacaatg aataaaactg
     9061 tttagcgatg aagcagaaaa tctcaattta ttctctgcca ccatagatag taaatgattt
     9121 gttaactgct catctctttt gactccagga gtaatgtaat tataacgata aagcggtgtg
     9181 gtaataattt gatgactgac ctgccaattg ctgaaatgta atttcatttc ataaaacaac
     9241 tgtaaaagaa atcggcttct ttctccactg gcaaactcat tgacttctgt gttgtaaatt
     9301 aaatcaattt catatgctct ttgcatcaat cgccagaagt gataggcggt aatagcatcc
     9361 tgctcatcga gcaatggtag tccaaaactc tttctgatgg taaatggaat aaatgaatga
     9421 tgacgatttg attttggaac agagccttca ttaacattaa ggaaaaatat attttcaaaa
     9481 tccagcgttc tggtttccaa caatcccatg acctgaagtc cttttacagg ctcgccacta
     9541 aaaggaattc ttgtggtgtc aagtatttct gaaattagca tttgcaaagc ttgctcattc
     9601 agaatttctg tatatggtat taccatagta cgaagctcaa gcaattcatt caatacaaat
     9661 ccggcaattt ctttttcatg atgcttaggt tccatggcat catgaagcat ggttaacaat
     9721 tgaatataac cctcaaatat aggagaggta atctgtgatt ttaaaaactg actgatgttt
     9781 tcaggaagtt cattcaacgg aatgctaatg cctgcatcat tcaaattacg gatgtcgtgc
     9841 ttcagcaatg aggcataagg gtgagcaaga taatttccta atgcaaaggt tgaaacaaac
     9901 ttctgctttg aagtttctga aatttgattg agtaaaacat ttagtgtttc aataaaacga
     9961 tacaaatgag tttctttaag cgggaatccc attgtgatat ttacatgctc aatctcttcg
    10021 ggtaaagatt gcaacaacaa aggcagtaat ttttcatcag gtaaaacaat agctgtctta
    10081 tcagtattga gtttattctg agcaatcaag gtttttaaac gatcagccag aaatttaaat
    10141 tgtcctgtaa tgagaggcac tcctgttaca ttaatgttct taggtatatc tttgaaatag
    10201 gtttcttgcc atttaaaact attctgagtt agaaaatttt ttctgaagaa aagcccggct
    10261 tcctgagttg tattttttgt gtaatactca tcggcatccc agaatatttt tccttttcca
    10321 tttaagatgt attcaaataa tttttgctct gcaggtgcca gtgcataaaa tccggcaata
    10381 atagtcttac tgcaatccag attttttaaa tatgtttcag gcgacaatgc taattgttgc
    10441 attgctttgc cggctgtagt cttattgttc ttatataaaa cctcattcag tttttcatat
    10501 aacttcggca atgcctgcca ggcatataaa aagttttgtt ttatagtaga agttttatct
    10561 gtactgaaca attcaaaaaa ctgtttcaac tgttctttaa attcatcatc atcctgaaaa
    10621 accgattcta tttcatgata tgatgtcagg tcagcaaaca ggtctttgta attaactaag
    10681 taaagattga tttcttcaaa atctccaaga atcatttcac cccaaggtgc aaaggaagta
    10741 aagtcataat cagggaaaaa tggcttgtaa acagcaaata aatcaagaag aacttctttc
    10801 ttttcagcaa ctcgcagtgg catgtaatgt tcaacaaaat cgctaataga taaaactgaa
    10861 ggcaataaaa cagctttgtt taattttttt gcgagttgca atttaaaaag caatgcagct
    10921 cttctggtag gaaatacaac agtatagtct gcaaaattat ttccatcaac tgtggcaata
    10981 agttcatcaa caatacgtgt taaaaatgaa tcagttttat tgttcatgac caacgctttc
    11041 ataaagcaga ctgctgtagc tgcgcgattt tgcattcaga tattttgctg cagcttctgt
    11101 taactgagaa gaagttactg cctcaatttt cttatatgta tcttctaagg taaatacttt
    11161 cttaaagttt aataaactgc gggcattaga aataaccaca ttcgacttgt tttcttttgc
    11221 caaagctagt tgacctttta gttgctgttt gtattgacta agttgtaatg atcctatttt
    11281 attatcagta agttttttca tttccaccac ggcaaggtca atacttttat tcaggaattt
    11341 tttatcggta gccagataga catgaaacat acctgtgtct gtataggagt taaaacctga
    11401 ttcaatggca taagtataac cgtatttttc acgaacacct aagttgagtt ttgagttcat
    11461 acccggccca ccaagaatat tattcaacaa ggcaacagtc attctgtctg cctgatagag
    11521 tgaaggtgct aatgtcccca taataaaatg acattgtgca ctgtgttttt taacagatga
    11581 cttttcagca gtgtattttt taaatggttt tggtctttcg cccactttgg caagtctgta
    11641 cttgccgaaa tgatgttcta attgcctgat tactttttct gcatttaatg atgtggcaat
    11701 tgatagcacc atgttggatg gaatgtagaa ctttcgtgta aacgcaatca ggtctttctg
    11761 cttaaaactt tttactgatt gttctgtacc taagattgga tttcctaatg gatttttttt
    11821 aaaaactaag ctttcaaagt catcaaaaat ctgctctccc ggattgtcga gataagacct
    11881 tatttcatca caaacaactt ccttttcttt ttctatttct tttaaattga aaacagaatt
    11941 aaaggtgata tcactaataa gttcaagtgc acgttcaaga tgaggtgtgg taactgatgc
    12001 atgaatacat gtctcttcct tgctggtgaa tgcatttagc tcaccaccta cgacttcaag
    12061 acgatttagg atatgaaacg atttacgctt tcgtgttccc ttgaacagca tgtgctctat
    12121 gaaatgcgct aatccttctt gttgtatttg ctcatttctt gttccggctt ttatgagtaa
    12181 tgcacaatgc gagacttctg tgttacttaa agttgtaaga actatctgta gtccgtttga
    12241 tagtgcaaaa gtttttgttg aatgatccat agtttatgca aaaatatgct ttgaaagtca
    12301 gacaacgatt ggctgattac atatctttac ctgataaaac aaaagtaact gttatgcaac
    12361 agcaaaaatg cctgaatttt tttattttat tcattttaag ttgtctgttt gcttctgata
    12421 ttgaggcgca gcaatataag tttgccaaac gtgtaggagg aacacaattc gagaatgcaa
    12481 tggggattgc attagatagc acaggaaata ttattgtgtc aggttatttt tcaggaactg
    12541 cagattttga tccgagcaca agcaattatt ctttaacaag caatggtggt aatgacattt
    12601 ttcttgctag atatgatagt gcaggtaatt ttatatgggc aattaatgta ggtggagcag
    12661 gagaggagtt taattactca gaccctgctg ttgatgagta tggcaacatt tatttatgtg
    12721 gtgtgtttaa caccaataca aattttaatc ctaagggaat tgctgtgatg gctacaaaca
    12781 gaggtcagca agatggtttt attgcaaagt atgattcaac aggattgttg caatgggtga
    12841 gaggtgttgg tggtgtattg aacgatgatg tttaccgaat tgactacaaa gattatttgg
    12901 ttttgtttgc aggctcattt actgattctt cttttgttga tgatggattg actgttactc
    12961 caatttatgg aagtggaaat gcagatgtgg tttttggaaa atttaatccg aatggaaatt
    13021 tgttttggct caatacgctt aaaggttccg gagaagatca ttcctataac attacagcag
    13081 gcatcaacgg caaaacatat cttacaggta cgtttgaaga cacattgatt tttgattatg
    13141 tctcagtgag tcccgataca ctctttgctg acggactagc agcattcact gcttcattta
    13201 gtaacatggg aaactactat tggtcttttc caatagagaa tgcttatcca tttggattga
    13261 aggttgatca taacggagac attctgacgt gtggacagtt ttcaggcatt gcagattttg
    13321 atcctgatgc tgatacaatg gcattgatgg cacaaggtag ctttgacgga tattttgcaa
    13381 aatatacgaa tggtggcagt tatgattttg ctaaaagaat aggtggcaat ggtgctgata
    13441 tttgttatac cattgccgaa ctttcaaata gctcaatttt ggtgagtggt tattttttta
    13501 atactgccga ctttgaccct gatgttactg ttgcatcact aaatagtaat ggctttgctg
    13561 atatgtatct tgcacattat gactctatgg gaaattatct ttcagctttt ggttgtggca
    13621 gtcctgcctt tgagttttgc cgcaacatga cctgtaacgg cattgacgaa gtgtttttat
    13681 gcggtggctt tgagcaggtt gttgacttta atcctgcacc ggctataaac acattggtaa
    13741 gtgccggctc gcgcgatggt tactttgcta aatatgcatt tccaacaacc tcattgaata
    13801 gtattagtgc agacaatctg atgatatatc ctaatccttt cattagtcag ataaatattt
    13861 caggagacat taatcaaaca caactaattt tgactgatgt attgggcaat actatttaca
    13921 aaacaaaaac caactctcaa acaacaatca atacagagaa attaaatccc ggaatttatt
    13981 ttctgactgt tgtatcaggt gaaaatgtga gtacgagaaa actattgaag gtgaagtgat
    14041 attagattgt aaggaaatta gataattaga taatttgaaa atgtggtagg agttttgaga
    14101 gttgcaatgt tggaaagctt ggaatgttgg aaagcttgga atgttggaat ttcttcagag
    14161 tgaccagata taaagttgtg tagattttga gggggtaaaa ggaaggcaaa attcaaaaat
    14221 aatttcctct tcaaaagaaa atttagctag tttcctgagt ggagggcttt acataggaac
    14281 ccgtgttacc agctgctcaa ttctagtcaa ggttctgtag ggttttcata ttactgtttc
    14341 ataaactttt ttatcggttg agaatagttt ttgagtcgta caaaaaataa gccgtttggc
    14401 aaatcagaaa tatctaattg agtagatgtt gttacagaaa attctttgaa caacattccc
    14461 attgaattaa aaatttgtat ttgagttttt tgattcgggt caaaattaat tgttaagtat
    14521 tctcttgctg gattcgggta aatagaaaat gtattttcag aaataggctc atttaaaccc
    14581 gtagaagaat tacacaataa cccttttgca ataaggtcat tgtttgcttg ttggatagtt
    14641 gaagcaagtg aagtatctgt tatgtcactt ccataaattt ccaaatagcg cattcctaat
    14701 gctaatccgt tatttaccgc agaatctaaa accatcgctt tagtatttgg taatattcca
    14761 cattgattca ttcgtgatgg cccatcctga acactccaaa gcatttgtgc tccggttcta
    14821 cagtgatttt gttgcatgag ataccaatga gatgtatttg ctatattgga caaattgggg
    14881 ttgcagcctg agacatcttc tctaaataaa ccaaacctgt ccgggtagtt tatgatacca
    14941 taattgcaat atattgaagc caaataattt ctggcttgtc cacttgcatt aggctgaaaa
    15001 gtaacataat caacagaaca ccatagtggg gtggaaggga acacactcat atagtagtca
    15061 gtcatttcat ttattaatgc acctaagctg tcagcattat aattatatac agaccaaaat
    15121 gcttgtttag ttatggttac ggtatcggtt agaactgtgt caggtaagtt tctagaaaaa
    15181 ttaacaccga tcacattcac atacgttacc acaggattgc ccgcatattt tatagcaagg
    15241 ttttgcaaaa agattttgaa tctgcttaca taaacactgt cccatggaat aactcctgtt
    15301 gttatttgtc caaaattagg actcgatgga ttgtcttgaa tagtataata ctgctgtgct
    15361 ccgatactgc caagccagtt gggtattcct aagggttgaa gagatatttt tttattgtaa
    15421 gtaactgctt ttgcgatttc tccatcaatg aatgcccaat tgaaattgcc cggactaggc
    15481 tccaaatcat tccatctaaa acgcactaca acaccgctaa tattggggtc attaaaactt
    15541 gttgtgctaa tcggcatatt ggctgtacct aaaaaataca cgccattatg gccaataaat
    15601 tgagcttgtg tatctaaaac aacaaacaaa cttatcaggg ttaaaaataa cctcattttt
    15661 gaatgataat tttatgatgg aacatttttc gatatagatt gattgtaata aaataaatac
    15721 cattgttcaa ttgtgaaaca tttatttgtt cattgggcat aactatttta gataacaata
    15781 attttccgct cacatcaaac aaacttacat caattctttt ttcagttttg aattggataa
    15841 aatcagatgt cggattaggg aatatggaaa tcatttcaga agatatctgt tgattaattg
    15901 atgttgtgtt tgcacaaccg tgcaatgcaa accattgttg ggttctggca aacattagac
    15961 tatccatact gattgaactg tgggtatatc cactgtattg ataaaattga tgcggaattc
    16021 ctgtcgaatt aaatacgctg ttgagcaaac tatccatctc tattggaact gtgttatctg
    16081 catcgccatg atgtataata tacgggcagc ttatctgagc tgcaagcatt gtagtgggtg
    16141 cagaaaaccc caattgggga gaaacgcctc ctgctgtatg tccagccgct ctgaatttat
    16201 cagggtatgt ggcaactaaa cctgttgtaa ggtatgcacc tctgctatgt ccaaatgcca
    16261 taatacaatt cgtatccaca taatttaatg atgccagaat atcccagcat ttcatgtcac
    16321 ggagatagtt attggtgctg gcaccaaaat ttatcaagct tgaatcgcca ggtgaaccga
    16381 taggtgcacc ggcagaatga cataaattaa cagctatgca agtgtaattc cattgaacca
    16441 ttttcttggc tacattttta ctgtatccgt tcacattccc ccccgtacca tgattaatga
    16501 taacagcagg aaaaacacca gagcccacag gcttaaacaa aatcccggta aggtcgtaag
    16561 acgtaccatt aatagtctgc tgataagtcc aagtagcgcc atttaaactt tcgggatttc
    16621 ctgtcaaggt aaggcccgtt agttgtgcca ttccacttga tgaaatgaaa agtccgagaa
    16681 taagtaaaag tcgtttcatt tttatttatt gtattgcatc tgtgttcatt ttcagggttg
    16741 cttgtaattt cagtctttcc gataactatc ggaattaaat ctattcatta gttcaaaagt
    16801 aaatagaaaa atgatttaag gaggcaatag cagcaaatta ggctgtatta aaattaattg
    16861 cttgtattaa atcacactgt caggggctgt gcgacctgag gtgaaattag ctgtgttcta
    16921 ttttcataaa aaacgtatta actcttcaat gtttttttat tatttcaaag tcccctgaaa
    16981 tgcgggagac tttgaaagta cggagataaa agagattgtt tgaataaagc aatctctttt
    17041 tggtttttga agagagtcta ccttctccat attggttaca tataaatcac ttttacttta
    17101 atttctttgt cttgaaataa atcaaaactt gcttttttaa aatttgggcg gttggttaaa
    17161 ccgatagatg aataattaga aaagccaatt ccttcttttg ggaaaattaa acctttgtca
    17221 atcttgccgt tattgttttc atcgtgtaaa atgtttaccg catatttccc ttttggtagg
    17281 tttttaaata tgattttact tttaccattt gtaatttggg cagtttctat tttaaaataa
    17341 tttttgtaat actcatctgg tattgagccg tctttgttat ataaagctac ttgtaaaatg
    17401 ccttttgaat tacgtaaact ttcaacttct attgtcaaac tatattcttc tgctttagcc
    17461 tgagcaaaaa cacttaaaac aaacactgac ataacaacaa aaaatactac tctcatattc
    17521 ttaattcata acttttaatt cgtagttcgt aattttaaat tcttaattct taatttttaa
    17581 tttctccttc actctatcca ctccgtaata caccattggc actaagaata cagtcaatat
    17641 taatgaagaa agtaagcctc caataattac ccaagccaag ccatttttcc attctgctga
    17701 tgttcctgtg gctaatgcaa tgggtaacat tccaattgcc attgataaag ttgtcattaa
    17761 aattggccgc attctgcctt tgcttgcttc gataatggct tgacgatagg gtttcccttc
    17821 ggcttttaat tgattagtaa aatcaacaat taaaattgag tttttggtta ctaatccca
//
LOCUS       DAORQT010000012        17530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_269_length_17530_cov_13.979170, whole genome shotgun sequence.
ACCESSION   DAORQT010000012 DAORQT010000000
VERSION     DAORQT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17530
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_17530_cov_13.979170"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1821)
                     /locus_tag="PK134_00995"
     CDS             complement(112..1821)
                     /locus_tag="PK134_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRB37461.1"
                     /translation="MKAFHFLKYNESTSIDKLIAQSAPETVLCFDLEDSIQDCLDSGK
                     TTVLKKIFREYFRSMLVACKTDDGKLNIGVRINSTEGMEYNLDLLSLSGTGQISTVFL
                     PKVESAQQILKLQNDLQENNISFDEIIPVIETKVGLKNLSSILKSDLKKVKRFAFGHC
                     DYNNDCGNFPFFHQDSREYWTWITKIFKELKLYGYTIVNSPCLQLDNEVYFLNMLNIL
                     VSICGSDCAQVTLTKTQTEVCVSYSVIKPDAELPLMGNRLDFKVPAVYIESFIKEFEN
                     KYHEKGFVISNGRVLISPQEYVAALNYRKKANLPEINFTFLGGCFPVQGNMPFENRFH
                     QLLKDKVENCRNVKFNINIIRYERFKNCFNKIETYNKSHPIDVLVVSIRPEPVLRLIK
                     LYYKFKKNPGGRIERSLNIPLLNKIDAESYDLLSASTRFNPVENTANSKMHSALVDLN
                     YFFGMLIGNYSYALEKYKELLKDIIKFTNNRNIQLIVLGPPERTNALFEKLITLRLEK
                     DIKKYLEYIPECFVSGRSQNYKGEILFDSLGVYATKAYHLMIAQALYEKILPNIDDIN
                     IRK"
     gene            complement(2262..3641)
                     /locus_tag="PK134_01000"
     CDS             complement(2262..3641)
                     /locus_tag="PK134_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRB37462.1"
                     /translation="MKRNIVVVIIFLLLFGAQSCRKAEVLSESEWNEWLAGGKQTLFD
                     ESAGAFSSVFPDISAHNAMIHEMGDKAFEATFVSAPAPLNPGLGPIFNNVSCASCHIA
                     DGRGKPPLNSSEPLSAMLIRVSIPGVASNGGPLAVPGFGVQLQQRSINGVAKEADVII
                     NYSEQTFSFPDGETYSLRTPTYQLANSYIPLPAGVMLSPRMAPPVFGLGLLEAVDESE
                     ILKFADENDANGDGISGKPNYVWNVLAGTRTLGRFGWKANNPTVLQQTAGAYNEDMGI
                     TSYVFPAESSKGQTQYDNRNDDPEIPDSVLRAVSFYVQTLAVPVRRSADDATVLQGKK
                     LFNDAKCGSCHRPAMQTGVRVDFPEVSNQRIFPYTDLLLHDMGEGLADHRPDFDANGN
                     EWRTAPLWGIGLTQKVNGHNFYLHDGRARSLQEAILWHGGEAESAKNAFKNMSKSDRQ
                     ALIKFLQSL"
     gene            complement(3644..4693)
                     /locus_tag="PK134_01005"
     CDS             complement(3644..4693)
                     /locus_tag="PK134_01005"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRB37463.1"
                     /translation="MQFRFILFSLASVLVLSSCKKDKSNSDDYSSTQILNDFSVNVAQ
                     ATYNDLASKTEQLHQAITNFTQNQTQANLDNCKSLWKQSRQAWEQSEGFLFGPVATDD
                     IDPRIDTWPVDFNSLDSVLNNQSVYDEAYVNSLEDALKGFHPVEYLLWGVDGNKTIAQ
                     FTSRQLDYLEALAANLKTLTQQVASSWTTGNYHNEFANAGNGSQVFTTQHAAFMEMVS
                     AMAGICEEVADGKMEDPLVSQNAMLEESPFAKNSIIDFTNNIKSVENVYLGKYTSQGQ
                     SLESFVRANNLSLDGSIKTKISAAITALGNITVPFGQAIFTQQTQVINAQNAIRDLKS
                     VLENDLANLVSQKIQ"
     gene            complement(4701..5123)
                     /locus_tag="PK134_01010"
     CDS             complement(4701..5123)
                     /locus_tag="PK134_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRB37464.1"
                     /translation="MKRRNFIKNTCMSCGMMAAGGSLLSLLSSCKSTSIFKAEAVNHQ
                     LTVPLSAMNDKKILIVRNASSEYDILLVKKPDNHFTALEMKCTHRANPLNASDGGLYC
                     TLHGSRFDLDGNVVAEPATQPLKKYPVVVNNSLIQISI"
     gene            complement(5126..6313)
                     /locus_tag="PK134_01015"
     CDS             complement(5126..6313)
                     /locus_tag="PK134_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37465.1"
                     /translation="MKNYITVILFLFPFLSQAQTGEDSLHTNAANEKQTYISGYGEAM
                     VSYDSRYRTAQANFTRAVVFVGHKFSKNISFLSETELADARVEGGKAGGEIALEQAYL
                     RFQLNRKMHLQAGLFIPRIGIINENHLPTTYHGNQRPFTETFLIPSTWRELGVALYGS
                     SDAITGLNYSVALVNGLDASGFSNGTGIRGGRFEGGNATASNIALTGSVQYVMKNFTF
                     QLSAYYGGSAGLTKREADSLQLNSGMFGTPVGLGEVNVQYRKDRLTAKVLFAAITIPD
                     ADKINRAYANNTAELSVGGYAEVAYRILKKLKTDAFVRYENFDLNSSIPKNGIKNDIL
                     KQQYIIAGFSCFPTPGVVVKLDYVYKQTGDLNPALNLNPFPNAPAYYTTNSFINLGIG
                     YSF"
     gene            complement(6368..7939)
                     /locus_tag="PK134_01020"
     CDS             complement(6368..7939)
                     /locus_tag="PK134_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB37466.1"
                     /translation="MMTGRLQSFSKPLFFAGVLLFAVGLPLSMFLMSVSQALIIVAWL
                     LNGDLKEKLKTALSNKIVLLLVVFFLWHLLGLLYTTDFHYGFKDVRVKLPLLLIPIIF
                     SSMPVFSDKQFKTVALVFIASVLVSTLISMCIYVGIIPVEFHNVRDISIFISHIRLSL
                     LICIAVYLSFWLLRKIKFSYGLLLVIIWFVFFLSLIESVTGLGILAIVLLIYAIKTFL
                     KNKSVVSRGIIAGSVVMVVTILTFTIKSAWHDVTFFNDAELRNLAKHTANGAPYTHDT
                     LNRESENGHPVWINICEPELRTAWNKRSKINYDDKNLNGDEIKLTIFRFLSSKGLTKD
                     AHAVEQLSNTEINAIEHGVANAHDLIDSNIHNRLRATLWELNRYRNGGNASGHSLTMR
                     FEFWKTALQIIKQHPLTGVGTGDVPDAFVKKYDETNSLLDAKFRLRSHNQFLAVAVAL
                     GIPALLLFIVMLFYPVFQFQYSGSFLYLTFWITAFLSMLTEDTLETQAGVSFFIFFNC
                     LFLFLKDKELDNNFI"
     gene            complement(7952..9628)
                     /locus_tag="PK134_01025"
     CDS             complement(7952..9628)
                     /locus_tag="PK134_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRB37467.1"
                     /translation="MRKRIRLYVAVILVGISAFSVYSFSDDYFEISRNLDIFATLFRE
                     LNIYYVDETNPGDLMKKGIDDMLASLDPYTNYIPESEIEDYRFMTTGQYGGIGSLIGS
                     RNNEVIITDPYEGFPAQKADLRAGDVIVELDGKPIKGKKNDEVSKLLKGQPKTSVKIT
                     IRREGEPNLIDKNVVREEIKINSVSYSGIIDNDLGYIRLTGFTENAGQEVKNALQALE
                     AKTKLKGLIFDLRGNPGGLLNEAVNIVNIFVNKGVEVVSTKGKAKEWDKTYHALNNPV
                     DTEIPVAVLVNSSSASASEIVSGSLQDLDRGIIIGQRTFGKGLVQTTRPLSYGAQLKV
                     TTAKYYIPSGRCIQALDYSHRNDDGSVGKIPDSLITQFKTKSGRTVYDGGGVMPDFVT
                     DVRQLSPISQSLLLKYLLFDYATMYRNTHPSIAPAKEFHLSDAEYAEFVKWISAKDYD
                     YETKSEKILDDFKTTAEKEKYFSAAAAEFEVLKKKISHDKNADLQKFKDEITELLENE
                     IVSRYYYQSGQIEASFKNDLEIKKATEALKNKDVYSSIMKSSMASVREKH"
     gene            complement(9680..9901)
                     /gene="yidD"
                     /locus_tag="PK134_01030"
     CDS             complement(9680..9901)
                     /gene="yidD"
                     /locus_tag="PK134_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRB37468.1"
                     /translation="MIKLLNRILIAIVKFYKMVISPLLPMSCRYYPTCSEFCMEALKK
                     YGPLKGGFLSLKRILSCNPFGGHGHHPVP"
     gene            complement(9852..10292)
                     /gene="rnpA"
                     /locus_tag="PK134_01035"
     CDS             complement(9852..10292)
                     /gene="rnpA"
                     /locus_tag="PK134_01035"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRB37469.1"
                     /translation="MNEGTLRNTFSKAERLCKRKDFELIFSQGAAFNRFPFRVHWLKL
                     KENTSTVPVKIAISVPKRKIRSAVSRNRIKRLIRESYRLNKQILSNELKKNEQVIHIM
                     FVYNGNINATFTEIQSKIILILQRLKELNDQTAEQDSDSHSKVL"
     gene            10391..11008
                     /locus_tag="PK134_01040"
     CDS             10391..11008
                     /locus_tag="PK134_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37470.1"
                     /translation="MLKSGLALCLLFILSLGNINAKETIFKEQRIGFGYYPSFHGSQN
                     FWLNYSVNKRSRKTIYSIRGMYNFRSEKAQEVSNYAIGPCVSVKLLSFFKERLEIRPT
                     LYIPYVNFKYEKSTYPGSLYATQYNGAALLPGLNIQTELTDRIGVRYGANAGIYYAKT
                     REPIFEQKNGVTTYDVYTDKNMYWIMLIDFSITIKITKNYLPHQR"
     gene            complement(11019..11774)
                     /locus_tag="PK134_01045"
     CDS             complement(11019..11774)
                     /locus_tag="PK134_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37471.1"
                     /translation="MNPIITVNNLVKHYDSFHAVKGISFEVYEGEIFGLLGPNGAGKT
                     TTLEIMETLRAKTSGDITIKGNKVGTDDDAIKKIIGVQLQAAGYYPSLSLLELIDLFA
                     GLYSQDVNAMELLQKVGLTDKAKSKYKELSGGQKQRFSIATTLINKPAVIFLDEPTTG
                     LDPQARRNLWDLIRQIKNEGTTVVITTHYMDEAEELCSRVAIIENGKIIALDTPDQLI
                     DDLVAKGFERKKEVKKANLEDVFLNLTGQEWRE"
     gene            11849..12433
                     /locus_tag="PK134_01050"
     CDS             11849..12433
                     /locus_tag="PK134_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRB37472.1"
                     /translation="MLKVDDILVSDQVITEKFICDLSKCKGACCVEGESGAPLEEDEL
                     KALEKVYPKVKPYLTDEGIKAIEAQGFHLKDDDDEWVTPLMWPGGPCAYTVFENGIAL
                     CGIEKAYRDKKIKFYKPISCHLYPIRVLKTKSGDALNYHEWEVCSPACKLGKKEKVRV
                     YEFLKTALTRKYGEAWYNKLIKAAQEYLQTLTKK"
     gene            12488..14644
                     /locus_tag="PK134_01055"
     CDS             12488..14644
                     /locus_tag="PK134_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB37473.1"
                     /translation="MYLPKHVISWLFFLIIIQSATAQTTISGTVTDQKNNPVAGANIV
                     IRDSYDGASSDASGKYQFNANDSGDVIIVCSLIGFSPSDKKIKLQGNTLEINFKLKET
                     SNELNTVTITAGAFEASDEKKMVILRPLDIVTTAGSQGDIYGALQTLPGTQPTQDQEG
                     LFVRGGDAAEAKTLIDGIEVANPYFSSVPDVPQRSRFSPFMFKGTNFSTGGYSAQYGQ
                     AMSSVLALESEDLPPRSVGNIGIMSVGGGGGYMHRWKKTSAGAFANYFNLAPYFAIIK
                     QNRDWDSPPQTFSSSAVFRHKTSETGIIKAFINYQYSDLSLNFDNLEDVTGKSKNHFG
                     LNNYNIFSTLSYKEVLNKWTVQAAGSYSKDINRIYARPQHLLFSNQLWQGRAVVSRSI
                     SELSSIRGGVEVKYNDFSNLYNNFRVDSTEMFTAVFLEGDAYITRKLVARAGLRVENS
                     YLLRASNLAPRISVAYKLGKFDQLNIAYGDYYQTPDKNYLIFTNLDHFEKATHYILNY
                     QYVNEKRTFRVEAYYKKYNHLVQTTNMASDVYATAFATGDDGKGYAQGIDFFWRDKQT
                     FKYVDYWISYSFLDTKRKYLYYPFEVQPTFASKHVVSVVMKRWFPKLRSSIGFTYVYA
                     AGRPYYNPNKTNENNFLSDRTNDYNNLSLTWSYLTSIGRHFTVISAAVNNVLGIESAY
                     SYRYSTDGLRRSAVGPTSKRSFFIGVFISFGEDRTE"
     gene            14668..15306
                     /locus_tag="PK134_01060"
     CDS             14668..15306
                     /locus_tag="PK134_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37474.1"
                     /translation="MKKLTFLLLFIAGNMFAQSNSKFEKTMVKQIDLLDKAKAGTDFV
                     NAANAFERIANAETKEWLPLYYQAYALVLSSNDEKDNVKKDAVLDKALEIANKADKLS
                     PDNSEIVTLQSWITSMKIGIDPATRGQKLGMEAGMLTAHAIELNPENPRPYFLQGTGL
                     LFTPEQFGGGKAKALPVLEKAIEKFNAFKPANAMMPNWGKEFAQKMLDECKK"
     gene            complement(15438..16073)
                     /locus_tag="PK134_01065"
     CDS             complement(15438..16073)
                     /locus_tag="PK134_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB37475.1"
                     /translation="MQIKKFTFNPFEENTYIVYDETAQCAIIDPGCYMPEEKEELLSF
                     IAEQKLNPVLLLNTHCHVDHIFGNKLIYDTFGLKPQFHQLELRLLEAAGEYSKQWGIF
                     LEPSPTAEKYLEEGDIISFGNISLEILLTPGHSPGSLCFYDKQEKIIIGGDVLFQMSI
                     GRYDLPGGDEQTLLNSIRTKLFTLPADVKVLPGHGPETTIGFEKMYNPFLV"
     gene            16668..17264
                     /locus_tag="PK134_01070"
     CDS             16668..17264
                     /locus_tag="PK134_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRB37476.1"
                     /translation="MEYIKHLSKDKKLASVIKQHGTVELKKRKDLFLSLCGSIISQQL
                     SVKVADVIKQRFYDLFDGKPTPEKILQATTETLRSIGLSNSKASYIKNIAAFALENSL
                     QLNKVNKMSNDEFIEYITQIKGVGRWTAEMIMMFTLGREDVFSVDDYGIQQSVIGLYK
                     IKISDKKKLRLRILAIAEQWAPYRTYACMYLWRWRDNG"
BASE COUNT         5586 a         3275 c         3066 g         5603 t
ORIGIN      
        1 actaatgctt gaatgataga tgggcacaag tccgaagact tgcgccagag aggtttttat
       61 gttgttaata aattacatta agctggcagg tatcaaatgg tgcgcagttg gttatttgcg
      121 aatattgatg tcatcaatat taggcaatat tttttcatat agtgcttgag caatcatcaa
      181 atgatatgct tttgttgcat aaacaccaag gctatcaaac aaaatttcgc ctttataatt
      241 ttgacttctt ccacttacaa aacactcagg aatatattcc agatattttt ttatatcctt
      301 ttccaatctt aatgtaatca gtttttcgaa tagtgcattt gttctttcgg gcggacctaa
      361 tacaatgagt tgtatatttc tattattggt gaattttata atatctttca aaagctcttt
      421 atacttttct aaagcataac tataatttcc gataagcata ccaaagaagt aatttaaatc
      481 aaccaatgcg ctatgcatct tagagtttgc tgtattctcc accggattaa atcttgtaga
      541 tgcagaaagt aaatcataac tttctgcatc aattttattc aataaaggaa tattcaatga
      601 tctttcaatt cttccacctg gatttttctt aaacttgtaa tataacttaa tcaaacgcag
      661 caccggttca ggccttatag aaacaacaag tacatcaatt ggatgcgact tattataagt
      721 ttctatctta ttaaaacaat ttttaaatcg ttcatacctt attatattga tattgaactt
      781 tacatttctg caattctcaa ccttatcttt tagcaattga tggaaacgat tttcaaatgg
      841 catatttccc tgcacaggaa aacaaccgcc aagaaatgtg aaatttattt caggtaagtt
      901 agcctttttg cgatagttta atgcagcaac atactcttgt ggagaaatta atactctgcc
      961 attacttata acaaagcctt tttcatgata cttattttca aattccttaa taaaactttc
     1021 aatataaact gctggcactt tgaaatctaa tctatttccc attaaaggta gctcagcatc
     1081 aggttttatt acggaatatg aaacacaaac ttcggtctgc gtttttgtta aagtaacctg
     1141 tgcacagtct gaaccacata ttgaaactaa aatattaagc atattcaaaa aatacacttc
     1201 attatcaagt tgtaaacatg gagaattcac aatggtataa ccgtacaatt ttaattcttt
     1261 aaaaattttg gttatccatg tccagtactc tcttgagtct tgatgaaaaa aaggaaaatt
     1321 accacaatca ttattataat cacaatgacc aaaagcaaaa cgctttactt ttttaaggtc
     1381 actctttaaa attgatgaaa gatttttcaa accaactttg gtttctataa caggaattat
     1441 ttcatcaaag cttatgttgt tttcctgtaa atcattttgc aatttcaaaa tttgttgcgc
     1501 actctcaact tttggaagaa acactgttga aatttgtcca gttcctgaaa gtgacaaaag
     1561 gtctaaatta tattccatgc cttctgttga atttatgcgt acaccaatat tcaacttacc
     1621 atcatctgtt ttgcatgcaa caagcatact tctgaaatat tctctaaata ttttttttaa
     1681 gactgttgtc tttccggagt ctaaacaatc ctgaatactg tcttcaaggt caaaacataa
     1741 aacagtttcc ggtgccgact gcgcaatcaa tttgtctatt gatgtacttt cattatactt
     1801 taaaaaatga aatgctttca tgatcagtcg ttgctggaat aatattagaa attattcctt
     1861 ccacaaactt aataaaaagg tcataaagga aattaaacta tgacattaaa gtatattcct
     1921 tcttgttgga taaattaaac taacactaaa ctacgcttaa cctttcttgt ttttttgcaa
     1981 taaccggaaa tgataaataa tagaaataat acctatgata attgatgcaa tgcctgcgtt
     2041 tttggaagca tcagtaaaaa ctctaaaata agatagaatg cctactatta aaaaaccgaa
     2101 acctgcaatc aaagaaagat acttattgta aaatatcata actcaatctt ataaatacag
     2161 catcaaaagt aaatcgaaac aaaacacttt tgatttattt taaaaattcc tgagcatcca
     2221 tcatcatttc atcacagtca ggaaaattct gaaagaatga attataatga ctgcaaaaat
     2281 tttatcaatg cttgtctgtc gcttttgctc atatttttaa aagcattctt tgccgattct
     2341 gcttcaccgc catgccataa aattgcttcc tgcagcgaac gtgctctgcc atcgtgcaaa
     2401 taaaaattat gaccgtttac cttctgtgtt aaacctatac cccataacgg tgcagtacgc
     2461 cattcattac cattggcatc aaaatcaggt cggtggtctg caagaccttc gcccatgtca
     2521 tgcaacaaca aatctgtgta aggaaaaatt ctttgattac ttacttccgg aaaatcaaca
     2581 cgaacaccgg tttgcatagc aggtcggtgg caagagccac acttggcatc attaaatagt
     2641 tttttgcctt gcaacactgt ggcatcatca gcacttcttc ttacaggcac agctaatgtt
     2701 tgcacataaa atgaaactgc acgcaaaacg ctatcaggaa tttcagggtc atcgtttctg
     2761 ttatcgtact gtgtttgccc tttgcttgac tctgccggaa aaacataact tgtaataccc
     2821 atgtcttcat tataggcacc tgctgtttgt tgcaacactg ttggattgtt tgccttccat
     2881 ccaaagcgac ctaaagtgcg tgttcctgca agtacattcc aaacataatt cggcttgccg
     2941 gaaatgccat caccgttggc atcattttca tcagcaaatt ttaatatttc agattcgtca
     3001 actgcttcga gcaaacccaa accaaataca ggaggtgcca ttcgtggtga aagcattact
     3061 cccgcaggta atggtatgta gctgttggct aactgatatg ttggtgtacg caatgaatat
     3121 gtttcaccgt caggaaaaga aaatgtttgt tcagaataat taatgataac atctgcttct
     3181 ttcgcaacac cattaataga tcgttgttgc aactgcacac caaaacccgg cacagctaat
     3241 ggtccaccgt ttgatgccac accgggtatg cttactctga ttaacattgc agagagtggc
     3301 tcactgctat tcaacggagg cttacctctg ccatctgcaa tatgacatga tgcacaggaa
     3361 acattgttga agataggtcc aagtccggga ttcaatggtg caggtgcact gacaaatgtt
     3421 gcttcaaaag ctttgtcacc catttcatga atcattgcat tgtgtgctga aatatcagga
     3481 aacacactgg agaatgctcc cgcagactcg tcaaaaagtg tttgtttacc tcctgcaagc
     3541 cattcattcc actctgattc tgacagcact tcggcctttc tgcaagactg tgctccgaat
     3601 aaaagcagaa aaataattac aacaacaatg ttccgtttca agattattga attttctgac
     3661 taactaaatt tgctaaatca ttttctaaaa cacttttcaa atcacgaata gcattctgtg
     3721 cattgataac ctgtgtttgt tgcgtaaata tggcctgacc aaaaggaact gtaatatttc
     3781 ctaatgcagt tatagcagca ctgattttcg ttttaataga accatctaaa gaaaggttgt
     3841 tggctctgac aaagctctct aaagattgcc cctgagaagt atattttccc agataaacat
     3901 tctctacact ttttatgttg ttcgtaaaat caataattga attttttgca aaaggcgatt
     3961 cttcaagcat agcattttgt gaaactaatg gatcttccat ctttccatct gctacttctt
     4021 cgcaaatacc tgccatagca ctaaccattt ccataaatgc cgcatgctgt gtagtaaaaa
     4081 cctgactgcc atttcctgca tttgcaaact cattatgata gtttcctgta gtccaacttg
     4141 aagctacttg ctgcgttagt gttttcagat ttgctgcaag tgcttctaag taatctaact
     4201 gacgtgaagt aaactgtgca atagttttat ttccatcaac accccacaac aaatactcaa
     4261 caggatgaaa tccttttaaa gcatcttcaa gtgaattgac ataggcttca tcataaacag
     4321 attgattatt caaaactgaa tcgagtgaat taaaatctac aggccaggtg tcaatacgtg
     4381 ggtctatatc gtctgttgca acagggccga ataaaaatcc ttcggactgc tcccatgctt
     4441 ggcggctttg tttccataac gatttacagt tatcaagatt tgcctgtgtc tgattttggg
     4501 taaagtttgt aattgcctga tgcaactgct ctgttttaga ggctaagtca ttataagtag
     4561 cctgtgcaac attaactgaa aagtcattaa gaatttgtgt tgaagaatag tcatcactgt
     4621 tgcttttatc ttttttgcag gaacttaaaa ccaacactga tgctaatgag aataaaataa
     4681 atctgaattg catgattaaa ttaaattgat atttgaatta gtgaattgtt tactactaca
     4741 ggatattttt tgagtggttg tgttgcaggt tcggctacaa catttccgtc aaggtcgaag
     4801 cggctgccat gaagtgtgca gtacaagccg ccatcacttg cattgagtgg gttggcgcga
     4861 tgtgtgcatt tcatctctaa ggcagtaaaa tgattgtcag gtttttttac caataagata
     4921 tcatactctg atgaagcatt acgcacaatt aaaatctttt tatcgttcat tgctgacagt
     4981 ggaactgtca gttgatgatt cacggcttct gctttgaata ttgacgtgct cttacaagaa
     5041 gaaagcagcg acagtaaact tcctcctgct gccatcatgc cacaactcat acatgtgttc
     5101 tttataaaat tcctgcgttt catttttaaa aactatagcc tatgccaaga tttataaaac
     5161 tgtttgttgt atagtatgca ggtgcattcg gaaatggatt caggtttaat gcaggattaa
     5221 gatcaccggt ttgcttatat acgtaatcta attttaccac tacaccgggt gtaggaaaac
     5281 aagaaaatcc tgcaatgata tattgctgtt tcagaatatc gttctttatt ccattttttg
     5341 gaatactgct gttaagatca aaattttcat aacggacaaa agcatctgtt tttagttttt
     5401 tgagaatgcg ataagcaact tctgcataac caccaacaga taattcggct gtgttgttgg
     5461 cataggcacg attaatttta tcagcatcag gaatagtgat tgcagcaaaa agcacttttg
     5521 ctgtaagtct gtctttgcga tactgaacat ttacttcgcc aagccccaca ggggtgccaa
     5581 acatgcctga gtttaactga aggctatcag cttctctctt tgtcaggcct gcgcttccac
     5641 cataataagc tgaaagctga aaagtgaaat ttttcattac atattgcact gatcctgtca
     5701 aagcaatatt agaagcagtt gcattgccac cctcaaacct acctccgcga ataccagttc
     5761 cattgctgaa gcccgatgca tcaagaccgt tgacaagtgc aacagaataa tttaatcctg
     5821 ttatagcatc tgaactacca tacaaggcaa cacccaactc gcgccatgtt gatggtatta
     5881 gaaaagtttc tgtaaagggg cgttgattac catgataagt tgttggtaaa tggttctcat
     5941 taataattcc aatgcgcgga ataaataatc cggcctgcaa atgcattttt ctgtttagct
     6001 gaaaacgcag ataagcttgc tccaatgcta tttctccacc tgccttgcca ccttcaacac
     6061 gtgcatcggc caattctgtt tctgaaagaa aagaaatatt cttgctgaat ttatggccta
     6121 caaaaactac agctctggta aaatttgcct gagcagttct gtagcgtgaa tcataactta
     6181 ccatcgcttc gccatagcct gatatgtagg tctgcttttc attagctgca ttggtatgta
     6241 atgaatcctc acctgtctgt gcctgagaaa ggaatggaaa tagaaacaga atgactgtta
     6301 tgtagttttt cattgaatga aaattatcgg ctgcgaaagt aatgagaaaa tactatttag
     6361 aatgtttcta aataaaatta ttatctaact ctttgtcttt cagaaacaaa aacaggcaat
     6421 taaaaaaaat gaaaaaacta actccagctt gtgtttcaag tgtatcttct gttaacattg
     6481 acaaaaaagc cgtaatccag aaagtcagat ataagaaaga ccctgaatac tgaaattgaa
     6541 aaacaggata aaaaagcatg acgataaaaa gtaaaagagc gggaataccg agtgcaacag
     6601 caacagcaag aaactgatta tgcgaacgca aacggaattt agcatccaac aatgaattgg
     6661 tttcatcata cttttttaca aaagcatcag gcacatcacc cgtgccaaca cctgttaacg
     6721 gatgttgttt gatgatttgc aaggctgtct tccaaaattc aaaacgcatg gttaatgaat
     6781 gtccgctggc attgcctcca tttctatatc ggttcaactc ccaaagtgtt gcccttaatc
     6841 tgttgtggat attagaatcg attaaatcat gtgcattggc tacaccatgt tctattgcat
     6901 taatctcagt attactgagc tgttcaactg catgtgcatc ttttgttaat cctttggaag
     6961 ataaaaaccg gaatattgtt aatttaatct catcaccatt cagattttta tcatcataat
     7021 ttatcttgct tctcttgttc catgctgtcc gcaattccgg ctcacaaatg ttaatccaca
     7081 ccggatgtcc gttttccgat tcacgattca gagtgtcatg tgtatatgga gcaccatttg
     7141 ctgtatgttt tgctaaattt ctcaactcag catcattaaa aaatgtcaca tcatgccatg
     7201 cgcttttgat agtgaatgta agtattgtaa caaccattac aactgaacct gctataattc
     7261 ctctgctaac tacagattta ttctttagaa atgttttgat agcataaatc aataacacta
     7321 ttgccagtat gcccaaaccg gtgacagact caatcaatga aagaaaaaac acaaaccaga
     7381 taattacaag cagcaatcca taactaaact tgatttttct taacagccaa aatgacaaat
     7441 aaacagcaat acaaataagg agtgacaatc tgatgtgtga aataaaaatt gaaatatctc
     7501 tgacattgtg aaactcaaca ggtattattc ctacataaat acacatgctt attaatgtgg
     7561 acactaaaac ggatgcaata aaaactaatg ctactgtttt aaattgttta tcagaaaaaa
     7621 caggcattga tgaaaaaata attgggatta aaagcaaagg aagtttgaca cgcacatcct
     7681 taaagccata atgaaaatcg gtagtataaa gtaatcccaa cagatgccat aaaaagaaaa
     7741 caaccagcag taaaacaatt ttattagata atgctgtctt taatttctct ttcaaatctc
     7801 cattcaaaag ccatgcaaca atgatgaggg cttgagaaac actcatcaaa aacatggaga
     7861 gtggcaatcc aacagcaaac agtaaaacac cggcaaaaaa aagaggttta ctaaaacttt
     7921 gtagccggcc tgtcatcatt aatgcagtat tctagtgctt ctctctaacc gaagccatag
     7981 agcttttcat gattgaacta tagacatctt tattcttcaa tgcttctgta gccttcttaa
     8041 tttccaaatc gttcttaaaa gatgcttcaa tctgtcctga ctgataataa tagcgtgaca
     8101 caatttcatt ctccaacaac tctgttatct catctttaaa cttttgtaga tctgcattct
     8161 tatcatgcga tatttttttc ttcagcactt caaactctgc tgctgctgca ctaaaatatt
     8221 tttctttttc tgcagttgtt ttaaaatcat caagaatttt ttcgctcttg gtttcataat
     8281 cataatcctt tgcagaaatc cattttacaa actcagcata ttcagcatcg gagagatgaa
     8341 attcttttgc aggtgcaatt gatggatgtg tattacgata cattgttgca taatcaaaca
     8401 ataaatattt cagtaataaa ctttgcgaaa taggactcag ttgtctgacg tcagtaacaa
     8461 aatcaggcat aacaccaccg ccatcataaa cagtacggcc agacttcgtt ttaaattgtg
     8521 tgatgagtga atcaggaatt tttcctacgc tgccatcgtc attgcgatgt gaatagtcga
     8581 gtgcctgaat gcaacgacca ctgggaatat agtattttgc tgtggtaact ttaagttgtg
     8641 caccataact taacggtctg gtagtttgca caagtccttt accgaaagta cgctggccaa
     8701 taatgatgcc tctgtctaaa tcctgcaaac ttcctgaaac aatttctgat gcagatgcgc
     8761 tgctgctgtt tactaaaact gctacaggaa tttctgtgtc aacaggattg ttcaatgcat
     8821 gataggtttt atcccattct tttgctttgc cttttgtgct aacgacttca acacccttat
     8881 tgacaaaaat atttacaatg ttaaccgctt cgttaagtaa tccacccggg tttccgcgta
     8941 aatcaaaaat caacccttta agttttgtct ttgcttccaa agcttgcaat gcatttttta
     9001 cttcctgtcc tgcattttct gtaaatccgg taaggcggat ataacctaaa tcgttatcaa
     9061 taattccact gtaagaaaca ctgtttattt taatctcttc acgtactaca tttttatcaa
     9121 tcaggtttgg ttcaccttca cgtctgatag ttattttaac tgaagttttt ggctgacctt
     9181 taagcagctt actcacttca tcattctttt ttcctttaat gggcttaccg tctagttcaa
     9241 caattacatc acctgcacgc aggtcggctt tttgagcagg aaatccttca taaggatcgg
     9301 taatgattac ttcgttattg cgcgaaccga taagtgagcc aatgccacca tactgtcctg
     9361 tagtcatgaa acgatagtct tcaatttcac tttcgggaat ataattggtg tatggatcaa
     9421 gtgaggcaag catatcatca atgccttttt tcatcaggtc accgggattt gtttcatcaa
     9481 cataataaat gttcaactca cgaaacaagg tagcgaaaat gtcgaggttg cgcgatattt
     9541 caaaataatc gtcactgaaa ctataaacag aaaaagcaga tatgccaacc agaataacgg
     9601 caacatacaa tctgattctt ttacgcattt tttgtagcat cttataaact atttaatcaa
     9661 ttaatatttt taaaaatcat taaggcaccg gatgatgtcc atggccacca aatggattgc
     9721 aggataatat tctttttagt gataaaaaac cacctttcaa cggtccatac tttttcagcg
     9781 cttccataca aaactcgctg catgtaggat aataacgaca tgacataggc aacaatggcg
     9841 atatgaccat tttataaaac tttactatgg ctatcagaat cctgttcagc agtttgatca
     9901 ttcaattctt ttaaacgttg taaaattagg attattttag attgtatctc tgtgaaggta
     9961 gcgttaatat ttccattata aacaaacata atatgtatga cctgctcatt ttttttcagt
    10021 tcgttgctca atatctgctt attaagtctg taactttcac gaataagcct tttaatacgg
    10081 ttgcgggaaa cggcacttct tatttttcgt ttcggtacac ttatggcaat tttaacagga
    10141 actgttgatg tgttttcttt taacttcagc caatgcactc tgaacggaaa acggttaaac
    10201 gctgcaccct gactaaaaat cagttcaaaa tctttccttt tacaaagcct ttctgcctta
    10261 gaaaatgtgt ttcgcaaagt gccctcattc attgttttgc aaaagtacaa acattgtatc
    10321 ggacagataa ttatttttca ggattatatt gtttttggtt ctaactttat aatttggcga
    10381 aaatttattt atgcttaaat ccgggctggc actatgttta ttgttcatac tatcgttggg
    10441 caacatcaat gctaaggaaa ctatttttaa agaacaacgc ataggttttg gatattatcc
    10501 gtcttttcat ggatctcaaa atttttggtt gaactacagt gtaaataagc gcagccgcaa
    10561 aaccatttat tcaatccgag gcatgtataa tttccgctcc gaaaaagcac aagaagtaag
    10621 taattatgct attggcccct gtgtttctgt aaagctacta tcgtttttta aggaaagatt
    10681 agaaattcgt cccacactgt atatccctta tgtaaatttt aaatacgaaa aatcaaccta
    10741 ccctggaagt ttatatgcaa cacagtataa tggtgcagcc ttattgcccg gtttaaatat
    10801 tcagaccgaa cttaccgaca gaattggtgt gcgctatggt gctaatgcag gaatctatta
    10861 tgctaaaaca cgtgaaccca tttttgaaca gaaaaacgga gtaacaactt atgatgttta
    10921 caccgataaa aatatgtatt ggataatgct gattgatttt agtattacaa ttaaaatcac
    10981 aaaaaactat ttaccgcatc agcgataaaa aacaatcatt attctcgcca ttcctgacct
    11041 gtaagattca gaaaaacgtc ttcaaggttt gcttttttaa cttctttttt tcgctcaaaa
    11101 ccttttgcta ctaaatcgtc tatcaactgg tcaggagtat cgagtgcaat aatttttcca
    11161 ttttcgataa tagctactct gctgcacaac tcttctgcct cgtccatata gtgtgtagta
    11221 ataactacag tagtgccttc attttttatc tgtcgaatca agtcccaaag atttcttctt
    11281 gcctgtgggt ctaatcctgt agtaggttca tccagaaaaa tcactgcagg tttgttgatt
    11341 aatgttgttg caatagaaaa tcgctgtttc tgcccacctg acaattcttt gtattttgat
    11401 tttgccttat ccgttaagcc tactttctga agtaattcca ttgcattaac atcctgactg
    11461 tacaatccgg caaataggtc aatcaactca agtaatgaaa gacttggata atagcctgca
    11521 gcctgcaact gtacgccaat tatttttttt atcgcatcat catcagtacc aactttattt
    11581 cctttaatag ttatgtcacc tgatgttttg gcacgaaggg tttccattat ttcaagcgtt
    11641 gtcgttttac cggcaccatt aggtccgagt aaaccaaaaa tctcaccttc atacacttca
    11701 aaagatattc ctttaacagc gtgaaagcta tcgtaatgtt tgactaaatt atttaccgtt
    11761 ataattggat tcatgatgtg caaagttata acatttgact tccttcctca aaatgccatg
    11821 aagttttacc tttgccaaaa ataaaccggt gttaaaagtt gacgacatac tggtatcgga
    11881 ccaggttatt acagagaaat ttatttgcga tctttctaaa tgtaaaggag catgttgtgt
    11941 agaaggtgaa tcgggcgcgc cattagaaga agatgagttg aaagcattgg aaaaagttta
    12001 tcccaaagtt aaaccttatt taaccgatga gggaataaaa gctattgaag cgcagggatt
    12061 tcatctgaaa gatgatgatg atgaatgggt gacacctttg atgtggcccg gtggcccatg
    12121 cgcttacacc gtttttgaaa atggtattgc actatgcgga attgaaaaag catatcgaga
    12181 taaaaaaata aaattttata agccaatcag ttgtcatctt tatcctatac gtgttttaaa
    12241 aacaaaatcg ggtgatgcat taaactatca tgaatgggaa gtttgcagtc ctgcctgtaa
    12301 gttgggaaag aaagaaaaag ttcgggttta tgaatttctg aaaactgcac tgaccagaaa
    12361 atatggcgaa gcatggtaca acaaattaat aaaggctgca caggagtatt tacagacact
    12421 gacaaaaaaa taatatcttg acaaaacaat taaatctttt acctttgttt aaccataacc
    12481 gaatcaaatg tatttaccta agcatgtaat cagttggttg ttttttttaa ttatcataca
    12541 atcagcaacc gcacaaacta caatttcagg aacagttacc gatcagaaaa ataatcctgt
    12601 tgcaggagcc aacatcgtta tccgcgacag ttatgatggt gccagcagcg atgcttcggg
    12661 gaaatatcaa ttcaatgcaa atgattccgg tgatgtgata atagtttgca gcctgattgg
    12721 ttttagtcct tcagataaaa aaataaaact acaaggtaac acacttgaaa taaatttcaa
    12781 actcaaagaa acaagcaatg agctgaatac agtaactata acagcaggag cctttgaggc
    12841 aagtgatgaa aagaaaatgg tcattttacg accattggat attgttacaa cagcaggttc
    12901 gcagggtgac atttacggag cgctgcaaac acttcccggc acacagccta cacaagatca
    12961 ggaaggactt tttgtgcgtg gtggcgatgc agcagaagcc aaaacactta ttgatggaat
    13021 agaggttgca aacccatatt tctcaagtgt tcctgatgtt ccgcaacgca gcaggttttc
    13081 tccttttatg tttaaaggca caaacttcag caccggaggt tattctgcgc aatatggtca
    13141 ggccatgtca tcggtgcttg cattagaaag cgaagacctt cctccacgct ctgtcggaaa
    13201 cattggcatc atgtctgttg gtggtggtgg tggttacatg catcgttgga aaaaaacttc
    13261 agcaggtgca tttgccaatt attttaacct tgcaccctat tttgctatca tcaaacaaaa
    13321 ccgcgactgg gattctcctc cacaaacttt cagcagttct gcagtgttca gacataagac
    13381 atctgaaaca ggaattatta aagcatttat caattatcag tacagcgacc tctcgctcaa
    13441 cttcgacaat ctggaagacg ttaccggaaa aagtaaaaat cattttggtc tcaacaacta
    13501 caacatcttt tctacgcttt catataaaga agtgctcaat aaatggacag tgcaggcagc
    13561 aggatcttac agcaaggata ttaatcgtat ttatgcaagg ccacaacatc tgcttttcag
    13621 taatcaactg tggcaagggc gtgcagtagt gtcccgttca ataagtgaat tgagctccat
    13681 acgtggtggc gtagaagtaa aatataatga cttcagtaac ctctataata atttcagagt
    13741 ggatagtaca gaaatgttta cggcagtttt tcttgaaggc gatgcctaca tcacaagaaa
    13801 attagtagct cgtgccggac taagagttga aaattcatac ctgcttcgtg caagtaatct
    13861 tgcaccacgc atcagcgtgg cttataaatt aggaaagttt gatcagttga atattgccta
    13921 tggagattat tatcaaacac ccgataaaaa ttatctcatc tttaccaacc tcgatcattt
    13981 tgaaaaagca acacattaca ttttgaacta tcagtatgta aatgaaaaaa gaacctttcg
    14041 tgttgaagca tattataaaa aatacaacca cttagtgcag actacaaata tggcatcgga
    14101 tgtatatgct acagcatttg ctaccggtga tgatggaaaa ggctatgcac aaggaataga
    14161 ttttttctgg cgcgataaac aaacttttaa atatgtggat tattggattt cctattcatt
    14221 cctcgacacc aaaagaaaat atttatacta tccgtttgaa gtgcagccca cctttgcttc
    14281 aaagcatgtg gtgagtgtag tgatgaaacg ctggtttcca aaattgcgaa gcagcattgg
    14341 tttcacctat gtgtatgcag caggaagacc ttattataat cctaataaaa ccaatgaaaa
    14401 taattttctt tccgatcgca ctaacgatta taacaacctg agtttaacct ggagctattt
    14461 aacatccatt ggcagacact ttacagtaat ttctgctgcc gtaaataatg tgttgggtat
    14521 tgaaagtgca tacagctatc gttacagcac tgacgggcta agaagaagtg ctgttggccc
    14581 gacttccaaa cgctcctttt ttattggtgt atttatcagt ttcggagaag acagaacaga
    14641 ataaaaaaat tcaaatcaaa ttataaaatg aaaaaactca cattcctttt actctttatt
    14701 gcaggcaaca tgtttgcaca aagcaacagc aagtttgaaa aaactatggt taaacaaatt
    14761 gatttgcttg ataaagccaa agcaggtact gactttgtga atgccgccaa tgcatttgag
    14821 cgcattgcaa atgcagaaac caaggagtgg ctgcctttat actatcaggc ttatgcttta
    14881 gtactttctt caaatgatga aaaagacaat gtaaagaaag atgccgtttt agacaaagcg
    14941 cttgaaattg ccaataaagc agataaactc tcacctgata attcagagat tgttacactg
    15001 caatcgtgga taacctcaat gaaaattggt attgaccctg caacacgcgg acaaaaatta
    15061 gggatggaag caggcatgct tacagcacat gccattgaac tgaatccgga aaatccacgt
    15121 ccctattttc tgcaaggtac aggattgttg tttactcctg aacaatttgg tggtggcaaa
    15181 gccaaagcat tacctgttct tgaaaaggca atagagaaat ttaatgcatt taaacctgcc
    15241 aacgctatga tgccaaactg gggaaaagag tttgctcaga aaatgcttga tgagtgtaag
    15301 aaataggctt tagtttggaa gggttggaaa gtttggaagg ttggaaagtt tggaaggttg
    15361 gagggttgga aaagttgtaa tgtttaaagc agagaatgcg cgagcgaaac gctcgggcat
    15421 tccttgctta atgcacctta aactaaaaaa ggattgtaca ttttttcaaa acctattgtt
    15481 gtttcggggc catgacccgg aagcactttt acatctgcag gcagtgtaaa cagttttgtt
    15541 ctgatgctat tcaacaatgt ttgctcatca ccaccgggta aatcataacg gccgatgctc
    15601 atctgaaaca atacatcacc tccaataata attttctctt gtttatcata aaaacataaa
    15661 cttccggggc tgtgaccggg agttaataat atttctaatg aaatattacc aaatgaaatg
    15721 atgtctcctt cttccaaata cttttctgcc gttggtgatg gctccagaaa tattccccat
    15781 tgcttactgt attctcctgc tgcttcaagc aatcttaact ctaactgatg aaactgtggt
    15841 tttaatccga aagtatcata gataagtttg tttccgaaaa tatgatctac atggcaatgt
    15901 gtatttagca acaaaacagg gtttagtttt tgttctgcaa taaaactgag taattcttcc
    15961 ttctcttcag gcatatagca tcccggatct ataatggcac actgtgctgt ttcatcataa
    16021 acaatatatg tgttttcctc aaaaggatta aatgtaaatt tctttatctg catgttatta
    16081 gccaatgaaa gtgcaaatta aagaatatta acgtgagttt agaaggttta gactgtttgg
    16141 aaagttttga atgtttagaa tgttattaat gcagctaatt tttaagtttc tgatttgtga
    16201 cttcttgaaa agtttgaagg tttagaatgt tttgaaagtt acaatgtttt gaaagttaca
    16261 atgttttgaa agttattgat ttcgctaatt tttacatttc tgatttgtga cttcttgaaa
    16321 gtttgaaggt ttggaaagtt tagaatgtta caatgttttg aaagttattg atttcgttat
    16381 tttttaagtt tctgatttgt gacttcttga aagcttgaaa gtttgaaggt ttggaaagtt
    16441 tagaatgtta caatgttttg aaagttactg atgcagctaa tttttaagtt tctgatttgt
    16501 gacttcttga aagtttgaag gtttaaaatg tttggaaagt tggaatgcag gattttaatt
    16561 ggaattttac ttagatgatt tgttgattag atacttagat aatgttaaat gtttgttgta
    16621 ataaagatta ttttatcaag tgtaatacat tagcctaaat tttaaatatg gaatacatca
    16681 aacatctttc taaagataaa aaattagcat cggtaattaa gcaacatgga actgtagaac
    16741 ttaaaaaaag aaaagacttg tttctgagtt tatgcggttc tattatatca caacagttgt
    16801 ctgtaaaagt tgctgacgta attaaacagc gtttttatga tttatttgat ggaaagccta
    16861 ctcccgaaaa aatacttcag gcaacaacag aaactttaag aagtattgga ttgagtaatt
    16921 ctaaagcaag ctatattaaa aacattgcag cttttgcact ggaaaacagt ttgcagttga
    16981 acaaggtaaa taaaatgagt aatgacgaat ttattgaata cattactcaa ataaaaggtg
    17041 ttggccgttg gactgcagaa atgattatga tgtttaccct aggtcgcgaa gatgtttttt
    17101 ctgttgacga ttatggtatt cagcaatccg ttatcggact ttataagatt aaaatatccg
    17161 ataagaaaaa gttgcgcctg cgaattttag ctatcgctga gcaatgggca ccttacagaa
    17221 cttatgcatg catgtatctg tggcgatggc gagataatgg ttgaaaatca aatcttattt
    17281 gctcataact tcatggatac cacatataaa ctaatataat tttctacatt tttgcgttat
    17341 tgctttgtat tcatgaaaag atatttattc attcttataa cattgttttc atcagttgct
    17401 ttagcacaac aatccggcaa cggttttgct gtggtagagc tttttacctc acagggttgt
    17461 ggcagttgtc cggcagcaga aacagttctg aaaaaagaaa tggatgatgc cattaagaat
    17521 aataagcctg
//
LOCUS       DAORQT010000013        17473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_272_length_17473_cov_11.093122, whole genome shotgun sequence.
ACCESSION   DAORQT010000013 DAORQT010000000
VERSION     DAORQT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17473
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_17473_cov_11.093122"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PK134_01075"
     CDS             complement(<1..1259)
                     /locus_tag="PK134_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB37477.1"
                     /translation="MGSIFIGLYRLFVRKPILFAGFVLLVAAVSIAGIKRLNITENIF
                     AILPKGQEFQKFNSLLESKNISDQVVFSLSLTKNSNPDEYADVAEHVKSTIDSASANL
                     LSDVVAVRPDVQQEVYDYYFSHFPLLIDSNYYSLIERKIQDDSIQFSIKNNYERLTNP
                     SGLFIKQFILNDPLSIPAEYFRQLGITGNSSNYVVEDGIIFTPGKKSILITGRLNYDS
                     RDAEKNIQLSDQLAAIEKKWNAENSEYKLSHFGTFEIAAQNAVQIKKDTNLTMILSLS
                     LIMLILILYYRKLSIPILFILPAVFGGMLALGLMGFLRPEISAISLATGAIVIGIILD
                     YSFHFFTHLQHVKSIEETLKEISVPLLTGSVTTVLALLALNYTNSVVLGDFGLFAAMA
                     LAGSAFFTLAGLPVIITLTRFDYTQHKT"
     gene            1394..2488
                     /gene="hppD"
                     /locus_tag="PK134_01080"
     CDS             1394..2488
                     /gene="hppD"
                     /locus_tag="PK134_01080"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRB37478.1"
                     /translation="MAEDFLPLNGTDHVELYVGNAKQSAYYYQMAWGYELIAYAGPET
                     GIRDRASYVLQQGKIRLVLTSAMHPDSEITRHVAQHGDGVKFLAIWVDDAEKSFYETM
                     SRGAKAHTQPTVLSDKFGEVKISAIHTYGETVHKFVERKNYNGAFLPGYSPAKSSVKT
                     TPVGLKHVDHCVGNVELGRMNEWVKFYEDVMGFKLLITFDDSDISTEYSALMSKVVSN
                     GNGYIKFPINEPADGKRKSQIEEYIEFYHGAGVQHVAIATDDIISTVTELRNRGVEFL
                     HVPEVYYEDVWDRVGKINEDVATIKKLNILVDRDDEGYLLQLFTKPVQDRPTVFFEII
                     QRCGAKSFGKGNFKALFEAIEREQALRGNL"
     gene            complement(2512..3132)
                     /gene="recR"
                     /locus_tag="PK134_01085"
     CDS             complement(2512..3132)
                     /gene="recR"
                     /locus_tag="PK134_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRB37479.1"
                     /translation="MEQYPSRLLEEAVNELSRLPGIGRKGALRLALHLLKQNPADVER
                     LGNAVIQLRSSVVYCHQCHNISENTLCAICANPRRDGSVICVVQDIRDVMAIENTAQF
                     NGMYHVLGGIISPMDGIGPSQLNIDSLVEKTANGNINEIIMALSATMEGDTTVFYISK
                     RLKDIDVKISILARGVAIGGELEFADEVTLGRSLINRVAYENTLVK"
     gene            3244..4707
                     /locus_tag="PK134_01090"
     CDS             3244..4707
                     /locus_tag="PK134_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRB37480.1"
                     /translation="MSPVLILSVVLVYTILLFAITFYTSRNADNQSYFVGNRSSRWYV
                     VAYGMIGASLSGVTFMSVPGDVGTGNFSYFQMVLGYLLGYAVIAFVLLPVYYRMNLTS
                     IYTYLKSRLGEKTYKTGAFFFILSRVVGASFRMYIVVYVLQLFIFQAWGVPFWVTVFV
                     FVLLILSYTFKGGVKTIVWTDSLQTTFMLLSLFISVYLIAKELNFSFADVVSSIKSSP
                     YSDIFVWDWRSKVFFPKHFFGGMFIAIAMTGLDQEMMQKNISCRNVRESQKNMMTFSI
                     ILVFVNFIFLCLGALLYIFIQHQGIVIPERTTDDLFPTIAMHYLGTFSAICFIIGLIS
                     AAYPSADGALTALTASVCIDFLGFEEGKYTEAQKTKIRKSVHVIFGFVLLGVIVLFHA
                     INDQAVIRQIFRAANYTYGPLLGLFAFGILTKRNVIDKSVPVIGILSPVICYIIDVNS
                     VQWLGGYKFGNELIILNGGITFMGLLLLTKHKTAAQE"
     gene            4707..6137
                     /locus_tag="PK134_01095"
     CDS             4707..6137
                     /locus_tag="PK134_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRB37481.1"
                     /translation="MENIQQKNSSTSYNVVTNEILEKLKAICGEKYVVNDDESCATYG
                     SDETEDLFFKPEVIVKPSDANEISAILKLANSHFIPVTPRGAGTGLSGGALPVHGGII
                     LSTERLNKIIDIDEKNLQCTVETGVINQHLRDAVEAKGLFYPPDPASRGSCFIGGNLA
                     ECSGGPKAVKYGVTKDYVLNCEVVLPTGEIIWTGANVLKNSTGYNLTQLMIGSEGTLG
                     VITKVVLKLIPLPTETLLMLIPFRSAEKACEAVSAVFRAGITPSAMEFMERDAIEFVM
                     KFTDVKVPLKEDHVAHLLIEVDGNNKDVLFKDCEKISEVMYQHDCDEVMFAESAQQKA
                     DLWRARRAVGEAVKSHSVYKEEDTVVPRASLPVLLAGVKKIGSKYGFRSVCYGHAGDG
                     NLHVNIIKDGMTDEEWNRKLPLGIQEIFELCVSLNGTISGEHGIGWVQKNYMSIRFSD
                     VEIALQKGIKKLFDPNNILNPSKIFA"
     gene            complement(6138..8453)
                     /locus_tag="PK134_01100"
     CDS             complement(6138..8453)
                     /locus_tag="PK134_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRB37482.1"
                     /translation="MNNYLDELNPSQRDAVECMKGPSMIIAGAGSGKTRVLTYRIIHM
                     INNQVDPFNILSLTFTNKAAREMKKRIMEKVGHDAQNLWMGTFHSVFARILRIEAEKI
                     GFPTNFTIYDSDDSKSLIKKIINEQNLDDKTYKPSLVLNRISSAKNNLFSVSDYLNHS
                     ELTNEDKMAGKPKIGMLYEIYQKRLKQSDAMDFDDLLFNTYLLLKNHPEALSKYQNKF
                     KYVMVDEFQDTNYAQYIIVRKLAAAHENICVVGDDAQSIYAFRGANIQNILNFEKDYP
                     DLKTFKLEQNYRSTKTIVGAANSVIANNRNQLKKEVWTENTGGEKIRLLRALSDNEEG
                     AQVAQAIFEDKMNLHFKNKEIAILYRTNAQSRSLEEALRKLNIPYRIFGGLSFYKRKE
                     IKDLLAYFRLTINPYDEEALDRIYNYPTRGIGKTTYEKLLVLADARTCTLWDVMQDIN
                     DREIDIATGTKTKINEFVTMMQSFSVLAKTQNAYDVASHIAQSSGLLKELFNDKTAEG
                     VSQYEHIQELLAGIKEFSDGAQTALEAPSAELEQSETKLLNEYMQDIALLTDAETDDG
                     NDDKVSLMTIHQAKGLEFKEVFVSGLEENLFPSQLAVNSRDELEEERRLFYVAITRAE
                     KRLTLSYAMTRYRWGTLTGCEPSRFLEEINPEFLDALPVSDTKMSPTAIRQGNGVPSF
                     SPNSFTKNNLKPVFKKPVAKQTDPKLFENFIPDDPKRIEIGMSVLHQRFGKGKVMQLE
                     GNLPDLKATVNFEGEGQKQLLLKYARLKIVE"
     gene            complement(8569..8952)
                     /locus_tag="PK134_01105"
     CDS             complement(8569..8952)
                     /locus_tag="PK134_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37483.1"
                     /translation="MITKIEMNHVELLEWLKALDFYKNELRFWRKHLSHVVVSNTNKD
                     ILREAEHFQNQVIIQVDQIDLLHHDVKQFENKLDEEFESVSKGGKFSQQIVDVEASLR
                     ERMKTFEHIYVDLKHDYYSFLRKYM"
     gene            9108..9590
                     /locus_tag="PK134_01110"
     CDS             9108..9590
                     /locus_tag="PK134_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRB37484.1"
                     /translation="MGSLLAFAWTLVGYILGNETQEKLKWLSMLVMIIIVVFCFRAVI
                     NEEKKANEGFISFGKAFGMAFKTGLVMALCSAVFTYLYFNFINPEYMEFAIQKAQEQM
                     QSQGLSDEQIDMSMKMQAKFMTPAMMTIVAFFSSVIIYVIVSLIMGAIMKKENPNEMS
                     "
     gene            complement(9587..10819)
                     /gene="rocD"
                     /locus_tag="PK134_01115"
     CDS             complement(9587..10819)
                     /gene="rocD"
                     /locus_tag="PK134_01115"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRB37485.1"
                     /translation="MEMSKETTVGSSYFIELEERYGAHNYHPLPVVLSRGEGIFVWDT
                     EGKQYFDFLSSYSAVNQGHCHPRIVASLVEQAGKLALTSRAFYNDKLGEYEKYATELF
                     GYDKLLPMNSGAEAVETAIKLCRKWAYKNKDIARNQAKIIFCEGNFHGRTISIISAST
                     DPTASTNYGPFTPGYVIIPYNNLAALEDALSDPNVAGFIVEPIQGEAGVVVPDDGYLS
                     KAAALCRQNNVLFIADEIQTGLARTGKMLACDYENVKPDILILGKALSGGMLPVSAVL
                     ANDNIMLAIQPGEHGSTYGGNPLACVVATTALQVILDEKLAENATKLGKILRDELNKF
                     KHEMLTLVRGKGLMNAIVIKPSKGKDAWDVCLKLAANGILAKPTHGDIIRFTPPLVMT
                     EEQLYECIAIIKKTLSEF"
     gene            10978..11961
                     /locus_tag="PK134_01120"
     CDS             10978..11961
                     /locus_tag="PK134_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRB37486.1"
                     /translation="MKRKTFSIALLFCIISLLVYGWGFFGHKRINRMAVFTLPAQLMR
                     FYKANIDFIEEHAVDPDKRRYAVKDEAARHYIDLDRYGESPLDSLPKNWKAAVEKYSK
                     DTLMAHGIVPWHINVMLGRLTDAFRNKDYNKILRYSADLGHYVADAHVPLHCTQNYNG
                     QLTNQVGIHGLWESRLPELFADDYDYFIGKAQVIEKPMDYIWEIISQSFAAKDSVLLF
                     EKELNARFDADRKYAFEQRGTTMMRVYSVDYSKQYQDMLNGMVERRMRQAIIAVGSFW
                     LTAWTNAGQPNLDGIEKITISEEMKREIENEDKMWRTGKVLSPGGHADDDH"
     gene            complement(12055..12585)
                     /locus_tag="PK134_01125"
     CDS             complement(12055..12585)
                     /locus_tag="PK134_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB37487.1"
                     /translation="MCEKYKIFPDGLYFVILTTVGWIDVFTRAIYCDEIVKNLNYSID
                     NKKLKVFAFCIMPSHIHLIAKTEDDVSLSDMLRDFKSYTKQIIKMIEESATESRREWL
                     LYMFEFYGNKNAHNVKYQFWEQHNHAFDLFSNKFIDQKVNYIHNNPVEARIVTEPHFY
                     VYSSANQFTDLKLAEL"
     gene            complement(12700..14950)
                     /locus_tag="PK134_01130"
                     /pseudo
     CDS             complement(12700..14950)
                     /locus_tag="PK134_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            15387..16007
                     /locus_tag="PK134_01135"
     CDS             15387..16007
                     /locus_tag="PK134_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37488.1"
                     /translation="MIMKKRISLALLILIAGVVNGQSNNPAPNGFANFNEFSHNTPSM
                     TLNFSVKQRTQGNVFMTGGITNYRLENIQPKSDKEKLEKEVWGVMVHDTVYINSYPYS
                     KVKGYNQIIDKGYYTYFIGEPARTEKEQRDLGIIKPTESQIAVCCQAGYVISPDGTIK
                     LLRPELLSELCKDNEEISKEIAAAKLKLEDVHQMFACLKKYNATKK"
     gene            16078..17280
                     /locus_tag="PK134_01140"
     CDS             16078..17280
                     /locus_tag="PK134_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRB37489.1"
                     /translation="MPRVNDMNNKNILISGAGIAGTTLAFWLKKYGFNPTIVEIALKL
                     REGGYAIDFMGAGYDVALKMGIADELKKVDIDFSKLKLVDKNDQEKGSMNYEKVKNLM
                     NGRAMTLLRSDLAKTIYNSLNNEVEIIFDDTIQQIEQNTGEVKVSFKNGNKRNFDLVI
                     GADGLHSKVRNLVFGDENQFEKYLGYYTSSYTINNFSIGNNAFSMYNYPYKQVAIYSN
                     SRNQTTAFFIFSSAEQLTYSQRDIEKQKQIMRQEFENGGWKCHQLLSALDTTTDFYFD
                     SVSQIEMDKWSNGRVALVGDAGYCPSLLSGKGSTLAMVGAYILAGELKEANGNYQQAF
                     EEYEKQFKSFMEKKQKSAQSFAKSFVPKSSFGIWLRNLVFKLMSISVFSKLFINQFKD
                     IGLKLKNY"
BASE COUNT         5466 a         3298 c         3255 g         5454 t
ORIGIN      
        1 gtcttatgct gtgtgtaatc aaagcgggtc aaagtaatta ttacaggtag gcctgcaaga
       61 gtaaaaaaag ccgaaccagc caaagccatt gctgcaaaca agccaaaatc acccaataca
      121 acagagttgg tataatttaa tgcaagcaaa gccagcactg ttgtcacact tccggtaagc
      181 aacggtacag aaatttcttt taaagtttct tcaatagatt ttacatgttg tagatgagtg
      241 aagaaatgaa aggaatagtc caggatgatt ccaattacga tggcgcctgt tgctaatgat
      301 atggctgaaa tttcagggcg aagaaaaccc atcagtccaa gtgctaacat gcctccaaac
      361 actgcaggta gaataaataa tattggaata cttagttttc tgtaatacaa aatcagaatg
      421 agcatgatta gtgataatga aagaatcatg gtcaggttgg tgtccttctt tatctgtact
      481 gcattttgtg ctgcaatttc aaaggtgccg aaatgtgata atttgtattc agagttttca
      541 gcattccatt ttttctctat agcagcaagt tggtcactaa gttggatgtt tttttcagca
      601 tcacgactat catagttcag acggcctgta ataagaatag actttttgcc cggtgtaaaa
      661 atgataccat cttccacaac ataattgcta ctatttcctg taatgccaag ttgtctaaaa
      721 tattctgcag ggatgcttaa tggatcgtta agaataaatt gtttgataaa taaaccggat
      781 ggattggtaa gtctttcgta attgttttta atcgaaaatt gtatagaatc atcctgtatt
      841 tttctttcaa tcaaagaata ataattacta tcaatcagca gagggaagtg cgagaaataa
      901 tagtcgtaaa cttcttgttg cacatcaggt cttactgcaa caacatcact caataaatta
      961 gctgaagccg aatctatagt actttttaca tgttcggcca catcggcata ttcatcaggg
     1021 tttgagtttt tcgttaatga taaagaaaac acaacctgat cagagatgtt ttttgattca
     1081 agaagagaat tgaatttctg aaactcctga ccttttggaa gaatagcaaa tatattttca
     1141 gtaatgttta gtcttttaat tcccgcaata ctaacagcag caaccagcaa aacaaagcct
     1201 gcaaacagta taggctttct tacaaacaga cgatataatc caataaaaat acttcccaag
     1261 aagaaccgat tttacaacgt aactttcagt gtgcgaattt ataggaatta atgtgtcaaa
     1321 ctttcataaa acgtttatat actttcattt ttaaaatgat tacttttgcc tctcattcta
     1381 atattaaatc aaaatggctg aagattttct tccgttaaac ggaaccgacc acgtagaact
     1441 ttatgtaggc aatgccaagc aatctgcata ttattatcag atggcatggg gctatgaact
     1501 tatagcttat gccggcccag aaaccggtat tcgtgacaga gcatcgtatg tattgcaaca
     1561 aggcaaaatc cgtttggtgc tgaccagtgc catgcatcct gacagtgaga ttacacgaca
     1621 cgtagcgcag catggtgatg gtgttaagtt tttagctatt tgggttgacg atgctgaaaa
     1681 gtctttttat gaaactatga gtcgtggtgc aaaggcgcac acacagccta cggttttgag
     1741 tgacaaattt ggtgaagtga aaatttctgc tattcatact tatggcgaaa ctgtacataa
     1801 gttcgtagaa cgaaaaaatt ataatggggc ctttttgccc ggatattcac cggccaaaag
     1861 cagtgtaaaa actactcctg tcgggttaaa gcatgttgat cattgcgtgg gcaatgttga
     1921 gttggggaga atgaatgaat gggttaagtt ttatgaagat gtcatgggat ttaagttgct
     1981 gattaccttt gatgacagcg atatttctac agaatattct gctttaatga gtaaggttgt
     2041 cagcaatgga aacggttaca ttaaattccc gattaacgaa cctgctgacg gtaaacgcaa
     2101 atctcaaatt gaagaataca ttgaatttta tcatggtgcc ggagtgcagc acgttgccat
     2161 agccactgat gatataattt caacggtaac tgagttgcgc aacaggggag tagagttctt
     2221 gcatgttccg gaagtttact atgaagatgt ttgggatcgt gttggaaaaa ttaacgaaga
     2281 tgtagctaca attaaaaaac tgaatatttt agttgatcgt gatgatgagg gctacttgct
     2341 tcagttgttc accaagcctg ttcaggacag accaacagta ttttttgaaa tcattcagcg
     2401 ttgcggtgct aaatctttcg gaaaaggaaa cttcaaagcg ctttttgaag ctatagagcg
     2461 cgagcaagca ctgagaggaa acttgtaatg acgtactgcc tatgatggca gttattttac
     2521 caaagtgttt tcgtaggcta cacggttaat taacgaacgg ccgagtgtaa cttcatcggc
     2581 aaattccaat tcgccaccaa tggctacacc tcgtgcaagt attgaaattt taacatcaat
     2641 gtcctttaat ctttttgaga tataaaacac agttgtgtca ccttccatgg tagcactgag
     2701 tgccattatt atttcgttta tatttccatt agcagttttt tcaaccaacg aatcaatatt
     2761 cagttgcgaa gggccaatgc catccatggg agaaataatg ccgccaagaa catgatacat
     2821 gccattaaat tgtgctgtgt tttcaattgc cattacatca cgaatatcct gtacaacaca
     2881 aattacactg ccgtcacgtc ttggatttgc acagattgca cataaagtgt tttcagaaat
     2941 attatgacat tgatggcagt aaactaccga tgaacgtaat tgtattacag cattgcccaa
     3001 acgctccaca tcagcaggat tttgtttgag caaatgcaat gcaaggcgca atgcaccttt
     3061 acggccaatt cccggcagac gcgataattc gttaactgct tcttctaata aacgtgacgg
     3121 atactgctcc acctaatgaa aatttaaatg atgttgtcaa atatacgttg tttaaaaata
     3181 ccatctttca tgcgctgtca aaggttttca aacagttatt cttgtattct aaatttcaaa
     3241 caattgagtc ctgtcttaat tttatcagtg gttttggttt ataccattct gctgtttgca
     3301 attacatttt acacttcgcg caatgccgac aatcagagtt attttgttgg taacagaagt
     3361 tcgcgttggt atgtggttgc atatggaatg atcggggcat cgttatcagg tgttacattt
     3421 atgtcagtac ccggtgatgt tggtacagga aatttctctt actttcaaat ggtgcttggc
     3481 tatttgttag gctatgctgt cattgctttt gtgttgctac ctgtttatta ccgtatgaat
     3541 ctgacttcaa tctatactta tctgaagtcg aggcttggag aaaaaacgta taagacaggt
     3601 gcattcttct ttattctttc acgtgtagtt ggtgcttcgt tcagaatgta tattgtggta
     3661 tatgtgttgc agctttttat ttttcaggct tggggcgttc cgttttgggt caccgtattt
     3721 gtgtttgtcc tgctgatact ctcatacaca ttcaaaggtg gtgtaaaaac aattgtgtgg
     3781 acagattcat tgcagacaac ttttatgctg ctttcgctct ttatttcggt ttatcttatt
     3841 gccaaagagc ttaattttag ttttgctgat gtagtatcat caatcaaatc aagcccatac
     3901 tcagatattt ttgtgtggga ctggaggtcg aaagtgtttt ttccgaaaca tttttttgga
     3961 ggaatgttta ttgccattgc catgacgggg cttgatcagg aaatgatgca gaaaaatatc
     4021 agttgcagaa atgtacgcga atcgcaaaaa aatatgatga cattcagcat cattttagtt
     4081 tttgtaaatt ttatttttct ctgtcttgga gcattgttgt atatttttat tcagcatcag
     4141 ggcattgtta ttcctgagcg cacaacagat gatttgttcc cgacaattgc catgcattat
     4201 ctcggcactt tttcagctat ttgttttatc attggattga tatctgctgc ttatccaagt
     4261 gcagatggag cattaacagc actaacagca tcggtgtgta ttgattttct tggctttgaa
     4321 gaaggtaaat atacagaggc gcaaaaaaca aaaatcagaa aaagtgtaca tgtcattttt
     4381 ggttttgtat tacttggtgt tatcgttttg tttcatgcca tcaacgatca ggctgtgata
     4441 cgtcagattt tcagagcagc aaattatacc tatggtccct tgcttggact ttttgcattc
     4501 ggaattctaa ctaaaagaaa tgtaattgat aaatcagtgc ctgtaattgg gatattatcg
     4561 cccgtaattt gttatatcat tgatgtaaac tctgtgcagt ggcttggtgg atataaattc
     4621 gggaatgaat taataattct taatggcggc atcaccttta tgggcttact tttgttaacc
     4681 aaacataaaa ccgcagcaca agagtaatgg aaaatataca gcagaaaaat agtagcacat
     4741 catataatgt tgttacgaat gaaattcttg aaaaactaaa agctatttgt ggcgaaaaat
     4801 atgttgtcaa cgatgatgaa tcatgtgcaa cctatggcag tgacgaaact gaagatttgt
     4861 tttttaaacc ggaagtaata gtaaaaccgt ctgacgccaa tgagatttca gcaatattga
     4921 aacttgccaa ttcacatttt attccagtca caccacgtgg tgcaggcacc ggtttaagtg
     4981 gtggtgcctt gcctgtgcat ggcggaatta ttttgtccac agagcgacta aataaaatta
     5041 ttgacattga tgaaaagaac cttcaatgca cggtcgaaac aggtgtaatc aatcagcatt
     5101 tgcgcgatgc agtagaagcc aaaggcttat tttatcctcc tgaccctgcc agcagaggaa
     5161 gttgttttat tggcggtaat cttgccgagt gtagcggtgg acctaaagct gtaaagtatg
     5221 gtgttactaa agattatgta ctgaattgcg aagtagtgct tccaacggga gaaataatat
     5281 ggacaggtgc caatgtgctg aaaaattcca caggatataa tctgacacag ttgatgatag
     5341 gtagtgaagg tacattaggt gtcataacaa aagtggtgtt aaagttaatt cccttgccaa
     5401 cagaaacttt gctgatgctc attccttttc gatctgcaga aaaagcatgt gaagctgttt
     5461 cagcggtttt cagagcagga attacacctt ctgcaatgga gtttatggaa cgtgatgcca
     5521 ttgaatttgt gatgaaattt actgatgtaa aagtgccgct taaagaagat catgttgcgc
     5581 atttactgat tgaagttgac ggcaacaaca aagatgtttt atttaaagac tgcgaaaaaa
     5641 tttcagaagt catgtatcag cacgattgcg atgaagtgat gtttgcagaa agtgcacagc
     5701 aaaaagcaga cctctggcgt gcaagacgag ctgtaggcga agctgtaaag tcacattctg
     5761 tgtataagga ggaagatact gttgtgcctc gtgccagttt acctgtattg cttgcaggag
     5821 taaaaaaaat tggaagcaaa tatggatttc gcagtgtttg ctatggtcat gccggtgatg
     5881 gaaatctgca tgtaaacatt attaaagatg gcatgactga cgaggagtgg aatcgtaaat
     5941 taccattagg tattcaggaa atatttgaac tttgtgtatc gttgaatgga accatttccg
     6001 gagagcatgg aatagggtgg gtgcaaaaaa actatatgtc tatacgtttt tcagatgttg
     6061 aaattgcact tcaaaaagga attaaaaaac tctttgatcc caataatatt ttaaatcctt
     6121 caaaaatttt tgcctgatta ttctacaatt ttcagtcgtg catatttcaa cagcaattgc
     6181 ttttgccctt caccttcaaa gttcacggta gctttcaaat caggcaagtt gccttccaac
     6241 tgcatcactt ttcctttacc aaaccgttga tgtaatacac tcattccaat ttcaattctt
     6301 ttaggatcat caggaataaa gttttcaaac aatttggggt cggtttgttt ggcaacaggc
     6361 tttttaaata caggtttaag attgtttttt gtaaaactgt tggggctgaa acttggcaca
     6421 ccattgcctt gccttattgc agtaggtgac atctttgtat cactaacggg cagtgcatcc
     6481 aagaactcag gattaatttc ttcaagaaaa cgactaggct cgcaacctgt taatgttccc
     6541 caacgataac gtgtcatggc ataacttagt gtcagccgct tttctgctct ggtaatggca
     6601 acataaaaca aacgcctttc ttcttcaagt tcatcacgcg agttaacagc caactgcgat
     6661 gggaataaat tttcttcaag gccggaaaca aaaacttcct taaactcaag tcccttagcc
     6721 tgatgaatgg tcatcagtga aactttatca tcattaccat catcggtttc ggcatctgta
     6781 agcagggcaa tgtcctgcat atattcgttc agcaattttg tttcagattg ctccagttct
     6841 gctgatggtg cttcaagagc tgtttgtgca ccatcgctaa actcttttat acctgccagc
     6901 aactcctgta tatgttcgta ttgactgaca ccttctgcag ttttatcatt aaataattct
     6961 ttcaacaatc cgcttgattg agcaatgtga cttgccacat cataagcatt ttgagtcttt
     7021 gccaaaacag aaaaactttg catcatagta acaaactcat tgatttttgt ttttgttccc
     7081 gttgcaatat caatctcacg atcgtttata tcctgcatca catcccacag cgtacaagta
     7141 cgtgcatcag caagaactaa tagtttttca taagtggttt tgccgatacc tcgggtaggg
     7201 tagttgtaaa ttctgtcaag agcttcttcg tcatacggat taattgtaag tcgaaaataa
     7261 gcgagtaaat cttttatttc ttttcgttta taaaaagaca agcctccgaa aattctgtaa
     7321 ggaatattta gtttacgcaa tgcttcttct aaacttctag actgtgcatt ggtgcgataa
     7381 agaattgcaa tttctttgtt tttaaaatgc agattcattt tgtcttcaaa gattgcctgt
     7441 gctacctgtg caccttcctc attgtcgctt aatgccctga gtaatcttat tttttcgcca
     7501 cctgtatttt ctgtccagac ttctttttta agttgatttc tgttgttggc aattacactg
     7561 tttgctgcgc ctactatggt ctttgtgctt ctgtaattct gttctaattt aaaagtcttt
     7621 aaatcaggat agtctttctc gaagttgaga atattttgaa tgttagcacc tcggaatgca
     7681 taaatacttt gtgcatcatc acccacaaca caaatatttt catgtgctgc tgcaagtttt
     7741 ctgacaataa tgtattgtgc atagtttgtg tcctgaaact catcaaccat cacatactta
     7801 aatttattct gatacttcga caacgcttca gggtgatttt taaggagcaa ataggtattg
     7861 aataacaaat catcaaaatc catggcatca gactgtttca atcgcttttg ataaatttcg
     7921 tatagcatac ctatttttgg tttgcctgcc attttatctt cattggtcaa ctcggagtga
     7981 ttcagatagt cacttacaga aaacaaatta ttttttgcag acgaaattct gttcagtacc
     8041 aatgaaggct tgtaggtttt gtcatcaaga ttctgttcat tgataatttt tttaatcaga
     8101 ctttttgaat catcagaatc ataaatggtg aaatttgtag ggaatcctat tttctcggct
     8161 tcaattctta aaatacgtgc aaatactgag tgaaaggttc ccatccacaa attttgagca
     8221 tcatgaccaa ctttttccat gatgcgcttt ttcatttcac gggctgcttt attggtaaag
     8281 gttaaagaca aaatattaaa tgggtcaacc tgattgttga tcatgtgtat gattctgtag
     8341 gtaagcaccc tggtttttcc cgaacctgca ccggcaataa tcatactagg ccctttcatg
     8401 cactcaactg catcacgctg cgatggattt aattcatcta aataattatt cataatggaa
     8461 tttactactc ttcaaagata agggttgctt tggttattaa aatgaaaaca gggtatgttt
     8521 tttcataccc tgctattgaa aactttttaa ttaatttcaa actaatgatt acatatattt
     8581 tctgagaaaa gaataataat catgtttcag atcaacataa atatgctcaa atgttttcat
     8641 ccgttcgcga agtgatgctt ctacatcaac aatttgctgc gaaaattttc cgcctttact
     8701 tactgattca aattcttcgt ctaatttatt ttcaaattgt tttacatcat gatgcagcag
     8761 gtctatctgg tcaacctgaa tgataacctg attctgaaaa tgttcagctt cgcgaagtat
     8821 atctttgttt gtattgctta caactacatg tgacagatgt ttacgccaaa atctcaattc
     8881 attcttgtaa aaatccagtg ccttcaacca ttcgagtaat tcaacatggt tcatttcaat
     8941 ttttgttatc atacattatg ttcttttgta gctgctaaaa tagcacattt ccgacttacc
     9001 ttagttactg tctcggcaat aaatttcttt gtatgcttta aaaaactaat tttggcttgt
     9061 aaacattaaa tcaattcatg atgaaatcat ttatcaaata tggaattatg ggctcattgc
     9121 tcgcatttgc atggactttg gttggttaca ttttaggtaa cgaaacacag gaaaaactca
     9181 aatggcttag catgttggtg atgattataa ttgtcgtgtt ttgtttcagg gcagttatca
     9241 atgaagagaa aaaagctaat gaaggtttta tttcttttgg taaggctttt ggcatggcat
     9301 ttaaaactgg tttggtaatg gctttatgca gtgccgtttt cacttattta tattttaact
     9361 tcataaatcc ggaatacatg gagtttgcca ttcaaaaggc acaggagcaa atgcaatcac
     9421 aaggtttgtc tgacgagcag attgatatgt caatgaaaat gcaggctaaa tttatgacac
     9481 cggcaatgat gacaatagtt gcatttttca gctcggtaat tatttatgta attgtatctt
     9541 tgataatggg tgcaataatg aaaaaagaga atcctaatga gatgagttaa aattcactca
     9601 acgttttctt aataattgca atacattcat aaagctgttc ttctgtcatc accaatggtg
     9661 gtgtaaatct gataatatct ccatgagtgg gcttggccaa aatgccattt gctgctaatt
     9721 tcaaacaaac atcccatgca tcttttcctt tgctaggttt tatcacaata gcattcatca
     9781 gccctttgcc gcgaaccaat gtcagcattt catgcttaaa tttatttaac tcatcacgca
     9841 gaattttacc caatttagtt gcattctctg caagtttttc atcaagtatt acctgtaagg
     9901 ctgttgttgc aactacacag gccaatgggt ttcctccata ggttgaacca tgttctcccg
     9961 gctgaatcgc taacataata ttatcattag ctaaaacagc actcaccggc aacatgccac
    10021 cactcaatgc ttttcccaaa atcaaaatat cgggtttcac gttctcataa tcgcaggcaa
    10081 gcattttacc tgtacgagct aaccctgttt gaatttcatc agcaataaac aaaacattat
    10141 tttgcctgca taatgctgct gctttagaca agtagccatc atcaggaaca acaacaccgg
    10201 cttcgccctg tataggctca acaataaatc ctgcaacgtt agggtcggag agagcatctt
    10261 ccaacgctgc cagattatta tatggaataa ttacatatcc cggagtaaac gggccatagt
    10321 ttgttgatgc tgttgggtct gtagatgcag aaataattga aatagtacgt ccgtgaaagt
    10381 ttccttcaca aaaaataatt tttgcctgat tgcgtgcaat atctttattc ttataagccc
    10441 actttctgca aagcttaatg gctgtttcaa cagcttctgc gcctgaattc atcggcaaca
    10501 gtttatcata gccaaacaac tcagttgcat acttttcgta ttctccaagc ttgtcattat
    10561 aaaaagctct tgacgtaagt gctaactttc ctgcctgctc aactaacgat gccacaatac
    10621 gcggatgaca atgaccctga tttacggcac tgtatgacga taaaaaatca aaatactgtt
    10681 tgccttccgt atcccaaaca aaaatacctt cgccacggct caacactacc ggcaacggat
    10741 gatagttatg tgcaccataa cgctcttcca gttcaataaa ataacttgat cctacagttg
    10801 tttccttact catttccata ataaaaaatt gtctgatgca aatttaatca attgccgcca
    10861 ctttataggt aggcattaga atattacgct gtcaaaacat tcaaacaatc aatatcactt
    10921 cagtcaaaca tatcaaatca aattaaaaca actattttta acgcttcaac aatttacatg
    10981 aaaagaaaaa ctttttctat tgccttgctt ttttgcatca tcagtctgtt ggtttatggt
    11041 tggggatttt ttggtcataa acgaattaac agaatggctg tctttacttt gccagctcaa
    11101 ctaatgcgtt tctacaaagc caacattgat tttattgaag aacatgcagt tgatcccgac
    11161 aaaagacgct atgccgtaaa agacgaagcc gcgcgtcact acattgacct cgaccgctat
    11221 ggcgaatctc ctctcgacag cctgcctaaa aactggaagg ctgctgtcga aaaatacagc
    11281 aaagacaccc tcatggcgca tggcattgtg ccttggcata tcaacgtcat gcttggccgc
    11341 ctcacagatg cattccgaaa caaagactac aacaaaattc tacgctactc tgccgacctt
    11401 ggacactatg ttgccgatgc acatgtgccg ctgcactgca cacaaaacta taacggacaa
    11461 ctcaccaatc aggtgggcat tcatggctta tgggagtcgc gcctgcccga actctttgcc
    11521 gatgattacg actacttcat tggcaaagca caggtcatcg aaaaacctat ggactacatc
    11581 tgggaaatca tcagtcaaag ttttgctgca aaagattctg ttctgttatt cgaaaaagaa
    11641 ctcaatgccc gattcgatgc cgaccgcaaa tatgcctttg agcagcgtgg aaccaccatg
    11701 atgcgtgtct attcagttga ctattccaaa caatatcagg acatgctcaa cggcatggtg
    11761 gaacgcagaa tgcgacaggc aattattgca gtaggcagct tctggctcac agcatggacc
    11821 aatgccggac agcctaacct cgatggtatc gaaaaaatta ccatcagcga ggagatgaaa
    11881 cgcgaaattg aaaacgaaga caaaatgtgg cgcacgggca aagtgctgtc accgggtgga
    11941 catgcagatg atgatcattg attttttgga gtatcaacca atagtttttt attttttctt
    12001 gaagcacaaa ctgcagtaac atctgcaaac gtttctaccg ataactatcg gacttcatag
    12061 ttcagccagt tttaaatcgg taaattgatt agcactgcta tatacataaa aatgtggctc
    12121 tgtaactatt cgtgcttcca caggattgtt gtgtatgtaa tttacttttt gatcaataaa
    12181 tttattactg aataaatcga aggcatgatt atgctgttcc caaaactgat atttaacatt
    12241 gtgcgcattt ttattgccgt aaaactcaaa catataaagc aaccattcgc gcctgctttc
    12301 cgtggcgctt tcttcaatca ttttaatgat ttgctttgtg taactcttaa agtctcgtaa
    12361 catatcgctt aaactcacat cgtcttccgt ttttgcaatt aaatgaatgt gtgatggcat
    12421 aatgcaaaaa gcaaacactt ttagcttttt attgtcaatg ctgtaattta agttcttaac
    12481 tatctcatcg cagtaaatgg ctcttgtaaa aacatcaatc caaccaactg tggttaatat
    12541 aacaaagtat agcccatcag gaaatatttt gtatttttcg cacatgcacc ggcaaagtaa
    12601 gtcttatagt ttgcaagatg taaacaaaat aaatggcacg agcggaaagg acgctcgcac
    12661 ctacgggatt cataagtcaa acgaaaatag aaacaatttt tactttttat ctaaagaatt
    12721 agacttcttt ccttttaaga atgtttttaa ttgatgattg tttccaacca gccacacaat
    12781 atcgccttct tcaaaaatag tattcgatgc agggttagga atgcgttgac catttttttc
    12841 aatacccacc actagtgcat gagctatttc tcttataccg gaatttcgta aactctgctg
    12901 attcagcaga gaatgttttg tcactgtgaa tgtttgtaat ccaatttctt ttgcggcatc
    12961 atcttcttca gtggctggtt cagcgttctc gaatagttct ttgaatttat tcaattgctc
    13021 atcggttccg ataactgaaa ttttatcacc gggataaagc cgctcattcc tgcttggagg
    13081 ggcaattaca tggcttccgc gctctatcat tgcaatgttg attccatact tctcccggaa
    13141 ggaaagttct tccaaagact ttcctataca acttgtttcc ggcactacat caaagtcagt
    13201 catgtgcata tcccagggcg ccataacaga ccgcattgct ttttgctcct cacgctcatt
    13261 aaggttagat aaaaaacgat gctctacacg ttcataaagc ctttgaagat ttcttgaaaa
    13321 gattatcaca gcagttgcta tcgtcaacac cccgacaata aacgcaacaa gaggcgaaaa
    13381 gaaaatctgg aataaaaaac ccaccaagaa aatagctata gcaactctta aaaaatctac
    13441 gattaccaat tgcacacgtc tcactccttt atttatccaa atgcgcatat aggtttccct
    13501 ttttattcgt cttactgcca gtgcccaaat aaaaggaaac ataaatagaa gcgtaacaaa
    13561 cacagtaata atttcaccag agccatttgt ccattctgaa atgagtggat acaaaaaatt
    13621 agacgacaat aaaacaatag cgacaaggat tacagtatga attaatgtgt ttatgccata
    13681 agcacgaagc aaaattttcc agtcgcgtga agctgaagac aattgttgcg caccaacact
    13741 gtagcgattt agacggttca cccatttttc cggtaatttt ttttctagca tattgtaaat
    13801 tacttcggag tgtttaatca gaaaaggggt ggttagagtt gtaacaatag aagcagccac
    13861 tgcaatagga tagagaaaac cacttgtcac ttttaacgtt acacctaatg aagctatgat
    13921 aaaagaaaat tctccgattt gtgccagact taaaccagcc tgaatagagt gttttaacgg
    13981 ctgcccacct aacagcacac caatagcaga ggcaataaat tttccaaaaa ttgtgataag
    14041 ggtgattatc aatactggca ctgcataatc taccaatatc tgagggtcga gcatcatgcc
    14101 aacagaaaca aaaaacactg cggcaaataa attttttaac cggcttggta aggtgttcta
    14161 ttttttctgc ctgcaccgtt tccgcaagta tagaacccat tatgaaagca cccaacgctg
    14221 ctgaaaatcc aacttgtacg gcaaacatca ccatcagcag gcacattgca atcgaaatga
    14281 ttagtagcat ttcatcgttc attaacttgc gtgttttctt taaaaaagta ggaatgaaaa
    14341 aaataccgcc tataaaccat acgatgagaa aaaatatcag cttcaatatc tgtataaaca
    14401 attcactgcc tacaaattct cgacttaccg ccagagtaga aagcaatacc attaacagga
    14461 tggctacgag gtcttcaaca atcagtatcc cgaaaactag tgtagcaaat ttctgtgtgc
    14521 gagcaccaac ttcctcaaat gcccggatga taattgttgt ggaggacata gaaagtattg
    14581 cacctagaaa aatactatcc attaacgacc aactgagtag ttgcccggta ataaatccaa
    14641 ccacaatcat tattacaatc tcaacaatag cagcaataga caccgagccg cctacttttg
    14701 ctaatttttt aaaactaaat tcaagaccaa gagtgaacaa cagaataata actcctattt
    14761 ctgaccaaac tttcacagct tcctcatcag caactgttgg gaatagcgaa atgtgtggac
    14821 ccaccaacaa tcccgcaagt atatatccca gtactagtgg ttgctttatt tttttgaaaa
    14881 taagcatagt aatacctgct acaattaata tgagcccgag gtctgaaatt aaatgaggta
    14941 aatgaaccat tagtaattct catattatgt taactgaaca gagctaacat aaaaatatgg
    15001 gctgcaagat aaaaaaacgt gatgaacttg acttcctgat aatccacgct catcctcgtc
    15061 tgattggctg cattaagggt atgaaacgag aaggttttcc taagcaacca taaaacatat
    15121 ctgtcttggg gtaaaaaatt actttacaca caaaatttgc agcttactaa cagtaaaact
    15181 ttcaaaacat tcttggacaa actatgattg cggcagttgc gataatctgc tcaaagagtt
    15241 gcgaataatt tatatatttg cttcatattg aataaatttg cgaattgtga gtgttgataa
    15301 tataaaagtt atccgtcacc cgtgccggca gtactaactc gagactatct gcaagaattt
    15361 acctttgaca accactataa agtaaaatga taatgaaaaa acgaatcagc ttagcgcttt
    15421 taattttgat tgcaggagtt gtaaacggac agtcaaacaa tcctgcacca aatggttttg
    15481 cgaatttcaa cgagtttagt cataacactc cgtcaatgac tttgaatttt tcagtaaagc
    15541 aacggacgca aggaaatgtt tttatgacag gtggaatcac aaactacaga cttgaaaata
    15601 tccagcctaa gagtgataaa gaaaaattgg aaaaagaagt ttggggagtg atggttcacg
    15661 acactgttta tattaatagt tacccatatt ctaaagttaa aggatacaac caaattattg
    15721 ataaaggtta ttatacttac ttcattggtg aaccagcacg gacagaaaaa gagcagcgtg
    15781 atttaggaat aataaaacca actgaaagtc aaatagcagt ttgttgccaa gcaggatatg
    15841 tgatttcacc agacggaaca attaaacttt tgcgtcctga attattatca gaattgtgta
    15901 aagacaatga agaaataagc aaagagattg cagcagcaaa attgaaactt gaggatgttc
    15961 atcaaatgtt tgcttgtctg aaaaagtata acgctacgaa aaaatagttg tgcaaacttc
    16021 ggacttgtga acttcggaca tacaacttta ttgcgttaat cctttttttg ttaaccaatg
    16081 ccaagagtaa acgacatgaa caataaaaat attttaattt caggtgctgg tattgccgga
    16141 accacattag cattttggtt gaaaaagtac ggttttaatc ctacgattgt tgaaatagct
    16201 ctaaagttaa gagaaggtgg ttacgcaata gattttatgg gtgctggtta cgatgttgcc
    16261 ctaaaaatgg gaattgcaga cgagcttaaa aaggttgaca tagatttttc taaattaaaa
    16321 ttggttgaca aaaacgatca agagaaagga agcatgaact atgagaaagt caaaaattta
    16381 atgaatggga gagccatgac attgctcaga agcgaccttg cgaaaaccat ttataattct
    16441 ttgaacaatg aagttgaaat tatttttgac gatacaattc agcaaataga acaaaatact
    16501 ggtgaagtaa aagtcagttt caaaaatgga aataaaagaa attttgacct cgtgataggt
    16561 gctgacgggt tacattctaa ggtgcgaaac ctcgtttttg gtgatgaaaa ccaatttgaa
    16621 aaatatcttg gctactatac ttcctcatat acaatcaata acttttctat tggcaacaat
    16681 gcattttcaa tgtataatta tccttacaag caagttgcca tttattctaa cagtagaaat
    16741 caaacaactg ctttcttcat ttttagctca gccgaacaac tcacatactc acaacgtgac
    16801 attgaaaaac aaaaacaaat tatgcgacaa gaatttgaaa atggtggatg gaaatgccat
    16861 caattacttt ctgctcttga caccactaca gatttttatt ttgactctgt cagtcagata
    16921 gaaatggaca aatggtcgaa tggcagagtg gcattggttg gagatgctgg ctattgtccg
    16981 tcacttttat ccggcaaggg ttcgaccctt gcaatggttg gagcatatat acttgcaggt
    17041 gagcttaaag aagcaaatgg aaactaccaa caagcatttg aggaatatga aaagcagttc
    17101 aaatcattca tggagaagaa acaaaaatca gcacagtcgt ttgccaaatc atttgttccg
    17161 aaaagtagct tcggaatttg gttaagaaat ttggttttca aactaatgtc aatttcagtt
    17221 ttttcaaaac tatttataaa ccaattcaaa gacattgggc taaaactcaa aaactattga
    17281 aatgcattgg ctataacatc gtatcctatg taaatcccta cactcaggca cgtagataaa
    17341 ttttcttttg aagaggaaaa catttttgaa ttttgccttc ctttttatct cctcagcgtt
    17401 tccacaattt tacaacgggt gactctgaga atgaaggaca ttataaacct tcagacttct
    17461 caaaacgtta ttc
//
LOCUS       DAORQT010000014        17383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_277_length_17383_cov_10.485053, whole genome shotgun sequence.
ACCESSION   DAORQT010000014 DAORQT010000000
VERSION     DAORQT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_17383_cov_10.485053"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..502
                     /locus_tag="PK134_01145"
     CDS             194..502
                     /locus_tag="PK134_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRB37490.1"
                     /translation="MESDSINKNTIFLLLHKNKSQLLKYGVKQLGLFGSFVRNQQNEN
                     SDVDILVEFDEGKKNYKNFLELAYFLEGIFGRKVDLLTPQSLNKYIAPYILKETKYVF
                     "
     gene            492..824
                     /locus_tag="PK134_01150"
     CDS             492..824
                     /locus_tag="PK134_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRB37491.1"
                     /translation="MYFENKELLRHILDEIEFVLFHTNGKSQNTVFNDAVLSRAIIRS
                     LEVIGEASKKIEPDFKTIHPHISWKEIAGTRDKLIHDYFGVDYDIVWDIIVNNLPDLK
                     IQIREVLE"
     gene            980..1363
                     /locus_tag="PK134_01155"
     CDS             980..1363
                     /locus_tag="PK134_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRB37492.1"
                     /translation="MDRRMFYGAKPEIFANAKWLRANMTQSEKLLWSHLKENKLGVRF
                     KPQHPIDIFIADFYCHELKLVIEIDGEIHEKQKEYDAGRTSELQHYDIEVMRFTNAEV
                     MQSIDKVLEQIKIRIDKRRLMDSAL"
     gene            complement(1888..2259)
                     /gene="rplS"
                     /locus_tag="PK134_01160"
     CDS             complement(1888..2259)
                     /gene="rplS"
                     /locus_tag="PK134_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRB37493.1"
                     /translation="MDLVKLAEKQLIPVKDAPDFKAGDTITVFYKIIEGNKEREQQFQ
                     GVVIQRKGHGATQTFTVRKISNGVGVERIFPLFSPKITKIDVNKRGVVRRARIYYLRN
                     VSGKAARIEEKIVKTAETKKK"
     gene            complement(2386..3072)
                     /gene="trmD"
                     /locus_tag="PK134_01165"
     CDS             complement(2386..3072)
                     /gene="trmD"
                     /locus_tag="PK134_01165"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRB37494.1"
                     /translation="MHIDIITVHPGLLEGPFSHSILKRAIQKNLVEINIINLRDYAAN
                     KHKSVDDYAFGGGAGMVMMIEPIDHCITDLKSKRNYDEVIYMSPDGELLNQGISNSLS
                     LKKNIIILCGHYKGVDERVRQHLITREISVGDYVLSGGELPAAVLCDSIIRIIPGVLN
                     DETSALSDSFQDNLLAPPVYTRPANYKGWDVPEVLLSGNESLINEWRHQQSLERTQIR
                     RPDLSDGNSK"
     gene            complement(3075..3896)
                     /gene="mazG"
                     /locus_tag="PK134_01170"
     CDS             complement(3075..3896)
                     /gene="mazG"
                     /locus_tag="PK134_01170"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRB37495.1"
                     /translation="MNHTFAKKMLLAMEEKLNAFKRICTIMDELRTQCPWDKKQTIES
                     LRHLTIEEVYELSDAILDNNLEEVKKELGDIMLHLIFYSRIAEEKNLFNIADVINTEC
                     EKLISRHPHIYPAADGQKTVVADEEEVKRNWEKLKMKEGKISVLEGVPRSLPSMVKAQ
                     RIQEKARGAGFDWEKPEQVWEKVEEEFSEFQEAVSRKNQQEMTDELGDVFFALINYAR
                     FHNLNPDEALERTNRKFISRFRYMEDKVRESGKTLHELSLSQMDVYWNQAKTTEI"
     gene            3888..5006
                     /locus_tag="PK134_01175"
     CDS             3888..5006
                     /locus_tag="PK134_01175"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRB37496.1"
                     /translation="MIHKAGISFLSTLFLFLLTVAAHGQQATHGAWMLGAEGHYGFVM
                     AHRPSVQPLQHSHLGGMMITLSQQADGKRSWHQHFHFPETGIKYGFFGLGNKEQLGYG
                     HTVYPYIDIPLGKSEKPFLHFQFGWGLGYITKPFNRNDNYQNVAIGSHLNAVIAFNFH
                     SEFWLTKRDRIVPAIGLTHFSNGSAAMPNLGINLITAQLHYSRSFGESKEIIRQETEN
                     FEKRNRFTIFGAWAFKQVYPLAGGTWYAWTLSPAFLRQYSPKSAAGISVDLFYDPTIN
                     YRLAYEDMKTHGTISNFRGGVAGAYEIIFSDFSVILQMGVYAKNAWKKDGLFYNRLGM
                     RYRFYDGLFACINLKTHIARADFIEWGIGYNFNKKAWN"
     gene            4997..6232
                     /locus_tag="PK134_01180"
     CDS             4997..6232
                     /locus_tag="PK134_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB37497.1"
                     /translation="MELKEILLWIIASILVLNYLLSMSLSILNLLNLSETQPDELKQY
                     YDEERYKKSQQYEREKSRFGMISETVMFVISFFFLIYGGYGWLDGWTHTVTTDLLWST
                     LLFFGTFALFSDLISIPFSLYSTFVIEERFGFNKTTLAVFISDRIKGYFLAGALGGSL
                     LAMFVLFYQYTGSNFWIYMLAVFAVFMIVLNMFYTSLILPLFNKLTPLADGEVRTAIE
                     NYCQKNKFEITNLYVMDGSRRSSKANAFFSGLGKKKKIVLFDTLVNNYSKDELVAVLA
                     HEVGHYKKKHTLFSMIFSILQTALLLWLLSVFINNEWFSLALGGTEYSLALSLIAFTL
                     LYSPLSLITGILMNIYSRKNEFEADNFARVTFSAEALASALKKLSSDSLSNLTPHPMY
                     VFFYYSHPPLLQRLKALQA"
     gene            6373..7170
                     /gene="cdaA"
                     /locus_tag="PK134_01185"
     CDS             6373..7170
                     /gene="cdaA"
                     /locus_tag="PK134_01185"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRB37498.1"
                     /translation="MELFHIGFLTVRLLDILDVLIVAWVLYKLYRLLKGGVAMNILIG
                     ILTIYFLWWLFVKVLDMQLLGALFGQFIGVGVIALIIVFQQELRRFLIFVGSNTIFTR
                     GDFFKNLLHGNFKLQQPVELSVMPIVRACVSMSKTKTGALIVVTRKTDLNLFEGTGDI
                     MEAEVSKRLLESIFFKNSPLHDGAVIISGNRIRAARCVLPVTESLDVPAHYGMRHRAA
                     LGITEQSDALALVVSEENGQISIAAGGQFYSNLSPDELEKKLKELME"
     gene            7373..8548
                     /locus_tag="PK134_01190"
     CDS             7373..8548
                     /locus_tag="PK134_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRB37499.1"
                     /translation="MPSVSFNNLKVAFAYKSDADLKRAYWLFRLININFLVKIGPGFT
                     NAVLKMGLPIKPLIRATIYKHFCGGETIEQCTETINNLGKYRVGSILDYSVEGHHNES
                     DFDHSCAEIIKTVKHASGKKNIPFSVFKVTGVGRMDLLERVSSKVLLSEAEEAEFLRL
                     RARVESICKTAAENNVRLFIDAEETWIQDAIDNMVDDMMLKYNKEKTLIFNTVQLYRH
                     DRVAFMRENIAKARSGNYRVGYKLVRGAYMEKERERAAVNGYPSPIQPDKAACDHDYN
                     EAIKLCIENIDMVAVCAGTHNEDSSALLAKLMEEKGIANSDERVWFSQLLGMSDHISF
                     NLANAGYCVCKYVPYGPVVSVLPYLFRRAAENTSMSGQMGRELSMITQEIERRKKEK"
     gene            complement(8550..9590)
                     /locus_tag="PK134_01195"
     CDS             complement(8550..9590)
                     /locus_tag="PK134_01195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB37500.1"
                     /translation="MQTDYIIVGNGLAGSLLAWHLLLQEKNVVIIDEFNNTVASQVSA
                     GIFLPITGRRIVRTWMADELLAEVKTIFTQLGKLIGETYLYEMPVVEIPASVKEWNEW
                     QQRASSPDISKYISRFLAPNSIESVHCPFGAIELKNSGYVNISKCVTFLRKLYLDMEI
                     IIEGKFNFDDLQYSDKIVYKDIVADKIIFCEGAAGINNPFFNYLPFQLSKGEILTIRC
                     NTLSEEQIFNHGIFILPIGNQLFRVGSTYEWNDLTTNTTESARNNLSEKLQTVLKSDF
                     EIVDQKAGIRPSVKERRPFIGIHPKHKNIGIFNGLGTKGVMLAPYFAKHFAWHLAHQK
                     PLMADVDIARFS"
     gene            complement(9680..10645)
                     /locus_tag="PK134_01200"
     CDS             complement(9680..10645)
                     /locus_tag="PK134_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37501.1"
                     /translation="MTRFYLSVLAIFSVLTTSAQQMSTDIFLTNFGMNREGQYFFTAP
                     QNITHRPGYDNQPSFSKDGSKVYYVAYYDTLQSDIYVYSLEDSTNTRLTETPESEFSP
                     RVTADDLGFTIVRVDADKGQRFYKVLMDGTNEFQLLGVSDSVAYYCIANDSTYAVADL
                     NNNVMELNIYEMPSEQFIPLAKNIGRCIAVIPDGDNEISYVDKADTNGYMLMSFSINT
                     GLIGSVCQLKKGVEDYVWTKDGKLLIGYEGKLLMFDRNKPDNGWTELADFSKSVGNFY
                     RIAISQQGTQLALVAYTKEDKKQDDAKDDSGKKDDKKKRKKKKDE"
     gene            complement(10642..11262)
                     /locus_tag="PK134_01205"
     CDS             complement(10642..11262)
                     /locus_tag="PK134_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37502.1"
                     /translation="MSLILLMVSPRIPVFFSYIAIALHIIFITLYLIYRNSVHEERVV
                     MLFAAIICSGIIPFTLIVRRNFPLILKLYFGLFMLSLPLFIIAPSKMLTILSMGYLNT
                     ENAHEMHLDKNYYLSKEQGLIKQKDNEATYKVVKRMGIFNKTLVRNIFIGFVPDSAKI
                     LFLDEKSEIRLRAYMHGKTEIDSLDITTKTVASRDTILKIIKTQDK"
     gene            complement(11324..12058)
                     /locus_tag="PK134_01210"
     CDS             complement(11324..12058)
                     /locus_tag="PK134_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRB37503.1"
                     /translation="MLNPKLSDKKTKGILYLIPTAIGSMQAIDYLPQTVIEIIHSLDE
                     FIVENSKSARQFLKHCHIPTPQQQLTIHELNKHEGNVFEASFFSNLLSGKNVGLLSEA
                     GCPAVADPGALIVAEAHKLQIKVVPLTGPSSILLALMASGLNGQQFAFNGYLPKEKTE
                     RFAAIKKAEKDSAQRRQTQIFIETPYRNNALFDDLLNNLQPSTRLCIASALQCENEMI
                     KTQRISDWKKQRQELNKHPVVWLFLA"
     gene            complement(12036..12497)
                     /locus_tag="PK134_01215"
     CDS             complement(12036..12497)
                     /locus_tag="PK134_01215"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRB37504.1"
                     /translation="MVCLGNICRSPLAEGIMQHKLNQAGIAAQVDSAGLINYHTGELP
                     DKRSIAVAAKYGIDLTAQRARSFSKDDIKNFDYIFAMDNKNFENLMEYANNAAEAKRI
                     HLILDYAGLGKKIVPDPYYGDQSDFENVYSLLDDACQRILKKFAYAQSEVV"
     gene            complement(12511..13959)
                     /gene="dnaA"
                     /locus_tag="PK134_01220"
     CDS             complement(12511..13959)
                     /gene="dnaA"
                     /locus_tag="PK134_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRB37505.1"
                     /translation="MLKQTEKNVEKVWENCLKLIRDNVSPQNFKTWFEPIKPVKLEKH
                     VLTIEVPSQFVYEWLEEHYIQLLRKSIKKELGAEGRLEYSILMENNMNSSKPYAVKIP
                     AKGTGNLKNPQVAMPVSLNTAIKNPFIVPGLKKIDVDSQLNPNYSFDNFIEGDCNRLA
                     RSAGYAVANKPGGTSFNPLLLYGGTGLGKTHLAHAIGIEIKNNFQNKTVLYVPADRFM
                     NQFVDAVKNNSPNDFVHFYQMMDVLIIDDIHFLAGKERTQDVFFHIFNHLHQNNKQII
                     LTSDKAPVDLQGIEQRLLSRFKWGLSADLQSPDLETRIAILKRKMYNDGIELPTEIVE
                     YIAYSITTNIRELEGALISIIAQTSLNKKPITLELAKQMIDKFVKNTVHEISIDYIQK
                     IVCDYFELPLETLKSKTRKREVVQARQLAMYFSKSLTKSSLSAIGAHCGGKDHATVLH
                     ACRTVNNLMETDKKFKGYISDLQKRINLNHKN"
     gene            complement(14122..15057)
                     /locus_tag="PK134_01225"
     CDS             complement(14122..15057)
                     /locus_tag="PK134_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRB37506.1"
                     /translation="MTNRRDFLRTFAVGAAGLSLTGLPLEALAKRRVTKITILHTNDV
                     HSHIDPFPANDPKYPGLGGVAQRAAIINDIRNKERNVLLLDAGDIFQGTPYFNLFGGE
                     VELKLMSQLGYDAGTIGNHDFDNGVDGLSSQLKHANFPLIICNYDFKGTSMEGKTIPY
                     KIFEKEDVRIGVFGLGIELEGLVDKKMYGETKYLDPLEKAAETAHHLKFNEKCDLIIC
                     LSHLGYRYPNKKVSDDVLAKKSLNIDLIIGGHTHTFIDKPFVYKNRDNKDVLVAQVGW
                     AGIKLGRIDFFVDKKMRKLTADSTTLKVLNYARAI"
     gene            complement(15047..15805)
                     /locus_tag="PK134_01230"
     CDS             complement(15047..15805)
                     /locus_tag="PK134_01230"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRB37507.1"
                     /translation="MSFRNILLAAAIVFTSCKQNAHLVKTETTTYPLNKPGFTEVDSS
                     ILNSYSSYKHTIDSIMDRVIIVSEMSMQKKTPEGLLGNFTSDAALKQVENDFHAKDFS
                     KIDFCFLNNGGLRAALPKGDITIGNIYSLMPFENEMVLLTLNGATTLKLINFIAAKGG
                     MPVSGLTMRVHESTVTEAKIQGMPFDSTKSYVVATSDYLANGGDNLSFLADAEKKVST
                     GLKLRDLLIQYLEEKNKRGEKLSAKIEGRIIYDK"
     gene            16021..16524
                     /locus_tag="PK134_01235"
     CDS             16021..16524
                     /locus_tag="PK134_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37508.1"
                     /translation="MHPHFKLLLAVFVLFSISSQAQRIDKSFRIKVSDNRNLQFTSYE
                     YLLKDDSMKISGVSDYGKSRVVYIAKKLKSKQKKAIRNFLKDFPVDSLQKEYFDDYAN
                     FGYIASDHFPRVIELEIRTGLKQHFSKASNAYVRSYARLIDFLNTLINDDVVKINYPE
                     SDFKKTF"
     gene            16528..>17383
                     /locus_tag="PK134_01240"
     CDS             16528..>17383
                     /locus_tag="PK134_01240"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRB37509.1"
                     /translation="MRTKTSKQLKVNVVTLGCSKNVVDSEVLMGQLRANEIEVEHESK
                     RQSEIVIINTCGFIDNAKQESIDTILHYAKEKEKGKIEKLIVTGCLSERYKPDLEKEI
                     KGIDNIFGTRDLPLLLKTLGADYKHELIGERLVTTPRHFAYMKISEGCDRPCSFCAIP
                     LMRGNHISKPIEELVHEARHLAAKGTKELLLIAQDSTFYGLDIYKERKLANLMEQLCS
                     VDGIEWIRLHYAFPSGFPLDVLDIMQRENKICKYLDIPLQHASDNMLKSMRRGITREK
                     TITLLDTVR"
BASE COUNT         5346 a         2957 c         3315 g         5765 t
ORIGIN      
        1 cagacacccc ttcaggggtt gggggtgagc ggaggaatgc agattttttc tgaataatga
       61 agtacttaga taatttgata attaggtaat ttgataattt gataattcag agtgcttgcg
      121 cctacttagg tcaatttgta cacagaacta ttttacattg ttttttgatt cataaattat
      181 ttaattttga aaaatggaat cagattctat aaataaaaac accatttttt tgctcctgca
      241 taaaaacaaa tcacaattgt tgaagtatgg agtaaagcaa cttggattat ttggctcgtt
      301 tgttcgaaat caacagaatg aaaacagtga tgtagatata ttagtagagt ttgatgaagg
      361 taaaaaaaat tataaaaatt ttttagagct tgcctatttc cttgagggta tatttggacg
      421 taaagttgat ttattaaccc ctcaatcatt gaataaatat atagctcctt atattttaaa
      481 agaaacaaag tatgtatttt gaaaacaagg aattgttaag acatattttg gatgaaatag
      541 aatttgttct ttttcacacg aatggtaaat cgcaaaatac cgtttttaac gatgctgtac
      601 tttcaagagc aattatcagg agtcttgaag taattggtga ggcatcaaaa aaaattgagc
      661 ctgactttaa aacaatacat cctcatataa gttggaaaga aatagcagga acacgcgaca
      721 agttaattca tgactacttt ggagttgatt acgatattgt ttgggatatt attgtaaata
      781 atttacctga cttgaaaatt caaatcaggg aggtgttgga ataagatttt tgataattca
      841 gagttagcag ttaagaatta agaaataaga attcagagtg cttgcacgca cccgtgacca
      901 aaagaggcgt tgctctttgc aaaatgcctg aagggttcct gcacacaaat atttttttaa
      961 ttagaataga tttaatgtta tggatagaag gatgttttat ggcgccaagc ctgaaatttt
     1021 tgctaatgca aaatggcttc gtgcaaatat gacacaaagt gaaaaattgt tatggagtca
     1081 tttgaaggaa aataaattag gagttagatt caagccgcaa catcctatag atatttttat
     1141 tgctgatttt tattgtcatg aattaaaact ggttatagag attgatggtg aaattcatga
     1201 aaaacaaaaa gaatatgatg cagggagaac ctctgaattg caacattatg atattgaagt
     1261 tatgcgtttt acaaatgctg aggttatgca atcaattgat aaagtacttg aacaaataaa
     1321 aatcagaata gataaacgaa gattgatgga tagcgccctt tagggctacg ggtgagccga
     1381 ggagaaagga gttcgttttt ctgaataatg aagtacttag ataatttgaa aattaggtaa
     1441 tttgataatt tgataatgca gagtgcttgc actcactcgt aaccaaaaga agctttactc
     1501 taattagaat agatttaatg ctatggatag aaatatgttt agcggacaga caccccttca
     1561 ggggttgggg gtgagcagag gaatgcagat tttttctgat tgatgaagta cctagagaat
     1621 ttgaaaatta ggtaatttga taatttgata attcagaatg cttgctccta cttaaaatag
     1681 caatgctaat caatttaccg atttaaagct ggctgaacta tgaagtccga tagctatcgg
     1741 acggccagag gccgcaagga taaaaggcac gagcggaaag gacactcgcg cctacttagc
     1801 tcgcgcctac ttagtctttg ggaaagtgta ctcataaaaa aagccccgtt attacgaggc
     1861 ttctttacaa atcaaaatga aactttttta ttttttcttg gtctcagctg tcttaacaat
     1921 tttttcttct atacgcgcag ccttacctga aacattacgc aggtaataga ttcttgctct
     1981 gcgaacaaca ccgcgcttgt ttacatcaat cttggtgatt ttaggactga ataatgggaa
     2041 aatacgctca acgcctacac cattagaaat tttacgaacg gtaaatgtct gtgttgcacc
     2101 atgtccttta cgttgaataa caacaccctg aaactgctgc tcacgctctt tattaccttc
     2161 aataatttta tagaatacgg taatggtatc accggctttg aaatccggag catcttttac
     2221 cggtattaac tgcttttctg ctaatttaac taagtccatc gctttatatt ttgatgattg
     2281 ataaaatttt gggagtgcaa taatagcaaa aaaagcttta gggtgataag atttatacta
     2341 actttttaaa aaatgtttaa aaaattgtat tttattgaaa aacagttatt tgctattgcc
     2401 atctgacagg tctggtcttc tgatttgtgt acgttccagg ctttgctgat gccgccattc
     2461 attaataagg ctttcattac ccgacaaaag cacttcagga acgtcccagc ctttgtaatt
     2521 tgcgggtcgg gtataaacag gcggtgccaa aaggttatcc tgaaaagagt cggacagggc
     2581 agaggtttca tcattcaaaa cgccaggaat aattctgatg atgctatcac aaagtacggc
     2641 tgcaggtagt tcgccaccgg aaagaacata atcgccaaca gaaatttcgc gtgtaataag
     2701 atgttgccgc actctttcat caacaccttt gtaatgacca cagaggataa tgatattttt
     2761 tttcaacgac aggctattgc ttattccctg attgagtagt tcgccatcgg gcgacatgta
     2821 gatgacttca tcataatttc tttttgattt aaggtctgtg atacaatggt caataggttc
     2881 tatcatcatc accattccgg caccaccgcc aaaggcatag tcatcaacac ttttatgttt
     2941 gttggcagca tagtctcgaa ggttaatgat atttatttca acaagatttt tctgaatggc
     3001 tcttttaaga atagaatgac tgaaaggccc ttcaagcaaa ccgggatgta ctgtgataat
     3061 atcaatatgc atcatcagat ttcagtggtt tttgcctggt tccaataaac atccatttgt
     3121 gacagtgaga gttcatgtaa agtttttcct gattctctga ctttgtcttc catatatcta
     3181 aaacgcgaaa taaattttct gttcgtgcgc tccaatgctt catcgggatt cagattgtga
     3241 aaacgtgcat agtttatcag tgcaaaaaaa acatcaccca attcatcggt catttcctgt
     3301 tgatttttac gggagacagc ttcctgaaat tcactgaact cttcttcaac tttttcccat
     3361 acctgctccg gtttttccca gtcgaagccg gcaccacgag ctttctcctg aatgcgctgc
     3421 gcctttacca tgctgggtaa tgaacgaggc acaccttcca atacagatat cttgccttct
     3481 ttcattttca atttttccca gttacgtttt acttcttctt catcggcaac aacagttttt
     3541 tgtccatcgg cagcaggata aatatgtggg tggcgcgaaa tcagtttctc acattctgta
     3601 ttaatcacat cagcaatgtt aaacaggttt ttttcttcgg caattcgtga ataaaaaatc
     3661 aaatgcaaca taatgtcgcc caattctttt ttaacctctt ctaaattgtt atctaagatg
     3721 gcatcactca actcataaac ctcttcgatg gtgagatgtc gcaacgattc tatggtttgc
     3781 tttttatccc acggacactg tgtgcgcagt tcgtccatga ttgtacaaat tcttttaaaa
     3841 gcatttaatt tttcttccat tgctaaaagc attttctttg caaaggtatg attcataaag
     3901 caggcattag ttttctgtcc acactatttt tattcttgct gactgttgca gcacatgggc
     3961 agcaagccac acatggtgca tggatgctgg gtgcagaggg tcattatggt tttgttatgg
     4021 cacaccggcc ttctgttcag ccactacaac acagtcatct tggtggtatg atgattacct
     4081 tatcacagca ggcagacggc aaacgatcat ggcatcagca ttttcatttt ccggaaacag
     4141 gaattaaata tggttttttc ggattgggta ataaagaaca gttaggctat gggcatacag
     4201 tatatcctta cattgacatt ccattaggca aatcagaaaa accattttta cattttcaat
     4261 tcggatgggg attgggatat attaccaaac cgtttaacag aaatgataat tatcagaatg
     4321 tagctatagg atcgcatctg aatgctgtta ttgcatttaa ctttcactcg gagttttggc
     4381 ttactaaacg cgacagaatt gttcctgcca taggtttaac acatttttca aatggaagtg
     4441 cggcaatgcc aaatctggga ataaatttaa ttacggcaca acttcattac agccgtagtt
     4501 ttggtgaatc aaaagagatt atcaggcaag aaactgaaaa ttttgaaaaa agaaatcgct
     4561 ttacaatttt tggagcatgg gctttcaaac aagtttatcc gcttgccgga ggcacctggt
     4621 atgcatggac tctttctcct gcatttttaa ggcagtacag ccctaaatct gcggcaggca
     4681 taagtgttga tttattttat gacccgacaa ttaattacag acttgcgtat gaagacatga
     4741 aaacacatgg aacaatatca aattttcgtg gaggtgttgc aggagcgtat gaaataatat
     4801 tttcggattt ttctgtcata ttgcagatgg gtgtttatgc aaaaaatgca tggaagaaag
     4861 atggtttgtt ttataacaga ctgggaatgc gctatcgttt ttatgatgga ttgtttgcct
     4921 gcatcaacct gaaaacacat attgcaagag ccgattttat agagtgggga ataggatata
     4981 actttaataa aaaagcatgg aattaaaaga aatattgttg tggataattg catcaatact
     5041 ggtgctgaat tatctgttga gtatgagttt gtctatttta aatttattaa acttatcaga
     5101 aacacaaccc gatgaactga aacaatatta tgatgaagaa cggtataaaa aatcacagca
     5161 atatgagcga gagaaaagcc gcttcggaat gattagtgaa acggtcatgt ttgttatttc
     5221 tttctttttt ctgatttatg gtggttatgg ttggcttgat ggttggacac atacggtaac
     5281 tacagatttg ttatggtcca ccttgttgtt ttttggaact tttgctttgt tcagtgattt
     5341 aataagcatt ccgttttcac tttattcaac ctttgttatt gaagaacgtt ttggatttaa
     5401 taaaacaaca cttgctgttt ttatttcaga caggattaaa ggatattttc ttgcaggagc
     5461 attaggtgga agtttgttgg ccatgtttgt tttgttttat caatatacag gaagtaattt
     5521 ttggatttac atgcttgcag tttttgcggt ttttatgatt gtattgaata tgttttacac
     5581 ctcattgata cttccattgt ttaataaact tactccattg gcagatggcg aagtacgtac
     5641 tgcaattgag aactactgtc agaaaaataa atttgaaatt actaaccttt atgtgatgga
     5701 tggtagcaga cgttcatcaa aggcaaatgc ctttttcagt gggttaggca aaaagaaaaa
     5761 gattgttttg tttgatacct tggttaataa ttattcgaaa gatgagttgg ttgcagtgct
     5821 ggctcatgaa gtaggtcatt acaagaaaaa gcatacactg ttttcaatga tattttcaat
     5881 ccttcaaact gctttgttgc tgtggctgct ttctgtattt atcaataatg aatggttttc
     5941 tttagctctt ggcggaacag aatacagtct ggctttaagt cttattgcat ttactttatt
     6001 gtattcaccc ttatcactaa ttacaggaat cttgatgaat atctattcca gaaaaaatga
     6061 gtttgaagcg gataattttg caagagtaac attcagtgca gaagcattag caagtgcatt
     6121 aaaaaagctc agttcagata gtttgtcaaa tcttactccg catcccatgt atgtattttt
     6181 ctattactca catccgcctt tgttgcagcg attaaaagca ttgcaagctt agttttatac
     6241 agaatgattt ttatttttta gaactatagg taatagttca aaatgaagtg tcaacaacat
     6301 tgtgcgaaaa aaattatgaa acaacagtaa agttgaaata aacttctgtt atttgtaatc
     6361 atagcgacag gcatggagtt gtttcacata ggatttttaa ccgtaagact gcttgatata
     6421 cttgatgtat taattgtagc atgggtgctg tataaacttt atcgtttact aaagggaggt
     6481 gttgcaatga atatcctcat aggcattctt accatatatt ttctgtggtg gctttttgtg
     6541 aaagtactcg acatgcaatt gcttggtgct ttgtttgggc agtttattgg agttggagtt
     6601 attgcactta ttattgtttt tcagcaggaa ctacgcaggt ttttaatttt tgtaggctca
     6661 aatactattt ttacaagagg tgattttttt aagaacctgt tgcatggaaa tttcaagctg
     6721 caacaacctg ttgagttaag tgtaatgcct attgttcgtg cctgcgtttc aatgtctaaa
     6781 acaaaaacag gagcgttgat agtagtgacc agaaaaaccg atttgaattt gtttgaaggt
     6841 accggtgata ttatggaagc tgaagtttcc aaacgacttt tagagagtat attttttaaa
     6901 aacagcccct tgcatgatgg tgctgtaatc atcagtggca acagaattcg tgctgcccgc
     6961 tgtgttttgc ctgttactga aagtttagat gtgcctgcac attacggaat gcgccacaga
     7021 gctgcattag gtatcacgga gcagagtgac gctttagcgt tagttgtgtc ggaagaaaac
     7081 gggcagattt ctatagctgc aggcggacag ttttactcca acctttcacc ggatgagttg
     7141 gaaaagaaat tgaaagaatt gatggagtga ggttgtactg ctttcatata aacaaaccat
     7201 tcttgtcttt tttctcgaat aactttccaa acacattcca atagtttgac aaattggtta
     7261 aagtaaggtt aaatcaaagt gttaagctgt tgattaatat catatttacc tcttacagaa
     7321 attagattta cggatatttt aattcaatta tctttggcaa agaattttta atatgccatc
     7381 tgtatcattc aacaatctga aggttgcatt tgcttataaa agcgatgcag atcttaaaag
     7441 agcctattgg ttattcaggc tcatcaatat taactttctc gtaaaaattg gtccgggatt
     7501 caccaatgca gttttaaaaa tgggtttgcc gataaagcca ttgataagag ctaccattta
     7561 caaacatttt tgtggaggag aaaccattga gcaatgtact gaaacgataa ataatctcgg
     7621 aaagtataga gtaggctcaa ttttggatta ttctgtagaa ggccatcaca atgaatctga
     7681 ttttgaccat agctgtgcag aaattattaa aaccgtaaag catgcaagcg gaaagaaaaa
     7741 tattcctttt tctgtattta aagttaccgg tgtaggcagg atggatttgt tagagcgtgt
     7801 cagcagtaag gttttacttt cagaagccga agaggcagaa tttttgagat taagagcaag
     7861 agttgaatca atttgtaaga cagcagcaga gaataatgtg cgtttattta ttgatgcaga
     7921 agaaacatgg attcaggatg ccattgacaa catggttgat gacatgatgt tgaaatacaa
     7981 caaagagaag acacttattt ttaataccgt tcagctttat cgtcatgaca gggttgcatt
     8041 tatgagagag aatattgcaa aagcccgttc aggtaattac agagtagggt ataagttagt
     8101 gcgcggtgcc tatatggaaa aagaaagaga acgcgcagca gtaaatggtt atccttctcc
     8161 tatacagcca gataaagcag cctgtgatca cgattacaat gaggctataa agttgtgcat
     8221 tgaaaatata gacatggttg ctgtttgtgc cggcacacac aatgaagata gcagtgcctt
     8281 acttgcaaaa cttatggaag aaaaaggtat tgcaaattct gatgagcgtg tttggttttc
     8341 gcaactgctt ggcatgagcg atcacattag ttttaatctt gctaatgcag gttactgtgt
     8401 ttgtaagtac gtgccttatg gacctgtagt ttctgtgctg ccttatttat tcagacgtgc
     8461 cgctgagaat accagcatga gtggtcagat gggacgcgaa ttgagtatga ttacacaaga
     8521 gattgaaaga cgtaagaaag aaaaatagtt taagaaaaac gagctatatc cacatcggcc
     8581 attaacggtt tttgatgtgc aagatgccag gcaaaatgtt ttgcaaaata aggagcaagc
     8641 atcacacctt tagtgcccag cccattaaat attccaatgt ttttatgttt tggatgtatg
     8701 cctataaaag gtcttctctc ttttacactt ggtctgatgc ctgctttctg atcaacaatt
     8761 tcaaaatcgg actttaatac tgtctgcaac ttttcagata gattatttct ggcactttct
     8821 gttgtgtttg ttgttaaatc attccattcg taagttgaac ccactctgaa tagctgattc
     8881 cctattggca gaataaaaat accatgatta aaaatctgct cttctgaaag tgtattacaa
     8941 cgtattgtca gaatttcacc tttggagagt tgaaagggca gataattaaa aaatggattg
     9001 tttataccgg cagcaccttc acagaaaata attttatcgg ccacaatatc tttataaact
     9061 attttatcgg aatactgtaa atcatcaaag ttgaattttc cttcaatgat aatttccata
     9121 tctaaataaa gtttccttaa aaaggtaaca catttagaaa tgtttacata gccgctgttt
     9181 tttaattcta ttgctccaaa tgggcaatga acagattcta ttgaattagg tgctaaaaac
     9241 cggctgatgt atttgctgat gtcaggactt gatgcacgct gttgccattc attccactct
     9301 ttaacagaag ccggaatttc aactacaggc atttcatata aatatgtttc accgattaat
     9361 tttcctaatt gcgtgaagat ggtttttact tcagcaagaa gctcgtcagc catccatgtc
     9421 cttacaatgc gtcttccagt aatcggcaaa aaaattcctg ctgaaacctg tgatgcaaca
     9481 gtgttgttga actcatcaat aataactaca tttttttctt gcaataaaag atgccaggca
     9541 agcaaactac ctgctaaacc atttcctact atgatataat cagtttgcat gagagaaggt
     9601 tagtttttgt tatagaagta cttacgtcca gaaagaatta ggagttaagt gctaattata
     9661 ttcagcaacg aatactgttt tattcatctt tcttcttttt gcgttttttc ttgtcgtctt
     9721 ttttgccgga atcatcttta gcatcatcct gttttttatc ttccttagta taggcaacca
     9781 atgcaagttg agttccttgt tggctgattg caattctgta aaaatttcca acagatttac
     9841 tgaagtctgc tagctcagtc catccattat caggcttgtt tctgtcgaac atcaataatt
     9901 ttccttcgta accaataagt agtttaccat cttttgtcca tacataatct tcaacacctt
     9961 ttttaagttg gcatacactt ccaatcaatc ctgtgttaat tgaaaatgac atcagcatat
    10021 agccattggt gtctgctttg tcaacataac ttatttcatt atcaccgtct ggaatgactg
    10081 caatacaacg accaatattt tttgccaatg gaataaattg ttcactcggc atttcgtaaa
    10141 tatttaattc cattacattg ttgtttaagt ctgcaacagc ataggtgctg tcatttgcaa
    10201 tgcagtaata ggcaacagaa tcagatacac caagaagttg aaactcattt gtgccatcca
    10261 tcagcacttt ataaaaccgc tgacctttat ctgcatcaac tctcacaata gtaaatccta
    10321 aatcatcagc agtaacccga ggagaaaatt cactttcagg agtttctgtc agccttgtgt
    10381 ttgtgctatc ttcaagacta taaacataga tgtcgctctg caaagtatcg taataggcta
    10441 cataataaac tttactgccg tctttagaga acgagggctg attgtcgtag cccggacgat
    10501 gtgtaatgtt ctgcggagct gtaaaaaaat attgcccttc gcggttcatg ccaaagtttg
    10561 tcagaaaaat atctgttgac atttgttgtg cagatgttgt cagtactgag aatattgcaa
    10621 gtactgataa atagaatcgt gtcatttatc ttgagtttta atgattttta atatggtgtc
    10681 gcgtgatgct acggtttttg ttgttatatc taaactatca atttctgttt taccgtgcat
    10741 gtaagcacgg aggcgaattt ccgatttttc gtctaaaaat aaaatttttg cagaatcggg
    10801 cacaaaacca ataaaaatat tgcgcacaag ggttttatta aagatgccca ttctttttac
    10861 aactttatag gtagcttcat tatctttttg tttaattaag ccttgctctt tagagaggta
    10921 atagttcttg tcaaggtgca tttcatgtgc attttctgtg ttcagatagc ccatactcag
    10981 aatagtgagc attttagatg gtgctatgat aaacagtggt aaactcagca taaaaagacc
    11041 aaagtataat tttaatatta gcggaaaatt tcgtctgaca atgagcgtaa acggtatgat
    11101 gccactacaa attattgcag caaacagcat gactactctt tcttcatgga ctgaatttct
    11161 ataaatcaaa tacagcgtaa tgaaaattat atgcagtgca atggcaatat aactaaaaaa
    11221 aacagggatg cgtggactta ccatgagtaa aatcaatgac actacaaagc atgatattat
    11281 ggtaatgagt agaaaattca gcagcgcaac cattcaataa atattaagcc aaaaatagcc
    11341 aaactacagg gtgtttgttt aactcctgtc tttgtttttt ccagtcagaa attcgctgtg
    11401 ttttaatcat ttcattttca cactgcagtg cagaggcaat gcaaagacgc gttgaaggtt
    11461 gcagattatt tagtaaatca tcaaacaagg cattgttgcg atagggtgtt tcaataaaga
    11521 tttgtgtttg tctgcgttgt gcagaatctt tctctgcttt ttttattgca gcaaaacgtt
    11581 ctgttttttc ttttggcaga tagccattaa aagcaaactg ttgcccattg agtcctgaag
    11641 ccattaatgc taaaagtata gatgatggac ctgtaagcgg aactactttt atttgtagtt
    11701 tatgtgcttc ggcaacaatt aatgcacccg ggtcggcaac agcagggcag cctgcttcgc
    11761 taagcaggcc aacatttttt ccggaaagca gattgctgaa aaacgatgct tcaaaaacat
    11821 tgccttcatg tttattgagt tcatgaatgg tgagctgctg ttgtggtgtt gggatgtgac
    11881 aatgtttaag aaactgtctt gcagattttg aattctctac tataaactca tccaatgaat
    11941 gaattatttc aattacagtt tgtggcaaat aatctattgc ctgcatactg ccaattgcag
    12001 taggaataag gtataaaatt ccttttgttt ttttgtcaga caacttcgga ttgagcataa
    12061 gcaaactttt tcaaaatgcg ttggcaggca tcgtcaagta agctatacac attctcaaaa
    12121 tcactttgat cgccatagta gggatcggga acaatctttt tgcccagacc tgcatagtct
    12181 aatataagat gaatgcgttt tgcctctgct gcattattag catattccat caggttttcg
    12241 aagtttttgt tatccattgc aaagatgtaa tcaaaatttt taatgtcatc tttgctaaaa
    12301 ctacgtgccc tttgagcagt aaggtcaatg ccatatttgg cagcaactgc tattgagcgt
    12361 ttatcaggta attctcctgt atgatagttt atcaaacctg ccgaatcaac ctgtgcagca
    12421 atacctgcct gattgagttt atgttgcatt atgccttcgg caagtggtga gcggcagatg
    12481 ttgccgaggc aaaccatcag aattttcacc ttaatttttg tgattaagat ttattcgctt
    12541 ttgcagatcg ctgatatagc ctttgaattt tttatcagtc tccatcaggt tgttcactgt
    12601 gcggcaggca tgtagaactg ttgcatggtc tttgccacca caatgtgcgc caattgctga
    12661 aagcgatgat tttgtcagac tcttagaaaa atacattgct aactgacgtg cctgtactac
    12721 ttcacgcttg cgtgtttttg actttaatgt ttctaaaggc agttcaaaat aatcacaaac
    12781 tattttttga atatagtcaa tggaaatttc atgcacagta tttttcacaa acttgtcaat
    12841 catttgtttg gcaagttcga gagtgatagg ctttttattt aatgatgtct gtgcaatgat
    12901 agaaatgagt gccccttcaa gttctctgat gtttgtagta atactataag caatgtattc
    12961 aacaatttca gttggcaatt caattccgtc attatacatt ttccttttca ggatggcaat
    13021 gcgtgtttct aaatctggcg actgcaagtc agcagataaa ccccatttaa aacgtgatag
    13081 taaacgttgt tctattccct gtaagtcaac aggtgcttta tcagatgtaa gaattatctg
    13141 tttgttattc tgatgcaggt gattaaagat gtggaagaat acatcttgtg ttctttcttt
    13201 gcctgcaagg aaatgaatgt cgtcaataat taaaacatcc atcatctgat aaaaatgtac
    13261 aaagtcgttt ggtgaattgt ttttaactgc atccacaaac tgattcataa acctgtcggc
    13321 aggaacataa agtacagtct tattctggaa attatttttt atttcaatac cgatggcatg
    13381 agcaagatgt gtttttccta atcctgttcc accatataat aataatggat taaaggaagt
    13441 accaccgggt ttgttggcaa ctgcataacc tgcagatctt gcaagacggt tgcaatcacc
    13501 ttcaataaag ttgtcgaaag aatagtttgg attcagttgt gagtcaacat caatcttttt
    13561 tagtcccgga acaatgaatg gattttttat agctgtgttt aagctaaccg gcattgctac
    13621 ctgtggattc tttaagttgc ctgtcccttt agcaggaatt ttcactgcat aaggttttga
    13681 gctgttcatg ttattctcca tcagaataga gtactccaag cgaccttcag cacctaattc
    13741 ttttttaatg ctcttgcgca acagttgtat ataatgctct tcgagccatt catatacaaa
    13801 ctggcttggt acttctatag tcagtacatg cttttctaat ttaaccggtt tgatgggttc
    13861 aaaccatgtt ttaaaatttt gtgggcttac attgtcgcgt ataagcttca aacaattttc
    13921 ccacactttt tcaacatttt tctctgtctg tttcaacact aatcgtaatt ttaaaagtta
    13981 agtattggtt tgaggtaaaa tttggttttt aaagatagaa gtaaattttc aattgtaaga
    14041 taaaattcaa aaattatttt ttggtggttc tcgtttttta atcgaggtgc aaatatgctt
    14101 tgaatattct tgaaaaaaaa attaaattgc tcttgcataa ttcaaaactt ttaacgtggt
    14161 actgtcggct gtcaatttac gcattttttt atcaacaaaa aaatctattc tgcctaattt
    14221 tattcctgcc caacctacct gagccactaa aacatcttta ttgtcgcgat ttttatacac
    14281 aaagggtttg tctatgaatg tatgtgtgtg accaccaatt attaaatcta tgttgagcga
    14341 tttctttgca agaacatcat cacttacctt tttgtttgga tatctatagc ctaaatgtga
    14401 gagacaaata attaaatcgc acttctcatt gaattttaaa tgatgcgcag tttctgctgc
    14461 tttttccaag gggtcaagat attttgtttc accatacatt ttcttgtcaa ccaagccttc
    14521 cagttcaatg ccaagtccga aaactccaat acgtacatct tctttttcaa aaattttata
    14581 tggtattgtt ttgccctcca ttgaagtgcc tttgaaatcg taattacaaa ttatcagagg
    14641 gaaattggca tgtttcaact gacttgatag accgtcaaca ccattgtcaa agtcgtgatt
    14701 gccaatagta ccggcatcat atccaagttg cgacattaat tttaattcta cctcgccacc
    14761 aaataaatta aagtaaggtg tgccctgaaa aatatcaccg gcatcaagaa gaagtacatt
    14821 gcgttcttta tttctgatat cattaataat agcagcacgt tgtgctactc cgccaagtcc
    14881 cggatatttt ggatcgttag ccggaaatgg gtctatgtgg ctgtgaacat cattggtatg
    14941 gagtatagtt atcttagtaa ctcttctttt agccaaagcc tcaagtggta atcctgtgag
    15001 actgagtccg gcagcgccca ctgcaaatgt tcttaaaaag tctcttctat ttgtcataaa
    15061 taatcctccc ttcaatttta gcagataatt tttctcctcg tttatttttt tcttccagat
    15121 attgaattag caggtcgcga agttttaatc cggtagagac tttcttttca gcatcagcta
    15181 aaaaagaaag attgtcacca ccattggcaa gataatctga tgtagccaca acataacttt
    15241 ttgtactgtc gaatggcatt ccttgaattt tagcttcggt tacggttgat tcatgtacgc
    15301 gcattgttaa tcctgaaaca ggcataccac ctttagcagc aataaaattg ataagtttta
    15361 aagttgttgc accatttaat gtaagcaaaa ccatttcatt ttcaaaaggc attagtgagt
    15421 agatgttgcc aattgtaata tctccttttg gcaatgcagc acgcaaacct ccattattca
    15481 aaaagcagaa atctatttta gaaaagtctt tagcatggaa atcgttttct acttgtttta
    15541 atgctgcatc agaagtgaaa ttgccaagta aaccttcggg ggtttttttc tgcatgctca
    15601 tttcactcac aataatcaca cgatccatta tggagtcaat agtgtgtttg taagatgagt
    15661 aagagtttaa gattgaggaa tcaacttcag taaagcccgg tttattaaga gggtaggtag
    15721 tggtttctgt ttttactaag tgtgcatttt gtttgcatga tgtaaataca attgcagcag
    15781 caagtaaaat atttcggaaa gacatactgg taaaggttct caaaattgaa tgctaaaagt
    15841 aatgaaccaa attatacgaa gtagttaaaa aaaaatattt tttttcggag tagtattttt
    15901 caatcctttt tttattttat gcaattttca tagtgcaaag gttaaatagt agtttcattg
    15961 ctatgcaagt gcagaattga taactttgca ccggtataaa gaactcaata tcattatttc
    16021 atgcaccctc actttaaatt attacttgct gtatttgttt tgtttagtat aagcagtcag
    16081 gcacagcgca ttgacaaatc tttccgtata aaagtttctg ataacagaaa cctgcagttt
    16141 acctcttatg aatatttgtt aaaggatgac agcatgaaaa tcagtggtgt ttctgattat
    16201 ggaaaatcgc gtgtagtata tatagcaaag aaattgaaat caaaacaaaa gaaagcaata
    16261 agaaactttt tgaaagattt ccctgttgac tcgctgcaga aagaatattt tgatgattat
    16321 gcaaacttcg gttatatcgc atccgatcat tttccgagag taattgagct tgaaataaga
    16381 acagggttga aacagcattt cagcaaagct tcaaatgcct atgtgagaag ttatgcaagg
    16441 ctgatagatt ttctcaatac actgattaat gatgatgtgg ttaaaatcaa ttatccggaa
    16501 tctgatttta aaaaaacatt ttagtctatg agaacaaaaa catccaaaca gttaaaagtt
    16561 aatgttgtta cgttaggttg tagcaaaaac gttgttgact ccgaagtgtt gatgggtcag
    16621 ttgcgtgcca atgaaataga ggtagaacat gaatcgaaaa gacaatcaga aattgtaatt
    16681 ataaatacct gtggatttat tgataatgca aaacaggagt caatagatac aattctacat
    16741 tacgcaaaag agaaggagaa agggaaaatt gaaaaattga ttgtcaccgg atgtctttct
    16801 gaaagataca aacccgattt agaaaaagag ataaagggta ttgacaatat ttttggtaca
    16861 cgcgatttgc cattgctgct aaaaacttta ggagcagatt ataagcatga attaatagga
    16921 gagcggttgg ttacaacacc tcgtcatttt gcctacatga aaatttctga aggttgcgac
    16981 cgtccttgtt cattctgtgc cattccgctt atgcgaggca atcacatttc aaaaccaatt
    17041 gaagagttgg ttcatgaagc aagacactta gctgcaaaag gcacaaagga attactgctt
    17101 atagcacaag actctacctt ttatggttta gacatttaca aagagagaaa acttgcaaac
    17161 ctgatggagc agttatgctc tgttgatggt attgaatgga ttcgcctgca ctatgcattt
    17221 ccctctggat ttccgctcga tgtgcttgat ataatgcaaa gagaaaataa aatttgcaag
    17281 tacttggata ttccacttca acatgcaagt gacaatatgt taaagagcat gcgtagagga
    17341 attacacgcg aaaaaacaat tactttatta gacacagtgc gta
//
LOCUS       DAORQT010000015        17303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_281_length_17303_cov_17.618217, whole genome shotgun sequence.
ACCESSION   DAORQT010000015 DAORQT010000000
VERSION     DAORQT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_17303_cov_17.618217"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK134_01245"
     CDS             complement(<1..627)
                     /locus_tag="PK134_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HRB37510.1"
                     /translation="MQKIVKLYEWTTSLPIITCLLYFSGLLDNNIVLQILAGILLLFC
                     VMSAVHHSEIVAHRVGEPYGTIILAVSITIIEVAIIISLMMTGGKEYASFARDTVYAA
                     VMLILNGIIGLSLFLGGRKYHTQTFSPHSVKIALVALVSIVTFTMILPTFTSSIIGPY
                     YTERQLLFEIIACLIIYIAFIAAQTIRYREYFITEANPDVEVTHNNISN"
     gene            980..2740
                     /locus_tag="PK134_01250"
     CDS             980..2740
                     /locus_tag="PK134_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37511.1"
                     /translation="MKILVTYLKRHWKLVLLALLLAAVNQCFSLIAGKMLDRFAMHVS
                     NYSNDFPKFFQDIIWFLLASMGVALVSRIAKNFQDYFVNVIVQRTGAEMYSDGIKHSL
                     ELPYQVFEDQRSGETLGKLQKVRADSEKFINAFISILFQSVVGIIFVMAYAINVHWSI
                     APVFLLTVPVLAVVSNMLSKKIKVISKKILGETTALAGATTESLRNIELVKSLGLANQ
                     EINRLNTVTDKILNLELKKVKFIRSLSFIQGTTVNFLRTLFVFLLYYLIFKDEISPGK
                     MMTLMFYSFFIFGPLQELGNVIAVYREAQASLNNFSEIMEMPLEYKPQSPLQLNAIET
                     FEFSSVSFKHISAHGNAVNNISFKARRGETIAFVGPSGSGKSTLVKLLVGLYHPAEGK
                     ILYNGIDANNINLNELREQIGFVTQDTQLFAGTIRENLLFVNPHAHDDQVLNALNMAS
                     CHSLLARAENGLNTVIGEGGVKVSGGEKQRLSIARALLRNPNLLVLDEATSALDSITE
                     EEITETIKSINKNKNLITILIAHRLSTIMHADHIFVLEKGHKIEEGRHETLLEEKGLY
                     YAMWRQQIGERKKGERVVTA"
     gene            2703..3347
                     /locus_tag="PK134_01255"
     CDS             2703..3347
                     /locus_tag="PK134_01255"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRB37512.1"
                     /translation="MNAKKEKELSPLDNITDFEVFISGRLKDLPGRNSHLKLASVNRP
                     VYEPEGNYRIGSVMLLVNNKNNIPHLVMIQRAEDGKVHSGQIAFPGGKAEPDESLTLT
                     ATRELEEEIGIADKSINIIGQLSRIYIPPSNYMVYPFIATQIQTSVFLANQNEVKEIF
                     EIPLHSLIKNKTIIQGLQHQTTRGLITAPAFRIGNLVIWGATAMILSEFIDLID"
     gene            3413..4993
                     /locus_tag="PK134_01260"
     CDS             3413..4993
                     /locus_tag="PK134_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37513.1"
                     /translation="MRKHLQKLAVLGMFSMFAYTAQAQRFLTEVFPSVNVTPNVVYGQ
                     NYEVLTGTPVLKDLKMDIYEPAGAADPMTERPLIIYLHTGSYIPIIVNGNATGSRNDS
                     AAVEICKQFARRGYVVANMDYRLGWDPTNTNPNNGLDIRRGTLLNAVYRSIQDAKSCV
                     RYFRKDAATNNNVYKIDVNNFYLVGQGTGGYIALNYAVLNSPSEITLPKFISSTDAPT
                     YGIYAGQPYVNQTALGDFDGIGGVAPYNNENNPGYSNAVQFVVNLGGAMGDSSWIAAG
                     DAPMIGIHCVDDPFAPYGVGIVYVPTTPPQAVVDVIGSGEIIKWANQLGNNACFANAG
                     FTDPYTLRANSINNGNEGLFPLQQGINPVNPQLGHAGPWEWYDLQSTRNLAMAMGYQS
                     TRGDTCYGNSMLTNPTMSKTYALAYIDTIMNYVNPRIVFCRNLPTSVKEVSTNSSDVT
                     IYNSPSKSYFTVVSTSKEPMRSIEVYDIKGSLLASEKNPNAFNQRIEKASLSLNKGVV
                     LVKITYNSDVITKKITLD"
     gene            complement(5046..5951)
                     /locus_tag="PK134_01265"
     CDS             complement(5046..5951)
                     /locus_tag="PK134_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRB37514.1"
                     /translation="MNSPIIDEVNLNDFQLAHKGVSLHVLRLDLDDVITGGNKSFKLK
                     YNLQAAKEKGRSIIVTFGGAFSNHIAATARTCKLQNLKAVGIIRGEIVTPMNNTLSRA
                     VNDGMTLIPVSRQWYRQIHDKEFYKSNLVKCLLIANNINPDACYVVPEGGNNAEGFKG
                     CLEIMNNINADYDTVVCAVGTGTTLAGIAASLPQNKNVHGIAVVLAADTLTKNIQLHL
                     AESNSNAKYIVHQQYSFGGFGKSNAELKTFCELFTQKTNILIEPVYTGKLFYAILDML
                     AKDKFNPGTKILAIHTGGCQYLIDK"
     gene            6079..6255
                     /locus_tag="PK134_01270"
     CDS             6079..6255
                     /locus_tag="PK134_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRB37515.1"
                     /translation="MAFTDKFEKDEYYLTPEGYIVFTEKYHLKRGYCCQSGCRHCPWN
                     YNTKKADKNNKQKS"
     gene            6325..7965
                     /locus_tag="PK134_01275"
     CDS             6325..7965
                     /locus_tag="PK134_01275"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRB37516.1"
                     /translation="MAEKVNLEFNINEDAYKSHLIRLAERLNKIYEGGGKKNAAKQKE
                     KNKMLARERIAYLLDTDKPQFEVGAFAADGMYAEQGGCPSAGVVIVIGYISGKQCIVV
                     ANDATVKAGAWFPMTAKKNLRAQEISMENKLPIVYLVDSAGVFLPMQDEIFPDKEHFG
                     RIFRNNAVMSSMGILQIAAIMGSCVAGGAYLPIMSDEALIVDKTGSVFLAGSYLVKSA
                     IGEDIDNETLGGATTHCEISGVTDNKFPDDASCLDYIKRLFSKIGTFDKAGFERAESK
                     EPAANPKEIYGIIPAENSKQYDMHEVINRLVDNSEFDEYKEKYGQSILCGTARIDGWA
                     VGIVANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQRRIPLVFLQDVTGFMVG
                     SRSEHGGIIKDGAKLVNAMANSVVPKFTIIIGNSYGAGNYAMCGKAYDPRLIAAWPTG
                     QIAVMGGSQAAKTLLQIQVAALKAQGKEISEEDEKAQLKTITDRYNSQTSPYYAASRL
                     WVDAIIDPLETRNMISMGIEMANHNPITKPYNVGVIQT"
     gene            complement(7996..8907)
                     /gene="moaCB"
                     /locus_tag="PK134_01280"
     CDS             complement(7996..8907)
                     /gene="moaCB"
                     /locus_tag="PK134_01280"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HRB37517.1"
                     /translation="MINITHKNSTHRTAIAQAIVKVSKEETITAVKENKVPKGNVFEM
                     SKTAGLFAAKRTSDMIPDCHPLPIEYAHITHEINGLEILIRVEIQTIYKTGVEVEAMH
                     AASVVALTIYDMLKPIDKNISIEQIKLIEKKGGKSDFIKKEISGIKAAVIVCSDSISK
                     GTKEDKAGKAIIEKLKSCKVDIADYIIIPDEKNIIQEKVRAYCSQQIQLVILTGGTGL
                     SPRDITPEALDSVIEKRIPGIEEAIRTYGQERTPYSMLSRSIAGLVGNTLVLALPGST
                     RGAAESMEAVFPHILHIFRVMKDARHD"
     gene            complement(8914..9354)
                     /locus_tag="PK134_01285"
     CDS             complement(8914..9354)
                     /locus_tag="PK134_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRB37518.1"
                     /translation="MERKPKQIFIQGAISSSKIADSIQKHSSKTDIGAHSIFLGQVRT
                     DEKPEGIIQAIEYTTYEEMALQKAFEIREDIFAKYPLTCLHIYHSLGVVKTGEICLFV
                     FTSSKNRKAATEACNEMVERIKNELPVWGKEIIDEKKSLWKENS"
     gene            complement(9360..10457)
                     /locus_tag="PK134_01290"
     CDS             complement(9360..10457)
                     /locus_tag="PK134_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRB37519.1"
                     /translation="MSRFERQINLSGFGPKAQQQLQKAKVLVIGAGGLGCPVLLYLDA
                     AGVGTLAICDGDVVAASNLNRQVIFSESDITKSKAEVAGIYLKQKYSDINIEIINKFL
                     TVENTLQIFNAYDLIIDGSDNFQTRYMVNDACVLLNKPLVFGAVYQNEGQVAVFNVSD
                     DNAVSSNYRDVFPNPPSENEILNCNETGVLGVLPGIIGVMMASEAIKLITGFGKALIN
                     KMLYYNLLTNTTYETEIAPNAVAKSIIPKTPEDFYKTDYTLLCGNLPSIEWSEALLLK
                     NNTPGKVILLDVRETHEQPKLDGIETMNIPLQSLSENIEPLNTAENILVFCQSGIRSR
                     KAVAVLRSKFLQKNIYSVAGGISKLNSTINA"
     gene            complement(10468..10710)
                     /locus_tag="PK134_01295"
     CDS             complement(10468..10710)
                     /locus_tag="PK134_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB37520.1"
                     /translation="MELKGIKILAFGMIAEKINASEFYLKEVSNTNELLSSLNSLYPQ
                     LKEMKFTISVNRRIVNNNEPLNADSEVALLPPFSGG"
     gene            complement(10701..11288)
                     /locus_tag="PK134_01300"
     CDS             complement(10701..11288)
                     /locus_tag="PK134_01300"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRB37521.1"
                     /translation="MISDKSIEVFVLAGGKSSRMGEEKGLMLLHNKPMIQYVLEQLSI
                     LKLPVQIIANADDYTKFGYDTLKDIIAEKGPMGGLLTAFHYAKSDCVLLISCDMPFIT
                     SEIVSKLIFEVKDDEITAAYVQNEPNPLFAVYKKSLKNEVEKRIAAGNLQMKHLIKSF
                     RHKRVEMDVYLNRNPNLFINLNSKQDVNNITDVWN"
     gene            complement(11296..12228)
                     /locus_tag="PK134_01305"
     CDS             complement(11296..12228)
                     /locus_tag="PK134_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRB37522.1"
                     /translation="MSDNKKYLKPVSVHEAITMSKVCGDDFRYIAGATDVMVNRRQGN
                     EESFCLIDLCGIDELKQVTVKENYLSIGSLVRLDDLKKHAAIVKEFPVLIEAAETVGS
                     PLIRKSATLGGNVLCENRCIYYNQSEWWRDAVGYCLKCEGNICIATGSKKLCYSEFVS
                     DTAPALISMDAEIEIADHEGERTLKLEDIYTGDGVCPRNLKKSAVIKSILLPLQREFH
                     SSFHKLRQRQSLDFTSLTVAVSIDKNGKLKSVLGGVDPKPVVVEGNSDSNLEEIIKLA
                     LKGSRAIDNEMFSRNYRRSMISVFLKRSFEKLIG"
     gene            complement(12237..15032)
                     /locus_tag="PK134_01310"
     CDS             complement(12237..15032)
                     /locus_tag="PK134_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB37523.1"
                     /translation="MRSIKIKVNGRQHEVLIDEHETLAHVLREKIELTGTKIGCEQGS
                     CGACTVLLNDEPVLSCITPAMRCDELSVTTIEGVSENGELHLLQQKFVEKGAIQCGFC
                     TPGMVMTSLDFVRKNPHATVDEIKEALSGNLCRCTGYKKIIEAVAETIRPQQHPAEAN
                     GSKPHNGRIVGTGMPYIEAAKKVQGKAEYADDIRLKNVLHCSFLRSPYPHAKIEQIDT
                     SAAAKLEGVKYILTGVELPEKFGVLPISQDETALAIDKVRYIGEVVAAVAAETEAIAK
                     AACNLIQVKYKPLKPFFEIDESLNDIGTEEKIHEHVKFNNNVHKKAELRFGNQEEGFQ
                     SAAFKSKMNFEFEGITHGFTEPHAATAYWDENGLTIITATQVPHYLHRALSKVLKLPL
                     SRVRVIKPYVGGGFGGKSDPFPHEIIVSYLAIKTGRPVKVRFSREEVFISNHGRHPTK
                     MTMEIASDETGNFRALDADIAIDGGAYGSFGVVTTYYNGVLLQAPYKVENFGFKTFRV
                     YTNKPQCGAMRGHGAVNSRFAIETLIDDLAHQLKMDPCELRLKNFMPSNTLTAGQYRI
                     TSNGSRESLLKVMEQSQWKERYGKLPFGHGLGVGCGFFISGSALPIIRNELPQSVVHL
                     KVDFDGRVLVTSGASDIGQGSDTMLAMIVAEVLGISMDKIFVLAADTLLTPVDLGSYS
                     SRVTFMAGNAAKMAAENLKKEIINSVVARKEISADELNLADDCVFSNDRKIELTWNEA
                     IDFTIAHRGAINVSGKYISPKLGGDFKGSGAGLSPSYSFGAVVTEVKVDPETGAVKVL
                     NIWGAHDCGKAINPLAVEGQLEGSWHMGLGQAMSEQVKYFNGLLINSNFLDYKIPTSL
                     DTPPIHTNIIETADPEGPFGAKECGEGAIHPIIPAVANAVFDAVGARITKLPISPEDV
                     LQKIKNLSLPNAAQVNV"
     gene            complement(15060..15797)
                     /gene="fabG"
                     /locus_tag="PK134_01315"
     CDS             complement(15060..15797)
                     /gene="fabG"
                     /locus_tag="PK134_01315"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRB37524.1"
                     /translation="MPDRIAIITGAGKGIGKAIAERLSADGFYVALVDVDQAAMEEAV
                     AIIGADKATGYLVDITNENQVKALFENIENAKNKIDLLVNNAGIIRDNLIWNLSANDF
                     DAVINVNLKGTWLMCREAAIRMKQQKYGCIVNIASRAWLGNAGQTNYSASKAGIIGLT
                     RSLALEMGRHNVSVNAIAPGLIDTPLTQKLDKDVLDKLIQAQPSRSMGKPEDIANVVS
                     FLASEKTSFMTGQTIYVDGGKSIGAGL"
     gene            complement(15760..16596)
                     /locus_tag="PK134_01320"
     CDS             complement(15760..16596)
                     /locus_tag="PK134_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRB37525.1"
                     /translation="MRSIYEIIAGLEQCGEEAALCIITETRGSTPLKAGAKMIVHRNK
                     NISGTIGGGNLEKQVIEDAVSVMESGKAATFEHQLVRDHQMCCGGSVFVFIEPVKKRN
                     KLIVFGAGHVGKEVAKLACSLNFKISLVDERKEMLTGLALDDAKIYNMHHLQFLSDVV
                     LDENTFVVICTHLHHYDREILARCIKQPYAYLGMIGSMRKVVITRKKFISEGLASEEE
                     LSKVDMPMGFDIGGNSPAEIAISIVAKLVAVMNGHTMQNNNEEKSNYTYAGQDSDNNR
                     CW"
     gene            complement(16575..17186)
                     /locus_tag="PK134_01325"
     CDS             complement(16575..17186)
                     /locus_tag="PK134_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRB37526.1"
                     /translation="MKSSVSAIILAAGQSKRMNRPKPFLAFNEMENFIEKIGSTYLAA
                     GIEKAIVIVNPDIEREVSVLLKNKFSDSKLEVVVNSYPEKGRFYSIQLGLEKTDTDSC
                     FIQNTDNPFVTKDLLNNMCRMIKQGAYVVPVFKNEKGHPVIISAEVMNHCRKLNGNDF
                     NLRDELQMFEEIRLVWDEKNILANINTEDEYQKYFPSCEAFTK"
BASE COUNT         5093 a         3438 c         3249 g         5523 t
ORIGIN      
        1 attagagata ttattgtgag tgacttctac atcgggatta gcttcagtga taaaatattc
       61 cctatatcta attgtttgag ctgctataaa agctatataa ataattaagc acgcaattat
      121 ttcaaataac aattgtcgtt ctgtgtaata tgggccaata atactacttg taaaggtggg
      181 taatatcatt gtgaatgtta caatagacac aagtgctacc agtgcaatct ttactgaatg
      241 aggagaaaat gtttgtgtat gatattttct tccgccaaga aacaatgaaa gtcctataat
      301 tccattaaga atcaacataa cagcagcata aacagtgtct ctggcgaatg aagcatattc
      361 tttaccaccg gtcatcatta aagaaattat tatagcaact tcaataattg taatggatac
      421 tgccaggata atagttccgt aaggttcgcc cacacgatga gcaacgattt ctgaatgatg
      481 tacagctgac attacacaaa acagcagtaa tattcctgcc aatatttgca aaacaatatt
      541 gttatctaat aaacctgaaa aatatagtaa acatgtaata ataggtaagg aggttgtcca
      601 ttcatatagt ttaacgattt tttgcatata aaataaactg tgtcttttaa ttattttcta
      661 tgtatcaatt agctgttgca tagaattgtc aatgagatag taagtccaat cgctatcgag
      721 catattgttt tgttctgata catatcagaa ttaaaactat ttttcaattc aaaaataaaa
      781 ataaaaagat aaaataagcc agtaatgaat ttttattttt aaaagaatta taagttgact
      841 ttttaaaatt gacacatttt tataataaaa ccttacctaa atattaaccc tcccactcaa
      901 tattaataca aattaaacta ctgattcatt tcctacttcc taaaccacta tttttgaaac
      961 tcctaacaaa tcaagaagtt tgaaaatatt agtaacttat ttaaaacgcc actggaagtt
     1021 ggtattatta gctttgttgc tggcggctgt taatcaatgt ttttcgttga ttgcgggtaa
     1081 aatgctggat cgatttgcta tgcatgtatc gaactacagt aatgattttc ctaaattttt
     1141 tcaggatatt atttggttcc ttttagcttc aatgggagta gctttggtgt cgcgtattgc
     1201 aaaaaatttt caggattatt ttgtaaatgt gattgtgcaa cgcaccggtg cagagatgta
     1261 tagcgatggc attaaacatt cgctggaatt gccctatcag gtttttgagg atcagcgcag
     1321 cggtgaaacc ctaggtaagt tacaaaaggt gcgtgccgac agcgagaagt ttatcaatgc
     1381 atttatcagt attctttttc agagtgtggt tggaataatt tttgtgatgg cttatgctat
     1441 aaatgtacat tggagcattg cacctgtttt tctgcttact gtgccggttt tggctgtggt
     1501 gagcaatatg ctcagcaaaa agattaaggt tatttctaaa aaaatattgg gtgagactac
     1561 tgcattagca ggtgcaacaa cagagtcgct cagaaatatt gagttagtga agagtctggg
     1621 gctggcaaat caggaaataa acagactgaa cacagtgaca gataaaattc tgaatctgga
     1681 attgaagaaa gtgaagttta tccgcagcct tagttttata cagggcacaa cggtgaattt
     1741 tctgagaaca ttgtttgttt ttttgttgta ctatctgatt tttaaggatg aaatttcacc
     1801 tggtaagatg atgacattaa tgttttattc gttttttatt ttcggcccgc tacaggagtt
     1861 gggtaacgtt attgctgttt accgcgaagc acaggcttca ctgaataatt ttagtgagat
     1921 aatggaaatg ccacttgaat acaaaccaca atcacctctt cagttgaatg caattgaaac
     1981 atttgaattt agtagtgttt cttttaaaca tatatccgca catggcaatg ctgttaacaa
     2041 tatcagtttt aaagccagac gtggcgagac aattgccttt gttggtcctt cgggcagtgg
     2101 taaaagtact ttagtaaaat tactagtggg cttgtatcat ccggcagagg gaaaaattct
     2161 ttacaatggg attgatgcca acaacattaa tctgaatgag ttgagagagc agattggttt
     2221 tgtgacacag gatactcaat tgtttgcagg aactatcaga gaaaatcttt tattcgtaaa
     2281 tccacatgcc catgatgatc aagtattgaa tgcattgaat atggcatcgt gccactctct
     2341 tttggcaaga gcagaaaatg gcctgaacac agttattggc gaaggcggtg tgaaagtgtc
     2401 aggaggtgaa aagcaaagac tttctattgc aagagctttg ctgcgcaatc ctaatctgtt
     2461 ggttttggat gaggctactt ctgcacttga ttccattact gaagaagaga ttacagaaac
     2521 tataaaatct atcaacaaaa ataaaaatct cattaccata ttgattgcac acaggctttc
     2581 aaccatcatg catgctgacc atatttttgt attggaaaaa ggacataaga ttgaagaagg
     2641 cagacatgaa acgcttctcg aagagaaagg tctttattat gccatgtggc gtcagcagat
     2701 tggtgaacgc aaaaaaggag aaagagttgt caccgcttga taacataaca gactttgaag
     2761 tttttatttc cggaagactg aaagatttgc ccggcagaaa ttctcattta aaattagcat
     2821 ctgttaaccg tcctgtttat gagcctgaag ggaattatcg cataggcagt gttatgttgc
     2881 ttgtcaataa taaaaataac attccgcatc ttgtaatgat tcagcgtgca gaagatggca
     2941 aagtgcattc gggtcaaatt gctttccctg gtggaaaggc agagcctgat gagagtttga
     3001 ctctaactgc aacccgcgaa ctggaagaag aaattggaat tgctgataag agcatcaaca
     3061 tcataggtca gctttcaaga atatatattc caccgagcaa ttacatggta tatcctttta
     3121 ttgccactca gattcaaaca tcagttttct tagcaaatca aaatgaagtg aaagaaattt
     3181 ttgaaatccc attgcatagt ttgattaaaa ataaaacaat tatacaagga ctgcaacatc
     3241 agactacaag aggcttaata acagctcctg ctttcagaat tggcaacctg gttatttggg
     3301 gtgcaacagc aatgatttta tcagaattta ttgacctgat agattaagtt ttcagtaatt
     3361 gctttttatt tgaagattgt tattattttt acgtccataa aaccatatta agatgagaaa
     3421 acatttacaa aaattagcag tattgggcat gttcagcatg tttgcatata ctgcacaggc
     3481 acaacgattt ttaactgaag tattcccttc tgttaatgtt acccccaatg tagtttatgg
     3541 tcagaactac gaagtcctta caggaacacc agttttaaaa gatttaaaaa tggatattta
     3601 tgagcctgca ggtgctgctg acccaatgac tgaaagaccg ttgattattt atttacatac
     3661 aggaagttat attcctatta ttgtgaatgg aaatgcaacc ggatcaagaa atgacagtgc
     3721 agcagtagaa atttgcaagc agtttgctcg cagaggctat gttgttgcaa atatggatta
     3781 ccgcttagga tgggatccta caaatacgaa tccaaacaat ggacttgaca ttcgtagagg
     3841 taccttgctg aatgcagttt atcgttcaat acaagatgca aagtcatgtg tgcgatattt
     3901 cagaaaagat gctgcaacaa acaataatgt ttataaaatt gatgtgaata atttttatct
     3961 cgttggtcag ggaacaggcg gatatattgc attgaactat gcagtgctaa acagtccgtc
     4021 tgaaattacg ctgcctaagt ttatcagcag cactgatgca ccaacctatg gcatttatgc
     4081 cggacagcct tatgtaaatc aaactgcatt gggtgatttt gacggcattg gtggtgtggc
     4141 accctataac aatgaaaata atcccggata ttcaaatgct gttcagtttg tagtgaattt
     4201 aggtggtgcc atgggtgata gttcatggat tgctgccggt gatgctccta tgattggaat
     4261 tcattgtgtt gacgatccat ttgctcctta cggtgttgga attgtttatg tacccactac
     4321 tccaccacaa gctgttgttg atgtaatagg ttcaggtgaa attattaaat gggctaatca
     4381 attaggaaat aatgcatgtt ttgctaatgc agggtttacg gatccttata cacttcgtgc
     4441 taacagtatt aataatggca atgaaggatt gttcccactt cagcaaggta ttaatccggt
     4501 aaacccacaa cttggacatg ccggtccttg ggaatggtac gatttacaat caacacgtaa
     4561 tcttgctatg gcaatgggtt atcagtcaac acgcggagat acttgttatg gtaattctat
     4621 gttaacaaac ccaactatga gcaagactta tgctttggct tacattgata ccattatgaa
     4681 ctacgtaaat cccagaatcg ttttctgccg taacctgcca acatctgtta aagaagtttc
     4741 tacaaacagt tcagatgtaa caatctataa tagtccttca aaaagctatt ttactgtggt
     4801 ttctaccagc aaagagccaa tgcgttctat tgaagtttat gatattaaag gcagtctttt
     4861 agcatctgag aaaaatccaa atgcatttaa tcagcgaata gaaaaggcat cactatcact
     4921 gaacaaaggt gttgtattag taaaaataac ttacaactct gacgtgatta ccaaaaaaat
     4981 aactttggat taagttttat aaagtctaat atattttgaa aaggcagtct tcggattgcc
     5041 tttttttatt tatcaatcaa atactgacaa ccaccggtgt gaatagcaag aatttttgtt
     5101 cccggattaa acttatcttt tgcaagcata tcaagtattg cataaaacaa tttaccggtg
     5161 taaacaggct caataagaat attagttttt tgtgtaaata actcacaaaa tgttttaagt
     5221 tctgcattgc ttttaccaaa tccaccaaag gagtattgtt ggtgaacaat atactttgca
     5281 tttgaattac tttcagctaa gtgtaattgt atgtttttag tcagagtatc ggcagccaga
     5341 actacggcaa taccatgtac gtttttattt tggggtagtg atgcagcaat tcctgcaaga
     5401 gtagttcctg ttccaacagc acatacaact gtatcataat cagcatttat attattcatt
     5461 atttccaaac aacctttaaa gccttcggca ttatttcctc cctcaggaac tacataacaa
     5521 gcatcaggat ttatattgtt tgcaattagt aaacacttta ctaaattact tttataaaat
     5581 tctttatcat gaatttgtct ataccattga cgactgacag gtattaacgt cattccatca
     5641 ttaacagcac gacttaaagt gttattcatt ggcgtaacaa tttcacctct gataataccc
     5701 acagctttta agttttgtaa tttgcaggta cgtgcagttg ccgcaatatg attgctgaaa
     5761 gcaccaccga atgtaactat tatgctcctc cccttttctt ttgctgcttg aagattgtat
     5821 tttaacttaa aagacttatt gcctcctgtt atgacatcat ccaaatcgag tctgagtaca
     5881 tgtaaactca ctcctttatg tgccaactga aagtcgttga gatttacttc atcaataatt
     5941 ggactattca tttttgcaaa tctaaagagt tatacatgaa ttgctttgag tatatacatc
     6001 cttaatttca tcactcaata cgatacgata aaatattatt tgaaaacaat ctgtatattg
     6061 ttgaattttg caaacgaaat ggcttttaca gacaagtttg aaaaagatga atactacctg
     6121 actcctgaag ggtatattgt atttactgag aaatatcatc taaaaagagg atattgttgt
     6181 caaagcggtt gccgccactg tccatggaat tacaatacta agaaggcaga taagaacaat
     6241 aaacaaaagt catagacttg cctaaagtac attcagtaaa gttgactttt ttaagttatt
     6301 tttgcaggct attgaatact aattatggca gagaaagtta atcttgaatt taatatcaat
     6361 gaagatgctt ataaatcaca tttgattcgt ttagcagagc gattaaataa aatttatgaa
     6421 ggtggtggaa agaaaaatgc agctaagcaa aaagaaaaaa acaaaatgct ggcacgtgaa
     6481 cgaattgcgt atttgttgga taccgataaa ccacaatttg aagtaggtgc ttttgcagca
     6541 gatggcatgt atgccgaaca aggtggttgt ccttctgcag gtgttgttat tgtgattgga
     6601 tatatttcag gaaaacaatg tatcgttgta gctaatgatg ctactgtaaa ggcaggtgca
     6661 tggtttccga tgacagcaaa aaagaattta cgtgcacagg aaatcagcat ggaaaataag
     6721 ttacctattg tttatcttgt tgacagtgca ggtgtatttc ttcctatgca agatgaaata
     6781 tttccggaca aagagcattt cggtcgcata ttcagaaaca atgcagttat gtcatcaatg
     6841 ggaatattac agattgcagc aattatggga agttgtgttg caggaggagc ctatttacct
     6901 attatgagtg atgaagcttt aatagttgac aaaacaggtt ctgtgtttct tgcaggaagc
     6961 tatttggtaa agtctgctat tggtgaagat atagataatg aaactttagg tggagctact
     7021 actcattgtg aaatttcagg tgttacagat aataaatttc ctgatgatgc atcttgtctt
     7081 gactatatta aacgtttgtt ttctaaaatt ggaacatttg acaaagcggg ttttgagaga
     7141 gcagagtcaa aggaacctgc agcaaatcca aaagaaattt atggaataat tccggcagaa
     7201 aattccaagc aatacgatat gcatgaagtt atcaaccgat tagttgataa cagtgagttt
     7261 gatgaataca aagaaaagta cggacaaagt attttatgcg gaacagcacg tattgacgga
     7321 tgggctgtag gaattgttgc caatcagcgc aaggttgtga aaagtaaaaa aggcgaaatg
     7381 cagtttggtg gtgttatcta cagcgacagt gcagataagg cagcaaggtt tatcatgaac
     7441 tgcaatcagc gaagaatacc cttagtgttt ctgcaagatg taacaggctt tatggttggt
     7501 agtcgtagtg aacatggtgg cataatcaaa gatggagcta aattggtaaa tgctatggct
     7561 aacagtgtcg tgcctaaatt caccatcatc attggtaaca gttatggtgc aggcaactat
     7621 gcaatgtgtg gcaaagccta cgatccaaga ttaattgctg catggccgac aggacaaata
     7681 gcagtgatgg gtggttcaca agcagcaaaa acattattgc aaattcaggt ggctgctctt
     7741 aaagcacagg gtaaagaaat ttctgaagaa gacgaaaaag cacaactaaa aacaattact
     7801 gacagataca actcacagac atcaccatat tatgcagcat caagactttg ggttgatgca
     7861 attattgacc cattagagac tcgtaatatg attagcatgg gcattgaaat ggcaaatcac
     7921 aatcctatta ctaaaccata taatgttggg gtaattcaga cttgattaca agagttttct
     7981 ttaaggtata ttttctcaat catgcctcgc atctttcatc actctgaaaa tatggagtat
     8041 gtgagggaaa acggcttcca tactttcggc agcaccgcgt gtggagccgg gcaaagccaa
     8101 tacaagggtg ttgcctacta atcctgcaat actgcgcgat agcatagagt atggtgttcg
     8161 ttcctgacca tacgtgcgga tggcttcttc aatgccgggt atccgttttt cgataacgct
     8221 gtctaatgct tccggagtaa tatcgcgtgg cgataatcct gtgccgcccg taagaattac
     8281 cagttgaatc tgctgagaac aatatgctct tactttttcc tgaataatat ttttttcgtc
     8341 aggaatgata atgtaatcgg caatatcaac tttacaagat tttagttttt caataattgc
     8401 tttacccgcc ttgtcttctt ttgtgccttt tgaaatactg tcggagcaaa ctataacggc
     8461 tgcctttatt ccgcttattt cctttttaat gaaatcgctt ttcccgcctt tcttttcaat
     8521 cagttttatt tgttcaatgg aaatattttt atcaataggt ttaagcatat cataaatggt
     8581 aagtgcaaca acagaagcag catgcatggc ttctacttct actccggttt tgtaaatggt
     8641 ttgaatttcc acgcggatta aaatttccaa cccgtttatc tcatgtgtga tgtgtgcata
     8701 ttcaatggga agaggatggc aatcgggaat catatcggat gtgcgtttgg cggcaaacaa
     8761 tcccgcagtt ttcgacattt caaacacatt gcctttgggc actttatttt ctttaacggc
     8821 agttatcgtt tcctctttac tcactttcac aattgcctgt gctatggcag tgcggtgcgt
     8881 gctgttttta tgcgtaatgt ttatcatact tctttaactg ttttctttcc acaaagattt
     8941 tttctcatca ataatttctt tcccccaaac gggcaattcg tttttgattc gttccaccat
     9001 ctcattgcaa gcttctgttg ctgctttccg gttttttgaa gaagtaaaaa caaacaaaca
     9061 gatttctccg gtttttacaa cacccaaact atgatagatg tgtaaacagg tgagcgggta
     9121 ttttgcaaaa atatcttcgc gtatctcaaa tgctttctgt aaagccatct cttcgtaagt
     9181 tgtgtattca atcgcctgaa tgattccttc cggcttttca tcggttctta cctgccccaa
     9241 aaaaatactg tgtgcgccaa tatctgtttt actgctgtgc ttttgaatgg agtctgcaat
     9301 ttttgaggaa gaaatggccc cctgtataaa aatctgtttt ggtttacgtt ccatgtttgc
     9361 taagcgttga tggttgaatt aagttttgaa attcctccgg caacagaata aatatttttc
     9421 tgtaggaatt tgctgcgtaa cactgcaaca gcttttctgc ttcttattcc cgactgacaa
     9481 aaaactaaaa tattttctgc tgtatttaat ggctcaatat tttcagaaag tgactgcagc
     9541 ggaatattca ttgtttctat tccatccaac ttcggctgtt cgtgtgtttc cctcacatca
     9601 agcaaaataa ctttgccggg agtattattt ttcaataaca acgcttcact ccactcaatg
     9661 gatggcaaat tgccgcatag tagggtgtaa tcagttttgt aaaagtcttc tggggttttt
     9721 ggaataatac tttttgcaac agcgttgggt gcaatttctg tttcgtaagt ggtgttggta
     9781 agcaggttgt agtaaagcat tttatttatc aaagctttgc caaatcccgt tattaatttt
     9841 atcgcttccg aagccatcat tacaccaata atgccgggca atacacccaa aacaccagtt
     9901 tcattgcagt ttagaatttc gttttcagaa ggcggattcg gaaatacatc acggtaattt
     9961 gaagagacgg cattatcatc acttacatta aacactgcca cctgaccttc gttttgatac
    10021 actgccccaa acacaagtgg tttattcagc agcacacagg catcgttaac catatagcgc
    10081 gtttgaaaat tatcgctacc atcaattatc aaatcataag cgttgaaaat ttgaagcgta
    10141 ttttcaacag ttaaaaattt attgataatc tcgatattaa tatcgctgta tttttgcttc
    10201 aggtaaatcc ccgcaacttc tgcttttgat ttagtgatat ccgattccga aaaaattacc
    10261 tgacggttaa ggttagaagc ggcaaccaca tcaccgtcac aaatggctaa tgtgccaact
    10321 cctgctgcgt caagatacaa cagcacagga catcccaatc cgcctgctcc tatcaccagc
    10381 acttttgctt tttgcagttg ttgctgtgcc tttggcccga aaccggataa atttatttgc
    10441 ctctcaaacc tgctcattat ggtttaatta tcctcctgaa aatggcggaa gcaatgccac
    10501 ttcgctgtcc gcatttaaag gttcgttgtt atttacaatt cttctgttta ctgaaattgt
    10561 aaacttcatt tctttaagtt gagggtataa actgttcagc gaactcagca gttcattggt
    10621 attgctcact tctttcaaat aaaattcgct cgcgtttatt ttttcagcaa tcattccaaa
    10681 cgctaaaatc ttaattcctt tcagttccat acatctgtaa tattatttac atcctgctta
    10741 ctattcagat tgataaacaa gttaggattc cgattcaaat atacatccat ttccaccctt
    10801 ttgtggcgga acgattttat gagatgcttc atttgtaagt ttcctgctgc tattcgtttc
    10861 tccacttcgt ttttcagtga ttttttatag actgcaaaca atggattggg ttcgttttga
    10921 acataagccg cagtgatttc atcatccttc acctcaaaaa ttagtttgct gactatttct
    10981 gatgtgatga aaggcatatc gcaactgata agcaaaacac aatctgattt ggcataatga
    11041 aaagctgtaa gcaagccgcc catcggtcct ttttcagcaa taatatcttt cagcgtatca
    11101 tatccaaatt tggtgtaatc atcagcattg gcgattattt gcactggtaa tttgagaatg
    11161 gatagttgtt ccagcacata ttgtatcata ggcttgttat ggagcaacat cagtcctttt
    11221 tcttcaccca tgcggctgct cttgcctccg gctaaaacaa atacttcaat gcttttatca
    11281 gaaatcataa tcgcgtcatc ctatcaactt ctcaaaactg cgtttcagaa aaacactgat
    11341 catggatctg cggtaattgc gtgagaacat ttcgttgtca atggctcggg agccttttaa
    11401 tgccagtttt attatttctt caagattact atcactgttg ccttccacca caaccggttt
    11461 gggatctact ccaccgagaa cggattttag ttttccgttt ttatctatgg aaacggcaac
    11521 cgtaagcgaa gtaaaatcca ggctttgcct ttgcctcagt ttgtgaaatg aagaatgaaa
    11581 ctcacgttgc aagggcaaca atatagattt gatgacagcc gattttttaa ggttgcgtgg
    11641 gcaaacgcca tcaccagtat aaatgtcttc aagtttcagg gtgcgttctc cttcatggtc
    11701 ggctatttca atttctgcat ccatgctgat taatgcaggc gcagtatcgg aaacaaattc
    11761 ggagtagcaa agttttttgc tgcctgttgc aatacagata ttgccttcgc atttgaggca
    11821 ataacctaca gcatcacgcc accactctga ttgattgtaa taaatacaac gattctcaca
    11881 taaaacattt ccgcctaatg tggctgattt cctgatgaga ggcgaaccca cagtttcggc
    11941 tgcttctatc aacacaggaa attcttttac gatggctgca tgtttcttta aatcgtctaa
    12001 cctcaccagc gaaccgattg acagataatt ttcttttacc gttacctgct tcagttcatc
    12061 aatgccgcac aaatcaatca ggcaaaaaga ttcttcattt ccctgcctgc ggttaaccat
    12121 cacatccgtt gcgcctgcaa tgtatcggaa atcatctcca cataccttgc tcatggttat
    12181 agcttcgtga acagaaaccg gctttaaata ttttttgttg tcactcattt ttattcttac
    12241 acatttacct gtgctgcatt tggaagtgaa agatttttaa ttttctgtaa aacatcttca
    12301 gggctgatgg gcaactttgt aatccttgca cccactgcat cgaacactgc atttgcaaca
    12361 gcaggaatta tcgggtgaat ggctccttcg ccacattctt ttgcgccaaa tggcccttcg
    12421 ggatctgctg tttcaatgat gttggtgtgg ataggcggtg tatcgagcga agtgggaatt
    12481 ttataatcta agaaattact gttgatcagt aagccgttga aatattttac ctgctcgctc
    12541 attgcctgtc ccaatcccat gtgccacgag ccttccaatt gtccttccac tgccaatgga
    12601 ttaattgctt ttccgcaatc gtgtgcgccc caaatattca acactttcac ggcaccggtt
    12661 tccggatcaa ctttaacttc tgtaactacc gctccgaagc tatacgaagg actcaatcct
    12721 gcacccgagc ctttaaagtc gccaccgagt tttggagaaa tatattttcc gctaacgttg
    12781 atagcgccac gatgtgcaat ggtaaaatca attgcttcgt tccatgtcag ttcaattttt
    12841 ctatcgtttg aaaacacaca atcatcagca aggttcaatt catctgctga aatttctttt
    12901 cgtgcaacca ctgagttgat aatttctttt ttaagatttt ccgctgccat cttcgctgca
    12961 ttgcccgcca taaaagttac acggcttgaa taactgccga ggtcaacagg tgtaagcaag
    13021 gtatctgccg caagcacaaa aattttatcc atcgaaatgc ccagcacttc ggcaacaatc
    13081 atggcaagca tggtatcgct tccctggccg atgtcgcttg caccggaagt aaccaacacc
    13141 cgtccatcga aatcaacttt taaatgcacc accgattgcg gcaattcatt cctgataatg
    13201 ggtaatgcgc tgccgctgat aaaaaatcca cagcccactc caagtccatg accgaaaggt
    13261 agtttaccat atctctcttt ccactgcgat tgctccatca ctttaagcaa cgattcacga
    13321 ctgccgtttg aagtgatgcg atattgacct gccgtgagtg tattggatgg catgaaattt
    13381 tttaatcgca actcacacgg atccatcttt aattgatgcg ctaaatcatc aatcagcgtt
    13441 tcaatggcaa agcgtgaatt cacagcgcca tgtccacgca tggcaccgca ttgcggctta
    13501 ttggtgtaaa cacgaaaagt tttgaagccg aaattttcaa ctttataagg cgcctgcaac
    13561 aacacaccat tgtaataagt agtaaccaca ccaaaacttc cataagcacc gccatcaata
    13621 gcaatatcag catcgagagc acggaaattt cctgtttcat ccgaagcaat ttccatcgtc
    13681 attttagtgg gatgccgtcc gtgattgctg ataaaaactt cttcacggct gaaacgcact
    13741 ttaaccggtc gcccggtttt tatggcgagg taagacacaa ttatttcatg cggaaaagga
    13801 tcgctcttgc cgccaaatcc accgccaaca taaggtttaa taacacgaac cctgctcaaa
    13861 ggtaacttca atactttgct caaagccctg tggagatagt gaggcacctg tgtggcagta
    13921 atgatggtca aaccgttttc gtcccaatat gcagtggcgg catgtggctc agtgaaacca
    13981 tgagtgatac cttcaaattc aaaattcatt ttgcttttaa atgctgcact ttgaaatcct
    14041 tcttcctggt ttccaaaacg caactctgct tttttgtgaa cattgttatt gaacttcaca
    14101 tgctcatgga ttttttcttc cgtgccgatg tcgtttagtg attcatcaat ttcaaaaaac
    14161 ggtttcagtg gtttgtattt tacctgaatg aggttgcagg cagcttttgc aatggcttct
    14221 gtttcggcag ccactgcagc caccacttcg ccaatgtatc ttaccttatc aattgccaac
    14281 gctgtttcat cctgcgaaat gggcagcact ccgaattttt ccggcagttc aactccggtg
    14341 agaatatact ttacaccttc cagttttgct gcagcacttg tgtcaatctg ctcaatcttt
    14401 gcatgtggat aaggacttct taaaaaactg caatgcaata catttttcaa acgaatgtca
    14461 tcggcatatt ccgctttgcc ctgcactttt ttagctgctt caatgtaagg cattcccgtt
    14521 ccaacgattc tgccgttgtg cggcttgcta ccatttgctt cagccggatg ctgttgcgga
    14581 cggatggttt cagcaacggc ttcaattatt tttttgtaac ccgtgcagcg gcaaagattt
    14641 cccgataatg cttctttgat ttcatcaacg gtcgcatggg gatttttcct tacaaagtcc
    14701 aatgaggtca tcaccattcc cggagtgcag aagccgcatt gaatggcgcc tttctctaca
    14761 aacttttgtt gaagcaaatg caattcacca ttttcagaaa cgccttcaat ggtggtaaca
    14821 gacaactcat cacaacgcat ggctggtgta atacaactga gcaccggttc atcattcagc
    14881 agaacagtgc aggctccgca acttccctgt tcgcagccaa tctttgtgcc tgtcaattca
    14941 attttttccc gcagcacatg agcaagggtt tcatgttcat caatcaacac ttcatgctgc
    15001 cttccgttta cctttatttt aattgacctc attattcaat atttattact gcaattattt
    15061 tataacccag cccctatact ttttccgcca tcaacataaa tggtttgccc ggtcatgaaa
    15121 cttgtttttt ctgaagcaag aaatgaaacc acattagcaa tatcttcagg cttgcccatg
    15181 cttcgtgagg gttgagcctg aatcagttta tccaaaacat ctttatctaa cttttgggta
    15241 agtggtgtgt caattaaacc gggagcaatg gcattaactg aaacattatg tcttcccatt
    15301 tccaatgcca aggaacgtgt aagcccgatg ataccggctt ttgaagcgga gtagttggtt
    15361 tgtcccgcat taccaagcca tgcacgggaa gcaatgttca caatacagcc atacttctgt
    15421 tgcttcattc gtatcgcagc ttcacggcac atcagccatg ttccttttaa gttgacattg
    15481 atcactgcat caaaatcatt tgccgataaa ttccagatta aattatcacg gataattccg
    15541 gcattgttca ccaacaaatc aattttattt tttgcgtttt caatattttc aaataatgct
    15601 tttacctggt tttcattggt gatatcaaca aggtaaccgg tagctttatc agcgccaatg
    15661 attgcaaccg cttcttccat ggctgcctgg tcaacatcaa ccaatgccac ataaaagccg
    15721 tctgctgaca accgttcggc aatggctttg ccaattccct taccagcacc tgttattatc
    15781 gctatcctgt ccggcatacg tataatttga tttttcttcg ttgttatttt gcatagtgtg
    15841 cccgttcatt acggcaacca attttgcaac aatgctaatg gcaatttcag caggcgaatt
    15901 tccgccaatg tcaaaaccca ttggcatatc tacttttgaa agttcttctt cacttgccaa
    15961 tccttcgctt ataaatttct ttcttgtgat aactactttt ctcatgctgc caatcatgcc
    16021 taagtaagca taaggttgtt tgatgcagcg agccagaatt tccctgtcgt aatgatgcaa
    16081 gtgagtacag atgactacga atgtattttc atccaaaact acatcagaca aaaactgcag
    16141 atgatgcata ttgtaaattt ttgcatcatc caatgccagt ccggtgagca tttcttttct
    16201 ttcatcaaca aggctgattt taaaattcag tgagcaggca agtttcgcaa cttctttacc
    16261 tacatgacca gcaccgaata caatcagttt gtttctcttc ttaaccggct caatgaaaac
    16321 aaacaccgaa ccgccacaac acatctgatg gtcacggact aattgatgtt caaaagtagc
    16381 agccttgcca ctttccatta cggacacagc atcttctatc acctgctttt cgaggttgcc
    16441 gccaccgatg gtgccggaaa tatttttatt tcggtgcaca atcattttgg cgccagcttt
    16501 aagcggagta gatccacggg tttctgtgat gatacagaga gccgcttctt cgccacactg
    16561 ttcaagaccg gcaattattt cgtaaatgct tcgcatgaag gaaaatattt ttgatactcg
    16621 tcttccgtat taatattagc taaaatattt ttttcgtccc aaactaacct tatttcctca
    16681 aacatttgca gttcatcacg caggttaaaa tcatttccgt tcaatttcct gcaatggttc
    16741 attacctccg cactaatgat aacaggatga cctttttcat tcttaaacac gggaactaca
    16801 taagcaccct gcttaatcat tcggcacata ttattcagca aatctttggt tacaaaagga
    16861 ttatcagtgt tttgtataaa acaagaatcc gtatccgttt tttccaatcc gagttgtatg
    16921 gaataaaatc ttcctttctc cggatatgaa tttacaacaa cttcaagctt gctgtctgaa
    16981 aatttatttt tcaataatac actgacttct ctttcaatat cagggtttac aattacaatt
    17041 gctttttcaa tacctgctgc aaggtatgtg cttccaattt tttcaatgaa attttccatt
    17101 tcattaaaag caagaaaagg ttttggtctg ttcatccgct tcgattgacc tgcagccagt
    17161 attattgctg aaacggagct tttcattttc cttcgtattg aattttttta ccgatagaag
    17221 tcaatcccag caaagcatcg ctggttgaaa aaatttcgga cagatgttcc agttcagaag
    17281 cacatccctt tttctcttct att
//
LOCUS       DAORQT010000016        17166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_288_length_17166_cov_8.467126, whole genome shotgun sequence.
ACCESSION   DAORQT010000016 DAORQT010000000
VERSION     DAORQT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_17166_cov_8.467126"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2048
                     /locus_tag="PK134_01330"
     CDS             <1..>2048
                     /locus_tag="PK134_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRB37527.1"
                     /translation="QHKTIKWFKLPVINPSIVKYAIIAILLITVYFYFEASNIRFNSD
                     VETLSFHPTELKNKEAELTGINPSIEKKIYLIASAKNYNSAVDANYKAGTLIKNLITH
                     GIIKNTLSTADYFIPDSLCKQRQLRWNYFWQQNKTRVTNQIKLSADEAGFSNNAFAPF
                     QEMIDDSIYQPLSKDLLKTMGLDLMFDSSAKESVFISSFTVATSQKDSVKALFKDIPH
                     LTVFDRSEMASLLLESVRSDFNYLLYLTAGMVFFTLLLIYGRVELALLAFLPMVVSWI
                     WILGMSSTFNIEFNFVNIVITTFIFGLGDDFSIFVTDGLLNRYKFRKDNLKSYQAAII
                     LSALTVIVGTGVLYFAKHPAIHSIAALSVIGITCILLISFLVQPFIFKLFVERRIKNG
                     HNPISFLQLFMSVFCFTYFIVGCLTFYPVLLLISLFPAPVKIKRKAVNFIVSKFSASV
                     LYSGIHVKKKFYNLDQLDFKKPSIIIANHSSFLDIMIMLMLNRKVIIMAKDWVYRSPL
                     FGLVVRYVGYIFSEDGTEDNITNIKAKMDEGYSIMIFPEGSRSSDGNLNRFHKGAFYL
                     AEKLNADIQPVMIHGAGDVSPKNEMMVKDGTINVKALPRIFANDLSWGNDYRSRQKSI
                     SQYFKEQYRLFKDEREDADYLWQKVYYNYIFKGPVLEWYGRIKWNLEKKNYDFYNNLI
                     G"
     assembly_gap    2049..2089
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2090..2571
                     /locus_tag="PK134_01335"
     CDS             <2090..2571
                     /locus_tag="PK134_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRB37528.1"
                     /translation="FMDLFLHYKNRERKISAFDYDDDKIAIAANVWNKNANLQFFAAD
                     ITKTEVPSSEVIFLNDVLHYLNIDSQEKVLQKCADALMPGGIMFIRDGVADDIKHRNT
                     KFTELLSTKFFSFNKKMNDLNFFKTEYIYQFAKRNNLKIEEHKHSNNTSNRLFVLSK"
     gene            2568..4115
                     /locus_tag="PK134_01340"
     CDS             2568..4115
                     /locus_tag="PK134_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB37529.1"
                     /translation="MKINDKIVYDIAIIGGGFSGMCCAYILAKEGYKVALVEKNIQLG
                     GSLQIFSRDKKILDTGLHYIGGYDEGENLYQLFSYFGLKDTLQTKRMDDNGYDYVSFS
                     DGRKYAHAMGYDNFINSLCKDFPDEEKAIHRFCDKLKEYCQKFPLYDLSNKITDDYFN
                     EELRNENAWDYIASITENKTLRNVLAGNNMLYAGIKEKTPMFVHALIFNSYICGSYKI
                     VTGGAQLIKQFASNIRKAGADIYTHKQITGATYKENREISQLLLENGECITALKYISA
                     IHPNETISLLGNDRFSHAWVNRVKQMDQTVSAFLVQITFKPKSFPYMNYNMYHFFNDD
                     VWDTINYTEDNWPLSFFVFTPVPLKDPQYAESISVMCYMKSDDVAQWKDSFNTTTHPS
                     NRSESYQEFKRRKEEKVLEKLETLFPEIRKAILNVYSSTPLTFKDYTASTDGSIYGVL
                     KDSNAPLKSMLQPRTKIPNLWLTGQNIVFHGILGTAISALLTCFEFTDKKELLKKINP
                     EKNHIQT"
     gene            4175..5407
                     /locus_tag="PK134_01345"
     CDS             4175..5407
                     /locus_tag="PK134_01345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB37530.1"
                     /translation="MENVDVLIIGAGPSGAVASAYLNKQNIKVLVLEKQKFPRFVIGE
                     SLLPHCMDHLSEVGFIDNIKKHGFQEKTGAAFYKGKHKTEFFFNDQFSDGWSWTWQVK
                     RADFDNILISTAIEQGVEVKFECEVNSVAASASEQTTTYTDKSGKTHTVKSKYIIDAS
                     GYGRVLPRLFKLEEPVSTPPRGAVFCHIHDTKRTKKAGANIFIHSFNENSAWIWAIPF
                     SDKTTSVGVVSNVELVDEFAANNGEKFLNHIRTFGDLAGRFENEQLVFEPRTIHNYAV
                     RSKQLFGPGYVLCGNSTEFLDPVFSSGVTLATGSGLLAAKLTEKVLNGEQVNWSEVYE
                     EKLRRGIDVFRTFVLGWYNGDLQTIIYSNDIDPNIKKQICSVLAGYVWDTENPVVKKH
                     KTLIPTLAEVIRIKEKEA"
     gene            complement(5411..6541)
                     /locus_tag="PK134_01350"
     CDS             complement(5411..6541)
                     /locus_tag="PK134_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRB37531.1"
                     /translation="MIIGAGPAGSACVMALKDAGLNIVWIDKATFPRDKVCGDAIPSN
                     VQRVLKTIDVSLNEKFLQQFIEKVQVEGCRFVAPDKSSFDLTFVTKGHAAKRLHFDHF
                     LFDMANECNSHVTFIGNASVELIVNSATAVTVKLTDGRTIEGQIIIGCDGANSIVRKN
                     LAQTFIRKKENIAAVRAYYNNVADLEHNMLEIHIVKGYMPGYFWVFPLPENQSNVGFG
                     MVNRFITNRKIDLKEALKEIIQNDKAISPRFSQATTDGVISGFGLPCGGRKNPISGQR
                     FLLCGDAASLINPATGEGIGNAMISGRIAALHIINCFQANNFTQSFNAQYDVLVYDKL
                     LGDLRIQHWLQRITADREWLINFALKHVSNNNWIRQKVRKFF"
     gene            complement(6565..7272)
                     /locus_tag="PK134_01355"
     CDS             complement(6565..7272)
                     /locus_tag="PK134_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRB37532.1"
                     /translation="MGRIFEKRKHKMFVRYAKMAKAFTRIGKEIAIAVRQGGPDPHYN
                     PKLRQVIQNAKGLNMPKDRVDAAIKRATSKDMKDYEEITYEGYAPFGVAIMVECATDN
                     STRTVANIRMHFNREEGSLGTSGSVGFMFERRGLFKFSAEGKNIEDLELELIDFGLIE
                     IWEEDGFIYLYCNFEDFGAMTKVLEDRSIEIINSELTRVPLTLTEVTEEQEHKIQVLI
                     DRLEDDEDVQSVFHNMK"
     gene            7384..7781
                     /gene="ssrA"
                     /locus_tag="PK134_01360"
     tmRNA           7384..7781
                     /gene="ssrA"
                     /locus_tag="PK134_01360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7901..8452)
                     /locus_tag="PK134_01365"
     CDS             complement(7901..8452)
                     /locus_tag="PK134_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRB37533.1"
                     /translation="MKLSSAQTKQLLSTLENRFTENMKRHKGLQWGNVETLLRKSSSK
                     LSVLWQMEETGGQPDVVAFDKKSGQFTFFDCSPESPAGRRSLCYDNKALNDRKENKPK
                     GSALGLATEMGVEILSEEQYKFLHTLGNFDLKTSSWLFTPLHIRVLGGAIFGDCRYQQ
                     VFTYHNGAESYYAARGFRSFICV"
     gene            complement(8588..10636)
                     /locus_tag="PK134_01370"
     CDS             complement(8588..10636)
                     /locus_tag="PK134_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37534.1"
                     /translation="MYIFPSQSLLADEFNAVKANIAQSCLTPAAAQEILNLTPSTDRN
                     YIELQLGRTEEMRRLLADAEPFPVSGYCDVTKELQLLKIENSVLLPSQALQLAALTHT
                     VGLIIKFFNQAPRYPLLTQILNDCFFEPAIASEVERVLDTFGVVRSSASTELLHIRRT
                     LQRQRAESDRAYQQVIARYKKNGWLTDSEESSRNGRRVLSIVAEQKRTINGVIHDVSA
                     TGKTAFVEPDEVLHINNHIQQLEQDERLEITRIMRQLTVFLRNYYTTLQQYAFILTQM
                     DMHHAKALFALNYRAVKPEITNNPEINLINASHPILLIQQKDIQKSVVPFTLHLNHDK
                     RILIISGPNAGGKTVCMKAVGLLLIMAQSGIHITAKTGTTVGVFNKLLIDIGDSQSIE
                     YELSTYSSRLKNMKVFFEQVDNKSLFLIDELGSGTDPQLGGALAEAMLETLNQNHSFG
                     IVTTHYMNLKVLAEKVPGFVNGSMSFDKRNLKPLFQLQIGKPGSSYTFIVAERSGLPQ
                     QIINSARAKVPKSHMLLEKLLSKVEKDKIVIEEKLKAILQKEKTLNTLIKQNDELIYK
                     NENLKNSLQQHLRSHQENLDRKAEARIKKFALDLKQAKNKKAVIEKFLSEAGLKNKNK
                     EKVVHRTIDPRIISGTTVKLYNGKVSGIVQSVDQENATVLFGNFTTKCKLTDLILDSK
                     "
     gene            complement(10760..12136)
                     /locus_tag="PK134_01375"
     CDS             complement(10760..12136)
                     /locus_tag="PK134_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRB37535.1"
                     /translation="MNHSQSNNLNIHFLFIAVALSVLIAGVLFGVVGGIQYIVPGWLK
                     DELSFERVRPLHVTMVISWIFAGAMGGIYYYLPQTVKGKLYSPTLAVAHLVLFILTGL
                     SIIACYFAGVFGGREYFEFPPAIAIPMLVTWLLFVANMLLTLKRYYNSNTLIFSKVYI
                     WMWLTGMFFFLLTFLESYLWLFSFFSNNLVRDITVQWKASGAMVGSWNMLVYGTAFYV
                     MEKMSGDEKVATSKMTFFFYFLGLTNLMFNWGHHTYIVPASPWIRDIAYVISMTELLI
                     LGNIIWGWRKTVSSATKTFHLMPYRFFTASDVWIFINLILAIAISVPFINRFTHGTHI
                     TVAHAMGTTIGINTFILFASVFYLLRLPEGTTSLKYIKAGFWTLNVSLLIFWVSLIAS
                     GVTKTIELSNGGTFASAMQKMLPYFYSFFIAGIGIFAGILLIAIPALISCIQNVRQNR
                     LVSIQSVA"
     gene            complement(12165..13187)
                     /locus_tag="PK134_01380"
     CDS             complement(12165..13187)
                     /locus_tag="PK134_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB37536.1"
                     /translation="MKLKVIFTILIVGLLSVLACKKDKDDDPINNLPPYNPTPYTINT
                     PSNLPAMNVPSFNPTTVEGVYLGRKLYYDKLLHPTGTSSCSSCHIQSQGFAVTGTNII
                     PHVNLGYQNKFLWNGEIQGTLEDAMMFEVETFFNTDISRLQKDSLYPILFYKAFGSTT
                     IDTKKCAYALAQFLRTVVSGNSKLDRFIRHETLFTADEIQGFNIFFSEKGDCFHCHSL
                     SLTSDGDLHNIGLDSVFTGVNAGYYNVSHNAADYGKFRTPSLRNTALKTTFMHDARFT
                     TLDQVIEHYNTGVKHSPSLDPVMTKPGKELGLQLTPLEKQKLKAFLLTLTDSTFLNNP
                     DFSNPN"
     gene            complement(13189..13914)
                     /locus_tag="PK134_01385"
     CDS             complement(13189..13914)
                     /locus_tag="PK134_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37537.1"
                     /translation="MRTSILLIRNVILIALMTIMVSSCKKKDDDENKLNLNLSFAVDG
                     SKLEFNSIKYVNAAVNNYSVSKLHFYISAFEFENTDGNKFSFDSIFYVDAATQAPSFT
                     LEGLPVGNYKSLRFLIGLDSLHNISHALPNTIDNENMSWPDMMGGGYHFMKLEGNFMD
                     GGNPTGYAMHLGKNVHVVQVNIQKDFKVSGGTAALNLEMNINEWFSNPHNYDFNIDGN
                     YSMSDSVAMSKLALNGKDVFNIQ"
     gene            complement(13954..14328)
                     /locus_tag="PK134_01390"
     CDS             complement(13954..14328)
                     /locus_tag="PK134_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB37538.1"
                     /translation="MQVSLKFKVLITLFTLFSFYTAFVYTKATAGATFTMNDDAFKGK
                     MLYQKFNCTACHQLYGLGGYLGPELTSAYSQKAQNDAYFSAILKNGTVRMPDFNLSDK
                     EVNQVIEYLKYIDQTSKHISAY"
     gene            complement(14513..15028)
                     /gene="paaJ"
                     /locus_tag="PK134_01395"
     CDS             complement(14513..15028)
                     /gene="paaJ"
                     /locus_tag="PK134_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRB37539.1"
                     /translation="MVNDSLKEQSSLESSEVFQLLYAIPDPEVPVITIGELGVLRNVE
                     MINGRIVIYITPTYSGCPAMFAIESEIITILKENNISDFKVKTVHFPAWTTDWLTDAA
                     KKKLSDYGIAPPEHSSQIKHLRPDEVSLKCPFCQSDETHLTSWFGSTACKALYYCDNC
                     RQPFEHFKCHH"
     gene            complement(15022..15777)
                     /gene="paaC"
                     /locus_tag="PK134_01400"
     CDS             complement(15022..15777)
                     /gene="paaC"
                     /locus_tag="PK134_01400"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRB37540.1"
                     /translation="MNKNLFEYTLRLGDTSLILSQRLSEWCGHGPMLEEDIALTNIAL
                     DLLGQAKAFLSYAGDIENKERGEDDLAFHRDANQFRNLLLTEQPNVDFGFTIIRQLFV
                     STWQQYLYSELTHSTDKNISALAIKSLKEVNYHLRFASDWTLRLGDGTEESHQRVQNA
                     VNELWNYTGDLFEIDQVDKELLKHGIAADMKKVEESWNKHIKQVLNDATLSIPEINNF
                     MRTGSRKGVHTEHIGFILAEMQFLPRAYPDAKW"
     gene            complement(15783..16070)
                     /gene="paaB"
                     /locus_tag="PK134_01405"
     CDS             complement(15783..16070)
                     /gene="paaB"
                     /locus_tag="PK134_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRB37541.1"
                     /translation="MSDIQLKLWEVFTQKKTGMSFEHAGSVRAADKEMALLNARDVYS
                     RRGEATCIWVVPTECISSSSPEDVASFFEPSDDKIYRHPNFYKTPDGIKQV"
     gene            complement(16111..17055)
                     /gene="paaA"
                     /locus_tag="PK134_01410"
     CDS             complement(16111..17055)
                     /gene="paaA"
                     /locus_tag="PK134_01410"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRB37542.1"
                     /translation="MEVNIEQLEKDFQAKIDAGINIEPKDWMPERYRHQLIRMMSQHA
                     HSEVVGMLPEGNWITRAPSLMRKMVLLAKVQDEAGHGLYIYSATETLGISRDEMTNQL
                     LTGKAKYSSIFNYPTLNWADMGAIGWLVDGAAIVNQTALAKCSFGPYARAMLRICKEE
                     NFHQKQGYQILANMMQGTEEQKQMAQDSVNRFWWPSLMMFGPSDKNSPNSDELMRWKV
                     KLYSNDDLRQRFIDRTVPQAEAIGIKIPDNKLKWNEKTRHYEWGEINWDEFYAVIGGN
                     GPCNRLRMKQRTDAHKNGQWVREAANAFAEKRKKSKAA"
BASE COUNT         5413 a         3142 c         3094 g         5476 t
ORIGIN      
        1 cagcataaga caataaaatg gtttaaactg ccggtcatta atccatcaat agttaaatat
       61 gcaattattg ccattttact aataactgta tatttttatt ttgaagcatc aaacattcgt
      121 ttcaacagcg atgttgaaac tttaagcttt catcctactg agttgaaaaa taaagaagct
      181 gaacttacag gcataaatcc gtctattgaa aaaaagattt acctgattgc ttcagctaaa
      241 aattataact ctgcagttga tgcaaattac aaagcaggaa cattaataaa gaatttaata
      301 acacatggca tcataaaaaa cactttatct acagctgatt attttattcc ggattctctt
      361 tgtaaacaac gtcagctaag atggaattac ttctggcaac agaataaaac ccgtgtcacc
      421 aatcagataa aactatctgc cgatgaagca ggcttcagca acaatgcttt tgctccattt
      481 caagaaatga ttgacgatag catctatcaa ccattgtcaa aagacttact caaaacaatg
      541 ggcttagacc taatgtttga ttcaagtgca aaagaatctg tttttatttc atcttttaca
      601 gtggcaacat cacagaaaga ctctgtgaaa gctttattta aagatatccc gcatcttacc
      661 gtatttgaca gaagtgaaat ggcatcactg ctgcttgagt cggtgcgcag cgatttcaac
      721 taccttctgt acctcacagc aggcatggtg ttttttactt tattgcttat ttacggcagg
      781 gttgagcttg cccttcttgc atttttgcca atggttgtta gttggatttg gatattagga
      841 atgtcgtcca cctttaatat tgaatttaac tttgtaaata ttgtaattac aactttcatt
      901 tttggtctcg gtgatgactt tagtatattc gttactgatg gacttttgaa ccgttataaa
      961 ttcaggaaag acaatctaaa atcctatcag gctgcaatca tcctgtctgc attgacagtt
     1021 attgtgggca ccggtgtatt atattttgct aagcaccctg caattcattc cattgctgca
     1081 ttaagtgtta ttggcatcac ctgcatctta ctgatttcat ttcttgttca accatttatt
     1141 tttaaacttt ttgtagaacg cagaattaaa aatggacaca atccaatttc atttctacaa
     1201 ctttttatgt ccgttttttg tttcacctat tttattgttg gttgccttac tttctatcct
     1261 gttttgcttc tcatcagcct attccctgca cctgtaaaaa ttaaaagaaa agcagtaaac
     1321 tttattgtat caaaattttc agcatccgta ctgtattctg gcattcatgt aaaaaagaaa
     1381 ttttataacc ttgatcaact tgatttcaaa aagccttcaa ttattattgc caaccactct
     1441 tcttttctcg atattatgat tatgctaatg ttaaacagaa aagttattat catggcaaaa
     1501 gattgggttt accgttctcc tctattcggt ttggtggttc gctatgtcgg ttatattttt
     1561 tctgaagatg gaactgaaga taatataacc aacattaaag ctaagatgga tgaaggttat
     1621 tccattatga tttttccgga aggctcgcgt tcatctgatg gaaatcttaa tcgctttcat
     1681 aaaggagcct tttatcttgc agaaaaactg aatgctgata ttcaacccgt gatgattcat
     1741 ggtgcaggcg atgtttcacc caaaaatgaa atgatggtta aagatggcac cattaatgta
     1801 aaagctttac caagaatatt tgcaaatgat ttgagttggg gtaacgatta cagaagcagg
     1861 caaaaatcca tttcacaata ttttaaagag cagtacagac tttttaaaga tgaacgcgaa
     1921 gatgcagact atttatggca aaaagtttat tacaactata tttttaaagg cccggtttta
     1981 gaatggtatg gtcggataaa atggaatctc gaaaaaaaga attacgattt ttataataac
     2041 cttattggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atttatggac
     2101 ttgtttctgc attacaaaaa tcgtgaaaga aagatatcgg cttttgatta tgatgatgat
     2161 aaaattgcca ttgctgccaa tgtctggaat aagaatgcaa atttacaatt ctttgctgcc
     2221 gacataacaa aaacagaagt accctcatct gaggtaattt ttcttaatga cgtattacat
     2281 tacctaaata ttgacagtca ggaaaaagtg ctgcagaaat gtgcagatgc cctgatgcct
     2341 ggcggaataa tgtttattcg tgatggtgtt gctgatgata ttaaacatag aaacactaaa
     2401 tttactgagt tattatctac aaaattcttt tccttcaata agaaaatgaa tgacttaaat
     2461 tttttcaaaa ctgagtatat ttatcaattt gcaaaaagaa acaacctgaa aattgaagaa
     2521 cataaacatt ccaataatac ttccaaccga ctatttgtac tgagcaaatg aaaatcaatg
     2581 acaaaatagt ttatgatata gccataattg gtggtggttt ttcaggaatg tgttgtgctt
     2641 atatcttagc taaagaaggt tataaagtag ccttggtgga aaaaaatatt caattaggag
     2701 gaagccttca gattttcagc agagataaaa aaattttaga taccggcctg cattatattg
     2761 gcggctacga tgaaggagaa aatctctatc agttattcag ctattttggt ttgaaagaca
     2821 cactgcagac aaaaaggatg gatgataacg gttatgacta tgtcagtttc agtgatggca
     2881 gaaaatatgc tcatgccatg ggatatgata atttcattaa ttctttgtgt aaagattttc
     2941 ctgatgagga gaaggccatt catcggtttt gtgataaatt gaaagaatac tgccaaaaat
     3001 ttccactcta tgatctgagt aataaaataa ctgacgatta ttttaacgag gaacttagaa
     3061 atgaaaatgc atgggattat attgcttcca ttacagaaaa taaaacgctc agaaatgtac
     3121 ttgcaggcaa taacatgcta tatgcaggca taaaggagaa gacaccaatg tttgttcatg
     3181 cacttatttt taacagttac atttgtggaa gctacaagat tgtcaccggt ggtgcacaat
     3241 taattaaaca gtttgcaagt aatattcgca aagccggtgc tgatatttat actcataaac
     3301 agattacagg agctacatat aaggagaatc gggaaatttc tcagttgcta ttagaaaatg
     3361 gggagtgtat tacagcatta aaatatatct ctgccataca ccccaacgaa actatttcgc
     3421 tactcggcaa tgaccgcttt tctcatgcct gggttaacag ggtaaaacaa atggatcaaa
     3481 ctgtttctgc attcttagta caaattactt ttaaaccaaa atcatttcca tacatgaact
     3541 acaacatgta tcattttttt aatgatgatg tttgggacac tattaattac acagaagaca
     3601 attggccatt aagtttcttt gtgtttacac cggttccatt aaaagacccg cagtatgctg
     3661 aatccatttc tgtgatgtgc tatatgaaat ctgatgatgt tgcgcaatgg aaagatagtt
     3721 ttaataccac aacacatcca tcaaaccgca gcgaaagcta tcaggaattt aaaagaagaa
     3781 aagaagaaaa agttcttgaa aaattagaaa cactttttcc ggaaataaga aaggctatac
     3841 tgaatgttta cagttcaaca cctcttactt ttaaagatta tactgccagc actgatggtt
     3901 cgatttacgg agttttaaaa gacagcaatg ctcctttgaa aagtatgtta caaccaagaa
     3961 caaaaattcc taatctttgg ctaacaggac aaaatatagt ttttcatggc attttaggta
     4021 ctgcaataag tgctttgcta acatgctttg aatttactga caaaaaagaa ctgttaaaaa
     4081 agataaatcc tgaaaaaaat catattcaaa cataaaagtc aaattaggtt tactattaaa
     4141 tttgcttcgc aataaaaatt tcaacaacca ttaaatggag aatgtagatg tactgataat
     4201 tggtgcagga ccttccggtg ctgttgcctc tgcctatctg aataaacaaa acataaaagt
     4261 gcttgtgctg gaaaagcaaa aatttccacg ttttgtaata ggggaaagtt tactgcctca
     4321 ttgtatggat catctatctg aagtaggctt tattgacaat atcaagaaac atggatttca
     4381 ggaaaaaaca ggtgctgcat tttataaagg aaaacataag acagagtttt tctttaacga
     4441 tcagtttagc gatggatggt cgtggacatg gcaggtaaaa cgcgctgact ttgataacat
     4501 actaatttca actgctatag aacaaggtgt ggaagtaaaa tttgaatgtg aagtaaattc
     4561 tgttgctgct tctgcaagtg aacaaacaac aacctataca gataaatccg gaaagacaca
     4621 tactgtaaag tcgaaataca ttattgatgc cagtggatat ggtcgtgtgc taccacgtct
     4681 atttaaattg gaagagcctg tgagtactcc accccgtggt gctgtttttt gtcacattca
     4741 tgacaccaaa cgaacaaaaa aagcaggtgc aaatatattt attcattcat ttaatgaaaa
     4801 cagtgcctgg atttgggcta ttcctttcag cgataaaact acctctgtag gcgtagtaag
     4861 caatgttgaa ctggttgatg agtttgctgc aaataatggt gagaagtttt tgaatcatat
     4921 cagaacattt ggtgacctgg caggacgatt tgaaaacgaa cagttggttt ttgagccacg
     4981 cacaattcat aattatgctg tgcgttcaaa acagttgttt ggtccaggat atgttctttg
     5041 tggtaacagc acagaatttt tagatcctgt attttcatca ggcgtaacgc ttgcaacagg
     5101 ctccggttta ctagcagcta aactgacaga aaaggtattg aatggcgagc aagtcaattg
     5161 gtctgaagtt tacgaagaaa aattgcgcag aggcatagac gttttcagaa catttgtatt
     5221 gggttggtat aatggcgact tacagaccat aatttactca aacgatattg acccgaatat
     5281 aaaaaagcaa atttgttctg ttctggcagg atatgtttgg gatacagaaa atcctgttgt
     5341 taagaaacac aaaaccttga taccaacgct tgcagaggtt atcagaataa aagaaaagga
     5401 agcgtaattt tcagaagaat tttctaactt tttgccttat ccagttattg ttgctgacat
     5461 gcttcaatgc aaaatttatt aaccactctc tgtctgcagt aatgcgttgc agccaatgtt
     5521 gaatacgtaa atcgccaagg agtttatcat ataccaacac atcatattgt gcattgaacg
     5581 attgcgtaaa gttgttcgcc tgaaaacaat tgatgatatg taaagcagct attcgtccac
     5641 ttatcattgc attgcctata ccttcacctg ttgcaggatt gatgagtgaa gctgcatcac
     5701 cacagagtaa aaatctttgt ccggaaattg ggttttttcg tccaccacat ggaagcccga
     5761 atccagaaat cacaccatca gtagttgcct gactaaagcg agggctaatc gccttatcgt
     5821 tttgaataat ttcttttaaa gcctctttta aatctatttt acggtttgtg ataaaccggt
     5881 taaccatgcc aaagccaaca ttggattgat tttccggcag aggaaaaacc cagaaatatc
     5941 caggcatata acctttgaca atatgaatct cgagcatatt atgctcaagg tctgcaacat
     6001 tattatagta tgcacgaaca gctgcaatat tttctttttt acgaatgaat gtttgagcaa
     6061 gattctttct gactatggaa tttgcaccat cgcagccaat aattatctga ccttcaattg
     6121 tccttccatc agttagttta acggtgacgg cagttgcgct gtttacgatt aattcaacag
     6181 atgcattgcc aataaaagta acatgactgt tacattcatt tgccatgtca aaaagaaaat
     6241 gatcgaaatg taagcgcttg gcagcatgac ctttggtaac aaatgtaagg tcgaacgaag
     6301 atttatcagg tgcaacaaaa cggcaacctt ctacctgaac tttttctata aattgttgta
     6361 aaaatttttc gttgagagaa acatcaatgg tctttaacac gcgttgcaca ttggaaggaa
     6421 tggcatcacc acaaactttg tcacgtggaa aagtggcttt atcaatccag acaatattga
     6481 ggccggcatc tttaagtgcc attacacagg cagaacctgc aggccctgct ccaatgataa
     6541 ttacatctgt tttgatgtct taatttattt catattgtga aaaacacttt gcacatcttc
     6601 gtcatcttcg agcctgtcaa taagcacttg aattttatgt tcctgctctt cagttacttc
     6661 ggtaagtgtt aatggaacac gtgtaagttc gctgttgatg atttcaatag agcggtcttc
     6721 aagaaccttt gtcattgcac caaaatcttc aaagttgcaa tagagataaa tgaatccgtc
     6781 ttcttcccaa atctcaatca aaccgaagtc tataagttca agttcgaggt cttcaatatt
     6841 ttttccttca gcagagaact taaacaaacc acgtctttca aacataaatc ctacagagcc
     6901 tgaagtgcct aaacttccct cttcgcgatt gaagtgcatg cgtatgttag caactgttct
     6961 ggttgaatta tcggtagcac attctaccat aattgcaacg ccaaagggag cataaccttc
     7021 atacgtaatt tcttcatagt ctttcatgtc cttgctggta gcacgtttta tagcagcatc
     7081 taccctatcc ttgggcatgt ttagaccttt tgcattctgt attacctgac gcaatttagg
     7141 gttgtagtgt gggtcggggc caccctgacg aacagcaata gcaatttctt ttcctatgcg
     7201 cgtaaatgcc ttagccattt tagcatagcg tacaaacatt ttatgttttc tcttttcaaa
     7261 aattcgtccc ataatgtaaa acggtttgct gcaaaaatac acttctgaaa aaactgacag
     7321 caaaatattc ataaatgtgt catagaatag ctgtaaattt gcattctgtt ctttgataat
     7381 tatggggccg aaaggtttcg acagtgtgcg taataaatac gtaagcatgc cgggtgctgt
     7441 gagaacgacc cgtaaatccc gattcacaac tcgcttaaat ggcgaaacaa actacgcgct
     7501 tgcggcttaa ttcttccaag aagaattgcc tgatgctgcc accaggtggt agcacaagct
     7561 gtcctacgca gacttaagtt catagcgttt gcgaaaaggg catcacagat atgaactggc
     7621 tgctgttgtg ctctgcaaca tcagtgagaa aacaagggct aaggatagaa ctgacaggca
     7681 ggtagtcttg ttttatccct acaattaaat atctgctaag catgtagaaa gtgtatggat
     7741 tccatatctg gacgtgagtt caaatctcac cggctccact ttttttgaat tgcattgagg
     7801 agttagaaaa tgatgtgtta tgaggtgatg aagtaatttg ataatgtaga atgcagaatt
     7861 ctgaattgca gttgcgttaa gcgcttataa tgtaatgggc tcaaacacat ataaaacttc
     7921 tgaatcctct tgctgcataa taagattctg caccgttatg ataagtaaaa acttgttggt
     7981 agcggcaatc tccaaagatg gctcctccta aaacacgaat atgtagtggt gtaaataacc
     8041 acgaagatgt tttaagatca aagttaccca gtgtatgcag aaatttatat tgttcttctg
     8101 ataaaatttc aacacccatt tcagttgcta acccaagtgc acttcctttg ggtttatttt
     8161 ctttacggtc gtttaatgct ttgttgtcgt agcaaagact tcttctgccg gctggacttt
     8221 cgggagaaca gtcaaagaat gtaaactgac ccgatttttt atcaaaggca acaacatcgg
     8281 gttgtccacc tgtttcttcc atttgccaaa gtacgcttaa tttagaagac gattttctga
     8341 gcaatgtttc tacattaccc cattgcaggc ctttatgacg cttcatattt tctgtgaatc
     8401 tgttttctaa agtagataag agttgttttg tctgtgctga agataatttc ataagtacta
     8461 aaaatttgat tttatgtttg atgaaaactg aataagggtt gtgaaattaa aaattatctt
     8521 ttgatttttt atagtgtaca attgcaatca tttaataacg gttgaaaatt ttactacagt
     8581 atgatattta tttactatca agtatcaggt cggttaactt gcactttgta gtgaagtttc
     8641 caaatagaac ggttgcattt tcctgatcaa cagattgaac aattcctgat acttttccat
     8701 tataaagttt cacagtagtg ccactaataa tacgtggatc aatggttctg tgaacgacct
     8761 tctccttatt cttgttttta agccctgctt ctgaaagaaa tttttcaatt acagcctttt
     8821 tattctttgc ctgtttaaga tctaatgcaa acttcttaat acgtgcttca gcctttctat
     8881 caagattttc ctgatgactg cgcaaatgct gctgaaggct attcttgaga ttttcgtttt
     8941 tataaattag ttcatcattc tgttttatga gcgtgttaag tgtcttttct ttttggagaa
     9001 ttgcttttaa cttttcttca atcactattt tgtctttttc aacttttgat agtagttttt
     9061 caagtagcat gtgtgacttc ggcacctttg cacgagcact gttgatgatt tgttgcggta
     9121 gcccgcttcg ttctgccaca ataaatgtat aagagctgcc aggcttacca atttgcagtt
     9181 gaaagagtgg ttttagatta cgtttgtcaa acgacataga gccattaacg aatccaggca
     9241 ctttctctgc aagtactttc aggttcatat aatgcgtagt tacaattcca aatgagtggt
     9301 tttggtttaa tgtttcaagc attgcttcag ccaatgcacc tccaagttga gggtctgtac
     9361 cactaccaag ttcatcaatc agaaagagtg atttattgtc aacctgctca aaaaacactt
     9421 tcatgttttt taatcgggaa ctgtatgtgc ttaattcgta ttcgatagat tgagaatcgc
     9481 ctatatcaat aagaagttta ttgaaaacac caactgtagt tcctgttttt gcagtgatat
     9541 gtattcctga ctgcgccatg ataagcaaaa gcccaacggc tttcatgcat actgttttac
     9601 ctccggcatt aggtccgctg atgattaata ttctcttgtc gtgattcaga tgaagcgtaa
     9661 atggaacaac acttttttga atatcctttt gctgaattaa caatatcgga tgcgaagcat
     9721 tgataagatt tatctcaggg ttattcgtga tttcaggttt tacggcacga tagttcagtg
     9781 caaaaagtgc tttggcatga tgcatatcca tttgagtgag gataaatgca tattgctgaa
     9841 gtgtggtata ataattacgc aggaatacag taagttgtcg catgattcga gtgatttcaa
     9901 gtctctcatc ctgctctaac tgttgaatat gattatttat gtgcaacact tcatcaggct
     9961 caacgaatgc agtttttcct gttgcagaaa catcatggat gacaccattg attgtccgct
    10021 tttgttctgc aacgattgaa agaacccgtc ttccgttgcg tgatgactct tcgctatctg
    10081 taagccatcc atttttctta tagcgtgcaa tgacttgctg ataggctctg tctgattcag
    10141 cacgttgcct ttgtaaagta cgtctgatgt gcaagagttc cgttgatgca gaagaacgca
    10201 ccacaccaaa agtgtcaagc actctctcaa cttcacttgc aatggcaggt tcaaaaaagc
    10261 aatcattgag tatctgtgtg agtaaaggat atcgtggtgc ctgattgaaa aatttaataa
    10321 ttagacctac cgtatgtgtg agtgcagcaa gttgcaatgc ttgtgaaggc agcaatacgc
    10381 tgttttcaat cttaagcagt tgaagttctt tcgtaacatc acaatagccc gaaacaggaa
    10441 aaggttctgc atcagcaagc agcctacgca tttcttctgt tctcccaagt tgtaattcaa
    10501 tataatttct atcagtactt ggagtcagat taagaatttc ttgtgcagca gcaggtgtaa
    10561 gacaactctg ggcaatgttt gcttttacgg cattgaactc atccgcaagt aacgactgag
    10621 atgggaaaat atacatgtaa acaattcaaa ataaattttc agttttatgc ataaacttaa
    10681 ggtatgccca atactgaagt gcgaaattaa tacatggcca tgataccaaa caaattatta
    10741 aatttatatg agataattat caggcgactg actgtataga aacaagtctg ttctgtctga
    10801 cattttgaat gcatgaaatc aaagccggaa tagcaatgag taaaatgcct gcaaaaatgc
    10861 caattcctgc aatgaaaaag ctataaaaat acggtagcat tttttgcata gctgatgcaa
    10921 atgttccccc attactcagt tcaattgttt tagtgacgcc tgatgcaata agtgaaaccc
    10981 aaaatatgag caaagaaaca ttcaatgtcc agaagcctgc ttttatatat ttaagagagg
    11041 ttgtgccttc tggcagtctt aatagataaa acaccgaagc aaaaaggata aatgtgttga
    11101 ttccaatagt agtacccatt gcatgtgcaa ctgtgatgtg ggtgccatga gtgaaacgat
    11161 ttataaacgg aacagaaata gctatggcta aaataaggtt gataaatatc caaacatctg
    11221 aagccgtaaa aaaacgataa ggcataagat gaaaagtttt agtagctgat gaaaccgttt
    11281 tgcgccagcc ccagataata tttccaagaa taagtaactc tgtcatactt atgacatagg
    11341 caatatcccg aatccagggc gatgcaggca caatgtaggt atgatgcccc cagttaaaca
    11401 taagattggt aagacctaaa aaataaaaaa agaaggtcat ctttgatgtg gcaacctttt
    11461 catcaccact cattttttcc attacataaa atgcggtacc atataccagc atattccatg
    11521 agcccaccat agctccggat gctttccatt gaacggttat atcacgaaca agattattac
    11581 tgaaaaaaga aaacaaccac agatatgatt ctaagaatgt tagcagaaag aaaaacattc
    11641 ctgtaagcca catccagata taaactttag agaagatgag tgtattgctg ttgtaatatc
    11701 gtttaagcgt cagcaacata ttggctacaa acaacagcca ggttacaagc attggaattg
    11761 caattgccgg tggaaactca aaatactctc gaccaccaaa tacgcctgca aagtagcagg
    11821 caatgatgga taatcccgtc agaataaata atacaagatg ggctactgct aaagttggtg
    11881 aatatagttt acctttcacc gtctgaggta aataataata gataccgccc atggctccgg
    11941 caaaaatcca tgatattacc attgtcacgt gaagtggtct gactctttca aacgacaatt
    12001 catctttgag ccaaccgggt acgatatact gaatgcctcc aactacgcca aacaacaccc
    12061 ctgcaataag tacagaaagt gcaactgcaa taaacagaaa atggatattt aaattgtttg
    12121 actgactatg gttcattgta tgtattactg aatcacgcta aaactcagtt tggattagaa
    12181 aaatctggat tgttaagaaa agttgaatca gtaagtgtaa gtagaaacgc ttttagcttt
    12241 tgtttctcaa gtggtgtaag ttgtaaacca agttctttgc cgggctttgt cataaccgga
    12301 tctaaagatg gtgaatgttt aacacctgtg ttgtaatgtt caattacttg gtcaagtgta
    12361 gtgaaacgtg catcgtgcat gaaggttgtt tttaatgctg tatttcttag cgatggtgta
    12421 cggaactttc cataatctgc agcattatga ctgacattat aataccctgc attgacacct
    12481 gtaaaaactg aatcgagtcc gatattgtgc aaatcgccat cggaagttaa agacaacgaa
    12541 tgacagtgaa aacagtcgcc tttttctgaa aagaaaatat taaatccttg tatttcgtca
    12601 gctgtaaaca aagtctcgtg acggatgaaa cgatctaact tagaattgcc ggaaacaaca
    12661 gttctcagaa attgggccaa tgcataagca cactttttag tatcaatagt tgtactgccg
    12721 aatgctttat aaaaaagaat aggatataag ctatctttct gaagtcttga aatatctgta
    12781 ttaaaaaaag tttctacttc aaacatcata gcatcttcta atgttccctg aatttcgcca
    12841 ttccataaaa acttattttg atagcctaag ttaacatgtg gaataatatt cgtgcctgta
    12901 acagcaaagc cttgcgactg aatatggcat gatgaacatg atgaagtacc tgtaggatgt
    12961 aaaagtttat catagtataa ctttcgccct agataaacac cttcaactgt agtagggttg
    13021 aatgatggaa cattcattgc cggtaaattt gaaggtgtgt tgattgtata tggtgttgga
    13081 ttgtaaggag gaaggttatt tataggatca tcatccttat cctttttaca ggcaagtaca
    13141 gagagcaacc caacaatcaa tatagtaaaa attactttta gcttcatact attggatatt
    13201 aaaaacgtct tttccgttta atgctagttt tgacatggca acgctgtcac tcattgaata
    13261 atttccatca atgttaaagt cgtagttatg cggattagaa aaccattcat taatattcat
    13321 ttccagatta agtgctgctg taccgcctga aactttaaaa tctttctgaa tgtttacttg
    13381 caccacatgc acatttttcc ctagatgcat tgcatatccg gttggatttc caccgtccat
    13441 aaaattacct tcgagtttca taaaatgata cccacctccc atcatatcag gccaactcat
    13501 attttcattg tcaattgtat ttggcaaagc gtggctgatg ttatgcaaac tgtcgagacc
    13561 aattaaaaaa cgaagtgatt tataatttcc aacaggcaat ccttcaagcg taaaagatgg
    13621 tgcttgagtt gcagcatcaa cataaaagat actatcaaaa gaaaacttat ttccgtcagt
    13681 attctcaaac tcaaatgcag aaatatagaa atgcaatttc gaaacactgt aattgttgac
    13741 tgcagcattt acatacttta tgctattaaa ttcaagttta ctaccatcca ctgcaaaaga
    13801 taaatttaga ttgagtttgt tttcatcgtc atcttttttc ttacaagatg aaaccattat
    13861 ggtcatcaag gcaatcaata tgacatttcg tattaataat attgaagttc tcatacttta
    13921 ttgattttat tgcttgtctt catgttcagg tttttagtat gcagaaatgt gttttgatgt
    13981 ttgatcaatg tatttcaaat actcgattac ctgattaacc tctttatcac tcaaattaaa
    14041 atcgggcata cgtacagttc catttttgag tattgcagaa aagtatgcat cattttgtgc
    14101 tttctgcgaa taggctgatg taagctctgg gccaagatat ccacctaagc cataaagttg
    14161 atgacatgca gtacagttga atttctgata gagcattttc cctttaaacg catcatcgtt
    14221 cattgtaaaa gtggcgcctg cagttgcctt agtgtaaaca aatgcggtgt aaaaactaaa
    14281 taatgtaaaa agtgtaataa gtaccttaaa ctttaaactc acttgcatag ctgaatcgtt
    14341 tttgcatttc cataatcaaa gaaatgctga atcatcatat acaaatgaac aattatcagc
    14401 atgaaaacaa attgactaat gacagtgaca aggctgactt ttatcactgt ttaaaaaagt
    14461 atcagaatag actgaaaaat aaatatttaa acgagcctgt taatagaaaa tattaatgat
    14521 ggcatttaaa atgttcaaat ggttgcctgc aattatcaca ataataaaga gccttgcatg
    14581 ctgttgagcc aaaccaactg gtaagatgtg tctcatctga ttgacaaaaa ggacatttta
    14641 aacttacctc atcaggacgc aaatgtttta tttgtgacga atgttcaggt ggcgcaatgc
    14701 cataatcaga aagcttcttt ttagcagcat cagtaagcca gtcagtagtc catgcaggaa
    14761 aatgtactgt ctttacttta aaatctgaga tgttattttc tttaagaata gttataattt
    14821 ctgattcaat tgcaaacata gcagggcaac ccgagtatgt tggtgttata taaatcacta
    14881 tcctgccgtt tatcatttca acattgcgaa gcacacccaa ttctccaata gtaattaccg
    14941 gcacctcagg atcaggaatg gcatacagta actgaaaaac ttctgatgat tctaaactgc
    15001 tttgttcttt caaggaatca tttaccattt ggcatcagga tatgcacgag gtaaaaattg
    15061 catttctgca agaataaatc ctatatgttc tgtatgtact cctttgcggc ttcctgttcg
    15121 cataaaatta ttaatctcgg gaattgataa cgtggcatca ttcagcactt gtttgatatg
    15181 tttattccaa ctttcttcaa cttttttcat atcggctgca ataccgtgtt ttaacaactc
    15241 tttatcaact tggtctatct caaaaagatc accggtataa ttccaaagtt cattaaccgc
    15301 attctgcact ctttgatgac tctcttcggt gccatctccc aaacgcaatg tccaatccga
    15361 agcaaaacgc aaatggtaat taacttcttt caatgattta attgcaagtg cactgatgtt
    15421 tttatcagta gaatgtgtca attcagaata taaatattgt tgccatgtac ttacaaaaag
    15481 ttgtctgatt attgtaaatc caaaatcaac attaggttgt tctgtaagca ataaatttct
    15541 gaattggttt gcatctctat gaaaggccaa atcatcttca ccgcgttctt tattttcgat
    15601 atcacctgca taggaaagaa aagcttttgc ctgccctaat aaatcaagtg caatattggt
    15661 aagagcaata tcctcttcaa gcattgggcc atgtccgcac cattcactca atctctgact
    15721 taaaatgaga cttgtgtcgc ctaaacgcaa tgtatattca aataaatttt tattcatgat
    15781 tattaaacct gcttaatgcc atcaggcgtt ttataaaaat ttgggtggcg ataaatctta
    15841 tcatcagaag gctcaaaaaa agatgcaaca tcttcaggtg acgaagatga aatacactca
    15901 gtaggaacta cccaaatgca ggttgcttcg ccacgtctgc tgtaaacatc gcgggcatta
    15961 agtaatgcca tttctttatc tgctgctctt acacttcctg catgctcaaa agacattccg
    16021 gttttctttt gggtgaacac ctcccataat ttcaattgta tgtcggacat gatttattaa
    16081 attaaaaaga taaatattga aattgattgt ttatgctgct ttactctttt tgcgtttttc
    16141 tgcaaatgca ttagcagctt cacgtaccca ctgtccattt ttatgagcat cggtacgttg
    16201 tttcattcta agtcggttgc aaggaccatt accaccaata actgcataaa actcatccca
    16261 gtttatttcg ccccattcat aatgacgcgt tttttcattc cacttcaatt tattatctgg
    16321 tatttttatt ccaattgctt ctgcctgtgg cactgttctg tcaataaaac gctgacgtaa
    16381 atcgtcatta ctatagagtt taactttcca acgcatgagt tcatcagagt taggagagtt
    16441 tttatcactt ggcccaaaca tcatcaatga tggccaccaa aaacggttaa ctgaatcctg
    16501 tgccatttgc ttttgttctt cagtgccctg catcatattg gctaaaatct gatagccttg
    16561 tttttgatga aaattttctt ctttacaaat acgcagcatt gctcttgcat agggaccaaa
    16621 ggagcatttt gccaatgcag tttggtttac aattgctgca ccgtccacaa gccatccaat
    16681 agctcccata tctgcccagt ttaatgtggg gtaattaaaa atacttgaat attttgcctt
    16741 acctgtcagt agttgattag tcatttcatc acgactaatg cctaaagttt ctgtggcact
    16801 gtaaatgtac agcccatgcc ccgcttcgtc ttgtactttg gctaataaaa ccatctttcg
    16861 cattaacgaa ggcgctcgtg ttatccaatt gccttcaggc aacattccaa caacctcgct
    16921 gtgggcatgt tgcgacatca ttcttataag ttgatgtctg taccgttcag gcatccaatc
    16981 tttaggttca atgttgatac ctgcatcaat ctttgcctgg aaatcttttt ccaattgttc
    17041 tatattcacc tccataaaat ttaatcaaaa caatagtagt aaccaaaagt tactttcctt
    17101 taaaaacagg gtttcttttt tctttaaaag ctggtgtggg gccaagtata gtaattttag
    17161 atgtat
//
LOCUS       DAORQT010000017        16761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_303_length_16761_cov_8.511852, whole genome shotgun sequence.
ACCESSION   DAORQT010000017 DAORQT010000000
VERSION     DAORQT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_303_length_16761_cov_8.511852"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /locus_tag="PK134_01415"
     CDS             <1..1892
                     /locus_tag="PK134_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB37543.1"
                     /translation="ISAPSGGVSLLNILWSDGSTSTSAQATAGTVMTLTVTDNAGCNR
                     DTTFSMLSTNGPQISSIQTTDIICFGDSSGSATVNITGGTPPISIQWIGTLPVQNTAT
                     AINLKAGTYTCIVTDVNGCSAQSVANINHLNTLITPVLAITNASCGNSNGQIVVTATG
                     GSGTYQYKLNNGVYSATNTFTNLAAGSDSVFVKDNNGCEVFVISTISNIQGPVISVID
                     SANVSCFGSNDGFIILNVTGAAQPITYSWSDGPTTLTRNNLGPGNYTFTAQDANNCIS
                     VRNFIITEPTAITISEIIPNKNGIYNITCNTLADGEIQVIANGGVPFTSTTAYNYQWS
                     ISGASGANVINLTAGPYTVTVTDSMGCSVTKSFLLTEPPALNAGPDFTQSICGTDTIV
                     LTGNIPTYGFGKWTVRSGGGLIAEPDSITTQITAIANGVNVFAWVVYDNFCSDTALYV
                     INKKEKIIADGGIDQNVCDNFVFLAAVPPPSGTGVWQSINGNSTIQNPSSPATKVENL
                     SPGKNIFVWTITNGSCTASDTVKIAILPPEECYTDVVMPTGFTPNEDGYNDIYVIRGL
                     DNTDNTLTIFNRWGNLVYEKKNYSNDWDGKSNNKQILPDGTYYAILRIPARNLVLKTY
                     IDLRR"
     gene            1897..2817
                     /locus_tag="PK134_01420"
     CDS             1897..2817
                     /locus_tag="PK134_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRB37544.1"
                     /translation="MKKLYSLIITLFMSGWLFGQQAPMTTQYMFNTLLINPAYAGSKP
                     FTSATMMVRKQWAGFKGSPTTETASIHGALEDKNVGLGLYISNDHAGITNRTELFGSY
                     SYTIEYGESKLAFGLQAGLSYLKSTLSDLVYNDNNDPVYDKNTVNNVLPNFGAGLYYH
                     TDLFYAGISAPLLLSYDPLKTLSVEMTDVHHIRRHYFLTTGYVFSLSEQVKLKPSVLL
                     KYVNGAPLQYDINANLLLNNIIWIGTSYRSNDALAIIGEYQLSKKLRIGYSFDYSLTI
                     LRNHSAGSHEFMLGYDFGYNVPKIKTPRYF"
     gene            2820..4700
                     /locus_tag="PK134_01425"
     CDS             2820..4700
                     /locus_tag="PK134_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB37545.1"
                     /translation="MIRKRKPYLLFVVLIFTSCLSVKQRTADTYYEQNLYDKAIPYYQ
                     EVANKKFNLDILIKLADCYRNVKNYPLSEQIYSKIVTSNSDPIYQFYYAEALMQNGKY
                     VEARKWLNKFLVLNRADSRALNLLASCDSIEMLYKDSLLFDVTRLPFNGIDESNFSPE
                     FFHSGIVYVSNRNIDAKSYKTNPVIDNYNLFYAQQTELGHWMDPVPLRGTINSIFNEG
                     PCTFNTEFTKAYFTRNANEGKKIITNQQSENVLEIFEATTKDGEWNISGPVSFNNNNY
                     SVGHPSISPSGNTMVFVSDMPWGYGGTDIYKVQWVNNTWSKPVSIGKTINSSGNEMFP
                     YLLTDSILYFSSNGMIGVGGLDIYRTELTADGWTVPENLGYPINSPRDDFGLICDNEE
                     LSGYFSSNRQQGKDNIFSFKRNPPVLTLKGKLIDKSTNKSLKNINITLTASSIDTVLT
                     TDSEGNFSAQLKNNKEYKLRAEDKSYFTASEKISTFGYRKSATLNKDLTLEKVIFNKP
                     VIWRGIAFEKGKSDLTPSTKTELNKLFNILLENKNIKIELSCHTDSRGIERDNLILSQ
                     ARADKAAAYLINKGISKDRIISVGFGSQRLLNNCVRGVLCLEEDHQVNIRTEIRYSPY
                     QR"
     gene            4734..6536
                     /locus_tag="PK134_01430"
     CDS             4734..6536
                     /locus_tag="PK134_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HRB37546.1"
                     /translation="MAIILGISAFYHDSAATIAIDGKIISAVQEERFSRIKNDASFPV
                     NAIKFCLLHSGLKLNQIDHIVYYEKPFLKLERLLETFLETAPKGLQQFTQAAPIWIKE
                     KVLIKNKIRESLHLIDENYSAKEILFCEHHLSHAASAFFTSPFPDAAIITTDGVGEWC
                     TTSIMNGKLNSITSIKELNFPHSIGLLYSAFTQFLGFKVNSGEYKVMGLAPYGNRNSA
                     QYKLYKNKISELVDLKTDGSVFLNMKYFNYLTGLNMINENLWSELFEIKPRTAESEIN
                     LNHCNLALAIQTITENVILNLAKEAKQITNSDNLCLAGGVALNCVAVGKLNKQNIFKK
                     IYVQPASGDAGGAIGAALAVEHIFLNNKRKVPEKDLMQGCRLGPSYDNHTVTQTLKKH
                     NLHFEEFKEGELLERTARHLANDKTIAWFQGRMEFGPRALGSRSIFANPATPQIQQTL
                     NQKIKKRESFRPFAPAMLLEDYLEFYEGGNENSFMSMTDFLKKEKRLGDDFTSEETGI
                     TEQLQKSKSEIQGVTHVDYSSRIQVVSETEDPLFFKLLTEYKKITGRGILINTSFNLR
                     GQPIVCSPDDACKCFLETDIDLLVINNFIASKNN"
     gene            complement(6565..7887)
                     /locus_tag="PK134_01435"
     CDS             complement(6565..7887)
                     /locus_tag="PK134_01435"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HRB37547.1"
                     /translation="MDNIKEKFKEKALPMAGTIKDIVKTHGNFVLGQYTVEQVYQGMK
                     GMIGMVTETSKLDANEGIRFRGYSIPELREKLPVSKEGPEPLPEGIFYLLLTGEMPLL
                     KDVIEVNNEWMKRATVPKHVFDMLDMLPDKTHPMTQFSMAIMALQTESVFARKYREGM
                     NKKDYWDPMYEDVMNLIARLPRIAAYIYRSTYKDGKHIEPDPKLDWAGNFAHMLGFEE
                     LDFRKLMRLYLTIHADHEGGNVSAHATHLVGSALSDPYLSLAAGMNGLAGPLHGLAAQ
                     EVARWIIELRDKYNGVPSREQLGEFIKTTLAEGKVVPGYGHAVLRKTDPRYTAQQDFA
                     KKYMADDELFKIVQLLYDVTPEILKATGKIKNPWPNVDAHSGILLMHYGLVEYDFYTV
                     LFGVSRAIGVLTSLLWDRALGMPIERPSSVTTQWIQEKIKSGDAVKAE"
     gene            complement(7919..8188)
                     /locus_tag="PK134_01440"
     CDS             complement(7919..8188)
                     /locus_tag="PK134_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37548.1"
                     /translation="MELEESDGEIKKERIRVRKRIRIKKKPDPKKKIKKYLRIFAWIV
                     VIAIFITTLIVLFSESDLRYENTKKPKSRVELNPSTKDFSVNFNL"
     gene            complement(8234..9523)
                     /gene="eno"
                     /locus_tag="PK134_01445"
     CDS             complement(8234..9523)
                     /gene="eno"
                     /locus_tag="PK134_01445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRB37549.1"
                     /translation="MGNIAEIRARQILDSRGNPTIEVDVYTDNGVVGRAAVPSGASTG
                     AHEAVELRDNDKKIYLGKSVMRAVNNVNAILNKELHGYPVFDQRSIDAAMIHIDGSPN
                     KATIGANAILGVSLACARAAASESGYPLYRYIGGVNARILPVPMMNILNGGSHADNSI
                     DFQEFMIMPVGAESFSESLRMGVEIFHNLKSVLKAKKYSTNVGDEGGFAPDIKSNEEA
                     ILTVIEAIEKAGYKPGDDVMIAMDAAASEFYDAENKVYHFKKSSGEKLTSSEMVSYWK
                     QWCDKYPIVSIEDGLAEDDWAGWKELTNVLGSKVQLVGDDLFVTNTERLARGIKENIA
                     NSILVKVNQIGTLTETINAVEMAKNNSYTSVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEMLGGAAEFPGKNFRNLI"
     gene            9632..10381
                     /locus_tag="PK134_01450"
     CDS             9632..10381
                     /locus_tag="PK134_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRB37550.1"
                     /translation="MKVLKIAFGLYAAIVFFITLLCVFPLYFLVFLFFSEKKSPVIAH
                     NLSRIWALILFKSFFIRYKIINGDFISDKGTYVFVANHRSMLDIPLYARACNNTFRFL
                     SKAELTKIPLLGYVIRNLYVTVKRGDKKDRQKSIEAMRKTLESDISIFICPEGTRNFT
                     DQMLLPFKDGAFRLAIATQTPLAVLTVINTDVLLSPESPLALSPGKLVAEWSKPIDTK
                     GMTDDDVESLKQQTAKLMQEILTRHLMSTQK"
     gene            complement(10404..12536)
                     /locus_tag="PK134_01455"
                     /pseudo
     CDS             complement(10404..12536)
                     /locus_tag="PK134_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682427.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(12722..16708)
                     /locus_tag="PK134_01460"
     CDS             complement(12722..16708)
                     /locus_tag="PK134_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37551.1"
                     /translation="MWWNLVDGANSKAEYEIPKGTGKTSLFAGSVWIGGYDATGNLKV
                     AAQTYRQSDANDYFTGPIGVQGGNMSTDVSICNQYDRIWSVTKQQVRDFVSTGVATRD
                     IREWPGNGDQSRGELQLLAPFEDVNGDGLYISDSGDYPRYLLSGEYPAPSDPNKLPCD
                     DYIFGDQTLWWVFNDIGNIKKETNSAAIGLEFRAQAFAFSTDDEINNMTFYKYQIINR
                     SNQAFDSTYFGWWVDPDLGDAFDDYVGCDVNLGLGFCYNGDPDDGSGTIDGYGINPPA
                     VGADFFQGPLADPNDGIDNDRDGVVDEPGEQIKMAYFIYYENDFNGVGGNPQVSDDYY
                     QFLSGSWKNGQRWTRDGAMGQTPPSPTVPVTNFMFPGTTDPAFPGSDWSMASANITPF
                     DRRFLQSAGKFTLQPGAVNYITTGMVWARTNNGGPLASVDLLKLSDNKAQGLFDVCFK
                     TLDGPDAPDVQITELNNKVILSLKNFNNDRIEKYSQLDPSIPTLVQPDEEQRKFKFQG
                     YLIFQLKNASINTSDIFDPSKTSSSSVMSGDNYRLISQVDIKDTVTNLVNYFWAPEIP
                     GYRPLTVAFNPDQLTGTNSGLKHSFVIDKDAFTNKPLVNHKAYYYVVVSYAYNNFKPF
                     EYIVPNSSNFTQQKPFKVGRNNIKVMSAIPHAPEVEHNGLVFNSEVGDGMKVTRLEGS
                     GNGGLLIDMTDASAAEAINAPYVVSNPVYEIGKGPLNVTVYDPLYVTEGHFEMKFTGV
                     SGTDKWSVKETTTNSVDTATFELQQPNSQILQTVDAGDASKVYEWGLTADISNAVIEA
                     GNTDAINNGLLDQSITFANADAAWLGGVADRDTSIADARDWIRSGTAPSDIGGADNNQ
                     VYEGLIGGTWAPYKLANKVDAQFGPRSSAHQDNQLTMSGPNGITSVDVVFTSDKSKWT
                     RAVVLEMGQPTSATIGNAPRFQKRRSASINKDGGNNPGGAVSTDPNDPNYIDNEGMGW
                     FPGYAINIETGERLNIAYGENSVFTTENSQDMQWNPTSTLLDGNGNPVFGGMHYIWVF
                     GSRTTQGARDIGKYDACDKFNSLIKSTSASERRDALANIMYVSIPLLNPGRSLSETDA
                     KVRIRVARQYRTFNTNFDAGSYINHSGTGTPLTRGVTYAVVSGTVTAAFNNNSITYSP
                     GQTITIPTDANTFPDASITSAGGVVLAPTVNGTNPYYKVDSEGKEPSFDNSDVAKSAL
                     KLVNVVPNPYYAYSSYDKSTNMMWVKITNLPAKCTVSIYTMNGVLVKRFNRNSGSSID
                     GTAIGLSQGTEAGSGNNDTSIDWDLTNEKNVPISSGVYLIHVKAPGLGERTLKWFGVM
                     RPISLDSY"
BASE COUNT         5057 a         3420 c         2923 g         5361 t
ORIGIN      
        1 ccatatcagc accttcaggt ggagtatcgc tattaaatat attgtggtca gatggcagca
       61 cttccacatc tgcacaagct acggcaggaa cagtaatgac cttaacagtt accgacaatg
      121 caggatgtaa tagagatact acattcagta tgttatcaac caacggcccg caaatttcaa
      181 gtattcaaac gacagacatc atttgttttg gagacagtag cggcagcgca acagtcaata
      241 ttacaggagg aacacctcca ataagcattc aatggatagg cactttaccg gttcaaaata
      301 ctgcaacggc tataaattta aaagccggaa cttatacctg tattgtaaca gacgtaaatg
      361 gttgttcagc acaaagtgtt gcaaatatca atcacctgaa tacattgata actccagtgc
      421 ttgctataac aaatgcaagt tgtggaaaca gcaacggaca gattgttgta actgcaaccg
      481 gtggtagtgg tacctatcag tacaaactta ataatggagt ctattctgca acaaatacat
      541 ttactaacct cgcagcagga tctgattcgg tttttgttaa agacaacaat ggctgtgaag
      601 tatttgttat ttcgactata agtaatatac aagggccggt aatatcagtt attgactcgg
      661 ctaatgtaag ctgctttggc agcaatgatg gtttcattat tttaaatgtt accggtgcag
      721 cacagcctat aacctattca tggtctgatg gaccaacaac tttaacaaga aataatcttg
      781 gaccaggcaa ctatacattt acagcgcagg acgcaaacaa ttgtatatcg gtaagaaact
      841 ttatcattac agaacctaca gccattacaa tcagtgaaat aattccaaat aaaaacggta
      901 tttacaatat aacttgcaac acacttgctg acggagaaat tcaggtcatt gcaaatggag
      961 gtgttccatt tacgtcaaca actgcttaca actatcaatg gtcaatcagt ggcgcttcag
     1021 gagctaatgt gataaatctt actgcaggac cttatactgt aactgtaaca gattctatgg
     1081 gatgttctgt tacaaaatca ttcctattaa ctgagcctcc tgccttaaat gcaggccctg
     1141 attttacaca atctatttgt ggtactgata caattgtact tacaggaaat atccctactt
     1201 atggatttgg aaaatggact gttcgtagtg gaggtggatt aattgctgaa ccggattcaa
     1261 tcacaacaca gattactgca attgcaaatg gggtaaatgt ttttgcatgg gtagtctatg
     1321 acaatttctg ttcagacact gcattatatg tcatcaacaa aaaagaaaaa attatagcag
     1381 atggtggcat tgatcaaaat gtgtgcgaca attttgtatt ccttgcagca gtaccaccac
     1441 cttcaggtac tggtgtatgg caaagcatca atggaaatag tactattcaa aatccatcat
     1501 cacctgcgac taaggttgaa aatttaagtc cgggtaaaaa tatttttgtt tggacaataa
     1561 caaatggttc atgtactgca tccgatacag taaagattgc cattcttcct ccggaagaat
     1621 gttatactga tgttgttatg ccaactggtt ttacaccgaa tgaggatggt tataacgata
     1681 tatacgttat cagaggatta gataatacag ataatacttt gaccattttt aacagatggg
     1741 gcaatttagt ttatgaaaag aaaaactata gtaacgactg ggatggtaaa tctaacaaca
     1801 aacaaatctt acctgatgga acttactatg ccattttacg gatacctgcc agaaacttag
     1861 tcttaaaaac atatattgat ttaagaagat aagcttatga aaaaattata ttcattaata
     1921 attacactct ttatgtcagg ctggttgttt ggccaacaag caccaatgac aacacaatat
     1981 atgtttaata cactgctgat aaatccggct tatgcaggaa gtaaaccatt caccagtgca
     2041 acaatgatgg ttagaaaaca atgggcagga tttaaaggat caccgacaac agaaactgcc
     2101 agtatccatg gtgcgttgga agataaaaac gttggcttag gcttgtatat aagtaatgat
     2161 catgcaggca ttacaaatcg cacagaactc tttggaagtt attcttatac tattgaatac
     2221 ggagaatcaa aacttgcatt cggtcttcag gcaggtttga gttatctgaa atccacacta
     2281 tctgatcttg tttataacga caacaacgac cctgtatatg ataagaatac tgtaaataac
     2341 gtgttgccaa attttggcgc aggtctatat tatcacaccg acctattcta tgcaggcatt
     2401 tctgcaccat tacttttaag ttacgatcca ctaaaaactt tgtcagttga aatgaccgat
     2461 gtacatcata tcagaagaca ctattttcta acaaccggtt atgttttttc attaagtgag
     2521 caggtgaaat taaaaccttc tgttctgcta aaatatgtta atggtgcccc attacagtat
     2581 gacattaatg caaatctgct tttgaacaat atcatctgga taggaacttc ataccgctcc
     2641 aatgatgcac ttgcaattat tggagaatat cagctatcta aaaaactacg cattggttat
     2701 tcctttgatt attcgcttac cattctcaga aaccattcgg caggatcgca tgaatttatg
     2761 cttggatacg actttggtta taacgtacct aaaattaaaa caccgcgtta tttttaatta
     2821 tgattagaaa acgcaagccc tatttattat ttgtagttct gatattcaca tcatgcttat
     2881 ctgtcaaaca aagaacagca gacacctatt atgagcagaa cttatatgac aaagcgattc
     2941 cgtattatca ggaagttgca aacaaaaagt ttaatttaga tatcctcatt aaacttgcag
     3001 attgttatcg caatgtaaag aactatccat tatcagaaca gatttattcg aagatagtaa
     3061 cttcaaatag tgacccgata tatcaatttt attatgctga agcattaatg caaaatggca
     3121 aatatgttga agcccgaaaa tggttaaaca aatttcttgt tttaaatcgt gcagacagca
     3181 gagcgttaaa cttattagca tcatgcgatt ctattgaaat gctatacaag gactcattgc
     3241 tctttgatgt tacaagattg ccttttaacg gaattgatga aagcaatttc tcacccgaat
     3301 tttttcattc cggaattgtt tatgtaagta acagaaatat tgatgcaaaa agttataaga
     3361 caaatccggt aattgataat tacaatttat tttatgcaca acaaactgaa ttaggtcact
     3421 ggatggatcc ggttccttta cggggcacaa ttaattctat ttttaatgaa gggccatgca
     3481 cattcaacac tgaatttact aaagcttact ttacacgcaa tgccaatgaa gggaaaaaaa
     3541 ttattactaa tcagcaaagt gaaaatgttt tagaaatttt tgaagctaca accaaagatg
     3601 gtgaatggaa tatttcaggg cctgttagtt tcaacaataa caattacagt gtagggcatc
     3661 catctatttc acccagtggc aacacaatgg tctttgtttc tgatatgcca tggggttatg
     3721 ggggtacgga tatttataaa gtacaatggg ttaataatac ttggagtaaa ccggttagta
     3781 ttggcaagac aattaattca agtggaaatg aaatgtttcc ttacttactg actgattcaa
     3841 tactttactt ttcaagcaac ggtatgattg gtgttggagg tctggacatt taccgaacag
     3901 aattaactgc tgatggatgg actgtaccag aaaacctagg ctatccgatt aacagtccaa
     3961 gagacgattt tggtttgatt tgcgataatg aagaattaag tggttatttc agtagtaaca
     4021 ggcaacaagg aaaagacaat attttttcat ttaaaagaaa tcctccggtt ttaacgttaa
     4081 aaggcaaatt gattgacaag tcaacaaaca agtctctaaa aaacatcaat attacgttaa
     4141 ctgcttcatc aattgatact gttttaacaa cagatagcga aggcaatttt tctgcgcaat
     4201 taaaaaacaa taaagaatac aaacttcgtg ctgaggataa aagttatttt acagcatcag
     4261 aaaagatatc tacgtttggt tatagaaaat cagcaacact gaataaggat ttaactttgg
     4321 aaaaagtgat tttcaacaag ccagttatat ggagaggcat tgcatttgaa aaaggaaaat
     4381 cagatttaac accgtcaact aaaacagaat taaataaact gtttaacatt ttattagaga
     4441 ataaaaatat aaaaattgaa ttgagctgtc acacagactc aagaggcata gaacgtgata
     4501 acctgatatt atcacaggca agagcagata aagctgctgc atatcttatc aataaaggaa
     4561 tctcaaaaga cagaattatt tcagttggat ttggatccca acgattatta aacaattgtg
     4621 taaggggtgt attatgtctt gaagaagacc atcaggttaa tatcagaact gaaataagat
     4681 acagtcctta tcagcgataa gacttaatag acagaacata tttttctgca aagatggcca
     4741 tcatattagg catttccgca ttttatcacg actcggcagc aactattgct attgatggta
     4801 aaataatctc agcagtacag gaagaacgct tttcaagaat taaaaatgat gcctcttttc
     4861 ctgtcaatgc aataaaattt tgcttgctgc attccggttt aaagttaaat caaattgatc
     4921 atattgtata ttacgaaaaa ccttttttga agttagagag gttattggaa acttttcttg
     4981 aaacagcacc taaaggcttg caacaattta cacaggctgc accaatatgg ataaaagaaa
     5041 aagttctgat aaaaaataaa atcagagaaa gtttgcattt gatagatgag aattattcag
     5101 caaaagaaat tctattctgc gagcatcatc tatctcatgc tgcaagtgct ttctttacct
     5161 ctccttttcc ggatgcagca ataataacta cagatggtgt tggagaatgg tgcactactt
     5221 caattatgaa tggcaaacta aattctatta cctctataaa agaacttaac tttccacatt
     5281 caataggatt attatatagt gcatttacac agttccttgg ttttaaagtt aattcaggag
     5341 aatataaagt aatgggcttg gcaccttatg ggaataggaa tagtgcacaa tataaattat
     5401 acaagaataa aatcagcgaa ttagttgacc tgaagacaga cggttctgta tttctgaaca
     5461 tgaaatattt taattacctg acagggttaa acatgataaa tgaaaacctt tggtctgagc
     5521 tttttgaaat aaaaccacgc actgctgaat cagaaataaa tttaaatcat tgcaacttag
     5581 cattggcaat tcaaacgatt actgaaaatg taattttaaa tttagcaaaa gaagctaaac
     5641 aaattacaaa ttcagacaat ttatgtttgg caggtggagt ggcattaaat tgtgttgcag
     5701 ttggcaaact caacaaacaa aatattttca aaaaaattta tgttcaacct gcctcaggtg
     5761 atgcaggtgg tgccataggc gctgcacttg cagtagagca catattttta aacaataaac
     5821 gtaaagttcc tgaaaaagat ttaatgcaag gttgtcgttt aggtccttct tacgacaatc
     5881 atactgttac acagactttg aaaaaacaca atttacactt tgaagaattt aaagaaggtg
     5941 agcttttaga acgtactgcc agacatcttg caaatgataa aactatagca tggtttcagg
     6001 ggcgaatgga atttggaccg cgtgcacttg gatcaaggag tatttttgca aaccctgcaa
     6061 caccgcaaat tcaacaaaca ttaaatcaaa aaattaaaaa gcgtgaatca ttcaggccct
     6121 ttgctcctgc aatgttactt gaagattact tagaatttta tgaaggaggt aatgaaaata
     6181 gttttatgtc tatgactgat ttccttaaaa aagaaaaaag acttggtgat gactttactt
     6241 cagaagaaac aggaataact gaacaattgc aaaaatctaa aagtgaaatt cagggagtaa
     6301 cacatgttga ttacagttca cgaattcagg tggtatctga aacagaagat cccttgtttt
     6361 ttaaacttct tacagaatat aaaaagataa caggaagagg aatactcatt aataccagtt
     6421 tcaatctaag gggacaacca attgtttgca gccctgatga tgcctgcaaa tgctttctcg
     6481 aaacagatat tgatttattg gtaataaata actttattgc cagtaaaaac aactaagcca
     6541 aattcgctct gaaataaaca gcagttattc tgcctttaca gcatcgccag atttaatttt
     6601 ctcctgaatc cattgtgttg ttacagaact tggcctttca ataggcatac ctaaagcacg
     6661 atcccataaa agtgaagtca gaacaccaat agctcttgat acaccaaaca aaacagtata
     6721 aaaatcatat tccaccaaac cataatgcat cagcaagatg cctgaatgtg catcaacgtt
     6781 aggccatgga tttttaatct taccggtagc ttttaaaatt tccggtgtaa catcatataa
     6841 taattgaact attttaaaca gttcatcatc tgccatatat ttctttgcaa aatcctgctg
     6901 tgccgtataa cggggatcgg ttttacgcag tacggcatgt ccgtaaccgg gaacaacttt
     6961 tccttctgct aatgttgttt taataaactc acctaactgc tcgcgcgatg gaacaccatt
     7021 atacttatca cgtaattcaa taatccaacg ggcaacctcc tgtgctgcta aaccatgaag
     7081 cggaccggca agtccgttca tacctgctgc caatgaaaga tatggatcac ttaatgcaga
     7141 gccaacaaga tgtgttgcat gtgctgaaac atttccaccc tcatgatcgg catgaatggt
     7201 aagatataat cgcatcaact tcctgaaatc aagttcttca aatcctagca tgtgagcaaa
     7261 atttcctgcc cagtctagtt taggatcagg ctcaatgtgt ttgccatctt tataagttga
     7321 acgatagatg taagcagcaa ttcgcggaag acgtgcaata agattcatta catcttcgta
     7381 cataggatcc caataatcct tcttattcat tccttcgcga tacttacgtg caaaaacaga
     7441 ctccgtttgc aaagccataa ttgccattga aaattgcgtc attggatgtg tcttatcagg
     7501 caacatatcc aacatgtcaa aaacatgttt aggcacggtt gctcttttca tccattcatt
     7561 attcacttca ataacatcct tcagtaatgg catttcgcca gtgagcaaga ggtaaaaaat
     7621 tccttctggc aatggctcag gaccttcttt agataccggc agtttttcac gaagttcagg
     7681 aatagaatat cctctgaaac gaattccttc attagcatca agtttactgg tttcagttac
     7741 catgcctatc attcctttca ttccctgata aacctgctct actgtatatt gacccaaaac
     7801 aaaattgccg tgggttttta caatgtcttt aattgtacct gccattggca aggctttctc
     7861 tttgaatttt tcttttatgt tgtccatact tgttgtataa ctgttactta aacgtttgtt
     7921 acaaattaaa gtttacagag aagtcctttg ttgaaggatt taactctact cttgatttag
     7981 gtttcttggt attttcataa cgaaggtctg attctgaaaa caacactatt aatgttgtaa
     8041 taaaaatggc aataactaca atccatgcaa aaatgcgcaa atacttttta atcttcttct
     8101 taggatcagg tttcttttta attctaattc gtttccgaac tctgattctt tcttttttta
     8161 tttcgccatc tgactcttcc aattccattt tcaagttgtt aattcagatt ctaaagatac
     8221 agcccctttt ggattaaatc aaattcctga aattttttcc gggaaattct gcagctccac
     8281 ccagcatttc ttcaattcgc aggagttggt tgtacttggc aatcctgtca gaacgcgatg
     8341 ccgaaccggt ttttatctgt cctgtattta atgccactgc caaatcagca atagttgtat
     8401 cttcagtttc gcctgaacgg tggctcatca ctgatgtata gctgttgttc ttcgccattt
     8461 caacagcatt gattgtttct gttaaagttc caatctgatt cacttttacc aatattgagt
     8521 tggcaatatt ttcttttatt cctcttgcaa gcctttcagt gtttgtcaca aacaaatcgt
     8581 cacctactaa ctgcacttta ctgcctaata cattggttaa ttctttccat cctgcccaat
     8641 cgtcttctgc aagtccatct tcaatactaa caatcgggta tttatcgcac cattgcttcc
     8701 agtagctaac catttctgaa gatgtcagtt tttcaccact tgacttttta aaatgataca
     8761 ctttattttc cgcatcataa aattcagaag cagcagcatc cattgcaatc ataacatcat
     8821 caccgggttt gtagcctgcc ttttctattg cttctatcac tgttaatatt gcttcttcat
     8881 tagatttaat gtcaggtgca aaaccgcctt catcaccaac gttggtagaa tatttcttag
     8941 cttttaaaac actcttcaga ttgtgaaata tttcaacacc cattcgtagt gattcagaaa
     9001 aactttctgc tccaacaggc ataatcataa attcctgaaa gtctatactg ttatcagcat
     9061 gagatcctcc atttaagatg ttcatcattg gcacaggcaa tatacgtgca ttaacaccac
     9121 cgatataacg atatagcgga tagccggact cacttgcagc tgctcttgca catgctaatg
     9181 aaacacccaa aatagcattg gcacctattg tagctttatt aggtgaacca tctatatgaa
     9241 tcattgccgc atcaatactg cgctgatcaa aaacaggata accatgcagt tctttattca
     9301 aaatggcatt tacattattt acagctctca tcacactttt gccaagataa atcttcttat
     9361 cattatcacg caactcaacg gcttcgtgtg ctccggttga tgcacctgaa ggtacagcag
     9421 ccctgcccac tacaccattg tctgtatata catctacttc aatagttgga tttcctcttg
     9481 agtcgagtat ttgtcttgct ctgatttcag caatattgcc cataatattt tacactttat
     9541 aaaacggctg caaagttaac gtaatgtgcc gtaaatttat ttaatttagc gaaataaatt
     9601 tcaaccacat ctataacact aatagcgcta tatgaaagtg cttaaaattg catttggatt
     9661 atatgcagca attgtatttt ttatcacttt gctttgtgtt ttccctctat attttttagt
     9721 atttttattt ttcagtgaaa agaaatctcc tgttatagca cataaccttt ctcgtatttg
     9781 ggcattgata ttgttcaaaa gtttttttat tcgctacaag ataatcaatg gtgattttat
     9841 ttcggataaa ggaacctatg tatttgttgc caaccatcgc tctatgctgg atattccact
     9901 ctatgcgcgt gcatgtaaca ataccttcag atttttatct aaagccgaac tgactaaaat
     9961 cccgttactg ggttatgtaa tccgaaatct gtatgtcact gtaaaacgag gtgataaaaa
    10021 agacagacaa aaaagtattg aggcaatgcg taaaacactt gagtcggata tttcaatatt
    10081 tatttgtcct gaaggcactc gtaattttac agatcagatg ttgctgccat ttaaagacgg
    10141 tgcatttcgt ctggctattg caacacaaac acctttggca gtactcacag taataaatac
    10201 agatgtattg ttgtcacctg aatcaccatt agctttgagt ccgggtaaac tggtagcaga
    10261 atggagtaaa cctattgaca ccaaaggaat gactgatgat gatgttgaat cactaaaaca
    10321 acagacagca aaactgatgc aagaaatatt gacacgtcat ttaatgtcaa cacaaaaata
    10381 atttttgttt ttgttgatta attttaactg cttagtgctt ccactttcac atattcatcg
    10441 tgtaatttct tttgtaagtc tccgggcaca ggagcaaatt cagcaaatga tgtttttaac
    10501 ttagcctttc cttgtgtgat ggattttaat gcagtgctgt aaccatcaag ttcaagcaaa
    10561 ggagtttttg cacgaattac ctgtcttcca tttcgagagt ccatacccat aatgatactc
    10621 cttctcgact gcaggtcact cattacatcg cccatcatat catcaggcat ttctacttca
    10681 acatcataaa taggttctaa aagttgtggc tgtgcattgt gaaatgcttc ccgaaatgcc
    10741 atcatgcctg caatcttaaa tgatatatca tttgaatcaa caggatgcat tttaccatca
    10801 aacacactta ctcttatatc acgcacaaac gatcctgtta acggaccttc ctgcattttt
    10861 tccattacgc ctttaagaac tgaaggtaaa aagcgtgtat caatagcacc tcctgtaata
    10921 caattattaa aaactaattt tccaccccag ggtaatgcat gctcttcttt atcacgaagt
    10981 ggatattctt tcatatcagg cataccatca tagtaaggtt caatttttat gtgcacttca
    11041 ccaaattgac ctgctccacc tgattgtttt ttatgtctgt aagttgcaac tgcaggtttt
    11101 tgaattgttt cgcggaacga tatgcgtggt tttataaact caacctgcat tttatataca
    11161 ttttcaatcc gccattttgt tactgcaaga tgtaaatcgc cttgcgcatg taaaataacc
    11221 tgcttgagtt ctttagaata ttctactact aatgtaggat cttcaagatg taattcactc
    11281 agcacctcgc ccaatttttc ttcatcgctt ttgttttttg caatgagtgc tactctggat
    11341 tttggttcag gaaattgtat tggttcaata ttaatagtta taccttttcc gcaaagcgta
    11401 tggtttgtaa gtgtattctt caatttaaga gtaaatccta aatagcctta cttaagttta
    11461 tcagctgaat ttctactctt tccatccatg ataaacaact gggtcaatct ttcgcttgta
    11521 tttgttttgt gattcattaa atccgtccct gcacttacct ctccagaaag aactttaaaa
    11581 aaggtcaact tacccaaatg tggctcaatt atagttttat aaaaaaacaa aactgttggt
    11641 cctgttggat tacatggaac taaggtttta tcttcagcaa tttctgccgg catgtcaatt
    11701 gccgaaggtg caacattatc aataaacccc atcagtcgtc cgctgcccat atctttcttt
    11761 gccgataagc agaaaacagg aaaaatctga tgtgccatca ttcctgcttt caatccttgt
    11821 ttcatttcat cttcgtcaag agatcctcgc tcaaagtata actccatcag tttttcatca
    11881 ttctcagcag ctttctccac caattcatta tgcagctttt ttgcatactc catttcttct
    11941 ggtgggatgg caagcttctc aggtttgccg cctgtggcag gaaaacgata catggtcatt
    12001 ttcaataaat ctattatcgc attaaatcct atcccttgat ttaacggata ttgcataacg
    12061 gtaatggcat tgccgaactt ctgttttgct ttttctacag ttgcattaaa attggcatgt
    12121 tcaaggtcaa gctgattgac tgcaaatatg gttggcttcc ggtatttatc cacgtaatcc
    12181 caaataacct cactgcttgc ttcaacacca tgttgcgcat ttaataacat gacggcagta
    12241 tcacacactc tcacagcaga tattacttct cctataaaat catctattcc cggtgtatca
    12301 attatattaa ttttataatc acgccattcg gtgtgcatgg ttgtttcata aacagaactc
    12361 tgtcgttcat gttcaatttc atgataatct gaaactgtgt ttttttcttc tacagaacct
    12421 cgtttggtta ttatgccggc ttcaaagagc atggtttctg caagcgttgt cttaccgctt
    12481 tttgctgatc cgacaagtgc aatattcttt atgtgttttt cgtcataagt tctcatacaa
    12541 cctttgttta aaatgaggta tgaaaattaa ggaacaaaaa ctgaacttgc aaataaatta
    12601 agctcctgtc atcaaaattt aatggggaga agtcacatta gtgataaatg atctattgca
    12661 tgttgcttaa aaacctgcaa tgcttgaatt ttaataaaca attacagata aataaattcg
    12721 attaataact atctaaacta atcggacgca ttacaccaaa ccacttaagt gttcgctcac
    12781 ccaagccagg tgcttttaca tgaatcagat aaacgccact gctgataggt acattcttct
    12841 cattcgtcaa gtcccagtca attgatgtgt cgttgttgcc actgccggcc tctgtgccct
    12901 gactcaaccc tatggctgtg ccatctattg atgaacctga attgcggttg aaacgtttta
    12961 ccaatacgcc attcattgtg tatattgaca ctgtacactt tgcaggcagg ttcgtaatct
    13021 ttacccacat catgttcgtt gacttatcat acgatgaata ggcataatat ggattgggga
    13081 ctacattcac cagcttcaac gcactcttgg ctacatctga attatcaaaa cttggctcct
    13141 tgccttctga gtcaaccttg taatatggat tcgtgccatt taccgttggt gccaatacca
    13201 cgccacctgc cgatgttata gatgcatcag ggaatgtgtt tgcatccgtg ggtatggtta
    13261 tcgtctgtcc cggtgaatat gttatgctgt tgttgttaaa cgctgctgtt actgtgccac
    13321 tcactactgc atacgttaca cctcttgtca atggtgtgcc cgtaccgctg tggttgatgt
    13381 agctgcctgc atcaaagttc gtgttgaacg ttctgtactg acgtgctacc cggattctta
    13441 ccttggcatc tgtctctgat aaacttctgc caggattcaa caagggtatg ctcacataca
    13501 taatgttcgc caatgcatct cttcgctctg aagctgatgt gctcttgatt aaactgttga
    13561 atttatcaca tgcatcgtac ttacctatat ctcttgcgcc ctgtgtggtt cttgaaccaa
    13621 atacccagat gtagtgcatt cctccaaaca ccggattgcc attgccatcc aataatgttg
    13681 atgttggatt ccactgcata tcctgactgt tttctgtggt aaacactgag ttctcgccat
    13741 aggctatgtt cagacgctcg cctgtctcta tattaattgc atagcccggg aaccagccca
    13801 tgccttcatt gtctatatag ttcggatcat tcggatctgt tgatactgcg cctccgggat
    13861 tgtttccgcc atctttgttt attgatgccg atctgcgttt ctgaaaacgt ggcgcattgc
    13921 ctatcgttgc actggttggc tgtcccattt ctaatactac cgctcttgtc cacttcgact
    13981 tatcactcgt aaacactacg tcaacacttg tgattccatt cggaccactc atagttaact
    14041 gattgtcctg atgtgctgat gagcgtggac caaattgtgc atctactttg tttgccaatt
    14101 tatacggtgc ccaggtgccg cctatcaagc cttcgtaaac ctgattgtta tccgctccgc
    14161 ctatatcgct tggggctgta ccactgcgta tccagtctct ggcatctgca atacttgtgt
    14221 ctctgtcggc tactccgccc aaccatgctg cgtctgcatt agcaaacgtt atactctgat
    14281 ccaataatcc gttgttgatg gcatccgtat tgcctgcttc tatcactgca ttgctgatat
    14341 ctgccgttaa accccactca tataccttgc ttgcatcgcc tgcatctacc gtttgcagta
    14401 tctggctgtt gggctgctgc aattcaaatg tagctgtatc tactgagttt gttgttgttt
    14461 cttttacact ccatttgtct gttcccgata ctcccgtaaa cttcatctca aaatggcctt
    14521 ctgttacata caatggatca tataccgtta cattcaacgg gcctttacct atttcataca
    14581 ccggatttga tactacatac ggtgcattga ttgcttctgc tgctgatgca tcggtcatgt
    14641 caatgagtaa tccgccattg cctgatcctt ccaatcgtgt taccttcatg ccatcgccta
    14701 cctctgagtt aaataccaag ccgttatgct ctacttcagg tgcatgaggt atggccgaca
    14761 ttacctttat gttgtttctg cctaccttaa atggtttctg ctgtgtgaag ttcgatgagt
    14821 ttggtactat gtattcaaag ggtttgaagt tgttgtatgc atagcttact actacataat
    14881 aatatgcctt atgatttacc aagggcttgt tggtaaaggc atctttgtct attacaaagc
    14941 tatgtttcaa tccactgttt gttcctgtta actgatcggg attaaatgcc actgtcaacg
    15001 gacggtagcc cggtatctca ggtgcccaga agtaattaac taagtttgtt actgtatctt
    15061 ttatgtcaac ctgacttatc aggcgatagt tgtcgccact cataacactt gatgaggagg
    15121 tcttggatgg atcaaatata tctgaagtat ttatgctcgc attctttaac tggaatatca
    15181 ggtatccctg aaacttaaac ttgcgttgct cttcatcagg ttgtaccaat gttgggatag
    15241 atggatctaa ctgtgaatat ttttctatcc tatcattatt gaaattcttt aatgaaagaa
    15301 taactttatt gtttagctct gttatctgta catccggtgc atcagggccg tccaatgtct
    15361 tgaaacatac atcaaacaag ccttgtgctt tgttatcgct aagctttaat aaatctactg
    15421 atgctaatgg accgccattg ttcgtacgtg cccacaccat acctgtggta atatagttta
    15481 ctgctcccgg ttgcaaagta aattttcccg ctgactgcaa aaaccttctg tcaaatggtg
    15541 taatgtttgc tgaagccatg ctccaatcac ttcccggaaa tgcagggtct gttgtacctg
    15601 gaaacatgaa attagtaact ggtacagttg gtgagggtgg tgtctgaccc atagctccat
    15661 ctcttgtcca gcgctgaccg tttttccatg acccggacaa aaattgataa taatcatccg
    15721 acacctgcgg attacctcct acaccattga aatcattttc atagtaaata aaataagcca
    15781 ttttaatctg ctcaccgggc tcatcaacta cgccatctct gtcattatct attccatcat
    15841 tagggtctgc taagggtcct tgaaaaaagt ctgcacccac tgcaggtgga tttattccat
    15901 aaccatctat ggttccgctt ccatcatcag ggtccccatt ataacaaaaa cctaatccca
    15961 aatttacatc acagcctaca taatcatcaa atgcatcacc caaatcaggg tcaacccacc
    16021 agccaaagta tgtgctgtcg aaggcctgat tgctgcggtt gatgatctga tacttataaa
    16081 atgtcatgtt gttaatctca tcgtccgtgc tgaaggcaaa tgcctgtgct ctgaactcaa
    16141 gacctattgc tgcactatta gtttccttct taatatttcc tatatcatta aatacccacc
    16201 acaaggtctg gtctccaaaa atataatcat cacatggcag tttatttgga tcgcttggtg
    16261 caggatattc tccactcaat aaatatcgtg gataatcgcc actatccgaa atatacaagc
    16321 catctccgtt tacatcttca aatggtgcca gcagttgcaa ctcgcctctt gactgatcgc
    16381 cattgcccgg ccactctctg atgtcgcgtg tggctactcc ggttgacaca aagtcgcgca
    16441 cctgctgctt ggtcactgac catattctgt catactgatt acagattgat acatctgtac
    16501 tcatgtttcc accctgaaca ccaattggac ccgtaaagta atcgttggca tcactttgtc
    16561 tgtatgtttg tgctgcaact tttaagtttc ctgtggcatc gtatccgcct atccataccg
    16621 agccggcaaa caacgatgtt ttgcctgtac cttttggtat ttcgtactct gcctttgagt
    16681 tagctccatc tactagattc caccacatat ctcccgcaga aaatattctc gctcttacat
    16741 tgttaatctg caaatctgtt t
//
LOCUS       DAORQT010000018        15766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_343_length_15766_cov_8.884794, whole genome shotgun sequence.
ACCESSION   DAORQT010000018 DAORQT010000000
VERSION     DAORQT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15766
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_343_length_15766_cov_8.884794"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PK134_01465"
     CDS             <1..1707
                     /locus_tag="PK134_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRB37552.1"
                     /translation="IDWLVLALTLLAIVFYGIYKSRHKKTLDSYLIANRELPWYNVCL
                     SVMATQASAITFLSAPGQAYSDGMRFVQFYFGLPLAMIFLSATFIPAFRKFNIYTAYE
                     FLERRFDHKTRKLTALLFLLQRGISTGISIYAPSIVLSTVLNIPIFYTTMIIGLLVII
                     YTFYGGTLAVSHSQVLQMTIIFSSIIIAGVIVLSLIGTDASLYESLKIAGIHNRMNII
                     DFKFDWNNRYNVWSGIIGGFFLQLSYFGTDQSQVGRYLTGKDSSASKLGLIVNGMVKI
                     PMQFVILFIGILVFSFYQFQPPPIFFNENIEKIAKESPMADNYNSLEHKFNDINQSNS
                     HLSKDYLKYLRSNDLRNAEITKAKILQTSAEANEVKQEAVKIIKTINPGADINDTNFV
                     FLHFVMHHFPKGFIGLIIAIIFLASMGSLAAGINSLTSTSVVDFYFRSSKKEHSDSKK
                     LYYSKIATLLWGIFCILCAFFAGNTGNLIEAVNILGSLFYGTILGIFISAIFVKHLDG
                     NSVFIAALITEAIVIAFWYFDVMAFLWLNLAGCLLVVLIALAIQFCTQNLNRMFNKTI
                     SK"
     gene            1753..2070
                     /locus_tag="PK134_01470"
     CDS             1753..2070
                     /locus_tag="PK134_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB37553.1"
                     /translation="MQEITVHELKQWLDEGKNFQLIDIREEYEYDACNLNGTLIPMGE
                     IIDRTEEIAKDKPVVIHCRSGSRSAAIYQVLSTQYGFTNLFNLKGGIKAYSQEIDPTL
                     NVI"
     gene            2080..2724
                     /locus_tag="PK134_01475"
     CDS             2080..2724
                     /locus_tag="PK134_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRB37554.1"
                     /translation="MEHLKFLIDFILHIDKHLFELVQRYDTWIYLILFLIVFAETGLV
                     VTPLLPGDSLLFAAGALAAPPDNPLNVFYLMIIFFFAAFGGDNTNYFIGNYIGPKVFE
                     KNYRFLKKDYLNKTQEFYEKHGGKAVIFARFVPIVRTFAPFVAGVGSMTYNRFIGFSV
                     LASILWINICVWAGYFFGRLEIVKNNFSLVVIAIIGISLVPILIGIIKQKFSSK"
     gene            2721..3443
                     /locus_tag="PK134_01480"
     CDS             2721..3443
                     /locus_tag="PK134_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRB37555.1"
                     /translation="MRNFGLIGFPLSHSFSQKFFTDKFKKLNLKCVYNNYELSNLNNL
                     RELISTNQLTGLNVTSPYKTGVIPYLDRLEETAEAIGSINTILIKDKKLIGYNTDIIG
                     FKISLERLIGNQKPPTLILGTGGSAKTVAFVLKQKNIPFQFVSRKKKEGAISYSQSDD
                     FLSQNILIINCTPIGRFPDFGKKPPINYTKLTDKHFLFDLNYNPEITAFMTEGINKGC
                     KVCNGYDMLIEQAEASWVIWNT"
     gene            3560..4108
                     /locus_tag="PK134_01485"
     CDS             3560..4108
                     /locus_tag="PK134_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37556.1"
                     /translation="MNNILKDNKTKFITLSILVVAISRLLPHPYNFTPIGAIALFGGT
                     FLKDKKMAFGLPLAALLVSDLLLQLIKPGSAFYEGWFWVYLSFILITPLGFVLRNKLK
                     VQNVVAASLMGSIIFFVLTNFGVWATGLMYPLTGAGLMSCYVSAVPFFGGTIMGDLFF
                     NALLFGVFAFAKWRMPVLVKEA"
     gene            4143..5504
                     /locus_tag="PK134_01490"
     CDS             4143..5504
                     /locus_tag="PK134_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRB37557.1"
                     /translation="MYFNNILETIGNTPLVKLNSVTQGVQATVLAKIETVNPGNSIKD
                     RMALKMIEDAEKSGKLKPGGTIIEGTSGNTGMGLAIAAIIKGYKCIFTTTDKQSKEKV
                     DALRAFGAEVIVCPTNVEPEDPRSYYSVAARLSKEIPNAFYPNQYDNLSNRQAHYEQT
                     GPEIWEQTEGKITHLVVGAGTGGTITGTGRYLKEKNPKIKVWAIDTYGSALKKYHETG
                     ILDKNEIYPYVTEGIGEDFIPQNYDFTVIDHFEKVTDKDAALMTRDLVRKEGIWVGNS
                     AGSAVAGLLQMKEQLKPTDVVVIIFHDHGSRYIAKIFNDDWMRSKGYFDKKGMTASDI
                     IGSKKDVNLTTVEATLPIGDTLKIIKTNNFSQIPITREGRIVGNITENHIYSSLLKSP
                     ELKNKPVSDIMQEAMPFIDINTSIDTLSDLLSKNNQAVIVKDFKADRNYIVTRQDVVD
                     ALA"
     gene            5524..6297
                     /locus_tag="PK134_01495"
     CDS             5524..6297
                     /locus_tag="PK134_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRB37558.1"
                     /translation="MSFGICEMSLVPMRREPSHRSELVSQILFGEHYEVVESQKEWFK
                     IRTSHDGYEGFIALNQHTEISYNDFQFLQKNGAFVCFDLVQILLHDNKIQTVILGSAL
                     PFFENQHCRINTRTYRFDGNAKFPQKAATSALLIENAYMYLNAPYLWGGRSPFGIDCS
                     GFTQLVYKLSGYSLLRDAALQAEQGNIVNLVDEAMPGDLAFFDNEEGNIIHVGILTAR
                     DTIIHASGKVRIDAFDQVGIYNQETKRYTHKLRLIKRIF"
     gene            6306..6560
                     /locus_tag="PK134_01500"
     CDS             6306..6560
                     /locus_tag="PK134_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37559.1"
                     /translation="MNNPQLKASENFPATVNELYDEEGELVLILYTHELVDLPEGLWL
                     STEGYYEAVIKKTEIQIEDALFCISELNSISGMQYELTLS"
     gene            complement(6557..7627)
                     /locus_tag="PK134_01505"
     CDS             complement(6557..7627)
                     /locus_tag="PK134_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB37560.1"
                     /translation="MSKTVIVAPLNWGLGHATRSKTVIDNLLAAGCKVIIASDGNSLS
                     VLKRQFPVLQAEELPSYSPVYPLNGNMVWAMLKQTPKMLLNISNEQSAIKKIASKYNA
                     EIIISDNRYGCFTNFTKNIFLSHQLMIQSPESLLFLRPSINRLHAKYINRFHECWIPD
                     VEHGFKLSGILSENKFIKIPKYNCGLLSSFQSSNLSNDKKYDIVAILSGPEPQRTIFE
                     GKIIEQLEKLHCSALLIRGKISDPNEIPDRGALQFKNYMPADEIAKFINTDTLVICRS
                     GYSTIMDIAQLGVRALLVPTPGQTEQEYLASYLEENKIFMMQTQSNIDIQNAIEKKLE
                     YAAIRIEKNILLQQNLNRILLS"
     gene            complement(7655..9943)
                     /locus_tag="PK134_01510"
     CDS             complement(7655..9943)
                     /locus_tag="PK134_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB37561.1"
                     /translation="MMQKWIKVLSIISLLNVNILFAQKVGLVLSGGGARGFAHIGVIK
                     ALEENGIPIDFITGTSAGALVGGMYVSGTSPQQMQNIVLSDDFKNWATGTLSDELIYF
                     FKERERDASWATVKLSYDSTLRMNLNGALVNSGQIDFALLENHSAASAGADYDFNNLF
                     VPFRCVASDIKNKRPVIFNCGDLSQAIRASIAYPLYLSPMTADNQLYYDGGIYNNFPS
                     DVMLNEFDPDIMIGVSVGGGPDIPKEDNMFSQLRTMIIQSQHVVLPGADDILIQPDVT
                     NIGVFDFYNFQALIDSGYKATMARMDDIKQAIARRISPEELTQKRDNFISKLHPLIVG
                     DIVVTGVSPEKTKYIKSILNPRGSNMLLRQLKPRYFKLLADENIRYAFPKLVYDPKTE
                     VYNLEIYIKPETDLRVDIGGNFSSRPISEGFIGFRYNILGPYSLSIHANTYFGKLYNS
                     GQLKLRLDIPGRPDFFIEPFTTLNRMDYFKSSSFFKDDIKPAYLLVTDQSYGLNIGMP
                     ARNKGALTVGGNYFNIKDRYYQTRSFSVNDTADFTRFEGLSCVVGFERNTLNRKMYAS
                     KGSYLAIRTRFVSGNEVTNPGSLQDNAPTIREWHQWWQVKMIYDNYFFRAGRYKLGFY
                     AETNFSGQPFFSNYTATILQMPGFYPTQESRTLFLDNFHAPNYLGIGVRNVFSLRSNI
                     DFRLEGYAFQPFQAVVKNDNEKTVFGDALSARYFIASFSGIFHSPVGPVAMTLNYYDR
                     KTKSFTVLFHIGYILFNKHALD"
     gene            complement(9956..10762)
                     /locus_tag="PK134_01515"
     CDS             complement(9956..10762)
                     /locus_tag="PK134_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRB37562.1"
                     /translation="MLKFIDQTGFEIKLETPPQRIVSLVPSQTELLADLGLQDRIVGI
                     TKYCIYPPELFLSVPRVGGTKSVNHELINQLKPDIIIANKEENNREAIEQLRKNFPVW
                     LSDVSNLHEALDMIYNIGVMTDCQSRASEINSQIAEKFAALTIKNKKHTAKRALYFIW
                     RNPYMCVGGDTFISDMMNMAGYDNLMYYHKRYPELSEKKMAELNPEIILLSSEPYPFK
                     ARHIEEFKTICPKAEIKLVDGEFFAWYGSRMIKAADYFHQLCDSNSNVSE"
     gene            complement(10829..11305)
                     /locus_tag="PK134_01520"
     CDS             complement(10829..11305)
                     /locus_tag="PK134_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37563.1"
                     /translation="MRKLFMSSAICFAILLSTMVSFSQQTEKKSTESGFFVRNGLVYY
                     IKGNTVNFLESDQTLDNGATINQRGMIKYPDGKTAQLQQGEILTFNNVLVPKAVEEHF
                     ELKDGKLQIVTNGIFTPVQKAMKLETGVTIDEDGHTSSGITLMPKQKMNMMGELIK"
     gene            complement(11361..11657)
                     /locus_tag="PK134_01525"
     CDS             complement(11361..11657)
                     /locus_tag="PK134_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37564.1"
                     /translation="MKKLVKRLSALGAMLIISTVAFSQSTTTQETEQTTPPPTETTES
                     TEDSTAATEAVPDSTAVAPEGDPAAGAANEKPATRHKLYSGKQRGNNVIYDPKK"
     gene            complement(11909..12757)
                     /locus_tag="PK134_01530"
     CDS             complement(11909..12757)
                     /locus_tag="PK134_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRB37565.1"
                     /translation="MQKFTSSLMALIIATTTTSMAQLKTPQPSPTQSLTQAFGLGEIK
                     IEYSRPSVKGRTIFGDLVPYGKIWRTGANQSTKVTFGADTKVNGNDIAAGTYALYTIP
                     NTTDWEIIFYKDLTLGGNVGDYKQTDEMMRFKVKPVATADKMETFTININNIAPTSCS
                     IDLLWDKTKVSIPVTTNIDENVMKQIDKAMGDNKPYYQAANYYYENDKDLNKAREWIN
                     KAVENNPKAYWVLHTKAKIEYKLKNYTEATKAAEQSLALAKESSDNSYIKMNEKLIAD
                     VKKMGK"
     gene            complement(12863..13714)
                     /locus_tag="PK134_01535"
     CDS             complement(12863..13714)
                     /locus_tag="PK134_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRB37566.1"
                     /translation="MKWMGRRESDNVEDRRRFSGGKIALGGGLVGIIVIVINLLMGGD
                     PNQLLQQMQQQATTQTTTTESSAADDEMASFIKVALADNEDIWKKIFQENELEYREPK
                     LVLFRDATQSGCGNASSAVGPFYCPADEKVYIDLSFFDELKSRFGAEGGDFAVAYVLA
                     HEVGHHVQHLLGTSDKVHQMQEGLSETEANKLSVALELQADFYAGVWANQNQKLNNVL
                     EDGDIEEALSAASAVGDDKIQKKAQGYVVPDAFTHGTSEQRMYWFKKGYQTGKLQEGN
                     TFKELNI"
     gene            13799..14608
                     /locus_tag="PK134_01540"
     CDS             13799..14608
                     /locus_tag="PK134_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRB37567.1"
                     /translation="MLHNNLNMQNALKFILAYLFFTGNVSAQEILPNYMTQKEMLQMP
                     SYLQQLSNQLKNNQIAAPPSSPVRTIAEWEELQGILIGWTSYPSILREIVRAAKTECR
                     VYVVTNNEQSVINYLNSGNVDTINVSFVNTPFNSVWSRDYGPWSAYTNDVDTLVTIDW
                     IYNRPRPDDDNVPVAIAGLLNTPLYQTTMSPYSLVHTGGNFMCDGFGTGFSSNLIVNE
                     NPSLTISQIDTIMKQFMGINRYIKMPTLPYDAIHHIDMHMKLLNEETILMG"
     assembly_gap    14627..14660
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14661..15539
                     /locus_tag="PK134_01545"
     CDS             <14661..15539
                     /locus_tag="PK134_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRB37568.1"
                     /translation="SVFGTPYKIIRIPMPADNGAYPNTNGDYFTYTNSSFINNTIIVP
                     TYNIPQDSTALRIYRDALPGYNVIGINSLQSIGAIGALHCITKDLGTNDPLLISHQQL
                     SNTYDTINPYHVEAFIKHRSGIQSATLYYRTDTLQPYNTVSMNAISGMSDYFETYIPA
                     QSGGTRIYYYIEAYSNTSKHQTRPMPAPQGYFYFDVLQTTGITEVLNQLSVGQIFPNP
                     ASAVTCLPYYSRIGTLLQIKISDVTGKMVYESQNTTKSNGQNFAFFDVSQFKPGVYFV
                     KSETNSSINIQKLIVK"
     gene            complement(15578..>15766)
                     /locus_tag="PK134_01550"
     CDS             complement(15578..>15766)
                     /locus_tag="PK134_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37569.1"
                     /translation="VLHNSEISSLKISDINGRIVKEMYNLKDGSLDLTNLKDGVYNLQ
                     FFDKSKNNIATRIIVVKR"
BASE COUNT         5027 a         2752 c         2695 g         5258 t
ORIGIN      
        1 atagattggc tggttttggc gctaactctt ctggcaattg ttttctatgg tatttataag
       61 agtcgtcata agaaaacatt agatagttat ttaattgcca acagagaatt accttggtac
      121 aatgtctgtt taagtgtgat ggcaacacaa gccagtgcca taacattttt aagtgcacct
      181 gggcaagcat acagtgatgg aatgcgtttt gttcagttct atttcgggct tcctcttgcc
      241 atgatttttc tgagtgcgac attcatacct gcattcagaa aatttaatat ttatacagca
      301 tatgaatttt tagagagacg ttttgatcat aagacaagga aattaacagc actacttttt
      361 ttattgcaga gagggatttc tacaggaatt tctatttatg ctccttcaat tgtattatct
      421 acggtactta atattccaat tttttatacc acaatgataa ttggactatt agttataatt
      481 tacacttttt atggagggac tcttgctgta tcacattctc aggtgttgca gatgacaatt
      541 attttttcaa gtattattat cgcaggtgtc attgttcttt ctttaattgg aacagatgct
      601 tcactgtatg aaagtttgaa aattgccgga atacacaaca gaatgaatat tatagatttc
      661 aaatttgatt ggaacaaccg atacaatgtt tggagcggca tcattggtgg cttttttcta
      721 caactatcct attttggcac agaccaatcg caggttgggc gttatttaac gggcaaagat
      781 tcatcagcta gtaagctagg tttaatagta aatgggatgg ttaaaatacc tatgcaattc
      841 gtgattttat ttattggcat tcttgtgttt tcattctatc agttccaacc tcctcccatt
      901 ttctttaatg aaaatataga aaagattgcc aaagaaagtc cgatggctga taactacaat
      961 agtttagaac ataaattcaa tgatattaac caaagcaatt ctcatctatc aaaagactat
     1021 ttaaaatatt tacgcagcaa tgatcttaga aatgctgaaa tcactaaagc caagatactt
     1081 caaacttcgg ctgaagccaa tgaagtaaag caagaagctg taaaaataat caagactatt
     1141 aatccaggag ctgatataaa tgataccaat tttgtttttc tgcattttgt catgcaccat
     1201 tttcctaaag ggtttatagg attgattata gctattattt ttcttgcatc aatggggtct
     1261 ttagcagcag gtattaattc acttacttca acatcggtag tagatttcta tttcaggtca
     1321 tcaaaaaaag agcatagcga cagtaagaaa ctttactatt ctaaaattgc aacattactt
     1381 tggggaattt tttgcatact ctgtgcgttc tttgccggca atacaggaaa tctgattgaa
     1441 gcagtaaata tattggggtc attattctac ggcactatat tgggcatttt catttctgca
     1501 atttttgtaa aacaccttga tggcaattcc gtttttattg cagctttaat tactgaagct
     1561 attgtaattg cattctggta ttttgatgtg atggcatttt tatggctaaa tttagccgga
     1621 tgccttttgg tagttttaat tgctctggca atacaattct gcacacagaa tttgaacaga
     1681 atgttcaaca aaacaatatc taagtaaagt caattcgtat ctttggcaca ataattttct
     1741 cagataaaaa tcatgcaaga aataacagtt catgaattaa agcaatggct agatgaaggt
     1801 aaaaactttc agttgattga tattcgtgaa gaatatgaat acgatgcttg taatttaaac
     1861 ggaacgttga taccaatggg agaaatcata gacagaactg aagaaattgc aaaagacaag
     1921 cctgttgtta tacattgcag gtcggggtca aggagtgcag caatttatca agtacttagt
     1981 acacaatatg ggtttacaaa cctttttaat ttaaaaggtg gtataaaagc ctattcacaa
     2041 gaaattgatc cgacacttaa cgtaatataa attaaaactg tggaacactt aaaatttcta
     2101 attgatttta ttttacatat tgataaacat ttgttcgaac ttgtacaacg gtatgataca
     2161 tggatatatc ttatcttatt tctaattgta tttgctgaaa caggattggt tgtaactcca
     2221 cttttgccag gtgattcact tttgtttgca gcaggagctt tagctgcccc acctgataat
     2281 ccgttaaacg ttttttatct gatgataatt ttcttttttg ctgcttttgg tggagacaac
     2341 accaactatt ttatcggaaa ttatataggt ccaaaagttt ttgaaaagaa ctatcgtttt
     2401 ctcaaaaaag attatttaaa caaaacacaa gaattttatg aaaagcatgg tggtaaagca
     2461 gtaatatttg cacgttttgt tccaatagtg cgaacatttg ctccatttgt agccggtgtt
     2521 ggtagtatga cttataatcg ttttattggt ttcagtgttt tagcgagtat cttatggatt
     2581 aatatttgcg tttgggccgg ttattttttc ggcagattag agattgtgaa aaacaacttt
     2641 tcattagttg ttattgctat aatcggaatt tcattagtgc ccattttaat aggaataatc
     2701 aagcagaaat tctcttctaa atgaggaatt ttggtttaat tggttttcca ctaagtcatt
     2761 ccttttcaca aaaatttttt actgataaat ttaagaagct aaacttaaaa tgtgtttaca
     2821 ataattacga attaagtaac ctcaacaatt taagggaatt aatcagcaca aatcagctaa
     2881 ctggcttaaa tgttacttcg ccatataaaa caggtgtaat accctatctt gaccgattgg
     2941 aagaaacagc agaagctatt ggttcaatca atacaatatt gataaaggat aaaaaactaa
     3001 ttggatacaa tactgacata attggtttta aaataagtct tgagcgactt attgggaatc
     3061 aaaaacctcc aacattaata ttaggaacag gaggttctgc gaaaaccgtt gcttttgttc
     3121 taaagcaaaa aaatattcca tttcaattcg tttccagaaa gaaaaaagaa ggtgcaattt
     3181 cttatagtca atcagatgac tttttaagtc aaaatatttt gataattaat tgtacaccaa
     3241 tagggaggtt tcctgatttt ggcaaaaaac ctccaataaa ttacaccaag ttaaccgaca
     3301 agcactttct atttgactta aattacaatc ctgaaattac agcatttatg actgagggta
     3361 taaacaaagg gtgcaaggtt tgtaatggtt atgacatgct tattgagcaa gctgaggcct
     3421 cttgggttat ctggaatact tagttatgaa tacttaaaag cctgtttagc accattaatc
     3481 ctagttaaaa aagcattgaa taaacttttg aatctttcct gtcataagcc tatctttgcc
     3541 attaaaatat cagtaaatta tgaataacat tttaaaagac aacaaaacaa aatttataac
     3601 attatcaatt ctggtagttg ccatttcacg ccttttacca catccttata atttcactcc
     3661 tattggtgct attgcacttt ttgggggtac ttttcttaaa gataagaaga tggcttttgg
     3721 tcttccttta gcagcattgt tggtttccga tcttttatta caacttataa agccaggcag
     3781 tgcattttac gaaggatggt tttgggtgta cttaagtttt attctaataa caccattagg
     3841 ttttgtactc agaaacaaat taaaagtaca gaatgtagtt gcagcatctt taatgggatc
     3901 aataatattc tttgtactga ccaactttgg tgtatgggca acagggttaa tgtatccgct
     3961 taccggtgca ggtctaatga gttgctatgt tagcgcagtt ccattttttg gaggcactat
     4021 aatgggtgat ttatttttca atgcactact ttttggtgta tttgcatttg caaaatggag
     4081 aatgcctgta cttgtaaaag aagcttgata gaaaatttta taatcaattc aaataacatt
     4141 taatgtattt caacaatata cttgaaacca ttggtaacac gccattggta aaattgaatt
     4201 ctgtgacaca aggagttcag gctacagttt tggctaaaat cgaaactgta aatccaggca
     4261 actcgataaa agacagaatg gcactgaaga tgatagaaga tgccgaaaag agtggaaagt
     4321 taaaacccgg tggaacaatc atagaaggaa ctagtggtaa cacaggaatg ggattggcta
     4381 ttgctgcaat cattaaaggg tacaaatgta tttttacaac tactgataaa caaagcaaag
     4441 aaaaagtaga tgcattgcgt gcttttggtg cagaagtaat tgtttgccca actaatgttg
     4501 aaccagaaga ccctcgctcc tactattctg ttgcagccag actcagtaaa gaaattccaa
     4561 atgcatttta tccaaatcag tatgacaatt taagtaatcg tcaggcacat tatgagcaaa
     4621 ccggtccaga aatttgggaa cagacagaag ggaaaatcac acacttggta gttggtgctg
     4681 gtacaggcgg aacgataaca ggcactggtc gttatttaaa agaaaaaaat ccaaaaatta
     4741 aagtatgggc aattgacact tatggttcag cgcttaaaaa atatcatgaa acaggtattt
     4801 tagataagaa tgaaatttac ccttatgtaa cagagggtat aggagaagat tttataccac
     4861 agaattacga ttttactgta atagatcatt ttgaaaaagt tactgataag gatgcggcat
     4921 tgatgacacg tgatttggtt cgcaaagaag gtatttgggt tggtaacagt gccggctcgg
     4981 ctgttgcagg gttgcttcag atgaaagagc aattaaaacc aacagatgtc gttgtaatta
     5041 tttttcacga tcatggatcg aggtacattg caaaaatatt caatgatgac tggatgcgat
     5101 ctaaaggcta tttcgataag aaaggcatga cggcatcaga tattatcggc tcaaaaaaag
     5161 acgttaattt aactactgtt gaagcaactt taccgattgg cgatacattg aaaattataa
     5221 aaacaaataa tttttctcaa atacctatca cccgtgaagg aagaattgtt ggtaatatta
     5281 ccgagaatca tatttactcg tcacttttaa aaagtcctga actaaaaaat aagccggtta
     5341 gtgatattat gcaagaagct atgccattta ttgatatcaa tacttcaata gatacattaa
     5401 gtgacttgct tagtaaaaac aatcaggcag ttattgtaaa agattttaag gctgacagaa
     5461 actacattgt aacaaggcag gatgtagttg atgctttagc gtaaattcaa tatttagcag
     5521 aatatgtcat ttggcatttg tgaaatgagt cttgttccta tgaggaggga gccttcgcac
     5581 agaagtgagt tggtttcaca aatacttttt ggtgaacatt acgaagtagt tgaatcacaa
     5641 aaagagtggt ttaaaatcag aacttcgcat gatggctatg agggcttcat tgctttaaac
     5701 caacatacag aaataagtta taacgatttt caatttctac aaaaaaacgg agcatttgtt
     5761 tgctttgacc tggtgcaaat tttattacac gacaataaaa ttcagactgt gattttaggt
     5821 agtgctttac cattttttga aaatcagcat tgcagaatta ataccagaac ttaccggttt
     5881 gatggcaatg caaagtttcc tcaaaaagca gctacatcag cattattaat tgaaaatgca
     5941 tacatgtatc tcaatgcgcc atatttatgg ggaggaagat ctccttttgg tatagattgt
     6001 tccggattta cgcagttggt ttataaatta tcgggatatt ctcttttgcg cgatgctgct
     6061 ttacaagctg aacaaggaaa tattgtgaac cttgttgatg aagccatgcc gggcgattta
     6121 gcattttttg ataatgaaga agggaatatt attcacgttg gtattttaac tgcacgagat
     6181 accattattc atgcatctgg gaaagttcgt attgatgcat ttgatcaggt aggtatttac
     6241 aatcaggaaa ccaaacgata cacacataaa ctcaggttga taaaaagaat attttaaatt
     6301 catccatgaa taatccgcag ctcaaagctt ctgaaaattt tcctgcaaca gtaaatgaat
     6361 tatatgatga agagggtgaa ttggtattga ttttatatac tcatgaactg gttgatttgc
     6421 ccgaaggcct atggctatca acagaaggat attatgaagc agttattaaa aaaactgaaa
     6481 tccaaattga agatgccttg ttttgcatta gtgagctcaa ctccatatca ggaatgcaat
     6541 atgagcttac tttaagttag gagagtaata tccgattcaa attttgttgt aataaaatat
     6601 tcttctcaat tcttattgct gcgtattcca acttcttttc aattgcattt tgaatatcaa
     6661 tattactctg agtttgcatc atgaaaattt tattctcttc aagatatgaa gcaaggtatt
     6721 cctgttctgt ctgacccggt gtaggtacca ataatgctct tactcctaat tgtgcaatat
     6781 ccataattgt ggaatagcct gaccgacaaa tcaccaacgt atctgtattt atgaacttag
     6841 caatttcatc tgctggcata taatttttaa attgtaatgc tcccctatca ggaatttcat
     6901 ttgggtcgga aattttccca cgtattaata atgcactaca atggagtttt tctaactgtt
     6961 caattatttt accttcaaat attgttcgct gaggctccgg acctgataga attgcaacta
     7021 tatcatactt cttatcattt gacagatttg aagattgaaa tgaagaaagc aatccacaat
     7081 tatactttgg aattttaata aacttatttt cagataaaat tccggacagt ttaaatccat
     7141 gttccacatc aggaatccaa cattcatgaa aacgatttat atattttgca tgtaatctgt
     7201 taatggaagg ccgcaaaaac aacaaagatt ccggtgattg aatcattagt tgatgtgata
     7261 aaaaaatatt ctttgtaaaa tttgtaaaac aaccatacct gttatcagaa ataataattt
     7321 ctgcattata ttttgaagca attttcttta tagcggattg ttcattgcta atatttaaca
     7381 gcatttttgg tgtttgttta agcattgccc ataccatatt accattaaga gggtaaacag
     7441 gactatatga tggtaattct tctgcttgca gcacagggaa ttgccgcttc aatacactta
     7501 agctattgcc atcagatgca ataatcactt tgcaaccggc agctaaaaga ttatcaatta
     7561 cagttttact tcttgttgcg tgacctagtc cccaatttaa gggagctaca atgactgttt
     7621 tactcataaa atcaatccga aataaatcac ttaactaatc caatgcatgt ttattaaaca
     7681 atatatagcc aatatggaaa aggacagtaa atgattttgt ttttctgtca taataattaa
     7741 gtgtcattgc tacaggacct accggactgt ggaaaattcc ggaaaatgat gcaataaaat
     7801 atctggcaga taatgcatca ccaaatactg ttttctcatt atcattttta acaactgcct
     7861 gaaatggttg aaaagcataa ccctctaaac ggaaatcaat attcgaacgt aaactaaata
     7921 catttcgaac tccaatacct aaatagtttg gtgcatgaaa attgtcgaga aataaagttc
     7981 tactttcctg agtgggataa aatccaggca tttgtaaaat agtggctgta taattactga
     8041 agaatggttg acctgaaaaa tttgtttcag cataaaaccc gagtttatac cttcctgctc
     8101 tgaaaaaata attatcgtaa atcattttaa cctgccacca ttgatgccac tctctgatag
     8161 ttggggcatt atcttgtaaa gaaccgggat tggttacttc attgccggaa acaaaacgtg
     8221 ttcgaatagc taagtaagaa ccttttgatg catacatctt cctatttaaa gtgtttcttt
     8281 caaaaccaac aacacaactt aatccctcaa acctggtaaa gtctgcagta tcattaacag
     8341 agaaacttct ggtttgataa tacctgtctt taatgttaaa ataatttcca cctacagtta
     8401 gtgctccttt atttcgtgcc ggcataccaa tatttaatcc ataggactgg tctgttacta
     8461 ataaatatgc aggtttgata tcatctttaa aaaaactact gcttttaaaa tagtccatcc
     8521 tgtttaaagt agtaaacggc tcaataaaaa agtcaggtct gccgggtata tcgagcctca
     8581 atttaagctg accggaatta tatagttttc cgaaataggt attggcatgg atacttaaac
     8641 tatatggacc aagaatatta tatcggaagc caataaaccc ttcactaatt ggtcgtgaag
     8701 agaaatttcc tcctatatca actcgcaaat ctgtttccgg tttaatataa atctccagat
     8761 tatatacttc tgttttagga tcataaacca attttggaaa tgcatatctt atattttcat
     8821 ctgcaagcaa tttaaaataa cggggtttga gttgtcgaag tagcatattg cttccgcgag
     8881 gattcaaaat agacttaata tattttgttt tttcggggct tacacctgtt actacaatgt
     8941 cgccaactat taaaggatgt aatttgctaa taaagttatc tcgtttctgg gttaattctt
     9001 cgggtgagat tcttctggca atagcttgtt tgatatcatc cattcgagcc attgttgctt
     9061 tataaccact atcaatcaaa gcttgaaaat tataaaaatc aaatactcca atattggtaa
     9121 catcaggttg aattaaaata tcatcagctc cgggcaatac aacatgctgc gattgaatta
     9181 tcattgttct gagttgtgaa aacatattgt cttctttcgg aatatcaggg ccaccgccca
     9241 ctgaaacacc aatcataata tcaggatcaa actcattgag cataacatct gagggaaagt
     9301 tattataaat tccaccatcg tagtaaagct gattatcggc tgtcataggt gagagataca
     9361 gcggataggc aattgaggct cttattgcct gactaaggtc gccacaatta aaaattaccg
     9421 ggcgtttgtt tttaatatct gaagcaacac aacgaaatgg aacaaagagg ttattaaaat
     9481 cataatcggc accggcactt gcagctgaat gattttcaag cagtgcaaag tctatttgtc
     9541 cactattgac caatgctcca ttaagattca ttcttaaagt tgaatcatag ctaagtttaa
     9601 cagttgccca tgatgcatct ctttcgcgtt ccttgaaaaa atatataagt tcatcgctca
     9661 aggtgcctgt tgcccaattt ttaaaatcgt cacttaaaac aatattttgc atttgttgtg
     9721 ggctggtacc tgaaacatac atgcctccaa caagagctcc tgctgaagtt cctgtaataa
     9781 aatcaatagg aattccattt tcttccaatg cttttatcac gccaatatgg gcaaatcctc
     9841 ttgcaccgcc tccacttaat accaaaccaa ctttttgcgc aaatagaata tttacattta
     9901 ataaactaat tatagataac acctttatcc atttctgcat catcgtgcaa aggtattatt
     9961 ctgacacatt tgaattactg tcgcatagtt gatggaaata atcagcagct tttatcatac
    10021 ggctgccata ccatgcaaaa aattcaccat caaccaattt tatctcagct ttaggacaga
    10081 tcgttttaaa ttcttcaata tggcgtgctt taaaaggata tggctctgaa gaaagcagaa
    10141 taatttcagg attaagttct gccattttct tttctgaaag ctcaggatat cttttgtgat
    10201 agtacatcag attatcatag ccggccatgt tcatcatatc gcttataaat gtatcacctc
    10261 ccacacacat ataggggttt ctccaaataa aatacaatgc tctttttgct gtatgttttt
    10321 tatttttgat ggtaagagca gcaaattttt ccgcaatctg actattgatt tctgaagcgc
    10381 gactctgaca atcggtcatt acacctatat tataaatcat gtccaatgct tcgtgaagat
    10441 tactaacatc acttaaccag accggaaaat tttttcttag ttgctcaata gcttcgcgat
    10501 tgttttcttc tttattagct ataataatat cgggcttcag ttggttgatt aactcatgat
    10561 tcacagattt tgttccaccg acccgaggta cagataaaaa aagttcgggt ggataaatgc
    10621 aatattttgt aataccaact attctatctt gtagtcctaa atctgcaagc aactctgttt
    10681 gtgatgggac taaggaaaca attctttgag gtggtgtttc caatttaatt tcaaatcctg
    10741 tttgatctat aaacttcaac atgtcaggta aagttaagca taaaaaaagc cggtattacc
    10801 cggcttttac atatttttta aaccaacact attttattaa ttctcccatc atgttcattt
    10861 tttgttttgg cataagggtt attcctgatg atgtatgacc atcttcatca attgtgacac
    10921 cggtttccaa tttcatggct ttctgtacag gagtaaaaat gccattagtt acaatttgaa
    10981 gcttaccatc ttttaattca aaatgttctt caactgcctt cggaacaaga acgttattga
    11041 atgttaaaat ctctccctgt tgtaactgcg ctgtctttcc atcgggatat tttatcattc
    11101 cacgttgatt aatcgttgca ccattatcaa gtgtttgatc gctctccaga aaatttactg
    11161 tgttgccttt aatataatag acaaggccat tgcgaacaaa gaaacccgat tctgttgatt
    11221 ttttttcagt ctgttgactg aaagacacca ttgtgcttag taaaattgca aagcaaatag
    11281 ctgaactcat gaataacttt ctcattgatt tttattttat aaaagtaaaa ttacgatgaa
    11341 ataaattaaa aaacaacccc ttacttttta ggatcgtaaa ttacattgtt gccacgttgc
    11401 ttgccagagt acaacttgtg tctggtagca ggtttttcat tagctgcacc ggcagcagga
    11461 tcaccttcgg gagcaactgc agttgaatcg ggtacagcct cagtagctgc tgtggagtct
    11521 tctgtgcttt ctgtagtttc tgtaggtggt ggtgtagttt gttctgtttc ctgtgtagta
    11581 gttgactgcg aaaatgctac agttgagata atcaacattg caccaagggc agacaagcgt
    11641 ttaactaact ttttcattgt attatttttt tccaaaatta ataatcaaaa cgatgattca
    11701 acaatcatat tttattttta ttgcattcag atgttaataa atcaagactt cacaatcaaa
    11761 agcactattt cattggaatt tggcttttta cttaggataa ataaaaaagg gaaacaacta
    11821 ttatgttgtt cccctctaac ctaaaaacaa taaaccctcc acaaatgtag gagaataccg
    11881 ttttgaggat cagattgtta ataacttttt atttacccat tttcttaaca tctgcaatca
    11941 acttttcatt cattttaatg taactgttat cgctgctttc ctttgctaaa gccagacttt
    12001 gctctgctgc ttttgttgct tcggtatagt ttttcagttt atattcaatt ttagctttag
    12061 tgtgtaaaac ccaatatgcc tttgggttgt tttccacagc tttatttatc cattcgcgtg
    12121 ctttattcag gtctttatca ttttcgtaat aatagtttgc agcctgatag tagggcttgt
    12181 tatctcccat agccttatct atttgcttca taacattctc gtcaatattg gtagtaacag
    12241 gaatactcac ttttgtttta tcccataaca aatcaatact gcatgaagtt ggtgcaatgt
    12301 tattgatatt gatagtgaaa gtttccatct tatcggcagt agcaaccggc ttcaccttaa
    12361 atcgcatcat ttcatctgtt tgtttatagt cgcctacatt tccgccaaga gttaaatctt
    12421 tgtaaaaaat aatttcccaa tctgttgtgt ttggaattgt gtagagtgca taagtgccgg
    12481 ccgcaatatc attaccattt accttggtat ctgctccaaa agttactttg gtagattgat
    12541 tggctcctgt gcgccatatt ttaccataag gaacaaggtc gccaaaaatt gttcttcctt
    12601 ttactgatgg tctgctgtat tcgattttga tttcgccaag accaaatgcc tgtgtcagac
    12661 tttgagttgg acttggttgt ggcgttttta attgcgccat tgaagttgtt gttgtggcaa
    12721 tgatgagtgc cattaacgat gatgtgaatt tttgcatgat aatttaaatt tgattttgaa
    12781 ttttatacct aaacataaaa acccgacaga caacatttag tttttatcgg gtttaaaaag
    12841 tttgcatttg agattgaata aattaaatgt tcagttcttt aaaagtatta ccttcttgta
    12901 attttcctgt ctgatagcct tttttaaacc agtacattct ctgttcagaa gtgccatgag
    12961 taaaagcatc aggcacaaca tagccttgtg cttttttttg aatcttatca tcgccaactg
    13021 cacttgctgc acttaatgct tcttcaatat caccatcttc caacacattg tttaattttt
    13081 gattttgatt agcccaaact cctgcataaa aatctgcctg aagttctaaa gcaactgaca
    13141 atttatttgc ttctgtttcc gataaacctt cctgcatctg atgtacctta tccgatgtgc
    13201 ctaaaagatg ttgaacatga tgacctactt catgtgccaa tacataagca acagcaaaat
    13261 caccgccttc tgctccaaaa cggcttttca actcatcaaa aaatgaaagg tcaatatata
    13321 ctttctcatc ggccggacag tagaacggcc caacagcaga gcttgcattg ccacaaccgg
    13381 actgtgttgc atctctaaaa agaaccaatt taggctcacg atattctaat tcgttttcct
    13441 gaaaaatttt cttccagata tcttcgttat ctgctaatgc tacttttata aaacttgcca
    13501 tttcatcatc ggcagcagat gattccgtgg tggttgtctg agttgttgcc tgctgctgca
    13561 tttgttgtag aagttgatta ggatcaccac ccatcagcaa gttgatgaca atgacaatta
    13621 tgccaactaa tccaccacct aaggcaattt tacctcctga aaatctcctg cggtcttcaa
    13681 cattgtcgct ttcgcgcctg cccatccatt tcataatatc tgttttacag agcaaataaa
    13741 gcaattattt tggaaatact atttaatgac ggcataaatc tgaaagtgat tatatttgat
    13801 gcttcataat aacctcaaca tgcaaaatgc acttaaattt attttagcat atctcttttt
    13861 cacaggaaat gtttctgctc aggaaattct tccaaactat atgactcaaa aagaaatgtt
    13921 gcagatgcca tcatatctcc aacaattgtc taatcaatta aaaaataatc agattgcagc
    13981 tccaccatca tcacccgttc gaactattgc tgagtgggaa gagctgcaag gaattttaat
    14041 tggctggaca agttaccctt ctattttgcg tgaaattgta cgtgctgcta aaacagaatg
    14101 ccgggtttat gttgtaacca ataatgagca aagtgtaatt aactatttaa acagtggaaa
    14161 tgtagatacg ataaatgtaa gttttgtgaa tacccctttc aattcagtat ggtcacgcga
    14221 ttacggtcca tggagtgctt acacaaacga tgttgacact ttagttacta ttgactggat
    14281 atataatcgt cctcgtcctg acgatgataa tgttccggtg gcaattgccg gattactaaa
    14341 tacgccatta taccaaacca caatgtcgcc atacagtttg gttcatacag gtggtaattt
    14401 tatgtgcgat ggatttggaa cgggattctc aagcaatctt atagtgaatg aaaatccctc
    14461 gttaaccata agtcagatag atactatcat gaaacaattt atgggcatta acagatatat
    14521 taaaatgcct acattacctt atgatgctat ccatcatatt gatatgcaca tgaagcttct
    14581 taatgaagaa acaatcttga tgggataata tcctcaaggt gttgcannnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn tccgtatttg gcacacctta taaaataatc cgcattccaa
    14701 tgcctgctga caatggtgct tatccaaata caaacggtga ttattttact tacaccaatt
    14761 catcgttcat caataatact attattgttc ctacttataa tattcctcaa gacagcacag
    14821 cattacggat atatagagat gcattaccgg gatataatgt tattggtatt aactcacttc
    14881 aaagtattgg tgctatagga gcattgcatt gtatcactaa agatttaggc actaatgatc
    14941 cgcttctgat ttcacatcaa caacttagca acacttacga tacaattaac ccctatcatg
    15001 ttgaagcatt tattaaacac cgtagtggaa ttcaatcggc aaccttatat tacaggacag
    15061 atactttgca accttataat actgtttcga tgaatgctat atcagggatg agtgattact
    15121 ttgaaacgta cattcctgca caatctggtg gaacaagaat ttactactat attgaagcat
    15181 attcaaatac ctcaaaacat cagacacgac caatgcctgc accgcaaggt tatttttatt
    15241 ttgatgtatt acagactact ggaataactg aagttttaaa tcagttatcg gtgggacaaa
    15301 ttttcccaaa tcctgcatcg gcagtgacat gcctccccta ctatagtcgc attggcacat
    15361 tgcttcagat taaaatcagt gatgtaacag gcaagatggt ttatgaatca caaaatacaa
    15421 caaagtctaa tggacaaaac tttgcgtttt ttgatgtcag tcaatttaaa ccgggagtgt
    15481 attttgtaaa atcagaaact aattcatcaa taaatattca gaaacttata gtaaagtaat
    15541 tttatcagag ataattgctg aataacacaa ttgactatta ccttttaact acaattattc
    15601 tggtggcaat attatttttt gatttatcaa aaaattgcaa gttatataca ccatccttga
    15661 gattagtcaa atctaaactt ccatctttca aattatacat ttccttgaca attcttccat
    15721 taatgtctga tattttcaaa gatgatatct cggaattatg cagaac
//
LOCUS       DAORQT010000019        15653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_347_length_15653_cov_13.542121, whole genome shotgun sequence.
ACCESSION   DAORQT010000019 DAORQT010000000
VERSION     DAORQT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_347_length_15653_cov_13.542121"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1028)
                     /locus_tag="PK134_01555"
     CDS             complement(216..1028)
                     /locus_tag="PK134_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB37570.1"
                     /translation="MYNLLKGKRGIIFGALDENSIAWKTAQRAHQEGAMFTLTNAPVA
                     MRMGEINKLAAECNAQVIPADATSIEDLENLFTKSMEILGGKLDFVLHSIGMSPNVRK
                     GKHYTALDYEYFHKTLDVSALSLHKTLQTAMKLDALNEWSSVVALTYIAAQRVFPHYN
                     DMGDAKSLLESITRSFGYHYGMAKKVRVNTISQSPTATTAGSGIKGFNAFYNYADVMS
                     PLGNASADECANYTVMMFSDFTRKVTMQNLFHDGGFSFTGVSLNAMEKFMEP"
     assembly_gap    1101..1146
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1147..2986)
                     /locus_tag="PK134_01560"
     CDS             complement(<1147..2986)
                     /locus_tag="PK134_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB37571.1"
                     /translation="MPFKKSIYITTSAILIIVALLQWSFITVKENEYQQQALEAYLSK
                     SSLDFSGELEKLKILAGSNKVNSQQLIAQFKACRLAYKKLEGFTGYYFAYTERDLNGP
                     PIPMVEEEPQSKYVRPPHGLQVIEEIISEKPLNKAQLLDEINHTLGLAAKLATSFKTL
                     HPYDYQLFEIMQLRLVKIYTLGINNFDCQIIKTGIPETKAALFSISNILNISYRNADF
                     SMVNNSIDSAVAFLDRQQIRVNQGAEFDYFTFYRNYVVPVNLSLINARKNILKFDPQN
                     IRALNLDATTLFEKEAYNTYFFTKLQRSNNGDMLPYLGKLLFFDPILSVNNAVSCASC
                     HKPNMAYTDGLPKSYGFLKNDTLPRNTPTILNAALQRNLFMDASVLVLEDQAKTVVQN
                     EREMHGDFKVVVAKLNQSSTYKKIFSQAFAGTKDTAISEEGILKALAEFQRTQLSFDS
                     PFDKAMRGAGTIEQPVIDGYNIFMGKAKCGHCHFAGLFNGTTPPQYRESEFDIIGVPS
                     TNKRPYKLDGDLGRYNVFKNDELKYAFKTTTVRNIAVTAPYMHNGVYKTLQEVVDFYD
                     KGGGAGLGIHLPDQTLPDDKLKLTKDEKKKLILFLQSLTDTSAVVHF"
     gene            3208..5025
                     /locus_tag="PK134_01565"
     CDS             3208..5025
                     /locus_tag="PK134_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRB37572.1"
                     /translation="MNYLSVENISKSFGDRVLFNNISFGIAKGQKLALVARNGTGKTS
                     LLRILAGLDVPDEGTVTFRNQLKVSYLTQENTLPQLATVADCLTENTHISDALLNEKP
                     HYELTEEEQTHLGKIKSVAGKLGVPFFHQKVNELSGGQQKRLALSKVLIDEADFIIMD
                     EPTNHLDLDMIEWLESFLASTNITMLLVTHDRYFLDEICDEILELEDGKLYRHKGNYS
                     YYLEKKAERDEMESANLQRSKNLFRTELDWMRKQPRARGTKSKSRIDAFHVLEEKTKK
                     KNEQGNVALDINISRIGSKILEIHHISKSYGDLKLIDNFDYSLRKGEKIGIVGSNGAG
                     KSTLLNIIMGKEPCDKGKIVMGETIVPGYFEQQSKNISDQKRVIEVIREIADFIPMSG
                     GRKISASQLLERFLFDPSMHFQYVEKLSGGEKRRLYLLTVLMKNPNLLILDEPTNDLD
                     IQTLSVLEDYLASFEGCVIIVTHDRFFMDRVADHIWAFEGDGKISVLNGNYYDYRQML
                     KEQKREQKKEAAAVVRTKPEETTANKKRKLSFKEKQEYEKLENDIMLLEEEKTMLEQQ
                     MGEAELTHEKLMEVSNRFQQVLHDIDAKTMRWMELAEIG"
     gene            complement(5033..6067)
                     /locus_tag="PK134_01570"
     CDS             complement(5033..6067)
                     /locus_tag="PK134_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HRB37573.1"
                     /translation="MGIYIKSSVCISARNTFGETDLSEHSLQTEKLMAREPVYSMIAP
                     AVLRRMGKAVRMTCGAVSKLNLENHPVNGIIIGTANGGMEDCIRFLNQIVQYDEGTLT
                     PTNFVQSTPNAIASTIGMNHQCRGYNITHVHRGNAFENALLDAFMYLVENKNSTLLLG
                     GIDEISDYNYNIERLGGWYKNEIPVNGLYESKTEGSIAGEGCAAFTVSNNSESALLEI
                     VDVSTLHSTDVDEVLQQLKQFCDKHALNLSETMLVSGHNGDVRNETLHYAVESHFQQS
                     AIIRYKHFFGEFPTVSALALWLIGQAATNSGILPEHFYLRKSESKIKNYLICNHYKGY
                     QHGFILTKTP"
     gene            6250..6984
                     /gene="gldF"
                     /locus_tag="PK134_01575"
     CDS             6250..6984
                     /gene="gldF"
                     /locus_tag="PK134_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRB37574.1"
                     /translation="MYSLFKKEINSFLNSLIGYISIAVFLIVIGLFLWVFPGNDLNIA
                     DSGYAAVDSLFNITPWVYMFLIPAITMRMFSEEKRTGTLEILLTKPLTEFQIVLAKYL
                     SGLVLVIFSLLPTLVYVVTVYKYAAPVGNIDLGGIAGSYIGLFFLASGFVAIGVFASS
                     LADNQLVAFIIAMFLCLLCYTGFQSVATILATGVVANLIDQLGIVAHYNSMSRGVIDS
                     RDVIYFISLTALFLVFTRLKLESRKW"
     gene            6986..8671
                     /gene="gldG"
                     /locus_tag="PK134_01580"
     CDS             6986..8671
                     /gene="gldG"
                     /locus_tag="PK134_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRB37575.1"
                     /translation="MKKKIRSANLYQFLLLLLVIAGLNAAGKFLFWRFDLTTEKRFTL
                     SPTTKEKLKQLKDIVYVKVYLTGDLPAGFQRLSSATRDMLMEMKSYAGSNLEFDFIDP
                     SALADSKQRNELFNQLADKGLQPTNISERNAEGTSERILFPGAILNYLSKEQPLLLLK
                     DRMGASAEEMVNTSIQNLEYEIINAISKISTRKPATIGILHGQGELDKPYLADFYKSV
                     SASYPTHYVQIKNQLNALQDYDCLVIAKPDSAFDEKDKFIIDQFIMRGGKVLWLLDMM
                     SADMDSLARKNEFIAIPKELNLDDMLFRYGVRLNKDLVMDLQSVPIPILTGYVGNRPQ
                     NSLLPWFYYPLVTPTSKHPIVNNLNAIRFAFAGSIDTIVSAHIKKTILLQSSHYCKTV
                     QSPAVVDLNILRKDPDEKEYRNPPVNLAVLLEGSFRSNFANRIPYQIATDSTIGFKAD
                     GKPTSMIVISDGDVIRNDYKRSSNTVYPLGMDRYTGQFYGNKNLLLNCIDYLCDNSGL
                     MALRAKEFKLRLLDKTRFDPDKKMLQIVNVALPVLLIILFAMIKLLMRKKKFS"
     gene            8679..9704
                     /locus_tag="PK134_01585"
     CDS             8679..9704
                     /locus_tag="PK134_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRB37576.1"
                     /translation="MNKNRILLLIVVLLLSVTGYYYFNNSDSTIKKELRDFAVADTSS
                     IDKMFLADKKGNKALLERKGPALWTINGKYPARQDAINFLLKTIKQIEVRSPVGKAAL
                     QNVLKELSSTGTKIEIYSKGDKLKTYYVGGPTQDMLGTFMYLENSTVPFVIHIPGFNG
                     YLTPRYFASADEWRSRIIFQYGEGQIASVMVQNNQHPDESFRIKKDDDHYVYYSSLTA
                     TAPAEIFQSQLVAYLAKYQLIGFERPTYDLDVAYRDSLLKTGPVRILEVEGVDGKKDK
                     IEMYLKPVDTGTMTSVDERTGLLREFDPDRMYASWNNDTNLIVVQYHVFDKLFSKPDR
                     IKGFGSL"
     gene            9771..10892
                     /gene="dnaN"
                     /locus_tag="PK134_01590"
     CDS             9771..10892
                     /gene="dnaN"
                     /locus_tag="PK134_01590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRB37577.1"
                     /translation="MKLIVSSLVLQKHLQSISGVLTTNSPLPILDNFLFAVNGSELTL
                     SASDLETTITTRLKVESKDKGIIAIPGKILLDILKTFNEQPLTFTINEKNHTIEISSD
                     YGKYKLNGQNGEDFPRIPVLEKSNHIELPSSVLHEAINKTIFATGNDDLRPVMSGVFC
                     QLSQDSTIFVATDAHRLVRYRRTDIKSKEAGSFILPKKPLNLLKNILPQEETRVKVDY
                     NEANAFFSFDNYDLICRLIDGRYPNYEAVIPVDNPNVLTVDRVAFLNSIKRVSIFSNK
                     TTYQVKLAIKGSELAISAEDVDYANEANERLTCSYDGSDMDIAFNARFLADIVSNLDT
                     EEIHLHMSLSNRAGLLTPGKDHTNAAEDILMLIMPVMLG"
     gene            10957..11682
                     /locus_tag="PK134_01595"
     CDS             10957..11682
                     /locus_tag="PK134_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRB37578.1"
                     /translation="METRQQKYAERYKDVIGASFSFETAQPELPLYYFVSGFAHPFLP
                     VIKHDGLFLFEWGLIPSWIKDAKGASEIRTKTLNAVGETVFEKPSFRKSIVSNRCLLG
                     VSGFFEWRDVNKKKYPYFIKTNNSELFSLGCIYETWVDKNTGELHNTFSIITTVANPL
                     MEKIHNVKKRMPLIIAEEDERKWVDPSTGIDEIRSLIKPYNESEMTAYTISQAANSAR
                     ANRNVPEILNAVNYPELALLDTI"
     gene            complement(11685..12434)
                     /locus_tag="PK134_01600"
     CDS             complement(11685..12434)
                     /locus_tag="PK134_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRB37579.1"
                     /translation="MKNIKAIVVDDEMFGREQIKLLITKYFPQITLVAEVANAAEALK
                     QIYFHKPDLIFLDVEMPDSNGFDLLEKIQEHDFEIIFVTAFDQYAIKAIKFSALDYLL
                     KPLNKEEFKSAVQKFLDRQKSEYDRKSVINNFIRNIKSEEDKLFRLAVNTSNKTYFLL
                     PDEIIRCEADGNYTKITCGNKEVVLSSKTLKEFDDLLNSYNFIRVHRAHLVNKKYIRS
                     FENENKLVLSDNSAIEVSRRKIAEVRNLFGQ"
     gene            complement(12467..15355)
                     /locus_tag="PK134_01605"
     CDS             complement(12467..15355)
                     /locus_tag="PK134_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB37580.1"
                     /translation="MMRFVIFILLTFYPLISVYAQDASRIPVFLNNLQTATTDSARVE
                     AYRDLCFNYSIANTDSGIYYGKKGLEIAQRIKFLKGIGDSYNSLGWCYSRKGDYTKAK
                     KHLTLALQYFEQTNDKCNVAVVLSNIGSTYFNQSLYADALEYYLKSVDLSNACTDAIR
                     KPAGLYSIGIVYNSQGEYNKAIPYFREAAALNEQINDSNKLADCINGIGNAYLGLEMY
                     DSAFHYLNQSSAMYQKLSNISGTAYAAESIGSIYLKQGNFDMALKQFEFARENFEKSG
                     SQNDLCYELTLIGDAYRKYGKINSAIIYMKMALQIADSNRFQTLQLQTLNSLSELYAM
                     KHDYQNAYLTFKQAAEIKDTINETKQQARLDEIKTKFETEQKDKMIEILNRDKAIEKS
                     NAEWQRQLKNIFIVGAVMLFLILLNLYNRFSLKKRAAQQLEQKNIIIAQEKERAERSE
                     KFKQQFLANMSHEIRTPLNAIFGMTELLQETKQDEQSSKYLQVIKQSSESLLVIINDI
                     LDLSKIEAGKMEIEKTPFRFRETINHVYQTFSHKAASKELDLKFSVGENVPELIIADP
                     FRITQVLINLVGNAIKFTERGFVIVSINQVRASQKHGLNYCDLLFEVSDSGIGIPAGQ
                     QEKIFESFQQTYAGNSRKYGGTGLGLTISQSLVQLMGGNIEVSSVENQGTIFSFTIEV
                     EVADALAETNKSEQVPIQLIIDKDAFSVLLIEDNEFNQILVKDALSKKMRDIKLQIVG
                     NGRDAIEILKNQKFNLILMDISMPEMDGYEITQIIRNELKESVGEIPIVALTANVSQH
                     EAEKCIAAGMNDYLSKPFKISELTDVINRFKGDVDEQKIDSRIKVAPIASSVDLAYLR
                     DFTDGDIQEMLRYVDMYLQRIPEVFNEIEISLKGDEEKHVIVLLHSIKPLIASVGLND
                     CVQKISELEKQRETIKKNKLMPVVLLIRRQCMTMVDSLKLWKVNNSAG"
BASE COUNT         4849 a         2837 c         2917 g         5004 t
ORIGIN      
        1 gcttattgaa aattaaaaag ttgcgcaaaa gtaaatttta tttttgaata atgtgactga
       61 cggtataaag cagggtgctt attgatttaa taattaggct taaaattttg attgtttaca
      121 gcgtaggatt taataatttt tggtgatggt gaatcgtaaa aaaaatcatc tcacaattta
      181 gaatgcaatg gtgagatgat ttatatataa tgagcctatg gctccataaa tttttccatt
      241 gcattgaggc tgacaccggt gaaggagaag ccaccatcgt gaaagagatt ttgcatggtt
      301 accttgcgtg tgaaatcgga aaacatcatt acagtgtagt ttgcacattc atcggcagag
      361 gcattgccaa gaggcgacat tacatcggca tagttataga aggcgttgaa accttttatg
      421 ccactgcctg cagttgttgc agtgggcgat tgagaaatgg tgtttactct tacttttttt
      481 gccatgccat aatgatagcc aaagctgcgt gtgatgcttt ctaaaagcga ttttgcatcg
      541 cccatatcat tgtaatgagg gaagacacgt tgtgctgcaa tgtaggtgag tgccactaca
      601 ctactccact cattgagggc atcgagtttc atggctgtct gcaatgtttt gtgcaggctt
      661 agtgcagaaa catccaatgt tttatgaaaa tattcgtagt ctaatgctgt gtaatgtttg
      721 cctttacgga cattgggcga catgccaatg ctgtgtaaaa cgaaatctaa tttaccgcct
      781 aaaatttcca ttgatttggt aaacaggttt tcgaggtctt caatagaagt ggcatctgcc
      841 ggaatgactt gcgcattaca ttctgctgca agtttgttta tttcgcccat gcgcatagca
      901 acaggagcgt tggttaatgt aaacattgct ccttcctgat gtgcacgttg tgcggttttc
      961 catgcaatag agttttcgtc taatgcgcca aaaatgattc cacgttttcc ttttaacaaa
     1021 ttgtacataa gttatgttgt tttgcggcta aagtaataga aattgatgaa actgcattaa
     1081 tctgtgccac cggcaatgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnntaaa gtgcacaacg gcagaagtat cggtgagcga ttgcagaaac agaatcagct
     1201 ttttcttttc gtctttagtg agttttaatt tatcatcggg aagggtttgg tccggaaggt
     1261 gaatgcccag ccctgcccca cctcctttgt cataaaaatc aacaacctcc tgaagcgttt
     1321 tgtaaactcc gttgtgcatg tagggtgcag tcactgcaat gttacggaca gtagttgttt
     1381 tgaatgcata tttaagctcg tcatttttaa aaacattgta gcgcccaagg tcgccatcta
     1441 atttataagg acgtttgttg gttgagggca cacctataat atcaaactca ctttctctgt
     1501 actgtggcgg tgtggttccg ttaaagagtc ctgcaaaatg gcagtgcccg catttggctt
     1561 tgcccataaa aatattgtag ccatcaatga caggttgttc tattgtgcct gcaccacgca
     1621 tggctttatc aaaaggcgaa tcgaacgaca gttgtgtgcg ctgaaattct gccaatgctt
     1681 ttagaatgcc ttcttcgctt atggcagtat cttttgttcc tgcaaaagcc tgactgaata
     1741 tttttttgta ggttgaagac tgattgagtt tggcaacgac aactttaaaa tcgccatgca
     1801 tctctctctc attctgaaca acagtttttg cctgatcttc gagaaccaat acactggcat
     1861 ccataaacag attgcgctgc aatgcagcat ttaaaattgt tggtgtgttg cgaggcagtg
     1921 tgtcgttttt taaaaaacca taagacttag gaaggccatc ggtataagcc atattgggtt
     1981 tatggcacga tgcacatgac acagcattgt tgacagaaag tatagggtcg aaaaataaaa
     2041 gtttgccaag ataaggaagc atatctccgt tgttgctgcg ctgaagtttg gtaaagaaat
     2101 aggtgttgta ggcttctttc tcaaaaagtg ttgtagcatc aagatttaag gctcttatat
     2161 tttgcgggtc gaattttaaa atattttttc ttgcattgat gagtgataaa tttacaggca
     2221 caacatagtt gcgatagaat gtaaagtaat cgaactccgc accttggttt actcttattt
     2281 gctgacggtc gagaaaagct acggcagagt caatgctgtt gttgaccatt gaaaaatcgg
     2341 cattgcgata agagatattt aaaatattgc tgatagaaaa cagtgctgct ttagtttctg
     2401 gtattcctgt cttaataatc tgacagtcga aattgtttat acccaatgtg taaattttta
     2461 caaggcgcag ttgcattatt tcgaacaact gataatcgta ggggtgcagt gttttaaacg
     2521 atgtggctaa cttggcagcc agaccaagtg tgtgattgat ttcatcgagc agttgtgctt
     2581 tattcaatgg tttttcggaa attatttctt ctattacctg caagccatgt ggtggacgaa
     2641 catatttgct ctgcggttct tcttcgacca ttggaatggg cgggccgttg aggtcgcgtt
     2701 ctgtataagc aaaataatat ccggtgaagc cttcgagttt tttataggca aggcgacagg
     2761 ctttaaattg tgcaatcaac tgctgtgagt ttactttatt gctgccggca agaattttaa
     2821 gtttttcgag ttctccagag aaatctaatg acgattttga gagataggct tctaatgcct
     2881 gctgctgata ttcattttct ttgaccgtta tgaacgacca ttgcagtaat gctacaatga
     2941 tgagaattgc tgatgttgtt atgtatatgc ttttcttaaa tggcatcttt caaaaatgac
     3001 aaaaaactat atatgtgctt tgttgagaga aataaaatta ttaacgcaat agtggttata
     3061 ttttatgatt gtaaaaatga tttttgtcaa ttaaaacttt ctatgaaatt aaattcaagc
     3121 aagagaaaaa ttgccgtata taaaaacaat gctgattgcc attaaatagc atgataagta
     3181 gtactttcgc gcttcaatta aaaagcagtg aattatttat cggtagaaaa tataagcaag
     3241 agttttggcg acagagtatt attcaacaac atttcgttcg gcattgccaa aggacagaaa
     3301 ctggcattgg tggcaagaaa tggcaccggt aaaacatcac tactgagaat tttagcagga
     3361 cttgatgttc ctgatgaagg tactgttact ttcagaaatc agttgaaggt gagttatctt
     3421 actcaggaaa atactttacc acagttggca acggttgcag attgcctgac agagaacaca
     3481 cacatttctg atgccttgct gaatgaaaaa ccacattatg aactgactga agaagagcaa
     3541 acacatttgg gaaaaataaa atctgttgca ggaaaattgg gtgttccatt ttttcatcaa
     3601 aaggtgaatg aactttcggg cgggcagcaa aaacgtttgg cgctatcgaa agtgctgatt
     3661 gatgaggctg actttatcat tatggatgag cctaccaacc acttagactt agacatgatt
     3721 gaatggcttg aatcgttttt agcatcaaca aacataacga tgttgcttgt tacacacgac
     3781 cgttattttt tagatgaaat atgtgatgaa atacttgaac ttgaagacgg gaaactgtac
     3841 agacataagg gcaactacag ctactattta gagaagaaag cagagcgtga tgaaatggaa
     3901 tcggcaaacc tgcagcgttc aaaaaatcta tttcgtacag aattggattg gatgcgaaaa
     3961 caaccacggg cacgtggcac aaaatcaaaa tcgcggatag atgcctttca tgtgcttgaa
     4021 gaaaaaacta aaaagaaaaa tgaacaaggt aatgtggcac ttgacatcaa catttcgaga
     4081 ataggaagta aaattctgga aatacaccac atcagcaaat cgtatggcga cctgaaactg
     4141 attgacaact ttgactactc gctgcgtaaa ggcgagaaaa ttggaattgt tggcagcaat
     4201 ggtgcaggaa aaagtacact gctgaatatc ataatgggaa aagagccttg cgataaaggt
     4261 aagattgtga tgggtgaaac tattgtgccc ggatattttg agcagcaatc gaaaaatatt
     4321 tcagatcaga agcgggttat tgaagtgatt cgtgaaattg ctgactttat tccgatgagt
     4381 ggtggacgaa aaatatctgc atcgcagttg cttgaacgtt ttctatttga cccgtccatg
     4441 cattttcagt atgtagaaaa attgagtggt ggcgaaaaac gcagattgta tttgctcacg
     4501 gtgctgatga aaaacccgaa ccttttgata ttggatgagc caaccaatga tttagatata
     4561 cagacacttt ctgttcttga agattatttg gcatcgtttg aaggctgtgt gattattgta
     4621 acgcacgatc gtttttttat ggacagggtt gcagaccaca tctgggcatt tgaaggagat
     4681 ggaaaaatca gtgttctgaa tggcaattac tatgactaca gacaaatgct gaaggagcaa
     4741 aaaagagagc agaaaaagga agcagcagca gtagtgagaa caaaaccgga agaaacaaca
     4801 gcgaataaaa aacgcaagct ttcattcaaa gaaaagcagg agtacgaaaa gttggagaat
     4861 gacattatgc tgcttgaaga ggaaaaaaca atgttggaac aacagatggg cgaagcagaa
     4921 cttacacatg aaaagttaat ggaagtaagt aatcgctttc agcaggtgct gcatgacatt
     4981 gatgcaaaga caatgcgttg gatggaactt gcagaaatag gctgatattt acttatggtg
     5041 ttttagtcaa tataaaacca tgctgatagc ctttgtaatg attgcagata aggtagtttt
     5101 taattttact ttctgactta cgtaagtaaa aatgttcagg caaaataccg gagtttgttg
     5161 cagcctgacc aataagccaa agtgccaatg ccgacaccgt tggaaattca ccaaaaaaat
     5221 gtttatatct gatgattgca gattgctgaa aatgactttc aacagcataa tgcaatgttt
     5281 cattcctgac atcgccattg tgacctgaaa ccaacatagt ttccgaaaga tttaaagcat
     5341 gtttatcgca aaattgtttt agctgttgta aaacttcgtc aacatcagta ctgtgaagtg
     5401 tgcttacgtc aacaatttct aacagtgcac tttctgaatt gttacttact gtaaacgctg
     5461 cacaaccctc accggcaatg cttccttctg tttttgattc atacaaacca tttacaggaa
     5521 tttcgttctt ataccatcca cccaagcgtt ctatattgta attatagtca gaaatttcat
     5581 caatgccacc gagcaataag gttgagtttt tattttctac taaatacatg aaagcatcaa
     5641 gcaaagcatt ttcaaaagca ttgccacggt gaacatgcgt tatgttgtag ccacgacatt
     5701 gatgattcat tccaatggtt gaagcaatgg cattgggtgt gctctgcaca aaattggttg
     5761 gtgttaatgt gccttcatca tactgcacaa tctgattaag aaaacgaata caatcttcca
     5821 taccgccatt tgcagtacct attattattc cgtttacagg gtgattttcc agattgagtt
     5881 tgctcacagc accgcaggtc atgcgcacag ctttacccat tcttcgcaaa acggcaggtg
     5941 caatcatact gtaaacaggt tcgcgcgcca taagcttttc tgtttgcaag ctatgttctg
     6001 aaagatctgt ttcgccaaaa gtatttctgg cgctgatgca tactgaagat ttaatgtaaa
     6061 tacccatgat tttgaaaaag caagattaag aaatttggct tcatcgaaaa gtacttctgc
     6121 aagcaaatta tttaattaac aattttgttt ttataagctt ccttaatctt tcataatctg
     6181 caactgccta tatgcaacga aactgtattt ttggcagtct ttatttcagc attgctgctt
     6241 taaattatta tgtattcact tttcaagaaa gagataaaca gttttcttaa ttcactaata
     6301 ggatatattt ccattgctgt ttttttaatt gtgattggat tatttctgtg ggtgtttccg
     6361 ggcaacgacc tcaacattgc agacagtggt tatgctgccg tagatagttt attcaacatt
     6421 acaccatggg tttacatgtt tttgattcct gccataacca tgcgcatgtt ttcggaagaa
     6481 aaacgtacag ggactttgga gatattgctt accaaacctt taacagagtt tcagattgta
     6541 ttggcaaaat atttatcagg attggtattg gtaatatttt cattgctgcc aacattggtt
     6601 tatgtggtta cagtttataa atatgctgca cccgtaggaa atattgattt gggtggaatt
     6661 gcaggaagtt atataggatt gttttttctg gcatcgggat ttgttgccat tggtgttttt
     6721 gcttcatcat tagcagataa tcaattggta gcatttatca tagccatgtt tttatgcctg
     6781 ctatgctaca caggatttca gtctgttgca acaattctgg caacaggtgt tgttgcaaac
     6841 ctgatagatc aacttggtat tgtggcacat tacaactcaa tgagcagagg tgttattgac
     6901 tcgcgcgatg taatttattt tatcagtttg acagcgttat ttttagtgtt tacccgatta
     6961 aaactcgaaa gcaggaagtg gtgatatgaa gaagaagata cgcagtgcca acctctatca
     7021 gtttttgctt ttgcttttag tgattgcagg cctcaatgct gccggtaaat ttttattctg
     7081 gcgttttgac cttactactg aaaaacgttt taccctttcg cccacaacaa aagaaaaact
     7141 aaagcaactt aaagatatag tatatgtaaa agtttatctg acaggtgatt tacctgcagg
     7201 ttttcagagg ctaagttctg caacacgcga catgctgatg gaaatgaaga gctatgcagg
     7261 ctcaaatctt gagtttgatt ttattgaccc atcggcatta gcagacagca agcaacgtaa
     7321 tgagcttttt aatcaacttg cagacaaagg gcttcaacca accaatatct ctgagcgaaa
     7381 tgcagaaggc acatcagaaa gaattttgtt tccgggagca atacttaact acctttctaa
     7441 agagcaacca ctactactgc tgaaagatag aatgggtgca tctgcagaag agatggtgaa
     7501 cacttctatt cagaatctgg aatatgaaat tattaatgcc atttctaaaa tttcgacaag
     7561 aaagccagcg acaattggta ttttacatgg tcagggagaa ttggacaaac cttatctggc
     7621 tgatttttat aaatctgtat ctgcctccta ccctacgcat tatgtgcaaa taaaaaatca
     7681 gctcaatgct ttacaagatt atgattgttt ggtaatcgca aagcctgact ctgcatttga
     7741 tgaaaaagat aagtttataa tagatcagtt tatcatgcgt ggcggcaagg ttctttggtt
     7801 gctcgatatg atgagtgctg atatggatag tttagcaaga aaaaacgaat tcattgctat
     7861 tcctaaagaa ctgaatcttg atgacatgtt gtttcgttat ggtgtaagac tgaataaaga
     7921 cttggtaatg gatttacagt cggtaccgat tcctatactg acaggatatg taggtaacag
     7981 accacagaat tctttgttgc cctggtttta ttatcctttg gtgactccaa catcaaaaca
     8041 tcctattgta aataatctaa atgccatacg ctttgcattt gccggaagca ttgacaccat
     8101 tgtttctgcg cacattaaaa aaactatatt gttacaaagt tcgcactact gcaagacagt
     8161 tcaatcaccg gcagtggtag atttaaatat cctgcgcaaa gatcctgatg aaaaagaata
     8221 tcgtaaccca cctgtaaatc ttgccgtttt gcttgaaggt tcattccgct ctaattttgc
     8281 aaacagaatt ccatatcaga ttgcaacaga cagcaccata ggatttaaag cagatggtaa
     8341 accaacgagc atgattgtga tttctgatgg tgatgttatc cgtaatgatt ataaacgtag
     8401 cagcaatacg gtttatccgc ttgggatgga tcgttatacc ggacagtttt acggaaataa
     8461 aaatctattg ctaaattgta ttgattattt atgcgataat tccggattga tggctcttag
     8521 agcaaaagaa tttaaactgc gcctgttaga taaaacacga tttgaccctg ataaaaaaat
     8581 gctgcaaata gtaaatgttg cattgcctgt tcttttaata attcttttcg caatgattaa
     8641 gttgttgatg cggaaaaaga aattctcctg atagaataat gaataaaaac cgaattttac
     8701 ttctcatagt tgtgttgttg ctttctgtaa ccggatatta ttatttcaac aacagcgata
     8761 gtacaattaa aaaagaactg cgcgattttg cagttgccga tacttcttca attgataaaa
     8821 tgtttctggc tgataaaaaa ggaaacaaag ctttgctgga aagaaaaggc cctgccctct
     8881 ggactattaa cggtaaatat cctgcacgtc aggatgcaat taattttttg ctcaaaacaa
     8941 taaaacaaat tgaagtacga agtcctgttg gtaaagctgc cctgcaaaac gtactgaaag
     9001 agttgagcag cacaggaaca aaaattgaga tttacagcaa aggcgataaa ctgaaaactt
     9061 attacgttgg cggaccaaca caggatatgc ttggcacctt tatgtatctt gaaaattcta
     9121 ccgtaccctt tgtgattcat attccgggct ttaacggata ccttacacct cgttattttg
     9181 ccagtgccga tgaatggcgc agccgcatta tttttcaata tggcgaaggg caaattgctt
     9241 ctgttatggt tcagaataac caacatcccg atgagtcttt cagaataaaa aaagatgatg
     9301 atcattatgt ttattattca agcctgactg caactgcacc ggcagaaatt tttcaatctc
     9361 agttagttgc atacttagca aaataccagc ttataggatt tgagcggcca acctacgacc
     9421 ttgatgttgc ctatcgcgat tcgttattaa agacagggcc tgtcagaata cttgaagttg
     9481 aaggtgttga tggcaaaaaa gataaaattg aaatgtatct gaagcctgtt gacacgggaa
     9541 ccatgaccag tgttgatgaa agaacaggcc ttttgcgcga atttgaccct gacagaatgt
     9601 atgcctcttg gaataacgac accaatttga ttgtggttca gtatcatgta tttgacaaac
     9661 tatttagtaa acccgacaga atcaaggggt tcggtagtct ttaatttttc tttaatttag
     9721 ccttcacaaa gttccatctg attatatttg ccacctaact tttgatattt atgaaactaa
     9781 ttgtttccag ccttgtactt caaaaacatt tacaatctat cagcggggtt ttaaccacta
     9841 actctcccct gccgatttta gacaattttc tgtttgccgt taatggcagt gagcttactt
     9901 tatcagcttc tgaccttgaa acaacaataa ccacaagact taaagttgaa tctaaagaca
     9961 aaggcattat tgccatcccg ggtaaaattc tgctcgacat cttaaaaact tttaatgagc
    10021 agccgcttac ttttaccatc aatgaaaaaa accataccat tgagataagc tcagattatg
    10081 gaaagtataa actcaatggt cagaatggtg aagactttcc gcgcataccg gttctcgaaa
    10141 aaagtaacca cattgaattg ccatcatctg ttttgcatga ggcgataaat aaaaccattt
    10201 ttgctacggg caatgatgac ttgcgccctg taatgtcagg tgtattttgt cagctttcgc
    10261 aggacagcac catttttgtt gctactgatg cacatcgttt ggtgcgctat cgcagaacag
    10321 atataaagtc aaaagaagca ggctcattta ttctacctaa aaagccgttg aaccttctga
    10381 aaaatatttt acctcaggaa gaaacacgcg taaaggttga ctataacgaa gccaatgcat
    10441 ttttcagttt tgacaactat gatttgattt gccgtttgat tgatggacgt tatccgaact
    10501 acgaagcagt aattcctgtt gacaatccaa atgttttaac tgttgacaga gttgcatttc
    10561 tgaactcgat aaaacgtgtt tctatttttt caaacaagac aacttatcag gtaaaacttg
    10621 caataaaggg aagcgaattg gcaatttctg ctgaagatgt agattatgcc aacgaagcca
    10681 atgagcgttt aacctgctct tatgatggat ctgatatgga cattgcattt aatgcgcgtt
    10741 ttcttgcaga tattgtttca aatcttgaca ccgaagagat tcatctgcac atgtcattgt
    10801 cgaaccgtgc cggattatta actcccggaa aagaccatac caatgcagca gaagatattc
    10861 tgatgctcat tatgcctgtt atgcttggct gataaatttt tttgccggtt tatattgtta
    10921 agaatgtaaa tttttatgtg ttacaatatt gcaataatgg aaacccggca gcaaaaatat
    10981 gccgaacgtt ataaggatgt tattggagca tcattcagct ttgaaacagc acaacctgaa
    11041 ttaccacttt actattttgt cagtggattt gcacatccgt ttttaccagt aatcaaacat
    11101 gatggactat ttttatttga atggggactc ataccctcat ggataaaaga tgctaaaggc
    11161 gcttcggaaa taagaactaa aacactcaat gccgttggtg aaacagtttt cgaaaaacca
    11221 tcattcagga aaagtattgt tagcaatcgt tgtttgcttg gtgtttcagg cttttttgaa
    11281 tggcgcgatg taaacaaaaa gaaatatcct tattttatta aaacaaacaa ctctgaatta
    11341 ttttctcttg gctgcattta tgaaacctgg gttgataaaa acaccggtga attacataat
    11401 actttcagca tcatcactac tgtggcaaat ccactgatgg aaaaaataca taacgtaaaa
    11461 aaaaggatgc cgttgataat tgcagaagaa gatgaaagga agtgggtgga tccaagcaca
    11521 ggtattgacg aaattagatc actgattaaa ccatataatg aatctgaaat gacggcctat
    11581 accatttcgc aggcagcaaa cagtgcaagg gctaatcgta atgttccgga gattttaaat
    11641 gcagtaaatt acccggaatt ggcactcttg gatacaatct gaaattattg accaaataaa
    11701 tttctgactt ctgcaatttt tcttcttgaa acttcgatgg cagaattatc actcagcacc
    11761 aatttgtttt cgttttcaaa tgaccttata tattttttgt tgactaaatg cgctctgtgt
    11821 actctgatga aattataact gttaagtaaa tcgtcaaact cttttaatgt ttttgatgat
    11881 agaacaacct ctttattgcc acaagtaatc ttagtataat ttccatcagc ttcgcatctg
    11941 ataatttcat cgggaaggag aaaatatgtt ttatttgaag tgttaacggc caatcggaac
    12001 agcttatctt cttctgactt aatattcctg ataaaattat ttatgacaga ttttctgtcg
    12061 tactcgcttt tctgtctgtc gagaaatttc tgcactgcac ttttaaattc ttctttattc
    12121 agaggtttta gcaaataatc taatgccgaa aatttaattg ctttaattgc atattggtca
    12181 aaagctgtaa caaaaattat ttcaaagtca tgttcctgta ttttctcaag caaatcaaaa
    12241 ccattactgt ctggcatttc aacatctaaa aaaatcaaat caggtttgtg aaaataaatc
    12301 tgctttagtg cttctgctgc attagctact tcagcaacta atgtaatttg agggaagtat
    12361 tttgttatca ataattttat ctgctctcta ccgaacattt catcatcaac cacaattgcc
    12421 tttatatttt tcacctcact aatgtaatta ttgtacaaat atatttttag ccggcagaat
    12481 tatttacttt ccatagcttt aaagaatcaa ccattgtcat acattgcctt cttataagta
    12541 atacaacagg cattaacttg tttttcttta tagtttctct ctgcttttcg agttctgaaa
    12601 ttttttgcac acagtcattc aaaccaacag aagcaatgag aggtttaatg ctgtgtaata
    12661 agacaattac atgtttctct tcatctcctt ttaaagaaat ttcaatctca ttaaaaacct
    12721 cagggattct ttggagatac atatcaacat aacgcaacat ctcctgaata tcgccatcag
    12781 taaagtctct cagatatgct aaatcaactg aagatgcaat tggcgcaacc ttaattctgc
    12841 tgtcaatttt ctgttcatca acatcacctt taaaccggtt tattacatcc gtcagttcag
    12901 aaattttaaa aggttttgaa agatagtcgt tcatacctgc ggcaatacat ttctctgctt
    12961 catgttgcga tacatttgca gtgagtgcaa ctatcggaat ttctccaact gattctttaa
    13021 gctcattccg aataatctga gtaatttcat acccatccat ttcaggcatt gaaatgtcca
    13081 ttaaaatcag gttgaacttt tggtttttca gtatttctat tgcatccctt ccatttccaa
    13141 caatttgcaa ttttatatca cgcattttct ttgaaagtgc atctttaact agtatctgat
    13201 taaactcatt atcttcaatc agcaaaacag aaaacgcatc tttatcaatt atcaactgaa
    13261 tgggaacttg ctcagattta ttcgtctctg ctaaagcatc tgcaacttct acttctattg
    13321 taaaggagaa tattgtgccc tgattttcta cactactaac ctctatattc ccacccatta
    13381 actgaacaag actttgcgaa atcgtcaaac caagacctgt accaccatat tttctagaat
    13441 ttcctgcata tgtttgctga aaagattcaa atattttttc ttgctgacca gcaggtattc
    13501 caataccact atccgatact tcaaaaagaa gatcacaata atttagtcca tgcttttgtg
    13561 atgctcttac ttgattaatt gaaacaataa caaatccacg ttcagtaaat ttaattgcat
    13621 ttccaactaa atttataagt acttgtgtga tacgaaaggg atctgctatg attaattcag
    13681 gcacattttc tcctacagaa aatttcaaat cgagttcttt actcgcagct ttatgagaaa
    13741 aagtctgata aacatgatta atagtttctc tgaatctgaa tggagttttt tcaatctcca
    13801 ttttaccggc ttctattttt gataaatcaa gaatatcatt aataatgacc aacaaacttt
    13861 ctgacgattg tttaatgact tgtaaatact tactactttg ctcatcttgc ttagtttcct
    13921 gcaacaattc agtcattccg aaaatggcat ttaatggcgt tctgatttca tgactcatat
    13981 tagccagaaa ttgttgctta aatttttcac tgcgttcagc acgctctttc tcctgtgcaa
    14041 tgatgatgtt tttctgctct agctgctgtg ctgccctttt ctttaatgaa aaacggttat
    14101 acagatttaa caagatgaga aatagcatca ctgctcctac aataaaaata ttctttagtt
    14161 gtcgttgcca ttcggcattc gatttctcaa tagccttatc tctattgagt atttcaatca
    14221 ttttatcttt ctgctcagtt tcgaattttg tttttatctc atcaagtcgg gcttgctgct
    14281 ttgtttcatt gatagtgtct ttaatttccg ctgcttgctt aaaagtcaga tatgcattct
    14341 gataatcatg tttcattgca tacaactcac ttaacgaatt tagtgtctgt aattgtaaag
    14401 tttgaaatct attgctatct gctatctgca aagccatttt catataaatg attgcagaat
    14461 ttattttacc atatttcctg taagcatctc ctatcaaggt aagttcataa cataaatcat
    14521 tctggctgcc tgacttttca aaattctctc tggcgaactc aaattgcttc aatgccatat
    14581 caaaatttcc ttgtttgaga taaatacttc caatactctc tgctgcatag gccgttccac
    14641 ttatgttaga taacttttga tacatagcag aagattgatt caggtaatga aatgcactat
    14701 catacatttc taaaccaaga tatgcattac caattccatt tatgcaatca gccagtttat
    14761 tactatcatt tatctgctca tttagtgctg cagcttctct aaaataagga atggccttat
    14821 tatattctcc ttggctgtta tacacaatgc ctatagaata taatcctgcc ggctttcgaa
    14881 tagcatcagt acatgcattg gataaatcaa cactcttcag ataatactct aaggcatcag
    14941 catataaact ctgattaaag tatgtgctgc cgatattgga taatactact gccacattac
    15001 atttatcatt ggtctgttca aaatattgaa gagctaatgt aagatgcttc tttgctttag
    15061 tataatctcc cttacgagaa taacaccaac ctaagctatt ataactatca ccaatgccct
    15121 ttaaaaactt gattctttgt gcaatctcaa gtcccttttt accatagtat attccactgt
    15181 ccgtatttgc aatactgtaa ttaaaacata aatcacgata ggcttccact cttgcagaat
    15241 cagtagtagc cgtttgaaga ttatttaaaa aaacaggaat tctagatgca tcctgcgcat
    15301 aaacagaaat taacggataa aaagtcagta aaataaaaat aacaaaacgc atcatctata
    15361 attagttgcc aaatgagtta ccaaagtaag aaaactttta atagaatact taccgtttaa
    15421 taattaaaca caaccattca ataagttaat gcaatatgta tatcatatag caactaatat
    15481 tggattataa attctttcaa aaaatatgtt taactattaa aaacaaaaaa tatgaaactc
    15541 ctttacacct tagcactttc actttttacc gcacatttaa catttgctca attcagtagc
    15601 gatccctcac aaccaatgcc ggtctgcaat gctgcacatg gacagtggaa tat
//
LOCUS       DAORQT010000020        15191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_374_length_15191_cov_10.661734, whole genome shotgun sequence.
ACCESSION   DAORQT010000020 DAORQT010000000
VERSION     DAORQT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_15191_cov_10.661734"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..375)
                     /locus_tag="PK134_01610"
     CDS             complement(178..375)
                     /locus_tag="PK134_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37581.1"
                     /translation="MPKEKPEDIQPVVTYQTEIIEIVRPVEMDMDDNDAKMDEKEMMD
                     DDVSFNDDLFSGSSDDDDDDM"
     gene            complement(600..797)
                     /locus_tag="PK134_01615"
     CDS             complement(600..797)
                     /locus_tag="PK134_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37582.1"
                     /translation="MAKASKSKKSAAKKKAKPARKASGKKAAKKKPVAKKKATKKKAA
                     PKKKAATKKKAAPKKVLEKEV"
     gene            994..2610
                     /locus_tag="PK134_01620"
     CDS             994..2610
                     /locus_tag="PK134_01620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRB37583.1"
                     /translation="MLLLLLICIGCSQKPIADLILFNGKIYTVDSTFSTAQAMVVSNG
                     KIVETGTNALADKYQCQQKIDLMGKSVYPGFIDAHCHFYGYSTDLSKCPLFGTKSFDE
                     VIQKVKEYAATNKFSWILGRGWDQNDWSENEFPDKSVLDSLFPNTPVYLMRIDGHAVL
                     VNQKALDIAGITTSTKVQGGEIITKNNHLTGLLIDNAVDIVKAHIPEFTAQIKTEGLI
                     KGEQNCFAVGLTSVTDAGLRVSDIRLLDSLQKAGTLSMKIYAMAELTNESKKYWFEKG
                     KYKTERLEVRSFKLYADGALGSRGACLLQPYSDLPGHYGFILQDKDSIQLAAKEVVEH
                     GFQLNTHCIGDSANRFMLKVYAANLKTKNDLRWRIEHCQVVNHDDLKYFSDYSIIPSV
                     QPTHATSDMYWAEKRLGSERIHDAYSNNALLKETGMLALGSDFPVEDINPLYGFYAAV
                     VRKDQKGFPPEGFETENKISREEALRGMTIWAAYSMFEENEKGSLEKGKAADFVIMND
                     DLMTAPDSILFKLPIETTYSNGIKVYENKK"
     gene            2579..4051
                     /locus_tag="PK134_01625"
     CDS             2579..4051
                     /locus_tag="PK134_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37584.1"
                     /translation="MALKFMKTKSKIIFFLLLFCSVSAFGQNLIWNPTVKLNDRPYPK
                     FLGVNNGYFYAAGSNIKMIQGASDSIFLATAWKKLKLTLLRYDDKFILKDTAAIRFLQ
                     FPVRVYDVVLSDSTIKIFYSKGVGNLEFCADVFDLKGYFKQTVVLIAGKDVAKYNQKN
                     FFSYNTSINNRFFTITTNDSIYCFSNQLVSAWKTSLPNSTFFDGAVNDSGTFSGIFQR
                     DKEYFIATSVEGKIITQTISAEKEELSDFKIIHEGNRIIVVSLFGFTDNTFNPIYNDI
                     ANANKFKSNGISISEFDNNLQLIKTNSIKFSEETLLETVDNLLLKNIKGVDFLKIQQM
                     DVMSNGNIMLLIEKQFASYTKPADVNGKKVASIGRETSANELLLICTDMQDKNMQTVI
                     KRKVSATENFEYVCNVKGIPHKDDYYLYFLDGRNPEEYQMTQSIYNSQLRRIYTKEMD
                     LASAKKLLPDMSSITRLDKNRFILYARLLKKFSTAVLDFK"
     gene            complement(4179..4517)
                     /locus_tag="PK134_01630"
     CDS             complement(4179..4517)
                     /locus_tag="PK134_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37585.1"
                     /translation="MKKALSVLSVLGLFLIVNAYANSNKANTVTDLQVTESSIVANHD
                     MPCDGKAEKDKDCCKKKSKKKKCCADKAEASAASCADKQAKAGCCAHKKAGAGASDVK
                     TDTKSDESAK"
     gene            complement(4630..5364)
                     /locus_tag="PK134_01635"
     CDS             complement(4630..5364)
                     /locus_tag="PK134_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRB37586.1"
                     /translation="MSEAGKPIVEVCLTPALFYQYNTKESIVVAIDILRATSSICVAL
                     QYGARSIVPVMTVEESFQYKEKGYLIGAERQGSMVEGFDLGNSPFSYMEDKIIGRDIA
                     LTTTNGTKAINIANGSAGIVAGSFLNLDVLAAWLIEQNKDVLCLCAGWKNTFNLEDTL
                     FAGALSQKLLPHFQLSEHRDSAIAAMNLYNLAKHDMYDFLKDSSHRTRLERLKIDEDV
                     LYCLTPNQTLVVPVLKDGVLKKHSDW"
     gene            complement(5368..6468)
                     /gene="gcvT"
                     /locus_tag="PK134_01640"
     CDS             complement(5368..6468)
                     /gene="gcvT"
                     /locus_tag="PK134_01640"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRB37587.1"
                     /translation="MEENTTLKHTALTAIHQKAGAKMVPFAGYLMPVQYTGINDEHET
                     VRNAVGMFDVSHMGEFILKGDDALDLLQRVTSNDASKLSAGKAQYSYFPNETGGIIDD
                     LIVYCIDEKTFMLVVNASNIEKDWNWLQKHNNKKVEMHDISDKTSLLAVQGPKAMETL
                     QKLTSIALADIPYYNFAKGEFAGCQNVLISNTGYTGAGGFELYFENQYAEKIWNDVMQ
                     AGQPFDIKPAGLGSRDTLRLEMGFCLYGNDIDDNTSPLEAGLGWITKFTKNFTNSDAL
                     KQQKEQGIANKLVGFEMIDRGIPRHDYEICNASGQKIGRVTSGTQSPTLNKAIGMGYV
                     SKDQSAEGTEIFIKIREKLIKAAVVKIPFIKK"
     gene            6586..8079
                     /locus_tag="PK134_01645"
     CDS             6586..8079
                     /locus_tag="PK134_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRB37588.1"
                     /translation="MKLLTSEQFRMADAYTIKTEPISSVDLMERAAICCSDEIAKFTD
                     EKDHICILCGPGNNGGDGLAIARILADAGKNVSVLLTDNSNKLSPDAEANFLRLQKTS
                     VKIFYLTDANLPFKNTTFWVDAIFGTGLNKAVEKNIADLIHQVNSTRTPVISIDLPSG
                     LFADKHTPTNVAVIHASTTLTFQRLKRSMLYAENAHRIGEVKVLDIGLDEHYMESLPT
                     TQELVDRSLINKILKPRKRFSHKGNFGHALLIGGSYGKAGAIILSAKACVKSGAGLTT
                     VHTVKTTGATLQSAIPEAMLSTCEGDKVCDLPDLAPYNAIAAGPGLGINEQTSSMLKL
                     LLQKAKKTLVLDADALNIISQNKNLIDNLPENTILTPHKKEFERLFGTFENDFQRNDF
                     QIAASVKHKIIIVLKGAYTSISTPEGKCFFNTTGNAGLAKGGSGDTLTGIITALLAQG
                     LNATDAAIAAVYIHGLAADCAVKKIHPYSLTASDVTEFLSEAFIQTV"
     gene            complement(8063..10069)
                     /gene="ispG"
                     /locus_tag="PK134_01650"
     CDS             complement(8063..10069)
                     /gene="ispG"
                     /locus_tag="PK134_01650"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRB37589.1"
                     /translation="MDSALTDQNIFQLYCNSLTSYQRLKTLEVKIGDVAMGANHPIRV
                     QSMTTTDTLDTKATVEQSIRMIVAGCEFVRITAPSINEARNLQNIKDELRKRGYKTPL
                     IADIHFTPNAAETAARIVEKVRVNPGNYADKKKFDTIEYTDVTYAEELERIRERFIPL
                     VKICKEYGTAMRIGTNHGSLSDRIMSRYGDTPLGMVESALEFLRICMDEGYYSIVLSM
                     KASNPQVMVQAYRLLIAKMRAEFMDLNNMQNTLPALFPLHLGVTEAGEAEDGRIKSAS
                     GIGTLLEDGIGDTIRVSLTEDPELEIPVAQKLVSRYNNRTSHQTIKPINHHKLPYSPF
                     SYSKRLTKAVLNVGGNHVPVVIADFSGKQNISPASLFPVGYNYSQSLDKWHISDQACD
                     FIYVGNQHISFALPGTLGVIYNNKMWLQHSDDERTYPLFNVNELNDALKCTAAIKFIE
                     LNTEDFFKQQLQVDKLSDAVLVLKTSNDHAMPDLRNVFVDMMQQKNTLPVIIKRDYAK
                     ADLETFRLHCSTDAGALLLDGMGDGVWLCSELVSPADINQTAFGILQAARTRISKTEY
                     ISCPSCGRTLFDLQETTAKIRARTHHLKGIKIGIMGCIVNGPGEMADADYGYVGSGAG
                     KITLYKGKEVVRRNIDAALAVDALIELIRDNGDWIEPAVITPSV"
     gene            complement(10142..11050)
                     /locus_tag="PK134_01655"
     CDS             complement(10142..11050)
                     /locus_tag="PK134_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37590.1"
                     /translation="MKKIIFVLLIACSHFVAKAQFAPVGAQWHYTQCTVTFPYSCGPV
                     EVNATGTTVINGITCTNLQIAPFTCVGFNYLPVYQSNDTVYRFLYNLNTFTMLYDFNA
                     QPGDIWNIVSNTINQPPPAPLTDTVRIRVDSVNMVNINNHPRKIFYISYADSSHIPFV
                     FEGPIIEGIGSLNFLLTQFNLCDPQITAFRCYNDSTLGLYQPNTLVDCDSVVVVGESE
                     VIKKDYVSLYPNPFTDQICIHSEDFLYRKYDIKLVNIVGKVVYHSKQRQALDQLTIYP
                     KNLAPGTYYLLLESDKKVLQKRVVKL"
     gene            complement(11092..12180)
                     /locus_tag="PK134_01660"
     CDS             complement(11092..12180)
                     /locus_tag="PK134_01660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB37591.1"
                     /translation="MSHNKKKILLICPYPEDTAPGQRLRYEQYLNLFRKEGYDIHVSS
                     FMTYRFNSIVYKPGNVPEKIFWTLFGYIKRFLQLFTLWRYDAVYIFLWVTPFGLPLFE
                     WLFVLLNPNMIYDIDDMVFLGHSSKANKFIKILKGTRKITFLMKKAKHIVVSTPSNEE
                     FARKYNNAITDISVAINTDVYLPANKYSNNDVITIGWSGSHSTSRYLFLLEEVLRKFA
                     GRSNIRILVIGDTTFKFNGFDCETIPWQSATEVKDLQKIDIGLYPLPDEQWVYGKSGG
                     KALQYMALGIPTIATGIGTNFRIIDDGESGFLVRTDEQWIEKIEYLINHPEERKRIGM
                     NGRMKVEKEYSLHVNKKYYLEIFEKVVG"
     gene            complement(12246..13463)
                     /locus_tag="PK134_01665"
     CDS             complement(12246..13463)
                     /locus_tag="PK134_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB37592.1"
                     /translation="MNVLYISYDGMTDNLGQSQVIPYLKGLTGYGHHFDIISCEKPDK
                     FKAKEIEIRKLLDSLNIGWYPLPYTKRPPVLSTVIDVVRMYRKAYSLHKENKYDAVHC
                     RSYLSAMIGQDMKKKFGTKFIFDMRGFWPDERIDGDLWNIKNPVYRIIYNYFKKKELQ
                     FFNESDLNVSLTHAGLNEIKKMNVSNEQINKFSIIPCCSDYELFKPLPRDLALVKQLG
                     FEASNKILCYLGSIGTWYMFDEMMAMFKQLHFQDNSFRLFIMTPEPPSMVFDSAKKLG
                     INTDLIKVVSATRQQVPKYLSIAHLGISFIKPCFSKLSSSPTKMGEMLAAGVPLICNS
                     GVGDVERIINETKTGVIIHGFTEDEMQSVAKRAIEMIQVPRENIREQSRPVFALESGV
                     KLYAQAYEKMQKQ"
     gene            complement(13528..>15191)
                     /locus_tag="PK134_01670"
     CDS             complement(13528..>15191)
                     /locus_tag="PK134_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRB37593.1"
                     /translation="GSTPNNFISQSNENSSNGYFGFIRSGADIFLDNRNTITVSGSYM
                     NGYFKPLEFLQTKTDTLFPGYTSSSLSDRNSSSKREFKNTGGTVAYKRLFPQEGKELT
                     ADINFNSVKSDMGGNYSTQNYLGTGEAYGNPILQKQSIEGENTFVTAQTDMDFPLDKK
                     SKIETGLRGAMRDYSSVNQNFIFDDSQQQYILIPNQTANYQYVDQVYAAYATYSKSFE
                     KLSLQAGLRAESSFYEGELTQTGQKFSTNYPVSLFPSASASYKLSSKTDLQINYSRRI
                     NRPNFFQIIPYSDYSDSLNISKGNPGLKPEFTHSVELTAMQTMSRKNTLMATVYYKQT
                     DGVITRYLSYETDTILGRNVLVSTFRNANTSYAYGLELTAQNNIKGWLDITSNINAYQ
                     SVINGKNIESNLKVEQFSWFAKLNLNVKLPKNFSVSISGDYQSKTALQLNSNSGGRGP
                     MGGGTQSTAQGYIRPNYGVDAGLRYDFLKDKAASLTLNVSDIFKTRVNDVHSESDFYI
                     QDSWRKRDAQIFRLNFSYRFGKFDTSLFKRKNQKINTEGMQDMSM"
BASE COUNT         4667 a         2912 c         2687 g         4925 t
ORIGIN      
        1 ataattccga attgttagtt gagaaattta aggtaaaaag taaaagtgac ggggaatgag
       61 aggactgttg ggtagagttt tcttaaagat taataaatgg ataaaaatca tcatacttaa
      121 gtcaacaaaa aaagccctgc aatgcagagc tttttttaaa tattaattat ttacatttta
      181 catatcatca tcgtcatcgt ctgatgagcc gctgaataaa tcatcgttaa atgacacatc
      241 atcgtccatc atttctttct cgtccatctt tgcatcatta tcatccatat ccatttctac
      301 cggcctaact atttcaataa tttctgtttg gtatgttacc acaggctgaa tatcctcagg
      361 tttttctttt ggcattggcg caggtttttt cactgccggt tttgatacca ctttttttat
      421 tacaacctta ggtgccgctt tctttttggt agcagctttt ttctttggag ctgctttctt
      481 tttagtagta gcctttttct ttggtgcttc tttctttttt gtttcttact ttttatattg
      541 tgctgaattt tctgtggtta ttctttttta attggcgcag cttttttctt tggcggtgtt
      601 tatacttctt tttctaaaac tttctttggc gcagccttct ttttggtagc tgcctttttc
      661 tttggtgctg ctttcttttt agtagccttc ttttttgcta caggtttctt tttagctgcc
      721 tttttaccac ttgctttgcg ggcaggtttt gcttttttct tagcagcaga tttttttgat
      781 tttgatgctt ttgccatagg taaacaagat tgatggtgag tatgtgttct attttttgcg
      841 taaatataca gcaggtttta attgatgcaa gaacaaatca aaaaatattt ttcaatacct
      901 gcctgtttct aagttgcgcc tttcttttaa agattttatg gatatttgat acctgatttt
      961 aaaattctaa cattgattaa gtcctttaaa ccaatgctgc tgctgctttt aatttgcata
     1021 ggatgcagcc aaaagccaat agcagacctg attcttttta acggtaaaat ttataccgtt
     1081 gacagcacct tttctacagc acaagcaatg gtggtttcta atggcaagat agtagaaacc
     1141 ggcaccaatg cattggcaga taagtatcag tgccagcaga aaatagacct tatgggtaaa
     1201 agtgtttatc cgggctttat tgatgctcac tgtcattttt atggctacag cacagacctt
     1261 tccaaatgcc cgctttttgg cacaaaatca tttgatgaag taatacaaaa ggtaaaagaa
     1321 tatgccgcta ccaataagtt cagttggatt ttaggacgtg gatgggatca aaatgactgg
     1381 tctgagaatg aatttcccga caagtctgtt ttggatagtt tgttcccaaa tacaccggtt
     1441 tatctgatgc gaattgatgg tcatgccgtt ttagtcaacc aaaaagcttt ggatattgca
     1501 ggtataacca cttctacaaa ggttcagggt ggtgagataa taactaaaaa caaccactta
     1561 accggactgc tgattgataa tgctgttgac attgtaaaag cacatattcc tgaatttaca
     1621 gctcagatta aaacggaagg acttataaaa ggcgaacaaa attgttttgc cgttggtctg
     1681 acctctgtta cagatgccgg attaagagtt tctgacattc gtttactcga ctcacttcaa
     1741 aaagccggaa cccttagcat gaaaatttat gccatggcag aactgacgaa tgagagtaaa
     1801 aaatactggt tcgaaaaagg aaaatataaa actgaaagac ttgaggtaag atcgtttaaa
     1861 ctttatgccg atggagcact tgggtcaaga ggtgcctgcc tgttgcaacc atactccgac
     1921 cttcccggac attatgggtt tatacttcaa gacaaggact ccattcaatt ggcagccaaa
     1981 gaagtagtgg agcatggatt tcagctaaac acgcattgta ttggtgattc tgcaaaccga
     2041 tttatgttaa aagtttatgc cgccaaccta aaaaccaaaa atgatttaag atggcgcatc
     2101 gaacattgtc aggttgtaaa tcatgatgac ttaaagtatt tttctgacta ttccataata
     2161 ccaagtgttc agccaacaca tgccacaagc gatatgtatt gggcagaaaa aagacttggc
     2221 agcgaaagaa tacacgatgc ctattcaaat aacgccttat taaaagaaac aggtatgtta
     2281 gccttaggca gcgactttcc ggttgaagac attaacccgc tttatggatt ttatgcagct
     2341 gtggttcgta aagatcagaa aggttttcca ccggaaggtt ttgaaacaga aaataaaatc
     2401 agtcgcgaag aagcattgcg cggcatgacc atttgggcag catattctat gtttgaagag
     2461 aatgaaaagg gctctttaga aaaaggcaaa gcagcagact ttgtaattat gaatgacgac
     2521 ctgatgactg caccggacag tatattattt aaattaccta tagaaacaac ttattcaaat
     2581 ggcattaaag tttatgaaaa caaaaagtaa aatcattttc ttcctcttat tattctgtag
     2641 tgtttctgcc tttgggcaaa acctgatatg gaacccgaca gtaaaattaa atgaccgtcc
     2701 atacccaaaa tttttaggcg taaacaatgg ctatttttat gctgccggca gcaatattaa
     2761 aatgattcag ggtgccagcg acagcatttt tttagcaaca gcctggaaaa agctcaaatt
     2821 aactttacta cgctacgatg ataaatttat tctaaaagat actgcggcta taagattttt
     2881 gcagtttccg gtgcgggtat atgatgttgt actgagcgat tcaaccataa aaatatttta
     2941 ttcaaaaggg gttgggaatc tggaattttg cgctgatgtt tttgatttaa aaggatattt
     3001 taagcaaaca gtagttttaa ttgcaggaaa agatgttgca aaatataatc agaagaattt
     3061 tttcagctat aatacttcca tcaataaccg tttctttacc attaccacta atgacagtat
     3121 ttattgtttc agcaatcagt tagtcagtgc atggaaaact tctttaccca acagtacatt
     3181 ttttgatgga gcagtaaatg atagcggcac tttttcaggt atattccaaa gagataaaga
     3241 atatttcatt gcaactagtg ttgaaggaaa aattatcact caaacaattt ctgctgaaaa
     3301 ggaagaatta tccgatttca aaattattca tgaaggcaac agaataattg tggtttcatt
     3361 atttggtttc actgacaata ctttcaatcc tatttataac gatattgcca atgcaaacaa
     3421 atttaaaagt aatggtataa gcatttcgga gttcgacaac aaccttcaac ttattaaaac
     3481 aaatagcata aaattctctg aagaaacatt actcgaaacg gttgacaact tattactaaa
     3541 gaacattaaa ggcgttgact ttttaaaaat tcagcaaatg gatgtgatga gtaatggcaa
     3601 tattatgcta cttattgaaa agcaatttgc ctcctacacc aaacctgctg atgtgaatgg
     3661 taaaaaagtt gcatcaatcg gtagggagac ttctgcaaac gaactgcttt taatttgtac
     3721 cgatatgcaa gataaaaata tgcagactgt tattaaaaga aaagtaagtg caacagaaaa
     3781 tttcgagtac gtttgtaatg ttaaaggtat tccacataaa gacgactact acctttattt
     3841 tttagatgga agaaatccgg aagagtatca gatgacgcaa tctatttaca attctcagct
     3901 caggagaatt tacactaagg aaatggattt ggcttcggca aaaaaacttc tgcctgatat
     3961 gtcatctatt acacgcctag ataagaacag gtttatcctg tatgcaagac tgttaaagaa
     4021 attttctact gcagttttag acttcaaata aaaatgttta gtaataggca ataaaaaacc
     4081 gctctcaata atgagaacgg ttttcaattt tcggccctaa ccgtgggata ttacgcagca
     4141 ttgcaagcta aatactgcac caccttggtg ggttaaaatt attttgcaga ttcgtctgac
     4201 ttagtgtcgg tcttaacatc agatgcaccg gcaccagctt ttttatgtgc gcagcaacct
     4261 gcttttgctt gtttgtcggc acatgatgca gctgaagctt ctgctttgtc agcacaacat
     4321 tttttctttt tgcttttctt tttgcagcaa tctttgtctt tttctgcctt accatcacat
     4381 ggcatgtcat gatttgctac aatactgctt tcagttacct gaagatcagt aactgtgttt
     4441 gctttgttgc tgtttgcata agcgttaaca atcaggaata gtccgagtac gcttaaaact
     4501 gaaagtgctt ttttcatttt tatttataat tttctgctaa aatagtattt taagttctat
     4561 aatacaactg accaaagtca ctgttgcgac tttttcatta aataaccatc tgttaaatca
     4621 cttttctgcc taccagtcag agtgtttttt taaaacacca tccttcaaca ccggaactac
     4681 aagagtttga ttaggtgtca ggcaatacaa tacgtcttca tcaattttaa gcctttccaa
     4741 tcgtgtacga tgtgaagagt cttttaaaaa atcgtacatg tcatgctttg caaggttata
     4801 aagattcatg gctgcaatag cagaatcacg atgctctgaa agttgaaaat gtggaagaag
     4861 tttttgcgat aatgcacctg caaacagtgt gtcttcaaga ttaaaagtat ttttccatcc
     4921 tgcacaaagg caaagcacat ctttattctg ctcaataagc catgcagcca gtacatcaag
     4981 attcagaaat gaacctgcaa caattcctgc tgagccattg gcaatgttaa ttgccttagt
     5041 accattggta gtagttagtg caatatcacg accaattatt ttgtcttcca tataactgaa
     5101 aggcgaatta ccaaggtcaa atccttctac cattgagccc tgccgctctg ctcctatcag
     5161 atatcctttt tctttatatt gaaaactttc ttccacagtc attacaggta caatggagcg
     5221 tgcaccatat tgcaacgcca cacaaatact actcgtggca cgcaaaatat caatggcaac
     5281 tactatagat tcctttgtgt tgtactgata gaaaagtgcc ggtgttaaac aaacttcaac
     5341 tataggtttg cctgcttcag acatggatta ttttttaata aacggaatct tgacaactgc
     5401 ggctttaatc agtttttctc tgattttaat aaaaatctct gttccttcgg cagattgatc
     5461 tttcgataca tagcccattc cgatggcttt gtttaatgtt ggcgactgtg ttcctgatgt
     5521 aacacgacca atcttctgac ctgatgcatt acaaatttcg taatcgtgtc ttggaattcc
     5581 acgatcaatc atttcaaagc ctaccaattt gtttgctatc ccctgttctt tctgttgttt
     5641 taatgcatca gagtttgtaa aattctttgt gaatttagta atccagccta agcctgcttc
     5701 taatggtgat gtgttgtcat caatgtcatt tccataaagg caaaatccca tttcaagtcg
     5761 caacgtatca cgcgaaccaa gacctgcagg ttttatgtcg aatggttgac ccgcctgcat
     5821 aacgtcattc cagatttttt ctgcatactg attttcgaaa tacaattcaa aaccacctgc
     5881 accggtgtag cctgtattgg agataagcac attctgacaa cctgcaaact ctcctttggc
     5941 aaaattgtaa tatggaatgt cggccaatgc tatggatgtt agtttctgta atgtttccat
     6001 tgccttaggc ccctgcacgg caagtaaaga agttttatcg gagatgtcgt gcatttcgac
     6061 ttttttgttg ttatgctttt gaagccaatt ccagtctttt tcaatattcg atgcattaac
     6121 aactaacatg aatgtttttt catcaataca atacactatt aaatcgtcaa ttattccacc
     6181 agtttcattg gggaaatagc tgtactgtgc tttgccggct gacaatttgg aagcatcatt
     6241 gcttgtcaca cgctgaagaa gatcaagtgc atcatcacct tttaaaataa actctcccat
     6301 gtgagagaca tcaaacatgc caactgcatt acgaacagtt tcatgctcgt cattaatccc
     6361 cgtatattgt acaggcatca ggtagcctgc aaagggcacc attttagcgc ctgccttttg
     6421 atgtatagct gttaaagctg tgtgttttaa ggtggtattc tcttccatac tattttaaaa
     6481 tgtgcggcaa atctacatgt tttactgctt gttcaatatg attcatgtta tttcaatatc
     6541 aggtttttat ctttacacca atttccacaa cggttttata tacagatgaa actactaact
     6601 tcagaacaat tccgtatggc cgatgcctac acaataaaaa ctgagcctat aagttctgtt
     6661 gatttaatgg agagagcagc tatttgttgc agtgatgaaa tagcaaagtt tacagatgaa
     6721 aaagaccata tttgtattct ctgcggaccc ggaaacaatg gaggtgatgg acttgccatt
     6781 gccagaatat tggccgatgc aggaaaaaat gtttctgttt tacttacaga caatagcaac
     6841 aagctaagtc ccgatgcaga agcaaatttt ctgcgcttgc agaaaacttc tgttaaaata
     6901 ttttacctca ctgatgcaaa cctacctttt aaaaatacaa ctttttgggt tgatgcaatt
     6961 tttgggacag gattaaataa ggcagtagaa aaaaatattg ctgatttaat tcaccaagtg
     7021 aattcaacca gaactcctgt aatttccatt gatttaccct cgggtttatt tgccgacaaa
     7081 catacgccaa cgaatgttgc tgttattcat gcatcaacaa cattaacatt tcagcgactt
     7141 aaacgaagta tgctttatgc agaaaacgcc cacaggattg gcgaagttaa agttcttgat
     7201 attggacttg atgaacatta tatggaatca ttacctacaa ctcaagaact ggttgacagg
     7261 agtttgataa ataaaatact caagccgaga aaacgatttt cacataaagg caattttggt
     7321 catgctttat tgattggcgg aagctatgga aaagcgggtg caataattct atcagcaaaa
     7381 gcatgtgtta aaagtggtgc agggttgaca acagttcata ccgtaaaaac aacaggagcg
     7441 acactacaat ctgcaattcc ggaagcaatg ttgagcactt gtgaaggtga caaggtatgt
     7501 gacttaccgg atttagcacc ttacaatgca attgctgcag gccccggctt gggtattaac
     7561 gaacaaacct caagcatgtt gaaactactt cttcaaaaag caaaaaaaac attggtactt
     7621 gatgctgatg cattaaatat aatttcacaa aataaaaatc tgatagacaa tcttccggaa
     7681 aacaccattc ttactcctca taaaaaagaa tttgaaagac tattcggaac atttgagaat
     7741 gattttcaaa gaaatgattt tcaaattgct gcttcagtaa aacacaaaat cattattgtc
     7801 ttaaaaggtg cttacacttc tatttctact cctgagggaa aatgtttctt caatacaaca
     7861 ggcaatgcag ggctggcaaa aggcggcagt ggcgacacat taaccggcat tattactgca
     7921 ttgcttgcac aaggcttaaa cgcaaccgat gctgcaattg cagcagttta tattcatggt
     7981 cttgcagcag attgtgctgt taaaaaaatt cacccttact ctttaaccgc aagtgatgta
     8041 acagaatttc tttctgaagc ttttatacag acggtgtaat tacagcaggc tcaatccagt
     8101 cgccattatc gcgaatcaat tcaatgagtg catcaacagc aagtgcggca tcaatatttc
     8161 tgcggacgac ttctttgcct ttatagagtg ttatttttcc tgcaccggaa cctacatagc
     8221 cataatctgc atcggccatt tcaccgggac catttacaat acatcccata atacctatct
     8281 taatgccttt gagatgatgt gttcgtgcac ggattttagc agtggtttcc tgcaggtcga
     8341 ataatgttcg tccgcatgaa gggcaactga tatattctgt ttttgaaatg cgtgttcgtg
     8401 cagcttgtaa aattccaaat gctgtttgat taatatctgc cggactaact aattctgaac
     8461 aaagccaaac accatcaccc ataccatcca ataacaatgc acctgcatca gtggaacaat
     8521 gtaaacgaaa tgtttctaaa tcagctttag cataatcacg ctttatgatg acaggtaaag
     8581 tatttttctg ctgcatcata tcaacaaaca cattacgtaa atccggcatt gcatgatcgt
     8641 tagatgtttt taagacaaga acagcatcag aaagtttatc aacttgcaac tgttgtttaa
     8701 aaaaatcttc agtgtttaat tcaataaact ttatggcggc agtgcatttt aatgcatcat
     8761 tcagctcatt cacattaaac aacggataag ttctttcatc atcggaatgt tgtagccaca
     8821 ttttattgtt gtaaatgaca cccaatgttc cgggcaatgc aaatgaaata tgttgattac
     8881 ctacataaat aaaatcacat gcctgatcgc tgatatgcca tttatccaat gactgtgaat
     8941 aattatatcc aacaggaaat aatgaagcag gagaaatatt ttgcttgcct gaaaaatctg
     9001 caatcacaac cgggacatga tttcctccaa cattcaaaac agcttttgtt agtcgtttgc
     9061 tgtaactgaa tggagaataa ggtagtttat gatggttaat aggttttatt gtttggtgtg
     9121 atgtgcgatt attataacgt gacaccaatt tttgtgcaac aggaatttca agttccggat
     9181 cttcggttaa cgaaactctt atggtatcgc caataccatc ttcgagtaat gtgcctatac
     9241 ctgatgctga ttttattcgt ccgtcttcag cctcgcctgc ctctgttaca ccaagatgca
     9301 aaggaaaaag agcaggcaaa gtgttttgca tattattcaa atccataaac tctgcacgca
     9361 ttttagcaat caaaagtcta tatgcctgaa ccattacctg aggattactt gctttcatag
     9421 aaagtacgat gctataataa ccttcatcca tacaaattcg caaaaactca agagcagact
     9481 ccaccattcc caaaggtgta tcgccataac gactcataat acgatcgctc aaagaaccat
     9541 gattggtacc aatgcgcatt gcagtaccat actctttaca aatctttact aatggaataa
     9601 accgttcgcg gattcgctct aactcttcag cataagttac atcagtatat tcaatagtgt
     9661 cgaacttttt cttgtcagca tagttgccgg gattcaccct tactttctcc acaatacgag
     9721 ctgcagtttc tgcggcatta ggtgtaaaat ggatatcggc aatcagaggt gttttatagc
     9781 ctctcttgcg aagttcgtct ttaatattct gaagattacg tgcctcattg atgcttggtg
     9841 cagtgattcg cacaaattca caacctgcca caatcatcct gatgctttgt tcaactgtag
     9901 ccttggtgtc aagagtgtct gtagtggtca tactttgaac acgaatggga tgattagctc
     9961 ccattgctac atcgccaatc tttacctcaa gtgtttttag tctttggtaa gaagtaaggc
    10021 tgttacaata tagttggaaa atattttgat ctgtgagggc gctatccatt tttaaaataa
    10081 atatttagta tgcaaatatc ggaattgtat ttctttagat tagcataacc gagtaaagac
    10141 attaaagttt taccacccgt ttttgaagca cctttttgtc agattccagc agcagataat
    10201 aagtgccggg tgcaagattt ttaggataaa ttgtaagttg atcaagagcc tgcctttgct
    10261 tgctgtgata aacgacctta ccaacaatat tcactaattt aatatcgtac tttctgtata
    10321 aaaaatcttc agagtgaatg catatctgat ctgtgaaagg attcggataa aggcttacat
    10381 agtctttttt gattacttca ctttcaccaa caacaactac agaatcgcag tcaaccaagg
    10441 tgttgggctg atacaaacct aatgttgaat cgttgtaaca tctaaaagca gttatctgag
    10501 gatcgcataa attgaattgt gtaagtaaaa aattcaaact gccaatacct tcaataatag
    10561 gcccttcaaa aacaaatgga atgtgagatg aatcggcata agaaatgtaa aatatttttc
    10621 ggggatgatt gttgatatta accatgttca ctgagtcaac acgaatacgc acagtgtctg
    10681 taagtggtgc cggtggcggt tgattaatgg tgtttgatac tatgttccat atatcacccg
    10741 gctgtgcatt aaaatcgtaa agcatggtaa aggtgttgag attgtagaga aaacgataaa
    10801 cagtatcgtt actctgataa acaggcaaat aattaaagcc aacacaagta aaaggagcaa
    10861 tctgtaggtt agtgcatgta atgccattaa tgacagtagt tccagttgca tttacttcaa
    10921 cagggccgca ggaataagga aatgtaactg tacattgcgt gtagtgccac tgtgcaccaa
    10981 caggtgcaaa ctgtgcttta gctacaaaat gactacaagc tataagcaat acaaaaatta
    11041 tctttttcat tgcagtgcta agatacttaa aacaaagtga caaacaaata ttcaaccaac
    11101 taccttttca aatatttcga gatagtattt tttgtttaca tgaagtgaat actctttctc
    11161 aaccttcatt cttccattca tgccaattcg ttttctctct tcagggtgat ttatcagata
    11221 ttcaattttt tcaatccact gttcatctgt tcttaccaga aaccctgact cgccatcatc
    11281 aattattctg aagtttgtcc cgattccggt tgcaatggtt ggaattccca aagccatata
    11341 ttgcaatgcc ttgcctccac tcttcccata aacccattgt tcatcaggaa gtggatatag
    11401 gccaatatca attttctgaa ggtctttaac ctctgttgcc gattgccagg gaatggtttc
    11461 acaatcgaag ccattaaact taaacgttgt gtctcctatt accaggattc tgatattact
    11521 gcgaccggca aatttcctca acacttcttc caacaaaaat aaataacggc ttgtactgtg
    11581 actgccactc catcctattg ttataacatc attgttgctg tatttatttg caggaagata
    11641 aacatctgtg ttgatagcta ctgaaatatc agtaattgca ttgttatact ttctggcaaa
    11701 ctcctcatta gaaggtgttg aaacaacaat gtgcttagcc ttcttcatca ggaaagtaat
    11761 ttttcttgta ccttttaata ttttaataaa cttattggct ttagaagaat gaccaagaaa
    11821 aaccatgtca tcaatatcat aaatcatatt agggtttagc agcacaaaaa gccattcaaa
    11881 cagcggcaat ccgaatggtg ttacccacaa aaaaatatac actgcatcat aacgccataa
    11941 cgtaaacagt tgcaaaaagc gttttatgta gccaaacaaa gtccagaata ttttctctgg
    12001 aacatttccg ggtttataaa caatgctgtt aaaacgatag gtcatgaaag atgaaacatg
    12061 tatatcatat ccttcttttc tgaacaaatt cagatactgc tcataacgca aacgttgccc
    12121 cggtgcagta tcttcgggat aagggcaaat cagtagaatt ttcttcttat tgtggctcat
    12181 aaatttttgt tgcctgcaaa aacaaggtca gcaaattgtc tgcgaaaata aagatgattt
    12241 tgtaattatt gtttttgcat tttttcataa gcctgtgcat atagtttaac tccgctttca
    12301 agtgcaaaaa ccggacgaga ttgttctctg atattctctc ttggaacctg aatcatttca
    12361 atagctcttt ttgctacaga ctgcatttca tcttctgtaa aaccatgaat tattacacct
    12421 gtctttgttt cattaatgat acgctcaaca tcgccaacac cactgttgca aatcaatgga
    12481 actcctgcag caagcatctc ccccatcttt gttggcgaac tgcttagttt tgaaaagcaa
    12541 ggtttgataa atgaaattcc cagatgcgca attgataaat attttggcac ctgctgtcgc
    12601 gttgcactca ctaccttgat taaatctgta tttattccca atttttttgc cgaatcgaaa
    12661 accattgatg gaggttcagg tgtcataata aacaggcgga aggaattatc ctgaaaatgc
    12721 aactgcttaa acatagccat catctcatca aacatatacc atgttcctat tgaaccgagg
    12781 taacacaata tcttgtttga tgcttcaaaa cctagctgct taaccaatgc caaatcacgc
    12841 ggtaaaggtt tgaacaattc ataatctgag caacatggaa taatagagaa cttgtttatt
    12901 tgctcgttgc tgacattcat ttttttaatt tcgttcaacc ctgcatgtgt gagactgaca
    12961 ttcaagtctg actcattaaa aaactgcagt tcttttttct tgaaataatt ataaatgatt
    13021 ctgtaaactg gatttttaat attccacaaa tcaccgtcaa tgcgttcatc gggccagaag
    13081 cctcgcatgt caaaaataaa tttggtgcca aacttctttt tcatgtcctg accaatcata
    13141 gcagagagat agcttcgaca atgcactgca tcatacttgt tttctttgtg cagactgtat
    13201 gcttttctgt acatacgaac cacatcaatt actgttgata atacaggggg tctttttgta
    13261 tatggcaaag gataccatcc tatgtttaaa gaatcaagca actttctaat ttctatttct
    13321 ttagctttaa acttatcggg cttttcgcaa ctgataatat caaaatgatg accataacct
    13381 gtgagtcctt tcagataggg aattacctgc gactgaccaa gattatctgt cattccatca
    13441 taagaaatat aaagtacatt cataaagcgt ccaaaaatat tgcatttgaa ccgaattcag
    13501 atgcattaac gaacgaaaga ataaacacta catgctcata tcctgcatac cttcagtatt
    13561 tattttctga tttttcctct tgaatagtga ggtatcgaat ttcccaaagc gatagctgaa
    13621 gtttaatctg aaaatttgtg cgtctcgttt gcgccagcta tcctgtatat aaaaatcaga
    13681 ttctgaatgc acatcattta ctcttgtctt gaaaatatca gaaacattta gcgtaagtga
    13741 tgctgcttta tctttaagaa aatcataacg taaaccagca tcaacaccat aatttggtct
    13801 tatataaccc tgtgccgtac tttgagtgcc gccacccatt ggtccacgtc ctccactatt
    13861 actatttaac tgtaatgctg ttttggattg ataatcacct gaaatactaa cagaaaaatt
    13921 cttaggcagt ttaacattaa ggttgagttt tgcaaaccaa ctaaactgtt caacttttag
    13981 atttgactca atgttttttc cattaatcac actctgataa gcattaatat ttgaagtaat
    14041 gtcgagccaa cctttaatat tattttgtgc tgttaattct aacccataag catagcttgt
    14101 gtttgcattt cggaaggtgc ttaccaatac attacgtccc aaaatagtat ccgtttcata
    14161 gctcagataa cgggtaatta ccccatcagt ttgtttataa taaactgttg ccatcagtgt
    14221 atttttacga ctcatagttt gcatggctgt caattctact gaatgtgtaa actcaggttt
    14281 caatccgggg tttcctttac tgatgtttaa agaatcggaa taatcagaat atggaattat
    14341 ctggaaaaag ttaggtctgt ttattctgcg tgaataattt atctgcaaat cagttttact
    14401 actcagttta taacttgccg atgcacttgg gaacaaacta acaggataat ttgtgctaaa
    14461 cttctgtcct gtttgtgtaa gttcaccttc atagaacgag ctttctgccc ttagtcctgc
    14521 ctgtaatgat aatttttcga atgacttact gtaggttgca taagctgcat aaacctgatc
    14581 tacatattga taatttgctg tctgattagg aattaaaata tattgttgtt gcgagtcgtc
    14641 aaaaataaaa ttctgattta cactgctgta atcgcgcata gcaccacgca gtcctgtttc
    14701 aattttcgac tttttatcaa gcgggaaatc catatcggtt tgtgctgtaa caaatgtgtt
    14761 ttcaccttca atactttgct tctgcaaaat aggattgccg taagcttcgc cagtgcccag
    14821 ataattttgt gtactgtagt ttcctcccat atcactcttt acactgttga aatttatatc
    14881 tgcagtaagt tctttacctt cttgcgggaa aagacgttta taggcaactg tgccacctgt
    14941 atttttaaat tcacgtttgc ttgaagaatt tctgtctgac aaagaagaag atgtatatcc
    15001 cggaaataat gtatccgtct ttgtttgtaa aaactccaat ggtttaaaat atccattcat
    15061 ataacttccc gaaacagtta tggtatttct gttatctaaa aaaatatctg ctcctgaacg
    15121 aataaatcca aaatagccat tgctggaatt ttcattactt tggcttataa aattatttgg
    15181 agtggacccc a
//
LOCUS       DAORQT010000021        14999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_386_length_14999_cov_9.315244, whole genome shotgun sequence.
ACCESSION   DAORQT010000021 DAORQT010000000
VERSION     DAORQT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_386_length_14999_cov_9.315244"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK134_01675"
     CDS             <1..475
                     /locus_tag="PK134_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37594.1"
                     /translation="PSTEHFENQLALQWHKAPLNQFTWIENESIKIGKCALPINFFDQ
                     MREAICFELDVSVAVRIERTLESYGKFNVDLLSESTMKISKRLGGLRLKTALEALEQK
                     DLKLWCSIVMEYYDKTYQHSHDKREKNEIIKVDAEGLDKAVIANEMIKLSKQVVL"
     gene            472..1506
                     /gene="selD"
                     /locus_tag="PK134_01680"
     CDS             472..1506
                     /gene="selD"
                     /locus_tag="PK134_01680"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRB37595.1"
                     /translation="MSEVVKLTQFSRQGGCGCKIAPEKLSEIIHDKESVVFKQLLVGN
                     AESDDAAVFDLGNGTSVISTVDFFMPIVDDPFLFGSIAAANAISDVYAMGGTPVMAVA
                     ILGFPINKIPLSVAKEIKAGAEKVCNDSGIPLAGGHSVDSLEPMFGLAVTGIIPTENV
                     KRNSTAKEGDYLLLTKPIGAGIITAALKRGVISESHLEQAMKQMTTVNTVGKELGRLK
                     CVNAMTDVTGFGLAGHLKEMCTPGNLSVEVYFDKLPVMDGAKEYISKFIYPDMTTKVY
                     SSIAAQCSELNSEQLFISCDPQTNGGLLVAVSPEGLNEVLLVFDKYNIPYNTPVGRFL
                     SRQEKMINIL"
     gene            1647..3557
                     /gene="acs"
                     /locus_tag="PK134_01685"
     CDS             1647..3557
                     /gene="acs"
                     /locus_tag="PK134_01685"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRB37596.1"
                     /translation="MYPYQITSAEQYTSVYRNSIENPEKFWANIAQYFTWQKAWDKVL
                     DADFEKAEVKWFAGAKLNITENCLDRHIEKLASQPAIIWEPNDPTEHHRVLTYKELLF
                     KVKQFANVLKNNGVKKGDRVCIYMGMIPELAIAVLACARIGAIHSVIFGGFSARSIAD
                     RLQDADARFIVTCDGAFRGAKDIPLKAIIDDALESCPFVQRVIVCTRTHTPVSMIKGR
                     DVWWEDEIKIVETQGNPDCIAEVMDAEDVLFILYTSGSTGKPKGVVHTVAGYMVWTNY
                     TFVNVFQYKPGQIYFCTADIGWITGHSYLLYGPLSAGATTMMFEGIPTFPDAGRFWDI
                     TQKHKVDILYTAPTAIRSLMAYGTSYLEGKDLSSLKVLGTVGEPINEEAWYWYYENVG
                     KSKCQIVDTWWQTETGGIMISNLAGITPHKPTYATLPMPGVLPCLVDETGNELIDNGV
                     SGNLCIKQAWPGMIRTTYGDHERCRQTYFSTYKNMYFTGDGCLRDKDGNYRITGRVDD
                     VLNVSGHRIGTAEVENAINMHQGVVESAVVGFPHDIKGQGIYAFVIFNGMHVDEEHTR
                     QDVIQTVSRIIGAIAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGELNNLGDTTTLLD
                     PGVVEEIKSGRL"
     gene            3660..6308
                     /locus_tag="PK134_01690"
     CDS             3660..6308
                     /locus_tag="PK134_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37597.1"
                     /translation="MNRIVVSSIISIVIIGLMAVGYYYFSAYREKFSATEDALPSDAA
                     IVIRGSMGDLYASLEKVGLWNERDSLTVMYRFKKEMHTLLSNTTSMFDVADLLESPQT
                     TIAVLATGARNAELLYLVPSRKELNIDALQKALTGQLSENKSRTFAGVSIYELQFSNF
                     SSSFTFAFANGIFIGSFNAGLVEDAIRQQATGKPFGGNVSAQKLYKEIAVSSGTILGL
                     NYPALKKLFSVAISSDNVIELNNLEKWTEWSFHNLTFDGNSINANGSVLVSDSTQQIA
                     DFGNVRSSTDLLWSVLPDDVSALQIFSFKDALIWSDKNQRRNADNEVIINYIKQVSVT
                     ETKLRVEIRKDFSTIVGEIGAIAINGNPTSLLENNSVAILQLKDQKKSLTQLLALSKK
                     LSKNKDVIAVEKFRNHEIRFLNCDGLLPAMFGNAFNVIRKNYFTTHNGFVIFANKPSQ
                     LRGFIDDVEDNEMLNKNEVYQQNKSVLSESLSYALFLRPTNLNSYFQSVANSNTLKAL
                     AASDLMYKFSSFYYYIQPGISGKVDCNFRIVIQQQKQQKLSEPKWSFFTDSAIHCGPY
                     IFTSDNGISILFQDSTKSLICLDGSGNVSWKTTISGFINGEISQTINDQSGMIQFICN
                     TNDSMYMFNQNGVMPAHYPFKLPVHSRYGIVASPNTNVLFVAGENNLMYAFDPGSRPS
                     QNWNTVQFEGNLFNASIDALGRFVYIMDGDKLRLYSTDGKLILSKPSTEFRVNSMQWV
                     NDSVPSLFMLNNDGKIVVWNIDGTYKIASSDSTFDFAETCGSGTARIWYTIKGRELNI
                     LDYTFKAVENVDLSKVEFNNMQLFNTGNEILCILKGSENITIYRGDLKIVPQVIPAAD
                     FAVKTFQGKTYLISRSGNGKLTFYVL"
     gene            complement(6305..>7104)
                     /locus_tag="PK134_01695"
     CDS             complement(6305..>7104)
                     /locus_tag="PK134_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HRB37598.1"
                     /translation="NIDNIGACASINSCVNDISHWIMMQLNKGKYENKEVIPSAVINT
                     TQNSYMIVRETGSARMPSNHFQNYGLGWKSEDMYGYKIVSHDGGANGFVTNTTLLPEL
                     NAGFVILTNTDANWFYGALRQQLINYFANQAYTNVSENYYGIYSKNNITESEEIQNLK
                     KQANAGVRLNVADVALVGKYSNEVYGEIEIKKEKSGLTMCFSHHPKLRGALKPLSEKS
                     LLCTFNDPTYGIHSFPFEVVNGKVNNITVKVNDFIDYQYYKFTKQNN"
     assembly_gap    7105..7148
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7149..7836)
                     /locus_tag="PK134_01700"
     CDS             complement(<7149..7836)
                     /locus_tag="PK134_01700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRB37599.1"
                     /translation="MKKLLGLFLLTVLTTFQTFAQGDFLKDSLDNYINREMQRWQIPG
                     VAIAIVKDGKVITTKGYGLRETGKPDKVDEFTLFQIASNTKAFTGTALALLDYQKRIS
                     LDDKVTKYLPWFQLKDPCATEMVTIKDILCHRIGFYTFQSDFLNWDCNMTTDALIKNM
                     RNVQPVNEFRAKYGYCNMGYVTAGEVIKAVTDTSWQDFFDYHFFKPLQMNRSSVFHQK
                     IVTDANAAKPY"
     gene            8039..>8276
                     /locus_tag="PK134_01705"
     CDS             8039..>8276
                     /locus_tag="PK134_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRB37600.1"
                     /translation="MFNTEKYKKICEELLPYKANLIAVTKTKPVTDIEAAIQNGQMLF
                     GENYVQELVEKVPVLPKNIQWHFIGNLQRNKVKQI"
     assembly_gap    8277..8286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8287..8701
                     /locus_tag="PK134_01710"
     CDS             <8287..8701
                     /locus_tag="PK134_01710"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRB37601.1"
                     /translation="IHTVQTVDSRKLLNELNKHAASNNRVIKVLLQVHIAKEEAKFGF
                     SEVEVQMLVDEGVFEQYNNISFNGLMGMATNTDDTMQVRSEFKQLYGLYKSLSDAFDR
                     NESPVLSMGMTSDYKIALDEGSNMIRIGSALFGSR"
     gene            8706..>9180
                     /locus_tag="PK134_01715"
     CDS             8706..>9180
                     /locus_tag="PK134_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB37602.1"
                     /translation="MGLFFALITTLSWSIGIFPFTEASRKLGVNSLNHFRLFLAVALL
                     FATALLINHSEFINLFNEQYVQAWIWFGLSGVVGLALGDYFYFGLYAIMGARIGSIFS
                     TLSPAAALVTSYFFVDERINFIGVIGIFLTMAGVIFISISKSENLSAEEGRYGSDV"
     assembly_gap    9181..9192
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9193..9671
                     /locus_tag="PK134_01720"
     CDS             <9193..9671
                     /locus_tag="PK134_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB37603.1"
                     /translation="VLFGVLAAFCQGVGLVLAKKGFVVLEQEGKFIHPINATFVRMVV
                     SLATLILITTIFRKWKEVLRPLKSNSAGLKYAAVGTFFGPVFGVCMSLYTIAVLEATV
                     AQTIFSLVPVVAALIARYFYKEKFTLASVIATIIAVAGVVLLIRRNEVLECLYKSN"
     gene            9675..12662
                     /locus_tag="PK134_01725"
     CDS             9675..12662
                     /locus_tag="PK134_01725"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRB37604.1"
                     /translation="MRRIVLLVFGFVCIVKIAIGEKPYFQQRVDYKISVSLDDKKNFL
                     SGFEELIYRNNSQDTLRELFFHLWPNAYKYRSSALCYQLVSKGNMSLYFANDEQRGFI
                     DSLNFRVDDKVVSLVYDSINIDICKIVLNKPLLPNDTILITTPFRIKIPDAKFSRLGH
                     VGQSYMISQWYPKPAVYDKDGWHTMPYLSQGEFYSEFGSFLVDITVPANYVVAASGYL
                     KTPSEVEFINKKIKTESNQINFDKQNTFPESESSSKSIRFALDSVHDFAWFADKRFRI
                     VSKVIKLASGHEVECRAYYTPANSYVWHRAADYVAEAVLFYSKYVGEYPYGVCTAVDG
                     TIAAGGGMEYPTITVIGSTGNSMVLNQSIAHEVGHNWFYGILASNERKLPWMDEGINS
                     FYEQLYMIQYYARESAAAYQLTGVPLKIAKTFGADKMSFAGFNKISYLLTARTNDDQS
                     SGLTSEYFSNLNYGADIYSKIPLCFSQLRDFLGHEKYDSCMQTYFNTWKFRHPDEAAI
                     QNVFETTSGKSLGWFFNGMIDSNSKSDAKICKVNTDSGGTDVLLKNRGNLAMPVNVSF
                     YNKEKLIASQWTGVFDGKFKLSSSVSGADKIVLDTYDQSLDLNPFNNSMKTKGVFRKW
                     KPLQLRFLTMLENPERTQLFYLPAVAYNNYNGWMAGVILHNYSLLSKKFEFGIVPMYA
                     FKSKSVAGVATASYQFNIYNGWFDRIIAKISPTLFDEGLSENRSIFKGYFSVPASLSF
                     RIRKSQYKPYSEKYVNIIHHYINQTFSTQNNSSATNEINITQLEFASFNYSKTDPGSL
                     SFILEKGGHYAKLTGTWKKEITLTRRKKDLSLRFFAGTFLDNSDYGGEANFRSSAWRG
                     TDDYSYSGLWFGRNEYDGFWAHQMQRADGAMAAYYPVRTDHWLAALNVSVKTPVSGLR
                     LFADGITFYNANKVSPGATVVRYDAGIMLSIVHDAFEIYWPLIVSKEVRDYYDLNDIK
                     LADRIRFVFNINKLNPVLLRKMR"
     gene            complement(12668..>14999)
                     /locus_tag="PK134_01730"
     CDS             complement(12668..>14999)
                     /locus_tag="PK134_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37605.1"
                     /translation="EGQECSTPIIVCNPNTQVGDPGFQLFGNRCDFPGAGTNCLTRAE
                     RGSAWYQINIASAGNLEFNLIPNDWVGAPSTACTDYDFAVWKIAGTGATTCAGIAAGA
                     TPVKCNYSPLGITGLYSGSNGTAPPAYPGFGGAYQSRLSVAAGEVYILVVSNYSNSIS
                     GFTLNFPASAPVDYTAGGTTAYWTGAESNDWFTAANWGGCNIPSCTVDAVIPLSSANQ
                     PYIGTAGATCKDLKLEPGGTLIMDNNQFLSICGNVQNNGELNIATGSGFLFGNGSVNQ
                     TLNGNLVSPNRFSHITVNKTGGKVDLLQNIEMQGSLALIGPSSRFDAINKTVTLKGSL
                     SNSAGQFNMGNNGNLVLTGTSTQNISNNDTLTNVIVNKSGNTVVLIRDMKIGPTGSLS
                     LIASKIQTTSANEVYVMNDAASAVSAGSSSSYIEGNLRRNVPTIVSPRVYDFPVGNAT
                     KGYQRLNLNLYNGISPAVGSIQASFSDYLTDIWGLGLDAPCLMNYNDTMLNNGYWTLT
                     PANTANGEANITLYNRTYTNAKTNYGVGINPNGAGWMIPPVISGGCVTPPVTAVLRNG
                     VGLSFTSGQSILVGTAQGKVSPLPVELLSFEATAGKQSIFLTWSTASEKNNAGFYLER
                     STDGNNFDNITWVDGHGTTNQVNNYSYEDKNVKEGVLYYYRLKQIDYDGKFEYSFVKT
                     AMIGDLKNVILSAAPNPYRGSTKINYSIPTDGSVVILITDMTGRILKNIDAGMQTKGS
                     YSIPFSAIADGHPSGVYNVTLFFNDKPYRLKISEYR"
BASE COUNT         4675 a         2429 c         2952 g         4877 t
ORIGIN      
        1 accttcaaca gagcattttg aaaaccaatt ggcattgcag tggcataagg ctccattaaa
       61 tcagtttaca tggattgaaa acgaaagtat taaaattgga aaatgtgctt tgccgattaa
      121 tttttttgat caaatgcgag aggcaatatg ttttgaattg gatgttagtg tagctgttag
      181 aattgaacgt actcttgaaa gttatggtaa atttaatgtt gatttattgt ctgagagtac
      241 aatgaagatt tcaaaaagat tggggggact aaggcttaaa acagcattag aggcacttga
      301 acaaaaagat ttaaaattgt ggtgtagtat agttatggaa tattatgata aaacctatca
      361 gcatagccat gataagcgtg agaaaaatga aattattaaa gtagatgctg aaggattgga
      421 taaggcagtg attgcaaatg aaatgattaa actttcaaaa caagttgtgt tatgagtgaa
      481 gtagtgaaac tcacgcagtt tagccggcag ggtggatgcg gatgtaaaat agcacctgaa
      541 aaattatctg agattattca tgataaggag tcggtggtat tcaaacaatt attagttggc
      601 aatgccgaat cggacgatgc agcagttttt gatttaggta atggtacatc tgtcattagt
      661 actgttgatt tttttatgcc tattgttgat gacccttttc tttttggctc cattgctgct
      721 gccaatgcaa ttagtgatgt atatgctatg ggcggaacac ctgtgatggc agttgccatt
      781 cttggtttcc caataaataa aataccactg tctgttgcta aagaaataaa agccggtgca
      841 gaaaaagttt gtaatgattc aggtatccct cttgcaggtg gacacagtgt agatagtctt
      901 gagccaatgt ttggattggc cgttacaggc atcataccga ctgaaaatgt taaacgaaat
      961 tcaacagcaa aagagggtga ttacttactg ctcaccaaac ccataggtgc aggtatcata
     1021 acagctgctt taaagcgtgg tgtcattagc gaaagtcatc ttgagcaagc tatgaaacaa
     1081 atgacaacag taaatactgt tggaaaagaa ttaggaaggt tgaagtgcgt aaatgccatg
     1141 acagacgtta caggttttgg acttgcagga catcttaaag agatgtgcac acccggcaac
     1201 ttatctgtcg aagtttattt tgacaaatta ccggtgatgg atggcgcaaa agaatacatt
     1261 tcaaaattca tttaccctga tatgacaacg aaagtgtatt cgtccattgc tgcacagtgt
     1321 agtgaactca actcagaaca actttttatt tcctgcgatc ctcagaccaa tggcggtttg
     1381 ctcgttgccg tcagtcctga aggacttaat gaagttttat tagtttttga taaatacaat
     1441 attccataca atactcctgt aggcaggttt ttgtcacgac aggaaaagat gataaatata
     1501 ttataacatg ctgtatgtta ttgatgattt aatcggtaca tcacaaaatt tgtcatttta
     1561 cttttaagtg attcatcaat tgaattgaac ctcaagttcc aatttgtatt atattcgtgg
     1621 ctgtgatttt ttaaagtttt attcaaatgt atccatatca gataacatca gcagaacaat
     1681 acactagcgt ttatcgaaat agtatagaaa atcctgaaaa attttgggca aacattgcac
     1741 agtattttac atggcagaaa gcatgggata aagttttaga tgctgatttt gaaaaagcag
     1801 aagtgaaatg gtttgcagga gcaaaactta atattactga aaactgtctt gacaggcaca
     1861 ttgaaaaatt agcatcacaa cctgctatta tctgggagcc caatgaccct acagaacatc
     1921 atcgtgtttt aacttacaaa gagctgctgt ttaaagtaaa acaattcgct aatgttctaa
     1981 aaaataatgg cgtaaaaaaa ggcgaccgtg tttgtatcta tatgggcatg attcctgaac
     2041 tggctattgc agttcttgca tgtgcacgaa ttggcgcaat tcattctgta atttttgggg
     2101 gcttttctgc acgttctatt gccgaccgct tacaggatgc cgatgcccgt tttattgtta
     2161 cctgcgatgg agcttttcgt ggtgcaaagg atattccgtt gaaagcaatc attgacgatg
     2221 cacttgaaag ttgtccgttt gtacaacgtg taatcgtgtg cacacgcaca catactcctg
     2281 tcagtatgat taaaggcaga gatgtatggt gggaagatga aataaaaatt gttgaaacac
     2341 aaggcaaccc cgactgcatt gccgaagtga tggacgctga agatgtgttg tttatacttt
     2401 atacttcagg ttcaacagga aagcctaaag gagtagtaca tacagtagcc ggatatatgg
     2461 tgtggacaaa ctacactttt gtcaatgtgt ttcaatacaa acccggacaa atttatttct
     2521 gtactgctga cataggttgg attacaggac atagttatct gctctatggc cctttaagtg
     2581 caggagcaac aaccatgatg tttgaaggaa tcccgacatt tcctgatgca ggtcgttttt
     2641 gggatattac gcagaaacac aaagtagata ttttatatac agcacctact gctattagaa
     2701 gcttaatggc ttatggaact tcttatctcg aagggaaaga tttaagttca cttaaagtgc
     2761 ttggtactgt tggtgagcca atcaatgaag aggcttggta ttggtactat gagaatgtag
     2821 gcaaaagtaa atgtcaaatt gttgatacct ggtggcaaac tgaaaccggt ggcataatga
     2881 tttcgaatct tgcaggcata acgcctcaca aacctactta tgccacattg ccgatgcccg
     2941 gtgtgcttcc atgtcttgtt gatgaaaccg gcaatgaact tattgataat ggtgtcagcg
     3001 gtaatttatg tattaagcag gcatggcctg gaatgattcg taccacttat ggtgatcatg
     3061 aacgttgccg tcagacttat ttttcgactt ataaaaacat gtactttaca ggtgatggct
     3121 gtctgcgcga taaagatggt aactacagaa ttacaggacg tgttgatgat gtgcttaatg
     3181 tcagtggtca ccgtataggc actgccgaag tagagaatgc aattaacatg catcagggag
     3241 ttgttgaaag tgcagtagta ggttttccac atgatataaa agggcagggt atttatgcct
     3301 ttgttatttt caacggaatg catgttgatg aggaacacac aaggcaagat gtaattcaga
     3361 cagtgtcaag aattattggt gccattgcta agcctgataa aattcagttt gtttcaggac
     3421 ttcctaaaac acgcagcgga aaaattatgc gaagaatatt acgcaaaatt gctgaaggag
     3481 aattaaataa tcttggcgat actacaacac ttctcgatcc gggtgttgtt gaagaaatta
     3541 aatccggaag attgtaaaca gtgcttgttg tcaattaaca ttatcttgta aataacatta
     3601 aacactacta atgtttctcc gagtagtgaa gctatagttt tgactcacaa gttttatttg
     3661 tgaacaggat tgtagtttct tccataattt caattgtcat cataggcctg atggctgtag
     3721 gctattatta tttttctgcc tatagggaaa aattttcagc tacagaagat gcattgccta
     3781 gtgatgctgc catagtaatc agaggtagta tgggcgattt gtatgcttct ttagaaaaag
     3841 tagggttgtg gaatgaacgg gattcattaa cagtaatgta tcggtttaaa aaagagatgc
     3901 acactttgct gagtaacaca acaagtatgt ttgatgttgc cgatttgctt gagtcaccac
     3961 aaacaaccat tgcagtttta gctacaggag cacgtaatgc agaactgctt tatcttgttc
     4021 cttcacgtaa ggaattaaat attgatgcat tacaaaaggc attaacgggt cagctgagcg
     4081 aaaacaaatc aagaacattt gccggtgtca gcatttacga acttcagttt agtaatttta
     4141 gttcatcatt tacatttgct tttgccaatg gcatttttat tggaagcttt aacgcaggtt
     4201 tggttgaaga tgctatcagg caacaggcaa caggtaagcc ctttggtggt aatgtttcgg
     4261 cacaaaagct gtacaaagaa atagctgttt catcaggaac aatacttggt ctcaattatc
     4321 ctgcattgaa aaaacttttt agtgttgcaa taagttcaga caatgttatt gagttaaata
     4381 atcttgaaaa atggacagaa tggtcttttc ataatctgac atttgatggc aattcaataa
     4441 atgccaatgg ctcagtgctt gtatcagatt caacacaaca aattgcagat tttggaaatg
     4501 tgcgttcttc aactgatttg ttgtggtcag tgctgcctga tgatgtttct gcattacaga
     4561 ttttttcctt taaagatgct ctaatttgga gtgataaaaa tcaaagaagg aatgcagata
     4621 atgaagtaat catcaactat ataaagcaag tatctgtaac agaaacaaag cttagggtgg
     4681 aaataagaaa agatttttcg actatagttg gagaaattgg agctattgcc attaatggta
     4741 atccaacttc attgcttgag aataattcag tggcaatatt gcagctaaag gatcagaaaa
     4801 agtcattgac tcaattactg gcactaagca aaaagctttc aaaaaataaa gacgttattg
     4861 ctgtagagaa atttcgtaat catgaaatac gctttttaaa ttgtgatgga ctcttaccgg
     4921 caatgtttgg aaatgctttt aatgttatca gaaaaaatta ttttacaact cataacggtt
     4981 ttgtaatatt tgctaataag ccaagtcagt tacgtgggtt tattgatgat gttgaagata
     5041 atgaaatgct taataagaat gaagtgtatc agcagaataa gtcagtttta tctgaaagct
     5101 tgtcttatgc tttattctta agaccaacta atcttaattc atattttcaa tctgttgcaa
     5161 acagtaatac attaaaagca ttggcggcat cagatctgat gtataagttc agtagttttt
     5221 attattacat tcaaccgggt atttccggga aagttgactg taactttaga attgttatac
     5281 agcaacaaaa acaacaaaaa ttgtctgagc caaagtggtc gttttttaca gattctgcaa
     5341 tacactgcgg accatatatt tttaccagcg acaatggcat ttcaatcttg tttcaggatt
     5401 caacaaaaag tttaatctgt ctggatggca gtggtaatgt aagttggaaa acaactataa
     5461 gcggatttat taacggagaa atttctcaaa ctataaatga ccagtcaggt atgatacaat
     5521 ttatttgtaa tactaacgac agcatgtata tgtttaatca aaatggtgtt atgccggcac
     5581 attatccgtt taaattgcct gttcattcac gttatggtat tgtagcatca ccaaatacta
     5641 atgttctttt tgtagcaggt gagaataact taatgtatgc gtttgatccg ggcagcagac
     5701 cttcacaaaa ttggaataca gttcaatttg aaggcaactt atttaatgca tcaattgatg
     5761 cattaggtag gtttgtttac ataatggatg gcgataagtt aagactatat tcaacagatg
     5821 gaaaactgat tttatcgaag ccgtcaactg aatttagagt aaattcaatg caatgggtca
     5881 atgactctgt gccatcactc tttatgctga ataatgatgg aaaaattgtt gtttggaata
     5941 tagatggtac ttataaaatt gcatcaagcg acagtacttt tgattttgcg gaaacatgtg
     6001 gttcaggtac agcaagaatt tggtacacta ttaaaggtag ggaattgaat atattggatt
     6061 acacattcaa agcagttgaa aatgtagatt taagtaaagt tgagtttaac aacatgcaac
     6121 tttttaatac aggaaatgaa attttatgta tccttaaagg atctgaaaat attacaattt
     6181 acagaggtga tttaaaaata gtaccacagg taattcctgc agcggatttt gctgttaaaa
     6241 catttcaggg gaaaacctat ttgatttctc gttcaggaaa tggtaaactt accttttatg
     6301 tactttaatt attttgtttg gtaaacttgt aatactgata gtcaataaaa tcatttactt
     6361 taactgtgat attatttacc ttcccattaa caacttcaaa gggaaaagag tgaataccat
     6421 aggtagggtc gttaaaagta cagagtaatg acttttcaga gagtggttta agtgcaccac
     6481 gtaattttgg atgatgtgaa aaacacattg taaggcctga tttttctttt ttgatttcta
     6541 tttctccata tacctcattg gagtattttc caaccaatgc aacatctgca acattaagac
     6601 gaactcctgc attggcctgc ttttttaaat tctgtatttc ttccgactca gtaatgttgt
     6661 tttttgaata aatgccgtag tagttttcgc taacatttgt gtatgcttga tttgcaaaat
     6721 aattaatcag ttgctgacgt aatgctccgt aaaaccaatt agcatcagta ttagtcagaa
     6781 tcacaaaacc tgcattcagt tcaggtaata aagtggtgtt ggtcacaaag ccattggcac
     6841 cgccatcatg cgatacaatt ttgtaaccat acatatcttc tgacttccat ccaaggccgt
     6901 aattttgaaa gtgatttgaa ggcatacgtg cagagcccgt ctctcttaca atcatataag
     6961 aattttgagt agtgttaatc actgcagaag gaatcacttc tttgttttca tatttacctt
     7021 tgtttaattg catcattatc caatgtgaga tatcgttaac gcatgagttt attgatgcgc
     7081 aggcaccaat gttgtcaatg tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnta tatggttttg ccgcattcgc atctgttaca attttttgat gaaatacact
     7201 gctgcgattc atttgtaacg gtttaaaaaa atgataatcg aaaaaatcct gccatgaagt
     7261 atcagtaact gctttgataa cctcgcctgc tgttacatag cccatattac aatatccata
     7321 cttggcacga aactcattta cgggctgaac atttctcata ttttttatta atgcatcagt
     7381 tgtcatattg caatcccaat tcaaaaaatc actttgaaag gtataaaaac caattctatg
     7441 gcagaggata tctttaattg taaccatttc tgttgcacat ggatctttaa gttgaaacca
     7501 ggggagatat ttagttactt tatcatctaa tgaaatccgc ttttgataat ctaagagtgc
     7561 caatgcagtt cctgtaaaag ccttcgtatt gctggcaatt tgaaataaag taaactcatc
     7621 taccttgtcc ggtttccctg tttctctcaa tccatagcct ttcgtggtga ttacctttcc
     7681 atcttttaca atggcaatgg caacacctgg tatttgccat cgttgcattt cacgattaat
     7741 gtagttgtca agactgtctt ttaaaaaatc gccttgtgca aatgtttgaa aggtggttaa
     7801 cactgttaac agaaaaagac ctaagagttt tttcatttcg ataaatttaa tgtatcaaat
     7861 gtatggaatt ttaaatatac aagcacctgc attgtagttt atccggaata ccggttttac
     7921 tttctgagag caagctatgc caatgggtgt ggcttgctta tttttgtaga aagatttttc
     7981 taaactataa atgagtctca gataaattac gaattttacc gttgattatc tgactcagat
     8041 gtttaataca gagaaataca aaaaaatatg tgaagagttg ttgccttata aagccaacct
     8101 tattgcagtt accaaaacta aacctgttac agatattgag gctgccattc aaaatggaca
     8161 aatgcttttt ggcgagaatt atgtgcagga actggtggag aaggtgcctg tcttaccgaa
     8221 aaatattcaa tggcatttta tcggcaatct acaacgcaat aaagtaaaac agattgnnnn
     8281 nnnnnntatt catactgtac aaacggttga tagtagaaaa cttttaaatg aattaaataa
     8341 acatgctgca tcaaataaca gagtaattaa agtgctttta caggtacata ttgcaaaaga
     8401 agaagcaaag tttgggttct ctgaagttga agtgcaaatg ttggttgatg aaggggtgtt
     8461 tgaacaatac aacaatatta gttttaatgg attgatgggg atggcaacta ataccgatga
     8521 tactatgcaa gtgcgttctg agttcaagca gttgtatgga ttgtataaat cactttccga
     8581 tgcatttgac agaaatgagt ctccggtatt aagtatgggc atgacatccg attataaaat
     8641 tgcattagat gaaggaagta atatgattcg aataggttca gcattgtttg gaagcagata
     8701 gttatatggg attgttcttt gcacttatta caacactttc ctggtctatc ggtatttttc
     8761 catttacaga agccagccga aaacttggag taaattcact taatcatttc agattatttc
     8821 ttgctgtagc tttacttttc gcaacagcat tattaataaa tcattcagag tttatcaatc
     8881 ttttcaatga gcagtatgtt caggcttgga tttggtttgg actttcaggt gttgttgggt
     8941 tagcattagg tgactatttt tattttggac tctatgcaat aatgggagcc aggataggaa
     9001 gtattttttc tacactttca ccggcagcag cattagtaac atcttatttt tttgttgacg
     9061 aacgtattaa ttttattggt gtaataggaa tatttctcac catggcagga gtaatattta
     9121 tttctatttc aaaaagtgaa aacctgtcag cagaagaagg caggtatgga agtgacgtta
     9181 nnnnnnnnnn nngtgttttg tttggtgttc ttgcagcttt ttgtcaggga gttggattgg
     9241 tacttgccaa gaaaggtttt gttgtattag aacaggaagg gaaatttatt catccaatta
     9301 atgcaacatt tgtcagaatg gttgtttcgt tggcaacttt aattttgata actacaatat
     9361 ttagaaaatg gaaggaggtt ttgcgaccac taaaatcaaa tagtgcaggg ctaaaatatg
     9421 cagcagtagg aacttttttc ggacctgttt ttggtgtctg tatgtcgctt tatacaattg
     9481 ctgtacttga agccacagtt gcacaaacaa ttttttcatt agtacctgtt gtggcagcat
     9541 taatagcccg ttatttctat aaagaaaaat ttactttagc atctgtaatt gcaacaataa
     9601 ttgctgttgc cggagtggtg ttattgataa ggcgaaatga agtccttgaa tgtttgtata
     9661 aatctaattg agtgatgaga agaatagttt tacttgtttt tggttttgtt tgtattgtta
     9721 agattgcaat tggtgaaaaa ccttatttcc agcaacgtgt agattataaa atctcagttt
     9781 cattggatga taagaaaaat tttctttccg gattcgaaga attgatttat cgtaataatt
     9841 cgcaagatac tttacgtgag ctgttttttc acctttggcc gaatgcttat aaatacagga
     9901 gttctgcatt gtgttatcaa ttggtaagta aggggaatat gagtctgtat tttgcaaatg
     9961 atgagcaaag aggatttatt gatagtctga attttagagt tgatgataaa gttgtgtcat
    10021 tagtttatga ttccattaat attgatatat gtaaaattgt tctgaataaa ccattattgc
    10081 ctaacgacac aatcttaatt acaacaccat tcagaataaa aattcctgat gcaaagtttt
    10141 caagacttgg tcatgttgga cagtcttaca tgatttcgca atggtatcct aagcccgcag
    10201 tttatgataa agatggatgg catactatgc cttatttatc tcagggagaa ttttattctg
    10261 agttcggttc ttttctggtg gatataactg tgcctgcaaa ttatgttgta gcagcttcag
    10321 gctatttaaa aactccttca gaagttgagt ttattaataa aaaaataaag acagaaagca
    10381 atcaaattaa ttttgataaa caaaatactt ttcctgaatc agaaagttca agtaagtcaa
    10441 ttcgttttgc acttgacagc gtgcatgatt ttgcctggtt tgcagataag cgattccgta
    10501 ttgtatcaaa agttataaag ttagcttccg gacatgaggt ggaatgtcgt gcatattata
    10561 cacctgctaa ttcttatgtc tggcatcgtg ctgctgatta tgttgctgaa gcagttttgt
    10621 tttattctaa atatgttgga gaatatcctt atggtgtttg tactgctgtt gatggaacaa
    10681 ttgccgcagg cggaggaatg gaatatccaa caataacggt aattgggagt actggtaata
    10741 gtatggtctt gaatcaaagt attgcccatg aagtggggca taattggttt tatggcatac
    10801 ttgcaagcaa tgaaagaaag ttgccttgga tggatgaagg tataaattcg ttttatgagc
    10861 aattgtatat gatacagtat tatgcaaggg aaagtgctgc agcatatcag ttgactggag
    10921 tgccattaaa aattgcaaag acttttggtg cagataaaat gtcgtttgcg gggtttaata
    10981 agattagcta tttactcaca gccagaacca atgatgacca aagtagtgga cttacaagtg
    11041 agtatttttc gaatctaaac tatggtgctg atatttattc taaaatacct ttatgttttt
    11101 cacaactcag agatttttta gggcatgaga aatatgattc ctgtatgcag acttatttta
    11161 atacttggaa gttcagacac cctgatgaag cagcaattca aaatgtgttt gagactactt
    11221 ccggaaaatc gttaggttgg ttttttaacg gaatgattga tagtaattcg aaatcagatg
    11281 ctaaaatctg taaagtgaat actgattcag gagggactga tgtattgcta aagaatagag
    11341 gaaatcttgc tatgcctgtt aatgtgtctt tttacaataa agaaaagtta attgcctcgc
    11401 aatggacagg agtttttgac ggcaaattca agttgtcaag ttctgtttca ggtgcagata
    11461 aaattgtgtt agatacttat gatcaaagtt tggatttaaa tccatttaat aattcaatga
    11521 aaacaaaagg tgtgtttaga aaatggaaac cattacaact tcgtttttta acaatgctcg
    11581 aaaatcctga aaggacacag ttgttttatc tgcctgctgt tgcatataat aattacaacg
    11641 gatggatggc aggcgttatt ctgcataact attcattgtt gagtaaaaaa tttgaatttg
    11701 gaattgtacc aatgtatgca ttcaaatcaa aatctgttgc aggtgttgca actgcaagtt
    11761 atcagtttaa tatttacaat ggttggtttg acagaataat tgctaagata tcacctacac
    11821 tatttgatga ggggttatct gaaaatagaa gtatctttaa aggatacttt tctgttcctg
    11881 catcattgag ttttagaatc cgtaagtcac agtataagcc atattcagaa aagtacgtaa
    11941 acattattca tcattatata aaccaaacat tcagtacaca aaataattcg tcagcaacca
    12001 atgaaatcaa tattacacaa cttgagtttg caagttttaa ttacagtaaa acagaccccg
    12061 gttcattgtc atttattctc gaaaaaggag gtcactatgc taagttaaca ggaacatgga
    12121 aaaaggaaat aacattaacg cggagaaaaa aagatttgtc attgcgtttt tttgcaggaa
    12181 catttttaga taattctgat tatggaggag aagctaattt ccgttcaagt gcatggcgcg
    12241 gtactgatga ttattcttat tcaggtttgt ggtttggcag gaatgaatac gatggattct
    12301 gggcgcatca aatgcaaaga gcagatggtg caatggcagc atattatcct gtccgtacag
    12361 atcattggct tgctgcatta aatgtttcag ttaaaacacc ggtatcaggc ttgagattat
    12421 ttgctgatgg aattacattt tataatgcaa acaaagtaag tcctggtgca acggtagttc
    12481 gttacgatgc cggtatcatg ttaagtatag ttcatgatgc atttgagatt tattggccac
    12541 tgattgtttc aaaggaagta agagattact atgacttgaa tgatataaaa ctagcagaca
    12601 ggatccggtt tgtttttaat attaacaaac tgaatcctgt cttgctacgc aaaatgcgct
    12661 gagattttta tctgtattcc gaaattttca atctatacgg tttatcattg aagaatagtg
    12721 ttacattata aactcccgaa ggatgtccat cagcaatagc actgaacgga atgctgtagc
    12781 tgcctttagt ttgcattcct gcatcaatat ttttcaatat tctacctgtc atatcagtaa
    12841 ttagaattac aacactgcca tcagttggaa ttgagtaatt aatttttgtg ctaccacgat
    12901 atggattagg tgcagcactc agtataacat ttttcaagtc accaatcata gcagttttta
    12961 caaaactata ttcaaactta ccgtcataat caatctgctt caatcggtag tagtatagaa
    13021 caccctcttt aacattttta tcttcgtaac tataattgtt tacctgattg gtagttccat
    13081 gtccatcaac ccaggtgata ttgtcgaagt tgttgccatc agtacttctc tccaaataga
    13141 atcctgcatt atttttctca ctagctgttg accaagtcag gaaaatactt tgtttaccag
    13201 ctgttgcttc gaatgaaagt aattctacag gtaatggcga taccttacct tgtgctgtac
    13261 ccactaatat gctttgtccg ctagtgaaac tcaaccctac accatttctt aatacagctg
    13321 ttacaggagg agttacgcat ccgccactaa ttacaggtgg aatcatccat cctgcaccat
    13381 ttggattgat accaacacca taatttgttt tggcatttgt atatgtacga ttgtataagg
    13441 taatattagc ttcgccattt gctgtatttg caggtgttaa tgtccaatag ccattgttta
    13501 acatggtgtc attgtagttc attagacaag gtgcatctaa tcctagtccc caaatgtctg
    13561 ttaaatagtc ggagaatgag gcttgaatac ttcctactgc cggacttatt ccattatata
    13621 aattcaagtt taaacgttga tatccttttg ttgcatttcc aacagggaag tcataaactc
    13681 ttggcgaaac aattgtagga acatttcgtc tgagattacc ttcaatatag gaactactcg
    13741 aacctgcact aactgcagat gcagcatcat tcattacata aacttcgttt gcagaagtgg
    13801 tttgtatttt tgatgctatt agacttaaac ttccggtagg tcctatcttc atatcacgga
    13861 taagaactac agtgtttccg cttttgttta caatcacatt ggtcagcgta tcattgttgc
    13921 tgatgttttg tgtactagta ccggttaata ctaagtttcc attatttccc atattaaact
    13981 gaccggcaga atttgataag cttcctttca atgtaacagt cttgttgatg gcatcaaatc
    14041 ttgagctagg accaattaat gccaaacttc cctgcatttc tatattttga agtaagtcaa
    14101 cttttccacc tgttttgttt acagtgatat gcgagaatct gtttggagaa accaagtttc
    14161 catttaaagt ttgatttacg ctaccatttc cgaaaagaaa ccctgatcct gttgcaatat
    14221 ttaattctcc attattctgc acatttccac aaatagagag gaactgattg ttgtccatta
    14281 ttagagttcc acccggttca agtttaaggt ctttacaagt ggcacctgct gttccaatat
    14341 aaggttgatt tgcagatgaa agtggtatga cagcatctac ggtacaactt ggaatattac
    14401 atccacccca gttagcagca gtaaaccaat cattgctttc tgctccagtc caataggctg
    14461 ttgttccccc tgcagtataa tctacaggag cagaagcagg gaaatttaag gtaaaccctg
    14521 aaatactatt cgaataatta cttacaacta aaatatatac ttcacctgca gctacactaa
    14581 gtcttgattg atatgctccc ccaaaaccgg ggtaagccgg tggtgcagtt ccattggaac
    14641 cactgtataa accagtaatt ccaagaggac tataattaca ttttacaggt gtagcaccag
    14701 cagcaattcc tgcacatgtt gtagcaccgg tacctgcaat tttccataca gcaaagtcat
    14761 aatctgtaca tgctgtactt ggcgcaccca cccagtcatt cggaattaag ttaaattcaa
    14821 gatttcctgc tgaagcaatg ttaatctgat accaagcaga gccacgttct gctcgtgtaa
    14881 gacagttggt tcctgcacca gggaaatcac atcggttccc gaataactga aatcccgggt
    14941 cgccaacctg agtatttgga ttacaaacaa taattggagt actacattcc tgaccttct
//
LOCUS       DAORQT010000022        14305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_421_length_14305_cov_17.086246, whole genome shotgun sequence.
ACCESSION   DAORQT010000022 DAORQT010000000
VERSION     DAORQT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14305
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_14305_cov_17.086246"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PK134_01735"
     CDS             <1..1124
                     /locus_tag="PK134_01735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB37606.1"
                     /translation="DWQTINGENVIFTSFSYLSALEQSKPAGMQFRYVIVYDSKMPVA
                     TLCFQLLDVANKDLDGVLNLKDKGWLLQNLNKSINRLLFKCQTGKVNFLICCGSFFVS
                     GEYGINVINRDALPAVIQLLPEVRQKITDTISNASVCGWMVKDFYNNTKVDQPLLEDD
                     FFELPMDPEMILYIREQWHSFEEYLASLTTKYRVKANSVIRKIEDVDVVALNAAEVLK
                     YGDELIRLYLNIQQKATVQMARVNILYFEKLLTIMPEKFYVKVFFKESKVIAFYCGFH
                     HAGHHEAHFIGIDYSLNKTLSLYQNILYRFIEEAISQRSSNLYYGRTAMEIKSTTGAK
                     AYPLHAYFNLSNTVLNKMVKPVVRRFTPEEWIARNPFRE"
     gene            1249..2985
                     /locus_tag="PK134_01740"
     CDS             1249..2985
                     /locus_tag="PK134_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37607.1"
                     /translation="MIRTIQNTTKAAIILIMFSYIGNPFPAYAKGKTLKATLKGQNVS
                     CFGGSNGSISLDISGGKAPYQITWNDGTSGYAMLDKPAGEYSVSITDSKGSTVSQSIT
                     LTEPYAMTIYSQVTSEQCYARNGKANLLVTGGTQPYQYEWSNQTRQQNLEAVAAGDYE
                     IKVTDKNGCIKQSNITIDRNKVLELNIAKYSPLCNDEASGLIDVEVKGGWSPYTYEWS
                     NGAKTEDQSGLKAGNYSVIVKDNNGCTAKAEVQLKNPETIKVEAKVTEADLNKSNGSI
                     MLQVSGGTGNYTYLWSNMESSQSLNNVEEGVYAVRVDDAHRCSAAAYFTVNEKSPMQV
                     ESVVKHELCNGGKNGSIKLNVKGGVAPYKFQWSNGMNGATISNLTASDYSVLITDANN
                     KVYRNTFTVKQPEPIGIFATVLDESYTGKNDGSIKLQVIGGMPPLKYQWSNGQTVADL
                     ANLSQGTYTVRMKDGNGCAMEHHVLVGINYTDAMLRTAFANEDNAMMEIYPNPFSNNF
                     TMKFSTPVNDIQSIIVYTMDGKIAQQLNPDLKQQQVNEIAVNASKLKPGNYLVKVTTD
                     KKIFTRIVTRTM"
     gene            complement(3077..3949)
                     /locus_tag="PK134_01745"
     CDS             complement(3077..3949)
                     /locus_tag="PK134_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRB37608.1"
                     /translation="MQVLQKTSQYLSLVKFSHTVFALPFAAIGFSLAISQNGLPVFWP
                     LAIKVLVCMITARNAAMAFNRWADRSIDALNPRTALREIPAGIIKANRALWFVIVNAL
                     LFTVTAYLINKLCFFLSPVALFVVLGYSFTKRFTALCHLVLGAGLALAPVGAYLAVTG
                     KFDITPILIGVAVLCWVSGFDIIYALQDEQFDRENKLHSIPSLMGKKQALQVSRILHF
                     LSAGFLALFLYQAHVGLIAWSGLAIFCALLIYQHSLVSENNLSKVNIAFATTNGIASI
                     VFAGMVISDIFLLH"
     gene            4024..4611
                     /locus_tag="PK134_01750"
     CDS             4024..4611
                     /locus_tag="PK134_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HRB37609.1"
                     /translation="MNFLAHLYLSDNNEPLMVGNFIADHLKGNDWKSLPEGIMSGVKV
                     HRFIDHFTDTHPVVEQSKVLLRPYFHKYTPVISDIFYDHFLAAEWDKYSNQPIDEYAR
                     YVYQLMHKNYQFLPARTKEMLVYMENDNWLKNYATLNGMHRALSGMGRRAKFDNNMNQ
                     AAEFLYDNYELFEKHFKEFFPELESAVKKEISTQT"
     gene            complement(4598..6007)
                     /locus_tag="PK134_01755"
     CDS             complement(4598..6007)
                     /locus_tag="PK134_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HRB37610.1"
                     /translation="MRYLDHFEKIAEKRIRDRHKHRYYWNDITFYCNYFSHETFSVLE
                     VGCGTGELLNNIRAAQKTGIDYSPTMIAEAKKQFPQIDFQVMAAENISLEEKYDLIIL
                     SNLTGYLEDVQAVLEQLKKVCHERTKIIVSYHNHLWEPFLRLAENLGLKTKTPQLNWL
                     TSMDVRNLLFLAGFDCYRESRRMLLPINIPLLSPLINKFIAKLPLFRLMCINTYSVAK
                     PLANATEDVNEKYSVSIVIPARNESGNIENGLLRIPKFGKWQEVIFIEGNSTDDTWDS
                     INEVSSKYAASHKIKVGKQEGKGKNDAVRKGFSMAEGDILMILDADLTMPPEDLPKFY
                     EAIASNKGDFINGCRLIYPMEKEAMRFLNMLGNKFFSQAFTWLLEQPFKDTLCGTKVI
                     FRDDYDRLVTNRKFFGDFDPFGDFDLIFGAYKLNLKIQELPIRYRERTYGSTNISRFK
                     HGIILLRMCFFAARKIKFV"
     gene            complement(6020..6676)
                     /locus_tag="PK134_01760"
     CDS             complement(6020..6676)
                     /locus_tag="PK134_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HRB37611.1"
                     /translation="MLDKNAIAKRIAREVKDGMYVNLGIGIPTLVANYIPKGINVTLQ
                     SENGILGMGPFPFEGEEDADLINAGKQTVTLLPGSVIFDSAMSFGMIRAQKVDLTILG
                     AMEVSEEGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCTLP
                     LTGIKCIKRVVTELAVLDVTPQGFKLLERAPGVSVEDIKKATAGKLIVEGDIPEMVID
                     "
     gene            complement(6734..7102)
                     /locus_tag="PK134_01765"
     CDS             complement(6734..7102)
                     /locus_tag="PK134_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRB37612.1"
                     /translation="MSRKFDSPFEDNKILKLTFEFSLKIIHLSEILNSNHQYIIANQI
                     LKSATSIGANAYEAQNAESEPDFIHKFKIAAKETEETYYWLLLCKHAECYPDTTDLLI
                     ELEVIKKIINKIISTSKNKL"
     gene            complement(7144..7851)
                     /locus_tag="PK134_01770"
     CDS             complement(7144..7851)
                     /locus_tag="PK134_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRB37613.1"
                     /translation="MNKVVKNVDEALKDFRDGMTLMVGGFGLCGIPENCIAALVRKGI
                     KGLTCISNNAGVDDFGLGLLLQQKQIKKMIASYVGENAEFERLMLTGEMEVELIPQGT
                     LATRCMAAGYGMPAIFTPAGVGTEVAQGKEVRRFSYNGVEKDYLMEYAFDSDAALVKA
                     WRGDTEGNLVYRSTARNFNPMMAMAGKITIAEVEELVPAGTLDPDQIHTPGIYVHRIF
                     QGEKYEKRIEQRTVRKK"
     gene            complement(8078..9931)
                     /gene="yidC"
                     /locus_tag="PK134_01775"
     CDS             complement(8078..9931)
                     /gene="yidC"
                     /locus_tag="PK134_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRB37614.1"
                     /translation="MDRNSILGVVLIMAVLIGFSIFNQPSKEEQEAAKRKQDSIALVE
                     KKHQDSLKLITKPVAALKADTVAVADTLNNDSLSQTKLTDNFGAFALAAQGESKSITL
                     ESDLLKLKLNSKGGKIDYVELKKYKTGEGKPLVLNNGDSSQFSLSFFSENKMISTGDL
                     YFDLPAKGITVTGKDSQSVSIKLNAGEGRYIEYVYSLKGDDYRVGFKINFVNMGSLVA
                     SNAGYMELNWNQKLLQQEKSHKNESAVSTIYYRYTDEEVDHLSETKDDGTELKTKVQW
                     VGFKQQYFSSVLIADQAFESPTRINSTVINDEGAVRMMHANFTIPFSHQNNESTGMSF
                     YYGPNHYQTLNKYHIGMEKLVPLGWGIFGWVNRFAVIPVFNFLNSFNINYGIIILLLT
                     ILIKVVLLPLTYKSYISTAKMKVLQPEMSELQAKFKADPMKLQQETMALYRKAGVSPL
                     GGCLPMVLQMPILIAMFRFFPASIELRQEGFLWAKDLSTYDSILDLPFNIPFYGDHVS
                     LFTILMTVSTLIYTRMNMQMTAATNPSMKYVMYLMPIMFLGIFNNYSSGLSYYYFLAN
                     MITFGQQYLFKSFVDEKAIHAKIQENKKKPVKKSKFQERLEKMAKERGVRK"
     gene            10073..11422
                     /locus_tag="PK134_01780"
     CDS             10073..11422
                     /locus_tag="PK134_01780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRB37615.1"
                     /translation="MSLPKEFLESLPLSESDKELFVQSLQKPVPVSVRINPQKHTTHF
                     SGSSVPWCRQGYFLEQRPVFTLDPLFHAGAYYVQEASSMFLEQVIFQLSLNEKPINVL
                     DACAAPGGKTTHLLSLLHQESLVVANEVIASRTPVLVENAIRWGYSNLIITRGDAVLF
                     KRLENIFDVIVCDAPCSGEGLFRKDEAAISEWSLQNVEHCALRQQRIVNDLWDALKPG
                     GYFIYSTCTSNLQENETNVQQFINKHNAECVKLKIKDFPGVEEFQRQDAVMYRFFPHK
                     TGTEIFSLAVLRKPDLNDLNSERVVAKNLKLADIVQADFAKKHIDKGEEMFYFIHDNN
                     IRFMPICLKNALGQLLNMPVIHAGTAAFEVKGKNLLPSFELALSTALNQENFNKAEVD
                     KSTMLKLLKGETRLMDSLPIGQHLITYQNIPLMFLKKIAGRVNVNYPRSWFIRMNIE"
     gene            11494..13602
                     /locus_tag="PK134_01785"
     CDS             11494..13602
                     /locus_tag="PK134_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB37616.1"
                     /translation="MKKKATVTKQESQAKVQSGINLTQLKKYLGLLLAAIAFLLYANT
                     LNHDYTVDDGTVMQNNKIVKKGVSAIPEIFTTAYRKGFWDRNESLYRPLSVAMFAVEW
                     QIAPENPSIGHWVNVLLYVLTAWVLFRFLVNLFKDDWLFAFIATLIYVIHPIHTEVVA
                     NIKSRDEILCFLFLILSFNSFLKFLDRAKPFYALGVLFYFILSLLSKESSVTAIAIFP
                     LIAYFFRQKTIGNSIKLSWSPAIAIVAYLSLRFVVLKGISNFNEILPINNSIVNAQNY
                     IQQFATAVMIAGKYLYLLIIPYPLSFDYSYNTFPVVNFSEIKALLSLTVYVFLLIMAI
                     KGFKSRSRFSFGIFYFLLTMSIVSNVFFLIESVMAERFTYLPSLGICIALTSVLFTLF
                     KVSNVVREKIDINKIIATNRIFFGLILIVFVVYSGMTVARNAKWKNNLTLLSTDVKTC
                     PESARIRYAYGSAILIEQALKEKDYSKKMNLLDKSIEQLEKGVAILPTYSEAYYHLGL
                     AYKERENYAKAIESFEYAAKSKTFTDPQFFISWGVACGKAKRYQESIKALNHAIDLDP
                     AAKEAYLNLGVFYDEMQESDKAETSLMKAIELDSLSDGAYYNLGNVFAHRNNFVKAIQ
                     NYKKALEINPTNEDAVNNTGNSYAAMQDYENAIIYFREAIELNPSNARALNNLGVTLV
                     LTGKKDEGEQYIAKAKAIGG"
     gene            complement(13768..>14305)
                     /locus_tag="PK134_01790"
     CDS             complement(13768..>14305)
                     /locus_tag="PK134_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37617.1"
                     /translation="VQFKLSDELRKGDHIIAIETLFPHSAEFEKLRNFKTPKDSNFMG
                     LFIVASAGFFLLTSIFFLTQFFRRKESNKYTYIQLLMFPIGLIMFYYMCVLCGSVNVF
                     YFPAPYKDPANIFVSMTSYIPFLILLLILPFSVVNLRILKDNSWSFVAKGLFTFNNII
                     YFVLIGLFTYWRFYSIFN"
BASE COUNT         4602 a         2464 c         2694 g         4545 t
ORIGIN      
        1 atgactggca gaccataaat ggtgaaaatg tcatttttac ttcattttct tatttgtcgg
       61 cattagaaca aagcaagcct gccggcatgc agtttagata tgttattgtt tatgatagta
      121 agatgcctgt tgcgactttg tgttttcaat tgcttgatgt tgccaataaa gatttggatg
      181 gtgtattgaa tttaaaagac aaaggttggt tgttgcagaa tctgaataaa tcaatcaaca
      241 ggctgttatt taaatgccaa accggaaaag taaacttttt aatttgttgc ggaagttttt
      301 ttgtaagcgg tgaatatggt atcaatgtta taaatagaga tgccttgcct gcagtaattc
      361 aacttttacc ggaagtgaga cagaaaatta cagatacaat tagtaatgca tcggtgtgtg
      421 gatggatggt aaaggatttt tataacaaca ccaaggttga tcaaccattg ctggaagatg
      481 atttttttga attgccaatg gacccagaga tgatattata catcagagaa cagtggcatt
      541 cgttcgaaga atacctggca tcgctcacaa caaaatatcg tgttaaagcc aatagtgtaa
      601 taagaaagat tgaagatgtt gacgttgttg cccttaatgc agcagaagta ctaaaatatg
      661 gagatgaatt aataaggctg tatttaaata tacagcaaaa ggcaaccgtg caaatggcga
      721 gagtcaatat tttatatttt gaaaaactct taaccataat gcctgaaaaa ttttacgtaa
      781 aggttttttt taaagaaagt aaagtgatag cattttattg tgggtttcat catgccggtc
      841 atcatgaggc acattttata ggtattgatt acagtttgaa taaaacactt tctctttatc
      901 agaatatatt atatcgtttt attgaagaag ccatcagtca acgctcttct aatctttatt
      961 atgggcggac tgctatggag ataaaatcta ctaccggagc caaagcctat ccattacatg
     1021 cttattttaa tctaagtaat actgtcttga ataaaatggt gaagcctgtt gtcaggcgat
     1081 ttacaccaga ggagtggatt gcccgaaatc ctttcaggga gtaattatcc aaaaaccgtt
     1141 tgccattttt atttgaattt tcaccccaaa aattaacatg ttaacataaa acagtgcctt
     1201 gcagcattgg tggtgtgata gcttgcaagc atcttaattg aaccaccaat gattagaaca
     1261 attcaaaaca caacaaaagc agccatcata ctgattatgt tcagttatat tggcaatcca
     1321 tttcctgctt atgcaaaagg aaaaacactt aaagcaacat taaaaggcca gaatgtctct
     1381 tgttttggcg gcagcaatgg ctctatcagt cttgatattt caggcggtaa ggcgccatat
     1441 caaattacat ggaatgatgg tacgtcaggc tatgccatgt tagataagcc tgcaggcgag
     1501 tattctgttt caattacaga ttctaaaggc agtacggttt cgcagtctat tacacttact
     1561 gaaccttatg ctatgacaat ttattcgcaa gttacttccg aacagtgtta tgcccgcaat
     1621 ggtaaagcaa acttattggt aacaggcgga acacaaccat atcagtatga atggtcgaat
     1681 caaacccgtc agcaaaactt ggaggcagtt gcggcaggcg attatgaaat aaaagttaca
     1741 gataagaatg gctgtataaa gcagtcgaac attactattg acagaaacaa agttcttgag
     1801 ttaaacatag caaagtattc tcctttatgt aatgacgaag ccagtggctt aatagatgtt
     1861 gaagttaaag gtggatggtc tccatatacc tatgaatggt cgaatggtgc taaaacagaa
     1921 gatcagagtg gattaaaagc aggaaattat tctgtgatcg taaaagacaa taatggttgc
     1981 actgcaaaag cagaagttca attaaaaaat ccggaaacga taaaagttga agcaaaagtt
     2041 acagaggcgg atttgaataa aagcaatggt agcataatgt tacaggtttc aggtggaaca
     2101 ggaaattaca cctacctgtg gtcgaatatg gaaagcagtc agtcgttgaa taatgttgaa
     2161 gaaggcgttt atgctgttag ggttgatgac gcgcaccgtt gtagtgcagc agcatatttt
     2221 acagtgaatg aaaaatcgcc tatgcaggtt gaatcggttg tgaaacatga attgtgtaat
     2281 ggagggaaaa atggttcaat aaaactgaat gtaaaaggag gagttgcacc ttataaattt
     2341 caatggtcaa atggtatgaa tggagcaaca ataagtaatc ttactgccag tgattatagt
     2401 gtgctcatta ccgatgccaa taataaagtt tacagaaata cttttactgt taagcagcct
     2461 gagccaatag gaatatttgc aacagtactt gatgaaagtt atacaggaaa aaatgatggc
     2521 agcattaaat tgcaggtgat aggaggaatg cctccgttaa aatatcaatg gagcaatggt
     2581 caaaccgttg ctgatttagc aaacttatcg cagggaacat acacagtgcg aatgaaagat
     2641 ggtaatggtt gtgctatgga acaccatgta cttgtaggaa taaactatac tgatgcaatg
     2701 ttaagaactg cttttgcgaa tgaagataat gcaatgatgg aaatttatcc caatccattc
     2761 agtaacaatt ttacaatgaa attcagtaca ccggttaacg atatacaaag cattattgtt
     2821 tatacaatgg atggaaaaat tgctcagcag cttaatcctg atttaaaaca acagcaggtt
     2881 aatgaaattg ctgtcaatgc atctaaattg aaacccggaa attatttagt aaaagtaacc
     2941 acagacaaaa aaatctttac aagaatcgtt acgcgaacaa tgtagtatca atacttttca
     3001 tggttggttg tttttcacag gcgcagttgg tggttctctg cgcctgtaac cgtttttaag
     3061 ggttaggaag taattcttaa tgaagtaaaa aaatatctga aataaccata ccggcaaaaa
     3121 caatgctggc aattccattg gtagtagcaa aggcaatgtt tactttgcta agattatttt
     3181 cagaaaccaa actgtgctga taaataagca gtgcacaaaa tattgcaagc cctgaccatg
     3241 caattaaacc tacatgagcc tgataaagaa aaagagccaa aaaacctgcc gataaaaaat
     3301 gcaaaatcct tgaaacctgc aatgcctgtt tttttcccat gagtgatgga atggaatgca
     3361 atttattttc acgatcaaat tgctcatcct gcaaagcata aataatatcg aacccgctaa
     3421 cccagcaaag aacagcaaca ccaattaaaa ttggagtaat atcaaacttt cctgtaactg
     3481 ccagataagc gccaacgggt gccaatgcaa gtcctgcgcc taaaaccaag tgacaaagtg
     3541 ctgtaaatcg tttggtaaaa ctgtagccaa gcacaacaaa taatgcaacg ggcgacagaa
     3601 aaaagcaaag tttattaatt aagtatgcag tcactgtaaa aagtaaagca ttaacaatta
     3661 caaaccacaa tgcacggttt gctttaatga tgccggccgg aatttcgcgc agtgctgttc
     3721 gtggattcaa tgcatcaata cttctatcgg cccatcggtt aaatgccata gctgcattac
     3781 gtgcagttat catgcagact aaaactttta ttgccaacgg ccaaaaaaca ggcagcccgt
     3841 tttgtgaaat agccaacgaa aatccaattg cagcaaaagg aagtgcaaac acagtatggc
     3901 tgaattttac aagcgataaa tattgtgatg ttttttggag tacctgcata atttaatcaa
     3961 ttgcaaaggt aatcattcct tatggtttac attttcaaag aagctatttg ctaaattagc
     4021 ccaatgaatt ttttggcaca tctttatctt tcagacaata atgagccact tatggtaggt
     4081 aattttattg ctgatcattt aaagggaaat gactggaaaa gtttgcctga aggaataatg
     4141 tcgggtgtaa aagtacatcg ttttattgat cattttacag atacacatcc tgttgttgaa
     4201 caaagcaagg tactgttgcg tccttacttt cataaatata caccggtaat cagcgatatt
     4261 ttttacgatc attttctggc agcagaatgg gataaatatt ccaatcaacc aattgatgaa
     4321 tatgcaagat atgtttatca acttatgcat aagaattatc agtttttacc tgcacgtacc
     4381 aaagaaatgc tggtttatat ggagaacgac aactggttga aaaactacgc tacattaaac
     4441 ggaatgcata gagcgttgag tggtatgggc agacgtgcaa agtttgataa taacatgaat
     4501 caggcagcag aatttttgta tgataactat gagctgtttg aaaaacactt caaggagttt
     4561 tttccggagt tggagagtgc agtgaagaag gaaatttcta cacaaacttg attttccttg
     4621 cagcaaaaaa acacatgcgc aacaaaatga tgccatgttt aaaacgcgat atgttggtgc
     4681 tgccgtaggt gcgttcgcga tagcggatag gtaattcctg tattttaaga ttgagtttgt
     4741 aagcaccaaa aatcaaatca aaatcaccaa aaggatcaaa gtcgccaaag aactttctgt
     4801 tggtaaccaa ccggtcgtaa tcatctctga aaataacttt ggtgccgcaa agtgtatctt
     4861 taaaaggttg ctctaataac cacgtaaaag cctgactgaa gaatttgttg ccgagcatat
     4921 ttaaaaaccg catggcttct ttttccattg gataaattaa acggcagcca ttaataaaat
     4981 ctcctttatt gctggcaatg gcttcataaa atttgggcaa atcttccgga ggcatggtaa
     5041 ggtcggcatc taaaatcatc aggatgtcgc cttctgccat tgagaagcct ttgcgtacag
     5101 catcgttttt tcctttgcct tcttgtttac caactttaat tttatgtgaa gcagcatatt
     5161 tactggaaac ttcgttaatg ctgtcccagg tgtcatcggt gctgttgcct tcaataaaaa
     5221 taacttcctg ccacttgcca aatttgggaa tacgtagcaa tccattttca atattgccgc
     5281 tttcattgcg agcagggata acaatactta ctgaatattt ttcgttgaca tcttctgttg
     5341 catttgccaa gggcttagca acagagtagg tgttgataca catcaacctg aataaaggca
     5401 gctttgctat aaacttattt atcagtggag ataagagcgg aatgtttatt ggcaacaaca
     5461 tgcgcctcga ctctcgataa caatcaaagc ctgcaagaaa cagaagattg cggacatcca
     5521 tgctggtgag ccaattcaat tgtggggtct tggttttcaa gccaaggttt tctgctaagc
     5581 gtaaaaatgg ctcccataga tggttgtgat aggatacaat gattttagta cgctcatgac
     5641 aaactttttt caattgttca agaacagctt gtacgtcttc aaggtagcct gtcaggtttg
     5701 aaagaataat taaatcatat ttctcttcga gtgaaatatt ttctgcagcc atcacctgaa
     5761 aatcaatctg tggaaattgt ttttttgctt cggcaatcat tgtagggcta taatcaatgc
     5821 cggttttttg ggcagcacgg atgttgttta acaactcacc ggtgccacag ccaacttcga
     5881 gtacagaaaa tgtttcatga gaaaaataat tgcagtaaaa agttatatcg ttccaataat
     5941 aacgatgttt atgccggtca cgaattcttt tctcagcaat tttttcaaaa tggtcaaggt
     6001 atctcatcaa tgcagaaagt taatctatca ccatttcggg gatatcacct tcaacaataa
     6061 gttttccggc agtagctttt ttaatatctt caacagaaac tccgggggcg cgttcaagca
     6121 atttaaatcc ttgtggagta acatcaagaa ctgcaagttc agtaacaact ctttttatgc
     6181 atttaattcc tgtcagaggc aaagtacatt taggtaaaag ttttgattcg cctgctttgt
     6241 taacatgctg catagctaca ataatatttt ttgctgatgc tactaagtcc atagcgccac
     6301 ccattccttt aaccatctta cccggaattt tccagttggc aatatctcct tcttcgctca
     6361 cttccattgc accgagaatg gttaaatcaa ctttctgagc acgaatcatt ccaaagctca
     6421 ttgccgaatc aaaaatcact gaaccgggaa gtagcgtaac ggtttgttta cctgcattaa
     6481 ttaagtcagc atcttcttcg ccttcaaaag ggaatggccc catgccaaga atgccgtttt
     6541 ctgattgtag tgttacattt attcccttgg gaatataatt agcgactaaa gtagggatgc
     6601 ctatgccaag gtttacatac atgccgtctt taacttctcg ggctattctt tttgcgattg
     6661 cgtttttatc gagcataatt aattagataa ttagatgatg agataatttg ataattgaat
     6721 gtgcggtgct attttacaat ttattctttg aggttgaaat gattttattg atgatttttt
     6781 taattacttc taattcgatt aataaatcag tagtgtctgg ataacattcg gcatgcttac
     6841 acaaaagaag ccaatagtaa gtttcttcag tttctttagc agctatttta aatttgtgaa
     6901 taaagtctgg ttcactttcc gcattttgag cttcataagc attggctcct attgaagttg
     6961 ctgattttaa gatttgattt gcaatgatat attggtgatt tgaatttaat atttctgaaa
     7021 gatgaatgat ttttaacgaa aactcaaaag tgagtttcag gattttatta tcttcaaaag
     7081 gagaatcaaa ttttcttgac atttcaagta attatctaat tatctaatgt tcaaattatc
     7141 taatcacttc ttcctcaccg ttctctgttc tattcttttc tcatatttct cgccttggaa
     7201 aatacggtga acgtaaattc cgggtgtgtg gatttgatcg gggtcaagtg ttccggcagg
     7261 gactaattct tcaacttctg caatggtgat ttttcctgcc atagccatca ttggattaaa
     7321 atttcgcgct gttgaacggt aaacaagatt tccttctgtg tcgcctctcc aagcctttac
     7381 caatgcagca tcagaatcaa atgcatattc catcagataa tctttttcaa cgccattgta
     7441 actgaaacgt cttacttctt ttccttgtgc cacttctgtg cctacaccgg caggtgtaaa
     7501 gattgcaggc atgccatagc cggcagccat acatcttgta gccaaagttc cctgtggaat
     7561 taattcaacc tccatttctc ccgtcagcat cagtctttca aattctgcat tctcgccaac
     7621 atagcttgca atcattttct tgatctgctt ttgctgcaag agtaatccca agccaaaatc
     7681 atcaacacct gcattgtttg atatacaggt taatccttta atgccttttc gaactaatgc
     7741 tgcaatacaa ttttcgggaa taccacaaag cccaaagcca ccaaccataa gtgtcatgcc
     7801 atcacgaaaa tcttttaaag cctcgtcaac attttttaca actttattca tattatttag
     7861 agaattaatt ggatgaatta ttgtgttatt gaattattga tttaatttaa aatgtgttta
     7921 gattgattct tacaaagtaa aaagtaatat tacaatgttt caaatttaaa tttatccaat
     7981 aacattctaa tataacatca aatcattgat ttacttagat attcatttca gtcttatata
     8041 actttcaaac ctttcaactt taaaacattc aaacttatta ctttcttaca ccacgctctt
     8101 ttgccatttt ttctaatcgt tcctgaaact tcgatttttt aacaggcttc tttttgtttt
     8161 cctgaatttt tgcatgaata gctttttcat caacaaaaga tttaaagaga tattgctgtc
     8221 cgaaggtaat catatttgca agaaaataat aatagctcaa tccggaagag taattgttga
     8281 aaattccaag aaacattatc ggcatcaggt acatcacata tttcatagaa gggtttgtgg
     8341 cagcagtcat ttgcatattc atgcgtgtat agatgagtgt tgacactgtc atgagaattg
     8401 taaataaact cacatggtct ccataaaacg gaatgttgaa aggtaaatcg agaatagaat
     8461 cataggtgct taaatctttc gcccataaaa aaccttcctg tcgtaactca atagatgcag
     8521 gaaagaagcg gaacatggca atgagaatag gcatttgcaa caccataggt aagcaacccc
     8581 ctaacggact tacaccggct ttgcggtaaa gtgccattgt ttcttgttgc aacttcattg
     8641 ggtctgcttt aaactttgcc tgcaactccg acatttcagg ttgcaacact ttcatttttg
     8701 cagtactgat gtatgacttg taagttaacg gcaacaacac aactttgatg agtatggtca
     8761 gaagtaaaat gataatacca taattgatat taaaactatt caggaagttg aatactggaa
     8821 taacagcaaa gcgatttacc caaccaaaaa ttccccatcc taatggaaca agtttttcca
     8881 ttccgatgtg atatttattt agcgtttgat agtggttagg accataataa aaactcattc
     8941 ctgtgctttc attattctga tgactgaatg ggattgtaaa attggcgtgc atcattcgca
     9001 cagcaccttc atcattaatt actgtactat taattcttgt tgggctttca aaagcctggt
     9061 cagcaattaa tacacttgaa aaatattgtt gcttaaatcc tacccattga accttggttt
     9121 ttaattctgt tccatcatcc ttagtttcgc tcaaatggtc aacttcttca tcagtatagc
     9181 gatagtaaat tgttgaaaca gcactttcat ttttatggct tttttcctgt tgcagtaatt
     9241 tctgattcca atttaattcc atataacctg cattggaagc taccaatgac cccatattca
     9301 cgaagttaat tttaaagcct acacgataat catcaccttt caatgaataa acatattcaa
     9361 tgtaacggcc ttcaccggca tttaacttta tagaaacaga ctgcgagtct tttcctgtca
     9421 cggtgattcc ttttgcaggt aaatcaaaat acaaatctcc ggtagaaatc attttgtttt
     9481 ctgaaaagaa gctcaggctg aattgagagc tatctccatt gttcaacacc aaaggttttc
     9541 cctcaccggt tttatatttt tttaattcaa cataatcaat ctttccgccc ttgctgttaa
     9601 gctttaattt cagcaaatca ctttccaaag taatactttt gctttcacct tgcgcagcta
     9661 atgcaaatgc tccaaaatta tctgttagtt ttgtttgtga tagtgagtcg ttgtttagcg
     9721 tgtcggcaac tgctacggtg tcagctttca gtgcagctac gggtttggtg ataagtttca
     9781 aactgtcctg atgcttcttt tcaaccaatg caatagagtc ttgctttcgt tttgctgctt
     9841 cctgctcttc ttttgaaggc tgattgaata ttgaaaagcc aatcaacaca gccataatta
     9901 aaactactcc taaaatcgaa tttctgtcca tatttttaaa aaacacggca aagataataa
     9961 atgaagcata ggaggaggct ctaagaaatt cagaatatga ctgtaagtca gagtgatata
    10021 aaactgtgca gtttcatgta tttttacaac cacaacctgt tatatcatat tcttgtcgtt
    10081 gccaaaagaa tttctggagt cgctgcctct ttctgaatca gataaagagt tatttgtgca
    10141 gtcattgcaa aaacctgtgc cggtgtcagt gaggataaat ccccaaaagc atactacaca
    10201 tttttcagga agttcggttc cttggtgcag acaaggatat tttttggagc agcgacctgt
    10261 ttttactctc gacccattgt ttcatgcagg ggcatactat gttcaagagg cttcaagcat
    10321 gtttttggag caggtaattt ttcaacttag cctcaatgaa aaaccaatta atgttcttga
    10381 tgcctgtgct gcaccgggag gaaagacgac ccatttatta tcattacttc atcaggagtc
    10441 gcttgttgta gcaaatgaag ttattgcttc acgtacacct gttcttgtag aaaatgctat
    10501 tcggtggggc tattctaatt tgattatcac acgtggtgat gcggtacttt ttaaacgttt
    10561 ggaaaatatt tttgatgtta ttgtttgcga tgctccttgc tcgggagaag ggctgttcag
    10621 gaaagatgaa gccgctataa gtgaatggag tctgcaaaat gttgaacatt gtgctttacg
    10681 ccaacaacgt attgtaaatg atttatggga tgcattaaag cccggaggtt attttatata
    10741 ttcaacctgc acatcaaact tgcaggagaa tgaaaccaat gttcagcaat ttataaataa
    10801 gcataatgct gaatgtgtaa aactcaaaat taaagatttt ccgggagttg aagaatttca
    10861 aagacaagat gctgttatgt atcgtttctt tccacataaa acaggaacag aaatattttc
    10921 gttagcagtg ttaagaaaac ctgatttgaa tgatctaaat tctgaaagag ttgttgctaa
    10981 gaatctgaag ttggccgata tagtacaggc agactttgcg aaaaaacata ttgacaaggg
    11041 agaggagatg ttctatttta tacatgataa caatataaga tttatgccta tatgtttaaa
    11101 aaatgcatta ggtcagctgt taaatatgcc ggtaatacat gcaggtacag cagcatttga
    11161 agtaaaaggt aaaaatttgt tgccgtcatt tgaacttgcc ttgtcaacag cgttaaatca
    11221 ggaaaatttc aataaagcag aagttgataa atctaccatg ttaaaactac tcaaaggtga
    11281 aacacgtttg atggattcgt tgccaatagg ccagcattta ataacatatc agaacatacc
    11341 attgatgttt ttaaagaaaa tagcagggcg agttaatgtt aactacccac gttcatggtt
    11401 tatcagaatg aacattgaat aggttgaaaa atgctttgta agtagtgctg tattttttgt
    11461 aatttcactt tactttgttt caaacaacat aggatgaaaa aaaaggcaac ggttacaaaa
    11521 caggaaagtc aggcaaaagt acagtcagga attaatttaa cacagttaaa gaaatatctt
    11581 ggattgttgc ttgctgcaat agcattttta ctttatgcca atacactcaa ccatgattat
    11641 actgttgatg atggcacagt gatgcaaaac aataaaattg taaagaaggg agtaagtgct
    11701 attcccgaaa tttttacaac tgcatatcgc aaaggctttt gggacaggaa tgaaagtttg
    11761 tatcgcccgt tatctgtagc aatgtttgct gttgaatggc agatagctcc agaaaatcct
    11821 tctattggtc attgggtaaa tgtattgctc tatgtgctca cagcatgggt attgtttcgt
    11881 ttcttggtta atctttttaa agatgactgg ctgtttgctt tcatcgcaac actcatttat
    11941 gtcattcatc ccattcacac agaggttgta gctaatatta aaagccgaga cgaaattcta
    12001 tgcttcttat ttctgattct ttcattcaac agttttttga agtttttgga tagggcaaaa
    12061 ccgttttatg ctttaggagt tttgttttat tttattttat cactcctgtc gaaagaaagt
    12121 tcagtcactg caatagcgat atttccattg attgcatatt ttttcagaca gaagacaatc
    12181 ggtaacagta ttaaattgtc atggtcaccg gcaattgcta ttgtagccta tctgtcattg
    12241 cgctttgttg ttcttaaagg aatctctaac tttaatgaaa tattacccat caacaattca
    12301 attgtcaatg cgcaaaacta tatacagcaa tttgcaactg ctgttatgat tgccggtaag
    12361 tatttgtatt tactcatcat tccttatcca ctgtcatttg attattctta caacactttt
    12421 cctgttgtaa atttttctga aataaaggca ttgttgtcat tgacagttta tgtctttttg
    12481 ctgattatgg ctattaaagg atttaagtcg cgcagcagat tttcttttgg tattttttat
    12541 tttctgctta ccatgagtat tgtaagcaat gtatttttct tgatagaatc tgtaatggca
    12601 gagcgtttta cctacctgcc ttcattaggc atttgcattg cattgacctc tgttcttttt
    12661 actttattta aagtcagtaa tgttgtcaga gagaaaattg atatcaataa gattatagca
    12721 acaaatagaa tatttttcgg acttatatta attgtttttg ttgtttatag cggaatgaca
    12781 gttgcaagaa atgcaaaatg gaaaaacaat ctgaccttgc tgtcaactga tgtaaagacg
    12841 tgccccgaaa gtgctcggat aagatatgcc tacggcagtg ccattcttat tgaacaggct
    12901 ttgaaagaaa aggattattc aaaaaagatg aatcttctgg ataaaagtat tgagcaattg
    12961 gaaaaaggtg tagctatttt gccaacttat tcagaggctt attatcatct tggtttggca
    13021 tataaggagc gagaaaatta tgctaaggcc attgagagct ttgagtatgc agctaagagt
    13081 aaaacattta ccgacccgca gttttttatt tcatggggtg ttgcatgtgg aaaagcaaaa
    13141 cgttatcagg agagtattaa agctttgaac catgccattg atcttgaccc tgcagcaaaa
    13201 gaagcatatc ttaatttggg tgtgttttat gatgagatgc aagagtctga taaagccgaa
    13261 acctctttaa tgaaggcaat tgaattagat tctctttctg acggagccta ttataatttg
    13321 ggaaatgttt ttgctcacag aaataatttc gttaaagcaa ttcagaatta taaaaaggct
    13381 ttggagatta accccacaaa tgaggatgca gtaaataata ctggaaacag ttatgctgcc
    13441 atgcaggact atgagaatgc aataatttat ttcagagagg caattgaact gaatccttcg
    13501 aatgcaagag cattaaataa tttgggtgta accttagtgc tgacaggaaa aaaagatgag
    13561 ggtgaacagt atattgcgaa ggctaaggcg atagggggat aatagatgat ttgatgattt
    13621 gatgatttga tcatttgaaa atttgaagtt agagatttag aggataaata aattaacgaa
    13681 ttttaaaggt gcgaatgagg tgaaatactc atgaagagga atttacatta gatacttcat
    13741 cataagtcgt atttgcatcg tttctgttta attaaaaatg ctgtaaaatc tccaatatgt
    13801 aaataatcca atgagaacaa agtaaatgat attgttaaat gtaaaaaggc ctttggccac
    13861 aaaactccaa ctattatcct ttaaaattct taaattaaca acagaaaacg gtaatataag
    13921 aagtaatatt agaaatggta tgtaggaagt catgctaaca aaaatattag ccgggtcttt
    13981 atatggtgca ggaaagtaaa atacattaac actgccacat aatacgcaca tataataaaa
    14041 catgattaag ccaattggaa acattaagag ctgaatgtaa gtgtacttat tactttcttt
    14101 tcttctgaaa aattgagtaa gaaaaaagat agaagtcagt aaaaaaaatc cggcagaagc
    14161 aacaataaat aatcccataa aattactgtc tttgggggtt ttgaaattac gtaatttttc
    14221 aaattcagca ctatgcggga atagtgtttc tattgcaata atgtggtcgc cttttcttaa
    14281 ttcatcactt aacttgaatt gaaca
//
LOCUS       DAORQT010000023        13639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_467_length_13639_cov_10.428004, whole genome shotgun sequence.
ACCESSION   DAORQT010000023 DAORQT010000000
VERSION     DAORQT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_467_length_13639_cov_10.428004"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PK134_01795"
     CDS             <1..1022
                     /locus_tag="PK134_01795"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB37618.1"
                     /translation="TVNVSVTTTKENLSGILNLIGEIIKQPAFAQAEFDKLKEEQISD
                     LESSRSEPQAIAFRVYSKTLNPYPKDDFRYEMSFDEEKEALNKLTLADVKNFYERFYT
                     TSNATAAIVGDFDEPSLIISLNNLLDKWGSPVLYERAKEPYFDVTPVNEKINTPDKAN
                     ALMVAGMNLPLKDDNADYPALMVGNYMLGGGFLNSRLATRIRQKEGISYGVGSQLQAS
                     SLDESGGFLSYAIYNPDNSDKLIAAWKEEVQKMLNEGFTNAELTDAVKGLIQARSVSR
                     SQDRELCSKLNSYTYLGRSIKWDEEFENKIKALKVEDVNAAMKKWINPNKVSFVQAGD
                     FERKK"
     gene            1251..6365
                     /locus_tag="PK134_01800"
     CDS             1251..6365
                     /locus_tag="PK134_01800"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB37619.1"
                     /translation="MKKIFGFIVVFLTLSINNLLYGQQPSSCTNLDFELGNFVGWTGK
                     TGSCCPISMFSNGIVTGQHTIMSGTGFDPHSNNQIPVVAPGGTYSARLGNDQAGSQAE
                     QLSYSFQVTPQTELFIYRYAVVLEDPVHDPTEQPRFEIRVFDQAGNPVGCGTYNVVSG
                     AGIPGFQTWLDPNIGSDIHFKTWTTVGIELSSYMGQTVTIEFSTGDCSLGGHFGYAYV
                     DAYCSSMTVTSDYCPGSSTALLIAPPGFASYLWSNGATTQTITINNPVIGATFSVQMT
                     SVTGCVVTLNQVIAVTQLFANFNFNPGCESGTQFFDSSYVLSGSPVNQWLWDFGDGTT
                     STMQNPWHQFPASGNYNVTLTATNAGGCTKSITKSISILPSPVSDFNFTPACPGQAIS
                     FNQNASFPSGTITSYEWLFGDGLPSSAVPDPTHVYSGMGPYDVSLIVTGNNGCVDTIV
                     KPVAPMPDPVAGFTFSVQCMNNTVTFTDVSQLTGGTITAWQWDFGDGSPLDNTANPVH
                     VYNGGGNYAVQLIITGSNGCTDTITKGVAMASLPTAAFTNSGACESQFTAFNDASSLT
                     GGSISSWLWYFGDGTNSTLQNPFHQYNGTGSFNISLVVLGDNGCRDSMARSITVKPSP
                     VASFTSLPVCPGQQAQFNSTAVAPAGETISSWIWNFGDGSAQTAGQNVNHTFTGAGTY
                     AVMSIVGASNGCIDSVVNNISTSITPVAKFSAPSGCALVPLSFSSTSTISSGTITGWD
                     WDFGDGTAHGTTAATTHAYAAPGSYNTYLTVTSNNGCTHTVLHEVSVSGKPVPSFTNS
                     SSCVNDSINLNSVSTSANGSINYLKWDFGDGTFLTGTDSVFHVYADTGFVQIGLIVGN
                     TLGCSDTVYRQIYIKPLPVAGFRWDEMCQSAPGSFYDTSVLINGNISGWLWDFGSGTT
                     NTANPQFAFSNYGVQPVTLTVTGSNNCKATISRNITVRPKPLAQFVSSSACVNAAETF
                     QNTSLISSGNIVSWQWNFGDGHSSISQHGQNTFTTDGYYQVQLFAISDFACADTALDT
                     IRVFPLPVANFFAPHVCLGNATVFNDLSQPISDSLITWSWNFGDGSTSTMPTPSHIFA
                     QDTIYPVSLTVQNEGFCSKTITKNVIIRPLPEVGFTFANPCVGSPVQFQDTSALSGYN
                     INWWQWNFGDSATASIASPVHTYSLSGNIFVKLIVRATNGCSDSVSVPVLVNHLPDAV
                     FSVSDICEQAVLTPNNQSALSGGNITNTLWQWGDNTSDSIFSPTHFYQSDGNFNINLE
                     VTGSNGCKNDTTIAIVIHDKPIASFTHSNACQLAPVAFYDQSSTVNDSLQIWQWQFGD
                     GDQSNLQSPVHDFGAYGVYYTELIVTNSFGCRDTVADSVTVAPKPQAAFSSVNVCKDQ
                     PMIFNNSSSVVAGSIVSNQWSFGDGSSAAVANPTYTYLSTGDYEITLVVTTANQCVDS
                     IKHIYRVYQLPTAGFIADTVEGCQPLLVLFSDSSVANEGLITMWNWNFDNGASDTLTN
                     PAAIVYDQAGYFSPVLYIETNLGCRDTFLRQDYIHVFAKPEAAFIAEPLSNTILHPLI
                     EIKDQSWNTVSWFYDFGDTTNTTEQNPSHWYKQPEHYTIIQYVESADGCKDTALISIE
                     IKNDFTFYMPNSFTPNQDGFNETWNAFGIGITEFNVQVFNRWGQLLFTSNDLSKGWDG
                     TYSGNKVQQGVYVYKATVRDLFNKQHFYVGNVNVIR"
     gene            6378..6668
                     /locus_tag="PK134_01805"
     CDS             6378..6668
                     /locus_tag="PK134_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRB37620.1"
                     /translation="MNFRIKENALIAWIAARFLGYKTMAIVIGKTIYLHGASVKTFQS
                     DSKWMKHELKHIEQFKRYGFLRFIFLYIIESIRHGYINNRFEIECREAENQD"
     gene            complement(6693..7571)
                     /locus_tag="PK134_01810"
     CDS             complement(6693..7571)
                     /locus_tag="PK134_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRB37621.1"
                     /translation="MEKRSVEIVVSPREPHFVGDGFRVHNFIPSGYRLDMQRMNPFIM
                     LDYNSKFHFDPSDTPKGVGVHPHRGFETVTIAYKGKVEHHDSSGGGGVIGEGDVQWMT
                     AASGVLHKEFHEKEWSKTGGEFQMVQLWVNLPAKDKMSKPKYQALENSKINRFKSDDK
                     KTEIEIIAGEYKGVKGSASTFTPLHLYNVKMAKDAKTEMNLPKNFNTALLVIEGSITI
                     NGTEKVDTNHFALMDNDGESFTIDSNENAVYLVLSGEPINEPIAAHGPFVMNTRQELV
                     QAFEDFNQGRFGTLQE"
     gene            7756..8148
                     /locus_tag="PK134_01815"
     CDS             7756..8148
                     /locus_tag="PK134_01815"
                     /inference="COORDINATES: protein motif:HMM:NF022264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HRB37622.1"
                     /translation="MKQRISVVMGLLIMVTMVSLSFKPDNWRILGTKTVDYQLDKDVL
                     DVQLRDGVFQKLKFVVRGGALNMHKVIVHFENGGEQEIDMRHSFDKNSQSRVIDLKGN
                     KRIIEKIVFWYDTKNASNRKAKVTVLGR"
     gene            complement(8161..8523)
                     /locus_tag="PK134_01820"
     CDS             complement(8161..8523)
                     /locus_tag="PK134_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRB37623.1"
                     /translation="MNSTEKNIERVAKMSFASIYPLYISKIQKKNRTSEELNQVITWL
                     TGYSVKELQKHLDNKTTFQEFFQQAKINPNASKITGTICGYKIEEIENPFMKKVRYLD
                     KLVDELAKGHPLTKILRS"
     gene            complement(8529..8921)
                     /locus_tag="PK134_01825"
     CDS             complement(8529..8921)
                     /locus_tag="PK134_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRB37624.1"
                     /translation="MEKSIQTYNNSQSQEDKKICDLLADSIDTVLKTAECKIWHAHPV
                     WFIDENPIVGYSKQKLGWRLMFWSGIDFEEEGLNVHGKKFKDASVFYTSIEQINIKDL
                     KRWLKKSITIQWDYKNLIKRKGKLVRLK"
     gene            complement(9027..9536)
                     /locus_tag="PK134_01830"
     CDS             complement(9027..9536)
                     /locus_tag="PK134_01830"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRB37625.1"
                     /translation="MKAKTAKESLTIQTEIVLPNDTNVLGNLMGGRLLHWMDVCSAVA
                     AHRHCGRVVVTASVNNVSFNQPIRLGDIVTIQAKVSRSFTSSMEVFMEVWVERNATGD
                     KIKCNEAIYTFVAVDQQGSPIAVPELIAESEEEKKRFDGALRRKQLALILAGKMKPAD
                     ATELKALFF"
     gene            9650..10375
                     /locus_tag="PK134_01835"
     CDS             9650..10375
                     /locus_tag="PK134_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRB37626.1"
                     /translation="MQTKLIALTAIIGMMLNGKMAHSQTPVNMSETRKIEVTGTAEMK
                     VIPDEIFVVITIQEYTENKKKTDINEVRKNFLAACTKAGIAKENISIQGMNGYNNSGW
                     YWQRKKKTPDFVASSSYIIKFNSGEQIERLMSQLDDKGTQNMYISSTSHSKMDEFKKQ
                     VKTEALQAAKAKATYLCEGIGEKVGKVLLIKEVEGTNYAEPMMMKSRMMEASMADSNF
                     EPSGLEFQKITIQMEILAQFAIQ"
     gene            10383..>10830
                     /locus_tag="PK134_01840"
     CDS             10383..>10830
                     /locus_tag="PK134_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37627.1"
                     /translation="MQNSKGRKRGNSDKPQAGRYKSEGRPSRGKKFGSSTDKSKEEFV
                     KSGNSYSKNKFSGKRRFDADESGFERKRKNFGSDDNAGFEKRERPSRFKKEGGFEREK
                     RSFKSSDSPRPYRKRDEGFDGEERPSRFKKEGGFEREKRSFKSSDSP"
     assembly_gap    10831..10930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10931..12003
                     /locus_tag="PK134_01845"
     CDS             <10931..12003
                     /locus_tag="PK134_01845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB37628.1"
                     /translation="PYRKRDEGFEGDEKPRFKKRSDFGGSGKFDKSKSFKRRDDNVEG
                     EDKPRRFSKSFDSDTREKRSYDRKERSSGRFDKPYRKKNDNRKYSKKEKESSEQLSDG
                     LVRLNRYIANSGICSRREADELITAGLVSVNGEIVTTMGTKVSQDDVIKYNGETIRNE
                     KPMYLLLNKPKDYITTMDDPQDRRTVMHLIAGACKERVYPVGRLDRNTTGVLLFTNDG
                     DLAKKLTHPSYEVQKIYHVELDRNLKSSDKEKIKEGVMLDDGMATVDDIQYDGGFNDK
                     KQIGVEIHSGKNRIVRRIFESMDYNVIKLDRVVFAGLTKKDIARGKWRFLSEMEVNAL
                     KMATSKVKPKDKEPRKRISRSK"
     gene            12020..13192
                     /locus_tag="PK134_01850"
     CDS             12020..13192
                     /locus_tag="PK134_01850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB37629.1"
                     /translation="MSSTALHGLIIASGIWRAVLMTPGGELPFNFETREVNSRSEIFI
                     KNGNEEIEVNEISYANDSLIIQLPVYDSEFRVRVSGDSMIGFWYNNSRKNYAPTPFKA
                     FLGEKYRFFNNTKSEIKLPEKWEWKFSPGTKDSSQAIALFKQNGTDVRGTLLTESGDY
                     RFLDGCLNNDSLFLSYFDGAFAYLFKGSIKNNMMQGVFYSGHHWQQPWQAVVNESAEL
                     PDPFKLTFLKNDTASFSFSALRLDSTVFSYPNKDLKGRVVVVEMMGTWCPNCMDETAF
                     LSDYYSRNKSQDLTIIGLAFEKTSDFRKSVGNVKRVKEKYHVQYETLIAGNREGAASK
                     MSMLSKINGYPTTIFIDRKGKVRKIYTGFNGPATGAYHERTKDDFIRIITTLLSEK"
BASE COUNT         4315 a         2385 c         2654 g         4185 t
ORIGIN      
        1 aaactgttaa tgtatcggta acaactacta aagaaaacct cagcggtatt ctaaatctga
       61 taggtgaaat cattaaacaa cctgcatttg cacaggcaga atttgataag ttaaaagagg
      121 aacaaatttc tgatcttgaa agctcacgca gcgaaccaca ggcaattgca ttcagggtat
      181 atagtaaaac actgaatcct tatccaaaag acgatttccg ttatgaaatg agttttgacg
      241 aagaaaaaga agcattgaat aagcttacat tggctgatgt aaagaatttt tatgagaggt
      301 tttataccac ttcaaatgct acagcagcaa ttgttggtga ttttgatgag ccatcattaa
      361 ttatttctct gaacaattta cttgataaat ggggttcacc ggttttatat gagcgtgcta
      421 aagaaccata ttttgatgtt actcctgtaa acgagaaaat caataccccc gacaaagcga
      481 atgctttgat ggtggcagga atgaacttac cattgaaaga tgacaatgca gattatccgg
      541 cactgatggt tggaaattat atgttgggcg gaggtttctt gaattcacgc cttgcaacac
      601 gcatcaggca gaaagaaggc atcagctatg gtgtaggctc acaattgcag gcaagttctt
      661 tagatgagtc aggtggattt ttatcttatg caatttacaa tcccgataac tcagataaac
      721 tgattgcagc atggaaagaa gaggttcaga aaatgttgaa cgaaggattt accaatgcag
      781 aactaacaga tgctgtgaaa ggattaatac aggcacgttc agtaagccgc tctcaggaca
      841 gagagttgtg cagcaaactt aacagctata cctatctcgg tcgctccata aaatgggatg
      901 aggaatttga aaataaaatc aaagccttga aagttgaaga tgtaaatgca gcaatgaaaa
      961 aatggattaa tccgaataaa gtttcttttg tgcaggcagg agattttgag aggaagaagt
     1021 aagcgttatt tgataaagta tctataaaaa tatttacaaa gaatcctgct taatgcagga
     1081 ttctttgttt gtataaacca gaacacttag aagtagtagt ttgaaaatat ttgaacagaa
     1141 acttttgctt tcagagctaa aaaatttaaa ataaaagttc ctttttttaa aaaaatctgt
     1201 tacttttacc caattaaaga accggccaac taaacaagat atatctggtc atgaaaaaga
     1261 tatttggttt tattgttgtt tttctaacgt tgtctataaa caatttgctt tatgggcagc
     1321 agccaagtag ttgtaccaat cttgattttg aactcggaaa cttcgtgggc tggactggaa
     1381 aaacaggttc atgctgccct atcagtatgt tttcaaacgg tattgttacc ggacagcata
     1441 ctattatgtc aggtacagga tttgatcctc actctaacaa tcagattcct gttgttgcgc
     1501 ccggtggaac ttactctgca cgtttaggta atgatcaggc aggctcacaa gcagagcaac
     1561 tttcttattc ctttcaggtt actccacaaa cagaattatt tatttatcgc tacgcagttg
     1621 ttttagaaga ccctgtgcac gaccctacgg aacaaccacg ttttgagatt cgtgtatttg
     1681 atcaggcagg taatcctgtt ggttgtggaa cttacaatgt ggtttcaggt gccggcattc
     1741 ccggttttca aacctggctc gatcccaata taggcagcga tattcatttt aagacatgga
     1801 caacagtagg tattgaactt tcatcctaca tgggtcaaac tgttaccatt gaattttcta
     1861 caggtgattg ctctttaggc ggacactttg gttatgccta tgttgatgcc tattgcagca
     1921 gcatgactgt tacaagcgat tactgccccg gtagctcaac agcactactg atagcacctc
     1981 ccggatttgc atcctacctg tggagtaatg gtgcaacaac acaaacaatc actatcaaca
     2041 accccgtcat tggtgctacc ttttctgttc agatgacatc tgtgacagga tgtgtggtaa
     2101 cattgaatca ggttattgcc gttacacaac tgtttgctaa ctttaacttt aatccgggct
     2161 gcgaaagtgg aactcagttt ttcgattcat cttatgttct ttcaggttca ccggtgaatc
     2221 aatggttgtg ggattttggt gacggcacca cttcgacaat gcagaatccg tggcatcaat
     2281 ttccggcatc aggaaattat aatgtaacac ttacggcaac caatgcagga ggttgtacaa
     2341 aatcaattac taaatcaata agtattttgc catcgcctgt ttcagacttt aattttacac
     2401 ctgcatgtcc aggtcaagct atatctttta atcagaatgc aagttttcca tcaggaacaa
     2461 ttacaagtta tgaatggttg tttggcgatg ggcttccttc atcagcagta cctgacccaa
     2521 cacatgttta cagtggtatg ggaccttatg atgtttcact tattgttaca ggaaataatg
     2581 gctgtgtaga tactattgta aaacctgtgg caccaatgcc tgatccagta gcaggattta
     2641 cattcagtgt gcagtgtatg aataataccg ttacgtttac tgatgtttct caactgaccg
     2701 gtggcaccat aactgcatgg caatgggatt ttggagatgg ctcaccatta gataatactg
     2761 caaatcctgt acatgtttac aacggtggcg gcaattacgc agttcaactg attattacag
     2821 gcagcaatgg ttgcactgat accattacca aaggtgtggc tatggcaagt ttgccaacag
     2881 cagcttttac taacagtggc gcatgtgaat cgcagtttac agcatttaat gatgcatcaa
     2941 gtcttacagg tggcagtatt tcatcctggc tgtggtactt tggtgatgga acaaattcaa
     3001 ctttgcaaaa tccatttcac caatataacg gtacaggaag ttttaatata tcattggttg
     3061 ttttaggcga taatggttgc cgcgattcta tggcacgtag tattacagtt aaaccttctc
     3121 ctgtagcatc atttacctca ttgcccgttt gtcccggaca gcaggctcag tttaacagta
     3181 ctgctgttgc acctgccggt gaaaccataa gttcgtggat ttggaatttt ggtgatggca
     3241 gtgcgcaaac agcgggtcag aatgttaatc acactttcac cggtgccggc acttacgcag
     3301 tgatgagtat tgttggtgct tcaaatggtt gcattgatag tgttgtaaat aatatttcaa
     3361 cttcaataac acctgtagct aaattttctg caccatcagg atgtgcctta gtgccgctat
     3421 cattttccag tacatcaact atatcttcag gtaccataac aggatgggat tgggattttg
     3481 gcgatggaac tgcacacggc actacagcag caacaacaca tgcctatgca gcaccgggaa
     3541 gttacaatac atatttaact gttaccagca acaatggttg tacacataca gtattgcatg
     3601 aagttagtgt tagcggcaag cctgtgccat catttacaaa ctcttcaagt tgtgttaatg
     3661 acagcatcaa tctgaacagt gtttcaacat ctgcaaatgg aagtatcaat tacttgaaat
     3721 gggattttgg cgatggtact ttcctcacag gaacagatag tgtttttcat gtttatgcag
     3781 atacaggttt tgtgcagatt ggattaattg taggtaatac acttggttgc agcgacactg
     3841 tttacagaca gatttatatt aagcccttgc ctgttgccgg attccgatgg gatgaaatgt
     3901 gtcagtcagc accgggaagt ttttatgata cttcagtatt gataaatgga aatatttcag
     3961 gatggctttg ggattttgga agtggcacta ccaatactgc caacccgcag tttgcatttt
     4021 caaattacgg agttcaaccg gttacattaa cagtaacggg tagcaataac tgtaaagcaa
     4081 caatctctcg aaatattacc gtgcgaccaa agccattagc acaatttgtt tcatcaagtg
     4141 cctgtgtcaa tgcagctgaa acttttcaga atacatcatt aatatcctca ggaaatattg
     4201 taagctggca atggaatttt ggcgatggac acagttctat atctcagcat ggacaaaaca
     4261 cttttacaac tgacggatat tatcaggttc aattatttgc aatatcagac tttgcatgtg
     4321 ctgacacagc acttgacact attcgcgtgt ttcctttacc tgttgccaac tttttcgcac
     4381 cgcatgtatg tttgggtaat gccacagttt ttaatgactt gtcacaacca atttccgata
     4441 gtttaattac atggtcgtgg aattttggtg atggctctac ttcgacaatg ccaacaccgt
     4501 cacatatttt tgcacaggat acaatttacc cggtttcact gacagtacag aatgaaggct
     4561 tttgcagtaa gacaattaca aagaatgtaa tcatcaggcc tttgcctgaa gttggcttta
     4621 catttgccaa tccatgtgtt ggcagccctg tacagtttca ggatacatcg gctttatcag
     4681 gatataatat caattggtgg caatggaatt ttggtgacag tgctactgcg tcaatagcat
     4741 caccggtaca tacatattca ctgtctggaa atatttttgt gaaactgatt gttcgggcta
     4801 caaatggttg ttcagattct gtttctgttc ctgttttagt taatcattta cctgatgcag
     4861 tcttttctgt aagtgatatt tgtgagcaag ctgtattaac gccaaacaat caatcagcat
     4921 tatcgggagg aaacattaca aacactttat ggcaatgggg tgataataca agtgattcaa
     4981 ttttttcacc aacacacttt tatcaaagtg atggcaactt taatataaac cttgaggtta
     5041 caggaagtaa tggttgtaag aatgatacga ccattgccat agtaattcat gataaaccta
     5101 tagcctcatt tacacatagc aatgcatgtc agttagctcc tgttgcattc tatgatcagt
     5161 cttctactgt aaatgactca ttgcaaattt ggcagtggca gtttggtgat ggtgatcaat
     5221 caaatctaca aagtcctgtg catgattttg gtgcttatgg tgtttattat acagagctca
     5281 ttgtaactaa tagtttcggc tgtcgtgaca cagttgccga ttccgttact gttgcaccta
     5341 aaccgcaggc agcattttca tcagtaaatg tttgtaaaga tcaaccaatg atttttaata
     5401 attcaagtag tgttgttgca gggagtattg tttccaatca gtggagtttt ggtgatggct
     5461 cttctgctgc tgtagctaat cctacctata catatttaag taccggtgat tatgaaatta
     5521 ctttagtagt tacaacagcc aatcaatgtg ttgatagtat aaaacatatt tatcgtgttt
     5581 atcagttgcc cacagcggga tttattgcag atactgttga aggatgtcag ccccttttgg
     5641 ttttattttc tgatagctca gttgccaatg aagggttaat taccatgtgg aattggaact
     5701 ttgataatgg tgcatcagat actttaacaa atcctgcagc aattgtttat gatcaggcag
     5761 gatatttcag tcctgtattg tatattgaaa caaatttagg atgtcgcgat acctttttac
     5821 gacaggatta cattcatgtt tttgcaaaac ctgaagctgc atttattgca gagccattaa
     5881 gcaatacaat tttacatcca ctgattgaga ttaaagatca gtcatggaat acagtatcat
     5941 ggttctatga ttttggtgat accacaaata caacagagca aaatccttca cattggtata
     6001 agcaacctga acactatacc attattcaat atgttgaaag tgcagatggt tgcaaggaca
     6061 cagcattaat cagcatagaa attaaaaatg actttacttt ttacatgcct aactctttca
     6121 ctccaaacca agatggcttt aatgaaacat ggaatgcttt tggtattggc attacagaat
     6181 ttaatgtgca ggtttttaat cgctggggcc agttactatt tacatcaaat gatttgagta
     6241 aaggatggga tggaacctat tcaggaaaca aagtacaaca aggtgtttac gtatataaag
     6301 caaccgttcg cgatttattc aataagcaac atttttatgt tggaaatgta aacgtaatca
     6361 gatagtaacc ccagtaaatg aatttccgga ttaaagagaa tgcactgata gcatggattg
     6421 cagcccgttt tttaggctat aaaacaatgg caatagtcat tggaaaaaca atttacctgc
     6481 atggtgcatc agttaaaact tttcagagtg attccaaatg gatgaagcat gaactcaagc
     6541 acattgaaca atttaaacgc tacggttttt tacggtttat ttttctctat ataattgaat
     6601 caattcgtca tggatatatc aataaccgct ttgaaattga atgtagagaa gccgagaatc
     6661 aggattgaat tttattctga aatagaaaaa aattattctt gcaaagtgcc aaatcgtccc
     6721 tgattaaaat cttcaaaagc ctgcactaat tcctgtcttg tattcattac aaaagggcca
     6781 tgtgctgcta ttggttcatt aatcggttcg ccactaagca ccagataaac tgcattttcg
     6841 tttgaatcaa ttgtaaaact ttcaccatca ttatccataa gagcaaaatg attggtatct
     6901 actttttcgg ttccatttat agtaatgctt ccttcaataa ccaataacgc tgtgttgaaa
     6961 tttttaggca gattcatttc tgttttagca tcttttgcca tcttaacatt gtaaagatgc
     7021 aatggtgtaa aagtagaggc acttccttta actcctttat attctcctgc aataatttca
     7081 atttctgttt ttttatcatc agatttaaaa cgattgattt ttgaattttc taatgcctga
     7141 tattttggct tactcatctt gtcttttgca ggaaggttca cccacaactg taccatctga
     7201 aattcgccac ctgttttgct ccattctttc tcatgaaatt ctttatgcaa aacaccactg
     7261 gctgcagtca tccattgaac atctccttca ccaattactc cgccaccacc gctgctgtca
     7321 tgatgttcta ccttaccttt ataggcaatg gtaactgttt caaatccacg atgcggatgt
     7381 actccaacac ccttaggagt atcactgggg tcaaagtgaa atttagaatt ataatctaac
     7441 atgatgaatg gattcattct ttgcatgtca agacgataac cacttggtat gaaattatgt
     7501 actctgaagc catcacctac aaaatgtggt tcacgtggac taacaactat ttctacagat
     7561 cttttttcca tgataatgta tatttatagt gcaaaattat tatcagccct tcctttttac
     7621 aataacgtag attaagtatt tgttttaagt cagtttactt acaaagagac agaaaaaatt
     7681 cataaagcag attaaaccat tatttcataa aatagtctat tagtttgaaa aagcaacaat
     7741 attaatttta aaaatatgaa acaaagaatt tctgtagtaa tgggcttatt aattatggta
     7801 actatggtaa gtttatcttt taagcctgac aattggcgca tcttaggcac aaaaacagtg
     7861 gactatcagt tagacaaaga tgtgttagat gtgcagttgc gtgacggtgt ttttcagaaa
     7921 ctgaaatttg ttgtcagagg aggtgctttg aatatgcata aagttattgt tcacttcgag
     7981 aatggaggcg aacaggaaat tgatatgcgt catagttttg ataagaacag tcaaagcaga
     8041 gttattgacc tgaaaggaaa caaacgcatc attgaaaaaa ttgttttttg gtacgatact
     8101 aaaaatgctt caaaccgaaa agcaaaagtg actgtgttag gacgttgatg aaataatatt
     8161 ttaagaccgt aaaatcttag ttagtgggtg accttttgca agctcgtcca ctaacttatc
     8221 taagtacctg acttttttca taaaaggatt ttcgatttcc tcgattttat aaccgcaaat
     8281 ggtgcctgta attttacttg catttggatt aattttggcc tgctgaaaaa attcctgaaa
     8341 ggttgtttta ttgtctaaat gtttttgtaa ttctttaaca ctataacccg ttagccatgt
     8401 aataacctga ttcaattcct ctgaagtgcg attctttttt tgaatcttag aaatatataa
     8461 aggatatata gaagcaaaac tcatttttgc tacacgctca atattttttt ctgtgctatt
     8521 catatcaatt attttaaacg gactaattta ccttttcttt ttatcaggtt tttataatcc
     8581 cattgaatag ttatagattt ttttagccac cgctttaaat ccttaatatt tatttgttca
     8641 attgaagtat aaaatacaga agcatcttta aactttttgc catgcacatt caaaccttct
     8701 tcttcaaaat caattccact ccaaaacata agcctccagc ctagcttttg tttactgtat
     8761 ccaactattg gattttcatc aataaaccaa accgggtgtg catgccatat tttacattct
     8821 gctgttttta atactgtgtc aattgaatca gccaataaat cgcaaatttt tttgtcttcc
     8881 tgtgattgac tgttattgta agtctgaatt gatttttcca tatagagcaa aactaacaga
     8941 aactattgac agaatttaac aagtaaaatc agaaagtcat ttattcacga catgtacaag
     9001 aagatgcaaa atagagcttt gtagctttaa aagaatagtg ctttcagttc agttgcatcg
     9061 gcaggtttca tttttcctgc aaggattaag gcaagttgct tacgtcttaa tgcaccatca
     9121 aaacgttttt tttcttcttc gctttcagca atcaattcag gcactgcaat ggggctgcct
     9181 tgttggtcaa cagctacaaa ggtgtagatg gcttcattgc actttatttt atctcctgtg
     9241 gcattacgtt caacccaaac ttccataaaa acttccatgc ttgaagtaaa ggagcgcgat
     9301 acttttgcct gaatggtaac aatatcgccc aaacggatag gttgattaaa cgatacattg
     9361 tttacagaag ctgtaaccac tacacgtcca caatgacggt gtgcagctac ggcagaacaa
     9421 acatccatcc aatgcagcag gcggcctccc atcagattgc caagtacgtt ggtgtcgtta
     9481 ggaagaacaa tctcagtttg tatggttaaa ctctctttag cagtttttgc tttcataatc
     9541 tgattttttg cggctgcaaa tatctgaata attaaactta aaatgaacga atcaaagcca
     9601 cactgtggaa ttaaacctat ctttgcatcc acattggaaa acaattaaaa tgcaaacaaa
     9661 actaatagca ctgacagcca ttatcggaat gatgttaaac ggtaaaatgg cacattcaca
     9721 aaccccggtc aatatgagtg aaacaagaaa aattgaagtc acaggaactg ctgaaatgaa
     9781 agtaatacct gatgaaattt ttgttgtcat aaccattcag gaatatacag agaataagaa
     9841 gaagacagat ataaatgagg tgcgaaaaaa ttttcttgca gcatgcacaa aagccggtat
     9901 agctaaagaa aatatttcaa tacaaggaat gaatggctac aataacagtg gttggtactg
     9961 gcagcgcaaa aagaaaactc ccgattttgt tgcttcttca agttatataa ttaagtttaa
    10021 ttcaggtgag cagattgaaa gattaatgag tcagcttgat gacaaaggca cgcagaacat
    10081 gtacatcagt tccacttcac acagtaagat ggatgagttt aaaaaacaag taaaaacaga
    10141 agcattacag gcagccaaag ctaaggccac ctatctttgt gaaggaattg gagaaaaggt
    10201 tggcaaggta ttactgataa aagaagttga gggcacaaat tatgcagaac ctatgatgat
    10261 gaaaagccga atgatggaag cttcaatggc agattctaat tttgaacctt cgggacttga
    10321 attccaaaag ataaccatac agatggaaat attggcgcaa tttgccatac aataagcaac
    10381 acatgcagaa cagtaaaggc cgtaagcgcg gcaacagcga caagccgcaa gccggcagat
    10441 acaaatctga aggacgtcct tcaagaggaa aaaaattcgg aagtagcact gataaatcca
    10501 aagaggagtt tgtaaagtca ggaaacagtt attcaaaaaa taaattttca ggcaaacgca
    10561 ggtttgatgc tgatgagtcc ggctttgaaa gaaaacggaa gaattttgga agtgatgata
    10621 acgctggttt cgaaaaaaga gaaagaccat cgcgctttaa aaaagaaggt ggttttgagc
    10681 gagagaaaag aagttttaaa tcatcagata gtccacgccc ttacagaaaa agagacgaag
    10741 gttttgatgg tgaagaaaga ccatcgcgct ttaagaaaga aggtggtttt gagcgagaga
    10801 aaagaagttt taaatcatca gatagtccac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn gtccttacag aaaaagagat gaaggttttg aaggtgatga gaaaccaaga
    10981 tttaaaaaac gaagtgactt tggtggttca ggaaaatttg acaagtcaaa aagttttaaa
    11041 agaagagatg ataatgttga aggagaagat aagccaagac gatttagcaa atctttcgat
    11101 agtgatacaa gagagaaacg ttcttacgac agaaaagaaa gaagttcagg aagatttgat
    11161 aaaccgtatc gcaagaaaaa tgacaacaga aaatatagta aaaaagaaaa ggaatcatca
    11221 gaacaattgt ctgatggtct tgttcgatta aaccgttata ttgccaacag tggtatttgt
    11281 agcagacgcg aagcagatga gttgattact gcaggccttg tttctgtcaa tggtgaaatt
    11341 gttactacaa tgggaaccaa ggtttcgcag gatgatgtta tcaaatacaa tggtgaaact
    11401 atcagaaatg aaaagccaat gtatctgttg ctcaataaac caaaagatta catcaccaca
    11461 atggacgatc cgcaggacag acgtactgtt atgcatctta ttgccggtgc ttgtaaagag
    11521 cgtgtttatc ctgttggccg actcgacaga aatacaaccg gtgttttgct ttttaccaat
    11581 gatggtgatc tggcaaaaaa acttacacat cctagttatg aagtgcagaa aatttatcat
    11641 gtagagttgg acagaaattt aaaatcttcc gataaagaaa aaattaaaga aggtgtaatg
    11701 ctcgatgatg gaatggctac tgttgatgac atacaatacg atggtggatt taatgacaaa
    11761 aaacagattg gtgtagaaat acactcaggt aaaaaccgta ttgtaagacg aatatttgaa
    11821 tctatggatt acaatgttat taaattagac cgggtagttt ttgcaggctt gaccaagaag
    11881 gatattgcac gaggtaaatg gcgttttttg agtgagatgg aagtgaatgc tttgaagatg
    11941 gctacatcaa aggtgaagcc aaaggacaaa gagccacgca aaagaatttc gcgaagtaaa
    12001 taactatata taattaaact tgagtagtac tgcattgcat ggtttgatta ttgcttccgg
    12061 tatatggaga gcagtattaa tgacaccggg cggtgaatta ccttttaatt ttgaaacaag
    12121 agaggttaat agccgcagtg agatttttat aaaaaatggc aatgaagaaa ttgaagtaaa
    12181 tgaaatttct tatgccaatg attctttaat tattcaatta ccggtttatg attctgagtt
    12241 cagagtgaga gtaagcggtg acagcatgat tggcttttgg tataacaatt caagaaagaa
    12301 ttatgcaccc acaccattca aagcttttct tggagaaaag tatcgttttt ttaataacac
    12361 aaaatctgaa ataaaacttc ctgaaaaatg ggaatggaaa ttcagcccgg gtactaaaga
    12421 ttcatcgcaa gcaattgcat tgtttaaaca gaatggtaca gatgtgagag gaacactttt
    12481 aactgagtcg ggcgactatc gttttcttga tggatgtctt aataacgaca gtttgttttt
    12541 atcttatttt gacggagcat ttgcttatct ttttaaaggc tcaataaaga ataatatgat
    12601 gcagggtgtt ttttattcag gacatcattg gcagcaaccc tggcaagcag tagttaatga
    12661 aagtgcagaa ttgcccgatc cgtttaaact cacttttctg aagaatgata ctgcatcttt
    12721 ttcttttagt gcattgcgat tggacagcac agttttttca taccctaata aagatttaaa
    12781 aggtagggtg gttgttgttg agatgatggg aacatggtgc cctaactgta tggacgaaac
    12841 agcattttta tccgattatt attccagaaa taaaagccaa gacttaacca taattggttt
    12901 ggcttttgaa aaaacatctg acttcaggaa atctgtcggt aatgttaaac gagtgaaaga
    12961 aaaataccat gttcagtacg aaacactgat tgccggtaac agagaagggg ctgcatctaa
    13021 aatgagtatg ctcagtaaaa tcaatggcta tcctactacc atttttattg atcgaaaggg
    13081 caaagtcagg aaaatctata caggttttaa tggcccggct acaggcgcct atcatgaaag
    13141 gactaaggat gactttatcc gcatcatcac taccttgctt tccgaaaaat agctgttcac
    13201 ctatattttt ctgctttttg actacagatt ttttctgtta atcaaaaaaa ccacttgcaa
    13261 gtttcaggaa aaaggcttaa tttaagttac ttttttattc cctttatcct cgcagattct
    13321 ccaagatatt atgtcaagtg actataaaga aatatcacaa aatttagaat atttcaactt
    13381 tcgaaatatt ctaaatttaa tttcgagtag tttcctgcta ttactaagta ggcgcgagcg
    13441 tcctttccgc tcgtgccttt tatccttgtg gcttctggcc gtccgatagc tatcggactt
    13501 catagttcaa ccagctttaa atcggtaaat tgattagcac tgctatacac ataaaaatgg
    13561 ggctctgtaa ctattcgtgt ttctacagga ttgttgtgta tgtaatttac tttttgatca
    13621 ataaatttat tactgaata
//
LOCUS       DAORQT010000024        13320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_493_length_13320_cov_20.300641, whole genome shotgun sequence.
ACCESSION   DAORQT010000024 DAORQT010000000
VERSION     DAORQT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_493_length_13320_cov_20.300641"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK134_01855"
     CDS             <1..491
                     /locus_tag="PK134_01855"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37630.1"
                     /translation="TGLQPCTQDCHGDWGGTAFIDSCNACVGGNTGLQPCTQDCHGDW
                     GGTAYLDSCGICVGGYTGLWDCDTINAINIITWYNEINIYPNPLSGNDINIKAGNYSG
                     NISISITDLTGKLIFTSDCYMEKGKCLVNINPMPVDGLYLFKIQFNGTMKVVKRLSIF
                     GQ"
     gene            complement(496..954)
                     /locus_tag="PK134_01860"
     CDS             complement(496..954)
                     /locus_tag="PK134_01860"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRB37631.1"
                     /translation="MIKFTMRVLNLKVVRYFFSAASATIVDVGIYFLAFNYLFEKKDI
                     VFSFYTFSAPTVSLMLSYSCGLLTNFFLTKTLVFKESDLKGHHQFLRFVLVAIGVLFL
                     NYLLMKFLIRQMDWYPTIARAFSAVSIGLISFVVHKSFSFRVSATEEVED"
     gene            complement(1013..1978)
                     /gene="rfaD"
                     /locus_tag="PK134_01865"
     CDS             complement(1013..1978)
                     /gene="rfaD"
                     /locus_tag="PK134_01865"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRB37632.1"
                     /translation="MIVVTGAAGFVGSCLVKKLNEENFNDLILVDDFSNEHKNRNLEG
                     KKFTGKVDRRQFFKWLDENHQLTQFIFHIGARTDTTEFNKKIFDELNVEYSKKVWQKC
                     VEYGLPLVYASSAATYGGGEAGYDDDENRINQLKPLNPYGDSKNEFDKWALQQKEKPY
                     FWAGLKFFNVYGPNEFHKGRMASVIFHGFNQVQKDGVIRLFKSHRPEYKDGWQLRDFI
                     YVKDVTSVCFFLMNHRKNSGIYNLGTGKARSFYDLASSTFKAMDIECNIQFVDMPIDI
                     RDKYQYFTEAKMEKLSAIGYKEPFYSLEDGVSDYVCNYLLPAKYY"
     gene            complement(1982..2836)
                     /locus_tag="PK134_01870"
     CDS             complement(1982..2836)
                     /locus_tag="PK134_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRB37633.1"
                     /translation="MKDALKLAFSPCPNDTFMFHAWVEGLIDTQGHQFEIKMADVEEL
                     NQSAMKEDADITKLSFSAFAHVADRYELLTSGAALGHNCGPLLVSKHNFKWEELSQVK
                     IVIPGKYTTANLLLTMFAPDAKNKKELVFSDIESEVMNERADAGLIIHESRFTYHQHG
                     LKKIADLGELWENKYKCPLPLGCIAVKKSLPPEVKIQLNRLMHDSIAYAMKYPKASAD
                     FIREHAQEMDENVQQQHISLYVNQWSLELGEIGKKAIRRMMEEGTIAGLLPNTEVVFV
                     DELKKLVN"
     gene            2897..3850
                     /locus_tag="PK134_01875"
     CDS             2897..3850
                     /locus_tag="PK134_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRB37634.1"
                     /translation="MSFVLKTNQAAYLLDPDNDSLVLKRKGKWSSVYLGYRVEDKLPV
                     VIKKLHNPNEMQTQLRFQREALMGFNFDGVQHTLDAWKDESGYYIIKEFIDGLSLRQL
                     MQHDIKNRTQFSIKCILATLDILEKIHSSGIIHADLRPDNILILVNKRGNPDLLNPSI
                     RIIDFGLSINMSEPVATQRIPFSLLYSPPEQLLNLPELINPSTDLFSLGLTLYESVAK
                     YPPFFNENPEMIMHMQLNTAVAEHTRIHPLLLAFIRKATFKKQLRLPPSQLSIDEIRQ
                     TVAEGQQDRFTDCNQMKTILQEVAPNISETENKSFLKRLFN"
     gene            4009..4800
                     /locus_tag="PK134_01880"
     CDS             4009..4800
                     /locus_tag="PK134_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB37635.1"
                     /translation="MEFIYSQRWCELVFENRNKNYGAYHLRQNESSRILHSWLIAITI
                     IATAIFGIAISGGPSVELIQQYSDKTQQSEIIYNFNKVTPPPLPSASTSIAVKSNGNG
                     IPVVTDRKIQTIEPLITTPVTTVPTLNSTGSGTSIVPFSSGSGTTDAGTTVTTTSDFT
                     LMPEVFPEFPGGEEALIRYVQKNIIFPHDLIRRDISGTVYVGFIIDKSGHVSNIKILK
                     GIDNAPELSVEAIRVLEKSPVWKPGLQGGKPVAVAYTLPVTFTTK"
     gene            4900..5364
                     /locus_tag="PK134_01885"
     CDS             4900..5364
                     /locus_tag="PK134_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRB37636.1"
                     /translation="MGTLFTIILLILAGIVLLILEILILPGLIAGIIGALMILGGIWY
                     SFHELGNTAGTIVALSTLTVTLSTIYLAFRYNVWRRFSLQQSSDSKIERVDEMDIKSG
                     DHGKTLSALRPMGTAVFHNKKVEVQSLGEMIEANATVEVIEVLANKLIVKKI"
     gene            5383..6354
                     /gene="floA"
                     /locus_tag="PK134_01890"
     CDS             5383..6354
                     /gene="floA"
                     /locus_tag="PK134_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681040.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRB37637.1"
                     /translation="MASIPLLILVVAGIILFFLFLYFVPVNLWITATFSGVKVSILNL
                     ILMRFRKVPPSVIVNAMITATKAGLSVTIPDLETHYLAGGNVNNVIKALISADKANIP
                     LSFKMAAAIDLAGRDVADAVQLSVNPRVIDTPPVSAVGKNGIQLVVRARVTVRTNINQ
                     LVGGAGEETILARVGEGIVTTIGSAHDHKSVLENPDQISKTVLNKGLDAGTAFEILSI
                     DIADIDVGENIGAKLQMEQASADLKVAQAMAEERRAAAVALEQEMIAKAQEARAKVIE
                     AEAQIPMAMAEAFRSGKLGIMDYYKMENIQADTSMRESIGKPPKGNS"
     gene            6406..6479
                     /locus_tag="PK134_01895"
     tRNA            6406..6479
                     /locus_tag="PK134_01895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6440..6442,aa:Arg,seq:acg)
     gene            complement(6620..7246)
                     /locus_tag="PK134_01900"
     CDS             complement(6620..7246)
                     /locus_tag="PK134_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRB37638.1"
                     /translation="MELKKIFENNQKWIDRKLATDAQYFEKLCKGQSPEVLFVGCSDS
                     RVTAEELMGSQPGEVFVHRNVSNMIISIDLNMNAVVDYAVNFLKVKHIVICGHYFCGG
                     VKAAMQAQDLGILNPWLRNIRDVYRIHKIELNNIPDENDRYNRLVELNVQEQCINLIK
                     IASVQKAYKQRNLQVHGWVFDMQTGKLIDLKIDLPKELKDIMEIYDLG"
     gene            complement(7392..8561)
                     /locus_tag="PK134_01905"
     CDS             complement(7392..8561)
                     /locus_tag="PK134_01905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB37639.1"
                     /translation="MLKKLPVILVFIDWYKPAYKGGGIISCCDALVEHLKDRYCFKII
                     TRNADLGSTKQLEGIKSDCWVQRTENVEVFYLSKDKIGVFNMKSLIESTPHDLLYING
                     VFSFYFSLLPLIINKWRKLQHLTLVAPMGMLCLGALKFKNIKKTLYLKTAKLLNLYSD
                     ISWHLSSEYEKNDLEKVLGSEGEFYVAPYLFNPPQTEYKTAKKENNLLRMVFISRISP
                     KKNLLACFRYLSQVDLNVKVIFDVYGPVEDESYWNECLNQMNKLGKNIQCSYRGELKA
                     EEVIKTFQSYNVSILPTFSENFGQVIPESLLAACPVIISDQTPWNKLEEHSAGWVFSL
                     KEENRFVDIICTLAKMTNEQFKVYSEGAYQYAKGFLDSEQLVLESVRMFDAKLNQ"
     gene            complement(8767..13062)
                     /gene="rpoC"
                     /locus_tag="PK134_01910"
     CDS             complement(8767..13062)
                     /gene="rpoC"
                     /locus_tag="PK134_01910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRB37640.1"
                     /translation="MAAKRDNKIKSNFSRIIISLASPEHILEQSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGLPTKKLDLIIYYERYVVIQAGIKAQDGVNYLDFLT
                     EEEYLNILDNLPKENQHLDDTDPNKFIARMGADALYDLLSRLDLDELSYKLRHQASNE
                     TSQQRKNEALKRLNVVEAFRNANQNIENRPEWMIVKVVPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLLEIKAPDVILRNEKRMLQEAVDSLFDNSRKVNAVKTES
                     NRALKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPEMKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSAKKIVDRKDAIVWDILENVLKGHPVLLNRAPTLHRLGIQA
                     FQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAILEAQMLMLASHNILNPA
                     NGAPITVPSQDMVLGLYYMTKGKKSTDKEKVKGEGSVFYSPEEVTIAFNEGRVDLHAY
                     IRVKTFIKENDEIVKRVIETTVGRILFNEVVPRQAGYINDVLTKKTLRDIISQVLKTC
                     GIAQTARFLDDIKDLGFKMAFKGGLSFNLNDVLIPGAKAELVTKAQSEVEEVRNSYNM
                     GFITNNERYNQIIDIWTRTNTRITETLMKQLIADKQGFNAVYMMLDSGARGSKEQIRQ
                     LGGMRGLMAKPQKSGASGGEIIENPILSNFKEGLSILEYFISTHGARKGLADTALKTA
                     DAGYLTRRLVDVAQDVIITEPDCGTLRGLSETALKNQEEVVETLYDRILGRVSCHDIY
                     HPLTGELVVRAGDEIKEDVATQIEESPLESVEIRSVLTCESKQGVCAKCYGRNLATGR
                     MVQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGVASKGASESRHEAKFGGVIELEEMRT
                     LPYKEGDNKYEVVVGRSGEFRIVDPTTKAILATNNIPYGSHLFVKNGDTLKKGDVICE
                     WDPYNAVIICEADGKIGYEHLEEGVTFREESDEQTGYKEKVVIDSRDKTKNPVIKILN
                     DKKEVVKAYNIPVGAHIVVNEGQKIKAGNVLVKIVRASGKTGDITGGLPRVTELFEAR
                     NPSNPAVVSEIDGVVAYGGIKRGNREIIITSKEGDVKKYLVPLSKHILVQDNDFIRAG
                     APLSDGAISPSDILAIKGPSQVQEYLVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVV
                     IEDAGDTRFLERESVNKIEFMEENDWIMDKMVVTEPGDSEYRPGQIITLRKLRDDNSM
                     LKRKDQKEIEARNAIPATSSPLLQGITQASLHTKSWISAASFQETTKVLNEAAINGKV
                     DYLNGLKENVIVGHLIPAGTGLREYERLIVGSQEEYDLLMASKESSSEQPINM"
     gene            complement(13132..>13320)
                     /locus_tag="PK134_01915"
     CDS             complement(13132..>13320)
                     /locus_tag="PK134_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37641.1"
                     /translation="EAFGASNILQELLTVKSDDVVGRAKAYEAIVKGDNMPTPGVPES
                     FNVLLHELRGLGLKITLD"
BASE COUNT         4116 a         2726 c         2186 g         4292 t
ORIGIN      
        1 acacaggtct tcaaccttgc acacaagatt gtcacggtga ttggggtggt actgctttca
       61 ttgattcatg caacgcatgc gttggtggaa atacaggtct tcaaccatgc acacaagatt
      121 gtcatggtga ttggggtggc actgcctatt tggattcttg tggaatatgt gtaggtggat
      181 acacagggct ttgggactgc gacactatca atgctattaa tatcattaca tggtataatg
      241 aaataaacat ttaccctaat ccactcagtg gaaatgatat caatattaaa gcaggtaatt
      301 attctggaaa tatctcgatt tcaataactg acttaaccgg taaattaatt ttcacatcag
      361 attgttatat ggaaaaaggt aagtgtttgg taaatatcaa tccaatgcct gtggatgggc
      421 tttatctttt caaaatccaa tttaatggaa caatgaaagt agtgaaacgt ctctctatat
      481 tcggacagta gcagattaat cttctacttc ttctgttgca cttactctga acgaaaagct
      541 tttgtgaaca acaaaactta tcagtcctat actcactgca gaaaaagcac gtgctattgt
      601 aggataccaa tccatctgac gaataaggaa tttcatcagc aaataattca aaaacaagac
      661 accaatagct accaatacaa aacgcaaaaa ctgatgatgt ccttttaaat ctgattcttt
      721 aaagactaaa gtttttgtaa gaaaaaagtt cgttaacaat ccgcatgaat aagacaacat
      781 tagagaaact gttggtgcag aaaaagtata aaatgaaaag acaatatcct tcttttcgaa
      841 caaataatta aatgccaaaa aataaattcc tacatctaca attgttgcag atgctgctga
      901 gaaaaagtac cgaaccactt tcaggttcag aacacgcata gtgaatttta tcatcagggc
      961 gctaaaatag gttttcctga tttgctgtta atcattgttg ataaactatg aattaataat
     1021 attttgcggg caacagataa ttacatacat aatctgacac accatcttca agcgaataga
     1081 aaggttcttt gtatccgatt gcactaagtt tttccatttt tgcctctgta aaatactgat
     1141 atttatctct aatgtcaatt ggcatatcaa caaactgaat attacactca atatccattg
     1201 ccttgaatgt acttgatgct aaatcataaa aactccttgc ctttccagta ccaagattgt
     1261 aaataccgga atttttacga tgattcatca gaaagaaaca gactgatgta acgtccttca
     1321 catagataaa atcacgcaat tgccagccat ctttatactc agggcgatgc gatttaaata
     1381 atcgaataac accatctttt tgcacctgat taaatccatg aaaaataact gatgccatcc
     1441 tgcctttatg aaattcattt ggcccatata cattaaaaaa ttttaacccc gcccagaaat
     1501 aaggcttttc tttttgttgc agtgcccact tatcaaattc gttctttgaa tctccataag
     1561 gattcaatgg ttttaactga ttgattctat tctcatcatc atcatagcct gcttctcccc
     1621 caccataagt tgcagctgat gatgcataaa caagcggcaa gccatattca acacacttct
     1681 gccaaacctt tttactgtat tcaacattta actcatcaaa aatctttttg ttaaattctg
     1741 ttgtatcggt ccgcgcacct atatgaaaaa taaactgtgt aagttgatga ttttcatcta
     1801 accacttgaa aaattgcctc ctatctactt tgcctgtaaa tttttttcct tcaagatttc
     1861 tattcttatg ttcattgctg aaatcatcta caagaatcaa atcattaaaa ttctcttcgt
     1921 tgagtttctt taccagacaa cttccaacaa atcctgctgc tcctgtaact actatcattt
     1981 tctaattaac taattttttt aactcatcta caaatacaac ttctgtattt ggtaataatc
     2041 cggcaatagt gccttcttcc atcattcttc tgattgcctt ttttcctatc tctcccagct
     2101 ctaacgacca ttgattaaca tacaatgaaa tatgttgctg ttgcacattc tcatccatct
     2161 cctgtgcatg ctctctgata aaatcagcag aagccttcgg atatttcatt gcataagcaa
     2221 tgctatcatg cattaatcta tttaattgaa tttttacttc cggtggcagg ctctttttta
     2281 ctgcaataca acccaacggt aatgggcatt tatatttatt ttcccaaagt tctcccaagt
     2341 cagcaatttt tttcaatcca tgctgatgat aagtaaaacg actctcatgt atgatcaatc
     2401 ccgcatcggc tctttcattc atcacttcac tttcaatatc tgaaaaaacc aattcttttt
     2461 tattttttgc atccggtgca aacattgtca gcaataaatt tgctgttgta tacttcccgg
     2521 gaataacaat ttttacttgt gataattctt cccatttaaa attatgctta cttactaaaa
     2581 gcggtccgca attatgtcca agtgcagctc ctgaagtcag caattcatac ctgtctgcaa
     2641 catgtgcaaa ggcgctaaaa ctcaatttgg taatatctgc atcctctttc attgctgact
     2701 gatttaattc ttcgacatct gccatcttaa tttcaaactg atgaccttga gtatcaatta
     2761 aaccttctac ccatgcatga aacataaagg tgtcattggg acagggagaa aatgctaatt
     2821 taagtgcatc tttcatgcaa caaatttaca taaagcttgt ttattaattt agcccaccat
     2881 aaatattctc tgaatcatgt cattcgttct gaaaaccaat caggcagcct accttctcga
     2941 ccccgataat gatagccttg tgctgaaacg caaaggaaaa tggagttctg tatatttagg
     3001 ttatcgtgta gaagataaat taccggtggt gattaaaaaa ttacacaacc cgaatgaaat
     3061 gcaaacacaa ttaaggtttc agcgtgaagc attgatgggc tttaactttg atggtgtaca
     3121 gcatacactc gatgcatgga aagatgaaag cggctattat ataattaaag aatttattga
     3181 tggcctttca ttgcgtcaac tgatgcaaca tgacataaaa aacagaacac aattcagtat
     3241 caaatgtatt cttgccacac tcgatattct cgaaaaaatt catagcagcg gaattattca
     3301 tgctgattta cgacctgata atattctgat tcttgttaac aaaagaggta atcctgattt
     3361 actcaaccca agtattcgaa ttattgactt cggactttcc atcaatatgt cagagcctgt
     3421 tgcaacacag cggatacctt tttcacttct ctactcacca cctgaacagt tattaaattt
     3481 accggaactc ataaatccat ctacagattt attcagcctt ggacttacat tatatgaatc
     3541 tgtggctaag tatcctccgt tttttaatga gaacccggag atgataatgc acatgcagtt
     3601 aaatactgca gttgcagagc atacaagaat acaccctttg cttttagctt ttatacgtaa
     3661 agctactttc aaaaaacaac tccgtcttcc tccatcacaa ttatcaattg atgaaatacg
     3721 gcaaacagtt gctgaaggtc agcaagaccg atttacagat tgcaatcaaa tgaaaacaat
     3781 tttacaggaa gtagcaccaa atatttctga aactgaaaac aagagttttc ttaaaaggct
     3841 atttaattga ttttttcatt ctgttaaaag aaattaatgt aagaatatat acatttttaa
     3901 cttgaagaag gaatcaaatt ttaccgtcat tagaatgtaa aatagttttc agctaaaaat
     3961 aatatggaat gttgcagcgt catacatcta aaagataaaa gcaacattat ggaattcatt
     4021 tattcacagc ggtggtgcga actggtgttc gaaaaccgca acaaaaatta tggtgcatat
     4081 catttaagac aaaatgaatc aagccgcatt ctacactctt ggttgatagc cattacaatt
     4141 attgcaacag caatatttgg aattgcgata tcaggtggtc catctgttga acttattcag
     4201 caatactcag ataaaactca acaatctgaa ataatttata acttcaacaa ggtaacacct
     4261 cctccactac cttctgcatc tacatcaatt gcagtaaagt ccaacggtaa cggcattccc
     4321 gtagtgactg atagaaaaat tcaaactatt gaacctttaa taaccacacc tgttactacc
     4381 gtacccactc ttaattcaac tggtagcggc acttctattg ttcccttttc atctggcagt
     4441 ggcactacag atgcaggaac tacagtcacc acaacttcag atttcacatt aatgcctgaa
     4501 gtatttcctg aatttccggg aggtgaagaa gccttaattc gttacgtgca aaaaaatatt
     4561 atttttccac atgacctaat caggcgtgat atcagtggaa ctgtttatgt tggatttata
     4621 attgacaaaa gtggtcatgt cagcaatatc aaaattctta aaggtattga taacgcacct
     4681 gaattgtcag ttgaagcaat cagagtgctt gaaaaatctc cagtttggaa accgggacta
     4741 caaggaggta aacctgtggc agttgcatat acattaccgg tgacatttac taccaaataa
     4801 tttacaattc agcaaaaatc agcataaaaa atattcgtgt tttttaatag gggctgttgt
     4861 tttttagcaa cggccttttt actatgtttg tcaccgaaaa tgggaacctt atttacaatc
     4921 attttactga tacttgcagg tatagttctg ttgattttgg agatattgat tctgccggga
     4981 ttaattgcag gtatcattgg tgcattaatg attttgggtg gcatctggta ttcctttcat
     5041 gaactgggaa atactgcagg caccatcgtt gcactaagta cacttactgt aacactatct
     5101 accatttatc ttgcatttcg ttacaatgtt tggcgcaggt tcagcctgca acaatcaagt
     5161 gattctaaaa ttgagcgtgt tgatgaaatg gatataaaat ccggagatca tggaaaaaca
     5221 ctttctgcat tgagacctat gggcacagca gtatttcata ataaaaaagt agaggtgcaa
     5281 tcacttggcg aaatgataga agcaaacgct acagttgaag tgattgaagt attggcaaac
     5341 aaacttattg taaaaaaaat ataaacttaa aaattaaaaa aaatggcaag tattccgtta
     5401 ctcattctgg ttgttgcagg tattatcctg ttctttttgt tcttgtattt tgttcctgtt
     5461 aacctttgga taactgcaac attctccggt gtgaaagtca gcatcctgaa cttaatcctg
     5521 atgcgtttca gaaaagtacc accaagtgta attgttaatg ctatgattac tgcaactaaa
     5581 gcaggtctaa gtgttaccat tcctgattta gaaacacact atctggcagg aggaaatgta
     5641 aataatgtta ttaaagcact aatttctgct gataaagcaa acattccctt atcatttaaa
     5701 atggcagcag caattgatct ggccggtcgc gatgttgcag acgctgttca gctttctgta
     5761 aatccacgag ttattgacac ccctcctgtt tctgctgtgg gcaaaaacgg aattcaattg
     5821 gtagtacgtg ctcgtgttac cgttcgcacc aacatcaatc agttagttgg tggtgcaggt
     5881 gaggaaacca ttcttgcacg tgttggcgaa ggtattgtaa ctactattgg ttcggcacat
     5941 gaccataaaa gtgtattaga aaatcccgat cagatttcaa aaactgtatt aaacaaaggt
     6001 cttgatgcag gcacggcatt tgaaatttta tctatagaca ttgccgatat tgatgtaggt
     6061 gaaaacattg gagcaaaact tcagatggaa caggcttctg cagacttaaa ggtagcacag
     6121 gcaatggcag aagagcgcag ggctgcagcc gtagctttag aacaggaaat gattgccaaa
     6181 gcacaagagg caagagccaa agttattgaa gccgaagcac aaattcctat ggctatggca
     6241 gaagcttttc gtagtggtaa attaggcata atggactatt acaaaatgga aaacattcag
     6301 gccgacacat ctatgcgtga gtcaatcggt aagccaccaa aaggaaattc ataatttctc
     6361 ttttgtaata agctaatttg gttatctttg cgccactaaa aaaatggttc cgtagcttaa
     6421 ctgaatagag catctgacta cggatcagaa ggttgggggt ttgaatccct ccgggaccac
     6481 ttaatataaa gcgaatcagt aatggttcgc ttttctgttt tctatttatg gctactgaat
     6541 gtactgcagt taagaaaatt agaaatttaa aatatgagga aatcttacca tgaatgtttg
     6601 atattcttaa ggcaactacc taccctaaat catagatctc cataatatct ttcaactctt
     6661 ttggtaaatc aattttcagg tcaattaatt tgccggtttg catatcgaac acccatccat
     6721 gtacttgcaa attgcgttgc ttatatgctt tttgaaccga tgcaatttta atcagattaa
     6781 tgcattgttc ctgaacattc aactccacta agcgattata acgatcgttt tcatcaggaa
     6841 tattattcaa ttctatttta tgaatacgat atacatcacg aatatttctt agccatggat
     6901 tgagtatgcc taaatcctgt gcctgcatag cagcctttac tcctccacaa aaataatggc
     6961 cacagattac gatgtgtttc acttttaaaa aattcacagc ataatctaca acagcattca
     7021 tgtttaaatc aatgctgata atcatatttg aaacattgcg atgtacaaat acttcaccgg
     7081 gctgcgatcc cattaactcc tctgctgtta ccctgctgtc agaacaacct acaaataaaa
     7141 cttcaggact ttgacctttg cacaattttt caaaatattg cgcatcagtg gccagtttcc
     7201 tatcaatcca tttctgatta ttttcaaata tttttttaag ttccattgta caaatttaat
     7261 aataaaaaat attgttacct ccttgcttga attttaacag caataaatcc aactatcatt
     7321 ctggataaac taacgcatga atttgtcatc atatatttca ttatacatta aaaataaaat
     7381 atagaaaaca attattgatt cagcttagca tcaaacattc ttacagactc caacactaat
     7441 tgttcactat ctaaaaagcc ttttgcatat tgataagctc cttccgaata aaccttaaat
     7501 tgctcatttg tcattttcgc aagagtgcat attatatcca caaacctatt ttcttctttt
     7561 aaagaaaaca cccacccggc agaatgctct tccaatttat tccatggtgt ttgatcgctt
     7621 ataattacag gacatgcagc aagtaaagat tccggtataa cctgcccaaa gttttcgctg
     7681 aaagttggta aaatagaaac attataactt tgaaatgttt ttataacctc ttcagctttc
     7741 aattctccac ggtagctaca ttgaatgttt tttccaagtt tattcatctg attcaaacat
     7801 tcattccaat aagattcatc ttctaccggg ccataaacat caaaaatcac tttcacattt
     7861 aaatcaactt gtgataaata acgaaagcaa gctaaaagat tttttttggg tgatatccgt
     7921 gaaataaaaa ccattcgcag caaattattt tccttctttg ccgttttata ttctgtttgt
     7981 ggcggattaa ataaataggg agcaacataa aattctcctt cgcttcccaa cactttctca
     8041 aggtcattct tttcatattc tgatgacaga tgccacgaaa tatccgaata caaattaagc
     8101 aacttagctg ttttaagata aagagtcttt ttaatattct tgaatttaag tgctcctaaa
     8161 cataacatcc ccattggagc aaccaatgtt aggtgttgaa gtttacgcca cttattgatt
     8221 attaaaggca ataaactaaa ataaaacgaa aataccccat taatgtataa taggtcatgt
     8281 ggcgtacttt caatcaatga tttcatattg aaaacaccaa tcttatcttt tgaaagataa
     8341 aatacttcta cattttcagt acgttgaacc caacaatcac ttttaattcc ttcaagctgt
     8401 ttggtggaac ccaagtctgc gtttcgtgta attattttaa agcaatatct gtctttcaga
     8461 tgctctacta atgcatcaca acaggaaatt atacctccac ctttgtatgc tggtttatac
     8521 cagtcaataa acacaaggat aacaggtaat ttttttagca tttttctaat ctctaaaact
     8581 aacctaccaa tggttattta catttgggtt aacaaaatac gaaaataaaa attttatcta
     8641 ttacatttta aaattgtata atcaaattag agcatacaat ttcaaaaact aactacctgt
     8701 taaaagtaaa acacctttca gacttgggtc tgaaaggtgt ttgtttaaat atgctctatc
     8761 taaattttac atattaatag gctgctcgct gcttgattct tttgaagcca taagcaagtc
     8821 atactcttct tgtgagccaa caatcaaacg ctcatattca cgtaaccctg ttcctgcagg
     8881 aattaaatga ccaacgataa cgttttcttt caagccgttc agataatcaa cttttccatt
     8941 aattgctgct tcattaagaa ctttagttgt ttcctggaat gatgctgccg atatccaact
     9001 cttagtatgc aatgatgcct gagtgatacc ctgcaacaat ggtgatgatg tagcaggaat
     9061 agcattacgt gcttcaatct ctttttgatc tttgcgctta agcattgagt tgtcgtcacg
     9121 taatttacgt aatgtaataa tctgacctgg acgatattct gaatcaccgg gttctgttac
     9181 caccatctta tccataatcc aatcgttctc ctccataaac tcaatcttgt ttacagactc
     9241 tctctcgagg aagcgtgtat ctcctgcatc ttcaattact accttacgca tcatctgacg
     9301 aacgataact tcaaagtgct tatcgttaat cttcacaccc tgcaaacgat acacttcctg
     9361 cacctcgttt actaagtatt cctgtacttg cgatggtcct ttgattgcaa gaatgtcgga
     9421 tgggctgatt gcaccatcag ataatggagc acctgcacgg ataaagtcgt tgtcctgtac
     9481 aagaatgtgt tttgataacg gaacaagata tttctttaca tctccttctt tagatgtgat
     9541 gataatctca cggttaccac gtttaattcc gccataagct actactccgt caatctcact
     9601 tactacagca ggattcgatg gatttcttgc ttcaaacaat tctgttacac gtggcaaacc
     9661 accggtgatg tcaccagtct taccacttgc acgaacaatt ttcaccaata catttcctgc
     9721 cttaattttc tgaccttcat tcaccacaat gtgagcacca acaggaatgt tatatgcttt
     9781 tacaacttct ttcttgtcgt taagaatctt aataacaggg ttctttgttt tatcgcggct
     9841 gtcaatgaca actttctctt tgtaacctgt ttgctcatca gactcttcac ggaatgtaac
     9901 accttcttca agatgttcat agccaatttt accatcagct tcgcaaatga ttaccgcatt
     9961 gtatggatcc cattcgcaaa tcacatcgcc ttttttcaaa gtgtcgccat ttttaacaaa
    10021 cagatgtgat ccataaggaa tgttgttcgt tgctaatatt gctttagttg ttgggtcaac
    10081 aattcggaat tcacccgaac gaccaacaac aacttcatat ttattatcgc cttctttata
    10141 aggcagcgta cgcatttctt caagttcgat aacaccacca aacttcgcct catgtcgtga
    10201 ctctgatgca ccttttgatg caacaccacc cacgtggaaa gtacgaagtg taagctgtgt
    10261 acccggctca ccaatggact gtgctgcaat aacaccaaca gcctcacctt tctgaaccat
    10321 gcgtcctgta gctaagtttc tgccatagca tttagcacat acaccttgtt tcgattcgca
    10381 ggtcaatact gatctgattt ctacggattc taatggtgat tcttctattt gtgttgcaac
    10441 gtcctctttt atttcatcac ctgcacgaac aaccaattca ccggttaatg ggtgatagat
    10501 atcgtgacac gatacacgtc caagaattct gtcatataat gtctcaacaa cttcttcctg
    10561 atttttcaat gctgtttcag aaagaccacg taatgttccg caatcaggtt ctgtgatgat
    10621 aacatcttgt gctacgtcaa caagccttct ggtcagataa cctgcatcgg cagtctttaa
    10681 cgctgtatcg gcaagacctt tacgcgcacc gtgtgttgag ataaagtatt caagaattga
    10741 taatccttct ttaaagtttg aaagaatcgg attctcgata atctcaccac ctgatgcacc
    10801 tgacttttgc ggctttgcca tcaatcccct cattccgcct aactgacgaa tctgctcttt
    10861 agaaccacgc gcaccggaat caagcatcat atacactgcg ttgaaacctt gtttatcagc
    10921 aattaattgc ttcatcaacg tttctgtaat acgtgtattg gtacgtgtcc agatatcaat
    10981 aatctgattg taacgctcgt tattggtaat gaatcccatg ttgtagctgt tacgaacctc
    11041 ttctacttct gactgtgctt tagtaaccaa ttcagctttt gcaccaggaa tcaatacatc
    11101 gttaaggttg aaagaaagac cacctttaaa tgccatctta aatccaagat cttttatatc
    11161 atcaaggaaa cgtgctgtct gcgcaattcc acaggtcttc aatacctggc tgatgatatc
    11221 acgcaatgtc ttttttgtaa gtacatcgtt aatatatcct gcctgacgtg gaaccacttc
    11281 gttgaacaat atacgtccaa cagtggtttc tatcactcgt tttacaattt catcattttc
    11341 tttgatgaat gtttttactc ttatgtatgc atgtaaatca acacggcctt cattgaacgc
    11401 aatggtaacc tcttcaggag aatagaaaac agaaccttct ccttttactt tttctttatc
    11461 tgttgatttc ttacccttag tcatgtaata caagccaaga accatgtcct gtgaaggaac
    11521 tgtaattggt gcaccattgg cagggttaag aatgttgtgc gatgcaagca tcagcatttg
    11581 tgcttcaaga attgcagcgt ttccaagggg aacgtgaaca gccatctggt caccgtcaaa
    11641 gtcagcgttg aatgctgtac acaccaatgg atgtaactga attgcttttc cttcgataag
    11701 ttttggctgg aatgcctgaa taccgagacg gtgaagtgta ggagcacggt taagtaatac
    11761 aggatgtcct tttaatacgt tttcaagaat atcccaaact atagcatctt tacgatctac
    11821 aattttctta gcagacttaa ctgtctttac aattcctctt tcaataagtt tacggataat
    11881 aaatggcttg aataattctg ctgccatatc tttcggtaat ccgcactcat gcaatttcat
    11941 ttcaggacca acaacaatta ccgaacgtgc agaataatca acacgtttac caagaaggtt
    12001 ctgacggaaa cgtccttgtt taccttttaa tgaatctgaa agtgatttta atgcacggtt
    12061 tgattcagtt ttaacggcat tcactttgcg tgaattatcg aaaagagaat ctactgcttc
    12121 ctgcaacata cgcttttcat tacgcaaaat cacatcagga gctttgattt caagcaatcg
    12181 cttcaaacgg ttgttacgaa taattacacg tctgtaaagg tcattcagat cggatgttgc
    12241 aaaacgtccg ccatccaatg gaaccaatgg acgaagttcc ggtggaataa caggaacaac
    12301 ttttactatc atccactcag gacggttttc aatattctga ttcgcattac ggaaagcttc
    12361 aactacgttg agtctcttta atgcttcgtt cttacgttgc tgcgaagttt cattgcttgc
    12421 ctgatgacgt agtttatagg ataactcatc aaggtcgagg cgtgaaagca ggtcatacaa
    12481 agcatctgct cccattcttg caataaattt attcggatca gtatcgtcaa gatgctgatt
    12541 ttcttttgga aggttgtcaa gaatattcag gtactcttct tcggtgagga agtcaagata
    12601 gtttacacca tcctgtgctt ttatacctgc ctgaattact acataacgtt cgtagtaaat
    12661 aatcagatct aatttctttg taggtaatcc taatagataa ccgattttat taggtaaaga
    12721 acggaaatac cagatgtgtg caacaggaac aactaactgt atgtgtccca tgcgctcacg
    12781 tctaactttc ttttctgtta cttctacacc gcaacggtca caaacaattc ctttataacg
    12841 gatacgcttg tacttgccgc aatgacattc ccagtctttt acaggaccga aaatgcgttc
    12901 gcaaaacaaa ccatcacgct ctggtttata cgtgcggtag ttaattgttt ctggtttcag
    12961 cacttcaccg cttgactgct ccagaatgtg ttccggtgaa gctaagctga tgatgatgcg
    13021 tgaaaaatta ctttttattt tattatcgcg ttttgctgcc attttgtttt tgttgaataa
    13081 tttttatgat agaaaagtta tgtgcagagc aaaaaccctg cacatgttct tttaatctaa
    13141 tgttattttt aaacctaatc cacgcaattc atgcagtaat acattgaacg actcaggtac
    13201 tcccggtgta ggcatgttat ctcctttaac aattgcttca taagctttag ctcttccaac
    13261 tacgtcatca gacttaactg tcaacaattc ctgaagaatg tttgatgcac cgaatgcttc
//
LOCUS       DAORQT010000025        12845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_529_length_12845_cov_8.388428, whole genome shotgun sequence.
ACCESSION   DAORQT010000025 DAORQT010000000
VERSION     DAORQT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_12845_cov_8.388428"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="PK134_01920"
     CDS             complement(<1..1544)
                     /locus_tag="PK134_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRB37642.1"
                     /translation="MSEVRNPQVAIATEIYTEDSVLIGKYYHENRTPVSFDSISTYAV
                     QALVATEDVRFYKHFGLDLYALISGAYSTAKGDERGASTITQQLAKNMYSTRKKVNQG
                     LLQHIPLVGTLVSKSKEWITSVKLEMFYSKDEILEMYFNTVDFGNNWFGIKVAAQNYF
                     SKQPSELNLQESATLIGLLKATSSYNPLNNKKRSLARRNIVLSQMQKYGYISQAAFDS
                     ISPMPLNLKRPTNNPATKDSYLRQLIAKIIKSYCDENNLNINEDGLKIYTTINSHLQN
                     YAELAVKDHLRKVQKQFDEHWGKQNPWRDDNGREILNFANTAVAFTQSYDRLKKQFPS
                     APDSVNFYLNKKKSMKVFTYNGAKDTVMSTLDSLKYYARLLNAGTMSLNPLTGEIKAY
                     VGGVDFNFFKFDHVTQSKRQPGSTFKPFAYLAAIEDTFSPCSRLDDVPVSIEYDGGQI
                     WQPYNSTGAFTYSSKTLRRALAQSVNSITAQIAELVGWDKIVECAHRAGVKSRLDTVP
                     SICLGT"
     gene            1853..>2223
                     /locus_tag="PK134_01925"
     CDS             1853..>2223
                     /locus_tag="PK134_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRB37643.1"
                     /translation="MSLTEQFKEVVNKEYQFKGDSIVLGAPVLKGETLTGSFIKAPLK
                     TFNRHGLIAGATGTGKTKTMQGIAESLSAKGVSCLLMDVKGDLSGLASPGTTNPKIDE
                     RYQKLGLTWKPLQFPVEFLTLS"
     assembly_gap    2224..2239
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2240..3405
                     /locus_tag="PK134_01930"
     CDS             <2240..3405
                     /locus_tag="PK134_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRB37644.1"
                     /translation="KGVRLRATVSEFGPVLFSKILDLNDTQQGVVSLIFKYCDDKQMP
                     LLDLKDFKKVLQFLTNEGKDEIKADYGAISSASASTIIRKVIEIEQQGAEKFFGEKSF
                     EVEDLLGIDENGNGKLNIIRLTDIQDKPKLFSTFMLSLLAEVYSTFPEVGDPDAPKLV
                     IFIDEAHLIFNNASKVLLDQIETVIKLIRSKGIGVFFVTQSPEDIPAAVLAQLGLKVQ
                     HAFRVFTANDRKALKLIAENYPLSDYYKVDELLTTLGIGEAAITVLNEKGVPTPIAHT
                     LLCTPASRMDILTPDELTKSISKSRLTTKYNETLDRESAFEVLSKKLETQVAENTKAT
                     KAAEAKEEPSQLDQVLNSSTGKQVMRTVTSVLTRSLLGALGLGATRRKKSGWL"
     gene            3424..4248
                     /locus_tag="PK134_01935"
     CDS             3424..4248
                     /locus_tag="PK134_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRB37645.1"
                     /translation="MTSPKSEWKYDLISTIFPSKFLKREIRIDYYLPAQYSKNTGVLF
                     MLDGQDAELLHLKDTLQSLTQLKKIKPIIVIAIHANKNRLQEYGVVGKADYLKRGNKA
                     KQFSKFILDELIAHCSSFTGIKFTPSKTAIAGFSLGGLTAFDLAWNHPSVFETVGVFS
                     GSFWWRKKSYEDGYTDDDRIMHQVVKKSKSAPAIKCWIQTGTKDETADRNKNGVIDSI
                     DDARDLIFEMKQKGVPEKNIQYVEIPEGQHNQITWSAALPLFLCWAFGNKRKVDIY"
     gene            complement(4249..5625)
                     /locus_tag="PK134_01940"
     CDS             complement(4249..5625)
                     /locus_tag="PK134_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRB37646.1"
                     /translation="MRYHFIAIAGSAMHNLALALHQLGHEVSGSDDEIVEPAKTRLAK
                     SGILPEKTGWFPEKINADIDAVILGMHARADNPELAEAIQKNIKVFSYPEFLYEHSKN
                     KTRVVIGGSHGKTTITSMILHVLKTSGNNFDFMVGAQLEGFDTMVKLSNDAPIIVLEG
                     DEYLSSPIDRRPKFHLYHAQIGLISGIAWDHINVFPTLEIYEEQFRIFAREIPRDGKL
                     FYCMNDDAVRKICEAENVVADRKGYSIPKHVIRNGKTFIYDDYNKEVPLQIFGEHNLM
                     NLEGARMICNSIGVNNEVFYNAIKSFKGAARRLELLEENDQFAVFRDFAHSPSKLKAT
                     LEAVRRQFPERKLIACMELHTFSSLNENFLDEYKGTMADADVAVVYFNPKTIEHKKLP
                     PISSEKIFQSFDRSDIKVINDSMQLINFLNTEDKQQVVYLMMSSGTFDGLNLKSFAES
                     LNTSHQKV"
     gene            5763..>6549
                     /locus_tag="PK134_01945"
     CDS             5763..>6549
                     /locus_tag="PK134_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRB37647.1"
                     /translation="MKAKILDLYIIKKFLGTFLFAIGLIICIAVVFDISEKVDDFIER
                     HAPLKAIAFDYYLNFIPYFANLFSPLFVFISVIFFTSKMAGQTEIIAILNSGISFNRF
                     LRPYIISATVIAIASFYLNNWVIPHANLVRLQFENIYIKNPYVYRGRNIHRQIKPGEF
                     VYFESYDNKANTGFMFSIEKFEGGKLVTKLMADRITWDSLTQKWSIENYTIRHINGMT
                     EELKKGIRLDTTLSIHPSEFNRRMNFIEAMDFSELNSYIKEEKS"
     assembly_gap    6550..6559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6577..6879
                     /locus_tag="PK134_01950"
                     /pseudo
     CDS             6577..6879
                     /locus_tag="PK134_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(6930..8069)
                     /locus_tag="PK134_01955"
     CDS             complement(6930..8069)
                     /locus_tag="PK134_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37648.1"
                     /translation="MARKLKLRQRQKMSIMYKAIFVTAGTSIMGLAVYLTVVMNTSNV
                     TDSRAGALTNMIQGYDVNNGEVICAFDWNGDDPTKASKGPDAIKVSPFAVVMSEGVEG
                     TNGLSAGKGKDGIDLTIPTDNYFNTDGIDISFDYKRLDDNCDFISRGRYFNFGMKNGK
                     LIIAYRVNLEKGRSYSVNETTNYELVKDNDFRNYRFVYNPNTGKGEIMVDDVVVWNYQ
                     GPKERSMFWKNSDNIVIAKNMNGGGTDKTILDNFIIRSTTHVQQLPITLLSFQAEARD
                     HHVMVSWYTSRETDIDSFVVERSVDAIEYQKIGAIKANGNSEVLRAYAFVDKAPLENK
                     IAYYRLMPSNKPLKSIAVPIIGYRYRMNHPETAFNPADYPADTVK"
     gene            complement(8167..>10859)
                     /locus_tag="PK134_01960"
     CDS             complement(8167..>10859)
                     /locus_tag="PK134_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37649.1"
                     /translation="NTLTGASLLTNSVETFNETSTLEIKKWYSYATVPLASVITGNLG
                     NVTISTQVGSTLFEWNQNNQFESHQIKGTLTINNGWVVLDKSGSISDTKIGKIILPNV
                     NSVLEFHSGSHPASFKVTTDSIANIGGEITGLLNGEGNVTLNVNGGFLNMGNVELIYN
                     KGTLNTANGNAKLNVGGKYLQTHGDLRGVFNLSSTAAGNTEFKFNSLEMTGGLLFAYY
                     ACNATGALNSVTVTNDMVLNFSNASDKFRVNGLTTLAGQYSKSRSLLKVDGNLILSGN
                     AAAEFTSSGSVGAEENRIKGDVNISGLNSNFNMGSHAVILSVNGDVNIDGGNTCFSKT
                     PGVSQISLNKNFSLQSGTFILKGHEGKSELTVNGAYSQTGGVFYLHSNTLTASNDSVK
                     AYFKSSFRQWNGIINFDDNPEGGKSVLFIQGNEFIAKGSSLITRAGTGIGSVFGNLIY
                     CTNGDLNYQRSTASVIDQIKQRITVNCRLKVTDGNLLLSSHSQPAVDFLRVESGAILN
                     LLSTQIKSNLKYQNSGFTVDAGGLVRTANSKGFYNTLGTAAVCSNGNLNYFLDRNSIV
                     DYVGSNQLITGSGSGVINKEQFKYGVLRVSGNGILATNVEVRNRIELGSSTIKLNQKT
                     LIIENENTNALSASGGSLICASESDKLIWKNMKGGSSYTIPFSSDGKVVSNVVVQPIV
                     SGDISMYNYTGNPVSENLPDGIQPFLVDGKDVTDESLARRIIGFDSQVKANITLKFFK
                     NEIPGFKNGDTLVLIKNIGQWEVTPFSKVGDEQNPVSVQATNINANAVFALCTGIGAK
                     MPDNTNGNNNSQGNFSVEQISPVPFNDFLKVNINAPTIGVIYLTIVGSDGRELRKQMF
                     DVSPGKNLLALSELSSLPTGVYFIQINGFKQTITKKVMRQ"
     assembly_gap    10860..10904
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10905..11272)
                     /locus_tag="PK134_01965"
     CDS             complement(<10905..11272)
                     /locus_tag="PK134_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37650.1"
                     /translation="MSRIRIQFLVFSLIYFFAYCNAVFAVTKTTTASGSWSNPAIWSP
                     TGVPASSDDVVISSAHNITISQQTFCKTLVVSGGAVLSLSPSKLLQINGAVNIAGTFD
                     MNGGNVKQLLNNAALQIGVTG"
     gene            complement(11259..11552)
                     /locus_tag="PK134_01970"
     CDS             complement(11259..11552)
                     /locus_tag="PK134_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37651.1"
                     /translation="MRKKRKKHLPNRLRQSAKLRLSYRLIAVTATVTMAVVVGGIIYF
                     NISNSKKSKASTTTSDAEQRPVDFNIPDLKQNFREETINGIRVRKMRDEHVTN"
     gene            complement(11644..>12845)
                     /locus_tag="PK134_01975"
     CDS             complement(11644..>12845)
                     /locus_tag="PK134_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB37652.1"
                     /translation="KKCRSINNKTMAKIFIVEDDAMVATLIRQSLSKNPDFEIQHFES
                     AEECLNNLHLNPDIVTIDYHLPGMNGVGLMRRIKDYNASIMCILVSGQDSLEVVVDSY
                     KSGAQDYIIKNDNLFVNLENSVKNLSMNVVLKKENEMLKDQIIDRHKYSNIMGSSNAV
                     LRILRLIQKVEKSNMMVLVTGQSGTGKELVARALHYNSPRARKNFVTVNMSAIPEELI
                     ESELFGHEKGAFTDARERRIGKFEEANEGTIFLDEIGEMPLSLQAKLLRVLQEKEIQR
                     IGSNKTIKLDFRMVAATNRNLAVEVKEGRFREDLFYRIQGFLIHLPPLKERGDDIILL
                     AKHFLDLYAQANKTPVAQLSNEAAKFMLDYEWPGNIRELKAVMERAAILADNATISVD
                     DLMFVNL"
BASE COUNT         4059 a         2430 c         2107 g         4178 t
ORIGIN      
        1 cttgttccta aacaaattga tggcacagta tctaaacgac tttttacacc tgccctatgt
       61 gcacactcaa caattttgtc ccatccaacc aattcagcaa tttgtgctgt aatagaattg
      121 accgattgtg ccaacgcccg tcttaatgtt ttacttgaat aagtaaatgc acctgtacta
      181 ttatagggtt gccagatttg accaccatca tactctatgg atacaggcac atcatctaat
      241 cggctacaag ggctaaaagt atcttctatt gctgccaaat aagcaaacgg cttaaatgtt
      301 gaacccggct gtcgttttga ttgtgtaaca tgatcgaact taaaaaaatt aaaatcaaca
      361 ccacctacat aagctttaat ctcaccggtt aacggattca aagacatagt tcctgcattt
      421 aacaaacgtg cataatattt aagtgaatct aatgttgaca tcacggtatc cttggcacca
      481 ttgtatgtaa atactttcat gctttttttc ttgttaagat aaaaattaac agaatctggt
      541 gctgaaggga attgcttttt caaacggtcg tatgactgcg taaatgccac agcggtattt
      601 gcgaagttca atatttctct tccattgtca tcgcgccatg gattttgttt tccccaatgt
      661 tcatcgaatt gcttctgcac ttttctcaga tgatctttaa cagccaactc tgcataattt
      721 tgtaggtgtg agttaatggt tgtataaatt ttcaatccat cctcattaat gtttaagttg
      781 ttttcatcac aataactttt aataatcttt gctattaact gcctcaggta agaatctttt
      841 gttgcagggt tattggttgg acgtttaagg ttcaatggca ttggactgat ggaatcaaaa
      901 gctgcctgac tgatataccc atacttctgc atctgcgaca acacaatatt tcttcttgcc
      961 aatgagcgtt tcttattatt caaaggatta taactacttg tagctttgag caaaccaatg
     1021 agtgttgctg attcctgcaa atttagttct gaaggttgtt tgctgaaata gttttgtgca
     1081 gcaaccttga taccaaacca attattacca aaatcaactg tattaaaata catttccaat
     1141 atctcatcct ttgaataaaa catctctaat ttaacagatg ttatccactc ttttgatttg
     1201 ctaaccaatg taccaacaag aggaatatgt tgaagtaatc cctgattaac tttcttgcgg
     1261 gtactgtaca tatttttggc tagctgttgt gttattgtgc tagcaccacg ctcatctcct
     1321 ttggcagtgg aatatgcacc actgattaat gcataaaggt caaggccgaa gtgtttatag
     1381 aaccttacat cttctgtggc aacaagtgcc tgtactgcat aagtcgaaat tgaatcaaaa
     1441 cttacaggtg tcctgttttc atgataatat tttccaataa gtacactatc ttctgtataa
     1501 atttcggtgg caattgccac ttgcggattt ctgacttcac tcatttttgg catatagcca
     1561 aatagccaaa gaaagtttat atgcagtagc acaacataat aaaccatcag cataaacaaa
     1621 atagctgcac ttcttaaaaa cttgttcttt attattttga gataggaaaa cgttataacc
     1681 gattttaaaa tcagaaatag ctgtttataa attctttgaa aagctgaaac accctcaggc
     1741 atattttgtg gcattaagtc aaacacatta acaagttgca aagaaataaa cctttctgtt
     1801 taatttcatc ttttaggcat acatttgtca gcagtttttt aactaaacat aaatgagctt
     1861 aacagaacag tttaaagaag tcgtaaataa agaatatcaa tttaaaggcg acagcatcgt
     1921 actcggtgca ccggtactta aaggcgaaac gctgacagga agtttcataa aagcaccatt
     1981 aaaaactttt aaccgtcatg gtttaattgc aggagctaca ggaacgggaa aaaccaaaac
     2041 catgcagggc atagcagaat cgctttctgc gaaaggtgtt tcatgtttat tgatggatgt
     2101 aaaaggcgat ttaagtggat tggcaagtcc gggaacaaca aatccaaaaa ttgatgaacg
     2161 ctatcagaaa ttaggactta cctggaagcc actgcaattt ccggttgagt ttctgacttt
     2221 atcnnnnnnn nnnnnnnnna aaaaggtgtg cgattaagag ctactgtgtc tgaatttggg
     2281 cctgtacttt tttctaaaat ccttgatttg aatgacacac aacaaggtgt tgtttcgctg
     2341 atatttaaat attgcgatga taaacaaatg cctttacttg atttgaaaga tttcaaaaag
     2401 gtattacagt ttttaactaa tgaaggtaag gatgaaatta aagcagatta tggtgctata
     2461 agtagtgctt cggcatcaac catcattcgt aaagtaatag agatagaaca gcaaggtgca
     2521 gagaaattct tcggtgaaaa atcttttgaa gtcgaagact tgttaggaat agacgaaaat
     2581 ggcaatggca aattgaatat catcaggttg accgatattc aggataagcc taaattattt
     2641 tcaactttta tgttgagttt attggctgaa gtatattcaa catttcctga agtgggtgac
     2701 ccggatgcac ctaaattggt aatatttatt gacgaagcgc atttaatttt taacaatgca
     2761 tctaaagtgt tgttagatca gattgaaaca gtaataaaat tgattagatc aaaaggcatt
     2821 ggagtatttt ttgttacaca atcaccggaa gatataccgg ctgccgtatt ggcgcagtta
     2881 ggattaaaag tacaacatgc tttcagagta tttacagcaa acgacagaaa agcattaaaa
     2941 cttatagcag aaaattatcc gctatcagac tactataaag ttgatgaatt acttactact
     3001 ttaggtattg gtgaagcagc aatcacagtt ttaaatgaaa aaggtgtgcc aacaccaata
     3061 gctcatactt tgctttgtac accggcatca cgaatggaca ttttaactcc ggatgaattg
     3121 acaaaaagta tttctaaatc tcgtctgaca acaaaataca atgaaacact tgacagagaa
     3181 agtgcatttg aagtactctc taaaaaatta gaaacgcagg ttgctgaaaa tacaaaagca
     3241 acaaaagcag ctgaagcaaa agaagaaccc agccagttgg atcaggtgct aaacagttct
     3301 acaggaaagc aagtgatgag aactgtaaca agtgtactaa caagaagctt acttggagca
     3361 ttaggcttag gtgcaacaag acgcaaaaag tcgggttggc tctgatattg catttattaa
     3421 gttatgacat cacctaagtc agagtggaag tatgatttaa tatcaaccat atttccaagt
     3481 aagtttttaa agcgcgaaat acgaattgat tattatttgc ctgcacaata ctctaaaaac
     3541 accggagtat tgtttatgct tgatggtcag gatgcagaat tgttacacct aaaagataca
     3601 ctgcagagtt tgacacagtt aaaaaaaatt aaacctatca tagtaattgc tattcatgca
     3661 aataaaaaca gattgcagga atatggtgtt gtaggtaagg ctgactatct aaagcgtgga
     3721 aataaagcaa aacaattttc gaaatttatt cttgacgaac tgattgcgca ttgcagcagt
     3781 tttacaggaa taaaatttac accatccaaa actgcaatag ccggtttttc attgggtgga
     3841 ttaacagctt ttgatttggc atggaaccat ccttctgttt ttgaaactgt aggtgttttt
     3901 agcggcagtt tctggtggag aaaaaaatct tacgaagacg gatatactga tgatgacaga
     3961 attatgcatc aggtggtaaa gaaaagtaaa tcggcaccgg caataaaatg ctggattcaa
     4021 acaggcacta aagatgaaac tgcagacagg aataaaaatg gtgttattga ctctattgat
     4081 gatgcccgtg atctgatttt tgaaatgaaa caaaaaggtg ttcctgaaaa aaatattcaa
     4141 tatgtagaaa ttcccgaagg gcagcataat cagataacat ggtctgcagc cctaccctta
     4201 tttttgtgtt gggcatttgg caacaaaaga aaagttgata tttattgatt atactttttg
     4261 atgtgaggtg tttaagcttt cagcaaaaga ttttagattt aatccgtcaa aagtacctga
     4321 gctcatcatt aaataaacta cttgttgttt atcttctgtg ttcagaaaat ttattaattg
     4381 cattgagtca ttaataactt ttatgtcgct tctgtcaaat gattggaaaa ttttttcgga
     4441 actaatggga ggcaattttt tatgttcaat agtttttgga ttaaaataaa ctactgcaac
     4501 atctgcatca gccattgtgc ctttatattc atcaagaaaa ttttcattta aactgctaaa
     4561 cgtatgtaac tccatacagg caataagttt tcgttccggg aattgcctgc gtaccgcttc
     4621 taatgttgct ttaagttttg aaggtgaatg tgcaaaatct ctaaaaacgg caaactgatc
     4681 attctcttca agtaattcaa gccgtctggc tgctccttta aatgatttta ttgcattata
     4741 aaaaacttcg ttattgacac ctattgagtt gcatatcatc cttgcccctt ctagattcat
     4801 cagattatgc tcaccaaaaa tctgaagcgg cacttcctta ttatagtcat cgtaaataaa
     4861 tgtttttcca tttctaataa catgtttcgg aatggaataa ccttttctat cagctacaac
     4921 attttcagcc tcacaaattt tacgaacagc atcatcattc atgcagtaaa acaattttcc
     4981 atcacgtgga atttctcttg caaaaatcct gaattgctcc tcataaatct ccagagttgg
     5041 aaacacattt atatgatccc atgcaatgcc actaatgaga ccaatttgtg catgatacag
     5101 atgaaactta ggtcgtctgt caatcggaga agacaaatat tcatctccct ccagaacaat
     5161 tataggggca tcgtttgata gtttaaccat ggtatcaaaa ccttcaagtt gtgcacctac
     5221 cataaaatca aagttatttc ctgatgtttt taaaacatgt agaatcatgg atgtaatggt
     5281 tgtcttgcca tggctgccac ctattacaac tctggtttta ttttttgaat gttcatacaa
     5341 aaattcagga taagaaaaca ctttaatatt tttttgaata gcctctgcaa gttcagggtt
     5401 atcggcacga gcatgcatgc ccaatataac ggcatcaata tcagcattaa ttttttcagg
     5461 aaaccaacct gttttctcag gtaatattcc cgatttagcc aatcttgtct ttgcaggctc
     5521 aacaatttca tcatcactgc ccgacacttc atgaccaagt tgatgtaagg caagtgccag
     5581 attatgcata gcacttcctg caatagcaat aaaatgatat ctcatttccg atttttgacg
     5641 ctaaaatacg ctgtcataat ggggtatttt taaagcttca aactttttat taacgatatt
     5701 catccctgtt cttgttatta aagataagtg atttaattta gttttgcgca aaaaagtttg
     5761 cattgaaggc aaaaatctta gacctctaca ttataaaaaa gtttttaggc acattcctgt
     5821 ttgccatagg actaattatt tgtattgccg ttgtgtttga tatttctgaa aaagtagatg
     5881 attttattga gagacatgct ccattaaagg ctattgcatt cgattattac ctaaacttca
     5941 tcccctattt tgccaattta ttcagtccat tatttgtttt tatttcagta atatttttca
     6001 cctctaaaat ggccgggcag acagaaatta ttgccatttt aaacagtggc atcagtttca
     6061 accgctttct ccgaccttat attatttctg caacagtaat tgctattgca tctttttatt
     6121 taaacaactg ggtgattcca catgcaaacc ttgtacggct tcaattcgaa aatatttata
     6181 ttaaaaatcc ttatgtctat cgcggaagaa atattcacag acaaataaaa cccggtgagt
     6241 ttgtttattt tgaaagctac gataacaaag ccaacacagg ctttatgttc agcattgaaa
     6301 aatttgaagg aggaaaatta gtaactaaac tcatggctga tagaattaca tgggattcat
     6361 taacgcaaaa gtggagcatt gaaaattata ccatcagaca tattaatggt atgacagaag
     6421 aattaaagaa aggcatcagg ctagatacta cattatcaat acacccatct gaatttaaca
     6481 gaagaatgaa ctttattgag gcaatggatt tttcggagtt aaacagctat attaaagaag
     6541 aaaaatcaan nnnnnnnnnc ttgaaaactc agtaaaaaat caagaggctc tgaagtgatt
     6601 cctttttacg aagtagaaaa atacaggcgc acctcctatc cttttgcaac tttcattctt
     6661 acacttattg gcgttgccat ttcaagtaaa aaagtacgtg gcggtatagg tttacaatta
     6721 ggggcaggaa tattattgag tttcacttac attatgttta tgcaggtgag cacaacattt
     6781 gctacaaatg gaaatctgcc ggcagttgtc gctgtgtgga tccccaattt agtatatgca
     6841 tttattgcca tttacttatt acgaaaagca cctaaataaa aagaggctgt cattaatctg
     6901 acagcctctt ctattaaacg gatcaaattt tatttcaccg tgtcagctgg ataatcagcc
     6961 ggattaaatg ctgtttccgg gtggttcatc ctataacgat accctataat cggcaccgct
     7021 attgatttta atggtttgtt tgaaggcatc aacctgtaat aggcaatttt gttttctaaa
     7081 ggagctttat ctacgaatgc ataagcacgc aacacttcag aatttccatt agcttttata
     7141 gctcctattt tttgatattc aatagcatct acagagcgct caacaacaaa ggaatctata
     7201 tctgtttcgc gagaggtgta ccaactaacc attacatgat gatcgcgagc ctctgcttgg
     7261 aaagaaagta atgtgattgg cagttgttgt acatgcgttg tgctacgaat aataaaatta
     7321 tcaagaatag ttttatctgt accgccaccg ttcatattct ttgcaataac aatattgtca
     7381 gaatttttcc agaacattga tcgctctttt ggcccctgat aattccaaac cactacatca
     7441 tcaaccataa tttcaccttt acctgtattt ggattataga caaagcggta atttctgaaa
     7501 tcattatctt ttaccaactc ataatttgtt gtttcattaa cagaatagct tcttcctttt
     7561 tcgagattta cacgataggc aataatcaat tttccgttct tcattccaaa gttgaaataa
     7621 cgaccacgac ttataaaatc gcaattatca tctagtcttt tataatcaaa gcttatatca
     7681 attccatcag tattaaaata attatctgtt ggaatagtca aatcaattcc atctttacct
     7741 ttacctgcgc tcaatccgtt tgtgccttca acaccttctg acattactac agcaaaaggg
     7801 cttactttta ttgcatcagg acctttactt gcctttgtag gatcgtcacc attccaatca
     7861 aatgcacaaa taacttctcc gttatttaca tcatacccct gaatcatatt ggtgagtgct
     7921 ccagcacgtg aatctgtcac attggaagta ttcataacga cagtaagata tactgccaat
     7981 cccattatag atgttccggc agtaacaaaa attgccttat acattataga catcttttgt
     8041 cgctgacgga gttttaattt tcttgccata ataattattt tatagaatga accttttgat
     8101 tatactctta gagattaaaa aaggttgtaa aaacttaaat cgaaatttgt gatttcttac
     8161 atttcattac tgccgcatta ctttcttggt aatggtttgc ttaaatccat ttatctgtat
     8221 aaaatagaca cctgtgggca aactactcag ttcacttagt gctaacaagt tctttccagg
     8281 agaaacatca aacatttgtt ttctaagttc acgcccatca gagcctacaa tagtcagata
     8341 aattacccca attgttggag cattaatgtt tactttaaga aaatcattga atggtaccgg
     8401 tgaaatctgc tctacactaa aatttccctg agaattattg ttgccattag tattatcggg
     8461 catcttcgct cctataccgg tgcaaagtgc aaatactgcg tttgcattta tatttgttgc
     8521 ctgaacagaa actggatttt gttcatcacc tactttactg aatggtgtca cttcccattg
     8581 tccaatattt tttatcagaa ctaatgtatc accatttttg aagccaggaa tttcattctt
     8641 aaagaacttt aaagtaatat ttgcttttac ttgtgaatcg aaaccaataa ttcgtcttgc
     8701 taaagattca tcggttacat ccttaccatc aacaagaaac ggttgtatgc catcaggtaa
     8761 attttcagaa accggatttc cagtatagtt atacattgaa atatcgccag aaacaatcgg
     8821 ttgcactaca acatttgaaa caactttacc atcacttgag aaaggaattg tataactact
     8881 tccacctttc atatttttcc aaataagttt atctgattct gaagcacaaa tcaacgagcc
     8941 gcctgatgct gataatgcat tagtgttttc attctctata atcaaagttt tttgattgag
     9001 cttaattgtt gagctgccta attctatacg attgcgaact tcaacattag ttgccagaat
     9061 gccatttcca ctcaccctta aaacaccata cttaaactgt tctttattaa taacaccgga
     9121 gcctgaaccg gttattaatt ggttactccc tacataatca actatactat ttctgtcaag
     9181 aaaataattc aggttaccat tactgcaaac tgcggcagtg cccaacgtgt tataaaagcc
     9241 ttttgaatta gctgttctta ccaaacctcc cgcatcaaca gtaaaacctg aattctgata
     9301 tttcaggttc gacttaattt gtgtacttag taaatttaat attgcacccg attcaactct
     9361 taaaaagtca acagcaggct gactatgact actaagtagt agattcccgt ctgttacttt
     9421 aagtctgcaa tttactgtaa tacgttgttt aatctgatca attacagacg ctgtgcttct
     9481 ctggtaattt aggtctccat ttgtacaata tatcaaattt ccaaaaacag acccaatgcc
     9541 tgttcctgct cttgttatca atgaactgcc tttggctata aattcattcc cctgaataaa
     9601 aagtacagac ttaccgcctt caggattatc atcaaagttt ataatgccat tccactgacg
     9661 gaatgaactt ttaaagtaag cttttacaga atcatttgat gcagttaatg tattcgagtg
     9721 caaataaaat acaccaccgg tctgcgaata tgcaccgttc acagttagtt ctgacttgcc
     9781 ttcatgaccc tttaagataa aagtgcctga ttgtaatgag aaattcttat ttaaactaat
     9841 ttgtgaaact ccaggtgttt ttgaaaaaca ggtgtttcca ccatcaatgt taacatcacc
     9901 atttacagat aaaataacag catggctgcc catgttgaaa ttactgttaa gccctgatat
     9961 attaacatcc cctttaattc tgttttcttc tgctccaaca cttccacttg aagtaaattc
    10021 tgctgcagca tttcctgaaa gaatcaggtt gccatcaacc tttaacaatg agcgcgactt
    10081 gctatactga cctgctaatg ttgtaagtcc atttactctg aatttatctg atgcatttga
    10141 aaagttaagt accatatcat ttgtaacagt gacagaattt aatgcaccag ttgcattaca
    10201 agcataataa gcaaacaaca acccacctgt catttcaaga gaattaaatt taaactctgt
    10261 atttcctgct gcagttgaag acaaattaaa aacacctctt aagtcaccat gcgtttgcag
    10321 gtacttgccg ccaacattta gttttgcatt accattagct gtatttaaag ttcctttatt
    10381 gtagattaat tctacatttc ccatatttag aaagccaccg tttacattca gtgtaacatt
    10441 gccttcacca ttgagaagac cagttatttc accaccaata tttgcaatac tatctgtagt
    10501 tactttgaaa gatgctggat ggctaccgct atgaaactca agtacagaat ttacattggg
    10561 taaaataatc ttaccaattt tagtatcaga aatactacct gatttatcca atacaaccca
    10621 gccgttgtta atagttaatg tgcctttaat ctgatgactt tcgaattgat tattctgatt
    10681 ccattcaaac aaggtacttc ccacctgtgt gcttatagtg acatttccta aattaccagt
    10741 aatcacagac gccaatggta cagttgcata agaataccat ttcttgattt ctaaggttga
    10801 tgtttcatta aaggtttcaa cagaatttgt tagtagcgat gctcctgtta aagtgttatn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgta acaccaattt
    10921 gtaatgctgc attattaaga agttgcttaa cattaccacc attcatatca aatgtacctg
    10981 caatattgac agctccattt atctgaagta atttagatgg tgacaaacta agaacagcac
    11041 ctccagaaac aacaagtgtt ttacagaatg tttgttgtga tattgttata ttatgtgcag
    11101 atgaaataac aacatcatca gaagaagccg gaacaccggt aggtgaccaa atagcaggat
    11161 tactccagct accggaagct gttgtagttt ttgtaacagc aaatactgca ttgcaataag
    11221 caaaaaaata aatcaatgaa aagacaagaa attgtattct aattcgtgac atgctcatcc
    11281 ctcatctttc ttactcttat accattaata gtttcctctc tgaaattttg tttcaaatca
    11341 gggatattaa aatccactgg tctttgctct gcatctgatg ttgtagtact tgcctttgat
    11401 tttttggagt tggagatatt aaaataaatt attccgccaa caacaacagc catagtaact
    11461 gttgcagtaa cagcaattaa acgataagac agtcgtagct tagcgctttg tcttaacctg
    11521 ttgggcaggt gtttctttct tttcttcctc atctgcgtaa atccttatat gtaaaatcgc
    11581 taaaaaggtt gcataaaaaa agccgcatta cggtgcggcc ttataatatc actcagtact
    11641 aatttataaa tttacaaaca ttaaatcatc tacagaaata gtagcattat ctgcaagaat
    11701 ggcggctctt tccatcacgg ctttaagctc acgaatattt ccgggccact cataatcaag
    11761 cataaattta gctgcttcgt ttgagagttg tgccacaggt gttttatttg cctgagcata
    11821 caggtcaagg aaatgttttg ccaaaagaat aatgtcatca cctctttctt tcaatggagg
    11881 caaatgaatc agaaatccct gaatcctgta gaataaatct tctctgaatc ttccttcttt
    11941 aacttcaaca gccagatttc tgtttgtggc tgccaccatt ctgaaatcga gcttaattgt
    12001 tttattactt cctatacgct gaatttcttt ttcctgcaat acacgaagca atttagcttg
    12061 taagcttaaa ggcatctctc ctatctcatc aagaaaaata gttccttcat tagcttcttc
    12121 aaactttcct attctacgtt cgcgggcatc tgtaaatgca cccttttcat gaccgaaaag
    12181 ttcactttca ataagttcct caggaattgc actcatgtta acggtaacaa aatttttgcg
    12241 tgcacgaggc gagttatagt gtagcgcacg tgcaacaagt tctttacctg ttccgctttg
    12301 tccggtaaca agcaccatca tattgctctt ttcaactttc tggatcagac gcaaaattct
    12361 taacacagca ttactgctac ccattatatt gctgtattta tgacggtcaa taatctggtc
    12421 ttttagcatt tcattctcct tcttcaacac cacattcatg ctcagatttt ttactgagtt
    12481 ttcaagattc acgaacaaat tatcattctt gataatgtag tcctgtgctc cgcttttata
    12541 agaatcaaca accacttcaa ggctgtcttg tcctgaaaca agtatgcaca tgatggatgc
    12601 attataatct ttaattcttc gcatcagacc tacaccgttc attcccggca agtgatagtc
    12661 aatagttaca atgtcagggt taagatgcag attatttaaa cattcctctg ccgattcaaa
    12721 atgctgaatc tcgaaatctg gattttttga caatgactgg cgtatcagtg ttgccaccat
    12781 tgcatcatct tccacaataa aaattttagc catagtttta ttatttatac ttctacattt
    12841 tttag
//
LOCUS       DAORQT010000026        12492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_550_length_12492_cov_9.824315, whole genome shotgun sequence.
ACCESSION   DAORQT010000026 DAORQT010000000
VERSION     DAORQT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_12492_cov_9.824315"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>278
                     /locus_tag="PK134_01980"
     CDS             <1..>278
                     /locus_tag="PK134_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRB37653.1"
                     /translation="LTKLNGFSGQVNIWKVHGSLDWFKSKEEVDFHLPLRQTIPTDFK
                     PSIVTPGLSKYLETQLEPFRTIFTEADSEIEKAVSYLCIGYGFNDIHV"
     assembly_gap    279..319
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <320..520
                     /locus_tag="PK134_01985"
     CDS             <320..520
                     /locus_tag="PK134_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRB37654.1"
                     /translation="PIIVITKELTPKTKESIIDSKCKQYILIEEANKKDTRIYSSSFA
                     GEQIVPDVSYWTLGEYLKLIKS"
     gene            524..2275
                     /locus_tag="PK134_01990"
     CDS             524..2275
                     /locus_tag="PK134_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB37655.1"
                     /translation="MAIFNFQNDAAIGTVFSVDTATVIIKVDEIDKLRKLHVNHLVAI
                     KSSKAGQHLIGIINRITRKASDEEKPTEEDLGLGGFLFTENILKVNLIGTLHDKHGQK
                     ENVFKRNLDTVPEIEAQCFPIDGENITKFMTTISSVDNYKVPLTIGKYSIDEHAQAFL
                     DGDKLFQRHAVIVGSTGSGKSWCVAKFLEQIAQLPMADCILFDIHGEYSGKDFKMDGV
                     QHLRVANPSDLGKKGNLANNVLMLPYWMLTYEEMLAMLLDRSDSNAPNQAMLFSKTVF
                     TEKQKFLDAIKDKIFKDHITIDSPIPYKMENVLDELQRMDEEMVAGAKVGTEKQGPFH
                     GKLTRFIQRLEAKSQDKRLGFLFQLSEDELQIDWMNTLCERLMFGSALSKEKSGVKII
                     DFSEVPSDVLPLVIGLVARVVFTVQQWTAKENQHPISLLCDEAHLYIPERTNQDAAAE
                     LGLKSFERIAKEGRKYGVSLTVISQRPAEVNRTVLSQCNNFIALRLSNAEDQAVIKKL
                     LPDNLSGLTDVLPILDIGEALVVGDASLLPTRVIIDEPKIKPESATIKFWQEWSNDKA
                     KQDIATAVTGLRKQSKA"
     gene            2291..2950
                     /locus_tag="PK134_01995"
     CDS             2291..2950
                     /locus_tag="PK134_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRB37656.1"
                     /translation="MAEEYLDIYNSKGVHLGKELRKVVHQKGMWHKTFHCWVIFRNEA
                     GEDCILMQKRAAKKESAPNKLDSAVAGHYTAGEDISGGIREFKEETGIDIDSKDLIPL
                     GIRVTVSDYKPNSINKEFQEVYFIKKSIPLESFKLPVDEVAGMVELPVKKCIALLDKQ
                     IDHFFARGIFNDDASSPNIAIIIERKITIDDFIWFIDNFHFKIMLLTSKALANEKYLF
                     I"
     gene            2950..4191
                     /locus_tag="PK134_02000"
     CDS             2950..4191
                     /locus_tag="PK134_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron reductase"
                     /protein_id="HRB37657.1"
                     /translation="MLLENLSIEKLPYLERYVNSGSPSGFTNRYTTSEETKPFGLSPF
                     FHPLICVAPEECFKNYGKIPAFKEFEEVTGKNWLLVHPDMKKEKVFKAKNVSLTKSKK
                     IKLVPTASGRTVELFNSSTKGYFKLHYTHVIGRITRELDNKIGVAGPEISKHLVSLID
                     QNKLDSRLTLMRETGARIFQGHVNEKPVEWGMVWREKDAYKIDYNEVKYVIPVFSLFG
                     KDILAKRDKPLLQQIINLIGANPTDYVLKQLIFPMIELYFNLIKNSGIQPEWHAQNLL
                     IGFTKDFAPVKFIMRDLESMDKDLTFMEEHNIKAKFESYPFKCIFREQWNYQRKHSFM
                     YDYKFGEYIIRPLLDFLKKKYKVDTLPLHQKIKLHSKKFINEFSKDFFPEYWVVFRKI
                     LINQKTDKRPYVHRKNPKFRY"
     gene            4192..4860
                     /locus_tag="PK134_02005"
     CDS             4192..4860
                     /locus_tag="PK134_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRB37658.1"
                     /translation="MHIVFIRHTESEKNVNNQFSSYKDKEPLTEIGMSQSENLGNDLL
                     EFKERKNLTCKNVYAASSTRAIETAKIIASKLNATVSVEDALRSTKPGALSGKSEEEG
                     LKTNPDFLEQLYLFRNGLFNAYDFTVAEGKEPKKDFEKRVWGRLSEIISDKSESLKII
                     SAHRSSITYMLLEFAKRYYKYPQNMSGHVLLDLGKISLVETVAENKWKITRVNFDSSE
                     LKTL"
     gene            4864..5943
                     /locus_tag="PK134_02010"
     CDS             4864..5943
                     /locus_tag="PK134_02010"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37659.1"
                     /translation="MSENKTNKHSYLSALIQLGKFAWGIFIGISILVTVATMVTDFKM
                     TKEHPVLTGLAVLAVTIFISLILLVIKMINEGVEQKSPLPLSTELVNIINTLYEKEKY
                     LDVVRLGSALSRYLWLKGKYHERIEIGERVEDAAGKESRIEEQVSSLIDDIGWTYNII
                     GQPDKAVENITSGIDKALTNELFYYAAKGERHLSGIEKHKGHQAECLSHLQKAKEHSE
                     KILDLTSKNEMEASLFLAEAKYLFELGKLNEAEALAEKAIAVFKNDPDRIVKIHSLLG
                     NIYLKQNKLQKAKDEFNKGYNATKSTREDEVAKNLKGLADIALLERDIKSGLKYLNEA
                     KEIYLSIHKNKEATDLDNIIRSIRI"
     gene            5964..6593
                     /locus_tag="PK134_02015"
     CDS             5964..6593
                     /locus_tag="PK134_02015"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRB37660.1"
                     /translation="MPSLILTSDDFGLSKIFNQRMIELLQSNFLSSISIMINQVTSDQ
                     QNQIAEIKKMYAKNNFSLGLHLEVSEADDEATYQSQWNMFEQLLSIPPDYIDVHKGHF
                     QNATFNSIAEFCLQKRVRFRKYKETTVNVSSPESSLTATFKELGAIKEWIANFKTNST
                     YELVFHIGSFDPDSKSSLNKDRELDIEKLKHVHEFISDKNISITNYKSL"
     gene            complement(7311..7721)
                     /gene="arfB"
                     /locus_tag="PK134_02020"
     CDS             complement(7311..7721)
                     /gene="arfB"
                     /locus_tag="PK134_02020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRB37661.1"
                     /translation="MGKIDFKCLFSEVILTYSRSSGKGGQNVNKVSTKAELAFDIENS
                     VCLDEEQKKVITEKLCRRINEDGFLKLQSSEARSQLGNKELVLKKFQMLIEKAMHRPA
                     KRISTKIPAQVKEERLTQKRRTAEIKKLRQKNVD"
     gene            complement(7721..8503)
                     /locus_tag="PK134_02025"
     CDS             complement(7721..8503)
                     /locus_tag="PK134_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRB37662.1"
                     /translation="MSTLRVTTVQTKLHWESQAKNIKHFTTLLKSIKAGSTDLIVLPE
                     MFSTGFSMNASVLAEKNDGPTMQWMQKMAETKNAVVCGSLIVEEGGRYYNRLVWMTPG
                     GAHAHYDKRHLFTMAGEQKFYHPGNKKLILLYKGWRLSVFICYDLRFPVWMRNQNEYD
                     CAIVVANWPEKRSFAWKQLLTARAIENQSFVVGVNRIGKDGNDITYSGDTVVLDPFGK
                     KISKTKSATENIETVKLDFDVLENARHIFPVGNDADSFTIKL"
     gene            complement(8558..9721)
                     /locus_tag="PK134_02030"
     CDS             complement(8558..9721)
                     /locus_tag="PK134_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRB37663.1"
                     /translation="MSPNQALIKSKLPKTGNSIFSVMSALANECNAINLSQGFPDFAI
                     SPELISLVNKYMKKGMNQYAPMPGIMPLREEIAAKIEARFSAKYNPETEITITAGGTQ
                     AIYTAIASVVREGDEVIIFDPAYDSYAPAVVLAGGEPVHAELKMPDFHIDWNEVKKLM
                     NQRTRMIMINTPHNPTGAILSVKDFQMLEKITAKTDIFVLSDEVYEHVIFDGYEHQSV
                     ARFPKLAERSFIVYSFGKTFHTTGWKMGYCIAPAKLSAEFRKAHQFMVFSANTPIQYA
                     YAEFMKDAKNYSGIEKFYQEKRDNFLKLIQGTAFKPLACSGSYFQLLDYSKLSNEKDM
                     DYAVKLTKDYGVASIPVSAFYRNGTDHKLLRFCFAKSEETLEKAAERLHKVEK"
     gene            9982..11736
                     /locus_tag="PK134_02035"
     CDS             9982..11736
                     /locus_tag="PK134_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRB37664.1"
                     /translation="MKFPSINFSVYGKRTYLVLVSVLIGALTTLCAALLKKGVMLMEH
                     IAEHLVFDSGYHQLFLILPAIGILLTVTFWRLMKIKNERRGMTAVIYNISKQSSVVAR
                     HKLFSQMVSSIFTVGFGGSAGLEAPIASTGSAIGSNIASKLKFSTKERTLLLACGAAA
                     GISAIFNAPIAGVVFALELLLIDMPVPVVIPLLISSATDDFLSRYFNLVQPFVLITDT
                     WNSSHILYYLALAALAAIISIYCIKIYFFISSLFSKITNVFVKALIGGTLLGLLIFLF
                     PPLYGEGYDSVQLLLHNQSAEIFKNITFFEANALWLPILAALLMFFLKVVATSFTVFG
                     GGNGGMFGSSLFTGAMLGFTFSRVVNIMGWAELNEINFTVIGMASMLSGVLQTPLTAI
                     FLIAEITGGYALFVPLMITTSITYLATRYVEPHSVYTKQLALKGGLINLSKDKQVISK
                     MKLRDYLETDLETININATLGDLIKAIRKSRRNIFPVVDAMNHLQGIILLDDVKSIMF
                     DQSQYDKGVKELMQRPPLVLDIDEDMERVLEKFEEYEAWNFPVTEKGVYAGFVSKSKI
                     FTFYRQLLIQESDVPMHD"
     gene            complement(11796..12086)
                     /locus_tag="PK134_02040"
     CDS             complement(11796..12086)
                     /locus_tag="PK134_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37665.1"
                     /translation="MNNSMIESTTGAEMVEAKKAAPKKKAAKKKAAPKKKATKKKAAT
                     KKKVVSAKKAAPKKKAATKKKAAPKKKAATKKKAAPKKKAATKKKAAAKKKK"
     gene            complement(12154..>12492)
                     /locus_tag="PK134_02045"
     CDS             complement(12154..>12492)
                     /locus_tag="PK134_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRB37666.1"
                     /translation="KNNLWKIVNALQNGLKQAGFDIGKTQSPVTPVYLNGTIPEATKL
                     IYDLRENYDIFCSMVVYPVVPKGIIMLRLIPTAVHTLEDVKYTIESFGKIKSKLLNGE
                     YKAEKIVDVN"
BASE COUNT         4052 a         2150 c         2371 g         3878 t
ORIGIN      
        1 atttgaccaa gttgaacggc tttagcggac aagttaacat ctggaaagtt cacggctcat
       61 tggactggtt caaatcaaaa gaggaagttg attttcattt gccattgcga caaactattc
      121 caactgactt caaaccatct atcgtaacac ctgggctttc caaatatttg gaaacgcaat
      181 tagaaccgtt cagaacaata tttacagaag cggacagtga aattgaaaag gcagtttctt
      241 atttgtgtat tggttatggt ttcaatgaca ttcatgttnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnc caattattgt aatcacaaag gaacttacac caaaaacgaa
      361 agaatcaatc attgacagta aatgcaaaca gtatattttg attgaagaag caaacaagaa
      421 ggatacaaga atttattcat ccagcttcgc tggtgaacaa attgttcctg atgtcagcta
      481 ctggacattg ggagagtatt taaaacttat caaatcgtaa gacatggcaa tttttaattt
      541 tcaaaacgat gcggcaattg gaacagtgtt cagtgtagac actgcaactg taattatcaa
      601 ggttgatgaa attgataaac tgcgaaagtt gcatgtcaat catttggttg ctatcaagag
      661 ttcaaaagcg ggacaacatc tgataggtat tatcaatcgt atcacacgaa aagcatctga
      721 cgaagaaaaa ccaaccgaag aagatttggg tttaggtggg tttcttttca ctgaaaatat
      781 tttgaaagta aatcttatcg gaacacttca tgataagcac ggacagaaag aaaatgtttt
      841 caagagaaac cttgacacag ttcctgaaat tgaagcacaa tgttttccta ttgacggaga
      901 aaacataacc aagttcatga caacaatttc aagtgttgac aattataaag tccctctgac
      961 aattggaaaa tattctattg acgaacatgc acaggcattt ttagatggag acaaactttt
     1021 ccaaagacat gcggtaattg ttggtagcac tggttcaggt aaatcatggt gtgttgcaaa
     1081 gttccttgaa cagattgcac aactaccaat ggctgattgc attttgtttg acattcacgg
     1141 agaatacagc ggaaaagatt ttaaaatgga tggtgtgcaa cacttgcgtg ttgccaatcc
     1201 atctgactta ggtaagaaag ggaatcttgc aaataatgtt ttgatgttgc cctactggat
     1261 gctcacttat gaggaaatgc ttgcaatgct tcttgacaga agcgatagca acgcaccgaa
     1321 tcaagcaatg cttttttcaa aaaccgtttt tacggagaag caaaagtttt tggatgctat
     1381 aaaagataaa atatttaaag accacattac gattgatagc cccattcctt acaaaatgga
     1441 aaatgttttg gatgagttac agaggatgga tgaagaaatg gttgctggtg ctaaagtggg
     1501 aactgaaaag caaggaccgt ttcatggaaa acttacaaga tttattcaac ggcttgaagc
     1561 aaaaagtcaa gacaagcgtt taggatttct gtttcaactt tctgaagatg aattgcagat
     1621 tgattggatg aacacacttt gcgaacgctt gatgttcggt tctgctttaa gcaaagaaaa
     1681 atcaggagta aaaattattg acttctcaga agttccttct gatgtattgc ctcttgtaat
     1741 cggattagtt gcaagagttg tatttacagt tcagcagtgg acagcaaaag aaaatcagca
     1801 ccccatttca ttactgtgtg atgaagcaca cttgtatatt cccgaaagaa caaatcaaga
     1861 tgcagcagca gaattagggc taaagagttt tgaacgaatt gcaaaagaag gaaggaaata
     1921 cggagtgagt ttgactgtaa taagtcaaag acctgcggaa gtaaaccgaa ctgttttaag
     1981 tcagtgtaac aacttcattg cattgcgact ttcaaatgca gaagaccaag cggtaatcaa
     2041 aaaacttttg cccgataatc tttcgggact tactgatgtt ttgccgatac ttgacattgg
     2101 agaagcactt gtagtaggtg atgcttcatt gcttccaaca agagtaatta ttgacgaacc
     2161 aaaaattaaa cctgaaagtg cgacaattaa attttggcaa gagtggagca atgacaaagc
     2221 aaaacaagat attgcaacgg cagtaacagg attaaggaaa caatcaaaag cataattaaa
     2281 aaatataaac atggcagaag aatatttaga catctacaac tcaaaaggag ttcacttagg
     2341 aaaggaactc aggaaagtag ttcaccaaaa aggaatgtgg cacaaaacat ttcactgttg
     2401 ggtgattttc cgaaatgaag caggtgagga ttgtatcctt atgcaaaagc gagctgccaa
     2461 aaaggaaagt gctccaaaca aacttgattc agcagtagcg gggcattaca cagcaggaga
     2521 agatatttcg ggaggcattc gtgagtttaa agaagaaaca ggaattgaca ttgattctaa
     2581 agatttaatt cctcttggaa tcagagttac tgtttctgat tacaaaccaa acagcatcaa
     2641 caaagaattt caagaagttt acttcattaa gaagtcaatc cctttagaat cattcaaact
     2701 cccagttgat gaagttgcag ggatggttga attgccagtc aaaaagtgca tcgcactttt
     2761 agacaaacag attgaccact tttttgcaag gggaattttt aatgatgatg cttcatcacc
     2821 gaacatagca ataatcatag aaagaaaaat cacgattgac gattttattt ggttcattga
     2881 caattttcat ttcaaaataa tgctgctaac aagcaaagca ttagcgaacg aaaaatatct
     2941 ctttatctaa tgttacttga aaacctgagc atagaaaaac ttccctattt ggagcggtat
     3001 gtaaacagtg gttcgccaag tggttttaca aatcgttaca ctacttccga agaaacaaaa
     3061 ccctttgggt tatcgccttt ttttcatcct ttgatttgtg tcgcacctga agaatgtttt
     3121 aaaaactatg gcaagatacc agcgttcaaa gagtttgaag aagttacagg aaaaaattgg
     3181 ttgcttgttc atcccgacat gaaaaaagag aaggtattta aagcaaaaaa tgtttcactt
     3241 acaaaatcaa agaagataaa attagttccc accgcaagcg gaagaacagt tgagcttttt
     3301 aattcatcaa ccaagggtta tttcaaactt cattacacac atgttatcgg acgaataaca
     3361 agagaattgg ataataaaat tggagttgcg ggacctgaaa tttcaaagca tcttgtttcc
     3421 ttgatagacc aaaacaaact tgacagcaga ttaaccttga tgcgtgaaac tggtgcaaga
     3481 atttttcagg ggcatgtaaa cgagaaacct gttgagtggg gaatggtttg gcgagagaaa
     3541 gatgcttaca agattgacta caacgaagtg aaatatgtaa ttcctgtttt ttcacttttt
     3601 ggtaaagaca tactggcgaa aagagataag ccgctattgc agcaaatcat taatctcatc
     3661 ggagcaaacc caactgacta tgttttaaag caactgattt tcccaatgat tgagctttac
     3721 ttcaacttga ttaaaaactc aggcattcaa cctgagtggc atgctcaaaa tttgttgata
     3781 ggttttacaa aagattttgc tcctgtaaaa tttatcatga gagatttaga atcaatggac
     3841 aaggacttaa cattcatgga ggaacataac atcaaggcaa aatttgaaag ttatcccttc
     3901 aaatgtattt tcagagagca gtggaattat caaaggaagc acagttttat gtatgactat
     3961 aagtttgggg aatacatcat tagaccgctt ctggactttc taaagaaaaa atacaaggtt
     4021 gacacattgc ccttacatca aaagataaaa cttcattcaa agaaattcat taatgaattt
     4081 tcaaaagatt tttttccaga gtattgggtt gtttttagaa agattttgat taaccaaaag
     4141 acagataagc gtccgtatgt tcataggaag aatcctaaat tcagatatta aatgcacata
     4201 gtttttatca gacataccga atcagaaaag aatgtaaaca accagttctc atcttacaag
     4261 gataaagaac cgttgactga aataggcatg agtcaaagcg aaaatcttgg caacgattta
     4321 ttggagttta aagaaagaaa aaacttgact tgcaagaatg tttatgcagc aagttctaca
     4381 agagcaattg aaacagcaaa aattattgct tctaaattaa atgctactgt aagtgttgag
     4441 gatgctctcc gttctacaaa gccaggagca ttatcgggca agtcagaaga ggaaggatta
     4501 aaaacaaatc ccgatttctt agaacaactt tatctattca gaaacgggtt gttcaatgct
     4561 tacgacttca ctgtagcgga aggaaaagaa cccaaaaaag attttgagaa aagagtttgg
     4621 ggaagattga gtgaaataat atctgacaag tcagagagtt tgaaaattat ctctgcacat
     4681 cgttcatcca ttacatacat gctgttggag tttgcaaaaa gatattacaa ataccctcaa
     4741 aatatgtcag ggcatgtatt acttgatttg ggtaaaatat cacttgtgga aactgtagca
     4801 gaaaataaat ggaaaattac aagagtgaac tttgatagtt ccgaactaaa aacactttga
     4861 tatatgagcg aaaacaaaac aaacaaacat tcctatttat cagcactaat ccaacttgga
     4921 aaatttgcgt ggggaatatt cattggcatt tcaatattgg ttacagtggc gacaatggta
     4981 acggacttta aaatgacaaa ggaacatcct gttctcactg gcttggctgt tcttgctgta
     5041 acgattttta tttcactcat tttattggtg ataaaaatga ttaatgaagg cgttgaacaa
     5101 aaatcccctt tacctctttc taccgaattg gtgaatataa taaacacact ttacgaaaag
     5161 gaaaagtatt tagatgtagt gcggcttggt tctgctttaa gcagatattt atggctcaaa
     5221 ggaaaatatc atgaacgaat tgaaattggt gaacgagttg aagatgcagc aggaaaggaa
     5281 agtcgcattg aagaacaagt atcatctctg atagatgata ttggctggac ttacaacatt
     5341 atcggacaac ccgataaagc ggttgagaat atcacgagtg gaattgataa agctcttaca
     5401 aacgagttat tttattatgc tgctaaaggt gaacgacatt tatctggaat tgaaaaacac
     5461 aaaggacatc aagccgaatg cttgagccat ttacaaaagg caaaggaaca ttcagaaaag
     5521 attttggatt tgaccagtaa aaatgaaatg gaagccagtt tgtttttagc agaagcaaaa
     5581 tatctgtttg aattaggaaa attaaatgag gcagaagcac ttgcagaaaa agcaatagca
     5641 gtatttaaaa atgaccccga taggattgta aagattcatt cgctgttagg aaatatttat
     5701 ttgaaacaga acaaacttca aaaagcgaaa gatgaattca ataaaggata caatgcaaca
     5761 aaaagcacaa gagaagatga ggttgcaaaa aacttaaaag gtttagcaga catcgcattg
     5821 cttgaaagag acataaaaag tggattaaaa tatttgaacg aagcaaaaga aatctatctt
     5881 tctattcaca agaacaaaga ggcaactgac ttggataaca tcattcgcag tatcaggatt
     5941 taagcaatta agatttagga caaatgccaa gtttgatttt aacctcagat gattttggtt
     6001 taagcaaaat atttaatcag cgaatgattg aattgctgca atcaaatttt ctttcgtcaa
     6061 tttcaatcat gataaatcaa gtaacttccg accaacaaaa tcaaattgcc gagataaaaa
     6121 aaatgtatgc taaaaataat ttcagtttag gacttcattt agaggtttca gaagcagacg
     6181 atgaagcgac atatcaaagc caatggaata tgtttgaaca attactttca ataccacctg
     6241 actacattga tgttcacaaa gggcattttc aaaatgcaac cttcaactct attgcagagt
     6301 tttgtttgca aaaaagagtt cggttcagaa aatacaaaga gacgacagta aatgttagca
     6361 gccctgaaag ttctctcact gctacattca aagagttggg tgcaatcaaa gaatggattg
     6421 caaacttcaa aacaaactca acttatgaat tggtttttca catcggtagt tttgaccccg
     6481 acagtaaatc cagtttgaat aaagacagag aacttgatat tgaaaagtta aaacatgttc
     6541 acgaatttat ttcagacaaa aatatttcaa taacaaacta caaatcgctt taaagaaaat
     6601 gccaacgcag cattcacaca catttgcaag ccgctacaag ccaacgctga aaccaaaact
     6661 tgcaaaagag tgtgtctgcc ctacccaaga agaatttgaa aattttttct ccaccccctc
     6721 aaataaaatt aaatcaccca accgcacagt gctcactcgg acagacatcg ccttgaatgt
     6781 aaatcgcctt gtgggacagg tttgtgcaga caatggacag acactatttc attgcgagaa
     6841 gtcgggtgat acgcataaca agggcttaaa cgaaagcggg ggttccgtgc ttcgcagaca
     6901 catttgtgca aggtggaagt tcagttctcc gaatgaagtt tagtgctaca actcaccctc
     6961 tggcgcaagt cttcggactt gtgcccatct atcattcaag cagcagtaag tccaataaac
     7021 ctttggtgcc gcaataatca catgcactgc tgtaaaaata atcttccttg taggcgcgag
     7081 cgtcattttc cttactttca aagcctctcg catttcaggg gactttgaaa taataaaaat
     7141 agcattgaag ggttaataca ttttttatga atatagaata catgtagttt aaaattttgc
     7201 atgacgacta cacgtagaaa atatgatgaa cgtgaagtac ggcattatcg ctctcccaac
     7261 tggaacaaac taactttgtt ttaatatgat atagtgtgta cctgataggt tcaatccaca
     7321 tttttctgtc gcagtttttt tatttctgct gttctgcgtt tttgagtcag tcgttcttct
     7381 tttacctgtg ccggtatttt tgtacttatt cgttttgcag gtctatgcat agctttttca
     7441 atcagcattt gaaatttttt caataccagc tctttattac ctaactggct acgtgcctca
     7501 ctgctttgaa gttttaagaa accatcttcg ttgattcttc ggcaaagttt ttccgtaata
     7561 actttttttt gttcctcatc taaacataca gagttttcaa tatcaaaggc aagttcagcc
     7621 ttggtagaaa ccttgtttac attttgtcca ccttttcccg agctgcgcga gtaggtcaat
     7681 atcacttctg aaaaaagaca tttaaaatct atttttccca ttacagttta atggtaaaac
     7741 tatcggcatc attgcccaca ggaaaaatgt gccttgcatt ttccaaaaca tcaaagtcga
     7801 gttttacagt ttctatattt tctgttgcag attttgtttt gctgattttt ttaccaaacg
     7861 gatcgagaac tactgtatct ccggaataag taatgtcatt tccatctttt ccaatgcggt
     7921 taacaccaac aacaaacgat tgattttcta tggcacgtgc cgtcaacaat tgtttccagg
     7981 caaagctgcg tttctcaggc cagttagcta caacaatagc acaatcatat tcgttctgat
     8041 tacgcatcca cacaggaaaa cgcaggtcgt aacaaataaa aactgagagt cgccatcctt
     8101 tataaagcag aattaatttc ttgtttccag gatgataaaa tttttgttca ccagccatag
     8161 taaacaaatg ccttttatcg taatgtgcat gagcgccacc gggagtcatc catactaagc
     8221 ggttataata tctgccacct tcttcaacaa taagacttcc gcaaactaca gcattttttg
     8281 tttctgccat tttttgcatc cactgcatgg tagggccatc atttttttct gccagtacgc
     8341 ttgcattcat gctaaagccc gtactaaaca tttcgggcag cacaattagg tctgtgctac
     8401 ctgctttaat acttttaagc agtgtagtaa aatgtttaat gttttttgcc tgactttccc
     8461 agtgaagctt tgtctgaaca gttgttactc ttagagtgct catggtatga tgttgttggt
     8521 tcaggcaaag ttatatttta taattgcaga aaaggtatta tttttcaacc ttatgcagcc
     8581 tttcagcagc tttttcaagt gtttcttcag attttgcaaa acaaaaccgc aaaagtttgt
     8641 ggtctgtgcc gtttctgtaa aaagccgaaa ccggaattga tgccactcca tagtcttttg
     8701 tcagttttac ggcatagtcc atatcttttt cattactcag tttactgtaa tcgagcaatt
     8761 gaaagtacga acctgagcag gccaatggct taaatgcagt gccttgaatc agctttaaga
     8821 aattgtctct tttttcctga taaaattttt caatacctga atagttttta gcatccttca
     8881 taaattctgc ataggcatat tgaatgggag tgttggcact aaaaaccata aactgatgtg
     8941 cttttctgaa ttcggcagaa agttttgccg gggcaatgca gtatcccatt ttccaccctg
     9001 tggtatgaaa agtttttccg aaagaataaa cgataaaact gcgctcggca agttttggaa
     9061 aacgcgctac actctgatgt tcgtaaccat caaaaataac atgctcatac acttcatcac
     9121 tcaagacaaa aatgtctgtc tttgcagtaa ttttttcaag catctgaaaa tctttaacac
     9181 ttaaaatagc acctgtaggg ttatgtggtg tgttaatcat aatcattctg gtgcgttggt
     9241 tcatgagttt tttcacttca ttccagtcaa tatggaagtc gggcattttt aactctgcat
     9301 gaaccggttc gccaccggca agaacaacag caggtgcata ggaatcataa gcaggatcaa
     9361 aaataataac ctcatcgcct tcgcgtacta cagatgctat ggctgtataa atagcttgtg
     9421 ttcctcctgc agtgatagta atttctgttt caggattata ttttgcacta aagcgggctt
     9481 ctattttagc agcaatttct tcgcgcagcg gcatgatgcc cggcatgggt gcatattgat
     9541 tcatgccttt tttcatatac ttatttacca gactaatcag ttcgggcgaa atggcaaagt
     9601 cgggaaagcc ctgagaaaga ttaatggcat tacactcgtt ggcaagtgca gacatgacac
     9661 taaaaatgct gtttccggtt ttaggaagtt ttgattttat taatgcttga tttggtgaca
     9721 taaaaagcgg ctatgcttca aatgtaaatc aaatgccgtt aacaaaaaag aaatctgctt
     9781 tataagctcc tgctttaatg ctattgtatt gagtataaca gcaaatcgtt ttaaacaaca
     9841 ttccctttca cagattttta tttaagattt tgatggttgt agaattgaag tcttttgttt
     9901 atagttagca gtttttaaaa tcttcattga tttgaattca attatatttg tttgctcagt
     9961 taatgtttgt attttgattg aatgaaattt ccatcaatta atttttctgt ttatggtaaa
    10021 agaacatatt tggttcttgt aagtgtattg atcggtgctt taactacttt atgtgcagcc
    10081 ttattaaaaa aaggtgtaat gctgatggag cacattgcag agcatttggt ttttgattcg
    10141 ggctatcatc agttgttttt gattttgccg gcaataggaa tattgctaac agttacattc
    10201 tggagactga tgaaaattaa aaatgaacga aggggaatga cggcagtaat ttataacatt
    10261 tccaagcagt cgagtgttgt tgccaggcac aaactgtttt cgcagatggt tagcagtatt
    10321 tttactgttg gttttggtgg ctcagcaggt cttgaagcac caatcgcatc taccggttca
    10381 gctattggaa gcaatattgc aagcaaatta aaattctcta ctaaagaaag aacattgttg
    10441 ctggcatgtg gtgctgcagc aggaatttct gcaattttta atgcacctat tgcaggtgtg
    10501 gtgtttgcac tcgagctttt gctcattgat atgcctgtac ctgttgtcat accactattg
    10561 atatcaagtg ccactgatga ttttttatca cgatatttta atctcgtcca gccttttgtt
    10621 ctcataaccg acacatggaa ttcaagtcac atactctatt accttgcttt agctgcattg
    10681 gcagctataa tttctattta ctgtattaag atatattttt tcatcagctc attgttttca
    10741 aaaatcacca atgtatttgt aaaagcactc attggtggaa cattacttgg actgctgata
    10801 tttttatttc caccgcttta tggtgaaggg tatgacagtg ttcagttgtt attgcacaat
    10861 caatctgcag aaatttttaa aaacataacc ttttttgaag ctaatgcttt gtggctacca
    10921 attcttgcag cactgctgat gttttttcta aaagtagtag caacatcatt tacggttttt
    10981 ggtggaggta atggaggtat gttcgggtca tcattattta caggtgctat gctgggtttt
    11041 actttttcgc gagtggtgaa tattatgggt tgggcagagt tgaatgaaat aaattttaca
    11101 gtaattggta tggcatcaat gcttagtgga gtacttcaaa caccgctcac agcaattttt
    11161 ctgattgcag aaattacagg aggctatgct ctttttgttc ctttgatgat taccacatca
    11221 attacttatt tagctacacg atatgtagag ccacattctg tttataccaa acaattagca
    11281 cttaaaggcg gacttattaa tctgagtaaa gacaaacagg taatcagcaa aatgaaatta
    11341 agagattatt tagaaaccga tttagaaaca attaatatta atgcaactct tggtgattta
    11401 atcaaggcta ttcgtaagag caggcgcaat atttttcctg tggttgatgc tatgaatcat
    11461 ttacagggaa tcatattact tgatgatgta aagagcatca tgtttgatca gtcacagtat
    11521 gacaaaggag tcaaagaact gatgcaaagg ccaccattgg ttttagatat agatgaagat
    11581 atggaacgtg ttttagaaaa atttgaagag tatgaagcct ggaattttcc ggtaacggaa
    11641 aagggtgttt atgcaggttt tgtttctaag tctaagatat ttactttcta caggcagctt
    11701 cttattcagg agtctgacgt gcctatgcac gattagcgac agacttaaaa gtaaaaaagc
    11761 ccctttgcag gagctttttt ataattttca gaagattatt ttttcttctt agcagcagct
    11821 ttcttcttag tagccgcttt cttttttgga gccgcttttt tcttagtagc tgctttcttt
    11881 tttggagctg cttttttctt agttgcagct ttcttttttg gagcagcctt cttagcagaa
    11941 actacttttt tcttagtagc cgcttttttc ttagtggctt tcttcttagg agcagctttt
    12001 ttcttagcag ctttcttttt tggagcagct tttttagctt ctaccatttc agcacctgta
    12061 gtgctttcaa tcattgaatt gttcatcatg atgattattt atttattggt tattgatatt
    12121 gttatactca gagtttgctg ataagttaca tttttagttt acgtcaacaa ttttctctgc
    12181 cttatattca ccgtttaata atttcgattt tatttttccg aaagattcaa ttgtatattt
    12241 tacatcttct aatgtatgaa ctgctgtagg tattaaccta agcataataa ttccttttgg
    12301 tactacagga tatacaacca tcgagcagaa aatatcatag ttctcacgca agtcatatat
    12361 aagttttgta gcttcaggaa tagttccatt aagataaacc ggtgtcaccg gtgattgtgt
    12421 ttttccaata tcaaatcctg cttgcttgag tccgttctgc agggcattaa caattttcca
    12481 aagattgttt tt
//
LOCUS       DAORQT010000027        12478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_552_length_12478_cov_8.325847, whole genome shotgun sequence.
ACCESSION   DAORQT010000027 DAORQT010000000
VERSION     DAORQT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_552_length_12478_cov_8.325847"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1134
                     /locus_tag="PK134_02050"
     CDS             211..1134
                     /locus_tag="PK134_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37667.1"
                     /translation="MIDVINLKKSYGEREAVKSLNFLIKDGEFYGLLGPNGAGKTTTI
                     SIMSTILDADAGDVVIDNYNIKKNPKKVKQVIGVVPQEIALYNELSAGENLKFWGGLY
                     NVPESELKARMDELLKWFGLYDRRNDKVGSYSGGMKRRINIAAALLHRPKVLFMDEPT
                     VGIDPQSRNCIYEVIEKLHRDGMTIVYTTHYMEEAERFCDRIGIIDEGRIIAEGTLDD
                     LKKSKAIKEVISITFSKLSDTLVNELTSFWRDLKHQDDKILFYSNDIKTDLPKLVSKC
                     NQLSADIANIEIQKTNLETIFLSLTGKQLRD"
     gene            1136..2407
                     /locus_tag="PK134_02055"
     CDS             1136..2407
                     /locus_tag="PK134_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37668.1"
                     /translation="MIKLAIKDLMLFIKDKRSMVLTFAIPIALITLFSFAFGGAGKNK
                     EDQKITLLLSDLDKTKLSEAAIEKLDSLKSIQIVQIQLDSAQNAIKKGNESCVLIIHK
                     GFADSLLAGNVLPLELQYDEARDIEVGLLQQSLIPTVAMLPFDLGNTQQVMANKFQKM
                     VGNSSQNSKANIQMKSDSLFSAIYQGMANNNTGGGANASDAFFDSGIKMTKLIASKND
                     NALGLIHAVAGTSVMMLLFSVVGIGVGLLDEKQEGTLKRLLYSPIDPIHILFGKLITA
                     NIISIFQLVVMFAFSGLVFGLDVSAHLPGLIIAIVATAFACSAFGVFLASFAKSRQQV
                     QGLSTLIILVMSAIGGSMMPLFFMPAFMQKLAVVSVNYWSIQSFYDIFWRNLSITSVT
                     FLSRIGVLLLIGTILNIIAVYLFRKNILKLT"
     gene            complement(2440..3543)
                     /locus_tag="PK134_02060"
     CDS             complement(2440..3543)
                     /locus_tag="PK134_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRB37669.1"
                     /translation="MYLSNLSLVNFKNYTEAQLNFSSGVNCFTGSNGSGKTNIIDAVY
                     YLSFTKSYFNISDTQNIHHGESLFVIQGKFVVNEKEEDVFCGVKTGFKKQVKRGNEEY
                     ERLSDHIGLFPIVMVAPVDHILIAEGSDERRKFIDSIISQVNKPYLENLISYNKALMH
                     RNSYLKQLHGRMPDPSMMEVWDEQLVNYGSAIEKERRKFIAEFIPLFNETYNYLADHA
                     EEVSINYQTQLEQDDFRTLLKLSLQKDIALQHTSAGIHKDELVFKLGKYPLKRIASQG
                     QQKTFLMAVKIAQFEYLFRHKKTKPILLLDDIFDKLDDFRVKRLMELVSRHTFGQIFI
                     TDTHPERLKKIFEDISVNIKLFEVSQGMINEVS"
     gene            complement(3571..4695)
                     /locus_tag="PK134_02065"
     CDS             complement(3571..4695)
                     /locus_tag="PK134_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRB37670.1"
                     /translation="MKYIVTLCRILVGVLFIISGFIKANDTLGFSYKLDEYFLVFNMS
                     FMSAFSVALAQFICVAEIVLGVATLVGWRMNLVTWLLMLMIVFFTFLTFYSAYFNVVK
                     DCGCFGDALKLTPWQSFGKDVVLLVLILVIFAWRNKVKPLFELKKTRWVVIFALFASS
                     LFTYYTYAHLPVIDFRPYAIGNNLKIGMLPPPNARPDSVVIVFKYKAKDGTIKEFGMN
                     NLPEDLENYEFVDRTDKVIREGDKPKIHDFTIVDADGHDHTQEFLNNPQYSFMLVAYD
                     LNKSNTKIQSKVNQLADACSKNKVQFFGLTSTVAAETDLFRHENQNMFDYYFCDGTTL
                     KTIIRSNPGLVLLQNGVVVAMWHYNDFPSWDDVNKQYLKK"
     gene            4890..5669
                     /gene="folP"
                     /locus_tag="PK134_02070"
     CDS             4890..5669
                     /gene="folP"
                     /locus_tag="PK134_02070"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRB37671.1"
                     /translation="MGVLNLTPDSFYDGGKYNSENAILTQVALMLNEGATFIDIGGSS
                     SRPGSKMPTEEEEWSRIGNVIALIKQHFGDVLISVDTVYSVIAKRAVDAGACMINDIS
                     AGLMDSKMIETVAALKVPYLMMHMKGRPENMQDNPVYDNVLKEVIDFFSKQLLLCRKV
                     GINDILIDPGFGFGKTVEHNFELLAKLNLLKIVGLPIVAGLSRKGMIWKTLDITPSEA
                     LNGTTALNMVALMNGARILRVHDVKEAMQCIKLFNKLNEVR"
     gene            complement(5685..6344)
                     /locus_tag="PK134_02075"
     CDS             complement(5685..6344)
                     /locus_tag="PK134_02075"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRB37672.1"
                     /translation="MTIHREGKNWVWGTLITVLLINFLVLNFRAEHDWITVLVLIVTI
                     VFFLLILQFFRYPKRNITRNENYVIAPADGKVVVIERTEESEYYKDKRIQVSIFMSPI
                     NVHANWYPMSGKIKFLRYHKGKYLVAWHPKASTENERSTIVVEKDSNKTILLRQIAGA
                     LAKRIVYYPRENDLVKQGAEMGFIKFGSRVDIYLPLTAKINVELNQKTIGGVTVIAEL
                     A"
     gene            complement(6375..7652)
                     /locus_tag="PK134_02080"
     CDS             complement(6375..7652)
                     /locus_tag="PK134_02080"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRB37673.1"
                     /translation="MADSKKAKQNHEEENPFEEMIKRLDVAAKIMKLDSEVYEIMKKP
                     SKLVYCSMPVKMDNDKTKVFEGFRVVHSTALGPSKGGVRYSTYVNENEVMALAAWMTF
                     KCAVADIPYGGAKGGITCDPSTMSKGELERLTRAYTNAMVDVFGVDKDIPAPDMNTGP
                     QEMAWIVDEYSKLKGGFTPGVVTGKPIHLGGSLGRSEATGRGVMTATMEAMAKMGLNP
                     AKCRAAVQGFGNVGSITAKHYEAKGLKIVAISDHTAAFYNPDGIDIEKAIKYRNSNKG
                     VIKGFKGGKLISNEELLTLNVDVLAPCAMENQITDENAGKIKAKLIVEGANGPTTDEA
                     DHILSKKGTVVIPDILANGGGVTVSYFEWGQNRSGLYMTEEEVNTKADHWMKQAFHNV
                     WNTSVKHKTTMRIAAYIYALGKIELGIKSRGHY"
     gene            complement(7884..8903)
                     /gene="murB"
                     /locus_tag="PK134_02085"
     CDS             complement(7884..8903)
                     /gene="murB"
                     /locus_tag="PK134_02085"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRB37674.1"
                     /translation="MPMRENVSLKNLNSFGIEANARYYIDIHSEDDLILFLLQHKKTT
                     LPLLVMGGGSNMLFFKDFDGIVLKNNLHGIDVVEENEASVKIRIGAGEVWHNVVMWSV
                     EKNLGGIENLSLIPGTAGAAPIQNIGAYGVEIKNSLVAVEALAIDSAEKKVFNNHECE
                     FGYRNSVFKNAAKGKYIITAIILELKKQPQFKTEYGAIHSELEKQGIKNLSVKAISDA
                     VIAIRRSKLPDPAVIGNAGSFFKNPEITEQEFEKIKQQHPNIPHYPGAANKIKLAAGW
                     LIEQCGWKGFREGDAGCHKNQALVLVNYGNATGAEIYSLALKIKESVYNHFGVHIEPE
                     VNIIQ"
     gene            complement(8903..>10815)
                     /locus_tag="PK134_02090"
     CDS             complement(8903..>10815)
                     /locus_tag="PK134_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37675.1"
                     /translation="DSTGCMATDSIEIVNPAAATLQFSTTTATCFGSCNGSASVVSSG
                     TSPFTYLWNTTPVQTTDTATNLCAGTYFVTVTDSNLCAVTDSVVITEPVANLISFTAQ
                     LPACVSSCNGLLTATSTGTAPFIYQWSNGDSISTIDSLCTGLYTIIISDSIGCTVTDS
                     ISLNPAPPLPITFTTQFAGCNGVCTGAVSAAITGINPISYIWSTGDTIASIDSLCQGI
                     YTVSITDSLGCVVTDSAQIISQPVLLNPIATAPTCAGQCNGLASINPSGVAPFQWQWS
                     TGDTTSLSIDSLCAGVYYITITDSVGCVGSDSVTIVDPPAITYSASHTDATCANLCNG
                     ISTITATGNGTLTYLWNTVPPQTDSTNTNLCFGYTTYIITDTNNCSVTDSVLIFEPSP
                     IFIGNNFLGITCNGNCDGFVRALPSGGTPGYTYLWSNGVTFDSIFNTCAGTYTVTVTD
                     ANLCTAVDSFTFVEPDPIVINFTVIDASCPGCTDGSIIATATGGTPPYDYLYPALGIA
                     DSAATNLGMGYYLFCAQDFNNCMQCDSVFVDEATAINSLSPEIKEIKIFPNPFADKAY
                     LSITTAESKDFKLYFFDVAGRIVNIPYNHAGNTENKQVFIIQNNGLKAGLYYVKIMSA
                     NEIIAIGKFIVN"
     assembly_gap    10816..10854
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10855..>12478)
                     /locus_tag="PK134_02095"
     CDS             complement(<10855..>12478)
                     /locus_tag="PK134_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRB37676.1"
                     /translation="VQAQFWLPIGSGVNDNVYAMTKDTVNNILYIGGRFTDASGVTAI
                     RIASWNGTTYTAIGNGFDSNVFALYYDHSNNTLYAAGSFLFSGINPITRIAKWDGTQW
                     LPLGTGCNDEIYALTGDANGNIYAGGIFTTAGGVSTERIAKWNGSTWSALGSGIGSGS
                     NYVEALTFYNGDLIAGGQFGVAGGVSVSGIARWNGTNWFDLNGGVSGLSMRVSAFKEV
                     NGELICGGTFTAAGGVSSNSVAKWNGAAWLAIPGGITGGQAKVKSLGYLFNTLYVGGN
                     FSLAGGNAVSNITAYDGTTWADLSGGTNNQVDALLNYGNEMYVGGAFTMAGGNPATYI
                     AKYRTTCLTNASITTTQTSCFGQCNGTATVTATGNAPFTYQWSSTPVQTDSTAADLCA
                     GNYTVTITDNIGCSITQPVTITEPAQFAVTFSSNNPTCFGLCDGNALASNNGQGTVTY
                     NWNTIPAQTTQTATGLCEGTYSVTLIDSAGCMITDSVTIANPAPNTLTLTSSNPSCYN
                     NCDGIAAVASTGNAPFQYLWNTIPAQQTDTATG"
BASE COUNT         3851 a         2271 c         2284 g         4033 t
ORIGIN      
        1 gtatcaggtt cttctgacca tatttaaaat caatttttgt tgcccaaaca aagcttctgt
       61 aaagtttaaa ttgctgatag cttattttta atcagattat tttctttgct taatgtaaat
      121 ttcagttatt gaaatatact tttaaaaatt aattgctgat ttgatactca ataaaatcag
      181 ttttgttttt tctaatatac aatccctaat atgattgatg taattaattt aaagaaaagt
      241 tatggcgagc gagaagcagt aaagtcatta aactttttga ttaaagatgg tgagttttac
      301 ggtcttttag gccctaatgg tgcaggtaaa accacaacca tttctataat gagtacaatt
      361 ttagatgctg atgccggaga tgttgtaatt gataactata acatcaagaa aaatccaaaa
      421 aaagttaagc aagtcatagg tgttgtacct caggaaatag cgctctacaa tgaactttca
      481 gccggtgaaa atttaaaatt ctggggaggt ttgtataacg ttcctgaaag tgagctgaaa
      541 gcaagaatgg atgaactgct aaaatggttt ggtttatatg atcggagaaa tgataaagtg
      601 ggtagttatt caggtgggat gaaacgcaga ataaatattg cagcagcttt gttgcatcgt
      661 cctaaagtac tatttatgga tgagccaacg gttggtattg acccgcaaag cagaaattgt
      721 atttacgaag tgattgaaaa actccatcgt gatggaatga ctattgttta cacaacgcat
      781 tatatggaag aagcagaacg tttctgcgat agaattggaa ttattgatga gggaagaatt
      841 attgccgaag gcacgttaga tgacttaaag aagtcgaagg caattaagga ggttatctca
      901 attacttttt caaagttaag tgatacactt gttaacgaac ttacttcatt ttggagagac
      961 ctgaaacatc aggatgataa aattcttttt tattcaaatg atataaaaac agatttgccc
     1021 aagttggttt caaagtgtaa tcaattgagt gctgatattg ccaacattga aattcaaaaa
     1081 acaaatctcg aaaccatctt tttaagtcta accggtaaac aattaagaga ttaatatgat
     1141 aaagcttgct ataaaagatt taatgttatt tattaaagac aaaaggtcaa tggtgctgac
     1201 ttttgctata ccaattgctt tgataacact tttctcattt gcctttggtg gtgcaggaaa
     1261 gaacaaagaa gatcagaaaa taacactttt actcagtgat ttggataaaa caaaattgtc
     1321 ggaagctgca attgaaaagt tagactcgtt aaaatctatt caaatagtac aaatacaatt
     1381 agattccgcg cagaatgcaa ttaaaaaagg aaacgagagt tgcgtgctaa ttattcataa
     1441 gggttttgca gattctcttt tggccggcaa tgtattgcca ttagagttgc aatatgatga
     1501 ggccagagat attgaagtag ggttgcttca gcaatcgctt atacctactg ttgcaatgct
     1561 tccattcgat ttgggaaata cgcagcaagt tatggccaat aagtttcaga aaatggttgg
     1621 taattcaagt cagaattcaa aagcaaatat tcagatgaag tctgatagtt tgtttagtgc
     1681 aatttatcag ggaatggcaa acaataatac cggtggagga gctaatgcaa gtgatgcttt
     1741 ttttgatagc ggaataaaga tgacaaaact cattgcatca aaaaatgata atgcattagg
     1801 attgattcat gcagttgcag gaacttctgt gatgatgctt ttgttttcag tggttggaat
     1861 aggggtaggg cttttagacg aaaaacaaga aggaacttta aagagattac tttattcacc
     1921 cattgaccct attcatattt tatttggaaa attaattact gccaatataa tttcaatatt
     1981 tcaattagta gtgatgtttg cattttcagg cttggtattt ggtttggatg tcagtgctca
     2041 cttgccgggt ttaataatag caattgttgc cacggcattt gcctgttctg catttggtgt
     2101 ttttcttgca tcattcgcta aatcaagaca acaagtacaa ggactttcta ctttaattat
     2161 tttagtgatg agtgccattg gtggaagtat gatgccgctg ttttttatgc ctgcatttat
     2221 gcaaaaatta gccgttgttt cagtaaatta ctggagtatt cagagttttt atgatatttt
     2281 ctggcgtaac ctatcaatta caagtgttac atttttgtcg agaataggag tgttgttatt
     2341 aataggaact atactcaata taattgctgt ctatttgttc aggaaaaata ttcttaaact
     2401 gacttgatat aatgaagcca taaaatgttt acctgtaaat taacttactt cattaatcat
     2461 tccctggctc acttcaaaga gtttaatgtt tactgaaatg tcttcgaaaa ttttttttaa
     2521 cctttccgga tgcgtgtcag taataaaaat ctgaccaaat gtgtgacggc tgactagttc
     2581 catcagtctt tttactctaa agtcgtctaa cttatcgaaa atgtcatcaa gcaataaaat
     2641 gggttttgtt tttttgtggc gaaacaaata ttcaaactgc gcaattttaa ctgccatcaa
     2701 aaaagttttc tgctgtcctt gcgaagcaat tctttttagt ggatattttc caagtttaaa
     2761 aaccaattca tctttatgta tgccggcaga agtgtgttga agtgcaatat ctttttgtag
     2821 agataatttc aataatgtgc gaaaatcgtc ttgctcaagt tgtgtttgat aatttattga
     2881 aacttcttcg gcatgatcgg ccagataatt atacgtctca ttaaacaaag gaataaattc
     2941 agcaataaat tttcttcttt ctttttcaat cgcacttccg taatttacta actgttcatc
     3001 ccacacttcc atcatagatg ggtcgggcat gcgcccatgc aattgtttca gatagctgtt
     3061 tctgtgcatt agtgctttgt tgtagctaat caggttttcg agatatggtt tattgacttg
     3121 tgaaatgata ctgtcaataa actttcgcct ttcatcactg ccttctgcaa tcagaatatg
     3181 atcaacgggt gctaccatca caataggaaa aaggccgata tgatcactta gcctttcata
     3241 ttcttcattg cctcttttaa cttgtttttt aaatccggtt tttactccgc aaaaaacatc
     3301 ttcttctttt tcgttaacaa caaattttcc ctgaatgaca aacagcgatt caccatgatg
     3361 gatattttgt gtatcagaaa tattaaaata gcttttcgtg aaagagagat aataaacagc
     3421 gtcaataata ttggtctttc cactaccatt actgccggta aaacagttta caccggaaga
     3481 aaaatttaat tgcgcttcag tgtagttttt aaaatttaca agagataggt tagaaagata
     3541 cacttattgc agacaaattg attgatggaa ttattttttc agatattgtt tatttacatc
     3601 atcccatgat ggaaaatcat tgtaatgcca catggcaact actacaccat tttgcagcag
     3661 taccaatccc ggatttgaac ggatgatggt cttcaatgtt gttccatcac agaaataata
     3721 atcaaacatg ttttggtttt catgacggaa taaatctgtt tctgctgcaa cggtagaggt
     3781 caatccaaaa aactgaactt tattcttgct acaggcatca gcaagctgat ttactttact
     3841 ttgaattttt gtattagact tattcagatc ataagcaaca agcataaagg aatattgtgg
     3901 attatttaaa aattcctgag tatgatcatg gccgtctgca tcaacaatag taaaatcatg
     3961 tattttaggc ttatcacctt cacgaattac tttgtctgta cgatcaacaa actcatagtt
     4021 ttccaaatct tccggcaggt tgttcatgcc aaattctttt attgtaccat ctttcgcttt
     4081 gtatttgaaa acaataacca cactgtccgg acgtgcattg ggtggcggca acatacctat
     4141 tttaaggtta tttccaattg cataaggtct gaaatcaatt acaggtaagt gtgcataggt
     4201 gtagtatgta aaaagcgaac tggcaaataa tgcaaagatg acaacccaac gtgtcttttt
     4261 aagttcaaac aacggtttaa ctttattgcg ccatgcgaag attaccagaa taagtactaa
     4321 aagcactaca tcttttccaa acgattgcca tggagttagt tttaatgcat caccaaagca
     4381 accgcagtcc ttaacaacat tgaaataagc agaataaaaa gtcagaaagg tgaaaaatac
     4441 aatcatcagc atcaataacc aggtcactaa attcatgcgc cagcctacta aggtagcaac
     4501 accaagaaca atttctgcaa cacagataaa ctgtgccaat gccacagaaa atgcactcat
     4561 aaacgacatg ttgaatacta aaaagtattc atcaagtttg tagctgaagc caagagtgtc
     4621 gttggcctta ataaaacctg aaatgatgaa taaaacacca actaaaattc tacaaagtgt
     4681 aacaatatat ttcatacttt ctgtttgtta ttaatgtctt ttaaatcaat acaaataatg
     4741 ttgttttatg ccaattgttt tcaataattg acaaaaactt tttcacacct gatgtcaatt
     4801 gatatttttg ctctgtaaat cagtatatgc aaacatttac ttctataaac tgctgtggca
     4861 aattaatcaa tctgcataaa cctcatgtta tgggtgtatt aaatcttaca ccggattctt
     4921 tttatgatgg aggtaaatac aacagcgaaa atgcaatttt aacacaagta gcattaatgc
     4981 tgaatgaagg cgcaacattt attgacattg gtggttcttc ttcgcgtccg ggctccaaaa
     5041 tgccgactga agaggaggag tggagcagaa taggaaatgt aattgcatta attaaacaac
     5101 attttgggga tgtattgatt tctgtagata ctgtttattc agtaattgca aaacgtgcag
     5161 ttgatgcagg tgcatgcatg attaatgata tttctgccgg tttaatggac agtaaaatga
     5221 ttgaaacagt tgctgcactt aaagttccct atttgatgat gcacatgaaa ggccgacctg
     5281 aaaatatgca ggacaacccc gtttatgata atgtgcttaa agaggtcatt gatttttttt
     5341 caaagcagtt attgttgtgc aggaaagttg gcataaatga cattttaata gacccgggtt
     5401 ttggttttgg caaaactgtc gaacataatt ttgaactgtt agccaaactt aatttgctga
     5461 agattgtagg acttccaatt gtagccggtt tatcaagaaa ggggatgata tggaaaacac
     5521 ttgatataac tccttcagag gcattaaatg gtactactgc actaaacatg gttgcattaa
     5581 tgaatggagc cagaatttta agagtacatg atgttaaaga agccatgcag tgcataaagc
     5641 tgtttaataa attaaatgag gtaagataaa tattgtcgga acaactaagc caattctgca
     5701 attacagtaa caccacctat agttttctga ttgagttcta cattaatttt tgctgtcagt
     5761 ggcagataaa tatccacacg cgagccaaat ttaatgaaac ccatttcggc accttgtttt
     5821 accaaatcgt tttctcgagg ataataaaca attctttttg caagggctcc tgcaatctga
     5881 cgcaataaaa tagttttgtt ggaatctttt tcaacaacaa tagtggagcg ctcattttct
     5941 gttgacgctt tcggatgcca ggcaactaaa tatttccctt tatgatagcg caaaaatttt
     6001 attttaccac tcatcggata ccagttggca tgtacattta tgggtgacat aaaaatagaa
     6061 acctgaatac gtttatcctt atagtattca ctttcttcag tgcgttcaat tactacaact
     6121 ttaccgtcag caggggcaat gacataattt tcattacgtg tgatatttct tttcggataa
     6181 cggaaaaact gaagtatcag taaaaagaaa actatggtta ctattaaaac taaaactgtt
     6241 atccaatcgt gttctgcacg aaaattaagt acaagaaaat tgataaggag gacagtgatg
     6301 agtgtccccc atacccaatt ttttccttca cggtgaatag tcatttttag tggattagta
     6361 ggcggtatgt tttattaata atgaccgcga gatttaattc ctaattcgat tttgccaagt
     6421 gcataaatat atgcagcaat acgcatggta gtcttatgtt tcacagaagt attccaaaca
     6481 ttgtggaaag cttgtttcat ccaatgatca gcttttgtgt ttacttcttc ttctgtcata
     6541 tataatcctg aacggttttg accccattca aaatatgata cagttacacc accaccgttt
     6601 gcaagaatat caggaattac aactgtacct tttttgctca gaatgtgatc ggcctcatca
     6661 gtagttgggc cattggcacc ttcaacaatt aattttgctt taatctttcc agcgttctca
     6721 tcagtaatct gattttccat tgcacatggt gccagcacat ctacattaag cgtcagtaac
     6781 tcttcgttag agataagttt acctccctta aatcctttaa ttacaccttt attagagttt
     6841 ctgtatttaa tggctttttc aatatcaatt ccatcaggat tgtagaaagc tgctgtatga
     6901 tcagaaattg ctacaatctt caatcctttt gcttcgtaat gttttgccgt aattgagccc
     6961 acatttccaa agccttgaac ggcagcacgg catttagcag gatttaatcc catttttgcc
     7021 atagcttcca ttgtggcagt cataactccg cgacctgttg cttccgaacg tcctaatgag
     7081 ccaccaagat gtataggttt tcctgtaact acacccggag taaaacctcc ttttaactta
     7141 ctgtattcat caacaatcca tgccatttcc tgagggcctg tattcatatc aggtgcagga
     7201 atatccttgt ccacaccaaa cacatcaacc atagcattgg tatatgctct tgtcaaacgc
     7261 tctaattcac ctttcgacat cgtagatggg tcgcaagtaa ttccaccttt tgcaccacca
     7321 taaggaatat cagcaacagc acatttaaag gtcatccatg cagcaagtgc catcacttcg
     7381 ttttcattga cataagtgct gtatctcaca ccacctttcg agggtcctaa agctgtgctg
     7441 tgaacaactc gaaaaccttc aaatactttt gttttatcat tatccatttt aaccggcatg
     7501 ctgcaataca caagcttgct gggttttttc attatttcat aaacttcaga gtctaatttc
     7561 atgatttttg ctgctacatc taagcgctta atcatttctt cgaatggatt ttcctcttcg
     7621 tgattttgtt ttgctttttt actatcagcc atgtcttata taatattgat tgggaacgca
     7681 aaagtaggaa agatttatgt gtttgcaaac catgcaggaa ggctgtactt ttttataggt
     7741 tttgcatgag atatatcagt ttatcgtatg taaatgcaag tttaaatatt actttactta
     7801 ctttttagaa tagtagccaa tgtcaatttt gacattcagt tgacaatgca ctttttatca
     7861 atgaaaaatc tatctgaaaa acactattga atgatgttta cttcaggctc aatatgaact
     7921 ccgaaatggt tgtaaacaga ttctttaatt tttaatgcga ggctatagat ttcagcacct
     7981 gtagcattac catagttcac cagtactaaa gcttgatttt tatgacatcc tgcatcaccc
     8041 tctctaaatc ccttccaccc acattgctca ataagccatc ctgctgccag ttttattttg
     8101 tttgcagctc ccggataatg cggaatattt ggatgttgtt gcttgatttt ttcaaattcc
     8161 tgctcagtaa tttcaggatt cttaaaaaaa ctacctgcat tgccaataac agcaggatcg
     8221 ggtaatttac ttcgtcttat tgctattacc gcatcactga tggctttaac actaagattt
     8281 ttaattcctt gtttttcaag ttccgagtga atggcaccat attcagtttt aaattgaggc
     8341 tgctttttca attccagaat aatagctgtg ataatgtatt ttccttttgc tgcatttttg
     8401 aaaacactat ttctgtagcc aaattcacat tcatggttat taaacacttt tttttctgca
     8461 ctatctattg ctaatgcttc aacagctacg agtgagtttt ttatttctac accataagcg
     8521 ccaatattct gaattggtgc agcacctgca gtacccggaa tcagcgaaag gttttctata
     8581 cctccaaggt ttttttctac cgaccacatg acaacattgt gccatacttc acctgcacca
     8641 attcttattt taacggaagc ttcgttctct tcaactacgt caatgccgtg caggttgttt
     8701 tttaaaacta taccatcaaa atctttaaaa aaaagcatgt tgctgccacc acccattacc
     8761 aataatggaa gtgttgtttt tttatgctga agaagaaaaa gtattaagtc gtcctcactg
     8821 tgtatatcaa tataatagcg tgcgtttgct tcaatgccga aggagtttaa attctttaat
     8881 gaaacatttt cacgcatagg catcagttga cgatgaattt accaatagca ataatttcat
     8941 ttgccgacat tatttttaca taatacagtc cagcttttaa tccattgttt tgtataatga
     9001 agacttgttt attttcagta ttacctgcat ggttataggg aatatttact attcttcctg
     9061 ctacatcaaa aaagtataat ttaaaatctt ttgattctgc agttgtaata cttaagtaag
     9121 ctttgtcagc aaatggattc gggaaaattt taatttcttt tatttccggt gaaaggctgt
     9181 ttattgcagt agcttcgtca acaaaaacac tatcacattg catgcaattg ttgaagtcct
     9241 gtgcacaaaa taaatagtaa cccataccaa gattggttgc tgctgaatct gcaattccta
     9301 atgctggata caaataatca tacggtggtg tgccacctgt tgcagttgct ataatgctgc
     9361 catctgtaca acctgggcaa gaggcatcaa ttactgtaaa attgataact attggatcag
     9421 gttcaacaaa agtaaaagag tcaacagctg tacatagatt ggcatctgta acggtaactg
     9481 tgtaagtgcc tgcacaggta ttaaaaatag aatcgaaagt tacaccattc gaccataaat
     9541 aagtatatcc gggagttcca cctgatggaa gagcacgaac aaaaccatca caatttccat
     9601 tacaggttat tcctaaaaaa ttattcccga taaatattgg tgaaggttca aaaatcaaaa
     9661 cagaatcggt gacagaacag ttgttagtgt ctgtaattat ataggtagtg tagccaaagc
     9721 acaaattagt gtttgtagaa tctgtttgtg gaggcacagt attccataaa taagtcagag
     9781 tgccattgcc ggttgcagtg atggttgaaa taccattgca tagattggca caggttgcat
     9841 ctgtatgcga tgcagaataa gttattgcag gtgggtcaac aatggtaaca gaatcagagc
     9901 ctacacaacc aacagaatct gttatggtga tgtaatatac acctgcacat aaactgtcaa
     9961 tagaaagtga tgttgtatcg cccgttgacc attgccattg aaatggtgct acgcctgatg
    10021 gatttatcga agccaatcca ttgcattgtc ctgcacaggt tggtgctgtt gcaattggat
    10081 ttagtaaaac aggctgactg ataatttgtg cagagtctgt tacaacacat ccaagactat
    10141 cggtaatgct tacagtataa ataccctgac aaagtgaatc aattgaagca atggtatcac
    10201 ccgttgacca aatatagctt attggattga tgcctgtaat tgctgcgctt actgcgccag
    10261 tgcacacacc gttgcatcct gcaaattgtg ttgtgaatgt aatcggaaga ggaggagcag
    10321 ggtttagtga aatagaatct gttactgtgc agccaatact gtcacttatg ataatggtat
    10381 aaagacctgt gcagagtgaa tcaatagtgc ttattgaatc accgtttgac cattgataaa
    10441 taaatggggc tgttccggtt gatgttgcag ttaacaagcc gttacaagaa ctgacacatg
    10501 ctggaagttg agctgtaaaa gaaatcagat tggccacagg ctctgtaatc actacggagt
    10561 cggttacagc acacaagtta ctgtcggtaa cagtaacaaa ataggttcct gcgcataaat
    10621 ttgttgccgt atctgtggtt tgaacaggag ttgtgttcca caaataagta aatggtgaag
    10681 ttccgctcga aacaactgat gcagaaccgt tgcaacttcc aaaacaagtt gctgttgtag
    10741 tgctgaactg taatgttgct gctgcaggat tgacaatctc aattgaatct gtagccatac
    10801 aacctgtact gtcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccctg
    10861 ttgctgtatc ggtttgttgt gccgggattg tattccacaa atattgaaaa ggagcatttc
    10921 ctgttgatgc tactgcggca atgccatcac agttattata acatgaaggg tttgatgatg
    10981 ttaatgtaag tgtgttagga gcagggttgg ctatagtaac tgaatctgtt atcatacaac
    11041 ctgctgagtc aataagcgta actgaataag tgccttcaca aagacctgta gcagtttgtg
    11101 tagtttgtgc aggaatggta ttccagttgt atgttacagt accttgtccg ttgtttgatg
    11161 ctaatgcatt gccatcgcac aatccaaagc aggtagggtt gtttgaagaa aaggtaactg
    11221 caaactgtgc aggctctgta atcgttacag gttgtgtaat gctgcaacct atattatcag
    11281 taattgtaac tgtataattt cctgcacaaa gatcagctgc tgttgaatca gtctgaacag
    11341 gagtagatga ccattgatag gtaaagggtg catttcctgt tgctgtaact gttgctgttc
    11401 cattgcactg accaaaacaa gaagtttgtg ttgtagtgat ggatgcattg gttaaacatg
    11461 ttgttctgta tttggcaatg taggttgccg gatttcctcc agccatagtg aaagcacctc
    11521 caacatacat ttcattgcca taatttaata atgcatcaac ctgattgttt gttccaccgc
    11581 taaggtcagc ccatgtagta ccatcataag ctgtaatatt tgaaacagca tttccgcctg
    11641 caagcgaaaa attaccacct acataaagtg tattaaataa atatccaagt gattttactt
    11701 tagcttgtcc tccggttata cctccgggaa ttgctaacca agcagcacca ttccatttag
    11761 caacgctatt ggacgaaacg ccacctgcag cagtaaaagt tccaccacat atcaactcac
    11821 cgtttacttc tttgaaagca gacactcgca tagacaatcc actaacgcca ccattcaggt
    11881 caaaccagtt ggtgccattc catcgtgcaa tgccgcttac tgatacacca cctgctacac
    11941 caaactgtcc acctgcaatt aaatcaccat tgtaaaaagt caatgcttca acatagttag
    12001 agccactacc aatgccagaa cctaatgcac tccatgtact gccattccat ttagcaatac
    12061 gctcagtgct tacgccaccg gctgttgtaa atattccacc ggcataaatg tttccattag
    12121 catctccggt aagtgcataa atctcatcat tgcagcctgt gcctaatggt agccattgtg
    12181 tgccatccca ttttgcaata cgtgttatcg gatttattcc tgaaaaaaga aacgagcctg
    12241 ccgcataaag tgtattgttg ctatgatcat aatatagcgc aaatacatta ctgtcaaatc
    12301 catttcctat agctgtatag gttgttccat tccatgatgc tatgcgtatg gcagtaacgc
    12361 cactggcatc tgtaaatctt ccaccgatgt aaagaatgtt gttaacggta tccttggtca
    12421 ttgcataaac attatcgttt acgcctgagc caatgggtaa ccaaaattgt gcttgtac
//
LOCUS       DAORQT010000028        11981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_616_length_11981_cov_8.996059, whole genome shotgun sequence.
ACCESSION   DAORQT010000028 DAORQT010000000
VERSION     DAORQT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_616_length_11981_cov_8.996059"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PK134_02100"
     CDS             complement(<1..1272)
                     /locus_tag="PK134_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRB37677.1"
                     /translation="MFAVVDIETTGGSLTQGSRITEVAVVIHNGNTIEHRFSTLINPE
                     TDIPSFIIGLTGITNELVENAPCFKEIAEQLFQLLDGKIFVAHNASFDFSFIKSEFKR
                     CGIDFNAEVICTCSLSRKIFPGFGSYSLGNLTKNLGIILEGHHRAMNDAVAASEILEK
                     LVSEKGLEHTLLHRSVPIKNTEIPHELISKLPETPGIYFFTDSNHEILYIGKAKNIKK
                     RVTSHFRNNNSKSKKIKALLHDISYMNTGCELLACLLEIEYIKEYQPFFNRAGKSEKN
                     FGVYKNDILNGLSKFFIHDISKTNSSPLRYFGSRKAADEFLSHITEKYSLCRCINSLE
                     SYSDNCFYKQIKQCNGIELDNNEKNNYNNRFNDACLEVGDGWHDAALAYPIAGSEEWA
                     YVFRHQGAIGYYFGKKATTFNINLIKGVTHLK"
     gene            complement(1283..2239)
                     /locus_tag="PK134_02105"
     CDS             complement(1283..2239)
                     /locus_tag="PK134_02105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRB37678.1"
                     /translation="MHKILIVQTAFIGDVILATGLIESLHQAFPESKIDFVLRKGNES
                     LLKSHPFVNKLYVWDKKKNKFSNLINLISQIRKEKYDLVVNLQRFFSTGLITLFAKSK
                     NKRGYNKNPLSFCYHKKSEHIIGKKHETYRNFSLIDDLKNIQYQKPNLYFTEEENQKV
                     QSLKSKPFVCMAPASVWFTKQLPVAKWAELTLTIPENIKIYFLGAPADVALCNEIRLL
                     SSHPNIEILCGKLTLTESAALMQHAEMNYVNDSAPLHLASAVNAPVTAFFCSTVPEFG
                     FGPLSDKSIIKQVGEMSCRPCGLHGYKSCPLGHFDCAYKIKI"
     gene            complement(2251..3066)
                     /locus_tag="PK134_02110"
     CDS             complement(2251..3066)
                     /locus_tag="PK134_02110"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRB37679.1"
                     /translation="MPVREKTVNAIWENRALLTDESNKKVIRSVIEDLDKGNIRVAEQ
                     MNNSWRVNEWIKKAVILYFPIQKMEVHHAGPMEFYDKIDLKTGYENLGVRVVPHAIAR
                     YGSFVAKGVIMMPSFVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQASPVIIEDNCFIGSRCIVVEGVRIEQEAVLGANVVLTMSTKIIDVSGNTPVEYRGV
                     VPARSVVIPGSIPKTFAAGTYQVPCALIIGKRKQSTDNKTSLNDALREYDVAV"
     gene            3152..3580
                     /gene="ruvX"
                     /locus_tag="PK134_02115"
     CDS             3152..3580
                     /gene="ruvX"
                     /locus_tag="PK134_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRB37680.1"
                     /translation="MPRILAVDYGNKRVGLAVTDPMQIIATALTTVHSSKAVEFICNY
                     FNSEEVEEIVVGKPVQMDNTPSEIEKNILLFIKKLIDKCPSAKVIRYDERFTSVMAQR
                     TLLEAGLKKQQRQNKALIDKTSAVILLQSYLEHKRNFLKI"
     gene            3577..4137
                     /gene="def"
                     /locus_tag="PK134_02120"
     CDS             3577..4137
                     /gene="def"
                     /locus_tag="PK134_02120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRB37681.1"
                     /translation="MILPIIAYGDPVLKKVGSSIDKDYKGLTQLIENMFETMYAASGV
                     GLAAPQVGISIRLFVVDTKPFAEDEPELENFKKVFINPQIIEEVGDEWKFSEGCLSIP
                     GIREDVMRKKIIHLRYQDEEFKTHETVFEGLAARVLQHEYDHVDGKLFVEKLSPFKKQ
                     LIKGKLRDITIGKVDVDYKMRFPIKK"
     gene            4143..4655
                     /locus_tag="PK134_02125"
     CDS             4143..4655
                     /locus_tag="PK134_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRB37682.1"
                     /translation="MLIFLIGFMGAGKSSQAKKLAARLNFESIDLDELIEKTVKQSIS
                     EIFKISGEQHFRKIESETLKTLSEKQNAVIATGGGTPCFFDNMQWMNEKGITVYLKIH
                     PGTVYHRIAPSKSKRPLISHMNDADLMEYIMETMIERAPFYNQAKIQMSGLNLKTEDL
                     VSALKPFLKD"
     gene            4715..5224
                     /locus_tag="PK134_02130"
     CDS             4715..5224
                     /locus_tag="PK134_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37683.1"
                     /translation="MKTLLCFLLLPTILVAQTFQKNQSEGNEDFIKRIAPHSANEVKY
                     LLSDSFNTSTEKIVYAYSIFEHGNLITNNDSTLTYFLCILVPTSVGALDYNMQQFRMV
                     ASYKKKARIESAEIIKSGREKKLQVYIAEMVRGPGGVPRDVKRTFVYKQEKESRMFTD
                     TFREVLGDY"
     gene            5400..9371
                     /locus_tag="PK134_02135"
     CDS             5400..9371
                     /locus_tag="PK134_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRB37684.1"
                     /translation="MASQFIPVDFNPFEEVIPDNFFTTSEEQREIWTSVQIGGDSASL
                     AYNESVTLFLKGLCNHKHLSEACKNVVQRHQSLRSTFNTSGETFQVHEHVNLEIPYID
                     LTDLSPSDKDARILHFSQREVSIPFDLLNEPVIRFAIFKISNSETALVITAHHIVCDG
                     WSIGIIMQDISKFYSALANNTQPDLEPVVPFSTYVKRQEERKLNGEYKRTEDYWLNLY
                     STDVPTVDLPVNKARPAARTFNAKRFDITIDAGLVSDLRKLGAKNGSSYVNTLFAAFE
                     VFLFRLTSNPSLVLGLPAAGQSASGMYAMVGHCVNLLPIKSLVDENITFGEYLKARKP
                     LMFDAFDNQEFTMGSLLGKLPLQRDPSRIPLVPAVFNVDLGMTQGVSFEGLNYEYTSN
                     ARKFENFELFVNATGMGDKLIIECTYNTDLFEDEMMQLRMQEFVVLLNGLVEYVDSAI
                     KNLPLLTDDEKRKLFIEWPVVKENKLSNECIHTLIEKQASANPDTNAIWFNGQSITYG
                     QLNQRANQLAHYLIQSGVKQETLVGICHDRTDDLIVAMLAVLKAGGAYVPLDPNYPAD
                     RIAYILSDAKAPLLISSSKYIDQLGETGTKLVCLDKEVTEISKEKITNPDVEITPDQL
                     AYIIYTSGSTGKPKGVAIEHRNAVALIDWAQTVYSSEELAGVLASTSICFDLSIFEIF
                     FTLSNGGRIVLVKDAMALNALPENAGVTLVNTVPSAIAELLKLKDGVPSSVKTINLAG
                     EPLPVELVQKIYSQTTVKKVYDLYGPSEDTTYSTFTLRKPDGPYTIGKAITNSQVYIL
                     DKNRRPVPVGIAGELYMAGDGLARGYLYKPEMTEERFVANPFVLGQRMYKTGDLGKFM
                     SDGNIEFLGRIDNQVKIRGFRIELGEIESMLRLRKEIKELVVMAREDQPGNKILVAYL
                     STNAGKQIDVNELRQYLRTELPEYMIPSVFMILEYIPLTPNGKIDRRALPVPEVEVKA
                     ESANFEEPHNPLESLLVGIWSEVLGIDKIGIHDNFFELGGHSLLGIRMMGAIEQKTGV
                     KLDYPTLFRASTISQLTKVMNAEELNIDWPIVVPLQPNGKERPLFFVHMHNGNIQRWR
                     VLLKHLDKDQPIYAIQPRGLDEKYDYHYSVEAMASFYIDEIKKIQPVGPYRIAGLCFG
                     GTTAFEMARQLKTKGETTELVFMINNYAPPSNPMQYKIRETWDEFKGMTLEDKLEFAL
                     EKTKNAGKKVLSKLTKVFDSGSTTASEVKVNTKPDIRWVNSVALLNYQPEGEYSEKVI
                     LIKTGEEVAKHYDETLGWKRLISGEIEMHQITGSDNDTIITHKEYYTQLAQYLNQAIN
                     QIK"
     gene            9380..10090
                     /locus_tag="PK134_02140"
     CDS             9380..10090
                     /locus_tag="PK134_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRB37685.1"
                     /translation="MDLQLTPEQVFCNELLIQDNDCLVFIFCSDSFIAVESELKKLLS
                     NDETERLLKFKFEKDRQTYCIAHGLKRVLLAALLNTKEQVEICSHSNGKPFINHQKHN
                     MPLHFSLSHSEKMIALAFQKNSPLGVDIEKIQPADKYGNVIQHYFTSDEQKLISRSDN
                     DTFFKIWTQKEAIGKATGQGIANLPEIVIPDDYELSTASLGGYILSTCFFRGNEISFK
                     TIHAQDVLNQINILSLRP"
     gene            10157..11824
                     /locus_tag="PK134_02145"
     CDS             10157..11824
                     /locus_tag="PK134_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB37686.1"
                     /translation="MNKTSFHELSLSHEMLQAIDEMGFTEASPIQAQAIPVLMQGQDV
                     IGQAQTGTGKTAAFGIPLLEMLDPKVKATSALVMCPTRELAVQVAQEIKKLAKYKKGI
                     AVLAVYGGEQIQKQINALKRPVHVIVGTPGRIMDHLDRRTINLSKIQMVVLDEADEML
                     NMGFREDMEAILQHAPDERQTVLFSATMPKPILEITKRFQTNPKLVKVTKQELTVSSI
                     EQIYYDIPSSQKADVITQLVELHNLKLMLVFCNTKRKTDEVTEQFQQLGYKAEAIHGD
                     LRQNQRNNVLARFKKGDVNMLVATDVAARGIDVENVDAVFNFDLPLDEENYVHRIGRT
                     GRAGRSGKAFSFVSGRNDMAKIREIEDYSKVRIERHQLPSGKERIELAKIKLAARLKV
                     LIEEGDMEQYDNMFATFKQEGLTLHQLAAALLKMHFAPMLQQSQNKSSSKSSGREERS
                     GREERSGRGNRDSGGGKGKRGKHSGEADMIRLFLSVGKKDKVSKGDIVGAVSGLSNMS
                     SSNIGVIDIYDKFSFVEVNGEFLNDVLSNVNGNKIKGRKVNVEIAKD"
BASE COUNT         3888 a         2047 c         2328 g         3718 t
ORIGIN      
        1 ttttaaatga gttactcctt taattagatt aatattgaat gttgttgctt tctttccaaa
       61 ataatatcct atagcaccct gatgtcggaa tacgtaagcc cattcttcgc taccagctat
      121 cggataggca agtgcagcat catgccagcc atcgcctacc tctaaacagg catcattaaa
      181 acggttattg tagttgtttt tttcgttgtt atcgagttct attccattgc attgtttgat
      241 ttgtttataa aaacagttgt cactgtagct ctcaagacta ttgatgcaac gacagagact
      301 atatttttct gttatgtgcg acagaaattc atcggcagct tttcttgaac cgaaatatct
      361 taatggtgat gagtttgttt ttgaaatatc atgaataaag aattttgata agccatttaa
      421 aatatcattt ttataaaccc cgaaattctt ttctgatttg ccggcacgat taaagaaagg
      481 ctgatattct ttaatgtatt caatttcgag taaacatgcc aataattcac atcctgtatt
      541 catgtagctt atatcatgca agagtgcttt aatttttttc gatttggaat tgttgtttcg
      601 aaaatgtgaa gtaactcttt ttttgatatt ttttgctttg ccgatataca gaatttcatg
      661 attactgtct gtaaagaaat atatgccggg tgtttcaggc aacttgctaa taagttcatg
      721 tggtatttct gtatttttaa ttggtacact cctatgaagc agtgtgtgtt caagaccttt
      781 ttccgaaacc aatttctcta aaatttcact ggcagcaaca gcatcgttca tggcacgatg
      841 atgcccttct aaaattattc caagattttt tgtgagattt cctaaagagt aggagccaaa
      901 accggggaag atttttctgc tcaaagaaca agtgcagatt acttctgcat taaaatcaat
      961 tccgcaacgt ttaaattctg attttataaa actgaaatcg aaagacgcat tatgtgctac
     1021 aaaaattttt ccatcaagca actgaaacag ttgctctgca atttctttaa agcatggtgc
     1081 attttcaact aattcattag tgatgcctgt cagccctata ataaatgatg gaatgtctgt
     1141 ttcaggattt atcagtgtag aaaaacgatg ttcaatagta ttgccattat gaatcactac
     1201 tgcaacttcg gtaattcggc taccttgtgt taaacttcct cctgtagttt ctatgtcaac
     1261 aactgcgaac atgccacgtt acttaaattt taatcttata tgcgcaatca aaatgaccta
     1321 aggggcaaga tttatagcca tgcaatccac agggtcggca gctcatttca ccgacttgtt
     1381 taatgatgct tttgtctgac aaaggaccaa agccaaactc aggaactgta gagcagaaaa
     1441 atgcagtcac aggagcatta acagccgatg caagatggag tggagcagag tcgtttacat
     1501 agttcatttc agcatgttgc ataagtgctg ccgactctgt aagtgttagt ttgccgcata
     1561 atatttcaat attcggatgt gaagaaagca accttatttc gttgcaaagt gcaacatcag
     1621 ccggtgcgcc aagaaaatat atttttatat tttcaggaat agtcagagtt aactctgccc
     1681 acttggctac aggtaattgt ttggtaaacc aaacagaagc cggtgccatg cagacaaatg
     1741 gttttgattt taatgactgc actttttggt tttcctcctc tgtgaaatac aaatttggtt
     1801 tttgatattg aatatttttc aagtcatcaa tcaatgaaaa gttacgataa gtctcatgct
     1861 ttttaccaat gatatgctct gattttttgt ggtaacaaaa ggacaatgga tttttattgt
     1921 agcctctttt gttttttgat ttagcgaata aagttatcag acctgtgctg aaaaatcgtt
     1981 gcaggttaac aaccaaatca tatttttctt tcctgatttg actgatgagg ttaatgagat
     2041 tagaaaattt gtttttcttt ttatcccaga catataattt atttacaaaa gggtggcttt
     2101 tcaataacga ctcattacct tttcttaaaa caaaatcaat ttttgattct ggaaaagcct
     2161 gatgcagaga ttcaattaat cctgtagcta aaattacatc gccaataaat gctgtctgta
     2221 caattaatat tttatgcata cttgttagtc tcagactgca acatcatatt cgcgcaatgc
     2281 atcgttgagt gatgttttat tgtcggtaga ttgctttctt ttacctataa taagtgcaca
     2341 aggcacctga tatgttcctg ctgcaaaagt tttaggaatt gaaccgggaa tgaccacaga
     2401 acgtgcaggc acaacaccac gatattcaac aggtgtattt cctgacacat caattatttt
     2461 tgttgacata gtcaatacaa cattagcccc caaaaccgct tcctgttcaa tacgaacgcc
     2521 ttcaacaact atacatctcg accctataaa acaattgtct tcaataatta ccggtgatgc
     2581 ctgaactggc tctaaaactc ctccaatgcc aactcctcca ctaaggtgta cgttctttcc
     2641 tatctgtgca caactgccaa cagtagccca ggtgtcaacc attgttccgc tgtcaacata
     2701 agcaccaata ttcacaaacg atggcatcat gatgacacct tttgcaacaa agcttccata
     2761 gcgagcaatg gcatgtggca caacacgaac acctaagttt tcataacctg ttttaagatc
     2821 tattttatca taaaactcca tcggtcctgc atgatgtact tccatttttt gaatcggaaa
     2881 atataagatg acagcttttt ttatccactc atttacgcgc caactattat tcatttgctc
     2941 tgcaacccga atgttccctt tatctaggtc ttcgataact gagcggataa ctttcttgtt
     3001 tgattcatcg gtaagtaacg ctctgttttc ccaaatagca ttgactgttt tttctcttac
     3061 aggcataaat attatttttc agctacaaat ataaccacct taatgcattc attgcccgtt
     3121 gttttttatt tttgcatctt taaattcaga tatgccgcgc atacttgcag ttgattacgg
     3181 aaataaaaga gtaggtcttg cagttactga tccaatgcag attattgcca ctgctttaac
     3241 tactgttcat tcctctaaag cggttgaatt tatttgtaat tattttaatt ctgaagaagt
     3301 agaagaaatt gttgttggta aacctgtaca aatggataat acaccatctg aaattgagaa
     3361 aaatattttg ttgttcatca aaaagcttat tgataaatgt ccgagtgcga aagtgataag
     3421 atatgatgaa cgttttacct ctgtaatggc acaacgaaca ttgttggaag ccggactgaa
     3481 aaagcaacaa agacaaaata aagcattgat tgataagaca agcgctgtta tattattaca
     3541 atcgtactta gaacataaaa gaaacttttt gaaaatatga tattaccaat tattgcctat
     3601 ggcgacccgg ttttgaaaaa ggtgggctca tcaattgata aagattataa aggtcttaca
     3661 caacttatag aaaacatgtt tgagactatg tatgcagcat ccggtgttgg gttggctgct
     3721 ccgcaggttg gtatttccat cagactattt gttgttgaca ccaaaccatt tgcagaagat
     3781 gaaccggagc tggaaaattt caagaaagta ttcattaatc cacaaataat tgaagaggta
     3841 ggtgatgaat ggaagtttag cgaaggttgt ttgagcattc ccggaataag agaggatgtg
     3901 atgcgtaaaa aaataattca tctgcgctat caggatgaag aatttaaaac acatgaaaca
     3961 gtttttgaag gattggcagc acgggtactc caacatgagt acgatcatgt tgatggtaaa
     4021 ttatttgttg agaaattatc accttttaaa aagcaattga taaaaggcaa acttcgtgac
     4081 attaccatag gcaaggttga tgttgattac aaaatgcgtt tcccaataaa gaaatagcag
     4141 caatgttgat atttctgatt ggattcatgg gagccggaaa atcatcgcag gcaaagaaat
     4201 tagctgcaag attaaatttt gaaagcattg atctggatga actcattgaa aaaacagtta
     4261 agcaatcaat ttcagaaatt tttaagataa gcggagagca acattttcgt aaaattgaga
     4321 gtgaaacttt aaagacatta tctgaaaaac aaaatgcagt gattgcaacg ggcggtggca
     4381 caccatgctt ttttgataat atgcaatgga tgaatgaaaa aggcattact gtttatctga
     4441 aaatacatcc cggcacagtt tatcaccgca ttgcaccttc aaaatctaaa cgacctttga
     4501 ttagccacat gaacgatgca gatttgatgg agtacattat ggagacgatg attgaaaggg
     4561 cacctttcta taatcaggca aaaattcaga tgtcgggact aaatcttaaa acagaagatt
     4621 tggtaagtgc acttaagcca tttttgaaag attaaaacaa tagcatttca gattgactat
     4681 ttttttttat gtttaccttt caaaataaaa tattatgaaa acattattat gctttttgct
     4741 gcttccaacc atattggttg cacaaacatt tcaaaaaaat caaagtgaag gcaatgagga
     4801 ttttatcaaa cggattgctc cacattcggc taatgaagtt aaatatcttc tgagtgattc
     4861 ttttaatact tcaacagaaa aaattgttta tgcctacagc atttttgagc atggcaacct
     4921 gataacaaac aacgatagta ctttgaccta ttttttatgc atccttgttc ctacttctgt
     4981 aggagcattg gattacaaca tgcagcagtt tcgtatggtg gcaagctata aaaagaaagc
     5041 aaggattgag agtgcagaga ttattaagtc gggacgggaa aagaaacttc aggtatatat
     5101 tgcagaaatg gtacgaggcc ccggaggtgt gccaagagat gtaaagcgca cttttgttta
     5161 taagcaggaa aaagaaagca gaatgtttac tgatactttc agagaggttt tgggtgacta
     5221 ttaaaaacag ttgtccttaa tatgctgatt tattgctgac gttctttttt tcagattttc
     5281 atagttgtga atggctacat tcacccttat tttcatatcg cttaacaatt tattgctaaa
     5341 ggaaactatt ttataatatt ttcgtattgt atttattaaa ccaccaatga caaaggaaga
     5401 tggcatcgca atttatcccg gtagatttta acccttttga agaagttatt ccggacaatt
     5461 tttttaccac atcagaagag caacgtgaaa tctggacatc cgttcaaata ggaggtgatt
     5521 ctgcatcatt agcctataac gaaagtgtta cactttttct gaaaggactg tgtaatcaca
     5581 agcacctttc agaagcttgc aagaatgttg ttcaaaggca tcagtcactt cgctctactt
     5641 tcaatacttc aggtgaaaca tttcaggtac atgaacatgt caatcttgaa attccatata
     5701 ttgatttaac cgacttaagt ccgtcagata aagatgcacg gatacttcat ttttcgcagc
     5761 gtgaagtgtc catacctttc gatctattga atgaacctgt aataagattt gccattttta
     5821 aaatttcaaa ttcagaaacg gcattggtca tcacagcaca tcacattgtc tgtgatggat
     5881 ggagtattgg tatcatcatg caggatatca gtaaatttta ttctgcttta gcaaataata
     5941 cgcaacctga tcttgaacct gttgtaccct tcagcactta tgtaaagcgt caggaagaaa
     6001 ggaaattgaa tggggaatat aaacgtacag aagattattg gctcaattta tattccacag
     6061 atgttcctac cgttgatctt ccggttaaca aagctcgccc tgctgcacgt accttcaatg
     6121 caaagcgatt tgatataact attgatgccg gtttggttag tgatttaaga aaacttggtg
     6181 caaaaaatgg aagcagttat gtaaatacgt tgtttgctgc atttgaagtg tttctttttc
     6241 gtttaacatc caatccttca ttggtattag gacttcctgc tgcaggacag tcagcgtccg
     6301 gaatgtatgc tatggttggt cattgtgtta atcttttacc aattaaaagt cttgttgatg
     6361 agaacatcac tttcggtgaa tatttgaaag ccagaaaacc tttgatgttt gatgctttcg
     6421 ataatcagga atttacaatg ggaagtttgt taggaaaact tccgctgcaa cgcgacccgt
     6481 caagaattcc attagtgcct gctgtgttca atgttgactt gggtatgact caaggagttt
     6541 cttttgaagg attgaactat gaatatactt caaatgccag aaagtttgaa aactttgaat
     6601 tgtttgtgaa tgcaaccggt atgggcgata aactcattat tgaatgtact tacaataccg
     6661 acctttttga agatgagatg atgcaattga gaatgcagga gtttgtagtg cttcttaatg
     6721 gtttggttga gtatgttgat agtgcaataa aaaatttgcc attactgact gacgatgaaa
     6781 aaagaaagtt gtttatcgaa tggccagttg taaaagagaa taaacttagc aatgagtgca
     6841 ttcatacttt aattgaaaag caagcatcgg caaatcctga tacaaatgca atttggttta
     6901 atgggcaatc aataacttac ggacaactaa atcaacgagc caatcagtta gcacattatt
     6961 taatacagtc tggtgttaaa caggaaacat tagttggaat ttgtcacgac aggacagacg
     7021 atttaattgt tgccatgctt gcagtattaa aagccggtgg tgcctacgtg cctttagatc
     7081 caaattatcc tgcagataga attgcctata tcttgagcga tgcaaaagca cctctcttga
     7141 tttcatcgtc aaaatacata gatcagttag gtgaaacagg aacaaagtta gtttgtttag
     7201 ataaagaagt aactgaaatt tctaaagaga aaataacaaa tcctgatgtt gaaattactc
     7261 ctgatcagtt agcatatatt atctacacat ccggatcaac aggaaaacct aaaggtgtag
     7321 ctatcgaaca ccgcaatgca gttgccttaa ttgattgggc acagacagtt tattcttcag
     7381 aggaacttgc aggtgtgctt gcttctacat ctatctgttt tgacttatcc attttcgaaa
     7441 tattttttac attaagcaac ggtggacgca ttgttttagt taaagatgcc atggctttaa
     7501 atgccttacc tgaaaatgca ggtgttacat tggttaacac agtgccttct gcaattgcag
     7561 aactactcaa acttaaagac ggagttcctt caagcgttaa aacaataaat cttgcaggtg
     7621 agcctttgcc tgttgaattg gttcagaaaa tttattcgca aactactgtc aagaaagttt
     7681 atgatttata tggcccttct gaagatacta cctacagtac atttacactc agaaaacccg
     7741 atggcccata tacaatagga aaagctatca ccaattcgca ggtttatata ttagataaaa
     7801 acagacgacc tgttcctgtt ggtattgcag gagaacttta tatggctggt gatggtcttg
     7861 ctcgcggtta tctctataaa cctgaaatga ctgaagagcg ttttgtggct aatccgtttg
     7921 ttctcggaca aaggatgtac aaaaccggtg accttgggaa gtttatgtct gatggaaata
     7981 ttgaattttt aggccgaata gataatcagg taaaaattcg cggatttaga attgaacttg
     8041 gtgaaataga atcaatgttg cgattgcgaa aagagataaa ggaacttgta gtaatggcac
     8101 gtgaagatca accaggaaat aaaattttag ttgcatacct ttctactaat gcaggaaagc
     8161 agattgatgt caatgaacta cggcaatatc tgagaacaga acttcccgaa tacatgatac
     8221 catcggtatt tatgattctt gaatatattc cgttgacacc taacggaaaa attgacagaa
     8281 gagctttgcc tgttcccgaa gtggaagtta aagcagaaag tgcaaacttt gaagaacctc
     8341 acaacccttt agaaagtttg cttgtaggta tttggagtga agtgctggga attgataaaa
     8401 ttggtattca cgataatttt ttcgagttgg gaggacactc acttcttgga attagaatga
     8461 tgggagctat agagcagaag acaggagtta aattggatta tccaacatta ttccgtgctt
     8521 caaccatttc gcaattaact aaggtgatga atgcagaaga gttgaacatt gactggccaa
     8581 tagtggttcc attgcagcct aatggaaaag aaagaccgtt gttctttgtt cacatgcaca
     8641 atggaaacat tcagcgatgg agagtcttat taaaacattt ggataaggac caacctattt
     8701 atgctattca accccgcgga cttgatgaaa aatatgacta tcattattct gttgaggcaa
     8761 tggcttcttt ctatattgat gagattaaaa agattcaacc tgtaggtcca taccgaattg
     8821 cgggattatg ttttggtgga acaactgcat ttgaaatggc tcgtcagtta aaaacaaaag
     8881 gtgaaactac cgaacttgtt tttatgatta acaattatgc accaccatcc aatccaatgc
     8941 aatataaaat ccgcgaaaca tgggatgaat ttaaaggcat gactttggaa gataaacttg
     9001 aatttgcact tgaaaaaact aaaaatgcag gtaaaaaagt tttaagcaaa ctgactaagg
     9061 tttttgattc cggcagcact acagcctctg aagttaaagt aaatactaag cctgacatac
     9121 gttgggtaaa ttcagtagcc ttgctcaatt atcagcccga aggtgaatat tctgaaaaag
     9181 taattttaat caaaaccggt gaagaagttg ccaaacatta tgacgagaca ttgggatgga
     9241 aacgacttat aagtggcgag atagaaatgc atcagattac cggaagcgat aacgatacaa
     9301 tcattaccca taaagaatat tatacgcagt tagcgcagta tttaaatcag gcaataaacc
     9361 aaattaaata accgctgaga tggatttgca gttaactccg gaacaggttt tttgcaatga
     9421 attattaatt caggacaatg attgcttagt ttttattttc tgttcagact catttatagc
     9481 agtagaaagt gaattgaaaa aacttttaag caatgatgaa acagaaagac ttcttaaatt
     9541 taagtttgaa aaagacagac agacctattg cattgcacat ggattgaaaa gagttttact
     9601 tgctgcattg ctcaatacaa aagaacaagt agagatttgt tcacatagca atgggaaacc
     9661 atttattaat catcaaaaac ataatatgcc gctacatttt agtttgagtc attcagaaaa
     9721 aatgatagca ctggcatttc agaaaaatag cccgttgggt gtagatatag aaaaaattca
     9781 accggcagat aaatatggaa atgtgattca gcattatttt acttcggatg agcaaaagct
     9841 aatttcccgg tcagacaatg ataccttttt taaaatctgg actcagaagg aagccattgg
     9901 aaaagccaca ggtcagggta tagccaattt acctgaaatt gtcattccgg atgactatga
     9961 gttaagtact gcatctttag gcggatatat tttaagtaca tgttttttca gaggtaatga
    10021 gattagtttt aagacaattc atgcacagga tgttttaaat cagattaata tactatcttt
    10081 gcgcccataa ttaacattat aatcaaccgg ataatttttt gaaaaacact gataattccg
    10141 gttcttccat cttcaaatga acaaaacttc tttccatgag ctttcgttaa gtcatgaaat
    10201 gctccaggct attgacgaaa tgggtttcac tgaggcttcg cccatacaag cacaagccat
    10261 acctgtattg atgcagggtc aggacgtaat agggcaggcg caaacaggta ccggaaaaac
    10321 agcagctttt ggtattcctt tactcgaaat gctcgaccct aaagttaagg caacttcagc
    10381 attggtaatg tgcccaactc gcgagttagc cgtacaggtc gcacaagaaa ttaaaaaatt
    10441 agccaagtat aaaaagggta ttgctgtgct tgccgtttat ggtggcgagc aaattcaaaa
    10501 gcagataaat gcattaaaac gccctgtaca tgtaattgtt ggtacaccgg gcagaattat
    10561 ggatcatttg gacagaagaa ctataaacct gtctaaaatt cagatggtag ttcttgatga
    10621 ggctgatgaa atgttgaata tgggtttcag agaagatatg gaagcaattt tgcaacatgc
    10681 ccctgacgaa agacagacgg tgttgttttc tgcaactatg cctaaaccta ttcttgaaat
    10741 caccaaacgt tttcaaacca atcctaagtt agttaaggtc accaagcagg agcttactgt
    10801 atcatcaatt gaacagattt attacgatat tccatcgtca cagaaagctg atgtaattac
    10861 acaattagta gagttacaca atttaaaact aatgctagta ttctgtaata ccaaacgtaa
    10921 aactgatgag gtaaccgagc agtttcagca attgggttat aaggcagagg ctattcatgg
    10981 tgacttgcga caaaatcaaa gaaataatgt tttggcacga ttcaagaaag gtgatgtgaa
    11041 tatgcttgtg gcaacagatg tagctgcacg aggtattgat gttgagaatg ttgatgctgt
    11101 atttaatttc gatcttccac tcgatgaaga aaattatgtg catcgtatcg gcagaacagg
    11161 ccgtgccggt cgttcgggta aagctttcag ttttgtttcc gggcgaaatg atatggctaa
    11221 aatccgtgag attgaagatt attcaaaagt gaggatagaa agacatcagc tgccatcagg
    11281 aaaggagcgt atagagttgg ctaaaattaa attggcagca agattgaaag ttcttattga
    11341 ggaaggcgat atggagcaat atgacaacat gtttgcaact ttcaagcagg aaggattaac
    11401 attacatcaa cttgcagcgg cattgcttaa aatgcatttt gcacctatgt tgcaacaaag
    11461 tcaaaataag tcaagcagta aatcatcggg tagagaagaa cgatccggca gagaggaaag
    11521 atccggcaga ggtaatcgtg atagcggtgg tggcaaaggc aaacgcggta aacacagtgg
    11581 cgaagccgat atgattcgtt tatttttaag cgttggcaaa aaagataaag taagtaaagg
    11641 tgatattgtt ggcgcagttt ccggtttaag caatatgagt agtagcaata ttggtgttat
    11701 tgatatttac gataagtttt ctttcgttga agtcaatgga gagtttttaa atgatgtgct
    11761 ttccaacgtt aacggcaata aaataaaagg ccgtaaagta aatgttgaaa ttgctaaaga
    11821 ctaatttaat ctactgtgta ggccagtaga aaatcttttt ctcttctgta acaaatttta
    11881 atgatttctt cttgattgtg ttgtatcagc acagtagcca tacctaaatt taacaattca
    11941 aaaggttgaa tgtgtatgca gtttttaaag tctattcctg a
//
LOCUS       DAORQT010000029        11927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_621_length_11927_cov_8.547338, whole genome shotgun sequence.
ACCESSION   DAORQT010000029 DAORQT010000000
VERSION     DAORQT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_11927_cov_8.547338"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1393)
                     /locus_tag="PK134_02150"
     CDS             complement(161..1393)
                     /locus_tag="PK134_02150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB37687.1"
                     /translation="MKVPFAPPRMDQRVIDEVADTLLSGWITTGPKTKKFEKLLTAYC
                     NVPNVLALNSATAGLEIMLRWFGVKEGDEVILPAYTYSATANVVIHCGAKPVFVDVGD
                     DFNILPSEIENAINKNTKVIMPVDFGGLPCDFQKVNNIFQSENIKNKFVPATDVQQQL
                     GRILVLSDAAHSFGATVNNIKTGALTDVSVFSFHAVKNLTTGEGGAIALNLPKPFDNQ
                     SIYDYLCIYTLHGQNKDALAKMQKGNWRYDIIEAGFKCNMTDIMASIGLVELERYAET
                     LERRKQIVANYNEAFAKYPWAQLPEFKSNWRESSYHLYPIRIKNCTEKQRDAIMQKMF
                     DADIAVNVHFIPVPMFSFYTKLGYSLQHYPKAKDNYEREISLPLFYNLTEEQQQYVID
                     NLIKAVNETLVHRPAINS"
     gene            complement(1416..2570)
                     /locus_tag="PK134_02155"
     CDS             complement(1416..2570)
                     /locus_tag="PK134_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB37688.1"
                     /translation="MLLFFVVQWLTGKYQIKIQNPLLWLPLCYFLLHCIGMLYTQSQY
                     TGWIELSAKSSFLIFPLLFSSNAISRDIIKTFKLFFIAGSGIICLYNLLLSFNQYIQH
                     GNMSAFYYVAFSKGMHVQYLTIYLNLAILFILEILFDASSQTKPIKKAYWVLLVLFFL
                     VNITLLASRLATVVSYATILFYTVVRIISSPNYLRYSILAALFAASVLIIDYFAFKKI
                     NRFEQVEIFLKENTNYVDFSKTEYNSTTLRIPLWINAYEVIQRNPLFGVGTGDVKSSL
                     DSMYLKNKFTYAHQNHFNPHNQYLQTGVAFGLTGISFLMLMMLIPLYYAIRNRHYIFI
                     AFQCIFLLNMLTESILERQAGIILFCLFYAIFSSEYVAAALKTVPEQNNT"
     gene            2820..>3155
                     /locus_tag="PK134_02160"
     CDS             2820..>3155
                     /locus_tag="PK134_02160"
                     /inference="COORDINATES: protein motif:HMM:NF023145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HRB37689.1"
                     /translation="MKKLIFTSLFFLGFLTFTYAQKVNEKVQINSKGAWYPGKILKIN
                     ADDHTYYVSYDGWDEGWNEWVTIDRLKDFAKEAPAQPLTKFKVGDRVEVEYGMVPEPA
                     TVIEVGENKY"
     assembly_gap    3156..3187
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3321..4517
                     /locus_tag="PK134_02165"
     CDS             3321..4517
                     /locus_tag="PK134_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37690.1"
                     /translation="MIKEIRDKYLREFKEETYSEFLAYYDNLYNRKVEFRIAESPVFI
                     PKKLKDLLVEAGNEILSQSLTPEYLLKSEQAIPHGLKVPNEHKHPHFIAIDFAICKDD
                     NGNYLPQLIELQGFASLFFWQDLLPRTYAKYFQIPEGFSHFFDGWNTEKHRALMRKVI
                     LGNHKPENVILLEIEPEKQKTWIDFWETKTYTGVEPVCISKVIKEGKKLFYDLNGVKT
                     PIYRIYNRIIFDELLQRKDLIKDFNLTDDVDVEWAGHPNWFFRISKYTMPFIKSRFAS
                     ECYFLSDVDTSKLSLADFVLKPLFSFAGSGVLIDVTSSDIESIPLEKRNEFLIQRKVN
                     YVPCMETPDGAAKVEVRLLYIWDDAETKPQLVLNLTRLSKGAMIGVSHNKNKTWVGGS
                     SSLMEI"
     gene            complement(4514..7942)
                     /locus_tag="PK134_02170"
     CDS             complement(4514..7942)
                     /locus_tag="PK134_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRB37691.1"
                     /translation="MPDVNTTFINLNIKHLSTNKTDIETIKQYPFTEQQYIKLPDNIS
                     ELGKIVYRGVFTGFYNDFVSYGNITTAIGNLSTDVNLKFKNKANESTYSGKLTADNFD
                     IGKFWRLGNAVGTISFNAQLNGTGFTSEKVNARLTGLVSHVNLNGYDYSNINLDADVA
                     RKLFNGTLNMNDRNAQVSFKGSIDFREKLPLFDFNAIVKNAALTKLNLLKRDTSALLS
                     TEANLNMRGFNFDEFEGKLQLKNISYSEEYKSAFVEKILIQSGMENNNRNLNVNSDLL
                     DLNINGQYILSKIVYSFGHLLNCHLPPFMLKLSPDVEKTNTIFSFSGNIKDVNPVLNI
                     FTPGLSVSNQTEFSGRLNRQHCEIEVDATSPWINYKNFENKNIKLTSNNKNNLLNIDL
                     SASQVKISDSLILKNILLDAETQKSKSSIVLTVANDSLSTSKMRLATEQTYTNGATRF
                     HLLESLLQFKGEQWQVDPANEILFDSASISFDKVLFTNKSQSLMLQGKVGNNKEDVLS
                     VNLFKFKVPLINDLLAMYDIKLGGAASGNLNFSSLYQRAIITGEMRVDSFAFNDEPLG
                     NAIVNFDWLTDQKKLHIEGAIEQNNRNSAVVKGDYIFQPKNDELSFNIKVDKLSITPL
                     ERYLNGFASKIVGLATGELNLTGLAKAPNLTGKARLQRASMVIDYLNTRYSVLPEQDI
                     IFDEKYIAFNKVGISDDQNHKGIVDGKIFHNHFNDFTFSLRVDANKLQCLNTNASQNE
                     LFYGKAFATGYAKIIGTLNLMKLDVVARSESGTQIFIPLSNPEEVTQSNYINFINVND
                     TAAKKFVVTNKVDLSGIQMDLQLEATPEAEIQLIFDSKVGDVMRGNGSGILRMQISQA
                     GEFSMYGDYNILAGDYLFTLQNIVNKRFKIQSGLIQWAGSPYDATININAKYNLRAST
                     FDLIPDSSNRSRVPVEVLLQLKNNLMSPDVIFDVNVPNATPAVRTSLSSILGNEREMN
                     RQVFSLLVLNRFSPPEGKNTSEESGSGNAVGQNLSEFVSQQLSNWASQLSDKFNIGVN
                     YRPGDALNRDEFDVSISTELFRNRVAVESNVGVANNSNQTSSIIGDFQVEFKISKDGN
                     LRAKAFNKTNTNNLINNLNSQYTQGIGVFYRKEFNTVSDLVESFRKRKKAAAPN"
     gene            complement(7979..>8378)
                     /locus_tag="PK134_02175"
     CDS             complement(7979..>8378)
                     /locus_tag="PK134_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37692.1"
                     /translation="TLYNIKPTDSSFSFYGSKISLKEKSGFKINSFSTYAAFYNDSMS
                     FNKILLRTPYSSIDAGHYSMSYNSMTDFEDYINKVMMKGEFNESTISSNDIQYFAEEL
                     FGLDKRVEFSGTARGTVSNLKTKKLKFAYG"
     assembly_gap    8379..8411
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8412..8912)
                     /locus_tag="PK134_02180"
     CDS             complement(<8412..8912)
                     /locus_tag="PK134_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37693.1"
                     /translation="MLLLLFLFSVSLRFQKVQSWVVDRVTGYLSSELNTTVHIGGVRF
                     VLIKSVYLDDVFIADKQQDTLLYAKHLTATVAFQSLFIRNKKLILDEVQLDKARIALS
                     KSKSTRDMNFDFIIDYFSSPSKDTTSSPFYFAVNRVILSNIHFTYRDFKWDDKTPCID
                     FEDIDVS"
     gene            9158..10195
                     /gene="tsaD"
                     /locus_tag="PK134_02185"
     CDS             9158..10195
                     /gene="tsaD"
                     /locus_tag="PK134_02185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRB37694.1"
                     /translation="MTVTDKKDIIILGIESSCDETSAAVSRNTQILSNVIANQTVHQK
                     YGGVVPELASRAHQSNIVPVVDQALREAAINISDVDAIAFTRGPGLIGSLMVGVSFAK
                     SAAAALNKPLIEVNHMQAHVLANFLSLEGQEGKTPSFPFLCLTVSGGHTQIVRVTSPQ
                     QMEIIGQTLDDAAGEAFDKIAKVFELPYPGGPLIDRYAQSGNPKAFNFPVANLPGLNY
                     SFSGLKTSVLYFLKKQLAQNPAFIAENRDDLCASVQYTIVKTLILKLKKAALETGISN
                     IAIAGGVSANSGLRKAVTEEAVKNNWQVHIPPLSLCTDNGGMICLAAYFKYLAADFST
                     LDVAPSARFGF"
     gene            10304..11188
                     /locus_tag="PK134_02190"
     CDS             10304..11188
                     /locus_tag="PK134_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37695.1"
                     /translation="MENQSKGNNRIWIVLFAASALFNVYQWRNHTSTTEVFEVTKDSL
                     ITARVDVEKELGATIEELNKYKGINDNLDSLLTEANHKIDEQKERIQKLIRSEGNSAA
                     LNKKLMAELESLKKLRDEYLDKIDTLMVENQKLKDDNAQLSGTVETLSKNLETTVNTA
                     SVLKSEYFKVSTFKKKGNGKFVSTALSKRTNKMDVCFSVLENKIAKAGEKNVYLRIIE
                     PGGKVMGNRSAGSTSFKMDSGEEVMCTVVSSLRYENATVDNCLSYEENDRIFTPGTYL
                     IEVYIDGNLSGASSYVLR"
     gene            complement(11559..>11927)
                     /locus_tag="PK134_02195"
     CDS             complement(11559..>11927)
                     /locus_tag="PK134_02195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRB37696.1"
                     /translation="FIRRVNGFKPAQVFAIDLDGEAIGAIGLHPQTDIFLKNAELGYW
                     LAEKYWGKGFVSIAIKKIVEYGFSSFEIDRIFARPFGSNKASQRVLEKTGFVLEARLE
                     KIIYKNNRYEDELIYAIRRK"
BASE COUNT         3808 a         2121 c         2156 g         3777 t
ORIGIN      
        1 cccgatagct atcgggaggt taaagtagga gatgaatctt ttgaaaacct gcaatgtctt
       61 gccctaaaat agagttacac aaaaaaagtg taaaacattg gcaaaaaaat cattcaaaaa
      121 gcacagaata ttttaatgaa gaagaaaagc acaaaatcat tcaggagtta attgcaggcc
      181 gatgtactag cgtctcattc acagccttta tcaaattgtc aataacatat tgttgttgtt
      241 cttcggtgag gttgtaaaaa agcggtaaag aaatttctct ttcatagttg tcctttgctt
      301 tgggataatg ttgaagacta taacctaatt tggtataaaa cgaaaacata ggaacaggaa
      361 taaaatgtac attcactgct atatcagcat caaacatttt ttgcataatg gcatcacgtt
      421 gtttttctgt acagtttttt attctgatag gatagaggtg ataggagctt tcacgccagt
      481 ttgatttaaa ttcgggcaat tgcgcccatg gatattttgc aaatgcctca ttgtagtttg
      541 caacaatttg ttttctgcgt tctaatgttt ctgcatagcg ttcaagttct actagaccaa
      601 tggacgccat aatatctgtc atattacatt taaatccggc ttcaataata tcgtaacgcc
      661 aattgccttt ttgcattttt gccagtgcat ctttattctg tccatgcagg gtgtaaatgc
      721 acagatagtc atatatgctt tgattatcga aaggctttgg caggtttaat gcaatggcac
      781 caccttcacc tgtagttagg ttttttacag catgaaaaga aaaaacagaa acatcggtca
      841 gcgctccagt tttaatattg ttgacagtag cgccaaaaga atgtgcagca tcagacaaaa
      901 ctaaaatgcg tcctaattgc tgctgcacat cggttgcagg cacaaattta tttttgatat
      961 tttctgattg aaatatatta tttacttttt gaaaatcgca gggcagccca ccaaaatcaa
     1021 caggcataat cacctttgta tttttgttga tggcgttttc aatttcagag ggcagaatat
     1081 taaaatcatc acccacatca acaaacacag gttttgcacc acaatgaatc actacgttgg
     1141 cagtagcaga gtaggtgtat gccggtaaaa ttacttcatc accttctttt acaccaaacc
     1201 agcgaagcat tatttcaaga cctgcagtag cagaattcag ggcaagcaca ttaggcacat
     1261 tgcagtatgc tgtaagtagt ttttcaaact ttttagtctt aggccctgtt gttatccagc
     1321 ccgaaagaag tgtgtctgca acttcatcaa tgacacgttg gtccatgcga ggtggtgcaa
     1381 atggtacttt cataaattga aattaggttg taaaattacg tattgttttg ttcgggtact
     1441 gtctttaatg ctgcggcaac atattcagag ctgaaaatag catagaaaag acagaataaa
     1501 ataatgcccg cctgcctttc taagattgac tctgtgagca tattcaataa aaaaatacat
     1561 tgaaatgcaa tgaaaatata atgtctgttc ctgattgcat aatagagcgg aataagcatc
     1621 attaacatta aaaaagaaat tcctgtcagt ccaaaagcaa ctcccgtttg tagatactga
     1681 ttgtgtgggt tgaagtgatt ctgatgggca taagtaaatt tatttttcag atacatagaa
     1741 tctaaacttg acttaacatc gcccgtgcct acaccaaata aaggattgcg ctgaatgact
     1801 tcataagcat ttatccaaag tggaattctt aacgtggtgc tgttatattc agtttttgaa
     1861 aagtcaacat agtttgtgtt ttcttttaaa aatatttcga cctgctcgaa gcggttaatc
     1921 ttcttaaatg caaaataatc tataatcagc acagaagctg caaacaatgc tgcaagaatg
     1981 gagtatcgta ggtaatttgg tgatgaaata attctcacaa cggtataaaa caaaattgtt
     2041 gcataagata ctacggtggc aagtctgctg gcaagcaagg taatattgac taaaaagaac
     2101 aagaccaata atacccaata agcttttttg attggttttg tttgagagga agcgtcaaaa
     2161 agtatttcta agataaatag tattgcaaga tttaaataaa ttgtcagata ctgcacatgc
     2221 atacctttgg aaaacgcgac ataataaaat gcactcatgt tgccatgctg aatatattga
     2281 ttgaatgaca gcagcagatt atacaggcat attattccgc ttcctgctat aaaaaataac
     2341 ttaaaggtct taattatatc acgcgaaatg gcattggacg agaagagtag tggaaaaata
     2401 aggaaagaac tttttgccga taattcaatc caaccggtat attgcgattg tgtgtaaagc
     2461 atgcctatac aatgcagcag aaaataacag agaggcagcc acaacaaagg gttttgaatt
     2521 ttaatctgat attttcctgt cagccattgt acaacaaaaa ataaaagcaa caggccaagc
     2581 gcataattta tccaaggctg gtcaaatggt aacaaaaacc ctgtaagcca gaataagtaa
     2641 ttaaagatta aagcccgttt ttcctgaacc attttacagt atgcaaatca aacaaaattt
     2701 tgattcggac tggtaggatt ctatacacga agataaattt tttatgaggt ttataaatta
     2761 gcggtagaat ggcctcctta acctgaataa aatactatat ttggggaaaa ttaaataaaa
     2821 tgaaaaaact aatattcaca agtttgttct ttttagggtt tttaactttt acttatgcgc
     2881 aaaaagttaa cgagaaagta caaataaatt caaaaggcgc atggtatccc ggtaaaattt
     2941 tgaaaattaa tgcagatgat cacacctatt atgtttctta tgatggttgg gatgaaggtt
     3001 ggaacgagtg ggttacgatt gatcgtttaa aagattttgc aaaagaagca cctgcacaac
     3061 cattgacaaa atttaaggtt ggagataggg ttgaagtgga atatggtatg gttcctgaac
     3121 ctgcaactgt aattgaagtt ggagaaaata aatatnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnata tcatattaaa tatgataaaa cagtttttaa agataggtgg gttacagaaa
     3241 gagagataaa aaaactttaa acggaaattt attttacttg ggtgatttag aaataagtca
     3301 ccctttttta tttttgacac atgattaaag aaattagaga taaatattta agagaattta
     3361 aggaagaaac atactcggaa tttttagctt attatgataa cctttacaat agaaaagtag
     3421 aattccgaat tgcagagtcg ccagttttta ttccaaaaaa attaaaagat ttgctagttg
     3481 aagccggtaa tgaaatatta tctcaatcac tgactcctga atatctttta aaaagtgaac
     3541 aagctattcc gcatggatta aaagttccga atgaacataa gcatccacat tttattgcaa
     3601 ttgattttgc catctgcaaa gatgataatg gaaattattt gccgcaattg atagaactgc
     3661 aagggtttgc ctctcttttt ttctggcagg atttgttgcc gcgcacttat gccaaatatt
     3721 ttcagatacc tgaaggtttc agtcattttt ttgatggatg gaatactgaa aagcatcgtg
     3781 cattgatgcg aaaagttatt cttggaaatc ataaacccga gaatgttatc ttgcttgaaa
     3841 tagaacccga aaaacaaaaa acatggattg acttttggga aacaaaaaca tatacaggag
     3901 tagaaccggt ttgtatttcg aaggtgatta aagagggaaa aaaattgttt tatgatttga
     3961 atggggtaaa aactcccatt tacagaattt ataacagaat tatttttgac gagttgctgc
     4021 aacgaaaaga cctgattaaa gatttcaatc tgactgatga tgtagatgtg gaatgggcag
     4081 gacatccaaa ctggtttttc agaatcagta aatacaccat gccatttatt aaaagcagat
     4141 ttgcttcgga atgttatttt ttaagtgatg ttgacacatc aaaattaagc cttgctgatt
     4201 ttgtgttaaa gccgctattt tcctttgcag gtagtggggt gttgatagat gtaacatcgt
     4261 cagacattga aagcattcca ttggaaaaaa gaaatgaatt tttaatacag cgaaaagtaa
     4321 attatgtgcc ttgcatggaa acacccgatg gagctgcaaa ggtggaagtt cgtttgcttt
     4381 atatctggga tgatgctgaa accaaaccgc agttagtgct taatcttaca cgattaagta
     4441 aaggtgcaat gattggtgta agccacaata agaacaaaac ctgggtagga ggaagcagct
     4501 cgctgatgga aatttagttt ggtgcagcag ctttttttct ttttctgaac gattcaacta
     4561 agtctgaaac agtattaaat tcttttctgt aaaaaacacc aattccctga gtgtattgtg
     4621 agttaaggtt atttatcaaa ttgttggtgt tggttttatt aaacgctttt gcacgaagat
     4681 tgccgtcttt gctgatttta aattcaacct gaaagtcgcc aataatactt gaagtctgat
     4741 tgctgttgtt ggcaacacca acattacttt caacagcaac tctgtttctg aacaattctg
     4801 ttgaaataga tacatcaaac tcatctctgt taagtgcatc gcccggacga tagttaactc
     4861 cgatattaaa tttatcactc aactgcgatg cccaattcga tagctgctgc gaaacaaatt
     4921 cactcaaatt ctggccaact gcattgccgg aaccactttc ttcagaggtg ttttttcctt
     4981 ccggtggcga aaaacggttt aacacaagca gactgaaaac ctgcctgttc atttcgcgct
     5041 cattacctaa aatactactc agacttgttc gcacagcagg agtggcattt ggcacattta
     5101 cgtcaaaaat aacatcagga ctcattaagt tgttttttaa ctgcagcaat acttccaccg
     5161 gtactctgct gcggtttgag gagtcaggaa tgagatcaaa cgttgatgca cgaaggttgt
     5221 attttgcatt gatattgatg gttgcatcgt aaggcgagcc tgcccattgt atcagcccgc
     5281 tctgaatttt aaatcttttg ttaactatat tttgaagtgt aaacaaataa tcacctgcaa
     5341 gaatattgta atcaccatac atggaaaatt cccctgcctg cgaaatctgc atccttaaaa
     5401 ttcctgatcc attaccacgc atcacatcac caacttttga gtcaaatatc agctgaatct
     5461 ctgcctcagg cgttgcttca agctgtaaat ccatttgtat gcctgacaaa tcaactttgt
     5521 tagttaccac aaactttttt gcagcagtat cattcacatt gataaagttg atataattac
     5581 tctgtgtcac ttcttcaggg tttgaaagag gaatgaatat ttgtgtgccc gattcagaac
     5641 gtgccactac atccaatttc atcaggttta acgtgccgat tatttttgca taacctgttg
     5701 caaaagcttt gccatagaac aattcgtttt gagaagcatt cgtgttaaga cattgaagtt
     5761 tatttgcatc aactctaagg ctgaaagtga aatcattaaa atgattatga aatatttttc
     5821 catcaacaat gcctttgtga ttctgatcat cagaaattcc aactttgtta aatgcaatat
     5881 atttttcatc aaaaataata tcctgctccg gcaacacaga atagcgtgtg ttcagatagt
     5941 caattaccat agatgcacgt tgtaatcggg ccttgccggt taggttaggt gcttttgcca
     6001 atcctgtaag atttaactct cctgttgcaa gtcctacaat tttacttgca aaaccattaa
     6061 ggtatctttc taaaggtgtt atgctaagtt tgtcaacttt aatattaaaa ctcagctcat
     6121 cattttttgg ttgaaaaata tagtcacctt ttacaacagc actgtttcta ttgttttgtt
     6181 caatggcacc ttcaatatgc aatttctttt ggtctgtcag ccaatcaaaa ttcacgatgg
     6241 catttcccaa tggttcgtca ttaaacgcaa aactatccac tcgcatttca ccggtgatga
     6301 ttgctcgttg atagagtgaa ctgaaattca aattaccact tgcagcacca cctaacttga
     6361 tatcatacat agccagtaag tcatttatta aaggaacctt aaatttgaat aagttcacac
     6421 tcaacacatc ttctttattg tttcctactt tgccctgcaa cataagcgac tgacttttgt
     6481 tggtgaacaa caccttgtca aatgaaatag atgctgagtc gaataatatt tcgtttgccg
     6541 gatcaacctg ccattgttcg cctttgaact gaagcaaaga ctcaagcaaa tgaaaacgtg
     6601 tcgctccatt tgtgtaagtc tgctctgttg ccagtctcat ttttgaggta gagagtgaat
     6661 cgttggcaac tgtaagtaca atggatgatt tgcttttttg tgtctctgca tcaagtaaaa
     6721 tatttttcag aattaaactg tcagaaattt taacctgaga ggcagataaa tcaatattta
     6781 ataaattgtt cttattgttt gatgtgagtt taatgttttt attttcgaaa tttttgtaat
     6841 taatccaggg tgatgttgca tccacttcta tttcgcaatg ctgacgattc agccttcctg
     6901 aaaactcagt ttgattactt acagacaatc ccggagtaaa gatgttcaac acaggattta
     6961 catcttttat atttccactg aatgaaaaaa tagtattagt tttttctaca tcaggtgata
     7021 atttaagcat gaatggtggc agatggcagt taagtagatg accgaatgaa taaacaattt
     7081 tagataatat atactgacca ttaatattca aatccagtaa atcggaattc acattcaggt
     7141 ttctgttatt attctccatg cccgactgaa tcaatatttt ttctacaaaa gcagatttat
     7201 attcttctga ataactaata tttttaagtt gcagttttcc ctcaaactca tcaaaattaa
     7261 acccgcgcat gttcagattt gcttcagtag aaagtagggc agatgtatct ctttttagca
     7321 gattaagttt agtcaatgcg gcatttttaa ctatagcatt aaaatcaaac aaaggcagtt
     7381 tctctctgaa atcaatactt cctttaaatg aaacctgtgc atttctgtca ttcatgttta
     7441 atgttccgtt aaaaagtttc cgggccacat cagcatctaa gttgatgttt gaataatcat
     7501 agccatttaa attaacatga cttaccaaac ctgtcagacg tgcattaact ttctctgatg
     7561 taaatcctgt accatttagc tgtgcattaa aactgattgt tcctacggca tttcccaatc
     7621 gccagaattt accaatatca aagttgtcgg cagtgagttt accggagtaa gttgactcat
     7681 ttgccttgtt tttgaatttt aaatttacat cggttgacag gttgccgatt gctgttgtaa
     7741 tatttccata cgaaacaaag tcgttataaa atcctgtaaa aacacctctg taaacaatct
     7801 taccaagttc agaaatgtta tcaggcaatt taatatattg ttgctctgta aatggatatt
     7861 gtttaatggt ttcaatatcg gtcttgttgg ttgaaagatg ttttatgttt agatttataa
     7921 aagttgtatt tacatcaggc aaaccattaa ggcttacatc accttcaaat cgtgtgctct
     7981 atccataagc aaatttaagt tttttggttt ttagattgct caccgttcct ctggcagtac
     8041 cactaaactc aacacgttta tctaggccaa aaagttcttc ggcaaagtac tgaatgtcgt
     8101 ttgaagaaat tgtgctttca ttaaactcgc ctttcatcat taccttattg atgtagtctt
     8161 caaagtctgt catactgtta tacgacatac tgtagtgtcc ggcatcaata gaagagtagg
     8221 gtgtgcgcaa cagaatttta ttaaacgaca tgctgtcgtt ataaaaagca gcataagtag
     8281 aaaaagagtt tattttaaat cctgattttt ctttcagaga tattttactt ccataaaatg
     8341 aaaacgagct gtctgttggc tttatattgt aaagtgttnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn ntgacacgtc aatatcttca aaatcaatac aaggtgtttt atcatcccac
     8461 ttaaaatcgc gataagtgaa atgtatattg cttaaaataa ccctattcac ggcaaaataa
     8521 aatggtgatg atgtagtgtc ttttgaagga gaactgaaat aatctataat aaaatcgaag
     8581 ttcatatctc ttgtgctctt cgactttgat aatgctattc gcgctttgtc caactgcact
     8641 tcatcaagaa tcagtttttt attcctgata aacaaggact gaaaagccac tgttgctgta
     8701 aggtgtttgg catacaacag cgtatcttgc tgtttgtcgg caataaacac atcatcaaga
     8761 tatacacttt ttatcaatac aaaccttacc ccgccaatat gtactgttgt atttaattcg
     8821 gatgatagat atcctgttac acgatcaaca acccaacttt gaactttttg aaatcgtaag
     8881 ctgacagaaa acaaaaaaag cagcagcaac aatgtgccaa ttgtaataag taatattttt
     8941 aaaaatttcc tgatgacctg ttgtttttga agcggataaa aatagtgtaa ttatttaagt
     9001 ggattaacca atgttgcctt aaaaataatg ccacactctt gttagtttac cctaaattca
     9061 ttgcattata gatggttaat aagatgtgtt ttgaagcctg tcagacaaac tgatttttcc
     9121 tcttaacttt gctgactgat ttttagtgta aaagaaggtg acggttactg ataaaaaaga
     9181 cataatcata cttggcattg aatcatcctg tgatgaaact tctgctgccg taagtcgcaa
     9241 tacacagatt ttatcaaatg ttattgccaa ccaaaccgta catcagaaat atggcggtgt
     9301 ggttcctgaa ttagccagca gggcacatca gtcaaacatt gttcctgtag tggatcaggc
     9361 attacgagaa gcggcaataa atatcagcga tgttgatgcc attgccttta ctcgtggacc
     9421 aggacttatt ggctcactaa tggttggtgt ttcttttgcc aagtctgctg ctgctgcatt
     9481 aaacaagccg ctcatagaag tcaaccacat gcaggcacat gttcttgcta actttttaag
     9541 tttagaaggg caggagggca aaactccgtc atttcctttt ttatgcctca cagtttcggg
     9601 tggccataca caaattgtaa gagtaacttc accacagcaa atggaaataa ttgggcagac
     9661 actcgatgat gcagcaggtg aggcatttga taaaattgcc aaagtatttg agttgcccta
     9721 tcccggaggg ccgctcattg acagatatgc acaaagtggc aatccaaaag catttaattt
     9781 tcctgtggca aacctgccgg ggctcaatta ttcctttagt ggactgaaaa cttctgtctt
     9841 gtatttttta aagaaacaac ttgcgcaaaa tcctgctttt attgctgaaa acagagatga
     9901 tttatgtgct tccgtgcaat ataccattgt aaaaacactc atacttaaat taaaaaaggc
     9961 agctttagaa acaggcatca gcaatattgc cattgctggc ggagtatcag caaacagcgg
    10021 actacggaaa gccgtaacag aagaggcagt taaaaataat tggcaggtac atattccgcc
    10081 actttcgctg tgtactgaca atggcggtat gatttgtctt gctgcatatt tcaaatatct
    10141 tgctgctgat ttttcaacac ttgatgtggc cccaagtgcc cgtttcgggt tctgacttta
    10201 tgcccatttg ttgacattgt acctgttcaa aaccttgaca tacaggagtt gtttacttta
    10261 aaatacatat tacctttgtt aattaatcta taaaaatatt atcatggaaa accagagtaa
    10321 agggaataat cgtatttgga ttgttttatt tgctgcttcg gcattgttta acgtatatca
    10381 atggcgcaat cacacatcaa ctacagaggt atttgaggtt actaaagaca gcctcattac
    10441 agcacgtgtt gacgttgaaa aagaattggg tgccaccatc gaagagctaa acaagtacaa
    10501 aggtatcaat gacaatctgg acagtctttt aaccgaggcc aatcataaaa ttgatgaaca
    10561 gaaagagcgc attcagaaat taataagaag cgaaggcaac agcgcagcat taaacaaaaa
    10621 gctgatggca gaattagagt cactcaaaaa actgcgtgat gaatatttgg ataagataga
    10681 taccctgatg gtagaaaatc aaaaactcaa agatgataat gcacaattat caggcactgt
    10741 cgaaacgctt tctaaaaatt tagaaacaac ggttaacact gcatcggttt taaagtcaga
    10801 atattttaaa gtttctactt tcaagaaaaa aggcaatggc aaatttgtgt ccaccgcttt
    10861 atcaaaacgt accaataaaa tggatgtgtg tttttctgtt cttgaaaata aaattgccaa
    10921 agcaggagaa aaaaatgttt atctcagaat cattgagccg ggtggtaagg ttatgggtaa
    10981 ccgctctgcc ggatctacat cttttaaaat ggatagtggt gaagaggtaa tgtgtactgt
    11041 tgtttcatcg ctgcgctacg aaaatgccac cgttgacaat tgccttagtt atgaggagaa
    11101 cgatagaata tttacaccgg gcacctatct gatagaagtg tatattgatg gcaacctcag
    11161 cggggcatca tcttatgtgt tgagataagt ttttgaattc taccaaaggc tttagcctga
    11221 gatagatgat ccctaagcct catatcggac tttagtccac aaataaaaaa tccaccgctg
    11281 ttaaaaacca tcggtggatt ttttttgggg gattggcttt tctatccatc aggctaaagt
    11341 cttatgctaa tatctattgt ccttacacct aatcacctct gtcagccgtg gctaatcaca
    11401 atttaatgcc tgatgttatt ttcacttttt tcaattctga attctgcact ctgaattctg
    11461 aattttcact ttacttcatc acgtttcaca taatcactta atcacccctt gtcagtcgtt
    11521 gttgatcata acccctcact tcatcgcgct tcacttaatc actttctcct gattgcataa
    11581 atcaactcat cttcataacg attgttttta taaatgattt tttcaaggcg ggcttccaaa
    11641 acaaatcctg ttttttccaa tacgcgttgc gaagctttat tgctgccaaa ggggcgtgca
    11701 aaaattctat ctatttcaaa actgctgaaa ccatattcaa ctattttttt aatagcaatg
    11761 gaaacaaatc ctttacccca atatttttca gcaagccaat atccaagctc tgcatttttt
    11821 aaaaaaatgt cagtctgcgg gtgtagtcca atagcaccta ttgcctctcc atctaagtca
    11881 attgcaaata cctgagcagg tttaaaaccg ttaactcttc tgataaa
//
LOCUS       DAORQT010000030        11246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_692_length_11246_cov_9.255920, whole genome shotgun sequence.
ACCESSION   DAORQT010000030 DAORQT010000000
VERSION     DAORQT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_11246_cov_9.255920"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK134_02200"
     CDS             complement(<1..444)
                     /locus_tag="PK134_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37697.1"
                     /translation="MKTFSGLITVLIFYSVQAFSQGSALMKEDAVQVNKFNDGVYFTL
                     TDLQHNTPTVTKDHLIKSYYDQSDFSIGQWATTQNLFYTAKDEHKLKVNRDSLWGYVE
                     NGTPYIYLNDRFHRFSTAGAISVFTESYPNIKAGLAPVVTEARSSS"
     gene            complement(492..2243)
                     /locus_tag="PK134_02205"
     CDS             complement(492..2243)
                     /locus_tag="PK134_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37698.1"
                     /translation="MSTSGTAFNWKVLNRIMFYVKPFRFLFVATVVITVLLAVLAPLR
                     PWLTQQALDIDIAKHDIKGLTRTVAFMFGVLLLQSLLQFVYTTLTNRLGQEVILSLRN
                     NLFKKVSGFGLAYFDKTPLGITITRLVSDMETIADIFSDGLILIISDMLQVVVIIAVM
                     FYIHPQLAAISLSTIPVLLIATRVFQKNIRKTFNDVRIQVAKLNEFVQEHLVAMRIVQ
                     LFNREEEELNKFHEINAKHRDANIRSIWYYSLFFPVVEILSAVSIGLIVWYGGLNVLQ
                     SSISLGLLVAFIQYINQLFRPIRELADKFNTLQMGMVSSERVFKLLDEPFTEVNSGTK
                     SADNIIGKIEFRDVWFAYKNEEWILRGLSFIIPSGQSVAIVGSTGAGKTTIVSLINRL
                     YEIQKGQILIDGVDIRDYDLYSLRNAIGVVLQDVFLFSDSIKNNITLYREQDDIVIEA
                     LLKQTGAWHFVEKLPGQLHYNPGERGGLISTGQRQLLSFARVLAHQPKLIILDEATSS
                     VDSKLEQLLVNATEMLTKNRTSIIIAHRLSTIEKADRIIVLHKGAIAEDGNHQNLLER
                     KGIYAHLHDVQFKQPSA"
     gene            2349..3680
                     /gene="mtaB"
                     /locus_tag="PK134_02210"
     CDS             2349..3680
                     /gene="mtaB"
                     /locus_tag="PK134_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRB37699.1"
                     /translation="MTRESTVAFHTLGCKLNFAETSTIGRQLQAAGYKKVSFSDTADV
                     YVINTCSVTENADRECRQHVNRALAANPEGCVIIIGCYAQLKPDAIATIPGVSLVLGA
                     SEKFNIPHYLQKLKKQTEAQVFSCDIADHHQFTSSYSEGDRTRVFLKVQDGCDYGCTF
                     CTIPLARGASRSNTIENVIENVKQIVDRGAKEIVLTGVNLGDFGKLPGEKKHATEFFQ
                     LVSALDALDADIRFRISSIEPNLLTEEIIELVSCSKKFMPHFHIPLQSGSDKILKLMR
                     RRYLTSHYTSRINKIKKLMPDACIGVDVITGFPGETEEDFLQTYKYLNEADISYLHVF
                     TYSERANTPAIEMKPVVPVAERKRRTNMLRILSEKKLGIFYRSQEGKTAKVLFEHENK
                     NGIMSGYSENYVRVSTPFNNEMVNKVIECKIGATDNDSHEVNCTILESITA"
     gene            3693..4847
                     /locus_tag="PK134_02215"
     CDS             3693..4847
                     /locus_tag="PK134_02215"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRB37700.1"
                     /translation="MYPTLSDALHDLLGINIPLPIQTFGLILALSFFLAAYILILEFK
                     RKEQNGLLKSFTVTTIKGKPASTAELFWSGVVGFLVGFKLLDAILNYSDLVNDPQGFL
                     LSTRGNVIGGILLGAYSVWSKYKEKEKQKLAVPQTVTEVGYPHQFVMNITIAAAVAGL
                     IGAKIFHNLENIDDFLKDPVDALISFSGLTFFGGLICGALAVIWYSNKKLNVPPLVMC
                     DVAAPALMLTYGTGRLGCHFSGDGDWGIVNTLAKPSWFPFPDWMWSYNYPHNVIGEGV
                     PIAGCVGKHCYELIPPVFPTPLYEAIACILLFFVLWKLRTRIVTPGILFFVYLLFTGV
                     ERLAIEQIRVNTKYHIFGKAITQAELIAVACIIVSIVGITLLRRKNSGSS"
     gene            4911..5888
                     /locus_tag="PK134_02220"
     CDS             4911..5888
                     /locus_tag="PK134_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRB37701.1"
                     /translation="MLHKSFFTAIVFLFLSTSLFSQKRIKIPVLDYGSVDEFVLKLKP
                     EQCVNIDTLAEVISREFDRPHFRFRAAFRWITENIAYDLKNQRHPQKANLNANDVFKN
                     KTALAEGYANILNAICAKMNITCLSVNGYLRRNQLPGTKIKKPNHIWSTVKLYDKWYL
                     MDVMMASGYFEKGDKIFTKRFTERYFALHPRQIILTHLPLNGNRQYLDTIVDKNTFLN
                     YPEVYDFFFDNGIYALRPADGVLPVYKGLKKRFYFKSMDSLAIGKIEIRDGKKSEKIQ
                     NTFTQVDSLVYFDYVFESNKKRTLDVFVDGLPLCTYFIVPDKKKKRSEY"
     gene            complement(5905..6780)
                     /gene="ygiD"
                     /locus_tag="PK134_02225"
     CDS             complement(5905..6780)
                     /gene="ygiD"
                     /locus_tag="PK134_02225"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRB37702.1"
                     /translation="MYRREFLSSIAGIGAIGTLSSFKNITDDLSNSDKMPVLFIGHGS
                     PMNGIENNEFSNKWKKLGQSLPKPKAIVVVSAHWLTKGTWITAMNHPKTIHDFGGFPQ
                     ALYNVQYKAPGSPELAQETKNIIKSVNADLDHDWGLDHGAWTVTRRMYPDANIPVLQI
                     SIDINKPASFHYQLGKELASLRTKGVLIIGSGNMVHNLGKIAWDKIDEVGFGYDWAVE
                     MHQLFKSKISDNDHQALINYASLSKSALMAVPTPDHYFPLMYVLGLQQKNENAEFFND
                     KLVAGSINMTSVKFG"
     gene            complement(6898..7743)
                     /gene="accD"
                     /locus_tag="PK134_02230"
     CDS             complement(6898..7743)
                     /gene="accD"
                     /locus_tag="PK134_02230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRB37703.1"
                     /translation="MTWFKRIKEGITTSTREKKETPEGLWYKCPSCKAIVPSLEHEEN
                     KYVCPKCNYHDRIGSEEYFAVLFDDNQFTEFNTELTSADPLGFVDTKKYTDRLRDTIS
                     KTKLKDAMRTAVGKVNGNDLVVACMDFQFIGGSMGSVMGEKIAGAIDYSIQHKMPLMI
                     INKSGGARMMEAAFSLMQMAKTSAKLTRLADAGLPYISLLTDPTTGGVTASFAMLGDL
                     NIGEPGALIGFAGPRVVKETIGKDLPKGFQTSEFVLEHGFLDKIVDRRQLKDIIGQIL
                     SMFKN"
     gene            complement(7804..8859)
                     /locus_tag="PK134_02235"
     CDS             complement(7804..8859)
                     /locus_tag="PK134_02235"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRB37704.1"
                     /translation="MLNKITELLGKDADKLLNHVCKTVPKETLHQPGNDFVDRIFIPS
                     NRSNQVIRSIQSLYNHGRLNGTGYMSILPVDQGIEHSAGASFAPNPMFFDPENIVKLA
                     IEGGCNAVASTYGVLASVSRKYAHKIPFIVKINHNEFLTYPNKFDQIMFGTVDEAWNM
                     GAVAVGATIYFGSEESSRQIVEVAQAFERAHELGMATILWCYLRNPGFKKDGIDYHTA
                     ADLTAQANHLGVTIQADIIKQKLPTNNGGYTAINFGKTHKDVYAKLSSEHPIDLCRYQ
                     VLNCYNGRMGLINSGGESKGATDMAEAVTTAVINKRAGGQGLISGRKAFQKPFKDGVE
                     MLNAIQDVYLSKDVTLA"
     gene            8971..9966
                     /locus_tag="PK134_02240"
     CDS             8971..9966
                     /locus_tag="PK134_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB37705.1"
                     /translation="MKIVIPVAGIGTKLRPHTHTQPKTLVPVAGHPILAHIIDYFVQS
                     GYDEFVFIIGYMGEKIEEFVKSKYPSLKAAFVLQEPREGTGHSVWFARNHVHAEDEML
                     IVLGDTIIDFDINVFKQAEQSLLGVKKVDDPRNFGVVELNDEGFVSKLHEKPVIPKSN
                     LALVGIYFIKQAGHLFEALNYIIENNLKTRDEYHLTDAMMRMIEEGEKIKVFHVGNWF
                     DCGNKESLLETNAILLDRIAETNPKPQKFPNTIIIPPVSIAYNCDIADSIIGPHVSIG
                     EHTIVKYSIIKDSIIGSYSQLESAVLHRSVIGSDAFLKGLSQSLNIGDSTEIDFS"
     gene            10085..11041
                     /locus_tag="PK134_02245"
     CDS             10085..11041
                     /locus_tag="PK134_02245"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37706.1"
                     /translation="MKKLFTLITAVALGSLTSNAQIYDNGTIVNNPVLGVGGADVSSL
                     HDGLNSLGMNHASGLGYRVADDFIIPAGQTWTIDSIAFLAYQTNSGNASTITDVNCAI
                     FNGSPAIGGSIVLGDTITNLMQSTYFSNIYRTADAAFTNTARPVMRNIVVPQSAWSLP
                     AGTYWVAWQTGGTLTSGPWAPPLTNANMATGDAIQFNPDGSIWNPILDGGATADPQGL
                     PFQIYGTVTTSINSIEVEDALTILPNPSKGNFMINATFSNRCNLEINVMDLQGRSIYS
                     ENVSDVSVLTKNVVLNDVSKGIYLLKLTSDSGKVLTKKIVVD"
BASE COUNT         3537 a         1999 c         2037 g         3673 t
ORIGIN      
        1 tgatgaagat cttgcttctg ttactacagg tgccagaccg gctttgatat taggataact
       61 ttcagtaaat actgaaatgg ctcctgccgt tgaaaaccga tgaaacctgt cgttaagata
      121 aatgtatggc gttccattct ctacataacc ccataagcta tctcggttga ctttcaattt
      181 atgttcatct tttgcagtat aaaataagtt ttgtgtggtt gcccactggc cgatactgaa
      241 atcagattgg tcgtaatatg attttatgag atgatcttta gtaacagttg gtgtgttatg
      301 ttgcaggtct gttaatgtaa aataaacacc atcattaaat ttattaacct gtacggcatc
      361 ttctttcatc aacgcagaac cttgagaaaa agcttgaaca gaataaaaaa ttaataccgt
      421 gataagtccg gaaaatgttt tcattttatg aaatagtatt ctgcaaagaa acaaaaatca
      481 actcttataa gttatgctga tggctgttta aattgaacat catgaaggtg agcataaata
      541 cctttccttt ctaatagatt ttgatgattt ccgtcttcgg caattgctcc cttatgtaaa
      601 acaataatgc gatcagcttt ttctatagtt gataaacgat gggcaataat aattgaagtc
      661 cggttttttg tcagcatttc tgttgcatta accaaaagct gctccagctt actgtcaaca
      721 ctgcttgttg cctcatccaa aataattagt ttaggttgat gtgccaacac tctggcaaat
      781 gacaatagct gcctctgtcc ggtgctgatt aaccctcccc tttctccggg attgtaatgt
      841 agctgaccgg gtaacttttc tacaaaatgc caggctcctg tttgtttcaa caaggcttca
      901 ataacaatat catcttgttc tctatagagt gtaatattgt tctttatact gtcagaaaaa
      961 agaaagacat cctgaaggac aacgcctata gcatttctca ggctgtataa atcataatct
     1021 ctgatatcaa caccgtcaat taaaatctgt cctttttgaa tttcatataa gcggttaatc
     1081 aaacttacaa tagtagtttt ccctgcgcct gttgatccaa caatcgctac agattgtcct
     1141 gaaggaatga taaaggataa gccacgcaaa atccactcct catttttata ggcaaaccac
     1201 acatctctga attcaatttt gcctattata ttatcggcac tttttgtgcc tgaattaact
     1261 tctgtaaatg gttcatcgag caacttgaaa actctttctg aacttaccat gcccatttgt
     1321 aaggtgttaa acttatcagc aagttcacga attggcctga acaattggtt gatatattga
     1381 ataaaagcaa caagtaagcc taaactgata gaactttgaa gtacatttaa accaccatac
     1441 caaactataa gccctattga aacagcagag agaatttcta caactgggaa aaacaaactg
     1501 taataccata tagaacggat attggcatcg cgatgcttgg cattaatttc atgaaactta
     1561 tttaactcct cttcttcacg attaaaaagt tgtacaattc tcattgcaac taaatgctcc
     1621 tgaacaaatt cgtttagttt agctacctgt atgcgcacat cattaaaagt ttttctgata
     1681 tttttctgaa aaactctggt agcaatcaac agaacaggaa tagtagataa agaaattgca
     1741 gcaagttgtg gatggatgta aaacattaca gcaataatta ccactacctg aagcatatca
     1801 cttattataa gaatcaaccc atcagaaaat atatctgcaa tggtttccat atcgcttacc
     1861 aatcgtgtaa ttgtaattcc aagaggagtt ttatcaaaat aagctagccc aaaaccgctg
     1921 accttcttaa acaaattatt acgtaatgaa agtattactt cctgtccaag tcggtttgtt
     1981 aaggtagtat agacaaactg aagcaatgat tgtagtagca gaacaccaaa catgaaagca
     2041 acagtgcgtg taagaccttt gatgtcgtgt tttgcaatgt caatatcaag tgcctgctgt
     2101 gtaagccagg gtcgtaatgg tgccaataca gcaagcaaca cagtgataac cactgtggct
     2161 acaaaaagaa accgaaaagg ctttacatag aacataatcc tgtttagcac cttccaatta
     2221 aatgctgttc cgcttgttga cattattttt gaatagactg caaaattcac actcttattg
     2281 attagttcaa aatgaaagga gttttttgga atgtaaagtt cagcggtaat tgctattttt
     2341 gcacgctaat gacaagggaa tctactgtag cttttcacac cttaggttgt aaattgaact
     2401 ttgctgagac ttcaacaatt ggccgtcaat tgcaagctgc aggctacaaa aaagtttcgt
     2461 tttctgatac tgctgatgtt tatgtcataa atacctgttc tgttactgaa aatgctgata
     2521 gagaatgccg ccaacacgtc aacagagctt tagcagcaaa tcccgaagga tgtgtcatca
     2581 ttattggctg ttatgcacag ctaaaaccag atgcaattgc tacaattccg ggtgttagcc
     2641 ttgtattagg tgcaagtgaa aagttcaaca ttccgcatta tttgcaaaag cttaaaaagc
     2701 aaaccgaagc acaggttttt tcttgcgaca ttgccgatca tcatcagttt acaagcagct
     2761 actccgaagg tgacagaact cgtgtttttc tgaaagttca ggatggctgt gattatggtt
     2821 gcacattttg caccatacca ttggcgcgtg gcgcaagcag aagtaataca attgaaaatg
     2881 tcatagaaaa tgtaaaacaa attgttgata gaggtgcaaa agagattgtt ctgacaggag
     2941 ttaatttagg tgactttggt aaactgcctg gtgagaaaaa gcatgcaaca gaattttttc
     3001 aattagtttc tgcattagat gctttggatg ctgatatccg attcagaatt tcgtctattg
     3061 aaccaaacct gctaacagaa gaaatcattg aattggttag ctgctctaag aaatttatgc
     3121 cacattttca tattccatta caaagcggtt ctgataaaat tctgaaactg atgcgcagac
     3181 gttacctgac ttcgcattac acatcacgga taaataaaat taaaaagttg atgcccgatg
     3241 cctgtattgg tgttgatgtg attacaggat ttcccggtga aacggaggaa gattttcttc
     3301 agacttataa atatttgaac gaagctgata tttcttacct gcatgttttt acttattctg
     3361 aaagagcaaa cactcctgct attgaaatga aacctgttgt tccggttgca gaaaggaaaa
     3421 gaagaaccaa tatgctgcgc atactttcgg agaaaaaact cggaatattt tatcggtcac
     3481 aagaaggtaa aacagccaaa gtactttttg aacacgaaaa caagaatgga ataatgtcag
     3541 gctattccga aaactatgtg agagtttcaa cacctttcaa taacgaaatg gtaaataaag
     3601 taattgaatg taaaatcggt gcaaccgaca atgacagcca tgaagttaac tgtacaattt
     3661 tagaatcaat cacggcttaa taatttctgc ccatgtaccc tactctatcc gatgcactcc
     3721 atgatttatt gggcataaat attccattgc caattcaaac attcgggctc atattggcat
     3781 tgtcattttt tttggctgca tatattttaa tcttagagtt taaacgcaaa gagcaaaatg
     3841 gtttattaaa gtctttcacc gttactacca ttaaaggaaa acctgcatca acagcagagt
     3901 tgttttggtc aggtgttgta ggatttttag ttggctttaa attgcttgat gccattttga
     3961 attacagtga ccttgttaat gatccacaag gctttttatt atcaacaaga ggtaatgtca
     4021 ttggcgggat attactcggt gcctattctg tttggagcaa atacaaagaa aaagaaaagc
     4081 aaaaattggc agtgccgcag actgttacag aagttgggta tcctcatcag tttgtaatga
     4141 atattacgat tgctgctgcc gttgcaggat taattggtgc caaaatattt cataatcttg
     4201 aaaacattga tgactttctt aaagatcctg ttgatgcatt gatatcgttc agcggtttaa
     4261 ctttttttgg cggattgatt tgtggtgctt tagctgtgat atggtatagc aataaaaaat
     4321 taaatgtgcc gccattggtg atgtgtgatg ttgctgcacc tgcattaatg ctgacttatg
     4381 gcacaggcag attaggttgt cacttttcag gcgatggtga ctggggaatt gtaaatacac
     4441 ttgcaaaacc ttcttggttt ccatttcctg attggatgtg gagttataac tatccacaca
     4501 atgtaattgg agagggtgtt cctattgctg gatgtgttgg caaacattgc tatgaattga
     4561 tacctcctgt ttttcctaca ccattatatg aagcaattgc ctgtatttta ttattctttg
     4621 ttttgtggaa gctacgaact agaatagtta ctcccggaat acttttcttt gtttacctgc
     4681 tattcacagg tgtagaacgt ttggcaattg aacagatcag agtcaataca aagtaccaca
     4741 tatttggaaa agcaattaca caggcagagc ttatagctgt tgcatgtatt attgtttcaa
     4801 tcgttggaat tacgctttta agaagaaaaa attccggttc atcttaaagc ctgttgactt
     4861 taaaagaata ttgtggtaat ttggacaaaa ttttttgacc aaaaccatat atgctccata
     4921 aaagtttttt tacggcaata gtttttttgt ttctctccac atcgcttttt tcacaaaaga
     4981 ggattaaaat tcctgtactt gattatggtt ctgttgatga gtttgtgtta aagttaaaac
     5041 ccgaacaatg cgttaacatt gatacattag ctgaagtaat cagcagagaa tttgaccgcc
     5101 ctcattttcg tttcagggct gcattcagat ggattacaga aaacatagct tatgatttaa
     5161 agaaccaacg ccatccgcag aaagcgaatc tcaacgctaa cgatgttttt aagaacaaaa
     5221 ctgcactagc cgaaggctat gcaaatattc tgaatgctat ctgtgctaaa atgaatatca
     5281 catgcctttc ggtgaatggc tatttaagaa gaaatcaatt accgggaaca aagattaaaa
     5341 aaccaaatca tatctggagt actgttaaac tatatgataa atggtacttg atggatgtga
     5401 tgatggcaag tggctatttt gaaaaaggtg ataagatttt tacaaaacgc tttactgaga
     5461 gatattttgc attacatccg cgtcagataa ttttaacgca tcttccatta aatggaaatc
     5521 gtcaatattt agatactatt gttgacaaga atactttttt gaactatcct gaagtttatg
     5581 atttcttttt tgataacggc atttatgcat taagacctgc tgatggtgta ttacctgttt
     5641 acaaaggttt gaagaaacgt ttttatttta aatctatgga tagccttgct attggtaaga
     5701 ttgaaattcg tgatggtaaa aaatctgaaa agattcaaaa tacttttaca caggttgata
     5761 gtcttgttta tttcgattat gtatttgaat cgaataaaaa aagaacattg gacgtgtttg
     5821 ttgatggact accattatgt acttatttta tagttcccga taaaaagaaa aaacggtcag
     5881 aatattaact gttggaatct gcgtttaccc gaattttaca gaagtcatat tgattgaacc
     5941 tgctaccaac ttatcgttaa aaaattctgc attttcattt ttttgttgca gcccaagtac
     6001 atacattaac ggaaaataat gatccggtgt tggcacagcc attaaagctg acttgctaag
     6061 agatgcatag ttaatcagtg cctgatgatc gttgtctgaa attttagatt taaaaagttg
     6121 atgcatttca acagcccaat cataaccaaa gccaacttca tcaattttat cccatgcaat
     6181 ttttccgagg ttgtgaacca tattaccact tccaattatc aatactcctt ttgtgcgaag
     6241 tgaagcaagt tcttttccca attgataatg aaatgatgcc ggtttattaa tgtcaatact
     6301 gatttgaagc acaggaatat tcgcatcagg atacattcgt cttgtaacag tccatgctcc
     6361 atggtcgagc ccccagtcat gatctaaatc agcatttact gacttgataa tattttttgt
     6421 ttcttgtgcc aattcaggac tgcccggtgc tttgtattgt acattataaa gtgcttgcgg
     6481 aaatccacca aaatcatgaa tagttttagg gtggttcatt gcagtaatcc aagttccttt
     6541 tgtcagccaa tgtgcagaaa caacaacaat tgcttttggt ttaggtaatg attgacctaa
     6601 ttttttccat ttattgctga actcattgtt ttcaatgcca ttcattggag agccatgtcc
     6661 gataaacaat acaggcatct tatcggaatt actcagatca tcagtaatgt ttttaaaaga
     6721 acttaaagtt ccaatagctc ctatacctgc aattgatgat aaaaattctc tgcgatacat
     6781 ttgattttta ataattatca ataacgttta cagcataaac attgtttact tgtaacaaac
     6841 taaaacatgt aaaggattag ttgaattatt tattcagaaa aagtaaactg aaagcaatta
     6901 gtttttaaac atgcttagta tctgaccaat gatatctttt aattgtctgc ggtcaacaat
     6961 tttatctaaa aaaccatgtt caagaacaaa ttctgaagtc tgaaatcctt tgggcaaatc
     7021 tttacctatt gtttctttta caactcgtgg accggcaaat ccaattaatg ctccgggctc
     7081 tccaatgttt aaatcaccaa gcatggcaaa agatgctgtc acacctcctg tggtaggatc
     7141 ggtaagtaaa ctaatataag gcaagcctgc atcggcaagt ctggtaagct ttgctgatgt
     7201 ttttgccatc tgcatcaaag aaaatgctgc ttccatcata cgggcaccac ctgatttgtt
     7261 tataatcatc aatggcatct tatgctgaat actatagtca attgctcctg caattttttc
     7321 gcccattaca gagcccatac ttcctccaat aaactgaaag tccatgcaag caacaactaa
     7381 atcgttaccg ttaactttac ctacagcagt acgcatagca tctttcaact tcgttttaga
     7441 aatggtatca cgtaagcggt cagtatattt ttttgtatca acaaaaccta aaggatcggc
     7501 agaggttagt tctgtattaa actctgtaaa ctgattgtca tcaaaaagaa ctgcaaaata
     7561 ttcttcagaa ccaattctgt cgtgataatt gcactttgga catacatatt tattttcctc
     7621 atgctccaat gatggaacaa tagctttaca tgatgggcat ttataccaca aaccttcggg
     7681 agtttctttt ttctctcttg tagaggtggt gatgccctct ttaattcgtt taaaccaagt
     7741 catttataat cagtttgcgt ttttaaatgt tgggtttatc aactgatata ctgtttatat
     7801 tacttatgct aaagtaacat cttttgaaag atatacatcc tgtattgcat ttaacatttc
     7861 aacaccatct ttaaatggtt tttggaaagc tttacgtcct gaaattaatc cctgaccacc
     7921 ggctctttta ttgataactg ctgtggttac tgcttcggcc atatctgtag cacctttaga
     7981 ttctccacct gaattgatta atcccatacg gccgttataa caattcaata cctgatagcg
     8041 acaaaggtca ataggatgtt cagaagaaag ctttgcataa acatccttat gagtttttcc
     8101 gaagttaata gcagtgtatc caccattatt agtaggtagt ttttgtttaa taatatctgc
     8161 ctgaatagtt actcctaaat gatttgcctg agcagttaag tctgcagcag tatggtaatc
     8221 tattccgtct tttttaaatc ccgggttgcg tagataacac cagagaattg ttgccatacc
     8281 cagttcgtga gctcgttcaa atgcttgtgc cacttctaca atttgtcttg atgattcttc
     8341 gctgccaaaa taaattgttg caccaacagc cacagcgccc atgttccaag cctcatcaac
     8401 agtaccgaac ataatctgat caaatttatt tgggtatgtc agaaactcat tatggttaat
     8461 tttaacaata aaaggaatct tatgagcata ctttctggaa acagatgcaa gtactccata
     8521 agttgaagca actgcattac agccgccttc tatggctaat tttacaatgt tttcagggtc
     8581 aaaaaacatt gggtttgggg caaagcttgc tccggcagag tgctctattc cctgatctac
     8641 cggcagaatg gacatatatc ctgtaccatt caaacgacca tgattataga gtgattgtat
     8701 gctacggatt acctgattac ttctgttcga agggataaaa atgcggtcaa caaagtcgtt
     8761 acccggctga tgtaatgttt cctttggaac agttttacaa acgtgattta ataatttatc
     8821 ggcatccttg ccaagtaatt cggtaatttt gttcaacata tcgtgcgttt aaatatcagt
     8881 taagtgtggc aaaactacat ttttttttat tccatgccat cagacttatg ctgttctgta
     8941 gtttaatatt tttgcttaaa ataatttgaa atgaaaattg taatccctgt ggccggtatc
     9001 ggaacaaaac ttcgtccaca tacacataca caaccgaaga cattagtgcc tgttgcaggc
     9061 catcccattc ttgcccacat cattgattat tttgtgcaat ccggatatga tgagtttgta
     9121 tttattattg gttacatggg tgaaaaaatt gaagaatttg taaaaagcaa atatccttca
     9181 cttaaagcag catttgtatt gcaggaacca cgcgaaggta ccggtcattc tgtttggttt
     9241 gctcgaaacc atgttcatgc agaagatgaa atgctcattg tattgggcga tacgataatt
     9301 gattttgaca ttaatgtttt taagcaagcc gaacagtctc ttttaggagt taaaaaagta
     9361 gatgatccaa gaaattttgg tgttgttgaa ttgaatgatg agggtttcgt ttccaagttg
     9421 catgaaaaac cggtaatccc aaaatcaaat cttgcacttg tcggtatcta ttttattaaa
     9481 caagcagggc atttgtttga agcattgaat tacattatcg aaaacaacct gaaaacaaga
     9541 gatgaatatc atctgactga tgccatgatg cgaatgattg aagagggtga gaaaattaaa
     9601 gtctttcatg tgggcaattg gtttgattgt ggaaacaagg aaagtctcct tgaaaccaat
     9661 gcaattttat tagacagaat tgcagaaaca aatcctaaac cacagaagtt tccaaataca
     9721 atcataattc ctccggtgag tattgcatac aactgcgata ttgctgattc cattattggt
     9781 cctcatgttt ctattggcga gcataccatc gtaaaatatt ctatcattaa agattcaatt
     9841 attggctcct attcgcagtt agaatctgca gttttacatc gttctgtaat tggaagcgat
     9901 gcatttctaa aaggactaag tcagagcttg aatattggtg atagtacgga gatagatttt
     9961 agttgatgtt ataaacatat tcattggaag taatgttgat attttaagaa atctaatgta
    10021 aaatatagtg attgtaatat ttaaaccttt acttttgata gataatttca aaatcaaaaa
    10081 aaatatgaaa aaactattta ctcttattac agcagttgcc ttaggaagtt taacttctaa
    10141 tgcacaaatc tatgacaatg gtacaattgt aaataatccg gtattaggtg tcggtggagc
    10201 agatgtatcc tccttacatg atggactgaa ttcattaggt atgaatcatg cttccggact
    10261 gggttatcgt gttgctgatg atttcataat tcctgcaggt cagacatgga caattgattc
    10321 tattgcattt cttgcttatc aaaccaattc tggaaacgca tctacaatta cagatgtaaa
    10381 ctgtgcaatt tttaatggtt caccggcaat tggtggttct attgttttgg gcgatacaat
    10441 aactaatttg atgcaaagca cttatttctc aaatatctat cgtacagcgg atgcagcatt
    10501 tacaaataca gcgcgtccgg taatgcgaaa catagttgtg ccacaaagtg catggtcact
    10561 accggctgga acttattggg ttgcctggca aacaggcggt acattaacat ctggtccctg
    10621 ggcacctcct ttgactaatg ctaatatggc aaccggagat gcaattcagt tcaatcctga
    10681 tggttcaatt tggaatccaa ttctggatgg tggcgctaca gctgacccac aaggcttacc
    10741 atttcagata tatggaactg taacaacttc catcaattca atagaagtag aagacgcact
    10801 tacaattcta ccaaatccat caaagggaaa ttttatgatt aatgctactt tttcaaaccg
    10861 ttgtaatctg gaaattaatg taatggattt gcaaggcaga agcatctact cagaaaatgt
    10921 tagtgatgtt tctgttttaa ctaaaaatgt tgttttgaat gacgtttcca aagggattta
    10981 tctgttgaaa cttacaagtg attcaggtaa agttcttacc aagaaaatcg tagttgacta
    11041 ataattttat aaacttttta aaggctgtaa tttttagaaa ttgcagcctt tttttttaaa
    11101 aaattgtaga acaaactcga ttagcctatg aatatttcta tagttgaatt gtattatatg
    11161 ggtattgaat taagtttgta aaaaatttag atatgaaaaa attatttact ttattatttg
    11221 taatacttat ttgctatatt cagggt
//
LOCUS       DAORQT010000031        10917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_729_length_10917_cov_15.601731, whole genome shotgun sequence.
ACCESSION   DAORQT010000031 DAORQT010000000
VERSION     DAORQT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_729_length_10917_cov_15.601731"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK134_02250"
     CDS             complement(<1..295)
                     /locus_tag="PK134_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37707.1"
                     /translation="MKLKFIFVFLASVLLVTDESKAQNRNSIWCFGDSAGINFSNINN
                     PVPFTSSVEGRGSCVSIADNNGNLLFYSNTWMYTARTIVWNALNDTMTNSYPMG"
     gene            complement(349..7866)
                     /locus_tag="PK134_02255"
     CDS             complement(349..7866)
                     /locus_tag="PK134_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37708.1"
                     /translation="MKRIYSLLIYIVFINTFPALATTYTWTGNTSTAWNTTSNWSPSG
                     TPTTGDFVHIYSATYNPQLTGNLTLNGIYIHSGTLNLNGDTLLLNSGTSTFSGGTISN
                     GLLQLRGTEITFNSTTFNAPIDAVVDRINLNGCTFNNYCYFETISNVPGTGSGGAVFN
                     DSVYLYHNYTGSGIWVMNNNTGHRFNGKVTIHNKGTKEITFTGAGINVFNGDIVLINT
                     GAGGIMLGNDTLTAGHTISIGSGGFSAGDFNATNFYQDGNTAISLTFTGTALPSITGA
                     TFGGNFSITSNVLLFKNCTFSGTASFTATGSGSSSWYGGNTFYGNATFTNNATAGNLR
                     IDADTANTYYADATFNGSRELRVCYGGEVSSFYGNITTANNTTFNAGTGTLCLAGSGT
                     QALSFHSNTLVKNLMLNKSGGSAALSTALVVSGTVTFTKGTLTTTSSNLLTINNGGTV
                     SGASDSSFIDGPVKKIGNSAFVFPTGKGNSYRAVEISAPSNTTDAYKAEFFYDSISVN
                     TSNIDTTLGYVTRNRYWKLNRTNGSSNVYVSLYWGGSYAVHDTAKTYISGWNGTKWLN
                     LGKVIYGNNNSGKVTSSGTVTSYSEFALAISSTNPMLCASTPQIIFPKEYFTFDVVSG
                     SGYDYNQSSDPLTPSIASTSSSFALPSITSANIGTLSGTTQNYLKNESSAASSLTCSN
                     PVDLTKGVICVEMLVKLDANFVNQRGLRFFNFKDNSGDNAFYGGIGYPYLYLNAKVID
                     NISDPNNPIQIEKRLQIPLDGVDRKSIDYYNDQGWHHFVFKINTHNNENPPSTAPGRL
                     EIWVDGELNDFYLDDADLNNSLSILQPEFRLLDAGIDCFLGKMDNIAIYDDDVCPEQL
                     YNNYKTCFPLLTNLEPVANYCYDNTNCDLSLITSIPTTPLTGAMNPNDFPENYTINTA
                     PLSDGDLIDYINSTCSCNSSDPNWFVELDDFLQNSYDAQFTGVALPLDQLKSYPLPRY
                     FPGHSFRQNVNFMGVNYLGYSGYPRSGGSYSISANAPTYSTDIQKELSENWNYSFVVC
                     ENSSTYPNTFTQAWINAANSYPADSPVKFAITTNRNHLPNSKVKKQDFVPDEDYYLMS
                     YDWNPTPPTLSPNQFLDSDGNIVSSPKILKPLNDPTYLSNFLPDGFDQNVIFSSIFGS
                     LTKNVSYVIENNEYTPQFSYNSSGIPAVANTINGDYTLSQGCSTCATPDDWKSYLGEF
                     KKTLDNDYRDQFFGGTNFPQLADAVYLNYAVDGFDKYNKNYRFEYTNMRETQSQVMYG
                     GSSQYLSTPDFYPRRAANWLTTVSADHGIDWVNKCRTNEIDNFSDYRYAPFVAAGWEQ
                     QEEFNIRPAQWLSLVKAAGIMGVDFYNTGYFNLNAFWFANPSGYVWQAVIPSYAQAVF
                     SHAEDYFTNGTLSKGNVLLEVTDASFGYGYRFAGSLPNDLIMIRKINNEDKILITGSL
                     QPLSNISGNVPDDRDIVINDLPFSSTTSAKINFRRQGSTYIFDNTNPGDPVFYQLDKW
                     HEYKHPERWSKDFDFEAEVFDNKNVNVVIKTVDATSNSSITNGDFTNYTSYITFAATP
                     DNTGVQYNFTPRADGVNNDYDIWVRARSRSGTRTGFTITTTSVGNSNFTQDNKVSCVS
                     SGSWEWYKLDDCTGSAMTLSSLTDDQQYVLTLLPMSDDLEIDRILLDVDNNAGFTAAS
                     ACGYDKDIPDNTSSTTVSISNNDFVNIQGTYIVNSNLSLTGCIINVEAGGEIDVQSGG
                     TFSCDNCHISACGDMWNGINNTGGTVTLTSSTVNDALAAVKSVSGNAVDVTGCVFDHN
                     YTAIWLENGSYSASDIYGNTFENTGGQINKAPHAGDDPLHHIYLNNVSAVTVGDASQA
                     VNTFHNALNGIYAEASNLNVYNNIFNNIGGNNYQNRAIVPPVPPISIPNAAINARGVK
                     AGYKTLNVGGASTGQSNTFNQCEIGVSGYMQLNADIRNNAFAECYTGIALSSIGGSGN
                     TVNIEDNTIDKYNKGISLSNVWMNNSTVIKDNDLNLGAGYDAAHYGAMGIEVSNFITR
                     QVKLTIENNHIENAQNGIQVSNVGYTISPVGYAPYADVKDNTVLFAIPVGLTANHVGL
                     WLENMAYGTVENNTVHWTETPSGAGSELMQGLRLSNSVHCGITGNAIENMESGIMVFG
                     NCSQTNLYCNKFESCFNSTYFSNNNSSNQLTDQGLKVETMGTYDPTLSEGWKNHWVSP
                     VSDKAAGVLLAPNIFNWMYKPSVSFDYDPGNFTGYFTTLSADNETGSCTPAFTTDEGL
                     RDQAFGSLVYDSVIAHTDSSEIKYLMEEVFYKLMRSDTSWLHLHVPSDSVYIDRYNAG
                     KSGNAGNFATVQDSIASQNYSSASGILTGITSSLEQDLDKKFVLDCYLKYIADTLTLD
                     SATFENLNKVAVQHPLYGGEGVYFARAMLNLDIIDILPGEEESRMANTASPPENKFID
                     QQDGKLYPSPTKGRVIFEMSSAFGASAKLDIYNSYGSLAASYPLNPNARSAEFNLSGL
                     KPGVYSCRLYDSINLKMINKLVIIR"
     gene            complement(7921..8331)
                     /locus_tag="PK134_02260"
     CDS             complement(7921..8331)
                     /locus_tag="PK134_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37709.1"
                     /translation="MQPTKLKSGTVCGNLLAALLLFSTSSIAQPSDAYVSMKATAVAG
                     TVHGVYYIELADTNSVNQIEIKLGTDWTSADMVNAVFTYDTPGSLPQGYSYTRNGNII
                     AIDMGNFPEQSSYYGNVRVKNGATWSNNYTFVSN"
     gene            8484..9686
                     /locus_tag="PK134_02265"
     CDS             8484..9686
                     /locus_tag="PK134_02265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB37710.1"
                     /translation="MKILQLCLRIPLPPYDGATIAMYNLAESLTASGAEVTMLSFNTK
                     KHFTDINSIDAALLEKYKLETVFLDASVNAWDAFANLFKPNESYNIVRFDSEAFHQKL
                     KELLQSETFDFILFEGLFLSPYLQTVRTFSKAKCILRAHNVEWLIWKRLAKATKNPLK
                     KTYLYFLSDRLKHYENIAINKFDAIIAISQPDKNLFLKDGCNIPIEVAPTGMNTAKYK
                     NIEQMDADSLSLFHLGSMDWLPNIEAVDWLLKDIWPLINQQSKKIKLHLAGKNMSPKY
                     FNLKSENLIIEGEIKDSKKFMQDKQIMVVPLLSGGGIRIKILEGLAAGKVIVTTSIGA
                     EGINYTDKKNILIADTPQQFADTILALLNNPEKLNSIAKEAQLLARSQYNNNIIGEQL
                     VRFYQTIR"
     gene            complement(9704..10390)
                     /locus_tag="PK134_02270"
     CDS             complement(9704..10390)
                     /locus_tag="PK134_02270"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB37711.1"
                     /translation="MTSPEVFQFGIPVTSVIDSINITGQEIAFYRITNIDALYSKLSS
                     LKNDDDNVIDERIPYWAELWPSAIALAEYMCTQKLNNDILIHEIGCGLALPSLVCGKM
                     GYSVMMSDYVDAPLQFASANWRLNVKSEPTTILLDWRKIPDDIEKCDLLLAADVAYES
                     RMFPAIVNAFKRLVKSGGRIVFTEPNRKFASGFFELIRHEFTNTTIQNREVIQNNHKH
                     QIKIVEIEVE"
     gene            10437..>10917
                     /locus_tag="PK134_02275"
     CDS             10437..>10917
                     /locus_tag="PK134_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRB37712.1"
                     /translation="MQIVFSFILNEINSNYQLYVVQANYPSEKESRKTFQKELNTKLL
                     KQVTKNPSLQLHYQETGKPYIDGNNAISFSHSGNYTALMITQSKSAGVDIEMTDRNIE
                     AGIDYFLNTNEIAFLSLKEKNLQALTCWCIKEAAIKYFNVSGIDFKNCIHIQPFELLN
                     "
BASE COUNT         3290 a         2178 c         2103 g         3346 t
ORIGIN      
        1 cccccatggg gtatgagtta gtcatggtat catttaaagc attccatact attgttcggg
       61 ctgtatacat ccatgtatta ctataaaata aaagattacc gttgttgtct gctatgctaa
      121 cacaagaacc tctgccttca acagatgaag taaaaggcac agggttattt atgttactaa
      181 aattaatccc tgcgctgtcg ccaaagcacc agatgctgtt tcggttctgc gcttttgatt
      241 catcggttac taataacaca gaagctaaaa acacaaaaat gaacttgagt ttcatatagc
      301 aaatataatc aaaaaggggc tgctacagca accccttttt gaaaaatact aacgaataat
      361 aactaattta ttaatcattt taagattaat gctgtcgtat aacctgcacg agtaaacacc
      421 cggctttaat ccggataaat taaattcggc agaacgggca ttgggattaa gcggataaga
      481 tgctgcaagg ctgccgtaac tgttgtaaat atccagttta gcacttgctc caaatgcgga
      541 actcatttca aaaataaccc tgcctttggt gggacttgga tacagtttcc catcctgctg
      601 atctatgaat ttattctctg gcggtgatgc ggtgttagcc atgcggcttt cttcttcgcc
      661 aggcagtatg tctattatat ctaaattaag cattgcccgt gcaaaataaa cgccctcacc
      721 accataaagc ggatgctgaa cagctacttt gttcaggttt tcaaacgtag cggaatcaag
      781 cgtaagcgtg tcggcaatgt attttaaata gcagtcaagc acaaactttt tgtcaagatc
      841 ctgttctaac gaagaggtga ttccggtaag gattccggaa gccgaagaat aattttgtga
      901 agctatggaa tcctgaaccg ttgcaaagtt tccggcattg ccggatttgc ctgcattgta
      961 tcggtcaata taaaccgaat cggaaggcac gtgcaggtga agccatgagg tatcgctccg
     1021 catcagttta taaaatacct cttccatgag gtattttatt tcggacgaat cggtatgggc
     1081 aataacagaa tcataaacaa gagaaccaaa tgcctggtcg cgcaagccct catcggtggt
     1141 aaatgctggc gtgcagcttc cggtttcatt atcggcagat aatgtggtaa aatagcctgt
     1201 gaaattaccc gggtcataat caaaagagac tgaaggttta tacatccagt taaatatatt
     1261 aggagctaat aaaactcctg ccgctttatc gctaacgggg cttacccagt ggtttttcca
     1321 gccttcgctc agggttgggt cataagttcc catagtttcc actttcagac cctgatcagt
     1381 tagctgattg gaagaattgt tgttactgaa atacgtggag ttaaaacatg attcaaactt
     1441 gttgcaatac aaattggttt ggctgcaatt accaaacacc ataatgcccg actccatgtt
     1501 ttcaatggca ttgcctgtaa tgccgcaatg cacgctgttg ctcaggcgca agccctgcat
     1561 aagctcactg cccgcaccgg agggtgtttc agtccagtgc acagtattgt tttcaaccgt
     1621 accgtaagcc atgttttcaa gccataagcc tacatggttt gccgttaatc cgacaggtat
     1681 ggcaaacagc accgtgttat cttttacatc agcataaggc gcataaccca ccgggcttat
     1741 ggtgtaaccc acattgctca cctgtatgcc gttttgcgca ttttctatgt ggttgttttc
     1801 tattgtgagc tttacctgcc gcgtaataaa attacttact tcaatgccca tagctccgta
     1861 atgcgcggca tcatagcctg cacccaggtt caaatcatta tcttttatta cagtggagtt
     1921 attcatccac acatttgaca aggaaatgcc tttgttgtac ttgtctattg tattgtcttc
     1981 tatatttacg gtgttgccgc ttccgccaat ggagcttaat gcaatgccgg tataacattc
     2041 ggcaaatgca ttgttcctga tgtcggcatt caactgcata taaccggaaa cacctatttc
     2101 gcattggtta aaagtattgc tctgcccggt tgatgcacct cccacgttta aggttttgta
     2161 accggcttta acacctcttg cattaatggc tgcattagga attgaaatgg ggggaacagg
     2221 aggaacaata gccctgtttt ggtaattatt accgccaatg ttgttaaaga tattattata
     2281 cacattcaga ttggaagcct cggcatatat gccgttaagg gcattatgaa aggtattaac
     2341 agcctgtgaa gcatcgccaa ccgtaacggc agatacgtta ttcaaataaa tatgatgcag
     2401 aggatcatcg cctgcatgag gcgctttatt aatttgcccg ccagtgtttt caaaagtgtt
     2461 tccgtaaata tcgcttgcgc tgtaactgcc gttttcaagc catatagccg tgtaattatg
     2521 gtcaaacacg cagcctgtaa catccacagc attgcccgaa acggatttaa cggcagcaag
     2581 ggcatcatta acggttgagc tggtaagcgt tacggtacct cctgtgttgt taatgccatt
     2641 ccacatatcg ccacaagctg aaatatggca gttgtcacaa ctgaatgtgc cgcctgactg
     2701 cacatcaatt tcgccacctg cttcaacgtt tatgatacat ccggtgagag aaaggttgct
     2761 attaacaatg tatgtgcctt gtatattcac aaaatcgtta tttgaaatgg aaactgtagt
     2821 gctgcttgtg ttatcaggaa tatccttatc ataaccacat gccgaggcgg cagtaaatcc
     2881 tgcattgttg tcaacatcca ataaaattct gtctatttcc aaatcatcgc tcatgggcag
     2941 cagcgtgagc acatattgct ggtcatcggt aaggctgctt aatgtcatag ccgagcctgt
     3001 acaatcatcc agcttatacc actcccatga accggaactt acgcacgata ctttattatc
     3061 ttgtgtgaaa ttgctgttgc ctactgatgt ggttgtaatt gtaaagcctg ttcttgtccc
     3121 tgatctgctg cgtgcccgca cccatatatc ataatcattg tttaccccgt cagcccttgg
     3181 tgtaaagtta tattgcacgc ctgtattgtc aggtgttgcg gcaaaggtta tgtaactggt
     3241 gtaattggta aagtcgccat tggtaataga agaattagaa gtcgcatcaa cggttttaat
     3301 aaccacattg acatttttat tatcaaacac ttccgcttca aaatcaaagt ccttgctcca
     3361 tcgttcagga tgtttatact catgccattt gtcaagttga taaaatacag gatcaccagg
     3421 attggtattg tcaaaaatgt aggtggagcc ttgccgcctg aaattaattt tggcggatgt
     3481 ggttgaagaa aacggcaaat cattaatcac aatgtctcta tcatcaggca catttcctga
     3541 aatattgcta agcggctgaa gcgatccggt aatcagtatt ttgtcctcat tatttatttt
     3601 acgaatcata ataagatcgt tcggaaggct tcctgcaaac cggtacccat atccaaatga
     3661 agcatcagta acttctaaaa gtacatttcc tttagataat gttccgttag taaaataatc
     3721 ttccgcatgg ctgaatacag cctgagcgta acttggtatt actgcctgcc aaacatatcc
     3781 tgacggattg gcaaaccaaa aagcattcag attaaaataa ccggtgttat aaaagtcaac
     3841 acccattata ccggctgctt taaccaatga cagccattgt gcaggtcgta tattaaattc
     3901 ttcctgctgc tcccatccgg cagcaacaaa aggcgcatag cgatagtcgc taaaattgtc
     3961 tatctcattt gtcctgcatt tgtttaccca gtcaattccg tggtcagcac taacggtagt
     4021 taaccagttg gcagctctgc gcggataaaa atcaggggta gataaatatt gagaagaacc
     4081 gccatacatt acctgcgatt gcgtttctct catgtttgtg tattcaaatc tgtagttttt
     4141 attatactta tcaaaaccat caacggcata attcaaataa acagcatcag ctaattgtgg
     4201 aaaattagtt cctccaaaaa actgatcacg gtaatcgttg tcaagagttt tcttaaattc
     4261 tccaagataa gacttccaat catcaggtgt tgcacaagta ctgcatccct gagataaggt
     4321 ataatctcca ttaatagtat tagccactgc cggaattcca ctggaattat aactgaattg
     4381 aggagtatat tcattatttt caataacgta acttacgttt tttgttaaac taccgaatat
     4441 gctggaaaat ataacatttt gatcaaatcc atcaggtaaa aaattactaa gataagttgg
     4501 atcatttagg ggtttaagta tcttaggaga agaaacaata tttccatcag aatctaagaa
     4561 ttgatttgga gacagagtag gaggagttgg attccaatca tagctcatta aataataatc
     4621 ttcatccggc acgaagtcct gcttttttac cttagagttg ggtaaatgat ttctattagt
     4681 agtaattgca aattttacag ggctgtctgc cgggtaacta tttgcagcat ttatccatgc
     4741 ttgtgtaaaa gtatttggat aagttgaaga attttcacaa accacaaagg agtaattcca
     4801 gttttcagaa agttcttttt gaatatctgt actgtaggta ggtgcgttag cagatattga
     4861 ataagaacct ccgcttcggg ggtatccact atatcctaaa taattaaccc ccataaaatt
     4921 aacattttgc ctgaatgaat gcccgggaaa ataccgtggc agaggatatg attttaattg
     4981 atctaacgga agagctactc ctgtaaattg cgcatcataa gagttttgta aaaaatcgtc
     5041 caattcaacg aaccaattgg gatcagatga attacaacta catgttgagt ttatgtaatc
     5101 aattaaatca ccatctgata atggagctgt gtttatagta taattttccg gaaaatcatt
     5161 cggattcatt gcacctgtga gaggtgtggt gggtatggaa gttattaaac taaggtcgca
     5221 atttgtatta tcataacaat agttagcaac aggctctaaa ttggttagaa gaggaaaaca
     5281 cgttttataa ttattataaa gctgttcagg gcaaacatca tcatcataaa tagcaatgtt
     5341 atccatttta cctaaaaaac agtctattcc tgcatccaaa agccggaact ctggttgtaa
     5401 aatactcaat gaattattta ggtcagcatc atccaaataa aaatcattta attcgccatc
     5461 aacccaaatt tctaatcttc ccggtgcagt tgaaggtgga ttttcattat tatgagtatt
     5521 tattttaaaa acgaaatggt gccatccttg gtcattataa taatctatgc ttttcctgtc
     5581 aacaccatct aatggaatct gtaatctctt ttcaatttga atgggattat taggatcgct
     5641 gatattatca ataactttag catttaggta aagatatgga tagccaatac ccccataaaa
     5701 agcattatcg cctgaattat ctttaaaatt aaaaaaacgt aaccctcttt gatttacaaa
     5761 atttgcatca agtttaacca gcatttcaac acatatcacc ccctttgtta aatcaacagg
     5821 gtttgagcaa gttaaagaag atgcagcaga ggattcattt tttaaatagt tttgggttgt
     5881 tccagaaagt gttccaatat tggcactggt aatactagga agagcgaaac tgctggatgt
     5941 tgatgcgatt gatggggtta atggatctga agattgatta taatcataac cgctaccact
     6001 tacaacatca aacgtaaagt attcttttgg aaaaataatt tgtggagttg aagcacaaag
     6061 cataggatta gtagaagaga tagccaatgc aaattctgaa tatgatgtaa cagtgcctga
     6121 tgatgtaact tttcccgaat tattatttcc ataaattact ttaccaagat tcagccattt
     6181 agttccattc caccctgaaa tatatgtttt cgcagtgtca tgcaccgcat aactacctcc
     6241 ccaataaaga ctgacataca catttgatga tccattggtt ctgttcagtt tccagtatcg
     6301 gtttcttgta acatatccca atgtagtatc tatgtttgat gtattaacac ttatggaatc
     6361 ataaaaaaat tctgctttat aagcatcagt cgtatttgaa ggtgcgctta tttccacagc
     6421 tctgtaactg tttccttttc ctgtcggaaa cacaaatgcg ctgttgccta ttttcttaac
     6481 aggtccgtca ataaagctgc tgtcgcttgc tccactcact gtgccaccgt tgtttatcgt
     6541 aagcaaattg ctgcttgtgg tggtgagcgt tccttttgta aacgttaccg ttccgcttac
     6601 taccaatgcc gttgacaatg ctgcactgcc accgctcttg ttcagcatca gattttttac
     6661 aagcgtatta ctgtgaaagc tcaacgcctg tgttccgctg ccggcaagac acagtgtgcc
     6721 tgtgcctgca ttaaaggtgg tgttgtttgc tgtggttatg tttccgtaaa aggagcttac
     6781 ctcaccacca tagcacaccc ttagctcgcg cgagccgtta aaggtggcat cggcatagta
     6841 ggtgttggca gtgtcggcat caatgcgaag gttgcctgcc gtagcgttgt tggtaaaagt
     6901 ggcattgcca taaaatgtat tgccgccata ccaactgctg ctgccgctac ctgtggcggt
     6961 aaatgatgcc gtgccgctaa acgtacagtt cttaaataac agcacattgg aagtaatgct
     7021 gaaattacct ccaaacgttg ctcctgttat gcttggcaat gctgttccgg taaaggtgag
     7081 gcttatagca gtgttgccat cctgataaaa attagtagca ttaaaatcgc ctgccgaaaa
     7141 acctccgctt ccaatgctga tggtatgccc tgctgtgagc gtatcattac ctaacataat
     7201 tcctcccgct ccggtgttta ttaaaactat atcaccatta aatacattaa tgcccgcacc
     7261 tgtaaaagtg atttcttttg ttcctttgtt atgtatggtt actttaccgt taaagcggtg
     7321 tccggtgttg ttgttcatca cccaaattcc cgaccctgtg taattatgat acaggtaaac
     7381 actgtcgtta aacactgctc caccgcttcc cgtaccgggt acattggaaa tggtttcaaa
     7441 ataacagtaa ttgttaaacg tgcatccgtt caggttaata cggtcaacta ctgcatcaat
     7501 gggagcatta aaagtggtgc tgttaaatgt aatttctgtt ccgcgcagtt gcaacaagcc
     7561 attgcttatg gtgccaccgc taaaagtgct tgtaccggag ttaagcaaca aggtatcgcc
     7621 attcaaatta agcgttccgc tgtgtatata aattccgttt aaggttaggt tgcctgtaag
     7681 ttgcggattg tatgtagcac tgtaaatgtg tacaaaatcg cctgttgtag gtgttcctga
     7741 tggcgaccag ttagacgtag tgttccatgc agtgcttgtg tttcctgtcc atgtataagt
     7801 ggttgccagt gcaggaaatg tatttataaa cacaatatag atgaggagtg aatatattct
     7861 tttcatggta tttgattttt aaatgttaaa cattaaacat aatttgattt tataatacaa
     7921 tcagtttgaa acaaaggtgt aattattgct ccatgttgca ccatttttta ctcttacgtt
     7981 tccgtagtaa gaagactgtt cgggaaaatt gcccatgtca atggcaatga tgtttccgtt
     8041 gcgagtgtaa ctgtaaccct gcggcaggct gccgggtgta tcataagtaa acactgcatt
     8101 taccatgtcg gcagatgtcc agtcggtgcc gagtttgatt tcaatttgat tcacactgtt
     8161 ggtgtcggcc agttcaatgt aatacactcc gtgtacagta cctgcaacag cagtagcctt
     8221 catgcttacg taagcatcag atggttgggc tatgctgctt gttgaaaaca acagtagcgc
     8281 agcaagcaag ttcccgcata ctgtgccgct ttttaatttg gttggttgca tgatagttgg
     8341 gattttagtt tggtagtaat gttattttta aagatttgta aaattaatga gttttccttt
     8401 tcatatccaa attaatttat catcaaggtt taagggtgtt aattttttta agtttgcaca
     8461 taagtttaga atccttcttt aatttgaaaa tccttcaact gtgtttgcgt attccgttgc
     8521 cgccttatga tggcgccacc attgccatgt acaaccttgc cgaatcactc acggcatcgg
     8581 gcgcagaggt aacaatgctt tcgttcaaca ccaaaaaaca ttttacggac ataaattcca
     8641 ttgatgccgc attgcttgaa aaatataaat tggaaacagt ttttcttgat gcctctgtaa
     8701 acgcatggga tgcttttgcc aatctgttca aaccaaacga gtcatataac attgtccgtt
     8761 ttgactcgga agcgtttcac caaaagctga aagaactgct gcaatcggaa acatttgatt
     8821 tcatattgtt tgaaggatta tttcttagcc cttacctaca aacagtgcgt acattcagca
     8881 aagccaagtg catattgcgg gcacataatg tggaatggct tatctggaaa agacttgcta
     8941 aagcaacaaa aaatccatta aaaaaaactt atctttattt tttatccgac agactcaaac
     9001 attatgaaaa tattgccata aataaatttg acgcaatcat tgctatatcg cagccagata
     9061 aaaatctttt tctgaaagat ggctgtaata ttcctattga agtggcgcca acaggaatga
     9121 atactgccaa atacaagaac atagaacaga tggatgctga tagtctttca ttatttcatc
     9181 ttggatcaat ggactggctc cctaatattg aagccgttga ctggttattg aaagatatct
     9241 ggccattgat aaaccaacaa tcaaaaaaga ttaagctaca cttagccgga aagaatatgt
     9301 cgccaaaata tttcaatctg aaatcagaaa acctgattat tgaaggtgaa atcaaagact
     9361 cgaaaaagtt tatgcaggat aaacagatta tggttgttcc gctattgagt ggtggtggca
     9421 ttaggattaa aatattagaa ggacttgcag caggaaaagt aattgtaaca acaagcatag
     9481 gtgcagaagg aattaattac acagacaaaa aaaatattct tattgctgat acgcctcaac
     9541 agtttgctga cactatttta gccttgctga acaatccgga aaaattaaac agtattgcaa
     9601 aggaggcaca attgctggca agaagccaat acaacaacaa tataattggt gagcaattgg
     9661 tcaggtttta tcaaaccatc cgataatatt tttgcgctct cttctactcc acttcaattt
     9721 ctacaatttt aatctgatgt ttgtgattgt tttgaattac ctctctgttt tgtatggtag
     9781 tgttggtaaa ttcatgtcta atcaactcaa aaaagccgga agcaaatttt ctatttggct
     9841 cagtgaaaac aattctgcct cctgatttaa ccaatcgttt aaatgcattt acaatggcag
     9901 gaaacattct gctttcgtaa gcaacatcgg cagctaagag caaatcacat ttttcaatat
     9961 catctggtat tttgcgccaa tccaataaaa ttgttgtcgg ttcggatttt acattgagtc
    10021 tccagttggc agatgcaaat tgcaatggag cgtcaacata atcactcatc atcacgctgt
    10081 aacccatttt gccgcaaacc aatgaaggca gtgccagacc acagccaatt tcatgaatca
    10141 gaatatcatt atttagtttc tgagtacaca tatactcagc aagtgcaatt gctgaaggcc
    10201 acaattctgc ccaataggga attcgttcat caatgacatt gtcatcatca ttcttaaggc
    10261 tgcttaactt actatacaat gcatctatgt tggtgattct ataaaaagct atttcctgcc
    10321 ctgtaatatt gattgaatca attacagaag taaccggaat accaaactga aaaacttcag
    10381 gcgatgtcat gttgcaaaat taaaaatcaa caacaaacaa ctgttatctt agcaccatgc
    10441 aaatagtgtt ttcttttatt ctgaatgaaa tcaacagcaa ttatcaattg tatgtggtgc
    10501 aagcaaatta tccctcagaa aaagaaagca gaaaaacatt tcaaaaagag cttaacacaa
    10561 agttgcttaa acaagtaaca aaaaatccat cacttcaatt acactatcag gaaacaggta
    10621 aaccatacat tgatggtaac aatgcaatct ccttttcaca ctccggaaat tatactgcat
    10681 taatgattac acaatcaaaa agtgccggtg ttgacataga aatgacagat agaaatattg
    10741 aagcaggaat tgactatttt ctaaatacta atgaaattgc gtttctttct ctaaaggaaa
    10801 aaaatctgca agcactgaca tgttggtgca ttaaagaagc tgcgattaaa tacttcaatg
    10861 tatcaggaat agactttaaa aactgcatac acattcaacc ttttgaattg ttaaatt
//
LOCUS       DAORQT010000032        10885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_731_length_10885_cov_14.411450, whole genome shotgun sequence.
ACCESSION   DAORQT010000032 DAORQT010000000
VERSION     DAORQT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_10885_cov_14.411450"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PK134_02280"
     CDS             <1..876
                     /locus_tag="PK134_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRB37713.1"
                     /translation="AIQPPDWLLHTNTYLIAFIVFILVVTIVVLFKTNMKLIRMIAPH
                     AFDDEKQTAEAGAVSGVKNRETFMRKVYLRLVDSVPVAKEQDILLDHDYDGIQELDNN
                     LPPWWKWGFYITIIAAVIYMLAYHVTGTGKLQAEEYADELAVAAKQKEERMKNSAENI
                     TEENVTVLVDAAEIAKGKDIYEKNCSACHMADGGGMVGPNLTDSYWLHGGGVKNIFKT
                     ITYGVPAKGMISWQAQLSPKQIQEVTSFIMTLHGTKPAAPKEPQGDEWKDEPAAGATA
                     TDSAAVATTAEAVKK"
     gene            894..2297
                     /gene="ccoG"
                     /locus_tag="PK134_02285"
     CDS             894..2297
                     /gene="ccoG"
                     /locus_tag="PK134_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRB37714.1"
                     /translation="MVENKMEHIDESFRDSIATVTEDGKRAWIYPKKPKGRYYNARTW
                     VSIVFLIIFFALPLIKINGHPFFLLNVVELKFVLFGIAFFPQDFYLFVLAMLTFVVFI
                     ALFTALFGRLWCGWTCPQTVFMEMVFRKIEYLIEGDFMKQKALDKRAWDAEKIRIKTT
                     KHLVFFFIAFLISNTFLMYIIGFDEWKKIITDNPFDHIGGLFATVIFAGVFYGVYARF
                     REQVCIVVCPYGRLQGVMLDPNTVVIAYDYKRGEPRGKLSKDNEEHGDCIDCNQCVQV
                     CPTGIDIRNGTQLECVNCTACMDACDDIMDKIQKPRGLIRYTSENAIKNNAKFKINSR
                     IIGYCVVLLVLFSALVVLLARRSNIETTILRASGLLYQKPDESHISNLYNIALINKTF
                     GNMPIELKIVEPKGEIKWVGNGITHLKEQSVAQSTFFLILPKSEIKKTKTKVVFEIWS
                     EGEKIDRYETSFLGPNN"
     gene            2303..2740
                     /locus_tag="PK134_02290"
     CDS             2303..2740
                     /locus_tag="PK134_02290"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRB37715.1"
                     /translation="MKFNWGHGIALVLIIFASSILVFVYVASKQKNELVTDKYYEKAV
                     AYQQRIDAEKNALNENADVKYNINSGQLMLTLGRNIPNANGTIQFYKPDDATKDFNTS
                     FQVNDTGFYSATIPQLTHGQWKVNVSWNVDSKEYFYEKKIFIP"
     gene            2740..3468
                     /locus_tag="PK134_02295"
     CDS             2740..3468
                     /locus_tag="PK134_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRB37716.1"
                     /translation="MNEYTFYYAALLLGFLGSFHCVGMCGPIALMLPANKLDNRRAAF
                     FGRLFYNIGRMITYMFIGMLFGLLGAAFVLNGFQKQLSIITGIILLLVAIFTLIPSTR
                     KKVLSVSSKMNIPFRGVLRKLFSGKTALHHFGIGVINGFLPCGFVYVATAGAINSGSV
                     LNSVLYMALFGLGTFPIMLLVSYAASWCSFSFSRYVTKATPWISAALALFLIYRGAFM
                     NINADCHKHSHPPTMVECSKPETL"
     gene            3560..6583
                     /locus_tag="PK134_02300"
     CDS             3560..6583
                     /locus_tag="PK134_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB37717.1"
                     /translation="MSHKLLDALMHLFAILANAEKFSLEGRSIVESFLRQQISRAYVL
                     KYLGLFDEYLENIRGKATEGKLKKRLSVNSVKVLRICTDINAELDTRQKHIVLMRLIE
                     FVFTSEKIIDSQEKEFLYTVAEVFHIAPDDVQLCIELACNNAEEKYSDTPKYLVLSSQ
                     DDNSSLNTKTQIISGLNGVFRFLFLKNAGLLFVRYHGDESFTLNGLPLNDDLCYVFSA
                     GSVIRGSKVNTIYYTDIIRLFSNFKQQSLLHFDVQHVYYDFSNGKRGLNDVAFSATSG
                     NLVGIMGGSGAGKSTLLNILNGNNRPTQGKVTVNGIDLHRESHLLEGMIGYVPQDDLL
                     IEELTVYQNLFYNAKLCFGNLTDSEIDERVKALLLNVGLSEASHLRVGNALSTSISGG
                     QRKRLNIALELIRAPEVLFVDEPTSGLSSLDSENVMDLLKQLANHGKLVFVVIHQPSS
                     DIFKMFDKLFVLDIGGYPAYFGNPVDSLLYFKQRASFADADYSECGTCGNINPEQIFS
                     ILESKVMDEFGFPTQQRKLTPADWYQLFKEHNSNSSKSNQRQPVQGSANQKPGWLQQL
                     KVYMTRDMLSKFKNKQYMLITFLEAPVLAIILAWFLRYFNPDGSYVFSENKNLVAFLF
                     MAVIVAIFMGLTVSAEEIIRDRKMLKREKFLNLSRSSYLYSKMMVMFLISAIQTFSFV
                     IIGNLIFGIQSMTFDYWIMLFSVSCFANMLGLNVSSMFDSAVTIYILIPFLIIPQIIL
                     SGVMVKFEDLNPHLTSQRHVPLIGELMASRWAFEALAVNQFSNNPYQKYFFNVDRQMS
                     EATYKKDFWLQKLNDIAEGVSKNKSKNPKVDVELITNELHKEALVNKNFKTAIDFTKA
                     NASEIASQIKPLTDILRNYYIEKYNNASAAKDSFVKKELKHSSVAMLNKQKEKYTNNA
                     LNDLVLNKNDFTILVQDGNSIVQRFQPVYMQPDPTTWFRSPLYVYEKNFFGMKIHTWY
                     VNLFIIWLMSFILMTTLYFDSFRKLIHWVGHSSKR"
     gene            complement(6727..9285)
                     /locus_tag="PK134_02305"
     CDS             complement(6727..9285)
                     /locus_tag="PK134_02305"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRB37718.1"
                     /translation="MLIETITTESQMTTYEYDSVLKASLEYFEGDELAATTWMNKYAM
                     KNKEGKFVERTPHDMHLRMAREFARVEAKYNLLNDGSRTSHLSEYGKKREQLTTEKIT
                     ALFDRFRYVIPQGSVMSALGNPFMIASLSNCIVLPELHDSYGGIFFADQQLAQLYKRR
                     CGAGLDLSTIRPEGMRVSNAAGTTTGAVSFMERFSNTTREVAQLGRRGALMLTLDIAH
                     PDVEKFITVKQDLSKVTGANISVKLSDEFMVAVNENTDFNLRFPVDSTDPYIQKKVNA
                     RELWKTIIACAHRTAEPGIIFWDRQHHYSTSSVYPGFKNVSTNPCSEIAMQGGDSCRL
                     IAINLLSFVDHPFTQKAKFNFTRFYEVTYESQRLMDGLVDLELEAIERILQKIESDPE
                     PDYIKEVEMRTWITLYEAGKKGRRTGLGFTALADALAAMNIAFDSDNALSIVEMMMKE
                     KCRAEFDSSIDMAIERGTFEGFDTAIEDQSEFVNMMKAELPDIYNRMMKYGRRNISIS
                     TVAPTGTLSLLSQTSSGIEPVFMLSYKRRRKVNAEDSKAKTSFVDQSGDAWQEFEVYH
                     HGLKKWMTANNSTDYSKSPYAGSTANEIDWKKRIKMQAVVQKYTTHSISSTINLPENV
                     SAETVGEIYMQAWSAGLKGITVYREGSRSGVLVKKEEKKAEPVITESSAPKRPEILAA
                     KVIRFMNADEKWIAYIGILDNKPYEIFTGKAEDAFSIPAWVSSGWIIKSKNGHGKSRY
                     DFQFNDKEGFKVTIEGLSRSFDKEFWNYAKLISGVLRHGMPIPQVVNLVENLHLYSDS
                     INTWKNGVMRALKQFIPDGTKVNDKVCAECGDKEGLYFEEGCIKCKSCGHSKCG"
     gene            9564..>10885
                     /locus_tag="PK134_02310"
     CDS             9564..>10885
                     /locus_tag="PK134_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRB37719.1"
                     /translation="MKFKTIIEPFRIKMVEPIHLSTEEQRVGYLKQAHYNPFLLNSNQ
                     VLIDFLTDSGTSAMSSDQWSAMMNGDESYAGASSWLRFEKEVQSLTGMPFVLPTHQGR
                     AAERILYGKLGGKGKTFISNTHFDTTRANIEFSGAEAIDIPVKEGLDFASDFPFKGNM
                     DIVLLEKTIAEKGAANIGAVILTVTNNSGGGQPVSMENAKAVADICRKNKVLFVIDCC
                     RVAENSYYIHHNEAGYENKTYRQIAEEMMCLGDAAVMSAKKDALVNMGGFLAVKDADL
                     AESCKQLLIITEGFATYGGLAGRDMEAIAVGLQEVFDPGYLNYRIVSTRYLGENIKSK
                     GVNIIYPVGGHAVYVDAKSLYPHIPPHEYPGQALVCELYRIGGLRSVEIGSVMFGKYD
                     SNGKLIPAPMELVRLAVPRRVYTQSHIDYVIEIFDEIVRTKDKVKGLK"
BASE COUNT         3328 a         1884 c         2142 g         3531 t
ORIGIN      
        1 gctatacaac ctccggactg gctattgcat acaaatactt atctgattgc atttatcgtt
       61 tttattttag ttgtgacgat agttgtgttg ttcaaaacca acatgaaact tatcaggatg
      121 attgcaccac atgcatttga tgatgagaag caaacagcag aagcaggagc agtttcagga
      181 gtgaaaaacc gtgaaacatt tatgcgtaaa gtatatctgc gcttagtgga tagcgttcct
      241 gttgctaaag aacaggatat tcttttagat catgattatg acggcattca ggaacttgac
      301 aacaacctgc cgccatggtg gaaatgggga ttttatataa ccattattgc tgctgtgatt
      361 tatatgttgg catatcatgt taccggcaca ggaaaattgc aagccgaaga atatgcagat
      421 gaacttgctg ttgcagcaaa acagaaggaa gaacgaatga aaaattcagc agagaatatt
      481 acagaagaaa atgtgactgt gttggttgat gcagcagaaa ttgcaaaggg aaaagatatt
      541 tatgaaaaga attgctcagc ttgtcacatg gctgatggtg gcggaatggt tggaccaaac
      601 cttacggata gttattggct gcatggcgga ggcgtaaaga atatttttaa aaccattact
      661 tatggtgttc ctgcaaaggg aatgattagc tggcaggcac aactttcacc aaaacaaatt
      721 caggaagtta cctcttttat tatgacactt catgggacaa agcctgctgc gcctaaagag
      781 ccacagggtg atgagtggaa agatgaacct gctgcaggtg caactgcaac agacagtgct
      841 gctgttgcaa ctactgcaga ggcagtaaaa aaatagtatt aaatctcttt tttatggtgg
      901 agaataaaat ggagcatatt gatgagtcgt tcagagacag catagccact gttactgaag
      961 atggcaagcg tgcatggatt tatcctaaaa aacccaaagg cagatattat aatgcacgaa
     1021 catgggtaag cattgtgttt ctgattattt tctttgcatt accgttgata aagattaatg
     1081 gccatccatt ttttctgctc aatgttgtag agctcaagtt tgtattgttc ggaattgctt
     1141 tttttccaca ggacttttat ttgtttgtat tggccatgct cacctttgtg gtgtttattg
     1201 cattgtttac agctttgttc ggaaggcttt ggtgtggatg gacttgccct caaactgttt
     1261 ttatggaaat ggtgtttcgc aaaatcgagt atttaatcga aggcgatttt atgaaacaga
     1321 aagcactcga taaacgagcc tgggatgcag aaaagatcag aatcaagaca acaaaacatc
     1381 ttgtattctt tttcattgcc tttcttatct ccaatacttt cctgatgtat attatcggat
     1441 ttgatgaatg gaaaaaaatc attaccgata atccttttga ccacattggc ggattgtttg
     1501 caacagtaat ttttgcagga gtattttatg gcgtgtatgc acgctttcgt gaacaggttt
     1561 gtattgttgt ttgcccttat ggccgtttac agggtgtaat gcttgacccc aacactgtag
     1621 taattgctta tgactataaa agaggtgaac caagaggaaa acttagcaaa gacaatgaag
     1681 aacatggcga ttgtattgac tgcaatcaat gtgttcaggt ttgccctaca ggtattgata
     1741 tccgcaatgg cacacagctt gaatgtgtaa actgcactgc atgtatggat gcctgtgatg
     1801 acattatgga taaaatccag aagccacgtg gactcatcag atatacgagt gagaatgcta
     1861 ttaagaataa tgctaagttt aaaatcaatt cgcgcattat cggctattgt gtggtgctgt
     1921 tagttttgtt ctcggcactt gtagttttat tagccagacg atcaaatatt gaaacaacaa
     1981 ttttacgtgc atcgggatta ttgtatcaaa aaccggatga aagccatatc agcaatcttt
     2041 ataacattgc cttgataaat aaaacttttg gtaatatgcc aattgaatta aaaattgttg
     2101 aaccaaaggg agaaatcaaa tgggttggta atggtattac acatcttaaa gaacaatctg
     2161 tggcgcagag tactttcttt ctgattttgc ctaagtctga aattaaaaag acaaaaacaa
     2221 aagtagtttt tgaaatctgg tcagaaggag aaaaaataga tcgctatgaa acttcattct
     2281 taggcccaaa taattaattg aaatgaaatt caactgggga catggtattg cattggtttt
     2341 aataattttt gcctcatcaa tattggtatt tgtttatgtt gcttccaaac aaaaaaacga
     2401 attggtgaca gataaatact acgagaaagc agtggcttat cagcaaagaa ttgatgcaga
     2461 aaaaaatgca ttaaacgaaa atgctgatgt taagtataat attaattccg gtcagctaat
     2521 gttgacatta ggcagaaata taccaaatgc aaatggtact attcagtttt ataagcctga
     2581 tgatgcaaca aaggatttta atacttcatt tcaggttaac gatacaggat tttatagtgc
     2641 cactattcct caattaactc atggtcaatg gaaagttaat gttagctgga atgttgacag
     2701 taaagagtat ttttacgaga agaaaatatt tattccataa tgaacgaata cactttctat
     2761 tatgcagctt tgctactggg ttttcttggt agctttcact gtgtaggaat gtgtggccct
     2821 attgccttaa tgcttccggc aaataagtta gataatcgca gagctgcatt ttttggcaga
     2881 ctgttttaca acatcggcag aatgataacc tatatgttta ttggaatgtt gtttggattg
     2941 ctgggtgctg cttttgtttt aaatggattt cagaaacaac tgagcattat tacaggaatt
     3001 attttgttgt tagttgcaat atttacttta atcccttcaa ccagaaaaaa agttcttagt
     3061 gtatcatcaa aaatgaatat tcctttcaga ggagtgttaa gaaaactttt ttcaggtaaa
     3121 acagccttgc atcattttgg tattggtgtc attaatgggt ttcttccatg tggatttgta
     3181 tatgtggcaa cggcaggagc catcaattca ggcagtgttt taaacagtgt tctgtatatg
     3241 gcattgtttg gtttgggaac ttttccaatt atgcttcttg tttcctatgc agcatcgtgg
     3301 tgtagttttt cattctctcg ttatgtaacc aaagccacac cttggatatc tgctgcatta
     3361 gcattgtttc ttatttacag aggagctttt atgaatatta atgccgattg tcataaacat
     3421 tcacatccac caacaatggt agaatgtagt aagcctgaaa cactttaaaa ttttatttta
     3481 taggattgtt caattgccga tattcggtgg cttaatgata tttgtatatt cgcaacgcta
     3541 cattaaatcg aaaaggtaaa tgagtcataa attattagat gccttgatgc acctttttgc
     3601 cattctggca aatgccgaaa aattttctct cgaaggcaga agtatagttg agtctttttt
     3661 gcgacagcaa atcagccgtg cttatgtttt aaagtacctt ggattgtttg acgagtatct
     3721 tgaaaatatc agaggtaagg caacagaagg aaagttgaaa aaacgacttt cagtaaactc
     3781 ggttaaagtg ctgcgcattt gtacagacat taatgcagaa cttgataccc gccaaaagca
     3841 tattgtttta atgcggctga ttgaattcgt ttttacctct gaaaaaatta ttgacagtca
     3901 ggaaaaagag tttctttata cagttgctga ggtttttcat attgcacccg atgatgttca
     3961 gttatgcatt gaattggctt gtaataatgc agaagagaag tattcagata ctcctaaata
     4021 tttagtgttg tcttcacaag atgataacag cagtttgaat actaaaactc aaattatatc
     4081 agggttaaat ggtgttttcc ggtttttatt tcttaaaaat gccggattgc tttttgtgcg
     4141 atatcacggt gatgaatctt ttacattgaa tggactacca ctcaatgacg atttatgtta
     4201 tgttttttct gcaggctcag tcatcagagg ttcaaaggta aacacaatct attacacaga
     4261 tattatcaga ctgttttcaa attttaagca acagagttta ttgcattttg atgtacaaca
     4321 tgtgtattac gatttttcaa atggtaaacg cgggcttaat gatgttgcct ttagtgcaac
     4381 atcaggaaat cttgttggta ttatgggtgg aagtggcgca ggaaaatcta cattactcaa
     4441 tatcttaaat ggaaacaata gaccaacaca aggaaaagtt actgttaatg gaatagatct
     4501 gcatcgggag tcacatctgc ttgagggtat gataggctat gtgccgcaag acgatttgct
     4561 gatagaagag ttaacagtct atcagaattt attttataat gcaaaacttt gttttggaaa
     4621 tttaaccgat agtgaaattg atgaacgtgt aaaggcactt ttactaaatg tcggcttaag
     4681 cgaagcaagt catctgcgtg ttggcaatgc actcagcaca tctatcagtg gtggtcagcg
     4741 caagcgactc aacattgcat tagagttaat aagagcacct gaagtacttt ttgttgatga
     4801 acccacttca ggactttctt ctctcgactc agaaaatgtg atggacttgt taaagcaact
     4861 tgccaatcac ggcaaacttg tttttgtagt tatacatcag ccatcgagcg atatttttaa
     4921 aatgttcgat aaactttttg ttctcgatat tggtggctac cctgcatact tcggtaatcc
     4981 tgttgactca cttctttatt ttaaacaacg tgcatctttt gctgatgccg attatagcga
     5041 atgtggcaca tgcggcaata ttaaccctga acagattttt tcaattctgg aatcgaaagt
     5101 aatggatgag tttggctttc ctacacaaca acgaaagcta actcctgcag attggtatca
     5161 actgtttaag gaacataata gtaattctag taaatcgaat cagcgacaac ccgttcaggg
     5221 tagtgcaaat caaaagcccg gttggttgca gcaactaaag gtttatatga cacgcgatat
     5281 gctttctaag tttaaaaata agcagtacat gcttattact tttcttgaag caccggtgct
     5341 ggccattatt cttgcatggt ttctcaggta ttttaatcct gatggcagtt atgtttttag
     5401 cgagaataaa aaccttgtag catttttatt catggcagtt atagttgcca tttttatggg
     5461 actcactgta agtgccgagg agattatccg cgacagaaag atgttgaagc gcgaaaagtt
     5521 tcttaatctc agcagaagca gttatctgta ttcaaagatg atggtgatgt ttttaatttc
     5581 tgctatacag acattttctt ttgtcattat cggtaatctt atttttggaa ttcaaagcat
     5641 gacctttgat tattggataa tgcttttctc cgtttcgtgc tttgccaata tgcttggcct
     5701 gaatgtttca tccatgttcg acagtgctgt tacaatttat atcctcattc catttctgat
     5761 tattccgcaa attattttaa gtggtgtgat ggtaaagttt gaagacctga acccgcacct
     5821 gacttcgcaa agacatgtgc cgttaatagg tgaattgatg gcttcgcgat gggcttttga
     5881 agcattagca gtaaatcaat tcagtaataa tccttatcaa aaatatttct ttaatgtgga
     5941 cagacaaatg agcgaagcta cttacaagaa agatttctgg ctgcagaagt tgaatgatat
     6001 tgccgaaggt gtttctaaaa acaaatctaa aaatccgaaa gtagatgttg agcttattac
     6061 taatgaattg cacaaagaag ctttagttaa taaaaatttt aaaactgcaa ttgattttac
     6121 aaaggcaaat gcatcagaaa ttgcatcaca aattaaacct ctgacagata tacttcgtaa
     6181 ttactacata gaaaagtata acaatgcatc tgctgccaag gatagttttg taaagaaaga
     6241 actgaagcat tcatctgtag cgatgttgaa caagcaaaaa gaaaaataca cgaataatgc
     6301 tttgaatgat cttgttttaa ataaaaatga ctttaccatt cttgttcagg atggcaatag
     6361 tattgtgcag cgatttcagc ctgtctatat gcaacccgac ccaacaacat ggtttcggtc
     6421 tccattgtat gtttatgaga aaaatttctt tggaatgaaa attcatacat ggtatgtaaa
     6481 cctttttata atttggttga tgagctttat actgatgaca acactctatt tcgatagttt
     6541 cagaaaactg attcattggg tgggacacag tagcaaacga tgattttttc ttaactgttt
     6601 tttcagaata ttgatataaa attaaatctc tgaaggagac aataaaaaac tgctattaaa
     6661 tcattttttt gttgaatgat ttaatagcag tgcaatttta attactattt tattaagaaa
     6721 gaagtgttat ccgcacttac tgtgtccgca gcttttgcat ttaatacaac cctcttcaaa
     6781 atacaatcct tccttgtcgc cacactctgc acacacctta tcattaactt ttgtaccatc
     6841 aggaataaat tgtttcaatg cacgcattac accatttttc cacgtgttaa tgctgtcgga
     6901 ataaagatgc aaattttcca ccaggttaac tacttgcggt atcggcattc catgtcgcaa
     6961 tacaccgctg ataagtttgg catagttcca gaattcttta tcgaacgagc gcgatagccc
     7021 ttcaatagtt accttaaatc cttctttgtc attaaactga aaatcgtatc tcgatttgcc
     7081 atgtccattt ttactcttga taatccatcc tgaagaaacc catgcaggaa tactgaatgc
     7141 atcttctgcc ttaccggtaa aaatttcata aggtttattg tctaatatac cgatgtatgc
     7201 aatccatttt tcatctgcat tcataaagcg tataactttt gcagcaagaa tttcaggtcg
     7261 ttttggtgca ctcgattctg ttattacagg ttcagctttt ttctcttctt tttttaccaa
     7321 cactccactg cgcgaacctt ctctgtaaac agtaatacct tttaatccgg cactccatgc
     7381 ctgcatataa atttcgccaa cagtttctgc tgaaacattt tcaggtaaat taatagttga
     7441 gctgatagag tgtgtagtat atttctgtac tactgcctgc attttaattc tctttttcca
     7501 gtcaatttca ttggctgtgc ttcctgcata aggactttta ctgtagtcgg tgctgttgtt
     7561 tgcagtcatc cattttttta atccgtgatg atacacttca aactcctgcc aggcatcacc
     7621 ggactgatct acaaaacttg ttttagcctt gctgtcttcg gcattaactt ttcttctgcg
     7681 tttgtaactg agcataaaca caggctcaat acccgatgat gtttgtgata acagacttaa
     7741 tgttcctgta ggagcaactg tgctgataga aatatttctt cttccgtact tcatcattct
     7801 gttgtaaata tccggcaatt ctgctttcat catatttaca aactccgact gatcttctat
     7861 tgcagtatca aaaccttcaa acgttccacg ttctatggcc atatcaatac ttgaatcaaa
     7921 ttctgcacga catttttctt tcatcatcat ttctacaatg ctaagtgcat tgtcggagtc
     7981 aaaggcaatg ttcatggcag caagtgcatc ggctaaagct gtaaatccaa gtcctgtacg
     8041 tcttcctttt ttacctgctt catacaatgt tatccatgta cgcatttcta cttctttaat
     8101 gtagtcaggc tcagggtctg attctatttt ctgaagaata cgttcaatag cctctaactc
     8161 taaatctacc aaaccatcca tgagtctttg tgactcataa gttacttcat aaaaacgtgt
     8221 gaagttaaac ttagcctttt gtgtaaatgg atgatctaca aaactcaata agttgattgc
     8281 aattaatctg caactgtcgc caccctgcat tgcaatttca gagcaagggt ttgttgacac
     8341 gtttttaaat cccggataaa cggaagatgt agaataatga tgttgtctgt cccagaaaat
     8401 aattcccggt tcggcagtac gatgcgcaca ggcaatgatg gttttccata attcgcgggc
     8461 attaactttt ttctgaatat aaggatcagt ggaatcaaca gggaatctca ggttaaaatc
     8521 agtattctca ttaacggcaa ccataaactc atccgataat ttcactgaaa tattggcacc
     8581 tgtaacttta ctcaggtctt gttttacggt aatgaatttt tctacatcag gatgagcaat
     8641 atctagtgta agcatcaagg cacctctgcg tccgagttga gcaacttcgc gcgtggtgtt
     8701 tgaaaaacgc tccataaaag atacagcacc tgtagtagta cctgctgcat tggaaacacg
     8761 cataccttca ggtcttatag tagaaaggtc taagcccgca ccacatctgc gcttatataa
     8821 ttgtgcaagt tgctggtcgg caaaaaatat tccaccataa gaatcgtgta attcaggaag
     8881 aacaatacaa tttgataatg atgcaatcat aaaaggattg cccagagcag acattacact
     8941 gccttgtgga ataacataac gaaagcgatc gaataacgct gtgatttttt cagtagttag
     9001 ttgctcgcgt ttttttccgt attccgacag atgcgaagtt ctgctgccat cgttcagcag
     9061 attatatttt gcttcaactc ttgcaaattc acgagccata cgcaaatgca tatcatgtgg
     9121 tgttcgttct acaaatttcc cttctttgtt tttcatggcg tatttattca tccatgttgt
     9181 agcagcaagc tcatcgcctt caaaatattc tagacttgct ttcaatacgc tgtcatattc
     9241 gtaggtggtc atctgactct cggttgtaat ggtttcaatc aacatatttt atatttctta
     9301 tttgtttaag gcaagcaata atatagacaa aaaaaattct ttcataagga tttgactaat
     9361 atttatcaga cgttgttaac aacccatgtt cactattttt attatcaaca aagtacatca
     9421 aacaaagcgt ttgaattata ggcaattggt acttttgcta acacacaagt aaaaaacaaa
     9481 tgattataat cattagcaaa atttaatcta atcattaagc aatagaaaat ctatttggtt
     9541 cttcgcatct ttcaaattct aatatgaaat tcaagacaat tatcgagcct ttcagaatta
     9601 aaatggtaga acccattcac ctcagcacag aggaacaaag agtaggttat ctgaagcagg
     9661 cacattacaa tccatttcta cttaacagca atcaggtact tatagatttt ctgactgaca
     9721 gtggtacttc tgccatgagt tctgaccaat ggtcggcaat gatgaatggc gatgaatcgt
     9781 atgcaggtgc aagtagttgg ctgcgttttg aaaaagaagt gcaaagcctt acgggaatgc
     9841 cttttgtgtt acctacacat cagggtaggg cagcagagag aattctttat ggaaagcttg
     9901 gtggcaaggg taaaacgttt atcagtaata cacactttga tactacacgt gccaatattg
     9961 agtttagcgg tgccgaagca atagacattc ctgtgaagga agggcttgac tttgcatcag
    10021 attttccttt taaaggaaat atggatatag ttttacttga aaaaactatt gcagaaaaag
    10081 gtgcagctaa tattggtgca gtaatattaa ccgttaccaa caatagtgga ggaggacaac
    10141 ctgtgagcat ggagaatgcc aaggctgttg cagatatttg tcgtaaaaac aaagtgttgt
    10201 ttgttattga ctgctgccgt gttgctgaga acagttatta tattcatcat aacgaagcgg
    10261 gatatgaaaa taaaacctac agacagattg cagaagaaat gatgtgtttg ggtgatgctg
    10321 ctgttatgag tgcaaagaaa gatgcattag taaatatggg tggattttta gcagtgaaag
    10381 atgctgattt ggctgaatcg tgtaaacaat tgttgattat aactgaaggt tttgccacct
    10441 atggcggact tgccggacgc gatatggaag ccatagcagt tggattgcag gaagtgtttg
    10501 atccgggtta tttgaattat cgtattgtaa gcacaagata tttgggcgag aatattaaat
    10561 ccaaaggagt gaatattatt tatcctgtcg gtgggcatgc tgtttatgtt gatgccaaaa
    10621 gtttatatcc tcacatacca ccacatgaat atccgggaca agccttggta tgtgaattgt
    10681 accgtattgg gggtttacgc agtgttgaaa taggatctgt aatgttcgga aagtatgatt
    10741 caaatggtaa gctgattccg gcacctatgg aattggtgcg gcttgctgtt ccaagaagag
    10801 tttatacaca aagtcacata gactatgtga ttgaaatttt tgatgaaata gtgcgtacaa
    10861 aagataaggt taaaggacta aagat
//
LOCUS       DAORQT010000033        10811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_740_length_10811_cov_12.515712, whole genome shotgun sequence.
ACCESSION   DAORQT010000033 DAORQT010000000
VERSION     DAORQT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_740_length_10811_cov_12.515712"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PK134_02315"
     CDS             complement(<1..871)
                     /locus_tag="PK134_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB37720.1"
                     /translation="MNQEKKVIVTPIDFSEQSLIALSQTYNLARLTGSHIRLLHVIDQ
                     DFISHLANSIFSKENYADQVRNNIERRLDELATKVHNEERVSTSTNIRTGKIYDEIVS
                     EANDVDAAFIVMGTQGASTIKKKFLGSNTSRVIKEALCPVITIKGHEHLKGCKDIALP
                     LDLTKETREKVNYAIDMAKLFGAVVHIFSVLETNDEFLVNKLERQLQQVKQFIIESGV
                     ECQGELIPQSDSNVSLSIIEYARKKECDLIIIMTQGETNITDLFIGTAAQEVINNSEI
                     PVLCIRPMVRKDTT"
     gene            1038..1916
                     /locus_tag="PK134_02320"
     CDS             1038..1916
                     /locus_tag="PK134_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRB37721.1"
                     /translation="MNQIPESELILNPDGSVYHLNLLPEDISDVVINVGDPDRVSKVS
                     AFFDSIEVKKQKREFVTHTGHYKGRRISVISTGIGTDNIDIVYNELDALVNIDLKTRT
                     VKSNLTALNLIRIGTSGALQPEIPVDSFVFSTFGLGLDGLLNFYQYTNTPEEQAIIDS
                     FRKHYPNNCKLPESYLTRSSHKLEQALSEGMHKGITASCSGFYAPQGRVLRYQLARTD
                     MIEKLNSFRHDEHKVTNFEMETGAMYGLAKLLGHHCCAVNLIVANRIAQQFSKKYEER
                     MHQLIQTVLDRISAQL"
     gene            1929..2840
                     /gene="gldA"
                     /locus_tag="PK134_02325"
     CDS             1929..2840
                     /gene="gldA"
                     /locus_tag="PK134_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRB37722.1"
                     /translation="MSVSVKNVTKLYGKQRALDNVSFEINTGQVVGLLGPNGAGKSTL
                     MKIISCFIPQNEGKVSVCGFDTLSQSLEVRRNVGYLPEHNPLYPDMFVKEYLQFVGGI
                     YLPAKEAAKRADSMIALTGLTPEYKKKIGALSKGYRQRVGIAQALIHDPAVLILDEPT
                     SGLDPNQLVEIRALIQSIGKQKTVILSTHIMQEVEAICNRVIIINKGKIVADDDTGML
                     RQKAKPVHEITVEFDGKIDAKWLQSIPTVSKVIHHSENTWKVNYSGDDDVRKAVFTMA
                     VSHNVAVLSMQKEESRLEDVFQLLTQG"
     gene            3042..3587
                     /locus_tag="PK134_02330"
     CDS             3042..3587
                     /locus_tag="PK134_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRB37723.1"
                     /translation="MTKNSSEITIIPQEVIINKIYFLRNEKVMLDRDLAELYGVKAIR
                     LREQVKRNLTKFPSHFMFQLTEQEVEIMVSQNAIPSKQHLGGTFPYAFTEHGVLMLAN
                     VLKSERAIQVSIKIIELFVKLREMLVTHTELKLEIERIKKKMDNHDKNIEIVFRYFDE
                     LMEQKINPRKKIGFKLPRKKK"
     gene            complement(3686..4969)
                     /gene="tyrS"
                     /locus_tag="PK134_02335"
     CDS             complement(3686..4969)
                     /gene="tyrS"
                     /locus_tag="PK134_02335"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRB37724.1"
                     /translation="MGFIDELRWRGMLQDIMPGTEELLNKETVTGYIGFDPTSDSLGV
                     GNLVQVTTLMHFQRAGHKPIALVGGATGMVGDPSGKSAERNMLNEETLQHNLNCQKKQ
                     LEKFLDFDCGKNSAEIVNNYDWFRDVKFLDFIRDVGKHITVNYMLAKDSVKNRLENGM
                     TFMEMAYQLVQGYDFYHLYYAKNCKLQMGGSDQWGNIVTGTELLRRKGRVEDAFALTT
                     PLIKKADGTKFGKTESGNLWLDRNKTSPYAFYQFWLNASDEDAKNYIRIFSLKNQQEI
                     EAIESEHLNAPHLRLLQKTLANEITTRVHSQQDCDRAIEISEVFFNGTDKELAQLTES
                     DLQDVFNGLGKNAFEVELQKIQAGIQALDLFAAETTIFTSKGEARKMITANGVSLNKL
                     KFSDPSQIINKEQLIANKFLLIQKGKKNFFLIKAV"
     gene            5092..5334
                     /locus_tag="PK134_02340"
     CDS             5092..5334
                     /locus_tag="PK134_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37725.1"
                     /translation="MAHKQIIIPERFLNYLYHAFKNISNPENHRVKLITRDGQKYRNL
                     QVINGCVLLIERSAPVTSNDIEILQLDMDFVPVKVK"
     gene            complement(5399..6712)
                     /locus_tag="PK134_02345"
     CDS             complement(5399..6712)
                     /locus_tag="PK134_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRB37726.1"
                     /translation="MTLQRLIFIPENKKYLNLFKQLLILVLIWFYAMLTGLSPSVLRS
                     ATMISIIIIAKQLGRDNYMLNTTLASALLLLLINPFSITEVGFQLSYLAVIGIVYLHQ
                     RLVTVLYPTSMLMNQAWQLTSVSLCAQLVTFPLGILYFNQFPNLFLVSNLLVIPISTG
                     IIWVSVGATIISALPYMSSLVFYSWKITFALTWLMNQLLLFFENLPFALISGLYIDIA
                     ETWIIYGIIAFVVWFLLYNYRNALYGALTLTIVFLLAQLWHEWNILQQKNMTVYALKN
                     STAIDLAQARKRLVIAPQQIIEDKSLLRFNINRYWYETGKREMLHIPFYQQPAENKLK
                     EIFNSKIVGNVATIQDGFIQMDSKLFFIPGNWLPRKTPLSKIKVDGLIVTSRFRNKLP
                     TLFKYFDSDLLIIDLSSRTKVDAGKLPVSLQIWDCRKQGAAQVEI"
     gene            complement(6709..8679)
                     /locus_tag="PK134_02350"
     CDS             complement(6709..8679)
                     /locus_tag="PK134_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRB37727.1"
                     /translation="MRHLSHIPVLRILIPFVAGIIVGIFMAVPASLAIALLSSGFIGV
                     LVFHFSKYFSQHYKLRYLFGIVLHLFLFSSGVLLTQLKTKLYDPYHYSQFESAEAFLL
                     RITEPYHIKQKSLKAEAEIEAVFENNKWQPCRGKTLLYFASDAAAKALQYADRIVTNA
                     KPQPIAPPQNPGSFNYKRFLGFHQIYHQFYLKSGRWAAAGKGFSNILLSTAFDLRKKF
                     LNEIQGQILVPQEVAVCSALLIGFNDLLDDDLLSAYASSGALHVLSVSGLHVGLIFVT
                     LQRLIFIPENKKYLNLFKQLLILVLIWFYAMLTGLSPSVLRSATMISIIIIAKQLGRD
                     NYMLNTTLASALLLLLINPFSITEVGFQLSYLAVIGIVYLHQRLVTVLYPTSMLMNQA
                     WQLTSVSLCAQLVTFPLGILYFNQFPNLFLVSNLLVIPISTGIIWVSVGATIISALPY
                     MSSLVFYSWKITFALTWLMNQLLLFFENLPFALISGLYIDIAETWIIYGIIAFVVWFL
                     LYNYRNALYGALTLTIVFLLAQLWHEWNILQQKNMTVYALKNSTAIDLAQARKRLVIA
                     PQQIIEDKSLLRFNINRYWYETGKREMLHIPFYQQPAENKLKEIFNSKIVGNVATIQD
                     GFIQMDSKLFFIPGNWLPRKTPLSKIKVDGFL"
     gene            complement(8813..9043)
                     /locus_tag="PK134_02355"
     CDS             complement(8813..9043)
                     /locus_tag="PK134_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37728.1"
                     /translation="MLSKFIAAIAQTTEQQYMERYWNYRDRYKKWFVKIGREAGESIN
                     LTENDPKGGGGALWTSQPLKVDSFLTGFNCSK"
     gene            complement(9120..9782)
                     /locus_tag="PK134_02360"
     CDS             complement(9120..9782)
                     /locus_tag="PK134_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37729.1"
                     /translation="MKLHACFYLLAAVIALIYGCKKDEPDEPNVSYEDIKPVKDYVYF
                     QLGTYWIYEDNYGNVDSMYVYSAGEGIDTVRKSNGDIEGIYRFFQSKIHSVYDDYNYY
                     IGYNSGWTSFHPTRHQVFMTKTKPGDYVGQIILMEYPPVIGNILYGEKNSIVKIENYY
                     QIYNIGLVNYVSVIKMSNSIDIINNDQRTNYLIAKNVGIIQEELVDSNNVWKVIRYNI
                     VQ"
     gene            complement(9996..10658)
                     /locus_tag="PK134_02365"
     CDS             complement(9996..10658)
                     /locus_tag="PK134_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37730.1"
                     /translation="MKLHACFYLLAAVIALIYGCKKDEPDEPNVSYEDIKPVKDYVYF
                     QLGTYWIYEDNYGNVDSMYVYSAGEGIDTVRKSNGDIEGIYRFFQSKIHSVYDDYNYY
                     IGYNSGWTSFHPTQHKVFMTKTKPGDYVGQIFLTLYPFKIGDWRYWNDSKTTTIDYFQ
                     NYGIDNNSFVSVGKVNVFNDITNNYRQTTNYYIAKNVGIIQEELIDSSNVWKLVRYNI
                     VQ"
BASE COUNT         3590 a         1960 c         1804 g         3457 t
ORIGIN      
        1 gtgtagtgtc ttttctaacc attggtcgga tacataaaac aggaatctct gagttattga
       61 taacttcctg tgctgctgtg ccgataaata aatctgtaat attggtttct ccctgtgtca
      121 tgataataat taaatcacac tctttctttc ttgcgtattc gattatactt aatgatacat
      181 ttgaatcact ttgtggaatc aattcacctt gacattctac tccggattca ataatgaact
      241 gctttacctg ctgcaattgt cgttccagtt tattgactaa aaactcatca ttggtttcga
      301 gcactgaaaa aatgtgcact acagcaccaa aaagtttagc catgtcaatg gcataattca
      361 ccttttcgcg tgtttctttt gtaagatcca atggaagagc aatatcttta caccccttga
      421 ggtgctcatg accttttatg gtaatgacgg ggcataaagc ttctttaatt actcttgaag
      481 tattagagcc aagaaatttc ttctttattg tgcttgcccc ttgtgttccc atcactataa
      541 atgcagcatc aacgtcattg gcttcagata caatttcatc ataaattttt cctgttctga
      601 tgttagtaga ggtcgaaact ctttcttcat tatgcacttt agttgccaac tcatcaagtc
      661 tgcgttctat attatttcga acctgatcgg catagttttc tttagaaaaa atactgttgg
      721 ctaaatggga aataaaatcc tgatcaatta catgtaaaag cctgatatgg ctgccggtca
      781 gtcgtgcaag attataagtc tgtgaaaggg caatgagtga ttgttctgaa aaatctatcg
      841 gggtaactat aaccttcttc tcttggttca taaaaaacag gtgtattaac tatgcaaata
      901 taaaaaaagg aaatatcaaa ttgaataaaa aatgacctcc aacagattat ttaactgtaa
      961 attacatgaa caggtaggtc ttttgacctg atgctttaat atatcttacc ttagcacaga
     1021 aattatttac atttgttatg aatcaaatcc ccgaatcaga gcttattcta aaccccgatg
     1081 gaagtgttta ccatcttaat cttcttcccg aagatatttc tgatgtagtt atcaatgttg
     1141 gcgaccccga tcgcgtatca aaagtgagtg ccttttttga cagcattgaa gtaaaaaagc
     1201 aaaaacgaga gtttgtaacc cataccggtc attataaggg ccgaagaata tctgttattt
     1261 caacaggtat tggcacagat aatatagaca ttgtctataa tgagcttgat gctttagtga
     1321 atattgatct taaaacaaga actgtaaaat ccaatttaac agcacttaat cttattcgta
     1381 ttggcacttc cggtgccctt caaccagaaa ttcctgttga ttcctttgtg ttttcgacat
     1441 tcggtttagg attagatggt ttgcttaact tttatcaata cacaaacacc ccagaagagc
     1501 aggctataat tgatagtttc agaaaacatt atccaaataa ctgcaaacta ccggaatcat
     1561 atctgacacg aagctcacat aaattagaac aagcactttc agaaggtatg cataaaggca
     1621 ttactgcatc ttgttcaggt ttttatgcac cacaaggaag agtgttacgc tatcaattag
     1681 caagaactga tatgattgaa aaattaaatt cattccgcca tgatgagcat aaggttacta
     1741 attttgaaat ggaaacagga gccatgtatg gattggcaaa attattagga catcattgct
     1801 gtgccgtaaa tctgattgtg gcaaacagaa ttgcgcaaca gttttcaaaa aaatatgagg
     1861 agcgtatgca tcaattaatt caaactgtgt tggacagaat tagtgctcaa ctttaaatct
     1921 tccagagtat gtctgtttca gtaaaaaatg taacaaaact ttatggcaag caacgtgcat
     1981 tggataatgt aagttttgaa atcaatacag gtcaggttgt tggtttgctt gggccaaatg
     2041 gcgcaggtaa atcaacgctt atgaaaatta tttcctgttt tattccgcag aatgaaggga
     2101 aggtttctgt ttgcggtttt gatacacttt cacaatcact tgaggttaga cgaaatgttg
     2161 gataccttcc agagcataat ccactttatc ctgacatgtt tgttaaagag tatctacagt
     2221 ttgtgggtgg aatttatttg cctgcaaaag aagctgcaaa gcgtgccgac tctatgattg
     2281 cccttacagg tttaacaccg gaatataaaa agaaaattgg cgcactctca aaaggctaca
     2341 gacaaagagt tggcattgca caagctttaa ttcacgatcc tgcagtactc attcttgatg
     2401 aaccaacttc cgggttagac cccaatcagt tggtagaaat tcgtgcattg attcaatcaa
     2461 taggaaaaca aaaaaccgtt attctttcta cacacattat gcaggaagtt gaagcaatat
     2521 gtaatcgtgt aatcataatc aacaaaggaa aaattgtggc tgatgatgat acaggtatgc
     2581 ttcgtcaaaa agcaaagcca gtacatgaaa tcactgttga gtttgatgga aaaattgatg
     2641 ccaaatggtt gcaatctatt cctacggtga gtaaggttat tcatcattcc gaaaatacat
     2701 ggaaggttaa ttacagcggt gatgatgatg tgcgtaaagc tgttttcact atggcagtca
     2761 gtcataatgt agctgtgctg tcaatgcaaa aagaagaaag caggcttgaa gatgtgtttc
     2821 aactgctaac gcaagggtaa tttaagaatt tacaaaacca atgttacatc agtatctttt
     2881 tattaatgtc ttgttgtgat attgatatac ttaattctga aattttgtaa aacatgttat
     2941 aaattttgta atacctatac acagattctg aaggaagaag taatgtgatt ttttataagt
     3001 acttgttaaa cacctaaatt tacatctctg ataatacaag aatgacaaaa aatagttctg
     3061 aaataacaat aataccacag gaagtaataa taaataaaat ttatttttta agaaatgaaa
     3121 aagtgatgct tgacagagat ttagctgaat tatatggtgt taaagccatc agactacgag
     3181 aacaagtaaa acgaaatcta acaaaatttc cttcccattt tatgtttcag ttaactgaac
     3241 aagaggttga aattatggta tcgcaaaatg cgataccttc aaaacaacat ttaggtggta
     3301 cttttcctta tgcatttaca gaacatggtg tactaatgtt agctaatgtc ttaaagagtg
     3361 agagagctat acaggtaagt attaaaataa ttgagttgtt tgtgaaactt cgtgaaatgt
     3421 tggttacaca tactgaactg aagttagaaa tagaacggat aaaaaagaaa atggataatc
     3481 atgataagaa tattgaaatt gtgtttagat attttgatga attgatggaa caaaaaataa
     3541 atcccagaaa gaaaatagga tttaaactac caaggaaaaa gaaataacca catataaatc
     3601 ctgcctgtta aaaccaaata acagataatt taaaaagatt gttgctaaat gcttagaaaa
     3661 gtaacaaata cttatgctaa gaagtttaaa cagccttaat caggaaaaaa ttcttcttac
     3721 ctttctgaat cagtaaaaac ttatttgcaa tcagttgttc tttattgata atttgtgaag
     3781 ggtcagaaaa tttcaattta ttcaatgaga caccatttgc agtaatcatt tttcttgctt
     3841 cacctttact tgtaaaaatt gttgtctctg ctgcaaataa atcaagtgcc tgaatacctg
     3901 cctgaatttt ttgcaactca acttcaaatg catttttgcc taatccgtta aaaacatcct
     3961 gcaaatcaga ctctgttagt tgcgcaagtt ctttatctgt tccattaaaa aatacttcag
     4021 aaatttctat agctctgtca caatcctgtt gtgaatgcac acgagtagtt atttcattag
     4081 ctaatgtttt ctgaagcaat ctcaaatgag gtgcatttaa atgttcagac tctattgctt
     4141 ctatctcctg ctgattcttt aaagaaaata tacggatata attttttgca tcttcatcgc
     4201 ttgcattcag ccagaactga taaaaagcat aaggagatgt tttatttctg tcaagccaca
     4261 aatttccact ctctgtctta ccgaatttag taccatctgc tttttttatc agtggtgttg
     4321 ttaaagcaaa tgcatcttca acacggcctt ttctgcgaag caattcagtt cctgtaacaa
     4381 tatttcccca ctgatcgctg ccacccattt gcagcttaca gttttttgca taatacaaat
     4441 gataaaaatc gtagccctgc actaactgat atgccatttc cataaatgtc attccattct
     4501 ctaatctgtt ttttacagaa tcttttgcca gcatataatt aacagtaatg tgtttcccca
     4561 catcacgaat aaaatcaaga aactttacat ccctgaacca atcataatta ttaactattt
     4621 ctgctgaatt ttttccacaa tcaaaatcca gaaatttttc taattgtttt ttctgacaat
     4681 ttaaattgtg ctgcagtgtt tcttcgttta acatatttct ttcagcactc tttccgcttg
     4741 gatcgccaac catacctgtt gcaccgccaa ccaaggcaat tggtttatgt cctgctcttt
     4801 gaaaatgcat caatgtagta acctgcacta aattgcctac gcctaatgaa tcgctggtag
     4861 gatcaaatcc tatatatccg gtaaccgttt ctttattcaa taattcctct gtaccgggca
     4921 taatatcctg caacatgccg cgccatctta gttcgtcaat aaatcccata tctgattttt
     4981 agtgccggca aagataatca tacagtgctt tactgctatc agttacaaag ggtaaaattc
     5041 atgcaacctt tttatctgta tcaccgtaca acacgcttaa acattatcaa tatggcacat
     5101 aagcaaatca ttatccccga aagatttcta aactatttat atcatgcttt taaaaatata
     5161 agcaatcctg aaaatcacag agtaaaactt attactcgtg acggacagaa gtatcgaaat
     5221 ctgcaggtta ttaatggctg tgtattgtta attgaaagat ctgcacctgt tacctcaaac
     5281 gatattgaga ttcttcaact tgatatggat tttgttcctg taaaagttaa ataatatttg
     5341 atgttggcac agccggtagt tccttttggg gattgccggt tttttatttg ctgctccctc
     5401 aaatttctac ttgtgctgcg ccttgttttc tgcaatccca gatttgtaaa ctgacaggca
     5461 atttacctgc atcaactttt gttcggctgc ttaaatcaat aatcaacaaa tcagaatcaa
     5521 aatatttaaa gagcgtaggc agtttatttc tgaaacgtga ggtaacaatc aatccatcaa
     5581 ctttaatttt acttaatggt gtttttcgcg gcagccagtt acccggaata aaaaataatt
     5641 tactatccat ctgaataaat ccatcctgaa tagtagcaac atttcctaca atcttactgt
     5701 taaaaatttc tttcaactta ttttctgcag gctgctgata aaatgggatg tgaagcattt
     5761 ctctttttcc tgtttcatac caatagcggt taatattaaa acgtaacagg cttttatctt
     5821 cgattatctg ttgtggtgct atgaccaacc tctttcttgc ctgtgccaga tcaattgctg
     5881 tgctgttttt taaagcatat actgtcatgt ttttctgttg cagaatattc cactcatgcc
     5941 aaagttgagc caacagaaaa actattgtta acgtcaatgc tccataaagt gcattcctgt
     6001 agttgtaaag caaaaaccaa actacaaaag cgataatgcc atagataatc catgtttctg
     6061 caatatcaat atacaatccg ctaatcaatg caaatggaag gttttcaaaa aacaacaata
     6121 actgattcat taaccaagtg agtgcaaaag ttattttcca agaataaaat acaagtgatg
     6181 acatgtaggg caatgctgat ataattgtag caccaacaga cacccagata attcctgtag
     6241 agataggtat gactaaaaga ttagatacta aaaacaaatt cgggaactga ttaaaataaa
     6301 gtattcctaa cggaaatgtt accagctgcg cacataatga cactgaagtc agttgccatg
     6361 cctgattcat gagcattgaa gtaggataaa gaaccgtaac caatcgctga tgcagatata
     6421 caattccgat aacagcaagg tatgaaagtt gaaaaccaac ttctgtaatt gaaaatgggt
     6481 taatcaacaa caacaataaa gcagaagcca gtgtagtgtt aagcatgtag ttatcacgac
     6541 caagttgctt tgcaataatg atgatagaaa tcatggttgc tgaccgtaaa acggaaggag
     6601 aaagtcctgt tagcatggca taaaaccata tcaaaaccag aatgagtaat tgtttaaaaa
     6661 gattcagata cttcttattt tcaggaataa aaatcagcct ttgcagggtc acaaaaatcc
     6721 atcaacttta attttactta atggtgtttt tcgcggcagc cagttacccg gaataaaaaa
     6781 taatttacta tccatctgaa taaatccatc ctgaatagta gcaacatttc ctacaatctt
     6841 actgttaaaa atttctttca acttattttc tgcaggctgc tgataaaatg ggatgtgaag
     6901 catttctctt tttcctgttt cataccaata gcggttaata ttaaaacgta acaggctttt
     6961 atcttcgatt atctgttgtg gtgctatgac caacctcttt cttgcctgtg ccagatcaat
     7021 tgctgtgctg ttttttaaag catatactgt catgtttttc tgttgcagaa tattccactc
     7081 atgccaaagt tgagccaaca gaaaaactat tgttaacgtc aatgctccat aaagtgcatt
     7141 cctgtagttg taaagcaaaa accaaactac aaaagcgata atgccataga taatccatgt
     7201 ttctgcaata tcaatataca atccgctaat caatgcaaat ggaaggtttt caaaaaacaa
     7261 caataactga ttcattaacc aagtgagtgc aaaagttatt ttccaagaat aaaatacaag
     7321 tgatgacatg tagggcaatg ctgatataat tgtagcacca acagacaccc agataattcc
     7381 tgtagagata ggtatgacta aaagattaga tactaaaaac aaattcggga actgattaaa
     7441 ataaagtatt cctaacggaa atgttaccag ctgcgcacat aatgacactg aagtcagttg
     7501 ccatgcctga ttcatgagca ttgaagtagg ataaagaacc gtaaccaatc gctgatgcag
     7561 atatacaatt ccgataacag caaggtatga aagttgaaaa ccaacttctg taattgaaaa
     7621 tgggttaatc aacaacaaca ataaagcaga agccagtgta gtgttaagca tgtagttatc
     7681 acgaccaagt tgctttgcaa taatgatgat agaaatcatg gttgctgacc gtaaaacgga
     7741 aggagaaagt cctgttagca tggcataaaa ccatatcaaa accagaatga gtaattgttt
     7801 aaaaagattc agatacttct tattttcagg aataaaaatc agcctttgca gggtcacaaa
     7861 aatcaagcct acatgcaatc cggatacact caacacatgt aatgcaccgg agcttgcata
     7921 ggccgacaat aaatcatcgt caagcaaatc attaaagcca attaacaatg cagaacagac
     7981 cgctacttcc tgcggcacta aaatttgtcc ttgtatttca ttcaaaaact ttttgcgtaa
     8041 atcaaaagct gtactcaaaa gaatattaga aaatccttta ccggcagcag cccatcgccc
     8101 cgactttaaa tagaactgat ggtatatctg atgaaaacca agaaatcttt tgtagttaaa
     8161 tgaccccgga ttttgtggag gagcaattgg ctgtggcttt gcattggtaa caatcctatc
     8221 agcatactgc aatgcctttg ctgctgcatc ggatgcaaaa tacaacaatg tttttcctct
     8281 gcatggctgc catttattgt tttcaaagac agcttctatt tctgcttctg cttttaaaga
     8341 tttttgtttg atgtgatatg gctcggtgat acgaagtaaa aaagcttcag cgctttcgaa
     8401 ttgcgaatag tgataggggt cgtaaagttt cgtctttaat tgtgtgagta aaacgcctga
     8461 agagaataaa aacaaatgca atactattcc gaaaaggtag cgaagtttat aatgctgact
     8521 aaaatatttt gagaaatgaa aaactaaaac tccgataaaa cctgaagaca acaaagcgat
     8581 tgccaaagat gcaggcacag ccatgaatat gcctacaatt ataccggcaa caaaaggtat
     8641 taaaattctt aaaacaggaa tatgcgatag gtgacgcata actgtgcggt ttggtgtaaa
     8701 attaagaaaa ttaattctga attgtagaat ttaatcgaga ataatttatc ttacttaagg
     8761 ctttttacag cagggcctgc ttccgtttaa attcatcctg caatattcta tatcatttac
     8821 tacaattaaa acctgtcaaa aagctgtcca ccttcaaagg ctggcttgtc cacaatgcgc
     8881 cacctcctcc cttggggtcg ttttcggtca ggttaatgct ttcgcccgcc tcacgcccta
     8941 tttttacaaa ccatttcttg tatcggtctc tgtagttcca gtagcgttcc atgtattgct
     9001 gctctgttgt ttgtgcaata gcggcaatga attttgaaag cacagcagca gcatggctat
     9061 ggttttccag ttgattttaa ttttcatact atttggtttt tagtggttat taataaattt
     9121 tattgtacta tgttatacct tattaccttc catacattat tgctatcaac cagttcttcc
     9181 tgaataatac ctacgttttt ggcaattaaa taatttgtcc tctgatcatt attaattata
     9241 tctattgaat tcgacatttt aataacagat acataattta ctaacccaat attatatatt
     9301 tgataatagt tttcaatttt tacaatagaa tttttctcac catataatat attaccaatg
     9361 acaggaggat attccattaa aattatttgc cctacataat ctccgggctt tgttttagtc
     9421 ataaagactt gatgtctggt tggatgaaaa gatgtccagc ctgagttata accaatataa
     9481 taattgtaat catcataaac actgtggatt ttactttgaa aaaagcggta aataccttca
     9541 atatcaccat ttgattttct tacggtatca atgccttctc cggcagaata tacatacatg
     9601 ctgtcaacgt ttccgtaatt atcctcatat atccagtaag tgccaagctg gaaataaaca
     9661 taatccttaa caggcttgat gtcttcatag cttacatttg gctcatcagg ctcatctttt
     9721 ttgcagccgt aaatcaaagc aataacagca gcaagcaaat aaaaacaggc atgcagcttc
     9781 atgagtgttt tcttgaaggt gtaaatatat gaggcttgag ttaaaacaaa tatttttaaa
     9841 aattatttat gcgtatgctt tgcacaaaca gcggcaatga attttgaacg cacagcaaca
     9901 gcatggctat ggttttccag ttgattttaa ttttcatact atttggtttt tagtggttaa
     9961 atgaatctat caatagtaaa tattaaagat taggtttact gaacaatatt atatctgact
    10021 aacttccaca cattgctgct gtctatcagt tcctcttgta tgattcctac attctttgca
    10081 atataataat ttgttgtttg tctataatta ttcgtaatat cattaaaaac attaaccttt
    10141 ccaaccgaaa caaaactatt attgtcaata ccataatttt ggaaataatc aatagtagtt
    10201 gttttagaat cattccagta tctccaatca cctattttaa agggatataa ggttaaaaaa
    10261 atttgcccta cataatcccc cggctttgtt ttagtcataa aaaccttatg ttgggttgga
    10321 tgaaaagatg tccagcctga gttataacca atataataat tgtaatcatc ataaacactg
    10381 tggattttac tttgaaaaaa gcggtaaata ccttcaatat caccatttga ttttcttacg
    10441 gtatcaatgc cttctccggc agaatataca tacatgctgt caacgtttcc gtaattatcc
    10501 tcatatatcc agtaagtgcc aagctggaaa taaacataat ccttaacagg cttgatgtct
    10561 tcatagctta catttggctc atcaggctca tcttttttgc agccgtaaat caaagcaata
    10621 acagcagcaa gcaaataaaa acaggcatgc agcttcatga gtgttttctt gaaggtgtaa
    10681 atatatgagg cttgagttaa aacaaatatt tttaaaaatt atttatgcgt atgctttgca
    10741 caaacagcat ccctggcgca agtcttcgga cttgtgccca cctatcaata aagaatgcaa
    10801 tgcaataatt t
//
LOCUS       DAORQT010000034        10774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_744_length_10774_cov_6.383898, whole genome shotgun sequence.
ACCESSION   DAORQT010000034 DAORQT010000000
VERSION     DAORQT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_10774_cov_6.383898"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PK134_02370"
     CDS             <1..961
                     /locus_tag="PK134_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRB37731.1"
                     /translation="QYTSDVEAIKGLVNSYQKLRTEIGKVIVGQDEVVRDVLISIFSK
                     GHCLLVGVPGLAKTLLVNTISQALGLSFSRIQFTPDLMPSDITGIEILDENRHFKFIK
                     GPVFANIILADEINRTPPKTQAALLEAMQERSVTVSGKRYELPNPFFVLATQNPIEQE
                     GTYPLPEAQLDRFMFNVFLDYPSFEEEVLVVKNTTGDKLGKAEKVLDENEITFFQDLI
                     RKIPVPDNVLHYAVGLTAKTRPGAENKSAMINDYVAWGAGPRASQFLVIGAKCHAAIN
                     GKFSPDIEDVKAVAVPILRHRLVRNYKAEADGVSVEKIIDSIL"
     gene            1113..2243
                     /locus_tag="PK134_02375"
     CDS             1113..2243
                     /locus_tag="PK134_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37732.1"
                     /translation="MRTLKLIGCGLLMTIIASCSSGDSSEQETDEGTETGTTTQATER
                     NFSGKNSNWAKLVAVENRDANGNLVSTIPLPSNWKIHNSRQQGDPTFTGPDGIVVKDY
                     PLQMFNYPFDQNTQYIYSQSGQQVRDFPGVEQVIQQDLIPVCRNQGLSFIKYYELPEV
                     SRIEKWYSDQLFQAVPMDKKTFAIGTDWQTADGNPYFLLVHFSISTTAQLRMWSYFVS
                     SLEADKDVFEKAKKQLLFSISNAHYELEPIMAYNRSEAEKAGRSWAQFNARMAQNQAN
                     FEASQRAHINKTNAINDAIMSGYRERNAASDRMQEQRIDGIYERTNVVDESGQKYKVE
                     SGYNQYWMNNDGEYISTDKVDYNPNLDDNMNDQKWQELKEIK"
     gene            complement(2252..3202)
                     /gene="ftsY"
                     /locus_tag="PK134_02380"
     CDS             complement(2252..3202)
                     /gene="ftsY"
                     /locus_tag="PK134_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRB37733.1"
                     /translation="MAFFGLFSKKDKESLDSGLNKTRENFFTKITKAVIGKSTVDDEV
                     LDNVEEILVSSDVGVDTTLKIVKRIQDRVARDKYVGTDQLNTILKEEIAALLGENKSA
                     TVANLIDESKKPYIIMVVGVNGVGKTTTIGKLAYQFKQSGKNVLLGAADTFRAAAVDQ
                     LNIWAERVQVPIVSQGMNADPASVAFDTVKSGVAQNSDVVIIDTAGRLHNKVNLMNEL
                     TKIKRVMQKVVSDAPHEILLVLDASTGQNAIEQARQFTAATEVNALALTKLDGTAKGG
                     VVIGISDEFKIPVKYIGVGEKMTDLQAFDRVSFVDSLFAK"
     gene            complement(3285..3437)
                     /locus_tag="PK134_02385"
     CDS             complement(3285..3437)
                     /locus_tag="PK134_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRB37734.1"
                     /translation="MAKKVVATLKTGKGKEFSKVIKMVKGANGAYSFKEEIVHNDHVK
                     DFLKEK"
     gene            complement(3455..3637)
                     /gene="rpmG"
                     /locus_tag="PK134_02390"
     CDS             complement(3455..3637)
                     /gene="rpmG"
                     /locus_tag="PK134_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRB37735.1"
                     /translation="MAKKGNRVQVIMECTEHKNSGMPGTSRYITTKNKKNTTERLELK
                     KYNPILKKVTLHKEIK"
     gene            complement(3650..3883)
                     /gene="rpmB"
                     /locus_tag="PK134_02395"
     CDS             complement(3650..3883)
                     /gene="rpmB"
                     /locus_tag="PK134_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRB37736.1"
                     /translation="MSRICDLTGKTSIVGNNISFSNRKTKRRFNPNLQTKKFFIPETG
                     EWVTLKVSASAIRTINKKGISACINSLIKKGHI"
     gene            complement(4032..5291)
                     /locus_tag="PK134_02400"
     CDS             complement(4032..5291)
                     /locus_tag="PK134_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB37737.1"
                     /translation="MTSVQSHLQQLNGNRIMGFSSSKKYFSWALALLLMAGCSSSRKT
                     AQMPPPAPAPKPVEQPVKKPEVVHEKTEKKEFNIGLMLPLNLEQQMQVFDDTSGSAMP
                     DYNNLSNLQFAEGALLAADSLKQLGINIKLNIAETPPDSFALWRLWRQNNFVKCEAVI
                     ASVPSSFNPAVARLSANNKQSLILTQPSNINFKANSEHVYMLSPSTYTQCMVAAKEIA
                     TRFPDSKYVLLARDVKPDKDIAQIFKDHLPAKRLKHFITSTVNPDSVCKILKTENAVL
                     IVTSSAEAYVNTILNQVNACIEKPVFAIGLPTWENFESIDFSILKNLRIAFFTSTYLD
                     YHAASTKSFRNNFINRYKTEPLLNAFQGYEAVMEYVKSNTSHGTLRAGYLPTLNIKYV
                     KDAANGIKENQGINLIEIKDYSLIPLE"
     gene            complement(5248..6774)
                     /gene="guaA"
                     /locus_tag="PK134_02405"
     CDS             complement(5248..6774)
                     /gene="guaA"
                     /locus_tag="PK134_02405"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRB37738.1"
                     /translation="MHQTILIIDFGSQYTQLIARRLRELNVYCEIHPYNHLPEMSDSI
                     KGVIFSGSPFSVRDSQAPLIDTKSFFGKTPVLGLCYGAQLLAHQHQGEVVASKIREYG
                     RAILKINFDDEIFNDVSDNSQVWMSHADTIRSAGNDFEVIAQTDDVAVAAFRHKHLAV
                     YGFQYHPEVYHSTDGKKMLANFVYNICGCRGDWTPSSFVDETVAQLQDKLKNDKVILG
                     LSGGVDSSVAALLLHKAIGKNLYCIFIDNGLLRKNEAQHVMEVFKPLGLNIKCVDAAT
                     VFYDALKNISDPEQKRKTIGRVFIEVFDKEAHAISDVKWLAQGTIYPDVIESVSVNGP
                     SATIKSHHNVGGLPEKMNLKIVEPLRSLFKDEVRKVGKEMGLKEELLNRHPFPGPGLG
                     IRILGEISAEKVKMLQEADHVFISLLQKHNLYQKVWQAGAIFLPVNSVGVMGDERTYE
                     NAICIRAVTSTDGMTADWAHLPYEFLSIVSNEIINKVKGINRVVYDISSKPPATIEWE
                     "
     gene            6922..7150
                     /locus_tag="PK134_02410"
                     /pseudo
     CDS             6922..7150
                     /locus_tag="PK134_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218453.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
     assembly_gap    7151..7207
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7208..8791
                     /gene="thrS"
                     /locus_tag="PK134_02415"
     CDS             <7208..8791
                     /gene="thrS"
                     /locus_tag="PK134_02415"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRB37739.1"
                     /translation="KIEDKMIELARKNNVYIRKEVPKQEAIAYFTEKNDEYKLDLLQN
                     LEDGNITFYTQGNFTDLCRGPHIPHTGYIKAVKLMNLAGAYWKGDEKNKQLTRVYGIT
                     FPKQKELDEYLAMLEEAKKRDHRKLGKELDIFMFDDEVGPGLPLWLPNGGVLIEQLEN
                     LAKRTENEAGYKRVRTPHLAKESMYLTTGHLPYYAESMFPPMEMDGEKYYLKPMNCPH
                     HHKIFGNLNASYRDLPIRLAEYGTCYRYEQSGELFGLMRVRCLQMNDAHIYCTEEQFA
                     SEFKAVNDMYLKYFKIFGIEKYIMRFSTHSPEKLGKKYVNEPELWLKTEDMVRRVLIE
                     SNIPYIEVPDESAFYGPKIDVQVWSAIGREFTLATNQVDFAVPARFGLTYRDADNTMK
                     TPLCIHRAPLGTHERFIGFLIEHYAGNFPLWLAPMQVAILPISEKFNDFAKSVSQLLN
                     NYDIRALVDERGEKIGRKIRDAEVKKVPFMLIVGEKEMNEKLVSVRKHGEGDKGSMTP
                     EAFCQLVKKEIEDQLLINK"
     gene            8894..9391
                     /gene="infC"
                     /locus_tag="PK134_02420"
     CDS             8894..9391
                     /gene="infC"
                     /locus_tag="PK134_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRB37740.1"
                     /translation="MNGRIHSPQVRLVGDNVEPRIYDTQTALDMAQDMGLDLVEISNT
                     SNPPVCKIVDYQKFLYERKKKDKEQKAKAAKSVVKEIRFGPNTDEHDFNFKLKHAIKF
                     LEEGAKVKAYVHFKGRSIAYKEKGEIILLKFAQELEQYAKVEMLPKLEGNRMFLHLAP
                     LAKKK"
     gene            9426..9623
                     /gene="rpmI"
                     /locus_tag="PK134_02425"
     CDS             9426..9623
                     /gene="rpmI"
                     /locus_tag="PK134_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRB37741.1"
                     /translation="MPKMKRNSSAKKRFSVTGTGKIKRKHAFKSHILTKKSTKRKRAL
                     TNAAIVHKSDEFNVKRMLSIA"
     gene            9708..10052
                     /gene="rplT"
                     /locus_tag="PK134_02430"
     CDS             9708..10052
                     /gene="rplT"
                     /locus_tag="PK134_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRB37742.1"
                     /translation="MPRSVNSVASRARRKKLLNRAKGNWGARGNVLTVAKHTVEKGLQ
                     YAYRDRKTKKRNFRGLWIQRINAEVRNYDMSYSEFMGKLHKAGNQLNRKVLADLALNN
                     PDAFKAIVESVK"
     gene            complement(10307..10585)
                     /locus_tag="PK134_02435"
     CDS             complement(10307..10585)
                     /locus_tag="PK134_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37743.1"
                     /translation="MKKLLLVFALGLGLTFAQAQTVATTNNKATTTHAAKATSVKSEK
                     STAADHTKKDGTPDKRFKENKNVKAQGPKKADGTPDMRYKSNKPAAKK"
BASE COUNT         3420 a         2050 c         1985 g         3262 t
ORIGIN      
        1 acaatataca tcagatgttg aagctataaa aggattagtc aacagttatc agaaacttcg
       61 aacagaaatc ggaaaagtaa ttgtcgggca ggatgaagtt gtacgtgatg tattaatttc
      121 tattttcagc aaaggacact gccttcttgt tggtgtaccc ggtttagcaa aaacgctttt
      181 agtaaacaca atttcacaag cattaggact ttcattcagc cgcattcaat ttacgcctga
      241 tttaatgcca agtgatatta caggtattga aatattagac gaaaacagac actttaagtt
      301 tatcaaaggt ccagtctttg ccaatataat tcttgcagat gagattaacc gtacgcctcc
      361 taaaacacag gcagcacttc ttgaagccat gcaggaaagg tctgtaaccg tttccggaaa
      421 acgttatgag ttgcctaatc cattttttgt tctggcaaca caaaatccaa tagaacagga
      481 gggtacttat ccccttcctg aagctcagct cgaccgattt atgtttaatg ttttcctcga
      541 ctatccctcg tttgaggaag aagttctggt tgtaaaaaac acaaccggtg ataaacttgg
      601 caaagcagag aaagtgcttg acgaaaatga aattactttt tttcaggatc tgattcgcaa
      661 aatccctgta ccagacaatg tattgcacta tgcagtaggt cttactgcaa aaacacgtcc
      721 gggagctgaa aacaaatctg caatgattaa tgattatgtt gcatggggag caggtccgcg
      781 tgcatcacag tttttagtta taggtgctaa atgtcatgct gcaatcaacg gtaagttttc
      841 tcctgatatt gaagatgtaa aagcagttgc cgttccaata cttcgtcatc gtttagtgcg
      901 taattataag gcagaagctg atggtgtttc tgttgaaaaa atcattgaca gcatcttgta
      961 gttttctatt tttcgttaat ctcctcctct tttcattctc ttcggaaaac atcaaatccg
     1021 cgatttctga ctcatagcac caatcaccat aattggtgat tggtgattat ttctgatatt
     1081 attagtttta cattttatta aactaataaa agatgaggac attaaaattg attggctgcg
     1141 gattactgat gacaattatt gcatcttgca gcagtggaga ctcttccgaa caggaaactg
     1201 atgaaggaac agaaactggt acaacaacac aggcaacaga aagaaatttc tcaggtaaaa
     1261 atagcaattg ggcaaaatta gttgcagttg aaaatcgcga tgctaatggc aatttggtgt
     1321 ccacaatacc attaccttca aattggaaaa tacataacag cagacaacaa ggcgatccta
     1381 cttttacagg tcctgatgga attgtagtta aagattatcc gcttcagatg tttaattacc
     1441 catttgatca aaacacacag tatatctata gtcaaagtgg acaacaggtt agagatttcc
     1501 caggtgtaga acaggtcatt caacaagatt taattcctgt ttgccgcaat cagggacttt
     1561 cattcataaa atattatgag ttgccggaag tatcgcggat tgagaaatgg tatagcgatc
     1621 agttatttca ggcagtacca atggataaaa aaacatttgc tattggaaca gattggcaaa
     1681 ctgctgatgg caatccctat tttttacttg tacatttttc tatcagcacc actgcgcaac
     1741 tgcgaatgtg gagttatttt gtttccagcc tggaagcaga caaggatgtt tttgagaaag
     1801 caaagaagca actcttgttt tcaatttcaa atgcacatta cgaactggaa cccattatgg
     1861 cttacaacag atcggaagca gaaaaagccg gaagaagttg ggctcagttt aatgctagga
     1921 tggcacaaaa tcaggctaat tttgaagcat cacaacgtgc acacatcaat aaaacaaatg
     1981 ctataaacga tgccattatg agtggctacc gcgaaagaaa tgctgcatca gacagaatgc
     2041 aggaacaacg tattgatgga atttatgaaa gaaccaatgt tgtggatgaa agcggacaga
     2101 agtataaagt tgaaagtggt tacaatcaat actggatgaa taacgatggt gaatatatca
     2161 gtacggataa ggttgattat aaccccaacc ttgatgataa tatgaatgac caaaaatggc
     2221 aggagttaaa agaaattaaa tagcagtaat gctattttgc aaaaagagaa tcaacaaaac
     2281 taaccctgtc aaaagcctgt aagtcggtca tcttctctcc tactccaata tattttacag
     2341 gtattttaaa ctcatcagaa ataccaatca ccacaccacc ttttgcagtg ccatctaatt
     2401 ttgtcagcgc aagtgcatta acttctgttg cagcagtaaa ctgtcttgcc tgctcaatag
     2461 cattctgacc tgttgaagca tcaagcacca ataaaatttc atgaggtgca tcggacacaa
     2521 ctttctgcat tacacgtttt attttagtca actcattcat gagattaact ttattgtgca
     2581 agcgacctgc tgtatctatt ataaccacat cgctgttctg agccacacct gacttaacag
     2641 tatcaaatgc aaccgaagcc ggatcggcat tcattccttg cgaaacaata ggcacctgaa
     2701 cacgttctgc ccaaatgttt aactgatcta ctgcagcagc acgaaaagtg tcggccgcac
     2761 ccagtaacac attttttcct gattgtttaa actgatatgc caatttacca atagtcgttg
     2821 tcttgcccac gccatttaca cctacaacca ttatgatata gggtttctta ctttcatcaa
     2881 ttaaattggc cacagtagcc gatttgttct cgccaagtaa tgcagctatt tcttctttta
     2941 aaattgtatt caactgatcg gttcccacat atttatcacg tgctaccctg tcctgaattc
     3001 gttttacaat ttttaaagtt gtatccactc caacgtcaga cgagactaaa atctcctcta
     3061 cattatcgag cacttcatca tcaaccgttg atttaccaat tactgctttg gtaatttttg
     3121 taaaaaaatt ctcacgtgtt ttatttaatc cgctgtcgag tgattcctta tcttttttcg
     3181 aaaacagtcc gaaaaatgcc atattatgat tcttttatta tagtattaaa acaaaaacag
     3241 ccttcttcct tagggaagaa agctgtcatt cgatttcata aatattattt ctctttcaaa
     3301 aaatctttta cgtgatcgtt atgcacaatc tcttctttga atgaataggc tccattggca
     3361 cctttcacca tttttatcac cttggaaaac tcttttcctt taccggtttt aagtgttgca
     3421 actacttttt tagccatttt atttaagctt taaattattt aatttcttta tgaagagtaa
     3481 cttttttcag aatggggttg tattttttca actccaaacg ctctgtagtg ttcttcttgt
     3541 tctttgttgt gatataacgt gatgttccgg gcataccgct gttcttatgc tctgtgcact
     3601 ccatgataac ctgaactctg tttccttttt ttgccatgat gctaaaagat taaatgtgtc
     3661 ctttttttat cagactgttt atacaggctg aaattccttt tttattgatg gtacggattg
     3721 cagaagcaga tacttttaaa gtaacccact caccggtttc cggaataaaa aacttttttg
     3781 tttgcagatt tggattgaat ctgcgctttg ttttacgatt ggaaaaagaa atattgtttc
     3841 caacaatact tgtctttccg gtcaaatcac atatacggga cataactact caattttatt
     3901 aagggagtgc aaaagtaatt attttttgtt taatgccaaa tgcttttcat aattttttac
     3961 tacagcaacc atttctttgc tttacaagac aataaactat aaatcaatta tttaacttat
     4021 taaattaagc actattctaa tggaattaat gaataatctt taatttcaat aagatttatt
     4081 ccttgatttt ctttgatgcc attggctgca tctttaacat atttaatgtt taacgtaggc
     4141 aaatagcccg ccctaagtgt gccatgtgat gtatttgatt tcacatattc cattacagct
     4201 tcatagccct gaaaagcatt caataaaggt tcggttttat agcgattaat gaaattgttt
     4261 ctgaaacttt ttgttgaagc tgcatgataa tctaaatacg ttgatgtgaa aaaagcaatg
     4321 cgcaaattct taagtatgct aaagtctatg ctttcaaaat tttcccatgt tggcagtcca
     4381 atcgcaaata caggtttttc tatgcaggca ttaacctgat tgagtatggt attgacataa
     4441 gcttcggcag atgatgttac tatcagaaca gcattttcgg ttttcaatat cttacaaacg
     4501 gaatcggggt tcacggtact tgtgataaaa tgcttcaggc gttttgccgg aagatgatct
     4561 ttaaaaatct gtgcaatgtc tttatccggt ttaacatcgc gcgcaagcag cacatatttg
     4621 gaatcgggaa accgtgttgc aatttcttta gctgccacca tgcattgtgt gtaggttgac
     4681 ggtgagagca tataaacatg ttcgctattt gctttgaaat ttatgtttga tggttgtgta
     4741 agaatcaggc tttgtttatt gttggcactc agacgagcaa cggcaggatt aaacgatgaa
     4801 ggcacagagg caatcactgc ttcacacttt acaaaattat tctgccgcca taaacgccac
     4861 agggcaaaac tatcgggagg agtttcagca atgttcaatt taatattgat tcctaattgt
     4921 tttaaactgt ctgctgccag tagtgcaccc tctgcaaact gcaggttgct caaattatta
     4981 taatctggca tagcacttcc tgaagtatca tcaaacacct gcatctgttg ttccagattc
     5041 aatggcaaca tcaacccaat attaaactct ttcttctcgg tcttttcatg aaccacttca
     5101 ggttttttca caggttgttc aacaggctta ggtgcaggtg ccggtggtgg catttgcgca
     5161 gttttcctac tgctgctgca acctgccatc aacagtaatg ccaatgccca actaaaatac
     5221 tttttgctgc tactaaaacc cataatccta ttcccattca attgttgcag gtggctttga
     5281 actgatgtca taaacaaccc tatttattcc ttttacctta ttgataattt cgttagaaac
     5341 tatacttaaa aactcataag gcagatgtgc ccagtcggca gtcataccat cggtgctggt
     5401 aactgctctt atacagattg cattttcata agtacgctca tcgcccatta caccaactga
     5461 gttaacaggt aagaaaatgg ctcctgcctg ccacactttc tgatagaggt tatgtttttg
     5521 taataaagaa ataaaaacat gatctgcttc ttgcagcatc tttacttttt ctgcacttat
     5581 ttcacctaat attctgattc ctaaccccgg acctggaaaa ggatggcgat tcaataactc
     5641 ttcttttaat cccatttctt tgcccacctt ccttacttca tctttaaaaa gcgatcgcaa
     5701 aggctcaaca attttaagat tcattttctc gggaagtcct ccaacattgt gatgcgattt
     5761 aatggttgcc gaagggccat ttacagatac tgactcaata acatcaggat aaatggttcc
     5821 ttgtgccaac cactttacat cagaaattgc atgagcttct ttatcaaaaa cttcaataaa
     5881 caccctgcca atggtttttc ttttttgctc tgggtctgat atgtttttta atgcatcata
     5941 aaatacagtg gcagcatcta cacattttat attcaatcct aatggcttaa aaacttccat
     6001 aacatgctgt gcttcatttt ttcgcagcaa gccattatca ataaaaatac aatacagatt
     6061 ttttccaata gctttatgca gaagcagtgc agccacactt gaatcaacac ctcctgaaag
     6121 ccctagaata actttatcat tctttaactt atcttgcaac tgagcaactg tttcatcaac
     6181 gaatgatgat ggtgtccagt cgccacggca gccacaaata ttataaacaa aattggctag
     6241 cattttcttt ccatcagtgc tgtgataaac ttccggatga tactgaaaac cataaacggc
     6301 taaatgcttg tgtcggaaag cagcaacagc aacatcatct gtctgtgcta tgacttcaaa
     6361 gtcattacct gcactacgaa tggtgtcggc atgcgacatc cacacctgac tgttgtcgct
     6421 tacatcatta aaaatttcat catcaaaatt aattttaaga atagctcgcc catactctct
     6481 gatttttgaa gccaccactt caccctgatg ctgatgtgca agtagctgtg ctccataaca
     6541 caatcccaac acaggtgttt taccaaaaaa acttttggtg tctataagag gcgcctgact
     6601 gtcgcgaaca gaaaatgggc ttccggaaaa aataactcct ttaatggaat cactcatttc
     6661 aggcaggtgg ttataaggat gaatctcgca ataaacatta agctcacgca gccttctggc
     6721 aattagttgt gtgtattgtg agccaaaatc aatgatcagt atggtttggt gcatgggcaa
     6781 atgtaagcag aaaagcagaa acagaaatat ctgttttttt tagcgacatg gatagttaat
     6841 tatctttgcg caaatttttt tagaatgaaa ataacttttc cggacaaaaa cgtcagagaa
     6901 tatcctgatg gaaccactgc aatggacata gccaagagca tcagcgaagg gcttgcacgc
     6961 aatgtacttg cagcaaaaat taatggtcag gtatcagacc tttcaatacc tatcaacagt
     7021 gatgcaactt tggaattatt gacatgggat agtcaggatg gtaaaaatac gatgtggcat
     7081 tcatcggcac atttaatggc agaagcattg gagttttatt ttccgggaat caaattctgg
     7141 gttgggcctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnaag attgaagaca aaatgattga gcttgccaga aaaaataatg tctatatcag
     7261 aaaagaagtt ccaaagcagg aagctatagc ctatttcaca gaaaaaaatg atgaatataa
     7321 attagacctg cttcagaacc tcgaagacgg taacattaca ttctacactc agggcaattt
     7381 tactgaccta tgtcgtggcc cacatattcc acataccggt tatattaaag cagttaagtt
     7441 gatgaacctt gccggtgcct actggaaggg tgatgaaaaa aacaaacagc ttacccgtgt
     7501 ttacggaatt acttttccga agcagaaaga gcttgacgaa tatctggcaa tgctggaaga
     7561 agccaaaaaa cgcgatcaca gaaaattagg caaagagctt gatatcttta tgtttgatga
     7621 tgaagttggc ccgggtttac ctttatggct tcctaatggt ggcgttttaa ttgagcaatt
     7681 agaaaatctg gctaagcgta ctgaaaatga agccggctac aagcgtgttc gtacacctca
     7741 tttagcaaag gaaagtatgt atttaactac agggcacctt ccttactatg cagaaagcat
     7801 gtttcctcca atggaaatgg atggagaaaa gtactacctc aagccaatga actgcccaca
     7861 ccatcataaa atatttggga atctgaatgc aagttatcgc gatttaccta ttagacttgc
     7921 cgaatatggc acctgctatc gttatgaaca atcaggagaa ttgtttgggt tgatgcgtgt
     7981 gcgttgcctt cagatgaacg atgcccatat ttactgtaca gaagaacagt ttgcatctga
     8041 gtttaaggca gttaacgaca tgtacttaaa gtattttaaa atcttcggaa tagagaaata
     8101 catcatgcgt ttttctacgc actcgccaga aaaattagga aagaaatatg ttaatgagcc
     8161 tgaactttgg ttaaaaaccg aagatatggt cagacgtgta ttgatagaat cgaacatacc
     8221 atatattgaa gtgcctgatg aaagtgcatt ttatggacct aagatagatg tacaggtttg
     8281 gagtgccatt ggtcgtgaat ttacattggc taccaatcag gttgactttg ctgttcctgc
     8341 acgttttggg cttacctatc gtgatgccga caatacaatg aaaacaccac tttgcataca
     8401 ccgtgcaccg ctgggaacac atgagcgctt tatcggattt ttgattgagc attatgcagg
     8461 taatttccct ttgtggcttg cacctatgca ggtagcaata ctgccaataa gcgaaaagtt
     8521 caatgatttt gcaaaatctg tttcacagtt actaaataat tacgatattc gcgccctcgt
     8581 tgatgaaagg ggtgagaaaa taggcagaaa aatcagagat gcggaagtta aaaaagtacc
     8641 gtttatgctg attgtcgggg agaaagagat gaatgaaaaa ctggtatctg tgcgtaagca
     8701 tggcgaagga gataaaggaa gcatgacacc tgaggcattt tgtcaattag taaagaaaga
     8761 aattgaagat caattattaa taaacaagta aaaaggaggc tatatagcaa catttaacaa
     8821 accaaatacg cccaaaccag gtggtcgttt tcctgacaga agattaccac gtcctaaagt
     8881 tgaagagcac cgcattaatg gtcgcattca ttcaccacag gttagattgg ttggcgataa
     8941 cgttgaaccc agaatttatg atacacaaac tgcactggat atggcgcagg atatgggtct
     9001 cgatttggtg gaaatatcaa atacaagtaa ccctccggtt tgtaaaatag ttgattatca
     9061 aaaattcctt tacgagagaa aaaagaaaga caaagagcaa aaagcaaagg cagctaaatc
     9121 tgttgtaaag gaaatacgct tcgggccgaa taccgatgaa catgatttta atttcaaatt
     9181 aaaacatgcc atcaaattcc tcgaagaagg agcaaaagtt aaagcttatg ttcactttaa
     9241 aggacgaagc atagcttata aagaaaaggg agaaattatc ctcctgaagt ttgcacagga
     9301 attggagcaa tatgcaaagg ttgaaatgct tccgaaatta gaaggcaacc gcatgtttct
     9361 gcacctggca ccacttgcaa agaagaagta aataaaataa taaacaaata aaaattagaa
     9421 gcgttatgcc gaaaatgaaa agaaactcca gtgccaaaaa aagattctct gttacaggca
     9481 caggtaaaat aaaaagaaag catgctttca aaagccatat cctgactaag aagtcaacga
     9541 agcgtaagcg tgcattaacc aatgctgcca tcgtacacaa gtctgacgag tttaacgtaa
     9601 aaagaatgct ttcaatagca taaaaaataa tattcaatta agtttaaccg ggtaattagc
     9661 ataaagattc cgcaaagcgg agcgctaacg ccaaaaattc aaaacacatg ccacgttcgg
     9721 taaattcagt agcatcaaga gcaagacgca aaaaactgct caaccgcgcc aaaggaaatt
     9781 ggggcgcaag aggtaacgta ttaaccgttg ccaaacacac agtagaaaaa ggtttgcaat
     9841 atgcataccg cgatcgtaaa accaagaaac gcaacttccg cggcttatgg attcagcgta
     9901 ttaatgcaga ggtaagaaat tacgatatgt cttacagcga atttatgggt aaactgcata
     9961 aagcaggtaa ccaacttaac cgcaaagttc ttgccgacct tgcacttaat aatcctgatg
    10021 cttttaaagc aattgttgag tctgtaaaat aaattgacaa tacatttttg taaaagctgc
    10081 ctcgaaaggg cagctttttt ttgtgggtaa aatttaattt acacaactta taaagtatta
    10141 acaagaatgg tgattttgtt agcattcaaa ttgaaaaatt cttcagttta aattatgttc
    10201 ttatttcgtc tatcatcaac attgttataa gttgtatcaa aataataaaa acaaaaaagc
    10261 tgttgcataa cgtgcaacag cctttaaaat aattcaaacg cttactttat ttttttgcag
    10321 caggcttatt tgatttgtaa cgcatgtcag gtgtgccatc tgcttttttt ggcccttgtg
    10381 cttttacatt tttgttctct ttaaagcgct tgtcgggggt accatccttt tttgtgtgat
    10441 ctgcagcagt actcttttct gattttactg aagtagcttt tgcagcatga gttgttgttg
    10501 ctttgttatt ggtagtagca acagtttgtg cctgggcaaa ggttaaacct aatcccaatg
    10561 caaatactaa taataacttt ttcatttttt ttgaaattta tgcaacaaat atgaaccggc
    10621 acagatgacc accacatgaa tttattttta aaacgcatgt attttttctg aagtgatatt
    10681 aatttccgac tatcactctt gcataaaaaa ataacaccat tgttagacta taacagtttt
    10741 taattaaata gacaatgctt taatgtgtaa atca
//
LOCUS       DAORQT010000035        10497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_783_length_10497_cov_18.213178, whole genome shotgun sequence.
ACCESSION   DAORQT010000035 DAORQT010000000
VERSION     DAORQT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_10497_cov_18.213178"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..580
                     /locus_tag="PK134_02440"
     CDS             35..580
                     /locus_tag="PK134_02440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRB37744.1"
                     /translation="MMLTNRSKVVVNKEMTFAEKIYIPAIVAGLMITIKHFFKKKATI
                     NFPEQQKPISEVWRGLHVLKRDEQGRENCTACGLCAVACPAEAITMTAAERKKGEEHL
                     YREEKYAAVYEINMLRCIFCGLCEEACPKEAIFLTNRMTPSDTLRDNFIYGKDKLVEG
                     IAPEERVDISYRARAHRKEVE"
     gene            637..900
                     /locus_tag="PK134_02445"
     CDS             637..900
                     /locus_tag="PK134_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37745.1"
                     /translation="MSCDKDVCRDCYLVRSMLCTYTDTTLAAYIIPGDTVKTEQQCDK
                     EKDFKDDKFKEEKYEYDSPSMFYPNYNVHVVVTDIYHCPEKTN"
     gene            1006..1461
                     /locus_tag="PK134_02450"
     CDS             1006..1461
                     /locus_tag="PK134_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRB37746.1"
                     /translation="MSALFTVFSKKPVYSVLYLIFFFFTLFGHYILLNAQFLAAVHLI
                     VYAGAIMVLFLFVIMMLNLNKEIEPNKNIVSKFAAVISSGLLLIVIVSVLKHVDVESS
                     KLNSQTDIGLVESIGHVLYNEYMLPFEMVSILLLTALIGAVMLGKKEVK"
     gene            1463..1771
                     /gene="nuoK"
                     /locus_tag="PK134_02455"
     CDS             1463..1771
                     /gene="nuoK"
                     /locus_tag="PK134_02455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRB37747.1"
                     /translation="MEPVNINHYLLLSAILFCIGVAGVLFRRNAIIIFMCIELMLNSV
                     NLLLTAFSNQHNDASGQVFVIFIMAVAAAEVAVGLAILVMIYRNVKSTDIDVLNKLKW
                     "
     gene            1775..3676
                     /gene="nuoL"
                     /locus_tag="PK134_02460"
     CDS             1775..3676
                     /gene="nuoL"
                     /locus_tag="PK134_02460"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRB37748.1"
                     /translation="MDFLISAIPLVPLIGFLLNGIINKKMPQSLVGIIASGTVLFSFL
                     ISCYLFLQINNGSIKESYYSMFSWINAGSLNLGLSFLVDRLSVIMLLVVTGVGFLIHV
                     YSIGYMKHDEDYSRFMSYLNLFIFFMLLLVMGSNYVVMFAGWEGVGLCSYLLIGFWFK
                     NNDYNKAANKAFIMNRIGDLGFLLGLFLMYQTFSSLEFKDVFAAAGNYPSGDATLTAI
                     ALFLFIGAVGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGVYMVIRSNVIYTLA
                     PFAGEIVAVIGLVTALMAASIGLKQNDIKKVLAYSTVSQLGYMFVALGVGAYTTALFH
                     VVTHAFFKALLFLCAGSVIHAMSGEQDIRRMGGLKKYLPITHFTFLMGVLAISGVPLF
                     SGFFSKDEILAVTWTYSPVMFTILAITSVLTATYMFRLYWLTFYGDFRGTHHQKEHLH
                     ESPSSMTVPLIILAVFSVIGGYIGMPEYINEKLGTHHVFTQFLNPIIYSPKEFILSHT
                     FEFTLLIGTIIGLAIIYVMTKNMYVRDKVVPEDDSQTKSFQKVLANKYYVDELYDMII
                     RKPLEAFGELFYKIFDISIIDGIVNFIPRATNLTSSGLRLLQNGSVGFYLVSMVIGII
                     LIFVFKMLL"
     gene            3677..5134
                     /locus_tag="PK134_02465"
     CDS             3677..5134
                     /locus_tag="PK134_02465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRB37749.1"
                     /translation="MNNLLLIILFLPLAGMALSLLPSREATGKTAFVFSLISLLLSVC
                     LFIQFDPNGGMQYVINYAWVPDLGINFKIGVDGISLMMILLTNVLTPLIILSSFGRRI
                     ENPKGLYALIQLMQFALVGVFISLDAFLFYIFWELALLPIYFICLLWGGKDRIRITLK
                     FFIYTITGSLLMLVGLIYLYLQTPGSHTFDIQAFYDLTLSSQQQTLVFWLIFVAFAIK
                     IPVFPLHSWQPDTYTDAPTQGTMLLSGIMLKMGLYGVLRWLLPIVPGASADNAFIVML
                     LGIIGVVYASCIAIVQKDFKRMIAYASIAHVGLITAGLFTLKVEALEGAVFQMLSHGI
                     NAVGLFFIADMLMSRLKTHNMSEMGGIANNNSLFAVLFMIVLLGTVALPLTNGFVGEF
                     LLLTGIYQFKAWMAIFGGLTIILGAVYMFRSYQAIMLGSRHLNEGEFHPMTLSEKTVL
                     GTIAFLVILLGVYPSFLMNAAAPSIKSLLMIINAG"
     gene            5160..6536
                     /locus_tag="PK134_02470"
     CDS             5160..6536
                     /locus_tag="PK134_02470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRB37750.1"
                     /translation="MNTIVTLSIVGIAAMLSEVFKLKRIMYTLVIAGLVVALGFSIND
                     WNNFGSYFNQMVMVDSFSIVFSSLMIVTTLLWMIMSRSYFSSDSSMSEHFALIVFALT
                     GGVMMTTFSNMSMLFLGLEILSFALYIMAGSDKTNIKSNEAAMKYFIMGSFATGFLLF
                     GITLIYGATSSFNIQEIANYTQQNKDALPAFFKVGVLMILIGLSFKIAAAPFHFWTPD
                     VYDGAPTLVTAFMASVVKTAAFAMIYRLFVSCFISIYDFWTPVFAVLSAITMLAGNIT
                     AVYQTSLKRMLAYSSVAHAGYMLMALAAPGIASKNALLFYLAAYSLSALGAFNLLYSV
                     HKVTGNEGIKAVKGLFYKHPVSAIMLTICMLSMAGIPPVAGFFGKYYLFTAALKENLL
                     WLVLIAILSSLIGIYFYFKVIIAAFQQPETENTGYCERPSDLAVLGICAVLSLILGIA
                     PQLISGLL"
     gene            complement(6578..8464)
                     /locus_tag="PK134_02475"
     CDS             complement(6578..8464)
                     /locus_tag="PK134_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB37751.1"
                     /translation="MNIQLQKALLLLMLFFIVFTAYSQDERDSISTHTVKEIIIQGNR
                     LETPFSEAARDIQIITQKEIAALPVQTLTELLVYVSGVDMRQRAPFGTQGDISIDGGS
                     FEQTLVLLNGIKLINAQTAHNVLNLPISLDAIDHIEVLRGAAARVYGINALTGAVNIV
                     TKKSNESFVTANIYGGTSFKRKETGDGDGMYGGGGVDLVGNFANNNHRQLLGLSQNLF
                     NGYRYNSAHKNTKIFYDGDFYINEKNSISTFAGHTYNKFGANGFYAAPGDKNSEEIVE
                     TSIFSLSSKHQLGKVNLMPRISDRYDEDDYRYFKSDLSRGRSKHFTNALMLELNGNLT
                     TSAGVIGMGWESRLTKINSSNIGEHKMDNHGAFIEFRTKLFKRLVTNVGIYVNYNSQF
                     DWKAYPGLDLAYRLNNKWKIAASFGSGQRIPSFTDLYLKQPPANVGNPDVQPETAWQY
                     DANIEFTSGHLKVNVGSFYRKVTDFIDWVRPNMTTPYSPSNFGNIIVQGIYSRINQQL
                     ILSGKHQLNFCISYNFLNPEHYDYKNEQSKYVLESLRHQLIAGVNYRYGSFNVQIENR
                     YIKRELNDAYNIVDGRISYEIHSVLFYANVSNMFDAKYKEAGAVPMPGKWFALGLKYN
                     WKKK"
     gene            complement(8759..9757)
                     /locus_tag="PK134_02480"
     CDS             complement(8759..9757)
                     /locus_tag="PK134_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37752.1"
                     /translation="MNKTFTLITTLFCAAAINTNAQLITISATHQMPAIGDTIRYVDA
                     NTFGFNQNGTGPVTNTLWDNALLMNAGTNYSFFYDDPATITGNGVDSFPGATLARGES
                     GAAGYFYYQNTANDINRIGWYSSASNYGVYNTGTYATEFHFPMTAGQTVNSTYSGRYA
                     PFGLGEDSCTLESGSLAINADQQGTLILPTGTFTNVLRIHAIETFHIKIYMLGTPVID
                     YLVSDDYYYWFHDTIKQPLLTYGITTIDGTAQTPVLRYQPVSGTTGLNQLAAHTEMTI
                     TPNPSSGKFLLSNVGSTNATIEVLNALGQKVNFQQTNMGQSLEVDLNNELEGIYFI"
     gene            9946..>10497
                     /locus_tag="PK134_02485"
     CDS             9946..>10497
                     /locus_tag="PK134_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRB37753.1"
                     /translation="MTEIMSPAGSFESLQAAIQGGADSVYFGIEQLNMRARAANNFTL
                     SDLAEIKKICSEKNVKTYLTLNTVMYDHDIMLMKKIIDAAKKNNIDAVIASDHAALMH
                     AKKIGMPIHISTQANVSNIESVRFFADYADTIVLARELTLNQVADVVKQIKRQKIKGP
                     SGKLIKIEVFAHGALCMAVSGKCY"
BASE COUNT         3152 a         1853 c         1935 g         3557 t
ORIGIN      
        1 tcattacatt tgtaaacggt tattattata attaatcatg ttaaccaata gaagtaaagt
       61 agtagtcaac aaagaaatga catttgcaga gaagatatac attcctgcaa ttgtagcagg
      121 actgatgatt actattaaac attttttcaa gaaaaaagca actattaatt ttcctgagca
      181 acaaaagcca ataagtgaag tttggcgcgg cctgcatgtt ctgaaacgcg atgagcaagg
      241 tagagaaaac tgtactgcct gcggattgtg tgcagtagcc tgccctgctg aagccatcac
      301 tatgacagct gctgaacgca aaaaaggaga agaacatttg tacagagaag aaaaatatgc
      361 tgctgtttat gaaattaata tgctgcgctg tattttctgt ggattatgcg aagaagcctg
      421 cccgaaagaa gcaatatttt taaccaaccg catgacacca tcggatactt tgcgcgataa
      481 ttttatttac ggtaaagaca aattggttga aggtattgca ccggaagaac gagtagatat
      541 aagctacaga gcaagagcac atagaaaaga agtagaatga tgggttcttt gaaaaaactt
      601 ttttccggag ttattatcac tttatcactc ggcttaatgt cttgcgataa agatgtgtgc
      661 agggattgct atttggtgcg ttcgatgttg tgtacctata ccgacactac gcttgcagca
      721 tatattatac ccggtgacac cgtaaaaact gagcagcaat gcgataaaga aaaagatttt
      781 aaagacgata aattcaagga agaaaaatac gaatatgatt caccaagcat gttttatccc
      841 aattacaatg tacatgtagt tgtgactgac atatatcatt gcccggaaaa aacaaattaa
      901 tcaatagcaa ataatcaatt aaaacaataa tttcgcacca gtttttcatt ctataatact
      961 aacgtgagcc aatacgcatt ttacatactt tcagcacttt cggtaatgag tgccttgttt
     1021 accgtatttt ctaaaaagcc ggtttacagt gtattgtatc tgatattctt cttctttaca
     1081 ttattcggtc attatattct gcttaatgca caatttctgg ctgcagtaca tttaatagtt
     1141 tatgcaggtg ctatcatggt tttgtttttg tttgtgatta tgatgctaaa ccttaataaa
     1201 gaaattgaac ccaataaaaa tattgtttca aaatttgcag ccgtcatatc cagtggtctg
     1261 ttgctgattg taattgtaag tgtactcaag catgtggatg tagaatcttc caaattgaac
     1321 tcacaaacag atataggttt ggtagaatcc attggacatg tactatacaa tgaatatatg
     1381 ctgccatttg aaatggtttc aatattactg ctcactgcat taattggtgc tgtgatgctt
     1441 ggaaaaaaag aagttaaata aaatggaacc ggttaatata aatcactacc tgctgctctc
     1501 tgccatactt ttttgtattg gcgttgccgg agtattattc agacgcaatg ccataattat
     1561 ttttatgtgt attgaactaa tgcttaactc tgtgaatctc ctattgaccg ctttcagcaa
     1621 tcagcataat gatgcatcgg gtcaggtgtt tgtgattttt attatggccg tagccgctgc
     1681 cgaggttgca gtaggattag ctattctggt tatgatttat cgtaatgtta agtcaacaga
     1741 tatagatgtt ttaaataaac ttaaatggta ggtaatggat tttttgatat cagcaattcc
     1801 tcttgttccg ctcatcggtt ttctgcttaa cggcataatc aataaaaaaa tgccgcaaag
     1861 tcttgtaggc attattgcaa gcggcactgt tctattttct tttttaattt cctgctatct
     1921 ctttttgcaa atcaacaatg gctcgattaa ggaatcgtat tattccatgt tttcgtggat
     1981 caatgcaggt agcctcaatc tgggtctttc atttttagtt gatcgcttat cggttataat
     2041 gcttttagtt gttaccggtg taggctttct gattcatgtt tattctattg gctatatgaa
     2101 acatgatgaa gattactcaa ggttcatgtc atatctgaat ctgtttatct ttttcatgct
     2161 gctgcttgta atgggaagta actatgtagt aatgtttgcc ggatgggaag gtgttggtct
     2221 ttgctcctat ttactgatag gtttctggtt taaaaacaac gactataata aagcagcaaa
     2281 caaagcattc atcatgaatc gtattggtga tttaggattt ttacttggct tgtttttaat
     2341 gtatcagaca ttttcatcat tagaatttaa agatgttttt gctgctgcag gaaactatcc
     2401 ttcaggtgat gctacgctga ctgcaattgc attgtttctt tttattggtg cagtaggaaa
     2461 aagtgcacaa ataccactct atacctggtt gcccgatgcc atggcaggac ctacacctgt
     2521 atcagctttg atacatgctg ctaccatggt aactgccggt gtttatatgg ttattcgcag
     2581 caatgtaatt tatacgcttg caccatttgc aggagagatt gtggctgtaa taggattagt
     2641 aacagcattg atggcagcat caataggtct taaacaaaat gacattaaaa aagtacttgc
     2701 ctactctacc gtaagtcagt tgggctatat gtttgtagca ttaggtgttg gtgcttatac
     2761 cactgctctg tttcacgtag taacacatgc attttttaaa gcattgcttt tcttatgtgc
     2821 cggtagtgtt attcatgcca tgagtggcga acaggacatc agacgcatgg ggggtttgaa
     2881 aaaatattta ccgataacgc attttacttt cctgatggga gttttggcaa ttagtggtgt
     2941 tccgttgttt agcggattct tttctaaaga cgaaattctt gcagtaacct ggacttattc
     3001 accggtgatg tttaccatat tagcaatcac atcggtacta actgcaacct atatgttccg
     3061 tttatactgg ttgacttttt atggtgattt tcgtggcaca catcatcaga aagagcatct
     3121 tcacgaatca ccttcgagca tgactgtacc attaattatt cttgctgttt tctctgtaat
     3181 aggaggttat atcggcatgc cggaatatat caatgaaaaa ttaggaacgc accacgtgtt
     3241 tacgcagttc cttaatccaa ttatttattc tccaaaagaa tttattttat cgcatacatt
     3301 tgagtttact ttgttgatag gcactataat tggattggcc ataatttatg ttatgactaa
     3361 aaatatgtat gtcagagata aagtggtgcc tgaagatgac agtcaaacaa aatcattcca
     3421 aaaagtactt gccaacaaat attatgttga tgaattatac gatatgataa tcagaaaacc
     3481 gttagaagcg tttggcgaat tgttttataa aatatttgac attagtatca ttgatggaat
     3541 tgtaaatttt atacctcgag ccacaaacct gacttcttcc gggttaagat tattacaaaa
     3601 cggaagtgtt ggattctatt tggtgagtat ggtcatagga atcattttga tttttgtatt
     3661 taaaatgctg ctgtagatga ataatttatt actgataatt ttatttttac cacttgccgg
     3721 catggcattg tcgctgttgc cttcacgtga agcgacagga aaaacagcat ttgtattttc
     3781 tctcatcagt ttattgcttt cagtttgctt gtttattcag tttgatccta atggtggaat
     3841 gcagtatgta ataaattatg catgggtgcc ggatttagga ataaatttca aaataggtgt
     3901 tgatggaatt tcgttgatga tgattttatt gaccaacgta ctaacaccat tgattattct
     3961 ttcatctttt ggacgcagaa tagaaaatcc taaaggactt tatgcactca ttcaactgat
     4021 gcagtttgct ttggttggtg tatttatttc gctcgatgca tttctatttt acatcttctg
     4081 ggaacttgca ctattaccta tttatttcat ttgcctgctg tggggtggca aggacagaat
     4141 acgaatcact ttaaaatttt tcatctacac catcaccgga agtttattaa tgcttgtggg
     4201 tttaatttat ctctatctgc aaacacctgg aagtcacaca ttcgatattc aggcatttta
     4261 tgatttaacg ctttcatcac aacaacagac gttagttttc tggttgattt ttgttgcttt
     4321 tgcaataaag attcctgtat ttccattaca tagctggcaa cccgacacct atactgatgc
     4381 accaacacaa ggtacgatgt tactttccgg tatcatgcta aaaatgggtc tttatggtgt
     4441 attaagatgg ttactgccta ttgttcccgg agcatcagca gataacgctt tcatcgtaat
     4501 gttacttgga atcatcggtg tggtttatgc ttcatgtatt gccatagttc aaaaagattt
     4561 taaacggatg attgcctatg catctattgc acacgtaggc ctaatcactg ccggattatt
     4621 cactttaaaa gttgaagcac ttgaaggtgc agtatttcaa atgctcagtc acggtattaa
     4681 tgctgttgga ttattcttta tagctgatat gttgatgagc agattaaaaa ctcataacat
     4741 gagtgaaatg ggtggcattg caaataacaa ttctctgttt gctgttctgt ttatgatagt
     4801 attattaggc acggtagcac ttccgttaac taatggtttt gttggagagt tcctgttgct
     4861 tacaggaatt tatcagttta aagcgtggat ggcaatattt ggcggattga caatcatact
     4921 cggtgcagta tatatgttca gaagctatca ggctatcatg cttggcagcc gccatctgaa
     4981 tgaaggagag tttcatccaa tgacattgag tgaaaaaaca gttttaggaa ctatagcatt
     5041 cttggttatt ttattaggtg tgtatccatc ttttctgatg aatgcagcag caccaagtat
     5101 aaaatcgctg ttaatgatta ttaatgcagg ataatgttta aaaataaatt aatctaattg
     5161 tgaataccat cgttacactt tctatagtcg gcattgcagc catgctttca gaagttttta
     5221 aactgaagcg cataatgtac acgctggtga ttgccggttt agttgttgca ttaggttttt
     5281 caataaacga ctggaataat ttcggttcgt acttcaatca gatggtaatg gttgacagtt
     5341 tttctatagt tttttcatca ctcatgattg taacaacctt gttgtggatg attatgtcgc
     5401 gtagttactt tagtagtgat agtagcatga gcgagcattt tgcactcatc gtttttgcac
     5461 tcaccggtgg tgtgatgatg acaacttttt caaacatgtc catgcttttt cttggattgg
     5521 agatattgtc gtttgcactt tacatcatgg ccggaagtga caaaaccaat atcaagagta
     5581 acgaagctgc catgaaatat tttatcatgg gttcgtttgc aacaggcttt ctactttttg
     5641 gaattacact gatttatgga gcaacatcat ctttcaacat tcaggaaatt gcaaactata
     5701 cacaacaaaa taaagatgca ctacctgctt tttttaaagt tggtgtgttg atgatattaa
     5761 ttggtctgtc atttaaaatt gctgctgcac cttttcattt ctggacacct gatgtgtatg
     5821 atggtgcgcc aacattagtg acagcgttta tggcatctgt agtaaagact gcagcctttg
     5881 ctatgattta caggttattt gtttcatgct tcatatctat ttatgatttc tggacaccgg
     5941 tatttgctgt tctgtcagcc ataaccatgc tggccggaaa tatcacagcg gtttatcaaa
     6001 caagcctgaa acgaatgctt gcttattcca gtgttgcaca tgcaggctac atgctcatgg
     6061 cattggcagc accgggcatt gcttcaaaaa atgcattgtt gttttatctg gcagcctatt
     6121 cactttctgc attaggtgca tttaatcttc tttactcagt acataaagtt acaggaaacg
     6181 aaggaataaa agctgtgaaa ggtttgtttt ataaacatcc tgtatcagca attatgctca
     6241 ctatttgtat gctgagtatg gcaggcattc ctcctgttgc aggatttttt ggaaaatatt
     6301 atttgtttac ggcagcacta aaagaaaatc ttctgtggtt ggttctcatt gcaatattaa
     6361 gttcgcttat cggaatttat ttttacttta aagttatcat tgcagcattt caacaacctg
     6421 aaacagaaaa cactggctac tgcgaaagac catccgatct tgccgtattg ggcatttgcg
     6481 cagtgctttc attaattcta ggtatagctc ctcaattaat ttccggattg ctctgataat
     6541 acattttacc taccgagtaa aagctttcag ataaacttta cttttttttc caattgtact
     6601 tcaggccaag tgcaaaccat tttccaggca ttggaacagc acctgcttct ttatactttg
     6661 catcaaacat attagagaca tttgcataaa ataaaacaga atgaatttca taacttatgc
     6721 gtccgtcaac aatattataa gcatcgttta gctcccgctt aatatatctg ttttcaattt
     6781 gaacattaaa actaccgtaa cgataattaa caccggcaat caattgatgt cttaatgatt
     6841 ccagcacata tttagactgt tcgtttttat aatcataatg ttcagggttt aaaaaattat
     6901 aactgatgca aaaattcaat tgatgttttc cgcttagaat caactgttga ttaattcttg
     6961 aataaatacc ttgtacaatg atattgccaa aattacttgg tgaatacggt gttgtcatgt
     7021 ttggtcgtac ccaatcaata aagtctgtaa ccttacgata gaaagaccct acatttactt
     7081 tcaaatgccc ggaagtaaat tcaatgttcg catcatactg ccatgcagtt tccggttgca
     7141 catcagggtt gcccacattt gccggaggtt gttttaaata caagtctgta aaagatggaa
     7201 ttcgctgacc tgagccaaaa cttgctgcaa ttttccattt gttatttaaa cgataagcta
     7261 aatccaatcc gggataggct ttccaatcaa actgtgaatt ataatttacg tatatgccga
     7321 cattagttac taatctctta aaaagtttgg ttctgaattc aataaaagct ccatgattgt
     7381 ccattttatg ttctccaata ttggaactgt taatctttgt caaacgcgac tcccaaccca
     7441 tacctattac acctgcagat gttgtaaggt taccgtttag ttccagcatc agtgcattgg
     7501 taaaatgttt tgaccttcct ctgcttaaat cacttttaaa atagcgataa tcatcttcat
     7561 cataacggtc acttattcga ggcattaaat tgacttttcc aagctgatgt ttagaggata
     7621 aactaaaaat tgatgtttct acaatctctt ctgaattctt atcaccagga gcagcataaa
     7681 aaccattagc tccaaatttg ttataggtat gtccggcaaa agtgcttatg gaattttttt
     7741 catttatata aaaatcacca tcataaaaaa tctttgtgtt tttatgtgcc gaattatatc
     7801 tgtagccatt aaataaattc tgacttagac ctaaaagttg tctatgatta ttatttgcaa
     7861 agtttccaac caagtcaacc cctccacctc catacatgcc gtcaccatca ccggtttctt
     7921 ttcttttaaa agatgtacct ccgtaaatat ttgcagtaac aaatgattca ttactttttt
     7981 tagtgactat attaacagca ccggtgagtg cattgatacc atataccctt gctgcagcac
     8041 cgcgaagtac ttcaatgtgg tcaatagcat ccaacgagat tggtaagttc aatacattat
     8101 gtgctgtttg cgcattaatc agtttgatac catttagcaa aaccaaggtt tgctcaaatg
     8161 atcctccatc aatactaatg tcgccttgtg tgccaaaagg agcacgctga cgcatatcaa
     8221 cacctgatac ataaacaagc aattcggtta aagtttgaac cggcaaagct gcaatttcct
     8281 tctgtgtaat tatctgaata tctcttgctg cttcactaaa aggtgtttct aaacgattac
     8341 cttgtataat tatttcttta actgtatgtg ttgaaatgct atccctttca tcttgagaat
     8401 aagctgtgaa aacaataaaa aataacatta aaagcaacaa cgctttttgc agctgtatat
     8461 tcaaattttt caaaatatat tttataaata gatgtgttta aaaaagtttt tgtcttttaa
     8521 attgtattcc aactctcaat actaattgaa aatatctcga gtttaaataa taaatttcat
     8581 cagtgttagt tgcttctctg aaaaaaaccg ccatcatttt tagtataaca gcggtttcct
     8641 tgtataagat ttatgttgat ttcttaaatc ttaactctcc aattcaaaaa aacaataact
     8701 tactaaacta aaacccaaca acacaattat gtgtagatta agtaattccg acagttactt
     8761 aaataaaata tattccttcc aattcattat ttaaatcaac ctccaaagat tgccccatgt
     8821 ttgtttgttg aaaattaact ttctgtccga gggcattcag aacttctatt gtagcattag
     8881 tgctgccaac attacttaac aaaaatttac cggaagaagg attaggtgtg atggtcattt
     8941 ctgtatgtgc tgccaactga tttaagcctg tagttcctga aacaggttga tagcgtaaaa
     9001 caggtgtttg cgctgtaccg tcaatggttg taataccata ggtaagtaaa ggttgtttga
     9061 tggtatcatg aaaccaatag tagtaatcat cagaaaccaa ataatcaatt accggagtac
     9121 ctaacatata aatttttata tgaaatgttt caattgcatg aatacgcaac acattagtga
     9181 atgtgcctgt tggcaaaatc aaagttcctt gctgatcggc attgattgca agcgatccgc
     9241 tttcaagtgt gcaggaatct tcgcccaacc cgaatggtgc atagcgtccg gagtaagtag
     9301 aattaactgt ttggcctgct gtcatcggaa aatgaaattc tgtggcatag gtacctgtat
     9361 tataaacgcc ataattagat gcgctgctat accatccaat tcgattgata tcatttgcag
     9421 tattctgata ataaaaataa cctgctgcac cactctcacc acgtgctaat gttgcaccgg
     9481 gaaaagaatc aacgccatta cctgttattg ttgccgggtc gtcatagaaa aaactgtagt
     9541 tggtgcctgc attcataagc agtgcattat cccaaagtgt attagtgaca gggcctgtac
     9601 cgttttggtt gaatccaaat gtgttggcat caacatacct tatagtatct ccaatagcag
     9661 gcatctgatg cgttgcagag atggtaatga gctgtgcatt tgtattaatt gcagcagcac
     9721 aaaatagtgt ggtaatgagt gtaaatgttt tattcatgtt atcatttttt gtgaagcaat
     9781 attaaaaaaa taattcaatg ttgcatgaat tctttttttc ataaagaagc atttctatac
     9841 gtcaggaaca atctgctaca tacctaacta tttatgctga cacaagtcat tttatatttc
     9901 atccgtttct gccatcttgc acacctttat tttactttat aaataatgac agaaataatg
     9961 tcacctgcag gctcgttcga gtcgttgcaa gcagctatac aaggtggtgc cgattccgta
    10021 tattttggaa tagagcagct gaatatgcgt gcacgtgctg ccaataattt tacactcagt
    10081 gatcttgctg aaattaaaaa aatatgcagc gaaaaaaatg ttaaaacata ccttacacta
    10141 aacacggtaa tgtatgatca cgacattatg ctgatgaaaa aaattattga tgcagcaaag
    10201 aaaaataata tagatgctgt tatagcatct gaccatgcgg cattgatgca tgctaaaaaa
    10261 attggtatgc ctatacacat ttcaacgcag gccaatgtca gcaatattga gagtgttcgc
    10321 ttttttgcag actatgccga cacaattgtg ttagcacgtg aattaacatt gaatcaggta
    10381 gccgatgttg taaaacagat caaacgtcag aaaataaaag gtccttcagg taaattaata
    10441 aaaatagaag tgtttgccca tggtgcttta tgtatggccg tttctggcaa atgttat
//
LOCUS       DAORQT010000036        10054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_856_length_10054_cov_9.000900, whole genome shotgun sequence.
ACCESSION   DAORQT010000036 DAORQT010000000
VERSION     DAORQT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_10054_cov_9.000900"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="corA"
                     /locus_tag="PK134_02490"
     CDS             <1..718
                     /gene="corA"
                     /locus_tag="PK134_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRB37754.1"
                     /translation="HHSNDTHITIQEKYQDFFDPIRLRLRNGKGNMRTYRPGYLAYAL
                     MDAVIDHYYPALEKIGLKLDDLEDELLSNPSQDSMNRILQIKRELIVFRRAIWPERDK
                     INDILRSSFDEVPEETKLFFRDSYDHCIQILDIVESYKEVTASLMDVYLSSVSHKLNQ
                     VMKVLTIISTIFIPLTFIVGLYGMNFQLANPETGERLPMNMPELYSPYGYVGVIAVMV
                     LIVVIQLYIFHRKGWIRFRE"
     gene            715..3192
                     /gene="priA"
                     /locus_tag="PK134_02495"
     CDS             715..3192
                     /gene="priA"
                     /locus_tag="PK134_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRB37755.1"
                     /translation="MTEKSSSGFYIKVVVPLPLPNLFTYSVADALISQIEIGKRVLVQ
                     FGSQKIYAAIVLETSVAKPEDYEVKPILSVIDEIPVVTEKQVALWQWVHSYYLCFWGD
                     VMQAALPAELRLSSETKIVLNTEKETGSEYLTDDEFLVLEALHSVKELSLKEISRILD
                     RKHVFPVVKTLLQKKLVLSVENIGEDFKMPMANFIRRTAQTNDQDFLKKQFDHLEKKA
                     PRQLDVLMQLMQLEKNYNEGKVPQKVLLHKSGATAAALKQLVRKGLIEILIERATGSK
                     SFFNKDFYELSEAQSTALKEITKSFTEKPVTLLHGVTSSGKTELYIRLIDECLKNGKQ
                     ALYLLPEIALTTQIITRLEKHFGNKLMVYHSRFSDNERASVYTRMIENGFTNSEPFVV
                     IGARSSVFLPFHKLGLVIVDEEHESSYKQFDPSPRYHARDTAIVLAKLFEANVLLGSA
                     TPSIETYNNAITGKYGLVELFARHADIAPPQIQLVNIKELTFKRQMKSHFSPMLVNAI
                     KEELDKGKQIILFQNRRGFAPILQCHQCNWVPHCQHCDVALTYYKKADLLRCHYCGFT
                     QQPPSKCKSCGETDIRQYGFGTEKIEDELQIFFPEHNIARLDLDSTRGKHAYSEIISR
                     FETGETDILVGTQMVTKGLDFDNVSLVGILNADNLINFPDFRAYERSFQMLMQVSGRA
                     GRKKEQGKVIIQSYNPGHSILEFVVKNDFKSFIDFELSERKKFNYPPFSRLLEITVKG
                     RDESKTAQAVNLLTTELKKHIPSILGPVTPPISRIKNFYIRTLLIKFPRQAKLNDTRQ
                     MMLSAIDRYKINPVSKNIVLQIDVDPI"
     gene            3322..5025
                     /locus_tag="PK134_02500"
     CDS             3322..5025
                     /locus_tag="PK134_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB37756.1"
                     /translation="MNKNKIIILSTCMAVALIGIIAMQVNWILHDYHLKEQQFNQRVN
                     DALTSVAIKLEKKEAFNIFSNSFVTMNNDSIFSLFRERKMLDQEWEGLDSTTEDSIAN
                     KLSPENSIRPTPPIEPPLPPDFYNQEDYKNDFIKMEIKYSGGRKITIRHNRQEITLDD
                     SSKSNTHNAVKFLSLKADVLEKQRAKIEAKINNWNKVMQNLAFEFITDEKTIEDRIKP
                     FQLDSILKTELSNRGITTPYQFGVINVGENKIMSTNAPENKIALLKSRYKTDLFPNDL
                     LAKPILLSLNFPQGFSYVLSTMWVMLISSSLFILVMLFGFSYTIYTIFRQKKLSDIKN
                     DFINNMTHEFKTPIATISLATDAVNSPAISKNTESVHRYMNIIKEENQRMHKHVENIL
                     QLALFDRKHINLNLVEFDIHEMIQKAIEQIKLQVEVKGGKIETNLNAAQHQMLGDYDF
                     IFNSIINLLDNAIKYTPENPIISINTENIQNNIRILISDNGIGMSREVQKNIFEKFYR
                     AGTGNIHDVKGYGIGLSIVKAIALAHHGEIKVLKSIKDVGTTMEFLLPLNLRKAKRKH
                     N"
     gene            5054..5761
                     /locus_tag="PK134_02505"
     CDS             5054..5761
                     /locus_tag="PK134_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB37757.1"
                     /translation="MSILTGKTKIMIAEDDTNFGLVLKDYLSLHEFDVHLYRDGRQAL
                     EKFEKGKFNLCILDVMMPVMDGFALAENIKAKDSNVPIIFLTAKSMKVDMIRGFKIGA
                     DDYITKPFDSEILLFKIKALLNRSETIVKAVNEQVEFVIGGFKFNSRLRTIQGFGKEE
                     KLSPKEAALLALLCVYLNDILPREVALRKIWNDDNYFTARSMDVFITKIRKYLKDDQN
                     IELLNVHGNGYRLVVKD"
     gene            5891..7828
                     /locus_tag="PK134_02510"
     CDS             5891..7828
                     /locus_tag="PK134_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB37758.1"
                     /translation="MALLLLYPQFASADDLNKKINLYLRDTHSAQAADYVTSIIHHYL
                     VDDEVSDSAIMWSDTLRNLGEAKGDLELVAKSDVLTGKYYLRIANDAKALDYFYRALK
                     YYKSVDNLDGEADIYLQIGLVYYLQGQFPDALPNIKLATELYQKTSNDSSLSTTYYLT
                     GLSYLELKNYKQAEIFLQQSLKIGKLLSNNQREYECYVALARLYTNQKNFVAAEQLFD
                     KVMTKWDSIIDPYGYCMALIPYSKLLIETNKKEAAFINLNRALSIAEKFNFIRFKVDI
                     YKLLAIIYSDEKNFESANYYIIQHYQLRDSIYNSENQRAINTLKAKIEAEKKQAQLDS
                     LFQKEKADQLQKWLLALTIFMLLGLSFGLYRRYNYKQKREAELRIANGELSEALNSLR
                     QVQQQLIHNEKMASLGRMSSGIAHELRNPLNFVINFTKISSELIADIDKKIQNNHDEN
                     FEQLKNNLAMIYQHSLRADKIILNFSNHAKPKSNSRTIEDLNKVVSEFTSVAYHSFIS
                     HYPDFHCHIHYDFDQSLKGIVLNLQEITTVLLNLLNNAFDAMNEKLMVDKTYKPIMKI
                     QTRQIQNSVRLSISDNGPGIDEENLPRVYEPFFTTKTLGKGTGLGLSLSYDIIKSHNG
                     DMDVKSTPHLETEFSFTLPYN"
     gene            7857..8336
                     /locus_tag="PK134_02515"
     CDS             7857..8336
                     /locus_tag="PK134_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37759.1"
                     /translation="MKVSGYILLASLAINAVFAFNKFFVSNAVANNTSVGIDAPPCAC
                     DDKERYDKNEKDGGGEWISTNNGKAASDKFKATYKTVYKGAFFSKRALDEIFCKDNRA
                     NGIVCYFGQTGEKDSTVTIMIEGTHSETTLIRSGDQNAPVAFVATVKCPVICSAVGQ"
     gene            complement(8337..8846)
                     /locus_tag="PK134_02520"
     CDS             complement(8337..8846)
                     /locus_tag="PK134_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37760.1"
                     /translation="MKKISFIIMVCFFWSCTCSVVRDEPTENIQNGNSLKSNSDSVPE
                     YIKVDASAKINESEWKPVTGVVNVSINPDGKKNYAIMLTGDGITVALTYNGKEQTGKY
                     KTGEGFNASVLDAKSAVHNCNEGYIQFSEFDTTKKVMSGKFGITCMGNKASGNGYNIQ
                     EASFNNLKW"
     gene            complement(8896..9540)
                     /locus_tag="PK134_02525"
     CDS             complement(8896..9540)
                     /locus_tag="PK134_02525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRB37761.1"
                     /translation="MHFISEELEDYCSQKTSKGSDLLQQIDRETNAKVLNPRMLSGHL
                     QGRLLSMISKMIKPTRILEIGTYTGYSALCLAEGLAEGGKITTIEKNAELESMIQKNL
                     SQSEYSDKIELHIGNALNIIPELKEQYQLVFIDADKENYPLYFKMIWDKLSSGGFVLA
                     DNVLWSGKVIEPTITGDIDTERIKMFNENVVNMPDVECVLLPVRDGLMLVRKNK"
     gene            9615..10007
                     /locus_tag="PK134_02530"
     CDS             9615..10007
                     /locus_tag="PK134_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37762.1"
                     /translation="MFRKGIRFILSFVLIFSVLASASGVTVFKMVCSKKNKTMVSLEQ
                     FSHCSNKENKECSFKRDCCQFSKSVIDANQLSNHENKVQVSTPAIVELPQLLENTNIF
                     SVFLPKFSIQEQTIPSPPLLLLISKLSI"
BASE COUNT         3531 a         1768 c         1683 g         3072 t
ORIGIN      
        1 ccatcacagc aatgacaccc acataaccat tcaggaaaaa tatcaggact tttttgatcc
       61 aattcgacta agattgagga acggaaaagg caatatgcga acatatcgtc ccggttactt
      121 ggcctatgca ttaatggatg cagttattga ccattactat cccgcacttg aaaaaattgg
      181 acttaagtta gatgatttgg aagatgaatt actaagcaat ccttctcagg attcaatgaa
      241 cagaatactt caaataaaaa gggaactgat agtattcaga agagctattt ggcctgagag
      301 agataaaata aacgacattt tacgttcatc atttgacgaa gtgcctgaag aaacaaaatt
      361 atttttcaga gattcttacg accattgcat tcagatactt gatattgttg agagctataa
      421 agaggttact gcaagtttaa tggatgtata tctctcaagc gttagtcata aacttaatca
      481 ggtgatgaaa gtactcacta tcatttcaac aatttttatc ccattaacat ttattgttgg
      541 cctgtatggc atgaactttc aacttgcaaa tccagaaaca ggagaacgtc tgccaatgaa
      601 tatgcctgaa ttgtattccc cctatggtta tgtgggtgtc attgctgtga tggtgcttat
      661 agttgttatt caattgtaca tttttcatcg taagggttgg atacgtttca gagaatgaca
      721 gagaaaagca gcagtggatt ttatattaaa gtagtagtgc cactaccact accgaattta
      781 tttacctatt cagtagcaga tgcattaatt tctcagatag aaattgggaa aagagtattg
      841 gtacaatttg gaagtcagaa aatttatgca gctatagttt tagaaacttc tgtggcaaaa
      901 cctgaagatt acgaagtcaa gcctatcctt tcagtaattg atgaaatacc agttgttaca
      961 gagaagcagg ttgcactgtg gcaatgggtt cacagttatt atttgtgttt ttggggcgat
     1021 gtcatgcaag cagcactacc ggcagaatta cgtttgagca gtgaaacaaa aattgtactt
     1081 aataccgaaa aagaaaccgg aagtgaatat ctgactgatg atgagttttt ggtattggaa
     1141 gctttacatt ctgtaaaaga attaagcctg aaagaaatat cacgtattct tgaccgcaaa
     1201 catgtatttc ctgttgttaa aacattactt cagaaaaaac ttgtattatc cgtagaaaac
     1261 attggtgaag attttaaaat gccaatggct aactttatca ggaggacagc acaaactaat
     1321 gatcaggatt ttctgaaaaa acaatttgac catctcgaaa aaaaagcacc aagacagtta
     1381 gatgttttga tgcaattaat gcaattagaa aaaaattata atgaaggcaa ggttccacaa
     1441 aaagtgcttc tccataaatc aggagcaact gcagcagcat taaaacaatt agtaagaaaa
     1501 gggttgattg aaattcttat tgaaagagca acaggctcaa agtcattttt caataaagat
     1561 ttctatgaac ttagcgaagc acaatcaact gcattaaaag aaatcactaa atcttttaca
     1621 gaaaaacctg taacactgct gcatggagta acctcatcgg gcaaaaccga gttgtacata
     1681 agacttattg acgaatgtct taaaaatggc aagcaagcac tttaccttct tcctgaaatt
     1741 gctttgacaa cgcaaatcat tacaagactt gaaaaacatt tcggcaataa actgatggtt
     1801 taccattcac gattcagtga taatgaaagg gcatctgtct atacaagaat gatagaaaat
     1861 ggctttacaa attcagagcc atttgtagta attggagcac gttcatctgt atttctaccc
     1921 ttccataaat taggtcttgt aattgtggat gaagagcatg aatcatccta caaacaattt
     1981 gatccttcac cacgctatca tgcacgcgac acagcaattg tattagctaa actttttgag
     2041 gccaatgtac ttttaggcag cgcaacccca tcaattgaaa cttacaacaa tgccatcacc
     2101 ggaaaatatg gcttggtaga actgtttgca agacatgcag atattgcacc accacaaatt
     2161 caattggtga atattaaaga actcactttt aaacggcaga tgaaatcaca tttttcacca
     2221 atgttggtta atgccataaa agaagaatta gataaaggaa aacaaatcat tctatttcaa
     2281 aacagacgtg gctttgctcc tattctgcaa tgtcatcaat gcaattgggt tccccattgt
     2341 cagcattgtg atgtagcttt aacctattat aaaaaagccg atctgttacg ttgtcattat
     2401 tgtggtttca cacaacaacc gccatctaaa tgtaagtctt gtggagaaac agatatccgt
     2461 cagtacggat ttggcactga gaaaattgaa gatgagttgc agatattttt tccggaacat
     2521 aacattgcac gtttagacct tgacagcaca agaggtaagc atgcctacag tgaaatcatc
     2581 agtcgctttg aaaccggtga gacagatatt ttagtaggaa cacaaatggt tacaaaggga
     2641 ttagacttcg ataatgttag tttggttggt atcttaaatg cggacaacct tataaacttt
     2701 ccagatttca gagcgtatga gcggagtttt caaatgttga tgcaggtaag tggtcgtgcc
     2761 ggaagaaaaa aagagcaagg aaaagtaatc attcaatctt ataacccagg gcattctata
     2821 ctcgaatttg tagttaaaaa cgacttcaaa tctttcattg attttgagtt atctgaacgc
     2881 aagaaattca actacccacc gtttagcaga cttttagaaa ttaccgttaa gggtcgtgat
     2941 gaatcaaaaa cagcacaggc cgtaaacctt ttgactacag aacttaaaaa acatatacca
     3001 tctatacttg gccctgtcac acctcctatt tcaagaataa aaaactttta tatcagaact
     3061 ttactgataa aatttccgcg tcaggctaaa ttaaatgaca caaggcagat gatgctttcc
     3121 gcaatagatc gttataagat aaacccggtg tctaaaaata ttgttttgca aatagatgta
     3181 gatcctattt aaaatttatt cttttactga aaaactaaat ctcaaaacac aggaaccact
     3241 caatagccag aattagttaa ggtgggttaa attaagttaa acaactttaa gctgtgttaa
     3301 agaattttaa atttgcatgt aatgaataag aacaaaatca tcattctctc tacatgcatg
     3361 gctgttgctc tcattggtat tattgccatg caggtgaatt ggattttgca cgactatcat
     3421 cttaaagaac aacaattcaa tcagagggta aacgatgctt tgacttcagt agcaattaaa
     3481 ctggaaaaaa aagaagcatt taatattttc agcaatagtt ttgtaaccat gaataacgat
     3541 tcaatctttt cgcttttcag agagcgtaaa atgctggatc aagaatggga aggtctggat
     3601 tccacaacag aagactcaat tgcaaataaa ctttcaccgg aaaattcaat tcgacccaca
     3661 cctcctattg aaccgccact acctcccgac ttttataatc aggaagatta caaaaatgat
     3721 tttattaaaa tggaaataaa atatagtggt ggcagaaaaa tcactataag acataatcgt
     3781 caggaaataa cgttagatga ttcttcaaaa tccaatacgc ataatgctgt taaattttta
     3841 tcgcttaaag cggatgtttt agaaaaacaa cgtgcaaaga ttgaagctaa aatcaacaat
     3901 tggaataaag tgatgcaaaa tctggctttt gaatttatta cagatgaaaa aacaattgaa
     3961 gacagaatta aaccctttca gcttgattca attttaaaaa cagaactttc aaaccgggga
     4021 ataacaactc catatcaatt tggtgtcatc aatgttggtg aaaataaaat catgtcaacc
     4081 aatgcacctg aaaacaaaat tgcactcctg aaaagtcgtt ataaaacaga tttattccct
     4141 aacgacttac ttgccaaacc tattttactt tcgctaaatt ttcctcaagg tttttcgtat
     4201 gtactttcta ctatgtgggt catgcttatt agttcatcat tatttatttt agtaatgcta
     4261 tttggattca gctatacaat ctatactatt ttccgccaga aaaaactgtc tgatattaaa
     4321 aatgacttta tcaataacat gactcatgaa ttcaagacac ctattgcgac aatctcatta
     4381 gctaccgatg ctgtgaacag tcctgctatt tcaaaaaata cggaaagtgt acatcgttac
     4441 atgaatatta ttaaagaaga aaatcaaaga atgcacaaac atgtagaaaa tattctgcaa
     4501 cttgcattat ttgaccgaaa gcatatcaat cttaatctgg tagaatttga tattcacgaa
     4561 atgatacaaa aggccattga acagataaaa ctgcaggtag aagtaaaagg aggcaaaatc
     4621 gaaaccaatt taaatgcagc acaacatcaa atgctaggcg actatgattt tatttttaat
     4681 tcaataatca acctgcttga caatgccata aaatacaccc ctgaaaatcc aatcatctct
     4741 atcaatacgg aaaatattca aaataacatt cgcatactta tttctgataa tggaatcggt
     4801 atgagccgtg aggttcagaa aaatattttt gaaaaatttt atcgggccgg aaccggtaac
     4861 atacatgatg ttaaaggtta tggtatcggg ttgtcaattg taaaagctat tgcattggca
     4921 catcatggag aaattaaagt tttaaaaagt ataaaagacg taggaacaac tatggaattt
     4981 ctattaccat taaatctgcg taaagcaaaa agaaaacata attaataaat agaacacaac
     5041 aacaaaacat aacatgtcaa tactaactgg aaaaaccaaa attatgatag ctgaagatga
     5101 caccaacttc ggcttggttc ttaaagacta tctttcttta cacgaatttg atgttcacct
     5161 gtatagagac ggcaggcaag ctcttgaaaa atttgaaaaa ggcaaattca atctatgcat
     5221 tcttgatgta atgatgcctg taatggatgg ctttgcattg gccgaaaata ttaaagccaa
     5281 agacagtaat gtgccaatca ttttcttaac tgccaagtca atgaaagtgg acatgataag
     5341 aggttttaag attggtgccg atgattacat tacgaaacct ttcgactcag aaattctgtt
     5401 attcaaaatt aaagcattac tcaacagaag tgagacaatc gtaaaagcag ttaacgagca
     5461 ggtagaattt gttataggag gcttcaaatt taacagcaga ctccgaacaa ttcagggatt
     5521 tggaaaagaa gaaaaacttt ctcctaaaga agcagcttta cttgctttat tatgtgttta
     5581 tctaaatgac atcctaccgc gtgaagtagc tttacgcaag atatggaatg acgacaatta
     5641 ctttacagct cgaagtatgg acgtgtttat taccaaaatc aggaagtatc tgaaagacga
     5701 ccaaaacatt gaattattga atgttcatgg caacggatat cgtcttgtag taaaagattg
     5761 acattttgta caatttttga ttttaatgta aaataagtat ttatctttgg ggacaaccct
     5821 gccaggtatg cctaagaaga aaaaatgcat ttatgctatc aatctgagta taaaaactct
     5881 tagatttttt ttggcattat tactgcttta tccacaattt gcctcagcag acgatttaaa
     5941 taaaaaaatt aatctgtatt taagagatac acatagcgct caagctgcag actatgtaac
     6001 ttcaattatt caccattatt tagttgacga tgaggtttct gattctgcaa ttatgtggtc
     6061 tgacacgttg cgcaatcttg gtgaagctaa aggcgatctt gagttggtag ctaaaagcga
     6121 tgtgctgacc ggaaaatatt atctcagaat cgcgaacgat gctaaagcat tagattattt
     6181 ttacagagcc ctgaaatact acaaaagtgt tgacaatctt gatggtgaag ccgacatcta
     6241 tctgcaaatc ggacttgttt actatctgca aggtcagttt cctgatgcat taccaaacat
     6301 taaactagca acagaactat atcagaagac atcgaatgat agttcccttt ctactacata
     6361 ctatctcaca ggcttaagtt atcttgaatt aaaaaattat aaacaagcag aaatttttct
     6421 tcaacaatca ttaaaaatag gtaaactttt atcaaacaat cagagagagt atgaatgtta
     6481 tgtggcatta gctcgtcttt acaccaatca aaagaacttt gtagcagctg aacagttgtt
     6541 tgataaggta atgacaaaat gggattcaat tattgacccc tatggatatt gtatggcact
     6601 aatcccatat agtaaattac tgattgaaac aaataaaaaa gaagctgctt ttataaacct
     6661 gaatcgtgca ttgtcaattg ctgaaaaatt caattttata agatttaagg tagatattta
     6721 taagctactt gcaataattt attctgatga gaaaaatttt gaatctgcaa attattatat
     6781 catacaacat tatcaacttc gtgattcaat ttataattct gaaaatcaac gtgcaatcaa
     6841 taccttaaaa gcaaaaattg aagcagaaaa aaaacaggca caattggata gtttatttca
     6901 aaaagaaaaa gcggatcaac tgcaaaagtg gctgctggca ttaactattt ttatgttatt
     6961 ggggctttca ttcggattgt ataggcgata taattacaaa caaaaaaggg aggcggaatt
     7021 acgaattgca aatggagagt tgtcagaagc attaaatagt ttaagacagg tacagcaaca
     7081 actcatacac aatgagaaaa tggcatcgtt agggcgtatg tcaagtggca ttgcacatga
     7141 actacgcaat ccattaaatt ttgtcattaa tttcactaaa atttcgagtg aattaattgc
     7201 agatattgac aaaaaaattc aaaacaacca tgatgaaaat tttgagcagt taaagaataa
     7261 tcttgcaatg atatatcagc actctttacg tgcagataaa ataattctca acttctcaaa
     7321 tcatgcaaag ccaaaaagca atagcagaac tattgaagac cttaacaaag tagtcagtga
     7381 atttaccagt gttgcatacc attctttcat ttcacattac cctgacttcc attgtcacat
     7441 tcactacgac tttgatcagt cgcttaaagg tattgtatta aatcttcagg aaatcacaac
     7501 agtattactg aacttactaa acaatgcctt tgatgctatg aatgaaaaat taatggttga
     7561 taagacatac aaaccaataa tgaagattca aacacgtcaa atacaaaact cagtaaggct
     7621 ttccatttca gacaatggtc cgggtattga tgaagaaaat ttacctcgtg tttacgaacc
     7681 cttctttact acaaaaactc ttggtaaagg aaccggctta ggcctgagcc tatcatacga
     7741 tataattaaa tcgcataatg gagacatgga tgtaaaatca acaccacatt tggaaacaga
     7801 attctcattt acattaccat ataattaatt cataaataac tttaaaatca aaaattatga
     7861 aagtatcagg ttacattttg ctggcatcgc tggcaattaa tgcagtattt gcattcaaca
     7921 aattctttgt aagcaatgct gtcgcaaaca acacatcagt aggcatagat gcaccgcctt
     7981 gtgcatgtga tgacaaagaa cgttatgaca aaaacgaaaa agatggcggt ggagaatgga
     8041 tttcaacaaa taatgggaag gctgcatctg ataaatttaa agcaacctat aaaacagttt
     8101 acaaaggcgc ctttttcagc aaacgtgcat tggatgaaat tttttgtaaa gacaacagag
     8161 caaatggcat tgtatgttac tttggtcaaa ccggagaaaa agattcaaca gtaaccataa
     8221 tgattgaagg cacacattcc gaaaccacct taatcagaag tggtgatcaa aatgcacctg
     8281 tagcttttgt tgcgacagtg aaatgtccgg ttatctgttc tgcagttggg caatagtcac
     8341 cacttaagat tattaaacga ggcttcctga atgttatatc catttccgga ggccttgttt
     8401 cccatacatg ttattccgaa tttacctgac attacttttt ttgtggtatc aaattccgaa
     8461 aactgaatat agccttcatt acaattatgt actgcagact ttgcatcaag taccgatgca
     8521 ttaaacccct cacctgtttt atactttccc gtctgttctt ttccattata agttaatgca
     8581 actgtaattc catcaccagt cagcatgatg gcataattct tcttgccatc agggtttata
     8641 gacacattaa ccacccctgt tacaggtttc cattcagact cattaatttt tgctgatgca
     8701 tcaactttaa tatattcagg tactgaatca gaattagatt ttaaactatt accattctga
     8761 atattttcag ttggctcatc gcgaacaaca gagcatgtgc agctccagaa aaagcaaacc
     8821 ataataataa aacttatctt tttcattttg tatattttca tattgaacaa atgcgagaat
     8881 ttctaattaa actttctact tattttttct aactagcatt aatccatctc ttacaggaag
     8941 caaaacacat tcaacatcgg gcatattcac cacattttcg ttaaacattt ttattctttc
     9001 tgtatcaata tcccctgtaa ttgttggttc aattactttt ccactccaga gtacattatc
     9061 tgccaacaca aatccaccag atgatagttt atcccaaatc attttaaagt acaaaggata
     9121 attttctttg tctgcatcaa taaaaactaa ttgatactgc tcctttaact ccggaataat
     9181 atttaatgca tttccaatat gtaattcgat tttatctgaa tattcacttt ggctcaaatt
     9241 cttctgaatc atactttcaa gctcagcatt tttctcaatc gtagtaattt taccgccctc
     9301 agctaatcct tctgccaaac ataatgcaga gtatccggtg taagtaccaa tttccaaaat
     9361 tcgggttggt tttatcattt tactgatcat tgacaacaaa cgaccttgca aatgtcctga
     9421 aagcattcgg ggattaagaa ctttggcatt agtctctctg tctatttgtt gtaataaatc
     9481 actgcctttt gaggtttttt ggctacaata atcttctaat tcttcactga taaagtgcat
     9541 ggtatatttt aatgcgaaag taatatttac ctcaattcat tgccatttca gaaagttgtt
     9601 actatttttg tgtcatgttt agaaaaggca tacgcttcat tctttcattt gttttaattt
     9661 tttcggtgct ggcatcagct tcgggtgtaa ctgtatttaa aatggtttgt tccaaaaaga
     9721 acaaaactat ggtatcactt gagcaattca gccactgttc gaacaaagaa aataaagagt
     9781 gtagttttaa aagagattgc tgtcagtttt caaaatcagt gattgatgcc aaccaacttt
     9841 ctaatcatga aaataaagta caggtttcaa cacctgccat tgttgaattg cctcaactct
     9901 tagagaacac aaacatattt tctgtgttcc tgcctaagtt ttcaattcag gaacaaacaa
     9961 ttccctcccc ccccttactc ctgctgattt cgaaattatc tatttgattc aggctatttt
    10021 tatttagctt atttatcaaa taataatttt aatc
//
LOCUS       DAORQT010000037         9932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_874_length_9932_cov_9.854105, whole genome shotgun sequence.
ACCESSION   DAORQT010000037 DAORQT010000000
VERSION     DAORQT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_9932_cov_9.854105"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..2162
                     /gene="feoB"
                     /locus_tag="PK134_02535"
     CDS             39..2162
                     /gene="feoB"
                     /locus_tag="PK134_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRB37763.1"
                     /translation="MSEVVLKVALVGNPNSGKSTLFNVLTGLNQKVANFPGVTVEKHV
                     GFFSLRNAFEGVDYKVELTDLPGIYSLYPKTLDEQIPYKVLTDPQDPSFPNRIIVIAD
                     ASNLKRSLFLCSQIIDLKIPVVLVLNMMDLAVHKGLLPDVKKLEQALGIKVIPAIVRQ
                     KTGIEEIKKAVLHTIPLPESDIMPATTLAPELIQEIKQSFPVKSDYAAYLLAGNYDKV
                     DISFVGNEGKQKLDMLLQKHQFSQKTMQATETLERYKTISVLLKSCVSEPASVRKSLT
                     RTVDNLVTHRIWGYGIFLAILFVIFQFIFNVAQFPMDFIDGTFADFSQKVSELLPKGV
                     LNDLIVNGILAGLGGIIIFVPQIALLFLFITILEDTGYMARVSFIMDKLLRPFGLNGR
                     SVIPLMSGVACAVPAILATRTIQSWKERIVTIMVTPLMSCSARLPVYTLLISLVIPRY
                     YVFGFINSQGLVMMLMYLIGFVAAIFSAWLFKMFVKAKQRDFFIMELPVYRLPRWKNI
                     FFVIFDKVKIFLFDAGKIIIAISIVLWFLSSRGPGKDYAETEKLYEAKAELPETTQDE
                     LNQLQAQKLEHSYAGWTGKIIEPAIKPLGFDWKIGIALVTSFAAREVFVGTMSTIYSV
                     GSEGENLPIREKMMAEKRPDGTPLYTLAVGISLMLFYAFAMQCMSTLAVVKRETGTWK
                     WPIIQFVYMSALAWVSSFAAFQLLS"
     gene            2190..2819
                     /locus_tag="PK134_02540"
     CDS             2190..2819
                     /locus_tag="PK134_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HRB37764.1"
                     /translation="MAKVTLEFIEEALKKYVPHQSIRYCIQWIEEHQINLKITRSRNS
                     KYGDYRPPQRGFGHRISINHDLNPYAFLITFIHEVAHLNQWKRKKQIIIPHGKDWKEE
                     YRKLMIPILRENIFPPDVVAALNNYMTNPSATSCSDHDLLRALRNYDREEDKWITLEE
                     LNFGVKFKIRTGRTFIKKEKLRKNYSCIDCRSKSIYFINPITEVMPVDH"
     gene            complement(2813..4225)
                     /locus_tag="PK134_02545"
     CDS             complement(2813..4225)
                     /locus_tag="PK134_02545"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB37765.1"
                     /translation="MKPAAVFNLLLKNFPFEPTNAQKHAFAGLSEFITENTAYKCFLL
                     KGYAGTGKTTVINAMIKTLYHYDFRVIMLAPTGRAAKVINHYTGDDASTIHRYLYEIK
                     SDKNGDIKTKVKPNKFKKQIFIIDESSMISNEDDKTLFKGASLLSDLVDHVYSQADNA
                     IVFIGDTAQLPPVGLQCSPALDQNFLQNNCQLQVRQYELKEVVRQQLHSGILANAHCT
                     RLLLEREEHFPRLKTSGFDDVNQVTSAELSEALSGYFPSGDDNTVTVLCRSNKQANRY
                     NEFIRKTFYGFEEVVEKNERLMVVKNNYYWHESDNDNAFIANGDEIKLIRLKKQEEYF
                     GFRFANATIELSDEKSTRLEVKLLLDTLNSESPALTYDEYKKLFDEVRNHYRKTDGKV
                     NMRKLREDPYLNALQVKYAYAITGHKAQGGQWETVFIDPGFMTEEMYGVDYMRWLYTA
                     ITRARKKVYLINVPDRFFVQ"
     gene            4437..5450
                     /locus_tag="PK134_02550"
     CDS             4437..5450
                     /locus_tag="PK134_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB37766.1"
                     /translation="MLSIDIHTHILPEKLPDWKSKFGYGGFITLDHHKNCCARMLRDD
                     GTFFREIESNCWDAKTRMNDCDVHGVHVQALSTVPVMFSYWTKPADGLAVSEFLNDHI
                     ATIVQQYPKRFVGLGTVPLQAPDLAIKEMERCKKIGLQGLQIGSHVNDWNLNDANLFP
                     FFQACAENNMPLFVHPWEMMGQKDMQRYWLPWLVGMPAETSRAICSMIFGGVFERLPN
                     LRVAFAHGGGSFPSTFGRIKHGFDCRPDLVAIDNASSPERYIGKFYFDSLVHDENMLH
                     YLINFTGANRIALGSDYPFPLGENIPGTLIKNAKLDLETKERLLGGTALEWLGLSKEM
                     FLR"
     gene            5576..6190
                     /locus_tag="PK134_02555"
     CDS             5576..6190
                     /locus_tag="PK134_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRB37767.1"
                     /translation="MDIRERILESGLELFLAHGVRRITMDDIARHLSVSKKTIYACFD
                     DKDEIVSECCQGYLKNSRCEFEHVETESKDAIHEIFLTMEKLQAMFTTINPALFEDLQ
                     KFYPRVWSMYVQFKDKHVAEVIERNLLKGIEQKLYRPDINIKILVKLRIEEITLGMNT
                     TVFPLKNFKTQDVQVALLDHYLHGIVTVKGHKLINKYKNITDEE"
     gene            6202..7551
                     /locus_tag="PK134_02560"
     CDS             6202..7551
                     /locus_tag="PK134_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB37768.1"
                     /translation="MLNQIKFLTGLLLLISINIQAQQSDKKSYSFSLKQAIDFAFQNQ
                     TDIQNAVLDQKSAQSRANELLGIGLPQISASADITHFIDIPTTFVPAEFFGGEKGSYA
                     PVQFGQPYSSTAGLSASQLLFDGSYLVGVKASRMYADLMRKQVIQTKTEKAALISKAY
                     YNVQVNSERLQMLDANVKRLKKQLDEMQVMYNNGFIEKIDLDRISLAYNNIETEKSNA
                     DRLIVLAQNLLKFQMGLNVNDELTLTDKLEDIKVVADNNLNQNIDISNRIEMDVLNTT
                     KELLRADLRLKKSSYLPSLAAFGSYSVSASRNEFDIFENKPWFRTSIIGLKLNVPIFD
                     GFQKAARVNQARFALNKMENQIQTATQGFQLEAQTAKTTLTNALGSLANQEKNRTLAA
                     DVARITKIKYQQGVGSSLEVMDAETALKVAETNYYAAMVDAFIAKVDFEKATGTLIK"
     gene            7579..8697
                     /locus_tag="PK134_02565"
     CDS             7579..8697
                     /locus_tag="PK134_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB37769.1"
                     /translation="MKKVIIAITAIGFLTACGGGNSSKKVEELEKLRKQQSELNEKIK
                     SLEAEIALTDTSRKDKIKFVAATDMSLKPFTHYIEVQANVEGDEDVVLSAKTPGTVTS
                     VNVKAGDHVTKGQVLATLDDQIMQKAIAEVQNELNLVTTLYNRQKNLWDQQIGSEVQY
                     LEAKTRKEGAEKKLASIQEQWDMTRIKAPFSGTVDNVMIKTGQLVSPQVPAIRVVNLE
                     MLKVKGELPETYIGRVNKNDNALVYFPDLGKEITSKVYYSGNAISNLNRTFNVEVRFG
                     SKENFLRPNMLAVLKIADYNKEKAMSLPVSVVQKGLDGEYVFVAENSGGKTVAKRKTV
                     KSGMTYNGNVEIVEGLNEGDKVITTGYHDLVEGDIIKL"
     gene            8712..>9932
                     /locus_tag="PK134_02570"
     CDS             8712..>9932
                     /locus_tag="PK134_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB37770.1"
                     /translation="MKDIFKEFKPSSWAIDNRTAIYIITVIITLTGIMSYINIPKESF
                     PDISLPNIYISVVYPGTSPKDMENLVVRHIEKECKGISGVKKIKSNALQDYCSIVVEF
                     NSDVKIEDAKRKVKDAVDRARNDLPQDLPNEPDIIEVNFSDLPIMAVNLSGDYDLAKL
                     KRYADEAKDKIESLKEVKEVKLIGELEREIQINVDMLKLQSSGLTMSDIERAVKYENM
                     TVPGGGVRMADGTRRSLSVSGEFTNVDQIKNLVVNSINGAPIYLKDIAEVVDSYKEQE
                     SYARLNHKNVITLSIIKRSGENLIDASDKIVSVVNELKAEAWPKDLNVVLTGDQSEQT
                     RTQLHDLINTIIIGFVLVLIVLMFFMGTSNAFFVAMSVPLSMCVAFLVLPVIGFTINF
                     IVLFSFLLALGIVVD"
BASE COUNT         3221 a         1678 c         1976 g         3057 t
ORIGIN      
        1 gtatgggtaa gtacattata aatagagggg gaatataagt gagtgaagtt gttctaaaag
       61 ttgcattggt tggtaacccc aatagtggta aaagcacgct gtttaatgta ttgacgggtc
      121 tcaatcaaaa ggtggccaac tttcccggtg tgacagttga gaaacatgta ggcttcttta
      181 gtttacgcaa tgcatttgaa ggtgttgatt acaaagttga gctaacagat ttaccaggca
      241 tttacagctt gtaccctaaa acacttgatg agcaaattcc atacaaagta ctgaccgatc
      301 cgcaagaccc atctttcccc aacaggataa ttgttattgc tgatgcatct aatttaaaac
      361 gcagtttgtt tttatgctca cagataattg atcttaaaat tcctgttgtg ctggtgttga
      421 acatgatgga cttagctgtt cataaaggtt tgttgcccga tgtaaaaaaa ttggaacagg
      481 cattaggcat caaagttatt ccggcaattg tacgtcagaa aacaggtatt gaagaaatca
      541 agaaggctgt tctgcatact attcctttgc ccgaaagtga tattatgcct gcaacaaccc
      601 tggctcctga acttattcag gaaatcaaac aaagttttcc ggttaaaagt gattatgcag
      661 cctacctgct tgcaggtaat tatgataagg tggacataag ttttgttggt aatgaaggta
      721 aacaaaaatt ggacatgctt ttacagaagc atcagttcag ccaaaaaact atgcaagcta
      781 ctgaaacatt ggagcgttat aaaactattt ctgttcttct taaaagttgt gtcagcgagc
      841 ctgcatcagt gcgtaaatcc ctgacccgta ccgttgataa tctggttaca cacagaatat
      901 ggggctatgg aatttttctg gctattctgt ttgtaatctt tcagtttata tttaatgtgg
      961 cacagtttcc gatggatttt attgacggaa cctttgctga tttttctcaa aaagtaagcg
     1021 aattgcttcc taaaggtgtg ttgaatgatt tgattgtaaa tggtattctt gcaggtcttg
     1081 gtggcatcat catatttgtt ccgcagattg ccttattgtt tttatttata accatccttg
     1141 aagacacagg ctatatggca cgtgttagct ttattatgga taagttgctc aggccatttg
     1201 gattaaacgg aaggtctgta attccattga tgagtggtgt ggcatgtgcc gtgcctgcaa
     1261 tccttgcaac acgaaccata caaagttgga aggagcgtat tgtaacaatt atggtaacac
     1321 cattgatgag ttgttcggca cgactacctg tttataccct gctcatttca ttggttattc
     1381 cacgatatta tgtttttgga tttatcaatt ctcaggggtt ggtgatgatg ctcatgtatc
     1441 ttatcggttt tgttgcagct atattttctg catggttgtt taagatgttt gttaaagcca
     1501 agcagcgtga tttttttatc atggaattac cggtttaccg tttgccacga tggaagaata
     1561 tcttctttgt aattttcgat aaagtaaaaa tatttttatt cgatgcagga aaaattatca
     1621 ttgccatttc tattgtactt tggtttttat catctcgtgg accgggaaaa gattatgccg
     1681 aaacagaaaa actatatgaa gcaaaggcag aacttcctga aacaacacag gacgaactga
     1741 accaattaca ggcacagaag ctggaacatt cctatgcagg atggacagga aaaattattg
     1801 aacctgcaat taaacctttg ggcttcgact ggaaaattgg aattgccttg gtgacatcat
     1861 ttgcagctcg cgaagttttt gttggaacca tgtcaaccat ttacagtgta ggtagtgaag
     1921 gtgaaaattt accaatcaga gaaaagatga tggcagaaaa acgacctgat ggaacacctc
     1981 tttatacatt agctgttggc atttcactga tgttgtttta tgcatttgcc atgcaatgta
     2041 tgagtactct tgctgttgtt aaacgagaaa ccggcacatg gaaatggcca atcattcagt
     2101 ttgtgtatat gagtgcttta gcatgggtat caagttttgc tgcttttcag ctactgagtt
     2161 aatttattat ttttcgaccg aaattaatta tggcaaaagt tactctcgag tttatagaag
     2221 aagcattaaa gaaatatgtt ccgcatcaat ctatcagata ttgcatacaa tggattgaag
     2281 aacatcagat caatcttaaa attacaagaa gccggaatag caaatatggt gactatcgtc
     2341 cgccacaaag aggcttcggc catcgtattt ctatcaatca cgatcttaat ccttatgctt
     2401 ttctcatcac attcattcat gaagtagcac atctaaatca gtggaaacgc aagaaacaaa
     2461 ttattattcc acacggaaaa gactggaaag aagagtatcg taaactgatg attcccattc
     2521 tacgcgagaa tattttcccg cccgatgttg ttgctgcact gaataattat atgacaaatc
     2581 catcagctac aagttgctca gaccatgact tgcttagggc attgcgcaat tatgacagag
     2641 aagaagataa atggattaca ctggaagaac tgaattttgg tgtcaagttt aaaataagaa
     2701 caggccggac ttttattaag aaagaaaaac tacgtaaaaa ttatagttgt attgattgtc
     2761 gttctaaaag tatttacttc atcaatccca taacggaagt gatgcctgta gatcattgaa
     2821 caaagaaacg atcaggaaca ttaataagat aaactttttt gcgtgcacgt gttattgctg
     2881 tatatagcca tcgcatataa tcaacaccat acatttcttc tgtcataaag cccggatcaa
     2941 taaacactgt ttcccattgt ccgccttgtg ctttgtgacc tgtaatagca taggcatatt
     3001 ttacctgcaa tgcattgaga taaggatctt ctcgtagttt acgcatgttt actttgccat
     3061 ctgttttgcg ataatgattt ctcacttcat caaacaattt tttatactca tcataagtta
     3121 atgcaggcga ctcgctgttt aatgtatcaa gcaaaagttt tacttcaagc cttgttgatt
     3181 tttcatcaga taattctatt gtagcattgg caaaacgaaa cccaaaatac tcttcctgct
     3241 tttttaaacg aatcagttta atttcatcgc cattggcaat aaatgcattg tcattgtctg
     3301 attcatgcca gtagtaatta ttttttacaa ccatcagcct ttcgtttttc tctactacct
     3361 cttcaaagcc ataaaaagtt tttctgataa attcattgta tcggtttgcc tgcttattac
     3421 ttctgcacaa cacagttact gtgttatcat caccggaagg gaaatatccc gaaagtgctt
     3481 cagagagctc tgctgaagtt acttggttta catcatcaaa tcctgatgtt tttaggcgag
     3541 gaaaatgctc ttctctttca agcaataatc gtgtgcaatg tgcattagct aatattccgg
     3601 agtgaagttg ttgccgcacc acttctttca attcatattg ccttacctga agctgacagt
     3661 tgttttgaag aaaattctga tccaatgcag gactgcattg caaaccaacg gggggcaatt
     3721 gtgcagtgtc gccaataaat actatagcat tgtctgcctg actgtaaaca tgatctacta
     3781 agtcagaaag taaacttgct cctttaaaca atgtcttatc atcttcattc gaaatcattg
     3841 aagactcatc aataataaaa atctgctttt taaatttgtt tggctttacc tttgttttga
     3901 tgtcgccatt tttatcactt tttatttcat acaagtaccg atgaattgta gaagcatcat
     3961 caccggtata atggttaatc actttagctg ctctgcctgt cggggcaagc attatcactc
     4021 tgaaatcgta atgataaagt gtttttatca ttgcattaat taccgttgtt tttcctgttc
     4081 cggcatagcc ttttaaaaga aagcacttat atgcagtatt ttctgtaata aactccgata
     4141 gtcctgcaaa agcatgcttc tgtgcattgg taggttcaaa tggaaaattc ttcagtaaga
     4201 gattaaaaac tgcagcaggc ttcaatgttt atcttgcatt attatcggat aaaaatacaa
     4261 aaggggaagt taacatcata agatagataa ccaatgataa taacaatgat gtaaagagtt
     4321 gttattacta aacaaacatt taattagtag aataatattt tgaatcccac actctgaatt
     4381 tttaattatc acctcattaa tctttgtact ttcgtgcatt cttaaaattg taatacatgc
     4441 tcagcataga cattcacaca catatactac cggagaaatt accggattgg aagtcgaaat
     4501 ttggctatgg tggttttatc acattagatc atcacaaaaa ttgttgtgcc aggatgttgc
     4561 gtgatgacgg cacttttttt cgtgagattg aaagtaattg ctgggatgca aagacaagaa
     4621 tgaatgactg cgatgtacat ggtgttcatg ttcaggcatt gagtactgta cctgtcatgt
     4681 tttcatattg gacaaaacct gcagatggac ttgctgtatc agaattttta aatgatcata
     4741 ttgcaacaat tgttcaacaa tacccaaaac gttttgtagg cttaggtact gttcctctgc
     4801 aagcaccgga tttagctata aaggagatgg aaaggtgtaa gaaaattgga ctgcaaggct
     4861 tacaaatagg ctcgcatgta aacgactgga atcttaatga tgcaaacctg tttccgtttt
     4921 ttcaggcatg tgcagaaaac aacatgccgc tttttgttca cccatgggaa atgatgggtc
     4981 aaaaagatat gcaacgttac tggctgcctt ggttggtggg aatgccggca gaaacttctc
     5041 gtgctatttg ttcaatgatt tttggaggtg tgtttgaaag attgccaaac ttgcgtgttg
     5101 cttttgctca tggcggagga agttttccgt caacattcgg acgcattaaa catggttttg
     5161 attgccgccc cgatttggtt gcaatagata atgcatcttc accggagaga tatatcggta
     5221 agttttattt tgactcattg gtacatgatg aaaacatgtt gcattacctg attaatttca
     5281 caggagccaa tcgtattgca ttaggaagcg actatccttt tcctttaggt gaaaatattc
     5341 ccggaaccct gattaaaaat gcaaaactcg atttagaaac taaagaacga ttgcttggtg
     5401 gcacagcatt agagtggttg ggtttatcaa aagaaatgtt tctgagatag attttctact
     5461 ttcattcaat ctcccaaaac aatgttaaaa ttgttaaaga cttcttaaat tttttctcgg
     5521 aaacttaaaa aactaaaata gtttccatag ttttggtcag tttttaacaa ttatcatgga
     5581 tataagagaa agaattttag agtcggggtt ggagttgttt ctggcacatg gtgtcaggcg
     5641 tataaccatg gacgacatag caagacattt gtccgtttct aaaaaaacaa tttatgcctg
     5701 ctttgatgat aaggatgaaa ttgtatctga atgttgtcag ggatatttaa aaaacagccg
     5761 ttgcgagttt gaacatgttg aaacagaaag caaggatgcc attcacgaaa tatttctgac
     5821 aatggaaaaa ttgcaagcga tgttcaccac catcaaccca gcattgtttg aagacttaca
     5881 aaaattttat ccgcgagtgt ggagtatgta tgttcagttt aaagataaac atgttgcaga
     5941 agtcattgaa agaaatcttt taaaaggcat tgagcaaaaa ctttaccgtc cggatatcaa
     6001 tattaaaata ttggtaaaac tgagaataga agaaattact ctgggaatga atactacagt
     6061 gtttccttta aagaacttta aaacgcagga tgttcaggtt gcattgctcg atcattatct
     6121 gcatggcatt gtaaccgtta aaggacataa actcatcaat aaatataaaa acataacaga
     6181 cgaagaataa ataacaaccg tatgctgaat caaattaaat ttcttacagg tttactgttg
     6241 ctgatatcaa tcaatataca ggcgcagcaa tccgataaaa agtcttattc cttttcattg
     6301 aaacaggcaa ttgattttgc ttttcaaaat cagaccgata ttcaaaatgc agttctcgat
     6361 cagaagtcag cacaaagcag agctaatgaa ttattgggaa ttggtttacc gcaaatcagt
     6421 gccagtgccg acatcacaca ttttattgac ataccaacaa cctttgtgcc tgcagaattt
     6481 tttggtggcg agaaaggtag ctatgccccc gtacaatttg gccagccata tagctctact
     6541 gccggtttat ctgcatcaca actattgttt gacggatctt atttagttgg cgtaaaagca
     6601 tcacgcatgt atgccgattt gatgcgtaag caagttattc agactaaaac tgaaaaagct
     6661 gcactcatca gcaaggcata ttataacgtg caagttaaca gtgagcgttt gcagatgctc
     6721 gatgcaaatg ttaagcgatt aaaaaagcag cttgatgaaa tgcaggtaat gtacaataat
     6781 ggttttatcg aaaagattga tttagaccga atcagtcttg cctataacaa tattgaaacc
     6841 gaaaaatcaa atgcggacag actgattgtg cttgcacaaa acctgttgaa atttcagatg
     6901 ggattgaatg ttaatgatga gttgacactc acagataaat tggaagacat aaaagttgtt
     6961 gccgacaata acctgaatca gaacattgat ataagcaaca gaatagaaat ggatgttttg
     7021 aatacaacca aagaattact cagagctgat ttaagattaa aaaaatcttc ttaccttcct
     7081 tcacttgcag cctttggctc gtatagtgta agtgcatcgc gcaatgaatt tgatattttt
     7141 gaaaacaaac cctggtttcg tacttcaata attggcttga aacttaacgt acccattttt
     7201 gatggctttc agaaggcagc acgagttaat caggcaagat ttgcgcttaa taaaatggag
     7261 aatcaaattc agactgcaac acaaggcttt caattagaag cgcagactgc aaagacaact
     7321 ctaaccaatg cattaggctc tcttgccaat caggaaaaaa acagaacatt agctgctgat
     7381 gtagcaagaa ttacaaaaat aaaatatcag caaggtgttg gcagtagcct tgaagtaatg
     7441 gatgcggaaa cagctttgaa agtagcagaa acaaattatt atgctgcaat ggtagatgca
     7501 tttattgcca aagtagattt tgagaaagca acaggaacat taataaaata aaatataaac
     7561 tctgacataa ataaatatat gaaaaaggta attatagcaa taacagcaat tggatttctt
     7621 actgcctgtg gtggaggaaa cagcagtaaa aaggttgagg aacttgaaaa actcagaaag
     7681 caacagtctg agttaaatga aaagataaaa tctttagaag cagaaattgc actcacagat
     7741 acttccagaa aagacaaaat aaaatttgtt gcagccacag atatgagttt gaaaccattt
     7801 actcattata ttgaagtgca ggcaaatgtt gaaggtgatg aagatgttgt attgagtgca
     7861 aaaactcccg gaactgttac ctctgtaaat gtaaaagctg gcgaccatgt aaccaaaggt
     7921 caggtgttgg caacgctcga tgatcagatt atgcagaagg caattgcaga agtacagaat
     7981 gaacttaatc tggtgacaac actttataac cgtcagaaaa atctgtggga ccaacagata
     8041 ggtagtgaag tacaatatct ggaagcaaaa acaagaaaag aaggtgcaga gaaaaaactt
     8101 gcatctattc aggagcaatg ggatatgact cgtattaaag caccattcag cggaacagta
     8161 gataatgtga tgataaagac aggacagttg gtttcaccac aggttcctgc aataagagtt
     8221 gttaatctcg aaatgcttaa agtaaaaggc gagttacctg aaacttatat cggaagagta
     8281 aataaaaatg ataatgcatt ggtttatttt cctgacttag gaaaagaaat tacaagtaag
     8341 gtttattatt ccggaaatgc catcagcaac ctgaaccgta catttaatgt tgaggttcgt
     8401 tttggcagta aagaaaattt cctgcgacct aatatgcttg ccgttttaaa aatagctgat
     8461 tacaataaag agaaagcaat gtcacttcct gttagtgttg tacaaaaagg acttgatggt
     8521 gagtatgttt ttgttgctga aaattcagga ggcaaaactg ttgcaaagcg taaaacagta
     8581 aaaagcggaa tgacatataa tggcaatgtt gaaattgttg aaggattgaa tgaaggtgat
     8641 aaagtgatta caacaggtta tcacgatttg gttgaaggcg atatcattaa actataaacc
     8701 gcaagcattt tatgaaagat atatttaaag aattcaagcc ctcatcatgg gcaatagata
     8761 accgtacggc tatatacatt attaccgtca tcattacatt gacgggtata atgtcataca
     8821 tcaatattcc gaaggaaagt tttccagaca tttctttacc aaatatttat atcagtgttg
     8881 tttatccggg cacttcacca aaggatatgg agaacttagt ggtgcgccac attgaaaagg
     8941 agtgtaaagg aatttcaggt gtaaaaaaaa tcaagagtaa tgcactgcag gattattgta
     9001 gtatagtggt agaatttaac agcgatgtaa agattgaaga tgcaaagcga aaagttaaag
     9061 atgctgttga ccgtgcacgt aatgatctcc cgcaggattt acccaacgag cctgatatta
     9121 tagaagtcaa cttttctgat ttgcctatta tggctgtaaa cctttccggt gattatgatc
     9181 ttgcaaaatt aaagcgctat gccgatgaag ctaaggataa aatagaaagc cttaaagaag
     9241 ttaaagaagt taaactgatt ggagagttag agcgtgagat tcagataaat gttgacatgc
     9301 ttaagctgca atcatcagga ttaacgatga gtgatattga acgtgcagtt aagtacgaaa
     9361 atatgactgt gcccggtggc ggtgtgcgca tggccgatgg tacacgcagg tcgttgagtg
     9421 tttcgggtga gtttaccaat gttgatcaga taaaaaactt agttgtaaat tctattaatg
     9481 gtgcacctat ttatttaaaa gatattgcag aagtagttga ctcctacaaa gagcaggaaa
     9541 gctatgcacg tctaaatcat aaaaatgtaa ttacactaag cattatcaaa cgctccggtg
     9601 aaaacctgat tgatgcatca gataaaattg tttctgttgt taatgaactt aaagcagaag
     9661 cctggccaaa agatttgaat gttgtactta caggtgatca gagtgaacaa acacgtacgc
     9721 agttgcacga cctgattaat accatcatca tcggttttgt attggtatta attgtactta
     9781 tgttttttat gggaacaagc aatgcattct ttgttgccat gtctgtgcca ctatctatgt
     9841 gtgtggcatt tttggtttta cctgttatag gatttacaat taacttcatt gtattattct
     9901 cttttctgct tgcattagga attgttgttg ac
//
LOCUS       DAORQT010000038         9921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_878_length_9921_cov_12.251368, whole genome shotgun sequence.
ACCESSION   DAORQT010000038 DAORQT010000000
VERSION     DAORQT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_9921_cov_12.251368"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK134_02575"
     CDS             complement(<1..563)
                     /locus_tag="PK134_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37771.1"
                     /translation="MKKLFTLIFFAIAGLVVADILNEQGQAVATGSPGEFDCTSCHTG
                     NNVNSGTGSISISSPNMPNWQYVPGQVYQIDVTVAQTGAPLFGFGFEALRTSNNTNGG
                     TFQITNTSSTFLKTGFINGSNRTNVVHKKNGGLSNDSHTFSFNWTAPATNIGNIIFYS
                     AGNAANNQGDTLGDFIYKTQQLITPSIT"
     gene            complement(952..2568)
                     /gene="pckA"
                     /locus_tag="PK134_02580"
     CDS             complement(952..2568)
                     /gene="pckA"
                     /locus_tag="PK134_02580"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRB37772.1"
                     /translation="MNEYGLRNPNFSVAELGLKNVAAAYWNLTVAELVEEVVIRGEGE
                     LTDFGALAVDTGEFTGRSPKDKFIVADKETENSVWWGDINIKFDADKFDALYNRVTAY
                     MSGKEIYVRDCYACADPRYRLNIRVITETPYQSIFSNNLFLRPTPEEITTMEKDWVIM
                     AASGFMADPKIDGTRQHNFAIVNFTKKIILIGGTGYTGEIKKGIFSVLNYILPHHKNV
                     LSMHCSANIGKNNDTAIFFGLSGTGKTTLSADPNRGLIGDDEHGWSDNTVFNFEGGCY
                     AKCVNLSKEKEPQIYQAIRFGALLENTEFFEGTTRVDFDNISKTENTRVAYPIHFIDN
                     AVEPSVAGIPKNIFFLTCDAFGVLPPISRLTTGQAMYHFISGYTAKVAGTEVGITEPT
                     TTFSACFGKAFLPLHPTKYAELLGKKLSENKDIRVWLVNTGWSGGSYGVGSRIKLAYT
                     RAIITAALNGELDNVKFETLPVFGLQYPVSCPNVPAEILNPKATWADKTSFDNKSADL
                     AAKFVKNFEQYASHANKEIMDEAPKVAVVA"
     gene            2746..4227
                     /gene="accC"
                     /locus_tag="PK134_02585"
     CDS             2746..4227
                     /gene="accC"
                     /locus_tag="PK134_02585"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRB37773.1"
                     /translation="MFKKILIANRGEIALRVMRTASDMGIKTVAVYSDADRMAPHVLY
                     ADEAVHIGASPSAQSYLVVEKIIQAAKLTGAEAIHPGYGFLSENADAAQAIINAGLVF
                     IGPSPQSMRTMGSKLGAKEAAKKFNVPLVPGTDYAISDIDKARQIAVDIGFPVLIKAS
                     AGGGGKGMRIVESEKAFNESVKSAMSEAQSAFGDGSVFIEKYIASPRHIEIQVMGDNH
                     GNVVHLFERECSVQRRHQKVIEEAPSGVLDEKTRSAMGQCAVMVAKACDYSGAGTVEF
                     LLDEKNNFYFLEMNTRLQVEHPVTEMITGIDLVREQLNVAAGNKLSFSQDDLKIHGHA
                     VEVRVYAEDPANNFLPSVGKLTTYVPPRGVGVRVDDGYEQGMEIPIYYDPMIAKLVTY
                     GRDRNEAIARMIRAIDEYKITGCETTLPFCRFVMEHKAFVTAKFDTHFVKKYFTPVVL
                     EKTIADEAEIASLAAQLWLDEKKPEGIQQQQEKSISYWRNNRS"
     gene            4305..4895
                     /locus_tag="PK134_02590"
     CDS             4305..4895
                     /locus_tag="PK134_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRB37774.1"
                     /translation="MKFSVWLCFLLTAFIKFSIGQTPVGSSTAIYDGDTIAYYTLNAV
                     DIYGFIPANASDDVRKYMKLRRDVLKAYPYAKIASAELRFIEESVSKMTSEKEKKKFI
                     KEQEKIMKEQFEKDLKNLTYTQGKILIKLIDRETGHTSYNLVKDLRGSLQAFFWQGIA
                     KIFSANLKSEYDPDGADKMIEKVVQSIEKGELQVRN"
     gene            4958..6427
                     /gene="guaB"
                     /locus_tag="PK134_02595"
     CDS             4958..6427
                     /gene="guaB"
                     /locus_tag="PK134_02595"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRB37775.1"
                     /translation="MALASKSNLQEGLTYDDVLLVPAFSEILPRDVDTSTSFSRNIKL
                     NVPIVSAAMDTVTESAMAIAIAQEGGIGVLHKNMSIEQQTNEVRKVKRSESGMIHDPI
                     TLHQNSTIHEALGIMREQRIGGIPIVNDSKKLVGILTNRDLRFETDESRKVKEVMTSG
                     QLITVTGVVSLQKAEVILRQYKIEKLPVVDRSGKLIGLVTYKDIIKFKSRPNSCKDKL
                     GRLRVAAGIGVTADMLDRVASLVNAGVDAIVIDTAHGHSKGVITALKSVKKKFSGLDV
                     VVGNVGTAEGAKLLVKNGADGVKVGIGPGSICTTRVVAGVGVPQLTAVADAAGAIKGS
                     GVPIIADGGIRFTGDIPKAIAAGANSIMAGSMFAGVEESPGETIIFEGRKFKSYRGMG
                     SIEAMREGSKDRYFQDAEDDIKKLVPEGIVGRVPYKGTLAETVYQLVGGLRAGMGYCG
                     AKNIAELQKAKFIRITQSGVRESHPHGITIVKEAPNYTR"
     gene            6771..9389
                     /gene="clpB"
                     /locus_tag="PK134_02600"
     CDS             6771..9389
                     /gene="clpB"
                     /locus_tag="PK134_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRB37776.1"
                     /translation="MDINKLTIKSQEVLQQAQFLAKSFGQQTVETGHLLKSMLEVDEH
                     VVPFLLKKASVNINSLTQKLDNIIQSYPKVSGGQVYLSTDVNDAMQRAFAALKVNKDE
                     FVSVEHLLLALLSGKDKVAKLLKEEGCNEKDLQNAINELRKGERVTSASAEETYNSLN
                     KYARNLNELARNGKLDPVIGRDDEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIINGDVPENLKSKVIYSLDMGALIAGAKYKGEFEERLKAVVKEVISGEGEIILFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAVGATTLNEYQKYIEKDKALERRFQKV
                     MVEEPDTESAISILRGLKERYETHHKVRIKDEAIIAAVELSQRYINDRYLPDKAIDLV
                     DEASSKLRLEMDSVPEELDELNRRIMQLEIEREAIKRENDTKKVNELSEEIAQLNEQR
                     NAVKARWQSEKEVVEKIQDIKEKIENLKLEAEQAERSGDYGRVAEIRYGKVREAEETL
                     SKLMEQLNEMHSKGAMMKEEVDSEDIAEVVSRWTGIPVNRMLQSEREKLLHLEAELHK
                     RVVGQDDAIEAVADAVRRSRAGLQDMRKPIGSFIFLGSTGVGKTELAKALAAYLFNDE
                     NNMTRIDMSEYQEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVILLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTANFKNTIIIMTSNIGAHLIQENFENIDERNRDEVIA
                     KTKNNVFDLLKKTIRPEFLNRIDEVIMFNPLTRNQIEEIVRIQFNQAVDMLAKNDIKL
                     SITDRAIEWVAQLGYDPQFGARPLKRVLQKKILNELSKQILAGTINKEDVILIDLDKK
                     KNFIFENEKTHTEIKA"
     gene            complement(9424..9723)
                     /locus_tag="PK134_02605"
     CDS             complement(9424..9723)
                     /locus_tag="PK134_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRB37777.1"
                     /translation="MKKAWEKVPDFLKNKYAICIIAFVVWLAFFDKNDFYAQYRFRQQ
                     LNTLKNDKSYYADELEKSKSDLNELISSPANLEKFAREKYLMKKDDEDIFVFVEK"
BASE COUNT         3289 a         1675 c         2036 g         2921 t
ORIGIN      
        1 gtaatagaag gcgtaataag ttgttgtgtt ttgtaaataa aatcacctaa tgtatctcct
       61 tggttgtttg cagcatttcc agctgaatag aaaataatat ttccaatgtt ggttgcagga
      121 gctgtccagt taaatgaaaa tgtatgcgaa tcatttgaga gtccgccatt tttcttatgg
      181 acaacattgg ttctgtttga accatttata aaacccgtct taagaaatgt gctggaagtg
      241 ttggtaatct gaaaagtacc tccgtttgta ttgttacttg tccggagtgc ttcaaaccca
      301 aatccaaaca gcggagcacc agtttgtgca actgtaacat caatttgata cacctggcct
      361 ggaacatatt gccagttagg catatttggt gatgaaattg aaatggaacc ggtaccgcta
      421 tttacattat tgcctgtatg acatgatgta caatcaaatt caccgggaga acctgtggca
      481 acagcctgtc cttgttcatt taaaatatct gcaacaacaa gacctgcaat tgcaaagaaa
      541 attagtgtaa agagtttctt cataacctgt atttttattt taacaggtca aatatgatga
      601 caatttgtaa aaatcaaaaa agcaatagct ctaaaaggaa taatttcctt ccgaaaaggg
      661 gataaactta tctgtactgt ttaaaaacta cggcaacaaa ttacttgctg acatgaaaat
      721 ggaaatggtg gcggttgctt tttgaacgca ccttaataca gaaaaagaaa acgatggctg
      781 taatgttgag taatgtaaat agtaataaca tgcctctaaa gtaatatcaa catcgaagag
      841 aatatatact atttggtaaa tggaaagttt ttttaggcaa aaatcaattc agcataagtc
      901 aaaaaaaact cccccgcgat tttcgcgggg gagaaaaaaa attaaaaaaa attaagctac
      961 tacagccacc ttaggtgctt catccataat ttctttatta gcatgtgaag catactgctc
     1021 aaaattcttc acaaacttag cagctaaatc agctgattta ttgtcaaatg aagttttatc
     1081 tgcccatgta gctttgggat tcagaatttc agcaggcaca ttaggacatg aaacgggata
     1141 ttgtaatcca aacaccggta aggtttcgaa cttaacatta tctaattcac catttaatgc
     1201 agcagtaatg atggcgcgtg tatatgcaag tttaattctt gaaccaacac cataagaacc
     1261 accgctccat cccgtattca ctaaccaaac acgaatgtct ttattttcac ttagtttctt
     1321 accaagtaat tcagcatatt tcgttgggtg caggggtaga aatgctttac caaaacaagc
     1381 agagaatgtt gttgtaggtt cagtaatgcc aacttctgtt cctgcaactt ttgcagtata
     1441 tccactgatg aagtgataca ttgcctggcc tgttgtaagt cttgaaatag gaggcaatac
     1501 accaaaggca tcgcaagtta aaaagaagat gttctttgga ataccggcca ctgaaggttc
     1561 aacagcatta tctataaaat gtattgggta ggctacacga gtgttttctg tttttgaaat
     1621 attgtcaaaa tcaacacggg ttgtaccttc aaagaattct gtattttcta agagtgcacc
     1681 aaagcgtatt gcctgataaa tctgtggttc tttttctttg ctcaggttta cacatttagc
     1741 ataacatcca ccttcaaaat tgaatacagt attgtcgctc caaccatgtt catcatcacc
     1801 aattaaacct ctgttagggt cggcactcaa tgttgttttt cctgtaccgg aaagtccgaa
     1861 gaagatagca gtatcattgt ttttaccaat gtttgcagag cagtgcatac tcaatacatt
     1921 cttatgatgt ggcagaatgt aattcagtac agagaaaatt ccttttttta tctctcctgt
     1981 ataacctgta ccgccaataa gaataatctt tttggtaaaa ttaacgatgg caaaattatg
     2041 ctgacgcgtt ccatcaattt ttggatcggc catgaaacct gatgctgcca tgattaccca
     2101 atctttttcc atagtagtaa tctcttccgg tgtaggtctt aaaaataagt tgttagagaa
     2161 aatactttga tatggcgttt cggtaataac acgaatgttt aaacggtagc gtgggtctgc
     2221 acaagcatag cagtcacgta cataaatttc tttgccggac atatatgctg taactctgtt
     2281 ataaagagca tcaaatttat cggcatcaaa cttaatattg atatcgcccc accaaacaga
     2341 attttcagtt tccttgtcgg caacaataaa cttatctttt ggagaacgtc ctgtaaactc
     2401 accggtatct actgcaaggg caccaaagtc tgtaagttca ccttcacctc tgattacaac
     2461 ttcttcaaca agttctgcaa ctgtcagatt ccaataggct gctgctacgt tctttaaacc
     2521 aagttcagca acagaaaagt ttgggtttct taatccgtat tcattcataa tatttcatcg
     2581 tttttatgag gtgcaaagta acaaaaaata aagaaattca agatgactta tagttacttt
     2641 tctgtacaga acttattaac ctgtattttg gaaaaattta gcatttgtaa aaggtttgat
     2701 tcacagttcg ttttaccttt gcccaataat gtaaaaagcg acttcatgtt taaaaaaata
     2761 ttgattgcca acagaggtga aattgcattg cgggttatgc gtactgcaag tgatatggga
     2821 attaaaacgg ttgctgttta tagcgatgcc gaccgcatgg caccacacgt tctatatgca
     2881 gacgaagctg tgcacatagg tgcctcacca tcagcacaat cttacttagt agttgaaaag
     2941 attattcaag ctgcaaaact tacaggagca gaagctattc accccggata tggattctta
     3001 tcagaaaatg ccgatgctgc ccaagctata attaatgccg gtttggtttt catcgggcct
     3061 tcaccgcaat ccatgcggac tatgggtagt aaattagggg caaaagaagc tgctaagaag
     3121 tttaatgtgc ctttagtacc cggaacagac tatgccatta gtgatattga taaagccaga
     3181 caaattgctg ttgatatagg ttttcctgta ttgattaaag ccagtgccgg tggtggtgga
     3241 aaaggaatgc gtattgttga gagtgaaaaa gcctttaatg aatctgtaaa atcagccatg
     3301 agcgaagcac aatctgcctt tggtgacggt agtgttttta tcgaaaaata tattgcttca
     3361 ccccgacata ttgaaattca ggtaatgggc gataatcatg gaaatgttgt tcatcttttt
     3421 gaaagagaat gttcggttca gcgcaggcac cagaaagtaa ttgaagaagc accatcagga
     3481 gttttagacg aaaaaacaag atcagccatg ggccaatgtg cagtaatggt ggctaaagca
     3541 tgtgactatt caggagcagg tacggtagag tttttgcttg atgaaaaaaa taacttctac
     3601 tttcttgaaa tgaatacacg cttgcaggta gaacatcccg taacagaaat gataacagga
     3661 attgatttgg tgagagagca attgaatgtt gctgccggaa ataaattaag tttcagtcag
     3721 gatgatttaa agatacatgg tcatgccgtt gaagtcaggg tttatgcaga agatcctgcc
     3781 aataactttc ttcccagtgt aggtaaacta actacctatg ttccacctag aggggtaggt
     3841 gtaagggttg atgatggtta tgaacaagga atggaaattc caatttatta cgacccgatg
     3901 attgccaagt tagttaccta tgggcgcgac aggaatgagg caatagcaag aatgattaga
     3961 gcaattgatg aatataaaat tacaggctgt gaaaccacct tgccgttttg ccgctttgtg
     4021 atggagcata aagcttttgt tactgcaaaa tttgatacgc attttgtaaa gaaatatttt
     4081 acaccagttg tacttgaaaa aacaatagca gacgaagctg aaatagcttc cttggctgca
     4141 caactgtggc ttgatgaaaa gaagcctgag ggtattcagc aacaacagga aaaatctatt
     4201 tcttactggc gcaacaaccg atcctgaacc aacgtttggt taaacaaatg attaagaaaa
     4261 ttttgaattg taaaatcggc aatgcatttg ctaaaacttc tgtcatgaag ttttcagttt
     4321 ggctttgctt cctgttgacg gcttttatca aattcagcat tgggcaaaca ccggtgggtt
     4381 catcaacagc aatttatgat ggcgatacta ttgcctatta cactttaaat gcagtagata
     4441 tttacggttt tatccctgcc aatgcatctg atgatgtacg caaatacatg aaactgcgca
     4501 gagatgtttt gaaagcatat ccttatgcta aaatcgcttc tgctgaactt agatttattg
     4561 aagagtctgt ttccaaaatg acttcagaaa aggagaagaa aaaattcatt aaagagcaag
     4621 aaaaaatcat gaaagaacag tttgaaaaag atttaaaaaa cctgacttat acgcagggta
     4681 aaatccttat caagttgatt gatcgtgaaa cagggcacac ctcttataat ctcgttaaag
     4741 atttacgtgg ctcacttcag gcatttttct ggcagggtat agctaaaatt ttcagcgcaa
     4801 acctcaagtc tgagtatgac cctgatggcg cagataaaat gattgagaag gtagttcagt
     4861 ctatagaaaa aggcgagctt caggttagaa attaactttt aatttactcc actcaagcgg
     4921 ttttattctg tatctttgcc cggcaaaagc aaataccatg gcacttgcat caaaatcaaa
     4981 cttacaggaa ggattaacct acgatgacgt tttactcgtt cctgctttct cggaaatact
     5041 gccccgcgat gttgatactt caacttcttt ctcaagaaat ataaagctca atgtacccat
     5101 tgtttcagca gcaatggata cggttaccga atctgcaatg gccatcgcaa tcgcacagga
     5161 aggcggcatt ggtgttctgc ataaaaacat gtctattgaa caacaaacca atgaagtaag
     5221 gaaagtaaaa cgttctgaaa gtggtatgat tcatgaccca attactttac atcagaactc
     5281 caccatacac gaagctcttg gcatcatgcg cgaacaacgt attggaggta ttcctattgt
     5341 aaatgacagt aagaagttgg taggcatttt aactaacaga gatcttagat tcgaaacaga
     5401 tgaaagccgt aaggttaaag aagttatgac ttcaggacag ttgattactg taacaggagt
     5461 ggtttcttta caaaaagctg aagtgatttt gcgtcagtat aaaatagaaa aattacctgt
     5521 tgttgaccgt tcgggaaaat taataggatt agttacttat aaagacatca taaaatttaa
     5581 gtcacgaccc aattcatgca aggataaatt agggcgattg agagttgcag caggcattgg
     5641 tgttacagca gatatgcttg atcgtgttgc ctcattggta aatgcaggcg ttgatgccat
     5701 agttattgat actgcccatg gacattcaaa aggtgttatc actgcattga aatctgtaaa
     5761 gaaaaagttt tcaggattag atgttgttgt aggcaatgtt ggaactgctg aaggagccaa
     5821 actacttgtt aaaaatggtg ctgatggcgt taaagtgggt attggtcccg gatctatctg
     5881 cacaactcgt gttgtagcag gtgtcggagt gccgcagcta acagcggtcg ctgatgcggc
     5941 aggtgctata aaaggttccg gtgttcctat tattgctgat ggtggtattc gttttacagg
     6001 cgatattcct aaagccattg cagcaggagc aaacagcatc atggcaggtt caatgtttgc
     6061 aggtgttgaa gaatcaccag gagaaacaat cattttcgaa ggaagaaaat ttaagtctta
     6121 tcgtggcatg ggctccattg aagcaatgcg tgaaggttca aaagatcgtt actttcagga
     6181 tgcagaagat gatattaaaa aattagtacc tgaagggatt gtaggtcgag ttccatacaa
     6241 aggaacactt gctgaaacgg tttatcagtt ggtgggtgga ctaagggcag gtatgggtta
     6301 ctgtggtgca aagaatattg cagaattaca gaaagctaaa tttatacgca tcacacaaag
     6361 cggtgtcaga gaaagccatc cacatggtat tacaattgta aaagaagcac cgaattacac
     6421 tagatagttt ttttgttatc aggtttgatg tggactaatt tgttcgcagt acaattaact
     6481 ttgttttatt aattgacagt gcaagggaaa aaataaagaa gtttatttac taaagcacta
     6541 agaactaaca tgaaattttt aaattggttt tttatcgtgc tttctctctg tattttcaga
     6601 gtattttcgt aacagaatta aaattctatt tcaggaaaat ttgtcttgtt ttaatttacg
     6661 tttatgtaaa aatgactgtt gtatgaattc gtttctgacg aattgactgt tttatattcg
     6721 catttcagta ttggttaaat ttgtgatagt gattcaacaa aaggattaaa atggatataa
     6781 ataaattaac aatcaaatcg caggaagtat tgcagcaagc acagtttctg gctaaatcat
     6841 ttggacagca aactgtagag acaggccatc ttctaaaatc catgcttgag gtagatgaac
     6901 atgttgttcc ttttctatta aaaaaagcaa gtgtaaatat caactcattg acacagaaac
     6961 ttgacaatat cattcaatca tatccaaagg ttagtggtgg tcaggtttat ttatcaaccg
     7021 atgttaacga tgctatgcaa agagcattcg ctgcattaaa agtaaataaa gatgagtttg
     7081 tttctgttga acatttatta ctggcattac tttcaggcaa agacaaagtt gcaaagcttc
     7141 taaaagaaga aggttgtaat gaaaaggact tacaaaatgc aattaacgaa ttgcgaaaag
     7201 gtgaacgtgt gacttcagca agtgcagaag aaacatacaa ttcgttgaat aaatatgcac
     7261 gtaacctgaa tgagttagca cgtaatggta aacttgaccc agtcattgga cgtgatgatg
     7321 aaataagaag agtgcttcag attttgtcaa gaagaacaaa aaacaatcct atcctcattg
     7381 gagaacccgg tgtaggtaaa actgctattg cagaaggttt ggcacataga attatcaatg
     7441 gtgatgtacc tgaaaattta aagtcgaagg ttatttactc attggacatg ggtgctttaa
     7501 ttgcaggagc aaaatacaaa ggcgaatttg aagaacgatt aaaagctgta gtaaaagaag
     7561 tcatcagtgg cgaaggagaa attattttgt ttattgacga aatacataca cttgtaggtg
     7621 ccggtggtgg cgagggtgca atggatgctg ccaacattct gaaacctgca ttggcacgtg
     7681 gcgaactgag ggctgttggt gcaacaactt taaatgagta tcagaaatac attgaaaaag
     7741 acaaagcgct agaaagacgt tttcagaaag tgatggttga agagcctgat acagaatcgg
     7801 caatttctat tcttcgaggg cttaaagaac gttatgaaac acatcataaa gtcagaataa
     7861 aggacgaagc aattattgct gctgttgagc tttcgcaacg ttatattaac gaccgttatc
     7921 ttcccgataa agcaattgac cttgttgatg aagcatcttc aaaactaaga cttgaaatgg
     7981 actctgttcc tgaagaatta gatgagttga acagaagaat catgcagctg gaaattgaaa
     8041 gagaagccat caaacgcgag aacgatacta aaaaagtaaa tgagttatca gaggaaattg
     8101 cacaactcaa cgaacagcga aatgctgtaa aggcacgctg gcagagtgaa aaagaagtgg
     8161 ttgaaaaaat tcaggatata aaagagaaaa ttgaaaatct taaattagaa gcagaacaag
     8221 cagagcgctc cggtgattat ggtagagtag cagaaatccg ttatggcaag gttcgtgaag
     8281 cagaagaaac actttcaaaa ttgatggaac aactcaatga aatgcatagc aaaggtgcca
     8341 tgatgaaaga agaagtggac agtgaagata ttgccgaagt tgtttcacgc tggacaggca
     8401 ttccggtgaa cagaatgtta cagagtgagc gtgagaaact cttgcatctt gaagcagagc
     8461 tgcataaacg tgtagttgga caagatgatg ctattgaggc agttgctgat gctgttagaa
     8521 gaagtcgtgc aggattacaa gatatgcgaa agccaatagg ttcattcata tttcttgggt
     8581 caacaggtgt aggaaaaaca gaacttgcaa aagcattagc tgcttatctg tttaatgacg
     8641 aaaacaacat gactaggatt gatatgagtg agtatcagga gaaacatact gtttcgagat
     8701 taattggtgc gcctcccgga tatgttggct atgatgaagg tggtcagttg actgaagctg
     8761 ttcgcagaag accctattca gtgatattac ttgatgaaat tgaaaaagca catcctgatg
     8821 tatttaatat tctgttgcag gttcttgacg atggcaggct gacagacaat aaaggccgta
     8881 ctgctaattt caaaaacacc atcatcatta tgacatctaa cattggtgca catctgattc
     8941 aggaaaattt tgaaaacatt gatgaaagaa acagagatga agttattgca aagacaaaga
     9001 acaatgtgtt tgatttgctt aagaaaacca tcagacctga atttcttaat cgtattgatg
     9061 aagtgataat gtttaatccg cttacaagaa atcagataga ggagatagtg cgtattcagt
     9121 ttaatcaggc tgttgatatg ttggcaaaga acgacattaa gttatcaatt acagatcgtg
     9181 caatagagtg ggtagctcag ttaggatacg accctcagtt tggtgctaga ccattgaagc
     9241 gtgtactgca aaagaaaatt ctcaacgaac tatcgaaaca gattcttgca ggaacaatca
     9301 ataaagagga tgttattctg attgaccttg acaaaaagaa gaattttatt tttgaaaacg
     9361 aaaagacaca tacagaaata aaagcgtaac aattataata gccgggatta aaagttccgg
     9421 cttttatttt tcaacgaaaa caaaaatatc ttcgtcatct tttttcatca gatatttttc
     9481 tctggcaaac ttttcaaggt ttgcaggaga tgaaatcaat tcgttcaaat cagatttact
     9541 tttttcaagt tcatcagcat aataactttt gtcattcttt aaagtattca actgttgcct
     9601 gaaacgatat tgtgcgtaaa aatcgttctt gtcaaaaaaa gcaagccaca ccacaaatgc
     9661 tataatgcaa attgcatatt tgtttttcaa gaaatcgggt actttttccc acgctttttt
     9721 catgacctaa agttacttgt tcatttaact taaataacct tgttatttac aagttatcag
     9781 aaccaatttg ttaaaagcgt tatttcaaat tgagctgaca aatggtttgt ttttcaataa
     9841 agtattacag tcgctgacta acttttttca ccattaaatt tttccatgaa agaaaacttc
     9901 catcattaat tctttttctt g
//
LOCUS       DAORQT010000039         9855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_887_length_9855_cov_5.615612, whole genome shotgun sequence.
ACCESSION   DAORQT010000039 DAORQT010000000
VERSION     DAORQT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_9855_cov_5.615612"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PK134_02610"
     CDS             <1..873
                     /locus_tag="PK134_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37778.1"
                     /translation="ATQCSIVRGWQDISNHSLGVCTIGDSTSALGMADGLDVVSLGDG
                     GMATLTFANPIMNGSGWDFAVFENSFSETFLELAFVEVSSDGVNFFRFPSVSLTQDTV
                     QVGSFGSIDATQIDNLAGKYSAFYGTPFDLDIMTGISGLDVNHITHVRVIDVVGSIDE
                     LYATYDSQFNKINDPWPTPFPSSGFDLDAVGVIHQNTTSVNSIIENQFNLSYPNPFNK
                     NNVIKLNLTKTEDLSMTLYDISGKAIYQFLDGKINAGEHLFNLRNSLIGNGIYFLKLS
                     SKEHTETFKLIVND"
     gene            866..1900
                     /locus_tag="PK134_02615"
     CDS             866..1900
                     /locus_tag="PK134_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRB37779.1"
                     /translation="MIKQSVFIFLVCFLIFSCKKDKPETIIQPLITSNNNGVVYITNE
                     GNFQFGNAAISLYDKESKNIIEDYYKQVNQVPLGDVCQSMTYFNNKFYIVVNNSSKVM
                     VVNAATFKIEATISGFNSPRYFIPVSNSKAYVSDLYDNRLTIVNLSTNTISGYIPAVA
                     GTEQMKMVYGKVFVSSLFTDKVYVINAMTDMLTDSITLAYSPNSICEDKNGILWVLCS
                     GDSLKQKPSALIQMNPLNNQVIKSFVFTGNHAPWRMCMNQQNDTLYFLDRHVWQMSIS
                     DTTLPSTPFINGNGKLFYTLSVDASNGEIYVSDAIDYLQRGSIYRYSASGNLIDQFKA
                     GIIPGGFCFR"
     assembly_gap    1992..2046
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2054..2129)
                     /locus_tag="PK134_02620"
     tRNA            complement(2054..2129)
                     /locus_tag="PK134_02620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2091..2093),aa:Pro,seq:tgg)
     gene            complement(2221..2309)
                     /locus_tag="PK134_02625"
     tRNA            complement(2221..2309)
                     /locus_tag="PK134_02625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2273..2275),aa:Ser,seq:gct)
     gene            2493..4253
                     /locus_tag="PK134_02630"
     CDS             2493..4253
                     /locus_tag="PK134_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB37780.1"
                     /translation="MAETNSTSGFIAIPDAKAWDPAWSTENKVVYQVLQEPDNLHPTN
                     GKTLMRSEVMLYTQGVLIGTDFKTNSLKPNLLSKLPVYDANKSSYNFHLRNDVQWDNG
                     EHLTANDILFTLKVYKSALTQNDFFKPYLENMLDFIPDVNDSLAFSIKIAKPYLQDVA
                     IWGDIIIMQQSFHDPKNILAKYSLTTLATEDFRANAPADVKKWFEEFNSEATGFDLKK
                     LNGLGPYKVTAWEHGQFLTLEKKNNHWTDHSKDSSEHSSVAKIIFKISSDPNVNVLEF
                     KKQQFDASTTLATKSLLKLREDTLFNHNYNAQFTSTFNYSFAAFNMKPDGKKHKKLFD
                     NLQVRKAMAMLFPYEQINRALYKGEYKRQAGPVCSHKYNFNESLTPILQNYNEATKLL
                     ADAGWKDSDNDGVLDKMIDGIKEDFKFDMNIYAGIPDWNDLSLMLVAELKKAGIVMNI
                     VPLEVPVFLEKARTHDFDMLMSVWTTNVSPDDFTQLWHSSSWRENGDNYSGFGNSASD
                     ALIDSIKFTIDDSLRTALDKRFQKIVVDDMAYIFLFNSQRRIAVHKRFGNVNLYFEKP
                     GLLINTFRLLSPVNKTSATG"
     gene            4335..5696
                     /locus_tag="PK134_02635"
     CDS             4335..5696
                     /locus_tag="PK134_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37781.1"
                     /translation="MLSVYLPGDALDYILIREEKTSITSTNYEHQKTLLAQRLGYDKP
                     LFYFSVISLAEYGWQQYLPSEKQQKSYNHLLRNFGCGSEVFIFYKKINAARTAITTSF
                     ASATDTAIRQTASNAANSLFQIETQVKLSKNDSILDSFNNYATILKLNSLANSILHSY
                     NAMQTKKSSWKKWVPAVQLNAQNRFHDWFFGTDKNSGIIHLNLGQSLYTGRSIGSLLK
                     EKIFWSLLLTVLAIIIAFISGLLISIWLAYTKNKIASRIINMITVLFYSLPVYFAGTA
                     LLYFFSNPDFLQIFPSSGVMPIGGYSSSASFSEKFIGTLPYLILPLICYTYSLWAFVQ
                     RTSASLISAEFKKPYALMAQAKGLTKKEVVIKHIFPNIISPFVALLANMFPAVIGGSV
                     IIESLFSIPGMGLELIHATTTRNYPFISAVILITGLITILLYLLNEWIQHYVDPRTKT
                     VQP"
     gene            5693..6865
                     /locus_tag="PK134_02640"
     CDS             5693..6865
                     /locus_tag="PK134_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37782.1"
                     /translation="MRRKLKFRLAKLWLLTVVLLALFAPFIANEKPFFGNDNLTSDYD
                     LSFGIESKGNNQILRPIPYSPGQPRQNDIALLSPFQKSYYTNNTGQQIELPLRFHHWL
                     GTDAIGADVAAGIVFGLRHSLIIALFSMIFSIVISLIAGICSGYFADSGIYLNRIKFV
                     SLIIFLFLVWFYGFYTFSMEWHNLFQQFSLINLIDLSPSFILFLLLTIALILFWKLSN
                     KRKINHIHLNPDFWISLLTQVMVAVPQLIIIIALSSLFQPSIITLIIIFGSVLWVEPS
                     RMIRAEVQRLRSEGFVDAAIVTGFSFRQIALRHLIPNLIPVVKPVFLYGMAAVILSES
                     GLSFIGLGVNPGTVTLGGLMAQAKENIDAWWLILFPGSCIFFTILSLSALSEKNKS"
     gene            complement(6873..8894)
                     /locus_tag="PK134_02645"
     CDS             complement(6873..8894)
                     /locus_tag="PK134_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRB37783.1"
                     /translation="MKKKLLYISNQHEIVLKFIIFCLTAFLIIKMLPHQVRYKFDYSI
                     SKPWTEKDLIAPFDFAVLKNKDSLNAEKRILLLNATLFFERDTAVKEQVLQRAIARID
                     QIDKHEAELLKQEISILASELYKNGVADESARTVSFGQAKIIKGVDLIPANNFIFEDD
                     AVNELTRIAKEKHIDAPDKAISLLAPLVSNDLTIDKVMTDRFKDELIGTVSETRGMVK
                     KGDLIIAKGEVVTREKYQELESLKSITEEYARPNKHVLIGQVILVVLCLSLLMIFLAQ
                     RRKDIFQSNRRLSIILLMVVFDVFIYTWSLKVEAVSLYIVPLCIMPIVLRTFFDTRLA
                     LFGYIITILILGSIASNGFDFVFTQICAGMITIFSIKDMRNRSHVFWAVLFVYIGYFI
                     CYTSLEIIHAGNMVKVNWENSFWLLANVLLTLFAYPLIYFLERIFKVTSDISLIELAD
                     SNNPLLHELSIKAPGTFQHSLQVANLAESAMFKIGGNALLVRVGALYHDIGKMDMPLY
                     FIENQVTQVNPHDELSFDESAGIIISHVIKGIEKARKYNLPDLVIDFIRTHHGTSRVQ
                     YFYESYLKNYPDKIIDENAFHYPGPKPFSRETAVLMMADSVEAASRSLKKHDSESINK
                     LVEDIIDNQIAHGQFSNCNITFRDITELKKLFKKMLGSIYHVRVEYTVA"
     gene            complement(8933..9326)
                     /gene="rnpB"
                     /locus_tag="PK134_02650"
     ncRNA           complement(8933..9326)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK134_02650"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9454..>9855
                     /locus_tag="PK134_02655"
     CDS             9454..>9855
                     /locus_tag="PK134_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB37784.1"
                     /translation="MSKVAYINGFSAYIKLEKSLSANSNEAYMHDVELLYEFLQEKHP
                     EKNAEKLTLDLLQQFIQWINERNLSARSQARIISGIKAFYKYLLLENIIADDPTTLLE
                     TPKLGRKLPEFLTIEEIDTMLNAIDLSTAEGQ"
BASE COUNT         3134 a         1835 c         1675 g         3156 t
ORIGIN      
        1 gcaacacaat gtagtattgt cagaggatgg caggacattt ctaatcacag tttaggtgtt
       61 tgcacaatag gcgacagcac atctgcatta ggtatggctg atggcctcga tgtagttagc
      121 cttggtgatg gcggcatggc tacattaaca tttgcaaatc caatcatgaa tggtagtgga
      181 tgggattttg cagtatttga gaacagcttt agcgaaacat ttctggaact tgcatttgtt
      241 gaagtgagta gtgatggtgt aaactttttt cgttttcctt cagtgtcttt aacgcaagat
      301 actgtgcagg ttggaagctt cggaagtatt gatgcaacac aaattgataa tctggcaggc
      361 aagtacagtg cattttatgg aacaccattc gacttagata taatgacagg aatttcaggg
      421 cttgacgtaa atcatatcac acatgttaga gtaattgatg tggtgggaag tattgatgaa
      481 ttgtatgcaa cttacgattc gcaattcaat aaaattaatg acccctggcc tactccattt
      541 ccaagttccg gattcgacct tgatgcagta ggagtcattc atcaaaatac aacatcagta
      601 aattcgatta tagaaaatca gttcaatctc tcctacccca atccatttaa taaaaacaat
      661 gtgattaaat taaatcttac taagactgaa gacctaagta tgacattata tgacatttca
      721 ggaaaagcta tttatcagtt tttagatgga aaaatcaatg ccggtgaaca tcttttcaat
      781 cttagaaact ctttaattgg taatggaatt tactttttaa aattatcatc taaagagcat
      841 acagaaacct ttaaacttat tgttaatgat taagcaatct gtatttatat ttcttgtttg
      901 ctttcttatt ttttcctgta aaaaagacaa gcctgagaca ataatacaac ctttaattac
      961 ttcaaacaat aatggagtgg tgtatattac caatgaagga aattttcagt ttggaaatgc
     1021 agcaatcagc ctgtatgata aagaatcaaa aaatattatt gaagactatt acaagcaggt
     1081 aaatcaagtt cctttaggtg atgtttgcca gagtatgact tactttaata ataagtttta
     1141 cattgtagtc aacaattctt ctaaagtgat ggtagttaat gctgcaactt ttaaaattga
     1201 agcaactatt tcaggtttta attcaccacg ttattttatt cctgtcagca acagcaaagc
     1261 ttatgtatcg gatttatacg acaacagact gaccattgta aatctttcta ccaacaccat
     1321 atcaggttat attcctgcag ttgcaggcac agaacaaatg aaaatggttt atggaaaagt
     1381 ttttgtttca tcactcttca ctgataaggt ttatgtcatc aatgcgatga cagacatgct
     1441 gacggatagc ataactttag cttattcgcc caattctatc tgtgaagata aaaacggcat
     1501 attatgggtg ctgtgcagtg gtgattcgtt aaaacaaaaa ccatctgcat tgatacagat
     1561 gaatccgtta aataatcagg ttattaaaag ttttgttttt accggcaacc atgccccctg
     1621 gcgtatgtgt atgaatcagc aaaatgacac tctttatttt cttgatcgcc atgtatggca
     1681 aatgagcatc agcgacacta cattaccttc aacaccattc atcaatggaa acggaaaatt
     1741 attttacact ttgagtgttg atgcttcgaa tggtgaaatt tatgtatcgg atgcaataga
     1801 ctatttgcaa cgtggaagta tttatcgtta ttctgcttca ggaaatttga tagatcagtt
     1861 taaagcagga atcatccccg gaggattttg ttttcggtaa aatttgattc gggatgacta
     1921 agaaaagttg tgatcccgat tggattcgaa ccaatgacct actgcttaga aggcagttgc
     1981 tctatccagc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnntttt ttgtcggggt ggcaggattc gaacctgcga cctcctgctc ccaaagcagg
     2101 cgcgatgaac cgggctacgc tacaccccga aaatttttaa attcggttac gactcaaaga
     2161 acaactttca tgcgaagaaa cattttcttc accttacacc tgattttcgc aaaacttttg
     2221 cggtgagggt gggattcgaa cccacggtac aggttgcccc gtacgacagt ttagcaaact
     2281 gttcctttcg gcctctcagg cacctcacct tttcagatgg gcggcaaagg taaaaaaaag
     2341 tatatgcaaa caaaactata tgctttaatt ttattttttt atgcctctta tatgctaata
     2401 ttgcctttat gagagttgtc ggtatcaaca ttttattcgc atttttgatt ttaatggtct
     2461 cttgcacaga ccacaagccg cagaattaca cggtggctga gacaaacagc acttcaggat
     2521 tcattgctat tccagatgca aaagcatggg atccggcttg gagtacagaa aataaagtcg
     2581 tgtatcaggt acttcaggag cccgacaatc tgcacccaac aaatggtaaa accctgatgc
     2641 gtagtgaagt tatgctttat acgcaaggtg tactgattgg aacagatttt aaaacaaatt
     2701 ctttaaaacc caatctgctt agtaaactac ctgtttatga tgccaacaaa agttcatata
     2761 acttccattt aagaaatgat gttcaatggg acaatggtga acaccttact gccaatgata
     2821 ttctttttac tttgaaagtt tacaaaagtg cacttacaca gaatgatttt ttcaagcctt
     2881 acctagaaaa tatgcttgac tttattcctg atgtaaatga ttcgcttgcc ttcagcataa
     2941 aaattgcaaa accctatctg caggacgttg ccatctgggg tgacattatt attatgcaac
     3001 aatcatttca cgatccaaaa aatattcttg caaaatactc cctgacaact ttagctaccg
     3061 aagattttcg agccaatgca ccggcagatg ttaaaaaatg gtttgaagaa tttaattccg
     3121 aagcaacagg ttttgacctg aaaaaactaa atgggttagg gccatataaa gttactgcat
     3181 gggaacatgg tcagtttcta acattggaga agaaaaacaa tcattggaca gatcattcca
     3241 aggattcatc agaacattct tctgtcgcaa aaataatttt caaaatcagc agcgacccaa
     3301 atgtgaatgt tcttgaattt aaaaagcagc aatttgatgc cagcacaaca cttgcaacaa
     3361 aatcattact caaattgcgt gaggatactt tattcaacca taattacaat gctcagttta
     3421 caagtacatt caactattca tttgccgctt ttaatatgaa gccagatgga aagaagcata
     3481 aaaagttatt tgataattta caagtaagaa aagctatggc aatgcttttt ccctatgaac
     3541 aaataaacag ggcattgtat aaaggcgaat acaaaagaca ggcaggaccg gtgtgcagtc
     3601 ataaatacaa tttcaatgaa tcgcttacac ccattttaca aaactacaat gaagccacta
     3661 aactccttgc cgatgccggt tggaaagata gtgacaacga tggtgtactt gacaaaatga
     3721 ttgatggcat aaaagaagat tttaaatttg acatgaacat ctatgccggc attcccgatt
     3781 ggaacgatct ttctttaatg cttgttgctg aattaaaaaa agcaggtata gtgatgaata
     3841 ttgttcctct tgaagtacct gtgtttttag agaaagcgcg aacacatgat ttcgatatgc
     3901 ttatgagtgt gtggacaaca aatgtttctc ctgatgattt tacgcagcta tggcattcgt
     3961 caagctggcg cgaaaatggt gacaactatt caggatttgg caacagtgct tcagatgcat
     4021 tgattgattc aataaaattt accattgatg attctttgcg gactgctttg gacaagcgtt
     4081 ttcagaaaat agtggttgat gatatggcgt atatttttct ttttaattca caacgcagaa
     4141 ttgcagtgca caaacgattc ggaaatgtaa atctttattt tgaaaaaccc ggattactca
     4201 tcaatacttt tcgattactt tcaccggtta acaaaacttc tgcaacagga taaataaaat
     4261 tcagactaag gaagatgcgg catatcattt tcaaatactt ttttattttt tctgtactgt
     4321 tggtttcatc gtttatgcta tctgtttatt tgcccggtga tgcattggat tacattctca
     4381 tacgagaaga aaaaacaagt attacttcaa ctaattatga gcaccagaaa acattacttg
     4441 cacaacgtct aggctatgac aagcctttat tttatttttc tgtaatatcc ttagctgaat
     4501 atggatggca acaatatctt ccttcagaga aacaacaaaa aagctacaat catttgttgc
     4561 gaaattttgg ttgtggtagt gaagtattta ttttttacaa gaaaattaat gctgcaagaa
     4621 cagcaattac aacatcattc gcttcagcaa ctgatactgc aatcaggcaa actgcttcta
     4681 atgcagcaaa tagtttattt cagatagaaa ctcaagtaaa actgagcaag aatgacagta
     4741 ttcttgattc tttcaacaat tatgcaacta tacttaaact taattcactc gctaattcta
     4801 ttttgcattc ctataatgca atgcaaacta aaaaatcatc atggaaaaaa tgggttcctg
     4861 cagttcaact taatgcacaa aatcgttttc atgactggtt ttttggaaca gacaagaata
     4921 gtggcatcat acatttaaac ttaggtcaat cactctatac cggaagaagc attggctcat
     4981 tactgaaaga aaaaatattc tggtcattgc ttctgactgt tctggcaatt atcatagcat
     5041 ttatttccgg ccttttaatt tcaatatggt tggcatatac aaaaaacaaa attgccagcc
     5101 gcattatcaa tatgattacc gtactttttt attctctacc ggtctatttt gcaggaacag
     5161 ccttactata ttttttctct aatcctgatt tcttacaaat atttccatct tcaggagtta
     5221 tgcctattgg aggatattct tcaagcgcaa gtttttcaga aaaatttatc ggcacccttc
     5281 cctatcttat ccttccgcta atttgctaca cctattccct gtgggcattt gttcaacgta
     5341 catctgccag cctaatttca gctgaattta aaaaacctta tgccttaatg gcgcaagcaa
     5401 aagggctaac aaaaaaagaa gttgttatca aacacatttt tccaaatatc atatcgccat
     5461 ttgtagcctt gcttgcaaat atgtttccag cagttattgg cggttcggta attattgaat
     5521 cattgttttc tattccgggt atgggacttg aactgattca tgctactacg acaagaaact
     5581 atcctttcat ttctgctgtt attttaatta caggactaat tacaatttta ctttatctgc
     5641 ttaatgaatg gattcagcat tatgttgacc cacgaactaa aactgttcag ccatgaggag
     5701 gaagttgaaa tttcgtttag caaaactttg gctgctgaca gttgtactcc ttgcattgtt
     5761 tgctccattt attgctaatg aaaaaccatt ttttggaaat gacaatctaa cttctgacta
     5821 tgatttatca ttcggaattg agagcaaagg caataatcaa attctgcgcc ctatacccta
     5881 ctcacccggt cagccaagac aaaatgatat agcgttatta agtccgtttc aaaaaagtta
     5941 ttatacaaac aatacaggac aacaaataga attacctctg cggtttcatc attggttagg
     6001 tacagatgca attggtgctg acgttgctgc aggaattgtt tttggtttac gacacagttt
     6061 aattattgcc ttgttttcaa tgatttttag tattgtaatt tcattgattg caggtatctg
     6121 ttcaggatat tttgctgact ctggaattta tttaaataga ataaaatttg tttcactaat
     6181 aatatttctg tttctggtct ggttttatgg cttttacaca ttctcaatgg aatggcacaa
     6241 cctgtttcag caatttagct taatcaattt aatagactta tcaccttcct ttattctatt
     6301 tctgctttta acaattgctt taatattatt ttggaagtta tctaataaaa gaaaaatcaa
     6361 tcatatccat ctcaatcctg atttttggat ttcattgctg acacaagtca tggtagccgt
     6421 accacaactt attatcatca ttgcactctc atcattgttt caaccatcta tcatcacatt
     6481 aatcataatt ttcggatcgg tgctttgggt tgaaccatca cgcatgatca gggcagaagt
     6541 acagcgactt cgtagtgaag gctttgttga tgctgctatt gttaccggat tttcattcag
     6601 acaaattgct ttgaggcatt taatccccaa tttaattcct gttgtaaaac ctgtattctt
     6661 atatggaatg gcagccgtta ttttgagtga gtcaggatta tcatttattg gattaggcgt
     6721 aaatcccgga accgttactt taggtggact gatggctcag gcaaaagaaa atattgatgc
     6781 ctggtggctg atactttttc cgggctcatg tattttcttt acaattttga gcttgtcagc
     6841 cttaagcgaa aaaaataaat cttgacattt tatcatgcta ccgtatattc aacacgtaca
     6901 tgatagatac ttccaagcat tttcttgaat aattttttca actcagtaat atctctgaaa
     6961 gtaatattac agttagaaaa ttgtccgtgt gcaatttggt tatctataat atcttctacc
     7021 aacttattga tactttcgga atcatgcttc ttaagacttc gtgatgcagc ttcaacagaa
     7081 tccgccatca tcaatacggc agtctctctg ctgaaaggtt ttgggccggg ataatgaaat
     7141 gcattttcat caataatctt gtcaggataa tttttaagat acgactcata aaaatactgt
     7201 acacgtgagg ttccgtgatg tgttcttata aagtcaataa ccagatcggg aagattatac
     7261 tttctggctt tttctattcc tttaatcaca tggctgatga tgatacccgc actttcatca
     7321 aaagacaatt catcgtgtgg atttacttgc gtaacctgat tctcgataaa ataaagcggc
     7381 atatccattt taccaatgtc atgatacaat gcacctactc tgaccaacaa tgcattgcca
     7441 ccgattttaa acattgcaga ttcagcaaga ttagcaacct gcagtgaatg ctgaaatgtt
     7501 ccgggagctt ttatactcaa ctcatgtaac aatggattgt tactgtctgc aagttctatc
     7561 aaagaaatat cagaagtcac tttaaaaata cgctcaagga aatatattaa tggataagca
     7621 aaaagtgtta acaacacgtt agcaagcaac cagaaagaat tctcccaatt tactttaacc
     7681 atatttccgg catgaataat ttcgaggctg gtataacaaa taaaataacc gatataaaca
     7741 aacaacactg cccagaaaac atgagagcgg ttgcgcatat cttttatact gaaaatagtt
     7801 atcataccgg cacagatttg tgtaaacaca aaatcaaaac cgtttgatgc aattgatcct
     7861 aatattaaaa ttgtgatgat ataaccaaac aatgcaagtc gtgtatcaaa aaatgtacgt
     7921 aacacaattg gcataataca gagaggaaca atatataaac tcacagcttc aactttcaac
     7981 gaccatgtgt atataaatac atcaaaaact accattaata aaataatact tagccttctg
     8041 tttgactgaa aaatatcttt tcttctttgc gcaagaaata tcatcaataa agaaagacac
     8101 aatacaacca atattacttg accgataagt acatgtttat tgggacgagc atattcttca
     8161 gtaattgatt tcagagattc caactcctga tatttttcgc gtgtcacaac ttcacctttg
     8221 gcaataatta aatcgccctt tttaaccatt ccgcgcgttt cagaaaccgt accaatcaat
     8281 tcatctttaa aacggtctgt cattacttta tcaatggtca aatcattact caccaaaggg
     8341 gcaagtaaag aaatagcctt gtcaggagca tcaatatgtt tttctttagc aatacgcgtc
     8401 agctcattaa cggcatcatc ttcaaaaata aaattgttag caggtatcag gtcaacacct
     8461 tttattattt tagcttgacc aaaggagaca gtacgtgcac tttcgtcagc tacaccattc
     8521 ttataaagtt cactggcaag tattgaaatt tcctgcttca ataactctgc ttcatgttta
     8581 tctatttgat caatacgtgc tattgcccgt tgcaaaacct gttccttaac ggcagtatca
     8641 cgttcaaaaa acagtgttgc attgagaagt aaaattctct tctctgcatt cagcgaatct
     8701 ttattcttca gaacggcaaa atcaaacggt gcaatcagat ctttctctgt ccagggttta
     8761 gatatagaat aatcaaactt ataccttacc tgatgtggca acatttttat tatcaaaaat
     8821 gctgtaagac aaaagataat aaatttaaga actatctcat gctgattgct gatgtaaagg
     8881 agcttctttt tcattggctg ctaaattatt aaaaagcaat gcagcagaaa aaataaaaag
     8941 caagcctgta agccggattc tgttatctga atacttttca gcattcaaat cccctatcat
     9001 ttgtctgttt gcggcatacc cttcacgaag atgttcattg ctgaacaaac ggcaacgagc
     9061 cattgcctgt tcgtgattta ttttgccttt caactcctga ggtttacctt gcattttcat
     9121 tactgaatat gcccgtaagc tcttacctca cgttttcacc tttaccacca tgcaatacat
     9181 tgccggtgga attattttct ctgtggcact ttctgttata gcttcatcac taaaactaca
     9241 ccctccttta acaggaggca ggatactctt tgttgcccgg actttcctct cttaaacgag
     9301 ttaagagcga tagagcggct tgccgatgca aaaatacggt ttattatcct tgattcttag
     9361 ttaaaatcaa taaacatgca atagttgatt ttttatactt aattacacca aaccaaactc
     9421 ttgagttaag tttcggttag ctattttagc acaatgagta aagtggcata cattaatggc
     9481 ttttcagcat acattaaatt ggagaaatca ttatctgcca attctaatga agcctatatg
     9541 catgatgtgg aactgcttta tgaatttctt caggaaaaac acccggaaaa aaatgctgaa
     9601 aagctcacat tagacctctt acaacaattt attcagtgga taaatgaacg gaatcttagc
     9661 gctcgttcac aggcaagaat tatttcaggt attaaagcat tctataaata tctcttactc
     9721 gaaaatatta ttgctgacga cccgacaaca ttattagaaa cacctaaact tggcagaaaa
     9781 cttcctgaat ttttaactat tgaagaaatt gacaccatgc taaatgctat tgatttaagt
     9841 acagcagaag gtcag
//
LOCUS       DAORQT010000040         9836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_891_length_9836_cov_8.727124, whole genome shotgun sequence.
ACCESSION   DAORQT010000040 DAORQT010000000
VERSION     DAORQT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_9836_cov_8.727124"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK134_02660"
     CDS             complement(<1..202)
                     /locus_tag="PK134_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRB37785.1"
                     /translation="MCAIQDALAHHLKIVLYQYLNFQDVQARHYITRIESIKRILPDC
                     GISTDIIAGFCSETEEEHQDTIS"
     gene            253..417
                     /locus_tag="PK134_02665"
                     /pseudo
     CDS             253..417
                     /locus_tag="PK134_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            417..959
                     /locus_tag="PK134_02670"
     CDS             417..959
                     /locus_tag="PK134_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRB37786.1"
                     /translation="MSNIIMISGLLKRKLFSGLIVLIAFTVMMESCGVYSFTGASITP
                     GVKTVSIQYFPNRASVAQPSLSQVFTDKLKEKFVSQTSLIQVNQSGDLRFEGYISDYT
                     VQPTAIQSTDVAALNRLTITVHVKFENMKDEKQNFETSFSRYADFDSRKDLTSVESSL
                     MNDISSQLVDDIFNKSVVNW"
     gene            959..2386
                     /locus_tag="PK134_02675"
     CDS             959..2386
                     /locus_tag="PK134_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37787.1"
                     /translation="MTKERFFNFIQNPLSVHANDIEELEQIIEQFPYFQTARLIYTKS
                     LHNENSYLYNDELKRTAAFAADRSVLYKLIHSIKKDVTIDEPESLITEDIGSKSVINP
                     VQEVPDLNVFVFEEKTEIEEQAIVFEEKENSESADDELIIFEEKTLTIEEGEPIVFEE
                     KRPEITTQEDIVFTAVDEPINASAEDDEVGSSNKIAEIESEKKERVLTPAEILSQRLR
                     EIESQLEDKKEDETEVVSELKEETIIPETPQIAEPLNIQSEVLPESIQISSSIPEIKE
                     EVAEISEQAEAEKLIPAEPESSEKTIEVVAISPQIQPPVIEEVVAPDAIVQAEIKPLP
                     EADKKEKHSFTDWLHRFHPQEEKKTLSPDNLEEDNYNNKLINSQLTSYIETKNSNNEI
                     VNQFIQNEPRIDSSKTKFFSPGNMAKSSVTDVSDIVSETLAKIYLGQNNFSKAIQTYE
                     KLMLKYPEKSVYFAALIKEIKKSQI"
     gene            2399..2764
                     /gene="secG"
                     /locus_tag="PK134_02680"
     CDS             2399..2764
                     /gene="secG"
                     /locus_tag="PK134_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRB37788.1"
                     /translation="MYTLITVLIILISVLLCLAVLVQNSKGGGLASGFSAPQQIMGVR
                     KTTDFLEKFTWGLGTALIVFCVVANFMTPGSNNSTEVRASSIQEQIDNATLPAAPQAP
                     LPSQQNAQPAQQPAAQPAN"
     gene            2947..3225
                     /gene="groES"
                     /locus_tag="PK134_02685"
     CDS             2947..3225
                     /gene="groES"
                     /locus_tag="PK134_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRB37789.1"
                     /translation="MSKISLKPLADRVIVEVAAAEEKTASGIIIPDTAKEKPQKGKVV
                     AVGGGKKDEPMTVKVGDSVLYGKYAGTEISVEGKEYLIMRESDIFAIV"
     gene            3279..4910
                     /gene="groL"
                     /locus_tag="PK134_02690"
     CDS             3279..4910
                     /gene="groL"
                     /locus_tag="PK134_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611460.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRB37790.1"
                     /translation="MAKEITFNLDARDALKKGVDALANAVKVTLGPKGRNVIIDKKFG
                     SPTITKDGVTVAKEIELTNSIENMGAQMLKEVASKTADIAGDGTTTATVLAQSIVNTG
                     LKNVTAGANPMDLKRGIDKAVEAVVASLKKMSKEVGDDNKKIEQVATISANNDSEIGK
                     LIAEAMAKVKREGVITVEEAKGTETTVEIVEGMQFDRGYISPYFVTNADKMQAVLESP
                     YILLYDKKVSNMKELLPILEKVVQTGKPLLIIAEDVDGEALATLVVNKIRGSLKIAAV
                     KSPGFGDRRKAMMEDIAILTGGTLIAEEQGFKLENADLSYLGTCEKITIDKDNTTIVG
                     GAGKKADITARVNQIKAQIETTTSDYDKEKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVAYIRAITALDKLKGDNDDETTGIAIIKRALEEP
                     LRQIVSNAGIEGSIVVQKVKEGKDDFGYNARSDKYENMHAAGVIDPTKVTRVALENAA
                     SVAGMLLTTECVLADIKEDKPAMPAMPGGMGGMDY"
     gene            complement(5189..6880)
                     /locus_tag="PK134_02695"
     CDS             complement(5189..6880)
                     /locus_tag="PK134_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRB37791.1"
                     /translation="MKNLILTGLTIGLTCMMSYAQKYDEMKTTWEIETTHPFTETGLS
                     EKKEIVHGSNEKMFTMVSAVDGKVKWSKKFKEIDEQIGKVDLQIPMYESNAVFLFDKK
                     AGKDQMVVVDMETGTKLWSTNKYQGIDDPDEVIYIAELNAYAIVTKTNLTMVKARTGE
                     ELWSTVKFSTPVGKYFYDEKENAIVIISMPRNAWQAMFKGFRNQILKINAKNGDILWE
                     QTFVGIVERKVVTRESVAKIFLRDDKLFLQLNGLQVYEYKTGSPIWAAAYDVTFAREL
                     VRPKVYGQVVKYGVYDAIAQPLYDGEYVYILDVKSRKSQYLKKYEINSGKLVWSSPEI
                     KDARVIPGLYKIDDRIIVQIGGAVEVQAISKETYGNGYVQWTQYYRTVKYQNVKPYEV
                     QAFSTSDGTKSWDSERFRKGITNIFPSGNNIVVSSGKALYSLDYKTGKEAYEIPLGDD
                     DIGLAEKIVNPTELGDNISKDNVIVLGEKGVSSHNIATGAKVWAVKTKKGEFSGIFGE
                     NIFFDTEKGDYFSIDANSGKAKFFDSRKNSKSIVSLDGKSIYVFEGKKILKVSTE"
     gene            complement(6922..8433)
                     /locus_tag="PK134_02700"
     CDS             complement(6922..8433)
                     /locus_tag="PK134_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37792.1"
                     /translation="MKPILLAILSLAFVTTVYAQPAGTRTITIDDCNYPILGIETNHA
                     SDKIVLTASQGRIFFINVSDVEANTEVNPVWKNLNLSGLNNGGKPQFSENDQYILLQE
                     SSALKTAITSRKVKDIKYYILNAADGKVVSEGNNVNSVQFLSNSTQILIASNDGIMVK
                     DFLSNKVIAQLEVDNCEMAAISHDNKLIALSFDPDKADFKEVESIGKNKSELKTARKN
                     KRLISFYSYPDLKKLYNSKEEIDIVYSIKFSRDDKQTYLLSHNVVAERIGTAGLNGIS
                     MAAADNRRQTNIQVLDASNGNLVKEIYHATAEPDADYKFSTYSNYFGYSDNDGFIGWK
                     RKLVLIDNNDHKNKVGQYRYQGRRGSSNIFPIYFSFNKTKPVAYLSNGTKLIEWNYET
                     LKEYTDYIEGQDNDSLQSKAISQLNADLENPESDLSKNIVKQNIKGIYLFDLTVQKKG
                     QIVTIFAESDDKTDIPHQNALKDLLLKYKFKDIVVSPNQRLKFRYTFSIENNI"
     gene            complement(8870..9211)
                     /locus_tag="PK134_02705"
     CDS             complement(8870..9211)
                     /locus_tag="PK134_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37793.1"
                     /translation="MEMEQLSNQNFQQRNLIEQTVVQLNKDLIPCGFTVAWSGDTFSA
                     YQDIIMQLEVIIRQLAKSGSSTLNAWLYRVDIPEKTLKKVLNKQDNQQALAQAILERT
                     FIKIMFRNSYR"
     gene            complement(9227..9652)
                     /gene="aroQ"
                     /locus_tag="PK134_02710"
     CDS             complement(9227..9652)
                     /gene="aroQ"
                     /locus_tag="PK134_02710"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRB37794.1"
                     /translation="MAKRIVIINGPNLNLLGKREPEIYGHQTFDDFAITLKKEFPEPN
                     IQIFQSNVEGEIINLLHNEGFKADGIIINAGGYTHTSVAIADAIAAIPSPVVEVHISN
                     IHSREEYRHKSLLAANCKGVITGFGLNSYRLALLALLNS"
BASE COUNT         3273 a         1738 c         1741 g         3084 t
ORIGIN      
        1 ggctgatggt atcctgatgt tcttcttctg tctcactgca aaagccggca ataatatctg
       61 tgctgatacc acagtcaggc aaaattcttt ttatactttc tattcttgta atgtaatggc
      121 gagcctgtac atcttgaaaa ttcaggtatt gatacagaac gatttttaaa tgatgtgcaa
      181 gggcatcctg tattgcacac aaacgatact gcaattacac ttacaaaacc cacacatata
      241 gatgtaaaac cacatgagca tattgaagaa tcgctgagca ttgccgacag agaaaaagat
      301 ctgattatac gggcattgga aaagtacaga ggtaaaagaa aaggtgcagc aaaagaatta
      361 ggaatttcgg agcgaacgct atatagaaaa attaacgaat acggcattaa agactgatga
      421 gtaacataat aatgatttca ggtttattaa agcgtaaatt attctcagga ttaattgttc
      481 tgattgcatt tactgtaatg atggaatcgt gcggtgttta ttcttttacc ggtgcatcta
      541 ttacgcccgg agtaaaaaca gtaagtattc agtattttcc taacagagca tcggtagcgc
      601 agccatcatt aagtcaggtt tttactgata aactcaaaga aaaatttgtt tcacaaactt
      661 cgctcattca ggttaatcaa tcgggcgatt tacgttttga aggatatatt agtgattaca
      721 ctgttcagcc tactgcaata caaagtacag atgtggctgc actaaaccgc ctcaccatta
      781 ccgttcatgt taaatttgaa aacatgaaag atgaaaaaca gaattttgaa actagttttt
      841 cgagatatgc cgactttgat tccaggaaag atctgaccag tgttgaatca tctcttatga
      901 atgatatatc ttcgcagctt gtagatgata tttttaataa atctgttgtt aactggtaat
      961 gactaaggaa cgttttttta atttcattca aaatccgtta agcgttcatg ccaatgacat
     1021 cgaagagttg gaacaaatca ttgagcagtt cccctatttt cagactgcca ggttgattta
     1081 taccaaatca ctgcacaatg aaaacagtta tttgtacaat gatgaattaa agcgaactgc
     1141 tgcctttgct gccgacagga gtgttcttta taaactcatt cattcaatta aaaaagacgt
     1201 tacaattgat gagccggaaa gtttgattac agaagatata ggatcaaaat cggtaattaa
     1261 tcctgttcaa gaagtacctg atttgaatgt ttttgtattt gaagaaaaaa cagaaattga
     1321 agagcaggca attgtatttg aagaaaaaga aaattctgaa tcagcagatg atgaactcat
     1381 aatatttgaa gaaaagacat taacaattga ggagggcgag ccgattgttt ttgaagaaaa
     1441 gcgaccagaa attaccacac aggaagatat agtttttaca gcagttgacg aacccatcaa
     1501 tgccagtgca gaagatgatg aagttgggtc ttcaaataaa attgcagaaa tagagtcaga
     1561 gaaaaaagag cgtgtgctga cacctgcaga aattttatct caaagactac gagaaatcga
     1621 atctcaatta gaagacaaaa aagaagatga aactgaagta gtctctgaat tgaaagaaga
     1681 aacaattatt cctgaaacac ctcaaattgc agaaccttta aatattcaat ctgaagtttt
     1741 gccggaaagc attcaaatta gttcttctat accggaaatt aaggaagaag tagcagagat
     1801 ttctgaacaa gcagaagcag aaaaactaat acccgctgaa cccgaaagtt cagaaaaaac
     1861 aattgaagtt gttgcaataa gtcctcaaat tcaaccaccc gtaattgaag aagttgttgc
     1921 acctgatgca atagttcaag ctgaaataaa accattgcca gaggctgata aaaaagaaaa
     1981 acattcgttt acagactggc tgcatcgttt tcatccacag gaggaaaaaa aaactttaag
     2041 ccctgataat ttagaagagg ataactataa caacaaactg attaacagtc aattaacgtc
     2101 atacattgaa actaaaaatt caaataatga aatagttaat cagtttatcc aaaacgaacc
     2161 caggattgac agtagcaaaa ctaagttttt cagtccgggc aatatggcca agtcaagtgt
     2221 tactgatgta tctgacatag tttcagaaac gctggcaaaa atatatttag ggcaaaacaa
     2281 tttcagcaag gctattcaaa catacgagaa gctgatgttg aaatatccgg aaaaaagtgt
     2341 ttactttgca gcccttatta aagaaataaa aaaatcacag atttaacatt caatctatat
     2401 gtacacttta attaccgtgt taattatttt aattagtgtt ttactttgcc ttgccgtatt
     2461 ggtacaaaac tcaaaaggtg gcggtttagc ttccggattt tctgcaccac agcaaattat
     2521 gggcgttcgt aaaacaacag actttcttga gaaatttact tggggattag gaactgcttt
     2581 aatcgttttc tgcgttgtag caaattttat gactccaggc agtaataaca gtactgaagt
     2641 aagagcttct tctatacaag agcaaattga caatgcaacg cttcctgctg caccacaggc
     2701 accattgcca tcgcagcaaa atgcacaacc tgcacagcaa cctgctgcac agcctgcaaa
     2761 ttaatcttgt cagttaataa cttttaaagg tgccataatg ctgaaattat gacaccttta
     2821 ttatttaagc caaagccata aatcgcaaaa ctgtcagtaa aatagtcatg gcacagaaac
     2881 tgataaaggc aaatagaaat taattataaa gtataaaagt caaacatttt aaatcacatt
     2941 attactatga gtaaaattag tttaaaacca ttggcagacc gcgttattgt agaagtggct
     3001 gcagcagaag aaaaaacagc aagtggaatt attattcctg acacagcaaa agaaaaacct
     3061 caaaaaggca aagtagtagc tgttggtggt ggcaaaaaag atgagccaat gactgtaaaa
     3121 gttggtgaca gcgtattgta cggaaaatat gcaggaacag aaatttctgt tgaaggcaaa
     3181 gagtacctta tcatgcgtga atcagacatc ttcgcaattg tttaaaagct ttagaaaaca
     3241 aaaagaaata aataaacaac ttaaataaaa tacgaaaaat ggcaaaagaa atcacattta
     3301 accttgatgc acgcgatgct cttaaaaaag gtgttgatgc attggcaaat gcagtaaaag
     3361 taactttagg acctaaaggc cgcaacgtta ttattgataa aaaattcgga tcaccaacaa
     3421 ttacaaaaga tggtgttacc gttgcaaaag aaatagaact cacgaacagt attgagaaca
     3481 tgggtgctca gatgctgaaa gaagtagcgt caaaaactgc tgacattgca ggtgatggta
     3541 caacaacagc tacagtactt gctcagtcaa ttgtaaacac aggtttaaag aacgttactg
     3601 ccggtgccaa tccaatggat ttaaaacgcg gtattgacaa agctgttgaa gctgtagttg
     3661 catcattaaa gaaaatgagc aaagaagttg gtgacgacaa taagaaaatt gagcaggtgg
     3721 ctacaatttc tgcaaacaac gatagcgaaa taggaaagct tattgctgaa gcaatggcta
     3781 aagtaaaaag agaaggtgtt attactgttg aagaagcaaa aggtactgaa actacagttg
     3841 aaattgtaga aggtatgcag tttgacagag gatatatctc tccatacttt gttaccaatg
     3901 cagataaaat gcaggctgtt ttagaaagcc catacatttt attgtacgac aaaaaagtat
     3961 ctaacatgaa agaattactt ccaatattag agaaagtagt tcagacagga aagcctttgt
     4021 tgattattgc tgaagatgta gatggcgaag cattagctac attggtagta aacaaaatcc
     4081 gtggctcact gaaaattgca gcagtaaaat ctcctggctt tggcgatcgc agaaaagcaa
     4141 tgatggaaga tattgcaatc cttacaggcg gcacattaat cgctgaagag caaggattca
     4201 aattagaaaa tgctgacctc tcctacttag gcacatgcga aaaaatcact attgataaag
     4261 acaacacaac aattgttggt ggtgcaggaa aaaaagctga catcactgct cgtgttaatc
     4321 agattaaagc acagatagaa actacaacat ctgactacga caaagaaaaa cttcaggagc
     4381 gtttggcaaa attagcaggt ggtgttgctg tactttatgt tggtgcagct acagaagtag
     4441 aaatgaaaga aaagaaagac cgtgttgacg atgcattaca tgctacacgt gcagcagttg
     4501 aagaaggtat tgttgcaggt ggtggtgttg catacattcg tgcaataaca gctttagaca
     4561 aacttaaagg tgataacgat gatgaaacaa caggaattgc aatcatcaaa cgtgcacttg
     4621 aagagccatt acgtcagatt gtgagcaatg ccggaattga aggaagcatt gtagtacaaa
     4681 aagtaaaaga aggcaaagac gactttggtt acaatgcccg ctcagacaag tatgaaaaca
     4741 tgcatgctgc cggagttatt gatcctacta aagtaacacg tgttgctctt gaaaatgcag
     4801 cttctgttgc tgggatgtta ctcactacag agtgtgtact tgccgacatt aaagaagata
     4861 agcctgcaat gcctgcaatg ccgggtggaa tgggcggaat ggattattaa tcatctgcaa
     4921 tttcaaagta cggaaaacgt atttcggaac aataaacaaa accctctgtt ggattttacc
     4981 ttcagagggt tttttatttg tcagttgtaa ggtttagaaa gttggaatgt ttggaaaatt
     5041 ggctttactc tataatcaaa catataataa ttctactgtc ctcactaagt tcctatctta
     5101 cctactccgc tattttgcta taatccaaaa taaaaaagtc ccgacatttt tttgccggga
     5161 cttttttata aataatttaa aactttcttt attcagtaga tactttcagg attttctttc
     5221 cttcaaacac ataaatgctt tttccatcta aagaaacaat agattttgaa tttttgcgtg
     5281 agtcgaagaa ttttgcttta ccggaattgg catcaataga aaaataatcg cctttctctg
     5341 tatcaaagaa aatattttct ccgaaaattc ctgaaaactc tccttttttg gtttttactg
     5401 cccacacttt tgcaccagta gcaatgttat gagaagaaac gcctttctct cccaaaacaa
     5461 ttacattgtc tttactaata ttatcaccta attcagttgg atttacaatt ttttctgcca
     5521 agccaatatc atcatcacct aaaggaattt cgtaggcttc tttgcctgtt ttgtaatcta
     5581 aagagtataa tgccttacca cttgacacta caatgttatt gccgcttggg aatatatttg
     5641 taattccttt cctaaagcgc tcagagtccc aagattttgt accatctgaa gtactaaacg
     5701 cctgcacttc ataaggcttt acattctgat attttacagt tctgtaatat tgtgtccatt
     5761 gtacatagcc gttaccataa gtttcttttg atatagcctg cacttcaaca gcgcctccaa
     5821 tttgcacaat tattctatcg tcaattttat acaagcccgg aataactctt gcatctttaa
     5881 tttcaggaga agaccaaaca agtttacctg agttgatttc gtatttcttt aaatactgac
     5941 tttttctgct ctttacatct aaaatatata cgtactctcc gtcatacaat ggttgtgcaa
     6001 tggcatcata aacaccatat tttacaacct ggccatatac tttgggcctc actaattctc
     6061 ttgcaaaagt tacatcgtaa gctgctgccc aaattggact tcctgtttta tattcataaa
     6121 cctgcaagcc atttaattgt aaaaacaact tatcgtctct taaaaatatt ttagcaacag
     6181 attcgcgggt aacgactttt ctttctacta ttccaacaaa ggtttgctcc cataaaatat
     6241 caccattctt agcgttgatt ttcagaatct gatttctaaa acctttaaac atggcctgcc
     6301 atgcatttct gggcatgcta atgatgacaa ttgcattttc tttttcatca taaaagtatt
     6361 taccaaccgg tgtactaaat ttaactgttg accaaagttc ctctccagtt ctggctttta
     6421 ccatggttaa atttgttttg gttacaatag cataagcatt taattctgca atgtaaataa
     6481 cctcatccgg atcgtcaatg ccttgatatt tattggtact ccaaagttta gtacctgttt
     6541 ccatgtcaac caccaccatt tggtctttac ctgctttttt atcaaataaa aacactgcat
     6601 tcgattcgta cattggaatt tgtaaatcta ctttcccaat ttgctcatca atttctttaa
     6661 actttttact ccattttact ttaccgtcaa cagcggaaac catggtaaac attttttcat
     6721 tgctaccatg tacgatttct ttcttttcgc tcaatccggt ttctgtaaac ggatgtgtgg
     6781 tttctatctc ccacgtggtt ttcatttcat cgtatttctg agcgtagctc atcatacagg
     6841 tcagacctat tgtcagaccg gttaaaatta gatttttcat tttttattgt ttgttttaat
     6901 taattaaaat ttatcttttt gttatatgtt attttcaata ctaaaggtgt atctgaattt
     6961 cagtcgttga tttggagaaa caacaatgtc tttaaattta tatttgagta acaaatcttt
     7021 taaagcattt tgatgaggga tgtcagtttt atcatccgat tcagcaaaga tggtaactat
     7081 ctgtcctttt ttctgaacag ttaaatcaaa taagtatata ccttttatgt tctgctttac
     7141 tatatttttt gacaaatcac tctccggatt ttctaagtcg gcattaagct gactaattgc
     7201 ttttgattgt aaggagtcgt tatcttgtcc ttcaatataa tcagtatatt cttttaaggt
     7261 ttcataattc cattctatca gctttgtgcc attggataag taggctacag gttttgtttt
     7321 attaaaggaa aaataaatag gaaaaatatt tgagcttcct ctcctgcctt ggtaacggta
     7381 ttgcccaact ttgtttttat gatcgttatt atcaattaat acgagtttac gcttccatcc
     7441 aataaatccg tcattgtctg aatatccgaa ataattagag taagtactaa acttgtaatc
     7501 ggcatcaggt tcggcagttg catgataaat ttctttaact aagttaccat tgcttgcatc
     7561 taaaacctga atattagttt gtcttctatt atcggcagca gccattgata tgccatttaa
     7621 acctgctgta ccaatgcgtt cagctactac gttatgcgag agtaaataag tttgcttatc
     7681 atctctgctg aatttaatag aataaacaat gtctatttct tcttttgagt tataaagttt
     7741 ctttaaatcg gggtagctgt aaaagctgat tagtcgcttg ttctttctgg ccgtttttaa
     7801 ctcactttta tttttaccaa tagattctac ttctttaaag tcagctttat cgggatcaaa
     7861 agaaagtgca attaatttat tgtcgtggct gatggctgcc atttcgcaat tatctacttc
     7921 taattgagct attactttgt ttgataaaaa gtctttcacc ataatgccat catttgatgc
     7981 tattaaaatt tgtgtgctgt tgcttaaaaa ctgtactgag tttacattgt ttccttcaga
     8041 aacaactttt ccgtcagcgg catttaaaat ataatatttt atatctttta cctttcttga
     8101 ggtaattgca gtttttaaag ctgacgattc ctgcaataat atatattggt cgttctcgct
     8161 aaattgaggt ttaccaccat tgtttagccc ggaaaggttt aaatttttcc aaacagggtt
     8221 tacctctgtg ttggcttcaa catcgcttac gttaataaaa aagatacgtc cttgcgaggc
     8281 ggttaaaact attttatcag aggcatgatt agtttcaatg cctaatatgg gatagttgca
     8341 atcgtctatt gttatagttc gggttccggc aggttgggca taaacagttg ttacaaaagc
     8401 aagactgagg atagctaata gaatgggctt cataagttaa ttgtaaagtt ctttttcata
     8461 tttgtattgt atgcagttga caccactttc aatgatatgt taatacgata aacagaaatt
     8521 aatgttacaa agtgtttttg aatgccatct gtcgtgagat actttctaaa gaaaaaagaa
     8581 cctcgcaaag actgcaaaga atgtaacacg caaagactgc aaagaatgta acacgcaaag
     8641 actgcaaaga atgtaacacg cagagaccac taagaatgta acacgcaaag accgcaaaga
     8701 atataacacg caaagagcgc aaagaaaaag ttgaactcat ggtattctat gtaaatctta
     8761 ttttatagtt ctactaaaag gatagccttt ctgaactaaa aaatattttc aaaaacaatt
     8821 caacaacttt caaactttgc aactttctaa actttccaaa ctcaaaggct taacgataac
     8881 tattcctgaa cataatttta ataaacgttc tttcaagtat ggcctgtgct agtgcttgct
     8941 gattatcctg tttatttaaa actttcttta atgttttctc aggaatgtca actctataaa
     9001 gccatgcatt taaagttgaa gagcctgatt ttgccaactg acggataatt acttctaatt
     9061 gcattataat atcctgataa gctgaaaaag tatcgccact ccatgcaaca gtaaatccac
     9121 aaggaattaa atctttgttt aattgcacaa ctgtttgttc aattaagtta cgttgctgaa
     9181 aattctgatt agaaagttgt tccatttcca tcctgctctt cttaagtcag gagtttagca
     9241 atgcaagcag tgcaagacgg taggaattta atccaaatcc tgtgattaca cctttacaat
     9301 tggcagcaag cagactttta tgtctgtact cttcgcgtga atgaatattg gaaatatgca
     9361 cttcaaccac cggtgacggt attgccgcaa tcgcatctgc tatggcaaca cttgtatgtg
     9421 tataacctcc tgcattgatg atgataccat cggctttaaa tccttcgtta tgcaaaaggt
     9481 taataatttc accttcaaca ttactctgaa atatttgaat attgggttca ggaaattctt
     9541 tttttaatgt tattgcaaaa tcatcaaatg tctgatgacc ataaatttcg ggctcacgtt
     9601 tgccaagaag attcaggtta gggccgttga tgattacaat tctttttgcc ataaaaaatt
     9661 atgggatgtt taggtactta tgcgtttgta gtgaaattga ccactgagga tttctcttta
     9721 tgtaatcaat cattatcgga ataactctat caaacctact gtattcaggt tgtagaaaaa
     9781 gttttgcaac atcagaggct ttacgtgcat tttcttcagc ccatttaaaa tccgac
//
LOCUS       DAORQT010000041         9640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_934_length_9640_cov_9.745018, whole genome shotgun sequence.
ACCESSION   DAORQT010000041 DAORQT010000000
VERSION     DAORQT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_934_length_9640_cov_9.745018"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK134_02715"
     CDS             <1..671
                     /locus_tag="PK134_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37795.1"
                     /translation="KEFGKDAMEKFLKYELDGYLSGRSSERINEQPIARNDGQGYIHY
                     QKGSLVMYAMKDYLGEDTLNAALHRFIKTYGFQSPPYVQAPQFVQYIADATPDSLKQV
                     VEDMFNRITLFDLKCVEAGYKKKGNKYEVTLDVETGKVYADSMGNGTGVKPNDWIDIG
                     IMKYDGNGKHSYQYLQKQLFTGGRKTIVVTVDEKPSKAGIDPLHKLIDRNSEDNIKEV
                     KSLQ"
     gene            711..2300
                     /locus_tag="PK134_02720"
     CDS             711..2300
                     /locus_tag="PK134_02720"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37796.1"
                     /translation="MLEVKNLKVVFHQNSNPVVAVNDFSFSIAAGETLGLVGESGSGK
                     TVSSLALLGLLNNKNVAISGNASFSLQQPVDLLTLNEKQLTAIRGRHIAMIMQEPMQA
                     FNPLFTCGHQVTEAIRKHTALNKNDATEKVLQQFEKVKLPNPLQISKRFPHQLSGGQL
                     QRVAIAMAISCNPSLLIADEPTTALDATVQKDILLLLQQLQSENNMSMLFISHDLEAV
                     KLIAHNTLVVHAGQMVEQEKTEKIFAHPAQEYTKELLQSRPSGFKRNSVISEKNILSV
                     ENISKSYNQRQGLLRSKQNIIKAVDNISFTVKQDETLGIAGESGSGKTTLAKLLLRLI
                     ETDSGVIKLSEENLKDYPIRTKQLWRKKIQAVFQNPHGSLDPEMTVGRQLTEVFKLHF
                     SSQNTTTVKNQIIEWFEKVGLTEQHFNRYPHELSSGQKQRLCIARALIPQPDLLICDE
                     AVSALDVSVQAKILELINRLQQQFHFGCIFISHDLKVIRQMCDNVLVMKDGKADCYSA
                     TEKLFEKPPTTYSEILLKSII"
     gene            2304..2750
                     /locus_tag="PK134_02725"
     CDS             2304..2750
                     /locus_tag="PK134_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB37797.1"
                     /translation="MINIRKGTPADVEPALRLIKELAEYEKAPEQVINTVEQMLKDGF
                     GEKPIFDLLVAETNDNIVGIAIYFIKYSTWKGKCLYLDDIVVQESLRGKGIGRKLFDA
                     VAAEAKLQNCQQLQWQVLNWNEPAINFYKKYDTVFDDEWINCKIIL"
     gene            2905..3600
                     /locus_tag="PK134_02730"
     CDS             2905..3600
                     /locus_tag="PK134_02730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRB37798.1"
                     /translation="MHPEVRKIFKSITVPLLLSLLMIGVKIFEVLSHTDLGEYGVLPR
                     QISGLKGILFIPFLHADWEHLFSNVVPLLVTGSMLFYFYKEMAVKVILLIYILSGFWL
                     WLGGRENYHIGASNIVYGITAFLFLSGFIRKHTGLIAVSLLMVFLYGSLIWGLFPVVP
                     KISWEGHLFGALSGILCAIVFRKEGQQKEVYEWNDDEPFDDQENDDNATQSSLKIVYS
                     YKKSDEKNGTEES"
     gene            3587..3991
                     /gene="mce"
                     /locus_tag="PK134_02735"
     CDS             3587..3991
                     /gene="mce"
                     /locus_tag="PK134_02735"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRB37799.1"
                     /translation="MKNLEHIGIAVKSLQISDALFEALIGCPPYKKESVESENVITSF
                     FQTGETKVELLEATSPESAIAKFIDKRGEGIHHIAFAVDDIKAEMQRLQQSGFTLLNS
                     EPKKGADNKLVCFLHPKSTNGVLVELCQEIKS"
     gene            complement(3988..4614)
                     /locus_tag="PK134_02740"
     CDS             complement(3988..4614)
                     /locus_tag="PK134_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRB37800.1"
                     /translation="MIKNIIFDFGGVLLPINTEKTVLEFGKMGLDNFDELYSMQKQAK
                     FFDDFEKGLIPASAFRSEIRKHYKRPLSDEEIDYAWNALLGELNPERFNFLSELRDKY
                     NIYLLSNTNIIHHAAFIKSLNQTFGKDRFEKLFEKTYYSFQMGTRKPDKRIFADVIKD
                     SGVTPAETVFIDDNLNNALGAQKVGMNAIHHDNAMEIEKELMAMLSAF"
     gene            4695..4768
                     /locus_tag="PK134_02745"
     tRNA            4695..4768
                     /locus_tag="PK134_02745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4729..4731,aa:Ile,seq:cat)
     gene            4868..6805
                     /locus_tag="PK134_02750"
     CDS             4868..6805
                     /locus_tag="PK134_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRB37801.1"
                     /translation="MVARHDPLNKITLGGLLVTLGIIYGDIGTSPLYVMNAIIGDRPI
                     SPELVLGGISCIFWTLTLQTTFKYVFLTLRADNKGEGGIFSLYALVRKTKVKWLAIAA
                     ILGGSALLADGLITPAISVSSAVEGIQILHPDLPTIPIIITIIFGLFFIQQFGSNFVG
                     KSFGPMMLIWFSMLFVLGFHQLIQNPSVLKAFNPYYAYQLLVNYPGGFWVLGAVFLCT
                     TGAEALYSDLGHVGRKNIYITWTMVKFCLLINYFGQGAHLLKFEGKTIDVNPFYQLMP
                     DWFLLIGIIISTLAAIVASQALISGAFTVVNEAMRLNFGPKLKVVYPTDLRGQVYIPT
                     VNWTLCAGCIGVILFFQHSSNMEGAYGLAIIIDMMITTILLSFYLYLKRYPLPLVMLT
                     FLLFIVIEIAFLVANSIKFEHGGYVTMLLTMFLFITMYVWYAARKIRNQFVDFVKVKD
                     FLEILTNLSKDESIPKYATNLVYLTSANRRDEIESKVMYSLLQKFPKRADIYWFVHVD
                     VLDEPYTMEYKVAEMVNDKVIHIDFRLGFRIEPRINQMFRQVVEELVKNNEVDITSRY
                     TSLKEKNVIGDFRFVVIEKTLSYDNKLNFYEKFVLNAYDFLKLFSLPESKAFGLDTSS
                     VTVENVPLVVKPSGKFKLKRI"
     gene            complement(6802..8007)
                     /gene="hflX"
                     /locus_tag="PK134_02755"
     CDS             complement(6802..8007)
                     /gene="hflX"
                     /locus_tag="PK134_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRB37802.1"
                     /translation="MKEKTVFKPLTDVKERAVLVGVILKDQPVEQEMEYLDELAFLAE
                     TAGAQTLKSFTQKLDHTDPRTFVGSGKLAEINEFIKANEINLAVFDDELSPSQQRNIE
                     KILQIKVLDRTQLILDIFARRARTAQAQVQVELAQYQYLMPRLTRMWTHLEKQQGGIG
                     NRGPGEKEIETDRRIVRDRLSKLRERLKEIEKQGFNQRKARGNMVRVALVGYTNVGKS
                     TIMNLLSKSDVFAENKLFATLDTTVRKVVLENIPFLLSDTVGFIRKLPHHLVESFKST
                     LDEVREADILIHVVDISHPNFEAQINVVNQMLQELQSADKPVILVFNKIDAFTYIEKE
                     ADDLTPMTSENISLDDLRKTWMNKGNVETIFISATEKDNISELRTMLLEKVKRIHLVR
                     YPNNKITND"
     gene            complement(8030..8650)
                     /locus_tag="PK134_02760"
     CDS             complement(8030..8650)
                     /locus_tag="PK134_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HRB37803.1"
                     /translation="MYTGILHTHTLVVSLFIVLYLIKATLLVLNRHAALDNFVKKTKV
                     AEMIISTLFLLTGIYLAVNTGNKGTWLWVKLFVIACTIPLAVIAFKKKNSSLALISLM
                     ALIYAYGISETKSISMKKADTAVNTEGLEGAAAGKVIFESKCQSCHGADGKLGLSGAK
                     DLTQSALSPQEKIDIITNGKKTMAAYKDQLSQEQIAAVAEYVQTLK"
     gene            complement(8675..8762)
                     /locus_tag="PK134_02765"
     tRNA            complement(8675..8762)
                     /locus_tag="PK134_02765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8726..8728),aa:Ser,seq:gga)
     gene            complement(8954..>9640)
                     /locus_tag="PK134_02770"
     CDS             complement(8954..>9640)
                     /locus_tag="PK134_02770"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRB37804.1"
                     /translation="VILTGSQLPIGEIRTDAKSNLITAAEIACGNVVVPEVCICFNNQ
                     LFRGNRAEKFTSSKFDAFQSINYPSLAEAGTEINYNHDAIREVNNNALKVKTHFNNSV
                     MIFKLFPGVNEKVLSAILKSGALKGLIIETYGSGNAPKNEKLLKQLGDAVKNQMVIVN
                     VSQCSGGVVQMEKYENGLALKRAGVVSGHDMTTEAAITKLMFLLGSEMDYQEIKVNMQ
                     LDMAGELSRE"
BASE COUNT         3009 a         1800 c         1773 g         3058 t
ORIGIN      
        1 aaaaagagtt tggaaaagat gccatggaaa aatttcttaa atacgaattg gatggctatt
       61 tatcaggacg ttcatcagaa agaataaatg agcaacctat tgcacgcaac gatggacagg
      121 gatatattca ctatcaaaaa ggtagtttgg taatgtatgc aatgaaagac tatttaggcg
      181 aagacacact aaatgcagcc ttacacagat ttataaaaac gtatggattt cagtccccac
      241 cttatgtaca agcaccgcag tttgttcaat acattgcaga tgcaacacct gacagtttaa
      301 agcaagtggt tgaagatatg tttaaccgta tcacgctgtt tgatttaaaa tgtgttgaag
      361 ccggctacaa aaagaaaggc aacaaatatg aggttacctt agatgtagag accggaaaag
      421 tttatgcaga cagtatggga aatggaacag gggttaaacc aaatgactgg attgatatag
      481 gcataatgaa atatgatggc aacggaaaac actcttatca atatttgcag aaacaattat
      541 ttacaggtgg cagaaaaaca attgttgtta ctgttgatga aaaaccgtct aaggcaggta
      601 ttgacccatt gcataaactg attgacagga atagcgaaga taacatcaag gaagtaaaat
      661 ccttacaata atttttctga cattgacatt tactttttat cttggcagcc atgcttgagg
      721 taaaaaatct gaaagttgta tttcatcaaa acagtaatcc tgttgttgct gtaaatgatt
      781 tttctttttc tattgcagca ggcgaaacct taggattggt tggcgaatca ggttccggga
      841 aaactgtttc gtcattggca ttacttggat tgttgaacaa taaaaatgtt gctataagtg
      901 gcaatgcatc tttcagttta caacaacctg ttgatctttt aacgctaaac gaaaaacaat
      961 taacagctat cagaggtcgt catattgcaa tgataatgca agagcctatg caggcattta
     1021 atcctctgtt tacttgtggg catcaggtga ctgaagctat tcgaaaacac actgcattaa
     1081 acaaaaatga tgcaacagaa aaagtattgc aacagttcga aaaagtaaag ctaccaaatc
     1141 cattacaaat aagtaagcgt tttcctcatc aactttcagg agggcagcta cagagagttg
     1201 ccattgcaat ggctataagt tgcaatccat cacttctaat tgctgatgaa ccaacaacag
     1261 ccttagacgc cactgtgcaa aaagacattc ttttattgct acaacaatta cagtcagaaa
     1321 ataacatgag catgcttttt atcagtcacg atcttgaagc agtaaaattg attgcacata
     1381 acacattagt ggttcatgca ggacagatgg ttgagcagga aaagacagaa aaaatatttg
     1441 cacatcctgc acaagaatat acaaaagagc tattgcaaag ccgtccttca ggattcaaaa
     1501 gaaacagtgt tatcagtgaa aagaatattc tgtcagtaga aaacatcagc aaaagttaca
     1561 accaacggca agggctatta cgcagtaaac aaaatattat aaaagctgtt gacaatatca
     1621 gttttacagt aaagcaagat gagactttag gcattgccgg tgaatcggga tctggaaaaa
     1681 caacattagc taaattatta ctgcgactta ttgaaactga tagtggcgtg ataaaattaa
     1741 gcgaagaaaa tcttaaagac taccctattc gcaccaaaca attatggcga aaaaaaattc
     1801 aggcagtatt tcaaaatcca catggctcac tcgatccgga gatgactgtg ggcagacaac
     1861 ttacagaagt ctttaaatta catttttcat cacaaaatac cactactgtt aaaaaccaga
     1921 ttattgaatg gtttgaaaaa gtaggattaa cagaacaaca ttttaaccgc tatccacatg
     1981 aattaagttc cggacaaaag caaagactgt gcattgcccg tgctctgata ccacaacccg
     2041 atttacttat ctgcgatgaa gctgtgagtg cacttgatgt ttctgttcag gcaaaaattc
     2101 ttgaattgat aaacaggctg caacagcaat ttcatttcgg atgtattttt atttcgcatg
     2161 acttgaaagt tatccgtcag atgtgtgaca atgttttggt gatgaaagat ggcaaagccg
     2221 actgttattc agcaactgaa aaactatttg agaaacctcc tacaacctat tcagagatat
     2281 tattaaaatc tattatttaa aaaatgatta acatacgcaa aggaacacct gcagatgttg
     2341 aacctgcact tcgtcttatc aaagaattgg cagaatatga aaaagcgcca gaacaggtta
     2401 tcaatactgt tgagcaaatg cttaaagatg gctttggaga aaaaccgatt tttgatttgt
     2461 tagttgcaga aaccaatgac aatatagtgg gtatagcaat ttactttatc aaatattcaa
     2521 catggaaagg aaagtgtctt tatttagatg atattgttgt gcaagaatca ttacggggaa
     2581 aaggtatagg ccggaaattg tttgatgctg ttgcagcaga agccaaatta caaaactgcc
     2641 aacagttgca atggcaggta ctgaactgga acgagcctgc aattaatttt tataaaaagt
     2701 acgatactgt ttttgatgat gaatggatca attgtaaaat tattctgtag tctttctttg
     2761 ttacattcgt acagacaaat tcaattgcac aaaacacatt gtcattaaat cagtatttca
     2821 ctgccaaacc aggaaaagca tcagcaataa aggcagattg tttttgttta accacaatgg
     2881 cagattgttt gtaaggtaaa acacatgcat ccggaagtcc gtaaaatttt taaaagcatt
     2941 acagtaccac tcctcctatc acttctaatg ataggtgtaa aaatttttga agtcttatcg
     3001 catactgatt taggtgagta cggtgttttg ccaagacaaa taagcggatt aaaaggcatt
     3061 ttgtttattc catttcttca tgccgactgg gaacatttgt tcagtaatgt tgttccgctg
     3121 ttagttacag gcagtatgtt attttacttt tataaagaaa tggctgtaaa ggttatactg
     3181 ctcatttaca ttttaagtgg cttttggctt tggcttggtg gacgagaaaa ttatcatatt
     3241 ggtgccagca atattgttta tggcatcaca gcatttttat ttctaagtgg ttttatcaga
     3301 aagcatacag ggcttattgc tgtttcattg ctcatggttt ttttatacgg cagtttaatt
     3361 tgggggcttt ttcctgtggt tccaaaaatt tcatgggaag ggcatttatt tggtgccctg
     3421 tccggaatac tttgcgcaat agttttcaga aaagaaggtc agcagaagga agtttatgag
     3481 tggaacgatg atgaaccctt tgatgaccaa gaaaatgatg ataatgcaac acagtcatca
     3541 ttaaaaattg tttattcgta taaaaaatca gacgaaaaga atggcactga agaatcttga
     3601 acacattggt attgcagtta aaagtctgca aatttctgat gctttatttg aagcattaat
     3661 aggttgtccg ccttataaaa aagaaagcgt tgaaagcgaa aatgtaatta cctcattctt
     3721 tcagacgggc gaaacaaaag ttgaactttt agaagcaact tcaccggaaa gtgccattgc
     3781 aaaatttatt gataaacgtg gtgaaggaat tcaccacata gcctttgctg ttgatgatat
     3841 taaagcagaa atgcaacgac tgcaacaatc aggctttaca cttttaaaca gtgagcccaa
     3901 gaaaggtgcc gacaataaat tggtttgttt ccttcaccct aaatcaacta atggtgtgtt
     3961 ggtagaattg tgtcaggaaa ttaagtctta aaaagcagag agcattgcca tcagttcttt
     4021 ttcaatttcc atggcattgt catgatgaat ggcattcata cctacctttt gtgcacctaa
     4081 tgcattgttc agattatcat caataaaaac ggtttcagca ggagttacac cactgtcttt
     4141 tatcacatca gcaaaaattc ttttatcagg cttgcgtgtt cccatctgaa aagaataata
     4201 tgttttttcg aacagtttct caaagcggtc ttttccaaaa gtctgattaa gactcttgat
     4261 aaatgcagca tgatgaataa tgttggtatt actcaaaagg taaatattat acttatcacg
     4321 aagctcactc agaaaattaa atctctcggg attcagttca ccaagcaagg cattccatgc
     4381 ataatcaatt tcttcatcag aaagcggacg tttataatgc tttcttattt cgctgcgaaa
     4441 tgcagaggca gggataagtc ccttttcgaa atcatcaaaa aacttggctt gcttttgcat
     4501 tgaatacagt tcgtcaaaat tgtccaaccc catttttcca aactccaaaa cggttttttc
     4561 tgtatttatc ggaagtaaca ccccaccaaa atcaaaaatg atatttttaa tcattctctt
     4621 gctatctttt gtttattgcg tgcaaaaata tacttttgca gccctcaaag ttcatttaac
     4681 ttataattct tttcgggccc atagctcagt cggttagagc aacagactca taatctgtgg
     4741 gtccctggtt cgagcccagg tgggcccact ttctttagct cgataaaaac cacactttat
     4801 taaaaattct tttttccttt ctgaagttaa gtttcaagaa taataccttt gtcatcattt
     4861 ttataatatg gttgcacgcc acgatccgtt aaacaaaata acacttggag gacttttagt
     4921 caccttggga attatctatg gcgacatagg tacatctcct ctctatgtaa tgaatgccat
     4981 cattggcgac agacccataa gtcctgaatt agtacttgga ggtatcagtt gtatattctg
     5041 gacattgaca cttcaaacaa cttttaaata tgttttcctg acactgcgtg ccgacaacaa
     5101 aggtgaaggt ggaatttttt cactctatgc attagttaga aaaactaagg taaagtggtt
     5161 ggctattgct gcaatacttg gaggcagcgc cttacttgct gatggattaa tcacccctgc
     5221 catctctgtt tcgtctgctg tagaaggtat tcagattctt catccagacc tacctacaat
     5281 acctattatt attaccataa tctttggttt gttttttatt caacagtttg gcagcaactt
     5341 tgttggaaaa tcttttggtc ctatgatgct gatttggttt tccatgttat ttgttttagg
     5401 atttcatcaa ctcattcaga acccgtctgt acttaaggct ttcaaccctt actatgccta
     5461 tcagttactt gtaaattatc ctggaggctt ttgggtcctt ggtgcagttt ttctttgtac
     5521 tacaggagca gaagcacttt actctgacct tggtcacgta ggacgtaaaa atatttatat
     5581 tacctggaca atggtgaaat tttgcttgtt gatcaattat tttggtcagg gtgcacacct
     5641 gctaaagttt gaaggaaaga caattgatgt aaatccattt taccaactta tgcctgattg
     5701 gtttctgtta ataggcatca ttatttctac tcttgcagca attgttgcca gccaggcatt
     5761 aatcagtggt gcatttaccg tagtaaacga agccatgcga ctgaactttg gcccaaaact
     5821 taaagttgtt tatccaacag acctcagagg gcaagtatat attccaaccg ttaactggac
     5881 tttatgtgca ggatgtattg gcgtaatact attttttcaa cactcatcca atatggaagg
     5941 tgcttatgga ttagcgataa tcattgatat gatgattaca acaattctgc tttcattcta
     6001 tttgtattta aaacgttatc cgctaccgtt agtaatgctt acatttcttc tttttattgt
     6061 cattgaaatt gcttttctgg tggccaactc cattaaattt gaacatggcg gatatgttac
     6121 catgctactt actatgtttt tattcatcac catgtatgtt tggtatgcag cacgtaaaat
     6181 cagaaatcag tttgttgact ttgttaaggt aaaagatttt ttagagatac ttaccaacct
     6241 gagtaaagac gaatctattc ctaaatatgc taccaacctt gtgtatctta ccagtgccaa
     6301 ccgcagagat gaaattgaat cgaaagtgat gtattcgctc ttacagaaat ttccgaagcg
     6361 tgccgacatc tattggtttg ttcacgttga tgtgcttgat gaaccttaca ccatggaata
     6421 taaggttgca gaaatggtaa atgataaggt tatccatatt gatttcagac ttggtttcag
     6481 aatagagcca cgaatcaatc agatgttccg tcaggttgtt gaagagcttg taaaaaataa
     6541 tgaggttgat attaccagtc gctacacttc acttaaagaa aaaaatgtga taggtgattt
     6601 ccgttttgta gtgattgaga aaacactatc gtatgacaac aaactgaatt tctatgaaaa
     6661 gtttgtgctc aatgcttatg actttttaaa attatttagt ttgcccgagt caaaagcctt
     6721 tggtcttgac accagttctg taactgtaga aaacgttcct ttagtagtaa aaccttcagg
     6781 taaatttaaa ctcaaacgaa tttaatcgtt tgtaatttta ttgttgggat aacgcaccaa
     6841 gtgaatacgt tttacttttt cgagtaacat ggttcgcagt tcagaaatat tatctttctc
     6901 tgtagctgaa ataaatattg tttctacatt gcctttattc atccacgtct ttcgtaaatc
     6961 atctaatgaa atattttcgc ttgtcatggg tgtcaggtca tctgcttctt tttcaatata
     7021 agtaaatgca tcaattttat taaagacaag aattacaggt ttgtctgctg attgaagttc
     7081 ctgcaacatc tgattcacca catttatctg tgcttcaaaa ttgggatgcg aaatatccac
     7141 tacatgaatt aaaatatcgg cttcgcgcac ctcgtcaagt gttgacttga atgattcaac
     7201 taaatgatgt ggcagctttc tgataaatcc aacagtatcg ctcaacaaaa aaggaatatt
     7261 ttcaagcaca acttttctga ctgtagtatc taatgttgca aagagtttgt tttctgcaaa
     7321 cacatctgac ttgctaagca gattcataat tgtggacttg cccacattag tataacctac
     7381 caatgcaaca cgcaccatat tacctcttgc cttacgttga ttaaagcctt gcttctctat
     7441 ttcttttaaa cgttcacgga gtttactaag ccggtcacga acaatacgtc tgtctgtttc
     7501 aatttctttt tcaccgggac cgcggttacc tatacctccc tgctgttttt cgaggtgtgt
     7561 ccacatgcgt gtcaggcgtg gcatcagata ttggtattgt gcgagctcaa cctgcacctg
     7621 tgcctgcgct gtgcgtgccc ttcgtgcaaa aatatcgagg ataagttgtg ttctgtccaa
     7681 tacctttatc tgtaatattt tttcaatgtt gcgttgctgc gaaggcgaca actcatcatc
     7741 aaaaacagca aggtttattt cattcgcttt gataaactca tttatctctg ctagttttcc
     7801 tgaacctaca aaggtgcgtg ggtcggtatg atctaacttt tgtgtaaacg attttaaggt
     7861 ctgtgcgcct gcagtctctg caagaaaagc caactcatca agatactcca tctcctgttc
     7921 tacaggctga tcttttaata ttacgcctac taaaacggca cgctccttaa catcagtaag
     7981 tggcttgaaa acggtttttt ctttcatcaa taaataaaac tgaaataaat tatttcagtg
     8041 tctgaacata ttctgcaacg gcagcaattt gttcttgtga taattgatct ttataggctg
     8101 ccattgtttt tttgccattg gtaatgatgt caattttttc ctgtggagat aaagcagact
     8161 gagtaaggtc ttttgcacca ctcaatccta attttccatc agcaccatga caagactggc
     8221 atttactttc aaaaataacc ttacctgcag cagcaccttc aaggccttct gtatttacag
     8281 ctgtatcagc ctttttcatg gatattgatt tggtttcaga aatgccataa gcataaatca
     8341 atgccatgag tgaaatcaat gccaatgagc tgtttttctt tttaaatgca attactgcaa
     8401 gtggaatagt gcaggcaatg acaaacaatt taacccacaa ccatgtaccc ttgttacctg
     8461 tgttgacagc cagataaatt ccggttagta ggaacaatgt gctgatgatc atttctgcaa
     8521 ccttagtttt tttaacaaag ttgtctaatg cagcatgcct gttcagcact aacaaggttg
     8581 ctttaatgag gtataaaaca ataaaaagtg aaactaccag tgtgtgtgtg tgtaaaattc
     8641 ctgtgtacat aatgataaag ataaatgatt tttgcggaga gagagggatt cgaaccctcg
     8701 ttacactttt ggcgtaaaca cactttccag gcgtgctcct tcaaccactc ggacacctct
     8761 cctttttgag cctgcaaagc taacaaatta acatgatggc taaagaaaaa gcctgccgaa
     8821 aaaaatacga caggctgaga acctaaaacc aaacctatct tttgaccgtt gtcaacagat
     8881 tgattaaaac cgcatcaaaa gtaggttgac aaattgaaca actgcgccaa tgcgtagttt
     8941 gttatcaaca catttattca cgactaagtt ctcctgccat atccaattgc atattcactt
     9001 ttatctcttg ataatccatt tcgctgccca ataaaaacat aagtttggta atggcagctt
     9061 cagtagtcat gtcatggcca ctgactacac ctgcacgttt taacgcaagt ccattctcat
     9121 acttttccat ctgcacaacg cctccactac actgcgaaac atttacaatt accatttgat
     9181 tttttactgc atcgcctaat tgctttagca gtttttcatt cttaggcgca ttgccggagc
     9241 catacgtttc aatgatcaat ccttttagcg cacctgactt taaaattgct gacaatactt
     9301 tttcatttac tcccggaaac aatttaaaaa tcatcaccga attattgaag tgcgtcttta
     9361 ctttcagcgc attgttgtta acctcacgaa tggcatcgtg gttgtaatta atttcagtgc
     9421 ctgcctcagc caatgacgga tagttgatgg attgaaaagc atcgaatttt gaagatgtaa
     9481 atttttctgc tctgttacct ctgaacaact gattgttaaa acaaatacaa acctccggca
     9541 ccacaacatt tccacaggca atttcagctg ctgtaataag gtttgatttt gcatctgttc
     9601 tgatttcgcc aataggtaat tgtgatccgg tgaggattac
//
LOCUS       DAORQT010000042         9466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_964_length_9466_cov_10.103177, whole genome shotgun sequence.
ACCESSION   DAORQT010000042 DAORQT010000000
VERSION     DAORQT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_9466_cov_10.103177"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1510
                     /locus_tag="PK134_02775"
     CDS             <1..>1510
                     /locus_tag="PK134_02775"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB37805.1"
                     /translation="VTHTYNTPGIYYVRLVVTNSAGCKDTSFIIPIYVGSPVTTSFTV
                     VPTTICAYTPVQFNVLNPDTNNIYHFSGDGGMLSHCYNQTNPIWSFNTQTGQQTITLS
                     TSNNGCISTATQTVTVKGPMAKFYVAGNCNTPFKYSFPATINDATTWNWDFGDGTALI
                     NSGNVNPTHTYAATGDYWVKLTSFNTTSGCAPDIDSNIVRVRNISAEFTRDSVACKSY
                     TINVDATNATDAYGSCNEGFLWYWGDGTFPHNSSSPIAAHTYFNSGVFYVKLVTTDIN
                     GCVDSVKKKVKVYESNAFFKTNKNYGCLPLTVNFTDSSYADTTIASYSWDFGDGSTSS
                     VKNPSHTYTTAPAGGSWTVTLIVTDTIGCTDVYQKTITASLPDTLFSATKTNICVGEP
                     IVFNPHFTGHSSYSWSFGDNTTSAQVSPSHSYNAAGAYTVTLSVTDSIGCPGSKKITN
                     YINVQAYPKAGFTSSADSMLNKCYPLLVNFYDTSIVSVFGSRKWNLGNSSNIF"
     assembly_gap    1511..1520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1521..2872
                     /locus_tag="PK134_02780"
     CDS             <1521..2872
                     /locus_tag="PK134_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB37806.1"
                     /translation="AGTIYQTPGIYYIQLIEETTFGCRDTAYDTLRVLGPVGNFNIAP
                     TTICKGQSITFSIKDTADLGAFSWDFGDGMTSPGISPITHTYNINPVSGQTLVGLVMW
                     SPDSACTYTQTNPVFIRKVIANFNINSGDTALCIGEPVTLVNTSLNASNNYWNFGNGQ
                     TFNGATPPPYTINQTGVYTITLAIDNSAWGCKDTIRKKISINNLPTVNAIGGDTCAGK
                     PITLHATGGVSYLWSPATGLSNTTVANPVATPGQSTVYTVLITDVNGCTGVATAPVTI
                     YNPPPQVNVTHLLFVGDSVQLNTGLTNNLIINWNPINGLSCVDCPNPWAQPLVNTLYT
                     ATFTDSAGCFSGISRFNIEIDPRITIDVPTAFTPNGDGDNDVIFVNGLGLKRLIEFKI
                     YNRWGQLIFESNDLNKGWDGYFKGQLQNVETYVYTAKAEGWLNGKIVSKKGTFNLLR"
     gene            2921..3160
                     /locus_tag="PK134_02785"
                     /pseudo
     CDS             2921..3160
                     /locus_tag="PK134_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            3170..4036
                     /locus_tag="PK134_02790"
     CDS             3170..4036
                     /locus_tag="PK134_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB37807.1"
                     /translation="MKISIRFFNDREVRAVWDDANAKWWFSVLDIVGVLNNESDYSKV
                     RNYWKYLKAKLKKENNQLVSAATQFKFLAPDGKKRMADMLDSDGVTALAKNFPNNRAM
                     KFLDWFLYSDTSIDGQSKKKAYTLFESNLINEFEVGTTKGLQQIHAYLFGGLYDFAGQ
                     IREKSISKGGYQFVYAQHLKATLQQIDAMPQKALDEIIIKYTEMNKAHLFMEGNGRST
                     RIWLDLMLKKQLKKCVDWSKISKENYMNAMIISTVDSSVLIQVIKPALTNKINDREMF
                     MKGIDYSYYYEE"
     gene            4122..4412
                     /locus_tag="PK134_02795"
                     /pseudo
     CDS             4122..4412
                     /locus_tag="PK134_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(4988..6010)
                     /gene="pheS"
                     /locus_tag="PK134_02800"
     CDS             complement(4988..6010)
                     /gene="pheS"
                     /locus_tag="PK134_02800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRB37808.1"
                     /translation="MSQELIEQHIQEAETFSIQSPADLENFRLKYLSKKGVLADMFAL
                     MKNIEPAERKNYGLQVNLLKQKTEAIFESFKEKFENVTTDEKKIDITLPGEPMSEGAL
                     HPLSIIRNRIIEIFSAVGFTVSDGPEIEDDWHNFTALNFPEEHPARDMQDTFFIRKNP
                     DLALRTHTSSVQVRIMEKHKPPLRTISPGRVYRNEAISARAHCFFHQVEGLYIDKDVS
                     FADLKQTLLYFAREMFGADTEIRLRPSYFPFTEPSAEMDISCNFCKGEGCNICKYTGW
                     VEILGCGMIDPAVLDNCKIDSNVYSGFAFGMGIERITMLVYNINDLRIFSENDVRFLK
                     QFSGIG"
     gene            complement(6103..9414)
                     /gene="secA"
                     /locus_tag="PK134_02805"
     CDS             complement(6103..9414)
                     /gene="secA"
                     /locus_tag="PK134_02805"
                     /inference="COORDINATES: protein motif:HMM:NF009536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRB37809.1"
                     /translation="MIDFINKTISKIFGSKAESDYKALSPVIEAVNAVAENMSSLSND
                     ELRNKTDEFRTRIQDYLSEIDTELTQLNEEAEADPEMDANQKEEIYNRIDALKKERNK
                     KTEEVLNEIQPEAFALVREVAKRFTENAELAVEANDNDKNLVTKRDGIRIEDGKAIYS
                     NTWTAAGSKVLWNMIHYDVQLIGGSVLHQGKIAEMATGEGKTLVATLPIYLNALAGAG
                     VHVVTVNDYLAKRDAEWNGPLFEFLGLSVDCIDKHQPNTEERRKAYLADITYGTNNEF
                     GFDYLRDNMTRHAHEMVQRPHHFAIVDEVDSVLVDDARTPLIISGPTPKGDTQEYFAL
                     KPKVEILFNAQRNYINTVIADAKKKLGENKPDEAGMPLIRAYRGLPKNRALVKFLSEQ
                     GVRALLQKTENHYMQDQSKEMHKVDAELFFVIDEKNNTVELTEKGIELITANNEDTHF
                     FVMPDVGASIAEIEKSNGTNEEKLERKEALMRDFSVKSERIHTINQLLKAYTLFEKDD
                     EYIIADGKVKIVDEQTGRVLEGRRYSDGLHQAIEAKEGVKIEAATQTYATVTLQNYFR
                     MYHKLAGMTGTAETEAGEFWQIYKLDVVVIPTNKKIIRDDREDLVYKTKREKYNAVIE
                     EIAKLTAAGRPVLVGTTSVEISELLSRMLKQRNIKHNVLNAKLHKKEADIVAEAGQAG
                     TVTIATNMAGRGTDIKLGQGVKEAGGLAIIGTERHDSRRVDRQLRGRSGRQGDPGSSQ
                     FFVSLEDDLMRLFGSERIAKLMDRMGIEEGEVIQHSMITKSIERAQKKVEENNFGIRK
                     RLLEYDDVMNAQREVIYKKRRHALFGERLALDIMNMQYDVCESIISSYNQERDFDALQ
                     LDLIRYLSIEAPIDAKAFLQIKLEDLTQMVFEKANGHYRQKSNLIAEKAFPVIENIYT
                     NQGQTIVDVVVPFSDGTKAMNVIAGLKKSYDSKCKELISALEKSSVLNFIDEAWTEHL
                     REMDDLRQSVQNAVYEQKDPLLIYKFESFELFKQMIDRVNKEIVSFLIKSYIPVQNQE
                     NVREAKVQQQRTDMSKMQTSHNESDAGGQSRPQGPPPPPKQQPAKAEIKVGRNDPCPC
                     GSGKKFKNCHGAGVD"
BASE COUNT         2891 a         1929 c         1752 g         2884 t
ORIGIN      
        1 gttacacaca cctacaacac accgggtatt tactatgtga ggttagtagt tacaaattct
       61 gcaggatgca aagacacttc ttttataatt ccgatttatg taggttctcc tgttacaaca
      121 tcatttactg ttgttcctac aactatatgt gcatacacac ctgtacagtt taatgttctg
      181 aatcctgaca ccaataatat ttatcatttt tctggtgatg gaggaatgct gtcgcattgt
      241 tacaatcaaa ccaatcctat ctggagtttc aacacacaaa cagggcaaca gacaattaca
      301 ctttctacat caaacaatgg ttgcatcagc acagctacac aaactgtcac tgttaaagga
      361 cctatggcga aattttatgt ggccggaaat tgtaatactc catttaaata tagttttcct
      421 gcaacgatta acgatgctac cacctggaac tgggattttg gtgatggcac agcattaatt
      481 aattcaggaa acgtaaatcc aacacatacc tatgctgcta caggcgacta ttgggtaaaa
      541 ttaacttcat tcaacacaac atctggttgt gcacctgata ttgattccaa tattgttcgc
      601 gtcagaaata tatcggcaga atttaccaga gattctgttg cctgtaaaag ctatactata
      661 aatgtagatg ccacgaacgc tactgatgct tatggcagtt gcaatgaagg ttttttatgg
      721 tattggggcg atggaacctt tcctcataat tcttcttcac caattgctgc ccacacttat
      781 tttaacagcg gtgtgtttta tgtaaaactt gtcaccactg acattaatgg atgcgtggat
      841 tcagtaaaga aaaaagtgaa agtttatgaa tccaatgctt tttttaaaac caataaaaat
      901 tatggctgtc tgccactaac ggtgaatttt actgacagta gttatgccga tacaaccata
      961 gcttcttact catgggactt tggtgatgga agcacttctt ctgtcaaaaa cccaagtcac
     1021 acctacacta ctgcacctgc aggtggaagt tggacagtaa cattaattgt tactgatact
     1081 attggctgca ccgatgttta ccaaaagaca attactgcgt cattgccgga tacgttattc
     1141 tctgcaacaa aaacaaatat ttgcgttggc gaacctattg tcttcaatcc acattttacg
     1201 ggacattcat catactcgtg gagctttggt gacaacacca catcagcaca ggtatcacca
     1261 tcgcatagtt ataatgcagc aggtgcctac acagtaacat tatctgttac cgattccatc
     1321 ggctgtcctg gaagtaaaaa aattactaac tacatcaatg tacaagccta tcccaaagca
     1381 ggatttacca gtagtgcaga cagtatgctc aataaatgtt atccattgtt ggttaacttt
     1441 tatgatacaa gtattgttag tgtttttggc agccgcaagt ggaatcttgg caacagcagt
     1501 aacattttta nnnnnnnnnn tggcaggaac aatttatcag acgcccggca tttactacat
     1561 tcaacttatt gaagagacaa cctttggttg cagagacact gcttatgata cacttcgtgt
     1621 acttggacct gttggcaact tcaacattgc accaacaaca atttgtaaag ggcaatcaat
     1681 tactttcagc attaaagata cagcagacct tggtgcattt tcgtgggact ttggtgatgg
     1741 tatgacctct ccgggaattt cacccataac acatacatac aatattaatc ccgtaagcgg
     1801 acaaacgctt gtaggtttgg taatgtggtc gcctgactcg gcttgtactt acacacaaac
     1861 caatccggtg tttattcgaa aagttattgc aaacttcaac attaacagtg gcgacactgc
     1921 tttatgtatt ggtgaacctg ttacattagt caatacttct ttaaatgcaa gtaataacta
     1981 ctggaacttt ggtaatggtc agacttttaa tggtgcaaca ccaccgcctt acaccattaa
     2041 tcaaacaggg gtttacacca ttacattagc aatagataat tcagcgtggg gatgtaagga
     2101 cactatcaga aaaaaaataa gcataaataa tcttccgaca gtaaatgcaa taggtggcga
     2161 cacttgtgca ggcaaaccaa taactttaca tgcaacaggt ggtgtaagtt atctgtggtc
     2221 gccagcaaca ggactttcaa acactacagt tgccaatcct gttgccacac cgggtcaaag
     2281 caccgtttat accgtattga taaccgatgt aaacggatgt acaggagttg ccactgcacc
     2341 tgtaacaatt tacaatccac ctccgcaagt caatgtaacg catttacttt ttgttggcga
     2401 ctctgttcaa ttaaatacag gccttacaaa taatttaatc atcaattgga atcctattaa
     2461 cggattaagt tgtgttgatt gccctaaccc atgggcacag ccgttggtaa acacacttta
     2521 cactgcaaca tttacagatt ctgcaggttg cttttcaggc atttcaagat tcaacataga
     2581 aatagatcca agaataacta ttgatgttcc aacagctttt acacccaacg gtgatggcga
     2641 taacgatgta atatttgtaa atggactcgg actgaaacgt ctgatagaat ttaaaattta
     2701 caatcgttgg ggacagttga tttttgaaag caacgactta aacaaaggct gggatggcta
     2761 ttttaaaggt cagttgcaga atgtagagac ctatgtttac actgccaaag cagaaggttg
     2821 gttgaatggc aaaattgtga gtaagaaagg aactttcaat ttgttgagat gatgtaaacc
     2881 ataataagat atcacatagg aacaaaattt tagaaaaagc aagagggatt tgtttgttga
     2941 agagaatatt atggtagagt tgaaagccct gataaaactt gaagaagtgc atttggcaca
     3001 tgcaatgaat tattaccagg cctataattt gcccataggc ttacttatca attttggtgc
     3061 aaagagcctt gaatttaaaa gggtgtataa tgttaatcat cctgagaata agggctacat
     3121 caaacgcaat cctacaatca tcaaatccaa caaatcttaa ttcagacaaa tgaaaatatc
     3181 catccgcttc ttcaacgacc gtgaggtgcg ggctgtttgg gatgatgcca acgccaagtg
     3241 gtggttttcg gtattggata ttgtgggtgt attaaacaat gaaagtgatt acagtaaagt
     3301 ccgtaactat tggaaatacc ttaaagccaa gttgaagaaa gaaaacaatc aactggttag
     3361 tgccgctacc cagttcaaat ttcttgcacc cgatggcaaa aagagaatgg ctgatatgtt
     3421 ggatagtgat ggcgtaacag ctctcgccaa aaattttcca aacaacagag ccatgaagtt
     3481 tttagactgg tttctttata gcgataccag cattgacgga caaagcaaga aaaaagccta
     3541 cacccttttt gaaagtaatt taatcaacga atttgaagtg ggaacaacta agggattaca
     3601 acaaatacac gcttacctgt ttggtggctt atatgatttt gctgggcaaa taagagaaaa
     3661 aagtatatcg aaaggaggat atcaatttgt ttatgcacag catttaaaag ccacattgca
     3721 gcaaattgat gccatgccac aaaaagcgct tgatgagatt attattaaat atacagaaat
     3781 gaataaggca catcttttta tggaaggcaa cgggcgaagc acaagaatat ggcttgattt
     3841 gatgcttaaa aaacaactga aaaaatgtgt ggattggagt aaaataagca aagagaatta
     3901 tatgaatgct atgataatta gcacggttga tagcagtgta ttgatacaag tgataaagcc
     3961 cgccctaacc aataaaatca acgacaggga aatgtttatg aaaggtattg attactcata
     4021 ttactatgaa gaatgataaa ggtggtttcg gctgcgctca accaccagct actattccgt
     4081 tgcctgagcg tagccgaagg caacattatt aaaccaacac catgaaagga tacatgtaca
     4141 ttctaaaatg ctcagatgat agttactatg taggtagtac taaaaacctt aaattgcgat
     4201 tacagcaaca tcagtcaggt gaaggagcca atcatactaa aaagcgactg ccggtggagt
     4261 tagtatatgt tgaagaattc caaagaatag acaaagcatt ttacagagaa aaacaagtgc
     4321 aaggctggag tagaaaaaag aaagaagcat taatagaaaa taaacctgaa aaactacatc
     4381 ttttagcaga atgtaaaaat gaaacgcatt tttccaatcg gtggttgagc ggagccgaaa
     4441 ccaccgctat aaaagagagc cttggcaata ccgacctttt aaagctacct aaaacagctt
     4501 ttttatgcag ccgacaagtt cctgcttcgg tggtattaaa atgcaatgac tgggcaatag
     4561 agcaaagaga gaaaaaggtg tgtgttatca gtggttttca cagccaaata gaaaaagatg
     4621 ttttgcatta tctgctcaaa ggcaaacagc ccatcatttt agcattagca agaggtctaa
     4681 aagaaaaatt ggaacctgaa tttgaaaagc aattggaaca aggcagactg ttaatcatca
     4741 caccgtttga caaatcggta aaacgagtaa gcagtcaaaa cgcacagaca cgcaaccaac
     4801 taatgacaag ccttgcagac caaatcacca tcggttacgc aagcccgggc ggtcagttgg
     4861 aagcgctact aaaaggtatt gagaaaccaa taaacaaaat tgtatgagcc aacgtcccga
     4921 tagttttcgg gacaacacta agtattgtag ttgggacagg aataagaaag cataaagtaa
     4981 accaccccta ccctatccca ctgaactgct ttaaaaaacg aacatcattt tcagaaaaaa
     5041 ttcgcaggtc gttgatgtta taaaccaaca tagtaattct ttctataccc ataccgaagg
     5101 caaaaccgct gtaaacattg ctgtctattt tacaattgtc gagaacggca ggatcaatca
     5161 tgccacagcc aagaatttct acccagcctg tgtatttaca gatgttgcag ccttcgccct
     5221 tacagaaatt acaggatata tccatctctg ctgatggctc tgtaaacgga aaatacgaag
     5281 ggcgtaaacg aatttcagtg tcagcaccaa acatctctct tgcaaaatag agtaatgttt
     5341 gtttcaggtc ggcaaacgaa acatctttgt caatgtagag tccttctacc tgatgaaaaa
     5401 aacaatgtgc ccttgctgaa atcgcttcat tacggtaaac tcttcccggt gatattgtac
     5461 gtaatggggg tttgtgtttt tccattattc tcacctgtac agatgatgta tgtgtacgca
     5521 gtgcaaggtc agggtttttg cggataaaaa aagtatcctg catgtcgcgg gccggatgct
     5581 cttcaggaaa attgagtgct gtaaaattat gccagtcatc ttctatttca ggtccatcag
     5641 aaactgtgaa tccaacagca ctgaaaattt cgataattct gtttctgata attgatagcg
     5701 gatgcaatgc accttcactc attggctcac cgggcaaggt aatgtctatc ttcttttcgt
     5761 cagtagtaac gttctcaaat ttctctttaa aactttcgaa gatggcttct gtcttttgtt
     5821 tgagcaagtt aacctgcaac ccgtaatttt ttctttcggc aggttcaata tttttcatca
     5881 atgcaaacat atctgccaac acacctttct tagaaagata tttaagacgg aaattttcaa
     5941 gatcggcagg tgactgaatt gaaaatgtct ctgcctcctg aatatgctgc tcaattaatt
     6001 cttgcgacat aagataaaat aagataatgc aaattaacga ttaaaacttt tgcttatgat
     6061 gttaatattc tattagaaat aatgacaaat aaaactattg tattaatcaa cacctgcacc
     6121 atgacagttt ttaaatttct ttccactacc acaagggcaa gggtcattac gtcccacctt
     6181 tatttctgcc tttgcaggtt gttgctttgg tggtggcggt ggtccttgcg gacggctttg
     6241 ccctcctgca tctgattcgt tgtggcttgt ttgcattttg ctcatatcag tacgttgctg
     6301 ctgcacttta gcttcacgta cattttcctg attttgaaca gggatatagc ttttaatcaa
     6361 aaacgaaaca atttctttat tcaccctgtc aatcatctgc ttgaaaagtt caaacgactc
     6421 aaacttatat atcaacaaag gatctttctg ttcgtaaact gcattttgta cactctgacg
     6481 caagtcgtcc atttcgcgca aatgctctgt ccatgcttcg tcaataaaat tgagtaccga
     6541 tgatttttcg agagcagaaa tcaactcttt acattttgaa tcgtacgact tctttagtcc
     6601 tgcaattaca ttcattgcct tagtaccatc actaaatggc acaacaacat ctacaattgt
     6661 ctgaccctga ttagtgtaaa tattttcaat aacaggaaat gctttttctg caataagatt
     6721 tgatttctgt cggtaatggc cgtttgcttt ttcgaaaacc atttgtgtca ggtcttcgag
     6781 ttttatctgc aaaaatgctt ttgcatctat gggtgcttca attgacagat aacgaataag
     6841 gtctaactgt aatgcgtcaa aatcgcgctc ctgattatag ctgcttataa tgctttcgca
     6901 aacatcatat tgcatgttca taatgtcgag tgcaagcctt tctccaaaca atgcatgacg
     6961 tcttttctta tagataactt cgcgctgtgc attcatcaca tcatcgtatt cgagcaaacg
     7021 tttacgtatt ccgaagttat tctcttcaac cttcttctgt gcacgttcaa tagatttggt
     7081 aatcatgcta tgctgaataa cctcaccctc ttcaatgccc attctgtcca ttagtttggc
     7141 aattctttca ctgccgaaca aacgcattaa atcatcttct aacgaaacaa aaaactgtga
     7201 tgaacccggg tcaccctgac gacctgaacg tccgcgcaac tgtctgtcaa cacgtcttga
     7261 gtcatggcgt tctgtaccga taattgcaag accaccggct tcttttactc cctgacctaa
     7321 tttaatatct gtaccacgac cggccatgtt ggtagcaata gttaccgttc cagcctgccc
     7381 tgcttctgca acaatatccg cttctttctt atgtaactta gcatttaaaa cattgtgctt
     7441 aatatttctt tgcttgagca tacggctaag caattcagaa atttcaaccg atgttgttcc
     7501 tacaagaacc ggtcttcctg cagctgttag ttttgcaatc tcttcaataa cggcattgta
     7561 tttttcacgc ttggttttat aaaccaaatc ttctctgtcg tcacgaataa tttttttgtt
     7621 ggtaggaata actactacat ccaacttata tatctgccaa aattctccgg cttctgtttc
     7681 tgcagtacct gtcataccag ccagtttgtg gtacatacgg aaatagttct gcaaggtcac
     7741 cgttgcatac gtttgtgtag cagcttcaat ctttacacct tctttagctt caattgcctg
     7801 atgtaaacca tcggaatatc ttcttccttc aagaacacgt cctgtttgct catcaacaat
     7861 tttcactttt ccatctgcga taatatactc atcatctttt tcgaaaagcg tatatgcttt
     7921 tagtaactga ttgatggtgt gaatacgctc tgattttaca gagaagtcgc gcatcagggc
     7981 ttctttgcgt tctaactttt cttcgttagt tccattagat ttttcaattt ctgcaataga
     8041 agcaccaaca tcaggcatca caaaaaagtg tgtgtcttcg ttgttggcag taatcagttc
     8101 aatacctttc tctgtaagtt caacggtatt atttttttcg tcaatcacaa aaaacaattc
     8161 tgcatctact ttatgcattt cctttgactg atcctgcatg tagtggtttt ctgttttctg
     8221 caataatgct cttacaccct gctcacttaa aaactttacc aatgctctgt ttttgggcag
     8281 acctctgtaa gctcttatca agggcatgcc tgcttcatcg ggtttatttt ctcccaattt
     8341 ctttttagca tcggcaatta cagtgttgat ataatttctt tgtgcattaa acagtatttc
     8401 aactttgggt ttcaatgcaa aatattcctg agtgtcgcct ttaggtgttg gacctgaaat
     8461 aatcaagggt gttcttgcat catcaaccaa tacactatcc acctcatcta caattgcaaa
     8521 atgatgtggt cgttgtacca tttcatgagc atgacgcgtc atattatcac gcagataatc
     8581 gaaaccaaat tcattattag ttccgtaggt aatatcagct aaatatgcct tacgtctttc
     8641 ttcagtgttg ggttgatgtt tatcaataca atcaacagaa agtcctaaaa attcaaataa
     8701 cggaccattc cactcagcat cacgctttgc aagatagtcg ttaacagtaa caacgtgcac
     8761 acctgcacct gccaaggcgt tgagataaat gggtaatgtt gcaacaagcg tttttccctc
     8821 accggttgcc atctctgcaa tttttccctg atgcaaaaca gagccaccta taagctgcac
     8881 atcataatga atcatgttcc agagaacctt acttcctgct gctgtccagg tattgctgta
     8941 tattgccttg ccatcttcta ttctgatacc atcgcgttta gttaccagat ttttatcatt
     9001 gtcattggct tctactgcca gctcggcatt ctcagtaaaa cgtttggcaa cttcgcggac
     9061 aagtgcaaat gcttcaggct gaatttcgtt cagcacctct tctgtcttct tatttctctc
     9121 tttctttaaa gcatcaatgc ggttgtaaat ttcttctttt tggttggcat ccatttcagg
     9181 atcggcttca gcttcttcgt taagctgagt aagttctgta tcaatctctg aaagatagtc
     9241 ctgtatgcgc gtacgaaatt catcggtttt attgcgtaat tcatcattgc tgagtgatga
     9301 catgttttcg gctactgcat ttactgcttc aattacaggt gacagtgctt tgtaatcgct
     9361 ttcggcctta ctgccaaaaa ttttggaaat agttttattg ataaaatcaa tcattgttgc
     9421 tgattataat gtgctaaaaa taaaaggtga caaaagtaac gtaaag
//
LOCUS       DAORQT010000043         9443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_969_length_9443_cov_14.289199, whole genome shotgun sequence.
ACCESSION   DAORQT010000043 DAORQT010000000
VERSION     DAORQT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_9443_cov_14.289199"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..840
                     /locus_tag="PK134_02810"
     CDS             358..840
                     /locus_tag="PK134_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRB37810.1"
                     /translation="MAEAEFLKTEKEIFTEYMISNGLRKTPERYAVLEEAYRTNGHFN
                     VDMLFELIQKRKLRVSRATVYNTIEHLLKCGLITKHQFGETKFIYEKSFAYKQHDHLI
                     CGDCEKVFEFCDPRLYQIQSTVEKYLDFKITQHALNFYGQCNKLKQTGACEHFKVKKQ
                     "
     gene            837..1178
                     /locus_tag="PK134_02815"
     CDS             837..1178
                     /locus_tag="PK134_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRB37811.1"
                     /translation="MNFSFTTSEKGNFCIIKLSGNLMEKGQANELMDEINTCIIRDMK
                     TFVLDLADLRFMSSSGLTVLLNILTKSRKAGGDTIICNINSQLKSVFTITRLTEVFTI
                     TENQEAAIAAI"
     gene            1193..2461
                     /locus_tag="PK134_02820"
     CDS             1193..2461
                     /locus_tag="PK134_02820"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRB37812.1"
                     /translation="MKIDVLLGLQWGDEGKGKLIDVLAPDYDVIARFQGGPNAGHTLE
                     FDGIKHVLHTIPSGIFRENKLNIIGNGVVIDPLIFKKENDSLLKLGIDIKKNLWVSRR
                     AHLILPTHRLLDAASETMKGKTKIGSTLKGIGPTYMDKTGRNGLRVGDIERHDFKDAL
                     NYLLNKHAELLSFYNHPTVSKEDLEMWLESISTLREMTLVDSEYEINSLINSGQRILA
                     EGAQGSLLDIDFGSYPFVTSSNTITAGACIGLGVPPSAIGKVFGVFKAYCTRVGSGPF
                     PSELNDATGDELRKKGNEFGSTTGRPRRCGWLDLPALKYTAMVNGVTSLMMMKSDVLS
                     GFENLEVCNSYKVNGQSSVRMPFNVVNDIPEPEYITFKGWQNDISKANSFESLPSELH
                     NYIQAIEKYIGIPLDVVSVGPDRTQTIIRK"
     gene            2463..3185
                     /locus_tag="PK134_02825"
     CDS             2463..3185
                     /locus_tag="PK134_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37813.1"
                     /translation="MKIFSVFFFSLLICSNIYGQQLSIENKPALNEESKSVDSWVVHV
                     DQDMNFCFSAYSEFMRKNFDYRINRRSKIVHSVDKKLFTEISNLRIDQRAIFTQESKG
                     TAIAFQFSPGYDIHFGNTMYKEEFDRARAMVVNYVHFHYKMFYDDQIKNLRSKIKGLE
                     KNISNNQSKIEKNTKTVNLNAEDISNNAVGADKLREKNEKLEKDNVTLTTEIDKFRSQ
                     ITQLETEIDNANQSLKVVEAFN"
     gene            3194..4138
                     /gene="trxB"
                     /locus_tag="PK134_02830"
     CDS             3194..4138
                     /gene="trxB"
                     /locus_tag="PK134_02830"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRB37814.1"
                     /translation="MSENSEHVKCLIIGSGPAGYTAAIYAARADLKPVMYQGLQPGGQ
                     LTTTTEVENFPGYPKGTMGPEMMEEFRQQAERFGTDIRWGYASSVDFKGPVHKVIIDE
                     EKEITADTVIIATGASAKWLGLESEQRLNGFGVSACAVCDGYFFRNQHVAIVGAGDTA
                     AEEATYLAKLCTKVYMIVRRDEMRASKAMQHRVMNTPNIEVLFNTETKEVVGEKTVSA
                     VKVVNNITGAERILDVTGFFVAIGHQPNTDIFKGWLDMDKTGYIITKPGSTKTNIEGV
                     FACGDAQDKIYRQAVTAAGTGCMSALDAERYLADKGIH"
     gene            4226..4867
                     /locus_tag="PK134_02835"
     CDS             4226..4867
                     /locus_tag="PK134_02835"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRB37815.1"
                     /translation="MTDTFKHQGLRRQLITELKSKGINDAAVIAAMTKIPRHAFMDNA
                     FIESAYIDKAFPIGSGQTISQPYTVAFQTLLLGIQKGDKVLEIGTGSGYQTAILCELG
                     AKVFSIERHKLLADKAKSLLEKLGYIPKIHFGDGFKGLPAFAPFDKIIVTCGAPDIPQ
                     SLFEQLKTGGVMVVPVGEDVQVMKTVVKKPDQTMEVSDFNSFRFVPMLGNKVR"
     gene            5051..5596
                     /locus_tag="PK134_02840"
     CDS             5051..5596
                     /locus_tag="PK134_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB37816.1"
                     /translation="MTDRELIEGCIRGDRNCQKALFEKFAGKMKVVCMRYVNNHEDAE
                     DVLQEGFIKVFKYISKFGFEGSFEGWIRKIMVNTALTKITRYSYKEEVALVHSNMPVY
                     DYGDVIAEMSAKELKAFINALPDGYRLVFNMYVIEGFSHKEIAESLSISEATSRSQLL
                     KARLALQEKLKKNKIAYSHAG"
     gene            5586..6905
                     /locus_tag="PK134_02845"
     CDS             5586..6905
                     /locus_tag="PK134_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37817.1"
                     /translation="MQDEQFDDLIRQKLNSEEESVSAGLWSRVEADLPVAKQGVFNRS
                     LLWLLLLFLIPAGAGWYYFTSNQTKISSLEGNRSSKQKVISNIASEVRNTDDKTMQNN
                     SVQSSDHNTLNNSIESNSSQIKYSSNANSTISANNNTVSKTLEPTYAISSTTNFNNQV
                     ENSEWDLLNPIQASLPEAASSQALISDKPTLEIKKTKRGIRVFADIYYAPEFAQHTRD
                     LGSSSPAYLKLRRNTETNIYSHSQGIRAGVWLSTNFFFKTGIDMFDMNERLSYTFIPG
                     MGNLPVGMRIDSINGYYINPLTNALVADTIVGSVNSTGEVIISNHYRFVNVPLMFGWR
                     FNKGLWSIQSSTGIYMNIYSGYKGKIIHESMQSLIDISSTSTPYKQNIGIGFSAEVIA
                     TYSIFKNIDVLIGPKYNYHLSDISRKGAPIQEKLSSIGMFSGIRYNF"
     gene            complement(6976..7560)
                     /locus_tag="PK134_02850"
     CDS             complement(6976..7560)
                     /locus_tag="PK134_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRB37818.1"
                     /translation="MKKLFLSAIAVTMAFSLNAQTQWSADKSHSKIGFTVTHMLIAEV
                     EGNFKDFTASMTSTKDNFEDSKVEFKAPVSSINTNDDKRDEHLRGSDFFDMANHPEIS
                     FTSVTFKPAGDKKFKVVGNLTMRGVTRSVTLEATHIGTVEDPWGNTKAGFKLTGKVNR
                     KDYGIAWNKTLTSGGAVVSDEVAMNCMIELIKQK"
     gene            complement(7888..8460)
                     /locus_tag="PK134_02855"
     CDS             complement(7888..8460)
                     /locus_tag="PK134_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37819.1"
                     /translation="MFIDFGAGQVRLLGNRRYEIIYNMQLHVVDDIVSETSKMEARAG
                     AGVATPEYAVDQALARLDLIDHILYHLTGFTTTGMRGMQCTGVELSSVESSPTHDILT
                     WAVRLQTDTAKKVMIEVEPEVVVIPNSHFPPNPTYSQSQTQLRLQSIPFGEFALLLNC
                     LAQVYFSVWLIFRVYVFLQTFLVVSLIPDR"
     gene            complement(8759..8941)
                     /locus_tag="PK134_02860"
     CDS             complement(8759..8941)
                     /locus_tag="PK134_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37820.1"
                     /translation="MQQKNKNPWRNILRGYYEMRKTLADDASRMALNEFLENFQRQGS
                     VNANGLFIPWKATGGG"
     gene            complement(9050..9313)
                     /locus_tag="PK134_02865"
     CDS             complement(9050..9313)
                     /locus_tag="PK134_02865"
                     /inference="COORDINATES: protein motif:HMM:NF037113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HRB37821.1"
                     /translation="MRDKIFINVGDVIKNPDEVWMTYYKNKSAQYRYVKFYGDKKTMI
                     VPVDVNMGMDENNKDNLLKIKTWFLLDKEEEKNKRVGVLIYKK"
BASE COUNT         3108 a         1567 c         1855 g         2913 t
ORIGIN      
        1 gataataaac ctgcaaaaag tgtggcaata aatagcctga aaaacgattt caatgtcaaa
       61 acagtgttgt atggttcaat tggcaaaagt acttgatata attctaaaac attgcatttt
      121 tgttgccgtt ggcattaaat atttttttac gacaaaaaat gaattttgtt attgttgtga
      181 tataggtcta ttgaggtttt tttcatttaa aaatcagaga taagcggaaa gcaatttatc
      241 aacaatttgg aagaaaaagt aagtgaagaa caactatttg tatctcagtt gtaattggaa
      301 attctataac agtttgattt attacttttg tagcaattaa aatggcattt atttaacgtg
      361 gcagaagcgg aatttttaaa gacagagaaa gaaattttta cagagtatat gataagcaat
      421 gggctacgca aaacacctga acgttatgca gtacttgaag aagcttatcg cactaatggt
      481 cattttaatg tggatatgct ctttgaactg attcaaaaac gcaagttaag agtaagccgt
      541 gccaccgttt ataatactat tgaacattta ttgaaatgtg gcttaataac caaacatcag
      601 tttggcgaga ccaaatttat ttacgaaaag tcttttgcct ataagcaaca cgatcatttg
      661 atttgtggcg attgcgaaaa agtatttgaa ttttgcgacc cgaggcttta tcagattcag
      721 tcaacagtag aaaaatattt agatttcaaa ataacacaac atgccctgaa tttttatgga
      781 cagtgtaata aactcaaaca aacaggggca tgcgaacatt ttaaagtaaa aaaacaatga
      841 attttagttt taccacatca gaaaagggaa atttctgtat tattaaactt tcgggcaacc
      901 tcatggagaa aggacaggct aatgaactga tggacgaaat caatacctgt attatcagag
      961 atatgaaaac gtttgtgctc gatttagcag atttgcgttt tatgagcagc agcggactta
     1021 ctgttttatt aaacatcctg accaaatcgc gcaaggccgg tggcgacacc attatttgca
     1081 atataaatag tcagttaaaa tctgtattta ctattacacg attgacagag gtttttacca
     1141 taacagagaa tcaggaagct gctattgctg ccatctgaat aaaaaaataa ttatgaaaat
     1201 agacgtatta cttggacttc agtggggcga tgagggcaaa ggaaaactga tagatgtttt
     1261 agctcccgac tatgatgtga ttgcacgttt tcagggtggg ccaaatgcag gacatacctt
     1321 ggagtttgat ggaataaaac atgtgttgca taccattccg tcaggaattt ttcgtgaaaa
     1381 taaacttaac atcattggta atggtgtggt aatagatccg ctgattttca aaaaagaaaa
     1441 tgattcactg cttaagttag gaatagacat aaagaaaaat ctttgggtat cgcgaagagc
     1501 acatctcatt ctgccaacac atcgtttgct tgatgctgca tcagaaacca tgaagggtaa
     1561 aacaaagatt ggttctacac taaaaggaat aggtccaacc tatatggata aaaccggacg
     1621 taacggtctg cgcgttggcg acattgaaag gcatgatttt aaagatgcat taaattattt
     1681 attaaacaaa catgcagaac tgctttcatt ctacaaccat ccaacagtca gcaaggaaga
     1741 cctcgaaatg tggcttgaga gtatttcaac acttcgcgaa atgacattgg ttgacagtga
     1801 atatgaaata aacagtttga ttaattccgg ccagcgtatt ttggcagaag gggcacaagg
     1861 ctcattgctt gatattgatt ttggatctta cccatttgtt acgtcaagca atacaatcac
     1921 agcaggtgca tgtatcgggc ttggtgttcc tccttcggca ataggtaaag tgtttggtgt
     1981 gttcaaagca tattgcacac gtgtaggcag tggtcctttc ccatctgagt taaatgatgc
     2041 aacaggagat gagttgcgta aaaaaggcaa tgaatttggt agtactaccg gtcgcccaag
     2101 acgatgcgga tggctcgatt tacctgcttt gaaatatact gcaatggtta atggcgttac
     2161 aagtctgatg atgatgaaat ctgatgtact tagtggtttt gaaaacttag aggtttgtaa
     2221 ttcttataaa gttaatgggc aatcttctgt acgaatgccg tttaacgtag tgaatgatat
     2281 tccggagccg gaatacatta cctttaaagg atggcagaat gatatcagta aggcaaatag
     2341 ttttgaaagt ttaccatctg aattgcacaa ctacattcag gcaattgaaa aatacatagg
     2401 tattccatta gatgttgtat cagttggccc cgatagaacg caaacaataa tcagaaaata
     2461 aaatgaaaat attttcggta ttttttttca gcttacttat ttgctccaac atttacggac
     2521 agcaactgtc aatagaaaat aagcctgctt taaacgaaga gtcaaagagt gttgattcgt
     2581 gggtagtaca tgtagatcag gatatgaatt tttgtttctc tgcttacagt gagtttatgc
     2641 gtaagaattt tgattatcgt attaaccgca gaagtaaaat tgttcattca gtagataaaa
     2701 aattatttac cgaaatttca aatctgcgga tagatcaacg agcaatattt acgcaagagt
     2761 caaaaggtac tgcaatagct tttcaattca gccccggcta tgatatacat tttggcaaca
     2821 ccatgtataa ggaggagttt gaccgtgcaa gagccatggt tgtaaactac gtgcattttc
     2881 attacaaaat gttttatgat gatcagataa aaaacctgag atcgaaaatc aaaggattag
     2941 aaaaaaatat cagcaacaat cagtcaaaaa ttgaaaaaaa tactaaaact gttaatctta
     3001 atgcagaaga tatttcaaat aatgctgtag gagcagataa gttgcgcgag aagaatgaaa
     3061 agctagaaaa agataatgtg acactaacaa cagaaataga taaattcaga tcacagataa
     3121 cacagttaga aacagaaatt gacaatgcta accagagcct taaagtagtt gaggcattta
     3181 attaaaatat gcaatgagtg aaaatagtga acatgtaaag tgcctgatta ttggctcggg
     3241 tcctgcagga tatacagcag caatttatgc agctagggca gatttaaaac ctgtaatgta
     3301 tcagggattg caacccggag gtcagttgac aacaacaaca gaagtagaaa atttcccggg
     3361 ctaccctaaa ggtacaatgg ggccggaaat gatggaagaa tttcgtcagc aggcagagcg
     3421 atttggcact gacatcagat ggggatatgc atcatctgtt gattttaaag gacctgtaca
     3481 taaagtaatc attgacgaag aaaaggaaat tacagccgat acagtaatta ttgcaacagg
     3541 tgcatcagct aagtggcttg gattagagag cgaacaacga ctgaatggtt ttggtgtttc
     3601 tgcatgtgcc gtttgtgatg gatatttttt ccgtaatcag catgtagcaa ttgtaggggc
     3661 aggtgatact gctgccgaag aagcaacata tctggcaaag ttgtgtacaa aagtttatat
     3721 gattgtgcgc agagatgaaa tgcgtgcatc aaaagcaatg caacacagag taatgaacac
     3781 accaaatatt gaagtcttgt ttaatacaga aactaaggag gtggtaggag agaaaacagt
     3841 gtctgctgtt aaagttgtta ataatataac aggtgcagaa cgcatacttg atgtaacagg
     3901 cttttttgtg gcaattgggc atcaaccaaa taccgatatt tttaaagggt ggcttgatat
     3961 ggataaaacc ggttatatca taaccaagcc gggctcaacc aaaacaaaca tagaaggtgt
     4021 ttttgcttgt ggcgatgcac aggataaaat ttacagacag gctgttactg ctgcaggaac
     4081 aggttgtatg tctgctttag atgcagagcg gtatttggca gataagggca ttcattaaaa
     4141 aaatcattag catttttttg ttaaaaattt tattgattca gcaataaaca ttgttgagtg
     4201 aatggtaaat ttgtttgcaa tgaatattac agacactttt aagcatcagg gattaagacg
     4261 acagctaatt acagaattaa agtctaaggg gataaatgat gcagccgtaa tcgctgccat
     4321 gacaaaaatt ccacgtcatg catttatgga taatgcattt atcgaatctg cctatattga
     4381 caaagctttt cccattggtt caggtcaaac aatttcacaa ccctatacgg ttgcttttca
     4441 aaccttgttg cttggtattc aaaaaggtga taaagtgttg gaaattggaa caggctccgg
     4501 ctatcagacg gctatacttt gtgagttagg tgcaaaagta ttttctattg agcggcataa
     4561 attacttgcc gataaagcaa aaagtttact cgaaaaattg ggttatatcc ctaaaataca
     4621 ttttggcgat ggctttaaag gactgcctgc atttgcacca tttgacaaga taattgttac
     4681 ctgtggcgca cccgatattc cacagtcatt gtttgagcaa ttgaaaaccg gaggagtgat
     4741 ggttgttcct gtaggtgaag atgtgcaggt aatgaaaacg gttgttaaaa aacctgatca
     4801 aaccatggag gtttcagatt tcaactcatt ccgctttgtt cccatgcttg gtaataaagt
     4861 aagataagta cattatagtt ttattaaaaa acattcacat tttttgagta atatttttct
     4921 ctcacttaga tttatggcaa gtgaaggtgg tgttttgcta tgttttaatt aattttacca
     4981 aagcgcaaaa gtcaggcaac gtttcccgga cttattgagt ctttatatat gaaatgcatt
     5041 caatactgtt atgacagacc gtgaactaat tgaaggttgt attcgcggtg acagaaattg
     5101 ccagaaagct ttattcgaaa agtttgccgg taaaatgaaa gtcgtttgca tgcgctatgt
     5161 caataatcac gaagatgcag aagatgtttt acaagagggg tttataaaag tgtttaagta
     5221 tatatcgaaa tttggttttg aaggatcttt tgaaggttgg ataagaaaaa taatggttaa
     5281 tacagcgctt acaaaaatta cacgctacag ttataaagaa gaagttgcct tggttcatag
     5341 caacatgcct gtttatgatt atggtgatgt gattgcagaa atgtctgcca aagagctgaa
     5401 agcattcata aatgcattgc ctgatggtta taggctggtg tttaacatgt atgtgataga
     5461 aggtttttcg cataaagaaa tagctgagag cctgagtatt tcagaagcaa catcgcgtag
     5521 tcagttattg aaagcacgat tggcattaca agaaaaatta aaaaaaaata aaatagcata
     5581 cagtcatgca ggatgaacaa tttgatgatc tgataaggca gaaacttaac tccgaagagg
     5641 agtcagtaag tgccggactg tggagtaggg tagaagcaga tttacctgtt gccaaacaag
     5701 gagtttttaa tagaagttta ctatggttgc tacttttatt tttaattcca gcaggtgcag
     5761 gatggtatta ttttacttca aatcaaacaa aaatttcttc attagaaggt aatcgttcaa
     5821 gcaaacaaaa ggtgattagt aacatagcat ctgaggtaag gaatacagac gacaaaacga
     5881 tgcaaaacaa ttctgttcag tcatcagatc ataatacttt aaacaattca atagaaagta
     5941 attcgagtca aattaaatat agctctaacg caaatagcac tatctcagct aataataata
     6001 ctgtctcaaa aactttagaa ccaacctatg caatttcatc aacaacaaat ttcaataatc
     6061 aggttgaaaa tagtgagtgg gatttgctta atccaataca ggcatcattg ccggaggcag
     6121 caagtagtca ggctcttatt tcagacaaac ctactttaga aataaaaaag acaaagagag
     6181 gaattcgtgt atttgcagat atttattatg cacctgagtt tgcccaacat acacgcgact
     6241 taggaagcag cagccctgcc taccttaagt tgaggcgcaa tacagaaaca aacatttatt
     6301 ctcacagtca gggaattagg gcaggtgttt ggttgagtac caatttcttt tttaagacag
     6361 gaattgacat gtttgatatg aacgagcgac tgagctatac atttattccg ggcatgggaa
     6421 atctccctgt aggaatgaga attgattcta tcaatggtta ttacatcaat ccactcacaa
     6481 acgctttagt tgctgatacg attgtaggtt cagtaaattc tacaggagaa gtcatcattt
     6541 caaatcatta tcgttttgtc aatgtacctt taatgtttgg atggcgattt aacaaaggtt
     6601 tatggagcat tcagagcagc acgggaattt atatgaatat ttattcaggg tataaaggaa
     6661 aaatcataca tgaaagcatg caatcgctaa ttgatatttc ttccacctca acaccttata
     6721 aacaaaatat tggtataggt ttttctgcag aagtaattgc aacatacagt atctttaaaa
     6781 acattgacgt gctcattggt cctaaatata attatcattt aagcgacatc agtcgcaaag
     6841 gtgcacccat tcaagaaaag ttgagtagca taggtatgtt ttcaggtatc agatataatt
     6901 tttgatttag gtttggatta attgaataaa aaaggcagcc ttttcagagg ctgcctgcaa
     6961 aaaattaatt cacatttatt tttgtttgat cagttctatc atacaattca tagctacttc
     7021 gtcactcacc acagcaccac ctgatgttaa agtcttgttc caggctatac cataatcttt
     7081 acggttaact tttccggtta atttaaagcc ggcttttgtg tttccccatg gatcttcaac
     7141 agtacctata tgcgttgctt ctaaggttac acttcttgtt acaccacgca tggtaaggtt
     7201 accaacaact ttgaattttt tatcacctgc aggtttaaaa gttacactgg taaaagaaat
     7261 ttctggatga tttgccatat caaaaaagtc tgacccacgt agatgctcat cacgtttatc
     7321 gtcattggta ttgatagacg aaacaggtgc tttaaactca actttgctat cttcaaaatt
     7381 atcttttgta gaggtcattg aagccgtgaa gtctttgaaa tttccttcta cttcagcaat
     7441 aagcatgtgt gtaacggtaa aaccgatttt agaatgactt ttgtctgccg accattgtgt
     7501 ttgtgcatta aggctgaaag ccatagttac tgcaattgca cttagaaata attttttcat
     7561 tttttatatt ttttgattta tggtgcaatg atagcccaac gataaacatt aaatattaac
     7621 ctagattaac ttcaaaaaaa ttaattaaaa tttcagtttt atatttgtaa caacatataa
     7681 gatattatgc agttgcaaca atgtcataaa aatattacta ttgaattatt aatccgaaag
     7741 atgtgataaa gttttttcta aaagcatggt tagtgttagt tttgatttgt attgtcattc
     7801 tcttttatga tgactataaa aaagaaaaac agagagagta tgaaaggtca ttacctaaac
     7861 aacctgaatg ggatacagca acatcaccta cctatcagga attaatgaga caactaagaa
     7921 agtttgaaga aagacataaa cacgaaaaat taaccaaaca gaaaaatata cctgtgcaag
     7981 acagtttaac aaaagagcaa attcgccaaa gggaatggat tgaagacgaa gttgagtctg
     8041 agattgagaa taggttggat taggaggaaa gtgactattc ggaataacca caacttccgg
     8101 ctctacttca atcattacct tcttggcagt gtcagtctgc aagcgtacag cccatgttag
     8161 tatatcatgt gtggggctgc tttccaccga acttaattca acacctgtgc attgcatgcc
     8221 tctcatgcct gttgtcgtaa atcctgtaag gtgatataat atgtggtcaa tcaggtcgag
     8281 gcgagccaat gcctggtcaa ctgcatattc aggtgtggca acaccggcac cggcacgcgc
     8341 ttccatcttg cttgtctcac tcacaatatc atccaccaca tgcaattgca tattatatat
     8401 aatctcatat cgtctgttgc cgagcaatcg aacttgacct gcaccaaaat caatgaacac
     8461 tgccttacgt gggaatggta atgagttggc attttatttg tatatttgtt attcaattaa
     8521 gcggttaatc ttgcaagggg tgaggggctt taaatgtagt tccatcccgc ttcacaatag
     8581 tgagattaac cgtttatttt tttataaata agtattccct tccttacatt gccctcatct
     8641 ttatctaact tgtaccacga ctttatatta agtcttccgt ccacttcaac cggcaccggc
     8701 atcattgtag aatttaacgt atgtgtattt tgccgccaaa aatggctgat gctttgcctt
     8761 agccaccacc ggttgccttc caaggtataa ataagccatt agcattaaca gacccttgtc
     8821 tttggaaatt ttccaaaaat tcatttagtg ccattcgact ggcatcatcg gccagtgttt
     8881 tgcgcatttc ataatatcct cgtaatatat tgcgccacgg atttttattt ttttgttgca
     8941 ttggtatgat ttttatttta tatttgtatc cgaaacagca aaatctgttg ctttggtctg
     9001 atgttggcgt aggtaacgcg agggatgatc atcaacagat ttagctgttt cactttttat
     9061 atattaatac tcctaccctc ttattttttt cctcctcttt atctaaaaga aaccatgttt
     9121 ttattttcaa taagttatcc ttgttgttct cgtccattcc catattaaca tctaccggaa
     9181 caatcattgt ttttttatcg ccataaaatt taacatatcg gtactgagcg gatttatttt
     9241 tatagtaggt catccaaacc tcgtctgggt ttttgataac atcacctacg tttataaata
     9301 ttttatcgcg catttcctta tcacttaatt atttattgcc tttcgcatgg ggcgttatca
     9361 tttgctttat tcgcaagtgt ttttattgtt taaggcgctc atgaacctcg ttcctcggtt
     9421 tcaactttta ggatagacaa ctt
//
LOCUS       DAORQT010000044         9025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1057_length_9025_cov_7.231773, whole genome shotgun sequence.
ACCESSION   DAORQT010000044 DAORQT010000000
VERSION     DAORQT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1057_length_9025_cov_7.231773"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PK134_02870"
     CDS             <1..1027
                     /locus_tag="PK134_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRB37822.1"
                     /translation="EYLPACLPVAGISNIPGGKEYYNWLANKWTTTTMSPDEIYNLGL
                     KEVERLHSEMDKVKAETGFKGSLKEFFHFIHTDPQFFPFKKDSDVIAAYLAIEDRMKP
                     QLKKLFNHVPKAAFEVRQTEAYRAASASAEYNTAAPDGSRPGVFYVPILDATKYNVVG
                     MEDLFLHEAIPGHHYQISLQQENTSLPEFRRFGWFGAYGEGWALYTESLGKELGLYTD
                     PYQYFGCLSEEMHRAIRLVVDVGIHLKGWTREKAIQYSLDNEMESEADITAEIERYMA
                     IPGQALSYKIGQLKIIELRQKAEEQLKDKFSIQKFHDQLLSYGCLPLSVLEKSIKLWI
                     KEQKKQG"
     gene            1110..2096
                     /locus_tag="PK134_02875"
     CDS             1110..2096
                     /locus_tag="PK134_02875"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRB37823.1"
                     /translation="MKQLIENFPSQMREAMTIGETFKATSWPHEIKNVLVTGLGGSGI
                     GGTIVSQLCEKEMPIPFMVNKDYFMPAFVNKHSLVVVSSYSGNTEETLHAMDMAIKQG
                     AKVVCVSSGGKTVELAKQHGLDHILIPGGMPPRSCYGYSSTQLFYILHAFKLIGDGFK
                     ASLKNAVDLLYAESANIKNASHKLASELIGKIPVIYSASNNEGVVVRFRQQVQENGKM
                     LCWHHVFPEMTHNELVGWTQPHNDIAVVLFRDKDDFFRTAKRMDICKEIFGRYTPHIY
                     EVWSKGGSPIEKAIYLIHFGDWTSWYLSEIRNIDCTEVKVIDYLKSEMAKLN"
     gene            2121..2750
                     /gene="tmk"
                     /locus_tag="PK134_02880"
     CDS             2121..2750
                     /gene="tmk"
                     /locus_tag="PK134_02880"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRB37824.1"
                     /translation="MKKENLFIAVEGIDGSGKSTQVQLLKANLEEAGYKVYATNEPTN
                     FEAGKLIRSIFNHRVNADQHTIAALFVADRLQHLLDKENGILAMLEKGYTVITDRYYF
                     SSYAYHSAYVEMDWVMSINAKCAALLKPDLNIFVDVDPETAFKRIIATRTSTELYETK
                     ENLRKVYDNYLKAFKLLKNTESIVFVNGKNDVQQVAADIFKTVKNLLHT"
     gene            2813..4447
                     /locus_tag="PK134_02885"
     CDS             2813..4447
                     /locus_tag="PK134_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRB37825.1"
                     /translation="MNKKHPKALPYLFLSEMWERFGFYLMLGIFFLYMTDTQRGGMAM
                     DRKEASDIFGTFIALVYLTPFVGGLIADRVLGYRVSITLGGILMGIGYCGLAIPGMTA
                     FYVSLLLIIIGNGFFKPNISTILGNVYNDPEYKPLKDSGYNIFYMGINIGAFICNFFG
                     SYLRNNFSWGAAFAAAGIGMFVGVIIFWIGNKHYAHADVRKPVKPEDMPVSKILGITL
                     LPALIAGYIGWIIPENIFGSDSTDAFILGALPVAGYYVNILLRSDKEDRRPLAALLAV
                     FAVAIVFWAVFKQNGTALTTWAESYTNREVPQSVEPVAKVLGVTQTVTFSKDSVPLTD
                     SQFRVARDENKKAIKTWDYPLYYKNVPADKLPKEGESLTLISTELGQSINPFWVVILT
                     PVIVAFWAFLKRRNSEPSTTWKIFYGFLITSLSTLVMVAATYACHNGLEKSSMWWLIA
                     SYGVITVGELCLSPMALSLVSKLSPPRLTALMMGGWFLSTSIGNKLSGVLASMWDEYE
                     HKANFFLVNFTLVMITTIIILLMLKWLNKIVKEHGA"
     gene            4659..7178
                     /locus_tag="PK134_02890"
     CDS             4659..7178
                     /locus_tag="PK134_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37826.1"
                     /translation="MRLLSTAVILLGCLCAQAQISITNNEMPSAGDTARFTKAVVNPL
                     INFSATGTNHTWSFGNLRNNGQDLKSYQNVSSTGFIYSLFFSNLPFNPNRANVTEPGQ
                     ALPSNPLLTITDPYNFYYRSSSVYKQVGFGAEIANIPMPVAFSQHDIIYNLPLNYNNS
                     DTSHSAWNIGLPGLGYYGLTQTRINHVDGWGTLTTPHGTFNTLRVKTQLYIKDTISVD
                     TLNLNFALNRPKTTQYKWLANNEIIPVLQITTTEILGLEVVTEIIYRDDYNTVSPGSL
                     NAAYCAGSNVSIPYTATGSFNGSGFLQTANSFTAQLSDATGSFANAVNIGSVTSRVSG
                     NINAVIPANTPVGNGYRIRIISSSPALTGGDNGFDLRIENNAVASIVNQGLSAFCSGN
                     SALLQSVNVDSGYTYQWQLNGTDISGATTDNYLASATGNYTLNITNSCGTITSNAAAI
                     NVLPMPTVQVTASGPLTLCLGTTVTLSTLFDSTYSYQWQLNGVDISGATTENYTAASG
                     GDYTVVVSNSCGNVTSSVQTVSVQSAPVAGSLTAASSTICHGDSVQINYSLQSAGITW
                     LHNGNILSGITTNSHFANSAGVYSVVESDLCGSDTSVAVILTADSLPTAVITASGALS
                     FCAGGDVVLTAATGNNYAWQWYMNGTPLATTQLITATQSGNYEVEITNACGSVMSTLV
                     AVTAFPLPVVPTITLSNDSLLATTTVGTYQWFYNGNAITGATNDFYVPSQNGNYSLMV
                     TDANGCSSMSAVYNMTTVGLQTIFKQAHNVFPNPFNDYLTIELPQTENAIILTVEGKQ
                     LWSGTLHQGKNVLNMQQFVSGMYILQLNNENGITNYKLVKR"
     gene            complement(7224..7886)
                     /locus_tag="PK134_02895"
     CDS             complement(7224..7886)
                     /locus_tag="PK134_02895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRB37827.1"
                     /translation="MKKYIALVSVILILDQCLKFWIKTNMYLGQEYHIAGNWFIIHFT
                     ENNGMAFGMELFGKKFLSLFRIGVMFALAWYVWHLHKTKAHHIYTTAMALIFAGAVGN
                     IIDSIFYGRLFSSSEFGVATFMPDAGGYAGWLHGKVVDMFYFPIIQTHYPQWFPLWGG
                     EEFIFFRPVFNIADSAITAGVLLIILFNRTIFKHIETTPETEQTTVEMRSEELNTANH
                     NS"
     gene            complement(7958..8350)
                     /locus_tag="PK134_02900"
     CDS             complement(7958..8350)
                     /locus_tag="PK134_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRB37828.1"
                     /translation="MENSPDKTRYSDAELNEFRELIIQKLTDAKVELNNLQAFLNNSN
                     EHGTDDTASSFKMLEDGSDTLAKEEAGQLAARQRKFIEQLENALVRIENKTYGLCRVT
                     GKLIPKERLRAVPHTTQSIEAKVQQYRD"
BASE COUNT         2800 a         1578 c         1756 g         2891 t
ORIGIN      
        1 agaatatttg cctgcttgtc ttcctgttgc cggtatttct aatattccgg gaggcaaaga
       61 atattataac tggcttgcga ataaatggac aacaacaaca atgagtcctg atgagattta
      121 taatttaggc cttaaagaag tcgaacgttt acattcagag atggataaag taaaagcaga
      181 gacaggtttt aaaggatcac ttaaagaatt ttttcatttc attcatactg acccacaatt
      241 ttttccattt aagaaagatt ctgatgtaat tgcagcctat cttgcaatag aagacagaat
      301 gaaaccacag ttgaaaaaac tttttaatca tgtgcctaag gcagcttttg aggttaggca
      361 gacagaagct tatcgtgcag catctgccag tgcagagtat aacacagcag cacctgatgg
      421 atcaagaccc ggggtttttt atgtacctat tttagatgca acaaaatata atgttgtagg
      481 tatggaagat ttgtttctgc acgaagccat tccaggtcat cattatcaga tttcattaca
      541 gcaggaaaat acatcactac ctgagttcag gcgctttggt tggtttggag cctatggcga
      601 agggtgggct ttatatacag aatcattagg gaaggaatta ggattatata cagatcccta
      661 tcagtatttt ggttgcctga gcgaggaaat gcacagagcc attcgtttgg ttgtagatgt
      721 gggtattcac ctgaaaggat ggacgcgcga aaaggcaatt caatattcat tggataatga
      781 aatggaaagc gaggctgata ttactgcaga aattgaacgc tacatggcca taccgggtca
      841 ggcattgtcg tataaaatag gtcagttaaa aattattgaa ttacgtcaaa aagctgaaga
      901 gcagttaaaa gataaattca gtattcagaa atttcatgat cagctacttt cctatggttg
      961 tcttccatta agtgtactcg aaaaatccat taaactatgg attaaagaac agaaaaagca
     1021 aggctgaaat tacctttcac aagtataagt tttcgtacaa atagcttatg tttgcagcat
     1081 aacattttaa gtttttctgc tgtatgaata tgaagcaact aatcgagaat tttccttcgc
     1141 aaatgcgcga ggctatgact attggcgaaa catttaaagc tacaagctgg ccacatgaaa
     1201 taaaaaatgt attggtgacc ggacttggtg gatctggaat tggaggaacc attgtttcac
     1261 aattgtgtga gaaagagatg ccgatacctt ttatggtgaa caaagattat tttatgcctg
     1321 catttgtaaa caaacattcg ctggtagttg taagttccta ttcaggaaat acagaagaga
     1381 cacttcatgc catggatatg gcaataaaac aaggtgcaaa agttgtatgt gtcagctcag
     1441 gaggcaaaac agttgaatta gcaaaacaac atggattgga tcatatatta attccgggtg
     1501 gcatgccacc acgttcctgc tatggatatt catcaacaca acttttctat atactacatg
     1561 cttttaaact tataggtgat ggttttaaag cctcattgaa gaatgctgtt gatttgttat
     1621 atgcagaatc tgctaatatt aaaaatgcat cacataagct tgcatcagag ctgattggga
     1681 aaattccagt catttattca gcttccaata atgaaggtgt tgttgtgcgt ttcagacaac
     1741 aagtgcagga aaatggaaaa atgttgtgct ggcatcatgt ttttccggag atgacacaca
     1801 atgagcttgt aggatggaca caaccacata atgatattgc tgtagtgttg ttcagagata
     1861 aagatgattt tttcagaaca gcaaagcgaa tggatatttg taaagagata tttggtcgct
     1921 acacaccaca catttacgaa gtatggagta aaggtggttc accaatagaa aaggcaattt
     1981 atttgattca ctttggtgac tggacaagct ggtatcttag cgaaatcaga aatatagact
     2041 gtacagaagt gaaagttatt gactacctta aaagtgaaat ggccaaactg aattaattat
     2101 tctgtttgaa tctgtcattc atgaaaaaag aaaatttatt tatagcagta gagggaatag
     2161 atggtagtgg taaaagtaca caggtacaat tgctgaaagc caaccttgaa gaagccggtt
     2221 ataaagttta tgcaacaaat gaacccacga attttgaagc aggaaaactc attcgttcaa
     2281 tttttaatca tagagtaaat gccgatcagc ataccattgc agcattgttt gttgcagaca
     2341 gattgcagca tttgcttgat aaggaaaatg gaattctggc aatgcttgaa aaaggctata
     2401 cggtaatcac agatcgctat tatttttcgt cctatgccta tcatagtgcc tatgtagaaa
     2461 tggattgggt aatgtctatc aatgctaaat gcgcagcgtt gttaaaacct gacctgaata
     2521 tttttgtaga tgtagatcct gaaactgcat ttaaacgcat tattgctaca cgcacctcaa
     2581 cagaattgta cgaaaccaaa gaaaaccttc gtaaagtcta tgataattat cttaaagcat
     2641 tcaaattact aaagaataca gaaagcattg tttttgttaa tggcaaaaat gatgttcagc
     2701 aagtagcagc tgatattttc aaaaccgtaa aaaatttatt gcatacctaa aaaaattgat
     2761 tgttttttta gtttagtttt ttatcattgc tgaaatttta aatcgtttca aaatgaataa
     2821 gaagcatcca aaggcattgc cctacctgtt cctgtctgaa atgtgggagc gattcggttt
     2881 ctatctcatg cttggtattt ttttccttta catgacagac acacaacgtg gtggcatggc
     2941 aatggacaga aaagaagctt cagatatttt cggaacattc attgcattag tttacttgac
     3001 tccgtttgtt ggtggattaa ttgccgatag agttttagga tatcgtgttt caattacact
     3061 tggtggcatt cttatgggta taggatattg tggattggca atacccggca tgactgcatt
     3121 ttatgtttca ctattactca tcatcattgg taatggattt tttaaaccaa atatttcgac
     3181 cattcttggt aatgtgtata acgatccgga gtataagcca ctcaaagatt cgggatacaa
     3241 tattttttac atggggataa acatcggagc atttatctgt aattttttcg gctcttattt
     3301 gcgaaacaac ttcagttggg gtgctgcctt tgccgctgca gggattggaa tgtttgtagg
     3361 tgtcattatt ttttggattg gcaataagca ctatgcacat gccgatgttc gcaagcctgt
     3421 aaagcccgaa gatatgcctg tatcaaaaat attaggtata acattattac ctgcattaat
     3481 tgcagggtat attggctgga taattcctga aaatattttc ggtagcgatt caaccgatgc
     3541 atttatttta ggtgccctac ctgttgccgg atattatgta aatatacttt tgcgttcaga
     3601 taaagaggac agacggccac tagctgcatt gctggcagta tttgctgttg caatagtttt
     3661 ttgggctgtg tttaaacaaa atggaacagc actaactaca tgggctgaaa gttataccaa
     3721 tcgtgaagta ccacagagtg tagaacctgt tgctaaagtg cttggagtaa cacaaacagt
     3781 aaccttctca aaagactctg taccattaac cgatagtcag tttcgtgtgg cacgtgacga
     3841 aaataaaaaa gcaataaaaa catgggatta cccactttat tataagaatg ttcctgcaga
     3901 taaattacct aaagaaggtg aatcgctaac attaatttca acagaattag gtcaatcaat
     3961 taatccattt tgggttgtaa tattaacacc tgtgattgtt gctttctggg catttctcaa
     4021 aagaagaaac agcgagcctt ctacaacatg gaaaatattt tatggctttc tcattacatc
     4081 attgtcaaca ttggtaatgg tcgcagctac ttatgcatgt cataatggtt tagagaaaag
     4141 ttctatgtgg tggctcattg caagctatgg agtaataact gttggtgagc tttgtttaag
     4201 cccaatggca ttgtcattgg tatcaaaact aagtccacca cgacttacag cactgatgat
     4261 gggcggatgg tttttatcca catcaatagg aaataaattg agtggcgtgc tggcaagtat
     4321 gtgggatgaa tatgaacata aagcaaactt tttcttagtt aatttcacat tagtaatgat
     4381 aactacaata attattttac tgatgttaaa atggcttaat aaaattgtga aagagcatgg
     4441 agcgtaatag tatattattg tttttattct gaaagtttat ttgcatttta ttcttgaatt
     4501 agtacggcag ataaaaatgt ttaaactctt tttagtagag aggtttttta atttgaaaat
     4561 ttcaacccct cacatgataa tatcagacct tcacccaaaa catatattac ttgcacagat
     4621 tttgaagtat tataggtata attcaaaatc cgagtattat gagattacta tctactgctg
     4681 ttattttatt aggatgcctc tgtgcacaag ctcaaattag tatcacaaat aatgaaatgc
     4741 cttctgccgg tgatacagca cgctttacaa aagctgttgt aaatccattg ataaacttta
     4801 gtgcaacagg caccaatcac acttggagtt ttggtaacct ccgcaacaat ggacaagatt
     4861 taaaatctta tcaaaatgtt tcatctacag gatttattta ttctttgttt ttttctaatc
     4921 tgccatttaa tccaaacaga gcaaacgtaa cagaacctgg acaagcatta ccttcaaacc
     4981 ctttgttgac aattaccgat ccatataatt tctattatcg ttcaagcagt gtatataaac
     5041 aagtaggctt cggtgcagag attgcgaata ttcctatgcc tgttgccttc agtcagcatg
     5101 atattattta caacctgcca ttaaattata acaattcaga tacctctcat tcagcatgga
     5161 atatcggtct tcccggctta ggttattatg gacttacaca gacaagaatt aatcacgttg
     5221 atggttgggg aacgcttaca acaccacatg gaacattcaa caccttacgt gtaaaaacac
     5281 aattgtatat aaaagatact ataagtgttg acacattaaa tctgaatttt gctttaaaca
     5341 gaccaaagac aacacaatat aaatggttag ccaataatga aattattcct gtgctacaga
     5401 ttacaaccac agaaatttta gggttagaag tagtgacaga aattatatat cgtgatgatt
     5461 acaatacggt atcaccagga agtttaaatg cagcatactg tgccggcagt aatgtatcaa
     5521 ttccatatac agctacaggt agtttcaatg gttccggatt tttacaaact gctaatagtt
     5581 ttacagcaca gttaagtgat gccactggca gctttgcaaa tgctgtaaat attggaagtg
     5641 ttacttcaag agtttctggg aatattaatg cagtaattcc tgcaaatact cctgtaggaa
     5701 atggttacag aatcagaatt atatcttcaa gtcctgcatt aacaggagga gataatgggt
     5761 ttgatttaag aattgagaac aatgcagttg cttcaattgt taatcagggt ttatcggcat
     5821 tctgcagtgg caactctgct ttattacagt cagttaatgt tgattccgga tatacttatc
     5881 agtggcaact aaatggcaca gacatttccg gagctactac agataactat ttggcatcag
     5941 caacaggcaa ctatacatta aacattacca actcatgtgg tactattaca tctaatgctg
     6001 ctgcaattaa tgttttgcca atgccaacgg ttcaggttac tgcatcagga ccattaacat
     6061 tgtgtctagg taccacagtt acactttcta ctttatttga ttctacatat tcctatcagt
     6121 ggcaattgaa tggtgttgat atttccggtg caacaacaga aaattacact gctgcaagtg
     6181 gaggagatta cacagttgta gtgagtaata gttgtggtaa tgtaacatcg tctgttcaaa
     6241 cagtttcagt tcaatcagct ccagttgcag gaagtctcac tgcagcttcg tcaacaatat
     6301 gtcatggtga ttctgtacaa attaactatt ctttgcagtc tgcaggcatt acctggttgc
     6361 ataatggaaa tattctttca ggcattacta ccaacagtca ttttgcaaat tctgccggtg
     6421 tgtattctgt cgttgaaagt gacttatgtg gtagtgatac ttccgttgct gtaatactga
     6481 cagcggacag tttaccaact gctgtaataa ctgcttccgg ggcattgtca ttctgtgcag
     6541 gaggagatgt tgtgcttaca gcagcaaccg gtaataatta cgcatggcaa tggtatatga
     6601 atggaacgcc tttggcaact acgcagttaa taacagcaac acagagtgga aattatgaag
     6661 tggaaattac aaatgcttgt ggctctgtca tgtcaactct tgttgcggtg actgcattcc
     6721 cattacctgt agtaccgaca attacacttt ctaacgattc attattagcg acaacgactg
     6781 taggtactta tcaatggttt tacaacggca atgccattac cggtgctaca aatgactttt
     6841 atgttccgtc acaaaatgga aactacagct taatggttac agatgctaac ggttgttcat
     6901 ccatgtcagc agtgtataat atgacaacag ttggattgca aactatattt aaacaagcac
     6961 ataatgtgtt tccaaatccg tttaacgact atctgacaat tgaattacca cagacagaaa
     7021 atgcaattat tttgactgtt gaaggaaagc aattgtggag cggtacgttg catcagggta
     7081 agaatgtatt gaatatgcag caatttgtta gcggcatgta tattctgcaa ctgaacaatg
     7141 aaaatggaat cacaaactac aaattagtta agcgataaac attgtgttat ctgaacaaaa
     7201 ggcaatttgc tttttgttca gatttaactg ttatgatttg cggtatttaa ctcctctgac
     7261 cgcatttcaa cagtagtctg ttctgtttca ggagttgttt caatgtgctt aaaaattgtt
     7321 cggttaaaaa gaataattaa taaaacacct gcagtaattg ctgaatcggc aatattaaaa
     7381 acaggtctga aaaaaataaa ttcttctccg ccccaaagtg gaaaccattg cggataatgt
     7441 gtctgaataa tcgggaaata aaacatatcc actactttac catgtagcca tccggcatag
     7501 cctccggcat cgggcataaa ggttgcaaca ccaaattcgc tggaggaaaa aaggcggccg
     7561 taaaaaatac tgtcaataat attaccaact gcaccggcaa aaattaatgc catagctgtg
     7621 gtgtaaatgt gatgcgcttt tgttttatgc agatgccata cataccaggc taatgcaaac
     7681 atgaccccga tacggaaaag tgaaagaaat ttttttccga acaattccat gccaaaggcc
     7741 attccattat tttcagtaaa atgtatgata aaccaattgc ctgcaatatg gtattcctga
     7801 ccgagataca tattggtttt aatccagaat ttcaaacact gatctaaaat cagaattacg
     7861 gaaacaagag caatatattt tttcaattgt atggattgat ataaaatgag gattgccaaa
     7921 aatgttgaca atcctcgtat tgtgtcataa ataaatatta atcgcgatac tgttgtacct
     7981 tggcttcaat gctttgtgta gtgtgtggca cagcacgaag tctttctttt ggaatcaact
     8041 tgccggttac acgacaaagg ccatacgttt tgttttcgat acgtaccaat gcattttcaa
     8101 gttgctcaat aaacttacgt tgacgcgcag caagttgtcc ggcttcttct tttgctaaag
     8161 tgtcgctgcc atcttcaagc atcttaaatg atgaagcagt gtcgtcagta ccatgttcat
     8221 tgctgttgtt aagaaatgcc tgcaggttgt ttaactcaac ttttgcatca gtcaattttt
     8281 gaataatcaa ctcgcgaaac tcgtttaatt ctgcatcact gtagcgtgtt ttatccgggc
     8341 tgttttccaa ctttgtaggt ctgttgtcaa gcattgcaac cggtttttta agaatagaag
     8401 caacaggtgc atctggaaga acagggtctt cacgtaatgg cgtagtacgc ggaggagcag
     8461 ccggtttaac aattggaggg ttgctgcgtc tgcgtgaact ttctcttggc ttctctacgt
     8521 gtttaatcat agatgttgcc gatttgcttt tatcctcttt ttgtgatttt gcagttacac
     8581 caggctttat aattttaatt ggttttggct taacaaccat tgcaggttta acaacctttg
     8641 ctttagcaac aggttttgcc tttgctactt tttttgcaac tgctttgctt ttaattactt
     8701 tgctttttgc aacagccttc ttagttacag tctttgattt agcaggttta ccttttgcta
     8761 ctgctttttt agcaactact ttttttgcta caggcttact tttaacagca gcctttttta
     8821 ccactttaac aactttttta acagctgttt ttttattttt tggtgtagct ttttttgctg
     8881 cagccttttt tggtgcagct ttctttgcta caggcttttt cttcgcttta cttgccattt
     8941 ttaaatcctt tctatttcaa ttaatgtttt aactccttct tcaacctcta tttcatcggc
     9001 taccggtgta gctaatgttt ctacc
//
LOCUS       DAORQT010000045         8968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1068_length_8968_cov_7.065634, whole genome shotgun sequence.
ACCESSION   DAORQT010000045 DAORQT010000000
VERSION     DAORQT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1068_length_8968_cov_7.065634"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PK134_02905"
     CDS             complement(<1..429)
                     /locus_tag="PK134_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37829.1"
                     /translation="MKHIAKLLWLTFFIPLLSSTASAQDTEEETSRWQIGLTAGINIP
                     LSDFADKLDENFKSGYAMLGATSRLRTSYHPNSKLYIAADYVWFYNPSDAKTLLNSLV
                     ETNPSGPRYKTTTYPWKVNSLLLGLGTTLNFNEQTHATLRG"
     gene            complement(626..1072)
                     /locus_tag="PK134_02910"
     CDS             complement(626..1072)
                     /locus_tag="PK134_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB37830.1"
                     /translation="MKRIAEIALMSMCVLGVCKSTFVNAQSNIAERKYRVIAYKQGAN
                     EITSMSNETTVTPNMAIYVPNSFTPNGDGINETFGAYSEGVKDFTMQVYDRWGAMIFE
                     SNDISKRWDGMYKGQIAQQGSYVYKIQAKAINGRILTKKGTVNLIM"
     gene            1609..1986
                     /locus_tag="PK134_02915"
     CDS             1609..1986
                     /locus_tag="PK134_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37831.1"
                     /translation="MHTIEQLEEMLAEHKEWQGKINFYRAEIDRMKTDLASAVMADRN
                     QYNMPHVEHYQNQFILQRDILDIMRHDFKQHENKIEAHSSNPVTNLAAAHQSEREKLG
                     SFERYFKELRNEFQAFIHKEAFS"
     gene            2140..3942
                     /locus_tag="PK134_02920"
     CDS             2140..3942
                     /locus_tag="PK134_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37832.1"
                     /translation="MKKSIICLVLLFHLTVLLFVACRKNDSDDVIPNVQQTSEQIRTT
                     ISGYVLNEMGQALSGVTVSTNGYIATTNDKGIFLLSKVIVDKNRCVISFSKSGFTKRN
                     YVFRPTDSSGQYVKLMLLNNVPNKTVSGVNGGTVSLPNGATIEFQPNSFTTANGTAYT
                     GTVNIYLKHLSPDDTDFSMMMPGGDMLAKDTTGKIVSLYSFGMMMAELTSTNNLPLQL
                     AAGKPATISMPIAPSQILKAPATIPLWYFDETTSLWIEEGGAAKVGSAYTGQVKHFSF
                     WNCDFPGERATIKGRIIDCNGNPMPNVVVTLDNWFDMLTDNNGVYQSWVPANISYVLQ
                     VLLSHNSGVFSSAPVNITPIAPGQTYTVPDISLPCPSSITGTLQNCAGVNVAGYVAVL
                     NLNANLLNLIYTGSGQFSINVPSQIPIVIYVSSLNNFEHYDTLNAISNMGTIINLGSI
                     QLCTYANDTTNLITINGAGFNNQTFIFTNALFMTSDLPSDTSIYSHELELGGILQPYG
                     SCYYYGISLKDTLAGTQPIINSTQVDFQLQTPQVDYAINLLPGGIIELHSNALIPGDY
                     FEGTYSGQADIINLNTVTSTQGTVIGKFRVRKQF"
     gene            complement(4183..4359)
                     /locus_tag="PK134_02925"
     CDS             complement(4183..4359)
                     /locus_tag="PK134_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37833.1"
                     /translation="MMNNDPIITYDHTKEENSFRSHYFKKSFFGRKNTMLLFFAEELL
                     ILGFMAYLVYLVFV"
     gene            complement(4581..5207)
                     /locus_tag="PK134_02930"
     CDS             complement(4581..5207)
                     /locus_tag="PK134_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB37834.1"
                     /translation="MFTVNLTEAGKKFKQHWIFRKCTAAFSSGDVIGITGKNGSGKST
                     LLQVISGYVSPNEGKIEWHEHDKKIEADSVYRYFSIASPYLMLIEDFTLEEMIRYYFR
                     FKNLRPAFNISSIITESGLEAHRNKLLSEFSSGMKQRTKLLLAIASDTPLLLLDEPCT
                     NLDKQNIAWYQQFLRTHSDGRLIFICSNHIDDELFLCNKWLSVEDFKI"
     gene            complement(5230..6027)
                     /gene="lpxA"
                     /locus_tag="PK134_02935"
     CDS             complement(5230..6027)
                     /gene="lpxA"
                     /locus_tag="PK134_02935"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRB37835.1"
                     /translation="MISPLAVVSPKAKIGNNVTIEPFTMVYDDVVIGDNTWIASNVTI
                     YDGARIGSNCRIFPGAVISAIPQDLKYKGEQTTAEIGDNTTIREFVTMNKGTTDKMKT
                     TLGSNCLVMAYAHIAHDCQLGNHIIMANNVTLAGHVLIDDWAILEGLVAVQQFVHIGA
                     HSFIAGGSLVRKNVPPFTKAAREPLSYIGINNIGLRRRGFSNEDVNHIQDIYRILFVQ
                     NSNVKKALAEIEKEIPESALRSQIVEFIHNSPKGLMRGFTPSDSDDE"
     gene            complement(6024..7406)
                     /locus_tag="PK134_02940"
                     /pseudo
     CDS             complement(6024..7406)
                     /locus_tag="PK134_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633900.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
     gene            complement(7403..8452)
                     /gene="lpxD"
                     /locus_tag="PK134_02945"
     CDS             complement(7403..8452)
                     /gene="lpxD"
                     /locus_tag="PK134_02945"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRB37836.1"
                     /translation="MKFTAKQIADLLKGTVEGNHDAEVNALSKIEEGKPGTLSFLSNP
                     KYTPYIYDTKASVVIVNKDFVAENAISATLIRVEDAYKSFASLLEIYNQIVNDKKGVE
                     SPSFIDPSATVGDQGYIGAFAYIGKNAVIGKNVKIYPHCYIGDHVIIGDNTTLFPGVR
                     VYSFCQIGMHCNIHAGVIIGADGFGFAPNENNNYKKVPQIGNVVIHDHVEIGAGTTID
                     RATLGSTVIHKGVKLDNLIQIAHNVEIGENTVIAAQTGVAGSTKIGKNCMIGGQVGII
                     GHLSIADGVKIAAQSGIGTSITTPNEIVQGSPAFNISDYKRTYVVFKKLPQLERKINE
                     LEKKTGSLNPAENKS"
     gene            complement(8632..>8968)
                     /locus_tag="PK134_02950"
     CDS             complement(8632..>8968)
                     /locus_tag="PK134_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37837.1"
                     /translation="TYDENLKAGKKFYINHFFLEGRIPKFYNNNVFPVDCTAAAQSLL
                     TLTRFNEQTLAFKVAEYMLEKMQHQKGYFYFRKYKNYTEKTSFMRWSNAWMFAGLTAV
                     LQSLSANRQ"
BASE COUNT         2749 a         1627 c         1625 g         2967 t
ORIGIN      
        1 ccctcttaat gtggcatgtg tttgttcgtt aaaatttaat gtcgtgccaa gtcctaaaag
       61 caaactgttg actttccatg gataagtagt tgtcttgtat cgtggtcccg aagggtttgt
      121 ttcaacaaga ctgttcagca atgtttttgc atcagaaggg ttgtagaacc acacataatc
      181 ggcagcaata tatagtttgc tgtttgggtg gtaagaagtt cgcagtcgcg aggtagcgcc
      241 aagcatggca tagccggatt taaagttctc atctaacttg tcggcaaaat cactcaatgg
      301 aatgtttatg cctgctgtta gacctatctg ccagcgtgat gtttcttctt cagtatcctg
      361 tgccgaggct gttgacgata aaagaggaat aaaaaaagta agccataata attttgcgat
      421 atgcttcatg tagcccgaaa gtttgcccta aagtagcaac tttacaggaa tcacaaacca
      481 agcaaggcaa ttttagtttt cagcttatgt atttgcgaaa ctactattcc ctttttgtcg
      541 aagtgcctga tgagatattt tagtagtatg attgacaatg ttctttgttt ttatagtgaa
      601 acattatgat ctgccttctt tgatattaca taataaggtt tacggttccc ttcttagtca
      661 atattctgcc gtttatagct tttgcctgta ttttatatac atagcttccc tgttgggcta
      721 tctggccttt atacatacca tcccagcgct tgctgatgtc attagattca aaaatcatgg
      781 ctccccagcg gtcataaacc tgcatggtaa agtctttaac accttcgcta taagcaccaa
      841 atgtttcgtt tataccatcg ccattaggag taaaggagtt aggcacataa atagccatat
      901 taggtgtcac cgtagtttcg ttagacatac tggtgatttc attggctccc tgcttgtagg
      961 caatgaccct gtacttgcgc tctgcaatgt tgctttgtgc atttacaaag gtggatttac
     1021 acacacccaa cacacacata gacattaagg caatttctgc aattcttttc atattattta
     1081 ttatttgtac agtgaattgc aaattgcttt cccgaatgtg gtttaattgt ttgaaaagcg
     1141 cagctaaatc acatatgtaa gattttatca tcctgagtta aagatttaag cacgtttttg
     1201 tgaattctcc tctgagaaaa atatatctga atcggaaaga cttgtgcttt ttatgtgaaa
     1261 gaaaaacgat ttcagcagcc attataaata caattagacc atgtatagca tattaaaaac
     1321 aagtccttcc gatattgaag attcttattc tgttgataag cctaaaaaac acatgaatct
     1381 gccaacgttt tgagaagttt ttgaacaata tgcttgttta atgataaaaa gtattacttt
     1441 taggaaaaat ttagtctgct ttagaatgaa aaattatagt tacagtattt atttttcatc
     1501 attattcttt tcaggcaaga tatttttctt tcttgtggct tctcattcca aagaagtgtg
     1561 ttgtatagat aaaccgccat tatatcgttc actctaaatc ttaataacat gcacaccatt
     1621 gaacaacttg aggaaatgct tgccgaacac aaagagtggc aaggtaaaat taacttctat
     1681 cgtgcagaga tagacaggat gaaaaccgat cttgcctcag cagtgatggc cgatagaaat
     1741 cagtacaaca tgccacatgt tgagcattac cagaatcaat ttattctgca gcgcgacata
     1801 ttagatatta tgcgtcatga ttttaaacag catgaaaata aaattgaagc acattcttca
     1861 aatccggtga caaatcttgc agctgcacat cagagtgagc gcgaaaagct tggttctttc
     1921 gaaagatatt ttaaagagtt gcgcaacgag tttcaagctt ttattcataa agaagctttt
     1981 agctgagagg caacgaagta tagatttgtg tagtcaagta attattcctg ctgattttgt
     2041 ttaaaagaat caggaaatag tgaaagtgtt atatttattg aatggagttg ttcatcaata
     2101 aaagctatgt tacattttac ttagattaaa atgatagtca tgaaaaaatc aattatctgt
     2161 ttagtgttat tgtttcattt aacggtttta ctatttgtag cctgtcgcaa aaatgattcg
     2221 gatgatgtta ttcctaatgt tcaacaaacg tcagaacaaa ttcgcaccac tattagtgga
     2281 tatgtactaa atgaaatggg gcaagcactt agtggggtaa ctgtttctac aaatggttat
     2341 atagcaacta caaatgataa aggaattttt ttactaagta aagttattgt tgacaaaaac
     2401 cgttgtgtta ttagtttttc taaatcaggt tttactaaac gaaactatgt ttttcgtcca
     2461 acagattcaa gcggacagta cgttaaattg atgttgttaa ataacgtgcc taacaaaaca
     2521 gtttcaggag ttaatggagg aactgtttca ctacctaatg gtgcaacaat agaatttcaa
     2581 ccaaattctt ttaccactgc taatggaact gcttatacag gaacagttaa tatatatctg
     2641 aaacatcttt cacctgatga tacggacttt agtatgatga tgcctggtgg agatatgctt
     2701 gcaaaagata caactggtaa gattgtaagt ttgtattcat ttggtatgat gatggcagaa
     2761 cttacatcaa ccaataattt gcctttacaa cttgctgcag gtaaaccggc aacaatctca
     2821 atgcctattg ctccttcaca aatattaaag gcccctgcta ctattccatt atggtatttt
     2881 gatgaaacaa catccttgtg gattgaagaa ggaggtgcag caaaggtagg gagtgcctat
     2941 accgggcaag ttaaacattt cagtttttgg aactgcgatt ttccaggtga aagagctact
     3001 attaaaggaa ggataattga ttgtaacggt aatcccatgc caaatgttgt ggtaacatta
     3061 gataattggt ttgatatgtt aacagataat aatggtgtct atcaaagttg ggtacctgca
     3121 aatataagtt acgtgttgca agttttatta agtcataact caggagtctt ttcaagtgca
     3181 cctgtcaaca tcacaccaat tgctcctggc cagacttata cagtacctga tatttcattg
     3241 ccttgtcctt cttcaattac aggaacattg cagaattgcg caggagtaaa tgttgcggga
     3301 tatgttgccg tattaaattt aaatgctaat ttattaaatt taatttatac aggcagtgga
     3361 caattttcaa ttaatgtgcc atcacaaata ccaatagtta tatatgtctc ttcactgaac
     3421 aactttgaac attacgatac attaaatgca atcagtaata tggggacaat aataaattta
     3481 ggttcaattc aactttgtac ttatgctaat gacactacaa atttaattac aataaatggt
     3541 gcaggattta ataatcagac ttttattttt actaatgctt tatttatgac ttctgattta
     3601 ccttcagata cctcaatata ttctcatgaa cttgagttgg ggggaattct ccaaccttat
     3661 ggcagttgtt attattatgg tatcagttta aaagacactc ttgcaggtac acaacctata
     3721 atcaattcaa ctcaagttga ttttcaatta caaactcctc aagttgatta tgcaataaat
     3781 ttattacctg gcggaataat agaattacat tcgaacgcat taattccagg tgattacttc
     3841 gaaggaacct attccggtca agccgatata ataaatttga atacagtcac aagtacacag
     3901 ggaacggtta taggaaagtt tagagtacga aaacaatttt gattcgtata agaaataaaa
     3961 attcgagata aattgttata atgaaagaaa taaaacaatg tagccgttac acttttttga
     4021 gtaacggcta ctatttatat atttatattc taaaactttt tacttttttt tctaaaaagc
     4081 tttgcctgat tcgttgttat tttttatcag taaaaacaaa tttactacat agcataacag
     4141 attttcaatt gttccagaag tcttaatttc caaaacaaat ttttacacaa acaccaaata
     4201 gactaagtag gccataaagc caagaatcag caattcttca gcaaaaaaca gcagcattgt
     4261 attttttcgt ccaaaaaaag atttcttaaa gtaatgcgag cggaatgagt tttcttcctt
     4321 ggtgtgatca taggtgataa ttggatcgtt gttcatcata gtcataagaa tttgcaggca
     4381 atttatatct gcactttgcc ggaatgggat aaaaaatgaa tttctgttta agatgtaaga
     4441 aattcagaaa agtaattggt ttttttgata cccttttttt gtgacgaaca gatttttcag
     4501 agagaaagat gttttaaaga aaaaaaattc aatctttaga acgcatgaaa gaataaacga
     4561 tacaagctat gttttgcagt tcagattttg aaatcttcaa cggaaagcca cttattgcac
     4621 aaaaacaatt cgtcatcaat gtggtttgaa cagataaaaa tcaatctgcc atctgaatgt
     4681 gttcttaaaa attgctgata ccatgcaatg ttttgtttgt cgaggttggt gcaaggttca
     4741 tctaaaagta aaagtggcgt gtctgatgca atggcaagca gtaacttggt tcgctgtttc
     4801 atgccagaag aaaattcact caacaattta tttctatgtg cttccaggcc ggattcagta
     4861 atgatagaag aaatattaaa tgctggccgt aggtttttaa aacggaaata atagcggatc
     4921 atttcctcta aagtaaaatc ttcaataagc attaagtagg gtgaggctat tgaaaaataa
     4981 cgatagacag agtcggcctc tattttttta tcatgttcat gccactcaat ttttccttca
     5041 ttaggtgata catagcccga aataacctga agtaaagttg atttgccaga gccgtttttg
     5101 cctgttatgc caataacatc accagaagaa aaagcagcag tacattttct gaaaatccag
     5161 tgttgtttga atttttttcc ggcttcagtt aagttaacag taaacacgat gtgatttttt
     5221 gttgttcaat tattcatcat cgctgtctga tggagtaaat ccacgcatca aacctttagg
     5281 cgaattatga ataaattcaa caatctggct tcgcaatgca ctttcaggaa tttctttttc
     5341 aatttcggca agtgcttttt taacgttact gttctgaaca aacaatatgc gatagatatc
     5401 ctgaatgtga tttacatctt cattagaaaa acctctgcgt ctgagtccga tattgtttat
     5461 gccaatatat gaaagtggtt cgcgtgccgc cttagtaaat ggcggaacat ttttccgcac
     5521 taacgatcct cctgcaataa aactatgtgc accaatatgt acaaactgtt gcactgcaac
     5581 caagccttct aatattgccc agtcatcaat taatacatgt cctgcaagcg taacgttgtt
     5641 tgccatgata atatgatttc ccaactgaca atcgtgtgca atgtgtgcat aagccatcac
     5701 taagcagtta ctgcccaatg tggttttcat tttgtctgtt gttcctttat tcatggtaac
     5761 aaactcacga atggtagtgt tgtcaccaat ttctgctgtt gtctgttcgc ctttatattt
     5821 taaatcctga ggaatggcag aaattactgc accaggaaaa atgcgacagt tgctgccgat
     5881 tcttgcacca tcataaatcg ttacgtttga agctatccat gtgttgtcac cgataacaac
     5941 atcatcgtaa accatggtaa aaggttcaat ggttacattg ttaccgattt ttgctttagg
     6001 gcttactact gcaagaggtg aaatcatgat ttttttacaa tttgagccat catttctgct
     6061 tccataacaa ctttgtcgcc tacaaatgcc tgtccgccca tgtggcaaag gccacgtctg
     6121 ataggtgaaa ttaaatcaag cttaaagaca acagtatcgc ccggcttaac catattctta
     6181 aaacgtgctg attctatttt taaaaagtat gtccagtaat ttccaggatc aggaacagta
     6241 ttgagtacaa gaataccacc tgtttgtgcc atagcttcaa ttagcaacac accgggcatt
     6301 acaggattac ccggaaagtg gccctggaaa aaccattcgt tggcagttac attttttaca
     6361 cctacaacac ctgtttcagt cagttcagtg attttgtcaa tcatcaaaaa cggaaaacga
     6421 tgtggcagga aagattcaat tctgctaatg tcgaatagtg caggtttatt tgcatcaatg
     6481 atgggtgcct ttaaacggtt tttatctttt ctgataattt ctttaaactg ttttgcgaaa
     6541 gcaacgttgg cagcatgccc cggacgcgca gcaagaatat gtcctttcag atgcacacca
     6601 agtaatgcaa agtcaccaac aatatctaac agtttatgcc gtgcaggctc attctgaata
     6661 ttatttagaa tgcctttatg ttatatctca acttttgctt tcttgaataa ggttgccaac
     6721 tcgtctaact tatcctgttt tatttcgcgg tcaacaatta caattgcatt attcaggtcg
     6781 ccacctttta ttaaatcatg tttaagcagc atttcaagtt catgcaggaa tacaaatgtt
     6841 ctgcattttg aaatctcatc tttaaactca gtaattttat gcatttgtgc atgttgtgtt
     6901 cccaacacag gtgagttgta gtcaaccatt accgtaatac gatagtcggg gcttggcaca
     6961 gcaagcattt caacattgcg ggcaggctct tcataggtga ggttttctgt aataacaaaa
     7021 taactacgtg ttgcttcttg ctcacgcaag ccggcctttt gaatggcatt tacaaatgga
     7081 aacgcactgc cgtcaagaat aggtacttca gggccatcaa tttcaataag cacattatct
     7141 acttccatgc ctaccaatgc agctaaaaca tgttctgttg tactcacctt tacaccgttt
     7201 gcacttaaag ttgttccgcg tgacgtatcc acaacattgt ccacatcggc atcaataatt
     7261 ggttggccgt ttacatcagt tcttttaaac ttgtatccat gattttcagg tgcaggcttc
     7321 aatgtaagtc tggcatttac accggtgtgc aaccccggac cttctacagt tatttcttct
     7381 tttaaagtaa cttgttttac actcatgatt tattttcagc agggtttaga ctacctgtct
     7441 ttttttctaa ctcgttaatt tttctttcta attgtggtaa ctttttaaac acaacgtagg
     7501 tgcgtttgta gtcgcttatg ttaaatgcag gagaaccttg aacaatttcg ttaggtgttg
     7561 tgatgctggt tccgatgccg gattgtgcag caatttttac gccatcagca atagataagt
     7621 gtcctatgat acctacctgt ccgccaatca tacagttctt tcctattttg gttgaacctg
     7681 ccactcctgt ttgtgcagca ataactgtgt tttcgccaat ttctacatta tgtgcaattt
     7741 gtatcaggtt gtctaactta acacctttgt gtatgacagt tgagcctaat gtagcacgat
     7801 caatagttgt tcctgcacca atttctacat ggtcgtgaat aacgacatta cctatctgtg
     7861 gaactttttt atagttgttg ttttcgttag gagcaaatcc aaaaccatct gcaccaataa
     7921 ttacacctgc atggatattg caatgcatgc ctatctgaca aaaagaataa actctaacac
     7981 cgggaaataa agttgtgttg tcaccgataa ttacatgatc accaatatag caatgcggat
     8041 aaatttttac gtttttacca ataactgcat tcttgccaat atatgcaaaa gcaccaatgt
     8101 aaccttgatc tccgactgtt gcagaagggt caataaaaga aggcgactca acaccttttt
     8161 tatcgttaac aatctgattg tatatttcaa gcagcgaggc gaagctttta taggcatctt
     8221 caactctgat gagtgttgca ctaattgcat tttcagccac aaaatcttta tttactatga
     8281 caacagatgc cttggtgtcg taaatgtatg gtgtgtattt tgggtttgat aaaaaggata
     8341 aagttccggg cttgccttct tcgattttag acaaagcatt tacttcggca tcgtgatttc
     8401 cctctacagt gcctttgagg aggtctgcta tttgtttggc ggtaaacttc atttgttact
     8461 caataaattc cggacttggt gcaatgtgtg caaatgtaca aatgagactg tgatatacaa
     8521 actcaaccaa taaatactat tcaactaaaa aataatgcta aaacagtggt tggcaagggt
     8581 ataaccaagc catggatttg ttaatgctga aaactgctat ggacaaatgc cttattgcct
     8641 attggcgctc aggctttgta aaacagcagt cagtccggca aacatccagg cattactcca
     8701 acgcataaaa gatgtctttt ctgtataatt tttatatttt ctgaaataaa aataaccttt
     8761 ttgatgctgc attttttcca gcatgtattc agcaacttta aatgcaaggg tctgttcgtt
     8821 aaatcgtgtt aatgtcagaa gtgattgtgc tgccgctgta cagtcaaccg gaaaaacatt
     8881 gttgttgtag aatttcggga tacgaccttc aagaaaaaag tggttgatgt aaaatttttt
     8941 tcctgctttt aggttttcat cataagtc
//
LOCUS       DAORQT010000046         8960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1071_length_8960_cov_8.676474, whole genome shotgun sequence.
ACCESSION   DAORQT010000046 DAORQT010000000
VERSION     DAORQT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_8960_cov_8.676474"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK134_02955"
     CDS             <1..534
                     /locus_tag="PK134_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB37838.1"
                     /translation="DKGKVVVNDFYQTNIPGIYAIGDIVKGQALAHVASAEGIICVEK
                     IAGHHPQPLDYNNIPGCTYCTPEIASVGMTEKAAKDAGIGIKVGKFPFSASGKAKAGG
                     QSDGFVKLIFDEKYGELLGAHLIGANVTEMIAELVLARKLETTGHEIIKAVHPHPTMS
                     EAIMEAAAAAYNEVIHL"
     gene            683..4048
                     /locus_tag="PK134_02960"
     CDS             683..4048
                     /locus_tag="PK134_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRB37839.1"
                     /translation="MQESKIYTPKHKIRIVTAASLFDGHDAAINIMRRIIQSSGAEVI
                     HLGHDRSAQEVVECAIQEDANAIAMTSYQGGHMEYFKYMYDLLKERGCGHIKIFGGGG
                     GTILPEEISELQDYGIARIYHPDDGRAMGLQGMINDMLQKCDFATGVNLNGEIKELTK
                     KNSKAIARLISCAENNQSEFEKLYPEITSSLSNKTKNSPVLGITGTGGAGKSSIVDEL
                     VRRFLLEFKDKYIAIISIDPSKRKTGGALLGDRIRMNSIRNPRVYMRSLATRQSNLAL
                     SRYVQEALDIVKAAQFDLIILETSGIGQSDTEITEHSNVSLYVMTPEYGAATQLEKID
                     MLDFADLIALNKFDKRGALDALRDVKKQYKRNHKLFDIGDEKIPVFGTIASQFNDPGM
                     NQLYKSVMNTIAAKTGADLKSDYHTTEEMSEKVFIIPPHRTRYLSEISENNRHYDQWV
                     EEQCTLAQKLYSLYESMQIIKGAAAEELKKQYDILERSFDGNNKKLLEDFPAKTEEYS
                     KDYYEFKVRDKVLKIKTFDESLSHNKIPKVAFPKYKAWGDLLKWILQENVPGEFPFTA
                     GVFRFKREGEDPTRMFAGEGCPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGNDPDHR
                     PDIYGKIGNSGVSVCCLDDAKKLYSGFNLCDPATSVSMTINGPASIIAAYFMNAAIDQ
                     QCEIYIRQNGLEKEVEKKIADEFSKNKSSQPKYNYEIPEGNNSLGLMLLGITGDKVLP
                     KDVYDKIKTDTLKQVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDMQQYFIDKAIRN
                     FYSVSISGYHIAEAGANPISQLAFTLSNGFTYVEYYLSRGMDINSFAPNLSFFFSNGI
                     DPEYSVIGRVARRIWAKAMKLKYKANERSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQ
                     ALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGLAKNENPLQGSFII
                     EELTDLVEEAVLSEFDRITERGGVLGAMETMYQRGKIQEESLYYESLKHTGAYPIIGV
                     NTFLSSKGSPTILPKEVIRATEEEKQQQIATLKNLHARYASTAPEMLHDLQLTAIHNQ
                     NMFEKLMEVSKYCSLGQITNALFEVGGQYRRNM"
     gene            4387..4848
                     /gene="tnpA"
                     /locus_tag="PK134_02965"
     CDS             4387..4848
                     /gene="tnpA"
                     /locus_tag="PK134_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRB37840.1"
                     /translation="MMSTYSQIYIQIVFAVKGRESLILPQWEERLHKYITGIVQNKEQ
                     KMLAINGMSDHLHFLIGMKPSCCLSDLVREIKKSSNDFIKVEKLSKFKFSWQEGYGAF
                     SYSHSQLNDVIAYITNQKEHHRKKSFKEEYVEFLKKFNVDYNEKYLFEWME"
     gene            4941..5714
                     /locus_tag="PK134_02970"
                     /pseudo
     CDS             4941..5714
                     /locus_tag="PK134_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745327.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(5772..6791)
                     /locus_tag="PK134_02975"
     CDS             complement(5772..6791)
                     /locus_tag="PK134_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRB37841.1"
                     /translation="MKLVTYQNQNKTALGILLSDKVYNLSELSQRRGKTVLPDNMRDF
                     LTGEQLMMDAAMELNQWLSDGNADADGLSVANVNLLAPVPNPTSCRDGYAFRQHVEAA
                     RRNRRVEMIPEFDQYPIFYFTNHNAIQGPGDIFCMPDHFEQLDFELEAAIVIGRKGIN
                     VKAKDADSYIAGYMIMNDMSARKLQMEEMKLNLGPAKGKDFSTVIGPYLVTPDELASY
                     KTIAKSGHTGNNYNLKMTCKVNDIQVSEGNVGSMDWTFAEIIERCAYGVNILPGDVIG
                     SGTVGTGCFLELNGTGLFNNPDYKPQWLKEGDKVEMEIAGLGVLTNTIRKIDSDFSIL
                     ALKKM"
     gene            6938..7453
                     /locus_tag="PK134_02980"
     CDS             6938..7453
                     /locus_tag="PK134_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37842.1"
                     /translation="MIRKILATQALLLCSASFLFAQHTLVLKSGEKMTGTVQQIKDGN
                     ISFLFKGNPMNFKVSEASAIFFDAASETKADNSEMSADMRGVKYEMPGRTITKPPKIE
                     NLTMEKGVVVVEISIDKYGHVTKANPGAEGTTTTSSYLLTKAKQAAESVMFNNCPKCP
                     LDTKGTITLTF"
     gene            7480..8379
                     /gene="miaA"
                     /locus_tag="PK134_02985"
     CDS             7480..8379
                     /gene="miaA"
                     /locus_tag="PK134_02985"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRB37843.1"
                     /translation="MPKDKKLIIIAGPTASGKTQIAIEVAKLHSTEIVSFDSRQIFRE
                     MTIGTAVPDAQQMNTVKHHFIHSHSIAEDMNASRYADEATNTINQLLEKNETVVLTGG
                     TGMYMHAALFGLDPTPNTDYQLRASLEKECAEKGLEYLAHQLKTLDEKSYHQIDIHNP
                     RRVIRALEIIYSSGKTRSSFIEDKNKISTYNFTLYVLNPDRQLLYDNINQRVDMMIEK
                     GLVDEAKLLLPYRHLTALHTVGYKELFEYFDRNITLDQAIEKIKQHTRNYAKRQLTWF
                     RQYDDAVWITPEEMKKKIEKKNL"
     gene            complement(8376..>8706)
                     /locus_tag="PK134_02990"
     CDS             complement(8376..>8706)
                     /locus_tag="PK134_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37844.1"
                     /translation="YTFSADTKAGDKVYKETGEFSVVPLLLENFESTANHQLLNTISS
                     NTGGIMVTPDKINLLIDAVQKNETVKPVSYYHKKLIELISLKSLLAICLLLIAMEWFL
                     RKRFGSY"
     assembly_gap    8707..8718
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8719..8910)
                     /locus_tag="PK134_02995"
     CDS             complement(<8719..8910)
                     /locus_tag="PK134_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37845.1"
                     /translation="MIQYKRSYTEGENIEFDATLYNAAGELINEPEVRLNIYNDEKKE
                     FRYTFNRSNNAYQLNAGMLP"
BASE COUNT         3016 a         1699 c         1694 g         2539 t
ORIGIN      
        1 gataaaggta aggttgttgt aaacgatttt tatcagacca acatacctgg catttacgca
       61 ataggtgata ttgtaaaagg tcaggcattg gcacatgtgg cttctgcaga aggaattatt
      121 tgtgttgaaa aaattgcagg ccatcatcca caaccactcg attacaacaa tattccggga
      181 tgtacttatt gcactcctga aattgcttct gtaggtatga cagaaaaagc tgctaaagat
      241 gcaggcatag gaataaaagt tggtaagttc ccattcagtg cttcaggaaa agctaaagcc
      301 ggtggacaaa gtgatggttt cgttaagctt atttttgacg aaaaatatgg tgagttgctt
      361 ggtgcacatt taataggtgc aaacgttact gaaatgatag cagagttggt tctggcacga
      421 aaattagaaa caacaggtca tgaaattatc aaggctgtac atccgcatcc tacaatgagc
      481 gaggctatta tggaagctgc tgctgccgcc tataatgaag tgatacattt ataggattgc
      541 cctttgcttg ttgtaaccct acaacaattt cgcaattttt aattagctat aggtgtgttt
      601 ttcattctta taagataact acacctcccc tttcctgctt acgctgtaaa tgttaaattt
      661 gcagcataaa ggttattctg atatgcagga gtcaaaaatt tataccccga aacataaaat
      721 ccgtattgta actgccgctt cgctgtttga tgggcacgat gctgccatca acatcatgcg
      781 cagaatcatt cagtcaagtg gtgctgaggt tattcacctt ggccacgaca gaagtgcaca
      841 agaggtagtg gaatgtgcca ttcaggaaga tgccaatgct attgccatga caagttatca
      901 gggcggacac atggaatatt ttaagtacat gtatgacttg ctgaaagaaa gaggctgtgg
      961 gcatataaaa atatttggtg gcggtggtgg cacaatactt ccagaagaaa taagcgaact
     1021 tcaggactat ggtattgccc gcatctatca tcctgatgac ggacgtgcta tgggcttgca
     1081 aggcatgatt aacgatatgc tccaaaaatg cgactttgcc acaggagtaa atttaaatgg
     1141 cgaaataaaa gagctgacaa agaaaaattc aaaagccatt gcaaggctca tttcttgtgc
     1201 agaaaataat caatcggaat ttgaaaaact atatcctgaa attacttctt ccctctctaa
     1261 taaaaccaaa aacagtcctg tattaggaat tacaggaaca ggaggcgcag gcaaatcatc
     1321 tattgttgat gaattggtaa gaagattttt gttggagttt aaagataaat atattgccat
     1381 catttccatt gaccccagta aacgcaaaac aggtggtgca ttacttggcg accgtatcag
     1441 aatgaacagt attcgcaatc cacgtgtgta tatgcgctcg ttggcaactc gtcaaagtaa
     1501 tcttgcattg agtcgttatg ttcaggaagc attagatatt gtaaaagccg cacagtttga
     1561 tttaattatc cttgaaacat caggtattgg acaaagcgat actgagatta ccgaacatag
     1621 caatgtgagt ttatatgtaa tgactccgga atatggtgca gcaacacagt tggaaaaaat
     1681 tgacatgctt gattttgccg acctcattgc actaaacaaa tttgataaac gcggagcatt
     1741 agatgcattg cgcgatgtaa aaaaacaata caagcgcaat cataagttgt ttgacattgg
     1801 tgatgaaaaa attcctgtct tcggaacaat agcctctcag tttaacgatc cgggtatgaa
     1861 tcaactttat aagtctgtga tgaataccat tgcagcaaaa accggagcag acttaaaaag
     1921 cgactatcac accacagaag agatgagtga aaaagtgttt atcattcctc cgcatcgtac
     1981 acgctatctt tctgaaatct cagaaaacaa cagacattat gaccagtggg ttgaagaaca
     2041 atgtacactt gcacagaagc tttactcgct ctatgaaagc atgcaaataa taaaaggagc
     2101 agctgccgaa gagctgaaaa aacaatatga tatacttgaa agaagtttcg atggcaacaa
     2161 caaaaaacta ttggaagatt ttcctgctaa aacagaagag tacagtaaag attattatga
     2221 atttaaagta cgtgataaag ttttaaaaat aaaaacattt gatgaatcac tttcgcacaa
     2281 caaaatacct aaggtagctt ttccgaaata caaagcttgg ggcgatttgc tgaaatggat
     2341 tttgcaggaa aatgttcccg gtgaatttcc gtttactgca ggtgtattcc gttttaaacg
     2401 cgaaggcgaa gacccaacaa gaatgtttgc cggtgaagga tgtcctgagc gtaccaacaa
     2461 acgtttccat tatgtttcat taggcatgcc tgctaaacgg cttagcactg ccttcgatag
     2521 tgtgacactc tatggcaacg accctgacca tcgtcctgat atttatggga aaataggaaa
     2581 cagtggtgta agtgtatgtt gtcttgatga tgcaaaaaaa ttgtatagcg gatttaatct
     2641 ttgcgaccct gcaacttctg tatcaatgac cattaatggt ccggcttcaa ttattgcagc
     2701 ctattttatg aatgcagcta ttgatcagca atgcgaaatt tatatcagac aaaacggatt
     2761 agaaaaagaa gttgagaaaa aaattgctga cgaattttca aaaaataaat caagccaacc
     2821 aaaatacaac tacgaaattc cagaaggaaa caattcgctt ggccttatgc ttctgggaat
     2881 aaccggtgac aaagttcttc caaaagatgt ttacgataaa ataaaaaccg acacattaaa
     2941 gcaggttcgc ggaactgtac aagcagatat tctaaaagaa gatcaggcac aaaacacttg
     3001 catcttctct acagagtttt ctcttagatt gatgggcgac atgcaacaat attttattga
     3061 caaagccatt cgtaattttt attctgtttc cataagcggc taccacattg ccgaagccgg
     3121 tgccaatcca atttcgcaac ttgcttttac attgagtaat ggtttcactt atgtggaata
     3181 ttatttaagt cgcggcatgg acatcaattc ttttgcgcct aacctttcat tctttttctc
     3241 caatggcata gaccctgaat attctgttat cggacgtgta gcaaggcgta tttgggctaa
     3301 ggctatgaaa ctaaaataca aggccaatga acgttcgcag atgcttaaat atcatattca
     3361 aacatcgggc agaagtttgc atgctcagga aattgacttc aacgatatca gaacaacact
     3421 gcaggctctg tatgctattt atgacaactg caactcactt cacaccaatg cttatgatga
     3481 agccatcacc actcctacag aagaaagtgt gcgcagggca atggcaattc aacttatcat
     3541 caaccgcgaa ttgggtcttg ccaaaaacga aaatcctttg caaggttcat ttataattga
     3601 agagttaact gatttggtag aggaagcagt actgtcggag tttgaccgca ttacagagcg
     3661 tggtggtgtg ctgggtgcaa tggaaacaat gtatcagcgt ggaaaaattc aggaagaaag
     3721 tctttactac gaatcgttaa aacacaccgg tgcttatccg attattggtg tcaatacatt
     3781 tttatcgagc aaaggctcac caactatttt acctaaggaa gttattcgcg ccacagagga
     3841 agaaaagcaa cagcaaattg ctactttaaa aaatctgcat gcacgttatg catctactgc
     3901 acctgaaatg ctgcacgacc tgcaactcac tgcaatccat aatcaaaata tgtttgaaaa
     3961 gctgatggaa gtaagtaagt attgttcctt aggacaaatt accaatgcat tgtttgaagt
     4021 tggcggacag tataggagga atatgtaaat tacaatttag taattaatag taaaatcatt
     4081 attcgtgtca accaaaacag taacttaaat taatctaaaa cctaattaat ataagtgaat
     4141 caagacccca atgatgaacc tgctttagcc ttgttggagc ggataaagaa aatgaagaat
     4201 ggaaaaggga agtatggaaa gaatgaaggt taaataagaa agtctttatt taaccttaat
     4261 gactacaaac ttaaatagca aaacttttat ttaactttgg ctaaagatgc caaaggcatc
     4321 caacctttat agaaaaacaa aaccaacaaa caacacgact ccgaaggggt cgaacataaa
     4381 ccataaataa tgtcaaccta ctcgcaaatt tatattcaaa ttgtttttgc cgtaaaagga
     4441 agagaaagcc ttattctgcc acaatgggaa gagcgtttac ataaatacat tactggcatt
     4501 gttcaaaaca aagaacaaaa aatgcttgcc ataaacggca tgtccgacca tttgcatttt
     4561 cttatcggca tgaaaccatc ttgttgttta tccgatttgg taagagaaat aaaaaaatca
     4621 tcaaacgatt ttatcaaagt tgaaaagcta tcaaaattca agtttagttg gcaagaaggc
     4681 tacggtgctt tttcgtatag ccattcccag ttgaacgatg ttattgcata catcaccaat
     4741 caaaaggaac atcaccgaaa gaaatctttt aaagaagaat atgttgaatt tttaaagaag
     4801 tttaatgttg attacaatga aaaatatttg tttgaatgga tggaatagtt cgatgcctac
     4861 ggcatcgcgt tttacaaacc gaattaatgc tataaagcta taatcccttt ggggttattg
     4921 atgtgaaaac aacgaaatat atgagcaacg aaaatacatc agcaatagta agtaaggtat
     4981 ggagtttctg taatccgcta agagatattg gcgttggtta tggcgactac ttagaacaac
     5041 ttacctattt gctgtttttg aaaatgactt atgaattcag catctccccc ctacaacaga
     5101 aaactgccaa tcccaactga aaacaattgg gaaagtttga cttcaaggcg tggtgatgaa
     5161 ctggaataat atcataccaa aaccctaagt gttttgtcaa ggcaaaaagg agtgttgggg
     5221 caaatatttg ttcaaagtca gaataagatt aaagaacctg ccatgcttta caaaatcatt
     5281 gacatgattg ataaagagca gtgggcaaca ttgggagcag acataaaagg aaccatttat
     5341 gaaggtctat tagagaataa cccataagac acaaaaaggg tgcaggacaa tattttacgc
     5401 tgaaaaagaa tccgctgaaa ttggaagacc ttcgggactt cattacttgc tacaatcctg
     5461 acaaccgaca caaaagaaag gaaacttaca actcagacac aaatcctgaa ggtcgttgga
     5521 gaaagttcac ttatgatgaa attatcaacc gtgacaagac atcgcttgac atcacttgga
     5581 taaaagataa gtcacttgcc gaccttgaca acttacccga cccggatgtt ttggcaggag
     5641 aaataattga aaaccttgaa gctggactgg aaagtttcag agaaataatg attacaataa
     5701 atggaaagga ctaaaaatat cctcttgctg atagttccta aataaaaaca gcattcttct
     5761 ttttcaatct cctacatctt cttcaatgca agtattgaaa aatcagaatc tatttttctg
     5821 atggtatttg tcagcacacc aagaccggca atttccattt caactttatc tccttcttta
     5881 agccactgtg gtttatagtc aggattattg aataaccctg tgccgttcaa ctctaagaaa
     5941 catcctgtgc caacagtacc gctgccaatt acatcaccgg gaagaatgtt tacaccgtag
     6001 gcacaacgct caataatttc tgcaaatgtc caatccatac ttcctacatt accttccgac
     6061 acctgaatgt cattgacctt acaagtcatt tttaaattgt aattatttcc tgtgtgtccg
     6121 ctttttgcaa tggtcttata tgatgcaagt tcatcaggcg ttactaaata cggaccaata
     6181 acagtagaga aatcttttcc ttttgcaggg cctaagttga gtttcatctc ttccatctgc
     6241 aatttgcgcg cactcatatc gttcataatc atatagccgg caatgtagct atcggcatct
     6301 tttgctttta cattaatgcc tttgcgacca atgacaattg ctgcctcaag ttcgaagtca
     6361 agttgttcaa aatgatcggg catgcaaaaa atatctccag ggccttgtat agcattgtga
     6421 ttggtaaaat aaaaaatcgg atactgatca aattccggaa tcatctctac acgtctgttg
     6481 cgccttgctg cttcaacatg ttgcctgaac gcataaccat cacggcaaga agttggatta
     6541 ggcaccggtg ccaataaatt tacattagct actgagagtc cgtcagcatc tgcattgcca
     6601 tctgaaagcc attgattcaa ctccatagca gcatccatca ttagttgttc gcctgttaaa
     6661 aaatcacgca tattgtcggg caacacagtt tttcctctgc gttgtgacaa ttcgctaaga
     6721 ttataaactt tatcactaag caagattccc aatgccgttt tattctgatt ctgataggtt
     6781 actaatttca tattttacta ctttatggtg ccaaaattat cattctgtat cgaagatgcc
     6841 taatatagct tctattgttg aaaactgttt tcatatttca attcaaattc attcttagaa
     6901 cagctataaa aatttatttt tgaaaaaagt ttaaatcatg atcagaaaaa ttcttgcaac
     6961 acaggcgctt ttattatgca gtgctagttt cttatttgca caacataccc ttgttttgaa
     7021 aagtggcgaa aaaatgacag gcacagtgca gcagattaaa gatggcaata tcagcttttt
     7081 atttaaaggc aatcccatga attttaaagt gagtgaagcc tctgccatat tttttgatgc
     7141 agcatcagaa acaaaagcag acaactcaga gatgagtgcc gatatgcgtg gtgtaaaata
     7201 tgaaatgccc ggacgtacca tcaccaagcc gccaaagatt gaaaatctta ctatggaaaa
     7261 aggtgttgtt gtagttgaga tttctattga caaatatggc catgttacca aggctaatcc
     7321 aggtgcagaa ggcacaacca ctacaagcag ctatttgtta actaaagcaa aacaggcagc
     7381 agaaagtgta atgtttaata actgccctaa atgtccgtta gataccaaag gcactattac
     7441 acttaccttt taactttgcc ataagcaaag aataagaaat tgcctaagga taaaaagtta
     7501 attattattg cagggcctac agcttcgggc aaaacacaaa ttgccattga agttgctaaa
     7561 ctgcatagca cagaaattgt ttctttcgat tcgcgacaga tttttcgtga aatgactatc
     7621 ggcactgccg tgccagatgc ccaacaaatg aatacagtga aacatcactt cattcattcg
     7681 cattctattg cagaagatat gaatgcaagt cgctatgccg atgaagcaac taacaccatc
     7741 aatcagttgt tagaaaaaaa tgaaacagtt gttcttacag gcggcacagg tatgtatatg
     7801 catgctgctc tcttcggcct cgaccctact ccaaataccg attatcaact tagagcaagt
     7861 cttgaaaaag agtgtgctga aaaaggattg gagtatctgg ctcatcaatt aaaaactctt
     7921 gacgaaaaaa gctatcatca aatagacatt cacaatccac gcagggtcat tcgtgcatta
     7981 gaaattattt acagctcagg caaaacacgt tcatctttta ttgaagataa aaacaaaata
     8041 tctacttaca acttcacact ttatgtttta aatcccgaca ggcaattact ttatgataac
     8101 ataaatcaac gtgtggatat gatgattgaa aaaggattgg tggatgaagc gaaactactt
     8161 ctaccctacc ggcatcttac ggcattgcac actgttggct acaaagaatt atttgagtat
     8221 ttcgatagaa acattactct tgaccaagcc atcgaaaaaa taaaacaaca tacacgcaac
     8281 tatgccaaaa gacaattgac atggttcagg cagtatgatg atgccgtttg gattacaccg
     8341 gaagaaatga aaaagaaaat cgagaaaaaa aatctttaat aagaaccgaa acgttttctt
     8401 aagaaccact ccattgcaat taacaacaag cagatcgcaa gtaaactttt caggcttatc
     8461 aattcaataa gtttcttatg ataatacgaa acaggtttca ccgtttcgtt tttctgaaca
     8521 gcatcaatca acaaattgat tttatcggga gtcaccataa tgcctcctgt gtttgatgaa
     8581 atggtattga gcagttgatg attggcagtt gattcaaaat tctccagcaa caatggcaca
     8641 acagaaaact ctcctgtttc cttataaact ttatcgcccg cttttgtatc cgcactgaaa
     8701 gtatagnnnn nnnnnnnntg gcaacattcc cgcattcaac tgataggcat tgttgcttct
     8761 gttaaaagtg tatcggaact cttttttctc atcgttataa atattcaggc gcacctccgg
     8821 ttcattaatt aattctccgg ctgcattata aagcgtggca tcaaactcaa tattctcacc
     8881 ttctgtatag gagcgcttgt actgtatcat aaaaggctct tttttctctt ttgaagtcag
     8941 gtactgaaca gattttaaaa
//
LOCUS       DAORQT010000047         8923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1076_length_8923_cov_12.068336, whole genome shotgun sequence.
ACCESSION   DAORQT010000047 DAORQT010000000
VERSION     DAORQT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1076_length_8923_cov_12.068336"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1552
                     /gene="ffh"
                     /locus_tag="PK134_03000"
     CDS             221..1552
                     /gene="ffh"
                     /locus_tag="PK134_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRB37846.1"
                     /translation="MFENLSDKLEKAFKNLKGQGKITEINVAETLKEVRRALLDADVN
                     YKVAKQFTDTIKEKALGQNVLTAVSPGQLLVKIAHDELATLMGGQMTDIQLKGNPSVI
                     LMSGLQGSGKTTLSGKLANFLKTKKGKTPLLVACDVYRPAAIDQLKVLGEQIGVEVYS
                     EVENKNPVEIARHAIKQAKQNGNNVVVVDTAGRLAIDEQMMNEIAALKNAIEPDEILF
                     VVDSMTGQDAVNTAKAFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVTLVEKAQEQFDEKQAAELQKKFAKNQFNFNDFLS
                     QLQQIKKMGNIKDLVSMIPGVGKAVKDMDIDNNAFKSIEAIIQSMTPAERANPDLING
                     NRKKRIAAGSGTGIPEVNKLIKQFEDMRKMMKMMTTGNMKNMMRNMPGMRR"
     gene            1583..2464
                     /locus_tag="PK134_03005"
     CDS             1583..2464
                     /locus_tag="PK134_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRB37847.1"
                     /translation="MTIIDGKQTAEEYKSILKQEVKALVATGKRPPGLAILLIGNNGA
                     SETYVANKLKTCHDVGFNASLFRFENTVSENEILNCINELNFNSSIDGVIVQSPFPNH
                     ISPQKINEAIHPAKDVDGFHPMNAGRLQQNKPCYIAATPKGIMQLIEKYKIETQGKHC
                     VVVGRSNIVGMPMSILLSRNSKPGNCTVTICHSKTVDLAAYTKRADILIAAIGSPELI
                     TADMVKEGAVVIDVGITRVADKATKSGYRLKGDIKYDEVAPKCSFITPVPGGVGPMTI
                     IGLLQNTLLAAKNAIYN"
     gene            2476..4275
                     /gene="argS"
                     /locus_tag="PK134_03010"
     CDS             2476..4275
                     /gene="argS"
                     /locus_tag="PK134_03010"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRB37848.1"
                     /translation="MIFEQELASITNEALKVLFNFEPDESLLTYTQTRKEFDGDYTIV
                     VFPFVRQSKKSPEETGRLIGEYLIKHASFISNYNVIKGFLNITVSDEQWLKRIAVAFA
                     DSEYGFTKADKSKPVIVEYASPNTNKPIHLGHLRNILLGYSVSLLLKATGRNVKRVSI
                     INDRGVHICKSMLAWKKFGNDETPQSSGMKGDHLVGKYYVEFDKQYKQQVSEMISAGI
                     EKDKAEKNAPLMQEVRQMLIEWEQGNEEVTALWKKMNGWVYEGFDVTYNRLGVDFDKT
                     YYESETYLLGKKIVEEGLSKGVFYKKADNSVWVDLTNDGLDEKIVQRSDGTSVYITQD
                     LGTAVLRHDEFDFDSKIYVVGNEQDYHFKVLFLILKKLGYEWAEKLYHLSYAMVDLPS
                     GKMKSREGTVVDADDLMDEVTAAAAEETSKLGKVEEFSSDELKQLYETLGFGALKYFI
                     LKVDPEKRMLFNPAESVEIHGNTGPYIQYVHARICSVLKRASQMEQLSNYKNTAIESL
                     VSMNEKERELAINILRFKETVETAAEKFSPAIVANYVYELAKNFNQFYHDNSIISEGE
                     LETSQFRLCLADACAMTIKKSLNLLGIKAPERM"
     gene            complement(4294..4980)
                     /gene="cmk"
                     /locus_tag="PK134_03015"
     CDS             complement(4294..4980)
                     /gene="cmk"
                     /locus_tag="PK134_03015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRB37849.1"
                     /translation="MIIAIDGYSSCGKSTVAKAIAKRLKLNYIDSGAMYRAVTYYFLQ
                     NNIPFSKTADVIADYSSILENIQIEFIRNEQTGQSEIYLNGKNIEAEIRQMDVSDNVS
                     YVSAIKSVRKKLVAMQQQMSTLGNLVMDGRDIGTTVFPNADLKIFMTASPEVRVYRRY
                     HELIEKGIKVTPDEIRKNIELRDYEDTHREESPLRKPDDAIILDNTYMSRDEQVQFVL
                     DAVAKLKKAI"
     gene            complement(5055..6113)
                     /gene="porQ"
                     /locus_tag="PK134_03020"
     CDS             complement(5055..6113)
                     /gene="porQ"
                     /locus_tag="PK134_03020"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HRB37850.1"
                     /translation="MRITTLIAALLLPVCLKAQTGGSAIFRFLDLPSSARHAALGGNF
                     ISIRDNDLNTSFYNPAILNVDMDKQITFNYNPYFSGIKYGYTAYAHHIENIGTLSAGI
                     QYVSYGKFDRTDETATDLGTFSGGDYALNISMSRPVGIDSVLFVGASLKFIYSHIDTY
                     SAFGIAADLGVNYYSPDKLTSAALVIRNAGRQLKSYTEKNNEPLPIEIEAGITQKLAK
                     APFRFTLTYRHIEKWDLSYIDNNSQETDPLTGEVKEPEKASFGNKLLRHLIISNEILI
                     SKNFHLRMAYNFQRRNEMKVESRPAMVGISFGLGLKISKFHLSYGRSLYHLAGGTNSL
                     SISTYLNDFKRKPKTTDS"
     gene            complement(6178..7446)
                     /gene="glyA"
                     /locus_tag="PK134_03025"
     CDS             complement(6178..7446)
                     /gene="glyA"
                     /locus_tag="PK134_03025"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRB37851.1"
                     /translation="MQKDKAVFKLIKEELARQRHGLELIASENFVSQQVMDAMGSCLT
                     NKYAEGLPGKRYYGGCEVVDEIEQIAIDRAKTLFNATWANVQPHSGAQANAAVMLACL
                     QPGDTILGFNLSHGGHLTHGSPVNFSGKLYRPTFYGVEENTGTIDYNKVEETALKEKP
                     KLIICGASAYSRDWDYVALRSIADKVNALLLADISHPSGLIARGLLNDPLPHCHFIST
                     TTHKTLRGPRGGLIMMGKDFENPWGLKTPKGETRMMTALMDSAVFPGTQGGPLEHVIA
                     AKAVAFGEALSDEYFEYCLQVIRNAKTMANAFVKKGYKVISGGTDNHLMLIDLRSKNI
                     TGKQAENALVRADITVNKNMVPFDTQSPFVTSGMRVGTPAMTSRGMKETEMARIVELI
                     DMVIMNHTNGAVIEEVKTTVNDMMEHFPLY"
     gene            complement(7482..8771)
                     /locus_tag="PK134_03030"
     CDS             complement(7482..8771)
                     /locus_tag="PK134_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRB37852.1"
                     /translation="MKYNSINSKLFVDNRKKFTAELKPKSIAIFQSNDEMPRSGDQNF
                     TFRQNADLFYLTGIDQEESILVIFPDSPLPQYKEILFVRKTNERIAIWEGHKYTKEEA
                     QATSGIKHIYWTEEFNAVVPMLMHHAENVYVNINENDRHATEVPYRDMRFAREWKNRF
                     PAHNFERLGQIMAKLRSRKHDIEVQLISEAIKHTESALRRVLKFMKPGVMEFEVEAEI
                     IHEFVRNRCNGHAYSPIIASGASACVLHYNENNKPCKDGDLVLMDFGAEYANYAADLT
                     RTIPVNGKFTPRQKEVYEAVLRVMKAATKLLVPGNFIEKYHAEVGKMMEEELVNLKLI
                     TKDEIKNQNPEWPAYKKYFMHGTSHFLGLDVHDVGNKYQAMQAGMVFTCEPGIYIPEE
                     SIGIRIENNILITDKAPVDLMATMPVEVSDIESLMRK"
BASE COUNT         2804 a         1537 c         1806 g         2776 t
ORIGIN      
        1 attgtctgaa acatccatct gtctgatttc tgcttcaata tttttgccat tgagagagct
       61 tcaaagctat attaattaat ggaaatctat atccattaac aaactttaac agttagaaat
      121 tgtgttgtcc tgagaaaaat tcacatcaat ttttcagact gattttatat tcataaaaaa
      181 cggttaaact ttcttatttt cgcacactat tttattaatc atgttcgaga atttaagtga
      241 taagttagaa aaggcgttta agaacctaaa aggtcaggga aagataaccg aaattaacgt
      301 tgccgaaact ctaaaagagg ttagaagggc attattagat gctgacgtaa attataaagt
      361 agccaaacaa tttaccgata cgattaaaga gaaggcatta ggtcaaaatg tactaacggc
      421 agtatctccc ggtcagttgt tggttaagat tgcacatgat gaactggcta cattgatggg
      481 tggtcagatg acagatattc aacttaaagg aaatccatca gtcattctaa tgtcagggtt
      541 gcaaggttcg ggtaaaacaa cattatccgg aaaacttgct aattttttaa agactaaaaa
      601 aggaaagaca cctttgttgg ttgcatgcga tgtttacaga ccggcagcca tagatcagct
      661 taaagttttg ggtgaacaga taggtgtaga agtttattct gaagtagaaa ataaaaatcc
      721 tgtagaaatt gcgcgtcatg caataaagca agccaagcag aatggtaaca atgtagttgt
      781 tgttgatacc gcaggtcgtt tagctattga cgagcagatg atgaatgaga ttgcagcatt
      841 gaaaaatgca atcgaacctg atgaaatctt gtttgtggtt gacagtatga ccggtcagga
      901 tgcagtgaat acagcaaaag catttaatga ccgattgaat tttgacggag tggttttgac
      961 taaacttgat ggtgacacaa gaggtggtgc tgcattgtct attcgttcag tagtaaataa
     1021 gcccataaag tttgtcggta ccggtgagaa gatggatgcg ttggatgttt tccatcctga
     1081 gcgtatggcc gaccgtattt taggtatggg cgacattgtg acattggttg aaaaagcaca
     1141 ggaacaattt gatgagaagc aagcagccga gctgcaaaag aagtttgcca agaatcaatt
     1201 taattttaat gatttccttt cgcaattgca gcagattaaa aaaatgggaa acattaaaga
     1261 ccttgtttca atgatacccg gagttggtaa ggcagttaaa gacatggaca ttgacaacaa
     1321 tgcttttaaa agcattgaag caatcattca gtcaatgaca ccggcagaac gtgccaatcc
     1381 tgatctgatt aatggtaaca gaaagaaaag aattgcagcc ggtagcggca caggtattcc
     1441 tgaagtaaat aaattgatta aacagtttga ggatatgcgc aagatgatga agatgatgac
     1501 gacaggtaat atgaaaaaca tgatgcgcaa tatgcccgga atgagaagat aagttatcgc
     1561 attaggacaa tatttttagg gcatgacaat aattgatgga aagcaaacag cagaagaata
     1621 taaatctatt ctaaagcagg aagtaaaagc acttgttgca accggcaagc gtcctccggg
     1681 gttagctata ttactcattg gtaataatgg agcaagcgaa acttatgttg ctaataaatt
     1741 aaagacttgt catgatgttg gatttaatgc atcgcttttt cgttttgaaa atacggtgag
     1801 tgaaaacgaa atattgaact gtataaatga actcaatttt aattcaagca ttgatggtgt
     1861 cattgtgcaa tcaccgtttc caaatcatat ttcaccacag aaaataaatg aagctattca
     1921 tcctgcaaaa gatgttgatg gatttcatcc gatgaatgca gggaggctgc aacagaacaa
     1981 accatgttac attgcagcaa cacctaaagg cattatgcag ttgattgaaa aatataaaat
     2041 tgaaacacag gggaagcatt gcgtggtagt tgggcgaagt aatattgttg gaatgccaat
     2101 gagtattttg ttgagtcgaa acagtaagcc cggaaattgt acagtaacaa tttgtcatag
     2161 caagactgta gatcttgcag catatacaaa acgtgctgat attttaattg cagcaattgg
     2221 ttcgcctgaa ttgataacag cagatatggt gaaagaaggt gcagtagtga ttgatgtagg
     2281 tattacacgt gtagcagaca aagcaacaaa aagcgggtac agacttaaag gagatattaa
     2341 gtatgatgaa gtagccccta agtgtagttt tatcacaccg gttccggggg gtgttggtcc
     2401 gatgaccatt atcggattat tgcagaatac cttacttgcg gcaaaaaatg ccatctataa
     2461 ttaatctatt ttgaaatgat ttttgaacag gaattagcga gcataacaaa tgaagcatta
     2521 aaagtgcttt ttaattttga gccggatgaa agtctgttga cttacacgca aactcgtaag
     2581 gagtttgatg gagattatac tatagttgtt tttccttttg tccgtcagtc gaaaaaatcg
     2641 cctgaagaaa caggaagatt aattggagag tatttaatca aacatgcatc ttttattagc
     2701 aattacaatg tcataaaagg atttttgaat attacggtaa gtgatgagca gtggcttaaa
     2761 agaattgcag ttgcatttgc agattccgaa tatggtttta ctaaagcaga taaatctaag
     2821 cctgttattg ttgagtatgc atcgccaaac acaaacaagc ccatacacct tggacatttg
     2881 cgaaatatcc ttttaggtta ttcagtttca ttgctactga aagcaaccgg acgaaatgtt
     2941 aaacgagtta gcatcatcaa cgacagaggt gtgcatatat gcaaatcaat gcttgcatgg
     3001 aaaaaatttg gtaatgatga aacacctcaa tcatcaggaa tgaaaggtga tcatcttgta
     3061 ggaaaatatt atgtggagtt tgacaagcaa tataaacagc aggtttcaga aatgatttct
     3121 gcaggtattg aaaaagataa agcagaaaag aacgcaccct taatgcagga agtaaggcag
     3181 atgctgatag agtgggagca aggcaatgaa gaagtgactg cattatggaa gaagatgaat
     3241 ggttgggtgt atgaaggttt tgatgttacc tataacagat taggtgtaga ttttgacaag
     3301 acatattatg aatcggaaac ctatctgctg ggaaagaaaa tagttgaaga aggactgtca
     3361 aaaggtgtgt tttataaaaa ggctgacaac agcgtttggg ttgatttaac gaatgatgga
     3421 ttagacgaaa aaatagtgca gcgctctgat ggaacttcgg tttatattac gcaggattta
     3481 ggcactgcag tgttgcgtca tgatgagttt gatttcgatt ctaaaattta tgttgtaggc
     3541 aatgaacagg actaccattt taaagtattg tttttaatac ttaaaaaatt agggtatgaa
     3601 tgggctgaaa agctttatca tttaagttat gcaatggttg atttgccaag tggtaaaatg
     3661 aaatcacgtg agggaactgt tgttgatgca gacgatttga tggacgaagt aactgctgct
     3721 gccgctgaag aaacaagtaa gttgggcaag gttgaagagt ttagcagtga tgaattaaag
     3781 cagctctatg aaacgcttgg ctttggggca cttaaatatt ttattttaaa agttgatcct
     3841 gaaaaaagaa tgttatttaa tcctgctgaa agtgtagaga tacatggaaa cacgggtcct
     3901 tatattcagt atgtgcatgc aagaatttgt tctgttttga agcgggcttc acagatggaa
     3961 caactgagca actataaaaa taccgcaata gaatcacttg ttagcatgaa tgagaaggag
     4021 cgtgaacttg caatcaatat tcttcgtttt aaagaaacag ttgaaactgc agcagaaaaa
     4081 ttttctccgg ctattgttgc aaactatgtt tatgagttag caaaaaactt taatcaattc
     4141 tatcacgata attcaattat ttcagagggt gaattggaaa caagccagtt cagactgtgt
     4201 ttggctgatg cctgtgcaat gacaattaaa aaatcattga acctgcttgg tattaaggcc
     4261 ccggaaagaa tgtaagagat tgtttgagtt gaattaaatt gcctttttca acttagcaac
     4321 agcatccaga acaaactgta cttgttcatc acgactcata taggtgttgt caagaatgat
     4381 tgcatcatca ggctttcgca atgggctttc ttctctgtga gtgtcttcat agtcgcgcaa
     4441 ctcaatattt tttcttattt catcaggtgt tacttttatt cctttttcaa tgagctcatg
     4501 atatcgtctg taaacacgta cttcgggtga tgcagtcata aaaattttca agtcggcatt
     4561 cggaaaaacg gtagtgccaa tatcacgtcc gtccattacc agatttccaa gagttgacat
     4621 ttgttgttgc atggcaacta atttttttcg taccgatttt attgcactca cataactaac
     4681 attgtctgaa acatccatct gtctgatttc tgcttcaata tttttgccat tgagatagat
     4741 ttctgattgt ccggtttgtt catttctgat aaattcaatt tgaatgtttt caagaattga
     4801 agaataatca gcaattacat cagcagtctt gctgaatgga atattgtttt gcaggaaata
     4861 ataagtgact gcgcggtaca ttgcacccga atcaatataa ttcaatttaa gccgctttgc
     4921 tatggctttt gcaaccgtac ttttcccaca ggaagaataa ccatcaatag caattatcat
     4981 aaaacttatc cggaaaactt ttcaaaaata atgaaaagca atataacgac agtgaatatt
     5041 aactttattt caggttagct gtcagttgtt tttggtttgc gcttaaaatc gtttagatat
     5101 gttgaaatag aaaggctgtt ggttccacca gcaagatggt aaaggcttct gccatagctg
     5161 aggtgaaatt tagatatttt taaaccaagt ccgaatgaaa taccaaccat ggcaggtctg
     5221 ctttctactt tcatttcatt tcgtctctgg aaattgtacg ccatgcgcaa atgaaaattt
     5281 ttactgataa gaatctcatt gctgatgatt aaatgtcgca atagtttatt gccaaacgat
     5341 gctttttccg gctcttttac ttcacctgtc agagggtctg tttcctgact gttgttgtca
     5401 atgtaggaaa gatcccattt ttcaatatga cgataggtta aagtaaaacg aaaaggagct
     5461 ttggctaatt tctgtgtaat gcctgcttca atttcaatcg gcagtggctc attgtttttc
     5521 tcagtatatg attttaattg acgaccggca tttctgataa caagtgcagc acttgtgagt
     5581 ttatcgggac tgtaatagtt gactccaagg tcggcagcaa ttccaaatgc agagtaggtg
     5641 tcaatgtgac tgtaaataaa tttcaatgaa gcaccaacaa acaacacact atcaattcca
     5701 acaggtcttg acatagatat gtttaatgca taatcgccac ctgaaaaagt tcctaagtca
     5761 gttgcagttt cgtctgtgcg gtcgaacttc ccatagctta catattgtat gccggcagaa
     5821 agtgttccta tattttcaat gtgatgtgca taggcagtgt aaccatattt tattccggag
     5881 aaatatggat tgtagttgaa ggtaatctgt ttatccatat caacatttaa tatggcagga
     5941 ttgtaaaagg aagtatttaa gtcgttgtcg cgaatggaaa tgaaatttcc accgagtgct
     6001 gcatgacgtg cagatgatgg caggtcaaga aaacgaaaga ttgcacttcc gcctgtctga
     6061 gctttcaaac aaacaggaag caacagtgct gctatcagag ttgttattct catggaattt
     6121 ggtttatgca ctctcaaacg ctaatcgcca aaaaatattt ttatcaaaca aaataaatta
     6181 ataaagagga aaatgttcca tcatgtcgtt gacagtagtc tttacttctt caattacagc
     6241 gccattcgta tgattcataa ttaccatatc aatcaattct acaattcgag ccatttctgt
     6301 ttctttcatt cctcgtgatg tcattgccgg tgttcctaca cgcatacctg aagttacaaa
     6361 aggagattgt gtgtcgaatg gcaccatgtt tttgttaacg gtgatatctg cacgaacaag
     6421 agcattttct gcttgttttc ctgtaatgtt tttagaacgc agatcaatca acattaaatg
     6481 attgtctgtt ccaccggaaa ttaccttgta tccctttttc acaaaggcat tagccatagt
     6541 tttagcattt cgaattacct gtaggcaata ttcaaaatac tcatcgctta atgcttcacc
     6601 aaatgctaca gctttagcag cgataacgtg ttccaatggt ccaccttgcg tgcccggaaa
     6661 aactgcactg tccattaagg cagtcatcat gcgtgtttcg ccttttggag ttttcaatcc
     6721 ccatgggttt tcaaaatctt ttcccatcat gattaaacca ccacgtgggc cacgtaaagt
     6781 tttatgtgtt gtagtagaaa taaagtggca gtgtggcaat ggatcattaa gtaatccccg
     6841 tgcaattagt cctgatggat gtgaaatatc tgcaagcaac aatgcattca ctttatctgc
     6901 aatggaacgt agtgcaacat aatcccaatc gcgagagtag gcagatgcac cgcagataat
     6961 taattttggt ttctctttta aagcagtttc ttcaactttg ttgtaatcaa tagtgcctgt
     7021 attttcttca acaccataaa atgttggacg ataaagtttg cccgaaaaat ttacaggtga
     7081 accatgcgtt aaatgaccgc catgagaaag gttaaagcca agaattgtat caccgggttg
     7141 caggcatgca agcattacgg ctgcattagc ttgtgctccg gaatgaggct gcacatttgc
     7201 ccatgttgca ttaaataaag tcttagctct gtcaattgca atttgttcaa tttcatcaac
     7261 aacttcacag ccgccataat agcgtttgcc gggaagtcct tctgcatatt tgtttgtcag
     7321 acatgagccc atggcgtcca tcacttgttg gctgacaaaa ttttccgatg ctatcaattc
     7381 caaaccatga cgttggcgtg caagttcttc tttaatcagt ttaaaaacag ctttgtcttt
     7441 ttgcatattt gtagagtatg ttttctaatt attttttgtg attatttacg catcaatgat
     7501 tcaatgtcac ttacttcaac aggcattgtg gccatcaggt caactggtgc tttatcggtg
     7561 atgagaatgt tattttcaat acgtattcca atgctttctt cagggatata aatgcccggc
     7621 tcacaagtaa acaccattcc tgcctgcata gcctgatatt tattgcctac atcatgcaca
     7681 tcaagtccga ggaagtgcga tgtgccatgc ataaaatatt ttttataagc aggccactcc
     7741 ggattttgat ttttaatttc gtccttagtt atcagtttaa ggttcaccaa ttcttcttcc
     7801 atcattttac ctacttcggc atgatacttt tctataaagt tgcctgggac aagaagtttg
     7861 gttgcagctt tcatcactct caaaacagcc tcatatactt ctttttgtcg tggcgtaaat
     7921 tttccgttca ccggaatagt tcttgttaaa tctgcagcat agtttgcata ctcagcccca
     7981 aaatccatca gcactaaatc gccatcttta caaggcttgt tgttttcatt ataatgcaaa
     8041 acacatgcag aagcaccgga ggcaattata ggactgtagg catgtccatt gcaacgattg
     8101 cgaacaaact catgaataat ttctgcttca acttcaaact ccatcacacc gggcttcata
     8161 aattttaaaa cacgtctgag tgccgattcc gtatgtttaa tagcttcgct tattaactga
     8221 acttcgatat catgctttcg cgaacgcagt tttgccatta tctgaccaag ccgttcaaaa
     8281 ttatgtgcag gaaaacggtt tttccattca cgtgcaaaac gcatatcgcg ataaggcact
     8341 tctgtagcat gacgatcgtt ttcattaatg ttgacataaa cattctctgc atggtgcatc
     8401 agcataggaa cgacagcgtt aaactcttct gtccaataga tatgctttat acctgatgtt
     8461 gcctgtgctt cttccttagt gtatttatgt ccttcccaga tggcaatgcg ctcattggtt
     8521 tttctgacaa ataatatctc tttgtattgc ggcaatggtg agtcaggaaa tattaccaaa
     8581 atactttctt cctgatcaat accggtgagg taaaacaaat ctgcattctg acgaaaagtg
     8641 aaattctgat cgccactacg cggcatttca tcattagatt gaaatatagc aattgattta
     8701 ggttttaatt ctgctgtaaa ttttttcctg ttgtcaacaa ataacttgct gttgatcgaa
     8761 ttgtacttaa tattcataac taactttact tttaactgtt acaaataacg tgaaataatt
     8821 tttacgtgct ttgttttttt taatgtttta aatgagcctg tgagcaaata tcgggactaa
     8881 aagaatattg acgagcaata tgttaagaat agaagttttt aat
//
LOCUS       DAORQT010000048         8860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1089_length_8860_cov_7.307893, whole genome shotgun sequence.
ACCESSION   DAORQT010000048 DAORQT010000000
VERSION     DAORQT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_8860_cov_7.307893"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK134_03035"
     CDS             <1..551
                     /locus_tag="PK134_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37853.1"
                     /translation="FLFNNGMAYIRNTGQNNLFWVKNEFGFDKDKPWLTRPLDKPFIY
                     KIDSVFWQNPDSILVNTIKSTFKFSPFNKEWWGTYSIKNNTIKIEYFSKTNQESIIHN
                     RYLIELSGYLKNDSTIIITNRICGKWYHCYQCKSDLKNNLNSYTPPVQYNFYSTDFKP
                     DSTQAWFLKKHWYKKGLHQSRK"
     gene            981..1517
                     /locus_tag="PK134_03040"
     CDS             981..1517
                     /locus_tag="PK134_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37854.1"
                     /translation="MKIPIKLKGYYSQLEHIETNVGYPPNVKKVVDTIARNVVFYEDG
                     VFLYSFYGEQFKGNNPIGYKGGVALWGGYSIFNDTLKGFFMTGPDSRPNRPWGYVWFK
                     IIDKNTIRKICFKWQEPITAADVFEFKNTRAKNISNATFIPSDSLPNPNLSWIKNKKW
                     FWCDEQQFKLWKKAQKEK"
     gene            1608..1739
                     /locus_tag="PK134_03045"
     CDS             1608..1739
                     /locus_tag="PK134_03045"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37855.1"
                     /translation="MEELAKDGAILEHARNAVINILNDDPLLNSEENKIFYRHLHQL"
     gene            complement(1766..1963)
                     /locus_tag="PK134_03050"
     CDS             complement(1766..1963)
                     /locus_tag="PK134_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37856.1"
                     /translation="MHALDILLLGGAVVCFVLAYIIASRPARELRQSNKSKSKWNHVN
                     MDSASSVHSPEMMKYVHDKTG"
     gene            2455..3399
                     /locus_tag="PK134_03055"
     CDS             2455..3399
                     /locus_tag="PK134_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB37857.1"
                     /translation="MMSKEILLIDTFHETFIPKLTAMGFHCTEGYEWSLEKIQQSIGN
                     YEGIAIRSRIPLNKDLLQHATNLKFIARGGAGMENIDVAYAVSKNIKCLNAPEANRDA
                     VGEHALMMLLALLNKLMKSDKEVRNGIWLREENRGTELQGKTVGIIGFGNMGSAFAKK
                     LAGFEVNIVACDPYKKIDTAEYPFIKQVSVEQLQQESDIISLHVPLTAETKYMINDTF
                     ISQCKKEIYIINTARGKCLNTADLVKHLKTGKVKGACLDVHEYETSSFEKLETLPEPF
                     GYMIKSDKTILTPHIAGWTHESNRKIAEVLVKKIEQLS"
     gene            3515..4921
                     /gene="glmM"
                     /locus_tag="PK134_03060"
     CDS             3515..4921
                     /gene="glmM"
                     /locus_tag="PK134_03060"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRB37858.1"
                     /translation="MTLIKSISGIRGTIGGKSGESLTPVDVVKFSAAYATWLAAQFPA
                     KTKCKVVLGRDARISGPMIHNLVCGTLAAMGIDVIDVGLSTTPTVEMAVTFHQAHGGI
                     ILTASHNPKQWNALKLLNSKGEFISAADGETLLKIAEQESFEFANVNQLGTVTTDAHA
                     IDKHIAAILALPLVDVKAIASKKFKIVIDCVNSSGGIALPPLLKALGVEEVIELYCTP
                     DGQFPHNPEPLPEHLTELSKTVKKSKAHLGISVDPDVDRLALVCEDGEMFGEEYTLVA
                     VADYVLKNKKGNTVSNLSSTRALRDVTEKAGGSHYASAVGEVNVVEMMKAKNAVIGGE
                     GNGGVIYPDIHYGRDALAGIALFLTHLAKYGNTVSMLRSSYPDYFISKNKIELTPEIN
                     VDGVLESIQEKYKKHKINTIDGVKIEFDKEWVHLRKSNTEPIIRIYAESDSITTANNL
                     AEKIISDIKELISHPAKV"
     gene            4953..6092
                     /locus_tag="PK134_03065"
     CDS             4953..6092
                     /locus_tag="PK134_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRB37859.1"
                     /translation="MKVYFDNAATTPLRQEVIEAMLPVMQKIYGNPSSIHSFGREMRS
                     AIEKARKTIATLLKVAPSEIFFTSGGTEANNMAIRCTIHDHKITHAISSPIEHHAVTH
                     TLEDLEKEGIKLSWVKLNDKGEADYEHLEELLKNNPRSFVSLMHANNELGTVNDIDRI
                     GELCKQYNAIFHCDTVQTMGHFPYDMSQKNIDFFTCAAHKFHGPKGVGFIYINHKLKI
                     HPLITGGAQERNMRGGTENAYGIIGLAKALELAYAELEEDRKHIQGLKEYMKQRLQEE
                     IPGVAFNGDVSARSLFTVLNVSFPPYEGKDMLLFNLDISGIAASGGSACSSGSEIGSH
                     VLRGINADPERPAVRFSFSRYNTKAEVDFCMDKLKALFAVTAVVK"
     gene            6103..6603
                     /locus_tag="PK134_03070"
     CDS             6103..6603
                     /locus_tag="PK134_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB37860.1"
                     /translation="MNLVCLPEEVERLASLREQETLDYFKSIKKKYKSAVIDKAVHLL
                     YAEIAPKIDCTACANCCKKQSPVFTEEEIRPILNHSGISEHENLNELLEHDEINHHYI
                     LRKNPCFLLNKNLCSVYTLRPSSCSEYPHLNRPFFIYRNKSNMSNYKICPIVYNVVEA
                     LKNSLS"
     gene            complement(6620..6787)
                     /locus_tag="PK134_03075"
     CDS             complement(6620..6787)
                     /locus_tag="PK134_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37861.1"
                     /translation="MNLFKPQNDSQVSLLEDIKTLRSLKGNTSRFAILRSVAKTKKHT
                     RTVYPEGWKFE"
     gene            complement(6932..8131)
                     /locus_tag="PK134_03080"
     CDS             complement(6932..8131)
                     /locus_tag="PK134_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37862.1"
                     /translation="MFGIGKRIHLVPLGVFLTIFMFIHGVSTAQTKKTLTINAKFKID
                     GGGSLGDAYMVLQRDGQSIETLPGQSSYTLNLEIGADYILSFNKPGYITKKININTTG
                     ADEERVEQGFESYPFTVVLMKQYEGVNIVVFNQPVAKITYSKKYDEFDYDTDYTKSIQ
                     SAIQKAEDDLKKAEKEFKKNPPKPAKDSTIVANKPKAEEVKPQKEKTKTAPQEEPVAV
                     KEKGKNDDTKNESKNSNKSAAATGGQDQPLKPTQGGVSDEPEGNIDIEKGADQKADIE
                     PTAGNDAIKNKIGLTKGADQPPAAEPAKPQPVVIKTPETAAPEKPKEVVVHTAPEPPE
                     NVSVRREQLAEDRKTVYNVWVTKGNKTVLYSKVVYAWGGIYFFRDLNTSISENMYKQA
                     DAGIVNF"
     gene            8218..8706
                     /locus_tag="PK134_03085"
     CDS             8218..8706
                     /locus_tag="PK134_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37863.1"
                     /translation="MLVVYDRINKEFSKRRKFFLLLLMLSVFSPALRAQENITVQQTL
                     DYINQKFAGKYVVSINYGVLIATYFDGETKYREDQAAIKDLDATKVYYNSKAGMLLIN
                     CTNPQKKCVTREFLGKRLFYSRISFPVSYGQKTNDGLVKAFQHLIHLINDKNYENSEP
                     FE"
     gene            8681..>8860
                     /locus_tag="PK134_03090"
     CDS             8681..>8860
                     /locus_tag="PK134_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37864.1"
                     /translation="MKIQNHLNNVKILFIVLLSAFCVQANAQVDSADFKMRIDSLMNQ
                     GKIKFNNGKFQEALLD"
BASE COUNT         2953 a         1591 c         1626 g         2690 t
ORIGIN      
        1 tattcctttt taataatggt atggcatata ttagaaatac tggtcagaat aatctctttt
       61 gggttaaaaa tgagtttgga tttgataaag acaagccatg gttgacaaga cctttagata
      121 aaccatttat ttacaagata gactctgtat tttggcaaaa tcctgattca attcttgtga
      181 atacaataaa aagcactttc aaattttctc ctttcaataa agagtggtgg ggaacttaca
      241 gtataaaaaa taatacaatt aaaatagaat atttttcaaa aacgaatcag gaaagtataa
      301 ttcataatcg gtatttaatt gaattaagcg gctatttaaa aaacgattcg acaattataa
      361 taaccaacag aatttgtggt aagtggtatc attgttatca atgtaaatca gatcttaaaa
      421 ataatttgaa tagttataca ccacctgtgc aatacaattt ttactcaacc gatttcaaac
      481 ccgatagcac acaggcttgg ttcttaaaaa aacattggta taaaaaaggc ttgcatcaga
      541 gcagaaaata aaaaaccccg tacccgcgaa gttgctcttc ttgggcagtg tgacataaag
      601 ctgtgcttta caaagtgaac aataaattat atttacacaa taaaaaataa aggaaacagt
      661 atgctttcag aagtaaaact tcgttacact aaaacctacg aagtacggga gtatagtagt
      721 tctttggtac aagatagaac tcaaaattgg gtgcataaaa atggtttttt ttatttaccc
      781 tttgggaagg ctccgtatta taaagattat agccaattta aatctggcat gtatttttat
      841 ggcggatata ataatccata ctctttatgg ggaaaataag aaattttatt actatcttaa
      901 cattattagg gttgctattg ggttgtcaac ccacaagccg cgtatttaaa gatgcctttt
      961 cttgttataa agacactagc atgaaaattc cgattaaact aaagggctac tattcacagc
     1021 ttgagcatat tgaaactaat gtgggctatc cacctaatgt aaaaaaagta gtggatacta
     1081 ttgccagaaa tgtggtattt tatgaagatg gagtattttt atattctttc tatggtgaac
     1141 aatttaaagg aaacaatcca ataggctaca agggtggtgt tgcactatgg gggggttatt
     1201 ccattttcaa tgacacacta aaaggttttt ttatgacagg accagacagc aggccaaaca
     1261 gaccttgggg ttatgtatgg tttaagataa ttgataaaaa tacaattaga aaaatatgct
     1321 tcaaatggca agaacctata actgctgccg atgtatttga atttaaaaat acaagagcaa
     1381 aaaatatatc gaatgcaact tttattccgt ctgattcttt accgaaccca aacctttcgt
     1441 ggattaagaa taaaaaatgg ttctggtgcg atgaacaaca atttaaactt tggaaaaaag
     1501 cacaaaagga aaagtaagta ctatttattt taggttctca attaaaattt aactcccgta
     1561 cctgcgaagt tgtacttcgt gggcagcgtg gactactgaa cctgaaaatt gaagagttgg
     1621 caaaagatgg agccatttta gaacatgcaa gaaatgcagt catcaatatt ttgaatgatg
     1681 acccactgtt gaactcagaa gaaaataaaa tattctatcg tcatttacat cagttgtaag
     1741 agcaaaaaaa ccggagtaag gtcagttaac ctgttttgtc atgaacatat ttcatcattt
     1801 cgggcgaatg aactgatgaa gctgaatcca tattgacatg attccatttt gacttagatt
     1861 tattgctttg gcgcaactcg cgtgccgggc gtgaagcaat aatatatgcc agaacaaaac
     1921 agactacagc tcctcctaaa agtaatatgt ctaatgcatg cataacggtt tatttctttg
     1981 ttggtgttgg ttatacttat acacctgcat aagagttacc caaaacctga agattattcc
     2041 gtaaacctta catcattcaa acaaaaaaac atttctcttt gaataactaa gataccaatc
     2101 agcaaacccg gttctctgtt tttaatggtt aaccgataat ggacaatctt ctttttgcgg
     2161 caatttaata aaccatttta gcattgtcaa ctattttctt acttccttag tgaatatttg
     2221 ctgttatttc agccatttgg atacaaaatt tatagaaatt acctttgtgc tattaacttt
     2281 tattctgtat gagaagggag attacgaatc aagaaatgcc taattagcaa ttggcaacaa
     2341 tgacaaatac catcaaattt gtaatcttga gaataaagac acaaacataa agaattaatc
     2401 agacaacatt ctcctgcaca ttcggaggat ttttatttca tgtcaatttt aaaaataatg
     2461 tcaaaagaaa ttttattaat agacaccttt catgaaacat ttattcccaa gctgacagct
     2521 atgggatttc attgtacaga aggatatgaa tggagtcttg agaagataca acaatccata
     2581 ggcaactatg agggtatagc aatcagaagt cgcatacctt taaacaaaga cctgctgcaa
     2641 catgccacta atttaaagtt tattgcccgc ggtggtgcag gcatggaaaa tattgatgtt
     2701 gcttatgctg tttcaaagaa cattaaatgt ctgaatgcac ccgaagctaa tcgtgatgct
     2761 gtgggcgaac atgcattaat gatgttactt gctcttctga ataaactaat gaagtctgat
     2821 aaagaagtac gtaatggcat ctggctgaga gaagaaaacc gtggaacaga attacagggc
     2881 aaaactgtag gcatcattgg ttttggtaat atgggtagtg cctttgcaaa aaaattagca
     2941 ggctttgagg tgaacattgt tgcttgcgat ccatacaaaa aaattgacac tgctgaatat
     3001 ccttttataa aacaagtttc tgttgaacaa ctgcaacagg aatcagacat tataagtctg
     3061 cacgtaccat tgacagcaga aactaaatac atgattaacg atacctttat tagtcagtgt
     3121 aaaaaagaaa tttacatcat caacacagca cgaggtaaat gcctgaacac tgccgacctt
     3181 gttaaacatc tcaaaacagg taaggtaaaa ggtgcatgtc ttgatgtgca tgaatacgaa
     3241 acttcatcat ttgaaaagtt agaaacactg cctgaacctt ttggctatat gataaaatcc
     3301 gataaaacta ttctcacacc acatattgca ggatggacac atgaatcgaa cagaaaaatt
     3361 gcagaagttt tggttaagaa aatagagcag ttatcatgaa aaaagtttct gttgtcagaa
     3421 agaataaaat actgatattc aggaaaacca actattaaat tcataaaatt accctttgat
     3481 ttgatatatt tgcactgtaa aaaaagcaaa gattgtgaca ttaattaaat ctatttcagg
     3541 catcagggga actattggcg gtaaatccgg agagtcactc acccctgttg atgtagtaaa
     3601 attttctgct gcttacgcca catggcttgc agcacagttc cctgcaaaaa caaaatgcaa
     3661 agttgtttta ggtcgtgatg cacgcatcag cggaccaatg atacataact tagtttgtgg
     3721 cacactcgct gccatgggaa ttgatgtgat agatgtgggc ctttcaacca caccaacagt
     3781 tgaaatggct gttacatttc atcaggcaca tggcggaatc attctaactg caagtcataa
     3841 tccaaaacaa tggaatgcac tgaagttgtt aaacagcaaa ggcgaattta tttctgctgc
     3901 cgatggcgaa acacttctta aaattgcaga gcaggaatcg tttgagtttg ccaatgtaaa
     3961 tcagttggga acagtgacaa cagatgcaca tgccattgac aaacacattg cagcaattct
     4021 tgcattgcca ttagttgatg taaaagccat agcatctaaa aaattcaaaa ttgttattga
     4081 ttgcgttaat tcatctggcg gcattgcatt gccaccatta ttaaaagcat taggtgttga
     4141 agaagtgata gaactttact gcacaccgga tggacagttt ccacacaatc ctgagccatt
     4201 acccgaacac cttacagaac tgagcaagac agtaaaaaaa tcaaaagcac atttaggaat
     4261 ttctgttgac cctgatgtag atcgtcttgc attggtttgc gaagatggtg aaatgtttgg
     4321 cgaagaatat accttggtgg cagtagcaga ctacgttctg aaaaacaaaa aaggtaatac
     4381 agtaagtaat ctttcgtcaa cacgtgcgct gcgcgatgtg actgaaaaag ccggtggaag
     4441 ccactatgca tctgccgttg gcgaagttaa cgtggtggaa atgatgaaag ccaaaaatgc
     4501 tgttattggc ggtgaaggaa atggtggagt tatctacccc gacattcatt atggccgtga
     4561 tgcactggca ggcattgcac tttttctgac gcatcttgct aaatacggaa acactgtttc
     4621 tatgcttcgc tcctcttatc ctgattattt tatttctaaa aacaagattg aacttactcc
     4681 tgaaataaat gttgacggag tattagaatc tattcaggag aagtataaaa agcataaaat
     4741 aaatactatt gacggagtaa aaatagaatt tgataaagag tgggttcacc ttcgtaaatc
     4801 aaatactgaa cccatcatcc gcatttatgc agaaagtgac agcatcacta ctgccaacaa
     4861 tcttgctgaa aaaattattt cagatatcaa agagttaata agccaccctg caaaagtctg
     4921 acccccatca cacttaatca aacacacaaa caatgaaagt ttattttgac aatgctgcaa
     4981 caactccact aagacaggaa gttattgaag ccatgttgcc tgttatgcaa aagatttatg
     5041 gcaatccatc atccatccat tcttttggta gagaaatgcg atcggcaatt gagaaagcac
     5101 gtaaaacaat tgctacatta ttaaaagtag caccatctga aatatttttt acttccggtg
     5161 gaacagaggc aaacaatatg gccattcgtt gcaccataca cgatcataaa attacccatg
     5221 ctatttcgtc acccatcgag caccatgcag ttacgcatac gctggaagat ttagaaaaag
     5281 agggtatcaa acttagttgg gtaaagctta acgataaagg tgaagccgac tatgagcatc
     5341 tcgaagaatt actaaaaaac aatccacgtt cttttgtatc gctcatgcat gcaaacaatg
     5401 agttgggcac cgttaacgac attgaccgca ttggcgaact ctgcaaacaa tacaatgcaa
     5461 tttttcattg tgatacagtt caaactatgg gacattttcc ttatgacatg tctcaaaaaa
     5521 atattgactt tttcacttgt gcagcccata agtttcacgg gcctaaaggt gtaggattta
     5581 tttacatcaa ccataaatta aaaatacatc cactgattac aggtggtgca caagaacgca
     5641 atatgcgtgg cggaacagaa aatgcctatg gtattatagg attagcaaaa gctcttgaac
     5701 ttgcttatgc agaattagaa gaagaccgta aacatattca gggattaaaa gaatacatga
     5761 aacaacgttt gcaggaagaa attcctggag tagcttttaa tggtgatgtt agtgcaagaa
     5821 gtttgtttac agtattaaat gtttcttttc cgccatacga aggtaaggac atgttgcttt
     5881 ttaaccttga tatatcagga attgcagcat ctggcggcag tgcctgttcg tcaggcagtg
     5941 agattggctc acatgtatta cgtggaatca atgccgaccc tgaaagacct gctgtacgat
     6001 tttcattttc gcgctacaac acaaaggctg aagttgattt ctgtatggat aaactgaaag
     6061 cattgtttgc agttactgct gttgtgaaat aaattttctc ttatgaatct tgtctgccta
     6121 cccgaagaag ttgaacggtt ggcatcgctg cgtgaacaag aaacgcttga ttatttcaag
     6181 tcaataaaaa agaaatacaa atcagcagtt attgataagg ctgtacacct attgtatgct
     6241 gaaattgcac caaagataga ttgtacggcc tgtgctaact gctgcaaaaa acaaagtcct
     6301 gtttttacag aggaagaaat aagacctatt ctgaatcatt caggcattag tgaacacgaa
     6361 aatcttaacg aattgcttga acatgatgaa ataaatcatc attacattct cagaaaaaat
     6421 ccttgttttt tactcaataa aaatttgtgc tccgtttaca ccctgaggcc ttcatcatgt
     6481 tcagaatacc cacacctgaa ccgtcctttt tttatctatc ggaataaaag taatatgagc
     6541 aattataaaa tttgcccaat agtgtataat gttgttgagg cattaaaaaa ttcgcttagc
     6601 taaaagaaag attaaaaact tattcaaact tccagccttc gggataaaca gtgcgtgtat
     6661 gcttttttgt tttggctaca gagcgaagaa tagcaaacct tgatgtattc ccttttagag
     6721 aacgcagtgt tttaatatct tcaagtaacg aaacctgact gtcattttgc ggtttgaaca
     6781 gattcatgca gaaatatgta ttcaacattt tattgcagtg tttattttct ccatgcaatt
     6841 taaaagccgc tgaatacaga aatatgttaa gttgggtgaa tacctgaaaa cgaaaggtaa
     6901 accactgcaa tttttcagtt gaagaagaca cctagaaatt tactattcct gcatcggcct
     6961 gcttatacat gttttcagaa atagaagtat tcaggtcgcg aaaaaaataa atgccacccc
     7021 aggcataaac gaccttacta taaagcaccg ttttattgcc cttagtaacc caaacattgt
     7081 aaacagtttt tctgtcttcg gccaattgtt cacgtcttac tgaaacattt tcaggaggct
     7141 caggtgctgt atgaactact acttcttttg gtttttcagg tgcagcagtt tctggagttt
     7201 taataactac cggttgtggt ttagctggtt ctgctgcagg tggttggtca gcaccttttg
     7261 tcaagccaat tttattttta attgcatcat tccctgctgt gggttcaatg tctgcctttt
     7321 gatctgcgcc cttttcaatg tctatattac cttccggctc atcacttact ccgccttgtg
     7381 taggctttag tggttggtct tgtccacccg tagcggcagc gcttttatta gaatttttcg
     7441 actcattctt cgtatcatca ttcttgcctt tttcttttac agcaaccggt tcttcctgtg
     7501 gtgcagtctt ggttttttct ttctgaggtt ttacttcttc tgccttaggt ttgttggcaa
     7561 caattgtgct gtctttggca ggtttgggcg gattcttttt aaactctttc tctgcttttt
     7621 tcaaatcatc ttctgccttt tgaattgccg attgaattga cttggtataa tcagtatcat
     7681 aatcaaactc atcatacttt ttcgaataag taatttttgc aacaggctga ttaaacacta
     7741 caatatttac gccctcatac tgcttcatca acactactgt aaaaggatag gactcaaaac
     7801 cctgctcaac tctttcttca tcggcaccgg ttgtgttaat atttattttt ttggtgatgt
     7861 atccgggctt gttaaaagac aagatataat ctgcccctat ttcaagattt aaagtatatg
     7921 acgactgccc tggaagagtt tcaatagact gaccatcacg ctgcaaaacc atataggcat
     7981 cacccaaact tccacctccg tcaatcttaa acttggcatt gatggttaat gttttctttg
     8041 tctgcgcagt ggaaacacca tgtataaaca tgaagatagt gagaaaaacc cctaaaggaa
     8101 ctaaatgaat tcttttccca atgccaaaca tttgtaattt tattccgtta aaaagtttca
     8161 atgccgatac aaaattagtt aatagtttaa acgatagtcc taatttccaa aataagaatg
     8221 ttggtggttt acgatagaat caataaagag tttagcaaaa gaagaaagtt ttttctgttg
     8281 ctgctgatgc ttagtgtttt ttcacccgca ctcagagcac aggaaaacat caccgtacaa
     8341 caaacgctcg actatatcaa tcagaaattt gcaggcaaat atgtagtttc tatcaactat
     8401 ggcgttttaa tagccactta ttttgatggc gaaacaaaat acagagaaga tcaggcagct
     8461 attaaagacc ttgatgccac taaggtttac tacaattcaa aagcaggaat gttgctgatt
     8521 aactgcacca acccacaaaa aaaatgtgtc acccgtgagt ttctgggaaa aagacttttc
     8581 tacagccgca ttagttttcc ggtttcttat ggacaaaaaa ccaacgatgg cttggtaaaa
     8641 gcatttcaac atttaataca tttgattaat gataaaaatt atgaaaattc agaaccattt
     8701 gaataatgta aaaatattgt tcattgtatt gttatctgcc ttttgtgtac aggcaaatgc
     8761 acaggttgac tctgctgatt ttaaaatgcg cattgactca ctgatgaatc aggggaaaat
     8821 aaaatttaat aatggtaaat ttcaggaagc attgttagac
//
LOCUS       DAORQT010000049         8833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1095_length_8833_cov_15.184552, whole genome shotgun sequence.
ACCESSION   DAORQT010000049 DAORQT010000000
VERSION     DAORQT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_8833_cov_15.184552"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK134_03095"
     CDS             <1..639
                     /locus_tag="PK134_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HRB37865.1"
                     /translation="NAHNLVVYGKTDDRYLISDPVMEDTTSLTEFDLNRVRFAKGPLA
                     PKGQLYYPQIENIVSDEKIVYAIKRGIKQNIRDMLYIPGPIAGVSGIKYVAKKILKWH
                     DRLGAKTTGLYLAQLVRMQEEIGTGGGGFRYIYAAFLQQSHAYLKNDSIIKIAEQFTK
                     AGDMWRDSAIQSAGIFKGRLTDRADFVNMHDRLMQIAEVEKNAFTDLSKVKL"
     gene            653..1393
                     /locus_tag="PK134_03100"
     CDS             653..1393
                     /locus_tag="PK134_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB37866.1"
                     /translation="MDVLTVSSLSYTYKNSDHAVLSNINLHVGKGEVFGVFGPNGAGK
                     TTLLSLVTGILTCRLGVISILGKDASTQRKNVNQIIGYVPQDFAFYEELSAIENLIFF
                     GAMYGLKEQALDNRAKELLEEFGLMNVAERYVKEFSGGMKRRLNIAIGLLHKPQLLIL
                     DEPTVGVDVQSRLAIMSYLKKINSEGTTIIYTSHLLKEAEEFCSRIALIDKGKVIAID
                     TIANLLIQNQCKSLEEVFIKLTGNNYRD"
     gene            1393..>1840
                     /locus_tag="PK134_03105"
     CDS             1393..>1840
                     /locus_tag="PK134_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37867.1"
                     /translation="MSPLVASIIKEFKVLSRDKTGLLLMFLMPLVLALLMTVLQNSAF
                     KIVNENKVDLMLINNDSNSLFFQNFCKGIEQTGMFDLKINTKEISSKKLSEDKESIVV
                     VTIPKGYAYTLDQEVKAKAEQSLQMFGMLESVSTKGIIGVKNIELSF"
     assembly_gap    1841..1867
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1868..2691
                     /locus_tag="PK134_03110"
     CDS             <1868..2691
                     /locus_tag="PK134_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37868.1"
                     /translation="PVLQESYRFSIKSTVLSVVMAIQNEMLLKQLFYDVNQSEPDSAA
                     LSKILPKPLLLSESLIKENSVANPNATQHNIPAWTVFAMFFTVISLGGNIVREKRSGS
                     FLRIKTLPISITQMLIAKQVVFWIVTVLQTLVLFSIGILIFPMLQLPPLVISSGLLPL
                     FLATLCCGLCAVSFALCIGVYSDTVEQANGFGAASVVILAAVGGIFVPLFAMPEVFKL
                     IANVSPFSWAMKAYYSAILEISPIHKLLLNVLPLLLLTAILQLIAFIGLKRKNLL"
     gene            2688..2957
                     /locus_tag="PK134_03115"
     CDS             2688..2957
                     /locus_tag="PK134_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRB37869.1"
                     /translation="MTKEEIKPELKKHIIKYLNLLDLTPESIKDDQPLFGEGGLGLDS
                     IDSIELIVLLEREYGIKITDPKEGRKILIDVNTIADYIIQHKSAK"
     gene            2969..4162
                     /locus_tag="PK134_03120"
     CDS             2969..4162
                     /locus_tag="PK134_03120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRB37870.1"
                     /translation="MENVCITGVGLVTAVGNNANETILAIQSGKTGIGIPKYVKGRYA
                     SMIPFGEVKLSDMQLADELSIRNKVVSRTQLLAHKVFNEALNNSALHIDDLKNTETSI
                     VVSSTVGGMYLTDELYADSNNLSKGSDYLSLYDCASIGIYLQQLTGAKGVINTINTAC
                     SSGANALIYGMRLLKNKRAKRVIVGGVDCLSKFTINGFNALNILSTRSCRPFDNNRDG
                     LNLGEGAGFLILESSDTAKEEKVLAYIKGYANSNDAFHPSALSDEGFGPMAAMRGALE
                     SAGLMPKEINSVIAHGTGTPNNDRVEGLSIKRIFGESLPPVCSLKSILGHTLGAASAV
                     ESVLAVIGVRDGHVYPSRNFETPDEETGLVPNLNFKKEKIKHSLLNSFGFGGNCSSLI
                     FSSVN"
     gene            4193..5203
                     /gene="obgE"
                     /locus_tag="PK134_03125"
     CDS             4193..5203
                     /gene="obgE"
                     /locus_tag="PK134_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRB37871.1"
                     /translation="MEKNFVDYVKICCRSGAGGPGYVHFHRDKKTAKGGPDGGDGGRG
                     GHIIVRGNKQLWTLLHLKYRKHILADAGQKGGNALRTGADGKDEILEVPIGTIARDFE
                     TNKFLFEIEEDGQEVILARGGRGGKGNAFFKTSTLQTPRHAQPGGEGIEEWKILELKL
                     MADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPNLGMVYYHDDRSFVMADIPG
                     IIEGAHTGKGLGLRFLRHIERNSILLFLIAADAKSIKEEYKILKNELRQHNPELLDKR
                     RVIAITKSDMLDEELLKELKKEVPNDKNPGGGKVPVVFISSVANFNIDKLKDVLWKEL
                     NS"
     gene            5200..5763
                     /locus_tag="PK134_03130"
     CDS             5200..5763
                     /locus_tag="PK134_03130"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRB37872.1"
                     /translation="MIEQIKEFDTNLFLWLNSIHSPAMDSFFFAITNRFVWIPVYLIL
                     LFLLIKKKKNKTWSILLFIVILIICSDQLSVLIKENVMRYRPCHNLNIQGVVHTVNNK
                     CGGKYGFVSSHATNTMALFAFLVLSVFKKERIVIALLAIYVLLVSYSRVYLGVHYPFD
                     ILGGWITGLAVALVLYYFVYNKLFNQP"
     gene            5799..6173
                     /gene="crcB"
                     /locus_tag="PK134_03135"
     CDS             5799..6173
                     /gene="crcB"
                     /locus_tag="PK134_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRB37873.1"
                     /translation="MRTILTIGFGSFIGGMIRYMLSLFIQSKTVSEFPVGTLLVNIIG
                     CFLIGVVYSFAEQGKMAVEWRLFIATGILGGFTTFSAFSMESFMMLRNGYYGMMTVYV
                     FLSLAIGISATIGGFLLVRTLN"
     gene            complement(6227..6862)
                     /locus_tag="PK134_03140"
     CDS             complement(6227..6862)
                     /locus_tag="PK134_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRB37874.1"
                     /translation="MIEPVISGFLLGCFLAILAGPVFFLIINISLRKGFSYASRVAIG
                     VLISDTLFIFIAYFGSNFILLLNNHRFIAGIISGILLIGFGIALILKRNSIPAEAITV
                     NEKTDSKSLYILKGFMINSMNPSVLFFWIGVSGTVSVKEHYTIHHLLLFYGSILITLF
                     SFDLFKAYIAHKLKSILTINLLTWLNRITGMALLGIGLYTLIVACWQAYAK"
     gene            complement(6882..8429)
                     /locus_tag="PK134_03145"
     CDS             complement(6882..8429)
                     /locus_tag="PK134_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRB37875.1"
                     /translation="MTLIGRIIKASIDLRGKIEIVPFSPFISQKRVLKKLIRKAQFTM
                     FGEAYNFQGMLGNKNFVTKFRAAVPVHDYNSIFSNWWNKSLHDQENVCWPGKVRYFAL
                     SSGTSGSSSKHIPVTKDMSKAIQKASLKQILSLSKYDLPFSTFEKGILMLGGSTHLHQ
                     QGSYFEGDLSGITASQIPFWFQHFYKPGRKISREKDWHAKLDEITHKAKDWDIGIIVG
                     VPAWVQLLIEKILDHYKLKNIHELWPNLEIYVHGGVSFEPYKKGFSKLLGKQIYYIET
                     YLASEGFIAYQDEPNTRGMRLLVNNGIFYEFIPFNEDNFDADGELKHKPKTLLLNEVK
                     ANEPYAILLTTCSGAWRYLIGDVITFTNLEKCEIQIIGRTKHYLSLCGEHLSVDNMNR
                     AIELVSDELNIDIPEFGVAGIPYQSLFAHKWYIGCDTYNDSENLKKLIDEKLKILNDD
                     YRVERRSALKEIFVEIIPTSWFYKWMELQGKIGAQNKFPRVMKNKVFEDWEKFVASQH
                     KNINQAV"
     gene            complement(8526..>8833)
                     /locus_tag="PK134_03150"
     CDS             complement(8526..>8833)
                     /locus_tag="PK134_03150"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37876.1"
                     /translation="FNGTMHDLKTIYAPSCQNDEFKLLSVNQVEDIVNSIVSSPDNIK
                     AEITLFDLKGALIFKEEVSLSTGVNSVRLPINLAKGLYYFQIINDNQVTQGRKFLVN"
BASE COUNT         2871 a         1308 c         1732 g         2895 t
ORIGIN      
        1 aatgctcaca atcttgttgt ttatggtaag acagatgaca ggtatttaat cagcgatccg
       61 gtgatggagg acacaacctc tttaacggag tttgatttga acagagtgcg ttttgctaaa
      121 ggacctttgg cacctaaggg acaattatat tatccacaaa ttgaaaatat tgtttctgac
      181 gagaaaatcg tttatgcgat taaacgtggt ataaagcaaa acataagaga tatgctttat
      241 atacccggac caattgccgg tgtaagcggg ataaaatatg ttgcaaaaaa aattttaaaa
      301 tggcatgatc gtttaggagc aaaaaccaca ggtttgtatt tggcgcagtt ggtaaggatg
      361 caagaagaaa taggtacagg tggtggtgga tttcggtata tctatgcagc atttttgcag
      421 cagtctcatg cttatttaaa aaatgacagt atcattaaaa tagcagaaca gtttacaaag
      481 gccggtgata tgtggcgcga ttcagcaata cagtcagcag gaatatttaa aggccggctt
      541 acagacaggg ctgactttgt gaatatgcat gaccggttga tgcagattgc agaagtggaa
      601 aaaaatgcat ttactgattt gtcaaaagta aaactgtgaa agcataaact ttatggatgt
      661 actcactgta tcatcattat cttatactta taaaaatagc gatcatgctg tgttgtctaa
      721 cattaacctg catgtcggta agggagaagt ttttggtgtg ttcggaccaa atggtgcagg
      781 taaaacaaca ttgttatcat tggtcacagg tattttaaca tgtaggttgg gggtgatttc
      841 tattttaggg aaagatgcat ctacccaacg aaaaaatgtt aatcagataa taggatatgt
      901 gccacaagat tttgcattct atgaagagtt gagcgctata gagaatctga ttttttttgg
      961 tgccatgtat ggattgaagg aacaagcttt ggacaacaga gcgaaagaat tgcttgagga
     1021 atttggatta atgaatgttg cagaaaggta tgtcaaagaa ttttcaggcg gaatgaaacg
     1081 tagattaaac attgcaattg gtttgttaca taaaccacaa ttgctgattc ttgatgagcc
     1141 aactgtagga gtggatgttc agagcagatt ggctataatg agttatctga aaaaaataaa
     1201 ttctgagggc acaacaataa tttatacttc acatttgctt aaagaggcag aagaattttg
     1261 ttctcgtatt gcattaattg acaaaggaaa agttatagca attgatacaa tagcaaattt
     1321 gctgatacag aatcaatgta agtcacttga agaagtgttt attaaattaa caggtaataa
     1381 ttacagagac taatgagccc attagtagca tcaattatta aagagtttaa agtcctttcg
     1441 agagataaga ccggactgct tttgatgttt ttaatgcctc ttgtacttgc attacttatg
     1501 actgttttac aaaacagtgc atttaagatt gtcaacgaaa acaaagttga tttaatgtta
     1561 attaataatg attctaatag tctgtttttt cagaattttt gtaaaggcat tgagcaaacc
     1621 ggcatgtttg atttgaaaat taatactaaa gagatatcaa gcaaaaagtt atcagaagat
     1681 aaagagtcaa ttgtagtagt tactataccc aaagggtatg cttatacact tgatcaggaa
     1741 gtaaaagcaa aggcagaaca gtcattgcaa atgtttggta tgcttgagtc agtttcaaca
     1801 aaaggaataa taggagtaaa aaatatagaa ctgagttttg nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnatc cggtacttca ggagtcatat cgtttttcaa ttaagtcaac ggttttatct
     1921 gtagtcatgg ctattcagaa tgaaatgtta ttgaaacaac ttttttatga tgtcaatcaa
     1981 tcagaacctg attctgctgc attgagtaag atattaccaa agccattgtt gctaagcgag
     2041 tcattaatta aagaaaatag tgttgcaaac ccaaatgcaa cacaacataa catacctgca
     2101 tggacagtat ttgccatgtt ttttactgtg atatcgttag gtggaaacat tgtcagagaa
     2161 aaaagaagcg gaagtttttt acgaataaaa actttgccca tatcaattac ccaaatgttg
     2221 attgctaaac aagtggtctt ttggattgtg acagttttac aaacgttggt cttgttttcg
     2281 ataggaattc taatatttcc tatgttgcaa ttgccgccat tggtaatttc atcagggtta
     2341 ttgccattgt ttttggctac attatgttgt gggttgtgtg cagtaagctt tgccttgtgt
     2401 attggtgttt attctgacac ggttgagcag gctaatggct ttggtgctgc ttctgttgtt
     2461 atactggcag cagtaggagg tatttttgtt ccattatttg caatgcctga agtttttaag
     2521 ttaattgcta atgtttcacc attcagttgg gcaatgaaag cttactattc agcaatatta
     2581 gaaatatcac caattcataa gctactgcta aatgttttac ctttgctttt gctgacagca
     2641 atccttcagc ttattgcctt tatcggatta aaacggaaaa atttattatg acaaaagaag
     2701 aaattaagcc agagttaaag aaacatatta ttaaatattt aaacctgctt gacttaacac
     2761 cagaaagtat aaaagatgac caacctctgt ttggagaagg aggattaggg ttagattcaa
     2821 tagattccat tgaacttatt gttcttttag agcgtgagta tggtataaaa attactgacc
     2881 ccaaagaagg aagaaaaatt ttgattgatg tcaatacaat tgctgattat attattcagc
     2941 ataagtctgc aaaataattt tttgtattgt ggagaatgtt tgcataacag gagttggttt
     3001 ggttacagct gtcggaaaca atgcaaatga aaccatcctt gctattcaaa gtggaaaaac
     3061 aggaataggt attccaaaat atgtaaaagg cagatatgct tcgatgatac catttggaga
     3121 agtaaaattg tctgacatgc agttagccga tgaattatca attagaaata aagttgtcag
     3181 tcgcacgcaa ttactggccc ataaagtatt taatgaggca ctaaataata gtgcattaca
     3241 tatagatgat ttgaaaaata cggaaacgtc aatagttgtt tcaagtactg ttggtggcat
     3301 gtaccttact gatgaattat atgcagattc aaataatctt tctaaaggtt ctgattatct
     3361 ttcattgtac gattgtgctt caattggaat ttatttgcag caacttaccg gagcaaaagg
     3421 tgtgataaat actattaaca cagcttgttc gtccggggca aatgctttga tatatggaat
     3481 gaggctatta aaaaataaac gtgctaaaag agttattgtt ggtggtgtag attgtctttc
     3541 taagtttaca ataaatggat ttaatgcact gaatatattg tcaacaagaa gttgcagacc
     3601 atttgataat aatcgtgatg gattaaatct tggtgagggt gcaggttttc ttatactcga
     3661 gtcgtcagat acagcgaaag aagaaaaagt acttgcttat atcaaaggtt atgctaattc
     3721 aaatgatgct tttcatcctt cagctttgag tgatgaaggt tttggcccaa tggcagcaat
     3781 gcgtggtgcg ttggaatctg cgggattgat gcctaaagaa attaacagtg taattgccca
     3841 cggcacaggt acacccaata atgacagagt agaaggatta tctataaaaa gaatttttgg
     3901 agaatcactg ccaccggtat gttctttaaa atctatttta ggacatactt tgggtgctgc
     3961 atcagcagta gaaagtgtgc tggctgtaat tggagtaaga gatggccatg tttatccaag
     4021 cagaaatttc gaaactcctg atgaggaaac cggtcttgta cctaacttga atttcaagaa
     4081 agaaaagata aagcatagtc ttcttaattc atttggattt ggaggtaatt gtagctcatt
     4141 aattttttcc tctgtaaatt gaaaatagaa cagtttaaaa cagttaaatt aaatggagaa
     4201 aaattttgtt gattatgtta aaatttgttg ccgttcagga gcaggaggtc cgggctatgt
     4261 acattttcac agagataaaa agaccgcaaa aggtggacct gacgggggcg atggtggacg
     4321 aggaggacat attattgtgc ggggtaacaa acaactttgg actttattgc atttgaaata
     4381 tcgtaaacat atacttgcag atgcaggaca aaagggaggt aatgcattac gtacaggtgc
     4441 tgatggtaaa gatgagattc ttgaagtacc aataggaacc attgcaagag attttgaaac
     4501 caataaattt ttgtttgaaa tagaagaaga tgggcaggaa gtgatattgg caagaggagg
     4561 gagaggaggc aagggaaatg cttttttcaa aacatctaca ttacaaactc cacgtcatgc
     4621 gcaacccggt ggcgaaggta ttgaagaatg gaaaattttg gagttaaaac ttatggcaga
     4681 tgttgggttg gttggttttc caaatgcagg aaaatctaca ctactttctg ttgtgtctgc
     4741 tgccaaacca gaaattgcag actatccatt tacaacctta gttcctaatc tgggcatggt
     4801 ctattatcat gatgacaggt cgtttgtgat ggctgacatt ccaggaatta ttgaaggtgc
     4861 acataccgga aaagggttgg ggttaagatt tttaagacat attgagcgta attcaatact
     4921 gttatttttg attgctgcag atgcaaaaag tattaaagaa gagtataaaa tacttaagaa
     4981 cgaactacgt cagcataatc ctgaattgtt ggataaaaga agggttattg ccatcacaaa
     5041 atcggatatg ttggatgagg agttgttgaa agaattgaaa aaagaagtac ctaatgacaa
     5101 aaatcccgga ggaggcaaag tgcctgtagt ttttatttcg tctgttgcga attttaatat
     5161 tgataaactt aaagatgttt tgtggaaaga acttaattca tgattgagca gataaaagaa
     5221 tttgatacaa atttattctt atggctgaac tcaatacata gtccggccat ggattctttt
     5281 ttctttgcta ttaccaatcg ctttgtatgg attccggttt atctgatttt actttttttg
     5341 ctaataaaga agaaaaaaaa taaaacatgg agcatactgt tgtttattgt tatattaatt
     5401 atctgcagtg atcagctttc tgttttgata aaagaaaatg taatgcgtta tagaccttgt
     5461 cataatctga atattcaggg tgtagtacat acagttaata ataaatgtgg aggcaaatat
     5521 ggttttgtat catctcatgc aacaaataca atggctttgt ttgcgtttct tgtattgtca
     5581 gtttttaaaa aagaaagaat agttattgct ctattggcaa tctatgtctt attagtttca
     5641 tatagcagag tatatttagg ggtgcattat ccgtttgata ttttaggtgg atggattaca
     5701 ggtttggcag tagcattagt tttgtattat tttgtatata ataaattatt caatcagcct
     5761 taatataggc atatagatta tgcttttagt gatttaatat gaggacaatt ttaacaatag
     5821 gttttggtag ttttataggc ggcatgattc gatacatgct ctcacttttt attcaatcca
     5881 agacagtttc agaatttccg gtcggcacct tgcttgtaaa tattatcggg tgctttttaa
     5941 ttggcgttgt gtatagcttt gccgaacaag gtaaaatggc agtagagtgg cgcttattta
     6001 ttgctacagg aatacttggt gggtttacaa cgttttcagc tttttcaatg gagtcgttta
     6061 tgatgttgcg taatggttat tatggaatga tgactgtata tgtttttcta agtttggcta
     6121 ttggtatatc tgctacaata ggtggttttt tattggtaag aactcttaat tgatagttaa
     6181 ggaataagtg taatttttga tatttgatgc ttataatggt tgtgttttat tttgcatagg
     6241 cctgccaaca ggcaactatc aacgtatata agccaattcc aagtaatgcc atgcctgtaa
     6301 tcctattgag ccaggtgagt aaatttattg tcaatataga ttttaattta tgtgcaatgt
     6361 aggctttaaa tagatcaaaa gagaagagtg tgattaaaat agaaccgtaa aacaacaaaa
     6421 ggtgatgaat ggtgtagtgc tcttttacag aaaccgttcc cgaaacacca atccagaaaa
     6481 atagcacaga aggattcatg gaattaatca taaatccttt taaaatataa agactcttgc
     6541 tatctgtttt ttcgtttact gttatagctt cagcaggaat actatttctt ttaagtatta
     6601 aagctattcc aaaaccgata agcaaaattc ctgaaattat tcctgctata aatctgtgat
     6661 tgttgagtag tagaataaaa ttacttccaa agtaggctat gaaaataaaa agtgtatcac
     6721 taatgagtac acctattgcc actctacttg catagctaaa gcctttgcgt aagctgatgt
     6781 ttatgatgag aaaaaaaacc ggtccggcaa gaattgccag aaagcaacct aacagaaatc
     6841 cacttattac aggctcaatc aaaattgtag tttataaaaa attaaactgc ttgatttatg
     6901 ttcttatgtt gtgatgcaac aaacttttcc caatcctcaa aaactttatt tttcattact
     6961 ctgggaaatt tattttgtgc accaattttg ccttgtagct ccatccattt gtaaaaccaa
     7021 gatgttggaa ttatttcaac aaaaatttct ttcaatgcac ttcttctctc aaccctataa
     7081 tcatcattaa gaattttcag tttttcgtca ataagctttt tcagattctc tgagtcatta
     7141 taagtatcac aaccaatata ccatttgtgt gcgaataaag attgataggg gatacctgca
     7201 acaccaaatt cagggatatc aatattcaat tcatcagaaa caagttcaat tgctctgttc
     7261 atgttgtcaa cagacaggtg ttcaccacat aaactcagat agtgttttgt tctaccgata
     7321 atctgaattt cacatttttc taaattagta aatgtaatta catcaccaat taaataacgc
     7381 catgcaccgg aacaagttgt taataatatc gcataaggtt cattggcctt aacttcgttt
     7441 agtaataaag ttttcggttt gtgttttaat tcaccatcag catcaaaatt atcttcgtta
     7501 aatggtatga actcataaaa tattccattg ttaacgagaa gtctcatacc tcttgtgttt
     7561 ggctcatcct gataggcaat aaatccttct gatgctaaat aggtttctat gtaataaatt
     7621 tgtttcccta agagtttgct aaatcctttt ttataaggct cgaaggagac accaccgtga
     7681 acataaattt caaggttggg ccacagttcg tgaatatttt taagcttgta atggtcaagt
     7741 attttttcaa tcaaaagttg aacccaggca ggaacaccta caataatacc tatgtcccaa
     7801 tcttttgcct tgtgcgttat ttcatcaagt tttgcatgcc aatctttttc tctggatatt
     7861 tttctgccag gcttataaaa atgttgaaac caaaatggaa tctgacttgc tgtgattcca
     7921 ctaagatcgc cttcaaaata actgccttgc tgatgtaagt gtgtactacc gcctaacatt
     7981 aatatacctt tttcaaaagt tgagaaaggc aggtcgtatt tagataaaga aagaatctgt
     8041 tttaagctgg ctttttgaat ggctttcgac atatcctttg ttacgggaat atgtttgctt
     8101 gatgaacccg aagtgccgga acttaaagca aagtaacgaa ccttgccggg ccaacaaaca
     8161 ttttcctgat catgtaaact tttattccac caatttgaaa aaattgaatt gtagtcatga
     8221 actggaactg ctgccctgaa tttagttaca aaatttttat tccccagcat accttgaaag
     8281 ttgtaagctt caccaaacat ggtaaactgg gcttttctta tcaacttttt cagtacacgt
     8341 ttctgagaaa taaaaggaga aaagggtaca atctcaattt ttcctctgag atcaatactt
     8401 gccttaatta ttctgcctat caaagtcatg ctaaaatgtt tgcaaggcta aaaataggaa
     8461 atactttgaa atgcaataaa aaaacccatg aattgacatg ggtttttatt aaattgtact
     8521 tattcttagt tgaccaagaa ttttctgcct tgggttacct gattgtcgtt aataatctgg
     8581 aagtaatata atccctttgc aaggtttata ggtaggcgaa ctgaattcac tcccgttgaa
     8641 agcgaaactt cttctttaaa aattaaagct ccttttaagt cgaataaagt tatttctgct
     8701 tttatattat cgggacttga aacgatagag ttaacaatat cttcaacttg gtttacagac
     8761 aataatttaa actcatcatt ttgacatgat ggtgcataaa ttgtttttaa atcatgcatg
     8821 gttccattaa aat
//
LOCUS       DAORQT010000050         8772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1108_length_8772_cov_9.805897, whole genome shotgun sequence.
ACCESSION   DAORQT010000050 DAORQT010000000
VERSION     DAORQT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_8772_cov_9.805897"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK134_03155"
     CDS             <1..341
                     /locus_tag="PK134_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37877.1"
                     /translation="NEQTDATLRGMLGFANGKYSKRIYQSFDGVKTLQVTENASSVID
                     IALNLGFEVKYSFHKNVGVMFAGDYFRTEFNYDDVYLIYSENGEKRSTGAYSQPVDVF
                     QFSAGIFTKF"
     gene            660..5837
                     /locus_tag="PK134_03160"
     CDS             660..5837
                     /locus_tag="PK134_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37878.1"
                     /translation="MLAVPTCLVAQNYQPKSYYTFEGSSPMKDSMGNFNLDPNYYQSS
                     YQILTNTSGGVGKYMKLDSSSTIIRGGVLAIDSAITFEFIFKPGMNFGTTVFAKRMDG
                     ALELRMTYPAINFMTEVKAANGSPIYDDFEIDLNGIGRASYSYYVDDNWHHIVFKYNA
                     KAGIKEVWVDGQLPAGFSKTIAGGSFSQSGNRDYMLNSQSDYYKLQGAIDEIAVYHNS
                     LGGNSIYAHYLNFMAGKHYSFANPTVVAPSPSPVTAGININDFAPGHPSPSLTPIEQL
                     KNFPTPRYKPGHTLFPNNPFVGYYYLSGYQTYETVYSKAVANSVTLQTDFAKNFNYTI
                     MAAENTGAYSDYSNSTTFSHAWINLANSYPQYKVSALSYWPQLRPTDAGYKSNSPYAT
                     CGCLPDKYYLKNSSGQYLNPNGGTGSTKYLSPAGAPDSLVKDGLTQKFYMQQLLAKLS
                     RPIDYILDNGEVVPFLYYTNVMQSDPSVVSDKNSSGLDWFTYEGRRAATKINAYRDQF
                     MGLSGLSNTKFAYYQLDGQPTWNLKWSEVRTVNTAMNGSHYATGDIYMRWPSNWRYWS
                     GAWKGWQWVVESRREQLLAGDKYFSPAVSPGWDNDETQNVRPAQWLGFLKAVSMTGAE
                     FFTTGYFVTSQPYQNPKNYIWQLVIPPYAQAITSRYEDLFRNGYLMSGDVPNSTTSPT
                     YMAYSFNTGDPGKLVVVRKHNSQNKYAITGSSQPMSNMAGAAPDEAVAQINLDGSRIK
                     FKIRRQGSTYIYDNTNTSAPIFYQLDGWHENTYPYYWSKDFKFEAELNDNSNSNVYLK
                     TTRPAGAAAGDFTDFTTAVGFNSVSDLTYNFQPRSTTDYTYYVWVRARSKNSQTTGMT
                     ISLDGSNSKQIGCITDANWQWYKYDVNSGAVISFTSVTSLADHILKITPTNTNLEIDQ
                     IHLSTSSNAIYTSPAPCSGVAVPTISASGPVAFCAGGNVNLIASNGLSYLWSNGSTDP
                     YINVTSAGSYVVTVTTAAGTATSAPVTVTVYAQPNATITANGSTSFCVGGSVTLTASS
                     GSTYLWSSGATTQSITAIGGGNYNVIVTNSNGCSAMSASTAVTVKPVPTATISPSGPT
                     TFAAGGSVTLTSSSAGTGGSYKWSPGGMTTQAVTVSTTGNYVVTVTNNSGCSATSSPL
                     SVTVTSTAPTPKITANGPTTFCQGGSVVLTCSAANSYLWSNGATTQSITVTTAGSYTC
                     TATTAGGSATAAAVNVIVTTPTQATITASGSTSFCSGGSVTLTASTGTSYMWSTGATS
                     NAITTSSPGTYTVTITNNGCQSTTNKVVTVYALPTATITANGSTNLQQGGSVQLTASS
                     AGSGGSYLWSTGATTQSITVSTAGNYTVKVTNSNGCSNTSAPMTVTVNSSFQPTITVS
                     GSLSFCSGGNVKLTATKGSSYLWSTGATTRKITVTTAGSYAVTVTQFTTGYVGTSSVV
                     NVNVMPAPSATITASGSTTICQGSSVTLTSSNGANYLWSNGSTSQSISASTAGNYIVT
                     VTGSNGCTKSSSATAVVVNNCGSSCPTPGNLHVTNLRPTSAEINWNLTSANNYYIKVE
                     NLNTGYVYYSNALSSGVNSITIGASPSTPYRWSVRGLCGSTYSSWSAYNQFTTPAARM
                     GEGNSNEGLNITPLTTQMPSELEEQPLRIESDDASVTFFPNPATSFVTFNVTASEKNT
                     ATLTLLDLSGRTLNKESYQFNEGLNNGKVDVSNYARGVYMIQLVFDGKTVMKKVILE"
     gene            5952..6491
                     /locus_tag="PK134_03165"
     CDS             5952..6491
                     /locus_tag="PK134_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRB37879.1"
                     /translation="MKFRTIGLMIFTLLYFGQSAKAQQSENIVEDNLRNYYSQILGYQ
                     VKEVFNTKVFDLIDTWLNTPYKYAGKNENGIDCSGFVNVIIKAVYGSSFGCSSVDMYN
                     NLPHVSLKNLTPGDLVFFKTRGRRISHVGMYLGQDKFVHSSTSSGVIVSDLNEPYYKK
                     RFAKGGRISLDESTTSVSK"
     gene            complement(6496..8130)
                     /gene="pruA"
                     /locus_tag="PK134_03170"
     CDS             complement(6496..8130)
                     /gene="pruA"
                     /locus_tag="PK134_03170"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRB37880.1"
                     /translation="MTNAVYNIPPAYNEPVLNYAPGSPEKMALKAALAEARSKQMDIP
                     MYIDGQEVRTGNTSALRPPHDHQHILGMFHKGEKKHVQLAIDAALRAKKQWAEMPWEQ
                     RAAIFLKAADLLAGPYRYKMNAATMLGQSKNAYQAEIDSACEMIDFLRFNVQYMREIY
                     TQQPPVVGKGVWNRTEQRPLEGFVFALTPFNFTAISGNLPTAPAMMGNTVVWKPANTQ
                     IYAANVVMQLLIEAGLPAGVINLVYVSGPDAGDVIFKHADFGGIHFTGSTEVFKGIWK
                     TIGENMPLYKSYPRIVGETGGKDFIIAHKSADIDVLVTALARGAFEYQGQKCSAASRC
                     YIPKSIFEEVKSKLVKEVKSMKLGPVEDFRNFVNAVIDEKSFDKISAYIENAKKSKSA
                     EIIAGGNFDKSKGYFIEPTIILTSDAKYTTMCEEIFGPVLTMYVYEDDKFEETLHLVD
                     NTSEYALTGAIISQDRYAVDVATRALVNSAGNFYINDKCTGAVVGQQPFGGARGSGTN
                     DKAGAMVNLLRWVSPRTIKETFVPAKDYRYPFMEAE"
     gene            complement(8218..>8772)
                     /locus_tag="PK134_03175"
     CDS             complement(8218..>8772)
                     /locus_tag="PK134_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB37881.1"
                     /translation="EATTALRKLNNNSIIIAMTAFALEDDKKQCLDAGMNDYTTKPIR
                     IEVVQSLLEKWGDVILKSKAARNDEPLIDKQTIERLKAMAVGDDTSFVKNIFELFLKQ
                     MDNLHADMSKFFNEHDADGMYKASHKLKGSALNIGAKRLSEVCRIIEEKGKLGDLTGM
                     NELIQQLAEVISLTKNEIRSLSLA"
BASE COUNT         2738 a         1745 c         1618 g         2671 t
ORIGIN      
        1 ttaacgaaca aacagatgcc acattaagag ggatgcttgg ttttgctaat ggcaaatatt
       61 ctaagcgtat atatcaatct tttgatggtg taaaaacatt acaggttaca gaaaatgctt
      121 cttctgttat agatatagca ctcaacttag gctttgaagt aaaatactct tttcataaaa
      181 acgttggagt tatgtttgct ggcgactatt tccgtacaga atttaactac gatgatgtgt
      241 atttaattta ttccgaaaac ggagaaaaaa ggtcaaccgg tgcttactca caacctgttg
      301 atgtttttca attttcagcc ggaattttta ccaaatttta atgggcgaaa gttcgctttt
      361 tttgggtgaa agtcattttt ttgatggcga aggttgagtt attaacaggt gttaataaca
      421 ctatgcgctg attatcatca ttttaacgta ctgaagtcga aaaggtcttt tttgggtgaa
      481 aattcgattt atttttggac gcgttaacca attcttctat tccgtttaac aacaaaattt
      541 attaatgcga aaaaacttcg gttaatcgca gggggcttat tatgtttaat ttctaattac
      601 catttcacat gattaaatca aatttaatct caattacaag gagcattgtt gcagtgttct
      661 tgttagcagt accaacttgt ttggttgctc aaaattatca gccaaagtcg tattatactt
      721 tcgaaggctc ctcgccaatg aaagattcga tgggcaattt taatcttgat ccgaactatt
      781 atcagtcaag ttatcaaata cttaccaata cttcaggtgg tgttggcaag tatatgaaat
      841 tggactcaag cagtaccatc atcagaggtg gtgtacttgc cattgattct gccataacat
      901 ttgagtttat ttttaaaccg ggcatgaact ttggcacaac agtatttgcc aagagaatgg
      961 atggcgcact tgaattgcgc atgacctatc cggcaattaa cttcatgaca gaagtaaaag
     1021 ctgctaatgg ctcaccaatt tatgatgatt ttgaaattga tcttaacggc attggccgtg
     1081 catcatacag ttattatgtt gatgacaact ggcatcatat tgtatttaag tataatgcta
     1141 aagccggtat taaagaggtt tgggttgacg gacaattacc tgcaggattt tcaaaaacca
     1201 tagcaggtgg tagtttctca cagagtggca acagagatta catgttgaac tcacaatcgg
     1261 attactataa gttgcagggt gcaattgatg agattgcagt ttaccacaac tcacttggtg
     1321 gcaattctat ttatgctcac tatttgaatt ttatggcggg caaacactat tcatttgcaa
     1381 acccaacggt tgttgcacct tcgccaagtc cggtaactgc aggaatcaac atcaatgatt
     1441 ttgcacccgg acatccatcg ccatctctta ctccaattga gcaattgaaa aactttccaa
     1501 caccaagata taagccagga catacattat ttccaaacaa tccgtttgta ggctactatt
     1561 atctttcagg atatcagact tatgaaactg tttattcaaa agctgtagca aacagtgtta
     1621 cactgcagac agattttgca aaaaacttta actacaccat tatggctgcc gaaaataccg
     1681 gtgcctatag tgattactca aactcaacta ctttttcaca tgcatggatt aatctagcaa
     1741 acagctatcc acaatataaa gtatctgcat tatcatattg gccacaattg cgcccgacag
     1801 atgcaggtta taaaagcaat agtccttatg caacttgtgg atgtttaccc gataagtatt
     1861 atttaaaaaa tagttccgga caatatctga atcctaatgg aggaacaggc tctacaaaat
     1921 atctgagtcc tgcaggtgca ccggactcat tggtaaaaga tggacttact cagaaatttt
     1981 acatgcaaca attacttgca aaattgagca gacctataga ttatatatta gataatggtg
     2041 aagtcgttcc ttttttatat tacaccaacg ttatgcaatc tgatccaagt gttgtttcag
     2101 ataaaaattc atcagggctt gattggttca cttatgaagg tcgcagagca gcaacaaaaa
     2161 taaatgctta tcgcgatcag ttcatgggct taagcggttt atcgaataca aaatttgctt
     2221 attatcagtt agacggtcag ccgacatgga atctgaaatg gtctgaagta cgcacagtga
     2281 atacagccat gaatggttca cactatgcaa ccggtgatat ttatatgcgt tggccatcta
     2341 actggcgcta ttggagtggt gcatggaaag gatggcaatg ggttgttgaa tcaagaagag
     2401 agcaattatt agcgggagac aaatattttt cacctgctgt ttcaccggga tgggacaatg
     2461 acgaaacaca aaacgttcgt cctgcacaat ggttaggctt tcttaaagct gtttcaatga
     2521 caggtgcaga atttttcaca acaggttatt tcgtaacgtc tcaaccttat cagaatccaa
     2581 aaaattatat ctggcaattg gttattcctc cttatgcaca agcaatcaca agccgttacg
     2641 aggacttatt cagaaacgga tatctgatgt cgggcgatgt gcctaacagt acaacaagtc
     2701 caacttacat ggcttactct ttcaacactg gcgaccccgg aaaattagtt gttgtacgta
     2761 aacacaattc acaaaataaa tatgccatca caggttcatc acaaccaatg tctaatatgg
     2821 caggtgctgc acctgatgaa gctgttgcac aaattaattt agatggcagt agaattaaat
     2881 ttaaaatcag aagacaaggt agtacatata tttatgataa caccaacact tctgcaccaa
     2941 tattctatca gttagatgga tggcatgaaa atacttatcc atattattgg tcaaaagatt
     3001 ttaaatttga agcagagtta aatgacaaca gcaacagtaa tgtatatctg aaaacaaccc
     3061 gtcctgcagg agcagcagca ggcgacttta cagatttcac cactgctgta ggttttaatt
     3121 ctgtaagtga tttaacttac aacttccagc caagatctac aacagactat acgtattatg
     3181 tttgggttag agcacgttct aaaaactcac aaactacagg aatgacaatt tccttagatg
     3241 gtagcaacag caaacaaatt ggttgtatta cagatgctaa ttggcaatgg tataaatatg
     3301 atgtaaactc aggcgcagta atttcattca cctctgtaac atcattagca gatcacattt
     3361 taaaaattac accaaccaat actaatcttg agattgacca gattcatttg tcaaccagca
     3421 gcaatgctat ttatacaagc cctgcaccct gttcaggtgt tgcagtgcca acaatcagtg
     3481 caagcggacc tgttgcgttc tgtgcaggag gaaatgtaaa tcttattgcc agtaatggtc
     3541 tctcttatct atggagcaat ggttcaacag atccatatat taatgtaacc agtgcaggtt
     3601 cgtatgttgt tactgttaca actgctgcag gaacagcaac atcagcacct gttacagtga
     3661 cagtttatgc acaaccaaat gcaacaatca ctgcaaatgg ctcaacttct ttttgtgttg
     3721 gcggaagtgt tactctcact gcaagcagcg gctcaacata tttgtggagc agtggtgcaa
     3781 ctacacagtc aattacagct attggaggcg gaaactacaa tgttatagta acaaactcta
     3841 atggttgttc tgcaatgtct gcatcaacag cagtaacagt taagccagtt cctactgcca
     3901 caatttcacc aagtggtcca actacttttg ctgccggtgg aagtgttaca ttaacttctt
     3961 cttcagcagg taccggtgga agttacaaat ggtcaccggg tggtatgaca acacaagcag
     4021 ttacagttag cacaaccgga aactatgttg taactgttac taacaacagt ggttgttctg
     4081 caacatcatc accattatca gttactgtaa cttctacagc accaacacca aaaattacag
     4141 ctaacggacc aacaactttc tgtcagggtg gaagcgtagt attaacttgc agtgcagcaa
     4201 attcatatct gtggagtaat ggtgcaacaa cacaatctat tactgttacc acagcaggaa
     4261 gttatacatg tacagcaaca acagcaggag gttccgcaac agcagcagca gttaatgtaa
     4321 tagtaacaac acctactcag gcaactatta cagcatcagg aagtacttcg ttctgtagtg
     4381 gaggtagtgt aacgttaaca gcaagcacag gaacttcata tatgtggagt acaggtgcaa
     4441 cttctaatgc aattactacg tcatctccgg gtacatatac agtaacgatt acaaacaatg
     4501 gttgtcagtc aacaacaaat aaagtagtaa ctgtttatgc cttgccaact gcaacaatta
     4561 cagcaaatgg ttcaacaaac ttacagcaag gtggaagtgt tcaacttacc gcctcaagtg
     4621 ccggtagtgg tggaagttat ctatggagta caggtgcaac aacacaatca atcactgttt
     4681 caacagcagg aaattataca gttaaagtaa caaacagcaa tggttgtagt aatacatcag
     4741 cacccatgac tgtaacagta aattcatcgt ttcaacctac aataactgtc agcggttcat
     4801 tgagcttttg ttccggtggt aacgtaaaat taacagcaac taaaggttcc tcttatttat
     4861 ggagtacagg ggcaacaacg cgtaaaatta cagttaccac agccggaagt tatgctgtaa
     4921 cggtaacaca atttacaaca ggttatgtag gaacatcatc agttgtaaat gtcaatgtaa
     4981 tgcctgcacc ttcagcaact attaccgcaa gtggcagcac cacaatctgt cagggatcaa
     5041 gtgttacgtt aacctcaagc aatggtgcta attacctttg gagtaatggc tctacatcac
     5101 aatctatttc tgcatctact gccggcaatt atattgttac cgtaacagga agtaatggtt
     5161 gtactaaatc atcatcagct actgctgttg tagttaacaa ttgtggttct tcttgcccaa
     5221 cacccggaaa tttacatgta acaaacctca gaccaacgtc tgcagagata aactggaatc
     5281 tgacttctgc aaataactat tacattaaag ttgaaaatct gaataccgga tatgtttatt
     5341 attctaatgc attatcaagt ggtgtaaact caattaccat tggagcatca ccatcaactc
     5401 cttatcgttg gtccgtacgt ggattgtgtg gcagcacata ctcttcatgg agtgcttaca
     5461 atcagtttac aactcccgca gcaagaatgg gcgaaggtaa tagtaatgaa ggtttaaata
     5521 ttactccatt aactacacaa atgccgtcag agttagaaga acaaccactt cgtattgaat
     5581 cggatgatgc aagcgttact ttcttcccta atcctgcaac ttcatttgta acatttaatg
     5641 tgactgcatc tgaaaagaac acagcaacat taacgctgtt agatttgtca ggacgcacat
     5701 taaataaaga aagctatcaa tttaacgaag gactaaacaa cggtaaagtt gatgtgagca
     5761 attatgcaag aggtgtttat atgattcaat tagtttttga tggaaaaact gtgatgaaaa
     5821 aagttattct tgaataattg gtttttatta tcataagtgt taagagccgt tatcagattg
     5881 atgacggctc tttttcatct tagacaatac ctgctaaaag aatgcgttca ttaaaatgtt
     5941 atctttgccc aatgaagttt cgcacaatag gtttaatgat ttttacattg ttgtattttg
     6001 gacaaagtgc caaagcgcag caatctgaaa atattgttga agacaacctg cgaaattact
     6061 acagtcagat tttgggctat caggttaagg aagtatttaa cacaaaggtt tttgatctta
     6121 tagacacttg gctgaatact ccatataaat atgccggcaa aaacgaaaat ggaattgact
     6181 gttccggatt tgttaacgtt atcatcaaag ctgtttatgg cagttcattc ggttgcagct
     6241 ctgttgacat gtataacaat ctgccacatg tttcgcttaa aaatttaact ccgggtgatt
     6301 tggtattttt taaaacccgt ggacgaagaa tttcacatgt aggtatgtat ttgggtcagg
     6361 acaaatttgt acactccagc acatcaagtg gtgttattgt aagtgacctt aatgaacctt
     6421 actataaaaa aagattcgcc aaaggaggaa gaatttccct tgacgaatct actacttccg
     6481 tgtctaaata aatttttatt ccgcttccat aaaaggatat cggtaatctt ttgccggaac
     6541 aaaagtttct tttatcgttc gtggtgatac ccaccgcaac aaatttacca tagcacctgc
     6601 cttgtcatta gttcctgaac ctctggcacc accaaatggc tgttggccaa caacagcacc
     6661 tgtacattta tcattaatat aaaagttacc tgcagaattt accaatgctc gtgttgcaac
     6721 atcaactgca tatcggtctt gagaaataat tgctcctgtt agagcatact cgctggtgtt
     6781 gtcaactaaa tgcaaagtct cttcaaactt atcatcctca taaacataca tagtcaacac
     6841 ggggccgaaa atttcttcac acatggttgt atattttgca tcggatgtaa ggataatggt
     6901 tggctcgata aaatatcctt tgctcttatc aaagtttcca cctgcaataa tctctgcact
     6961 cttactcttc tttgcatttt caatgtaagc agaaatttta tcgaatgatt tttcgtctat
     7021 aacggcattt acgaagttgc ggaaatcttc aaccggtcca agtttcattg actttacttc
     7081 tttaaccagc ttgcttttga cctcttcaaa aattgacttc ggaatataac aacgtgatgc
     7141 agcactgcat ttctgacctt gatattcaaa agctccacgt gccaatgcag tcactaatac
     7201 atcaatatct gcactcttgt gcgctataat aaaatctttc cctcctgttt cgccaacaat
     7261 tcggggatat gacttgtaca aaggcatatt ttctccgata gttttccaga ttcccttaaa
     7321 aacttctgtt gatcctgtaa aatgaattcc accaaaatca gcatgtttaa aaataacatc
     7381 acctgcatcg gggcctgaaa cataaacaag attgattact cctgcaggta aaccggcttc
     7441 aatcaacaat tgcattacca cattcgctgc ataaatttgt gtattggcag gtttccaaac
     7501 aacagtgtta cccatcattg ccggtgctgt aggcaaatta cctgaaatgg ctgtgaaatt
     7561 gaatggcgtt aaagcaaaaa caaatccttc gagtggacgc tgttcagtac ggttccatac
     7621 acccttacct actacaggtg gttgttgtgt ataaatttca cgcatatatt gtacgttgaa
     7681 acgcaaaaag tcaatcatct cacatgctga atcaatttct gcctgataag cattttttga
     7741 ttgtccaagc atggtagcag cattcatctt atagcgataa gggcctgcaa gtaagtcagc
     7801 tgcttttaga aaaattgctg cacgttgttc ccaaggcatt tctgcccatt gctttttagc
     7861 cctaagtgct gcatcaatcg ctaactgcac atgcttcttt tctcctttat gaaacatgcc
     7921 cagaatatgt tgatgatcgt gcggagggcg aagcgctgaa gtatttcctg ttcgtacctc
     7981 ttgcccatca atatacatgg gtatatccat ttgtttggaa cgggcttctg caagtgctgc
     8041 ctttaatgcc attttctccg gacttccggg tgcataattt aacaccggtt cattataggc
     8101 cggaggaata ttataaactg cgtttgtcat accttaatta atttaataat gtgcaaaggt
     8161 acgaaaatga aagaagggct tttgtatcct tggttacatc aacaaaaagt ttaaaaatca
     8221 agccaaactt aaactcctga tttcattctt tgttaatgaa ataacctctg ccaactgctg
     8281 aatcaattcg ttcattccgg taaggtcgcc aagttttcct ttttcttcaa taattcggca
     8341 aacttcagat aacctttttg caccaatatt gagagcgctt ccttttaatt tatgcgaagc
     8401 tttatacata ccatcggcat catgctcatt gaaaaattta ctcatatcag catgtaagtt
     8461 atccatctgt ttaagaaaaa gttcaaatat attttttaca aaagaagtat catctcctac
     8521 ggccattgcc ttaagtcttt caatggtttg tttgtcaatt aaaggctcat catttcgtgc
     8581 agccttactt tttaaaatca cgtcacccca cttttccaat aatgattgta caacttcaat
     8641 tctgattggc ttagtcgtgt aatcattcat gcctgcatca agacattgct ttttatcatc
     8701 ttccaatgca aaagctgtca tggcaataat aatgctgttg ttatttaact tacgcaatgc
     8761 tgtcgttgct tc
//
LOCUS       DAORQT010000051         8692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1132_length_8692_cov_7.979044, whole genome shotgun sequence.
ACCESSION   DAORQT010000051 DAORQT010000000
VERSION     DAORQT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_8692_cov_7.979044"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3016
                     /gene="mfd"
                     /locus_tag="PK134_03180"
     CDS             <1..3016
                     /gene="mfd"
                     /locus_tag="PK134_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRB37882.1"
                     /translation="QSFIVVSYAEAVAENVVTRHALEKNTIEARVNEQLSIDFVNDFL
                     TENNFQRTDFVFEPGQFAIRGGIVDVYSYAYELPFRLEFDGNKIESIRSFDPVTQLSE
                     KNLHFVTIIPNIQTEMQQNVTESFFKFLPENTLIWTRDLSYSLNYLDDNLTVLKNQTS
                     ENDEEEKSDNPRIVYDSIEEIKSVLSTFRQVEFGLKSFYENVVGIKFNIQPQPQFNKN
                     FNLLAGHLKENSDKHFKSYIFSDSAKQVERIYAIFDDLSFKKEVQRDDIQFSPVYTSL
                     YKGFTDLDLKVSVFTDHQIFDRYHRFKLRKNYSRNEAISIKELYSLKPGDYVTHIDHG
                     VGRFGGLEKLNIEGRQQEAIRLVYKDNDILYVSIHSLHRIARYTGKEGTAPSLNKLGS
                     TSWASLKQKTKKKVKDIARDLIALYAKRKAQEGFAYTPDNYLQTELEASFIYEDTPDQ
                     IKATRDVKKDMESASPMDRLICGDVGFGKTEIAIRAAFKAVNDSKQVAILVPTTILAM
                     QHYRTISERLKDFPCTIDYINRFKSTAQQNETLRKLEVGKIDIIIGTHRLLSKDIKFS
                     NLGLLIIDEEQKFGVASKEKIKSLKVNVDTLTLTATPIPRTLQFSLMGARDLSIINTP
                     PPNRYPVTTELHTFNDKVIREAIMYEVSRGGQVFFVHNRVQDIHDISDMIRKLCPDVK
                     IVVGHGQMDGSQLEEVLMTFIEGDADVLVATTIIESGIDISNANTIIINQAQNFGLSD
                     LHQMRGRVGRSNKKAFCYLLAPPLTVLTPEARQRLQAIEEYSDLGMGFQVALRDLDIR
                     GAGNLLGGEQSGFISEIGFEMYQKILDEALIELRESDFKELFPADDNKPIVADCQIET
                     DLEILIPTQYVENIVERLNLYKELDDIETEEKLNAFESQLRDRFGPVPKQVMELFETI
                     KLRWKAKSLGFEKLILKNGQLKGYLVSNPDSRLYKSDLFMQIINFVKSNPSRCRMKED
                     KSKLSLTFYKIETVNEANAVFRQMMKPSVATA"
     gene            3047..3118
                     /locus_tag="PK134_03185"
     tRNA            3047..3118
                     /locus_tag="PK134_03185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3080..3082,aa:Arg,seq:ccg)
     gene            complement(3195..8156)
                     /locus_tag="PK134_03190"
     CDS             complement(3195..8156)
                     /locus_tag="PK134_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB37883.1"
                     /translation="MYQHPDIFSLTKFLTNGSATKSFYENAQDRFSSNIKGKIETVED
                     GIAVIGMAGRFPGANNVDDFWKNIVEGKETVSFFKKQELDNDVPEEDKNDENYVAVRG
                     IIEDADKFDAPFFNMNPRLAEVTDPQQRIFLQVAWEAIENAGYAPDKYDGLIGVFAGM
                     GNNTYFPNNVFHNKEAINRVGSFQTMTANEKDYIATRIAYEFNTKGPAVSVHTACSTS
                     LTAVTLAYESLLNHECDMALAGGVAITVPVNSGHIYNEGGMFSKDGHTRAFDAGANGT
                     VFSDGAGAVILKRYKDAIKDGDEIYAVIRGAALNNDGSEKASFTAPSVEGQAAVIAMA
                     QAKAGVSPDSISYIETHGTATPLGDPIEIEALTQAFRSKTDKKQFCAVGSVKTNIGHL
                     TPAAGVAGLIKTCLALKNKILPASLNYSKANAAIDFKSSPFYVNSTLKKWTTENGEPL
                     RAGISSFGVGGTNAHVVVEEAPQKAESGIARKKHLFLLSGRTKDVLDSQTKNLQSFLE
                     KNPDANLADIAYTLQTGRKYFNHRRIISGGDVQEIVSAIQQANPKKTAARELTTGNPE
                     IIFMFPGQGSQYVNMGKNLYDDEIVFKDAVDTCCNILQPHLGIDLRTILYPEDAEKEK
                     AEKLLKETIYTQPSLFTIGYALAKLWMSWGIQPKGFIGHSIGEFAGAHFAGVFSLEDA
                     LMLVANRGRMMQELPHGSMLSVRKAAAEIEKEIDSSCSIAAINGPALCVVAGPAEKIE
                     ALQKSLESREITAKLLVTSHAFHSPMMEPMLKPFLEKVKSVKLNKPAIPFVSTATSKW
                     ITDAEATNPEYWMNHVRATVRFAEGIQTLWGDKPQRVLLECGPRNTAATLAKQQAKEP
                     AKQTAISSLSDSADNYAEWEQILFALGQLWMSGVAIDWQNFYALEKRNRIALPTYPFE
                     KKRYWLDPINVIQNITQENIQPSVSGFEFPIITQPSVNTTIETTNNTTMNRKDLITAE
                     LKTILEESSGMELGNDSSATFLDLGLDSLFLTQAALTISKKYGVKVTFRQLNEEFSSL
                     QSLSAHLDANLPADAMPAAKLAPQQAPQQTNIVMQQPAMQTTGMNPMQMMMMQQMQMM
                     QMMMQQMNQPQQQVAPSVQAHIEKAVEIKSHTKKEDVSENEKAELAKPFGAIARIEKG
                     TSNDISSEQEKWLKQFIAAYTEKTKASKKYCQDYRSVLADPRVVTGFKPALKEIIYQV
                     VVNKSKGVNFHDIDGNEYVDVLNGFGSNFFGWGNDMLKNAWKKQLDDGVELGPQTPLA
                     GECAQLICELTGYDRAGFCNTGSEAVLGAMRIARTVTGRNLIICFNGSYHGINDEVIV
                     RGTKKLKSFPAAAGIMPESVQNMLVLDYGTEESLQIIRERMDEIAAVMIEPIQSRRAD
                     FHPKEFIHEVRKLTKENNVLMIFDEVITGFRLRLGGAQEYYGVDADLSTYGKVIGGNM
                     PVGAIAGKKQYMDALDGGFWQYGDNSIPEVGVTYFAGTFVRHPLAMAAMKEVLLHLKK
                     EGKPLYEKLNAKTQRLVDEVNAHSKKLGSPFKLVTFGSLFKGKWETEPHYTELLFAMM
                     RYKGVHIMDGFPCFLTLAFTDADVDFVVSKFKESLSELCEIGFIPSDKNNEQSKINSN
                     GHLQNGNGQHPPIPGARLGKDANGKDAWFIADPERPGKFLMVK"
     assembly_gap    8216..8249
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8250..>8692)
                     /locus_tag="PK134_03195"
     CDS             complement(<8250..>8692)
                     /locus_tag="PK134_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HRB37884.1"
                     /translation="GNIEYLGRIDGQVKVRGYRIELGEIELTLDKHATVKQAVVIARE
                     DEPGDKRLVAYCLPHDNKKILAGELRQFLQDKLPEYMMPSAFVQIEEFPRTPSGKIDR
                     RSLPAPSNKRPDIGTVFVAAETDLQKTFVKTWSKLLKVAEIGIND"
BASE COUNT         2546 a         1687 c         1623 g         2802 t
ORIGIN      
        1 tcagtcattt attgttgtat cgtatgccga agctgttgca gaaaatgttg taacacgaca
       61 tgcactcgaa aaaaatacta ttgaagcgcg tgtgaatgag caactgagca ttgattttgt
      121 aaatgatttt ctgacagaga ataattttca aagaactgat tttgtttttg aacccgggca
      181 gtttgccatc agaggcggta ttgttgatgt ttattcttat gcctatgaac tgcctttcag
      241 attagagttt gatggaaata aaattgaatc tattcgctca ttcgatccgg tgacacaatt
      301 gtctgaaaaa aatctgcact ttgttaccat cattccaaac attcagacag aaatgcagca
      361 gaatgtgaca gaatcgtttt ttaaatttct tcctgaaaat actttaatct ggacacgcga
      421 tctttcttat tcattaaact atcttgatga taatttaaca gtgcttaaaa atcagacttc
      481 tgagaatgat gaggaagaaa agagtgataa tccaagaatt gtttatgatt ctatcgagga
      541 gattaaatct gttctgtcaa ctttcagaca ggtagagttt ggtttgaaat ctttttatga
      601 aaatgtagtc ggaataaaat tcaatataca gccacaacca cagttcaata aaaacttcaa
      661 tttacttgcc ggtcatttaa aagaaaatag tgataagcat tttaagtcat acattttttc
      721 tgattctgcc aaacaggttg aacgcatcta tgccattttt gatgatttaa gttttaaaaa
      781 agaagttcag cgtgacgata tacaatttag ccctgtttat acttcattgt acaaaggttt
      841 tactgattta gatcttaaag tttctgtatt cacggatcat cagatttttg atcgctatca
      901 tcgttttaaa ctacgtaaga attacagccg aaacgaagcc atttccatta aagaattgta
      961 cagcctcaag cctggtgatt atgtaacaca tattgatcat ggtgtaggtc gttttggtgg
     1021 attggaaaaa ctaaatatag aaggtcgtca gcaagaggca atcagattgg tgtataaaga
     1081 caacgatatt ctttatgtaa gtattcacag cctgcatcgc atagcccgct acacaggtaa
     1141 agaaggcact gcaccatcgc tcaataagtt gggtagcaca tcatgggcat cgctcaagca
     1201 aaaaacaaaa aagaaggtaa aagatattgc gcgcgatctg atagcactat atgccaagcg
     1261 aaaagcacag gaaggctttg catacactcc cgataattat ctgcaaacag aattagaggc
     1321 aagttttatc tatgaagata cgcccgatca gataaaagca acacgtgatg taaaaaagga
     1381 tatggaaagc gcatcgccaa tggacagatt aatttgtggt gatgttggat ttggaaaaac
     1441 agagatagcc attcgtgcag cttttaaagc cgtaaacgat agtaaacagg ttgccatttt
     1501 agtgcctact accatattgg caatgcagca ttacagaacc attagcgaaa ggttgaaaga
     1561 ttttccctgt actattgatt acatcaaccg ttttaagtca actgcacaac aaaatgaaac
     1621 actacgtaaa ctcgaagtag gtaagattga catcatcatt ggcacgcaca ggctgctgag
     1681 caaggacata aaattcagta atcttgggtt gttgattatt gacgaagagc agaaatttgg
     1741 tgttgcaagt aaagagaaaa ttaaatctct taaagtaaat gttgacacac tgactttgac
     1801 agcaacacct attccaagaa cacttcagtt ttcattaatg ggtgctcgtg atttatcaat
     1861 cataaacaca ccgccaccaa accgctatcc tgtaacaaca gagttgcata catttaatga
     1921 caaagttatt cgtgaggcaa taatgtatga agtatcgcgt ggaggtcagg tgttttttgt
     1981 gcacaatcgt gttcaggata ttcacgatat ttctgatatg ataagaaaac tttgtcctga
     2041 tgtaaaaatt gttgtagggc acggacagat ggatggcagt cagcttgaag aagtgcttat
     2101 gacttttatt gaaggtgatg cagatgtttt ggtggcaaca accatcatcg aatccggaat
     2161 tgatatcagc aatgcaaaca caattatcat caatcaggca cagaattttg gattgagcga
     2221 tttgcatcag atgcgtggca gggtaggacg aagtaataaa aaagcttttt gttatctgct
     2281 tgcaccacca ctcactgtgt taacaccaga ggcacgtcag cgactgcagg ccatagaaga
     2341 atattccgat ttaggaatgg gatttcaggt agcgttgcgc gacttagata ttcgtggtgc
     2401 aggaaattta ctgggtggcg aacagagtgg ttttatttct gaaataggat ttgaaatgta
     2461 tcagaaaatt ttagacgaag cgttgataga attgcgcgag tctgatttta aagaattatt
     2521 tcctgctgat gataacaaac caattgttgc cgattgccag attgaaactg accttgaaat
     2581 tttgattccc acacaatatg tagaaaatat tgtggagcga ttgaatcttt acaaagagtt
     2641 agatgatata gaaacagaag aaaaactgaa tgcttttgaa agtcagcttc gtgacagatt
     2701 tggccctgta ccaaagcaag taatggagtt gtttgaaact attaaactgc gatggaaagc
     2761 aaaatcatta ggttttgaaa aactaatttt aaagaatggg cagcttaaag gatatttagt
     2821 ttcaaatccc gattcacgat tgtataagag tgatttattt atgcagataa tcaattttgt
     2881 taaaagcaat ccatcgcgct gccgcatgaa agaagataaa agtaaactta gtcttacctt
     2941 ttacaagata gaaactgtga atgaagctaa tgctgttttc cggcagatga tgaaaccatc
     3001 agtggcaacg gcataggttg attattgtac atttgcagcc ttgtatggtt ctgtagtata
     3061 atggatagta cgcaggtctc cggaacctga gattcgagtt cgactcccga caggaccact
     3121 tagatttcat attgatatta aagaagtatc aatagtaacc gtttttctat tggattgcta
     3181 aaagcaaaat aatattactt caccattaaa aacttacccg gtctttctgg gtcggcaata
     3241 aaccaggcat cttttccatt agcatctttt cctaaacgag cgcccggtat tggaggatgt
     3301 tgtccattgc cattttgcag atgtccgtta gaatttattt tcgattgttc gttgttttta
     3361 tcagaaggaa tgaatccgat ttcgcaaagc tcagataaac tttctttgaa tttagaaaca
     3421 acaaagtcaa catctgcatc tgtaaaagca agtgttaaaa agcatggaaa gccatccatg
     3481 atgtgaacac ctttatagcg catcattgca aaaagtaatt cagtgtaatg aggctctgtt
     3541 tcccatttgc ctttaaataa agagccaaag gttaccaact taaatggtga gcctaatttt
     3601 ttactgtgtg catttacttc gtcaaccaat ctttgtgttt tagcattcaa cttttcataa
     3661 agcggcttgc cttctttttt aagatgcaac aacacttctt tcattgctgc catagcaagc
     3721 ggatgacgta caaatgttcc agcaaaatag gttacaccaa cttcaggaat agaattatca
     3781 ccatactgcc agaatccacc atctaaagca tccatgtatt gtttcttacc tgcaatcgca
     3841 ccaacaggca tattgccgcc aataactttt ccgtaagtac tcaggtcggc atcaacacca
     3901 taatattcct gcgcaccgcc aagtcttaat ctgaatcctg ttatcacctc atcaaaaatc
     3961 atcagcacat tattttcttt agttagtttt ctaacctcat gaataaattc tttaggatga
     4021 aaatctgctc tgcgtgattg aataggttca atcattacgg cagcaatttc atccatgcgc
     4081 tcgcggataa tctgcaaact ctcttctgtg ccataatcta atacaagcat attttgtaca
     4141 gactccggca taattcccgc tgctgccgga aacgatttta gtttttttgt tccacgaaca
     4201 atcacttcat cattgatgcc atgataagaa ccattgaaac aaataataag atttcttcca
     4261 gtaacggtac gtgctattct catagcaccg agtacagcct cgctgcctgt gttgcaaaaa
     4321 cctgcacggt catatccggt gagttcacaa attaattgtg cacactcgcc tgcaagcggt
     4381 gtctgtggac caagctcaac gccatcatcc aattgttttt tccatgcatt ttttagcatg
     4441 tcgtttcccc aaccaaaaaa gttggagcca aaaccattca acacatctac atattcattg
     4501 ccgtctatat cgtgaaagtt tactccttta gatttgttaa ctacaacctg ataaattatt
     4561 tctttcaaag caggtttaaa tcctgtaacc acacgtgggt cggcaagcac actgcgataa
     4621 tcctgacaat atttttttga agcttttgtt ttttcagtgt atgctgctat aaattgtttc
     4681 aaccattttt cctgctcaga tgatatgtca tttgatgtac ctttttcaat tctggcaata
     4741 gcgccaaatg gttttgccaa ttcagctttt tcgttttcgc ttacatcttc tttcttagtg
     4801 tgcgatttta tctcaaccgc tttctctata tgcgcctgaa cagaaggtgc aacttgttgt
     4861 tgtggctgat tcatctgttg catcatcatc tgcatcattt gcatttgctg catcatcatc
     4921 atctgcatgg ggttcatgcc tgttgtttgc attgccggtt gttgcatgac aatatttgtt
     4981 tgctgcggtg cttgctgcgg tgccaattta gctgcaggca ttgcatctgc tggtaggtta
     5041 gcatctaaat gcgctgaaag cgattgcagt gatgaaaact cttcgttaag ttgtctgaat
     5101 gtaactttta ctccatattt tttgctgatg gtcagggcag cctgtgtcag aaaaagtgaa
     5161 tccaatccta aatcaaggaa ggttgcactg ctgtcgttgc ccaattccat acctgaagat
     5221 tcttcaagaa ttgtttttaa ctctgctgtg ataagatctt ttctattcat tgttgtattg
     5281 ttcgttgttt caattgttgt gtttacagat ggctgagtaa tgattggaaa ctcaaagccg
     5341 gaaactgatg gttgaatgtt ttcttgtgta atattttgaa tgacattaat tgggtcaagc
     5401 caatatctct ttttttcaaa agggtaagta ggtagtgcaa tgcgatttcg tttttcaagt
     5461 gcataaaaat tttgccagtc aattgcaact ccgctcatcc acagttgacc taaggcaaag
     5521 agtatttgct cccattctgc ataattatct gctgaatcac tcagcgatga aattgctgtt
     5581 tgtttggcag gttcttttgc ctgctgttta gctaaggttg cagcagtatt tcttggtcca
     5641 cattccaaca aaacacgttg tggtttatca ccccacaaag tttgaatacc ttcagcaaaa
     5701 cgtacagtag ctcttacgtg attcatccag tattccggat tggttgcttc tgcatctgta
     5761 atccattttg aggtggctgt tgatacaaat ggtattgcag gtttattgag tttaacggat
     5821 tttactttct ctaaaaaagg tttcaacatg ggctccatca ttggcgaatg gaatgcatgt
     5881 gatgtcacta ataactttgc tgtaatttcg cgactttcta atgatttttg aagtgcttca
     5941 attttttcag cagggccggc aactacgcaa agtgcagggc cgttaattgc agcaatagaa
     6001 caagaagaat caatctcctt ttcaatttcc gcagcagcct tacgaacact taacatactt
     6061 ccatgtggta attcctgcat cattcgtccg cgattggcaa caagcatcag tgcatcttct
     6121 aaagaaaata ctcctgcaaa atgtgcacct gcaaattctc caatgctgtg accgataaat
     6181 cccttgggtt gaatacccca actcatccaa agttttgcta atgcatagcc gattgtaaac
     6241 aacgatggct gcgtataaat agtttctttc aaaagctttt ctgctttttc tttttcagca
     6301 tcttcaggat aaagaattgt ccgcaagtca atacctaaat gtggttgcaa aatgttacag
     6361 catgtgtcaa cggcatcttt aaaaactatt tcatcatcat ataaattttt tcccatgttt
     6421 acatactgtg aaccttgtcc gggaaacata aaaattattt caggattgcc tgttgtcagt
     6481 tcgcgtgcag ctgttttctt tggattagct tgttgtattg ctgatacaat ctcttgaaca
     6541 tcaccacctg aaataattct gcgatgatta aaatattttc gtcctgtttg taaagtgtaa
     6601 gcaatatctg caagatttgc atccggattt ttctctaaaa acgattgtag attttttgtt
     6661 tgtgaatcca atacatcctt agttcttcct gaaagtaaaa acaaatgttt ttttcgggca
     6721 atgcctgatt cagctttttg aggtgcttct tcaactacta catgagcgtt agttccgcca
     6781 acaccaaaag aactgatgcc tgcacgcaaa ggctcaccat tttctgttgt ccattttttt
     6841 agggtggaat taacatagaa tggagaagat ttaaaatcta ttgcagcatt tgcttttgaa
     6901 taattcagtg aagcaggtag tattttattt tttagtgcaa gacatgtttt aattaatcct
     6961 gcaacgcctg ctgccggtgt caggtgtccg atattggttt ttactgagcc aacggcacaa
     7021 aattgttttt tatcagtttt gcttctaaat gcctgagtga gtgcttcaat ttctattggg
     7081 tcaccaagtg gtgtggcagt accatgtgtt tcaatatatg aaatggagtc aggcgacaca
     7141 cctgcttttg cctgtgccat tgcaattacc gcagcttgtc cttcaacact gggtgcagta
     7201 aaacttgctt tttcactgcc atcattattt aaggcagcac cacgaattac tgcgtaaatc
     7261 tcgtcaccgt cttttatggc atctttatat cttttcagga taacggcacc tgcaccatca
     7321 ctgaatacag ttccgttggc acctgcatca aaagctcttg tatgtccgtc tttactgaac
     7381 atcccacctt cattatagat atgtccactg tttacaggga ctgtaatagc tacacctccg
     7441 gccaatgcca tatcacattc atggtttaac agactttcat aggcaagtgt aactgcagtg
     7501 agcgatgttg aacatgcagt atgcacactc acagccggtc cttttgtatt aaactcataa
     7561 gctattcttg tagcaatata atctttttca tttgcagtca ttgtttgaaa tgagcctaca
     7621 cggttaattg cttctttatt atgaaaaaca ttattcggaa aataggtgtt gtttcccatt
     7681 ccggcaaaaa ctcctattag tccatcatat ttgtcaggag cataccctgc attttcaata
     7741 gcttcccatg ccacctgtaa aaaaatgcgc tgttgcggat ctgtaacttc tgcaagtcgc
     7801 ggattcatat taaaaaacgg agcatcaaat ttgtcggcat cctcaattat acctcgaaca
     7861 gccacgtaat tttcatcgtt tttatcttct tccggcacat cattatctaa ttcctgtttt
     7921 ttgaaaaaag atacagtctc tttgccttca acaatgtttt tccagaaatc atcaacattg
     7981 tttgcaccgg gaaaacgccc tgccatacca attacagcaa tgccatcttc tacagtttca
     8041 atcttgcctt taatgtttga agagaatctg tcctgtgcat tttcataaaa tgattttgtt
     8101 gcagacccgt tagttaaaaa ttttgtcaga gaaaaaatat caggatgctg atacatttta
     8161 acaacaggaa gatctaaatt atgctcctgc cttagttctg ctataaattt taatgnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcattaatac caatctcagc aacctttaat
     8281 aacttactcc atgtcttgac aaaagttttt tgcaaatctg tttctgcagc aacaaataca
     8341 gtgcctatat ctggcctttt attagatggt gcaggtaaac ttctgcggtc aatttttcct
     8401 gaaggtgtgc gtggaaattc ttctatctgt acaaatgcag atggcatcat gtattcaggt
     8461 aatttatctt gcaggaattg acgaagttct cctgctaaaa ttttcttatt gtcatgtggg
     8521 agacagtatg caactaagcg tttatcaccc ggttcatctt ctcgtgcaat tacaacagct
     8581 tgttttactg ttgcatgttt atcaagagtt aattcaattt ctcccaattc aattctgtag
     8641 ccacgcactt tcacctgacc atcaattctt ccgaggtatt ctatattgcc at
//
LOCUS       DAORQT010000052         8561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1161_length_8561_cov_5.352104, whole genome shotgun sequence.
ACCESSION   DAORQT010000052 DAORQT010000000
VERSION     DAORQT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1161_length_8561_cov_5.352104"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1082)
                     /locus_tag="PK134_03200"
     CDS             complement(360..1082)
                     /locus_tag="PK134_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37885.1"
                     /translation="MKKLIIPILCIAILAFTSCKKKDKDNPHYTCSSCVTQAEALAAN
                     DGSSKGVYKGIIIGSSGTIKFSIQNGTNDITATMVVDGVSATLTSTVSWVSGQPYIAP
                     FTGTLNGQAVSITFSVQNNGSTPQIISSNIPGHPNAVFTIIKETSNALVEGFEGTYST
                     TEPESGTFNILLSRPLGQWGGISRENGQTSTNDVSGTITSSGDLYDDNNIKVGTLSGD
                     EIDGTFTDNSGNTVTVDGQRTL"
     gene            complement(1116..2738)
                     /locus_tag="PK134_03205"
     CDS             complement(1116..2738)
                     /locus_tag="PK134_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37886.1"
                     /translation="MKQMLPKVTAVWLASAILSPTLLIAQGNETTHELSKPAQKGILA
                     NAELSADGNIRMTYKMKVDKKSDQVNYEDYVFDKNLKFIGVEKTKEDKQTQPDQKVTL
                     IKAFVGGTNSFNVMSMSLNLQQEVWERNWNFDKQKYQWGSRISKEVVKPKNNDSKYRG
                     FAAYGNDDDGSVLILASYDSGEDDQFVCLHVDKGLNMTETKLPLQGKYSLVYCGTLKS
                     GNIFAVFAPNEGMSDTKKYYYIECSADSKVAKSSEFTSPSPNMLIMDYSEANGDLYFI
                     AGSDKSTEAYNKVFTSYAPIDNPGYSTSNNKQMDKYEKRAMGTEFANLHLLRFSDGKL
                     AFASTEPVKDFKTKMVTPPSQKKGQPYTGKKIVIENLFVAPSGDVLVAGQLSDKKVVN
                     GGNSYEFRYYDYVCVQFDNQGKLVAQYAVNKMLDDSKNEVLSAIQNFVPSADGKSVYW
                     EILEVQAAKYYASVAAAYSGNYSLSSHYFPRIANITLSNHSVSNFTELGEKGKYLVYH
                     SITSLTDKDNNRFYIGHDEDYKNIWLGKYSFR"
     gene            complement(2806..3429)
                     /locus_tag="PK134_03210"
     CDS             complement(2806..3429)
                     /locus_tag="PK134_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB37887.1"
                     /translation="MTIQVGIIEDEKHIRENLQILIDGSEGFSCKHLFANAEDAINII
                     PTLQLDVVLTDIQLGGKTGIDCILELKDKCPSTNFLICTSFDDSEHVFKALKTGAMGY
                     LTKTTQPSKLLDSIVDVYRGGSPMNSHIARKVVSSFMETPVSPNYEKLSERERLIVDL
                     LSKGMRYKEIADQLFLSTETVRTHIRNIYHKLQVNSRTEALNKVFKK"
     gene            complement(3483..5504)
                     /locus_tag="PK134_03215"
     CDS             complement(3483..5504)
                     /locus_tag="PK134_03215"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRB37888.1"
                     /translation="MSRFLSRIIFCVCLFFSHCVLYASDTTPTAQQKQIDTRISEAEE
                     IYKRDFKHAYVITLEAKSHAVKINYLQGIVASSCLQSIIESQTNKSIPAIAALTNLLQ
                     SSNGINLKLKQQLLSTLGICYFYIGKLDSAQFYFTESISSYSAHDTSIQLFNSCLQLS
                     EVFIKEGIPSKALDLYGKAYKIYQQNTSKEGEAWLNLVLGKIYYRQNLYEQSISTINT
                     TISQFASLNSLTGQISSLIQNGNSYYKQMLDDSAANCYQQALMYATTLGDERNKSICY
                     SNLSRIYLEHNKTKQALETALHALEVIHPGNYPEIEAGTYQQLGDIYVELGQKSKAIE
                     WIQKALLLAKDNNNKTIERDCYKSLSELYASIKQYEPAMENLLQAYRIKDSIQPIAFN
                     NRLAEMEARYSLEKKESTIKLLNQQSLINELRIKEQKENIRKQNALVIILFLLIATII
                     IALYFYLRWRKQAELINQQNTIRQTEENERMRIAKDIHDELGSGLTKIKFLSEILEMK
                     TKDQEQLKETLQTITGTSHQLIDNMRDMIWNMNPMNSTLDNLIARVREFSTEYLEDFP
                     IEVKYDLPENIPSEKISIEISRNILMIVKESLQNIVKHSNASKVSIRIEIQPVFKLII
                     TENGIGFNLNEKTTGNGLRNITERSKAIGASLKISSKPGEGSILSLEKI"
     gene            complement(5599..8253)
                     /gene="cphA"
                     /locus_tag="PK134_03220"
     CDS             complement(5599..8253)
                     /gene="cphA"
                     /locus_tag="PK134_03220"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRB37889.1"
                     /translation="MRILEIRPMRGPNFWSIRRHKLIVMKLDLQEMEERPTDKIDGFL
                     ERLKSMFPSMYSHRCSEDHEGGFFKRVEEGTWMGHVIEHIALELQTLAGMDTGFGRTR
                     GTGEHGVYHVVFSYIEEKAGVYAARASVRIAQALIDGTGYDLQNDIQQLREIREDERL
                     GPSTGSIVEEAASRGIPWIRLNRHSLVQLGYGINQRRIQATIASTTSSIAVEIACDKE
                     DTKNLLEAQSIPVPRGKIIRDEDGLKDAVEDLGYPLVIKPLNGNHGRGATINIVNWDQ
                     AVEALEIAKKISRSVIVEKYITGYDHRILVINYKFICAAQRKPAAVVGDGKSTIQQLV
                     DAVNADPRRGYGHENVLTSIKIDEMTQNILEEKELTLQSVLKKGEELFLKRTANLSTG
                     GTATDVTDIVHPYNVFMCERIARIIGLDICGIDIMTPDISEPLTETGGAVLEVNAAPG
                     FRMHIAPTDGLPRNVAEPVVDMLYPPGSNYRIPIIAVTGTNGKTTTTRLIAHIAKTCG
                     HKVGFTTTDGIYIQNQMLQRGDCTGPQSAEFVLKDPTVDFAVLETARGGILRAGLGFH
                     RCDIAVVTNVAADHLGLKGINTLEEMSKVKAVVPESVQPNGYAILNADDELVSNMARN
                     LDCKVAYFSLDENNPLIKRHCEDGGLAAILENGFVTICKGTWKLRVHKVINIPLTFSG
                     KAVFMIQNILPAILSAFIRNFKIEDIRLALETFIPSPVQTPGRMNMFQFKKFNVMVDY
                     AHNPAGFQAIARFLEKVDAKPKVGVIAGVGDRRDEDIIALGTLAAKMFDDIIIRQDRN
                     LRGRTEQEIIDFMLDGIKAVDPTKPVKVIPLEKEAIDYAIKNGKKGSFIVICSDVIPD
                     ALDQIMKFKEKEDQFEMHPDDIPNQSNR"
BASE COUNT         2447 a         1605 c         1564 g         2945 t
ORIGIN      
        1 tggctggtgg ccgggtttgt atgaaatgaa tggccttgat ttttattttg gttttaaata
       61 caccagtgac atttacgata acatccgatt taaaaaaggt aaaggtggtc attgtcctgc
      121 ttacaagtga aagatgatga tcagccacgg ctgacagggg tgaaaggtga tgaagagaag
      181 agtgatgaag taaattagat gattagataa tctgataatt tgataattca gagtatttgt
      241 gtgtaccgct tacattttct taccgtttat attatcagat agaatttaaa aagataaaag
      301 gagacagcag aacgttttct gctgaactcc tttaagggtg ggaataaaat ccccgttttt
      361 tatagggtac gttgaccatc aacggtaacg gtattgccgc tgttgtctgt aaatgtgcca
      421 tcaatctcat ctccggaaag tgttccaacc tttatattgt tatcatcata taaatcaccg
      481 gatgatgtaa tagttccgga aacatcattg gttgatgtct gaccattttc ccgtgatatt
      541 ccgccccatt gacctaaagg tcgcgagagt agaatattga acgttcctga ctcaggttct
      601 gtagtagaat aggtgccttc aaaaccctca accaatgcat ttgaagtttc tttgataatg
      661 gtaaatactg catttggatg acccggaatg tttgatgaaa ttatttgtgg agtacttccg
      721 ttgttctgaa cagagaaagt gatgcttaca gcctgaccat ttaatgttcc tgtaaaaggt
      781 gcaatgtaag gctgaccact aacccaactg actgtagagg ttagcgtagc agaaacgcca
      841 tcaacaacca ttgttgcagt aatatcatta gtgccattct gaatggaaaa ctttatggtg
      901 cctgatgatc caatgatgat gcctttgtaa acacctttgc tgcttccatc gtttgcagct
      961 aatgcttctg cctgagttac acaactgctg catgtgtaat gagggttgtc tttgtctttc
     1021 tttttgcaag aagtgaatgc caatatggca atgcataaaa tgggaatgat taactttttc
     1081 atttatttaa tttttatttt gtgatttata aattattacc ggaaagagta tttgcctaac
     1141 caaatgtttt tataatcttc atcatgtcct atgtagaagc ggttgttgtc tttgtctgtc
     1201 agactagtga tactatgata aactaaatat tttccttttt caccaagctc ggtaaagttg
     1261 cttacagaat gattagacaa ggtaatattg gcaatgcgtg gaaagtaatg cgatgaaaga
     1321 gagtaattgc cactatatgc tgctgctact gatgcatagt atttagcagc ttgaacttct
     1381 aaaatttccc aatagacaga tttgccatca gcagacggaa cgaagttttg aattgctgaa
     1441 agaacttcat ttttactatc atctaacatt ttgtttacag catactgtgc tactagtttt
     1501 ccctgattgt caaattgcac acagacatag tcgtaatatc tgaattcata ggagttgccg
     1561 ccattcacta cttttttatc agacaactga cctgcaacaa gcacatcgcc tgaaggtgca
     1621 acaaaaagat tttcaataac tatcttctta ccggtataag gttgaccttt tttctgtgaa
     1681 ggaggcgtaa ccatttttgt tttaaaatct tttaccggtt cagtcgaagc aaatgcaagt
     1741 ttgccatctg agaatctcaa taaatgcaaa ttagcaaatt cagttcccat agcacgtttt
     1801 tcgtatttgt ccatttgctt gttgttggaa gtagaatatc cgggattgtc aattggtgca
     1861 tagctggtaa aaactttatt ataagcctca gtgcttttgt cagatccggc aatgaaatac
     1921 aagtctccat tagcttcgct ataatccatt atcaacatgt tgggcgatgg cgaagtaaac
     1981 tcactgcttt ttgcaacttt actgtctgca ctacattcaa tgtaataata tttcttggtg
     2041 tcgctcatgc cttcattagg tgcaaataca gcgaaaatat ttcctgattt cagtgtacca
     2101 caataaacta aggagtactt accttgtaat ggtagttttg tttcagtcat gtttaaacct
     2161 ttgtcaacgt gaaggcaaac gaactgatca tcttcaccac tatcataact tgcaagaata
     2221 agcacactac cgtcatcgtc attaccataa gccgcaaagc ctctgtactt gctatcgtta
     2281 ttctttggtt ttaccacttc tttagaaatg cggctgcccc attgatattt ttgtttatcg
     2341 aagttccagt ttctttccca gacttcctgt tgcagattca gtgacatact cataacatta
     2401 aatgaattgg taccaccaac aaaagctttt atcaaagtaa ctttttggtc gggttgtgtt
     2461 tgtttgtctt ctttagtttt ttctactcct ataaatttca gatttttatc aaagacataa
     2521 tcttcataat taacctgatc actctttttg tcaaccttca ttttgtaagt cattctgatg
     2581 ttgccgtcag cacttagttc ggcatttgcc agaatgcctt tttgggcagg ctttgacaat
     2641 tcgtgagttg tttcattgcc ttgtgcaatg agtagggtcg gggagaggat ggcagaagca
     2701 agccatactg cggtaacttt tggtagcatt tgtttcattt tgcgtttttt tatgaaacaa
     2761 aattagcccc tgtattatgg gtagtcaatc accatttagt agtaattatt ttttgaatac
     2821 tttgttaagt gcttcagttc ttgagtttac ctgaagtttg tgataaatgt tcctaatgtg
     2881 tgtgcgaaca gtttcagtac ttaaaaaaag ttggtcggca atttctttat atcgcatgcc
     2941 tttagaaagt aaatcaacaa tgagtcgctc acgctctgat aatttttcgt agttggggct
     3001 tacaggtgtt tccataaaac ttgaaactac tttacgtgca atatgactgt tcattggtga
     3061 accacctctg taaacatcaa ctattgaatc taataatttt gatggctgtg ttgttttagt
     3121 gagatagccc atggcaccgg ttttcagagc tttaaaaaca tgctcagaat catcgaatga
     3181 agtacatatg agaaaattgg ttgacgggca tttgtctttt aattcaagaa tacaatctat
     3241 gcctgtcttg cctcctaact gaatgtctgt cagcactaca tcaagttgca gcgtaggtat
     3301 gatatttata gcatcttcag catttgcaaa taaatgtttg caggagaaac cttcactgcc
     3361 atcaattaat atctgcagat tctctctgat gtgcttttcg tcttcaatga tacccacctg
     3421 aattgtcata aaacaaaaat aaacctttca ttagtgaatg caatactact aaagtagtat
     3481 attcagattt tttccaaaga aagtattgat ccttctcccg gtttagaaga tatttttaat
     3541 gatgctccaa tagctttact tctctcagtt atatttctca gcccgtttcc tgttgtcttt
     3601 tcattcaagt taaagcctat tccattttca gtaattatta gtttaaaaac gggttgaatt
     3661 tctatcctga ttgaaacctt agaggcattt gaatgcttta cgatgttttg taaggattct
     3721 ttaactatca tcagaatatt tctgctgatt tcaatactta ttttttctga aggtatattt
     3781 tcaggcaagt catattttac ttctatcgga aaatcttcca agtactctgt tgagaattca
     3841 cgtacacgtg caatcagatt gtctaatgtt gaattcatcg gattcatatt ccagatcata
     3901 tcacgcatgt tgtcaatcaa ttgatgcgaa gtgcctgtaa ttgtctgtaa agtttctttt
     3961 aactgctcct ggtctttggt tttcatttct aaaatttctg acaggaattt aatttttgtt
     4021 aatccggaac ccaattcatc atgaatgtcc tttgcaattc gcattcgttc attttcttct
     4081 gtttgtctga tagtgttttg ttgatttatc aactcagctt gtttgcgcca tcttagatag
     4141 aaatagaggg caatgatgat tgttgcaatg agtaaaaaca gaataataac taaagcattt
     4201 tgttttctga tattttcctt ttgctctttt attcgaagtt cattaattaa tgattgctga
     4261 ttgagaagtt tgatagttga ctctttcttt tcaaggctgt aacgcgcttc catttctgcc
     4321 aacctgttat taaatgcaat tggttgtatg ctgtctttga ttctgtaagc ctgaagtaga
     4381 ttttccattg caggttcata ctgctttatt gatgcatata gttcagaaag tgatttatag
     4441 caatcgcgtt caatagtttt gttattattg tcttttgcca gcaataatgc tttctgaatc
     4501 cattcaatgg ctttactttt ttgaccaagt tccacataga tatcaccaag ttgctgatag
     4561 gtgcctgcct ctatctcagg gtagtttccg ggatgaatga cctcaagagc gtgtagtgct
     4621 gtttcaagtg cttgtttggt cttgttatgt tccagataaa tgcgactaag gttagagtag
     4681 caaatagatt tgttgcgttc atcacctaag gttgttgcat acattagcgc ttgctgatag
     4741 caatttgcag cgctgtcatc gagcatctgt ttgtagtagg agttgccatt ctgaataagt
     4801 gaagatattt gtccggtcag actattcaaa ctggcaaatt gactgatagt tgtgtttatg
     4861 gtactaatag attgttcata aagattttgc ctgtaataaa ttttacctaa aactaaattc
     4921 agccaagctt ccccttcctt gctcgtattt tgctgatata ttttataagc tttgccataa
     4981 aggtcaagag cttttgaagg aataccttct ttgatgaaaa cttccgatag ttgaagacag
     5041 gaattaaaga gttgaataga agtgtcatgt gccgaatatg aacttatgct ttcagtaaaa
     5101 taaaattgag cagaatcaag tttgccaata taaaaataac aaatacctaa agttgatagt
     5161 agctgttgtt ttaattttaa attaatacca tttgatgatt gcagtaaatt agttaaagca
     5221 gcaattgcag gaatactttt atttgtttgt gattcaatta tactctgcaa gcatgagcta
     5281 gcaacaattc cttgcagata attgatttta actgcatgag attttgcttc taatgtaatt
     5341 acataagcat gcttaaagtc tcgtttgtaa atttcttcag cttctgatat tcttgtatca
     5401 atctgttttt gttgtgctgt aggagtagtg tctgatgcat ataatacaca gtgggaaaaa
     5461 aacaaacaaa cacaaaatat tattcttgac agaaaacgcg acattaaatt ggaaatcgca
     5521 tgctaagata ataaaacaat attactataa gcgagtaatc atgtcagcaa agaactttaa
     5581 attcttaaaa aatataattt acctgtttga ctgattagga atatcgtcag gatgcatttc
     5641 aaattgatct tctttttctt taaacttcat tatttgatca agtgcatctg gaataacatc
     5701 gctacatata acaatgaatg aacctttttt gccattctta attgcataat caatagcttc
     5761 cttttctaat ggaattactt taacaggctt tgttgggtca acagctttta ttccatcaag
     5821 cataaagtca attatttcct gttctgttct gcctcgtaga tttctgtctt gtctgatgat
     5881 aatatcatca aacattttag ctgcaagcgt tcctaatgca atgatgtctt catcgcgtct
     5941 gtcgccaaca cctgcaatga cacctacttt tggtttagca tcaacttttt cgaggaaacg
     6001 tgcaatagct tggaagcctg cagggttatg tgcatagtca accatgacat tgaatttctt
     6061 gaactgaaac atattcatcc tgcctggagt ttgaaccgga gaagggataa atgtttccaa
     6121 tgcaagacga atgtcttcaa ttttaaaatt gcggataaag gctgaaagaa ttgcaggtaa
     6181 tatattctga atcataaaca ctgctttgcc tgaaaaggtg agtggaatgt taatgacttt
     6241 atgtacgcga agtttccatg tgcctttgca aatagttaca aatccatttt caagaatggc
     6301 agcaagacct ccatcttcgc aatgacgttt tatcaacgga ttgttttcat cgaggctgaa
     6361 ataagccact ttgcaatcga gatttcgagc catgttagac actaattcat catcagcatt
     6421 taaaattgca taaccattgg gttgtacact ttcaggaacc actgctttaa ctttcgacat
     6481 ttcttcaagt gtgttgatgc ctttaagtcc aagatgatct gctgcaacgt tggttacaac
     6541 tgcaatatca caacgatgaa atccaagacc ggcacgcagt attccgcctc ttgcagtttc
     6601 taataccgca aaatcaacag taggatcttt caatacaaac tctgcacttt gtggacctgt
     6661 acaatcgcca cgctgcaaca tctgattttg aatatatatt ccatcagtag tggtaaatcc
     6721 aactttatgc ccacaggttt ttgcaatgtg agcaatcaaa cgtgttgttg ttgttttacc
     6781 atttgtacct gtgacagcaa taattggaat gcgataatta cttcccggag gataaagcat
     6841 atcaacaaca ggctctgcaa cattacgtgg cagtccatct gtaggcgcaa tgtgcattct
     6901 gaatccggga gcagcattaa cttccagtac ggcaccaccg gtttctgtca gtggttcact
     6961 gatgtcggga gtcataatgt caataccgca aatgtcgagt ccaataattc ttgcaatacg
     7021 ttcgcacata aaaacattgt atggatgcac aatgtcggta acatcggttg ctgttccacc
     7081 tgtactcagg tttgcagtac gtttcagaaa caactcttct ccttttttaa gaacgctttg
     7141 aagggtcagt tctttttctt ctaaaatatt ctgtgtcatc tcatcaattt ttattgatgt
     7201 cagcacattt tcatgtccat aacctctgcg tggatcagca tttacggcat caaccaactg
     7261 ttgtattgtg cttttgccat cgccaacaac tgcagcaggt ttgcgttgtg ctgcacaaat
     7321 aaatttgtag ttgatgacta aaatccgatg atcataacct gtaatatact tttctacaat
     7381 tacagaacgt gaaattttct tggctatttc taatgcctct actgcctgat cccaattgac
     7441 aatatttatt gttgctccac gaccatgatt accattcaat ggttttataa ctaaaggata
     7501 gcccaaatct tcaacagcat ctttcaagcc atcttcatca cgaattattt ttcctcttgg
     7561 tacaggaata gattgtgctt cgagcaggtt tttagtgtct tctttatcac aggcaatctc
     7621 tacagctatg ctgctggtag tgcttgcaat agtagcctgt attctccgtt gatttattcc
     7681 ataccctaat tgcactaagg agtgcctgtt caacctaatc catggaattc ctcttgaagc
     7741 tgcttcttca acaatggagc ctgtgcttgg gccaagacgt tcatcttcac gtatctcacg
     7801 caattgctga atgtcatttt gcaaatcata gcctgtgcca tcaattaatg cctgtgcaat
     7861 acgcacagag gcacgtgctg catacacacc tgctttttct tcaatgtagg agaacacaac
     7921 gtgatataca ccatgttcgc ctgtgccgcg cgtacggcca aagcctgtgt ccatgcctgc
     7981 aagtgtttgt agttcgaggg caatgtgctc aataacatgt cccatccagg tgccttcttc
     8041 aacacgttta aaaaatcctc cttcatggtc ttcgctgcat cggtgcgaat acatacttgg
     8101 aaacatgctt tttaatcttt ctaaaaagcc atcaattttg tcagtaggtc gctcttccat
     8161 ctcctgaagg tcgagtttca ttacaataag tttatgtctg cgtatgctcc agaagttggg
     8221 ccctctcatt gggcgaattt caagtatcct cataaaaaaa accttttggt tgtggcacga
     8281 atatagagac ttgatttgaa tattgaaaaa cttggtgaaa ctgcgaagag aaaaatagct
     8341 taattcaata cagaatagat ttagtgaatc gaagtattat tcaatttttg attcagatat
     8401 gcatctaaga aaaaagaggt tatctcaata tttgaaatag cctcttttta aggattattt
     8461 tacgctaatt aaggacaaac aacttctatt tgccatgcag taccactatt acaaccgcta
     8521 acataaattt caactaatct gctttttcca gggtcataat t
//
LOCUS       DAORQT010000053         8403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1198_length_8403_cov_9.808816, whole genome shotgun sequence.
ACCESSION   DAORQT010000053 DAORQT010000000
VERSION     DAORQT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1198_length_8403_cov_9.808816"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PK134_03225"
     CDS             <1..876
                     /locus_tag="PK134_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37890.1"
                     /translation="NQQKELIDKIKSAMKMARSERIHPVCDKKLITSWNAMMISALAD
                     AALYFNIKEYELQAITTFNYLEQNLFIEEGRLLHSSGTKQNSNLHLEDYALLTQACIK
                     LFECKWDFQWIEKAISLTGYAIQYFSHDDNVLFYDYTANDSVLQFRKTELQDNVIPCA
                     NSVMANNLFLLSKYLNNENYLLRSKKMLEKVIDQIPNYGFGYANWALLLQRINQPFYE
                     VAFGGYNALKNKNKLNEYFLPMKITAPIILKDKIEFTADKNETDDLIYVCRNHSCLLP
                     TSDIIKVAQTISDQQ"
     gene            873..1313
                     /locus_tag="PK134_03230"
     CDS             873..1313
                     /locus_tag="PK134_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37891.1"
                     /translation="MKQNVFLLCVILNITITGCQIFKINKSDKHGNRQGHWEHYWNDE
                     GTIQRKGKYKHGLEVKKWKYYDLNGKLIKQEKYSKKRDVINIKTYYDNKKIKSNGKAF
                     LINENDSILHFYWQGPWNYYSPTGVLEKTEWYEKGKIKKAVTYP"
     gene            complement(1339..1833)
                     /locus_tag="PK134_03235"
     CDS             complement(1339..1833)
                     /locus_tag="PK134_03235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB37892.1"
                     /translation="MAEETLNMTATTFENLSQPNAAMDVFPINYSEKHIAELIHEIRR
                     RVFVLEQHIDPMIEFDTFEHESQHYLAYVKNIPAGTARWRHTDEGIKLERFSVLKEHR
                     NSGLGRMLVNRLLNDVKPIGKKVYLHAQTTTVRFYEKAGFKVVSELFYEANIPHFKME
                     LVIS"
     gene            complement(1845..>4662)
                     /locus_tag="PK134_03240"
     CDS             complement(1845..>4662)
                     /locus_tag="PK134_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37893.1"
                     /translation="SYTASSLFVFSNNFTNNALQRIDVLKDFSVSNGNVGLAFTSGKL
                     NTHISGNLNVSGSSTQLVSQFYPYGTDSVKFVIDGNCSLNGGMFNVSWNRGPVSFISS
                     GIFNQVNSNFGVVFHPFPGNYAACSVFIDSLNFSGGTFKVFDSYITDGKTVSITIGSD
                     VNINFQNSSDRFVLISNTGWWNNPELNLVINGDLKISGNSSAIFASNVGRGEENIVIN
                     GDFEIQGGNNAIGGSTSLGSQQHDISVLINGNMTQTGGQFYYSVNEGNVDYNIQNNLI
                     QTGGTQNLTYQDAAAKLNIGGQYLQSGGTFNFHASTGNTSLSDSVFVQGNFSQSNGVL
                     RFDNAQGSSNISQNVLILNSDSILLSGNGIITHANHLTTNTVFGKILFNKNGISTFQR
                     ATSTHDLQQVEMVVGSLTTLDVKSSIQPLLIASHVSSAPAVNNVFTINGVLDMGLQRI
                     MARQQGNYYSQLSVGATGKIKTMNPNGFYSTSAASTINTLIAGNNRMNFWLDAQSIIE
                     YNGTQNQNITGIPNGIVNAVSNKYGKLQINFAGVNNINFVKPEADSVVFVRSGLMLTS
                     GELNLNADHIANTNGLSIVLENGCQILHASGYIRSETIDGSARIHLSSQQSVNYEIPF
                     GFNSTTYIPFKYQCSSGCDWVSLATYRSLPDNTPFPPGVTHVRSISGADNSLQTIDRY
                     WYVRAVGNPNANLRFTYAPIEKGVISTPRAQRWIGNDAWESFKGSQSNPSPFETDVNG
                     ITGLNSWWTLSSQSSPLPVEILSFGANCQSQSVILKWSVATQLNNDIFILERSRDGKK
                     WENLTSIKGAGTTNTLMNYETKDEAPYSEVTYYRLAQIDYDGKRNELKTIAVKGCGSA
                     PIRILYYSFSDHKTVIKIQSSYNDKIEIFLFNENGQTVARKVQNISNNIQEIEMDCPQ
                     LNTGIYMLSIRGSFNNIIKKLVKN"
     assembly_gap    4663..4684
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4685..6158)
                     /locus_tag="PK134_03245"
     CDS             complement(<4685..6158)
                     /locus_tag="PK134_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37894.1"
                     /translation="MSRISYLILMLFFVNVSVNAAVTVSPATSGNCLNVTPSAFLPIG
                     NIVITEGANGDFAIQSNKTLILSAPTGFMFQPGSGNVSFLASRNITTASIAVTLTTIT
                     VTISVSGIPKADELIISNIYARASTSNVSGNILRKSPGGGTATIAGDVAGAGINHGTL
                     SSAGTGQIVTSVANGAWSNPATWQGGKIPLCTDNVIIYHQINVDVVTPSLASLIIKSG
                     AKLTANLPVTVNSNLTIEANAWYVHNNSSNAATTIFNGTEAIDDNATIEVNNWSAATV
                     PFMSGVSSTVGNLILNYNAVWQQDGLFAPSKIKGNVSVTNGQVVMDDGTGMSTLLNLN
                     HVSIYKTGAIIFAQGANRNLTLNTLSFTDSSKSNAITALMFRTYGTLLWNNAGSVYLS
                     HDFSAVEGTTGYNAFNATVSIGGDLNVVKGKVDFFRYVDGNGVIQVAGNTYCNLTAGN
                     WFRLADYNTINLDFTTNNFSISGTESCYLQGSDGSTNFNIN"
     gene            complement(6245..7168)
                     /locus_tag="PK134_03250"
     CDS             complement(6245..7168)
                     /locus_tag="PK134_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRB37895.1"
                     /translation="MPVNHQEVQSLSHLELLAKQVVEGFITGLHKSPFHGFSVEFAEH
                     RLYNTGESTRHIDWKLFGRTDKLFVKRFEEETNLRCHIVIDNSASMHYPEVKGGKSLQ
                     NKITFSVYSAAAILYMMKQQRDAIGLTVFSEQIDVQTQAKSSTAHHKLVFAHLEKLLE
                     NKTDVNRKTATADALHQLAESIHKRSLIIIFSDMFENIDSNEALFKSLQHLKHNKHEV
                     ILFHVTDKKHELDFQFENRPYRFVDVETGEEIKVHSNLVREKYIEAMKEYKSMLVHKC
                     AQYKIDFVQADIHEGYHQVLLAYLLKREKLH"
     gene            complement(7192..7512)
                     /gene="trxA"
                     /locus_tag="PK134_03255"
     CDS             complement(7192..7512)
                     /gene="trxA"
                     /locus_tag="PK134_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRB37896.1"
                     /translation="MAIEITDSNFEEVVMKSDKPVLVDFWAEWCGPCRMVGPIVEELA
                     KDYEGKAVIGKIDVDSNPNVTMQFGVRNIPTILFFKNGQVVDKQVGAAPKNILAKKID
                     TQLV"
     gene            complement(7670..>8403)
                     /locus_tag="PK134_03260"
     CDS             complement(7670..>8403)
                     /locus_tag="PK134_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRB37897.1"
                     /translation="KSSTQHTLFGDLGGVDASKPKIIPTEHFSNLEQLKKEKEIIGIY
                     LSGHPLDDYKEEIKTFCNISVEQLNELKLLNNRDVNFAGIITNANHRVSKTGKPFGSF
                     TIEDYNASFEFAVFSEEYAKFKHLLNNDAFVFIKARVQTKFYNEKEFELKINSVHFLD
                     EVANKMAKGIMISFRSESINEMNIKKLKEISKNSKGKLTVKFSIRDNDQNLSIDVESN
                     QLKLEYNKELFKELNSISERVSLIK"
BASE COUNT         2664 a         1568 c         1346 g         2803 t
ORIGIN      
        1 aatcaacaga aagaactcat tgataaaata aaatcagcca tgaaaatggc aagaagtgaa
       61 cgcattcatc cggtttgcga taaaaaactt atcacttcat ggaatgccat gatgatatcg
      121 gcattagcag atgcagcatt atattttaat attaaagaat atgaattgca ggcaataacc
      181 accttcaact atttggaaca aaacctgttt atagaagaag gacgactact tcatagctcc
      241 ggaacaaaac aaaacagcaa tcttcattta gaggattatg cacttctcac tcaagcatgc
      301 attaaactat ttgagtgcaa atgggacttt cagtggattg agaaagcaat ttctttaacg
      361 ggctacgcta ttcaatattt tagtcacgat gataatgttt tattttatga ctatacagca
      421 aacgattctg ttctgcaatt cagaaaaact gagcttcagg ataatgttat tccctgtgca
      481 aattcagtaa tggcgaacaa tttattctta ctttcaaaat acctcaacaa tgaaaattat
      541 ttgttgcgat caaaaaaaat gcttgaaaaa gtaattgatc aaattccaaa ttatggattt
      601 ggttatgcaa actgggcctt actattacaa agaataaatc aaccattcta tgaagtagct
      661 tttggtggtt acaatgcttt aaaaaataaa aataaactta atgagtattt tctaccaatg
      721 aaaatcactg caccaataat tctaaaagat aaaattgaat ttactgctga taaaaatgaa
      781 actgatgact tgatttatgt atgcagaaac cactcgtgtt tacttccaac atcagatatt
      841 attaaagttg cgcaaactat ttctgaccaa caatgaagca aaacgtattc ttactttgtg
      901 taattttaaa tattacaata acggggtgtc agatttttaa aattaacaaa tcagacaaac
      961 atggaaaccg gcaaggacat tgggagcatt attggaatga tgaggggacc attcaacgaa
     1021 aggggaaata caaacacggc ttagaggtaa aaaaatggaa atattacgat ttaaatggca
     1081 aactgataaa acaagaaaaa tacagtaaga aaagagatgt cattaatata aaaacatatt
     1141 atgacaacaa aaaaataaag tctaatggta aggcattttt aatcaatgaa aacgatagca
     1201 tactacattt ttactggcag ggaccatgga actactactc acctacaggt gtgttagaaa
     1261 aaacagaatg gtatgaaaaa gggaaaataa aaaaggctgt cacgtaccca taacagcctt
     1321 taaaactcta aatatatgtc atgaaattac aagctccatt ttgaaatgcg gaatattagc
     1381 ttcatagaat agctcgctaa caactttaaa tcctgctttt tcatagaatc taacagtggt
     1441 agtttgtgca tgcaagtaaa ctttcttacc tattggcttt acatcgttta acaacctgtt
     1501 aaccaacata cggcctaaac ctgaatttct gtgctctttt agaacgctga atctttcaag
     1561 cttaattccc tcatcagtat gacgccatct ggcagtacct gcaggaatat ttttaacgta
     1621 agccaaataa tgttggcttt catgctcaaa tgtatcgaac tcaatcattg ggtctatatg
     1681 ttgttcaaga acgaacaccc ttcgtctgat ctcatggatg agttctgcaa tatgcttctc
     1741 ggaataatta atcggaaaca catccattgc ggcattgggc tgtgagagat tttcaaatgt
     1801 agttgcggtc atatttaaag tttcttcggc cataaaaata gaaattaatt cttaactaat
     1861 tttttgatta tattattaaa ggaacccctg atgcttagca tgtaaattcc tgtattaagt
     1921 tgtggacaat ccatttcaat ttcttgtatg ttgttgctta tgttttgcac ttttcgtgcc
     1981 actgtttgtc cgttttcatt aaatagaaaa atctctattt tatcattgta agaagattgt
     2041 atttttataa ctgttttatg gtcactgaaa gaatagtata atattcttat cggggcggaa
     2101 ccacagcctt ttactgctat tgtcttcagc tcatttctct ttccatcata gtcaatttga
     2161 gctaacctgt aataggtaac ctccgaatat ggtgcttcat ccttagtttc ataattcatt
     2221 aatgtattgg tcgtacctgc acctttaatg gatgtaaggt tttcccattt ttttccatct
     2281 ctgcttcgct ctaaaataaa gatatcatta ttgagctgtg tggcaacaga ccattttaat
     2341 atcacactct gactctggca attggcgcca aaacttaata tttctacagg caaaggactt
     2401 gactgactgc ttagtgtcca ccatgaattt aatccggtaa ttccgttaac gtctgtttcg
     2461 aatggtgatg gattggattg cgaaccttta aatgattccc atgcatcgtt acctatccat
     2521 cgctgagcac gaggtgttga tattacccct ttttcaatag gtgcataagt gaatctcaaa
     2581 ttagcattag gatttccaac cgctctaaca taccaatagc gatcaattgt ttgtaaacta
     2641 ttgtcagcac cggaaattga acggacatgt gttacccccg gaggaaatgg tgtattatca
     2701 ggcaaactcc tgtatgttgc caatgaaacc caatcacatc cacttgaaca ttgatatttg
     2761 aatggtatat aagttgtgct gttaaaacca aatggaattt cataattaac actctgttgt
     2821 gatgaaagat gaattcttgc actaccgtca atagtttcac ttctgatata acctgaagca
     2881 tgtaaaatct gacagccatt ttctaacaca attgaaagtc catttgtatt tgcgatatga
     2941 tccgcattaa gattcaattc accggaagta agcattaatc cagagcgaac aaaaaccaca
     3001 ctgtcggctt cgggctttac gaagttaata ttgtttactc cggcaaagtt aatttgtagt
     3061 ttgccatatt tatttgaaac tgcattgact ataccatttg gaatacctgt gatgttctga
     3121 ttttgggttc cattatattc aataatgctt tgagcatcga gccaaaaatt cattctgtta
     3181 ttacctgcaa tgagtgtatt tattgttgat gccgcagaag ttgaataaaa accatttgga
     3241 ttcattgttt taattttacc ggttgcacca actgaaagtt gcgaatagta atttccttgt
     3301 tgacgtgcca taattctctg taatcccata tcaagcacac cattaattgt aaacacatta
     3361 tttactgccg gtgctgatga aacatgtgaa gctatcagca atggttgaat ggatgatttc
     3421 acatcaagtg ttgttaaaga ccctacaacc atttctacct gttgtaaatc gtgtgttgaa
     3481 gttgctcgtt gaaatgtgga aattccattc ttattaaaca aaatttttcc aaataccgtg
     3541 tttgttgtaa gatgattggc atgagtaata attccatttc ctgaaagtaa aatactatca
     3601 gagtttaaga ttaatacatt ttgacttata tttgatgacc cttgtgcatt atcgaagcgc
     3661 aaaactccgt tgctctgact aaaatttcct tgtacaaata cactatcaga aagtgatgta
     3721 ttaccggtgc ttgcgtgaaa attaaaagta ccaccagatt gtaaatattg tcctccaata
     3781 ttaagctttg cagccgcatc ctgataagtc agattctgtg tgcctccagt ttgaattaaa
     3841 ttattttgaa tattgtaatc aacattacct tcattaacag aataataaaa ctggccgccc
     3901 gtctgagtca tgtttccatt aattaatact gatatatcat gctgctgtga tcctaaagaa
     3961 gttgagccgc caattgcatt attacctccc tgaatttcaa aatcaccatt tattacaata
     4021 ttttcttctc cacgtccaac attactcgca aaaattgcac tgctgtttcc actgattttt
     4081 aaatcaccat taataactaa attgagttca ggattattcc accagcctgt attgctaata
     4141 agaacaaatc tgtcacttga attttgaaaa ttaatattta cgtctgatcc tattgttata
     4201 gaaactgttt ttccatcggt gatataactg tcaaaaactt tgaatgttcc tccggaaaaa
     4261 ttaaggctgt caataaaaac agaacatgct gcataattac cagggaatgg atgaaaaacg
     4321 actccaaaat ttgaattcac ttgattaaat attccgcttg aaataaaact aaccgggcca
     4381 cgattccatg aaacattaaa catgcctccg tttaagctac aattaccatc tatcacaaat
     4441 tttactgagt ccgttccata gggataaaac tgcgacacta attgtgttga cgacccactt
     4501 acatttaaat ttcctgaaat atgagtattt aactttccac ttgtgaaagc caatccgaca
     4561 tttccatttg aaacagaaaa atcttttagc acatcaattc gttgtaatgc attattggta
     4621 aaattattac tgaaaacaaa taagctgctt gctgtataac tannnnnnnn nnnnnnnnnn
     4681 nnnncattaa tattaaaatt tgttgagcca tcagaaccct gaagataaca actctctgtt
     4741 ccgcttattg aaaaattatt ggtagtaaaa tcaagattta ttgtattata atctgctagt
     4801 cgaaaccagt taccggctgt cagattacaa taagtattcc ctgcaacctg aataacgcca
     4861 ttaccatcaa catatctgaa gaaatcaact ttccctttta caacattcaa atctcctcca
     4921 atactaacag tagcattaaa tgcattatag cctgttgtac cttcaactgc actaaaatca
     4981 tggcttagat aaacactacc tgcattattc cacaacaaag taccatacgt tctaaacatc
     5041 aatgctgtta ttgcattgct tttactgcta tcggtaaacg acagtgtatt taatgtcagg
     5101 tttctattag caccttgtgc gaaaataatt gcacctgttt tatatatgct tacatgatta
     5161 agatttaaaa gtgttgacat acctgtacca tcatccatca caacctgacc attcgttact
     5221 gatacatttc cctttatttt tgatggagca aacagtccat cctgttgcca tactgcatta
     5281 tagtttaata taagattacc gactgtcgaa ctgacaccgg acataaaagg cacagtggct
     5341 gcactccagt tattaacttc tatggtagca ttgtcatcaa tagcttctgt tccattaaat
     5401 attgttgttg ctgcatttga actattgttg tgtacgtacc aagcatttgc ctcaatggtg
     5461 agattactgt taactgttac aggtaaatta gcagtgagtt ttgctcctga ttttattatt
     5521 agtgaagcta aggaaggtgt cactacatct acattaatct gatgatagat tattacatta
     5581 tcagtacata acggtatctt gccaccttgc catgttgccg gattgctcca tgctccattt
     5641 gctacagaag ttactatctg ccctgtaccg gcacttgaca atgtaccgtg attaattcct
     5701 gcgcctgcta catcacctgc tattgtagct gtgccacccc caggggattt acgcagtata
     5761 ttcccggata cattacttgt tgatgcacga gcataaatat tggaaattat taattcgtca
     5821 gcctttggga ttccactgac agaaattgtt actgttatgg ttgttaatgt tacagcaatt
     5881 gatgctgttg taatatttct tgatgctaaa aaagaaacat ttcctgatcc gggctgaaac
     5941 ataaatccgg taggtgcact taggattaag gttttattcg actgaatagc aaaatcgcca
     6001 ttggcacctt cggtaataac aatattacca atgggaagaa atgccgatgg tgtgacattt
     6061 aggcaattac cacttgttgc tggcgaaacg gttactgcgg cattaacact gacatttaca
     6121 aaaaacaaca tcaggattaa ataactaatt cgggacataa ctgtagtttt atcccgtttt
     6181 ctacgataaa attcaaaaaa tgttccgaaa aaatgctgta aaaactactc tgaaaattta
     6241 tgaactaatg taacttttct cttttcagca gataagctaa taaaacctga tgataacctt
     6301 catggatgtc tgcctgaaca aaatcaattt tatattgcgc acatttatgt acaagcatgg
     6361 atttgtattc tttcatagct tcaatatact tttctctcac caagttactg tgcactttta
     6421 tctcttctcc ggtttcaaca tcaacaaagc ggtatggtct gttttcgaac tgaaagtcaa
     6481 gttcatgttt tttatcagta acatgaaaca gaatcacttc atgcttattg tgttttaaat
     6541 gttgaagcga tttaaacaaa gcttcattgc tatctatgtt ttcaaacata tcactgaaaa
     6601 taattatcag tgagcgtttg tgaatactct ctgctaactg atgtagtgca tctgcagttg
     6661 cagttttacg gtttacgtca gttttgtttt ctaataattt ttcgagatga gcaaaaacaa
     6721 gtttgtgatg agctgtactg gattttgcct gagtttgtac atcaatctgc tctgaaaaaa
     6781 ctgtcagccc tattgcatca cgctgttgct tcatcatgta taaaattgca gcagcgctat
     6841 aaacagaaaa tgtaatttta ttctgtaaac tctttccacc tttcacttca ggatagtgca
     6901 tagaggcgga gttatctatt acaatatggc agcgtagatt ggtctcctct tcaaatcgct
     6961 tgacaaaaag tttgtctgtt cttccaaaaa gcttccaatc aatatgtctt gttgactcac
     7021 ctgtgttata gagtctgtgt tctgcaaact ctacgctaaa accatgaaaa gggcttttat
     7081 gcaaaccggt aataaatccc tctaccacct gcttggcaag caattccaga tgtgagaggg
     7141 attgaacttc ctgatgatta accggcaaaa tattgttcta taaaattaaa actacactaa
     7201 ttgtgtatca atttttttgg caagaatatt ttttggagct gcacctacct gcttatccac
     7261 aacctgtcca ttcttgaaaa ataaaattgt tggaatgttt ctaacgccaa attgcattgt
     7321 aacattagga ttgctgtcca catcaatttt tccgattact gcttttcctt cgtaatcttt
     7381 tgccaattcc tcaacaattg gtcctaccat acggcatggt ccacaccatt cagcccaaaa
     7441 gtcaaccaat acaggtttgt ctgatttcat tacaacttcc tcaaagttgc tgtctgtaat
     7501 ttctattgcc ataattatgc tatgtttttt atttgatatt tcagaacaat aatagtggtt
     7561 taaagtttta tgatttttaa agatattgat attttttcat ttttttaagt ctcgagctaa
     7621 tttttcgccc atcaatagct aaaactcatt gcttttgtaa cttaagttac tatttaatga
     7681 gacttacacg ctctgaaatt gagttcagtt ccttaaacaa ttctttattg tattctaatt
     7741 tcagctgatt tgactccaca tcaatactca gattttgatc attgtcacga atactgaatt
     7801 tgacagttag tttgccctta ctatttttcg aaatctcttt taactttttt atattcatct
     7861 cattgattga ctccgaacga aatgaaatca ttattccctt tgccattttg tttgccacct
     7921 catccagaaa atgaactgaa ttaattttta attcaaactc cttctcatta taaaatttcg
     7981 tctgtaccct tgctttgata aaaacaaaag catcgttatt cagtaggtgt ttgaatttgg
     8041 catactcttc agaaaaaaca gcaaactcaa aagaagcatt ataatcttca atagtaaaac
     8101 tgccaaaagg tttacctgtc ttgctgactc tatgattggc attggtgata atgccggcaa
     8161 aatttacatc ccggttatta agtagtttga gttcgttaag ttgttcgaca gagatattac
     8221 aaaaagtttt tatttcttct ttataatcat caagtggatg accggaaaga taaattccga
     8281 ttatttcttt ctcctttttt aattgttcca gattactaaa atgttctgtc ggaataattt
     8341 taggcttaga tgcatcaaca ccacctaaat cgccaaacaa ggtgtgttga gtgctgcttt
     8401 ttg
//
LOCUS       DAORQT010000054         8346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1209_length_8346_cov_6.598722, whole genome shotgun sequence.
ACCESSION   DAORQT010000054 DAORQT010000000
VERSION     DAORQT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_8346_cov_6.598722"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1037
                     /gene="pdxA"
                     /locus_tag="PK134_03265"
     CDS             3..1037
                     /gene="pdxA"
                     /locus_tag="PK134_03265"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRB37898.1"
                     /translation="MGITIGDLNGVGIEVIIKTFADSRMLSVCTPVIYGSSKVISYHR
                     KSLHQQEFNFNTIRTAEEASHKKTNVINCWEEDVKVELGKPSTIAGTYAFKSLEAAGK
                     DVLDKKIDAIVTAPIDKSTIHSDAFPFKGHTEYFAKLCGNAEHVMLMVSDKLRVGLIT
                     GHVALKDVPAQLTKEKIIAKTKLVHQSLRSDFGLQKPRIAILALNPHAGDNGLMGKEE
                     QEIITPAISEMYNAGIGAFGPYPADGFFGSSRFKSFDAIMAIYHDQGLVPFKALAFST
                     GVNYTAGLPIIRTSPDHGTAYDLAGKNQASEDSFREAVYLACDIAKQRQITSEITANP
                     LKFSRMSADR"
     assembly_gap    1258..1286
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1336..3375
                     /locus_tag="PK134_03270"
     CDS             1336..3375
                     /locus_tag="PK134_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37899.1"
                     /translation="MKNLIAIIFCLLIFETVNAQWTQTNGPEGGDISSIVTDGTNLYA
                     GTYYGGVYRSSNNGQMWTAINNGLTHIRVGTLWLNGADLFAVTDGGLYVTSNNGNSWS
                     LINIVLPPFTSVTTFAANATNLFIGTSDNGVYTSTNNGVSWSVANSGLPLIQINSIAI
                     SGNNIFAVTQFQGVYLSTNNGVSWVAVNTGLTDLHISHLAIDAGNIFAGSIYGGVFLS
                     TDTGNTWNALNNGLTNLHVSAIAASGANVFAGTAGGGVFFSSDTGNTWTSANNGLTSS
                     TIYSFLINNSDIFVGTSADGLFLSTNNGASWNAQNTGLASSFVHSLAVSGSNLFAGSN
                     YKGMYRSTNNGNSWSQINNGLTSTFINSILATTNDLFVGTVNGLFMSADTGNTWVQSD
                     SGITNKFIIAVAKLGSHLIAASYLNEVYLSVNNGSTWNLINNGLPTGGSSSLVISGSN
                     IFLGTYQNGVFLSTDTGNTWNAVNNGLTNLHVSSLAISGTNIFAGTDSNGVFFSSNNG
                     NSWNAVNNGLPTTSIITCLAINGTNILAATMQDGVYFSNNNGTTWSAVNNGLTNKIVT
                     DLTISGINFFAATQGNGVFLNSNLLTDITENTNYYSSINIYPNPALSQVVIEFNHVID
                     NGKIQMFNTFGNKIIEENIISESKKVINLNNISNGIYFMKVFDGKNSNCKKLIVE"
     gene            3928..4659
                     /locus_tag="PK134_03275"
     CDS             3928..4659
                     /locus_tag="PK134_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37900.1"
                     /translation="MKKLLQFSFILATYLSSGQTAFEKMFKPSGVSDIFLTYYDVIKS
                     PDDSGYYTLSTFGGPDSGAILSLVGNDGILKWSKSYQRVTAIFKTFGLSVLPLSDSTL
                     MLNLYDDGRTIQLKTSYSGTMLGAKKLNYISSTVYFQEEKNNAIYGVGRVSANGVNGG
                     LFCKMDLDGNIIWSRMMNGGTSRRIELRALTLTSDGGFLLAGTRQIYGTILPASTILI
                     KTDSIGNITWQRQLNYVNKILFLLP"
     gene            4683..5501
                     /locus_tag="PK134_03280"
     CDS             4683..5501
                     /locus_tag="PK134_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37901.1"
                     /translation="MFAVATAFNGSAQFLKTDSVGNALWNVGFTDSTFYNLFFDQITP
                     DSGNTFVTAGNFDIFNPSINAAARIDSAGNLLSMHQYNNYSNCHSYSHYFNPQTGLAM
                     FGYGGGFNNFGFYFCKTDSVLTTPCMSTPSTFTTYSYSVTDSATSILSQPVSLVSIDV
                     LAQMSVNSISFTDSSLCPLVSSVPSFQNQNTLQVLNADVSLYPNPFSSQLTFSLASNE
                     KTTASLYNFVGQRVLQQTFSNSTTINTEQLPEGIYYYALHNYKGVFKTGKVIKQ"
     gene            complement(5872..6705)
                     /locus_tag="PK134_03285"
     CDS             complement(5872..6705)
                     /locus_tag="PK134_03285"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRB37902.1"
                     /translation="MKISVLGLQFDSALKNVFSMLSIALICLMQPSISEGQEKLRKVE
                     NQAFTYGEVLEYRVHYGIIDAGEAKLAVMPDKKTIGPREVYHVVGTGQTKGAFDWFFK
                     VRDRYESFIDAQAIIPWLFVRRVYEGGYKINQDVTFNQVKNLAISKKATIATPQNVQD
                     LISAFYYARTIDFSNAQPGALYDVDAYLDDAIIPVKFKYVGKENISTKFGTFRCLKFH
                     PQLLEGRVFKNKEDMTLWVSDDENKIPIRAEAKILVGSVKMDIKSFSGLANETLSLLK
                     K"
     gene            complement(6736..>6955)
                     /locus_tag="PK134_03290"
     CDS             complement(6736..>6955)
                     /locus_tag="PK134_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37903.1"
                     /translation="KPILYGISRTFMDHFGLKSAKDLPKLKDVILDENEIGTPADMQY
                     AENDNAEGVSITLGTQEGNTDKTDDVAE"
     assembly_gap    6956..6976
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6977..7438)
                     /locus_tag="PK134_03295"
     CDS             complement(<6977..7438)
                     /locus_tag="PK134_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRB37904.1"
                     /translation="MSRFRLKSRVKMENLKQHIEALIFTSEQSITANEIAAALKASFG
                     WELSNENIRQSISELINKYSADDYSFELVEIAGGFRFLSKKEYYGVVNTMLQINSKKR
                     LTTAALETLAIVAYKQPVSKSEIENIRGVNCDYSIQKLLEKDLITIQGKGDG"
     gene            complement(7485..>8346)
                     /locus_tag="PK134_03300"
     CDS             complement(7485..>8346)
                     /locus_tag="PK134_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRB37905.1"
                     /translation="GGLLYFFYSQIVSFAEDLPLLQAKLLEKVEFLQRFVNETFRITK
                     EQQNVLVKSKLQENIADSGLLLLNLFSSTGTFIANFALVTVYVFFLTLYKKKFKQFIE
                     AIVSDGKHNSYFQIVKKVAGVSQNYLKGIFLDVLILSILNSTGFLLLGIEHAVLFGVL
                     ASVLNIIPYIGVLIGSVLPVLMALLTKDEIGYAIGAAGVCVTVQFIDNNFITPYVVGS
                     SVSINPLTATIALIISASVWGLAGMVLCLPLTGMLKVVCDHVEGLKPIGFLIGEEVDY
                     RKNKFKLGRK"
BASE COUNT         2646 a         1584 c         1374 g         2692 t
ORIGIN      
        1 aaataggaat caccattggc gacctgaatg gtgttggaat agaagtcatc attaaaactt
       61 ttgccgacag cagaatgctc tctgtatgta cacctgtcat ctatggttct tcaaaagtta
      121 tttcctatca tagaaaatca ctgcatcagc aggaatttaa tttcaacacc atccgtactg
      181 ccgaagaagc aagtcacaaa aagaccaatg tcatcaactg ctgggaagaa gatgttaaag
      241 ttgaattggg aaaaccatca accattgcgg gaacctatgc attcaaatca ctcgaagctg
      301 caggcaaaga tgtgctcgat aaaaaaattg atgccatagt tacagcgccc attgacaaaa
      361 gcaccattca cagcgatgca tttcctttca aagggcatac agaatatttt gcaaagctct
      421 gcggcaatgc cgaacacgtt atgcttatgg tgagcgacaa acttcgtgtt ggactcatca
      481 caggtcacgt agctttaaag gatgtgcctg cacagcttac caaagaaaaa attattgcaa
      541 agaccaaatt ggtacatcag tcacttcgca gcgactttgg tttacaaaag ccacgtattg
      601 ccattcttgc actcaaccca catgctggcg ataatggttt aatgggtaaa gaagaacagg
      661 aaatcatcac ccctgccatc agtgaaatgt acaatgcagg cattggtgcc tttggaccct
      721 accctgccga tggttttttt ggatcttcgc gctttaaaag tttcgatgcc atcatggcca
      781 tctatcacga tcagggattg gtaccattca aagcattggc attctcaaca ggggtcaact
      841 acacagcagg tcttccaatc atccgcacct cgcccgacca tggcactgcc tacgatctgg
      901 caggaaaaaa tcaggcatca gaagattcat tccgtgaggc tgtctatctt gcctgcgaca
      961 ttgcaaaaca acgacaaatc acaagcgaaa taactgcaaa ccctttaaaa ttttcgcgca
     1021 tgtctgctga taggtagtat agaaaaagtt aagtagcaaa cctgtgtgta tatactatca
     1081 cattggcact tcatacttat taaattgcct aacacctcgc tattgttttt ttaaatagtt
     1141 tttttactac ttttgaacta atgaatagtt ttaattctga tagtcattgg attgataact
     1201 atcggcccga atgatgttat ctgatattgt ttagcggcag tgtaatttca tgttgatnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnntgtt gattgcaagt aattaatttt tgtaaactgt
     1321 gtaaaacaaa atataatgaa aaatctgatt gcaattattt tttgcttact tatttttgaa
     1381 actgtaaatg cacagtggac acagacaaat ggacctgaag gtggggatat atcatcaata
     1441 gtaactgatg gaacaaatct ttatgcaggt acttattatg gtggtgtata taggtcttca
     1501 aacaatggtc agatgtggac agctataaat aatggcttga cacatattcg tgttggaaca
     1561 ctttggttaa atggtgcaga tttatttgcg gttacagatg gaggactata tgtgacatcc
     1621 aacaatggaa attcgtggtc attaataaat attgttttac caccattcac ttctgttaca
     1681 acatttgccg caaatgcaac aaaccttttt attggcactt cagataatgg cgtgtatacg
     1741 tcaacgaata atggagtttc ttggtcggtg gctaattcag ggctaccatt aattcaaata
     1801 aactcaattg caataagtgg aaataatatt tttgctgtaa cacaatttca aggtgtttat
     1861 ttatcaacca acaatggtgt ttcatgggta gctgttaata caggtttgac ggatttacat
     1921 attagtcatt tagcaataga tgctggaaat atttttgcag gttcaatcta tggcggtgtt
     1981 tttctatcga ctgatactgg aaatacatgg aatgccttaa ataatggttt aacaaatctg
     2041 cacgtttctg caatcgcagc aagtggtgca aatgtttttg caggaactgc cggaggaggt
     2101 gtattttttt catctgacac aggaaataca tggacttcgg caaataatgg cttaacaagt
     2161 tcaacaattt attcatttct tataaataat tcagacatct ttgttggcac ttctgcagat
     2221 ggtttatttc tatcaactaa taatggtgct tcctggaatg cacaaaatac aggcttggca
     2281 agttctttcg tacattcatt agctgtgtca gggtcaaatt tatttgcagg aagtaattat
     2341 aagggaatgt atcgctctac taataatgga aattcttgga gccagattaa taatggttta
     2401 accagcactt ttattaattc tatcctagct actactaatg acctttttgt tggaacagtt
     2461 aacggacttt ttatgtctgc tgacactgga aatacttggg ttcaatccga tagtggtatt
     2521 acaaataaat ttataatagc tgttgctaaa ttaggttctc atcttattgc tgcatcttat
     2581 ttaaatgaag tctatttatc tgttaataat ggttcaacat ggaatctgat aaacaatggt
     2641 ttaccaacag gcggctcttc aagtttagta ataagtggct caaacatttt tttaggaact
     2701 tatcaaaacg gtgtttttct ttcaactgat acaggcaata catggaatgc ggttaataat
     2761 ggtttaacaa atctgcatgt ttcttcttta gctattagcg ggacaaatat ttttgcaggt
     2821 actgatagta atggtgtttt cttttcatcc aataatggca actcgtggaa tgcagtaaat
     2881 aatggtttac caaccacatc aattattact tgtcttgcga ttaatggaac aaatatttta
     2941 gcagccacaa tgcaagatgg tgtgtatttt tctaacaaca acggcactac atggtcagct
     3001 gtgaataatg gcctaacaaa taaaattgtt acggatttaa caataagcgg aataaatttt
     3061 tttgctgcta cacaagggaa tggagttttt ctcaattcaa acttattgac ggatattaca
     3121 gaaaacacaa attattattc ttccatcaac atttacccca accctgcatt aagtcaggtt
     3181 gtaattgaat tcaatcatgt aattgacaat gggaaaattc agatgttcaa tactttcggc
     3241 aataagatta ttgaagaaaa tattataagt gaatcaaaaa aagttatcaa tttgaataat
     3301 atatcaaatg gaatttattt catgaaagtg tttgatggaa aaaatagcaa ctgcaagaaa
     3361 ttaattgttg agtaatttat ttctcccatc tggctcaagt ctttcgactt gtgcttaatt
     3421 atcattaaaa aacttatatt actattgtgt tatttaatta ccaataataa ggttgtacct
     3481 atggcacttt aaaagttttt gatatctatt tttccaccaa aagtttgtcg ctcaaagact
     3541 gtttttttta cttatcaaac aacattctaa accttccaac attgcaacct tctgatttca
     3601 cctgtcaagt agcacagccc ctgacagagt gaattaacag aaacatatac ttaattttta
     3661 tcccctcaaa ttctcttcga tattacttcg cgaaactctg tagaaataac acaaacttta
     3721 aaaacattat aaccttcaaa ctttctttat atcctcaaaa attaaaaata taattatgca
     3781 ctatttttat aaaactttga gatgctataa gattgaaaag tagtatcgtt tgcttggttt
     3841 taaaaatcaa acttcgtaaa tttgtgagtt acactgacag tgttaaaaca caccactaca
     3901 gtgaatcgtt tatcgaaaat ttagacaatg aaaaaacttt tacaattcag ttttattctt
     3961 gcaacatact tgtccagcgg acagacggct ttcgaaaaaa tgttcaaacc cagtggtgtt
     4021 tctgacattt ttctcactta ttatgatgtt ataaaatcgc ccgatgattc ggggtattat
     4081 actctcagta cttttggagg tccagattcg ggtgcaatac tttctcttgt gggtaatgac
     4141 ggcatactga aatggagtaa atcttatcaa agagttaccg caatttttaa aacattcggg
     4201 ctatctgttc tacctctttc agatagcacc ttaatgctta acctctatga cgatggccgc
     4261 actattcaac ttaaaacaag ttattcaggt accatgttag gtgccaaaaa acttaactac
     4321 atttcatcaa ctgtttattt tcaggaagaa aaaaataatg ctatttatgg agtaggacgt
     4381 gtttcagcaa acggagttaa tggagggcta ttctgtaaaa tggatttaga tggcaacatt
     4441 atttggtcga gaatgatgaa cggaggtaca tcacgcagaa ttgagcttag ggcattaact
     4501 ctaaccagcg atggtggttt tctccttgca ggaacccggc aaatttatgg cacaatatta
     4561 cccgcttcaa ccatacttat caaaaccgac agtattggca atattacatg gcaacgccag
     4621 cttaattatg tcaataaaat attattcctt cttccgtagt agaaacccat gaccatggct
     4681 ttatgtttgc cgtagcaacc gcctttaacg gctctgcaca gtttttaaaa acggatagtg
     4741 tcggaaatgc gctatggaat gttggtttca cagattccac tttttacaat cttttcttcg
     4801 accaaataac accggattca ggcaacactt ttgttacagc aggtaatttt gacattttta
     4861 atccttcgat aaatgcagca gcaagaattg atagtgcagg aaacctgtta tccatgcacc
     4921 agtataataa ttactcaaat tgtcattcat acagccatta cttcaaccca caaaccggtt
     4981 tagcgatgtt tggttatgga ggtggcttca ataattttgg attttacttt tgcaaaactg
     5041 atagcgttct aacaacacct tgtatgagca caccatccac ctttacaact tattcgtatt
     5101 cggtaaccga tagtgccaca agcattttat ctcaaccggt aagccttgta agtattgatg
     5161 tgctggcaca aatgtcagta aactcaattt catttactga cagcagttta tgccctttag
     5221 tgtcgtctgt tccatctttt caaaatcaaa atactcttca ggttttaaat gctgatgtaa
     5281 gtttatatcc aaatcccttt tccagtcaac ttactttttc tcttgccagc aacgaaaaaa
     5341 caacagcgtc tctttataac tttgttggac aacgagtttt gcaacagaca ttttcaaact
     5401 ccacgactat aaatacagag cagcttccag aaggtattta ttattatgcg ttgcacaact
     5461 acaagggtgt gtttaaaaca ggtaaagtga taaagcaatg aaagctagta atttcagcta
     5521 atatgtttta gtttacaaaa gttagcaact tagtaaactg agtgggaatt cacctttgta
     5581 cataaacacc gactaacaat ttcttccttt tgtcaagtag ttaactttat aactgtcaag
     5641 ttgcaaaacc ccagattgtg taatttaaca aacacttttt ttccgcatct ctgtctctac
     5701 aattttacat ctgaatactc tgtggaaacc aacatcattc aaaaattcaa aaacattatc
     5761 aaattatcta atcatcaaac ctgtcagccg tggctgatta tcaaataaat taacttctct
     5821 caacaaatca cacttagata tttattaaag ttagttcgaa atgttattaa actatttctt
     5881 caacaacgat agtgtttcgt tggcaagtcc tgaaaagctt ttaatgtcca ttttaacaga
     5941 tccaactaaa atttttgctt ctgctcgtat aggaatttta ttctcatcat cacttaccca
     6001 gagagtcatg tcttccttgt ttttaaaaac acggccttcc aacaattgcg ggtgaaactt
     6061 caaacaacgg aaagtgccaa acttagtact gatattttct tttcctacat atttgaattt
     6121 tacaggaata atggcgtcat ctaaatacgc atcaacatcg tataaggcac cgggttgggc
     6181 attgctgaag tcaattgtgc gtgcataata aaatgcagaa atcaaatcct gaacattctg
     6241 tggtgtggca atggttgcct tcttgcttat ggctaaattt tttacctgat taaaagtaac
     6301 atcctgattg attttatatc caccctcata aacccttctg acaaacaacc aagggataat
     6361 tgcctgtgca tctataaaac tttcataacg gtctcgcact ttaaaaaacc agtcgaatgc
     6421 tccttttgtt tgtcctgttc caactacatg atacacttcc cgaggaccta tagttttttt
     6481 atcaggcatt acagcaagct ttgcttcgcc tgcatctatt attccgtaat gtactcgata
     6541 ttccaaaact tcgccataag taaatgcctg attttctacc ttacgtaatt tctcctgacc
     6601 ttcactgata cttggttgca ttaaacaaat gagtgcaatt gataacatgc tgaaaacatt
     6661 ttttaatgcc gaatcaaatt gcaagccaag tacagaaatt ttcacctcaa aaaatgaatt
     6721 tcaattaaag actgattatt ctgcaacgtc atcggtcttg tctgtattgc cttcctgtgt
     6781 tcctaaggta atacttacac cttcggcatt gtcgttctct gcatactgca tatcggcagg
     6841 tgttccaatc tcgttctcat caagaattac atcttttaac tttggtaaat cttttgctga
     6901 ttttaaaccg aaatgatcca tgaatgttcg gctgatacca tacaaaattg gttttnnnnn
     6961 nnnnnnnnnn nnnnnngcca tcaccttttc cctgaatggt aattaaatct ttctctaata
     7021 atttctgaat ggaataatcg cagttaacgc ctctgatgtt ttcaatttct gattttgaaa
     7081 caggttgctt gtatgcaact atagcaagcg tttccaatgc tgctgttgta agtcgctttt
     7141 tagagtttat ctgcaacatg gtgttcacta caccgtaata ttccttctta cttagaaaac
     7201 gaaatccacc ggcaatttct accaactcaa aagaataatc atcagcagaa tatttgttga
     7261 ttaattcaga aatactttgt cttatatttt cattagaaag ctcccatccg aaacttgctt
     7321 tcaaagcagc agcaatttca tttgcagtga tactttgttc tgaagtgaaa attaatgctt
     7381 caatatgctg tttaagattc tccattttta ctcttgattt taatctgaat cggctcatgc
     7441 caattcgcta taaattgcaa aattagcatt tcatttcaac agacctattt tcgcccaagt
     7501 ttaaatttat tcttcctata atccacctcc tcacctatca aaaatccaat aggttttaaa
     7561 ccttctacat ggtcgcaaac tacctttaac atccctgtca gaggcaggca taaaaccatt
     7621 cctgccaagc cccacactga agcactgata attaacgcta ttgttgcagt gagtggattt
     7681 atacttacac tactgccaac tacataagga gtaataaaat tattgtcaat aaactgcaca
     7741 gtgacacaaa ctccggcagc tccaattgca tagcctattt catcttttgt gagcaatgcc
     7801 attagtaccg gtaaaacact tcctattaaa acaccaatgt agggtatgat gttgagaaca
     7861 gaagccagta ctccaaataa aacagcatgt tcaatgccca ataacaaaaa tcctgttgaa
     7921 ttaagaatgg ataaaatcaa aacatctaaa aaaatacctt taagatagtt ttgtgaaaca
     7981 ccagctactt tttttacaat ctgaaaatag gaattgtgtt ttccatcaga aacgattgct
     8041 tcaataaact gcttaaactt tttcttataa agtgtcagaa aaaaaacata aacagttact
     8101 aaggcaaaat tggcaataaa agttcctgtt gaagaaaaaa gattgagcaa cagtaatcca
     8161 ctatctgcaa tattttcctg caatttagat tttaccaaaa cattttgttg ctctttggta
     8221 attctgaatg tttcgtttac gaagcgctgc aaaaactcta ctttttcaag gagttttgcc
     8281 tgaagtaaag gcaaatcttc agcaaatgaa acaatttgcg aatagaagaa atacaaaagt
     8341 ccacca
//
LOCUS       DAORQT010000055         8248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1233_length_8248_cov_9.378250, whole genome shotgun sequence.
ACCESSION   DAORQT010000055 DAORQT010000000
VERSION     DAORQT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_8248_cov_9.378250"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..2805
                     /locus_tag="PK134_03305"
     CDS             376..2805
                     /locus_tag="PK134_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB37906.1"
                     /translation="MIRKYSLFLLLFLQIQILNAQDKVKRFTISGYVKDASTGEALIG
                     ANVYLRETMKGVQTNTYGYYAITVDASDSVTLMSSFVGYNDFQQRMLIVKDVKVNVNL
                     HNKYIETQEVVITAKKEDQNVNSTQMGTVQLDVEQIKTLPAFMGEVDILKTIQLLPGV
                     QSAGEGNTGFYVRGGGPDQNLILLDEALVYNASHLFGFFSVFNSDAINNVSLIKGGMP
                     ANYGGRLASVLDISLKEGNNKEFKAEGGLGPIASRLTLQGPIQKDKSSFIVSGRRTFI
                     DLFQRPPFVKKGSNAANNTYYFYDLNAKINYQLSDKDRLFLSGYFGRDVFNFKSADTG
                     FKFRIPWGNATAVLRWNHLFSDKLFVNTSIIFSDYNFAFNAKQESFSFNIFSGIRDYN
                     AKIDFSWFPIITHNVKFGVNYTYHRFTPTSAEASQDSTEFDLGGVNRLYAHEAAAYIN
                     DDWTISDKFSVSAGLRYSLFDFTGPFTRYVKNEDNETIDTIYYKTNETVGFYHGLEPR
                     ISAKFSLDKNSSIKASFTQNYQYVHLASFTSVTLPTDVWVPSSDRVKPQDGRLYAAGY
                     FRNFHQNMFETSVEVYYKTMRNQIEYREGAQADDDVKNNTDNNFVFGKGWSYGAEFFI
                     KKAKGKLNGWVGYTLSYTDRKFPDIMNGKTFPAKYDRRHDISVVVSYNASKRWTFGLT
                     WVYATGNATTLPVSRYFMFGPLDLQGVVSSGLPNMSNGQIYDVYGDRNSYRMAAYHRL
                     DLSATYIKKKTKRFESSWNFSIYNVYSRMNPYFIYFNNEVDKETGEFKIQAKQVSLFP
                     IIPSVTYNFKF"
     gene            2805..3689
                     /locus_tag="PK134_03310"
     CDS             2805..3689
                     /locus_tag="PK134_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRB37907.1"
                     /translation="MAMKINLIKSFLLIFSATNLFISCEKDITVNLPKQKQQYVVEGY
                     IENGLPAYVMLSKTADYFSPVDSAALQQYMVKNASIVISDGDITDTLLQVDPSIGYFY
                     FSPKIIGQTGKSYSLRIITPDNELLTAETKMLPAVPLDSVWFKIQEGKDSLGYVWAHL
                     KDPDTLGNCYRWFAKRLNKDKMFIAPSGSVFEDKFINGKGFDFAYNRGHLPNSTAVED
                     ENEEAGFFKAGDTIMVKFCTITEDSYKFWRQAESQAGSSGSPFGSPAVFPTNIKGGIG
                     IFEAFNPTFYTVIAKKPD"
     gene            complement(3697..4995)
                     /locus_tag="PK134_03315"
     CDS             complement(3697..4995)
                     /locus_tag="PK134_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB37908.1"
                     /translation="MIKVRLTVLICFFFVQAQCQQVFTLQQAIEKALNDNFSIRIARN
                     QSEILNNNATLGNAGMLPQVNLNGSYVKANNNVVQELSNGTSTEKQNAGQSTIAGSAV
                     LEWVLFDGLTMFSEYNKNSALSKQGKEQLKANIEDVIENVSKSYFTVVKEKLLLSSIN
                     ETLKFYADRLQIADSKYKIGSASKLDYLQSKVDYNAMLSQKLNKETDVNNAMVELNRL
                     LQYSADTLLQTEDTITFVTMPAMNDLITGLQQNTAMQVSKLNVEVNRLQLNSIKGLRL
                     PVITGLAGYYYNENRNDAGLFSLNRTNGPQFGITARYNLFNGFNNSRQISNTKLNYIN
                     SQIEFQQTRTNQIADLNNTWNAFQKSQEILNLETENTAAARENASVYLASYRLGSANL
                     LQLNQSQESLYDALVRLVNAQYVNKTTEIHLLRLAGKLIK"
     gene            complement(4988..8053)
                     /locus_tag="PK134_03320"
     CDS             complement(4988..8053)
                     /locus_tag="PK134_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB37909.1"
                     /translation="MDLSEISVKRPVLAIVMSLVILLFGVISYTFLGVREYPSIDPPI
                     INVRTSYPGANADIIESQITEPLEKVINGIQGIRSISSTSSQGTSNITVEFNLNSDLE
                     AAANDVRDKVSQAVRTLPRDIDGLPVVTKSDANADAIISLTVQSNTRNTLDLSDYAEN
                     VIGQQLITIPGVSTLQIWGQRKYAMRLRIYPDKLAAYKLTVQDIQNALNRENVELPAG
                     KIAGDNTELVVRTTGQLHSEDDFNNMIIFNDGDQLIRFKDVGLAELGPENEETILRES
                     GIPMVGIALVPQPGSNYIEIADEFYKRLEKIKVDLPSDIKIGIALDNTTFVKKSITEV
                     EETLLIAITLVVIIIYLFFRDWLVAIRPLIDIPVSLVGAMFIMYIAGFSINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKIEAGLSPNKAAIEGTKEIFFAVVSTSVTLAAVFLPVV
                     FMEGFVGRLFREFGVVIAGAVLISAFVSLTLTPMLNARLVRKDKKHSKFYEKTEPFFQ
                     RMNQAYENSLKWFMEARWRAWTILGSAVALIFILGRSLPSELSPLEDRNWLRLLITAP
                     EGASYEYTDNFIVKISDVIEDSIPEKNGLLTVTAPGFTGAGAVNSGFARIVLTTPDKR
                     ERSQQEIADQLSGITRKMTDARVFVLQEQSLSAGGSRSALPVQFVLQNQEFDKLREVV
                     PAFLDKANKSPVFQGVDVNLKFNKPELNVSIDRAKANALGVSVDDIAQTLQLAFSGQR
                     FGYFIMQGKQYQVIGQVDRENRDVPYDLSKLYVKTNSGGLVQLDNLIEIKEISSPPQL
                     YHFNRYQSATISAGLAPGYTIGDGINEMKHIGDSLLDESFSTDLTGPSRDFAESSSNV
                     WFAFVLAIILIYLVLAAQFESFVDPFIIMITVPLAICGALISLWYFNQTLNIFSQIGI
                     IMLIGLVTKNGILIVEFINQLREKGVERSEAIIHGSVSRFRPILMTSLATSLGALPIA
                     LALGAGAKSRMSMGIVIVGGVLFSLIFTLYIIPAMYSVFSRKKEMVSYD"
     gene            complement(8058..>8248)
                     /locus_tag="PK134_03325"
     CDS             complement(8058..>8248)
                     /locus_tag="PK134_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HRB37910.1"
                     /translation="ILKGKKVFISKNGKADEKIIETGERTADMIEVTSGLTAGDTVLT
                     TGILQVRKGSDVKFSEVK"
BASE COUNT         2642 a         1572 c         1399 g         2635 t
ORIGIN      
        1 cataattctg aataatgcca acatctcctt gtgcaaataa tgctacaggg cgtgttactg
       61 aaaacggccc ggacaatttt ttagcaagtg taaaatttat cttgctcaaa tcctgagcga
      121 atgcaccctg aagaataaat aataaaaata ctaagagtga aatatgtggt tttttcatta
      181 ctattttatt ggtcaacaat tttaaataaa atattctgtt tagataatga ggggcaatac
      241 aattatcaac aaatttacaa taaaaggttt ttttacaata tttatttcag attaaaaata
      301 tatgttaaca ttttaagtat ttgaatctga atttaagaat gaaatgataa gtttgcaacc
      361 ccgtataatc aatttatgat cagaaaatat tcgctctttt tacttctttt tctacaaatt
      421 caaatcttaa acgctcagga caaagtaaaa cggtttacta ttagcggtta tgttaaagat
      481 gcctctacag gcgaagcatt aattggagct aatgtatatc tgcgtgaaac gatgaaaggt
      541 gttcagacta acacttatgg ctactatgca attaccgttg atgcctctga tagtgttaca
      601 ctgatgagct cttttgtagg atataacgat tttcagcaac gaatgcttat tgttaaagat
      661 gtcaaagtaa acgtgaatct gcacaacaaa tacattgaaa cgcaagaagt agttatcact
      721 gccaagaaag aagatcagaa tgttaacagc acgcaaatgg gtacagttca acttgatgtg
      781 gagcagataa aaacactgcc tgcatttatg ggtgaagtgg atattttaaa aactattcaa
      841 ctgctgcccg gagtgcaatc tgccggtgag gggaacacag gtttttatgt tcgcggtggt
      901 ggccccgatc aaaatttaat attattggat gaagcattag tttataatgc ctcacattta
      961 tttggttttt tttcggtatt taattccgat gccatcaata acgtttcatt gattaaaggt
     1021 ggcatgcctg caaactatgg cgggcgactt gcatctgtat tagacatttc gcttaaagaa
     1081 ggaaacaaca aagagtttaa agcggaaggt ggattaggac ccattgcctc acgacttaca
     1141 ttgcaaggcc cgattcaaaa agataaatca tcattcattg tatcaggaag gcgtacattt
     1201 attgatctgt ttcaacgtcc accgtttgta aaaaaaggtt ccaatgctgc taacaacacc
     1261 tattacttct atgatttgaa tgctaaaata aactatcagc tttcagataa agacagactt
     1321 tttttaagcg gctactttgg cagagatgtg tttaacttca aaagtgccga tacaggattt
     1381 aagttcagaa tcccgtgggg aaatgctact gccgtattac gctggaatca tttattcagt
     1441 gacaagttat ttgtaaacac ctcaattatt ttttctgatt ataactttgc tttcaatgca
     1501 aaacaggaaa gttttagttt caatattttt tcaggcatca gagattataa tgcaaaaatt
     1561 gactttagct ggtttcctat tatcacacac aatgttaagt ttggtgtaaa ctacacctac
     1621 catcgcttta ctcctacaag tgcagaggcc agtcaggatt caacagaatt tgatttgggt
     1681 ggagtaaaca gactctatgc acatgaagct gctgcctaca ttaatgatga ctggacaatt
     1741 agtgataaat tttctgtaag tgctggctta cgttattcac ttttcgattt tacagggcct
     1801 tttacacgct atgttaaaaa cgaagacaat gaaactattg atactattta ctataaaaca
     1861 aacgaaactg ttggtttcta tcacggactt gagcctcgca tttcagctaa attttcgtta
     1921 gataaaaact cttccataaa agcatcattc acacaaaact atcaatatgt gcacttggca
     1981 tcgtttactt ctgttacgct gcctacagat gtttgggtac ccagcagcga cagagtaaaa
     2041 ccacaagacg gaagactgta tgccgcagga tatttcagaa actttcatca aaatatgttt
     2101 gaaacatctg tagaagttta ttacaaaacc atgcgcaatc aaattgagta tcgtgaaggt
     2161 gcacaggctg atgatgatgt gaaaaataat accgataata attttgtatt tggtaaagga
     2221 tggagttatg gtgctgaatt ctttatcaaa aaagcaaaag gaaaactaaa tggatgggtt
     2281 ggttacacac ttagctatac agatagaaaa tttcctgata ttatgaatgg aaaaacattt
     2341 ccggcaaagt atgacaggcg acatgatatt tctgttgtgg tgtcttataa tgcaagcaaa
     2401 cgttggacat ttggtttgac ttgggtttat gcaacaggta atgccactac tctgcctgtt
     2461 tcaagatatt ttatgtttgg tcctttagat ttacaaggtg ttgtatcaag tggattaccc
     2521 aatatgtcaa acggacagat atatgatgtt tatggcgaca gaaattctta cagaatggca
     2581 gcctatcatc gtttagattt atctgcaacc tatatcaaga aaaagacaaa acgctttgaa
     2641 tcaagttgga attttagcat ctacaatgtt tacagcagaa tgaaccccta ttttatttat
     2701 tttaataatg aagttgataa agaaacaggt gaatttaaaa ttcaggcaaa acaagtttcg
     2761 ctgtttccaa ttattccttc tgttacttat aattttaaat tctgatggcg atgaaaataa
     2821 atcttataaa atcatttcta ttaatttttt ctgcaacaaa tttattcatc tcttgcgaaa
     2881 aagatattac agttaatctc ccaaaacaaa aacaacaata tgttgtagaa ggttatattg
     2941 aaaatgggtt accagcctat gtcatgcttt ctaaaacagc agattatttt tcgccagttg
     3001 attccgcagc actgcaacaa tacatggtta aaaatgcttc cattgttatc tctgatggtg
     3061 atattactga tacactacta caggttgacc catcaatagg ctatttttat ttttcaccaa
     3121 aaattattgg gcaaacaggt aaaagctatt cgttacgcat cattacaccc gacaatgaat
     3181 tacttactgc tgaaactaaa atgcttcctg ccgtaccatt agacagtgta tggtttaaaa
     3241 ttcaggaagg gaaagattca ttaggttatg tatgggcaca tttaaaagat cctgacacat
     3301 taggtaattg ctatcggtgg tttgccaaac gattaaataa agataaaatg tttattgcac
     3361 catcaggctc ggtgtttgaa gataaattta ttaatggaaa aggatttgat tttgcatata
     3421 accggggaca tttgcctaac agtacagcgg ttgaagatga gaatgaagaa gcaggattct
     3481 ttaaagccgg tgataccatt atggtaaaat tctgcacaat aacagaagat agctataagt
     3541 tttggcgtca ggcagaatca caggcaggaa gtagtggcag tccttttggt tcgccagcag
     3601 tttttcctac aaatattaaa ggcggtattg gaatttttga agcttttaac ccgaccttct
     3661 atacagtaat tgctaagaag cctgattaaa ttttctctat ttaattaatt tgcctgccaa
     3721 acgcagtaaa tgtatttcag tagttttgtt aacatactgt gcattaacca accgcactaa
     3781 ggcatcatat aaactttctt gtgattgatt taattgcaaa agatttgcac taccaagacg
     3841 ataactggca agatacacac ttgcattttc acgggcagca gcagtattct cagtttctaa
     3901 attaagtatc tcctgagatt tttgaaatgc attccatgta ttatttaaat cagcaatttg
     3961 gttggttcgt gtttgctgaa attcaatttg cgaattgata taattcaatt ttgtattact
     4021 gatttgtctt gaattattga aaccgttaaa caaattataa cgtgccgtga ttccaaactg
     4081 tggtccatta gttctgttca aagaaaacaa tcctgcatcg ttgcggtttt cattataata
     4141 ataccctgca agacctgtaa ttacaggcaa acgtaatccc tttattgaat ttaactgtaa
     4201 acgatttact tctacattca gtttgctcac ttgcattgca gtgttctgtt gaagtccggt
     4261 tatcaaatca ttcattgcag gcatagttac aaaagtgatt gtatcttcag tttgcaataa
     4321 agtatcagca gaatattgta acaaacggtt taactcaacc attgcattat ttacgtctgt
     4381 ttccttattc aacttctgcg ataacattgc attgtaatca acttttgact gtaaataatc
     4441 cagtttagag gctgaaccaa ttttatattt actatctgca atctgaagtc tgtctgcata
     4501 aaatttcaag gtttcattaa tgctcgacaa caacaacttt tctttaacta cagtaaaata
     4561 tgatttactt acattctcaa taacatcttc aatgtttgcc ttcagttgct cttttccttg
     4621 ttttgagagt gctgaatttt tattatactc cgaaaacata gtaagtccat caaataagac
     4681 ccattccaaa actgcactac ctgcaattgt tgactgacct gcattttgtt tctctgtaga
     4741 tgtgccatta ctcaattcct gcaccacatt gttatttgcc ttaacatagc taccattcaa
     4801 atttacctga ggcaacattc ctgcattacc taatgttgca ttgttgttta gaatttcact
     4861 ctgatttcgg gcaatacgga ttgaaaaatt atcattgagg gccttttcaa ttgcctgttg
     4921 cagtgtaaac acctgttgac attgtgcctg tacaaagaaa aaacaaatca atactgttaa
     4981 tcgtacctta atcataactt accatttctt tttttcgtga aaaaacagaa tacattgccg
     5041 gaataatata caaagtaaat atcagagaga aaagtactcc accaacaatt acaattccca
     5101 ttgacatacg actctttgct cccgctccta aagctaatgc aatgggcagc gctcctaatg
     5161 aggtagcaag acttgtcatt aaaattggcc ggaaacgtga tacagaacca tgaataattg
     5221 cttcagatcg ttctactcct ttttcacgta gttgattaat aaactcaaca attagaattc
     5281 cattttttgt aacaaggcca atgagcataa tgataccaat ctgactgaaa atatttaatg
     5341 tctgattaaa ataccaaagt gaaatcaatg caccacaaat tgcaagtgga actgtaatca
     5401 tgataataaa tgggtcaaca aaactttcga actgtgctgc caacaccaaa tagattaaaa
     5461 taattgcaag tacaaatgca aaccaaacat ttgaactgct ctctgcaaaa tcgcgtgaag
     5521 gacctgttaa atcggtcgaa aaactttcat caagtaaact atcgccaata tgtttcattt
     5581 catttattcc atcaccaatt gtgtaacccg gtgcaagccc tgctgatatg gtggcagatt
     5641 gataacgatt aaaatgatat aactgtggtg gactactgat ttcttttatt tcaattaaat
     5701 tgtcaagctg aacaagacct ccactgtttg tttttacata aagttttgaa aggtcataag
     5761 gcacatcacg attttcacga tctacctgac cgatgacctg atactgcttg ccctgcatga
     5821 taaaatatcc aaatcgctgt ccgctgaaag ctaattgaag tgtttgtgca atgtcatcaa
     5881 cagatacccc taatgcattt gcctttgctc tgtcaataga aacatttaat tctggcttgt
     5941 tgaatttcag gttgacatcc acaccctgaa atacaggact tttatttgct ttatctaaaa
     6001 atgcaggcac aacctctctt agcttatcga attcctgatt ttggagaaca aactgaactg
     6061 gtaatgcact acgtgatcct cctgccgaca atgactgttc ctgcagaaca aatactcttg
     6121 catcagtcat cttgcgtgta attcccgaaa gttgatctgc aatttcttgt tgcgaacgtt
     6181 cacgtttatc tggtgttgta agtactattc gggcaaagcc tgaatttact gctcctgcac
     6241 cagtaaaacc cggtgcagtt actgttagca atccgttttt ctccggtata gaatcttcaa
     6301 taacatcaga aattttcacg ataaaattat ctgtatattc ataagacgct ccttccggtg
     6361 ctgtaattaa aagtcgcagc cagtttctgt cttctaaagg tgaaagttca cttggcaaac
     6421 tccttcctaa aataaaaatc aaagcaacgg ctgatcctaa aattgtccat gcacgccatc
     6481 ttgcttccat gaaccactta agagaatttt cataagcctg attcatccgt tgaaaaaacg
     6541 gctctgtctt ttcataaaat ttagaatgct ttttatcctt tctcaccaaa cgcgcattta
     6601 acattggtgt taaggttaat gaaacaaatg ctgaaataag tactgcacct gcaatgacca
     6661 caccaaactc cctgaagagt cgcccaacga atccttccat aaaaactaca ggtagaaata
     6721 ctgctgctaa cgttactgag gtactgacaa ctgcaaaaaa tatttctttt gttccttcaa
     6781 ttgcggcttt gttgggtgaa agtcctgctt caattttttt ataaatattt tctgtaacaa
     6841 caattccatc atctaccacc aatccggttg caagcactat cgctaaaagc gttagcacat
     6901 taattgaaaa tccagctatg tacataataa acatggctcc aaccaacgag actggaatat
     6961 ctatcaatgg acgaatggcc acaagccagt cacggaaaaa caaatagata atgattacaa
     7021 caagcgtaat ggcaattaaa agtgtctctt ccacctcggt aattgacttc tttacaaaag
     7081 ttgtgttgtc gagtgcaata ccaattttaa tatcagaagg caaatcaact tttatttttt
     7141 ccaacctttt ataaaattca tcagcaattt caatgtaatt acttcctggt tgtggcacaa
     7201 gagcaatacc caccatcgga atgcctgatt cccttagaat agtttcttca ttctccggcc
     7261 ctaattctgc aagacctaca tctttaaacc gaataagttg atcgccatca ttaaagataa
     7321 tcatattatt gaaatcgtct tcgctgtgaa gctgtcctgt agtccgtaca acaagttcag
     7381 tattatcacc tgctattttt cctgcaggca attccacatt ctccctattc aatgcatttt
     7441 gaatatcctg aacggtaagt ttatacgcag caagtttatc aggataaatt cttaaacgca
     7501 tagcgtattt acgctgtccc caaatctgca aggtgctaac acctggaatt gtaattaatt
     7561 gctgaccgat aacattctct gcatagtcac ttaagtcaag agtattccgg gtattactct
     7621 gaacggtaag cgaaatgatg gcatcggcat tagcatcaga tttagtaaca accggtaatc
     7681 catcaatgtc acgtggcaaa gtacgaacag cttgagaaac tttatctctt acgtcattgg
     7741 ctgctgcttc gagatcagaa ttcagattaa actcaacagt aatattactt gttccctgac
     7801 tgctagttga agaaattgaa cgaatgccct gaataccgtt tatgactttt tcaagtggtt
     7861 ctgttatctg cgactcaata atatcggcat tagcaccggg atacgatgtt cttacattaa
     7921 taattggtgg gtcaatagat ggatactctc tgacaccaag aaaagtataa ctgatgacac
     7981 cgaataacag tatcaccaac gacataacta tagcaagaac ggggcgtttt acacttatct
     8041 cactcagatc cataaaccta ttttacctca ctgaatttaa catcgcttcc ttttctaacc
     8101 tgcaaaattc ctgttgtcaa caccgtatca ccggcagtca aacctgaagt aacttctatc
     8161 atatctgctg ttcgctcacc tgtctcaatt attttttcgt ctgccttgcc atttttacta
     8221 atgaaaactt tcttgccttt taaaattg
//
LOCUS       DAORQT010000056         7918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1336_length_7918_cov_14.822841, whole genome shotgun sequence.
ACCESSION   DAORQT010000056 DAORQT010000000
VERSION     DAORQT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1336_length_7918_cov_14.822841"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..718
                     /locus_tag="PK134_03330"
     CDS             47..718
                     /locus_tag="PK134_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRB37911.1"
                     /translation="MSCISCKKLWSIPALEWLLILMTLTLGLLWTSCNEDRPLRTLPV
                     YGMQDEQNKSNHTIGSFSFIDQNGDTVTDKDYSNKIYVTDFFFTTCRSICPVMTTQMQ
                     RVFEHYKSNPDVMFLSYSVNPEYDTPAVLKEYADKHHADAKKWHFVTGNKQKIYDLAR
                     ISYLVTATKGDGGPDDFVHTQNFALIDKEKRIRGYYDGTDSTDVNRLITEIDLLLREY
                     KYADK"
     gene            715..2613
                     /locus_tag="PK134_03335"
     CDS             715..2613
                     /locus_tag="PK134_03335"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRB37912.1"
                     /translation="MMQKLLFFFILSPFLSFSQDNVHIHCKRIISDNSKLHSLNTVST
                     TGSDFDVKYNRFNWTINPDSVYISGNVFTLFEVKAPISKLEFDFSSALTIDSILYQST
                     LLPYTSNSDIITILLPGIISTGQLDSIEIFYHGVPPTSGFGSFNQSTHNGVPVLWTLS
                     EPFGASDWWPCKNSLTDKVDSIDVFVTTDTAYKVASNGKLISEIASGNQKITHWKSNY
                     PTASYLIAIAVTNYAAYSDYVPMAGGDSLQVLNYVYPENLSSAQTQTPDIIPIIQLYD
                     SLTIPYPFSHEKYGHAQFNWGGGMEHQTMSFMVNFSQDLMAHECAHQWFGDLVTCGSW
                     EDIWLNEGFATYFEGLTKEHYYPSLWYNWKKQKINNITSQPDGSVFCTDTNNVSRIFN
                     GRLTYNKGSYLLHMLRWQLGDSVFFNALTSYLNDTLLKYSFAKTPDLIAHLQNSSGQN
                     LSTFFNQWYYNQGYPSYHITWNQNNPSVWLKIDQTTSHPSVSFFEMSVPVRFYGNGTD
                     TTIVFNHTFSGQQFTASIPWQVDSVVFDPDLWILSANNLVTTGIETKDLSNENLQIYP
                     NPVRNFINVLCKTNCKYIITDASGKTISKGELHQKNNRIILNSKPGIYFLNTSDENRW
                     RSQSFIIQ"
     gene            2629..3888
                     /locus_tag="PK134_03340"
     CDS             2629..3888
                     /locus_tag="PK134_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRB37913.1"
                     /translation="MRIKTILITLLAIVNLRANAAQILIPMDDTQTNHLKAYGIAYWV
                     LQSGVEINWMLNYRGGSFMFIQNPKFESELKLRGVSYNVISEGQANQIVSEILDPQVN
                     QDVVKLEKVPKICVYSPKDKRPWDDAVTLALTYAEIPYDVVYDEEIVQGKLALYDWMH
                     LHHEDFTGQYGKFYNMYRNATWYQEQQRSMESMAKQLGFAKVSQMKLAVAKKIKEFVA
                     GGGFLFTMCSGTDTYDIALSAEGLDICESMYDGDGTSPNYNDHLDFSKTFAFRNYTLS
                     VNPLEYRFSNIDVTTTRRVPRDEDVFTLFDFSAKWDPVPTMLCQDHMQVIKGFMGQTT
                     AFNKNYIKPDVIIMGENKAANEAKYIHGDFGKGTWTFYGGHDPEDYQHMVGDPPTDLN
                     LHPNSPGYRLILNNVLFPAAKKKKQKT"
     gene            complement(3885..4718)
                     /locus_tag="PK134_03345"
     CDS             complement(3885..4718)
                     /locus_tag="PK134_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB37914.1"
                     /translation="MITRKQPVTMMYSKALIKPDGEVLNYSDTSSGTEVLVLIHGYPF
                     DQSIWKEQVIALSDHARIITYDVRGYGSSSKGKERFSIDLFADDIIFLLDSLQVSKFI
                     VCGLSMGGYIALNLVKRYPERIKGLVLYDTQCYADNAIGKAKRYENIELIRNGGKQKY
                     LEDMCKILFSPVTFQKNNEIIKQTLDLMMKADNEVLMQTLEAMAEREESCSNLSSIKF
                     PVLILCGEHDVLTPPEKSEFMQQQISGSLLHIIENSGHLGHLENKDAFNQHLNNFLKS
                     F"
     gene            complement(4733..6505)
                     /locus_tag="PK134_03350"
     CDS             complement(4733..6505)
                     /locus_tag="PK134_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRB37915.1"
                     /translation="MEVTRLIDLFPYQLSRFPKADALAGKQDGVWKKYATADIIETIN
                     NLSYGLLHSGIRPGDKIGIIANNRPEWNFVDHACLQIAVADVPLYPTISENDLKFTLN
                     QSTVRYLFVSNVDLYKKVSQIKSELPVLEKIYLFDRYQDLPHWSELIDLGLKHPKQEE
                     VKQIRDSIKPDDLATIIYTSGTTGNPKGVMLSHNNLVSNFKFCDVLCPFHESWKALSF
                     LPLNHVYERMLSYLYVYFGVSIYYAESLDTIADNLKEIKPQIFVTVPRLLEKVYDKIV
                     SKGAELKGIKKALFFWALNLGLRYELKGANGWWYEFQLRLANKLIFSKWREALGGNIQ
                     AIASGGAALQPRLARVFHAAKIPVLEGYGLTETSPVIAVNNFQPDSIKFGTVGTVLEN
                     VEVKIASDGEILCKGPNVMLGYYNSPELTAEVIDADGWFHTGDIGVFEDGKFLKITDR
                     KKEIFKTSGGKYIVPQMIENKLKESRFIEQAMVIGENQKFASAFIVPNFAFIRDWAAK
                     KNISVKSNAEIAASSEVKKRIAEEVEVVNKTLGHYETIKRFELLDREFSIERNEMTPK
                     LSLRRKIILENYKDKLAKIYKGTE"
     gene            6574..6957
                     /locus_tag="PK134_03355"
     CDS             6574..6957
                     /locus_tag="PK134_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRB37916.1"
                     /translation="MNYNDLLNKSKPLLIFDGSCAFCRSCIRFVKKYDIKLQFDFADN
                     NQNTTKLILAQYFINPESLNSVVLIEYNKLHIQSDAIIKALLLLGGRWKILARIMMVF
                     PNSFRNKVYNIIAANRNKLRIHCRV"
     gene            6954..>7918
                     /locus_tag="PK134_03360"
     CDS             6954..>7918
                     /locus_tag="PK134_03360"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRB37917.1"
                     /translation="MKTNFTEQTGIAYPIIMAPMFLVSNEAMMQSAIRTGIMGVFPTL
                     NYRKEGELDALVKRLHQFKAKKQGSFGVNLIVQKTNPLYNRHLKICVENKVPFYITSL
                     GSPREVIEAAHSYGAKVYCDVTNIAHAEKCAKLGCDGFIAVGQGAGGHAGPYAQTVLI
                     PTLRKHFPSIPVIAAGGIATGDGIAAMMALGATGVSIGTRYIASTEAEVSDEYKNSIL
                     NSGMDNIVMTERLSGTPCNIINTPYAKKIGYKQNWLEKWLSNNSTTKKYFKMMVQYKG
                     VKALEQAIKPGNYKTLWTAGQTVELIDDIINCDEITERLIKETHLS"
BASE COUNT         2572 a         1453 c         1361 g         2532 t
ORIGIN      
        1 gtaaagaaga ttttgttaaa gaaccactca cctaccttga agaaaaatga gttgtattag
       61 ttgtaagaaa ctttggtcaa ttccggcttt ggaatggctt ctcattttaa tgactcttac
      121 tttaggattg ttgtggacat catgcaatga agatcgccca ttgcggacat tgccggttta
      181 tggaatgcag gatgaacaaa ataaatccaa tcatacaata ggatcttttt cttttattga
      241 tcagaatgga gatacagtta cagataaaga ctattccaat aaaatatatg tgactgattt
      301 cttcttcacc acatgcagaa gtatttgtcc tgtaatgaca acacagatgc agcgtgtatt
      361 tgaacattac aaatcaaatc ctgatgttat gtttttatcg tattcagtaa atcctgaata
      421 tgatactcct gctgttttaa aagaatatgc agataagcat catgcagatg ccaagaaatg
      481 gcattttgtg accggcaaca aacaaaagat atatgacctt gcccgcattt cctatttagt
      541 aactgcaaca aaaggtgatg gcggtccgga tgactttgtg cacacacaaa actttgcgtt
      601 gatagacaaa gaaaaaagaa tcagaggtta ctatgacggc actgactcta ctgatgtcaa
      661 cagattgata actgaaattg atttgttact gcgtgaatac aagtatgccg ataaatgatg
      721 caaaaactac tcttcttttt tattctatct ccatttttaa gtttcagtca ggataatgta
      781 cacatacatt gcaaacgtat tatttcagat aattcgaaac ttcatagctt aaacaccgtt
      841 agcacaacag gtagtgattt tgatgtgaaa tacaatcgtt ttaactggac gataaatcct
      901 gattcagtat atatttccgg aaatgtattt acattgtttg aagtaaaagc accaatatcc
      961 aaattagaat ttgatttttc atctgcactt acaattgata gtattttata tcaatcaaca
     1021 ttattaccct atactagtaa cagtgacatt ataaccattt tacttcccgg aataatctca
     1081 acagggcaat tagatagtat tgaaattttt tatcatggtg taccacctac ttcaggtttt
     1141 gggtctttta atcagagtac tcacaatggt gtccctgtct tgtggacgct ttcagaacct
     1201 tttggcgcaa gcgactggtg gccatgtaaa aactcactta cagataaagt ggatagtata
     1261 gatgtttttg taactacaga tactgcctac aaagtagcat ccaatggaaa attaatttct
     1321 gaaattgctt caggcaatca aaaaataacg cattggaaaa gcaactaccc tactgcatcc
     1381 tatcttattg caattgcagt taccaattat gctgcatatt ccgactatgt gccaatggcc
     1441 ggtggagatt ctttgcaagt gctcaattat gtttatcctg aaaacttatc ttcagcacaa
     1501 acacaaacac ctgatataat tccaatcatt caactttatg acagcctcac tattccttat
     1561 cctttcagtc atgaaaagta tggtcatgca caatttaact ggggcggtgg catggaacat
     1621 caaaccatga gttttatggt taactttagt caggacctga tggcgcatga atgtgcacat
     1681 cagtggtttg gtgatttagt tacttgtggt agctgggaag atatttggct aaacgaaggt
     1741 tttgcaactt attttgaagg attaacaaag gaacattatt atccatcgtt gtggtataac
     1801 tggaaaaaac aaaagataaa caatataacc tcgcagcccg atggttcggt attttgtact
     1861 gacacaaata atgtcagcag aatatttaat ggacgcctaa cttataataa aggctcgtat
     1921 ttattgcaca tgcttcgctg gcaacttggc gactctgttt tttttaatgc attaacaagt
     1981 tatcttaatg acactttgtt gaaatatagt tttgcaaaga cacctgactt aatagcacat
     2041 ctgcaaaact caagtgggca gaatctgagt actttcttta atcaatggta ttacaatcag
     2101 ggttacccgt cttatcatat tacatggaat cagaataacc catctgtttg gctaaaaatt
     2161 gatcagacta catcacatcc ttcagtctca ttttttgaaa tgtctgtacc tgtcaggttt
     2221 tatggaaatg gtacagatac tactattgta tttaatcata ctttttcggg acaacaattt
     2281 actgcgtcaa tcccatggca ggtagattca gttgtgtttg atcctgattt atggatttta
     2341 tcagcaaaca atcttgttac aacaggaatt gaaacaaaag atttgagtaa cgaaaatcta
     2401 caaatttacc ctaaccctgt gcgtaatttc atcaatgtat tatgcaaaac aaattgtaaa
     2461 tacatcatta ctgatgccag tggcaaaaca atttcaaaag gagaattaca tcaaaaaaac
     2521 aaccggataa tattgaattc aaaaccgggt atttattttt taaatacatc agacgaaaac
     2581 agatggcgga gtcaatcatt cattattcaa tagcaaacat taaaggtcat gcggattaaa
     2641 acaatattaa tcacacttct tgcaatagta aatctgcgtg ctaatgctgc acagattctt
     2701 attccaatgg atgatacaca aaccaatcac ctaaaagctt atggtatcgc ttattgggtt
     2761 ttacaaagcg gagtagaaat taactggatg ttgaattaca gaggtggaag ttttatgttt
     2821 attcagaatc cgaaatttga aagtgagtta aaactgcgag gtgttagtta caatgtcatt
     2881 tcagaaggtc aggccaatca gattgtttct gaaatattag accctcaagt taatcaggac
     2941 gttgttaaat tagaaaaagt tcctaaaatt tgtgtttatt ctcctaaaga caaacgtcct
     3001 tgggatgatg cagtgacgct tgctttgacc tatgctgaaa ttccttatga tgttgtttat
     3061 gatgaagaga ttgttcaggg caaattagca ttgtatgact ggatgcactt acatcacgaa
     3121 gattttaccg gacagtatgg aaaattctat aatatgtata gaaatgcaac atggtatcag
     3181 gagcagcaaa gaagtatgga aagcatggcc aaacaacttg gatttgctaa agtgtcacaa
     3241 atgaaacttg ccgttgcaaa gaaaataaaa gagtttgttg caggtggtgg attcttgttt
     3301 actatgtgtt ccggtaccga cacgtatgac attgcgcttt cagcagaagg tttagatatt
     3361 tgcgaaagca tgtatgatgg tgacggcact tctccaaatt ataacgacca tcttgatttt
     3421 tcaaaaacat ttgcatttcg gaattatact ttaagtgtta atccacttga atatcgtttt
     3481 tcgaatattg atgtcacaac aacaagaaga gtaccgcgcg atgaagatgt gtttacttta
     3541 tttgattttt cagctaagtg ggaccctgtt cctactatgc tttgtcagga ccacatgcag
     3601 gtgattaaag gttttatggg tcagacaact gcattcaaca aaaattatat aaagcctgat
     3661 gttatcatta tgggtgaaaa taaagctgct aatgaagcta aatacataca tggagatttt
     3721 ggcaagggca cttggacatt ttacggaggt cacgacccgg aagactatca gcacatggtt
     3781 ggcgacccac ctacagactt gaatttacat ccgaacagtc cgggctaccg actgattctg
     3841 aacaatgttt tatttcctgc tgctaagaag aaaaagcaaa aaacttaaaa cgatttaagg
     3901 aagttattca ggtgttgatt aaatgcatct ttattttcaa gatggcctaa gtggccggaa
     3961 ttttcaatta tatgtaaaag actgccggaa atttgctgct gcatgaattc tgatttttca
     4021 ggaggtgtaa gcacatcatg ttcgccacaa agaattaata caggaaattt aattgaagaa
     4081 agattgctac atgactcttc tctttctgcc attgcttcca acgtttgcat caacacttca
     4141 ttatcagcct tcatcataag gtctaatgtt tgttttatta tttcattgtt tttttgaaat
     4201 gtgacaggtg aaaacaaaat cttacacata tcttcaagat atttttgctt tccaccattt
     4261 ctgattaatt caatattctc ataacgtttt gctttcccta ttgcattatc ggcatagcat
     4321 tgtgtatcgt ataaaacaag tccttttatt ctttcaggat atcgtttgac taaatttagt
     4381 gcaatgtatc cacccataga taagccacag acaataaact tactcacttg taatgaatca
     4441 agcaaaaaaa ttatatcatc ggcaaataaa tcaatagaaa atctttcttt cccttttgag
     4501 ctgcttccat atcctctaac gtcataggta attatgcgag catgatctga taaagcaatt
     4561 acctgctctt tccagattga ctgatcaaat ggatatccat ggattaacac cagcacctca
     4621 gttccggaag atgtatcgga atagtttaat acttctccgt caggctttat taatgccttt
     4681 gaatacatca tagtaacagg ttgttttctt gttatcatca ggaaaaatta cttcattcag
     4741 tgcctttgta aattttggcc aacttatctt tataattttc gagaataatt tttctgcgca
     4801 agctaagctt tggtgtcatt tcattgcgtt ctatactaaa ttcacgatcg agcaactcaa
     4861 atcgtttaat cgtttcataa tgacctagcg ttttattcac tacttctact tcttctgcaa
     4921 tgcgtttctt tacttcacta ctggccgcaa tctctgcatt ggattttact gaaatatttt
     4981 tctttgctgc ccagtctctg ataaatgcaa aattcggaac aataaatgct gatgcaaatt
     5041 tttgattttc gccaattacc attgcctgtt caataaatct tgattccttt aatttatttt
     5101 ctatcatctg tggcacgata tattttcctc ctgatgtttt aaagatttct ttcttgcgat
     5161 ctgtaatttt caaaaacttt ccatcttcaa aaacgccaat atcaccggta tggaaccagc
     5221 catcagcgtc tattacctct gctgtcaact caggactatt gtaataaccg agcattacat
     5281 tagggccttt gcataaaatt tcaccatcag aagcaatctt cacttcaaca ttttcgagta
     5341 cagttcctac agttccgaat ttaatgctgt cgggctgaaa attatttact gctataacag
     5401 gacttgtttc agtcagtccg taaccttcaa gcacaggaat ttttgcagca tggaatactc
     5461 gtgccaagcg tggttgcagt gctgcaccac ccgaagctat tgcctgaata tttccaccca
     5521 atgcctcacg ccatttactg aaaataagct tatttgccaa gcgcaactga aactcatacc
     5581 accatccatt agcacctttc aattcataac gcaaacccag attcaatgcc cagaaaaaca
     5641 aagctttttt tatacctttt aactctgctc cctttgaaac tattttatca taaactttct
     5701 caagcaatcg cggaaccgtt acaaaaatct gaggtttaat ttcttttaaa ttgtcggcaa
     5761 tagtatctaa actttcagca taataaattg aaacaccgaa ataaacatag agataagaaa
     5821 gcattctttc atagacatgg ttaagtggta aaaaacttaa tgccttccat gactcatgaa
     5881 acgggcacag cacatcacaa aatttaaagt tgcttacaag attattatgc gacaacatta
     5941 caccctttgg atttcctgta gttccggaag tgtaaataat cgttgccaaa tcatcaggct
     6001 ttatactatc tcttatctgc ttaacctctt cttgttttgg atgcttaagg ccaagatcaa
     6061 tcaattcgct ccaatgtggt aaatcttgat agcgatcgaa taaatatatc ttctctagaa
     6121 caggaagttc gctcttaatt tgactaacct tcttatataa atcaacatta gaaacaaata
     6181 aataacgcac tgttgactgg ttcaatgtga attttaaatc attttcacta atagtaggat
     6241 aaagcggaac atcagcaaca gcaatctgca agcaagcatg atcaacaaaa ttccattccg
     6301 gtctgttatt ggcaataatt ccgattttat ctccgggcct tattcctgaa tgtaataatc
     6361 cgtaactcag attatttatt gtttcaatta tatcggctgt tgcatatttt ttccatacac
     6421 catcctgttt tcctgccaaa gcatctgctt taggaaaacg tgaaagctga tatggaaaca
     6481 aatctataag tcgtgttact tccattcttc aattagtttt attgtgctgc ataaaaatat
     6541 aaaaagtatt taaatgtttt ttactattgc aatatgaact ataatgattt attaaacaaa
     6601 tcaaagccat tattgatatt tgatggcagt tgtgcttttt gcagatcatg tattcggttc
     6661 gttaaaaaat atgacatcaa acttcaattt gactttgcag acaacaatca gaataccacc
     6721 aaacttattt tagctcaata ctttataaat cctgaatctt taaactccgt tgttttgata
     6781 gaatacaaca aactacacat acagagtgat gcaattataa aagccttact tcttcttggt
     6841 gggagatgga aaattttagc tcgaataatg atggttttcc caaactcctt cagaaataaa
     6901 gtttacaata ttattgcagc caatagaaat aaattaagaa ttcattgcag agtatgaaaa
     6961 ccaattttac cgagcaaacc ggcatagcct atcctattat aatggcaccc atgtttttgg
     7021 taagcaatga ggctatgatg caatctgcaa tacgaacagg gattatgggt gtgtttccaa
     7081 ctttaaacta cagaaaagaa ggtgaacttg atgcattagt taaacggtta catcaattca
     7141 aggctaaaaa gcaaggtagt ttcggtgtca atttaattgt acaaaaaacc aaccctcttt
     7201 acaataggca tctaaaaata tgtgttgaaa acaaagtgcc tttttacatt acttcgttag
     7261 ggagcccacg cgaagttatt gaagccgcac attcttatgg cgcaaaagtt tattgtgatg
     7321 taaccaatat tgcacatgca gagaaatgcg ctaaacttgg ttgtgatggc tttattgccg
     7381 ttggtcaggg tgcaggcggt catgcaggcc cttatgctca aacggtattg attccaactt
     7441 tacgaaaaca ctttccttct atacctgtaa ttgcagcagg aggtattgcc accggtgacg
     7501 gcattgctgc aatgatggct ttaggtgcta caggagtaag tataggaaca agatatattg
     7561 catctacaga agcagaagtc agtgatgaat ataaaaacag tattttaaat tcaggtatgg
     7621 ataatattgt tatgacagaa aggctttccg gaacaccatg caacatcatc aatacacctt
     7681 atgcaaaaaa aattggctat aaacaaaatt ggttagaaaa gtggctgagc aataactcca
     7741 caacaaaaaa atattttaaa atgatggtgc agtacaaagg cgttaaagca cttgaacaag
     7801 caattaaacc aggtaattac aaaacattat ggactgcagg gcaaactgtt gaacttattg
     7861 atgatataat aaactgtgat gaaattacag agcggttaat taaagaaacc catctgtc
//
LOCUS       DAORQT010000057         7905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1342_length_7905_cov_7.350318, whole genome shotgun sequence.
ACCESSION   DAORQT010000057 DAORQT010000000
VERSION     DAORQT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1342_length_7905_cov_7.350318"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..361
                     /locus_tag="PK134_03365"
                     /pseudo
     CDS             47..361
                     /locus_tag="PK134_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
     gene            368..1966
                     /locus_tag="PK134_03370"
     CDS             368..1966
                     /locus_tag="PK134_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRB37918.1"
                     /translation="MRFVFAIIPILWLNIAIAQQSKKPVKKIVLGKDQVSEMKKAGSE
                     YFKSENYKTALPIYQQLQENDPNNAEFNYRLGLCYLNTNVNKKLAAGFLIKAAEGKDV
                     PKDVYYYTGRALLILEEIDDAVDAYEKFKIAHGGKVSPKLNLENNVEWCYHFRDMKKT
                     PIEVSFTNLGKLVNSPNNDYRPVCDMLGNVVYFSSNRKSNTGAIVDGYGEVISDGYFT
                     KKDTGYSKAKNLGPNINTMYYEEPLQLNSNSDKLLVFKEGGSISSPLYTSELNGKSFE
                     KAVAINKFPASKVDGACMTDDGKTIYFSADLKGGKGGKDIWMTTLNSKNEWSNPVNLG
                     DAVNTKYDEVNPWLFFDNQTLFFASEGHNSIGGFDIFFSTKPHESMDWSAAKNVGYPL
                     NTTDDNKYFSLTADCKTGFVSQTTEHGSGELDIVKFVCTNPIIKQEKVVCDFTFLKAD
                     GTFARDANCMIIRNDTGESSGVKTINGSNGKLYVYLGPGNYKVKVKGVKSGRADEDFI
                     ITGNEPKMQLVKTFKLTPPVKEGK"
     gene            1968..2738
                     /locus_tag="PK134_03375"
     CDS             1968..2738
                     /locus_tag="PK134_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRB37919.1"
                     /translation="MNLKLHRPLAFFDLETTGVVIGMDRIVELGILKILPDGTKTNKT
                     WRINPEMHIPEVVSKIHGIYDKDVADCPTFKEVANDVNHYLNNVDLAGYNSNRFDVPL
                     LVDEFLRAGIEFDIRGRKLIDVQHIFHKMEQRTLSAAYKFYCNQELIDAHSAEADVRA
                     TYEVLEAQLERYSDQLQPDAAFLHAFCNNSKNVDLAGRIVYDDKKREVFNFGKYKGRL
                     VTDVFKTDPSYYDWMLKGDFSRDTKQVVTALRLREREN"
     gene            2809..5067
                     /locus_tag="PK134_03380"
     CDS             2809..5067
                     /locus_tag="PK134_03380"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRB37920.1"
                     /translation="MLMGSDNNSDKNNLPSVSASGKGSKVVIDLEAEKKEISKRYRHL
                     LRACKSDLDKKDKKLIRLAFDTALEAHKEMRRKSGEPYIYHPIAVAQIVAEEIGLRSA
                     IPVVCALLHDTVEDTYLTLEDIRDLFGPVAEKIIDGLTKIAGVFDHSSSLQAENFRKM
                     LLTLSVDVRVILIKLADRLHNMRTLGSMARDKQLKIASETAYLYAPLAHRLGLYNVKT
                     ELDDLALKYTEPEVYKTLAEKLRDSKKERDKFISEFIHPIKDELNKQGFKFEIKGRPK
                     SIHSIWEKMKKQGVTFEEIYDLFAIRIIIDSPQNQEKADCWKVYSIVTDSYRPNPDRL
                     RDWISTPKSNGYEALHTTVMSDQGKWVEVQIRSQRMNETAEKGFAAHWKYKESSQESA
                     LDEWLTRIREMLENPDSNALDFVDDFKLNLFADEIFVFTPKGELKTLPVNSTALDFAY
                     EIHTDIGSRCIGAKINHRLVPISHKLNSGDQVEILTSDKQRPKEDWLSFVITAKAKGR
                     IKSSLKDEKRILGEQGKEIFDRKIKHLKIPVAEVNMNDILLYYKLPNVLELYYRIAMD
                     AVDLKQLREAVNYKDQRVRSQRTEPTLEELVTQARGSSDMLVIGENLNKIDYKLSPCC
                     NPIPGDDVFGFITINDGIKIHRVNCPNAMQLMSNYAYRIVKAKWTGQEQLSFLAGIKI
                     TGMDDVGVVNNITKVISSELKVNMRSISIESNEGLFEGTIMLFVHDTEHLTKLMKKLS
                     ALSGILNVTRIT"
     gene            5070..5570
                     /locus_tag="PK134_03385"
     CDS             5070..5570
                     /locus_tag="PK134_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37921.1"
                     /translation="MYVSFDQLPDDSRIWIFQSDRLLSEEEIESINPILNFFISDWTA
                     HQQTLRASFTFMHNHFLIIGVDENMAIASGCSLDKVFHVVRACGQKINTDFLNRLHIA
                     AMIQNVITLHSIKQILDIAENRNNNEPLMIFSNIIETKSEMKNNWLIPIEKSWLAPKL
                     KSGSKI"
     gene            5587..5937
                     /locus_tag="PK134_03390"
     CDS             5587..5937
                     /locus_tag="PK134_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRB37922.1"
                     /translation="MNFIIKLIISTLAVMMVSYILPGVHVQSFGIALMVACVLAFLNA
                     LIKPILVLFTLPITLITLGLFLLVINAIIIIITDRLIDGFEVSSFGIALLFSLILSIV
                     TSVMESLAGQNQRQ"
     gene            complement(5986..6792)
                     /locus_tag="PK134_03395"
     CDS             complement(5986..6792)
                     /locus_tag="PK134_03395"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB37923.1"
                     /translation="MLKSISLVAVLAVFASGAFALNPSKEYKVLPDKYGMTYKEEKIK
                     TPDGAILNAWMFELPKKSTNWIIMSGSGDGNMADNIEIANSFLSAGYNVAMYDYRGYG
                     SSSDFKIDPDLFIYPQFIVDLNAVCDYLRKSRAITKFNLYGKAIGAGLSIGVGANRTE
                     TQKVIADGPWIGLEVMKKKIKDKTGKEVVMPFGFDKNHEPMYAMEKPKGIKGLMVIVS
                     SQDNLINPTDVKQIKGVSETYVVKNSPSNAENFTIDKNVYFEKISKFLGN"
     gene            complement(6947..7768)
                     /locus_tag="PK134_03400"
     CDS             complement(6947..7768)
                     /locus_tag="PK134_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRB37924.1"
                     /translation="MSQDTSFTVYGHRGCRGLMPENTIPAFLKAIELNVLHLELDVVV
                     SADSQVVVSHEPWINNENCLMPDSTPLPDFKRAAYSIYKMPYEQVKGYDCGSKFYAKF
                     PGQHKMVVGKPLLSEVINTADTFCCNTKGVLPYYSIEIKSKKSWIGIFQPETKQLVDL
                     VMKVVQQYGEKERFVLQSFDPEVLRILHDDYPEYTLAALNETNRDVAAFFNELGFVTT
                     IYSPWYVLATAKAIAYCKQQEIKIIPWTVNDDKVFLKLKYSGVNGIITDYPDRYR"
BASE COUNT         2676 a         1386 c         1432 g         2411 t
ORIGIN      
        1 ccggcctgcg taccaacatc gaaacgttaa actcaactat tggtgagcgc gaaaaaacaa
       61 actttcgcct gatgaaattg caggtggaac ttttacattg accaatgtag gcacttttgg
      121 aaatgttatg ggtacaccca ttatcaatca accacaagta gctattatgg ctgcaggtgc
      181 cattcgtaaa aaacctgctg taattgaaac tgaatttggc gatactatag gtatccgtca
      241 catgatgttt ctctctttat cttatgacca tagaatagtt gatggaagtc ttggaggtag
      301 ttttctccgt aaggtagcag actatcttga gcagtttgat gttaacagaa atttattata
      361 atttgttatg agatttgtat ttgccattat tccaatactc tggctgaata ttgcaatagc
      421 gcaacagtca aagaaacctg taaaaaaaat tgttttagga aaagatcagg tttccgaaat
      481 gaaaaaagca ggttctgaat atttcaagag tgagaattat aagactgcat tacctattta
      541 tcagcaactt caggaaaacg atccaaacaa tgctgagttt aattacagac ttggtctttg
      601 ctatttaaat accaatgtaa ataaaaaatt agcggcaggt tttctgataa aagccgctga
      661 aggaaaagat gttccaaaag atgtttatta ctatactggt cgggcacttc tcattcttga
      721 agaaatagat gatgctgtag atgcttatga gaaatttaaa atagctcatg gtggaaaagt
      781 tagcccaaaa ctgaatctcg aaaacaatgt ggaatggtgc tatcactttc gcgatatgaa
      841 gaaaacacca attgaagtaa gttttactaa tctgggtaaa ttggttaatt cacctaacaa
      901 cgattacaga ccggtttgtg atatgttagg taatgtagtt tatttcagct caaatagaaa
      961 aagcaacaca ggtgccattg ttgatggtta cggagaagtt atttcagacg gttattttac
     1021 taaaaaagat acaggctaca gtaaagcaaa aaatcttgga ccaaacatta acaccatgta
     1081 ttatgaggaa ccattgcaat taaattccaa cagcgacaaa ttgttggttt ttaaagaagg
     1141 tggcagcatt tcttctccat tatatacatc agaactcaat ggaaaatcat ttgagaaagc
     1201 agttgctata aacaaatttc ctgcttcaaa agttgatggt gcctgcatga cagatgatgg
     1261 aaaaacaatc tatttttctg cagaccttaa aggtggtaaa ggtggtaaag atatttggat
     1321 gacaacttta aacagtaaaa acgaatggtc taatccggtg aatcttggtg atgctgtaaa
     1381 tacaaaatat gatgaagtaa acccttggtt gttttttgat aatcagactt tgttttttgc
     1441 ttccgaaggc cataatagta ttggcggatt tgatattttc ttcagtacaa agccgcatga
     1501 atctatggac tggagtgctg ctaaaaatgt aggctatcca ttaaatacta ctgacgataa
     1561 taaatatttt tctttaacag ctgattgtaa aaccggcttt gtaagtcaga ctactgaaca
     1621 tggttccgga gagttggata ttgtaaaatt tgtttgcacc aacccaatca ttaaacaaga
     1681 aaaagttgta tgtgatttta cttttttaaa agctgatggt acttttgcac gtgatgccaa
     1741 ctgtatgatt ataagaaacg atacaggtga atcatcaggt gtgaaaacaa ttaatggctc
     1801 aaacggcaaa ctttatgttt acctcggccc gggtaattat aaagttaaag taaaaggcgt
     1861 aaagtcaggt cgtgctgatg aggattttat catcacaggc aatgaaccta aaatgcaatt
     1921 ggtaaaaact tttaaattga cacctcccgt taaagaagga aaataaaatg aatctgaaac
     1981 tgcacagacc acttgctttt tttgatcttg aaactacagg cgtagttata ggcatggaca
     2041 gaattgttga attggggatt ttaaaaattc tacctgatgg aacaaagaca aacaaaacat
     2101 ggcgtataaa tcctgaaatg cacattcctg aagtggtatc aaaaattcat ggcatttatg
     2161 ataaagatgt ggccgactgc cctactttta aagaagtggc aaacgatgta aatcactatc
     2221 tcaataatgt tgaccttgcc ggatacaatt caaaccgatt tgatgtaccc ttgctggttg
     2281 atgaattttt aagagcaggt attgaatttg acatacgagg acgaaagtta attgatgtac
     2341 aacacatctt tcataaaatg gaacaacgca cattgagtgc tgcatataaa ttctactgca
     2401 atcaggagtt aatagatgcc cattctgccg aagcagatgt gagagctacc tatgaagttc
     2461 tggaagcgca attagaacgt tatagcgatc agctacaacc tgacgcagcc tttcttcacg
     2521 ccttttgtaa taacagtaag aatgttgatt tggccgggcg tatagtttat gatgacaaaa
     2581 agcgcgaagt atttaatttt ggaaagtaca aaggacgctt ggtgactgac gtttttaaaa
     2641 ctgatcctag ctattatgac tggatgctta aaggcgattt ttcgagagat actaaacaag
     2701 ttgtaacggc attgcgatta cgcgaaagag aaaactaaat atatgtttga cctgtttgat
     2761 tatttctgct gattaaaatt tgcaggtaat ataaaaatct gtacttttat gctgatgggt
     2821 tccgacaata attcagataa aaataatctt ccttctgttt ctgcttcagg taaaggcagc
     2881 aaagttgtta ttgaccttga agctgagaaa aaagaaatca gtaaacgcta taggcattta
     2941 cttagagcat gcaaatcaga tttagataaa aaagataaaa aacttatcag gcttgcattt
     3001 gatactgctc ttgaagctca taaggaaatg cgcaggaaat caggagagcc atatatctat
     3061 cacccgattg ccgttgcaca gattgttgct gaagaaatcg gacttcgttc tgccatccct
     3121 gtagtttgcg ctttactgca tgacactgtt gaagatactt atcttacatt agaagatata
     3181 cgtgatttgt ttggccctgt tgcagaaaaa attattgatg ggctgacaaa aattgcagga
     3241 gtttttgatc attcaagctc tttgcaggca gagaatttca gaaagatgtt acttacatta
     3301 agtgttgacg tcagagtaat tctcatcaaa cttgccgaca gacttcacaa catgcgtaca
     3361 cttggtagta tggcacgtga caagcagttg aaaatagctt ctgaaacagc atacctatat
     3421 gcacctttgg cacatcgtct tgggctttat aatgtaaaaa cagaacttga cgatctggca
     3481 ttgaaatata cagaacccga agtatataaa acacttgccg aaaaactaag agactcaaaa
     3541 aaagaacgcg acaaatttat ttcggaattt attcatccga taaaagatga gttaaataaa
     3601 caaggtttca aatttgagat taaaggaagg ccaaaatcta ttcattccat ctgggagaag
     3661 atgaaaaagc aaggagtaac ctttgaagaa atttatgacc tatttgcaat aagaataatt
     3721 attgactcac cacaaaatca ggaaaaagct gattgctgga aagtgtattc tattgttact
     3781 gatagttacc gaccaaaccc tgacaggctg cgcgattgga tatcaacacc aaaatcgaat
     3841 ggctatgaag cattgcacac aacagtgatg agtgatcagg gcaaatgggt tgaggtacaa
     3901 attcgttcac aaagaatgaa tgaaactgct gaaaaaggtt ttgcagcaca ttggaaatat
     3961 aaagagtctt cgcaagagtc ggccttggat gaatggctta cacgtatcag agaaatgctt
     4021 gaaaaccccg acagtaatgc tcttgatttt gttgatgact ttaagcttaa tctctttgca
     4081 gacgaaattt ttgtttttac acctaaaggt gaattaaaaa ctttacccgt caacagtact
     4141 gcattagact ttgcttatga aatacacacc gatataggca gcagatgtat cggagcaaaa
     4201 attaatcatc gtttagtacc catttcacat aaactgaata gtggtgatca ggttgaaatt
     4261 ttaacctccg acaaacaacg accaaaagaa gactggttaa gttttgtgat tactgcaaaa
     4321 gctaaaggca gaataaaatc atcattaaaa gatgaaaaga gaattcttgg tgaacaaggc
     4381 aaggaaatat ttgaccgtaa aataaaacat cttaaaattc ctgtagcaga agttaacatg
     4441 aatgatatac ttctctatta caaacttccg aatgttttgg agttgtatta tcgtatagcc
     4501 atggatgctg ttgatctgaa acagttgcgc gaagccgtta attataaaga ccaacgtgtg
     4561 cgcagtcagc gtacagaacc tactttagaa gagttggtaa cacaggcacg aggctcgtcc
     4621 gatatgcttg ttattggtga aaacctgaat aaaatagatt ataaactttc accatgctgt
     4681 aatcctattc ctggtgatga cgtttttggt ttcatcacca ttaatgacgg aataaaaata
     4741 catcgtgtaa actgccccaa tgccatgcaa ctgatgagca actatgctta tagaattgta
     4801 aaagcaaaat ggaccggtca ggagcagctt tctttcttgg caggtataaa gattacagga
     4861 atggatgatg taggtgttgt aaataatatt accaaggtta tttcaagcga actgaaagtg
     4921 aatatgcgtt ctatctcaat cgaaagtaat gaaggtttgt ttgaaggaac catcatgctt
     4981 tttgttcacg acaccgaaca tctgactaaa ctgatgaaaa agctttctgc acttagcggt
     5041 attttaaatg taactcgaat tacttaatca tgtacgtatc ttttgaccaa ttaccggacg
     5101 actcccgtat ctggattttt cagtcggata gattattatc agaagaagaa attgaatcta
     5161 tcaatccaat acttaatttt tttatcagtg actggacagc acatcaacaa actctaagag
     5221 cctcattcac ttttatgcat aatcattttc tgattattgg agttgatgaa aatatggcta
     5281 ttgccagcgg ctgttctctc gataaggttt ttcatgttgt tcgtgcatgt ggccaaaaaa
     5341 ttaatactga ctttttaaac cgtctgcaca ttgcagcaat gattcaaaat gttattacgc
     5401 ttcactctat aaaacaaata ttggatattg cagaaaacag aaacaataat gagccattaa
     5461 tgatattcag caacatcatt gaaaccaaaa gtgaaatgaa aaataactgg ctaataccaa
     5521 ttgaaaaatc atggcttgca ccaaagttaa aatcaggaag taaaatttaa ttattaaaac
     5581 tattttatga attttataat caaactcatt atctctactc tggcagttat gatggtgtca
     5641 tacattctgc ccggtgtgca tgttcaaagt tttggcattg cacttatggt agcatgtgtc
     5701 ttagcatttt taaatgcatt gataaaacca atacttgtac ttttcacact acctattaca
     5761 ctcatcacat taggtttatt tttgctggtc attaatgcaa tcatcattat cataaccgac
     5821 aggctaattg atggatttga agtaagcagt tttggtattg cacttctgtt ctctttgata
     5881 ttaagcattg ttacttctgt aatggagtcg cttgccggac aaaatcaaag acaataaaaa
     5941 accggcaact ttaaaagtgc cggcttatgt gctgtcaaat aaacattagt ttcctaaaaa
     6001 ctttgaaatc ttttcaaaat aaacattttt atcgattgta aaattttctg cattggatgg
     6061 agaatttttt acaacatagg tttcactgac acctttaatt tgttttacat ctgttggatt
     6121 tatcagatta tcctgagatg aaacaataac cattaatccc ttaatgcctt taggtttttc
     6181 catagcatac attggctcat gatttttgtc aaagccaaaa ggcattacca cctctttacc
     6241 cgttttatct tttatttttt tcttcattac ttcaaggcca atccatggac catcggcaat
     6301 aactttctgt gtttctgttc tgtttgcacc aacaccaatt gaaagtcctg cgccaatagc
     6361 tttaccatat aaattaaact ttgtgatggc gcgcgactta cgtaagtaat cacaaacagc
     6421 attaaggtca acaataaact gaggatagat aaataaatcg gggtcaattt taaaatcgct
     6481 gctgctgcca taaccacgat aatcatacat ggctacatta tatcctgcac ttaaaaatga
     6541 attagcaatt tcaatattgt cggccatatt gccatcacca ctgccactca taattatcca
     6601 gttggtagat tttttcggca actcaaacat ccatgcattg agaattgcac catcaggagt
     6661 tttaattttt tcttctttat aagtcattcc gtacttatca ggaagtacct tatattcctt
     6721 tgaaggattt agtgcaaaag cacctgaagc aaataccgcc aatacggcaa ctaatgaaat
     6781 tgatttaagc atcgtaattt gattgttaaa tttaagcttc aaaaataagt acactatcca
     6841 tgaataagta attaattttt taacaattct tgccatttca ttgctgataa aatctacttt
     6901 gcggcaaaaa aacaaatatc tgcaattatt tctcacttag aataacttaa cgatagcgat
     6961 cgggataatc tgtaataatg ccattaacac cgctgtattt cagctttaaa aatactttgt
     7021 catcattcac cgtccaggga ataattttaa tttcttgctg tttgcaatat gctattgcct
     7081 ttgcagttgc caatacatac caagggctgt aaattgttgt cacaaaacca agttcattaa
     7141 aaaaagccgc cacgtcccta ttcgtttcat tcaaagctgc caaagtatat tcaggataat
     7201 catcatgcaa aatacgcaac acttcaggat cgaatgactg caatacaaat ctttctttct
     7261 cgccatactg ttgcacaacc ttcatcacca aatcaactaa ctgctttgtt tcaggctgaa
     7321 atatgcctat ccaactcttc ttacttttta tttcaatgga ataatatggc aacactcctt
     7381 ttgtattaca acaaaaagta tcagcagtgt taattacttc agaaagcaaa ggtttaccta
     7441 caaccatttt atgttgtccg ggaaactttg cataaaactt actaccgcaa tcataacctt
     7501 ttacctgttc ataaggcatc ttgtaaatac tgtaagctgc tcttttaaaa tccggcaatg
     7561 gtgtactgtc aggcatcagg cagttttcgt tatttatcca gggctcatgc gaaaccacta
     7621 cctgagaatc tgccgataca acaacatcta attcaagatg caaaacattt agctcaatag
     7681 ctttcagaaa tgcaggaatt gtattttcag gcattaaccc acgacatcca cgatgcccat
     7741 aaactgtgaa agatgtgtct tgtgacatga gatggatact aaataaagaa aaaagaaaaa
     7801 ttaaaaatag aataatacgc acaataacag ttcattatta actaaaaata aaaacgaggc
     7861 aatcttgaga gtgccccgta gtagatgttt tcacaacgga ataat
//
LOCUS       DAORQT010000058         7903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1344_length_7903_cov_7.534659, whole genome shotgun sequence.
ACCESSION   DAORQT010000058 DAORQT010000000
VERSION     DAORQT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1344_length_7903_cov_7.534659"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK134_03405"
     CDS             complement(<1..182)
                     /locus_tag="PK134_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRB37925.1"
                     /translation="MGIYLIVIVFMLVGWLVSAQLKRKFEQYSHETLGTGLSGQEIAE
                     KMLHDNGINDVKVISV"
     gene            complement(306..1397)
                     /locus_tag="PK134_03410"
     CDS             complement(306..1397)
                     /locus_tag="PK134_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37926.1"
                     /translation="MSYSQTKAMLAIAKASFRSITRSPSAVVFTLVFPLTFILVFGFL
                     SGTGLKVKLFLTPSSDHHNPIYEATKNIDVINWIEGDSAIASKKLQQGKIDGILVITK
                     NITEGKIDYTARLITSTAAPEGGALSKNILDNLISKINLYDAHVAHPLAQLKNEEIAG
                     REYKKIDFILPGQLGFALLSTGVFGTAFVFFSLRQTLVIKRFFATPVKRIYIVLGETL
                     SRMVFSLLGACFIIGVGRFAFGFTLINGISTFLTMMLICAFGLIVFMGFGFVVSGIAK
                     SESTIPPIANIITMPQFLLSGTFFPIDVFPKWLQPFCKALPLTYLNDALREVAFEGAG
                     LIDVISQLMVLLLWGVIIYIVAAKTFRWE"
     gene            1498..2049
                     /locus_tag="PK134_03415"
     CDS             1498..2049
                     /locus_tag="PK134_03415"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HRB37927.1"
                     /translation="MNHTFSLFQSMERDKIILSFKGDITQDLLASVYQIMESRMDQDK
                     TDSKRKKRFYHILVECLQNVYHHMEDFRNVHELDEISDGSAIFIIAKAKGEGYRIITG
                     NYIKKDESEDLKSKLEKINSMNTDELRTYYLETLSTTELSQKGGAGLGMIDIARKSGH
                     KIVYSFDGVSDTVNFFTLEVTIN"
     gene            2060..2443
                     /locus_tag="PK134_03420"
     CDS             2060..2443
                     /locus_tag="PK134_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRB37928.1"
                     /translation="MDKLIIEPGIKTPHIVFDAANGSLEIKGKSIPENSIEFYKPVYD
                     WLDTYTTQPKDQTLLTVQLEYFNTSSSKCLLDLFRKLENIHSSGKGKVNVKWLYEEDD
                     EDIMEAGDDYQTIVKLPFSIEKISG"
     gene            complement(2444..>3971)
                     /locus_tag="PK134_03425"
     CDS             complement(2444..>3971)
                     /locus_tag="PK134_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRB37929.1"
                     /translation="ELAIPITANTKEVTIYYETSPDAQALQWLNPEQTAGKKHPFLYT
                     QSEAILARSWIPLQDTPGIKFTWTATIHCPKELMALMSCPQNPKQIGNGTYSFKMPFA
                     VPSYLMALSIGDLAYKAYDNRSGIYAEPVMLAKAFHEFTDLPAMISSAEQLYGKYPWL
                     QYDVLVLPPSFPFGGMENPCLTFATPTIITGDRSLVSLIAHELAHSWSGNLVTNATWN
                     DFWLNEGFTVYFESRIMEKIYGKDYADMLNLLSQNELKHTVADMGVNNPDTKLFLDLK
                     GRDPDDGMGDIAYEKGRFFLMALEEAAGRDMWDAFLKKYFEEHRFQTMTTKEFLHYLE
                     VNLLQENPLIKQKVNVDEWVYGTGLPKSLPVIKSKYFDQVEKAAAAFIKQPTVMPDTA
                     GWTTHHYLHFLRTIAPEMTYEKAVALDNHFHFTQIQNSEIAFDWFMIAFACHYKPAYK
                     ALENFLNTVGRRKFIAPLYRKAAADATLRPWIEQIYKSARPGYHAVAVKTIDEIFEGK
                     "
     assembly_gap    3972..4044
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4045..4318)
                     /locus_tag="PK134_03430"
     CDS             complement(<4045..4318)
                     /locus_tag="PK134_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRB37930.1"
                     /translation="MKNILFLILIPFTMFAKKPEDERGIYVKDQHSYSNPAEIVVQHL
                     DLSLKADFSTKKLVGFVTLQVKNLTNADTLRLDTYNLKIESVKINDA"
     gene            complement(4326..4775)
                     /locus_tag="PK134_03435"
     CDS             complement(4326..4775)
                     /locus_tag="PK134_03435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB37931.1"
                     /translation="MNIFWKFKSFNDLTAHELHDFLQLRQQVFIVEQNCPYGDIDKTD
                     KLSFHLMAMANQQVVGYARIIPPGVLYDEVSVGRIVTHQQLRRTGLGKLVVEKSLDEC
                     KSLFPNSAIKIMAQSYLRKFYESFGFVVISEEFLEDNIPHIYMIKTK"
     gene            4915..5943
                     /locus_tag="PK134_03440"
     CDS             4915..5943
                     /locus_tag="PK134_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRB37932.1"
                     /translation="MSLPQLIVTDSSRNGKLPFAPFKRTQEESAQQPKTEKVSNKVAK
                     QKLSLKTDSLKGNTTVVDTIKPIANDSIQLAIDSSVADMSRLFRFTDEENDELNNSDQ
                     QFISSSAYFKQQQLEVKSSSPQLFNKIVPDWFTIVLFFLIVGITTVKVFYQNIFRQLV
                     EAVYSFAVTNQVVRDENLLVQRASVLLNVIFYCSSGLFFYWISVRYNWNHPILNEGLF
                     RFFFFAFVTAVFFTVKMLLLKLIGTIFNLDKPVAVFIFSIFLTNNLLGVLLVLITSFM
                     AYLSGVGLINYIYLVLGILSLAFIFLLYRAFLISRTVQGYSLYYLILYFCTLEIVPIA
                     LIVKFASE"
     gene            5967..6722
                     /locus_tag="PK134_03445"
     CDS             5967..6722
                     /locus_tag="PK134_03445"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRB37933.1"
                     /translation="MAGNSKVKTILVTQPKPETEKNPYFDLAKKFNVKIDFRPFIQIE
                     GIPAKDFRKDRVNVAEFQAVILTSRQAADHYFRMAAEMRMTNFDEVKFFCVSESTAFY
                     LQKYITYRKRKVFYGNNSINDLTDVLKKHKKEKFLLPCSEVMNPDIINTLKDGDLNFI
                     TAAMYRTVSSDLSDVNIETFDMIVFFSPSGVQSLLKNFPKYKQGNTRIATFGVTTSQT
                     AKDAKLKVEVEAPVPQAPSMTMAIEQYLKKANK"
     gene            complement(6727..>7903)
                     /locus_tag="PK134_03450"
     CDS             complement(6727..>7903)
                     /locus_tag="PK134_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37934.1"
                     /translation="LSRKIEIFKTSDCVDKERLTKYVQGKMTDAEKYAVEKHLTDCEL
                     CSDAAEGLALLTSMKPLDETIKQVSDKYSSSAKQSPSINRWYYAAASVAILIATGWII
                     SNYSFKHENTAEQINAPAVQQKINTETPQTSSDTKLNESTIQPPPPPAEERIQQASKN
                     IIASHNAMVAKASKEDKAFAATEPVQKNEAIAEPQSADVAAPIVTESASAGAAVVDAV
                     VPVTKNIEGLKVYDYSREYKREKNVAKEDNGIPARYESRKDMEKSKTLNNMQSRTIGY
                     QDFLAEALVDYNNKKYSSAINKLQLLLSDNHSDVNALFYTAMSYSESQQYDKALHFLD
                     RLDAQSNNTFNQESAWHRALLLLQKGEQDKAKELLQKIISSKGFYATQAQQKLSEIK"
BASE COUNT         2434 a         1400 c         1452 g         2544 t
ORIGIN      
        1 tcaacagaaa taacttttac atcattaata ccgttgtcat gaagcatttt ttctgcaatc
       61 tcctgaccac taagacctgt tcctaaagtt tcatgcgagt attgttcaaa ctttcgtttt
      121 agctgggcgc ttactaacca cccgacaagc ataaatacta ttactataag atatattccc
      181 atttattgat ttttttaaat ttttaaactg cttttcacta atttttttta accacaatca
      241 aacagtgtgc aaatatacac aacctgcatt tttgtcagtg ttttttatga ttattattga
      301 atgaattact cccagcgaaa cgttttggca gctacaatgt aaataataac accccaaagc
      361 agcaatacca ttaactgtga aatgacatct attaatcctg caccttcaaa tgccacttcg
      421 cgtaaagcat cattaaggta ggttaacggc agtgccttgc aaaacggttg cagccatttt
      481 ggaaaaacat caatcggaaa aaatgttccc gacaacaaaa actgcggcat agtaatgatg
      541 ttggcaatgg gtggaattgt gctttcactt tttgcaattc cggaaaccac aaagccaaaa
      601 cccataaaaa cgataagtcc aaaggcgcag ataagcatca ttgtaagaaa agtgcttatg
      661 ccattaatga gtgtaaaccc aaatgcaaac ctgcccacac ctattatgaa gcaagcacca
      721 agcaatgaaa ataccattcg gcttaatgtt tcacccagaa caatgtaaat acgttttact
      781 ggcgtggcaa aaaaccgttt tatgaccaag gtttgtctga ggctgaaaaa aacaaatgca
      841 gttccgaaaa ctcctgttga caataaggca aaacctaact gaccgggaag tataaaatct
      901 attttcttgt attcgcgtcc tgcaatttct tcatttttta actgtgccaa tggatgtgct
      961 acatgtgcat cgtacaaatt tattttgctt ataagattat ctaaaatgtt tttagaaagt
     1021 gctccacctt caggtgcagc tgtggaggta ataagtctgg ccgtataatc tattttgcct
     1081 tcagtaatat tttttgtgat aaccaaaatc ccatcaatct ttccttgctg aagtttttta
     1141 cttgcaatag cagaatcgcc ttcaatccaa ttgataacgt caatgttttt tgtggcttca
     1201 taaatcggat tatgatggtc gcttgaaggg gttagaaata atttgacttt aagtcctgta
     1261 cccgataaaa atccaaaaac caaaataaag gtaagcggaa aaaccagagt aaaaactact
     1321 gcactggggc tgcgtgttat tgacctgaaa ctggctttag caatagccag catggccttt
     1381 gtttgagaat aactcatgtt gtttatgtca gaaatgttgc aaatgtagtt aagttggttt
     1441 ttatgaatta aaataaaaaa tgtacgtttg ccgtaataac ataggttcgt caattcaatg
     1501 aatcacacat tctcattatt tcagagcatg gagagggata agattatcct ttctttcaaa
     1561 ggagatatta cccaggattt gcttgcttct gtatatcaga ttatggagtc gagaatggat
     1621 caggataaga ctgattccaa gaggaagaaa agattttatc acattttagt tgaatgtctt
     1681 cagaatgtat atcaccacat ggaagatttc agaaatgtgc atgagctgga tgaaatttct
     1741 gatggctctg caatttttat cattgccaag gcaaaaggtg agggctacag aattattaca
     1801 ggaaactata tcaagaaaga tgaatcggaa gaccttaaat cgaagcttga gaaaattaat
     1861 tctatgaaca ccgatgaact aagaacttat tatcttgaaa cactaagcac tacagagctt
     1921 tcgcaaaaag gtggtgccgg tttaggaatg attgatattg cccgcaagtc gggacataaa
     1981 atagtttaca gttttgacgg tgtaagcgac actgttaatt tttttaccct tgaagtaaca
     2041 attaattaaa tcaaagaaaa tggataagct gattattgaa ccgggcataa aaacacctca
     2101 cattgtgttt gacgctgcca atggaagtct tgaaattaaa ggtaaatcca ttccggaaaa
     2161 ttctattgaa ttttataagc ctgtttacga ttggcttgat acttatacaa cacaacccaa
     2221 agatcaaacc ttgctgacag tgcagttaga atattttaac actagctctt ctaaatgttt
     2281 gctcgactta ttcaggaaac ttgaaaacat tcattcttca ggcaaaggaa aagtaaatgt
     2341 aaagtggctt tatgaggaag atgatgaaga cattatggaa gccggtgatg actatcagac
     2401 cattgtaaaa cttcctttca gtatcgagaa aatttcagga taattatttt ccttcaaaaa
     2461 tttcgtcaat agtttttaca gcaactgcat gataacccgg acgtgccgac ttataaattt
     2521 gttctatcca aggtcgtaat gttgcatctg ctgcagcttt tcgataaagt ggtgcaataa
     2581 attttctgcg tcctactgta ttcagaaaat tttctaatgc tttatatgca ggcttgtagt
     2641 gacaggcaaa ggctatcata aaccaatcaa atgcaatttc tgaattttga atctgagtga
     2701 aatgaaaatg attatctaat gcaacagctt tttcgtaggt catttcggga gcaatagttc
     2761 gtaaaaaatg caggtaatga tgcgttgtcc atcctgcagt atctggcata actgttggct
     2821 gtttgataaa tgctgctgct gctttctcaa cctgatcaaa atatttgctt ttaataacag
     2881 gcagactctt aggaagtccg gttccataaa cccattcatc aacatttact ttttgtttta
     2941 tcaacggatt ttcctgcaaa agatttacct ccagataatg tagaaattct tttgttgtca
     3001 tggtttgaaa gcgatgttct tcaaaatact tttttaaaaa tgcatcccac atatcacggc
     3061 cggctgcttc ttctaatgcc atcagaaaaa aacgcccttt ttcataggca atatcaccca
     3121 tgccatcatc aggatcacga cctttaaggt caagaaaaag tttagtatcc ggattgttaa
     3181 cacccatatc ggcaacggta tgtttcaatt cattttgtga aagtaagttc agcatgtcag
     3241 cataatcttt accataaatt ttttccataa tgcgcgattc aaagtaaact gtaaatcctt
     3301 catttagcca gaagtcattc catgttgcat tggtaactaa attgccactc cacgaatgtg
     3361 caagctcatg tgcaattaga ctcactaacg aacggtcgcc tgtaatgatg gtaggtgttg
     3421 caaatgttaa acatggattt tccattccac caaaaggaaa agaaggaggc agcaccaaaa
     3481 catcatattg tagccatggg tatttaccat acaattgttc tgccgaagaa atcatagcag
     3541 gcaaatctgt aaactcatga aaagctttgg ccaacataac aggttctgcg taaatgccgg
     3601 aacggttgtc gtaggctttg taagcaaggt cgccaatgct taaagccata agataggaag
     3661 gtacagcaaa aggcatttta aatgaatagg tgccattgcc aatttgttta ggattttgtg
     3721 gacaactcat caatgccata agttctttag gacaatgaat ggttgctgtc catgtaaatt
     3781 ttattccagg tgtatcctgc aaaggtatcc agctccgagc cagaatagct tccgattgtg
     3841 tatataaaaa aggatgtttt ttgcctgcag tttgttccgg gtttagccac tgtaatgctt
     3901 gtgcatcagg agaagtttca taataaatgg taacctcttt tgtatttgct gttataggaa
     3961 tagcaagttc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnngagcat cattgatttt tacactttca attttcaggt
     4081 tgtaggtgtc taaacgcaaa gtatcggcat tggtaagatt ttttacttgc aaagtcacaa
     4141 agccaactaa ctttttagtt gagaaatcag ctttaaggct taagtcaaga tgctgtacaa
     4201 caatttctgc tggattggaa tatgaatgtt gatctttaac gtatatccct ctttcatctt
     4261 ccggtttttt agcaaacatg gtaaatggta ttagaattaa aaataaaata tttttcattg
     4321 ttcaattatt ttgttttaat catataaata tgaggaatgt tatcttccag aaattcttcg
     4381 ctgatgacaa caaacccaaa tgactcataa aatttacgaa gataactttg tgccataatt
     4441 tttattgcac tattagggaa taagctttta cattcatcta aagatttttc gaccactaat
     4501 tttcccaatc ctgttcgtct gagttgttga tgtgtaacga tgcgcccaac agaaacttca
     4561 tcatacaaaa caccgggagg aataatacgt gcataaccaa caacttgttg atttgccata
     4621 gccatcagat gaaaagaaag tttatcggtt ttgtcaatgt caccataagg acaattttgc
     4681 tccacaataa aaacctgctg cctgagttgt aaaaaatcat gcagttcatg tgcagtgagg
     4741 tcattaaacg atttaaactt ccaaaaaata ttcatgctgt gatgggctga tatggcagcg
     4801 aatataaaac acattttatg aatagtaaaa gaagtaatac gattgaataa attgttagca
     4861 gcattttgat gattaacact atttacatca aactaaataa ataactttgg cgccatgtca
     4921 ttgccacaac tcattgttac tgacagcagc cgaaacggaa aacttccatt tgctcctttt
     4981 aaaaggacac aggaagaatc tgcacaacag ccaaagacag agaaggtaag caacaaagtt
     5041 gcgaagcaga aactttcttt aaaaacagac agcttaaaag gtaataccac tgtagttgat
     5101 acaataaaac caatagcaaa cgatagtatt caacttgcaa ttgattcttc agttgcagat
     5161 atgtctagat tgtttcgttt tacagatgag gaaaatgatg aactgaacaa ttcagatcaa
     5221 caatttattt catcatcggc atatttcaaa cagcagcagt tggaagtaaa atcgtcatct
     5281 ccacaattgt ttaataagat agttccggat tggtttacca tagtcctttt ttttctgatt
     5341 gtgggcatta caacagtaaa agttttttat cagaatattt tcagacaatt ggtcgaagct
     5401 gtttatagtt ttgcagtaac caatcaggtg gtgcgcgatg aaaatttgtt agtgcaacgt
     5461 gcttcagttt tgctaaatgt aattttttat tgtagctcag gtttgttttt ttattggatc
     5521 agtgtgcgct acaactggaa tcatccgatc ttaaatgaag gattgtttcg ttttttcttt
     5581 tttgctttcg tgacagcagt tttttttaca gtaaaaatgt tgttgctgaa actgataggc
     5641 acaattttta atctcgataa accggtggca gtttttattt tttccatttt tttgaccaat
     5701 aatttactag gagttctgct ggtgttgatt acttcattta tggcatattt atccggtgtg
     5761 ggtctgataa attacatcta tctggtatta ggaattcttt ctctggcatt tattttttta
     5821 ttgtacaggg catttttgat ttcccgtact gtgcagggtt attctttgta ttatttaatt
     5881 ttatactttt gcacccttga aattgtaccg attgcattga tagtaaaatt tgcatcggag
     5941 taattaaaca aggtgattaa aaacgcatgg ccggaaattc gaaagtaaag acaatactcg
     6001 taacacaacc caagccggaa acggaaaaga atccatactt tgaccttgca aaaaagttca
     6061 atgtaaaaat tgattttcgt ccttttattc agattgaagg aatacctgca aaagattttc
     6121 gcaaagacag agttaatgtt gcagaatttc aggcagtaat tttaaccagt cgccaggctg
     6181 ccgatcatta tttcagaatg gctgccgaaa tgcgcatgac aaattttgat gaagtaaaat
     6241 ttttctgcgt gtctgagtca actgcttttt atctccagaa atatatcaca tatcgtaagc
     6301 gcaaagtttt ttatggcaac aacagcatta acgatttgac agatgtgttg aagaaacata
     6361 agaaagaaaa gtttttgctt ccttgtagtg aagtaatgaa tcccgacatc attaacacat
     6421 taaaagacgg tgacctgaat tttataactg cagcaatgta cagaacggta agtagcgatt
     6481 tatctgatgt gaatattgaa acgtttgata tgattgtgtt cttcagccca tcgggagttc
     6541 agtcgctgtt gaagaatttt cctaaataca aacaaggcaa tacccgaatt gcaacattcg
     6601 gtgtcaccac atcacagact gcaaaagatg ctaagttaaa agtagaggtt gaagcacctg
     6661 ttccgcaggc accaagtatg acaatggcta ttgagcagta tctgaaaaaa gccaacaagt
     6721 aattttttac ttaatctcac ttagtttttg ctgtgcctgt gtagcgtaaa atcctttgct
     6781 ggaaattatt ttttgtaata actcttttgc tttatcctgc tcaccttttt gcaaaagcag
     6841 caatgctctg tgccacgctg attcctgatt aaatgtattg tttgactgtg catctaatcg
     6901 gtcgaggaag tgaagtgctt tatcatactg ctgactttcg ctgtatgaca ttgccgtata
     6961 aaacaaggca tttacatcgc tatgattgtc ggatagcaaa agttgcaact tattaattgc
     7021 agaggagtat ttcttattgt tgtaatcaac taatgcttca gctaaaaaat cttgatatcc
     7081 tattgtacga gactgcatgt tgttaagcgt tttggatttt tccatatcct ttctcgactc
     7141 ataacgggca ggaatgccgt tgtcttcttt tgctacgttc ttttcacgtt tgtactcacg
     7201 ggaatagtca tacactttta gtccttcaat attttttgtt accggcacta cagcatcaac
     7261 tacagcagca cctgccgatg cagactcagt aactattggt gcagcaacat ctgctgactg
     7321 tggctcagca attgcttcat ttttctgaac aggttctgtt gcagcaaagg ctttatcttc
     7381 tttacttgct tttgctacca ttgcattatg cgatgcaatt atgttttttg atgcttgttg
     7441 aattctttct tctgcaggag gaggtggtgg ttgaatggta gattcattca acttagtatc
     7501 actgctggtt tgaggtgttt ctgtatttat tttttgttgt acagcaggtg cgttaatttg
     7561 ttctgctgtg ttttcatgtt tgaaggaata gttagaaata atccatcctg tagcaattaa
     7621 aattgcaaca gatgctgcgg cataatacca tctgtttata gatggggatt gttttgcaga
     7681 agaagaatat ttatccgata cctgcttgat ggtttcatca agcggtttca tagaggtcaa
     7741 caaggcaaga ccttctgctg catcggaaca aagctcgcag tctgtcaggt gtttttcaac
     7801 ggcatacttt tcggcatccg tcatttttcc ctgcacatat tttgtcagac gttctttgtc
     7861 aacgcagtca gaggttttga atatttcaat tttacgactt aac
//
LOCUS       DAORQT010000059         7878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1355_length_7878_cov_7.921897, whole genome shotgun sequence.
ACCESSION   DAORQT010000059 DAORQT010000000
VERSION     DAORQT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1355_length_7878_cov_7.921897"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1247
                     /locus_tag="PK134_03455"
     CDS             237..1247
                     /locus_tag="PK134_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37935.1"
                     /translation="MLKKILIGFVIIIGLLCVGSLFISNNYKIERDIYIKAPASVVFN
                     QINNLKNWSKWNTWLKMDTTMKVTYNNIEAGVGAQYQWTSANKNVGNGSMQIKKSVQD
                     QSVESELLFEGEGNAVNGFNIQPTGDSVKLTTYFESEVGWNPLHKYMMLMFKGMMIDM
                     TDNGLKAIKELAENMPAQPGFEFNIQQVEMPSSIPYLATRCTSGLDSISYFIGSSYGL
                     IDTEMKKQKLEMSKTVHPFAIYYKWENNVFEYDVCMPLAAAGKSAGNIVAGEVKTGKY
                     LMTQYFGNYDNSNKAHEALVNYAKDNSIKISDAPLEFYITDPSVEKDTAKWQTDIYYQ
                     IN"
     gene            1484..3292
                     /gene="rpsA"
                     /locus_tag="PK134_03460"
     CDS             1484..3292
                     /gene="rpsA"
                     /locus_tag="PK134_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRB37936.1"
                     /translation="MSEVKHNVPPTDFDWDNVGKTADIYKSDDRQRMEAMYDQTLNTL
                     TEHEVKMGSIISISDKEVVVNIGFKSDGMVPLSEFRHMPDLKVGDEIEVYVETREDKN
                     GQLILSHKKARALKSWERVNEALEKDEIIQGYVKCRTKGGLIVDVFGIEAFLPGSQID
                     VKPIRDYDVFVGKTMEFKVVKINHEFKNVVVSHKVLIEEELESQKSEIMSKLEKGQVL
                     EGTVKNITSYGVFMDLGGVDGLLHITDISWGRINHPDEVLKLDDKINVVILDFDEDKK
                     RIALGLKQLTPQPWEALDQNIQVGDKIKGKVVVLTDYGAFIEVIPGVEGLIHVSEMSW
                     SQHLRSPQDFLKVGDEIETVILTLDREERKMSLGLKQLMPDPWVNIMEKYPVGSKHTA
                     KVRSLTNFGVFVELEEGIDGLIHISDLSWAKKIKHPAEFTKVGENIEVVVLEVDSEHR
                     RLSLGHKQLEENPWDTYETIFRVDTVHSGVVGKVTDKGATIIFPSFGVEGFAPYRHLM
                     KENGTPAKAEETLDFKVIEFSKENKKIVVSHSRLFEEAKASEKMARDNEKAKDESDAF
                     KSVKKIKDSQEKTTLGDLSVLSALKDEMKKNEAENN"
     gene            3455..4708
                     /locus_tag="PK134_03465"
     CDS             3455..4708
                     /locus_tag="PK134_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRB37937.1"
                     /translation="MPSVCFYFQVHQPFRIKHYSVFDIGKDHFYEDGEKNKWIMDKVS
                     EKCYLPANKKMLELIKRHKGKFRISYSLSGTVIEQMELFRPDVLQSFVDLSKTGCVEF
                     LSETYFHSLSFLYNKEEFERQIKEHDQKIEHYFKQKPTVFRNTELIYNNELAAFIEKM
                     GFKGILCEGVDRLLKDRHPNHLLKPTGTKSIKALLKNYRLSDDIAFRFSDKNWSEWPL
                     HADTFASWVHKVAGSGDVINLFMDYETFGEHQWESTGIFDFMDHMPREILKHPDFGFM
                     TPSQVVDAYTPRGVYDAHEFISWADTERDLSAWLGNNMQQEALNKIYSISDRVLGSGN
                     KDLIDVWGKLQTSDHFYYMSTKFWSDGDVHKYFSPYNSPYDAYIFYMNAFSDFESTLT
                     EEIKPNRKSAAKKPAAPRKKKPAAE"
     gene            4730..5617
                     /locus_tag="PK134_03470"
     CDS             4730..5617
                     /locus_tag="PK134_03470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRB37938.1"
                     /translation="MEHFRESLLILISTPIYIIVIGAEIFFSAFHHLQLYSKKGTLVN
                     LYLMLLNMGLDIIMRTASLLILNYFFDFRIINSYPTAWIYWLALFLAEDFMYYWLHRI
                     DHFCRLFWAVHVTHHSSEEFNLTVGFRSSVLQPLYRFIYFIPLSIVGFKAIDIMFIYS
                     ATQIFGILVHTQTVGKLGWLEYFLVTPSHHRVHHASNVCYLDRNMGMVLIIWDKLFGT
                     FQRELENEPIKYGLTNNLHSNNPGKIVFHEWQAIAHDLKKKTSVKNKIMYLLGPPGWS
                     HDGDCKTSAQLRKALLKKK"
     gene            complement(5624..6256)
                     /gene="msrA"
                     /locus_tag="PK134_03475"
     CDS             complement(5624..6256)
                     /gene="msrA"
                     /locus_tag="PK134_03475"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRB37939.1"
                     /translation="MTSLIAIILLTGCQNQAQSLREISNSTNMQSNIATDTATFGTGC
                     FWCTEAVFESLEGVISATSGYSGGNVADPTYEQVCSGNTGHAECIQVVYDSKKISYDD
                     LLQVFWESHDPTTLNKQGADVGTQYRSVIFYHNPQQKQLAESYKQKLNNENTFGKPVV
                     TEITAATTFYPAENYHQEYFKLNGHAPYCQFVIQPKVEKIRKIFSSKIKK"
     gene            complement(6320..6883)
                     /locus_tag="PK134_03480"
     CDS             complement(6320..6883)
                     /locus_tag="PK134_03480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRB37940.1"
                     /translation="MKLTIISASVREGRKSHRAALFFKNFLIKKYQADVSIADLNELN
                     FPIFEERLRFQKNKSPEVVQFAENIKQSDGVLIITPEYNGGYPASLKNVIDLMYDEWR
                     RKPIAIATVSDGMFGGMQVITSLQFILWKIRAWTVPAMFPMPQIDKNFDVDGTPVDVA
                     GTEKRATVFCDEWMWCIEANRRMQLNH"
     gene            6943..7518
                     /locus_tag="PK134_03485"
     CDS             6943..7518
                     /locus_tag="PK134_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37941.1"
                     /translation="MFSSEEIQHAISADFFNIKRAITDKVYSNFAELGTMAKEKLESD
                     FKIESTIINRSKIYRGENLEGLPWINCDAPGWFNKEETFAIRFLFWWANHYSVTLHLS
                     GKFLNMLDYDKMQSSKSENVLLCINEKPWRYDLHKDNYIPLAEIKDVKPRCRKMNFIK
                     LSIAYPIATLHDFNSQNKKALMCLIGLLRQN"
     gene            complement(7515..>7878)
                     /gene="prfC"
                     /locus_tag="PK134_03490"
     CDS             complement(7515..>7878)
                     /gene="prfC"
                     /locus_tag="PK134_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814563.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRB37942.1"
                     /translation="IEQLTDEGVAQLFIQNGGNRKIIGTVGELQFEVIQYRLQNEYGA
                     SCKFITQNISKACWLTSKDKNKLDEFMRFKMNNIAFDKDQNPVYLADSQWMLDQMIKN
                     YPEVQFHFTSEFKMEVAV"
BASE COUNT         2554 a         1345 c         1474 g         2505 t
ORIGIN      
        1 aatcattact ttaaataaga taattaacaa ttgctgattt ccgtaaagac catgctttct
       61 tcacagaggt taaaaattta catattattt atttaacata atatattgca tttgtggaat
      121 attatggtaa cccctattat ttatagggtt tttcgttttt tttagtttgt atatgaatat
      181 tttgtaagat aatttacact acttttaccc tcgtaaaacc actaaatcaa attgttatgc
      241 taaagaagat actcattgga tttgtcatca tcatcggatt gttatgtgtt ggctccctat
      301 tcatctccaa caactataag attgaacgcg atatttatat aaaagctcct gcttctgttg
      361 tgtttaatca gattaacaat ctcaaaaact ggagcaaatg gaatacctgg cttaaaatgg
      421 atacgacaat gaaagtcacg tataacaata tcgaagccgg tgttggggct caatatcagt
      481 ggacatcagc taataaaaat gttggaaacg ggtccatgca gattaaaaaa agtgtgcaag
      541 atcagtctgt agaatcagag ctccttttcg aaggtgaagg aaatgctgta aatggtttta
      601 atattcaacc taccggtgac agcgttaaac ttactactta ttttgaaagt gaagttggct
      661 ggaatcctct acataagtat atgatgctta tgtttaaagg tatgatgatt gacatgacag
      721 acaatggctt gaaagcaatt aaagagttag cagaaaacat gcctgcacaa cccggatttg
      781 agtttaatat tcaacaagtt gaaatgcctt cgtctattcc atatttagct accagatgta
      841 cttcaggctt agattcaatc agctatttta ttggctcaag ttatggtttg atagatacag
      901 aaatgaaaaa gcagaaatta gaaatgagta agactgttca tccttttgca atttattaca
      961 agtgggaaaa taatgttttt gaatatgatg tttgtatgcc attggctgca gcaggaaaat
     1021 cagccggaaa tattgttgct ggcgaagtta aaacaggaaa ataccttatg actcagtatt
     1081 ttggcaatta tgataattcc aacaaagctc atgaagcact cgtaaactac gctaaggata
     1141 acagcataaa aatctctgat gcacctcttg aattttacat tacagaccct tctgttgaga
     1201 aagataccgc caagtggcag acagatattt actatcagat taattgagcc tgctctaaaa
     1261 gcataaagat taagggttaa accgattggt ttagcccttt tttattggtt ttttgggaat
     1321 ttcaggctgc agaaccttca acaaccggaa ctgaataatt atcaggcata ttagttttat
     1381 ttatcaaagc aaatacctat ctttgcgcgc ccgttttaaa aggtatattc ataaacaata
     1441 aataaagtct aacaatttca attattaaaa aacaaaacaa ttaatgagtg aagtaaaaca
     1501 caacgttcca ccgacagatt tcgattggga caacgttggc aaaacagccg acatttacaa
     1561 atcagatgac cgccaacgta tggaggccat gtacgatcaa acccttaaca cacttacaga
     1621 acatgaggta aaaatgggtt ctatcatcag catttctgat aaagaagtgg tggtaaacat
     1681 cggattcaaa tcagatggaa tggttccact gagcgaattc cgtcacatgc ctgacttaaa
     1741 agtgggcgat gaaattgaag tgtatgtaga aacccgtgaa gataaaaacg ggcaactgat
     1801 attatcacat aaaaaagcac gtgctcttaa atcatgggag cgtgttaatg aagcccttga
     1861 aaaagatgaa attattcagg gttatgttaa atgccgtact aaaggcggac ttatcgttga
     1921 cgtatttggt attgaagcat ttttaccggg ttcgcaaatt gatgtgaaac caatccgcga
     1981 ctatgatgtg tttgttggaa aaactatgga atttaaagtg gttaaaatca accatgagtt
     2041 taagaacgta gttgtttctc acaaagtact tatcgaagag gaacttgaaa gccaaaagtc
     2101 agaaatcatg tctaaacttg aaaaaggaca ggttcttgaa ggtacagtta aaaacatcac
     2161 gtcttatggt gtatttatgg accttggtgg cgttgacggt ttattgcaca ttaccgatat
     2221 ttcatgggga cgaatcaacc atcctgatga agtgttgaag ttagatgata aaattaatgt
     2281 tgtaatcctt gattttgatg aggataaaaa acgtattgct cttggcttga aacagcttac
     2341 acctcagcca tgggaagcac ttgaccaaaa cattcaggtt ggcgacaaaa tcaaaggaaa
     2401 agttgttgtg ttgactgact atggtgcttt cattgaagta attccaggtg ttgaaggatt
     2461 aattcacgtt tcggaaatgt catggtcaca gcatttacgc agcccgcaag actttcttaa
     2521 agttggtgat gaaatagaaa ctgtaatcct tactcttgac cgcgaagagc gtaaaatgtc
     2581 tcttggctta aagcaactta tgcctgatcc atgggttaac attatggaga aatatcctgt
     2641 tggctcaaag catactgcaa aagttcgcag cctgacaaac tttggtgttt ttgttgagtt
     2701 agaagaaggt attgatggtt taattcatat ctctgacctt tcatgggcta agaaaattaa
     2761 gcatcctgct gagtttacca aggttggcga aaacattgaa gttgtagttc ttgaagttga
     2821 ttcagagcac cgcagattaa gtcttggaca taagcagtta gaagaaaatc cttgggatac
     2881 ttacgaaaca attttccgtg ttgacactgt tcattcaggt gttgttggaa aagtaaccga
     2941 taaaggtgct actatcatat tcccatcatt tggcgttgaa ggttttgctc catatcgtca
     3001 tctgatgaaa gaaaacggta ctcctgctaa agctgaagaa acacttgatt ttaaagtaat
     3061 cgaattcagc aaagagaaca agaagattgt tgtttctcac agccgcttgt tcgaagaagc
     3121 aaaagcatct gaaaagatgg cgcgcgacaa tgagaaagct aaagatgaaa gtgatgcatt
     3181 taagtcagta aagaaaatca aagattctca ggaaaaaaca accttgggtg atttgagcgt
     3241 tctttctgca cttaaagacg aaatgaagaa gaacgaagca gaaaacaatt aatctgctta
     3301 ttcatcaata aaatcaaaaa gccgctttta gcggcttttt gatttatgta ccatcccttt
     3361 aatcataaat ttatgatgac agtttcagga tattttcttt tgtttcaggc aaaagtttaa
     3421 ttttggcaca acgaccattt ttaaaaacaa atttatgcca tcggtttgct tttactttca
     3481 ggtgcatcag cccttcagaa taaaacatta cagtgttttc gacattggta aagaccattt
     3541 ttatgaagat ggcgaaaaaa ataaatggat tatggacaag gtatctgaga aatgttacct
     3601 tcctgccaat aaaaaaatgc ttgagctgat taagcgtcat aaaggtaaat tcagaataag
     3661 ctattcacta agtggaacag tgatagaaca gatggaattg ttcagaccgg atgttcttca
     3721 gtcatttgtt gatttgtcta aaacaggttg tgtagaattc ctctctgaaa catattttca
     3781 ttcactcagt tttttatata acaaagaaga gtttgagaga caaataaagg agcatgatca
     3841 gaaaattgaa cattacttta aacagaagcc aactgttttc agaaataccg aattaattta
     3901 caacaatgag cttgctgcct ttattgaaaa aatgggcttt aaagggattt tgtgcgaagg
     3961 tgttgatcgt ttattgaaag acagacaccc taatcattta ttgaaaccaa caggaacaaa
     4021 atcaataaaa gcattactta aaaattatcg tttgagcgat gacattgctt ttagattctc
     4081 tgacaaaaac tggagcgaat ggccactaca tgccgataca tttgcaagtt gggtacacaa
     4141 agttgcaggt agtggtgatg tgataaacct gtttatggat tatgaaactt ttggcgagca
     4201 tcagtgggaa agcacaggca tttttgattt tatggatcac atgcctcgtg aaatattaaa
     4261 gcatcctgac tttggcttta tgacaccatc acaggttgta gatgcttata cgcctcgcgg
     4321 tgtatatgat gcacatgagt ttatcagttg ggcagatact gaacgcgatc tttcagcatg
     4381 gctgggaaat aatatgcaac aggaagcgct caataaaatt tattcaataa gtgacagggt
     4441 gcttgggtca ggaaacaaag atttaattga cgtttggggt aagctacaaa ccagcgatca
     4501 tttttattat atgagtacta agttctggag tgatggcgat gtacataaat atttcagccc
     4561 ttataactca ccttacgatg cttatatttt ttatatgaat gcattttctg actttgaatc
     4621 tactttaaca gaagaaatta agcccaatag gaaatctgct gcgaaaaagc cggcagcacc
     4681 gcgtaaaaag aagcctgctg ctgaataaaa ttttacttca gtattttata tggaacattt
     4741 cagagagtcg ttgctgattc tgatttcaac acctatttac attatagtta ttggtgctga
     4801 aatatttttt tctgcatttc atcatttaca actctattca aaaaaaggaa ctcttgtcaa
     4861 tctctatctc atgttgctga atatgggact cgatattata atgcgtactg caagtctgtt
     4921 aattctgaat tattttttcg atttcagaat tattaactcc taccctactg catggattta
     4981 ctggttagct ttatttcttg cagaagattt tatgtattat tggctgcatc ggatagacca
     5041 tttttgtcgc ctattctggg ctgtacatgt cactcatcac agcagcgaag agttcaacct
     5101 tactgttgga ttccgttctt ctgttttgca accactttat cggttcattt attttattcc
     5161 acttagcatc gttggcttta aggctattga cattatgttt atttatagcg cgacacagat
     5221 ttttggaata ttggttcaca cgcaaaccgt tggcaaatta ggatggcttg aatattttct
     5281 tgtaactcca tcacatcaca gagttcacca tgcaagcaat gtttgttatc ttgacagaaa
     5341 catgggaatg gttttgataa tctgggataa attgtttggt acttttcaga gggaattgga
     5401 aaatgaaccc attaagtatg gtctgacaaa taatttacat tctaacaatc cgggaaaaat
     5461 tgtatttcac gaatggcagg ctattgcaca tgatttgaaa aagaaaacat ctgttaaaaa
     5521 taaaattatg tacttactcg gtcctccggg ttggagtcat gatggcgact gtaaaacaag
     5581 cgcacaacta cgcaaagcat tattgaaaaa gaaatagctt cagttatttt ttaatttttg
     5641 aactaaaaat ctttctgatt ttttcaacct taggttgaat aacaaactga cagtatggtg
     5701 cgtgaccatt cagtttaaaa tattcctgat gataattttc agcaggataa aatgtagttg
     5761 ctgctgttat ctcagtcaca acaggcttgc caaaagtatt ttcgttgttc agtttttgct
     5821 tgtacgattc tgctaactgc ttttgctgtg ggttatgata aaatattaca gaacggtatt
     5881 gtgtacccac atcagcgcct tgtttgttta atgttgtagg gtcatggctt tcccaaaata
     5941 cttgcaaaag gtcatcataa gaaatttttt tagagtcata aactacctga atacattctg
     6001 catgacctgt gttgccactg cacacctgct cataggttgg gtctgcaaca ttgccaccgg
     6061 aataacctga agttgccgag attactcctt caagagattc aaaaacggct tctgtacacc
     6121 aaaagcatcc tgttccgaaa gttgctgtgt ctgtggctat gttgctttgc atatttgtag
     6181 aattagaaat ttcccgtagt gactgtgcct gattctgaca acctgtaagc aaaattattg
     6241 cgattaaact tgtcatgtaa tacatttttt gaatattatc aggaataaac aaattaaaat
     6301 tactttggtt taaattcatt taatgattca actgcattct tctgtttgct tcaatacacc
     6361 acatccattc atcacaaaat actgtagctc ttttttctgt gcctgcaaca tcaaccggtg
     6421 ttccgtcaac atcaaaattt ttatctatct gcggcattgg aaacatagca ggtacagtcc
     6481 atgctcttat tttccataaa ataaactgca aggaagtaat tacttgcatt cctccaaaca
     6541 ttccatcaga aacagttgca atggctatag gctttctgcg ccattcatca tacatcaggt
     6601 ctattacatt ttttaatgat gccggatagc ctccattata ctcaggcgta attattaaaa
     6661 caccatcaga ctgtttaatg ttttctgcaa attgaacaac ttcaggagac ttgtttttct
     6721 gaaatctaag tcgctcttca aatataggga agttaagttc attcagatcg gcaatgctaa
     6781 catctgcctg atattttttg atcagaaaat ttttaaaaaa taatgctgca cgatggcttt
     6841 tgcgcccttc tcgtacactg gcactgatta tagtaagttt cattcaacaa ttaatttaca
     6901 acacaaataa aacgaaaaag taattgctat cttcacggac ttatgttttc atcagaagaa
     6961 atacagcatg ccatctctgc tgactttttt aatattaaaa gagccatcac agataaggta
     7021 tacagtaact ttgcagagtt aggcactatg gctaaagaaa aactggaaag tgattttaaa
     7081 atagaatcta caattataaa tcgcagcaaa atctacaggg gagaaaatct tgaagggtta
     7141 ccttggataa attgtgatgc tccgggatgg tttaataagg aggagacttt cgcaattcgc
     7201 tttttgttct ggtgggctaa tcattacagt gtgacacttc atttaagtgg caaatttttg
     7261 aatatgcttg actatgataa aatgcaatcc tctaaatcag aaaatgtatt gttgtgtatc
     7321 aatgaaaaac cttggcgcta tgatttacat aaagacaact acattccatt ggctgaaatc
     7381 aaggatgtaa aaccgagatg ccggaaaatg aattttataa aactctcaat tgcatatcca
     7441 attgcaactt tacatgattt taattctcag aataaaaaag cacttatgtg tctgatagga
     7501 ctacttaggc agaattaaac agcaacttcc attttaaatt cgctggtaaa atgaaattgt
     7561 acttcaggat aattctttat catctggtct aacatccatt gtgagtcggc aagataaaca
     7621 ggattttgat ctttatcgaa tgcaatgttg ttcattttaa agcgcatgaa ttcatctagc
     7681 ttattcttgt cttttgaagt gagccaacag gcctttgata tattctgtgt tataaattta
     7741 caagatgctc catattcgtt ctgaagtctg tattgaataa cttcaaactg aagttcgcca
     7801 actgtaccaa taatttttct gttaccacca ttctgaataa acaactgcgc cactccctca
     7861 tccgttagct gttcaatt
//
LOCUS       DAORQT010000060         7728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1401_length_7728_cov_5.232504, whole genome shotgun sequence.
ACCESSION   DAORQT010000060 DAORQT010000000
VERSION     DAORQT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_7728_cov_5.232504"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1622
                     /locus_tag="PK134_03495"
     CDS             3..1622
                     /locus_tag="PK134_03495"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRB37943.1"
                     /translation="MALAQLNFHVGNFENNTSKIIEAIKQAEKSGAELIVFPELAITA
                     YPPLDFLEFDDFINRCYNSIEKIAAHSQNIAVIVGCPSRNAVPEGKNLFNTAWFLHKG
                     KVQALVNKALLPTYDIFDEYRYFEPGRKFECIEFNGEKIALTICEDLWNVEDDPLYIH
                     SPMEELIKEKPSLIINIAASPFDYKHAHQRKAILQRNVNQYKIPLLYVNHVGAQTEII
                     FDGGSLAYDASGKLVHEGPYFKEEIIYLNTKNTTLVVEKQTEKYELIYQALLMGIRDY
                     FSKMGFKKAILGLSGGVDSAIVTVLAADALGAENILPVMLPSAFSSGHSISDSEQLCQ
                     NLNIKVDKMEINPLYETFLKTLKPLFGEKPFDVTEENIQARIRGTLLMALSNKFGPIL
                     LNTSNKSELAVGYGTLYGDMCGGLSVIGDLYKTEVYELCRYINRKKEIIPENILSKAP
                     SAELRPGQKDSDSLPPYDILDKILFEYIELRKGPKEIIKGGYDETLVNRILSMVNRNE
                     YKRKQLSPVLRISSKAFGLGRRMPIVGKYLS"
     gene            complement(1625..2038)
                     /locus_tag="PK134_03500"
     CDS             complement(1625..2038)
                     /locus_tag="PK134_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37944.1"
                     /translation="MKNIALYILTFGMTVFLASCHKDDSCNAGTGGNNSIKATLLHHT
                     KVISPKVGYPDTLFIKYDTKDFPGENASDFDAYFVNSDTAISTIEITGLKCGDYYLFG
                     TGWDADISQRVKGGIPVTIAEDAGVLTKNVPVTED"
     gene            complement(2061..3071)
                     /locus_tag="PK134_03505"
     CDS             complement(2061..3071)
                     /locus_tag="PK134_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB37945.1"
                     /translation="MIKIQVSKIFILSVIVFWIVSIVSCRKDDAIGTGNEVLELEVPL
                     GFPYPSIPEDNQPTKNRIALGKILFFDPILSRDSTISCSSCHHADKKFTDGLKVAEGI
                     EGRKTLRNSMTILNTAYQPNMFWDGGSPTLETQVSAPIEAHEEMDFNVMKVVERLNNH
                     PLYPSLFRKAYNSGPSAFTLTRAIANFERTLFTGKSKYDAFQYEKKSDALSASEKRGM
                     DIFFGERAECFHCHGGFNFTDFSFKNNGLYEVYADSGRARITTLHDDVGEFKVPSLRN
                     VALTAPYMHDGSVATLEDVVEHYNSGGKPNFNKSFLIRPLNLTQQEKEDLVNFMKALS
                     DQ"
     gene            complement(3068..3832)
                     /locus_tag="PK134_03510"
     CDS             complement(3068..3832)
                     /locus_tag="PK134_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37946.1"
                     /translation="MKSIKSVLCLAVVGFIFSFSSCKKDDSDANNIPNTDTIGELSFN
                     FRQFVGNDRIVYSQVYNDTSGRPFKITNLRYYISNIVLIKANGLELPLSGKVLLVDNE
                     KEQYTLGEIPVGDYSGLRFMVGIDSATNHLDPALYDASNPLSYQPVSMHWGWNFGYIF
                     MKVEGNVDTDSVPTGNLNGIISYDIGSDPLVRVINFNHIFSVHGNINTVNFKFNILKM
                     FSGIDLATESITHTNPGRDLADRIASNWLLAFTIEP"
     gene            complement(3866..4606)
                     /locus_tag="PK134_03515"
     CDS             complement(3866..4606)
                     /locus_tag="PK134_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37947.1"
                     /translation="MKKRILITLAVSTLFFTACKKDKDSKPAESELSFHLHTLVGNTA
                     ANYTDIFTDNTGRKFNIADCRYYISNIVLIKDDGTEFPLTDKALLASPSQKEYSLGNV
                     PVGKYKGFRFLMGLDSVTNHDDPAGYAASNPLSYQNPTIHWGWNNGYIFMEFVGKFDS
                     TVAATGNPVADYFYHIGFDDLKRTIDFSNSPFEITTDAGLEIGILFDLKKVLNNVDLR
                     TEFGTHTMDNMPLAVKIANNWQGAYSIE"
     gene            complement(4655..6217)
                     /locus_tag="PK134_03520"
     CDS             complement(4655..6217)
                     /locus_tag="PK134_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRB37948.1"
                     /translation="MADKEITQPIQTDSGIEVKQVYTSCKPMDELPAKFPFTRGVQAD
                     MYRSKLWTMRQYAGFSTAEESNKRYHYLLKQGTTGLSVAFDLPTQIGYDSDHELSEGE
                     VGKSGVAIDSLRDMEVLFDGIQLQNITTSMTINSTAAVLLSMYIALAKKQGADLSKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFDFCSREVPKWNTISVSGYHIREAGST
                     AVQELAFTLADGKAYLKAAIDAGLDINVFAKRISFFFNAHNNFFEEIAKFRAARRMWA
                     SITKELGATDPKAMMLRFHTQTGGSTLTAQQPQNNIMRVTMQALSAVLGGTQSLHTNG
                     FDEALSLPTENAARIALRTQQVIAYESGVTQTVDPLGGSFYIESLTDEVEAKAWDYIR
                     KIDDMGGAVAAIEAGYIQNEIAESSYNYQNAIQTGEKVIVGVNKFQVEEPVFDDIFSV
                     DDTIRKVQVQKLAQLRTERNNEKVNQALSNLESAAKDNANLMPYIIQCVEHYTTLGEI
                     SDKLRKVFGEYR"
     gene            6368..7099
                     /locus_tag="PK134_03525"
     CDS             6368..7099
                     /locus_tag="PK134_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB37949.1"
                     /translation="MKLPKGISDLLHETGQLSSFVVRFFKEGFKPRYEWEELFKQAFI
                     NGYKSLPLVGITAFIMGLVLTVQSRPTLAEFGATSWLPAMIAISIVREIGPVITALIC
                     AGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKFLVVTRVLSIAFMLPILVILADLIA
                     IYACYIGVNFKDVVSWKLFYNKVFNSLEYSDFIPALVKSFFFGLTIGLVGCFKGYNSQ
                     KGTEGVGHAANSAVVVASFIIFILD"
     gene            7118..>7728
                     /locus_tag="PK134_03530"
     CDS             7118..>7728
                     /locus_tag="PK134_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB37950.1"
                     /translation="MMDNTNIILKIEGLCKAFGSNVVLNNFNLELIKGENIVVLGKSG
                     SGKSVLIKCIVGLLKPDKGNIHVFDTNMLELDQESLDKIRIKIGFLFQNSALYDSMTV
                     RENLEFPMRRHWISLTSSEVKNLVEESLDNVGLLHAINMMPSELSGGMRKRIGIARTL
                     IMKPKLILYDEPTTGLDPITSREIVQLMLDLQKKYHTSSVIIS"
BASE COUNT         2391 a         1429 c         1416 g         2492 t
ORIGIN      
        1 acattgcact tgcccaactc aactttcatg tgggcaactt tgagaacaac acttcaaaaa
       61 ttattgaggc aatcaaacaa gccgaaaaat ccggtgcaga actgattgtt tttcccgaac
      121 tggctataac agcatatcct ccactcgact ttctggagtt tgatgatttc atcaaccgtt
      181 gttataacag catagaaaaa attgcagcac acagtcagaa tattgcagtc attgttggtt
      241 gcccatcgcg caatgccgtt cccgaaggga aaaatctctt taacactgca tggtttttgc
      301 ataaaggcaa agttcaggct ttagttaaca aagcactatt gcctacatat gatatttttg
      361 atgagtatcg ctatttcgag ccgggcagaa aatttgaatg tattgagttt aacggagaaa
      421 aaattgcttt aactatttgc gaagatttat ggaatgtgga agacgatcca ctctacattc
      481 attctcctat ggaagagttg ataaaagaaa aaccttcttt aatcatcaat attgcagctt
      541 cgccatttga ttacaaacat gcacatcagc gaaaagcaat tttgcagcga aatgtaaatc
      601 agtataaaat tccattattg tatgtgaacc atgttggcgc acaaactgaa attatctttg
      661 atggtggctc attagcttat gatgcttcgg gtaaattggt gcatgagggg ccttatttta
      721 aagaagaaat aatttatctg aatacaaaaa acaccactct tgttgttgaa aagcaaacag
      781 aaaaatatga attgatttat caggcattac tgatggggat aagagattat ttttctaaga
      841 tgggttttaa gaaagcgata cttggcttgt caggtggtgt ggacagtgcc attgttacag
      901 tgttggcagc cgatgccttg ggtgcagaaa atattttgcc agttatgttg ccttcagctt
      961 tttcaagcgg acattcaata tcagactccg aacaactttg tcaaaacctg aacatcaagg
     1021 ttgataaaat ggaaataaat ccattgtatg aaacgttttt gaaaacactt aaaccattgt
     1081 ttggtgaaaa gccttttgat gttactgaag aaaacattca ggcacgcata cgcggaacat
     1141 tgctgatggc actgagtaat aaatttggcc ctatactttt aaacacaagc aataaaagtg
     1201 aacttgccgt tggctatgga actttatatg gcgacatgtg tggcgggctg agtgtgattg
     1261 gcgatttgta caaaacagaa gtttatgaat tgtgcagata catcaacaga aaaaaagaaa
     1321 tcattcctga aaatatttta agtaaagctc caagtgcaga attacgtccg gggcaaaagg
     1381 acagtgattc tttaccgcct tatgatattc tcgataaaat tttgtttgaa tatatagaac
     1441 tgcgtaaagg cccgaaagaa attataaaag gcggatatga tgaaacttta gtaaaccgta
     1501 ttttgagtat ggttaaccgc aatgagtata aacgcaaaca gttgtcgcct gtattgcgca
     1561 tttcatctaa agcgtttggc ttaggcagac gaatgcctat tgtgggaaaa tatctttctt
     1621 agtactaatc ttccgtaacc ggaacattct ttgtcagcac gcctgcatct tctgcaatag
     1681 ttaccggaat gccaccttta acacgttgag aaatatcagc atcccaaccg gttccaaaca
     1741 aataataatc accacatttt aaacctgtaa tttcaatagt gcttatagca gtatcgctgt
     1801 taacaaagta agcatcaaaa tcagaagcat tttcacccgg aaaatctttg gtgtcgtatt
     1861 ttataaaaag tgtatcagga tatcccactt ttgggcttat aaccttagtg tgatgcaaca
     1921 aagttgcctt tatagaatta tttccaccgg taccggcatt gcaactgtcg tctttatggc
     1981 atgatgcaag gaatactgtc attccaaaag ttaaaatgta taatgcaata tttttcatga
     2041 tgctaaatta aatgagattg ttattgatcg gataatgctt tcataaaatt caccaagtct
     2101 tctttttcct gttgtgtaag attcaatggc ctgatgagaa aacttttatt aaaatttggc
     2161 ttacctcctg agttataatg ttcaacaaca tcttcaagag tagccacact gccatcgtgc
     2221 atatagggtg ctgtgagtgc aacattgcga agtgatggca ctttaaactc tcccacatca
     2281 tcatgcaaag ttgttatacg tgctctgccg gaatctgcat aaacttcata aagtccgtta
     2341 ttcttaaagc taaaatccgt aaaattaaat ccaccatgac aatgaaaaca ttctgctctc
     2401 tcaccaaaaa atatatccat acctcttttt tctgaagcag acaatgcatc gctttttttc
     2461 tcatactgaa acgcatcata ctttgatttg cctgtaaaga gtgtacgttc aaaatttgca
     2521 atggctcttg taagtgtaaa tgctgatggt ccgctgttat atgctttcct gaacaatgat
     2581 ggatataaag gatggttatt taaacgttca acaactttca tgacattaaa atccatttcc
     2641 tcgtgcgctt caattggtgc agaaacctgt gtttccaatg tcggtgagcc accatcccaa
     2701 aacatatttg gctgataagc cgtgttgaga attgtcattg aattgcgtaa tgtctttcgt
     2761 ccttcaattc cttcagcgac tttaagtccg tcagtaaatt ttttatcagc atgatggcaa
     2821 ctggaacaag aaatggtact gtcgcgagag agtattgggt caaaaaacag aattttgcct
     2881 aaggcaattc tgttttttgt aggttgatta tcttccggta ttgaaggata gggaaatcct
     2941 aaaggtactt ccaattccag tacttcgttt cctgtgccaa tggcatcatc ttttcggcat
     3001 gatacaatgc ttactatcca aaaaacaata actgataaaa taaaaatttt tgatacttgt
     3061 attttaatca tggttcaatg gtgaaagcaa gaagccaatt gctggcaatt ctgtctgcta
     3121 aatctctacc cggatttgta tgtgtgatgc tttctgttgc taaatcaata ccactaaaca
     3181 ttttcaggat attaaattta aagttgactg tgttgatatt accatgcaca gaaaaaatat
     3241 ggttgaaatt tatcacacgc accaatggat cgctaccaat atcgtatgaa attattccgt
     3301 ttagatttcc tgtaggaaca ctatctgtat ctacattgcc ttctactttc ataaaaatat
     3361 atccaaagtt ccaaccccaa tgcatactca cgggttgata acttaacgga tttgatgcat
     3421 cgtacaatgc agggtcgagg tgattggttg cagaatcaat accaaccata aaccgcaaac
     3481 cggaataatc accaacagga atttcaccaa gagtatattg ctctttttca ttgtcaacca
     3541 acagcacttt tccactaagc ggaagttcca gaccatttgc ctttattaaa acaatatttg
     3601 aaatataata acgcaggttg gtaattttaa agggtcgccc tgatgtgtca ttataaacct
     3661 gactgtaaac tattctgtcg ttgccaacaa actgtctgaa gttaaaagac aactcaccaa
     3721 tggtgtcggt gttgggtata ttattagcat ctgaatcatc tttcttgcaa gagctaaaag
     3781 agaaaataaa accaacaaca gctaaacaca aaacagactt gatacttttc atgatagaaa
     3841 atattaatac atcgaagata gtaaattatt caatgctgta agcaccctgc cagttgttgg
     3901 caattttaac agcaaggggc atattatcca ttgtatgtgt accaaactct gtacgtaaat
     3961 ctacattgtt cagtactttt ttaaggtcga ataaaatacc aatctctaaa ccggcatcag
     4021 tagtaatttc aaaaggagaa ttgctaaaat caatagtacg ctttaaatca tcaaaaccaa
     4081 tgtgataaaa ataatctgcc accggatttc ctgttgcagc gactgttgaa tcgaacttgc
     4141 ctacaaactc cataaagatg tagccattgt tccagcccca atgtattgtt ggattctgat
     4201 aactcaaagg atttgatgca gcataacctg cagggtcgtc atgattagtc actgagtcta
     4261 accccattaa gaaccgaaaa cctttatatt ttccaaccgg aacatttccc aaactgtatt
     4321 ctttttgtga tggacttgcc aatagcgctt tatcggtcaa cggaaattcc gttccatcat
     4381 ctttaatcaa aacaatgttg ctgatgtaat atctgcaatc agcaatatta aattttcttc
     4441 cggtgttatc agtaaagata tcggtgtagt tggcagcagt gtttcctact aaggtatgca
     4501 gatgaaacga taactcgctt tcagcaggct tgctgtcttt atctttctta caagcagtga
     4561 aaaataaagt tgaaacagca agagtaatta aaattctttt tttcattttt aaaaattata
     4621 tatgatttga attttacaat gtcattaaat tttatcaacg atactcacca aaaacttttc
     4681 tgagtttatc ggatatttct ccaagtgttg tatagtgttc aacacattga ataatgtaag
     4741 gcataagatt ggcattatcc tttgcagcag attctaagtt gcttaatgcc tgattaactt
     4801 tttcattatt tctctctgtg cgcaactgtg caagtttttg tacttgtact tttctgatgg
     4861 tgtcatcaac agaaaagata tcatcaaaaa ccggttcttc aacctgaaat ttatttactc
     4921 ccacaattac tttttctccc gtttgaattg cattttgata attatatgac gactctgcaa
     4981 tttcattctg aatatagcct gcttcaattg ctgcaacagc gcctcccata tcatcaattt
     5041 ttctgatgta atcccaagcc ttagcttcta cttcatcagt taatgattct atataaaaag
     5101 aaccaccaag cggatctacc gtttgagtta caccactttc ataagcaata acctgctgtg
     5161 ttcgcaatgc aatacgtgca gcattttcag tcggtaatga taatgcctca tcaaaaccat
     5221 tggtgtgcaa cgattgtgtt ccaccaagta cagcactcaa tgcctgcatg gttacacgca
     5281 taatgttgtt ttgtggttgt tgtgctgtta atgtactgcc tccggtttgt gtatgaaaac
     5341 gcagcatcat tgctttaggg tctgtagcac caagctcttt tgtgatagat gcccacattc
     5401 tacgggctgc acgaaacttg gctatctctt caaaaaaatt gttgtgtgca ttgaagaaaa
     5461 aagaaatacg tttggcaaaa acatttatat ccaatcctgc atctatcgct gctttaagat
     5521 aagccttgcc atcagctaag gtaaatgcca actcttgcac agcagtgctt cctgcttcac
     5581 gaatatgata accggaaaca gatatggtat tccacttagg aacttcacga ctgcagaaat
     5641 caaaaatatc tgtaatgatg cgcatgcttg gtttaggcgg ataaatgtat gtgccgcgtg
     5701 ctgcatattc tttcagaata tcgttctgta ttgttcctga tattttagag aggtctgctc
     5761 cttgcttctt cgccaaagca atgtacatgc tcaacaaaac agcagccgtt gaattgatgg
     5821 tcatagatgt tgtgatgttc tgaagctgaa taccgtcaaa caatacttcc atatcgcgca
     5881 atgaatctat tgccacaccg gactttccta cttcaccttc tgaaagctcg tgatcggagt
     5941 cgtagccaat ttgtgtaggc aaatcaaaag caactgataa gcctgttgtg ccttgtttca
     6001 gcaaatagtg atagcgttta ttagattctt ctgcagtaga aaatcctgca tactgccgca
     6061 ttgtccacaa cttgcttcta tacatatcgg cttgcactcc acgtgtaaat ggaaactttg
     6121 caggtagttc gtccattggt ttgcatgatg tataaacctg ttttacttca atgccggaat
     6181 cggtctgtat gggttgggta atttctttat ctgccattgt gcaaagatgc taagaaaact
     6241 ctgtttttag aaatgtagta aattgtggtt aacctacagg tttattcact ttattaaact
     6301 attgctccga ttaatttttt atcttagctc ctttatgaaa gtgtttttct tacttggaat
     6361 ttgaattatg aagctgccta aaggtatatc tgatttatta catgaaacgg ggcaattgtc
     6421 gtcctttgtt gttcgttttt tcaaagaagg atttaaacca cgctatgaat gggaggaact
     6481 ttttaaacaa gcatttataa acggctataa gtcgttgcca ctagtaggta ttacagcatt
     6541 cattatgggt cttgtactta ccgtacaatc gcgacccaca ttagcagaat ttggtgcaac
     6601 atcatggttg ccggcaatga ttgctatttc aattgtacgg gaaatagggc cggtaataac
     6661 tgcattaatc tgcgcaggta aaattggctc aagcattgga gctgaattgg gttccatgaa
     6721 ggttactgaa cagattgacg ctatggaagt ttctggcaca aatcctttta aatttttagt
     6781 tgttacacgc gttttgagta ttgcatttat gttacccata cttgttatac tggctgattt
     6841 aattgctatt tacgcctgct atattggtgt taattttaaa gatgtagtaa gttggaaatt
     6901 attttataac aaggttttca attcacttga gtacagtgat tttattcctg cattagtcaa
     6961 atcattcttt tttggtttaa ccatcggact tgtaggttgt tttaaaggtt ataattctca
     7021 aaaaggaaca gaaggagttg gacatgctgc aaattctgct gtagtagtag cttcatttat
     7081 tatttttatt cttgactgat ttattaggct tgaactgatg atggacaata ctaatatcat
     7141 tctgaaaata gaaggacttt gtaaagcatt tggcagtaat gtagttctga ataattttaa
     7201 tctggaatta attaagggag aaaatattgt tgtattagga aaatcaggct caggaaaatc
     7261 tgtactgata aaatgtattg ttggattact gaaacctgac aaaggtaata ttcatgtttt
     7321 tgacacaaac atgcttgaat tagatcagga gtcgctagat aaaattcgta ttaaaattgg
     7381 atttcttttt cagaatagtg cattgtacga ctcaatgaca gtaagagaaa atcttgaatt
     7441 cccaatgcga agacactgga tttctttaac ttcttctgaa gtaaaaaatc tagttgaaga
     7501 atctttggat aatgtggggc ttcttcatgc catcaatatg atgccttctg aattatcagg
     7561 aggtatgcgt aaacgaattg gtattgctcg cactttgatt atgaaaccaa aactcattct
     7621 ttatgatgaa cctacaacag gtctcgaccc gataacaagt agagaaattg tacaactcat
     7681 gctcgacctc caaaaaaagt atcatacttc atcagtaatc atttcgca
//
LOCUS       DAORQT010000061         7686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1414_length_7686_cov_7.309265, whole genome shotgun sequence.
ACCESSION   DAORQT010000061 DAORQT010000000
VERSION     DAORQT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_7686_cov_7.309265"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1001
                     /locus_tag="PK134_03535"
     CDS             24..1001
                     /locus_tag="PK134_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRB37951.1"
                     /translation="MSLSLDQIKKPISLEMDCFENKFRDAMRSKVSLVDKIMHYIVKR
                     KGKQMRPMFVFLSAGVCGKINESTYTAASLIELLHTATLVHDDVVDDALQRRGFFSIN
                     AVWKNKIAVLVGDYLLSRGLLIAVQNKEFKLLEIVSNAVRDMSEGELLQMEKARRLDI
                     DEEVYYKIIRQKTASLIASCCSCGAASVQAGDEKVELMRELGETIGLAFQIKDDLFDF
                     EKSISTGKPSGIDIRERKLTLPIIYALSQATSSEKRTIINLIKNEHENESKVAEVLAF
                     VNRQKGIDYAREKMFELRSHAFEIINSFPDSDYRKSLHDLITFTTDRNY"
     gene            1005..1997
                     /locus_tag="PK134_03540"
     CDS             1005..1997
                     /locus_tag="PK134_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37952.1"
                     /translation="MLRKVIVVSLICAGSLFLLLYFTSLFSISELIYPNRITVKSLTA
                     EFHNLKKDELVIDKDVIVSPSQLNLNYRNFDVAIHDHFTLHGWYISGEQPATTTVLLL
                     HDWNESKLSMIKTAEALYQLGFNVCVVDLPAHGESDGEKFIIDQSMNHCLEVVADSLY
                     CLYETDNIAVVAYGLSGIIAASWIKVDSRSHCMILVDPVPGMIAKINELVKEKWNGFS
                     MLVTPFAQIKYRQKTGIDADSLNLQEYLGHLRTPLLVAISDSATDLEIIQSVRIFEEA
                     KSPNKKIWTNQTRSFIRIDTDAEKNMYRAMAAFINSNISSEKPKIKVRKRIAAL"
     gene            1994..3940
                     /gene="dnaG"
                     /locus_tag="PK134_03545"
     CDS             1994..3940
                     /gene="dnaG"
                     /locus_tag="PK134_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRB37953.1"
                     /translation="MIPKETVELIIETSRIEEVVGDFVSLKKRGANLLGLCPFHNEKT
                     PSFTVSPAKGIYKCFGCGKAGDSVRFMMEHEAFTYPEALRYLAEKYKITIEETIPTAE
                     EIQKQDERESLFVLYSFAQKFFSEKLFNTEEGRAIGLSYFNERGFNEAVIEKFQLGYA
                     PDQWDALSQAVIENKFNRQLYEKSGLAVVKEKEQDGQKVYDRFRGRVIFPIHNLSGRV
                     IAFGARQLVNDPKSPKYINSPETDIYHKSKSLYGISFARKAIIQHDECFLVEGYTDVV
                     SLFQAGVENVVASSGTSLTPEQIRLIARFTGNITILYDGDPAGIKASLRGIDLILEEG
                     LNVKVVLFPDGDDPDSFARKNSTTQLMEYISKNAVDFIGFKTGLLLKEVANDPVRKAE
                     LIRDIVESISRIPDGIKRSLYTSQCAAQMDVPEQVLITELNKIRRNLYKKDNEHQQAE
                     LVEKLIEMEYLPQQTILTETNAWYQEQEIIRLLLNYGDKKFNFQGSEKPTTVREFIIE
                     QILPDGFGFDDEIFAGVFSHLTSMSTTDFLSMLKTENSSELEKLRSKAHELLMNKYEL
                     SDWESKGIYVKTEENNFNEAIVNPINHIKLKRVQILKRKNSLLLKEEASLSDERLVEI
                     LSQQKIYEEYIYAFSKLLGIVTLE"
     gene            3950..4474
                     /locus_tag="PK134_03550"
     CDS             3950..4474
                     /locus_tag="PK134_03550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRB37954.1"
                     /translation="MITLIAGTNNKNSHTEKLARIYYQAFLDAKVEVNYLSLTLLNQY
                     IINEDMYDKNDSKINQIGDKYFLNSDKLLFVVPEYNGSFPGILKLLIDAMDVKKYLYF
                     KKAAVVGVATGRAGNLRGIDHLTSILQHTKITVMPHILPVSSVHLEIDEAHEFKNEKT
                     KMALQQHLKQFLAF"
     gene            complement(5141..6244)
                     /locus_tag="PK134_03555"
     CDS             complement(5141..6244)
                     /locus_tag="PK134_03555"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37955.1"
                     /translation="MKIGSYIFELLHQHDCVIVPQFGGFVARYKNATIHPVQHLFSPP
                     SKIVAFNSELTTNDGLLANYISQKLLLSYSDSCKLINDFVTLCKAQLEANQRIQIEKI
                     GSLFFDPEKNLQFIPADNENFLTDSFGLSTVQSPAIQRDRPFEIFSNRDAAVQPAPKR
                     TNHSKLILKIAAYTGVAALIAFAYFNPAINSKISKGLATIFPVTTEIDSQANHSSVNN
                     NQEQKSKEDLIYSESPVSEKNIYETEKPTVDSSASQITAPTPEKEIAAVEEEPKPEPI
                     VETKKIAETVPAVSNATRQYHIVGGCFSVYDNALNLDAELKSKGFSSALIGKNKNGLE
                     IVAIASVATKSEAEEALQQAQNSGYKDAWIMRK"
     gene            complement(6277..7323)
                     /locus_tag="PK134_03560"
     CDS             complement(6277..7323)
                     /locus_tag="PK134_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37956.1"
                     /translation="MKTTKQVLITFDYELFLGNRSGSVSDCLINPTNHVLDVIEQYGC
                     RAIFFVDTTYLLTLKKNFQIAACKKDFETVMNHITEIASRGHEIFPHLHPHWLDAKYL
                     SATNEWQLLNTDKYRLYHINESERSEIFDQSIEILQQRISKVNPSYLVDSYRAGGWCI
                     QPFNTFLPYFIKHNIKFDFSVLGGFYLFSNAQYFDFSKAPQKPIYQFENDITVEQNNG
                     CFTEFNISSIYIPQQIKLFEKLFLKLYYKITGDHSFSRGEGQIVVKIDKALATPQQEG
                     HDILDSRWERIAIELMSAVKQGEYKKYLDANNYMHFISHPKMLTRHNIRMFDKFLKFA
                     SDKYNLESDFRKMV"
     gene            complement(7345..>7686)
                     /locus_tag="PK134_03565"
     CDS             complement(7345..>7686)
                     /locus_tag="PK134_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB37957.1"
                     /translation="SRTEVKRELDNCHSFVLSSNYETFGVVLIESLACGRPVVTTESG
                     GPSDFIHSRNGITSSTSIDSLANAMTKMMEEYNTFDQRALSEECRDRFAEKKIEAEIE
                     ALYLTAINKHK"
BASE COUNT         2471 a         1222 c         1490 g         2503 t
ORIGIN      
        1 acataccttt gcagccgttt attatgtcgc tcagtttaga tcagattaaa aagcccattt
       61 cgctcgaaat ggactgcttt gaaaacaagt ttcgcgatgc catgcgcagc aaagtgtcgt
      121 tggttgacaa aatcatgcac tacattgtta agcgcaaagg caagcaaatg cgacccatgt
      181 ttgtttttct ttcagccggt gtatgcggaa aaataaatga atcaacatac acagcagcat
      241 ctcttattga attattgcac actgcaactt tggtgcatga cgatgttgtt gatgatgctt
      301 tacagcgcag aggttttttt tctattaacg cagtctggaa aaataaaatt gcagtgctgg
      361 taggcgatta tttattgtca cgtgggttac ttattgctgt tcagaataag gagttcaaac
      421 ttttggaaat tgtgagtaat gcagtgcgtg atatgagtga aggtgaactg ctgcaaatgg
      481 aaaaggcaag aagactcgat attgatgagg aagtttacta taagattatt cgtcagaaaa
      541 cagcatcatt aattgcttct tgttgtagtt gtggtgcagc atctgtacag gcaggtgatg
      601 aaaaggttga acttatgcgc gaattgggag agacaatagg gttagctttt cagattaaag
      661 acgacctttt tgattttgaa aaaagtatct ctacaggtaa accttcaggc attgatattc
      721 gtgaaagaaa acttacactg ccgattattt atgcattgtc acaagctact tcctcagaaa
      781 aaagaacaat catcaacctg attaaaaatg aacatgagaa tgaaagtaag gttgctgaag
      841 ttttggcatt cgtcaatagg caaaaaggta ttgattatgc aagagaaaaa atgtttgaac
      901 tcaggagtca tgcttttgaa attatcaata gttttcctga tagtgattac cgtaagtcgt
      961 tgcatgatct gattacgttt actacagatc gcaattacta ggtgatgtta aggaaagtga
     1021 ttgttgtaag tttaatttgt gccggcagtt tatttttact gctatatttc acatcattat
     1081 tcagcatcag cgaattaatt tacccaaaca ggataacggt taaatcatta actgctgaat
     1141 ttcacaatct gaaaaaggat gaattggtta tagataaaga tgtaatagtt tctccttcac
     1201 agctgaatct taactatcgg aattttgacg tggcgattca tgatcatttt acattacatg
     1261 gttggtatat ttcaggcgaa cagcctgcaa ccacaactgt actgttgttg catgattgga
     1321 atgaaagcaa gttgagtatg ataaaaacag cggaagcctt gtatcagctt ggatttaatg
     1381 tatgcgtagt tgatttacca gcacatggtg agagcgatgg tgaaaaattt atcatagatc
     1441 aatcaatgaa ccattgtctg gaagtggtag ctgacagttt gtattgtttg tacgaaacag
     1501 acaacattgc agtggttgct tatgggttgt caggcattat agcagcatca tggattaaag
     1561 tggatagtcg cagtcattgc atgattcttg tagatcctgt accgggcatg atagctaaaa
     1621 tcaatgagtt ggttaaagaa aagtggaatg gtttttcgat gttagtaact ccatttgcac
     1681 aaattaagta ccgtcagaag accggtatag atgcagattc attaaatttg caagagtatt
     1741 taggacattt aagaactccg cttttagtgg ctatttctga tagtgctaca gatttagaaa
     1801 ttattcagtc ggttcgtatt tttgaggaag caaaatcacc gaacaaaaaa atttggacca
     1861 atcaaacaag aagtttcatc aggatagata cagatgcaga gaagaatatg tatagagcaa
     1921 tggcagcatt tattaactca aatatttcat ccgaaaagcc caaaataaaa gttagaaaaa
     1981 gaattgccgc attatgatac caaaggaaac cgttgagttg attattgaga cttccagaat
     2041 tgaggaggtg gtaggcgatt ttgtttcatt aaaaaaacgt ggtgctaact tacttggatt
     2101 atgtcctttc cataatgaga aaacaccatc tttcacggtg tcaccggcaa agggaatata
     2161 caaatgcttt ggttgtggaa aagcaggtga ttcggtgcgg tttatgatgg agcatgaggc
     2221 ttttacttat cccgaagctt tacgttatct ggcagagaag tataaaatta ctatagaaga
     2281 aacaatccct actgctgaag aaattcaaaa acaagatgaa agagaaagtt tatttgtctt
     2341 gtattctttc gcacagaaat ttttttcaga aaaactattc aatacagaag aggggagagc
     2401 aataggcctt tcctatttta atgaacgtgg ctttaatgaa gcagtaattg aaaaatttca
     2461 gttgggttat gctcctgatc aatgggatgc tttgtcgcag gctgttatag agaataagtt
     2521 caacagacaa ttgtatgaga agagtggact tgcagttgtc aaagagaagg aacaggatgg
     2581 tcagaaagtt tatgatcgtt ttcggggaag ggtaattttc ccaattcaca atctttcagg
     2641 tcgtgtaatt gcatttggtg ctaggcaatt ggtaaatgat cccaaaagtc ctaagtacat
     2701 taattcaccc gagacagata tttatcacaa gagcaaatcg ctttatggaa tttcatttgc
     2761 tcgtaaagca attattcaac atgatgagtg ctttctggta gagggatata cagatgtggt
     2821 ttctttattt caggcgggcg ttgaaaatgt tgttgcttct tcaggtactt cattaactcc
     2881 tgaacaaata agacttattg ccaggtttac cggcaacata acaattttgt atgatggtga
     2941 ccctgcagga attaaagcat ccttgcgtgg tattgacctt attcttgaag aagggcttaa
     3001 tgtaaaagtt gttttatttc cggatggaga tgatcctgat tcatttgccc gaaaaaacag
     3061 caccacacaa ttgatggaat atatcagcaa aaatgctgtt gattttattg gatttaaaac
     3121 tggtctgctt ttaaaagaag ttgctaatga tcctgttcgt aaggcagaac tcatccgcga
     3181 tattgtagaa agcatttcac gaattcctga tggtattaaa agaagtcttt ataccagcca
     3241 gtgtgcagca cagatggatg ttccggaaca agttctaatt actgagctga ataaaatcag
     3301 gaggaactta tacaagaaag ataatgaaca tcagcaggca gaattggttg agaagttaat
     3361 tgaaatggag tacctgccgc agcaaaccat tttaactgaa acaaatgcct ggtatcagga
     3421 acaggaaatt attcgcttac tgttgaatta tggagataag aaatttaatt ttcagggtag
     3481 cgaaaagcca actacggttc gtgagtttat aatagaacaa attttacctg atggttttgg
     3541 ttttgatgac gaaatatttg caggtgtttt cagtcatttg acttctatga gtacaacaga
     3601 ttttttatct atgctgaaga ctgaaaatag cagtgaattg gaaaaacttc gttcaaaggc
     3661 acacgaatta ctgatgaata aatacgaatt aagtgattgg gaatcgaaag gtatatatgt
     3721 taagacggaa gaaaataatt ttaacgaggc cattgtgaat ccgataaatc atatcaagtt
     3781 aaaacgtgtg cagatactaa aaagaaaaaa cagtttgctc ctgaaagaag aagcatcttt
     3841 aagtgatgaa agattagttg aaatattatc acagcagaaa atttacgaag agtatattta
     3901 tgcgttttct aaattgcttg gaatcgttac acttgaataa ttaagcaaaa tgataaccct
     3961 aattgcagga acaaataata agaacagtca cactgagaaa ttagcccgaa tttattatca
     4021 ggcatttttg gatgctaaag tcgaagttaa ttacctgtca cttacattgc tcaatcaata
     4081 catcattaat gaagatatgt atgataagaa cgacagtaag attaatcaga taggtgataa
     4141 atatttttta aattcagata aattactttt tgttgtgcct gagtataatg gaagttttcc
     4201 gggtattctc aagctattaa ttgatgctat ggatgtcaag aaatatcttt acttcaaaaa
     4261 agcagcagta gtaggtgtag ctacaggccg tgcaggtaac ctaaggggaa ttgatcatct
     4321 tacttctatt ttgcaacata caaaaatcac tgtaatgcct catattttgc ctgtttcttc
     4381 agtacatctc gaaatagatg aagcacatga gttcaagaac gaaaaaacaa aaatggcact
     4441 gcagcagcat ttgaaacagt ttttggcatt ttaaggcata taagcaaggt ggtttgtgtc
     4501 tatagtgcat ttcatcctga aaatagacaa aatttgatgc attatttgca tttcatccta
     4561 aaagttttgt agataattat catcaggtat aatattgtat caaccttttg aaagtagaga
     4621 attgaataaa tcgccagata ataaccccgg gttttatcag ccgggttact aaaactgaat
     4681 tatgaaagcc gacctaaaaa taagtctata taaagaggcc atcagccccg gtttgccggg
     4741 gcaatggtat ttgctaatga gaatgttaat cctttaatat aattatctgg tatatttttt
     4801 ggttttattg aattaagaat tgttcccatg agatatttat tttctttagt tcgagcattt
     4861 gcctttctgt tgattatcgt atttatgcta attttagcct ttttcaaaaa gattttcagc
     4921 gtgtttcctg tgtctaatag gattcggttt cagaataaaa gaattgatgg caaccttaat
     4981 ttacttatgt attataaaaa tcacaattct tggttggtaa attaatgata attttatggc
     5041 tgcagccaaa acatcaactt ttgtctcgta aagttgttgg gattaaaact taaacacaag
     5101 ccagatttgt tcttaaataa aagaacttta ttccactaat ttatttccgc ataatccagg
     5161 catctttgta acctgaattc tgtgcctgtt gcaaagcttc ctcagcttca cttttagttg
     5221 caacagatgc aatagcaact atctccaaac catttttatt cttgccaatt agcgcagacg
     5281 aaaacccttt tgatttaagt tcagcatcta aattcaatgc attatcataa actgaaaaac
     5341 atccgccaac aatatgatat tgtcttgtag catttgaaac tgcaggtacg gtttcagcta
     5401 ttttttttgt ttctacaata ggttcaggct taggttcttc ttcaacagca gcaatttctt
     5461 tttcaggtgt aggagcagtt atctgacttg cacttgaatc tactgttggt ttttcggttt
     5521 cgtaaatgtt tttttcgctt acaggtgatt cagaataaat taaatcctct ttggattttt
     5581 gctcttggtt gttgttaaca gaagaatgat ttgcttgtga gtcaatttca gttgtaacag
     5641 ggaaaattgt tgctaaacct ttacttattt ttgagttgat agccggatta aaataggcaa
     5701 atgcaattaa tgcagccact cctgtgtatg cagcaatttt taaaatcaac ttagagtgat
     5761 ttgttcgctt tggtgccggt tgaactgctg catctctatt gctgaaaatt tcaaatggcc
     5821 tgtcgcgctg aatggcaggt gactgaacag tacttaatcc aaatgaatcg gtcaaaaagt
     5881 tttcattgtc agccgggata aactgcaagt ttttttccgg atcaaaaaac aaagacccga
     5941 ttttttcaat ttgaatacgt tggttggctt caagctgcgc tttacataaa gtcacaaaat
     6001 cattaatcag cttacaggaa tcactgtacg acagcaataa tttctgactg atataatttg
     6061 ccagaagacc atcgtttgta gttaattcgc tgttgaatgc aactattttt gatggaggag
     6121 aaaataaatg ttgaaccgga tgaatcgttg catttttata acgcgcaaca aagcctccga
     6181 attgtggaac tatcacacaa tcatgctgat gcagaagttc aaatatataa ctgccgattt
     6241 tcatttatca ggcaaatata taaaaaactt tatgccttag accatttttc tgaaatctga
     6301 ctctaaatta tacttgtctg atgcaaactt taggaacttg tcaaacattc tgatattgtg
     6361 tcgggtaagc atttttgggt gagagataaa gtgcatgtaa ttgttagcat ctaaatactt
     6421 tttatattca ccttgtttga ctgcagacat cagttctatt gcaatacgtt cccatcttga
     6481 gtcaagtata tcgtgacctt cttgttgcgg tgtagctaaa gctttatcaa tttttacgac
     6541 tatctgccct tcaccgcgag aaaaactatg gtcacccgta atcttgtaat ataatttcag
     6601 gaaaagtttt tcaaataact ttatttgttg tggaatataa atggacgaaa tattgaactc
     6661 agtaaaacaa ccattgtttt gttctactgt aatatcattt tcaaattgat agataggttt
     6721 ttgtggtgct tttgaaaagt caaaatattg tgcattgcta aacagataaa aaccacctaa
     6781 tacactaaag tcaaacttga tattatgttt aataaaatac ggtaaaaaag tattgaatgg
     6841 ttgaatgcac catccgcctg cacgataact atctactaaa taagatggat taacctttga
     6901 aatacgctgt tgaagtattt cgatagattg atcaaaaatt tcagaacgct cagattcgtt
     6961 tatatgatat aagcggtatt tatcagtgtt taacaattgc cactcattgg tagctgaaag
     7021 atattttgca tctagccagt gtgggtgtag gtgaggaaat atttcatgac cacgacttgc
     7081 aatttccgta atgtgattca ttacagtttc aaaatctttc ttacaagctg caatttgaaa
     7141 attcttcttt agtgtaagaa gataagttgt gtcaacaaaa aaaatggctc tgcatccata
     7201 ttgctcaatt acatcaagta cgtgattggt tggattaata agacaatctg aaaccgatcc
     7261 gcttctgttg cctaaaaata actcataatc aaaagtaatt aatacctgct tggtggtttt
     7321 catattttta agatcagaat tactttactt atgtttatta atagctgtca gataaagtgc
     7381 ttctatttct gcttcaatct ttttttctgc aaatctgtct cgacattctt cagataatgc
     7441 acgttgatca aaagtattgt attcttccat catttttgtc attgcatttg caagactatc
     7501 aattgatgtt gatgaggtga ttccatttct gctatgtata aaatcactcg gtccgcctga
     7561 ttctgttgtt accaccggtc ttccacaagc taaactctct atgagtacaa caccaaatgt
     7621 ctcatagtta cttgaaagca caaacgaatg gcaattgtct aattctcttt ttacttcagt
     7681 ccgtga
//
LOCUS       DAORQT010000062         7608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1441_length_7608_cov_9.816100, whole genome shotgun sequence.
ACCESSION   DAORQT010000062 DAORQT010000000
VERSION     DAORQT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_7608_cov_9.816100"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK134_03570"
     CDS             <1..467
                     /locus_tag="PK134_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB37958.1"
                     /translation="VVVDKPVEEEQPPPQEKLSETNVGVVTQEGDPNATELPPEPVVA
                     DPSEGKIFTIVEEMPQFPGGGEAALIKYLQNNIKYPAMARENGIEGIVYVTFVVDKDG
                     KVKDAKILRGKGAGLDDEALRVVRAMPDWKPGKQNGRSVAVQYNLPVNFKLQ"
     gene            480..692
                     /locus_tag="PK134_03575"
     CDS             480..692
                     /locus_tag="PK134_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37959.1"
                     /translation="MIRKVGLYFNLLMIAGYAVMGFFLIFADNLTFINQPQQQWLGGI
                     LIVYAIYRGYRSYRSSQKEKEPLDES"
     gene            682..1632
                     /locus_tag="PK134_03580"
     CDS             682..1632
                     /locus_tag="PK134_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRB37960.1"
                     /translation="MKVKNIFLLLLSTAITLQLVSCGGDAFKPDLSDTPTSGNLKIVS
                     DESFEPLMNSQAETFMSLYKNAKIEISYKTEADAINYLMNDSVKVIIAGKDLTPEQHQ
                     YFKDHKVLARSTKIAVDALALIINKENIDSLLTLSQFESIITGKTNNWNAINNKNNLG
                     EVAVVFDKSGSSNVRFLKEKFLHNGNFPANCYALNSNKEVMDYVINKKNAIGIIGVSW
                     ISDSNDSTAMTFLNQIKVVALKNDTSTEYTDYYQPYQAYIALKQYPLTRDVFLINRQS
                     RNGLGTGFASFVAGDQGQRLVRLKGLLPATMPVRIVRTGN"
     gene            1666..3252
                     /locus_tag="PK134_03585"
     CDS             1666..3252
                     /locus_tag="PK134_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB37961.1"
                     /translation="MKKTVFFLSLMFAQAVHAQTLKEAIAMTDNEQYEISGDMFKSLL
                     QKEPANGTNWFYYGENYFKSDNLDSAAIAYSKGLQAEPGNPINLVGTGKIKLEKGLNS
                     EARADFDKALTMSGSKTSLVQSEVAESFIRTKNKDLSYAIKLLNNAIAVDSKNAELYI
                     LLGDAYSEQNNGTLAAENYNRALDLDKKAVKAIVQKGVLYKRSTNYEGAAAEFQSAIK
                     LNPDFAPSYRELAETYFKMRKLDEAKQSYRKYLELSKNNSMARMRYASFLFMSGDYAD
                     AQTEINQLSSIDSSNIGMLRLSSYNAFEMNDIPKAKMLMEKIMNTTIENQRTAMDYEY
                     NGKILLKEGNDSLAAENFEKSYQIDTTKTEILQDMGSMYMKLKKYSEAVNAYERRVNN
                     GKGLTSTDYFNLARAYYFNKDYTHADTMFAKVIELLPTWPNGILWRAQTNAQLDPDSK
                     EGKAKPYYEKYIEVALTDTANSDKYKTGLIESYKYLGSYYYLIDKKPDECRSYWKKVL
                     EIAPDDKQAKQVMDGLNQKK"
     gene            complement(3328..5043)
                     /locus_tag="PK134_03590"
     CDS             complement(3328..5043)
                     /locus_tag="PK134_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRB37962.1"
                     /translation="MFRFLLLFLFLDNVCLSQDVTDYYLHNRTPQWHEVIEYYKKLDK
                     QFPQAKLFTEGTTDVGKPLHLFVISSDGDFNPVSLHQKKRLIWLINNGIHPGEPDGID
                     ASMIFAKNVLSKISSEEYKNVVICIIPIYNIDGALNRGCCSRVNQDGPLEYGFRGNYQ
                     NLDLNRDFVKCDTKNTESFIRIFQKWNPDLFLDTHVSNGADYPYNITLIATQKDKLTP
                     PLGKYLSEKMLPELYRNMESKSEKMIPYVNTIKDTPDSGLEGFLETPRYASGYTALFN
                     TIGFISETHMLKPYSVRVKATITFMETLRQYAARNAAEILQKRVEAINFTKATHEFPV
                     QWQLDTLSKDSIRFDGYTAVYSTSKITGLQRLSYDKSRPYSKFIPYQNNFKPQVVIKQ
                     PFAYIIPQCWSHVIERLQLNGVEMQTITADTIIEVECYKIGQYKTTSKPYEGHYLHSN
                     VTVEKQVRKVKFFKGDRLIKVNQLANRYIVETLEPQGTDAFFAWGFFDGILQQKEWFS
                     DYVFEDIAQKMIQENPALMDSLNDAIKSDSTLKNNHYMQLNMLYRLSPYFEKSYMIYP
                     VYRLM"
     gene            complement(5049..5972)
                     /locus_tag="PK134_03595"
     CDS             complement(5049..5972)
                     /locus_tag="PK134_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRB37963.1"
                     /translation="MKKFTIAIHGGAGTISPSTMTPQKETAYKQALQTALTAGYKVLE
                     ARGSALDAVTQAVIELENTPLFNAGRGSVFTYEGKHEMDASVMNGKDGMAGAVAGVRN
                     VMNPILLAKAVMQHTDFVFLHGNGAEELARAQQLTFCDDQYFFDDFRYQQWQEVKGSD
                     ATKLDHSSEKKFGTVGAVAKDLYGNLAAATSTGGMTNKRYARIGDTPIIGAGTYANYN
                     ICAISCTGHGEYFIRSVVAYDIHCLMEYKGLSLKEACQLVVNDKLVKTGGEGGLIAVD
                     SSGTPQLVFNSEGMYRGYFSSDEQPVTEIYR"
     gene            complement(5985..6794)
                     /gene="murI"
                     /locus_tag="PK134_03600"
     CDS             complement(5985..6794)
                     /gene="murI"
                     /locus_tag="PK134_03600"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRB37964.1"
                     /translation="MTVNQPIGIFDSGIGGLTVAAAIGRLLPQEQLIYFGDTAHVPYG
                     DKSVELIGSYAERITEFLLHEQKCKAIVIACNTASAAAYSLLRDRYKGSVPVINVIDP
                     MIEAVIADDDIISVGIIATKTTIASGTYQEKFSRRKPQLLFHPLATPLLASMIEEGFY
                     NDNISSAVLEEYLSYEPFKSIDGLVLACTHYPLIKKEINHFFQSKVKLFDSAEVVASK
                     LKKILEAESLISLERKKTNRFYVSDFTQSFEHSTKIFFGKQVNLELMNLWK"
     gene            complement(6791..>7608)
                     /locus_tag="PK134_03605"
     CDS             complement(6791..>7608)
                     /locus_tag="PK134_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRB37965.1"
                     /translation="HKLDIFEYERPIGIQIFGSDVESMRQAAIIAEQAHPDLIDINYG
                     CPVKQVACKGAGAALLQDIPKMVSMTKAIVDAVKLPVTVKTRLGWDDKTKNILEVAER
                     LQDIGIKALTVHARTRSQMYKGSADWGLVGELKNNPRLTIPVFGNGDVDSPQKALEMK
                     NRYGVDGIMIGRASIGYPWIFNQIKEYLAGNCAIAVPGIAERVEVCRTHLIKSVEWKG
                     TIVGILEMRRHYTNYFKGMDHFRDFRVKLVTSNSLNELLDLLEQIKIKYKEAA"
BASE COUNT         2489 a         1458 c         1338 g         2323 t
ORIGIN      
        1 cggtagtagt tgacaaacct gttgaggaag agcaaccacc accacaagaa aaactttcag
       61 aaacgaatgt tggtgttgtt acgcaagaag gtgatccaaa tgcaacagaa ttaccaccgg
      121 aacccgttgt ggccgatcca agcgaaggta aaatttttac catcgttgaa gagatgccgc
      181 aattccccgg aggaggcgaa gctgcactga taaaatatct tcagaacaat attaaatatc
      241 ctgctatggc acgtgagaat ggtattgaag gtattgtgta tgttacattc gtggttgata
      301 aagatggcaa ggttaaggac gctaaaatac ttcgcggtaa aggtgcaggc ttagacgatg
      361 aagctttacg tgtagtacgt gctatgccgg actggaaacc cggtaaacaa aatggaagga
      421 gcgtagcagt acagtataac ctaccggtaa actttaaatt gcaataactc tttgtcggtg
      481 tgatccgcaa agttggtcta tactttaatt tactcatgat tgcaggttat gcagtcatgg
      541 gttttttttt gatatttgca gataatctta cttttattaa tcagccacaa caacaatggt
      601 tgggcggaat attaattgtg tatgcaattt atcgtggcta cagaagctat cgttcaagtc
      661 aaaaagaaaa agaaccactt gatgaaagtt aaaaatattt ttctcctact cctatcaact
      721 gcaattacgc tgcaattagt ttcctgtggt ggagatgcct ttaaacccga tttgagtgat
      781 acgcccacat caggaaattt aaaaatagtt tctgacgaaa gtttcgagcc tctcatgaac
      841 agtcaggctg aaacgtttat gtcactctat aaaaatgcta aaattgaaat aagctataag
      901 acagaagcag atgcaattaa ttatctgatg aacgactcgg tgaaagttat tattgcagga
      961 aaagacctta ctccggaaca acatcaatat tttaaagatc ataaagtgct ggcacgctca
     1021 actaaaattg ctgttgatgc ccttgcatta attatcaata aagaaaacat agattcactg
     1081 ctgacattat cacaatttga atccataata acaggaaaaa caaataactg gaacgctata
     1141 aataataaaa ataacttggg tgaagtagct gtagtgtttg acaagagtgg ctcaagtaat
     1201 gtacgttttc tgaaagaaaa atttttacac aatggaaatt ttcctgccaa ttgttatgca
     1261 ctcaactcaa acaaagaggt tatggattac gttattaata aaaagaatgc aattggtatt
     1321 attggtgtta gttggataag cgacagcaat gattccactg caatgacttt tctgaatcag
     1381 attaaagttg ttgcacttaa aaatgataca tcaacagaat atacagatta ttatcaacct
     1441 tatcaggcat atatagcatt gaagcaatat ccgttaacca gagatgtatt tttgattaac
     1501 cgccagtcaa gaaacggact tggcaccggc tttgcatcat ttgttgcagg cgatcagggg
     1561 cagcgtcttg taagattaaa aggactgctg ccagctacga tgccggttag aattgtaaga
     1621 acaggaaatt gacagaacta aattataatt accaatttta atagaatgaa aaaaacagtt
     1681 ttttttctaa gcctgatgtt cgcacaggct gtacatgcac aaacacttaa agaggctatt
     1741 gcaatgacag ataatgagca atatgaaatt tccggtgaca tgtttaaatc attattgcag
     1801 aaagagcctg ccaatggcac caattggttt tattatggcg aaaattattt taaaagtgac
     1861 aacttagatt ctgctgctat tgcctactca aaaggattgc aggcagaacc cggcaatcca
     1921 ataaatttag tagggacagg aaaaataaaa ttagagaaag gtcttaacag tgaagcacgt
     1981 gccgatttcg ataaagcatt aacaatgtca ggctctaaaa catcgttggt gcaaagcgaa
     2041 gtcgcagaaa gttttatcag aacaaaaaat aaagacctta gctacgcaat taaattacta
     2101 aacaatgcca ttgccgttga ttcaaaaaat gctgaattgt acatcctttt aggcgatgcc
     2161 tattccgaac aaaacaatgg cacattggct gcagaaaatt acaaccgtgc attggactta
     2221 gataaaaaag ctgtaaaagc tattgttcaa aaaggggtat tgtataaacg ctctacaaac
     2281 tatgaaggtg ctgcagcaga atttcaatca gccataaaac tgaacccgga ttttgcacca
     2341 tcttatcgcg aacttgctga aacttatttc aaaatgcgca agctggatga ggcaaaacaa
     2401 tcctacagaa aatatttaga gttgagtaag aataacagta tggcaagaat gcgttatgca
     2461 tcctttctgt ttatgagtgg tgattatgca gatgcacaaa cggaaattaa tcaactgtct
     2521 tcaattgatt catcgaatat tggtatgctt cgtctgtcct catataatgc atttgagatg
     2581 aatgatattc caaaagcaaa aatgctgatg gaaaaaatca tgaacactac tatagaaaac
     2641 caacgcactg caatggacta tgaatacaat ggaaaaatat tacttaaaga aggtaatgac
     2701 tcattagctg ctgaaaattt tgaaaagtca tatcagattg acactaccaa gacagaaatt
     2761 cttcaggaca tgggtagtat gtatatgaaa ttaaaaaaat acagcgaagc tgttaatgcc
     2821 tacgagcgaa gagtaaacaa tggtaaaggc ttaacttcta ccgactactt taaccttgca
     2881 agagcatatt attttaacaa agactataca catgcagata ccatgtttgc aaaagttatt
     2941 gaactgttgc caacatggcc taatggtatt ttatggcgcg cacaaaccaa tgcgcaactc
     3001 gaccccgact ctaaagaagg taaagcaaaa ccttactatg agaaatacat tgaagtagct
     3061 ctaacagata ctgccaattc agataaatat aaaaccggac ttattgagtc gtataaatat
     3121 cttggtagtt actattatct gatagataaa aaacctgatg agtgcagatc atactggaaa
     3181 aaagtacttg aaattgctcc tgacgataaa caggcaaagc aggttatgga cggattgaat
     3241 cagaagaagt aagttatttc gataataaca tttggccacc aatcgaacag taaatgtgaa
     3301 tttactgttc gatgtcgttt taaccaatta catcaatcta tacacaggat aaatcatata
     3361 tgatttctca aagtacggtg acagtctgta cagcatattc aactgcatgt aatgattgtt
     3421 ctttaaagtt gaatctgatt taattgcatc attcaaacta tccatcaatg caggattctc
     3481 ctgaatcatc ttttgagcaa tatcttcaaa aacataatcg ctaaaccatt ctttttgttg
     3541 taagatacca tcaaaaaatc cccatgcaaa aaatgcatct gtgccttgag gttccaaagt
     3601 ttcaacaata taacgattgg caagttgatt aactttaatc aatctatcac ctttaaaaaa
     3661 tttcacctta cggacttgct tttccactgt aacattcgaa tgcagataat gtccttcata
     3721 aggttttgaa gtagttttat attgaccgat tttataacac tcaacttcta tgatagtatc
     3781 tgctgttatg gtttgcattt ctacaccatt caactgcaac ctttcaatca catggctcca
     3841 acattgtgga atgatatagg caaaaggttg tttaattact acttgcggtt tgaaattatt
     3901 ctgatatgga ataaactttg agtaaggtct gcttttatca taactcagtc tttgaagccc
     3961 tgtaatttta cttgtactgt aaacagcagt gtagccatca aaacggatac tgtcttttga
     4021 aagtgtatca agttgccatt gaactggaaa ttcatgagtt gcctttgtaa aattaattgc
     4081 ctcaacacgt ttttgtaaaa tttctgctgc attacgggct gcatattgcc gaagtgtttc
     4141 cataaatgta atagttgcct ttactctgac agaatatggc ttgagcatgt gcgtttcaga
     4201 aataaaacca atagtattaa acaatgcagt atagccactg gcatatcgag gtgtttccaa
     4261 aaatccctct aaacctgaat cgggagtgtc cttaattgta ttaacataag gaatcatttt
     4321 ttcagactta ctttccatgt tccgatatag ttccggaagc attttttcag aaagatattt
     4381 tcctaatggc ggtgttagtt tatctttctg tgtggctatt aaagtaatgt tgtaaggata
     4441 gtcggcacca ttacttacat gagtgtctaa aaacaaatca ggattccatt tttgaaaaat
     4501 acgaataaat gattctgtat tcttagtatc gcacttcaca aaatcacgat ttaaatcgag
     4561 gttctgataa ttacctctga aaccatattc caatgggcca tcctgattga cgcgactgca
     4621 acaacctctg ttcagtgcac catcaatgtt atagatagga attatacaaa taacaacatt
     4681 tttatattct tcagaactta tcttactcag cacatttttt gcaaaaatca ttgatgcatc
     4741 aataccatca ggctcaccgg gatgaatacc gttattgatt aaccatatca atctcttttt
     4801 ctgatgtaac gaaacgggat taaagtcgcc atcagaagaa attacaaaca aatgtaatgg
     4861 cttaccgaca tcagtagttc cttctgtaaa aagtttggcc tgagggaact gtttatccaa
     4921 cttcttataa tattcaatta cttcatgcca ttgtggtgta cggttgtgta aataataatc
     4981 tgtaacatct tgtgataaac aaacattgtc taaaaataaa aacaacaata aaaaacgaaa
     5041 catatacatc agcgatatat ttctgttacc ggttgttcat cagaactaaa atatccgcga
     5101 tacatccctt ctgaattaaa tactaattgt ggagtaccgc tactatcaac tgcaatcaaa
     5161 ccaccctccc ctccggtttt aacgagttta tcattcacta ccaactgaca tgcttctttt
     5221 agagaaaggc ctttatattc catcaggcaa tgaatatcat aagccacaac tgaacgaata
     5281 aaatattcac catgacctgt acaactaatt gcacaaatat tgtaattagc ataagtacct
     5341 gcgccaataa ttggggtatc gccaatgcgt gcatatcgtt tattagtcat acctccggta
     5401 gaagtggcag cagcaagatt tccatataaa tcttttgcaa ctgcgcccac tgttccgaat
     5461 ttcttttcac tgctgtgatc aagttttgtt gcatcagacc ctttcacttc ctgccattgc
     5521 tgatacctaa agtcatcaaa aaaatattgg tcatcacaaa atgtcaactg ttgtgccctt
     5581 gcaagttctt ctgcaccgtt gccatgcaaa aaaacaaagt cagtatgctg catcactgct
     5641 ttggcaagta aaataggatt catgacattt cgaactcctg caactgcacc tgccattcca
     5701 tctttaccat tcattacaga tgcatccatt tcatgcttac cttcataggt aaaaacacta
     5761 cctctgcctg cattaaacaa aggcgtgttc tccaattcaa ttacagcttg tgtaacagca
     5821 tctaaggcgc ttcctcttgc ttccaacact ttgtatcctg ctgttaatgc agtttgcaat
     5881 gcttgtttgt atgccgtttc tttctgaggt gtcatggtag atgggctgat agtgcctgca
     5941 ccgccatgaa ttgctattgt aaattttttc atttgctgaa aatattattt ccaaaggttc
     6001 atcaattcaa gattcacctg cttaccaaaa aatattttag tgctatgttc gaatgattgg
     6061 gtgaaatctg aaacataaaa tctattggtc tttttccgtt ctaaactaat taaagattct
     6121 gcctccaata tttttttcaa ttttgaagca actacttcag cagagtcaaa caacttaact
     6181 tttgattgaa aaaaatgatt tatctccttc ttgattagtg gataatgtgt acatgcaagt
     6241 accaatccat caatggactt aaatggctcg tatgagagat attcttccaa tacagcagaa
     6301 gaaatattat cattataaaa tccctcttca atcattgaag caagcaatgg tgtggctaaa
     6361 ggatgaaaaa gtaattgtgg ctttcgtctt gaaaattttt cctgataagt acctgatgca
     6421 atagttgttt tggttgcaat gatacctaca cttattatat catcatcagc aataactgcc
     6481 tcaatcatcg ggtcaatgac attgataacc ggaacacttc ctttatagcg atcgcgcaat
     6541 aaagaatatg ctgcggctga tgctgtgttg caggcaatga ctatggcttt acatttttgt
     6601 tcgtgcaaca aaaattctgt aattctttct gcataactgc caattaactc aaccgattta
     6661 tccccataag gaacgtgagc agtatcgcca aaataaataa gttgttcttg tggcaacagt
     6721 ctgcctattg ctgcagcaac agtaagtccg ccaatgccgg aatcaaaaat acctataggt
     6781 tgattaactg tcatgccgct tccttgtatt ttattttaat ctgctcaagc aaatcaagca
     6841 gttcgtttaa gctgtttgaa gtcaccaatt ttactctgaa atcgcgaaaa tgatccatcc
     6901 ctttaaaata gtttgtatag tgccttctca tttcaagaat accgacaatg gtacctttcc
     6961 attccacaga ctttatcaga tgtgttcggc atacttctac tctttctgca ataccgggta
     7021 cggcaatggc gcagttaccg gcaagatatt ctttaatctg attaaatatc caagggtaac
     7081 caatactggc tctgccaatc attatgccat caactccata tcggtttttc atttccaatg
     7141 ctttctgtgg tgagtcaaca tcaccatttc cgaaaacagg aattgtcagt cgtggattat
     7201 tcttcaactc tccaacaagg ccccagtcgg cactgccttt atacatttgt gagcgtgttc
     7261 gtgcatgaac agttaatgct tttatcccta tatcctgaag ccgttctgca acttcaagaa
     7321 tattttttgt tttatcatcc cagcctaatc ttgtttttac agtaacagga agtttaactg
     7381 catcaacaat tgcctttgtc atactcacca ttttaggaat atcctgcaac aatgctgcac
     7441 ctgcgccttt gcatgctact tgctttaccg ggcatccgta attaatatca atcaaatcgg
     7501 gatgtgcctg ctctgcaatg atggctgcct gtctcatgga ttcaacatca gaaccaaaaa
     7561 tctgaattcc gattggtctt tcatattcaa aaatatcgag cttatgtg
//
LOCUS       DAORQT010000063         7541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1457_length_7541_cov_12.017633, whole genome shotgun sequence.
ACCESSION   DAORQT010000063 DAORQT010000000
VERSION     DAORQT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_7541_cov_12.017633"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2492
                     /locus_tag="PK134_03610"
     CDS             <1..2492
                     /locus_tag="PK134_03610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRB37966.1"
                     /translation="IDVELTSTLRTGMHRYTFNKPGLAYIYLDLNHRDKLTASNINIV
                     YDSTIKVYRKSEGWAKEQILYANIRFSKPFIQSKQLGNKKAVFVFSVSEKESIMAKVA
                     LSAVDDDGAERNMILENPDWNFEHIKINAAQKWNEELSKIKVSSSDKNKLTVFYTAMY
                     HCMMHPNIYNDVDGRYRGRDLQIHQTDHNYYTVFSLWDTFRTWHPLMTIIDSARTVDF
                     IRTFLLQYEQGGLLPVWELAANETECMIGYHAVSVIADAMMKGIKGFDYEKAFEACKF
                     SAEQRNRFGLGAYIDKGFLEAEDEHESVSKTLEYAYDDWCIAQMAKLLGKEDDYNTYM
                     QRSQSWKNLFDPVTGFIRPRSNGGWLNPFDPFEVNNNYTEANGWQYNFFVPQDIDGMI
                     SYNGGKEKFEKKLDQLFSASTKTTGREQSDITGLIGQYAHGNEPSHHIAYLYNYVDKP
                     GKTEKIVNKIINDFYKNSPDGLIGNEDCGQMSAWYVMSALGMYPVCPGSNQYTLTTPQ
                     FDKVEILTSGNKTFTIDVKNKTNKANFLRVKKTKNISFRDGLLFNHNRFSTDNLLVLT
                     ADSIPSILRKKDLADSIYYSDDLFRVPIITASTTVFKDSLKVSIKSFQKDKNIVYLQQ
                     GPFERPAKIDYKKPFYIYDTSVIHAFVTDSSGIESKWAKAEYFKLPHPKWKVSIKGNY
                     NPQYNAGGANGLIDGLHGDINWRKGRWQGYQSQDFECVVDMGNQQEVSSFNTSFLQDT
                     RAWILMPISVEYFTSDDGKLFNSAGIVQHQTAPDNYEISIENFKLQTEALITARYIKV
                     VAKNFRKLPSWHQGFPMGGEAFIFVDEVEVE"
     gene            complement(2501..3028)
                     /locus_tag="PK134_03615"
     CDS             complement(2501..3028)
                     /locus_tag="PK134_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB37967.1"
                     /translation="MATKRIDLKAFRIKSGRNRKKYTTFLKGLHSRKVKQLTAKAKKL
                     NDLAFKKIDCLACGNCCKTMTPTWQPADIKRVAAHFKMTVQQFKDKWLYKDETGDWMN
                     TKTPCQFLQRDNKCSIYEIRPKDCSGFPHLQHTDFLIGREVNIPNMTSCPITYEVVER
                     MYKLVVEKDATVLND"
     gene            complement(3112..4836)
                     /gene="lysS"
                     /locus_tag="PK134_03620"
     CDS             complement(3112..4836)
                     /gene="lysS"
                     /locus_tag="PK134_03620"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRB37968.1"
                     /translation="MALSEQEQIRREKLNELLQMGINPYPAEKFEINTNSTFILENYE
                     QAEARFGAGKEEVSIGGRIMSVRDMGKAAFAQIQDAEGKIQIYIKRDEICKGDDKTLY
                     DTVWKKLIDIGDIIGIKGYVFKTKTGETSIHASSFILLCKSLRPLPIVKEKDGEVFDA
                     VIDPDLRYRQRYVDLIVNPQVKETFRKRTRLVNTMRNYLNEKGYLEVETPILQPLYGG
                     AAARPFKTHHNTLDMTLYLRIANELYLKRLIVGGYDGVYEFSKDFRNEGMSRFHNPEF
                     TQIELYVAYKDYEWMMNLVEEMIEKVALELHGSTAVKVGEKTIDFKRPWKRFTMYEAI
                     EHFTGVDVSEMDEMAMRKAAAQMGIELDDTMARGKIIDEIFGSKCEANLIQPTFITDY
                     PVEMSPLAKKHRSKPGLVERFEAICNGKEMCNAFSELNDPIDQRKRFEEQLELGKRGD
                     EEAMQLDEDFLRAIEYGMPPTAGLGIGIDRLSMIMTNAASIQDVLFFPQMKPETIETN
                     AATDTGLSHIPAPWNDVISRMGITSKQQLKEANPNKLFNDMGGMRKKLKLDISMVTLE
                     EVKSWINV"
     gene            complement(5129..5962)
                     /gene="tsf"
                     /locus_tag="PK134_03625"
     CDS             complement(5129..5962)
                     /gene="tsf"
                     /locus_tag="PK134_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRB37969.1"
                     /translation="MSTVTISASDVNKLRQMTGAGMMDCKKALMEANGDFEKAIDELR
                     KKGQKVAANRADREAKEGYIVAKTNNNATKGYLVALSCETDFVAKNAEFVKFAEDVLE
                     TALTNNAASLDALLALQLQGTTISEKLIEMVGKIGEKIEITRYEVIEAPKVVVYIHPG
                     NRVVSMVGLSSATAANEAGKDVAMQIAAMNPVAIDKDDVDQSTLDREIEIGKEQARAE
                     GKPENMIEKIAMGKLNKFYQESTLLNQAFIKEDKKTIRQYLDGVEKGLTVKAFRRVQI
                     G"
     gene            complement(6005..6874)
                     /gene="rpsB"
                     /locus_tag="PK134_03630"
     CDS             complement(6005..6874)
                     /gene="rpsB"
                     /locus_tag="PK134_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRB37970.1"
                     /translation="MSINTQELLDAGCHFGHLKRKWNPKMAPYIFMERNGIHIIDLNK
                     TAAKLEEATAAIKQIAKSGKKILFVATKKQAKEIVANAAKSVGMPYITERWPGGMLTN
                     FATQRKSVKKMTGFDRFMKDESFNNISKKERLQVSRQKAKMEKLIGSIADLTRLPAAL
                     FIVDVLKEHIAVSEAQKLGIPTIAICDTNTDPTLIDFPVPANDDATKSISLIMNAMIK
                     AIEEGLEERKMDKEADREREAAAEEEGGERGEGEKEFTMSDEGDDSGETKTKSPAKKA
                     TTRPRKGTTTSKR"
     gene            complement(6894..7280)
                     /gene="rpsI"
                     /locus_tag="PK134_03635"
     CDS             complement(6894..7280)
                     /gene="rpsI"
                     /locus_tag="PK134_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRB37971.1"
                     /translation="MNNIHKIGRRKTAVARVYLTPGTGTIVINNREFKNYFTTGPLQN
                     KVTQAFAVINANEQFDVKANVMGGGITGQAEAIRLAISRALCEMNPENRPALKKEGLL
                     TRNPRMVERKKSGQKKARKKFQFSKR"
     gene            complement(7287..>7541)
                     /locus_tag="PK134_03640"
     CDS             complement(7287..>7541)
                     /locus_tag="PK134_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HRB37972.1"
                     /translation="KVKLTGKKMAQKVYVRHTGYPGGHRFTSPEKLMDTYPERILENA
                     IHKMLPKNRLGNALKKNLRVFAGSEHSHEAQNPRKITLDI"
BASE COUNT         2156 a         1472 c         1327 g         2586 t
ORIGIN      
        1 gtattgatgt agaattaaca tcaaccctaa gaacaggaat gcatcgttat acatttaaca
       61 aacccggatt agcttatatc tatcttgatt taaatcatcg tgataagtta acagcaagta
      121 acattaatat tgtttacgat tccactatta aagtttacag aaaaagcgaa ggctgggcaa
      181 aggaacaaat cctatatgcc aacattcgtt tttcaaaacc ttttattcaa tcaaaacaat
      241 taggaaacaa aaaagctgtg ttcgtttttt cagtttctga aaaagaaagt atcatggcta
      301 aggttgcatt atctgctgtt gacgatgatg gtgctgaacg aaatatgatt ctcgaaaatc
      361 ccgactggaa ttttgaacac ataaaaataa atgctgcaca aaaatggaat gaagaactat
      421 cgaaaataaa agtcagcagc agcgataaaa ataagttaac cgttttttat acagcaatgt
      481 atcactgcat gatgcatccc aatatttata acgatgttga tggcagatac cgtggaagag
      541 atttacaaat tcatcagact gaccataatt attatactgt cttttcgttg tgggatacat
      601 tcaggacatg gcatccgttg atgactataa ttgatagtgc acgcacagtt gattttatcc
      661 gtactttttt attgcagtat gagcaaggag gcttacttcc tgtttgggaa ctggcagcta
      721 atgaaacaga atgtatgatt ggctatcatg cagtgagtgt tattgccgat gcaatgatga
      781 aaggcattaa aggttttgac tatgaaaaag catttgaagc atgcaagttt agtgcagaac
      841 aacgcaaccg ttttggatta ggtgcatata ttgacaaagg ttttcttgaa gcggaagatg
      901 agcatgaatc tgtttcaaaa acattagagt atgcttatga tgactggtgc attgcacaaa
      961 tggcaaaact gttgggaaaa gaagatgatt acaacaccta catgcaacgc tcgcagtcat
     1021 ggaaaaattt atttgaccct gtgacgggat ttatccggcc aagaagcaac ggtggctggc
     1081 taaatccttt tgaccctttt gaagtgaaca acaattatac tgaagcaaat ggatggcaat
     1141 acaacttttt tgttcctcag gatattgatg gtatgatttc atacaatggc ggaaaagaaa
     1201 aattcgaaaa aaaattagat cagcttttta gtgcttctac aaaaactaca ggtagagaac
     1261 aatctgatat tacaggatta ataggacagt atgcacacgg taacgaacct agtcatcaca
     1321 ttgcttactt gtacaattat gttgacaagc cgggaaagac tgagaaaatt gtcaataaaa
     1381 taatcaatga tttttacaag aacagtccgg atggtttaat aggcaatgaa gattgcggac
     1441 agatgagtgc atggtatgtg atgagtgctc ttggcatgta ccctgtttgt ccgggaagca
     1501 atcaatatac gctgactact cctcagtttg ataaagttga aattttaact tcaggaaata
     1561 aaacatttac tattgatgta aagaacaaaa caaacaaagc aaatttcctt agagtgaaga
     1621 aaacaaaaaa tatttctttt cgggatggtt tgctttttaa tcacaatcgt tttagcactg
     1681 acaatctttt agtactaact gccgacagta ttccttctat acttcgtaaa aaagatttgg
     1741 ctgatagtat ttactattcc gatgatttat ttcgtgttcc tataattact gcttcaacaa
     1801 cggtatttaa agattctctt aaagtcagta ttaaatcttt tcaaaaagat aaaaacatag
     1861 tttatctgca acaaggacca tttgaacgtc cggcaaagat tgattataaa aaaccatttt
     1921 atatttatga tacgtcagta attcatgctt ttgttacaga ctcaagcggt attgaaagta
     1981 agtgggcaaa agctgagtac tttaaacttc ctcatccaaa atggaaagtt tctattaaag
     2041 gaaattacaa tccacaatat aatgcaggcg gtgcaaacgg attgattgat ggactgcatg
     2101 gtgatataaa ctggcgtaaa ggtcgctggc aaggttatca gtcgcaggat tttgaatgtg
     2161 ttgttgatat gggtaatcag caagaagttt cttcttttaa caccagtttt ttgcaagata
     2221 caagagcctg gattcttatg cctatttcag tggagtactt tacatctgat gatggtaagt
     2281 tatttaatag tgcaggtatt gttcagcatc agactgcacc ggataattat gaaataagta
     2341 ttgagaattt taaattacaa accgaagctc taataacagc acgttacata aaagttgttg
     2401 ctaaaaattt cagaaaacta ccttcatggc atcagggttt tccaatgggt ggcgaagctt
     2461 ttatttttgt tgatgaagta gaagtagaat gataaagtac ttagtcgttc agtaccgttg
     2521 catctttctc tacaactaat ttatacatac gctctacaac ttcataggta atagggcaac
     2581 tggtcatatt tggaatattt acttcgcgac caattaaaaa gtctgtgtgt tgcagatgtg
     2641 gaaatccgga acagtcctta ggacgaattt cataaatact gcacttatta tctctttgta
     2701 aaaactgaca tggtgttttt gtattcatcc agtcgccagt ttcatcttta tacagccact
     2761 tgtctttaaa ctgttgcact gtcattttaa aatgtgctgc aactcttttt atatctgcag
     2821 gttgccatgt tggtgtcata gtcttacaac aattaccaca ggcaagacaa tcaatttttt
     2881 taaaagcaag atcattaagt ttctttgctt ttgctgtcaa ctgctttacc ttgcggctat
     2941 gcagtccttt cagaaaagtt gtgtactttt ttctgtttct cccagattta attcggaatg
     3001 cttttaaatc aattcttttt gttgccatag tttataaaca agcatgcaaa tgaacaaatt
     3061 tattttataa cttaattgat ggtgtattga agatattact tcaggatgct tttaaacatt
     3121 aatccagctt ttcacttctt caagtgtaac catgcttata tcaagtttca gttttttacg
     3181 catgccaccc atgtcgttaa acaatttgtt aggattagct tctttgagct gttgttttga
     3241 agtgattccc attctggaaa taacatcatt ccaaggcgca ggtatgtgcg acaatcctgt
     3301 atccgttgct gcattggttt caatagtttc gggcttcatc tgtgggaaaa acagcacatc
     3361 ctgaatgctg gcagcgtttg tcataatcat actcaaacga tcaattccta ttcctaaacc
     3421 tgctgttggc ggcataccat attcaatggc gcgaagaaaa tcttcgtcaa gttgcattgc
     3481 ttcctcatcg ccacgtttgc caagttcaag ttgttcttca aatcttttac gctgatcaat
     3541 cggatcattc aattcagaaa atgcattgca catttcttta ccattacata ttgcttcaaa
     3601 gcgttcgacc aatccgggtt tgctacgatg cttctttgcc aatggcgaca tttcaacagg
     3661 atagtctgta ataaacgtag gttgaatgag gttggcttcg cacttgcttc caaaaatttc
     3721 atcaataatt tttcctcttg ccattgtatc atccaactca attcccattt gtgctgctgc
     3781 tttgcgcata gccatttcat ccatttcact gacatctaca cctgtaaaat gctcaatagc
     3841 ttcatacatg gtaaatcgtt tccatgggcg tttaaaatca attgtcttct ctcctacttt
     3901 cacagcagtt gagccatgca attctaaagc tactttctca atcatttcct ccaccaggtt
     3961 catcatccac tcgtaatctt tataagctac ataaagttct atctgtgtaa actccggatt
     4021 atgaaacctg ctcatacctt cgtttctgaa atctttcgaa aactcataca caccatcata
     4081 gccaccaaca attaatcttt tcagataaag ttcgttggca atgcgcaaat acaatgtcat
     4141 gtccaatgta ttgtgatgcg ttttaaatgg acgcgctgca gcaccaccat ataaaggttg
     4201 taaaattggt gtttctactt cgagataacc tttttcgttc aggtaattgc gcatggtatt
     4261 caccaatcgt gtacgtttac ggaatgtttc ttttacctgc ggatttacaa ttaaatcaac
     4321 ataacgctga cggtatctta aatcaggatc tataacggca tcaaaaactt ctccgtcttt
     4381 ttcttttaca ataggtaatg gacgaagtga cttacacaac agtataaagg aacttgcatg
     4441 tatagaagtt tctcctgttt tagttttaaa aacgtagcct ttaatgccaa taatatcacc
     4501 tatatcaata agttttttcc aaacagtatc atacaaagtt ttatcatcac ctttacaaat
     4561 ttcatcacgt tttatgtata tctgaatttt tccttctgca tcctgtatct gtgcaaacgc
     4621 agccttaccc atatctctta cactcatgat gcggccacca atactcacct cttcttttcc
     4681 tgcaccaaaa cgtgcttctg cctgttcgta gttttcaaga ataaaggtgc tgtttgtgtt
     4741 tatttcaaac ttctctgccg gatagggatt aattcccatc tgtaagagct cattcagctt
     4801 ttctcgtcta atttgttctt gctctgataa cgccattgtt tttaaatttg attgcaaaga
     4861 tacatgttgc cgcttgcaat tatccctttc ggtctgacag tagtttttcc tgcttaggtg
     4921 ctcacaattt cccgatgagg acaaactatt tgtcggcaca gcaatgcata tctatttttg
     4981 actcattgat taaagattat tctctgttaa aatattttta gttcagggtg ggtttaactt
     5041 cccccgtcag caaaactgac ggggtttttt tatgtctcca taaaaaaagc cacccttttg
     5101 gagtggcctt tatattgttt tggtttaatt aaccaatctg aacacgtctg aaagctttta
     5161 cggtaagccc tttttccact ccatccaaat actgacggat ggttttttta tcttctttta
     5221 taaaagcttg attcaacaat gttgattcct gataaaattt attcagttta cccattgcta
     5281 tcttctcaat catattctcc ggtttgcctt ctgcacgtgc ctgctcttta ccgatttcta
     5341 tttcgcgatc taatgtactt tgatccacat cgtctttatc aattgcaaca ggattcattg
     5401 cggcaatttg catagccaca tctttaccgg cttcattagc tgcagtagca gatgataaac
     5461 ctaccataga aactactctg tttccggggt gaatataaac cactacttta ggagcttcga
     5521 ttacttcata acgagtaatt tcaatcttct caccaatttt tccaaccatt tcaatcagct
     5581 tctcactgat tgttgtgcct tgtaattgta aagccagcaa tgcatcaaga cttgctgcat
     5641 tatttgtcaa tgctgtttca agaacatctt cagcaaattt tacaaactct gcatttttag
     5701 ctacaaagtc tgtttcgcaa cttaatgcaa ctaaatatcc tttagtagca ttgttatttg
     5761 ttttagcaac aatgtatcct tcttttgctt cgcggtctgc tctgttagct gctactttct
     5821 gtcctttttt acgaagttca tcaattgctt tttcaaaatc gccattggct tccatcagtg
     5881 cttttttgca atccatcatt cctgcaccgg tcatttgacg cagtttgttt acatcgcttg
     5941 cactaattgt tactgtactc atttatatta ttaatattta aatcttaaac taaattaatc
     6001 tgctttatct tttgctggtt gtggtacctt tacgtggacg tgtggtggct ttttttgcag
     6061 gagattttgt ttttgtttct ccactatcat caccttcgtc agacatggta aattcttttt
     6121 cgccttcgcc tctttcgcca ccttcttctt ctgcagcagc ttcgcgttcg cggtcagctt
     6181 ctttgtccat tttacgttct tcaagacctt cttcaatagc cttaatcatg gcattcataa
     6241 ttaaagaaat tgatttagtt gcatcatcat ttgccggaac agggaagtca attaacgtag
     6301 ggtctgtatt ggtgtcgcag atagcaatag tagggattcc taatttctgt gcttctgaaa
     6361 ctgcaatatg ctcttttaaa acgtcaacaa taaaaagtgc agcaggcaaa cgggtaaggt
     6421 cggcaatact accgataagt ttttccattt tcgctttctg acgcgatacc tgaagacgct
     6481 ctttctttga tatgttgttg aaagattcgt ctttcatgaa acgatcaaag cctgtcattt
     6541 tcttaactga cttgcgctgt gttgcaaagt tggtaagcat accaccgggc caacgctcag
     6601 tgatatatgg catgccaaca gatttggctg cattggcaac aatttctttt gcttgtttct
     6661 tagtagcaac aaacaatatt tttttgcctg actttgcaat ttgttttatg gcagcagtag
     6721 cttcttcaag tttagcagct gtcttattca aatcaataat gtgaatgcca ttgcgctcca
     6781 taaaaatata aggagccatt ttaggattcc acttacgttt caggtgtccg aaatgacatc
     6841 ctgcatctaa caattcctgt gtattaatag acattttttg ctggtataat tttttaacgt
     6901 ttgctgaact ggaatttctt acgggctttc ttctgaccgc tcttcttacg ctcaaccatt
     6961 cttgggttac gtgtcagtag tccttctttt ttcaaagccg gacggttttc aggattcatt
     7021 tcgcacaatg cacgcgaaat agctaaacgg atagcttctg cctgtccggt aattcctcca
     7081 cccattacat tggccttaac atcaaactgc tcgtttgcat tgataactgc aaatgcctgt
     7141 gttactttat tctgaagcgg accggtagtg aaatagtttt tgaattcacg attgttgatt
     7201 acaatagttc ctgttccggg agtcagataa acacgcgcta cggctgtttt gcgtcttcct
     7261 attttatgta tattattcat tgccgattaa atatctaaag ttattttacg gggattttgt
     7321 gcttcgtgac tatgttcact gccagcaaat acccttaggt ttttctttaa agcatttccc
     7381 aatctgtttt taggaagcat tttatgaatg gcattttcca gaattctttc aggataggta
     7441 tccatcagtt tttcaggtga tgtgaaacga tgacctccgg gatagcccgt atggcgcaca
     7501 taaaccttct gtgccatttt tttaccggta agttttacct t
//
LOCUS       DAORQT010000064         7466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1487_length_7466_cov_9.931048, whole genome shotgun sequence.
ACCESSION   DAORQT010000064 DAORQT010000000
VERSION     DAORQT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_7466_cov_9.931048"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="PK134_03645"
     CDS             complement(<1..93)
                     /locus_tag="PK134_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRB37973.1"
                     /translation="MNKKILIFAALSGLCAVALGAFGAHKLKELL"
     gene            complement(93..629)
                     /locus_tag="PK134_03650"
     CDS             complement(93..629)
                     /locus_tag="PK134_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37974.1"
                     /translation="MEELGIGSRIRHPEFGIGVVINVKPRTYITVFTERGKIEVAKTY
                     QNLEVIDAQPPVGDPVSFADMERVFTNIIKKYSDVQETVPLHGRWIGGKVVLHPNNPS
                     QKPKEIPMDVFFNKIIMIRDRLRVMEQRINAHDKLTEEDKINLQQYLTRIYGSLTTFN
                     VLFKDEEDVFVGEKGNKE"
     gene            complement(649..981)
                     /locus_tag="PK134_03655"
     CDS             complement(649..981)
                     /locus_tag="PK134_03655"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HRB37975.1"
                     /translation="MRIIGFIFSILFIVSCTNKPAEVKTPAPLINNVTVQTYANDSTL
                     GGYGYDILVDGKIMVHQPNIPAVMGNRGFATEEDASKTATLVSFKLKNNIMPPSVTLE
                     ELDSIGIK"
     gene            complement(1015..2631)
                     /locus_tag="PK134_03660"
     CDS             complement(1015..2631)
                     /locus_tag="PK134_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB37976.1"
                     /translation="MLFILLSTVNAEGQMWQWAKSDGSLNNDAAISVSTDYAGNVFVA
                     GIFRDSIFSGANKLYSYGLDDIYVVKYSPNGNILWTKCFGGTGNDGYGLYIKADSIGN
                     CYVTGAYSYSAQFDLITLNSGFVNNMFLVRMNPTGYILFANDFQGTNGSVGYALEVDR
                     NQNCYLTGYFYDQLFAAPNTVYSNGSHDIFLAKIDGLGNVLWSRRAGGSSYDSGGGVA
                     LDDFGNIYITGYFSSTADFDIATLISSGGDDAFVAKYDNSGNFKWVSRTFGSGVYDIG
                     VKIRIQQDKICVVGQFEDTMTIGLQTLISYGGMDGFITLLDSSGNFLWAKQLGSVGND
                     RFSDVAFVSNSEIVASAWINQTAFLNNTLINVFNKSGLVSWFDYNGNIISYKQASNIA
                     KNSFNALCTNISNEILVAGSMEDTLQLDSIKLASVGLADMIIGKIVKRETAVTEVQDN
                     EFLVQNNLFSDETRPSIIFSEQVQIKSVEIFDMNGNCVVKYFPQRKLDVGNKFTINNP
                     VMSQLSQGVYLVRITTGISDKIVLIKIIKV"
     gene            complement(2655..3821)
                     /locus_tag="PK134_03665"
     CDS             complement(2655..3821)
                     /locus_tag="PK134_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRB37977.1"
                     /translation="MKKILIAILLSSFNGYGQQLVWTQKADIPSSTRHSPIGFSINQY
                     GYIATGFNNGTCYNDMWKYDPSTNVWSQVASLPGSGRYGASVFVINSKAYAGIGWSSS
                     NQGLSDFYEYDPIANQWSARANFIGPQIYTAIGFAVGGLGYITSGYPLNSSVFEYNPI
                     NNVWFSKSGFPGNPRQSATSFVVNDTAYMVSGYLTSSPSNELWRYNQLSDTWTMMQSL
                     PNNPRYGAVGFELSGLGVITTGGNGTYYFTDGYAYSPPINQWLNIPQFTGAIRRHSAS
                     FTINNKGYVSCGISATGSTLNDLWELSDVTALPEAASTQPKVYYNQSYSTLTVTGLAN
                     KKSTLKIFSLSGQMVYSQQCYSDMEQIPLKLNKGIYSYNIEGIGKMMNGKFEKL"
     gene            complement(3875..4597)
                     /locus_tag="PK134_03670"
     CDS             complement(3875..4597)
                     /locus_tag="PK134_03670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB37978.1"
                     /translation="MTKSYKEVAEYYDELWADLEKQSMAGVNSRHRVILNKLKKAGFN
                     KNSTLLEIGCGVGTLSNFLATQVSSGSIDAVDISPASIEYAKKKYSEVKNLSFHVSDM
                     TDFKINKKFDFILFPDVLEHIPVEAHDNIFKTISGLVHANSQVAINLPDPRSIRWLAK
                     NKPELLQIIDQDIEANVLIDKMYSNGFFLFTMETYCLYFTMPDYGWFVFKVKKEYETT
                     EKIAQSTVLLNGIKLRLRNLFS"
     gene            complement(4686..5738)
                     /locus_tag="PK134_03675"
     CDS             complement(4686..5738)
                     /locus_tag="PK134_03675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB37979.1"
                     /translation="MKKKKLIFVFPNYSTFVQRDIELLQERYDVITFSFNPRNKIFIP
                     LAFIRQFFFLLFYLPSASVVVGQLAAYHTFLPVLMAKLFRKPSVIFLAGTDSAFFPSI
                     QYGNFCRPLLRWFTIASIRMATHLAPKHQSLIHFNYDYDASGAPVQGVKHFIKNFKTP
                     YTAIANGFDSNKFFPLQTERRKNTFITVAVGMGTGNINQLKGVDLILQIAPLFPDCAF
                     TLVGMSTTTLDTLPPNVKALPLQNEKQLQQLYSSHQFYMQLSLSEGFPNAVCEAMLCG
                     CIPVVSNVNALPEIAAEGFVLQHKNKDMLVALIQKALTSDSEKLSKAARLHIIKDYSL
                     EIRKERLFALLNLLKA"
     gene            complement(5875..6280)
                     /gene="tnpA"
                     /locus_tag="PK134_03680"
                     /pseudo
     CDS             complement(5875..6280)
                     /gene="tnpA"
                     /locus_tag="PK134_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6322..7269)
                     /locus_tag="PK134_03685"
     CDS             complement(6322..7269)
                     /locus_tag="PK134_03685"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB37980.1"
                     /translation="MIDFVAPLTKNKLVLSDSKLVSDSGEKFPVIDGIPRFVELDNYA
                     NAFGLQWKSFAKIQLDSYNGSTISKDRLVRCLGTSLAELQGKTILEVGCGAGRFTEHL
                     VSSGALVHSVDLSAAVEANHDNISDKPNYRIAQADVYKLPFNENSFDIVICLGVIQHT
                     PSPEKTIHALWNMVKPGGTLVIDHYTFDWMYLLKPIMFYRFFLSRMKPEKSKRIVESL
                     VGFFFPLHWKYRSSKLMTMLLNRLSPCYVYIKTFPKMDYDFHFHLTRLDTYDGLTDYY
                     KHLRSKTQIENILKKFKPHSLSVWKGGNGVEARAIKPIG"
BASE COUNT         2446 a         1478 c         1159 g         2383 t
ORIGIN      
        1 caacaactct ttaagcttat gtgcaccaaa ggcacccaat gccactgcgc aaaggcctga
       61 tagggcggca aaaatcaaaa tcttcttatt cattattcct tatttccttt ctcgcctaca
      121 aaaacatctt cctcatcttt aaacaaaaca ttaaaggtgg tgaggctgcc ataaatacgt
      181 gtcagatatt gctgaaggtt gattttatct tcttcggtta atttatcatg tgcgttgatg
      241 cgctgttcca taacgcgcag tcggtcgcga atcataatta ttttattaaa gaaaacatcc
      301 ataggaattt ctttaggctt ttgtgaagga ttgttgggat gcaacacaac cttacctcca
      361 atccagcgac catgcaaagg cacggtctcc tgcacgtctg aatatttttt aatgatgttt
      421 gtaaacacac gttccatgtc agcaaaactc acagggtcgc ccacgggtgg ttgcgcatct
      481 attacttcca ggttttgata tgttttagca acttctattt taccacgctc ggtaaatacc
      541 gtaatatagg ttcgtggttt tacattgatg accacaccaa tgccaaattc gggatgtctg
      601 atgcgtgaac ctattcctaa ttcttccata taaaatattt agtaagtatt atttaatgcc
      661 tatactgtct aactcttcaa gagtaacaga gggcggcatg atattgtttt ttagtttaaa
      721 agatacaagt gttgctgttt ttgaggcatc ctcttctgtg gcaaagccac gattacccat
      781 tacagcaggg atgttaggtt gatgtaccat aatttttcca tcaactaaaa tatcgtagcc
      841 atagccgcct aatgtgctat cgtttgcata agtctgaaca gtgacattgt tgattaaggg
      901 ggctggagtc tttacttcag ccggcttatt agtgcaagat acaatgaata gtatcgaaaa
      961 gataaaacct attattctca tgtctcaaaa ataaatggtt ttatataaaa gaactcaaac
     1021 tttaataatt ttgattaaca caattttgtc ggaaataccg gttgtaattc tgactaaata
     1081 aacaccttgt gaaagctgtg acattacagg attgttaatt gtgaatttat tccctacatc
     1141 aagttttctt tgaggaaaat attttaccac acaattgccg ttcatatcaa atatctctac
     1201 agatttaatc tgaacttgct ccgaaaaaat aattgatggt cgagtttcat ctgaaaataa
     1261 attattctga actaaaaatt cattgtcctg tacttcagta actgctgttt ctcttttcac
     1321 tattttacca attatcatat cagctaatcc aacagaagct aatttaatac tatccaattg
     1381 cagtgtatct tccatagaac ctgcaactaa aatttcattg ctaatattag tacataaagc
     1441 attgaatgaa ttttttgcaa tattggatgc ttgtttataa gaaattatgt taccattata
     1501 atcaaaccaa cttaccaacc cacttttatt gaatacattg attaaagtat tatttaaaaa
     1561 tgcagtttga ttaatccagg ccgatgcaac tatttcacta ttggaaacaa aggcaacatc
     1621 agaaaaacga tcatttccta cactacctaa ttgcttagcc cataaaaaat taccggaact
     1681 atccagtagt gttataaatc catccatgcc tccataacta atcaatgttt gtagtccaat
     1741 tgtcattgtg tcttcaaact gtccaacaac gcaaatttta tcttgttgga ttctaatctt
     1801 tacacctata tcataaactc cacttccaaa agttctgcta acccatttga aattaccgct
     1861 attgtcatat tttgctacga aagcatcatc gcctccacta ctaattaatg ttgcaatatc
     1921 aaaatctgca gttgatgaga aatatccggt aatataaata tttccgaaat cgtctaatgc
     1981 aacacctcct cctgaatcgt agctgctacc tcctgcccgc ctagaccaaa gaacatttcc
     2041 taagccatca attttggcaa gaaaaatatc atgactacca tttgaatata ctgtattcgg
     2101 ggctgcaaat aattgatcat aaaaatagcc tgttaggtaa cagttctgat tcctgtcaac
     2161 ctccaatgca taaccaacac tgccatttgt tccttgaaaa tcatttgcaa acaaaatata
     2221 tccggtaggg ttcatcctta ctaaaaacat gttatttaca aaccccgaat tcaatgtgat
     2281 taaatcaaat tgtgctgaat aactgtaagc acccgttaca taacaatttc caattgaatc
     2341 agccttaata tatagaccat aaccatcatt tccagttcca ccaaaacatt tagtccagag
     2401 aatgtttcca ttcggactat acttaactac ataaatatca tcaagaccat aactatacaa
     2461 tttatttgct ccactaaaaa ttgaatctct gaaaattccg gcaacaaaaa cattacctgc
     2521 atagtctgtt gagacagaaa tagcagcatc attatttagc gatccatctg atttagccca
     2581 ttgccacatt tgcccctctg cattaactgt actcagtaaa ataaataaca ttgcggaaat
     2641 tatattctta attctcataa cttttcaaat ttcccattca tcattttacc aattccttca
     2701 atgttgtaag aataaattcc tttattgagt tttagcggta tttgctccat atctgaatag
     2761 cattgttgcg aataaaccat ctgccccgac agagaaaata tttttaatgt tgatttctta
     2821 ttcgccaatc ccgtaaccgt tagtgttgag tatgattgat tataataaac tttaggttga
     2881 gtgcttgccg cttcgggcaa tgccgttaca tcagataatt cccagaggtc gttcaaagta
     2941 cttccagttg cagaaatccc acatgaaaca tatcctttat tattaatagt aaatgaagca
     3001 ctatgtcttc ttattgctcc agtaaactgc ggaatattta accattgatt tattggaggc
     3061 gagtatgcat atccatcggt aaaataataa gtaccgtttc ctccagttgt gataacacct
     3121 aaacctgaca actcaaaacc aacagcaccg tatcttggat tatttggtaa tgactgcatc
     3181 attgtccaag tatctgaaag ttgattatat cgccataatt catttgaagg acttgaagtc
     3241 agataaccag acaccatata agcagtatca ttgactacaa aggaagttgc actttgacgt
     3301 gggttacccg gaaacccgga ttttgaaaac caaacattat ttattggatt atattcaaat
     3361 acagaactat tcagaggata tccagaggta atataaccta agcctccaac agcaaaacca
     3421 atagctgtat aaatttgtgg tccaataaaa tttgcgcgcg ctgaccactg attagcaatg
     3481 ggatcatatt cataaaaatc actcaacccc tgattagaag aactccatcc tattcctgca
     3541 taagctttac tattaatgac aaatacacta gctccatatc gtccacttcc aggaagtgaa
     3601 gcaacctgcg accatacatt tgtgcttgga tcatatttcc acatatcgtt atagcaagtt
     3661 ccattattaa aaccagttgc tatataacca tactgattaa ttgaaaatcc tattggtgaa
     3721 tgtctagtac tacttggaat atctgctttc tgagtccaga ctaactgttg gccataacca
     3781 ttaaatgaag ataataaaat tgcaatgaga atctttttca tataataatt gcttcaaagc
     3841 aaatttataa ttaaagacta ttaaaaacaa taaatcaaga aaataaattc cgcaaacgaa
     3901 gttttatccc attaagtaat acggtagatt gtgctatttt ttcagttgtt tcatattcct
     3961 tcttaacttt aaaaacaaac cacccataat caggcattgt gaagtacaag caatatgttt
     4021 ccattgtaaa aaggaaaaat ccatttgaat acattttatc aatcaacaca ttagcctcaa
     4081 tatcctgatc tataatttgt aataattcag gtttgttttt tgccaaccat ctaatacttc
     4141 ttgggtcagg aagattaatg gctacttgag aattagcatg aactaaacca ctaattgttt
     4201 tgaaaatatt atcatgtgct tctacaggaa tatgctctaa tacatcaggg aaaagaataa
     4261 aatcaaattt tttattgatt ttaaaatcag tcatatcaga aacatgaaat gaaagattct
     4321 tcacttcact gtatttcttt tttgcatatt caattgatgc cgggctaata tcaactgcat
     4381 caatactacc tgaacttact tgagtagcga gaaaattaga gagtgttcca acaccacaac
     4441 caatttctaa taaggttgaa tttttattga atccggcttt cttcaacttg ttcaaaatga
     4501 ctctgtgtct gctattgaca ccagccattg actgcttttc aaggtctgcc cataattcgt
     4561 cataatactc agcaacttcc ttataactct tagtcattaa aattcaattt aaaaataaat
     4621 tatgcctgcg aaaatagtct aaaaattcaa acctttccct ttgtgctaaa ataaagcagt
     4681 taggtctatg ctttaagtaa attcagcaat gcaaacagcc tttctttcct tatttcaaga
     4741 gaatagtctt tgatgatatg tagccttgct gctttcgata gtttttctga atcagaagtc
     4801 agtgcttttt gaatgagtgc aaccagcatg tctttatttt tatgctgtag aacaaagcct
     4861 tcagcagcaa tttcgggcag tgcattaaca ttagaaacta caggaataca accacacagc
     4921 atggcttcgc aaacagcatt aggaaaacct tcgcttaacg aaagctgcat ataaaactga
     4981 tgcgatgaat aaagctgctg cagttgtttc tcgttttgca agggcaatgc ttttacattc
     5041 ggtggcaatg tatccaaagt cgttgtcgac atgccaacga gtgtaaatgc acagtcaggg
     5101 aaaagtggtg ctatctgcaa aataaggtca acacctttta gctgattaat gttgcccgta
     5161 cccataccta cggcaacggt aatgaatgtg ttttttctgc gttctgtctg taaagggaaa
     5221 aatttattac tgtcaaaacc attggcaata gcagtgtatg gcgttttaaa attttttata
     5281 aaatgtttta cgccctgcac aggagcaccg gaggcatcat agtcgtaatt gaaatgaatg
     5341 agcgactgat gtttgggggc taaatgcgtg gccatacgaa tacttgcaat agtaaaccat
     5401 cgcaacagcg gcctgcaaaa atttccatac tgtattgaag gaaaaaaggc actgtctgtc
     5461 cccgctaaaa aaatgactga tggcttgcgg aaaagctttg ccatcaacac aggtaaaaaa
     5521 gtgtggtagg cagccagttg accaacaact acagatgcag aaggcagata aaataacaga
     5581 aaaaaaaact gcctgatgaa tgcaagagga ataaaaattt tatttcgtgg attaaaagaa
     5641 aaagtaatca catcgtaacg ctcttgcagc aattcaatat ccctttgtac aaaggttgaa
     5701 tagttaggaa aaacgaaaat tagttttttc tttttcaagg aaaatatttg attgcgaaag
     5761 taaggatttt ggaggtgggg atcttcctca catcaggact ttagtcctgt aaccccatgc
     5821 tgaagcatgg ggttactcta tacgatgcct ggtacctcac actttagtgt gaggtcaaag
     5881 aaactcatca cattcctgag taaaaggaat tttcttgtga tgctcctcct gattgtcaat
     5941 atactttctt actgcatcaa gagcagattc gctaactgat gctgcataat attcattagc
     6001 ccattcgaat tttgattctg taagttgttc tttatttgcc caaaaagccg attcgccctt
     6061 tatcaactgc atcgctttag caatactcat atcagcattc aaccacatca aacagtgcaa
     6121 atgatctgta tggccattaa ttctgtcaat aaaaatattt ttcaccttgg cattttctct
     6181 gatatgtttt attagctttt tacgtgcttc tttagtgaga aaaggatgtc tgttttttgg
     6241 taccccaaac aacatgcaca ataattttca caaatgccat tctatatgtg gtttgatatg
     6301 aagataaaac tttgtggaat atcagcctat aggcttaatt gctcttgcct caacaccatt
     6361 cccacccttc caaacactta aggaatgtgg cttaaacttc tttaaaatat tttcaatttg
     6421 tgtcttactt cgcagatgct tataataatc tgtaagtcca tcatacgtgt caagtcttgt
     6481 caggtgaaag tgaaaatcgt agtccatctt cggaaatgtt ttaatataaa cataacatgg
     6541 cgacaaacga ttaagcaaca tagtcatcaa cttactactt ctgtatttcc agtgtagcgg
     6601 aaagaaaaat ccaacaagac tttcaactat tctttttgat ttttcaggtt tcattcttga
     6661 tagaaagaaa cgataaaaca tgataggttt caataaatac atccaatcaa acgtatagtg
     6721 atcaataacc aatgtaccac caggtttcac catgttccac aatgcatgaa ttgttttttc
     6781 gggagatggt gtatgttgaa tcacaccaag gcaaataaca atatcaaagc tattttcatt
     6841 aaaaggcagc ttgtaaacat cagcttgcgc aattctataa ttgggtttat cactaatgtt
     6901 atcatgattt gcttctactg ctgcactcaa atcaacagaa tgaaccaagg caccactact
     6961 caccaaatgt tctgtaaaac gtcctgcgcc acaaccaact tcaagtatag ttttaccttg
     7021 caactctgct aatgatgtac ctaaacatcg tactaaacga tctttagaaa tagtgctgcc
     7081 attataactg tcaagttgaa ttttagcaaa agatttccat tgtaacccaa aagcgttagc
     7141 ataattatca agctcaacaa aacgcggaat tccatctata acaggaaatt tctctccact
     7201 atctgatact aatttactat cagacaaaac aagtttgttt tttgttagtg gtgcaacaaa
     7261 atcaatcatg cgcgcgaaag tattgaaaag attgtttagc tatcttttct aacaacaatt
     7321 ttacatgttg ctttctgatt cctgttatca aaacaaaaac cataataaac acctgataat
     7381 aatttcctat caatatttat tctgtttttt ccaacttgaa caactttact caataccacc
     7441 ttacctcttg catcaatcaa agttag
//
LOCUS       DAORQT010000065         7447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1497_length_7447_cov_7.538285, whole genome shotgun sequence.
ACCESSION   DAORQT010000065 DAORQT010000000
VERSION     DAORQT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_7447_cov_7.538285"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2600
                     /locus_tag="PK134_03690"
     CDS             <1..2600
                     /locus_tag="PK134_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37981.1"
                     /translation="ATPNIISQNKKITLSQQNELDFNLISDNELIIENIFGFEVDIQS
                     KVKQSDGIWLVSGNLINLPANDNFSLHDSKQKISFTDLKIKKSDATKNGIPVGVSVEN
                     TFSTDLTNIKLLLQNSFGVIQKPSSGDVLQVKSDNQKGRLTGKIAVQKSSFKFTQSYI
                     TFNNDPNEAMLITDKPGSFITDISSIDIATSTKKKFGLANLQGKTMKFSLLGFDAEAD
                     AINSWLQDNSINLQTIIHINALPDMTPSTLDINAGNLIIHPEKLEPLKGNQPIKFNLE
                     KWQFIGNNWQLSQGDNSINIATGTIKTGSIDIPISDISLQPDHIGIGDFDVNNLSFGG
                     IIPVHVTSTNPVFGRNKSIGSDQKPHYEFRLIGENGSPGVVIKTLPGMKTGEEMKFQN
                     LSLISNGEQIINPGNQPNSITFYSVMKVKPLSFMSGQDYVNMTCGIDLGIPQLKETSG
                     IIQFSKEAGQIKFQLYPLNVSLKGPGDVDFVANVQFNDRPQNLTEGKFTALGTIYEKE
                     GMVLKAILNKTTTAAWIQVDPENQKLPLGGSTTSLANIKGKMEADMSSGIWKNFTFSG
                     EMQGFKGMQGDTRKTFVVTGSINASNQNIEVKNIPSGFGNIGLNYDFVNSRFTGNLQL
                     DKNIGPLALAGTANLLVDPDGWYFLAGGKVQAPGIGAMSAGLLIGDYSKMPGDVSGAL
                     MQFAYDKHVPPSFQNGISGFFFTGMKELPVLNIPNYSIDLGVISASFGAQAGLDARLW
                     MDFASAGNEYGIGAMVFAHAYLKGASITCTKFGADARAELGMKGSYATSTGTFSLNGC
                     GSFTISGSIKQCFPTPCFSDGICCEGCGGIGVSQGIKVNLLLDSNGATDLSFGFGNCS
                     GQQVMGGSW"
     gene            2662..4644
                     /locus_tag="PK134_03695"
     CDS             2662..4644
                     /locus_tag="PK134_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37982.1"
                     /translation="MKRFLLSIIIFCTIINITVAQEQAGSIPVAVPVAQGVWVYLGNS
                     IPRNMHYQIERSKGDSKRFEKLGETTAPSSEQEMKNRQLPFSKYFEKLDPINDKTINR
                     LWQSTQKYPKADSLFSSNLPMMHLMAGTAYFDATAEKNENYIYRISLIASDGKSIFTK
                     ESNATAQFKKASLPKINFESCKYAEGKLVLTWSVKDPLRMSHFNVYRTIFGKEEYAKI
                     SIERGVYSENNNLMLLAIDTIGNKPAWYEYKIAAVDAYGNEGELQAIASGGSVEDYYA
                     PPITNLKAINTNSNHEVRLSWRLEKKKYLNGINVMRSTLFDSGYQRIATLPVSDTSFT
                     DIVPLSGENYYYYLQILSANDKPVSSAKVFAAYSGDKSVPLPPKEIDATSQPKGINVY
                     WKSDEPFAKGFYVYRRDNTTVPFVLISPLIASRQPIYSFSDTSQQLQAGGVYQYIVRT
                     ISDNNQLSKPSDTVSANPGIKKAMTPPTNLRFRENNGMLTLLWDDMRNWESNLMGYKV
                     YRKTSNGAFVKMDNDSLKSERNFFEDSELSSGTSYVYGVKTFDNSGSESEMATISFTA
                     VGELLAGPPSGISVSQTGDNVFITWGQISGDIVAIKIYKSEKGIQPKLIATITDVDSY
                     TDKNVFKGKLYFYQLSTVGKSNKEGAQSEKISIRVK"
     gene            complement(4657..5286)
                     /locus_tag="PK134_03700"
     CDS             complement(4657..5286)
                     /locus_tag="PK134_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37983.1"
                     /translation="MLKTGNTHFNIGILNVDSLFADAFLRLLNSFNDNLTIKHVSEAS
                     IASFYSFDILFVHQSRTWDYLNFLLSQKSNINAHETIYCLNCNTSLLLQKDVTDTLKI
                     LDLTIPVNDIRENLQHHVRKINKGKAKFGRPARSLTVLSRNFQLAGHFSDEELNIIYS
                     LRKHICNKQISVSCKISVSTLKRRLKAIADKCGVPNNKAAILCHLYERM"
     gene            5545..7443
                     /locus_tag="PK134_03705"
     CDS             5545..7443
                     /locus_tag="PK134_03705"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRB37984.1"
                     /translation="MKKIKLIIILLLVSNMLQSQTIVLQETFDGQVMPTGWHEDSAGF
                     APVINQWNFIYYPFTMNGIGFDASFIRTGIANHDYSLVSPSFSTVGLSDVYLSYSDYH
                     QGPIDSGYQNVEMSVDSGQSWTIMLSNNGYNHYLGQNGSRRVVHLGTNAANQPNVKIR
                     FHFKVGGGWRWAIDSVVVNDYQLCTTPTDSTGVVYSSKDFLCSGIAGFFWIDHVSGGI
                     GQTYQWQYKPWGSSSYLNIAGAVYDTMTFVQNTPNYYRCLVVCNADTAGSMVTFVTDS
                     PTVVPKLRATNFSGICPGSPDTTYIFCEKNIPGIGYQWQWKQNIADPFTDIAGANDTL
                     YAIDAAVFPNQIYLRCKQQCIQSGKYLATATEVIFENPNPSCYCIPYNQNYCGPSTPI
                     FDGGIVDMHIAGTTLNNPSSTCSNIYGYPGQLSNYTYFDPTVANQTATLSRNVPYSMT
                     ITTDTLQIWFKGGLWIDYDHDSYFENTEYTTIDSLYPGTPRTITFSIPATATPGLTGM
                     RLRVAGNFPSVLNANSACTYAYGGETEDYMITIDTIVGIGEVKQHEANVQLYPVPASG
                     YIVVQSSQTIRSVRLESTTGALVKQDNGLNTNNWRMDVSSYAPGMYLMRTETSAGVVM
                     KKVMISRE"
BASE COUNT         2533 a         1274 c         1454 g         2186 t
ORIGIN      
        1 gtgctacgcc caacatcatc agccagaata aaaaaattac attaagtcag cagaatgaat
       61 tggattttaa tttgattagt gataatgaac tcattattga aaatattttt ggatttgaag
      121 tggacattca atccaaagtg aaacaatcgg acggcatttg gttggtgagt ggtaatttga
      181 taaatctgcc tgctaatgat aatttttcat tgcacgacag caaacagaaa atatctttca
      241 ccgatttgaa aataaaaaag agtgatgcta caaagaatgg aattcctgtt ggtgtttctg
      301 tggaaaacac cttttcaacc gatctcacaa atattaaatt gcttttgcaa aattcctttg
      361 gagtaattca aaagccctct tcaggtgatg tgttacaggt aaaatccgat aatcagaaag
      421 gtagattaac aggaaaaata gctgtacaaa aatcgtcatt taaatttaca cagagttata
      481 tcactttcaa taatgatcct aatgaagcta tgttgattac tgataaaccg ggttcgttta
      541 taactgatat ttcttcgatt gatattgcaa cttccactaa aaagaaattc ggattggcta
      601 atctgcaagg aaaaacaatg aagttttcct tacttggttt tgatgcagag gcagatgcta
      661 ttaattcctg gctacaggac aacagtatta atttacaaac catcattcat atcaatgcac
      721 tgccggacat gactccttct acactcgata taaatgcagg aaacctcata attcatcctg
      781 agaagttgga gccacttaaa ggcaatcaac ctataaaatt caatttagaa aagtggcaat
      841 tcataggtaa taactggcaa ctgtcacaag gcgataattc aattaacatt gctacgggca
      901 caattaaaac cggaagtata gatattccca tatctgatat ttctttacag ccggatcata
      961 ttggcattgg agattttgat gtaaacaatc tttcattcgg tggtatcatt cctgttcatg
     1021 taacatcaac caatcctgta ttcggacgaa acaaaagtat tggctcagat cagaaaccac
     1081 attatgaatt tcgtttgata ggtgagaatg gaagccccgg tgtagtcatt aaaacattgc
     1141 ccggaatgaa gaccggtgaa gaaatgaaat ttcaaaatct ttcattaatc agcaatggag
     1201 aacaaattat aaatccgggt aatcaaccaa actcaattac attttatagt gtgatgaaag
     1261 taaaaccact ttcatttatg agtggacagg attatgtaaa tatgacttgt ggtatagacc
     1321 ttggaattcc acagttaaaa gaaaccagcg gcatcattca attttcgaaa gaagcaggac
     1381 aaattaaatt tcagttgtat ccattaaatg tatcactaaa aggaccggga gatgttgact
     1441 tcgttgcaaa tgttcagttt aatgatcgtc cgcaaaatct tactgaagga aaattcactg
     1501 cacttggtac tatttatgaa aaagaaggaa tggttttaaa agcaatttta aacaaaacta
     1561 ctacagcagc atggatacaa gttgatcctg aaaatcaaaa attaccatta ggtggaagca
     1621 ctactagtct tgccaacatt aaaggaaaaa tggaagccga tatgagttcc ggaatatgga
     1681 aaaattttac tttcagtggc gaaatgcaag gcttcaaagg aatgcaaggt gatacaagaa
     1741 aaacatttgt agtgacgggt tccatcaatg caagtaatca aaatattgaa gtaaaaaata
     1801 ttccttccgg atttggaaat attggactta actatgattt tgtaaacagc cgattcacag
     1861 gtaatttaca gttagataaa aatataggac cattagcttt agcaggtact gctaacttat
     1921 tggttgatcc tgatggatgg tattttcttg caggaggaaa agttcaagca cccggaattg
     1981 gtgccatgtc tgccggattg cttattggag attatagtaa aatgcccggt gatgtttccg
     2041 gtgcactcat gcaatttgca tacgataaac atgtgccacc aagttttcaa aacggcattt
     2101 caggattttt ctttacaggt atgaaagaat tgccggtgct aaacattcca aactatagta
     2161 tagaccttgg tgttatcagt gcttcgtttg gcgcacaagc cggattagac gcacgattat
     2221 ggatggactt tgcttcggca ggaaatgaat atggaattgg tgcaatggtg tttgcacatg
     2281 catatttaaa aggtgcttca attacatgta ccaagtttgg cgcagatgcc agagcagaat
     2341 taggaatgaa aggaagttat gcaacatcta ccggtacatt ttctttgaat ggttgtggga
     2401 gttttaccat aagcggaagt ataaaacaat gctttcctac tccttgcttt agtgatggaa
     2461 tttgttgcga aggttgtggt ggaatcggtg taagtcaggg tataaaagtt aatttattgt
     2521 tagactccaa tggtgctact gacttgagct ttgggtttgg gaattgcagt ggacaacagg
     2581 tgatgggtgg cagctggtaa atccaatctt gtactttaga atagattatt taaatggtga
     2641 ataaaacaaa atctttaaaa aatgaaacgg tttcttcttt caattatcat attttgtact
     2701 attattaata ttactgttgc acaagagcaa gccgggtcaa tacctgttgc tgttcctgta
     2761 gcacaagggg tgtgggttta tttaggaaat agtatcccac gaaacatgca ctatcagatt
     2821 gagcgtagca aaggcgatag taaaagattt gaaaaacttg gtgaaacaac agcaccttca
     2881 tcagaacagg aaatgaaaaa ccgtcaactc ccctttagta aatactttga aaaattagat
     2941 cctataaatg ataaaacaat caatcgcctt tggcaatcaa cacaaaaata tccaaaagca
     3001 gacagcttgt ttagcagcaa tcttccaatg atgcatttaa tggcaggaac tgcatacttt
     3061 gatgcaacag cagaaaaaaa tgagaattat atttatcgta ttagtttaat tgcatctgat
     3121 ggcaaatcta tttttacaaa agaaagtaat gcaactgctc aatttaaaaa agcatctctg
     3181 ccaaaaataa atttcgaaag ctgcaaatat gcagaaggaa aacttgtttt aacatggagt
     3241 gttaaagacc ctttgagaat gtcacacttc aatgtttata gaacaatatt tggtaaagaa
     3301 gaatatgcaa agatttcaat agagcgaggt gtttattctg aaaacaacaa cctgatgtta
     3361 cttgctattg acactattgg aaataagcct gcatggtatg aatataaaat tgctgctgtt
     3421 gatgcttatg gtaatgaagg cgaactgcaa gccattgcaa gtggaggtag tgtagaagat
     3481 tattatgcac caccaataac aaatctaaaa gcaattaata ccaacagtaa tcatgaggta
     3541 agactttcct ggcgacttga aaaaaagaaa tatctgaatg gaataaatgt aatgcgcagc
     3601 acactgttcg attcaggata tcaaagaatt gcaacattgc cggtgagtga tacttctttt
     3661 acagatatag ttccattatc aggtgaaaac tactattact atctacagat tctaagtgct
     3721 aatgacaaac ctgtttcttc tgcaaaagtg tttgcagctt attccggaga taaatcagta
     3781 cctctgccgc ccaaagaaat tgatgctaca tcacaaccca aagggataaa tgtttattgg
     3841 aaaagtgatg aaccatttgc caaaggattt tatgtgtaca gaagagataa cacaaccgtt
     3901 ccgtttgtat taataagtcc attaattgct tcaaggcaac ctatttactc tttttcagat
     3961 acatcacagc aacttcaagc aggtggtgtt tatcaatata ttgttcgaac catcagtgat
     4021 aacaatcaac tgagcaaacc ttcagataca gtaagtgcta atcccggaat aaaaaaagcg
     4081 atgactccac caactaatct gcgctttaga gaaaataatg gcatgttaac attactgtgg
     4141 gacgacatgc gcaattggga aagtaattta atgggctata aagtgtatcg aaaaaccagt
     4201 aatggtgctt ttgtaaaaat ggataatgac agtttgaaat ccgaacgtaa cttttttgaa
     4261 gattcagaat tatcttcagg aacatcttat gtatatggtg ttaaaacatt tgacaattca
     4321 ggaagtgaaa gtgagatggc aacaattagt tttactgctg tgggtgaatt gttagcaggt
     4381 ccaccttcag gaatttctgt aagccagacc ggtgacaatg tgtttatcac atggggacag
     4441 atttccggag atattgtagc aattaaaatt tataaatctg aaaagggcat acagcccaaa
     4501 ttaattgcaa ctattaccga tgtagattct tatacagata aaaatgtgtt taaagggaaa
     4561 ctgtattttt atcaattatc caccgttggc aaatcaaata aagaaggtgc acagagtgaa
     4621 aaaatttcta tcagggttaa ataatcaact cttcaatcac attctttcgt agagatggca
     4681 aagaattgct gctttattat ttggcacacc acatttgtct gctatagctt taagccttct
     4741 ctttaaagta gaaacagata ttttacaact cactgaaatt tgtttgttac agatatgctt
     4801 tcttaaactg taaataatat tcaattcttc atcagaaaaa tgccctgcta attgaaaatt
     4861 gcggctcagt acagtaagcg atctggcggg tctgccgaat tttgctttac ccttatttat
     4921 tttcctgacg tgatgttgaa gattttctct aatatcatta acaggaatgg tgaggtcaag
     4981 aatttttaag gtgtctgtca catccttttg caataacaga cttgtattac agttcagaca
     5041 atatattgtt tcatgagcat taatattaga tttctgactg agcaaaaaat tcagataatc
     5101 ccatgttctg gattgatgta caaataaaat atcgaaagaa taaaacgatg caatgcttgc
     5161 ttcgctgacg tgtttaatgg ttaaattgtc attgaaagaa ttaagcaatc ttaaaaaagc
     5221 atctgcaaat aaactatcaa cattgaggat gccgatattg aaatgcgtat ttccggtttt
     5281 aagcataata atggttgttt aagtaaagca agtatatgat tttttttgat aactgacaag
     5341 aaaaaaagcg gtaagcatat aaattgagcc aaaagttcgg ggttatgaac attggagcta
     5401 ttcaggcttt ttataatttt gaaaaacaat aaagttggtt agcgttcatg gttgatttat
     5461 ttttatttaa aaatgtaatg tgactttaat attgaagcaa taaatctttt aaaaaaatct
     5521 tcatcacaca ataaaaacaa gaaaatgaaa aagattaaac tcataataat attgctgtta
     5581 gtaagcaaca tgttgcaatc gcaaaccata gtattgcaag aaacttttga tggccaagta
     5641 atgccaacgg gctggcatga agacagtgca ggctttgctc ctgtaattaa tcaatggaat
     5701 tttatttatt acccgtttac aatgaatggt attggtttcg atgcttcatt tatcagaaca
     5761 ggtattgcta atcatgatta cagcttagta tctccttcgt tttcaacagt aggtttatct
     5821 gatgtctatc tttcttatag tgattaccat caaggtccaa ttgatagtgg ttatcagaat
     5881 gtggaaatgt ctgttgacag tggccaaagt tggacaatta tgttatctaa caatggatat
     5941 aatcattatt taggacaaaa tggttcaaga agagtagttc atttaggaac caatgcagcc
     6001 aatcagccca atgtaaaaat tcgttttcat tttaaggtgg gtggtggatg gcgttgggca
     6061 atagacagtg tagtagtaaa cgactatcag ttatgcacta caccaacaga cagcacagga
     6121 gtggtatata gcagcaaaga ttttttatgc tcgggcatag caggtttttt ttggatagac
     6181 catgttagtg gcggcatagg tcaaacctat caatggcagt acaaaccctg gggcagcagc
     6241 agctatttaa acattgccgg tgccgtttac gacaccatga cgtttgttca aaatacgcca
     6301 aactattacc gctgtctggt agtctgcaat gccgatacag caggctctat ggtaaccttt
     6361 gttacagact cgcccacagt ggtacctaag ttaagggcaa caaattttag tggtatttgt
     6421 cccggcagcc ccgatacaac ttatatcttt tgcgaaaaaa atattccagg cataggctat
     6481 caatggcaat ggaaacaaaa cattgcagac ccctttacag atattgcagg agctaacgat
     6541 actttgtatg ccatagatgc agcagtgttt cctaatcaga tatatttacg ctgcaaacag
     6601 caatgtattc aaagcggcaa ataccttgct acagcaacag aggtaatatt tgagaatccc
     6661 aatccaagct gctactgtat tccctacaat caaaactact gcgggccttc taccccaatt
     6721 tttgatggcg gtattgttga tatgcacata gcaggaacaa cgttaaacaa tccgtcttca
     6781 acatgctcaa acatttatgg atatcccggt cagttgagca actacaccta ctttgaccca
     6841 acggttgcaa atcaaacagc aacactgtcg cgcaatgtac cctatagcat gactattact
     6901 acagatacct tacaaatatg gtttaaaggc ggactgtgga tagactacga ccacgacagt
     6961 tattttgaga acacagaata tactaccata gactcattat atcccggcac accaaggacg
     7021 attaccttta gtattccggc cacagccaca cccggcctaa caggaatgcg cctgcgtgta
     7081 gcagggaatt tcccatcagt actgaacgct aacagcgcct gcacttatgc ctatggaggc
     7141 gaaacagaag attatatgat aaccatagac accatagtgg gcattggcga agtaaagcag
     7201 catgaggcta atgtacagct atatcctgtt cctgcaagcg gttatatagt tgtgcaatca
     7261 tcacaaacaa taagaagtgt gaggttagag agcactacgg gagcattagt aaaacaggat
     7321 aacggattaa acacaaacaa ctggcgaatg gatgttagca gctatgcacc gggtatgtac
     7381 ctgatgcgca cagaaacttc tgccggtgta gtgatgaaaa aagtaatgat aagcagggag
     7441 taaaaac
//
LOCUS       DAORQT010000066         7390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1517_length_7390_cov_15.557055, whole genome shotgun sequence.
ACCESSION   DAORQT010000066 DAORQT010000000
VERSION     DAORQT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_7390_cov_15.557055"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PK134_03710"
     CDS             <1..698
                     /locus_tag="PK134_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37985.1"
                     /translation="ERASNKKKMQDDLSEAALMNQTLSDLSALDAADKKKRDEEKDAL
                     AQKEIERASFAADMRVYEYETNKRISDEEVLLRRNQQAEIANILGNAMNVFGRQTIAG
                     KSLAVANALINTYSGATDALRAKSTLPSPFDVIAKVANVATVLATGFKSVKAINSVQI
                     PGGGGGSFSMPTMNASAPLAPQQSSTSLNAGSIQGVGNAAAGGVGRSFVLDADIKSNQ
                     ERQSRIARAARLG"
     gene            733..1326
                     /locus_tag="PK134_03715"
     CDS             733..1326
                     /locus_tag="PK134_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HRB37986.1"
                     /translation="MEYPLFELQISEEEDSALEVTYTALVGEPAIEKNFLAFSEQKQN
                     FAIVDNERRIISGPAMLPDYAMFRKADGDMPDHKVYFKKETIYEIAQKFFAKGFNQNF
                     NLMHNPELKVEGVSVFESFITDSERGIMPMKGFEDVPEGSWFMSAKVNNDEVWEKIKA
                     GEVKGFSVEGFFKYKKPKMTEEAAFEKIKSILNEINE"
     gene            1360..1977
                     /locus_tag="PK134_03720"
     CDS             1360..1977
                     /locus_tag="PK134_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37987.1"
                     /translation="MNRKEAIEKIKKLVFGEEVPTPAPAPAAPAVFGTEYKLADGNSI
                     MIDKLEVGGIAKIGEEVAPDGVHALEDGTSFEIKEGIIVAVTPKEEEKPAEPIEMGKE
                     LEALNKFAENAPAEQQPMVTIVRALFENVFGWQIREAQERATREAAIEVYKTGFEKQK
                     EANQELVKLVEFLAEEPIVKPAQPQKKFEEMTPLERFRFQKEQSN"
     gene            1981..2157
                     /locus_tag="PK134_03725"
     CDS             1981..2157
                     /locus_tag="PK134_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37988.1"
                     /translation="MALKIKEGERIQAYGAGSKAFDENSNLSQAILEHLKSRFPDQIE
                     EIKEIKEHKKEPKK"
     gene            2164..3156
                     /locus_tag="PK134_03730"
     CDS             2164..3156
                     /locus_tag="PK134_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37989.1"
                     /translation="MAISATIVDIRGKAYEPVITELLFENKTVADNLVSFEDDVKANT
                     IFTENSNIVAMQAYTSGAPSSSGTLTLTDTLITPTKLMFYQEFDPNALRSSRFNRDMK
                     PGAWNVASSEFERSVLAAYAKEVSYDAELKFWSGLTAATQTAIAGLSPGVGQAAVGAA
                     EQTWAAAQTPALVDGVIGRMIYNGGALGTRVKVAGTTISSSTIAAEYAKVYAAIPAVV
                     LASGEQPFIYAPHSHKQLINIYNVTATYRDLFSVSGDKYYYNGVEIKFVPLSENVIVA
                     AKPSHLVWCTDLVSDLNYMEINKIANNRDDMFVKHVFTQFTHVVNQAVNVLYVG"
     gene            3202..3723
                     /locus_tag="PK134_03735"
     CDS             3202..3723
                     /locus_tag="PK134_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37990.1"
                     /translation="MACVLTQGYNLDCRDSYGGVKEIYITETSSVNAITASAGVITAI
                     DKTGTKVFFKYNLVAHTAEADQELTPSRENGTTMVKQTIKFPINKMTVAVRNELLLLA
                     QNRLNIVVVDNNGVGWLYGEGNGMMLAPTTAKTGKALGDRNGFELTFESDEKTLAQQV
                     DAATLLTLTTAEV"
     gene            3825..4178
                     /locus_tag="PK134_03740"
     CDS             3825..4178
                     /locus_tag="PK134_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37991.1"
                     /translation="MLILTQGQTADLIVVTLNEKRTQSNPTYVFTATHVTTKEVISFT
                     LGTDLSSYTDRYNEFSINTSVRFLNKPTGQYQYSITQQSGGLEVECGKLVLNPSTEFA
                     FSGYEPETSYNGYNG"
     gene            4141..5997
                     /locus_tag="PK134_03745"
     CDS             4141..5997
                     /locus_tag="PK134_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37992.1"
                     /translation="MSLKHHTMDITDKVISIKFADNRIPEFKEVKGIDYIKFGDDDKF
                     PDHTLMLYNKSAKHNAIVNGKVNYILGGGLICDTPEDPASKLFLQEMSRIDNQSIKDI
                     ELHGGFYWEMIPNKKGGFICYHIDFSRIRSNEDNSIFYYKKDWNDRAEQRKAYPAFKK
                     GLDVPSIFMFKEYRPGLKTYPLPGYVASFNYIEADIEVSKHTLTNAQTGFSASKFINF
                     YNGEPTEENKRDITRRFELLSTGSEGKKVLIGFNNDPAKRPTIDDLGASDLTKEDFSH
                     VDNLITNNIFSGAQITHPLLFGIQQEGKLGGATELRTAYDIFKNTYVNGKQKQYEDVV
                     NHFARLKGVSGTFKLKDVEPVGFDFSESTILQVAPKEWLLEKLGIDPQAYPSTAVAVN
                     SEGLSEQGVNENVKNLSAKQHQQLARIIRQYGKDQLTRQQATILLKTGLGFTDEEVNG
                     LLGDEPTPQAFSSDDELFAAFEAHGEQREGYEVVRTKEATFDSDDEAYELWQAFDDDF
                     INPSTPQTASGGVAVSGKTPPEIKSAFPNFIIRYSYEVRPGEGPELLPTSRPFCQKMV
                     KLDRFYSRQDIQKVSGILGYNVFERVGGYWNDNGVIKKHCRHFWKANIVIKK"
     gene            5994..6575
                     /locus_tag="PK134_03750"
     CDS             5994..6575
                     /locus_tag="PK134_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37993.1"
                     /translation="MSKNTLLIMPRIIKDRTGVHTNIDDKLLYPEIKAAQDMYILPLL
                     GSNLFNKLQNDIEAETLTGDYKDLMDDYIIDCLINYVLAELPDGLSVQFWNRGLSRKT
                     GDDTQPLNMTEMYTVVSKYRNRAEHYAKRCRMYLIQNGTAMFPEYSSVLVGADSIPPD
                     RVSFVSPIYLGDMGIEIKVRDNPGYNSNDPIYL"
     gene            6643..7104
                     /locus_tag="PK134_03755"
     CDS             6643..7104
                     /locus_tag="PK134_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37994.1"
                     /translation="MTLNQVVQRIKTIALAHKQINDFREGDVISFLRSGDIVYPACLL
                     QVLPGRISKAEKQTTVRFALYLCDKVDLSIDSKANELEVKSDLLSIAEDMMAAFDYPT
                     YKLDWDFAEEASIDFLDEDLEDMLSAVLMTLDIKKRFDSNRCQIPTTLTLT"
     gene            7105..>7390
                     /locus_tag="PK134_03760"
     CDS             7105..>7390
                     /locus_tag="PK134_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB37995.1"
                     /translation="MAKIAYPDKQTAVDPNTPASNEIFTAANANEIKVSVNDLYDLEE
                     TTVNTTTTDFTQATLNSTYASAAIGKKVACPNLSGSGPTLYIKITSTVWYS"
BASE COUNT         2495 a         1293 c         1615 g         1987 t
ORIGIN      
        1 atgaaagggc atcgaataag aaaaagatgc aggatgattt aagtgaagca gcacttatga
       61 atcaaaccct ttctgatcta tctgctttgg atgcagccga taaaaagaaa cgtgatgaag
      121 aaaaagatgc tttagcacaa aaggaaatag aacgtgcttc ctttgctgcc gatatgcgtg
      181 tttatgaata tgaaacaaac aagcggataa gtgatgaaga agtactgtta agaagaaatc
      241 agcaggcaga gattgctaat atcttgggta atgccatgaa cgtattcggc agacaaacaa
      301 ttgccgggaa gtctttagca gttgcaaacg ctttgattaa tacttactca ggagcaaccg
      361 atgcactccg ggcaaagagt acactcccct ccccttttga tgtgattgca aaggttgcca
      421 atgttgccac agtattagcc acagggttta aatcagtaaa ggcgattaac tccgtacaga
      481 ttcccggagg tggtggaggt tcattctcaa tgcctacaat gaacgcttct gccccacttg
      541 ctccacaaca atcaagcact tcacttaatg ccggatctat tcagggagtt ggtaatgcag
      601 cagccggggg tgttggtcgt tcctttgttc tggatgccga tattaagagc aatcaggaaa
      661 ggcaatcacg tattgccaga gctgccagat taggctaaac gaaaaatatc aatacttcca
      721 tacactactg ttatggagta cccattattt gagttacaaa tatcagagga agaagattca
      781 gctttagagg tgacctatac tgctttagtt ggtgagcctg caattgaaaa gaacttcctg
      841 gcatttagtg agcagaaaca aaactttgca atcgtagaca atgagcgcag aattatatcg
      901 ggacctgcta tgttgcccga ttacgctatg tttagaaaag cggatggtga tatgcctgat
      961 cataaagtct atttcaaaaa ggaaacaatc tatgagatag cacagaaatt ctttgcaaag
     1021 ggattcaatc agaacttcaa tttaatgcac aatcctgaat taaaagttga gggcgtttct
     1081 gtatttgaat catttattac ggattcagaa cgcggcatta tgcctatgaa aggatttgaa
     1141 gatgttcctg aaggcagttg gtttatgtct gccaaagtaa ataatgatga agtatgggaa
     1201 aagatcaaag ccggagaggt taaaggcttt tcggtcgaag ggttttttaa gtacaagaag
     1261 ccgaaaatga cagaggaagc agcctttgaa aaaataaaat caatcctaaa cgaaataaac
     1321 gaatagaatt atacactatc ctaaaacaac agctttgtta tgaacagaaa agaagcaatc
     1381 gaaaaaataa aaaaattggt ttttggcgaa gaagtaccta ctcctgcacc tgctccggca
     1441 gcaccggcag tatttggtac tgaatacaag ttagcagatg gaaattccat catgattgac
     1501 aagttagaag taggtggtat tgcaaagatc ggtgaagaag ttgcccctga tggtgttcat
     1561 gctttagagg atggtacttc atttgaaatc aaagaaggga tcattgtggc agtaactcca
     1621 aaggaagaag aaaaacctgc tgaaccaatt gaaatgggta aggaattaga agccttaaat
     1681 aagtttgctg aaaatgcccc ggcagaacaa cagccaatgg ttacaatcgt aagagcattg
     1741 tttgaaaatg ttttcggatg gcaaattcgt gaggctcagg aaagagcaac aagagaggca
     1801 gcgattgaag tttacaaaac aggatttgaa aagcaaaaag aggctaatca agaacttgtg
     1861 aagcttgttg agttcttagc agaagagcca atcgttaaac ctgcccagcc tcaaaagaaa
     1921 ttcgaggaaa tgacaccgct cgaaaggttt cgtttccaaa aagaacaaag taattaagat
     1981 atggcactga aaataaaaga aggcgaaaga attcaggcat acggtgcagg aagtaaagcg
     2041 tttgatgaaa acagtaacct atcacaggca atacttgaac acttaaagtc aagattcccg
     2101 gatcagattg aagaaataaa agagattaaa gaacataaaa aagaacctaa aaaataaaaa
     2161 gcaatggcaa taagtgcaac aattgtagac atcagaggta aggcatatga gccagttata
     2221 accgaacttt tgttcgagaa taaaaccgta gctgataatc ttgtatcatt tgaagatgat
     2281 gtaaaagcaa atacaatctt cactgaaaat tcaaacattg tagctatgca ggcttacact
     2341 tcgggtgcgc cttcatcttc aggtactctt actttaacag acactttgat cactccgaca
     2401 aagttaatgt tctatcagga atttgatccg aatgccttgc gttcttcacg tttcaatcgt
     2461 gatatgaagc ctggagcatg gaatgtagct tcaagcgaat ttgagcgttc agtattggca
     2521 gcttatgcaa aggaagtttc ttatgatgca gaattgaaat tctggtcagg acttaccgca
     2581 gctactcaga ctgcaatcgc aggattatct cctggagttg gtcaggcagc agtaggcgca
     2641 gcagaacaga cttgggcagc agcacaaact ccagctttag ttgatggggt tattggtcgt
     2701 atgatctata atggtggcgc acttggaacc cgtgtaaagg ttgcaggaac taccatttca
     2761 agctcaacta ttgcggctga atacgcaaag gtttacgcag ctatcccggc agttgttttg
     2821 gcaagcggtg agcagccttt tatttacgct ccacattctc acaagcagtt gatcaacatt
     2881 tacaatgtta ctgctactta cagggatctg tttagtgtgt caggagataa gtactactac
     2941 aatggtgtag aaattaagtt tgttccactt tctgagaatg tgattgtcgc tgcaaagcct
     3001 tcacacttag tttggtgtac tgacctggta tctgatctga actacatgga gatcaataag
     3061 attgcaaaca atcgcgatga tatgttcgtg aagcacgtat tcacccaatt cactcacgta
     3121 gtgaatcagg cagtaaacgt tctttacgta ggataattta aggggagggt aatacctccc
     3181 ttaaccttta aaatataaaa aatggcttgc gtacttacac aaggttataa tttagattgc
     3241 cgggatagtt acggaggtgt aaaggaaatt tacataactg aaacatcaag cgtaaatgct
     3301 atcactgcat cagcaggagt aattacggca atcgacaaaa caggcacaaa ggtatttttt
     3361 aaatacaatc ttgtggccca tactgcagaa gctgatcagg aacttactcc ttccagggag
     3421 aacggtacta caatggttaa gcagacaatc aaattcccga tcaataaaat gactgtggca
     3481 gttcgcaatg aattgttact tcttgctcag aaccgtttaa atatcgtagt ggtagataat
     3541 aatggagtgg gttggttata tggtgagggt aacggaatga tgcttgctcc taccactgcc
     3601 aaaacaggaa aggctcttgg agatcgtaat ggcttcgaat taacattcga aagtgatgaa
     3661 aagactttag cgcagcaagt ggatgcagct acattattaa cattgacaac agcggaagta
     3721 taattcagtt ttcatgggtg gttaattagg cggaagccgg gcagaaatgt tcggcttttt
     3781 ttattgtaac gaaaaaaaac acccctttcc tacactattg ttatatgcta atcttaactc
     3841 aggggcaaac ggcggattta attgtagtta ctcttaatga aaagagaact caaagcaatc
     3901 ctacttatgt ttttacggct actcacgtaa caactaagga ggtaatcagc ttcacgttag
     3961 gcactgattt aagcagctac acagatagat acaatgagtt ttctataaac acatcagttc
     4021 gatttcttaa caaaccaacc gggcagtatc agtacagtat cactcagcag agcggaggtt
     4081 tagaagtgga gtgtggcaag ttagtattaa acccatcaac agaatttgca tttagtggct
     4141 atgagcctga aacatcatac aatggatata acggataaag taatttctat aaagttcgct
     4201 gataatcgca tccctgaatt taaggaagtg aaagggatag attacatcaa gtttggtgat
     4261 gatgataagt tcccggatca tactttgatg ctttataata agagcgcaaa gcataatgca
     4321 atcgtcaatg gtaaggtaaa ttatatctta ggcggtggtt tgatttgcga tactcccgaa
     4381 gaccctgcat ctaaactgtt cctgcaagaa atgtcacgta ttgacaatca gtcaatcaaa
     4441 gatattgaac ttcatggtgg tttctattgg gaaatgatcc cgaataagaa aggcggtttt
     4501 atctgttatc atattgactt ttcacgtatc cgatcaaatg aagataattc aattttttat
     4561 tataaaaagg attggaatga tagggcagaa cagcgcaaag catacccggc atttaaaaaa
     4621 ggcttagacg ttccttcaat tttcatgttt aaggaataca gacctggatt gaagacatat
     4681 cctttaccgg gttatgtagc atcatttaac tacatagaag cagatattga agtatcaaag
     4741 catactttaa ccaatgctca gaccgggttt tctgcatcta agtttatcaa cttttacaac
     4801 ggtgagccta ctgaagaaaa caaaagagat attacaagaa gatttgagtt attaagtaca
     4861 ggatctgaag gtaaaaaggt tttaattggc ttcaataacg accctgcaaa aagacctacc
     4921 attgatgatt taggagcctc agatttaacg aaagaggatt tcagtcacgt agataacctg
     4981 atcacaaata atatcttctc aggtgcgcaa attacgcatc ctttactatt cggtattcag
     5041 caggagggaa aactcggagg cgcaaccgaa ttacgtactg catacgatat tttcaagaat
     5101 acctatgtaa acggcaagca gaaacaatat gaagatgttg taaatcactt tgcaaggtta
     5161 aaaggtgtat ctggcacgtt taaattaaaa gatgttgagc cggtagggtt tgacttttcg
     5221 gaatctacta ttttacaagt ggctcctaaa gaatggttac ttgaaaaatt aggtatagat
     5281 ccacaggcat acccatcaac agccgtagcg gtaaatagtg agggactttc agagcaaggt
     5341 gtaaatgaaa acgtaaagaa tctatcagca aaacagcatc agcaattagc ccgaattatc
     5401 aggcaatacg gtaaagatca gttaacaagg caacaagcta caattttatt aaagaccggg
     5461 ttaggcttta cagatgaaga agtgaatgga ctattaggtg atgagcctac accacaggca
     5521 ttcagttctg atgatgaact atttgcagcg tttgaagcac atggagagca gagagaaggt
     5581 tatgaggtag tgagaactaa ggaagcaacc tttgattcag atgatgaagc ctatgaatta
     5641 tggcaggctt ttgatgatga ctttatcaat ccatctactc cacaaactgc atccggtggt
     5701 gtagcggtat cgggcaaaac tcccccggag attaagagtg ccttcccaaa tttcataatc
     5761 agatattctt atgaggtaag acctggagag ggtcctgaat tactacctac ttcaagaccg
     5821 ttctgccaaa agatggtaaa gttagatcgg ttctactcca gacaggatat tcaaaaggta
     5881 agcggaatat taggttataa tgtttttgag cgtgtcggtg gttattggaa cgataacgga
     5941 gtgattaaaa agcattgcag acatttttgg aaagcaaaca tagtgattaa gaaatgagca
     6001 agaacacgct tttaattatg ccccgaatta tcaaggatag aacaggggtt catacaaaca
     6061 tagatgataa gctattatac cctgaaataa aagcggctca ggatatgtat atcttgccgt
     6121 tattaggctc aaatcttttc aataagttac agaacgacat cgaagctgaa acgcttacag
     6181 gagattataa agacttaatg gatgattata tcattgattg cctgataaac tacgttttgg
     6241 cagagttgcc tgatggactt tcagtacagt tttggaacag aggattaagc agaaagaccg
     6301 gggatgatac ccagcctttg aacatgactg aaatgtatac cgtagtatca aagtacagga
     6361 acagggcaga gcattacgct aaacgatgca gaatgtattt gattcaaaat ggaactgcta
     6421 tgttccctga atacagcagc gttttggttg gtgcagattc aataccgcct gatagggttt
     6481 cttttgtaag ccctatttat ttaggcgata tgggtattga aattaaggta agggataatc
     6541 ctggatataa ttcaaatgac ccgatttatt tataaggtat gccaaagaat tacagcaaaa
     6601 aaaatgaggc aaaattaagg ttctttttag aaaagaaagc aaatgacact gaatcaagta
     6661 gtacagagaa taaaaacaat agcactggca cacaaacaga taaacgactt ccgggagggg
     6721 gatgtgatca gttttctaag aagcggtgat attgtttacc ctgcttgttt gcttcaggta
     6781 ttgccgggca ggattagcaa ggctgaaaaa cagactactg taagattcgc attatatcta
     6841 tgtgataagg tggatttatc cattgattca aaagcaaatg agttagaagt gaagtcagat
     6901 ttgctttcca tagctgagga tatgatggct gcttttgact acccgacata taagcttgat
     6961 tgggattttg cagaggaagc aagtattgat tttctggatg aagatttaga ggatatgctt
     7021 tcagcagtcc ttatgacatt ggatataaaa aagaggtttg attctaacag gtgtcaaatc
     7081 cctacaactt taacacttac gtaaatggct aaaatagcat atccagataa acaaactgca
     7141 gtagatccga ataccccggc atccaatgaa atcttcacgg ctgcaaacgc caacgagatt
     7201 aaagtatcgg tgaatgatct ctatgattta gaggaaacaa ctgtaaatac aactactacg
     7261 gatttcacac aagcgacttt aaattctact tacgcttcag cagccatagg taagaaagta
     7321 gcctgcccga atttatcggg atcaggaccg acattataca ttaaaatcac atcaacagta
     7381 tggtattcaa
//
LOCUS       DAORQT010000067         7370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1526_length_7370_cov_7.612850, whole genome shotgun sequence.
ACCESSION   DAORQT010000067 DAORQT010000000
VERSION     DAORQT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_7370_cov_7.612850"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK134_03765"
     CDS             <1..778
                     /locus_tag="PK134_03765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRB37996.1"
                     /translation="LSRHPDRPYSLDYINAITDNTFIELHGDRNVKDDKAMIGGFGTV
                     DGRTIMFVGQQKGHNTKSRQYRNFGMPNPEGYRKALRLFKLAEKFNKPIVTFIDTPGA
                     YPGLEAEERGQGEAIARNLYEMSILKVPVICIIIGEGASGGALGIGVGDKVMMLENTW
                     YSVISPESCSSILWRNWDNKEKAANALKLTANDMLDLKLIDGIIKEPAGGAHSNPEEI
                     FATVKKEINVQLGALLTVSDADRVNNRIDKFCSMGVVRKN"
     gene            complement(841..2841)
                     /locus_tag="PK134_03770"
     CDS             complement(841..2841)
                     /locus_tag="PK134_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRB37997.1"
                     /translation="MTHKHIYDSSGKQLCCTLEDNIYENAGRVDLKENHDHNDHDHTH
                     HESGMFSIYLPGLVSLVLLLAGMHFDYWNTFSWFNGWIRLVWYIIAYIPVGFPVIKEA
                     YIGITKGNIFSEFFLMTIATVGAFAIKEYPEGVAVMLFYAVGELLQTMAVRRAKSNIK
                     TLLDQRPDVATVLRNSNTVSVDANDVGIGEVILLKPGEKLALDGILLSDNGTFNTAAL
                     TGESMPDAKVKGASVLAGMINLNTVVKIKVTAAYEDSKLSKMLELIQNASAQKAPTEL
                     FIRKFAKVYTPIVVGLAVAITLLPYFFAHEYIFRDWFYRALIFLVISCPCALVISIPL
                     GYFGGIGAAGRNGILVKGSNYLDVLASVQHVVMDKTGTITEGVFKVQEALIFPEENRN
                     KILQMVNALEKHSTHPVATAIHNYVSNTSTIELEKVEEISGHGMKAFVNGKELLVGNF
                     KLLSLHNISFNVDPATIKHTVIAIAFDRKFAGYIIIADSIKTDAAAAIQKLKQMHVDV
                     TMLSGDKTTVVNSVAEQTAISHAYGELLPEDKVEKVKSIINQKQTVAFVGDGVNDAPV
                     IALSHVGIAMGGLGSDTTIETADVVIQDDNPIKIPLAINIGKQTKKVVWQNITMAFVI
                     KAIVLILGSGGLATMWEAVFADVGVALLAIINAMRLLKMRFD"
     gene            complement(2889..3320)
                     /locus_tag="PK134_03775"
     CDS             complement(2889..3320)
                     /locus_tag="PK134_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRB37998.1"
                     /translation="MLHSNIQTENILVARNTRPTSMRILVYNFLAKQQAALSITEIEK
                     HFQHADRITIYRTLKTFEEKGIIHSIQEDNITKYTLCGSACSEHTHYDQHLHFYCKSC
                     KQTTCKTDIAIPLNNITGFKIDDITFFARGICEKCINPTLQ"
     gene            complement(3348..>3591)
                     /locus_tag="PK134_03780"
     CDS             complement(3348..>3591)
                     /locus_tag="PK134_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB37999.1"
                     /translation="IQRVLKEHRMLADLCKSELELATNSKLELLEKTLEAHIRFEERV
                     LFKEIESIATSEQLELINKHHKEEKFVDNLSDPFWK"
     assembly_gap    3592..3615
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3616..3820)
                     /locus_tag="PK134_03785"
     CDS             complement(<3616..3820)
                     /locus_tag="PK134_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB38000.1"
                     /translation="MSTKPIKRHEAIQSISREHYQGLLFGSKIRMGLKKQIEPDRMAK
                     YAVWFYKEHLIPHFEIEEKYLFPV"
     gene            complement(3817..4170)
                     /locus_tag="PK134_03790"
     CDS             complement(3817..4170)
                     /locus_tag="PK134_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRB38001.1"
                     /translation="MSSVKIKRIYEAPDKSDGYRMLIDRLWPRGVKKENAAIDEWNKD
                     IASSSELREWFGHDPEKFKEFTARYTKELHNKNDELKQIAGIAKKQNLTLLYGAKDTE
                     HNQAVVLQKVLSKMI"
     gene            complement(4276..4857)
                     /locus_tag="PK134_03795"
                     /pseudo
     CDS             complement(4276..4857)
                     /locus_tag="PK134_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
     gene            complement(4985..6436)
                     /locus_tag="PK134_03800"
     CDS             complement(4985..6436)
                     /locus_tag="PK134_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRB38002.1"
                     /translation="MNIDSLKLKKISALWIIVILVLFIASIVLGILMRLNQGKVIEQS
                     PLTFYTNMTTHGLTMIGIWFAAGMVAVNYLLQRYVKTSYGVNLFAFILTVIGVVMLWV
                     TTYAGKFSTGWTFLYPLPFRDLGSESWSTPLFLISLTILGVGWLVWSVSLMAQIFKKY
                     TLAQAFAWQHFKSNPTVETPPFILITTVTLVGVIASLIAAVIVIVLYFAEYFSHGDFV
                     NDALLMKNLIYFFGHTIANEMLYLGLAVIYEIFGEVSDRPKWKMTWYISLAWNCAMVF
                     ILTAFFHHLYMDFVQPQALQIVGQLASYLASIPSAAVTVFSIVAAVYGVKVRWSIVNL
                     LFFLGVAGWVIGGLGAVIDATISNNIMLHNTLWVPAHFHTYNAMGNVLFSIGFFYWFS
                     TQYVDKLVLSKLTKYTLTLLIVGGIGFLMAFYVAGANSIPRRYSTYPVELTSGPYLAK
                     VGALFATIYLIAILIFFYNIAKRCFVILSASSR"
     gene            complement(6452..6928)
                     /locus_tag="PK134_03805"
     CDS             complement(6452..6928)
                     /locus_tag="PK134_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HRB38003.1"
                     /translation="MSSLKADNDSTGVKNLLKKRFWFTLILIVVLGIFAAATLPKSPY
                     FTYANETPSTVIHVGTMQFSFFLSTKPIDPKSPSGETIEIPHDQLVEFRVTSFDVNHG
                     FAVYDPSMRLITQVQAMPGYVNNLRYKFTEPGEYHIFCLEFCGTGHPVMQTTFKVI"
BASE COUNT         2394 a         1316 c         1272 g         2364 t
ORIGIN      
        1 attaagtcgt cacccggaca gaccgtattc tctcgattat ataaatgcta ttaccgacaa
       61 tacttttatt gaattgcatg gtgacagaaa tgttaaggac gataaagcaa tgataggagg
      121 ttttggtact gttgatggaa gaacaattat gtttgttggt cagcaaaagg gacacaatac
      181 aaagtccagg caatatcgta acttcggtat gcccaacccg gaaggttata gaaaagcatt
      241 acgtctgttt aaacttgctg aaaagtttaa taagcccatt gttactttca ttgacactcc
      301 gggtgcctat cccggacttg aagcagaaga acgcggacag ggtgaagcta ttgctcgcaa
      361 tctttatgaa atgtcaattt taaaggtgcc tgttatttgt attatcattg gcgaaggtgc
      421 atccggtggt gcattaggaa ttggtgtagg cgataaagta atgatgcttg aaaacacatg
      481 gtactctgta atttcgcctg agtcatgttc cagtatttta tggcgcaact gggataataa
      541 agaaaaggca gccaatgcgc ttaaacttac tgccaatgac atgctcgatt taaaattaat
      601 tgatggcatt attaaagaac cagcaggtgg tgcacatagt aatccggaag aaatttttgc
      661 aaccgtaaaa aaagaaatta atgttcagtt aggtgcctta cttactgtat ctgatgctga
      721 cagggttaac aacagaattg ataaattctg ttctatgggt gttgttcgaa agaactaaaa
      781 acatttttta ctgattaatt ctttctaaat aggtttatat tattggctga ttcgttaata
      841 ctaatcgaat ctcattttta ataaacgcat tgcattgatg attgctaata gtgcaacacc
      901 aacatctgca aacactgctt cccacatagt tgccaatcca cctgagccta atattaaaac
      961 tattgctttt attacaaacg ccatagtgat attttgccag accactttct ttgtttgctt
     1021 tccaatattt attgctaaag gaattttaat cgggttgtca tcctgaatta caacatctgc
     1081 agtttcaata gtagtatcgc tgccaagtcc acccattgca ataccaacat gacttaatgc
     1141 aataacgggt gcatcattaa caccatcacc aacaaaagct actgtttgtt tttgattgat
     1201 tatggattta actttttcaa ctttatcctc aggcagtaac tcaccataag catgagaaat
     1261 tgctgtttgc tcagcaactg aatttacaac agttgtttta tcaccactca acatcgtaac
     1321 atctacatgc atctgtttta atttttgtat ggcagcggca gcatcggttt taatgctatc
     1381 tgcaatgatg atgtagcctg caaattttct atcaaaagca atggcaatga cggtatgctt
     1441 tatggtagca ggatcaacat taaatgatat gttatgtaag gataaaagct tgaaatttcc
     1501 tactaacaac tctttgccat taacaaatgc tttcattcca tgacctgaaa tttcctctac
     1561 cttttccagt tcaattgttg aggtatttga aacatagttg tgaatggctg ttgcaacagg
     1621 atgtgtactg tgtttttcaa gcgcattaac catttgcaaa attttatttc tattttcttc
     1681 cggaaaaatt aaagcttcct gaactttaaa tacgccttca gtaattgttc cggttttgtc
     1741 catcacaaca tgctgcaccg atgcaagtac atctaaataa ttactgcctt taaccaaaat
     1801 accattccgg ccggcagcgc caatgcctcc aaagtatcct aaaggaatac ttattaccaa
     1861 tgcacaagga catgatatga ctaaaaatat taaagctctg taaaaccaat cacgaaaaat
     1921 atactcatga gcaaaaaagt agggcaataa agtgatggca acagcaagtc caacaacaat
     1981 aggggtataa acttttgcaa attttctgat gaataattca gtgggagctt tctgtgcaga
     2041 tgcattttga attaattcaa gcatcttgct caacttgcta tcttcataag cggctgtgac
     2101 tttaattttt acaacagtat taaggttaat catgcctgca agaactgaag ctcctttcac
     2161 ttttgcatcg ggcatactct ctcctgttag tgcagcagtg ttaaaagttc cgttatcaga
     2221 aagtaaaata ccatcaagtg ctaacttttc gcctggtttt aaaagtatta cttcgccaat
     2281 gccaacatcg ttagcatcta cagaaacagt gttactattt cgtaatactg ttgcaacatc
     2341 aggacgttga tcaagtaagg ttttaatatt tgattttgct cttcttacag ccattgtctg
     2401 aagtaattct cccacagcat agaataacat tacagcaaca ccttcaggat attcttttat
     2461 tgcaaatgca ccaacggtag ctattgtcat cagaaaaaac tcagaaaaaa tatttccttt
     2521 tgtaattcca atataagctt ccttgattac aggaaatcca accgggatat aggctattat
     2581 ataccaaact aaacgaatcc acccattaaa ccaggagaag gtattccaat aatcaaaatg
     2641 cattcctgcc agaagaagca ctaaagaaac taatccgggc agatatattg aaaacatacc
     2701 ggactcatga tgcgtatggt catgatcatt gtgatcgtga ttttctttca aatcaacacg
     2761 acctgcattt tcataaatat tatcttcaag agtacaacaa agttgttttc cgctggagtc
     2821 gtaaatgtgt ttatgcgtca tgccatatat tttctgaata caaaattatg gcagatgtca
     2881 atgcaatcct attgcaaagt cgggttaatg catttttcgc aaatacctct tgcaaaaaat
     2941 gttatatcgt caattttaaa tccggtaatg ttgtttagcg gaatggcaat atcggttttg
     3001 caggtagttt gtttgcaaga cttacaataa aagtgaagat gttgatcgta gtgggtatgt
     3061 tctgaacaag cagatccaca taaagtatat ttggttatat tatcttcctg aatgctatgt
     3121 ataatgcctt tctcctcaaa tgtttttaaa gtacgataga ttgtaattct gtcagcatgc
     3181 tgaaaatgtt tctcaatctc agtgatagat aatgcagctt gttgttttgc aaggaaattg
     3241 taaactaaaa tacgcatgct tgtcgggcgt gtatttctgg caactaaaat attttctgtt
     3301 tgtatgtttg agtgcaacat gttgttcatt gaaattatga gtaggtgtta cttccagaat
     3361 gggtcagata aattgtcaac aaacttttcc tctttatgat gtttgtttat cagttcgagc
     3421 tgttcagatg tagcaataga ttcaatttct ttaaagagta ctctctcttc aaagcgaatg
     3481 tgggcttcca atgttttttc gagtaattca agtttgctgt ttgtagccaa ttccaattct
     3541 gatttacata aatcagcaag catgcgatgt tctttaagta ctctttgaat cnnnnnnnnn
     3601 nnnnnnnnnn nnnnntaacc ggaaataaat atttctcttc tatttcaaag tgaggaatga
     3661 gatgttcttt ataaaaccaa acagcgtact ttgccattcg gtcaggctct atttgttttt
     3721 taagccccat cctgatttta gaaccaaaaa gtaatccttg ataatgttct ctgcttatgg
     3781 actgtattgc ctcatgtcgc ttaattggtt tggtgctcat atcattttac tcaatacttt
     3841 ttgtaaaact acagcttggt tgtgctctgt atcttttgcg ccataaagta acgtcaagtt
     3901 ttgctttttt gcaattccgg ctatttgctt taattcatca ttcttgttgt gaagttcttt
     3961 tgtataacga gctgtaaatt ctttgaattt ttcagggtcg tgaccaaacc attctcttag
     4021 ctctgaagat gatgcaatat ctttattcca ttcatcaatg gcagcatttt cttttttaac
     4081 acctcttggc catagcctgt caatcaacat tctataacca tcacttttgt ccggtgcttc
     4141 ataaatccgt tttattttta ctgatgacat aactgtttta atttttaaac ttcatatcag
     4201 aaaacaaaat tgcattgatt atggttttat agttccgata atttatttta agtgacaaaa
     4261 agtgtgttta aaattctaag aaactgttct ctttgattta ataacaatta ttgtaacaat
     4321 aagaaaagtt atgattgcag gcaaggctac aaataaaagt cctaagccag gtctgtttgt
     4381 ttgtgttttc ggatcgtagt taaagcatga aaatatatta ctttttgttg gtaaacggta
     4441 ggagggagta aatacattat taatgctgct tatcatttta tgtacgtctt ccacaattct
     4501 gattcccaaa agatttttag ttataagccc tttctcattt acaccaacca gcaaagcatc
     4561 gtggtcaaac tgtttgataa cgctatccca tttcggaata aatcctatgg aattaataaa
     4621 tgcatcaata ctatcggtta ttgcaaagtt ccattgaaga ttattctcaa ggccatattg
     4681 ctttgaaaga ttagtcatat cctgaaggtt atctcttgga tcaaaactta taactaaaat
     4741 attaaaatct gaattgcttt gtagatgctt tatattttcg ctcaattgaa gtaagaatgg
     4801 attgcagatg ccataacatc ttgtaaaaac taaagctatg attaatggtt tctgattata
     4861 taattcttca acagaagata ctttattatt ccgagtggtg cgttataaat ttttccgtaa
     4921 agattctgct cttccctgaa gtcagagtga ttatcattag cataacctaa aaagctaaaa
     4981 catgttagcg cgaagaagca gacaaaataa cgaaacatcg tttagcaata ttataaaaga
     5041 atatcaaaat agcgataaga tatattgttg caaacaaagc acctacctta gcaagataag
     5101 gccctgatgt gagttcaaca ggatatgtac tatatcttct tggaattgaa tttgcaccgg
     5161 ccacatagaa tgccattaag aagccaatgc caccgacaat tagaagtgtt aaagtgtatt
     5221 ttgtaagctt acttaaaact aatttgtcaa catattgcgt agagaaccag taaaagaaac
     5281 cgatgctaaa caaaacgttt cccatggcat tgtaggtatg aaagtgtgca ggaacccaaa
     5341 gcgtattatg cagcataata ttatttgaaa tagttgcatc aatgacagcc cctaagccac
     5401 caattaccca tccggcaaca ccaagaaaga acaataggtt tacaatagac catcttacct
     5461 taactccata aactgctgct acaatactga aaaccgtcac agcagcagat ggaatacttg
     5521 caagatatga ggccaattgt ccaacaattt gcaaagcctg tggttgcaca aagtccatgt
     5581 ataggtgatg aaaaaatgca gttaaaataa ataccattgc acaattccat gccaatgaaa
     5641 tataccaggt cattttccat tttggcctgt cgcttacttc tccaaaaatt tcatagataa
     5701 cagccaagcc taaataaagc atttcgtttg caatggtatg accaaagaaa tagatcaggt
     5761 ttttcatcag caaagcatca ttgacaaaat ctccatgaga gaaatattct gcgaaataca
     5821 atacaataac aattactgct gcaattaacg aggcaatgac accaactaat gtcactgttg
     5881 ttatcaatat aaaaggtggt gtctctacag ttggattact tttgaaatgt tgccatgcaa
     5941 aggcttgggc caatgtatat tttttaaaaa tctgagccat cagactaaca ctccacacca
     6001 gccatcctac acctaatatt gttaacgaaa taagaaacaa cggtgttgac caactctccg
     6061 aaccgagatc tctgaatggt aatggataga gaaaagtcca gcctgtgcta aacttacctg
     6121 cataggtagt tacccaaagc attacaacac caataacagt aagaataaag gcaaacaagt
     6181 taacaccata acttgtcttc acataccgtt gtaaaagata gtttacggca accattcctg
     6241 cagcaaacca aatcccaatc atggttaaac catgtgttgt catgtttgta tagaaagtaa
     6301 gtggcgattg ttcaataact tttccctgat tgagcctcat aagaattcca agtacaatac
     6361 ttgcaataaa aagtacaaga atgacaatta tccaaagtgc gcttattttt ttaagcttaa
     6421 gtgaatctat attcatgatt taattttttt attaaataac tttaaatgtt gtttgcataa
     6481 cgggatgccc tgtgccacaa aactcaaggc aaaaaatgtg atactcgcca ggttcagtaa
     6541 atttatagcg taggttgttg acatagccgg gcattgcctg tacctgagta atcaatcgca
     6601 ttgaaggatc gtaaacagca aatccatgat tgacatcaaa acttgttact ctgaattcta
     6661 cgagttggtc gtgcgggatt tcaattgttt ctccacttgg acttttaggg tcaatgggtt
     6721 tagtggaaag aaaaaatgaa aattgcattg tgccaacatg aatcacagtt gaaggtgttt
     6781 cattcgcata agtgaagtat ggggattttg gtaaagttgc tgcagcaaaa atgccaagta
     6841 caacaatgag aattaaggta aaccaaaaac gtttttttaa caggttcttt acacctgttg
     6901 aatcattgtc agctttgagt gaagacataa agattgacac aaaaaatata gtgccaatac
     6961 caacaagcag cataaaaaat aacataatgg aatgttgttg cataactttt agtttaaata
     7021 attaaagtga atgtgatgta tatgatgatt aagattaact tgtataggtt tgttaatccg
     7081 aattcctgaa tgaataaatt gagtagagaa aagttgtaga tttttgaaga atctatgaca
     7141 ttttttacct cttaaaatgg ggcaaagcat tgccatcaag ccagcggaat tttggttgcc
     7201 tccaaaaaca atttcccgtt gatgtaatat aaccttttta tgggttacag gtgcataaaa
     7261 tataaaacat gctaccatgt atccgttaaa ctacattagg tagtgcaaac gtataaacta
     7321 tttcttagga atccaaaaga agtatataaa tacttttata acatttatgt
//
LOCUS       DAORQT010000068         7298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1549_length_7298_cov_7.073036, whole genome shotgun sequence.
ACCESSION   DAORQT010000068 DAORQT010000000
VERSION     DAORQT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1549_length_7298_cov_7.073036"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1133)
                     /locus_tag="PK134_03810"
     CDS             complement(<1..>1133)
                     /locus_tag="PK134_03810"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38004.1"
                     /translation="VLTPLSSFSSVANPADIKIVHADNIYYAFIASKGNGNVTCLSFG
                     NSMSNLPLELYSFNITGSINLSDIDIVTTCAPPVGFVTDSKNGDLTKLSFSSGFNNAP
                     SLSVYSGTGSIGNNSVTITQEFESYFVCFTDTANKQLKWLQFNENNFANTPTVYTSPA
                     FYFRPNQAESLTDSSITRFIVSDVPTLNFVDMLYFDSCYTKYSEDQNPSFSYLNTGWN
                     KLSLTVSDSNGYKSIAYDSVFVITGPKSQFNFTGNTCFNPNDSISFTDQSTSPSGAIT
                     GWLWDFGDGTTSTQQNPQHAYTASGTYQVKLTITAGCEKDTIIPITIHALPIINFTYN
                     LSCANSATTLTDQSSSAAGLQSWTWNFGDGSSSSQLQNPDHIF"
     assembly_gap    1134..1152
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1153..1632)
                     /locus_tag="PK134_03815"
     CDS             complement(<1153..1632)
                     /locus_tag="PK134_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38005.1"
                     /translation="MQKIFFALLLLFSLSRNAQSQCPTASFSLPDTVCSGIPLPLTNT
                     STGNGLTYQWDFCPQEIHGFTSYQYFRYNNYVLYTMTDMDLLKVGSGYYLITMDSIYN
                     NFVVGNIGNHLSNRPFDAIVHGNFTKLDCFDFIVEGDTNVYGLAAYSPTSTLYLMTFP
                     "
     gene            1684..2268
                     /gene="rdgB"
                     /locus_tag="PK134_03820"
     CDS             1684..2268
                     /gene="rdgB"
                     /locus_tag="PK134_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRB38006.1"
                     /translation="MTELIFCSNNEHKLQEIRKMLPFGYILQSLSEAGIQSNIDETGT
                     TFLENAFLKANAVSLLTKKNCFADDSGLCVAALNNAPGVYSARYAGNEVTDADNRRKL
                     LSDLSGIQQRNAYFIAVICLIWKGKAFYFEGRIDGMITHIEQGNSGFGYDPVFIPDGY
                     KITFAEMSPDEKNKISHRQIAVSKMIAFLRNSKV"
     gene            complement(2258..3658)
                     /locus_tag="PK134_03825"
     CDS             complement(2258..3658)
                     /locus_tag="PK134_03825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB38007.1"
                     /translation="MNITFYGAARNVTGSKHLIETESGKKILLDCGFFQNRGKDNDRL
                     NRNFSFDPQQIDLMLLSHAHIDHSGNIPNLVKQGFNKTIFTTQATIDLCEVMLADSAY
                     IQSGDIEYINRRRKRDGQKALEPIYEIEDVEKAMKLFAPVAVNKRFHYDDEISFEFTD
                     AGHILGSVSVHLFITEKGNTKQITFSGDVGRFNDLILKAPASFSQADYILCESTYGNR
                     LHDQSTDARQKLLQIVNKTCVEKKGKLIIPAFSLGRTQEIIYTLDRFKTENKLPSIPV
                     FVDSPLAIDATNIMRKHSEFFNSDLVNYLHVDDDPFGFSNLKYVRKVEESKKINDLKG
                     PCIIISASGMIEAGRIKHHIKNNINDSRSTILIVGYCTPESIGGHLMRGDKTIKIFGN
                     EYLVKASVEVISSFSAHADYLELIKYLSCQDTSAVKKLFLVHGEYEVQKEFKEKLNEV
                     GFKNVEIPEELQSFKL"
     gene            3769..4407
                     /locus_tag="PK134_03830"
     CDS             3769..4407
                     /locus_tag="PK134_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRB38008.1"
                     /translation="MSFIQYFIPQDKKFFPLFDQAALNLQEAGKTMCQLVTSTDADQR
                     KQLIREIERLEHRGDEITHEIFKELSRNFITPFDREDIHRLVSSIDDILDYIHGSSKR
                     IDLYKVKEFSSDMVKLSELLQTQTEELRRVIYELKNKKNMRNISESLVLINSIENHAD
                     DIFDNAVARLFETETNAVEIIKTKEILSALETATDMCEDAANVIDSIIVKMA"
     gene            4412..5404
                     /locus_tag="PK134_03835"
     CDS             4412..5404
                     /locus_tag="PK134_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRB38009.1"
                     /translation="MTHALIAIIILALLFDFINGFHDAANSIATVVSTKVLSPLTAVI
                     WAASFNFLAYWIFKLKVADTVAKTVTPDSVTLMVVGAGLVAAIIWNLLTWWWGIPSSS
                     SHTLIGGFAGAGIANAGSFSALQVDKVMPTVYFIILAPIIGMVMSWIISVITLHLCRK
                     SNVSQVDRTFRKLQLLSSAAYSLGHGGNDAQKVIGIVGAAMLSHGMISDIDHIPEWVP
                     LSCYSAIALGTMFGGWRIVKTMGQRVTKLTPFEGFSAEAAGAFTLFFTQHFKIPVSTT
                     HTITGSIIGVGLTKRISAVRWGVTLQLIWAWILTIPVSGALAAIIFMIARKVFS"
     assembly_gap    5423..5461
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5668..6201
                     /locus_tag="PK134_03840"
     CDS             5668..6201
                     /locus_tag="PK134_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRB38010.1"
                     /translation="MANTKFIFQALHVIAWIIFVGLCIEAGGIIVNFIFSIYNPEFVS
                     KLYQKLDLSELYNSNKWIFYSMYSFIIVIALLKVFLFYTVIMLLLKLDLSKPFSSFVS
                     TQISQISYYTLSIGIICLIAAHTTKNLEHHGYVVERLFQFWNDGQAFILMSAVVYVIA
                     TIFKKGVELQNENDLTV"
     gene            6205..6417
                     /locus_tag="PK134_03845"
     CDS             6205..6417
                     /locus_tag="PK134_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB38011.1"
                     /translation="MPIVVNLDVMMAKRKVSLNELSERVDLTLSNLSILKTGKAKAIR
                     FSTLEAICKALDCQPGDILEFANTEK"
     gene            complement(6557..6988)
                     /locus_tag="PK134_03850"
     CDS             complement(6557..6988)
                     /locus_tag="PK134_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRB38012.1"
                     /translation="MLSLSCKAGIKAVIFLGSRFESGDKSGIKEISKHINENEHTVGK
                     ILQKLVKNNIIKSVKGPNGGFYITQKQKSQPIINIVEAIDGKDVFKQCGLGLTKCSES
                     RPCPFHNDFKPVRELFKNMCLEKKVSDLYQNVNNGLSYLVG"
     gene            complement(6991..>7298)
                     /locus_tag="PK134_03855"
     CDS             complement(6991..>7298)
                     /locus_tag="PK134_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRB38013.1"
                     /translation="SCLKCTVALAALEHVDDPEIGLNVVDLGLIYEVNFDENEKKITC
                     IMTLTTEFCPMGESITENARNAVQQVFPEYSIDLNLTFDPSWNPEMISEEGQKFLGR"
BASE COUNT         2274 a         1204 c         1319 g         2443 t
ORIGIN      
        1 gaaatatatg atcaggattt tgcagttgac tgctactgct tccatcacca aaattccatg
       61 tccacgactg caaacctgct gccgatgaag actgatctgt taatgttgtt gcagaattgg
      121 cacacgataa attataagta aaatttataa tcggtagtgc atgaatagtt attgggatta
      181 tcgtatcctt ttcgcatcct gcagtgatgg ttagtttaac ctgataggtt cctgatgctg
      241 tatatgcatg ttgtgggttt tgttgtgtgg aggttgttcc atcaccaaaa tcccacaacc
      301 atcctgtaat tgctccggag ggcgaggttg attgatctgt aaaagaaata ctatcattag
      361 gattaaagca tgtattgcct gtaaagttaa actgtgattt tggaccggtg ataacaaata
      421 cactatcata agcaatggat ttatatccgt ttgaatcaga aacagtaagt gataatttat
      481 tccatcctgt atttaaatag ctgaaagaag gattttgatc ttcactgtat tttgtataac
      541 aactgtcaaa ataaagcatg tcaacaaagt tcagtgttgg aacatcgctc actatgaatc
      601 gggtaattga tgaatcggtc agagattcag cctgatttgg acgaaagtaa aaagcaggtg
      661 atgtataaac ggttggcgta ttggcgaagt tattttcatt aaactgcagc catttcaatt
      721 gtttatttgc tgtgtctgtg aaacaaacaa agtaactttc aaattcctgg gtaattgtaa
      781 cagagttgtt accaattgaa cctgtaccgg agtaaacaga cagagaaggg gcattattaa
      841 atccactact aaatgatagt ttggttagat caccattttt ggaatctgta acaaatccta
      901 caggtggagc gcaagtagtt acaatatcaa tgtcagataa attaatgctt cctgttatgt
      961 tgaagctgta taattctaag ggtaaattcg acattgagtt gccaaatgaa agacaggtaa
     1021 cattcccgtt ccctttactt gctataaatg catagtaaat attgtcagca tgaactattt
     1081 taatatctgc agggtttgct actgagctga atgatgacaa aggtgttaat accnnnnnnn
     1141 nnnnnnnnnn nnggggaaag tcatcaagta taaagtgctg gtaggagagt aggcagctaa
     1201 tccataaaca ttggtgtcac cttcaacaat aaagtcaaaa cagtcaagct tggtaaaatt
     1261 gccgtgtaca attgcatcaa aagggcgatt agataaatgg ttgccgatat taccaactac
     1321 aaagttgttg taaatgctat ccatcgtaat cagataatat ccacttccga cttttagtaa
     1381 gtccatatca gtcatagtat aaagcacata attgttgtat cggaaatatt gatacgatgt
     1441 gaaaccatgt atttcctgag gacaaaaatc ccattgatag gtcagtccgt ttcctgttga
     1501 agtgtttgtt aaaggaagtg gaatgccgct acagacagta tcaggtaatg aaaaagaagc
     1561 tgtagggcat tgcgattgtg catttctgct tagactgaat aacagcaata aagcaaaaaa
     1621 tattttctgc attttaaggt ggtttttaaa acaaagtaaa tgtatatatt tttgacttaa
     1681 aaaatgactg agttaatatt ttgttccaac aatgagcata aattacaaga gatacgtaaa
     1741 atgctgcctt tcggttacat tttacagtcg ctttcagagg caggaattca atccaatatt
     1801 gatgaaaccg gtacaacctt tcttgaaaat gcctttctga aagctaatgc cgtttcttta
     1861 ttgactaaga agaattgttt tgcagatgac agtggtttat gtgttgctgc attgaacaat
     1921 gcaccgggag tttattcggc acgttatgca ggaaatgaag ttaccgatgc tgataacaga
     1981 agaaagttgt tgtcggacct ttcaggaata caacaaagaa atgcatattt tatagcagtt
     2041 atttgtttga tttggaaagg taaagctttt tactttgaag gcagaataga tgggatgata
     2101 acccatatcg aacaaggtaa ttcaggattt ggctacgacc ctgtttttat tcctgatgga
     2161 tataaaatca ccttcgcaga aatgtctcct gacgaaaaaa ataaaatcag tcacaggcaa
     2221 attgccgtaa gtaagatgat agcttttttg agaaattcta aagtttaaat gattgcagtt
     2281 cttccggaat ttcaacattc ttaaaaccaa cctcatttag tttctcttta aactcttttt
     2341 gtacttcata ttcgccatgt accagaaaaa gttttttaac tgctgatgta tcctgacaag
     2401 aaagatattt tatcaactct aaataatcgg catgtgcact aaaggatgaa attacttcaa
     2461 cacttgcttt tacaaggtat tcatttccaa aaattttaat tgttttatct ccacgcatca
     2521 aatgcccgcc aattgattca ggtgtgcaat aaccaacaat aagaatggtg cttcgactat
     2581 cattgatgtt gtttttaata tgatgtttta ttcttccggc ttcaatcata cctgatgcag
     2641 aaatgatgat gcaaggtcct ttcagatcat taatcttttt cgactcttca acttttcgta
     2701 catatttaag attactaaag ccaaagggat catcatcaac atgcaggtag ttcactaaat
     2761 cggaattaaa aaactcactg tgtttgcgca tgatatttgt ggcatcaatt gcaagtgggc
     2821 tgtcaacaaa aacaggtata gaaggcagtt tgttttctgt cttaaaacgg tctaatgtgt
     2881 atattatttc ctgagtccgg ccaaggctga atgccggaat tatcaatttg cctttttttt
     2941 caacacaagt tttattgaca atttgtaata atttctgtcg tgcatctgtt gactggtcgt
     3001 gaagtctgtt tccataagta gattcacata aaatgtagtc tgcctgcgaa aatgatgcag
     3061 gcgcttttag gattaaatca ttaaaacgac ccacatctcc actaaaagta atttgttttg
     3121 tattgccttt ttcggtaatg aataaatgca cactgacact acctaaaata tgtcctgcat
     3181 ctgtaaattc gaatgaaatt tcatcgtcat aatgaaagcg tttgttgact gccacagggg
     3241 caaagagttt catagctttt tctacatctt caatttcata aataggctca agtgcttttt
     3301 gtccatctct ttttcgtctt ctgttgatat attcgatatc accactttga atataggcac
     3361 tatcggcaag cattacttcg caaaggtcaa tagtagcttg cgttgtaaag attgttttgt
     3421 taaatccctg cttgactaag tttggaatat ttcctgaatg atcaatgtgt gcatgtgaca
     3481 gaagcatcag gtcaatttgc tgaggatcaa agctaaaatt acgattcagg cgatcattat
     3541 ctttacctct gttctgaaaa aaaccacaat cgagtagtat ttttttccct gattcagtct
     3601 ctatcaggtg tttactaccg gtaacatttc gggcagcacc ataaaaagtt atattcataa
     3661 ggattcaaaa ataatatttt ttttcaataa ctttttatca acattacttc agttttagct
     3721 cagtgttttt agttttgcgg cataatcctt ttaaataaac atcggtttat gtcatttatt
     3781 caatatttta tccctcagga taaaaagttt tttccactat ttgaccaggc agcattaaac
     3841 ctgcaggaag ccggtaaaac tatgtgtcag ttggttactt ctaccgatgc cgatcaacgc
     3901 aaacaactaa ttcgtgaaat agaaagactt gaacatcgtg gcgatgaaat tacacatgag
     3961 atttttaaag agttgagccg taattttatt acacctttcg acagagaaga cattcacagg
     4021 ctagtgagtt ctattgatga tatacttgat tacatacatg gatcatcaaa aagaattgac
     4081 ttatataaag taaaagaatt ttcatctgat atggtgaaac tttcagagtt acttcaaaca
     4141 caaacagaag aactgagacg tgttatctat gagttgaaaa ataagaagaa tatgcgtaac
     4201 atttctgaat cattggttct gattaactca atagaaaatc atgctgatga tatttttgat
     4261 aatgcagtag caagattatt tgaaacggaa accaatgctg ttgaaattat aaaaacaaaa
     4321 gaaattttat cggcattgga aacagcaacc gatatgtgcg aagatgctgc caatgtcata
     4381 gattctatca tagttaaaat ggcctgatga tatgactcat gcacttattg caataatcat
     4441 ccttgcgtta cttttcgatt ttattaatgg ttttcatgat gcagccaact ctattgcaac
     4501 agttgtatca acaaaagtac taagcccatt gacagcagtt atctgggcag catcatttaa
     4561 ttttcttgca tactggattt ttaaacttaa ggtagctgat accgttgcca agactgttac
     4621 acctgatagt gttacgttga tggttgtcgg agcaggtctt gttgcagcaa tcatctggaa
     4681 tctactgacc tggtggtggg gaattccaag cagttcatca catacactta ttggtggttt
     4741 tgcaggagca ggtattgcta atgccggaag ttttagtgca ttgcaagtgg ataaggtaat
     4801 gccaacagtt tattttatta ttctggctcc tattattgga atggtaatgt cttggattat
     4861 atcagtgatt acacttcatt tatgcaggaa atcaaatgta tcacaggtgg ataggacatt
     4921 tagaaaatta cagttattgt catctgctgc ttatagtttg ggtcatggcg gcaacgatgc
     4981 acaaaaagta attggaattg ttggtgcggc catgctttcg catggaatga ttagcgatat
     5041 tgaccatatt cctgagtggg ttccactttc ttgttattca gctattgcac ttggaactat
     5101 gtttggcgga tggcgtattg taaaaaccat gggacagcgt gtcacaaagc tcactccttt
     5161 tgaaggattc agtgctgaag cagcaggggc atttacttta ttttttacgc aacattttaa
     5221 aatacctgta agtacaacac ataccattac aggttctatt attggtgttg gactcactaa
     5281 gagaatttca gctgttcgtt ggggagtaac acttcaactt atctgggcat ggattctaac
     5341 tattcctgtt tccggtgctt tggctgctat catttttatg atagcacgta aagttttcag
     5401 ttaatagata cactcttata agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 natttttttg agtaactcaa gtagtctttc tgaatacttt gtatttgact gtagctgtaa
     5521 tcaaactata atggattgac agaataagcg ttgaatttta tgattttggt tttcaattag
     5581 ttaaactaat attattgaaa aaggaaatat atttattgat aaacgataaa tatatattat
     5641 ttttgcctaa gtaaatcaca attaaatatg gcgaacacta aatttatctt tcaggcattg
     5701 catgtcattg cctggatcat cttcgttggt ctttgtatag aagcaggcgg cattattgta
     5761 aattttattt tcagtatcta caatcccgaa tttgtaagta aactttatca aaagctcgac
     5821 ctaagtgaat tgtacaacag caacaaatgg atcttttaca gtatgtatag tttcatcatt
     5881 gttattgcat tgttgaaagt gtttttattt tacacggtaa tcatgttact gcttaaactt
     5941 gatttgtcca aaccgttcag cagttttgta tcaactcaaa tttcacaaat cagttattat
     6001 acgctttcaa ttgggataat atgtttaata gcagctcata caactaaaaa tttagagcat
     6061 catggttatg tagttgaacg tttatttcaa ttttggaatg atggtcaggc atttattctg
     6121 atgtctgcag tagtttatgt aattgctact atttttaaga agggggttga acttcagaat
     6181 gaaaacgatt taactgtata agctatgccg atagttgtaa atttagatgt tatgatggcg
     6241 aagcgaaaag tttcgctcaa cgagctttct gaacgtgttg acttaacttt atccaatctg
     6301 tcaattttaa aaaccggaaa agccaaggct attcgtttca gtactttaga agcaatttgt
     6361 aaagcattag attgccagcc cggagatatt cttgaatttg caaacactga aaaataaatt
     6421 gatacattac gaatagaggt tgcttatcgt aaatcaactt agcaatatcg tattattaaa
     6481 ttttattctg ttaatttgaa agcagtcatt agtacttaaa actacgtaaa tgcctcctaa
     6541 gttataattt tattttctaa cctactaagt aagaaagccc gttattgaca ttctgataaa
     6601 ggtcacttac ttttttttcg agacacatat ttttaaacaa ctcccgaacc ggtttaaaat
     6661 cattgtgaaa agggcagggg cgcgactctg aacattttgt taatcccaaa ccacattgtt
     6721 taaatacatc cttgccatca atagcttcaa caatatttat tataggttgg cttttttgtt
     6781 tttgtgttat ataaaaacct ccatttggtc ctttaacact tttaatgatg ttgttcttaa
     6841 ctaacttctg taatattttt ccaacggtat gttcgttttc attaatgtgt tttgaaattt
     6901 ctttaatgcc ggacttatca ccagattcaa atctggatcc aagaaaaata acagctttaa
     6961 ttcctgcttt acaacttaaa ctcaacataa ttatcgtcct aaaaattttt gaccttcttc
     7021 cgaaatcatt tcaggattcc atgatggatc aaaagtcagg ttcaaatcta ttgaatattc
     7081 cggaaacacc tgttgtactg catttcttgc attttcagta atggattcgc ccatagggca
     7141 aaactcagta gtaagagtca taatacatgt aatctttttt tcgttctcat cgaaattaac
     7201 ttcgtaaatc aatcctaagt caacaacatt aagtcctatt tcaggatcat caacatgttc
     7261 aagtgcagca agtgcaacag tacattttaa acatgaat
//
LOCUS       DAORQT010000069         7194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1590_length_7194_cov_8.575571, whole genome shotgun sequence.
ACCESSION   DAORQT010000069 DAORQT010000000
VERSION     DAORQT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_7194_cov_8.575571"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK134_03860"
     CDS             complement(<1..336)
                     /locus_tag="PK134_03860"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796865.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRB38014.1"
                     /translation="MDLDQYISLSLQEDVGPGDYSALSCIPADAQSKANLIIKDKGIL
                     AGIELAEKIFKTVDASLQINIFKKDGQHVDKSEIAFTVEGSVLSILKTERLVLNCMQR
                     MSGIASTTYL"
     gene            complement(473..862)
                     /locus_tag="PK134_03865"
     CDS             complement(473..862)
                     /locus_tag="PK134_03865"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRB38015.1"
                     /translation="MKKTIKLLLSVMLIVTVVKAADVFDDIGTAIRSGDARQIARFFN
                     NNVDLTIFNQEEVYSKAQAEMVLKDFFSKNAPKSFTIIHKGVSKEGARYAIGTLTTTQ
                     GQNIRTYFFVKESGGNSSIQELRFEKE"
     gene            1067..1534
                     /locus_tag="PK134_03870"
     CDS             1067..1534
                     /locus_tag="PK134_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRB38016.1"
                     /translation="MQIKLLMIGKNKDSFADAAIAEYASRVERFNSFEVKYLKEAKNN
                     KNLTAAMDAEAENIFKELSPNDFVILLDEKGKEYTSVQFATKIEQLRIKNFKQLVFIA
                     GSAYGFSEKVYARSNELFALSKMTFTHTHARLIFAEQIYRAFTILNNHPYHHI"
     gene            1609..3315
                     /gene="asnB"
                     /locus_tag="PK134_03875"
     CDS             1609..3315
                     /gene="asnB"
                     /locus_tag="PK134_03875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB38017.1"
                     /translation="MCGIAGILNFQHKPVDLSVLKQMSDTLSHRGPDGEGIWHNEQKN
                     IGLAHRRLAIIDLSDEARQPMHYLGRYTIVYNGEIYNYLELKADLEKKGYQFVSHSDT
                     EVILAAFNHKREKCLEDFDGMFAFAIWDSKLQQLFLARDRFGEKPLRYYMDSERIVFA
                     SEVKALFKAEVPKKVSKEKMDNYLQNNISIYNTETFFDGIKKLPHGHYAFVQNGNIDI
                     REYYRLQYKNLVSMDDHEYAEEFKRLFFLSINRRLRSDVPVATSFSGGLDSSGIVCVM
                     HQLKEKVNYKLFSARFGDDVKDEGKWMKHITDLLHFPQINKQIEPQEILEDIEKIVYH
                     NEIPIGSTSVTAQYLLYKEVKNHHVKVILDGQGADEMLAGYGHYRFHYLNDLLLRFRV
                     AEYINERRAYNDAYQTSLPIGPWQLTKMFLTKTVKPFEDNTRGYQSFKATLYNDMMMS
                     LQLLLNFSDSNSMAHGVEVRLPFLYHKLVDFAFSLPANQIYRNATTKYVYRNAMNGII
                     PDAILNRKDKVGFAPPQERWLPQFKLPENARLRDMGLTYSDNHWRNYISEVFLKVADS
                     RY"
     gene            complement(3316..4074)
                     /locus_tag="PK134_03880"
     CDS             complement(3316..4074)
                     /locus_tag="PK134_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB38018.1"
                     /translation="MPSIFITGATAGFGRAIALKFAQHHWSIIINGRRTDRLRTLEDE
                     IKNLGADVISLPFDVRNEQQVNEAIENLPIKWKQIDALVNNAGLAAGVNLIQDGKTED
                     WNTMIDTNVKGLLYVTKNIIPLMIKQKSGHIINIGSIAGKDAYMKGNVYCATKYAVDA
                     LTKSMRIDLLEHGIKVSAVNPGAAETEFSLVRLKGDVEKANAVYKGFEPLVAEDIAEI
                     VYFVATRPAHVCINDVVVTPTAQANTAHLLRKTN"
     gene            complement(4081..5994)
                     /locus_tag="PK134_03885"
     CDS             complement(4081..5994)
                     /locus_tag="PK134_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRB38019.1"
                     /translation="MNLFRKKTVDEIMKRAGDDASQHGSLAKHLGVRDLTALGIAAII
                     GAGIFSTIGNASAQGGPGVILLFLFTAIACGFTAFAYAEFASLVPVSGSAYTYSYVAF
                     GEFIAWIIGWALIMEYGIGNITVAISWSDYLTGLLDSMGIHLPLWMTMDYLTAAKGFS
                     KAGALMLSGRTLESLDAGLRDAYLAWQGAPTIGPLHIVADLPALLIILIITWIVYRGI
                     KESKNAGNAMVIIKLSVVLLVIAVGLFYINTDNWYPFLPNGIPGVLKGVSAVFFAYIG
                     FDAISTTAEECKNPQRDLPRGMMWAIIICTILYIAIALVLTGMVNYSTLAVGDPLAYV
                     FSVLNLKWMSAIIAVSAIVAMASVLLVFQLGQPRIWMSMSRDGLLPKKFATIHPKYKT
                     PSFASIVTGFVVAVPALFMNLTMVTDLCSIGTLFAFVLVCAGVLKMQITPNAPRGSFN
                     TPYVNAKYIFPLMLILTAALSWSYNKDGVSNFLKNTPQPYSAENLIPSLGADRIKDLV
                     NTVSLKDSAAFAKADNDLANYFSQITTDNYLQKLTDYGLSHKEIYESGLGLFKHKIPT
                     WIFIISCLYLCVLTWKHNLSLIPLLGLVCCLYMMSEMGISNWIGFSIWLAVGLIIYFG
                     YSYKNSKLNIIKK"
     gene            6173..>6872
                     /locus_tag="PK134_03890"
     CDS             6173..>6872
                     /locus_tag="PK134_03890"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRB38020.1"
                     /translation="MKKFFRISIIVIAVLFAAALVAPFLFKDKIITAIKNAANENLNA
                     TFDFKDLDLSFIRSFPSVYLALNDVSIVGKDAFKDDTLISASSIQVKAEFWKLITSGN
                     TQVKYFGLLQPRIHLIALKDGKVNWDITKPEKESPTKSEESSPFHFSLQKYEITNGYL
                     TYNDEALAMNTKIDNLNHTGKGDFTQDNFLLNTLTSIDKLTYTYAGVSYLYQAKVNLK
                     ADLNIESKTSKYTFA"
     assembly_gap    6873..6906
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6927..>7194
                     /locus_tag="PK134_03895"
     CDS             6927..>7194
                     /locus_tag="PK134_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane assembly protein AsmA"
                     /protein_id="HRB38021.1"
                     /translation="MDLKFNSPQTDFKYLMSLVPGAYKENFKDVKSSGKLAFNGFVKG
                     VYSEHTMPGFGINLTVDNGNFKYPSLPASINNVNLKLAVSNPDGE"
BASE COUNT         2328 a         1324 c         1276 g         2232 t
ORIGIN      
        1 taggtatgta gtggaggcaa tgccgctcat acgctgcata cagttaagta ccaaacgttc
       61 tgtcttcagt atagaaagaa cgcttccttc aactgtaaaa gcaatttcac ttttatcaac
      121 gtgttgccca tcttttttaa agatgttgat ttgcaacgaa gcgtcaacag ttttaaatat
      181 tttttctgca agttcaatgc ctgcaagtat tcctttatct ttaataataa gattggcctt
      241 gctttgagca tctgccggaa tgcaacttaa agcactgtaa tcaccgggtc cgacatcttc
      301 ctgtagtgat aatgaaatgt attgatctaa atccatgata attatttctg ctgcaaaatt
      361 cagctttttg attcaaagaa tcatgtagga tacgattttt ttaaaatact aaaaaggaaa
      421 aagccgtaaa caagagtgaa tacggctttc aaaaactatg gggaagatat tcttattctt
      481 tttcgaagcg caattcctga atagaagaat tgccaccact ttcttttaca aagaaataag
      541 ttctgatatt ctgaccttgc gttgtagtga gtgttcctat tgcataacgg gcaccttcct
      601 tcgaaacgcc tttatggatt atggtgaaac tttttggtgc gtttttactg aaaaaatctt
      661 taagcaccat ttctgcctgc gctttactgt aaacttcttc ctgattaaat attgtcaaat
      721 caacattgtt attaaagaaa cgagcaattt gtctggcatc gccagaacgg atggcagtgc
      781 cgatatcatc gaaaacatca gctgccttta caacggtaac tatcagcatg acagaaagaa
      841 gaagcttgat ggtttttttc atccttgata aattatgttt gtgaactgac aaaaatcaag
      901 ccaagagcgc ataggaatga aaaacttatg aatgcatttt agcataaatc ttacagtaat
      961 aattaaacac ttaaatgtaa atccgaaaca atattaaggc ataaaaattt actttcaaaa
     1021 cttaatttca gatttttgta aataatcatt attggataac gtaattatgc agatcaaatt
     1081 gttaatgata ggtaagaata aggatagttt tgccgatgcc gcaatagctg agtatgcgag
     1141 tagggttgag aggtttaaca gttttgaagt caagtatctg aaagaagcca aaaacaacaa
     1201 aaaccttaca gcagcaatgg atgctgaggc tgaaaatatt ttcaaggagt tatcgccaaa
     1261 tgactttgtt attctgctgg acgaaaaagg caaagaatac acatcggtgc agtttgcaac
     1321 aaaaatcgag cagttaagga ttaaaaattt caaacaattg gtttttattg ctggtagtgc
     1381 ttatggtttt tcggaaaaag tttatgcacg aagtaatgaa ctgtttgccc tgtcaaaaat
     1441 gacattcacc catactcatg caagattgat ttttgccgaa cagatttaca gagcatttac
     1501 tatcctgaat aatcatcctt atcatcatat ttaatttcat cggcatacta tttggtaaga
     1561 tggaaaatct aattttgcac tttcaaaaat ttttgatcag aaatctatat gtgcggtata
     1621 gccggcattc tcaattttca gcataaacct gttgatttat cagtattaaa gcaaatgtca
     1681 gacacccttt ctcacagggg gcctgatggt gaaggtattt ggcataacga acagaaaaat
     1741 ataggacttg cacacagacg attggcgatt attgaccttt ctgatgaagc caggcagcca
     1801 atgcattatt taggtcgtta tacaatagtc tataatggag aaatttataa ttaccttgaa
     1861 ctgaaggctg accttgaaaa gaaaggctat cagtttgtgt cgcactccga cactgaggtg
     1921 attcttgcag catttaacca taagcgggaa aagtgccttg aagattttga cggtatgttt
     1981 gcctttgcaa tatgggacag taaattgcag cagttatttt tagcccgtga ccgttttgga
     2041 gaaaaacctt tacgttacta tatggatagt gaacggatag tttttgcttc ggaagtaaaa
     2101 gcattgttta aagccgaagt tccgaagaag gtttctaaag agaagatgga taactacctt
     2161 cagaataata tttcaattta taacactgaa acattttttg atggaataaa gaaactgcca
     2221 catggtcatt atgcttttgt gcagaatggc aatattgata tccgtgagta ttacagattg
     2281 caatacaaga atcttgtatc catggatgac catgaatatg cagaggagtt caaaaggttg
     2341 ttttttttat caataaacag acgattgcga agtgacgttc ctgtggcaac atcattttcc
     2401 ggagggcttg attcctccgg tattgtttgt gtaatgcatc aactcaagga aaaagttaat
     2461 tataaattat tttcagcacg atttggagat gatgtaaaag atgaaggtaa atggatgaag
     2521 catataacgg atttacttca ctttccacag ataaataaac agattgagcc ccaagaaatt
     2581 ttagaagata ttgaaaaaat tgtctatcat aatgagattc ccattggatc cacatcagta
     2641 acagcacaat atctgcttta taaggaagtt aaaaatcatc atgttaaagt tattcttgat
     2701 ggacaaggtg ccgatgaaat gctggcaggg tatgggcatt accgtttcca ttatctaaac
     2761 gatttgttgc ttcgcttcag ggtagcggaa tatattaatg aaaggcgagc atataatgat
     2821 gcatatcaaa cttcactccc aattggccct tggcaactga caaagatgtt tctcacaaaa
     2881 actgtaaagc catttgaaga taacactcgg ggatatcagt catttaaagc tacactttat
     2941 aatgatatga tgatgagtct tcaattgttg ttaaacttct ctgatagtaa ttctatggct
     3001 cacggtgtgg aagtaaggct tccatttttg tatcataagt tggttgactt tgccttttca
     3061 ctacctgcca atcagattta cagaaatgca acaacaaagt atgtttaccg caatgctatg
     3121 aatggaatta ttcctgatgc tattctcaat cgtaaagata aggtagggtt tgcaccacct
     3181 caggaacgat ggcttcctca atttaaactc ccggaaaatg caagacttcg tgacatgggt
     3241 ttaacttatt ctgataacca ctggcgcaat tacatctcag aagtattttt aaaagtagcc
     3301 gacagccgat actgattaat tggtttttcg caataaatgt gctgtatttg cttgcgcagt
     3361 tggtgtaact acaacatcat ttatacaaac atgtgcagga cgggttgcaa caaaatatac
     3421 aatctctgca atatcttccg ccactaatgg ttcaaatcct ttataaacag cattggcttt
     3481 ttcaacatca cctttcaatc taaccaaaga aaattctgtt tctgcagcac ctggatttac
     3541 tgcactgact tttatgccat gttctaataa atcaatacgc atactttttg tcaaagcatc
     3601 tactgcatac tttgtggcgc aataaacatt gcctttcata taagcatctt ttccggcaat
     3661 agaacctatg ttgatgatat gtccgctttt ttgttttatc atcagtggaa tgatattttt
     3721 agtgacataa agcagtcctt tcacattagt atcaatcatt gtgttccagt cttcagtctt
     3781 gccatcctga atcagattta ctcctgctgc aagtcctgca ttattaacta atgcatcaat
     3841 ttgtttccat ttgatgggga gattttcaat agcttcatta acctgttgtt cgtttcgaac
     3901 atcaaaagga agactgatga catctgctcc aagattcttt atttcatctt ctaaagtgcg
     3961 aagtctgtca gttcttctac cattaataat aatgctccaa tggtgttgtg caaattttaa
     4021 tgctatggct cttccaaatc ctgctgttgc accggtaata aaaattgaag gcataataat
     4081 ttattttttt ataatattta acttactgtt tttatagctg taaccgaaat aaataatcag
     4141 ccctacagcc agccaaatcg aaaacccaat ccagtttgat atgcccattt cactcatcat
     4201 gtataggcag caaaccaaac ctaataatgg aatgagcgat agattgtgtt tccaggttaa
     4261 aacacaaaga tacagacatg aaatgataaa tatccatgta ggtattttat gtttaaaaag
     4321 tccaagaccc gattcataaa tttctttatg cgacaatccg tagtccgtta gtttctggag
     4381 ataattatcg gtagttattt gtgaaaaata atttgcaagg tcgttgtcgg ctttagcaaa
     4441 tgctgctgag tctttcaaag aaacagtgtt taccagatct ttaattctgt cagctcctaa
     4501 agaaggaatc agattttcgg cagagtaggg ttgtggagtg ttcttcaaaa aattggaaac
     4561 gccatcttta ttatatgacc acgacaatgc tgcagttaaa atgagcatta acgggaaaat
     4621 atatttagca tttacataag gtgtattaaa actacctcgg ggcgcattag gcgtgatttg
     4681 cattttcaaa actccggcac aaactaacac aaaggcaaat aaggttccaa tgctacaaag
     4741 gtctgtaacc attgtaaggt tcatgaaaag tgctggcact gcaactacaa agccggttac
     4801 aattgatgca aacgatggtg ttttatattt tgggtgaatg gttgcaaatt tcttaggtag
     4861 caaaccatct ctgctcatac tcatccagat gcgtggttga cccaattgaa aaaccaacaa
     4921 tacacttgcc attgcaacaa tagcactcac tgcaataatt gccgacatcc attttaaatt
     4981 caatacactg aaaacataag ccaatgggtc accaacagcc aaagtgctgt agtttaccat
     5041 tcctgtaagc actaatgcta tggcaatata aagtattgta cagattatta ttgcccacat
     5101 cattcctcga ggtaaatcac gttgtggatt tttacattct tctgctgttg tgctgatagc
     5161 atcaaaacct atgtatgcaa aaaatactgc tgacacacct tttaaaacac cgggaatgcc
     5221 attgggtaaa aaaggatacc agttatcggt attaatataa aacaaaccca cagcaatgac
     5281 taaaagcaca acagaaagtt ttatgattac catggcatta cctgcatttt tcgactcctt
     5341 aattcctctg taaacaatcc atgtaatgat gagaataatt aaaagagcag gcaaatcagc
     5401 aacaatatgc aatgggccta ttgttggagc accctgccat gctaaatagg catcacgcaa
     5461 accggcatca agactttcaa gtgttcttcc gctaagcatc agtgcgcctg ctttgctgaa
     5521 cccttttgct gctgtaagat aatccatcgt catccacagt ggcaaatgta tgcccatact
     5581 gtcgagtaac cctgttaaat aatcgctcca tgaaatagca acggtaatgt ttccaatgcc
     5641 atactccata atcaatgccc atccgattat ccatgcaata aattcaccaa aggctacata
     5701 gctgtaagta tatgcactcc cactaacagg aactaatgat gcaaactctg cataagcaaa
     5761 agcggtgaaa ccacaggcaa tggcagtgaa aagaaacaat aaaatcacac cgggtccacc
     5821 ttgtgcactt gcattaccaa tagtggaaaa aattcctgca ccaattatgg cagcaatgcc
     5881 caaagcagtg aggtcgcgca cacctaaatg ctttgctaac gagccatgct gtgaggcatc
     5941 atcaccggca cgtttcataa tttcatcaac tgttttcttt ctgaataaat tcatgaacag
     6001 acattattat ccggtcaaaa ataaaataat ttcatccgca aaggtctttt tcttgtttaa
     6061 tgttgattaa ttcattatat attctcatgt caattggctg ctattaaata gttttgcagt
     6121 catcaataat tcaatcattt atccgttaac acaaaggtaa aatcacttat ttatgaaaaa
     6181 gttttttcgc atttctataa ttgttattgc agttcttttt gcagcagctc ttgttgcacc
     6241 ttttttgttt aaagataaaa ttattacagc cattaaaaat gcagctaacg aaaacctgaa
     6301 tgcaacattc gattttaaag acctggattt aagctttatc cgtagtttcc cctctgtgta
     6361 tttagcatta aacgatgttt ccattgttgg gaaagatgca ttcaaagacg atacacttat
     6421 ttcagcttcg tccatacagg ttaaagctga gttttggaaa ctcattactt caggaaacac
     6481 acaggttaaa tattttggat tattgcagcc acgcattcat cttattgctc ttaaagacgg
     6541 aaaggtaaac tgggacatca ccaagcctga aaaggaatca ccaacaaagt cggaagagtc
     6601 ttcaccattt catttttcat tacagaaata tgaaatcacg aacggttatc ttacctacaa
     6661 cgatgaggct ttggcaatga ataccaaaat agataatctg aatcatacag gcaagggtga
     6721 ctttacacag gataattttt tactgaatac cttaacttca attgataaac ttacttatac
     6781 ttatgcaggt gtaagttatt tgtatcaagc taaagttaac ctcaaagcag atttaaacat
     6841 tgagtctaag acatcgaaat acacttttgc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnntgcc cacagacgat attagtatgg atttgaaatt taattcacca caaacagatt
     6961 ttaaatatct gatgtcattg gttccgggtg catataaaga aaattttaaa gatgtaaagt
     7021 cttctggaaa gctggccttc aatggttttg taaaaggtgt ttattcagaa cataccatgc
     7081 ccggatttgg tattaattta actgttgaca atggaaactt taaatatcct tcattacctg
     7141 catcaatcaa caatgtaaat cttaaattag ctgtttccaa tcccgatggc gaac
//
LOCUS       DAORQT010000070         7181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1594_length_7181_cov_9.293713, whole genome shotgun sequence.
ACCESSION   DAORQT010000070 DAORQT010000000
VERSION     DAORQT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_7181_cov_9.293713"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK134_03900"
     CDS             <1..263
                     /locus_tag="PK134_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRB38022.1"
                     /translation="KIPIDEKTYNLAREFHLDPTVCAMNGGEDYELLFTINQKDFEKI
                     NSEADISIIGHITAKEEGYYMLDKSDRKVALKAQGWDALKSN"
     gene            281..1060
                     /locus_tag="PK134_03905"
     CDS             281..1060
                     /locus_tag="PK134_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRB38023.1"
                     /translation="MSNKNYRIICWNVNGIRAIWKKGFPEWLSKEMPDVLCLQETKAQ
                     PEQLDDEIKDFNGYKSHFFSAQKKGYSGVAIYTKREPLKVRYGLDDDAFDIEGRCIEM
                     EFEDFVLYTAYFPNGGRGPERVKYKLDFYESLFQRAENMRKKNKNIVICGDYNTAHQE
                     IDLARPKENSKTTGFLPIEREWIDKIIGMGYVDIFREFNQESGQYTYWDQITRARDRN
                     VGWRIDYYMISTELRKYAKSAPIHMNVMGSDHCPIELNLSF"
     gene            complement(1089..2072)
                     /locus_tag="PK134_03910"
     CDS             complement(1089..2072)
                     /locus_tag="PK134_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRB38024.1"
                     /translation="MQHDPYVILGISPSATPEEIKRAYRKKAFELHPDRNSDQHAEQL
                     FRELNEAYALLTDADKRRNYDKQYQNYSRSNTDQFDHYFEMHVSSNNVKVCEEFELIF
                     TYTGEGRFMQKPDLRDFYITGKPFVSFRDVYRSGKTARETTIRYVLAAKRVGSFTINR
                     AAIKLFGKKFITATQYIQVISGECFFSPDKPADGRPLQVSLWYNAEKGGTNRKYLVNT
                     KHTIYIPRSNYAQVYHTIGRVLKLIFAFWGFFVAVKISRSGIIGFAIGSAYGAIMTYL
                     LYYFVKVKPVFMSERKYSLVISYLQNGYQPYPPHARNLISRALMFINKLFL"
     gene            2165..4021
                     /locus_tag="PK134_03915"
     CDS             2165..4021
                     /locus_tag="PK134_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB38025.1"
                     /translation="MKNYFKILRYVKPYWGYALLNASCNILSVLFSFFSLTLIAPFLD
                     LLFLKDDNFYLEKMAAGKPEFHLSTKAIVDSFYYYLTEMITDPNRGKQYALMFICIAV
                     AVMFFLKNLFRYLGQFFISPIRNGVVKDLRADVHKKMMRLPLSWFSNERKGDIITRVT
                     SDVHEVEWSVMSSLEVVFRDPFNIFLFLGMMIFISAKLTVFVFILLPIVGFLIGRIGK
                     SLKRTSARSKEKLGLLISIIEETLSGLRIIKAFNAEKFMAEKFAKVNERYNTIAIRAL
                     RKTDLSSPLSEFLGTVTMMIVMYFGGMLVLGKDASLSASAFITYIALFSQIIPPAKSF
                     TQAFYNIQKGLASADRIFAVLEAEESIKDQQSAETIKSFNYKIEYQNISFSYRKGDEG
                     YVLKNVSFTIEKGKTVALVGQSGSGKTTLADLLPRYYDVDGGTLLIDGTDIRKLQLHS
                     LRELLGVVSQEPILFNDTIYNNIAFGMNHATEKMVEEAASVANAHEFITQMPEGYQTN
                     IGDRGSKLSGGQRQRISIARAVLKNPPILILDEATSALDTESEKLVQDALTSLMKNRT
                     VLVIAHRLSTIQHADEIIVLQKGEIVQRGTHVQLANVEGVYKKLYELQGLSS"
     gene            complement(4031..4792)
                     /locus_tag="PK134_03920"
     CDS             complement(4031..4792)
                     /locus_tag="PK134_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRB38026.1"
                     /translation="MKIVIFEDEEPASRRLIKLLNEINANVEIVSVIDSVRSGKEFMS
                     QNKNFDLIISDIRLADGLSFEIFKSYPVKLPVIFSTAYDEYAIEAFRNNGIDYLLKPV
                     KREELKSAIQKYENWFSDSTSGVNYDALLQTLLQEKKGFQKRIVIRFADTIKTVEIED
                     AAYFFTENKINNLCTFGGESFIVDHNLDELELMLDPSVFFRINRQFIVNFKAIQKMNV
                     VSKSRVKLTLHPTTIQDTIVSTERSGVFKQWLAGK"
     gene            complement(4792..>7181)
                     /locus_tag="PK134_03925"
     CDS             complement(4792..>7181)
                     /locus_tag="PK134_03925"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRB38027.1"
                     /translation="SAPDKSGNMWIGTDQGLSLCTSKNGKKVLQKLQVADGLCDNIVK
                     KIVIEGSKMFLATQENGLCIYDFEQNGFLKQKAETVWNYGSIDDIILNKTTNEIWVAT
                     ESGGLLYFRNPEEKTYSLKQQSAKLFSSVYSVAIDHQNNVWVATDRGLFRSHGQWLTF
                     LEQYAGADINNVHEVAFLDSNQMVVSIKNKIMLISFNTSTIKEWTILPSEKNIDVSGL
                     YIDKEKYIWIGTLGNGVFRINPLKSEIRKIEIPFDYSSILYINGNEKNIWLGTFGGAA
                     LIEVEQLNTDNLKYKVRIFSHESPLGNYYVYCVYRDVKGRFWFGTDQNGIVLLDDNKF
                     VAPFSGKFNGRTVYSITGDNNDAIWFSVADEGIVCLNGDSFKLYNRSNGIRDLNITSI
                     AVLDDNSIAIVNKRGIDILNHKSGSVMYLGTENNLDNLNSDINSVAKDSIGRIWFGTV
                     KGMVVLDPKLLKQNTVPELSKLNMIQPIFKNRSGITPVFRHNENTVTFEYAATWFTNP
                     GRILYQYKLDGLNADWIATKDIKQVFPNLPPGKYVFRIRTSIDPQFTFNNESSFSFEI
                     KKPFWQEDWFYFSSLLLAILLVYLFIKVRDARMKKLQQLKNDSIRFQFETLRNQVNPH
                     FLFNSFNTLIDIIEHDKEHAVDYVENLSAFFRNIVTYKDKETIKLSEEIKIAKAFYFI
                     QQKRFGSNLQLLIDEHSLYDEKYVVPMVLQLLLENAIKHNEISKEKNLEVHVAFTNDR
                     IIISNNTNPKVSKDESTGTGLRNIIARYRLLTDKTVQIESSQDYFVVSLPLINHQ"
BASE COUNT         2287 a         1281 c         1226 g         2387 t
ORIGIN      
        1 ctaaaattcc tattgatgaa aagacttata atctggcacg ggagtttcat ttagacccaa
       61 cagtgtgtgc catgaacggt ggtgaagact acgaactgtt atttactatc aaccaaaaag
      121 attttgaaaa aattaacagc gaagctgaca tatctattat cggtcacatc actgctaagg
      181 aagaaggtta ttacatgctc gataaatctg accgtaaagt ggcattgaaa gcacaaggct
      241 gggatgcatt aaaatcaaat taaaccttta tcactaaaaa atgagtaaca aaaattatcg
      301 cattatttgt tggaatgtaa acggcataag agctatctgg aaaaagggat ttccggaatg
      361 gttgtcaaaa gaaatgcctg atgtattatg tcttcaggaa accaaggcac aacctgagca
      421 attggatgat gaaataaaag atttcaatgg ctacaaaagt catttttttt ctgcacagaa
      481 aaaaggatac agcggtgttg ccatttacac caaacgtgag ccattgaaag taaggtatgg
      541 attagatgat gatgcttttg atatagaagg ccgttgtatt gaaatggagt ttgaagattt
      601 tgtgttatac actgcatatt tcccaaatgg tggtcgtggt cctgaacgtg tcaagtataa
      661 actggatttt tacgaatcac tttttcagag agctgaaaac atgagaaaga aaaataaaaa
      721 cattgtcatt tgcggagact ataatactgc acatcaggaa atagatttgg cacggccgaa
      781 agaaaattca aagacaacag gatttttgcc tattgaacgc gagtggattg acaagattat
      841 cggcatgggg tatgttgaca ttttcaggga gtttaatcag gaatccgggc aatacaccta
      901 ctgggatcag attacacgtg cccgcgacag aaatgtaggc tggcgcatag attattatat
      961 gatttcaact gaactgcgca aatacgcaaa gagtgctccc atacacatga atgtaatggg
     1021 cagtgatcat tgtccaatag aactgaattt gtcattctaa atctccgttc aaaatttttt
     1081 atctcacttt acaaaaacag tttgttgata aacatcaatg cacggctaat caagttacgc
     1141 gcatgaggag gataaggctg gtagccattt tgaagatagg agattaccaa tgaatatttt
     1201 ctttctgaca taaaaacggg cttcaccttt acgaagtagt aaagcagata ggtcataatt
     1261 gctccataag cagagccaat ggcgaatcct ataataccac ttctgctaat cttaactgca
     1321 acaaaaaatc cccaaaacgc aaaaattaat ttaagcactc tgccaatagt atggtaaacc
     1381 tgtgcataat tacttctggg aatataaatg gtgtgtttgg tgttgactaa atattttctg
     1441 tttgttcctc ctttctcggc attataccat agtgaaacct gcaaaggtct tccatcagca
     1501 ggcttatcag gagaaaaaaa acattcaccg gaaataacct gaatatactg agttgcagta
     1561 ataaactttt taccaaataa cttaatagca gcacgattta ttgtaaaact acctactctt
     1621 ttcgctgcaa gcacatagcg aatggttgtt tcccttgcag ttttacccga acgatataca
     1681 tcgcgaaatg atacaaatgg tttaccggtt atataaaaat cacgcaaatc tggtttttgc
     1741 ataaatcgtc cttcaccggt ataggtaaaa atcaactcaa actcttcgca cacttttaca
     1801 ttattactgc tgacatgcat ttcgaaataa tggtcaaact gatcggtatt gctgcgtgaa
     1861 tagttttgat attgcttatc ataatttctt cttttatcag catcagtcag taaggcataa
     1921 gcctcattca actccctgaa tagttgttca gcatgttggt cgctgttacg gtccggatgt
     1981 agttcgaaag cttttttacg ataagcccgc ttaatctctt cgggagtggc cgaaggtgat
     2041 atacctaaaa ttacatacgg atcatgttgc atttagcgtc tgtgggtaac aattaaacaa
     2101 tattgaacat ttttgtgtta gtttgcaact gttttcagga ttttatttac aagaactatt
     2161 ttttttgaaa aattacttta aaatacttcg ttacgtaaaa ccttattggg gctatgcatt
     2221 attgaatgca tcgtgcaata tcttatcagt tctgttttca tttttttcat taacattaat
     2281 tgctcctttt cttgatttat tgtttctgaa agatgataac ttttatttag agaaaatggc
     2341 tgccggcaag ccggagtttc atttgtcaac aaaggctatt gttgatagtt tctattatta
     2401 tcttactgaa atgatcactg acccaaacag gggtaagcaa tatgcattaa tgtttatttg
     2461 tattgcagtg gcagtgatgt tttttcttaa aaaccttttt cgctacttag ggcaattttt
     2521 tatttctccc atcagaaatg gtgtagttaa agatttacgt gctgacgtac ataaaaaaat
     2581 gatgcggtta cctttatcat ggtttagcaa tgaacgcaaa ggcgatatta ttacaagggt
     2641 gacttctgat gtgcatgaag tggaatggag tgtgatgagc tctttggaag ttgtttttcg
     2701 cgaccctttc aatatatttt tgtttttagg catgatgatt tttataagtg ccaagctcac
     2761 tgtttttgtt tttattctgc ttcctatagt aggatttctg attggacgta ttggcaaaag
     2821 tcttaaacgt acatcagcca gaagcaaaga aaaacttggg ctgctgattt caattataga
     2881 agaaacacta tcaggcctgc gtataattaa agcatttaat gcagaaaaat ttatggcaga
     2941 aaagtttgca aaagttaatg agcgttataa taccattgcc atacgtgcac ttcgcaaaac
     3001 tgatttatca tcacctctga gtgaattttt aggcactgtc accatgatga ttgtaatgta
     3061 ttttggtggt atgcttgtgt tgggaaaaga tgcatcactt tcggcttcgg catttatcac
     3121 ttatatagca ctattttcgc agattatacc acctgcaaaa tctttcactc aggcatttta
     3181 caatattcaa aaggggcttg cctctgccga cagaatattt gctgttttgg aagcagaaga
     3241 aagtattaag gatcaacagt cggcagaaac aattaaatca ttcaactata aaattgaata
     3301 tcaaaatatt tctttttctt acagaaaagg tgatgaaggt tatgttttaa aaaatgtaag
     3361 tttcactatt gaaaaaggaa aaactgttgc attggtaggc caatcaggat caggcaaaac
     3421 tacattagca gatttattgc ctcgctatta tgatgttgat ggcggtacac tgctgattga
     3481 cggcacagat attaggaaat tacaattgca tagcctcaga gagttgctgg gtgttgtctc
     3541 acaggaacca attcttttta atgacaccat ttacaacaac attgctttcg gaatgaacca
     3601 tgcaacagaa aaaatggttg aagaggctgc aagtgttgcc aacgcacatg agtttattac
     3661 tcaaatgccg gaaggctatc aaaccaacat tggcgacaga ggtagtaaac tctccggagg
     3721 gcagcgtcaa cgtattagca ttgcaagggc agttctaaaa aatccaccga tattgattct
     3781 tgacgaagct acatctgctt tagatactga aagcgaaaaa ttagttcagg acgcactgac
     3841 aagtctgatg aaaaaccgga ctgtattagt cattgcacac agactaagta ccattcaaca
     3901 tgccgatgaa attattgttt tacagaaagg agaaattgtt caacgcggta ctcatgttca
     3961 gcttgcaaat gttgaaggtg tttacaaaaa gctttacgaa ctacaaggtt taagttcctg
     4021 atttgaactt ttacttacct gctaaccact gtttaaaaac gccactacgc tctgtactaa
     4081 ctattgtatc ctgaatggtt gttgggtgaa gtgtaagctt aactcttgat ttagaaacaa
     4141 cattcatttt ttgtattgct ttaaaattaa cgataaactg cctgttgatt ctaaaaaaga
     4201 cagaggggtc aagcattagc tctaattcat ctaagttatg atcaacaata aatgattctc
     4261 caccaaaggt acaaaggtta tttattttat tctcagtaaa aaaataagca gcatcttcaa
     4321 tttctactgt cttaatagtg tctgcaaaac gaattacaat tcgcttctga aagccttttt
     4381 tttcttgcaa cagtgtctgc aacaaagcat cataattaac tccagatgtt gagtcagaaa
     4441 accagttctc atatttttgt attgcagatt ttagttcttc tctttttacc ggcttcaata
     4501 aataatcaat tccgttgtta cgaaaggctt ctattgcata ttcatcataa gccgtactga
     4561 atattaccgg aagctttaca ggatagctct taaaaatttc aaaacttaat ccatctgcaa
     4621 gtcttatgtc tgaaataata agatcgaaat ttttattctg actcataaat tcttttccgg
     4681 aacgtacaga atcaatgact gaaacaatct ccacattagc attaatctcg ttcaggagtt
     4741 taatcagtct gcgtgatgcc ggttcttcgt cttcaaaaat tactatcttc attactgatg
     4801 atttattaat ggcaatgata caacaaaata atcttgtgat gattcaatct gtaccgtttt
     4861 atcagtgagt aaacgatagc gtgcaataat atttcgaagt ccggttcctg ttgattcatc
     4921 tttactcact tttgggtttg tgttattgct aattattatt ctatcgttag taaatgcaac
     4981 atgcacctcc agattttttt ctttagaaat ttcattatgt ttaattgcat tctcgagtaa
     5041 taattgtaat accataggaa caacatattt ttcatcatat aagctatgtt cgtcaattaa
     5101 aagttgcaga tttgaaccaa atcttttttg ttgaataaaa taaaatgcct ttgcaatttt
     5161 aatttcttct gatagtttta ttgtctcttt atccttgtag gtgacaatgt ttctgaaaaa
     5221 tgcagaaaga ttttctacat aatcaactgc atgttcttta tcatgctcaa tgatatctat
     5281 gagtgtatta aaactgttaa acagaaaatg tgggtttacc tgattgcgca atgtctcaaa
     5341 ctgaaatctg atagaatcgt tttttagttg ctgcagcttt ttcattcttg catccctgac
     5401 ttttataaag aggtaaacta ataaaatcgc tagcagcaga cttgaaaaat aaaaccaatc
     5461 ttcttgccaa aatggttttt ttatctcaaa cgaaaaacta ctttcattgt tgaatgtaaa
     5521 ctgtggatct attgaagttc ttattctgaa aacatattta cccggtggca aattaggaaa
     5581 tacttgttta atatctttag ttgcaatcca gtctgcgttc aagccatcta atttatactg
     5641 ataaagaatt cttccgggat tggtaaacca agttgccgca tattcaaagg ttactgtgtt
     5701 ttcattgtgc ctgaacactg gcgtaattcc ggaacgattt ttaaatatcg gttgtatcat
     5761 gtttaattta ctcaactccg gaacagtatt ttgtttcagc aactttggat ctaatactac
     5821 catacccttg actgtgccaa accagattct cccaatacta tctttagcta cagaattaat
     5881 gtcagaattc aggttatcga gattgttttc tgttccaaga tacataactg aacctgactt
     5941 atgattcaat atatcaatgc ctcttttatt aactattgca atgctgttat catcaagcac
     6001 tgcaatactg gttatgttca aatcgcggat tccattggag cggttatata atttaaaact
     6061 gtctccatta aggcaaacaa ttccttcatc agctacgcta aaccatattg catcattatt
     6121 atcacctgtt attgaatata ctgttctgcc attaaatttc cctgaaaaag gagcaacaaa
     6181 tttattgtca tctaacagca ctattccatt ctgatcggta ccaaaccaaa accgcccttt
     6241 tacatcacga taaacacaat aaacataata atttcctagt ggtgattcat gagaaaaaat
     6301 tctgacttta tatttaagat tatcagtgtt taattgttct acttcaatta aagcagcacc
     6361 cccaaatgtt ccaagccaga tatttttttc attaccgttt atatataaaa tactgctata
     6421 atcaaatgga atttctattt tcctgatttc actctttaaa ggatttatcc tgaaaacacc
     6481 gttacctaaa gtacctatcc atatatattt ctctttgtca atatataaac cggaaacatc
     6541 aatatttttt tctgaaggta gaattgtcca ttcttttata gtagatgtat taaatgatat
     6601 gagcattatt ttatttttaa tactcacaac catttgattg ctatctagga atgcaacctc
     6661 atgtacatta ttaatatcag caccagcgta ttgttctaaa aatgtcagcc attgtccatg
     6721 tgatcgaaat aaaccacggt ctgttgcaac ccaaacatta ttctgatgat ctattgctac
     6781 tgaatataca gagctaaata attttgctga ctgttgtttt aaagaatagg tcttctcctc
     6841 aggattccga aaatataaaa gtccaccgga ttctgttgca acccaaattt cattagttgt
     6901 cttatttaga ataatatcat ctattgatcc ataattccaa accgtctccg ccttttgttt
     6961 cagaaaacca ttctgctcaa aatcataaat acataatccg ttttcttgtg ttgcaagaaa
     7021 catttttgaa ccttctataa ctattttctt aactatatta tcacataatc catccgcaac
     7081 ctgaagcttt tgaagaactt tttttccatt ttttgatgta caaagcgaca atccctgatc
     7141 tgttccaatc cacatattac ctgatttatc cggggcagaa g
//
LOCUS       DAORQT010000071         7112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1615_length_7112_cov_8.127958, whole genome shotgun sequence.
ACCESSION   DAORQT010000071 DAORQT010000000
VERSION     DAORQT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_7112_cov_8.127958"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK134_03930"
     CDS             <1..430
                     /locus_tag="PK134_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRB38028.1"
                     /translation="DCSVWFNAVVRGDVHSITIGNKVNIQDGAIIHCTYQKAPTVIGN
                     NVSIGHNAIVHGCTLEDNVLIGMGAIVMDHAIIKTNAIIAAGSVVLENTVVESGCIYA
                     GVPAKKVKEVSPEHFKDLNERIANNYVMYSGWFKKDTDEK"
     gene            420..1805
                     /locus_tag="PK134_03935"
     CDS             420..1805
                     /locus_tag="PK134_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38029.1"
                     /translation="MKNRVILFWLFLNICFLFFSCATPEKKESVQQVDSSTVVQTHFT
                     KIKLEPGKLLSNVKSESNSNVSFSVYAPSEYSDTTLLPIIYFFDPHADGNLPLEKYKS
                     IANTFNLILVGCNSIENGMPFEQSQQIANILFSDCQNRLPINTQQQIIAGFSGGSKVA
                     CNLAMNQHFFTGLIACSGALFENGKFDKNMSVVSVAGINDFNYHEMVALEKSVDVTHH
                     AFIETDSTHQWPGTSDMYDATLFTLMQMVKQHKLQMQSSVVENFYHTWLKKINDSANK
                     SSPGKQFDQLQFGIMCFDGLTDVTSLKSKLKEIENSDNLRKWRMRSQNMQYQEKEMQK
                     MLRDAFSNQNLAWWQKEITDLKTAATNSNDKMMAAMSNRLLGFIGIACYSFAKQLVNE
                     NAQESDKVLQIYQTVEPNNSEAWFLSAVYHANNKQPEKAMTDFNKAVQNGFNQTSRIN
                     EYPALQQIVAP"
     gene            1921..2799
                     /locus_tag="PK134_03940"
     CDS             1921..2799
                     /locus_tag="PK134_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38030.1"
                     /translation="MKRLMLLATGMFLCLTAVEAQPYKQAGGENNLQLLFAPFGSTPL
                     SLNEGITYRRFLKDGKLAARLSISISSSKSTDVIMQADDTLAFPTTTTVFHAEGGGGY
                     IQLTTGMNPQADLVSKTSGFSFRPGIEKHFEGTDRLSPYIGAEIVFSSSKTKFTRDTI
                     LPTDYTSPVFYDTTSTTVIVSAPWTTKSLSGSSGSKTFGVNLIAGMDYYFSKNLSLGA
                     EFGFGFYSTKYDDLKSDYLDVKNTWVESVPDANGVSTITAGQTTTTKSNPSMKQGKDS
                     GFGPNVTAKIKLGWLF"
     gene            complement(2868..4007)
                     /locus_tag="PK134_03945"
     CDS             complement(2868..4007)
                     /locus_tag="PK134_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRB38031.1"
                     /translation="MEFAATENQQMIAQTVKDFAEKHIRPNVMTWDEAQDFPVDVFKK
                     MGDIGLMGLLVPEQYQGAGLGYFEYVTAIVEVARVCGSVGLSMAAHNSLCTGHILAFG
                     NEEQKKKWLPKLATGQWIGAWGLTEANTGSDAMRMQCVAKQDGDSWIINGAKNWITHG
                     KSGDVAVVLARTGELLDSNGITAFVVERGTPGFKAGKKENKLGMRCSETAEMIFEDCR
                     IPASNILGKVGDGFKQAMKILDGGRISIAALSLGIAKGAFDASVKYAKERQQFGQPIA
                     NFQAIGFKLADMATKIEASELLILQAADLKNRGEKVTRQSAYAKYYASEVAVYVSTEA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVIARELLK"
     gene            4171..>5123
                     /locus_tag="PK134_03950"
     CDS             4171..>5123
                     /locus_tag="PK134_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB38032.1"
                     /translation="MMHATIEKGAYTASDMAETLIGSEIIKLAGEVNEQIKKGNKIFN
                     LTIGDYDPAVFPIPDALKNEIIKAYQNGYTNYPVANGQPELRNAVAGYINKLQKLQYS
                     SNEILISGGARPLIYALYKTVVNPGERVVFPIPSWNNNHYCHLSQAEAVAVETQPQNN
                     FMPLASELKSHLSSAALVALCSPLNPTGTVFSSSQLMEICELILQENKRREGKKPLYL
                     MYDQIYMALTFGKTKHVDPVSLLPEMRPFTIYIDGLSKAFSATGLRVGWAFGPVGIID
                     KMKSILSHVGAWSPKAEQVATAHFLTQEKEVDVFLSHYKSEV"
     assembly_gap    5124..5133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5162..>5347
                     /locus_tag="PK134_03955"
     CDS             5162..>5347
                     /locus_tag="PK134_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRB38033.1"
                     /translation="MKNDGLPVNAIDPQAAIYLTVQFDILNKVMPDGKRINNSNDITS
                     FLINYAKVALVPFSAFGA"
     assembly_gap    5348..5358
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5403..6293)
                     /locus_tag="PK134_03960"
     CDS             complement(5403..6293)
                     /locus_tag="PK134_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB38034.1"
                     /translation="MRKAYLQMHISILLWGFTGIFGKAIEMNAIMIVWYRMIISAVAL
                     IPFMVKHKQFILPDKKRLASIIITGFVVCLHWLTFYASIKASNVSIALSCFASVSLFS
                     ALLEPLFYRKKINKQNIILALFVLVGIYIIFAFQQLYAMGIVLALISALLGALFTILN
                     KIFVSNDEPAPVTFIELISGFVFLSVLLPLILPAFNVSFQIPSKIDWVWLLLLSVVCT
                     SIAFTLSLEALKKVSAFTMNLSVNLEPLYSIVLAIILFKENELLNNGFYIGAVIVISS
                     VFIHSLMQYRVRDKSVTEQG"
     gene            complement(6297..6869)
                     /locus_tag="PK134_03965"
     CDS             complement(6297..6869)
                     /locus_tag="PK134_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRB38035.1"
                     /translation="MNHLKQKIILASRSPRRQQLLQMMGIEFEVRTKETVEDYPEHLQ
                     NEQIVLYLASKKAIAFENEIQENELLISADTIVVHQNKVLNKPADFNEAIEMLTTLKG
                     NMHEVYTGVCIASIHKKELLYDRSEVYFRNYTTEDMHYYISNFKPYDKAGSYGIQDWF
                     GLTCIEKINGCFYNVMGLPTRKVYQQLINF"
BASE COUNT         2265 a         1323 c         1313 g         2190 t
ORIGIN      
        1 tgattgcagt gtgtggttta atgccgttgt taggggtgat gtacattcaa taacaattgg
       61 caataaagta aatattcagg atggtgctat tattcattgc acatatcaaa aagcaccaac
      121 agttattgga aataatgttt ctattggtca caatgccatt gttcatggct gtacacttga
      181 agataatgtg ctgattggta tgggtgcaat tgtaatggat catgcaatca ttaaaacaaa
      241 tgcaataatt gctgcaggtt ctgttgtgct ggaaaacaca gtggtagaat caggttgtat
      301 ctatgcggga gtgcctgcaa aaaaagtaaa agaagtaagc cctgaacatt ttaaagattt
      361 gaatgagcgt attgctaata actatgtaat gtattccggt tggtttaaaa aagacacgga
      421 tgaaaaatag agttatactt ttttggcttt ttttaaacat ttgttttctt tttttctcat
      481 gtgcaacacc tgagaaaaaa gaatcagtac aacaagttga ttcttcaaca gtagtgcaaa
      541 cacattttac caaaataaaa ttagaacccg gaaaacttct ttccaacgtc aagtcagaga
      601 gtaacagtaa tgtatctttt tctgtttatg caccatccga atatagcgat acaacattac
      661 ttcctataat ttattttttc gatcctcatg ccgatggaaa tttgccatta gaaaaataca
      721 aatcaattgc caatactttt aatctgatat tggttggttg caacagtatt gaaaacggaa
      781 tgccttttga acaatcacaa cagattgcca acattctttt ttctgattgt caaaacaggt
      841 tgcctataaa cacgcaacaa caaattattg caggattttc agggggtagt aaagtagctt
      901 gcaatcttgc aatgaatcaa cattttttta caggattgat agcctgttct ggagccttgt
      961 tcgaaaatgg caagtttgac aagaacatga gtgtagtttc agtagcaggt ataaatgatt
     1021 ttaattacca tgaaatggtt gcattagaaa aatctgttga cgtaacacac catgctttta
     1081 ttgaaacaga cagcacacat caatggccgg gcacaagcga tatgtatgat gccacgctct
     1141 ttacactgat gcaaatggtt aaacagcata aattacagat gcagagttct gttgtagaaa
     1201 atttttatca tacctggtta aagaaaatta acgattcagc aaacaaatct tctcccggaa
     1261 aacaatttga tcagttacaa tttggaatta tgtgttttga tggattgaca gatgtaacaa
     1321 gtctaaaaag taaacttaaa gaaattgaaa actccgataa cctacgtaag tggcgtatgc
     1381 gaagtcagaa tatgcagtat caggaaaagg aaatgcagaa gatgttacgc gatgcttttt
     1441 caaatcaaaa cctggcatgg tggcaaaaag aaattaccga tttaaaaact gcagctacta
     1501 atagtaatga caaaatgatg gcagccatga gtaaccgcct gttgggattt attggcattg
     1561 cctgttattc ttttgccaaa caattggtaa acgaaaatgc acaggagtca gataaagttc
     1621 tacaaatcta tcagacagtt gaaccgaata attcagaggc atggtttctt tcagcagttt
     1681 atcatgccaa caacaagcag cccgaaaagg caatgacaga ctttaataaa gctgtacaga
     1741 atggttttaa tcaaacatca aggattaatg agtatcctgc tctgcaacaa attgtagcgc
     1801 cctgatttga attttcattt ctgagttatt catattgaca tgtttataat tcatcggatg
     1861 agaatttatt atcggacttg cagtgaatac ttttacacca aacaaaaact gaaaaataaa
     1921 atgaaaagac taatgttatt agcaacaggc atgttcctgt gtctgacagc cgttgaggct
     1981 caaccttaca aacaggcagg tggagaaaac aacctccaac tattgtttgc accttttgga
     2041 agtacacctc tttcattgaa cgaaggaatc acttatcgca gatttttaaa agatggaaaa
     2101 cttgcagcaa gactgagtat ttctatcagc agttcaaaaa gcactgatgt tattatgcag
     2161 gcagatgata cacttgcatt cccaacaaca acaactgtgt ttcatgcaga aggtggaggt
     2221 ggctacattc aattaacgac agggatgaat ccacaagcag atttggtaag caaaacttca
     2281 gggtttagtt tccgtccggg tatagaaaaa cattttgaag gcacagacag gttatcacct
     2341 tatattggag cagaaattgt tttcagcagt tcaaaaacaa aattcacacg cgacaccata
     2401 ttgccaacag attatacatc accggttttt tatgatacta catcaacaac tgtaattgtt
     2461 tctgcacctt ggacaacaaa atctttaagt ggttcatcag gcagcaaaac atttggtgta
     2521 aacctgattg ccggtatgga ttactatttc agcaaaaatc tttcattagg tgctgagttt
     2581 ggttttggat tttacagtac taaatatgat gatttgaaat ctgattactt agatgtaaaa
     2641 aatacatggg tagagtctgt gcctgatgct aatggtgtaa gcactattac tgcaggacaa
     2701 actactacaa caaagagtaa tccttcaatg aaacaaggaa aggattcagg ctttggtccg
     2761 aatgttactg ctaaaataaa attaggttgg ttgttctgat aggaaaatca cctaaacaaa
     2821 aaaggctgct caattgagca gccttttttg tttgtgcttt aatcctatta cttcagcagt
     2881 tctctggcaa ttactaattt ttgaatttcg gaagtgcctt ctccaatggt gcaaagttta
     2941 ctgtcgcgat aaaatttctc taccggaaaa tctttggtat aaccatagcc gccaaaaatc
     3001 tgcacagctt ctgtgcttac ataaacagcc acttccgaag cataatattt tgcataagcc
     3061 gactgacggg taactttttc accacggttt tttaaatcag ccgcttgtaa tattaaaagt
     3121 tctgatgctt caatctttgt tgccatgtcg gccaacttaa aacctattgc ctgaaagttg
     3181 gcaatgggct gtccgaactg ttgtctttct tttgcatatt taacagatgc atcaaatgca
     3241 ccctttgcaa tacctaatga tagtgcagca atagaaatgc gtccgccatc caatattttc
     3301 attgcctgtt taaatccatc gccaacctta cctaaaatat ttgaagccgg aattctgcaa
     3361 tcttcaaaaa tcatttcagc agtttcgctg catcgcattc ctagtttatt ttcttttttg
     3421 cctgctttaa atcccggtgt tccgcgctcc accacaaatg ctgtaatgcc attggagtcc
     3481 agcaattcac cggtgcgtgc aagcactact gccacatcac cgcttttgcc atgagtaatc
     3541 cagttttttg caccattaat tatccaactg tcgccatctt gctttgccac acattgcatg
     3601 cgcatggcat cgctgccagt attggcttct gttagtcccc atgcacctat ccattgtccg
     3661 gtagccaatt taggtaacca tttctttttc tgctcttcgt ttccaaaagc taaaatgtgc
     3721 cctgtacata atgagttatg cgcagccatt gacaagccta ccgatccgca aactcttgct
     3781 acctctacaa tggcagtaac gtattcaaaa taacctaaac ctgcaccctg atattgctcc
     3841 ggtactaata gtcccataag cccgatatcg cccatctttt taaaaacatc aacaggaaaa
     3901 tcctgcgcct catcccacgt catcacattg ggtctgatat gtttttcggc aaaatctttt
     3961 accgtttgtg caatcatctg ctgattttct gtagctgcaa attccattct ctaaggtatt
     4021 tttattaatt gaattttttt tttcagaagt gtgcaaagat aaaaaaacaa acccattttt
     4081 agggtgatat tttattattc cggtaaatga gtaaacttgc attgttaact catattttct
     4141 gaatactttc ggagcaaatt tttgaatgaa atgatgcatg caacaataga aaaaggagca
     4201 tatactgcct ctgatatggc agaaacttta attggttcgg aaatcataaa actcgctggc
     4261 gaagtaaacg aacaaattaa gaaaggaaat aagattttta atctgactat tggcgattat
     4321 gaccctgccg tttttcctat tcccgatgcg ctaaagaatg aaattattaa agcctatcag
     4381 aatggatata ccaattatcc tgttgccaac ggccagccag agttgcgtaa tgctgttgca
     4441 ggttatatca ataaattgca aaaattacaa tacagtagca atgagatttt aatttccggt
     4501 ggggcacgac cacttattta tgcattgtac aaaacagttg taaaccccgg tgagagagtt
     4561 gttttcccta ttccatcatg gaacaataat cactattgtc atttgtcgca ggcagaagct
     4621 gttgctgtag aaacacagcc acagaataat tttatgccgc ttgcatcaga actcaaatca
     4681 catctgtcat cggcagcatt ggttgcactt tgttctcctc taaatcctac aggtacggtc
     4741 tttagcagta gtcagttaat ggaaatatgt gagttgatat tgcaggaaaa caaaagaaga
     4801 gaaggtaaga agccattgta tcttatgtac gatcagattt atatggcttt gacttttggt
     4861 aaaacaaaac atgttgaccc ggtatcactg cttcctgaaa tgcgaccttt cacaatatat
     4921 attgatggtc tttccaaagc tttttctgca acaggtttgc gtgtaggctg ggcatttggt
     4981 ccggtaggaa ttattgataa aatgaaatct attctctcgc atgtaggcgc atggtcgcct
     5041 aaggcagagc aggtggcaac agcacatttt ctgacgcaag aaaaagaggt agatgtattt
     5101 ttaagtcatt acaaatctga agtnnnnnnn nnngaatgca ttttatgatg gctttataaa
     5161 aatgaaaaat gatggattgc ctgtcaatgc cattgacccg caagcagcca tttacctcac
     5221 cgttcagttt gatattttaa ataaggtgat gcctgatggt aaacgtataa ataattcaaa
     5281 cgacattaca tcgtttctca ttaactatgc caaggtagca ttagtaccct tttctgcatt
     5341 tggtgcannn nnnnnnnngt atggaagata tagaacagat gtttgtttct ttacgcaaag
     5401 ctttagcctt gctcggtgac tgacttatct ctgacccggt attgcatcag actgtgaata
     5461 aaaacagaac tgatgacaat gactgcgcca atgtagaaac cgttattgag caattcattt
     5521 tctttgaata aaattattgc aagaacaatg ctgtaaagcg gttctaaatt tactgagagg
     5581 ttcatggtaa aagcactcac tttttttaat gcctccaatg aaagtgtaaa ggcaatagag
     5641 gtgcaaacta cactcagcaa caacagccac acccaatcta tctttgatgg aatttgaaaa
     5701 ctaacattaa aagccggaag gatgagtgga agaagaacag ataaaaacac aaatccactg
     5761 attaattcta tgaatgttac cggtgcaggc tcgtcattgc tgacaaatat tttattcaga
     5821 atagtaaaaa gggcaccaag caatgcactg attaatgcta gaacaatgcc catggcatat
     5881 aactgctgaa aagcaaaaat gatatagatg ccaacaagta caaacaacgc taagatgatg
     5941 ttttgtttat ttattttttt gcgataaaac aatggctcca gcaatgcaga aaatagtgaa
     6001 accgaagcaa aacaactaag tgcaattgac acattagaag cttttatgga ggcatagaag
     6061 gtgagccaat gcagacagac tacaaaacct gtgatgatga tggatgctaa tcttttttta
     6121 tcaggcagta taaactgttt gtgttttacc ataaatggta tcaatgccac tgcactgatg
     6181 atcattctgt accatacaat catgatagca ttcatctcaa ttgccttacc gaatattccg
     6241 gtaaatcccc acaacaaaat ggagatgtgc atttgcagat aagcctttcg catgaactaa
     6301 aaatttatta attgctgata aacttttctt gttggcaagc ccataacatt ataaaaacat
     6361 ccgttaattt tttctataca tgtcaatcca aaccaatcct gaattccgta agatcctgct
     6421 ttgtcatagg gcttaaaatt actaatgtaa taatgcatgt cttctgtagt gtagttgcga
     6481 aaataaactt cactcctgtc atagagcaat tcttttttgt gaatggaagc aatgcaaacg
     6541 cctgtataaa cttcatgcat gttgccttta agagtagtga gcatttcaat agcttcgtta
     6601 aagtctgccg gtttattcag cactttgttc tgatgtacaa caatggtatc tgcgctgatg
     6661 agtaattcat tttcctgaat ctcattttcg aaagcaatag ctttttttga agcaagatat
     6721 aagactatct gttcgttctg caagtgttcc ggataatctt caacagtttc ttttgttctt
     6781 acttcaaatt caatacccat catttgcaaa agctgctgtc tgcgtggtga acgtgaagcc
     6841 agaattattt tttgtttgag atgattcatt gcatcaggat tgatgtgcaa aggaaatggt
     6901 tttatgcttg catcggaatt tatactctta tgtgtattta cttaatcaac aattttttta
     6961 aatctttttt gcttccgtta aacacattgg catcataaga agaacttgaa ccgggaatag
     7021 aaacgcggtc ggagtgttgc cagaaaagcc attttaccga cttctcaatt tttaattcgt
     7081 cttcgtagta gtgtgcaatc caaaagttat ag
//
LOCUS       DAORQT010000072         7103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1619_length_7103_cov_10.692537, whole genome shotgun sequence.
ACCESSION   DAORQT010000072 DAORQT010000000
VERSION     DAORQT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1619_length_7103_cov_10.692537"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..82)
                     /gene="ffs"
                     /locus_tag="PK134_03970"
     ncRNA           complement(2..82)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK134_03970"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            164..682
                     /locus_tag="PK134_03975"
     CDS             164..682
                     /locus_tag="PK134_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRB38036.1"
                     /translation="MFRYGLHYGGILGASSFAMFLIIYLTGNNPLGNASWLGAWIPAV
                     VIAVAVKSYRTHEGEGFISYGEGFRTGLITALFGGILSALLIYIFCTLFDSTLVERFK
                     TDSLAQLELMEGQFKSMLSDKMYDQAVDAYNNISLQSIAMNDFYNKLLSGIILSLIIA
                     AVYKRNKPQILE"
     gene            702..1667
                     /locus_tag="PK134_03980"
     CDS             702..1667
                     /locus_tag="PK134_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB38037.1"
                     /translation="MDISVVIPLFNEDESLPELAAWIDRVMKQHKFSYEVIMVDDGSK
                     DHSWQVIEDLNKQIPEIKGVKFKRNYGKSAALHKGFEMAQGHVVITMDADLQDSPDEI
                     PGLYKMIVEDEYDLVSGWKKKRHDPISKTIPSKFFNGVTRMVSGIDNLHDFNCGLKAY
                     HHNVIKNIEIYGEMHRYIPVIAKWVGFTKIGEKVVEHRARKFGTTKFGLERFVNGFLD
                     LLSITFVSRFGKRPMHLFGTLGSLMFILGLIGACYLGVSKLYCVYNDIPATRLTQRPS
                     FYLLLTSMILGTQLFLAGFLAEMIARNDKDRNRYIVEKEISNTTT"
     gene            1686..3626
                     /locus_tag="PK134_03985"
     CDS             1686..3626
                     /locus_tag="PK134_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38038.1"
                     /translation="MKLSVVIVNYNVKYFLEQCLISVGKAISNIDAEVFVVDNNSVDG
                     SCQMVRDKFPFVNVIENKINTGFSKANNQAIVQSKGEYLLLLNPDTVVEEDTFKKVVD
                     FMDNHPQAGGLGVHMIDGNGKFLPESKRGLPTPAVAFYKIFGLSALFPKSKLFGQYHL
                     GFLDKNKTNEVDVLSGAFMLLRKSVLDKIGLLDETFFMYGEDIDLSYRITKAGYKNYY
                     FPETKIIHYKGESTKKSSVNYVVVFYKAMSIFAQKHFSQSNAAFFSFLINIAIYLRAG
                     IALMNRLFSILLLPIIDAAFIYGGYTIIKTYWELYVKQLHYPEHYMLLVVPGYVLVWI
                     FAAYLNGGYDKPLRISRVVRGILSGTIFILVVYALLNETYRFSRALILMNTAWACLIT
                     ALIRYTLNAAGIKGFALAADVKKKLIVVADAVDAKRILSLLQLSGSNTTFVGYVDSGV
                     TQDGELKNFRLGTLTQLNEIITIYQVDEVIFSSHSLSSQQIIENMGALRNRQVEYKIA
                     PPESLFIIGSNSINDNGDLYLVDVNTLHSPSNRRKKRFFDIFSAFGILFCYPFLWLLF
                     KKNQNVFVNSIAVLFGKKTWVATNIKSLTSTHAKVGVYTPSDGLNTPSDNEYFRDRLN
                     VLYTRDYNMINDWNIVRRNIFK"
     gene            complement(3634..4722)
                     /locus_tag="PK134_03990"
     CDS             complement(3634..4722)
                     /locus_tag="PK134_03990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB38039.1"
                     /translation="MIQLKSQEVINAPGRNSSVDIFRSFAILSVVIYHYNRQLPFGYL
                     GVDLFFVISGLLIGNILIGYLKKGEKINFFKFIIQRGFKIWPSYFSYLFIGSIIANVL
                     YHTLAPDMIIPADKYLRYILFYVNYTGNEVWSFGHLWSLCVEEHFYIMLPLLLILIQR
                     FFPDKKMLVTAILLVITAGVIFKFLVMAFTNSKDTYSATHNRIDALGWGVMLSIILNF
                     YPKLIVKQSTQITMLIAGIILFSLLLYTYSAYEWITFRKCVFHSAIPFCFFLMLAATY
                     HWNMSGLNWLRFIAYYSYNWYLWHPLWVTWFNKKIGTGLMAHLVFLIFTFLTAMAFTI
                     FIEEFFMGKRNKFISKWFGERKIQVKTL"
     gene            complement(4730..5491)
                     /locus_tag="PK134_03995"
     CDS             complement(4730..5491)
                     /locus_tag="PK134_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRB38040.1"
                     /translation="MKELIRFLKSRIFWINALVIIIVIIMSIGLVYKWLDSFTDHGNS
                     VSVPDLKGMNIKKVDDFLKTKNLSFKIADSSVYLLDQKPGTIVEQDPQPNEKVKQGRT
                     IYLTITRSSAPMVKVPALKDVSLRQAEAILAASGLRMGEQIFKPDLAKNAVLSMMING
                     KDLKAGTDVPKGSAIDLIVGDGLGNTVVTVPSLIGLTYDEALFVLKGSSLNIGSLFFD
                     GVIKDTLNAKIYDQNPAPDNNTINQGEAIDLYLRP"
     gene            complement(5496..>7103)
                     /locus_tag="PK134_04000"
     CDS             complement(5496..>7103)
                     /locus_tag="PK134_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRB38041.1"
                     /translation="IHLPEVQIETIRRECERLFTSLNFSVYARIDGFITENGTIFLND
                     PNTTSGMMPSSFFFHQAAEIGLNPSQFISYIISTSLQARLLEMKSFRTVPQLIKNLHG
                     LLSKKAKSQSEKIKVGVIMGGYSSERHISVESGRNVYEKLSSTEKYEPIPYFLAGNEQ
                     SYSLYRIPINLMLKDNADDIREKILNYNQHPVIKKIIDQCGQVTQTFANAANINPPEK
                     TSFEELAQQVKIVFIALHGRPGEDGELQLQLNKVGLPFNGSRAESSKITIDKFQTNEL
                     LAQNGFKVAQHYKLTEEEWRNNPSAIEEKIQKEFGFPIIAKPVDDGCSSAVKIIRNEN
                     QLHAYAELIFREDETLNTAAAELLKIKDKEEFPRKRYFLIETLISGENTKHFLEITGG
                     MLTSYDEAGNIHYEIFEPSETLHEGDVLSLEEKFLAGEGQNITPARYSKHAMENRLIS
                     DIIRKELLRAARLLNIEGYARIDAFVRITIDNKVEVVFIEVNSLPGMTPATCIFHQAA
                     INQYKPYEFIEKILEFGFKRNQLSLTK"
BASE COUNT         2232 a         1211 c         1315 g         2345 t
ORIGIN      
        1 acttatatcg caccgctacg accacttatc cttgctgcat tcctgccctg gggaggttcg
       61 agggagctgg tcgtataaga cttacccggt gcaaagatag taaatcatac ctttcaataa
      121 acaaataaac acagcaggtt ttataccttt gcaatcataa attatgtttc gatacggatt
      181 acattatgga ggaatattag gggcatcaag ttttgctatg tttttgatta tttatcttac
      241 gggaaataat ccgcttggca atgcatcgtg gctcggtgca tggatacctg cagttgtaat
      301 tgctgttgca gtaaaaagtt accggacaca tgagggtgag ggttttatct cttatggtga
      361 aggtttccgt acaggattga ttactgctct ttttggtgga attctttcag cattgttgat
      421 ttatattttc tgcaccttat tcgacagtac attggtagag cgttttaaaa ctgattctct
      481 tgcacaactt gaacttatgg aaggacagtt taaatcaatg ctcagtgata aaatgtatga
      541 tcaggctgtt gatgcctaca ataatataag tcttcagtca attgcaatga acgattttta
      601 taataagcta ctcagcggaa ttatactttc acttatcatt gccgcagtgt ataaacgtaa
      661 caagcctcaa attctcgaat aaaatgcagt catcatacag aatagatatt tcagtagtta
      721 ttccgttgtt taatgaggat gagtctctac ctgaattggc agcatggatt gaccgtgtta
      781 tgaagcaaca caagtttagc tatgaagtga ttatggttga tgatggtagc aaagatcact
      841 catggcaggt gattgaagac ctaaataaac aaatacctga aataaaaggt gtgaagttta
      901 aaagaaacta tggcaagtct gctgcactac ataaaggttt tgaaatggca caaggtcatg
      961 tagtaattac aatggatgcc gacctacaag acagtcctga cgaaattccc ggactttata
     1021 aaatgattgt tgaagatgaa tacgatttgg tgagtggatg gaaaaagaaa cgtcacgatc
     1081 cgatttcaaa aactattccc agcaaatttt ttaatggtgt cacccgtatg gtttccggga
     1141 ttgataatct gcacgatttt aattgtgggc ttaaagcata tcatcataat gtgattaaga
     1201 atattgaaat atacggtgag atgcatcgtt atattcctgt aattgcaaag tgggttggct
     1261 ttacaaaaat cggtgagaag gttgttgaac acagagccag aaaatttggt actacaaaat
     1321 ttggattaga aagatttgtt aatggatttc ttgatttgct ttccatcaca tttgtttcac
     1381 gttttggtaa acgccccatg catttattcg gtactttagg ttctcttatg tttattctgg
     1441 gacttatagg tgcttgttat cttggtgttt caaaacttta ttgcgtttat aatgatatcc
     1501 ctgcaacacg attaacacag cgcccatcat tttatttatt gctgacaagt atgattctgg
     1561 gaacacaact ttttcttgca ggatttcttg cagagatgat tgctcgcaat gataaagacc
     1621 gcaatcgtta tattgtggaa aaagaaatat ctaacacaac aacgtaaccc aaaaattctg
     1681 ttgcattgaa gctatccgtt gtcatcgtca actacaatgt aaagtacttt cttgagcaat
     1741 gcctcatttc tgttggtaag gctattagta atattgatgc agaagttttt gtggttgaca
     1801 acaattctgt tgacggatca tgtcagatgg ttcgggataa atttccattt gtaaacgtaa
     1861 ttgaaaataa aataaacacc ggtttttcaa aagcaaacaa tcaggctatt gtacaaagca
     1921 aaggtgagta tttgctactt cttaatccgg acactgttgt agaggaggat acatttaaaa
     1981 aggtggttga ctttatggac aatcatccac aggcaggcgg acttggagtg cacatgattg
     2041 acggaaacgg aaaatttttg cctgaatcca aaagaggact tccgacacct gcagtggcat
     2101 tctataaaat cttcgggctg tctgcattgt ttccaaaatc aaaattattt ggtcagtatc
     2161 atttaggttt cctcgacaag aataagacca atgaagttga tgtgctcagt ggtgcattta
     2221 tgttgttgag aaaatctgtg ttggataaaa taggattact tgacgagaca ttttttatgt
     2281 atggtgaaga tattgacttg tcatacagaa taacaaaagc cggctataaa aattattatt
     2341 tccctgaaac aaaaatcata cactacaaag gagagagtac caagaagagc agtgtaaatt
     2401 atgtagtagt gttttataag gcaatgagca tttttgctca gaaacatttt tcgcagagca
     2461 atgctgcatt tttttcattt ctgataaata ttgccatcta cctaagagca ggtattgcat
     2521 tgatgaatcg cctttttagt attttgttgt taccaattat tgatgcagca tttatatatg
     2581 gaggttatac cattattaaa acatattggg aactttatgt caaacaattg cattatccgg
     2641 agcattacat gcttttagtt gtgcccggat atgttttagt ctggattttt gctgcttatc
     2701 tgaatggtgg ttatgataaa ccactgcgta tttccagagt tgtaagagga attctgtcag
     2761 gcacaatttt tattttagtt gtgtatgctc tgttgaacga gacctaccgt ttttcaagag
     2821 cgctcatttt aatgaataca gcctgggcat gtctgataac tgcgttaatc cgatatacgt
     2881 taaatgcagc aggtattaaa ggatttgctt tggcagcaga tgtcaaaaag aaattgattg
     2941 ttgttgcaga tgctgttgat gcaaaaagaa ttttatcatt gctacaatta tcagggtcaa
     3001 atacaacttt tgtaggatat gttgattcag gagtcactca ggacggagaa ttgaagaatt
     3061 tcagacttgg aacattgaca caattaaatg aaatcattac tatttatcag gttgatgaag
     3121 taattttcag ttctcactca ttaagctcgc agcagataat cgagaatatg ggggcgctgc
     3181 gtaacaggca ggttgagtat aagattgctc ctccagaaag tttgtttatc attggtagta
     3241 attcaataaa tgataatggt gatctttatc ttgttgatgt aaatacatta cacagtcctt
     3301 ccaatagaag aaaaaaaaga ttttttgata ttttcagtgc tttcggaata ttattctgct
     3361 atccttttct ttggctatta ttcaagaaaa atcaaaatgt ttttgtaaat agcattgctg
     3421 tactttttgg aaagaaaaca tgggtagcaa ccaatattaa gagtttaacg tcaacacatg
     3481 ctaaagttgg tgtttatacg ccatcagatg gattaaacac accatcagac aatgaatatt
     3541 tccgcgacag attaaatgtg ctttataccc gtgattacaa tatgattaac gactggaata
     3601 tagtacggag aaatattttt aagtgagaaa atattacaag gttttaacct gaatttttct
     3661 ttcgccaaac catttactga taaacttatt tcttttaccc atgaaaaatt cctctataaa
     3721 aatagtaaat gccattgctg tcagaaaagt aaaaataaga aacaccagat gtgccatcaa
     3781 acctgttccg atttttttgt taaaccaggt tacccaaagt ggatgccaca aataccagtt
     3841 gtaactgtag tatgcaataa atctcaacca atttaatcct gacatattcc agtgataagt
     3901 agctgcaagc atcagaaaaa aacaaaaggg tatagcagaa tgaaacacgc atttcctgaa
     3961 ggtaatccat tcataagcac tatatgtgta cagaagtaaa ctaaaaagaa tgattcctgc
     4021 aatcagcatt gtgatttgtg tgctttgttt tactattaac ttaggataaa aattgagaat
     4081 aatactcagc atcacacccc atcctaaagc atctatgcgg ttatgtgtgg cagaataggt
     4141 atctttacta ttggtaaaag ccatgacaag aaacttaaat ataactcctg ctgtaattac
     4201 caataagatt gcagtcacca acattttttt gtcggggaag aatctttgaa ttagaatcaa
     4261 tagcagtggt agcatgatat aaaaatgttc ttctacacag agcgaccaca aatgcccaaa
     4321 actccatact tcattaccgg tataattaac atagaagaga atataacgta aatatttatc
     4381 tgccggaatt atcatgtcgg gggcaagggt atgatataaa acgtttgcta tgattgatcc
     4441 tataaacaga tacgaaaaat aagatggcca aattttaaac cctcgttgaa taataaattt
     4501 gaaaaaattt attttctccc ctttttttaa atagccaata agaatattac ctatcagtaa
     4561 tccggagatc acaaaaaata aatcaacgcc aagatagcca aaaggaagct gcctgttgta
     4621 atgataaata acaacactta gtatcgcaaa tgatctgaaa atatctacac ttgaatttct
     4681 gccaggggca ttaattacct cttgtgattt tagttgaatc ataaaagaat caaggtctca
     4741 aatataaatc tatggcttca ccctgattga tggtgttgtt atcaggtgca gggttctgat
     4801 catagatttt tgcattaagc gtatctttaa tcacaccatc aaaaaataat gaaccaatgt
     4861 ttaatgatga tccttttagc acgaataatg cttcgtcata agttaggcca ataagagatg
     4921 gaacagtgac tactgtattt cctaatccat caccaacaat caaatcaatg gcagaacctt
     4981 taggtacatc tgttcctgct tttaaatctt ttccatttat catcatggag agcactgcat
     5041 tttttgcaag atcaggtttg aaaatctgct cacccattct aagtcctgat gcagcaagaa
     5101 tggcttccgc ctgtcgtaag gaaacatctt tcagtgccgg aactttcacc attggtgcac
     5161 tactgcgagt tattgtaaga tagatagtac gaccttgttt tactttttca tttggttgcg
     5221 gatcttgctc aactatagtt cccggttttt gatctaaaag gtaaaccgat gaatcagcaa
     5281 ttttaaatga caagttttta gtctttaaaa aatcatcaac ctttttaatg ttcatgcctt
     5341 ttaaatcagg cacagaaact gagttgccat gatctgtaaa actgtcaagc catttgtaaa
     5401 ccaaacctat tgacatgatg attacgataa taatcaccaa agcattaatc cagaaaatgc
     5461 gcgacttaag aaatcgaatt agttctttca ttattttatt tcgtcaggga taactgattt
     5521 cttttaaatc cgaattcaag tattttttca ataaactcat aaggcttata ctgatttatt
     5581 gcagcctgat ggaaaatgca agttgccggt gtcatgccgg gcaatgaatt cacttctata
     5641 aaaacaactt caactttatt gtcgattgta atacgaacaa atgcatcaat tcgtgcatag
     5701 ccttcaatat ttaacagtcg tgctgcacgc aaaagttctt ttctgattat atcagaaatc
     5761 agtctgtttt ccattgcatg tttgctgtaa cgtgctggag tgatattttg tccttcaccg
     5821 gctaaaaatt tttcttcaag cgaaagtaca tcaccttcat gtaatgtttc gctgggttca
     5881 aaaatttcgt aatgtatatt tccggcctca tcataggatg tgagcatgcc acctgtgatt
     5941 tctaaaaaat gtttagtgtt ttcaccactg ataagtgttt caataaggaa atatcttttt
     6001 cgtggaaact cttctttgtc ttttatcttc agtagttcag ctgcagcagt gtttaatgtt
     6061 tcatcttcgc gaaaaattaa ttcggcatag gcatgtaact gattttcatt tcggataatt
     6121 tttactgcac tactgcatcc atcatcaacc ggtttagcaa taatagggaa tccaaattct
     6181 ttttgaattt tttcttctat cgcagatgga ttattacgcc attcttcctc tgtgagttta
     6241 taatgttgtg caactttaaa tccattttgt gccagcaact catttgtttg aaatttatca
     6301 atagtgattt tagaagattc agcccttgag ccattgaatg gtaaaccaac tttattaagt
     6361 tgtaattgta attcaccatc ttcacccgga cgaccatgta aagcaatgaa aacaattttt
     6421 acttgttgcg ccagctcttc aaacgatgtt ttttcaggag gatttatatt ggccgcattt
     6481 gcaaacgttt gtgttacctg accacattgg tcaattattt ttttaataac aggatgctga
     6541 ttgtaattga gaattttttc tctgatatcg tcagcattat cttttagcat caggtttatt
     6601 ggaatcctgt aaagtgaata agactgttcg ttaccggcaa gaaaataagg aatgggttcg
     6661 tacttttctg ttgaagataa tttttcataa acatttcgtc cgctctcaac actaatgtgt
     6721 ctttcactgc tatagccacc cattatgaca ccaactttta tcttttcaga ttgcgatttg
     6781 gcttttttgc tgagaagccc atgcaggttt tttatcagtt gtggtaccgt acgaaatgat
     6841 ttcatttcta agagacgcgc ctgaagtgat gttgaaataa tgtagctgat aaactgagaa
     6901 gggttgagac cgatttctgc agcctgatga aagaaaaagc tactgggcat cattcctgat
     6961 gttgtattcg gatcattcaa aaatattgta ccattctctg taataaaacc atctattcgg
     7021 gcataaacac taaagtttaa tgacgtaaac agacgttcac actctcttct tattgtttca
     7081 atttgtactt cgggcaggtg aat
//
LOCUS       DAORQT010000073         7079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1627_length_7079_cov_8.014095, whole genome shotgun sequence.
ACCESSION   DAORQT010000073 DAORQT010000000
VERSION     DAORQT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_7079_cov_8.014095"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK134_04005"
     CDS             <1..711
                     /locus_tag="PK134_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38042.1"
                     /translation="STMSSKKIKYTLLTIFVLLSCSGNVHAQLRDTLAYLFKQRPKPF
                     FQLDGYNSFVRSRGANAIGFKTGLDFGKRVKFSMGYMKLFTDVVDSVQVKPNKYYRGE
                     VRSNYFTTGIEYVIYRNGPWQVNVPMHFGFGTSYYEYPDGKKANNKALQGNIILFEPA
                     ITGHYKLIRWVGLGFGVGYRVMLKNNHQLKDNLSSPIYILRLKIFVDEIYRTVFPPKQ
                     KNKNKIWIQFLAVIHWRK"
     gene            672..1106
                     /locus_tag="PK134_04010"
     CDS             672..1106
                     /locus_tag="PK134_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRB38043.1"
                     /translation="MDSIFGSYTLEEVKLMGKNTMLNHIGIEITSIKNNTIKGKMPVD
                     NRTRQPKGIMHGGASCVLAESLASLGAFLTLKDKSATCVGMEINANHVRPVKEGFVYG
                     TAKPFHLGKKTQVWTIEILNEKQQPVCISRMTIAIGRLHLKI"
     gene            complement(1247..1792)
                     /locus_tag="PK134_04015"
     CDS             complement(1247..1792)
                     /locus_tag="PK134_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRB38044.1"
                     /translation="MLEQLLNLVKENAGEAIINNQAIPNEQNDAAISQATEGIIGGLK
                     SQLASGNISEVAQFLGGQNNAATTGVTNAISSQVSQGLMEKFGLSGNSAGSIVSSLIP
                     VVLSKLVSKTNDPNDKSFDLNSIFNHLSGGKTQGMDLSNILGSVTGGNASGGGIGNVL
                     GSVMGGDKNSALGGLMDIFKK"
     gene            1963..2886
                     /locus_tag="PK134_04020"
     CDS             1963..2886
                     /locus_tag="PK134_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRB38045.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKIIGGAATYASLAASYFAPKIN
                     LVGVVGDDFPQSTIQDFNNHNIRTEGLQIIEGEKSFFWSGRYHTDMNSRDTLDTQLNV
                     LANFDPIIPESYQDCEFLMLGNLMPKIQLQVIERLRKRPKLIVMDTMNFWMDMAMDDL
                     LKNISMIDVLTINDAEARQLTNEYSLVKAAKKILNMGPKYLIIKKGEHGALLFHNNEV
                     FFAPALPLEDVFDPTGAGDTFAGGFIGYLAKTRDISFSSMKRAIIYGSAMASFCVEKF
                     GTERIMNLDSDELNERIQEFIDLVHFDITLV"
     gene            2962..3504
                     /locus_tag="PK134_04025"
     CDS             2962..3504
                     /locus_tag="PK134_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRB38046.1"
                     /translation="MSILFHSMNILDLFLAVPLLWGAYKGFTRGIIYEIAMILGLIAG
                     IYVAFKFSGVVQGWLFQLVNKDSALPAWVGFFIISGIIMAVFILYARLLEGILKAGDL
                     NLFNKIIGGVFGLLKYALVLSVFLWLLKSLEPHLNFINSQTKKESKLYDPVLKTSTFL
                     TPALQDIKNEFRQHIEDNNH"
     gene            complement(3523..4713)
                     /locus_tag="PK134_04030"
     CDS             complement(3523..4713)
                     /locus_tag="PK134_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB38047.1"
                     /translation="MLDKYNLPVELKYLAIVESALNPTAVSSAGATGIWQFMFNTGKM
                     YGLNINSYIDERRDVRLATDAACRYFQDMYAIYGDWLLVIASYNCGPGNVNRAIARAG
                     GSKNFWEISRFLPAETRGYVPAFIGATYVMYYAKDYNITPTKPSFTYFDVDTVGVKNK
                     ISFRQLSQTLNIPYDVIAFLNPVYKRNMIPGEGNRLYLPTEKISMYLANEEGLYAASG
                     TELQPSSFAIADDVGYVQVKQHVKVRKGENLFTFAKRNDCSAKQVKKWNGLRSNNIKA
                     GQKLVVYATIKQKPAPVENTTATAANDKTDSANVNTTVKADSSSDSETGAVTTESKTT
                     QVSQAQSAASKYIIHLVQPGDTLWNIAKRYEGVTVELLKEANNFTPKSTLKVGSKIKV
                     PTES"
     gene            complement(4810..5082)
                     /locus_tag="PK134_04035"
     CDS             complement(4810..5082)
                     /locus_tag="PK134_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38048.1"
                     /translation="MRLKKLVYSIALLLPFIGNAQVVENAQPQSGDPIAAMLDSLVSI
                     RNVVRLSETNLQQPPVFSENDAVLSDAFIQERISKISTPIPLTYNY"
     gene            complement(5230..6300)
                     /locus_tag="PK134_04040"
     CDS             complement(5230..6300)
                     /locus_tag="PK134_04040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRB38049.1"
                     /translation="MINIAFSCITAFIITFLAIPSIIKVAVIKNLFDEPGGRKAHKAS
                     VPTLGGLAIFAGVVFAFTFWSAGFNLQDTQYIIAALIVMFFIGIKDDIVELPAMKKLY
                     GQIVAAVIIVLFANIRITGLYGIFGIDQVPYWFSLLFSMFTILLIINAFNLIDGVDGL
                     AAGIGAIAAFTYGIYFYNYNEIALCILSFSLFSSLLAFLVYNFAPANIFMGDTGSLII
                     GLILSILTIKFIEMSFAPRPENFPFQSSPAMAVAILVLPLFDLLRVFIVRILNKRSPF
                     QADRLHIHHLLLDMGLNHREVSITLYLVNIGFILVALALRSFGALALLFVISSLALIL
                     SLVPYYLKYQAGRSGAVSRSTK"
     gene            complement(6409..>7079)
                     /locus_tag="PK134_04045"
     CDS             complement(6409..>7079)
                     /locus_tag="PK134_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38050.1"
                     /translation="VEFSIGSPDNMMLGFNLKVKLFSKLVFYGQLMLDEFKLSEIRDG
                     NGWYANKQAFQLGLKSYDLPIKNLDVLTEFNYVRPFTYSHRTTFTNYAHFNQSLAHPL
                     GSNFYESMSLINYKYKNFIFRFQFNYAETASDTGNANLGNDIFKSYETYTFAYGNRML
                     QGQRHNLIYSDIRAGYLINPRTNLCFEIGYSYRRMTTNSEAATTNWVYAGIRTALFNR
                     YHDF"
BASE COUNT         2248 a         1523 c         1275 g         2033 t
ORIGIN      
        1 tcaaccatgt cttccaaaaa aataaaatac acgctgctca ccatattcgt tctgttatca
       61 tgcagtggca acgtgcatgc acaactacgc gacacgttgg cctatttatt taaacaaaga
      121 ccaaaacctt tttttcagtt agacggctac aactccttcg ttcgcagccg cggtgccaat
      181 gccatcggtt tcaaaacagg tttagatttc ggcaaacgtg taaaattcag catgggctac
      241 atgaaactgt ttactgatgt ggttgacagc gttcaagtaa aaccaaataa atactatcgt
      301 ggcgaagtgc gaagtaatta ttttacaaca ggcattgaat atgtaatcta ccgcaacggc
      361 ccctggcagg tcaacgtacc catgcacttt ggcttcggca caagctacta cgaatatcct
      421 gacggaaaaa aagcaaacaa caaagctttg cagggcaaca tcattctctt cgagcctgcc
      481 atcaccggac actataaact catccgttgg gtgggcctgg gctttggcgt gggctatcgt
      541 gtcatgctca aaaacaacca tcagctaaaa gacaatctat catcaccaat atacattctg
      601 cgacttaaaa tttttgtgga cgaaatctac cgcacggttt ttccacctaa acaaaaaaat
      661 aaaaataaaa tatggattca atttttggca gttatacact ggaggaagta aaactgatgg
      721 gaaaaaacac aatgctcaac cacataggca tcgaaatcac ctccataaaa aataacacta
      781 taaaaggcaa aatgcctgtt gacaaccgca cacggcaacc aaaaggtatc atgcatggtg
      841 gtgcatcatg tgttcttgca gagtcgctgg caagcttggg tgcatttctc acactcaaag
      901 acaaaagcgc aacctgtgtg ggtatggaaa taaatgctaa ccacgtgcga cctgttaaag
      961 aaggcttcgt ctatggtact gcaaaaccct tccaccttgg aaaaaaaaca caagtctgga
     1021 caatagaaat tctaaacgaa aaacagcaac ccgtttgcat cagcagaatg accattgcca
     1081 tcggacgttt gcatttaaag atttgagttt tgaaagttgg aatgtttgaa agttgaaata
     1141 tttgggtgta gatttaaaaa tttagaatta aaaaatccct tcagtaaaat caatttgata
     1201 aaaccgaagg gattaataat taatgttgat actaaggtgt ttttccttac ttcttaaaaa
     1261 tatccatcag tccacctagt gcagaatttt tatctccacc catcacagag ccaagcacat
     1321 tacctattcc gcctccgctt gcattgccac cagtcacaga acccaatatg ttcgacaaat
     1381 ccattccctg tgttttgcca ccactcaaat gattaaaaat actattcaaa tcaaaactct
     1441 tatcgttagg atcattcgtt ttagaaacca acttactcaa cacaacaggt atcagtgacg
     1501 acactataga acctgcactg tttcccgaca aaccaaattt ttccatcaac ccctgcgaca
     1561 cctgcgatga aatggcattc gtaacacccg ttgttgctgc attattctgt ccgcctaaaa
     1621 attgtgctac ttcactaatg ttgcccgatg ccaattgcga tttcaatccg cctataatgc
     1681 cttccgtagc ctgactaata gctgcatcat tctgttcatt cggaatagcc tgattgttaa
     1741 tgatagcctc tccggcattt tcttttacaa gatttaataa ttgttctaac atgattttta
     1801 tgtattaaaa gattaaggtt taaagataca tctatctcct tattttttta atgaagaaat
     1861 attagctcgt gtaattgcat tgccgcaaca actcatttac aaaacacaat cacaaaaacc
     1921 gataatagta ttagctttgc gcaaaatttt caaactcaca tcatgtcatt actcgttgtt
     1981 ggaaccgttg ccttcgatgc aatagaaaca cccttcggaa aaaccgataa aataattggt
     2041 ggtgctgcca cctatgcctc acttgcagct tcctactttg ctcctaaaat aaatcttgtt
     2101 ggcgttgttg gcgatgactt tccgcaaagc accattcagg actttaacaa ccacaacata
     2161 cgcacagaag gtctgcagat tatcgaaggc gaaaaatctt tcttctggag tggccgctat
     2221 catacagaca tgaacagccg cgacacctta gatacacaac tcaacgtcct tgccaacttc
     2281 gaccccatta ttcccgaaag ttatcaggac tgcgaattcc tcatgctcgg taacctcatg
     2341 cccaaaattc agttgcaggt aatagagcgt ttgcgcaaac gtccaaaact catcgtcatg
     2401 gacaccatga acttctggat ggatatggcc atggacgatc tgctcaaaaa catttcaatg
     2461 attgatgtgc tcaccatcaa cgatgccgaa gcacgacaac tcaccaacga atattctttg
     2521 gtaaaagctg ccaagaaaat tctaaacatg ggaccaaaat atctcatcat caaaaaaggc
     2581 gaacatggcg cactcctctt ccacaacaac gaagtatttt ttgctcctgc actgccatta
     2641 gaagatgtgt tcgaccccac aggtgctggc gacacttttg ccggtggctt cattggctat
     2701 cttgcaaaaa cccgcgacat ctctttcagc agcatgaaac gtgccatcat ctacggttct
     2761 gcaatggcat ctttctgtgt agaaaaattt ggcacagaaa gaatcatgaa cctcgatagt
     2821 gacgaactca acgaacgcat tcaggagttt attgatctgg ttcattttga tattacattg
     2881 gtttaaccaa cacctttcaa caatttattg tttggtattt tctgctgatg ctgtttgtag
     2941 attacaacag catcgcttac tttgagcata ctattccata gcatgaatat tctcgatttg
     3001 tttttagccg tgcccttatt atggggagca tacaaaggtt tcactcgcgg cattatttat
     3061 gagattgcca tgatactcgg tctcattgcc ggaatctatg ttgcattcaa attttcaggc
     3121 gttgtgcaag gctggctctt tcaattagta aacaaagaca gtgccctgcc tgcatgggta
     3181 ggcttcttta ttattagcgg aatcattatg gcagtgttta ttctttatgc ccgtctgctc
     3241 gaaggtattc tcaaagcagg cgacctcaat cttttcaata aaattattgg tggtgtattt
     3301 ggtttgctta aatatgcttt ggtgctgagt gtgttcctgt ggctgctcaa atctctcgaa
     3361 ccacatctca actttatcaa tagtcagaca aaaaaagaat ctaaactcta tgatcctgtc
     3421 ctaaaaacct caacattcct cacacctgca ctgcaggata ttaaaaacga attccgtcag
     3481 catattgaag acaacaacca ttaaaaaaac cgaaattgaa aattaactct ctgtagggac
     3541 ttttatttta cttcctactt taagtgttga tttaggtgta aaattatttg cctctttcaa
     3601 tagctctaca gtaacacctt catagcgttt tgcaatattc caaagtgtat cacccggctg
     3661 cactaaatga ataatgtatt ttgatgcagc actttgtgcc tggcttacct gagttgtttt
     3721 gctctcagtt gtaacagcac ctgtttcaga gtcagatgat gaatctgctt taactgttgt
     3781 atttacatta gccgagtctg ttttatcatt tgcagcagtt gcagtggtat tctcaaccgg
     3841 tgcaggtttt tgctttattg ttgcatagac aactaatttc tgtcctgctt taatattgtt
     3901 cgaccttaaa ccattccact tctttacttg tttggcagag caatcatttc tttttgcaaa
     3961 ggtgaagaga ttttcgccct tacgaacttt tacatgttgt ttcacctgca cataaccaac
     4021 atcatcagca atagcaaacg atgatggttg caactctgtg ccacttgcag cataaagacc
     4081 ttcttcatta gccaggtaca tacttatctt ttctgttggc agataaagtc tgttgccctc
     4141 accgggaatc atgttgcgtt tgtaaacagg attcaaaaat gcaatcacat cataaggtat
     4201 gtttagcgtc tgactcaact gacgaaacga aattttattt tttacaccaa cagtgtccac
     4261 atcaaaataa gtgaatgaag gttttgtggg cgtgatgtta taatctttag cataatacat
     4321 cacataagta gcaccaataa aagccggtac ataacctctt gtttctgcag gaagaaaacg
     4381 acttatctcc caaaagtttt tgctgccgcc tgcacgcgca atagcacgat tcacatttcc
     4441 cggcccacag ttgtacgatg caatcaccag caaccaatcg ccataaatag catacatatc
     4501 ctgaaaatag cgacatgctg catctgttgc caaacgcaca tctctgcgct catcaatgta
     4561 ggagttaatg ttcaggccat acatttttcc ggtgttgaac ataaactgcc aaatacctgt
     4621 tgcacctgct gacgatactg ctgtcgggtt aagtgccgat tcaacaattg ccagatattt
     4681 taattcaaca ggaagattgt acttgtccag catttgttca aaatacggaa agtacaaacg
     4741 cgataaacac attacacgtg aagtcagtgg tgcccgttgt gaggcataaa gatttatgta
     4801 gtttttaacc taataattat aggttaacgg tataggtgtc gaaatttttg aaatcctttc
     4861 ctgaataaaa gcatcagaaa gcacagcatc gttctcacta aaaacaggag gctgctgaag
     4921 gtttgtttcg ctcagtcgca caacattacg aatagacacc aaactgtcca acatggcagc
     4981 tatagggtca ccggattgag gttgtgcatt ttcaactacc tgagcattac ctatgaacgg
     5041 caataaaaga gcaatggagt aaaccagttt ttttagtcgc atgataatta atttagcagg
     5101 cagagagtcc ggaaaagtgc ctgttattca agttcggacg aaaataaaaa aaatgtttac
     5161 tttttgggga aatgcttaca taattatatc aatttttcat gtaagtgagg caaatagggt
     5221 attaatgccc tacttggttg accttgaaac tgcccctgaa cggcctgcct gatactttaa
     5281 gtagtagggc accaagctca aaatcaaggc taaagagcta ataacaaaca acaatgccaa
     5341 tgcgccaaag ctgcgtaatg ccaatgccac aagtataaag cctatgttca ccaagtaaag
     5401 tgtaatggaa acttcgcggt gatttaaacc catatcgagc aacaggtgat ggatatgaag
     5461 tctgtctgcc tgaaaaggtg aacgcttgtt taaaattctg acaataaata ccctgagcaa
     5521 atcaaacaaa ggcaacacca aaatggcaac tgccatagcc ggactactct gaaatggaaa
     5581 gttttccgga cgtggtgcaa aactcatttc aataaacttg atagtgagga tagataaaat
     5641 caacccaatg attaaagaac ccgtatcgcc cataaaaatg ttggcaggtg caaaattata
     5701 aaccaagaaa gctaataacg aactgaataa tgaaaacgaa agaatacata gagctatctc
     5761 attatagttg tagaaataaa ttccataagt aaaagctgca atagcaccaa tgcctgctgc
     5821 cagtccgtca acaccatcaa tcaagttaaa agcattgatg ataagcagaa tggtaaacat
     5881 tgaaaacaaa aggctgaacc aatatggcac ctgatctatt ccaaaaattc cataaagacc
     5941 tgtaatgcgg atgttggcaa acaagactat aataacagct gcaactatct gtccgtaaag
     6001 ttttttcatt gcaggaagtt caacaatgtc atcttttatt ccgataaaaa acatcactat
     6061 caaagctgca ataatgtact gtgtgtcctg aaggttaaac cccgcagacc agaaagtaaa
     6121 tgcaaaaaca acaccggcaa aaatggctaa gccaccaagg gttggaacac ttgccttatg
     6181 tgccttacgt ccgcccggtt catcaaaaag attttttatt acggcaacct taataattga
     6241 cggtatggca agaaaggtaa taataaaagc ggtaatacaa ctaaaagcaa tattaatcat
     6301 ggtgcgtaat ctgtttggct caaatgtatc tctcaatatt tactacaaag gtactatttt
     6361 gccaatagca gtttttttca ggtaaaacaa ctgtaaatat aggtgtaatt aaaaatcatg
     6421 ataacggtta aaaagtgctg tcctgatacc tgcataaacc cagtttgtgg tggctgcttc
     6481 actattggtt gtcattctcc tatagctata accaatctca aaacataagt tggtacgtgg
     6541 gttaattaag tatcctgctc tgatgtcgct ataaatcagg ttgtgacgtt gcccctgaag
     6601 cattctgtta ccataagcaa aagtgtaggt ttcgtagctc ttgaaaatgt cgttgcccaa
     6661 atttgcattt cctgtatcgc ttgcagtctc agcatagtta aactgaaaac ggaaaataaa
     6721 atttttatac ttgtagttaa tcaaagacat cgactcataa aagtttgatc ctaaaggatg
     6781 tgccagcgac tgattaaaat gtgcatagtt tgtaaaggtt gtacggtgcg aataagtaaa
     6841 cggccttaca tagttaaact ctgtcagcac atcaagattt ttaatcggca aatcataact
     6901 ctttaatcct aactgaaatg cttgcttgtt agcataccat ccattgccgt ctcttatttc
     6961 cgatagttta aattcatcca acatcaactg tccgtaaaat accaatttcg aaaacaactt
     7021 tactttcaga ttaaaaccca gcatcatatt atctggcgaa cctattgaaa actctacag
//
LOCUS       DAORQT010000074         7000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1665_length_7000_cov_7.460907, whole genome shotgun sequence.
ACCESSION   DAORQT010000074 DAORQT010000000
VERSION     DAORQT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_7000_cov_7.460907"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /gene="carB"
                     /locus_tag="PK134_04050"
     CDS             <1..1325
                     /gene="carB"
                     /locus_tag="PK134_04050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRB38051.1"
                     /translation="EVYETRKEMGVKRIYKMVDTCSAEFAAKTPYFYSTFERPAQDSN
                     GNEINASESIVSNKKKILVLGSGPNRIGQGIEFDYSCVHGVLASKESGYEAIMVNCNP
                     ETVSTDFDIADKLYFEPVYQEHIFELIEYEKPDGVIVQLGGQTALKLSEYLNEKGIKI
                     IGTSYEDMDLAEDRGRFSDLLHDLGIPYPPYGVAESAEEALVVANKVGYPVLVRPSYV
                     LGGQGMSIVINDEELERAVIKLLKNLPGNRVLIDHFLDRAEECEADLICDGKDVQIIG
                     IMEHIEPAGIHSGDSYAVLPPFSLSDVVISQIKDYAHKLALALNVKGLLNIQYAVKNE
                     KVYVIEANPRASRTVPFIAKAHQVPYINIATKVMLGVKKLKDFNIKPAEFGYAIKEPV
                     FSFEKFADVNKELGPEMKSTGEAIRFIESTNDAYFRNLYKEKSMYLSK"
     gene            1343..1543
                     /locus_tag="PK134_04055"
     CDS             1343..1543
                     /locus_tag="PK134_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38052.1"
                     /translation="MRDVFYTIVVVWVVIQIYKSFIKPKSNTTQRNTTRQEGDVTINT
                     NTNNNNRNNDQGEYVDFEEIKD"
     gene            complement(1546..2706)
                     /locus_tag="PK134_04060"
     CDS             complement(1546..2706)
                     /locus_tag="PK134_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB38053.1"
                     /translation="MSGTVLAIAVTSIALSVMVMLVAVAIVTGFKKEITSKVVGFGAH
                     IRIKHFDNNQSYEEIPVNIDQKFINKLKSDNRISSVNAYATKAGIIRTSEDILGVAVK
                     GLDSTFNSGFFNPNMIEGSFNLQIHSGIYPAIISKSVADKMKLKLNDSFVVYFIDEPP
                     RARKFKVNGVYSTGLEEFDNLYVFCPLSVIQKLNNWNPLQAGGYEVNLKQLSDLESVN
                     ESVYKEAGFELNAQSIKTIYPEIFHWLDLQDVNVIIILVLMILVAGINMISTLLIILL
                     DNTTTIGLLKAIGAGNDGIRKIFLMVSSGIVGIGLIAGNVAGLLLCWLQYQFHLVSLP
                     QESYYVPYVPINLSVTNILLINAGTLVACILVMLIPSMIVSRISPVKALRYE"
     gene            2806..3987
                     /locus_tag="PK134_04065"
     CDS             2806..3987
                     /locus_tag="PK134_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRB38054.1"
                     /translation="MLLKRWIVLLSIIISHQSTAQMLDSVITGAGQINNYLPIISDKN
                     VAVVANQTSIVGQTHLVDLLLSKHVKVKKIFTPEHGFRGVAEAGENIESGKDKSTGLP
                     IISLYGNHKKPTTDDLKGIDIVLFDLQDVGTRFYTYSSTLQYVMEACAENKKKLIVLD
                     RPNPNGYYVDGPVLEPEFKSFVGLNPIPVVHGLTMGEYANMLNGEGWLANKEQCDVTV
                     ISVKGYTHETRYKLTVNPSPNLPNMTAVYLYPSLCFFEGTIVSIGRGTTYPFQVVGYP
                     GFTEGPFEFTPKKNPEMKLPPLYENQKCRGFDLRDFGNYYIPSLKKLYLFWIKNIYDN
                     YQQKDKFFNDYFDKLAGTDKLRKQIIAGLNEEQIRRTWEEGLKSYKVIRKKYLLYPDF
                     E"
     gene            4089..5016
                     /locus_tag="PK134_04070"
                     /pseudo
     CDS             4089..5016
                     /locus_tag="PK134_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
     gene            5036..5638
                     /locus_tag="PK134_04075"
     CDS             5036..5638
                     /locus_tag="PK134_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRB38055.1"
                     /translation="MKRNIVIFLILLMTSTAHAQELWDDVAIDSLPVFHSLNAAMQNP
                     DSVFRLDLSKQKLKEIPAQVFELTQLRELNVSRNHLTELPEQVYKLNNLQRLYANNNK
                     LTALPQRIGTLKNLQTLTLDRNRIEALPPEIGMLHNLEVLSLWDNELSEIPDEIKSLS
                     SVLKKLELRGILFNEEQQKRIHDLLPYTTIYFSPACNCKQ"
     gene            5671..6189
                     /locus_tag="PK134_04080"
     CDS             5671..6189
                     /locus_tag="PK134_04080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRB38056.1"
                     /translation="MLQTDKLQLRACEPDDLPLMYQWENNSDYWLLSDTTKPFSNYTI
                     SEFIKQDQNDIYTTKQQRFVMELRNPVKPIGFIDLYQFEAVHRRAGVGILIGDLSEQK
                     KGYAAQSLECLKNYSHTVLNMHQLYCYIQSSNKKSLALFQQAGFKECGVLKDWLINKN
                     KPEDVVMFQFIF"
     gene            6274..6669
                     /locus_tag="PK134_04085"
     CDS             6274..6669
                     /locus_tag="PK134_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRB38057.1"
                     /translation="MQTLSETINKVKEFHATFGLQYFEKPQAAIDKNIIALRHKLMQE
                     ENDEYLEAAMNNDLEQIADALGDKLYILCGTIIAHGLQHKIADVFNEIHRSNMSKLDE
                     NGKPIYREDGKIMKGNNYFLPDIKAVLEQ"
     gene            6666..>7000
                     /locus_tag="PK134_04090"
     CDS             6666..>7000
                     /locus_tag="PK134_04090"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB38058.1"
                     /translation="MIQKLSIIIPVFNESETIISVLEKVSDVQLINNIAKQIIIVDDH
                     SSDNSLEAITQFKNKHPQADITIVKQQVNMGKGYAVRTGMSKATGDYIIIQDADLELN
                     PDDFNILLKT"
BASE COUNT         2364 a         1238 c         1315 g         2083 t
ORIGIN      
        1 aagaagttta tgagacccga aaagaaatgg gagtaaaacg tatttacaaa atggttgata
       61 cctgctctgc agagtttgca gcaaagacac cctatttcta cagcactttc gaaaggccgg
      121 cacaagacag taatggcaat gaaattaatg caagtgaaag tattgtcagt aataagaaga
      181 aaattttagt cttaggcagt ggccctaacc gtattggtca gggtattgag ttcgactatt
      241 catgtgttca tggtgtatta gcatcaaaag aatcgggcta tgaagcaata atggttaact
      301 gtaatcccga aactgtaagt acagattttg acattgcaga caaactttat tttgaacccg
      361 tatatcagga acacatcttc gagcttattg aatatgaaaa accagatggt gtaattgttc
      421 aacttggtgg acaaacggct ctgaaactat cggagtatct caatgaaaaa ggaataaaaa
      481 taatcggcac ttcttatgaa gacatggatt tggcagaaga ccgtggacgt ttttctgatc
      541 tacttcatga tttaggaata ccctatcctc cttatggtgt tgcagaaagt gccgaagaag
      601 cattggttgt tgccaataaa gttggatatc cggttttggt tcgtccaagt tatgtgttgg
      661 gtggacaagg aatgagcatt gtaatcaatg acgaagaatt agaacgtgca gtaataaagc
      721 tgctgaaaaa tcttccgggt aacagagtgc tcattgacca ttttcttgat cgtgcagaag
      781 aatgtgaagc agatttgatt tgtgatggca aagatgttca gattataggt atcatggaac
      841 atatagaacc ggcaggcatt catagtggtg attcttatgc agtgttacct ccattcagtt
      901 taagcgatgt tgtcatcagt cagataaaag attatgcaca taaacttgca ttggcactta
      961 atgtaaaagg cttattgaat attcagtatg ctgttaagaa tgaaaaagta tatgtgattg
     1021 aagccaatcc acgagcatca agaacagttc cttttattgc caaagcacat caggtgccat
     1081 acattaacat tgcaactaag gtaatgcttg gtgtaaaaaa attaaaggac ttcaacatta
     1141 aacctgctga atttggatat gcaataaaag agcctgtgtt ttcgtttgaa aaatttgctg
     1201 acgttaataa agagttaggc cccgaaatga aatctacagg tgaagccata agatttattg
     1261 aaagtactaa tgatgcctat ttccgtaatc tatacaagga aaaaagcatg tatttatcaa
     1321 aataatttta caatagcaga tcatgcgtga tgtcttttat acaatagttg ttgtctgggt
     1381 ggtgattcag atttataaaa gttttataaa acctaaaagc aatacaactc aaagaaatac
     1441 taccagacag gaaggcgatg tgacaataaa cacaaatact aataacaaca atcgcaacaa
     1501 cgaccaaggc gagtatgttg attttgaaga aataaaagac tgattttact cgtagcgcag
     1561 tgctttcaca gggcttattc gcgaaacaat cattgacgga ataagcatca ccaatataca
     1621 tgcaactaaa gtacctgcat taatcagcag aatattagta accgataaat ttattggaac
     1681 ataaggaaca taatatgatt cctgtggcag actaaccaaa tgaaattggt attgtaacca
     1741 gcacaaaaga agtcctgcaa catttcctgc aatcaatcca atgccaacaa ttcctgaaga
     1801 aaccatcaga aaaatttttc ttattccgtc atttcctgca ccaatggctt tcaacaaacc
     1861 aattgttgtt gtattgtcta ataagataat caagagtgta gaaatcatat tgattcctgc
     1921 aaccaatatc atcaaaacaa gaatgatgat aacattgaca tcctgcaagt caagccaatg
     1981 aaatatttca ggataaattg ttttgatgga ttgcgcattc aattcaaaac ctgcttcttt
     2041 atagacactt tcattgacag attcaaggtc gctgagttgc tttaaattta cttcatagcc
     2101 tccggcctgc aatggattcc agttgtttaa tttctgaatt acagaaagtg ggcaaaaaac
     2161 atagaggtta tcaaactctt caagccctgt ggaataaact ccattgactt taaacttgcg
     2221 ggctcttggc ggctcatcaa taaaataaac tacaaaagaa tcattcagct ttaatttcat
     2281 tttgtctgct acactttttg aaattatagc aggataaata ccagaatgta tctgaagatt
     2341 gaaacttcct tcaatcatat tcggattaaa aaaacctgag ttaaaagttg aatctaatcc
     2401 ttttacagct acacctaaaa tatcttcaga agtgcggatt attccggcct tggtggcata
     2461 ggcatttaca ctgctgattc tgttatcact ctttagcttg tttataaact tctgatcaat
     2521 atttacagga atctcctcgt aggattgatt gttatcaaaa tgttttattc tgatatgtgc
     2581 gccaaagcca acaaccttag aggtaatttc ttttttaaaa cctgtgacta ttgcaacagc
     2641 aacaagcatc accattacac ttagtgcaat gctggttaca gctattgcca gaacagtgcc
     2701 tgacatacgt tggttgttaa cactgcctgt gaaaaaacgc tttgctatga agtaagagta
     2761 tctcaactaa tttattattc ttgctgatat ttcggtttca aatttatgct tttgaagcgt
     2821 tggatagttt tactatccat aattatcagc catcaaagca ctgctcaaat gcttgacagt
     2881 gtaattaccg gtgccggaca aatcaataat tatttgccga tcatcagtga taagaatgtt
     2941 gctgtggttg ccaatcagac aagtatagtt ggccaaacac atttggttga cttgcttctt
     3001 tccaagcatg ttaaagtcaa gaaaattttc actcctgagc atggttttag aggcgttgcc
     3061 gaagcgggag aaaacattga atccggtaaa gataaaagta caggcctgcc cattatttct
     3121 ctttatggta atcataaaaa gccaacaact gatgatttaa aaggtattga tattgtactg
     3181 ttcgacttgc aggatgttgg tacacgtttt tacacctaca gttcaacatt acaatatgtt
     3241 atggaggcct gtgcagaaaa caaaaagaaa ctgattgttt tagacagacc taatcctaat
     3301 ggatattatg ttgatggccc tgtgcttgaa cctgaattta aatcttttgt tggccttaac
     3361 cctattcctg ttgtacacgg actcactatg ggtgagtacg caaacatgct gaatggtgaa
     3421 ggttggcttg ccaataaaga gcaatgtgat gttactgtta tcagcgtcaa aggatatacg
     3481 catgaaacaa gatataaact gactgttaat ccttcaccca atttaccaaa catgacagcc
     3541 gtttatctgt acccgtcctt gtgttttttt gaaggaacaa ttgtgagtat cggccgcggt
     3601 actacctatc catttcaggt tgttggctac cccggtttta ctgagggccc gtttgaattt
     3661 acacctaaaa aaaatccgga aatgaaactt cctccgctct acgaaaatca aaagtgcaga
     3721 ggctttgacc tgcgtgattt tgggaactat tacatcccat cacttaaaaa actatacttg
     3781 ttttggatta aaaatattta cgacaactat cagcaaaaag ataaattctt taacgattac
     3841 ttcgacaaac ttgccggcac agacaagctg cgcaaacaga taattgccgg cctgaacgaa
     3901 gaacagataa gacgaacctg ggaagaaggc ttgaagtctt ataaagttat tcgaaaaaaa
     3961 tatttactct atcccgattt tgaatagcga ttacaatttg gttttttcaa ataaacaagt
     4021 ggctttatca gtcatggttt taacgctcgg ctattctttt gtatttttgc actttatcca
     4081 cagtattttt gaaaatatat aaccatatag accattttga aaaggtcagc aatgccgtaa
     4141 taacacttgg aacatttgat ggtgttcatc ttgggcataa agtaattatt gaaagactga
     4201 aaaaaattgc cgtagatgtt aacgggcagg ttatagtact tacatttttt ccgcaccccc
     4261 gaatggtttt atttcctgac gaccacggaa ttcaactgct caacacaatt gatgaacgaa
     4321 ctatactgat ggagaaagct ggaatagatc atcttatcat tcaccctttt tcgaaagaat
     4381 tttcaagaat gagttctact gaatttgttc gcgatatttt agtcaacaaa cttggtgtaa
     4441 aaaaactgat catcggctac gaccatcatt ttggacgcaa cagagaaggc tcttttactg
     4501 atttggaaga attggcaccg gtttataatt tttctgtaga aagaattcat gagcaattag
     4561 tcaatgatgt tgcagtaagc tcaacaaaaa tcagaaacgc cttgctggca ggcgatgtgg
     4621 caactgcccg tgagttttta ggatatgatt atgagttaac aggaactgtt gttcatggca
     4681 aaaaaattag gtcacagcct tgggtttcca acggccaacc tacatatacg cgaggattac
     4741 aaattggttc cggcaaatgg catttatgca gcccgaacct tggttggtgg tcaatggcgt
     4801 gatggtgtga tgagtattgg aaacagacct acattcgaca atggagcacg ttctatagag
     4861 gtaaatttat tcgattttaa ttccgatatt tacggtgcaa agattaccat tcgtatgtcg
     4921 gattttatac ggagtgagaa gaaatttgaa aacagtgcag ccctgataga agccatgaat
     4981 gaagataaat tggctgcaat taaactttta tcgtaaaatt tacgttaaac gggtcatgaa
     5041 acgcaacatc gtcatatttc tgattttgct catgacatct acagcacatg cacaagagtt
     5101 gtgggacgat gttgcaatag attcattacc tgtatttcat tcactgaatg cagctatgca
     5161 aaatccggat tcagttttca ggcttgacct atcaaaacaa aaattgaaag agatacctgc
     5221 gcaggttttt gaacttacac aactgcgtga actgaatgtc agcagaaatc atttaacaga
     5281 attgccggag caagtttaca aactcaataa cctgcaacgt ttgtatgcaa acaacaacaa
     5341 actaacagca ttgccacaac gcatcggaac tttaaaaaat cttcagactt taacacttga
     5401 ccgcaatcgc attgaagcat tgccaccgga aataggaatg ttgcataatc ttgaagtttt
     5461 aagtctttgg gataatgaac tgtctgaaat acctgatgaa ataaaaagtc tttcaagcgt
     5521 attgaaaaaa ttagaattaa ggggaattct ttttaacgaa gagcaacaaa aaagaataca
     5581 cgacttgttg ccttatacaa ccatctattt ttcgcctgcc tgcaactgca agcaataaac
     5641 aagtcagtct gattatatat ttttgcctat atgttacaga cagataaatt acaattgcgt
     5701 gcctgcgaac ctgatgacct gccattaatg tatcagtggg aaaacaactc tgattattgg
     5761 ctgcttagtg acactacaaa gccattttcc aattacacca tttcagaatt tatcaagcaa
     5821 gatcagaacg atatttatac tacaaaacag cagcgttttg taatggaatt acgtaaccct
     5881 gtaaaaccaa ttggatttat tgacctctat cagtttgaag ccgttcacag acgtgcaggt
     5941 gtaggaattt taattggcga cctgagtgaa caaaaaaaag gatatgctgc acaaagtctt
     6001 gaatgtttaa aaaattattc gcatacagtt ttgaatatgc atcagcttta ctgctacatt
     6061 cagtcatcaa ataaaaagtc attagcactc tttcaacaag caggatttaa agaatgtgga
     6121 gtattgaaag attggctcat taataaaaat aagcctgaag acgtagtaat gttccaattt
     6181 atattctgat tactttttat taacagttgt tcgtcacacc caagcaaatt gaactttttt
     6241 gttcagtttt gctacgctac taaatcaaac aaaatgcaaa cattatcaga aaccatcaat
     6301 aaggtaaagg agtttcatgc aaccttcgga cttcaatatt ttgaaaagcc acaagctgca
     6361 atagataaaa acattattgc tcttcgtcat aagctgatgc aggaggaaaa tgatgaatat
     6421 cttgaagctg ccatgaacaa tgatcttgaa caaattgctg atgcactggg agacaagctg
     6481 tacattctat gcggcaccat cattgctcat ggattgcaac ataaaattgc tgatgtattt
     6541 aatgaaatac atcgcagcaa tatgagtaag cttgacgaaa acggaaagcc aatttacaga
     6601 gaagatggta aaataatgaa aggtaacaac tattttcttc ctgatattaa agcagtattg
     6661 gaacagtgat tcagaagctg agcattataa ttcccgtatt taatgaatct gaaacaataa
     6721 tttctgtatt ggaaaaagtt tctgatgttc aattaataaa caacatcgcc aaacaaataa
     6781 ttatagttga tgaccattca agtgataata gtttagaagc tataacgcaa tttaaaaata
     6841 aacatccaca agctgatatt acaattgtta aacagcaggt caatatggga aaaggttatg
     6901 ctgtgcggac aggtatgagt aaagcaacag gagattacat tatcattcag gatgcagacc
     6961 ttgaattaaa tcctgatgat tttaatattc tattgaaaac
//
LOCUS       DAORQT010000075         6954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1686_length_6954_cov_11.287143, whole genome shotgun sequence.
ACCESSION   DAORQT010000075 DAORQT010000000
VERSION     DAORQT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_6954_cov_11.287143"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2234)
                     /locus_tag="PK134_04095"
     CDS             complement(<1..2234)
                     /locus_tag="PK134_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRB38059.1"
                     /translation="MKSIITIVLLVLINANAFAQSEKYQKVRIWLEGKTVSSLAQTGV
                     DLSETHYRKGLYFETDLSTSELNSVSNAGFRTEVLIDDVKQFYKQRNEQQNQKTSAVM
                     SCSSAPDFPQPAHFGYGSMGGFFTYQELLNNLDSMASLYPNLITVKQPISNTLTTVEG
                     RPIFYVKISDNPNLTEPEPEVLYTALHHCREPNGMAAVIYYMWYLLENYATNTDIQQL
                     VNNTEMYFVPCVNPDGYVFNEMTDPNGGGMWRKNRQQFPGGEYGVDLNRNYGVQWGYD
                     DVGSSPDPADDTYRGPAAFSENETQMISAFCQQHNFKLALNYHTYSNLLIYPWGYIPD
                     YYTPDSALYKNYGDILTTYNKYAAGTANQTVGYMVNGSSDDWMYGEQTLKPKTFAMTP
                     EIGSADDGFWPQQSRIVDLCKENMYANVMLARLAGKYGQATDKSKWNIPALTSYIPFD
                     FKLLGLDTVGTFTVSLTPLSANIQTTGAAKTFSGLNFNLSVTDSITITLNSTTLPGDE
                     VKFLLTVNNGLYDISDTITKYYGPVVTPFFENGNTTSAWNTQFWGTATSSFISALSSI
                     ADSPIGDYQSSSINEITTISNINLLNAAAARLTFFTKWATEPQYDYAQLQISDNNGST
                     WTPLCGKYTVPGSPFQDVGDPVYQGVNLNWVKEDINLNAYTGKNVKLKFIMVSDGFVE
                     YDGIYVDDLSVEKIVASGVGINNLENNTFNIYPNPATDIARIETAANAKNINVINTLG
                     QTVFT"
     gene            complement(2336..3685)
                     /gene="tilS"
                     /locus_tag="PK134_04100"
     CDS             complement(2336..3685)
                     /gene="tilS"
                     /locus_tag="PK134_04100"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRB38060.1"
                     /translation="MLLQFQQFVKEKKLFSKEQTILVAVSGGIDSMVLLQLLLQSGYK
                     IAVAHCNFKLRGEESDGDAQFVHSFCLENKIVIHSTEFETEKLAKQNQQSIQGTAREL
                     RYNFFQELCSSFHYDFIATAHHADDVAETVLINLSRGGGLAAWHGIEVKRENIVRPLL
                     FAQKHEIEEYAKQNKIQWRTDSSNQKEDYTRNLIRHQIIPVLKKINPSVVQTLFHHAE
                     IVADAEQIIHEHIEKFRAASVVTDENKTIVPISDLLNTSAAQTLLFYILQPYGFNATH
                     TVDIFKSLNHSESKTFYSDSFQLVKEREQLVIIPFEKKRISKIYFEIPTTGTFSIADS
                     QISVEVLENTEDLYHIILKNNNQVIGYFDADKIEFPIIVRTWQHGDYFFPLGMHGKKL
                     VSDFFTDEKMSQSEKQKQLLFLSSNEVIWLEGRRISEKFKVEKNSKSVLKISVKAIV"
     gene            complement(3714..4985)
                     /gene="pabB"
                     /locus_tag="PK134_04105"
     CDS             complement(3714..4985)
                     /gene="pabB"
                     /locus_tag="PK134_04105"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRB38061.1"
                     /translation="MSDTKFSPAEFEELLKSSNGFRNFCFLNSNNAAEGSYSGLTKYD
                     FLAGINALDTVEATSESLEKLETFIQKNKGKWMFGFMSYELKNEIEQLKSSHTDRLNF
                     PVLHFFVPQVVLVSEGGKLRMIDQLEQTSKEFVADDVELRQMNASANVSLKPRFSNDD
                     YIQAVEKLKRQLQQGNIYEVTFCQEFYNDAANINPFETYIKLNTNTRAPMSCFYKKEN
                     QFIISSSPERFMCRRGNKIYSQPIKGTAPRSASREEDEWLKDNLQNSEKERAENLMIV
                     DLVRNDLSRIAKPGTVEVNELLSVYTFKTVHQLISTVSCEVEPKINFSEIIRAAFPMG
                     SMTGAPKISAMTLIDEHEKTNRGLFSGSVGYIDPSGDFDFNVIIRTILYNAQRNYVSV
                     QTGGAITIQSDPAAEYEECLLKAKAMMDVLG"
     gene            complement(5100..6185)
                     /locus_tag="PK134_04110"
     CDS             complement(5100..6185)
                     /locus_tag="PK134_04110"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRB38062.1"
                     /translation="MNQIIQLLFPFISSLIIASLIIPSWIQLCSKWNLFDRPDTRKHH
                     TAIIPSLGGIGIFIAIMISIFLFGYNNQDSSFHSIAAAMLILFITGFFDDLTDLKARV
                     KLNIQLLAALIVTYSGLRIQTLNGFTGIHELPIIAQYGISIFIIIFLVNAYNFIDGLD
                     GLAATIGLIIFSIFTVLFHIHGQQAYAVLCVSVIGALVSFLYFNFNPARVFMGDTGSL
                     VVGFLIAICTLKLFNLWFTSPVVSFGPSLTIATIFILIFDLTRVVFIRVTNGISPFKA
                     DRSHLHHMICRQQFGHRGGTFIMAAFNILFIVLVLPLSKLRFITFLVFCFCVAILLMN
                     SKVMSYVALLRNKITGAPESKMGVDRG"
     gene            complement(6195..>6954)
                     /locus_tag="PK134_04115"
     CDS             complement(6195..>6954)
                     /locus_tag="PK134_04115"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRB38063.1"
                     /translation="AGMISELSLSNNSLVKLGLLYRNNDAMVAYAGYQFNKIFLGTSY
                     DFNISKYKPGGSGAYEISLVYTPQGRAKGKRNEERKPEPVNKKKLPEQRVTPPQVLTP
                     IPVIAPSIIEQNPIVVSLAEMPMKGKSSMKEKTELSNYNYQTIGYVVFQDNSLKYDIN
                     NRFDVIEATIDYLYKNTNRKIIVSGNASEIEIKKNNSIGKQRAETVKDYMIGKGIDAD
                     RILIADVKDKMPALMIEDGIQVERLSRADILLIK"
BASE COUNT         2231 a         1313 c         1076 g         2334 t
ORIGIN      
        1 gtaaatactg tttgtcccaa agtattgata acattaatat tttttgcatt agctgcagtt
       61 tcaattcttg caatatctgt tgcaggatta gggtaaatat tgaatgtgtt gttttcaaga
      121 ttgtttattc caacacctga agctacaatt ttttctacac ttaaatcatc tacataaata
      181 ccatcatatt caacaaagcc atcacttacc ataataaatt tcaacttcac attttttcct
      241 gtataggcat tcagattaat atcttctttt acccaattta aattcacacc ctgataaaca
      301 ggatcaccta catcctgaaa aggacttccc ggtactgtat attttccaca aagtggtgtc
      361 catgtgctac cattgttatc ggaaatttgc aactgtgcat aatcatactg cggttctgtt
      421 gcccactttg taaaaaatgt caaacgtgca gcagcagcat tcaacaaatt aatattactg
      481 atagttgtga tttcattaat tgatgatgat tgataatcgc caataggaga gtctgctatg
      541 cttgatagtg ccgagataaa agacgatgta gctgttcccc agaattgtgt attccatgct
      601 gatgtggtat ttccattttc gaaaaacggt gtcacaacag ggccataata ttttgtaatg
      661 gtatcggaaa tatcatataa gccattgttt acggttaaca ggaattttac ttcatcaccg
      721 ggtaacgttg ttgaatttaa tgtgatggtt attgaatccg taaccgatag attaaaattt
      781 aaaccactga atgtttttgc tgctcctgtt gtttggatgt tagcagacag tggcgttaac
      841 gaaacagtaa atgttccaac agtatctaat ccaagtaatt taaaatcgaa tggtatatat
      901 gaagtaagtg caggaatatt ccacttgctt ttatcagtag cctgaccgta tttacctgcc
      961 agacgtgcaa gcataacatt ggcatacata ttttctttgc acaaatcaac aatgcgtgat
     1021 tgttgcggcc agaagccatc atctgcacta ccaatctctg gtgtcattgc aaatgttttt
     1081 ggctttaatg tttgttcgcc atacatccag tcatcagaac ttccattaac catataacct
     1141 actgtttggt ttgcagtacc tgctgcatat ttattatatg ttgttaaaat atcaccatag
     1201 tttttataca aagcactgtc aggtgtataa tagtcaggaa tatatcccca tggataaatc
     1261 aataaattac tatatgtatg ataatttaat gccagcttaa aattatgttg ttggcaaaaa
     1321 gcacttatca tttgtgtttc attctcactg aaagccgcag ggcctcgata agtatcatct
     1381 gcagggtcgg gcgatgaacc aacatcatca taaccccact gaacaccata attacgatta
     1441 agatcaacac catattctcc acccggaaat tgttgtctgt tttttcgcca cataccccca
     1501 ccattgggat cggtcatttc attaaacaca tatccatccg ggttaacaca gggcacaaaa
     1561 tacatttcag tattatttac taactgctga atatcggtat tggtagcata gttttcgagc
     1621 agataccaca tgtaatatat cactgctgcc ataccattag gctcgcgaca atgatgcaat
     1681 gctgtgtaaa gtacttcagg ttcaggctcg gttaaattgg ggttatcaga aatttttaca
     1741 taaaaaatag gacgaccttc aaccgttgtt aacgtattgc ttattggttg ttttacagtg
     1801 attaaattag gataaagcga tgccattgaa tcaagattgt ttaacaactc ctgataagta
     1861 aaaaatccgc ccatgctacc ataaccaaag tgtgcaggtt gcggaaaatc aggtgctgag
     1921 ctgcatgaca ttactgctga tgttttctga ttttgctgtt cattacgttg cttataaaat
     1981 tgttttacat catcaattaa tacttctgtt ctaaagccgg cattgctgac actatttaat
     2041 tcagaagtac ttaaatctgt ttcaaaataa agtcccttgc gataatgtgt ctcactaaga
     2101 tctactcctg tttgtgcaag tgatgaaaca gtcttccctt caagccagat tcgtactttc
     2161 tgatatttct ctgactgagc aaatgcgtta gcattgatta acactaacaa cacaattgta
     2221 attattgatt tcattttaat tgaataagat tttatattgc ttttttgata tggcctttac
     2281 actaacaaaa ctatctaaaa aaatctttac tcaaagtaat tttaacctgt ttgttttaaa
     2341 ctatagcctt aacactaatt tttaaaaccg acttactgtt tttctccact ttaaattttt
     2401 cacttatacg tctgccctca agccatatca cttcattact acttaaaaac aacaattgtt
     2461 tttgcttttc cgactgactc attttttcat cagtaaaaaa atctgatact aactttttgc
     2521 catgcatacc taatggaaaa aaataatcgc catgctgcca tgtccttaca ataattggaa
     2581 attctatttt atctgcatca aaataaccga ttacttggtt gttgttcttt aatattatat
     2641 gatacaagtc ctctgtattc tccaaaactt ctacacttat ttgagaatca gcaatagaaa
     2701 aggttcctgt tgttggaatt tcaaaataga ttttactaat cctcttcttt tcaaatggta
     2761 ttataacaag ttgttctcgc tcttttacca attgaaaact gtcagaataa aaagtcttac
     2821 tttcagaatg atttaaggat ttaaaaatat caactgtatg tgttgcatta aatccatacg
     2881 gctgaagaat ataaaacaat aaggtttgtg ctgctgatgt atttaacaaa tctgaaatcg
     2941 gaacaatagt tttattttca tcagtaacta cagatgctgc tctgaatttt tcaatatgct
     3001 catgaataat ttgttcggca tctgctacta tttctgcatg atgaaacaat gtctgtacaa
     3061 cagaaggatt tatcttcttt aatacaggaa taatctgatg tctgatgaga tttcttgtat
     3121 agtcttcttt ctgatttgag ctgtcagtgc gccattgaat tttgttctgt ttagcgtatt
     3181 cttcaatctc atgtttttgt gcaaacaata aaggcctgac aatattttct ctctttactt
     3241 caataccatg ccatgcagcc aaaccaccac cccgactcaa attaattaat acagtttctg
     3301 caacatcatc ggcatgatga gccgtagcaa taaaatcata atgaaagcta ctacataact
     3361 cctgaaaaaa attatatcta agttcacgtg ctgtaccctg aatggattgt tgattttgtt
     3421 ttgccaactt ttctgtttca aattcagtag aatgaatcac aattttgttt tccaaacaaa
     3481 atgaatgaac aaactgtgca tcaccatcag attcttcacc acgcaattta aaattacaat
     3541 gtgcaacagc aattttatat cctgactgta gcagtagttg caataaaacc attgaatcaa
     3601 tgcctccact aacagctaca agaattgttt gctctttact gaagagtttt ttctctttta
     3661 caaattgttg aaactgtaat aacaagtgtt gctttatttt ctgctaatgt acatcatcct
     3721 aaaacatcca tcattgcttt agcttttaac aaacactctt catactcggc agcagggtca
     3781 gattgaatgg ttattgctcc gcccgtctgc actgaaacat agttacgttg tgcattatac
     3841 aatatagtac ggataataac attgaagtca aaatcgcctg aaggatcaat ataaccaaca
     3901 ctcccggaaa acaagcctct gtttgttttc tcatgttcat caatcaatgt catagcgctt
     3961 attttaggag cacctgtcat acttcccata ggaaatgcag cacgtatgat ttcagaaaaa
     4021 ttaattttgg gttcaacctc acagcttact gtagaaatca attgatgtac tgttttaaac
     4081 gtatagacac taagcaactc attcacttca actgtgccgg gcttagcaat tcttgaaagg
     4141 tcatttcgta ccaaatcaac aatcatcaaa ttttctgcac gttctttttc actattctgc
     4201 agattatctt taagccattc atcttcctca cgtgatgcgc tgcgtggtgc tgttcccttt
     4261 attggctgcg aataaatttt atttcccctt ctacacataa accgctcagg acttgatgaa
     4321 ataataaatt gattttcttt tttataaaaa cacgacatgg gagcacgtgt gtttgtatta
     4381 agttttatat aggtttcaaa cggattgatg tttgctgcat cattataaaa ctcctgacaa
     4441 aaagttactt cataaatatt tccctgctgt aactgtctct ttaatttttc aacagcttgt
     4501 atgtaatcat cattgctgaa acgaggcttt aaagaaacat tagcagatgc attcatttgt
     4561 ctaagctcta catcatcggc aacaaattct ttcgatgtct gttcaagctg atcaatcata
     4621 cgaagttttc caccttcaga aactaaaaca acttgtggta caaaaaaatg taaaacagga
     4681 aaattcaatc ggtctgtatg cgatgatttt agttgttcga tttcattttt tagctcatac
     4741 gacataaaac caaacatcca cttgccttta ttcttctgaa taaatgtctc cagcttctct
     4801 aaagattctg atgttgcctc aactgtatcg agcgcattaa tgcctgcaag aaaatcatat
     4861 tttgtcaagc cactatagct accttctgca gcattgttgc tgtttaaaaa acaaaagtta
     4921 cgaaaaccat ttgatgactt taacagctct tcaaattcgg caggtgaaaa tttagtgtcg
     4981 ctcataaagc aaaaaaaatc aaaaaagatt tctgttgttt atgattctaa aaaagtagtg
     5041 aacacgtttt taacaagttc aataaattga aatgttttat aagtattatt caccgataat
     5101 taccctctgt caacacccat tttactctcg ggtgctccgg taattttatt tcttaatagt
     5161 gcaacatacg acatgacctt agagttcata agtagaatag ctacacagaa acaaaaaaca
     5221 aggaatgtga taaaccgcag tttacttaac ggaagaacca aaacaataaa taaaatgttg
     5281 aatgcagcca taataaaagt gcctccacga tgtccgaatt gctgacggca aatcatgtga
     5341 tgtaaatggc ttctgtctgc tttaaacggt gaaataccat ttgttaccct gataaaaaca
     5401 actcttgtta aatcaaatat tagtataaaa attgttgcaa tggttaacga agggccaaaa
     5461 gaaactacgg gagatgtaaa ccaaagatta aaaagtttta aagtgcaaat ggcaataaga
     5521 aaacctacaa ctaacgaacc cgtatcgccc ataaatactc ttgcaggatt aaaattaaaa
     5581 tacagaaaag ataccaaagc accaataaca ctgacacata aaactgcata agcctgttgt
     5641 ccatgaatat gaaacaatac agtaaaaatg ctgaatataa taagccctat tgttgctgcc
     5701 aagccatcta atccatcaat aaaattatag gcgtttacta aaaatattat aataaaaata
     5761 ctaatgccat attgagctat gataggtaat tcatgaatgc ctgtaaaacc atttaatgtt
     5821 tgaatgcgta atcctgaata ggtgactatt aaagctgcca agagttgtat atttaatttt
     5881 actcttgctt ttaaatccgt aagatcatca aaaaatccgg ttataaataa aatgagcatt
     5941 gccgctgcaa tggaatgaaa acttgaatcc tgattgttgt atccaaacaa aaagatcgaa
     6001 atcattatag caataaaaat tccgatacct cctaacgaag ggataattgc tgtatgatgt
     6061 ttgcgagtgt cggggcggtc aaacaaattc catttagaac aaagctgaat ccacgatgga
     6121 atgatgagtg atgcaataat aagagaagaa ataaatggaa ataacagttg aataatctga
     6181 ttcatacagg tggtttattt aatcaataat atatctgctc gcgatagtct ttctacttgg
     6241 attccgtctt ctatcattaa tgcaggcatt ttgtctttaa catcagcaat caggattctg
     6301 tcagcatcaa ttcctttacc tatcatataa tctttaaccg tttctgctct ttgttttcct
     6361 atactgttat ttttctttat ttcaatttcc gaagcatttc ctgaaacaat aatttttctg
     6421 tttgtgtttt tatacaaata atcaattgta gcttctatta catcaaagcg gttattgata
     6481 tcatacttca aactattatc ctgaaacact acatacccta ttgtttgata gttgtaatta
     6541 gaaagttctg ttttctcttt catactgctt ttacctttca ttggcatttc tgcaagtgaa
     6601 acaactatag gattctgttc aattattgaa ggagcaatta caggtatagg tgtcagcact
     6661 tgtggaggag taactctttg ctcaggcaat tttttcttat tgacaggctc aggttttctt
     6721 tcttcattac gtttaccttt tgcacgacct tgtggcgtgt aaacgagtga aatttcataa
     6781 gcgccactac ctcccggctt gtacttcgaa atattaaagt catagcttgt tccaagaaat
     6841 attttattaa actggtagcc cgcataagca accatagcat cgttatttct gtaaagcaat
     6901 cctaatttta ctaaactgtt attgcttaat gacaattcag aaatcatacc agca
//
LOCUS       DAORQT010000076         6909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1705_length_6909_cov_10.558944, whole genome shotgun sequence.
ACCESSION   DAORQT010000076 DAORQT010000000
VERSION     DAORQT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_6909_cov_10.558944"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2770
                     /locus_tag="PK134_04120"
     CDS             <1..2770
                     /locus_tag="PK134_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRB38064.1"
                     /translation="IDIYFARQLINEKLKEAEEKIPKGIGTPELAPVSTGLGEIYQYV
                     IHPKKGAENKYSAMDLRTMQDWIVDRQLYGTKGVAEVNSFGGLLKQYEVAVNPYRLKG
                     MNVTIAEIFSALEKNNENTGGAYIDKKPNAYFIRGVGLLGSMDDINNTVIKKVNDIPI
                     LIRDVAEVKLGNAVRYGSVTFNGEKEVVGGIVMMLKGANSAAVVERVKAKMEIIKKAI
                     PEDVEIEAYADRTSLVNRAVGTVKTNLIEGALIVLLVLILFLGNLRAGLIVASAIPLS
                     MLFALGMMNVFGVSANLMSLGAIDFGLIVDGAVIIVEATLHFLVVKHVAGKLTQKQMD
                     EAVEGSAKKMMSSAAFGQIIILIVYLPILSLQGIEGKMFRPMAQTVGFAILGALILSL
                     TYIPMMSALFLSKKVSHKKNIADKMMDLFQRGYAPLIRWAIKMKYAVVGGAVALLAVA
                     LFFFGRMGGEFIPQLQEGDYAFHCILPQGTSLTQSVETSMQASRIIKSFPEVEMVVGK
                     TGSAEVPTDPMPPEASDLIIKLKPKSQWTTTKDYTKLSEMMIEKLEVIPGVFFEASQP
                     IQMRFNELMTGVRQDVAIKIFGENIDTLAILAPQVARIVQSVKGATEPQVERTTGLPQ
                     ITIQYDRAKIAGYGLNIEDINHTISTAFAGEAAGVIFENERKFDLVVRLDSASRTSID
                     DVSNLFVATTDGNQIPLSQVATVSFKEGPAQISREEGKRRIVVGFNVKDRDVASVVKE
                     IQEKLNVAKILPTGYYYTYGGTFENLQEASARLKIAVPVALALIFLLLYFTFGSMKEA
                     ALIFTAIPMSAIGGVFALLLRGMPFSISAGVGFIALFGVAVLNGIVLISTFNQLEKDG
                     IKDIIQRVIEGTKIRLRPVLMTAMVASLGFIPMALSSGAGAEVQKPLATVVIGGLITA
                     FALHYFFI"
     gene            2784..3995
                     /locus_tag="PK134_04125"
     CDS             2784..3995
                     /locus_tag="PK134_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB38065.1"
                     /translation="MKPVTTVIAGIVLLCLSNNVQAQQPSAKRVSITEAITLAKNNLQ
                     YEINNQQISKGQAQKKTATALPKTGVFAENEDFRPSDNKGILKIGLSQSVAWPGLYKA
                     QKNLYGEQLKYYQANTAAIEIDIKRDVRTVYYQLWYLQDKQQLYHRLDSIYKSLSNAA
                     ILKVKTGDSPGLDSIAANVRMSELQALLIQMSNEIQIQQQSLMQLVNTNELMLPVMMP
                     LEKLSMPGIANDSIHPVLALQSQTVNIANAGIGVIKNENKPEFAGRFFSQRLWGAKDP
                     FTGFSVSAAFPLFGANAYRNKVKVAQADMAVQQKQYDYDKQLLTTRQLQMQQEVGKNK
                     SMLSFYETTGLRQADEIIKAASLAYRAGEISFAELSQFLTQAIEIQKNYLENLNEYNQ
                     SVIQYYYYINQ"
     gene            4007..5263
                     /locus_tag="PK134_04130"
     CDS             4007..5263
                     /locus_tag="PK134_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB38066.1"
                     /translation="MKKFLIIAIILTSLVSVTSCGSKGNAEAAKPEEEHTDEHENKNT
                     ATLTEEQIKSIGVELGVIEDKQLTAALKTNGVLKVPNQNKASVNSIYSGVIKSLSVQP
                     GNVVRKGQVIATVANPEFIQVQSDYLSVNGKIVMAELEVKRQKELNAGNAGALKNLQA
                     AETELRTLRTLKSTLQQQIQLMGINPARLSSGKLISVLAITSPISGVVSNVMVKMGSY
                     VDVSTAVAEIVDNSQLHLDLFVYEKDLPKLKNNQLIHFTLTNNPGKEYDAQIFSLGSS
                     FEGESKAVTVHAMVQGDKTGLIDGMNITAAISLEKATVPAVPTEAIITIAGQDYIFML
                     TDKHAEDEHTAGSKDSIKHNVKEEPEHEEEKGLTFEIIPVAKGTTDVGYTEITLLKEI
                     PKGAKIVVKGAFFVLAKMTNTGEHEH"
     gene            5275..5646
                     /locus_tag="PK134_04135"
     CDS             5275..5646
                     /locus_tag="PK134_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HRB38067.1"
                     /translation="MKFYTAMPKTLKPFYQTELNKATTLLQQGKLQECWRHYERAHIL
                     GQRYPLQHSYVHWLMLKFGIKIKSTKEVLGQITRLVFGGVKSFVGTVPIGNTGGANVP
                     PLKQMEIPEDLAAIINNTKTN"
     gene            5846..>6909
                     /locus_tag="PK134_04140"
     CDS             5846..>6909
                     /locus_tag="PK134_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRB38068.1"
                     /translation="MINKDPKHKHTYDAQGRMTCCTQEEKIYSKAGASSLLKEGHSHD
                     DGHDHDHEEGDGHDHSGGSQTTLQMFLPAIISLILLFAAIALDNWFPQSWFTGWVRIV
                     WYAIAYLPVGFPVIKEAVESIGKGEIFSEFLLMTIATIGAFAIGEYPEGVAVMLFYAV
                     GEVFQTMAVKRAKANIKSLLDQRPDEVTILENNQAKTIKAADAKIGDIIQLKSGEKLG
                     LDGELISETASFNTAALTGESKPDTKAKGETVLAGMINLNTVAQIKVTTAYTDSKLSK
                     ILEMVQNATAQKAPTELFIRKFAKIYTPIVVALAILICVLPMLFVDNYVFRDWLYRAL
                     VFLVISCPCALVISIPLGYFG"
BASE COUNT         2364 a         1177 c         1486 g         1882 t
ORIGIN      
        1 tattgatatt tattttgccc gtcagttaat caatgaaaaa ctgaaagaag ccgaagaaaa
       61 aattccaaaa ggtattggta ctcccgaatt ggccccggtt agtaccggtt taggagaaat
      121 atatcaatat gtaattcatc cgaaaaaagg ggcagaaaat aaatattctg caatggactt
      181 gcgtactatg caggactgga ttgtagaccg tcagttgtat ggcacaaaag gcgttgcgga
      241 agtaaacagc tttggcggtt tattaaagca atatgaagta gccgttaatc cttaccgtct
      301 taaagggatg aatgtaacca ttgctgaaat attttctgct ttggaaaaga ataatgaaaa
      361 tactggtggt gcatacattg ataaaaaacc caatgcctat ttcatccgtg gtgtcggctt
      421 attaggttca atggatgata taaataatac tgtaattaag aaagtaaatg atattccgat
      481 attaataagg gatgtagctg aagtaaaatt gggcaatgca gttcgttacg gttcagtaac
      541 ctttaacggg gaaaaagaag tagttggtgg tattgtaatg atgctgaaag gtgctaacag
      601 tgcagccgta gtagaaaggg taaaagcaaa aatggaaatc attaaaaaag caatacctga
      661 agatgtggaa atagaagctt atgcagacag aacaagtttg gtaaaccgtg ccgtagggac
      721 agttaaaaca aatcttattg aaggagcttt aatagtatta cttgtactga tattgttttt
      781 aggtaatctc cgggcaggtt taatcgtagc atcggctatt ccgctttcta tgctgtttgc
      841 tttgggtatg atgaatgtgt ttggcgtaag tgccaacctg atgagtttag gtgcaattga
      901 ttttggattg attgtagatg gagctgtaat tattgtggaa gcaacactgc attttttggt
      961 agtaaagcat gtggccggga agcttacgca aaaacaaatg gatgaagccg tagagggcag
     1021 cgcaaaaaaa atgatgagtt cagcagcctt cggacaaatt attattttaa ttgtgtacct
     1081 gcctatactt tccttacagg gtattgaagg aaaaatgttc cggccaatgg ctcaaacggt
     1141 tgggtttgcc atattaggag cattgatact ttcacttact tatattccga tgatgtcggc
     1201 tttatttctt tccaaaaaag tatcgcacaa gaaaaatatt gctgataaaa tgatggattt
     1261 atttcaacgg ggctatgcac cgttgatacg ttgggctatt aaaatgaaat atgcagtagt
     1321 gggcggtgct gttgcactct tagctgttgc tctatttttc tttggcagaa tgggtggcga
     1381 atttattccg cagttacagg aaggtgatta tgcttttcac tgcatattgc cacagggaac
     1441 ttcattaacg caaagtgtgg aaacatccat gcaggcaagc cgcatcatca aatcatttcc
     1501 tgaagtggaa atggtggtag gcaaaacagg aagtgcagaa gtaccaaccg accctatgcc
     1561 acccgaagca tccgacctca tcattaaact gaaaccaaaa agccaatgga caaccacaaa
     1621 ggattatact aaactgtctg aaatgatgat tgaaaaatta gaagtaatcc caggtgtttt
     1681 ctttgaagca tcgcagccaa ttcaaatgag gtttaatgaa ctgatgacag gtgtgcggca
     1741 ggatgtagca attaaaatat tcggagaaaa tattgatacc ctggctatac ttgccccaca
     1801 agttgccaga atagtgcaat ccgtaaaagg tgcaacagaa ccacaggtag aaagaaccac
     1861 tggcttacca caaatcacta ttcaatacga cagggcaaaa attgcaggtt acggattgaa
     1921 tattgaagac ataaaccata ctatcagtac agcctttgca ggtgaagcag cgggagtgat
     1981 ttttgaaaat gaacgaaagt ttgatttagt ggtaaggctc gatagtgcaa gccgtacatc
     2041 tattgatgat gtcagcaatt tatttgtggc aactacagac gggaatcaga ttccattatc
     2101 acaggtagca accgtttctt ttaaagaagg tcctgcacaa attagccgtg aagaaggtaa
     2161 acgtagaata gtggttggct ttaatgtgaa agaccgtgat gtagccagtg tggttaagga
     2221 gattcaggaa aaactaaatg ttgccaaaat tctgccaaca ggatattact atacttacgg
     2281 cggcacattt gaaaatttac aggaagcttc tgcaagatta aaaattgcag tgccggtggc
     2341 actggcattg attttccttt tactctactt caccttcggt tcaatgaaag aagctgcact
     2401 tatttttact gctatcccaa tgagtgcaat aggtggtgtg tttgctctgc tgcttagagg
     2461 aatgccattc agtatttccg caggtgtggg ctttattgca ttgtttggag tagcagtgtt
     2521 aaatggtatt gttttaatca gcacctttaa tcagttagaa aaagacggta ttaaagacat
     2581 tatacaaaga gtaatagaag gcacaaaaat ccgtctgcgg ccagtgttaa tgactgctat
     2641 ggtagcatca ttaggtttta taccaatggc attatcaagt ggcgcaggtg cagaagtgca
     2701 aaagccattg gcaacagttg taattggcgg tttgataaca gcctttgctc tacattattt
     2761 tttcatctaa aagaaaaccc aaaatgaaac ctgtaacaac agtaatagca ggtattgttt
     2821 tattgtgttt gtcaaacaat gtgcaggcac agcaaccatc tgcaaaaaga gtaagtatta
     2881 cagaagctat tactttggca aaaaataacc tgcaatacga aataaataac cagcagataa
     2941 gtaaaggtca ggcacaaaaa aaaacagcaa ctgctttacc aaagacaggt gtatttgcag
     3001 aaaatgaaga ttttcggcca agcgataata aaggcatact taaaattggc ctatcacaaa
     3061 gcgtagcatg gccaggttta tacaaagcac aaaaaaattt gtatggcgaa cagttgaagt
     3121 attaccaggc aaatacagcc gctattgaaa ttgatattaa aagagatgta agaacagttt
     3181 attatcagct atggtacttg caggacaaac agcaattgta tcaccgctta gatagcattt
     3241 acaaatcatt aagcaatgca gccatcctta aagtaaaaac tggcgacagt ccgggattag
     3301 acagcattgc agccaatgta agaatgagtg agctacaggc attgttaatt caaatgagta
     3361 atgaaattca gatacaacag cagtcgttga tgcaattagt taatacaaac gaactgatgc
     3421 tgccggtaat gatgccactg gaaaaattat caatgccggg aattgcaaat gatagtattc
     3481 accccgtact ggcattgcaa tcgcaaacag tcaatattgc caatgcagga attggagtaa
     3541 taaagaatga aaataaaccc gagtttgcag gccgtttctt tagtcagcga ttgtgggggg
     3601 caaaagaccc gtttactggc ttttctgtat cagcagcctt tccgttgttt ggtgcaaatg
     3661 cttaccgcaa taaagtaaaa gtggctcagg ctgatatggc tgtgcagcaa aagcaatacg
     3721 actatgataa acagctttta actacccggc aattgcaaat gcagcaggaa gtaggtaaga
     3781 acaaaagcat gttgtctttt tatgaaacaa ccggtttgcg gcaggctgat gaaataatta
     3841 aagcagcatc attggcttac cgtgccggtg aaataagttt tgctgaacta tcacaattct
     3901 taacacaagc cattgagatt caaaaaaact atttagaaaa tctgaatgaa tacaatcagt
     3961 cagtgattca gtattactat tacatcaatc aataaaaaat ataacgatga agaagttttt
     4021 aataatagca atcattctca caagtcttgt atccgttact tcctgcggta gtaaaggcaa
     4081 tgccgaagca gccaaacccg aagaagagca tacagacgaa catgaaaaca aaaacacagc
     4141 aactctgaca gaggaacaga ttaaaagcat tggcgttgaa ttaggagtta ttgaagacaa
     4201 acaactaaca gcggcactca aaactaatgg tgtgctgaaa gtacctaatc aaaacaaagc
     4261 cagtgtaaac tctatttaca gcggagtaat aaaatcctta tccgtacaac ctggtaatgt
     4321 tgtaagaaaa ggacaggtga tagccaccgt tgccaaccct gaatttatac aagtgcaaag
     4381 tgattacctg agtgtgaatg gaaaaattgt aatggcagaa ttagaagtaa aacggcaaaa
     4441 agaactgaat gcaggaaatg caggagcttt aaaaaattta caggcagcag agacagaatt
     4501 aagaacattg cggacactaa aatcaacatt gcagcagcaa atacaattga tgggtattaa
     4561 tccggcaagg ctttccagtg gcaagttgat ttctgtactt gctatcacaa gccctatcag
     4621 tggtgtagta agtaatgtaa tggtaaaaat gggcagttat gtagatgtaa gtactgccgt
     4681 tgctgaaatt gttgacaaca gccaattgca ccttgatttg tttgtatatg aaaaagattt
     4741 gcccaagctg aaaaataacc agctaataca tttcaccctt accaacaatc cgggtaaaga
     4801 atatgatgca cagatttttt ctttaggctc atcttttgaa ggggagagca aagcagttac
     4861 ggtacatgct atggtgcagg gtgataaaac aggattaata gacggtatga atattactgc
     4921 cgctatcagt ttggaaaaag caaccgtacc ggctgtgcca acagaagcca taataactat
     4981 agccggacaa gattatatct ttatgcttac agataaacat gctgaagatg aacatactgc
     5041 cggtagtaaa gacagtatta aacataatgt taaggaggaa ccggaacatg aggaagaaaa
     5101 aggactgacc tttgaaatca tacctgttgc caaaggcact actgatgttg gctacacaga
     5161 aataacctta cttaaagaaa ttcccaaagg tgcaaagatt gttgtgaaag gtgctttttt
     5221 tgtattagca aaaatgacca ataccggcga acatgaacat taaaaaaaag cagcatgaaa
     5281 ttttatacag caatgccaaa aacactaaaa cccttttatc aaactgaact gaataaagca
     5341 acaactttat tacagcaggg aaaattacaa gagtgctggc ggcattatga aagggctcac
     5401 attttagggc aacggtatcc actgcaacat tcctatgtac attggctaat gttgaagttt
     5461 ggtattaaaa taaaaagcac aaaagaagtt ttaggccaaa taacccgttt ggtttttggc
     5521 ggtgtaaaat catttgttgg tactgtgcca attggtaaca ccggtggtgc taatgtgccc
     5581 ccgttaaaac aaatggaaat accggaagac cttgcggcaa tcattaacaa tactaaaact
     5641 aattagtatg tatttttaca ttcataaaat ttataaaaat gaaaatgaac aggctcagtt
     5701 gctggaaatg tgtggtgatg cttgcatccg tcttaggaat ggtaagtgct ttcagggctt
     5761 aatactaaca acacatttaa tataaagggt tgcagatgga aacgtctgca accttttttt
     5821 atttgcaata tttaaaaggt aatacatgat aaataaagac ccaaaacata aacacaccta
     5881 cgatgcacaa ggcaggatga cttgctgtac acaggaagaa aaaatttaca gcaaagccgg
     5941 tgcttcatca ttattgaaag aaggccatag ccatgatgat ggacatgacc acgaccatga
     6001 agaaggcgac gggcatgacc attcaggagg cagtcaaaca acattgcaaa tgtttctacc
     6061 tgcaatcatt agtttgattc ttttatttgc tgccattgca ttagataatt ggtttccgca
     6121 aagctggttt acaggatggg taaggattgt ttggtatgca atagcttatt tacccgtagg
     6181 ctttcctgta attaaagaag cagttgaaag cattggaaaa ggcgaaatat tttcagagtt
     6241 tttattaatg actattgcaa ccattggtgc atttgccatt ggcgaatatc ctgaaggcgt
     6301 agcagtaatg ttattctatg cagttggtga agtgtttcag acgatggcag tgaaaagagc
     6361 aaaggcaaat ataaaatcct tgcttgacca gcgacctgat gaagtgacga ttttagaaaa
     6421 taaccaggct aaaaccataa aagcggcaga tgcaaaaatt ggcgatatca tacaattaaa
     6481 gtcaggtgaa aaattaggat tggatgggga gttaatttca gaaactgcat cattcaatac
     6541 cgcagcactc accggcgaaa gcaaaccaga cactaaagca aaaggtgaaa ctgttttggc
     6601 tggcatgata aatttaaaca ccgttgcaca gataaaagtt actactgctt acacagatag
     6661 taagttgagc aagattttag aaatggtgca aaatgctact gctcaaaaag cacctactga
     6721 attattcatc cgcaagtttg caaaaatcta tacaccgatt gttgttgctt tagccattct
     6781 gatttgtgtt ttaccaatgc tatttgtaga taattatgtt ttcagagatt ggttatacag
     6841 ggcattggtt ttcttagtta tctcatgccc ctgtgcattg gttatttcaa ttccgttagg
     6901 ctattttgg
//
LOCUS       DAORQT010000077         6897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1712_length_6897_cov_12.351798, whole genome shotgun sequence.
ACCESSION   DAORQT010000077 DAORQT010000000
VERSION     DAORQT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_6897_cov_12.351798"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1546
                     /locus_tag="PK134_04145"
     CDS             248..1546
                     /locus_tag="PK134_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38069.1"
                     /translation="MRKIFISILFIISAGITNAQIDNLSNMSPEWIRTGGRNAAIKGS
                     DLVVYNPATLTRLEKGLHIGLGNQMFMRKPSHTYDLGWGEVKHEQDGNDYFVPNLFLS
                     YNKNKWAVWGGVYISGGGATANYPEGSLNTELIGLSLLSAFQGAYLGTGQQYLKASSL
                     YLTGTAGFSYAINEQFSAALGVRYVNATNKTKAGTELIGDFSNLPVNMEQEDNANGIA
                     AVISLSARPIEKLNIAVRLETNTKLNFKTKQKVDDLEQSTGGMAELVVDGQESRRDLP
                     GLAGIGIGYEFSKAFRLTAEMNYYFQQHADWGKRADGKRINTLAGDAMNFGGGFEWMW
                     SKYITWSAGAVYTMLDFNDMAGYYTTLGAFETAPYNNLTANTGFAIDLSEKFRVNVGV
                     AKAFYKKDKTVAIEAMAPNVVKAKLNNDVLMLGAGIDLRF"
     gene            complement(1555..2307)
                     /locus_tag="PK134_04150"
     CDS             complement(1555..2307)
                     /locus_tag="PK134_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB38070.1"
                     /translation="MIAIENISKSFGNKKVLEDVSCEFDKGKINLIIGGSGQGKTVLM
                     KCAVGLFEVDKGKVLYDGRNFSEMTFGERKPIRQEIGMLFQGGALFDSLTVEQNVTFP
                     LKMFTNMTNAEKRDRANFCLHRVNLENVNHLLPSAISGGMKKRVAIARAIAMNPRYLF
                     CDEPNSGLDPKTSILIDELIKEITEEYDITTVINTHDMNTVMSMGEKIIFIYKGKKFW
                     EGNNEDIMRSENKELNDFVFASRWMQAVKKHF"
     gene            complement(2304..3047)
                     /locus_tag="PK134_04155"
     CDS             complement(2304..3047)
                     /locus_tag="PK134_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB38071.1"
                     /translation="MKGTLFEIGRYFGFIKNLFYKPEKLSIYVRQVFREMVNLGIGSL
                     WLVALTSAFMGAVITIQSAYNLVNPMVPLWAVGIVARDSIIIEFSPTIIMLVLAGKVG
                     SSVASEIGTMRVTEQIDALEIMGINSSGYLVLPKIIAALLICPVVVIISMIVGITGGW
                     VAGDLSGVVPSADYLKGIQNQFLPFNVTFAMIKTVVFAFIITSVPAYHGYYTSGGALE
                     VGQSSTKGVVYSSILILIFDYLLTQLILA"
     gene            complement(3072..4118)
                     /locus_tag="PK134_04160"
     CDS             complement(3072..4118)
                     /locus_tag="PK134_04160"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRB38072.1"
                     /translation="MLKKILITAWIISMATGLQAQQKKSYQVGVIAFYNLENLFDTIN
                     QPEVNDEEFTPTGSNNYTGKVYLDKLGKLSDVLSLIGKDESPDGFSVLGCAEVENESV
                     LKDLVNEPKLKSRNLKIVHYDSPDKRGVDVALLYNPKYFTPKFSEPLFIDLRTPENPE
                     YYTRDILYVYGLYLGEPVHFLVGHWPSRRGGEEASAPSRAKAAAVCKNKIDSITKINA
                     DAKIVLMGDLNDDPLSPSVTKVIKAVGSKDKVERGGMYNPWVDFYTKGIGTLAYNDAW
                     NLFDQIMISSGWLNKKQDGFFFKDAHIFKREFMLQKSGRYKGYPLRTFDFSRYIGGYS
                     DHFPTYLVFLKEVK"
     gene            complement(4190..6484)
                     /gene="rnr"
                     /locus_tag="PK134_04165"
     CDS             complement(4190..6484)
                     /gene="rnr"
                     /locus_tag="PK134_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRB38073.1"
                     /translation="MSKKNIKFTKTQLEKLKQELLQVFLQHPRATFNPKQVWRKAEIN
                     LPEEYISVVLNADKHYIDETIQSLILELFQEGELLEVQQFRYKLLPAERFIEGNIQVT
                     SKGEGYVMNEGFEEDIQIPAHLMANALNGDKVRISLLAHREGRRQQGEVVEVLSRSRT
                     QFAGTIQHSGSYAFVVCDDPKMHVDIFIPGKQLNGARNGDKVIAEITLWSADASNPEG
                     KIVRVLGAPGEHTAEMNAIIVEYGLPEAFPDEVEKETLKISDVISKEEIKKRRDFRKV
                     TTFTIDPVDAKDFDDALSIQQLPNGNWEMGVHIADVSHFLKLGSAMDEEAFNRATSIY
                     LVDRVIPMLPEKLSNMVCSLRPNEEKLCYSAVFEMNEKAEVLNEWFGRTVIFSDHRFT
                     YEEAQQVIETNEGPFVKEIGVFHRLAQQLRKERHRKGAISFEKTEVKFRLDEKGNPIG
                     IFLKESKDSNKLIEEFMLLANRRVAEFIGKGVAKGKKDKTQKTFVYRIHDVPVEEKLS
                     DFKTFAAQFGYRISTASGKETAHSLNKMLKDVEGKKEQSMIEQLAIRSMAKAVYSTEN
                     IGHYGLAFDFYTHFTSPIRRYPDVMVHRLLDYYMSGGKSVSQQEYEEKCRHCTEREIN
                     AADAERSSIKYKQVQYLAARKDEIFKGVISGVTEWGLYVELEDNKCEGMIRLRDLNDD
                     YYEFDQKNFCIVGHRSRKKYSLGDELMVVIKSTDIIKKQVDFMLYDANRLHEVSPFKR
                     SDKKRFPTSGKFRGDKKKGGKRRR"
     gene            complement(6581..6880)
                     /locus_tag="PK134_04170"
     CDS             complement(6581..6880)
                     /locus_tag="PK134_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRB38074.1"
                     /translation="MNTVHEITTSNTNVLIDFYADWCEPCKWLNPVLEELTQQLPDLY
                     IHKINIDQFTEEAAAFEIKSVPVLILFVDGKQVWRMNGFKYAKELAKELKEFLKA"
BASE COUNT         2079 a         1399 c         1246 g         2173 t
ORIGIN      
        1 ttgtgggcaa gtgaagccaa acacggaaat atttttgtaa aaatggcgct tgaatattat
       61 ccgaaagatg atgtatataa acgtctgcat gaactcactg taaaagaggg tgaaatatgt
      121 aaagcactac tcataaaagc agcattgcat taatcactta ttaaaaacaa attgtttatt
      181 agtttctttt ctgcatggaa tgacaatgca aatgcaaaat atttttactc aaacaaaaaa
      241 tcagagtatg aggaaaatat ttatcagcat tctatttatt attagtgcag gaataacaaa
      301 tgcacagatt gataatttga gcaacatgag cccggagtgg attcgtacag gaggcagaaa
      361 tgctgcaatc aaaggcagtg atcttgttgt ttataatccc gctacattaa cacgtttgga
      421 aaaaggacta catattggtt tgggcaatca gatgtttatg cgcaaacctt ctcacaccta
      481 tgatttaggt tggggtgaag taaaacacga acaggatggt aatgattatt ttgtacctaa
      541 cttattttta tcatacaaca aaaataaatg ggctgtgtgg ggtggtgtat atatttccgg
      601 tggaggtgcc actgccaatt atcctgaagg ctctttaaac acagaactta ttggactgtc
      661 acttctcagt gcttttcaag gtgcctacct tggaacagga cagcaatatc ttaaagcatc
      721 ttctctttac cttactggaa cagcagggtt cagttacgct ataaacgagc agttttctgc
      781 agcattgggt gtacgctatg taaatgccac caacaaaact aaagcaggca cagagttgat
      841 aggcgatttc agtaacctgc ccgtaaatat ggagcaagaa gataacgcta acggtattgc
      901 cgcagttatc agcttaagtg cgcgacccat agagaaatta aatattgctg taagactgga
      961 aacaaacacc aagctaaatt ttaaaaccaa acaaaaagtt gatgaccttg aacagtcaac
     1021 cggtggcatg gcagagctgg tagttgacgg acaagaaagc agacgcgatt taccgggttt
     1081 ggctggtatt ggtataggat acgaattcag taaagcattt cgtctgacag cagaaatgaa
     1141 ttactacttt cagcaacatg ccgactgggg aaaacgtgcc gatggaaaaa gaataaacac
     1201 acttgcaggc gatgctatga attttggtgg tggttttgag tggatgtggt caaagtatat
     1261 tacctggagt gcaggtgcag tttataccat gctcgatttt aatgatatgg caggttatta
     1321 cactacatta ggagcttttg aaacagcacc ctacaacaac cttactgcaa acacaggttt
     1381 tgcaattgac ttatcagaaa aattcagagt gaacgtaggt gtggcaaagg ccttttataa
     1441 gaaagataaa acggtagcta ttgaagctat ggcacctaat gtggttaaag caaaactaaa
     1501 caatgatgtg ttgatgcttg gtgcaggtat tgatttgaga ttctgacgga tttattaaaa
     1561 gtgcttttta acagcctgca tccatcgtga tgcaaaaaca aaatcgttta gttctttatt
     1621 ttcagagcgc atgatgtctt cgttgttgcc ttcccagaat tttttgcctt tatagataaa
     1681 aataattttc tctcccatac tcatcactgt attcatgtca tgtgtgttga tgacagtagt
     1741 aatgtcatat tcctccgtaa tttcttttat gagttcgtca atgagaatgg atgttttagg
     1801 gtcgaggcca gagtttggtt catcgcaaaa taaatatctt ggattcattg caatggcgcg
     1861 tgcaatggct acacgttttt tcatgccacc actgatggca cttggcaaaa gatgattaac
     1921 gttttcgagg tttactctat gcagacaaaa gttggcacga tcgcgctttt ctgcattggt
     1981 catgttagta aacattttca aagggaaagt tacattttgt tcaacggtta atgaatcaaa
     2041 caatgcacca ccctgaaaca acatgccaat ttcctgacga atgggtttac gttcgccaaa
     2101 agtcatctcc gaaaaatttc tgccatcgta taaaactttc cccttatcaa cttcaaataa
     2161 accaacagca catttcatca gcacagtttt tccttgtcca ctaccgccaa taatcaggtt
     2221 aattttacct ttgtcaaatt cgcatgaaac atcttcaagc acttttttat tgccgaatga
     2281 tttggatatg ttttcaatgg caatcatgca agaatgagtt gggttaataa gtaatcaaaa
     2341 atcagaatta aaatactgct gtaaactaca cctttggtac ttgactggcc aacttcaagt
     2401 gcgccaccgc ttgtataata gccatgatag gcaggaacag aagtgataat aaaagcaaac
     2461 actactgtct ttatcatagc aaaggttaca ttgaatggta agaactggtt ttgtattcct
     2521 tttaaataat ctgccgaagg cacaacgccc gaaagatcac ctgcaaccca tccgcctgta
     2581 ataccaacaa tcatactgat gattacaact acgggacaaa ttaataatgc tgcaataatt
     2641 ttaggaagca ccaaataccc tgatgagttt atgcccatta tctcaagtgc atctatctgt
     2701 tcggtaacac gcatggtgcc aatttctgat gcaacactgc tacctacttt tcctgcaagt
     2761 acaagcatta tgatagttgg agaaaactca atgatgatac tatcgcgggc aacaatacct
     2821 actgcccaca aaggcaccat tgggttcaca aggttgtaag cactttgaat tgttataaca
     2881 gcacccatga aagcagaagt aagtgccacc agccacaatg aaccaatgcc taagtttacc
     2941 atttcgcgaa aaacctggcg tacataaatt gacaactttt caggtttata aaaaagattt
     3001 tttataaaac cgaaatagcg tccaatctca aataatgtac ctttcatcag tagctaagat
     3061 attaaaacta tctattttac ttcttttaaa aatacgagat aggttggaaa gtgatcgctg
     3121 tagccgccaa tatatctgct gaagtcgaat gtgcgtaagg gatagccttt ataacgtccg
     3181 ctcttttgca acataaattc gcgtttaaaa atatgggcat ctttaaaaaa gaatccatct
     3241 tgctttttgt ttaaccagcc tgatgaaatc ataatctgat caaaaagatt ccatgcatcg
     3301 ttataggcaa gtgtacctat tccttttgta taaaaatcaa cccatggatt gtacatgcct
     3361 ccgcgttcta ctttgtcttt actgcccact gccttaatta cttttgtgac acttggactt
     3421 aaagggtcat catttaaatc gcccatcagc acaatctttg catcggcatt tattttagtt
     3481 atagaatcaa ttttgttttt acaaacagcg gcagccttag cgcgtgatgg tgcagaagcc
     3541 tcttcgcccc ctctgcgcga tggccagtga ccaacaagaa aatgtacagg ctctccaaga
     3601 tacaaaccat aaacatataa tatgtcgcgg gtgtagtatt cggggttttc aggagtacgc
     3661 aagtcaatga ataaaggctc actaaactta ggagtaaaat attttggatt atagagcaat
     3721 gctacatcaa caccgcgttt gtcagggctg tcgtaatgaa caattttcag attgcgtgat
     3781 tttagtttgg gttcattaac caaatctttt aaaacacttt cattctctac ttctgcacat
     3841 cccaaaacag aaaatccatc tggtgactcg tcttttccaa tcagtgataa tacatcagag
     3901 agtttaccta acttatcaag atatactttg cctgtgtaat tgttagaacc tgtcggagta
     3961 aactcttcat cattaacttc gggttgattg atggtatcga acaggttttc taaattgtag
     4021 aaagcaataa cacctacctg atagcttttc ttttgttgtg cctgaaggcc tgtagccatg
     4081 gaaattatcc aggctgtaat aagtatcttt tttaacatgg cacaaatcta aaaaacgtca
     4141 caatgctgtg gcttgaaatt atgatgatta aatttaattt ttaaaaagat tatcttcttc
     4201 ttttgccacc cttcttttta tcgcccctga acttgcctga tgtagggaat ctttttttat
     4261 cacttcgttt aaaaggagaa acttcatgca aacgatttgc atcataaagc ataaaatcaa
     4321 cttgtttttt aatgatgtcg gtactcttaa tgacaaccat cagctcatcg cccaaagaat
     4381 attttttgcg ggaacgatgt cctacaatgc aaaagttttt ctgatcaaat tcataatagt
     4441 catcattcag gtcgcgcagc cttatcatac cttcacactt attatcctcc aactccacat
     4501 aaagacccca ttccgtaaca ccggaaatta cacctttaaa tatttcatct ttacgtgctg
     4561 caaggtattg aacctgtttg tattttattg aagagcgctc tgcatcggca gcattaattt
     4621 cacgctccgt acagtgacgg catttttctt catactcttg ttgcgaaaca ctctttccac
     4681 cactcatata ataatccagc aatcggtgca ccatcacatc aggatagcgc cttatgggcg
     4741 aagtaaagtg tgtatagaaa tcgaatgcca atccatagtg acctatattt tcggtagaat
     4801 aaacagcctt agccatactg cgtatagcca attgctcaat catactctgc tcttttttac
     4861 cttccacatc tttcagcatt ttattgagtg aatgagctgt ttcttttcct gatgcggtgc
     4921 taatcctata gccaaactgt gctgcaaaag ttttaaagtc gctaagtttt tcttcaacag
     4981 gcacatcgtg gatacgataa acaaaagttt tttgtgtttt atctttcttc ccttttgcaa
     5041 cacctttgcc aataaactct gccacacgtc tgttggcaag taacataaac tcttcaatca
     5101 gtttattgga gtctttgctc tcctttagaa atattccaat tggatttccc ttttcatcaa
     5161 gcctgaattt tacttctgtt ttttcaaaac tgattgctcc cttacggtga cgctctttgc
     5221 gaagttgctg tgccagccgg tgaaacacac caatctcttt tacaaaaggg ccttcgttgg
     5281 tttcgattac ctgctgtgct tcttcataag taaagcgatg atctgaaaaa attacagttc
     5341 tgccgaacca ttcattcagg acttctgctt tttcattcat ctcaaaaacg gcagagtagc
     5401 aaagtttttc ctcattaggt cttagagagc aaaccatatt cgacagtttt tccggcaaca
     5461 taggaataac tctgtcaacc aaataaattg aagttgctct gttgaaggct tcttcatcca
     5521 tggcactacc cagttttaag aaatgtgaaa catcggcaat gtgtacaccc atttcccagt
     5581 tgccattagg caactgctga atgcttaatg catcatcaaa atcttttgca tcaaccgggt
     5641 caatagtaaa ggtggttact tttctgaagt ctctgcgttt tttaatttct tctttcgaaa
     5701 tgacatcaga aatttttaat gtttcttttt caacttcatc aggaaaggct tccggaagac
     5761 catactcaac aattatggca ttcatctctg cagtatgttc accgggagca cctaaaacac
     5821 gcactatttt tccttcggga ttactggcat cggcagacca taaggttatc tctgcaatta
     5881 ctttatcgcc attgcgggca ccattaagtt gttttccggg aataaaaata tctacatgca
     5941 ttttaggatc atcgcaaact acaaatgcat aactgcctga atgttgtatt gtgccggcaa
     6001 attgtgttct gcttcgactg agaacttcaa ccacttctcc ttgttgtctt ctgccttcgc
     6061 gatgcgctaa taaacttatt ctgactttgt cgccattcaa tgcgtttgcc attaaatgtg
     6121 caggaatttg tatatcctct tcaaaccctt cattcatcac atatccttcg cctttggaag
     6181 ttacctgaat gtttccttca ataaaccttt ctgcaggtaa taacttataa cgaaactgct
     6241 gaacctccag caactctcct tcttgaaaca gctctaaaat taaagattga attgtttcgt
     6301 ctatataatg tttatcagca ttcagcacta ctgaaatata ttcttcgggc aaatttattt
     6361 ctgctttgcg ccaaacctgt ttgggattaa aagtagcacg cgggtgttgc agaaaaacct
     6421 gtagcaattc ttgttttaat ttttcaagtt gtgtttttgt gaattttata ttcttctttg
     6481 acatgttttc gtttgattgt aaaattacgt gtagtgaaac agatgttagt catgctacac
     6541 attaaaaaat aaatgttttc aacagctata aagtaaggtc tcaggctttt aagaactcct
     6601 ttaactcttt ggctaattct ttcgcatact taaacccgtt catacgccaa acctgtttcc
     6661 catcaacaaa aagtatcagc acaggcacac tctttatttc aaatgctgca gcctcttcag
     6721 taaattggtc aatattgatt ttatggatat acaaatcggg aagttgttgc gtcaactcct
     6781 ccaaaaccgg attgagccac ttacagggtt cgcaccaatc ggcataaaaa tctatcagca
     6841 cattggtatt ggatgttgtt atttcatgga ctgtgttcac atcacaggtt gtgaagt
//
LOCUS       DAORQT010000078         6784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1752_length_6784_cov_10.227671, whole genome shotgun sequence.
ACCESSION   DAORQT010000078 DAORQT010000000
VERSION     DAORQT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_6784_cov_10.227671"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="radC"
                     /locus_tag="PK134_04175"
     CDS             <1..590
                     /gene="radC"
                     /locus_tag="PK134_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRB38075.1"
                     /translation="AILIRSGTRQESAVDLAKRILKNTHNELSELSKLSVKDMMKYKG
                     MGKVKSITIVAALELGRRRAQAQALERQKITSSKDAVAYFKALLADLPHEEFRILLLD
                     RANQIIKSENISKGGVSGTVVDARIIFKSAIENLASGIILCHNHPSGNPKPSQADVNL
                     TSKLSQAGKLLEINILDHVIIAGDDYYSFADEGQL"
     gene            663..1787
                     /gene="carA"
                     /locus_tag="PK134_04180"
     CDS             663..1787
                     /gene="carA"
                     /locus_tag="PK134_04180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRB38076.1"
                     /translation="MKYAHKSPAVLLLQDGTVFYGKSAGKIGTATGEICFNTGMTGYQ
                     EIFTDPSYKSQIMVMTSVHIGNYGVCQAESESSSAKISALVCRNFSDHFSRKKADQSL
                     QDYFIENNMVVVSDVDTRAIVQHIRSKGAMNAIVSSEETDLAKLKTLLAKVPSMEGLE
                     LSSTVSTQKAYTYGDAAAALRVAVIDLGVKENTLRCLADRGCYIKVFPMNTSFEEMEK
                     FNPQGYLVSNGPGDPAVMPAMVDTVKKILNSDKALMGICLGHQMLAEACGIKTFKMFN
                     GHRGINHPVKNLVTGRAEVTSQNHGFGVIPEETINKAEIEITHINLNDQSIEGISVKN
                     KKAFSVQYHPEAAPGPNDARYLFDNFVNAVREVKEQAVTV"
     gene            complement(1821..>2367)
                     /locus_tag="PK134_04185"
     CDS             complement(1821..>2367)
                     /locus_tag="PK134_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38077.1"
                     /translation="SLSEQFSPLIGIDITWKNRNDKSSGSKSATERGGRSSGGNFTTR
                     FEYKRDRNISLTLAGIQVTEVKGREFTFGAGYRIPNFKFPFGLFKSVNSRRSNDLNTT
                     LDFSVRKNTTILRKLVEGTNQPTAGLTIISIKLASDYTVSERLNLRLFFEKTINTPVV
                     STSYPNANTAFGITIRFTLSQ"
     assembly_gap    2368..2383
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2384..6764)
                     /gene="sprA"
                     /locus_tag="PK134_04190"
     CDS             complement(<2384..6764)
                     /gene="sprA"
                     /locus_tag="PK134_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRB38078.1"
                     /translation="MINTKEESSVSLNGEFAYLLPGHSKAIGKKGNAYIDAFEGTQST
                     IPLNIAGQWFLASVPRFQPALFPEFDYVSATNDTLGYGYNRAKMAWYNIDPTAFYRSN
                     STVSLSAAERSNHNVRQISEKELFPKRQYSNGIVPYMNTFDISFYPDERGPYNYETSA
                     RAGLSAGIDANGKLVDPTSRWGGFMRKVETSNFEESNIQYIQFWLMDPFNGDAPDSSM
                     SNTGTLYFNLGDISEDVLRDGYESYENGLPTTATPAAYRQNAWGRTPVAQNVLYAFDR
                     DESTRKQQDVGLDGLGNDDEKLHFLTNFLDYIDANVTNQTAKDAIHEDPSSDDFRYFR
                     GDAYTNADGILQRYKNFNGTENNSPVAGSGTFVPSSTQTPDAEDANRINGMEVDENYF
                     QYKVDIDPSKLVVGQNYVTDVLENVAALNLPDASGKTITWYLIKIPVNDPSRERIGQI
                     SDLKSVKFIRVFAKGFNGPKVLRLAKFEFLRGEWRKYDYKLTGILSQPAVFDVGAVSL
                     EENSNREPINYVLPPDFEREIDVTSTNQRQLNEQSLSFKVCELADGDMKVCYKNTTLD
                     MRNYKHVKMVIHAESFLPPANPTNDGELYAVVRLGSDFTDNYYDYRIPLKMTPFGSTD
                     PTVIWPKENDFDIDLDKLTDAKLERNNAGVAFTDEYSKLDGNNVIFIKGNPTLSEVKV
                     MMLGLWNPSGENPAFPPRPPDDGLAKCAEVWFNEFRMSDFNDKGGWATIGRAQVKLAD
                     LGNVSVSGGYSTHGFGSLESKVGERSKEDVTNYDVATSLELGKFFPSSTGLTVPMYFG
                     YGEVFSNPQYDPLDPDIEFSRSISNIDDSKRKQNRKRFSQDYTRRKSLNFTNVKKNKT
                     GKNASKSRIYDIENFNFTYGYTEVYRRNINTEYDIQKDYLGAIGYNFNTNPKPITPFS
                     KMKAKSKYLRPIKDFNFNYMPSTFNFRWDVNRHYGELKVRDVNAVLNQGVSDNSIPVL
                     YDKRFTMNRQYGTSWDITRALKFEFNANNQSKVDEPDGALNSEEKRDSVRKNFFNLGR
                     NTDYMHNASLSYNLPLNKLPITDWISINTRYSTTYHWTASPLIVNTDNGRYYTNPALG
                     NTIQNSNSIQVNNSFNMTSLYNKWKLYKKLTAPPKPKTSAKKEPKVDAKAPDAAKDSL
                     KTKSVKPPEKKKEQEINPAIKALAKVIFSLKNVSFNWNETNGLLLPGFNRQSEWLGQN
                     WDGTSAPGFAFTFGSQNQDIRERAAKNGWLTTDSTFNSQFTSTYTQNITGRASLEPFT
                     GFNIDVNFNRNYSLSTTENYRAYNSPRANGRYYETLARVETGNFSISNLMWGTSFTKD
                     RKDYSSTIFERFAENRRILSERLGNENPNSSGVGADGYRDGYSGTSQEVVILSFLSAY
                     SKKSASRMSLDLFPSVPLPNWTVRYDGLTKIAFVKNLFQSVNLNHGYRSTYNVNSFTT
                     DVSYVPGGNARDINMDFIPKRQIG"
BASE COUNT         1973 a         1386 c         1304 g         2105 t
ORIGIN      
        1 tagctatcct catccgttca ggaacccgtc aggaatctgc tgttgacttg gccaaacgta
       61 ttttaaaaaa tacacataac gagctgtcag aactttctaa gctatccgta aaagacatga
      121 tgaagtataa aggtatgggc aaagtaaagt ctatcactat tgttgctgct ttggaattag
      181 gacgcagacg cgcacaagca caggctttgg aaagacaaaa aataacttca agcaaagatg
      241 cagtagccta ttttaaagcc ttactggccg acctgccgca tgaagaattc agaatacttt
      301 tgttagaccg cgccaatcag attatcaaat ccgaaaacat cagtaaaggt ggtgtttcgg
      361 gaactgtggt tgatgcacga atcattttta aatcggctat agaaaattta gcttcgggca
      421 ttatcctttg tcacaaccac cctagtggca atcctaaacc aagtcaggct gatgttaatc
      481 tgacatcaaa actaagtcag gccggaaaat tactggaaat aaacatctta gatcatgtta
      541 ttattgccgg tgacgactat tacagctttg ccgatgaagg gcagctttga tttttcaaca
      601 gcctgtttac aagtaaaaaa tctgataacc gatttaatgg tacttttgcg aaacttttcg
      661 aaatgaaata cgcacacaaa tcacccgctg tattgttgct tcaggacggt acggtatttt
      721 atggaaagtc ggcaggtaaa attggaactg ccaccggtga aatctgtttt aacaccggca
      781 tgacaggcta tcaggaaata tttaccgacc caagttataa atcgcagatt atggtgatga
      841 cctctgtaca catcggaaat tatggtgtgt gccaggcaga gtcagaatct tcgtcagcaa
      901 aaatatctgc attggtttgt cgcaatttct ctgaccattt ttcacgcaaa aaagccgatc
      961 aaagccttca ggattatttt attgaaaaca atatggttgt tgtatctgat gttgatacac
     1021 gtgccatcgt gcagcacatc cgcagcaaag gtgctatgaa tgccattgtt tcttctgaag
     1081 aaaccgatct tgcaaagctg aaaacccttt tggcaaaagt accttctatg gaaggacttg
     1141 aattatcgag cacagtcagc actcagaaag catataccta tggcgatgca gcagcagcat
     1201 tgcgtgtggc tgtaatagat ttaggagtaa aagaaaacac attgcgctgt cttgctgaca
     1261 gaggttgtta cataaaagtt tttccgatga acacttcttt tgaagaaatg gaaaaattta
     1321 atccgcaagg ttatttggtt tccaatggac cgggcgatcc tgctgtaatg cctgctatgg
     1381 tagataccgt gaaaaaaatt ctgaattccg ataaagcgct gatgggaatc tgtttaggtc
     1441 atcagatgct tgcagaagcc tgtggaataa aaacatttaa aatgtttaac ggacatcgtg
     1501 gcatcaatca cccagtaaaa aatttggtga caggtcgtgc agaagtgaca tcacaaaatc
     1561 atggcttcgg tgttattccg gaagaaacta tcaataaggc agagattgaa attacacata
     1621 taaacctgaa cgatcaaagt atagaaggaa tcagtgtaaa aaataaaaag gctttttctg
     1681 ttcagtatca tcccgaagct gcacccggcc ctaatgatgc gcgttatctt ttcgacaact
     1741 tcgtaaatgc cgttagggaa gtaaaagagc aggctgttac ggtgtagcct gccctcttca
     1801 attattttat tttaggactc ctactgcgac agtgtaaatc ttattgtaat accaaatgca
     1861 gtattcgcat tggggtaaga tgttgacacc acaggtgtat tgatagtctt ctcgaagaac
     1921 aatcttaaat taagtcgttc acttaccgta taatcagaag caagcttaat agagatgatg
     1981 gttaaacctg ctgttggctg attggtacct tcaaccaatt ttctgagaat ggtagtgttc
     2041 ttacgaactg agaaatctaa tgttgtattc agatcgtttg atctgcgtga gttaacactc
     2101 ttaaacagtc cgaaaggaaa cttaaagttt ggaattcgat agcctgctcc aaatgtaaac
     2161 tcacgtcctt taacttctgt tacctgaata cctgcaagtg ttaatgatat atttctatca
     2221 cgcttgtact cgaaacgtgt ggtgaaattt ccaccggaag aacgtccgcc acgctctgtt
     2281 gccgacttac ttcctgatga tttatcattt ctgtttttcc aggtgatgtc tattccgatt
     2341 aaaggactaa actgttcact caaactcnnn nnnnnnnnnn nnngccctat ctgtctctta
     2401 ggaataaaat ccatgttgat gtcgcgcgca ttaccaccgg gaacataaga aacatcagta
     2461 gtaaagctgt ttacattgta agtagaacga taaccatggt taaggtttac actctggaat
     2521 agatttttta caaatgcaat tttggtaagt ccgtcatagc gcactgtcca gttaggtagt
     2581 ggtaccgatg gaaacaaatc gagcgacatg cgtgaggcac tcttcttaga ataagctgaa
     2641 aggaatgaaa gtatcactac ctcttgtgat gttccactgt agccatctct gtagccatca
     2701 gcgccaacac ctgatgagtt tggattttca ttgcccaatc tttcagaaag tattcttctg
     2761 ttttctgcaa accgctcaaa gatggttgat gaataatctt ttctgtcttt agtgaatgat
     2821 gttccccaca tcagattgct gatactgaaa ttaccggttt ctactcttgc caatgtttca
     2881 taataacgac cgttagcacg cggactgttg taggctctgt aattttctgt cgtacttaaa
     2941 gagtaattgc ggttaaaatt tacatcaata ttaaatcctg taaaaggctc aagagaagct
     3001 cttcctgtga tgttttgtgt gtaggtagaa gtaaactgac tgttgaaagt tgagtctgtt
     3061 gtaagccatc cattcttggc cgcacgttct ctgatgtcct gattctgact accaaaagta
     3121 aatgcaaatc ccggtgcact ggtgccatcc caattctgac ctaaccattc actctgtctg
     3181 ttaaagccgg gcaacaacaa tccattggtt tcattccagt taaacgaaac attctttaaa
     3241 ctgaaaataa cttttgccaa tgcttttata gcaggattta tttcctgttc ttttttcttc
     3301 tccggtggtt taacactctt tgtctttaaa ctgtctttcg cagcatccgg tgctttagca
     3361 tcaacctttg gctctttctt agcagatgtt tttggctttg gtggtgctgt caacttttta
     3421 tacagtttcc acttattgta taaagaagtc atgttaaagc tgttgttaac ctgaatagaa
     3481 tttgagtttt gaatagtgtt gcctaaggca gggtttgtat agtaccttcc attgtctgtg
     3541 tttactatta aaggtgatgc tgtccagtga taagtagtgc tgtagcgcgt attaatgctt
     3601 atccaatctg ttatcggcaa cttatttaat ggaagattat acgataaaga tgcattgtgc
     3661 atgtaatctg tattacgtcc taagttgaaa aagttttttc ttacagaatc gcgtttttct
     3721 tctgagttta aagcaccatc gggctcgtca accttgcttt gattgttggc attaaactca
     3781 aacttcaatg cgcgggtgat gtcccaactt gttccatact gacggttcat tgtaaaacgc
     3841 ttatcataca acacaggaat agaattatca gaaacaccct gattcaatac agcatttaca
     3901 tcacgcactt taagttcgcc atagtgtctg tttacatccc atcggaaatt aaaggttgat
     3961 ggcatgtagt tgaaattaaa atctttaata ggacgtaggt acttcgactt agccttcatt
     4021 ttactgaaag gcgtaatcgg ttttgggttt gtattaaaat tataacctat tgcaccaagg
     4081 tagtctttct gaatatcata ttccgtattg atatttctcc gataaacttc agtatatcca
     4141 taagtaaaat taaagttctc tatgtcataa attcttgact tagatgcatt cttccctgtc
     4201 ttatttttct tgacattggt aaagttcaaa ctctttcttc gagtataatc ctgcgaaaaa
     4261 cgttttctgt tttgtttgcg ttttgaatcg tcaatgttgc tgattgaacg gctgaattca
     4321 atatcaggat caagaggatc atattgcgga ttgctgaata cttcaccata accaaaatac
     4381 attggcactg taagacctgt acttgatgga aaaaacttac ccaactcaag tgatgtggct
     4441 acatcatagt ttgtcacatc ttctttgcta cgctcaccaa ctttactttc taaacttccg
     4501 aatccatgag ttgaatagcc accggaaaca gaaacattac ccaaatcggc aagcttcacc
     4561 tgcgcacgac ctatagttgc ccatccgcct ttatcattaa agtcggacat gcggaattcg
     4621 ttgaaccata cttctgcaca cttcgccaat ccgtcatccg gtggtcgtgg cgggaatgca
     4681 ggattctcac cacttgggtt ccacaatcca agcatcataa ctttcacttc gctcaatgta
     4741 ggattacctt taatgaaaat tacattgttg ccatcaagct tagaatattc atctgtaaat
     4801 gcaacacctg cattattacg ctccagctta gcatcggtca gtttatccaa atcaatatca
     4861 aaatcattct ctttaggcca aattactgta ggatctgtac ttccaaaagg agtcattttt
     4921 aatggaatgc ggtagtcgta gtagttatct gtaaagtcag agcctaaacg aacaactgcg
     4981 taaagctcac catcgttggt tggattggca ggtggtagaa acgattcggc atggataacc
     5041 atttttacat gtttgtaatt acgcatgtcg agtgttgtgt ttttataaca caccttcata
     5101 tcaccatcag caagttcgca cactttaaac gaaagcgact gctcgttaag ttggcgttga
     5161 ttggttgagg taacatcaat ttcacgctca aagtccggag gcaaaacata attaatcggc
     5221 tcgcggttgc tgttctcttc gagtgaaaca gcacctacat caaaaacagc aggctgacta
     5281 agaatacctg taagcttata atcatacttc ctccattcac ctctcaggaa ttcaaatttt
     5341 gcaaggcgca gcaccttggg tccgttaaat ccttttgcaa aaacacggat aaattttacg
     5401 gacttcagat ctgagatttg accaatgcgc tcacgacttg gatcgttcac cggaattttt
     5461 atcagatacc atgtgatggt cttacctgat gcatcaggaa gatttaatgc agctacattc
     5521 tccagcacat ctgttacata gttttgtcct actaccaatt ttgaaggatc tatatcaact
     5581 ttatattgaa aataattttc gtcaacttcc ataccgttaa tacggtttgc atcttctgca
     5641 tctggagttt gtgtacttga tggcacaaat gttccactgc ctgcaaccgg tgaattgttt
     5701 tctgtcccgt tgaagttttt ataacgctgc aatattccat cggcattggt ataagcatca
     5761 cctctgaaat atctgaaatc atctgacgaa gggtcttcat gaatggcatc ctttgcagtt
     5821 tgattcgtta cgttggcgtc aatgtaatcg aggaagtttg tgaggaaatg tagtttctca
     5881 tcatcattac ccaaaccatc taaaccaaca tcctgttgtt tacgtgtact ttcatctctg
     5941 tcaaaagcgt aaagtacatt ctgtgcaaca ggtgtgcggc cccatgcatt ctgacgatat
     6001 gctgccggtg ttgctgttgt tggaagacca ttctcataac tttcatatcc atcacgcaac
     6061 acatcttcag agatatcacc caagttgaaa taaagcgttc ctgtatttga catactggaa
     6121 tcaggtgcat cgccattaaa aggatccatc aaccaaaact gtatgtactg aatgtttgac
     6181 tcttcaaagt tgctcgtttc tacttttcgc ataaaaccac cccaacgtga tgttgggtca
     6241 accaactttc cgttagcatc aatacctgca gataaacctg cacgtgcaga tgtttcataa
     6301 ttgtaaggtc cacgctcatc aggataaaaa gaaatatcga atgtattcat gtaaggaaca
     6361 ataccattgc tgtattgtcg cttggggaat aattcctttt cactaatttg tcttacatta
     6421 tgattacttc tttctgcagc agataatgac accgtactgt tgcttctgta aaaagcagtc
     6481 gggtcaatat tgtaccaagc cattttagca cgattgtagc cataacctaa tgtgtcgttt
     6541 gtggcactca catagtcaaa ttcaggaaac agtgcaggct gaaaacgtgg cacagaagct
     6601 aagaaccact gacctgctat attcaacgga atggtactct gtgttccttc aaaggcatca
     6661 atataagcat tacctttttt accaattgct ttactatggc cgggaagtaa ataagcaaat
     6721 tctccgttca atgaaactga agactcttcc ttagtattaa tcaatggaat tttatcaacc
     6781 aact
//
LOCUS       DAORQT010000079         6758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1762_length_6758_cov_7.399373, whole genome shotgun sequence.
ACCESSION   DAORQT010000079 DAORQT010000000
VERSION     DAORQT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1762_length_6758_cov_7.399373"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>553)
                     /locus_tag="PK134_04195"
     CDS             complement(<1..>553)
                     /locus_tag="PK134_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HRB38079.1"
                     /translation="YVAPGKYDEFYNIVSGGFSGQMSIYGLPSGRLFRVIPVFSVDPE
                     KGYGYSEESKAMLNTSHGFVPWDDLHHISLSETDGIHDGRWAFGNANNTPRIARIDLK
                     TFRTAEIIEIPNSAGNHSSPFITENSEYVVAGTRFSVPMTNGTTDVPINTYKENFKGT
                     VSFINVDKESGKMGIAFQILLPGV"
     assembly_gap    554..584
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(723..1235)
                     /locus_tag="PK134_04200"
     CDS             complement(723..1235)
                     /locus_tag="PK134_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB38080.1"
                     /translation="MKSIQKHLINLMIAGMGITLFAACGGSAPKEEKAAESKEMNQYY
                     EEEKQKEDTKVADDGKGLGKFTDVKVDPKLDKQMADAGKSVYDVKCSACHKLTDEKLV
                     GPGWKGVTGKRSVEWIMNFSTNPDEMLDKDAIAKGLLEECLVRMPNQGLSDDDARHVY
                     EYMRENDGVK"
     gene            complement(1455..1622)
                     /locus_tag="PK134_04205"
     CDS             complement(1455..1622)
                     /locus_tag="PK134_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38081.1"
                     /translation="MLFAIFSLHNFREPGNLDAIDDRHCKKKDRLRALYIITGAIVVA
                     LLYTWLISVFC"
     gene            1955..>2224
                     /locus_tag="PK134_04210"
     CDS             1955..>2224
                     /locus_tag="PK134_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HRB38082.1"
                     /translation="MKKTAVIFFILFSQLITVFGQSQDSLMLRKIYNESLEHGQAYNY
                     LKVLCKQIGHRLSGSPEAEKAVKWAEKSMKDFGADTVFLQNIMVPH"
     assembly_gap    2225..2276
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2277..3326
                     /locus_tag="PK134_04215"
     CDS             <2277..3326
                     /locus_tag="PK134_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRB38083.1"
                     /translation="PLNVLALGGSVGTNGKTIAANVVEVTSLDELDKLGTSKCAGKIV
                     FINQHMDATKIMTFDAYGPAVTIRWAGAKRAAPLGAVAVIIRSVNPSLDDFPHTGTLH
                     YHDTIKKIPACAVSTNDAEKLSAMLKSDPQLKIKLKMNCMQLPDAPSHNVIGEIKGAD
                     ANAGYLIVGGHLDSWDTGEGAHDDGAGVVQAMEVLNLLKKAGIKPRHTIRAVAFMNEE
                     NGGKGGEYYAKNALEKNEKHIAAIESDCGGFTPRGFFLQGDSLKKEKIRSWKNLFESY
                     QIHSFNFDGGGADIGHLKNQNTFLLGLLPDTQRYFDIHHSPNDVFEHVNRRELQLGAA
                     TMAAMIYLIDKYGLE"
     gene            3326..4465
                     /locus_tag="PK134_04220"
     CDS             3326..4465
                     /locus_tag="PK134_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRB38084.1"
                     /translation="MLLKETELKQNAAAYAELKKNISSETTPIFLDRKKEIFSDYDLA
                     TRRAAYYRWKSTESLDKLLIAFEANAIKAGIKVLWAVDTAQAHTELNAVIDKFQQHKI
                     HIHTCYNELETEKLNQPSPNSIKNKISICDAIFVSAEPAMIACLSDKNFYESDVKIIL
                     ASVDRLISSVNELELLMSLQSLQRNNQRESFSLISNAGNNSHKQEVYVLIFDNGRSKL
                     LESQTERQMLHCIDCGICQTVCPVTESIGTTGGNENNYGPPSYIRAVFDGGQREYGHY
                     AGLCNMCGKCNVHCPVHIDFKNSFLHLRHENVQQKHRSQKERLFYMVWKKTMLKREFM
                     NWKTVNPLRYAVETLFLKSKTGVRKLPQPAAKSFNQMWREKMGMK"
     gene            complement(4468..5505)
                     /locus_tag="PK134_04225"
     CDS             complement(4468..5505)
                     /locus_tag="PK134_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRB38085.1"
                     /translation="MQLRNLVSKQLLKKRLTEETFNRKTVSFYRYLKIEDVENFRNRL
                     YIDFEALNIFGRVYVAAEGINAQISVPEQNWELFKNYLEAQEQLKNMPLKIAVEDNGK
                     SFYKLIVRIKSKIVADGLNDNTFDVTNVGNHLSAKEFNEAMQQSGTIVIDMRNHYESE
                     VGFFKDALKPDADTFRDALPQAIEMTKDNKDNKILLYCTGGIRCEKASAYFKHHGYKD
                     VNQLYGGIIQYAREVNAEHLENKFLGKNFVFDERMGERISDEIVSTCHQCGGKCDTHV
                     NCANDDCHLLFIQCESCKATYEGCCTPECKAIINLPEEEQKRLRKGRVKKQAHAVYKS
                     RLRPDLKKILK"
     gene            complement(5541..5780)
                     /locus_tag="PK134_04230"
     CDS             complement(5541..5780)
                     /locus_tag="PK134_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38086.1"
                     /translation="MKRLLILLIIFLSALTFQNVNAQCSVCRAGAESSVEQGYKAGKG
                     LNKGILYLMAVPYVMVGVGFYFFYNKIKKKETKEG"
     gene            complement(5782..>6758)
                     /gene="infB"
                     /locus_tag="PK134_04235"
     CDS             complement(5782..>6758)
                     /gene="infB"
                     /locus_tag="PK134_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRB38087.1"
                     /translation="TVVESSLDKGRGYIATVLVQSGSLKIGDVVLAGCYSGRVKAMFN
                     ERGNKRNNAGPATPVLILGLNGAPQAGDQFHVMDDEREAREIANKRLQLQREQGIRTH
                     KHITLDEIGRRLAIGNFKELNVIVKGDVDGSIEALADSLLKLSTAEVQVNVIHKSVGA
                     ITESDVLLASASNAIIVGFQVRPSVNARRLAEKEEIEIRLYSIIYDAINEIKAAMEGM
                     LAPEFEEKIVCNIEVRETFKIPKVGTIAGCMVLDGKMTRNTAIRVIRDGIVAYTGKLA
                     SLKRFKDDVKEVSAGFECGLNIEGYNDIKVGDIIEGYEQVAVKRTLTV"
BASE COUNT         2102 a         1263 c         1168 g         2142 t
ORIGIN      
        1 acaccgggta gtaatatctg gaatgcaatt cccatttttc ctgattcttt atctacattg
       61 ataaagctta cagtaccttt aaaattctct ttataagtat taatcggaac gtctgttgtt
      121 ccattagtca tcggtacact aaaacgggta ccggcaacta catactcaga attttcagta
      181 ataaatggtg aggagtgatt tcctgcactg ttaggaattt caatgatttc tgctgttctg
      241 aaagttttca aatcaatacg tgcaatacgc ggagtgttgt ttgcattgcc aaaagcccag
      301 cgaccatcat gaataccatc agtttctgat aaagaaatgt ggtgcaaatc atcccaggga
      361 acaaatccat gtgaagtgtt tagcatagcc ttagattctt cgctgtagcc atatcctttt
      421 tcaggatcta cagaaaaaac aggaattacc ctgaacagtc ttccagaagg caatccataa
      481 atactcattt gcccactaaa acctcctgat acgatgttgt aaaactcatc atatttaccg
      541 ggagctacat aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcatca cccgatgtgg
      601 ctgtactcac atttttaggc ttgcacgatg gcattacagc aattgtaatt acagctgctg
      661 atgccaataa tacattttta atttccgatt gtttcataat atttaattta ttttatagtt
      721 ccttacttta caccatcatt ctcacgcatg tactcataga catgacgtgc atcatcatca
      781 ctcaaccctt gattaggcat acgcaccaaa cattcttcca atagtccctt tgcaatagca
      841 tctttatcta acatttcatc agggtttgta gaaaagttca taatccactc tacagatctt
      901 tttccggtaa cacctttcca gcccggtccc acaagtttct catcggttaa cttatgacag
      961 gctgaacatt ttacatcata aacggattta cctgcatctg ccatttgttt atcaagtttt
     1021 ggatctactt ttacatcagt aaactttcca agaccctttc catcatctgc taccttagtg
     1081 tcttcttttt gtttttcttc ttcgtaatac tgattcattt cttttgactc agcagctttt
     1141 tcttcttttg gcgcagaacc gccacaagca gcaaaaaggg ttatgcccat tccggcaatc
     1201 attaaattga ttaagtgttt ttgaattgat ttcatattga tgatttgaat taaaatttta
     1261 cgtgacaaaa gtataaataa ttaacagggt gttcaaaata agttaatatg attgatgtca
     1321 cataaactat tgataattac atgtagcgta aactgctttt cagcatgttt ccttaatgaa
     1381 tctaaacccc ctaaaaaagt atcaggataa aataaatata ctgtgtgttt tgctgtccgt
     1441 tttagcaata cattttagca gaaaacagaa atcaaccagg tatagagtaa tgcaacaact
     1501 atagctccag taataatata taaagctctc aaacggtctt tctttttaca atgtctgtca
     1561 tcaatagcat caagatttcc aggttcgcga aaattatgca gactgaaaat cgcaaacagc
     1621 acgcaaaatc tgctaacaac ttcaacgaac agaattaatg ttgctaatgt tgtcaggtaa
     1681 aggagataaa aaatccaaat gctatgaatg aaatgaacag ttattgttaa taatatactg
     1741 atcagtagaa acgaaatcag aaatttttgc cttattctga tctcagcttc acctatgtta
     1801 caacacccgg ctttatactg atttgtgtac attgtatcta ttattaaacc ttaacatcaa
     1861 agttccatta ttgttctgta aaaatattga atgaagtgaa aatattatag actctcttta
     1921 tttactttca cagccaaaat agggaaagag aatgatgaaa aaaacggcag ttattttttt
     1981 tattttattc agtcagttga taactgtttt tggtcagtcg caggattctt tgatgcttcg
     2041 aaaaatttac aatgaatcgt tagagcacgg acaggcatat aattacctta aagtgttgtg
     2101 taaacaaatt ggacataggc ttagcggttc accagaggct gaaaaggcag taaagtgggc
     2161 cgaaaaatca atgaaagatt ttggtgcaga cacggttttt cttcagaaca tcatggtacc
     2221 gcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccat
     2281 taaatgtgct tgctttaggc ggaagtgttg gaacaaatgg aaaaacgatt gcagccaacg
     2341 ttgtggaggt aacttcatta gatgagctag ataaattagg tacttcaaaa tgtgcaggta
     2401 aaatagtttt tatcaatcag cacatggatg caactaaaat tatgacattt gatgcctacg
     2461 gacctgctgt tactattcgc tgggcaggag ctaaaagggc tgcaccatta ggtgctgtgg
     2521 ctgtcattat tcgatcagta aatccttcat tagacgattt tccacataca ggcacattac
     2581 attatcatga tacaattaaa aaaatccctg cttgtgcagt aagtacaaat gatgctgaaa
     2641 aattatcagc aatgcttaaa agcgatccgc aacttaaaat aaagctgaag atgaactgta
     2701 tgcaacttcc tgatgcacca tcgcataatg ttattggtga gataaaaggc gcagatgcaa
     2761 atgcaggata tctaattgtt ggtggacatc ttgactcatg ggatacaggc gaaggtgcac
     2821 atgatgatgg tgcaggagtg gtacaagcca tggaagtatt aaacctgctt aaaaaagcag
     2881 gtattaaacc acgtcatact attagggctg ttgcttttat gaatgaagaa aacggaggaa
     2941 aaggcggaga gtactatgca aaaaatgctc tggaaaaaaa cgaaaaacat attgcagcta
     3001 ttgaatctga ttgcggaggt tttactccaa gaggattctt tttacaaggt gattccttga
     3061 aaaaagaaaa aataaggtca tggaaaaatc tttttgaatc ttatcagatt cacagtttta
     3121 attttgatgg cggaggtgcg gacatcggtc atttaaaaaa tcaaaatact tttttgttag
     3181 gcttgttgcc tgacacacaa cgttattttg atattcatca ttcgccaaat gatgtgtttg
     3241 aacatgttaa taggcgcgaa ttgcagttag gtgcggcaac tatggctgcg atgatttatt
     3301 taatagataa atacggattg gagtaatgct gttgaaagaa actgagctga aacaaaatgc
     3361 agcagcttat gctgaactga aaaagaatat ttcttctgaa accacaccaa tatttttaga
     3421 cagaaaaaaa gagatttttt ctgattatga tctggcgaca cgcagggctg catattatcg
     3481 ctggaaaagc acagaaagcc tcgacaaact cctcatcgcc tttgaggcca atgcaattaa
     3541 agcaggaata aaagttttat gggcagttga tactgctcag gcacatacgg aactgaacgc
     3601 tgttattgat aaatttcaac agcataaaat tcatatccac acatgctaca acgagcttga
     3661 aacggaaaaa ttaaatcagc cttcacctaa cagtattaag aacaaaataa gtatttgtga
     3721 tgcaattttt gtttcagccg aaccggcaat gattgcctgt ttatctgata aaaattttta
     3781 tgaaagtgat gtaaaaataa tattagcatc ggttgacaga ttgatttctt ctgtcaatga
     3841 acttgagttg cttatgtcgc tacaatcgct tcaaagaaac aatcaaagag aatctttttc
     3901 tttaatttct aatgcaggaa ataacagcca taagcaggaa gtttatgttt tgatttttga
     3961 taatggaaga agcaaattat tagagagtca gacagaaagg caaatgctac attgcattga
     4021 ttgtggaata tgtcagacag tatgtcctgt aacagaaagt attggcacaa caggaggcaa
     4081 tgaaaataat tacggtccgc catcatatat cagagcggtt tttgatggtg gacaacgtga
     4141 atatggacac tatgcaggat tatgcaatat gtgtggtaaa tgcaatgtac actgccctgt
     4201 acatattgac tttaagaact cttttctaca tctgcgtcat gagaatgtgc agcagaaaca
     4261 ccgttcgcag aaggagcgac ttttttatat ggtttggaaa aaaacgatgc ttaaacgaga
     4321 gtttatgaat tggaaaacgg ttaatccgtt acgttatgct gtagaaacac tttttctgaa
     4381 atcaaaaaca ggagtacgaa aacttccaca gcctgctgcc aaatcattta atcagatgtg
     4441 gcgcgaaaaa atgggaatga aataatttta tttcagaatt tttttcagat caggccttag
     4501 cctgcttttg taaacagcat gtgcttgttt tttaacacgc cctttgcgaa gtcgtttttg
     4561 ttcttcttca ggaaggttaa taatggcttt acattctgga gtacagcaac cttcataagt
     4621 tgctttacac gattcgcatt gaataaacaa taagtggcaa tcatcatttg cacagtttac
     4681 atgtgtatcg catttgcctc cgcattgatg acacgtactg acaatttcat cacttattct
     4741 ctcacccatt ctttcatcaa acacaaagtt tttacccaga aatttatttt ctaaatgttc
     4801 agcatttact tctcttgcat attgaatgat gccaccataa agttgattta catccttata
     4861 accatgatgc ttaaaataag cacttgcttt ttcgcaacga ataccaccgg tgcagtacaa
     4921 taaaatttta ttatccttat tatcctttgt catttcaatt gcttgtggta aagcatcacg
     4981 aaatgtatca gcatcgggtt ttaaagcatc tttaaaaaat cctacttcac tttcgtaatg
     5041 atttcgcata tcaataacaa tcgttcctga ctgttgcata gcctcgttaa attcttttgc
     5101 agaaagatga ttacccacat tagtaacatc aaatgtattg tcgtttaaac catcggcaac
     5161 tatttttgat tttatgcgaa cgataagttt gtagaacgac ttgccattat cttcaacagc
     5221 aatttttaat ggcatattct taagctgctc ctgcgcttca agataatttt taaacaattc
     5281 ccagttttgt tcaggaacgc ttatttgtgc attaatgcct tcggctgcta catacactct
     5341 tccgaatatg tttaaagctt cgaagtcaat ataaagacga tttcggaaat tttcaacatc
     5401 ctcaattttt aaatacctat agaaagaaac agttttcctg ttaaatgttt cttcggtaag
     5461 acgttttttg aggagttgct tacttacaag gtttcgtaat tgcatgttac gtgttgtaat
     5521 taaacagtta gatataatga ttaaccttct ttggtttctt ttttcttgat tttattgtaa
     5581 aaaaaataaa agccgacacc aaccatgacg tatggaacag ccattaaata aagtattccc
     5641 ttattgagac ctttaccggc tttgtatcct tgttcaacgc tgctttctgc acctgccctg
     5701 caaacgctgc attgtgcatt tacattttga aaggtaagtg ccgacaagaa tataattaat
     5761 aaaattaaaa gccgtttcat attatacagt taatgttcgt ttaacggcaa cctgctcata
     5821 gccttcaata atgtcgccta ctttaatgtc gttataacct tctatattta atccacactc
     5881 aaatccggca gaaacttctt ttacgtcatc tttaaaacgt ttgagtgatg caagcttgcc
     5941 tgtataagct actataccat cacggataac tctgatggca gtatttcttg tcatttttcc
     6001 atcaagcacc atacaacctg caatagttcc aacttttggt atcttaaatg tttcacgcac
     6061 ttcaatattg cagacaatct tttcttcaaa ctcaggtgca agcattcctt ccatagccgc
     6121 tttaatttca ttaatagcgt cataaataat gctgtaaagc ctgatttcaa tctcttcttt
     6181 ttctgcaagt ctgcgtgcat ttactgaagg tcgcacctgg aaaccaacaa tgatggcgtt
     6241 agatgctgat gctaagagaa catcagattc tgtaatggca ccaactgatt tgtgaatgac
     6301 attaacctgc acttctgctg ttgaaagttt aagcagcgaa tcggcaagag cttcaataga
     6361 accatctacg tcacctttta cgattacatt aagttcttta aagtttccga ttgccaaacg
     6421 tctgccaatt tcatcaagtg taatatgttt gtgtgtacga ataccttgtt cgcgctgtag
     6481 ctgcaatctt ttgttggcaa tttctcttgc ttctctttca tcatccatca catgaaactg
     6541 atcaccggcc tgtggcgcac cattaagtcc aagaatcaaa accggggttg ctggtcctgc
     6601 attatttctt ttatttcccc gttcgttgaa catggctttc acacgaccac tataacaacc
     6661 ggcaagtact acatctccaa tcttcaatga accggattga actaatactg ttgcaatgta
     6721 gcctcttcct ttatctaatg atgattctac tacagtac
//
LOCUS       DAORQT010000080         6724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1780_length_6724_cov_7.297946, whole genome shotgun sequence.
ACCESSION   DAORQT010000080 DAORQT010000000
VERSION     DAORQT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1780_length_6724_cov_7.297946"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="PK134_04240"
     CDS             complement(<1..1948)
                     /locus_tag="PK134_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38088.1"
                     /translation="MFLFADSAKATHIVGGEIFYSYLGNGNYQVTMYIYRDCANGVPP
                     FDDPAAIGIYDQSFNLEQALFVDVRPDSLLIPSTINNPCFIPPVNICYRRATYIFNVN
                     LPPSSGVYYIAYQRCCRNNTINNLIGPGVTGATYVGEIRASSFFNNSSPRFKFLPPPF
                     VCLNFPFVFDHSATDPDNDTIRYSLCTPLVGGDTIVPAPLPPVSAPPYNNVIFAPPYS
                     VNNMLNGTPGIQPLSIDSITGVLTATPNTIGQFVIGVCAKEYRNNVYLGQTRRDYQLN
                     VVPCPSLVVAAFLNPIVSCGSNTVSFQNGSIGAISYHWDFGVPNLTNDTSNAFNPVYT
                     YPDTGTYNVTLIAYSLFNPGCADTIHGVVTVTKELITDFTSTVQACTTVALFNDTILS
                     SAPASFSYNWNFGNGTTSNVSNPQVTYGSPGNYPVTLVVTSSLGCKDTVTKTINIQQF
                     MSATATTIQNVRCKGECNGSGLVAVTNNNGPYTILWSNPPGQSNDTIVNLCQGNYSGT
                     VTDSAGCTSNFSLNISEPPQLTLSATATPDYCSGQCIGTATALAGGGTPPYNFVWSNG
                     SNSGSLNQLCAGNYTVSVTDSKGCTTSPVTVSVLFSDSLAALDVVPSIDTIYRGQSVN
                     LSATQNTGYIYNWQPPTYLNSNGIA"
     gene            complement(2133..3950)
                     /locus_tag="PK134_04245"
     CDS             complement(2133..3950)
                     /locus_tag="PK134_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38089.1"
                     /translation="MKIMNRSIVSFLILFWFCGSAIAQTDTTKNKSVDFYQGVEQVSK
                     SSKLLRSIYKMIFRPVTAKAVKVKKKPVVQEINFSEFQCLPVGNISITVFDPFGYSLA
                     DSSIKKQPSVINAGNYLHNKTSNRQIRKLMIIETGDEFDSLLVSESERLIRAQPYIRD
                     AIIIPHKNELNQTIDLEVRVLDNWSILIGGGMSETNTKISVTERNFAGLGNTFENSYR
                     WYYNKNADIIESSYEVPNLLGTHADAKILYHLDEREGDVKSIGVERPFYSPLTKYAGG
                     ILLEQHFVKAVYGEPSENYSERYKYNFIDAWSGYSMRLLSGNKVYQRNTRLIAALRGS
                     SVQYLSFPSAGFDSLGLLKNQNTYLAGIGFSSREYITERYVFKYGQKEDIPVGRTYGL
                     TVGYRERASSGNWYAGAMAAFGDYFSFGYLSSLVRYGTFFYNGNARNSAFEIQTVYFT
                     RQMAIGKWRLRQFLRANATLGIDRITNEFVSLEDQMRGFDSRTSGTKRIFISLQTQTY
                     APYDFLGFKFGPYLIASAGFIGNQLHGFNRSRMYSVFGVGMIIKNEYLALSSFQISLA
                     YYPTIPYSGQDVFRVNPLRSTDFQLVDFDLKKPDIIEFK"
     gene            complement(4027..>5091)
                     /locus_tag="PK134_04250"
     CDS             complement(4027..>5091)
                     /locus_tag="PK134_04250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRB38090.1"
                     /translation="DTQKYIGDAAKKAIDDGFTKYPPISGYADVREAISQKLKRENNL
                     DYKASQIVVSTGAKQCIANAVLCCVNPGDEVLVPTPYWVSYSQIIQLAGGVPIYLNTT
                     IDSDFKVTPQQVEKAITSKTRMFIFLSPCNPTGTVYSHDELKALAAVFAKHENIFVLS
                     DEIYEYINFVGKHHSIASFEELKNQVIIINGVSKGYAMTGWRIGYMAAPQDVATACDK
                     MQGQFTSAASSIAQKAAKAAISISTPDRDMMVETFRKRRDLVLNLMKDIPGWKTNTPE
                     GAFYVFPDISYYFGKTDGSTVINNPEDMCMYLLKDAKVALVTGEAFGDNQCIRFSYAT
                     SDKLLTEAIARIKVSLAKLK"
     assembly_gap    5092..5101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5321..6508)
                     /gene="kbl"
                     /locus_tag="PK134_04255"
     CDS             complement(5321..6508)
                     /gene="kbl"
                     /locus_tag="PK134_04255"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRB38091.1"
                     /translation="MYNTLKPVLEKELEEIKSAGLFKQERIISTPQAADITANGKEVI
                     NFCANNYLGLSSHPKVVEAAHAAINSHGYGMSSVRFICGTQDIHKTLESKIASFLETE
                     DTILYAAAFDANGGVFEPLLNEQDAIISDELNHASIIDGVRLCKADRHRYKHNDMADL
                     EEKLKATMNKRHRMIVTDGVFSMDGTIAQLDKICDLADRYQALIMIDECHASGFMGKT
                     GRGTHEHHGVMGRIDIITGTLGKALGGAMGGFTSGRKEIIEMLRQRSRPYLFSNSLAP
                     SIVGASIAVLDMLSQTTTLRDKLWDNTQYFRKQISEAGFDIKPGQHPIVPIMLYEAQL
                     AQQFAAMLLDEGIYVTGFFYPVVAKGNARIRVQLSAGHERHHIDKALNAFIKVGKKLG
                     VIK"
BASE COUNT         2080 a         1331 c         1237 g         2066 t
ORIGIN      
        1 ttgcaatacc gttgctgttt aaataagttg gtggctgcca gttgtaaatg taacctgtat
       61 tttgagttgc tgataggtta acactttgac cacgataaat ggtatcaata gaaggcacaa
      121 catctaatgc tgctaacgaa tcagaaaaca aaacacttac ggttacagga gaagtagtac
      181 aaccttttga gtcagttaca gaaacagtat agttgccggc acaaagctga ttgagagaac
      241 ctgaatttga accattactc cagacaaagt tgtatggtgg tgtgcctcca cctgccaatg
      301 ctgtggctgt gccaatacat tgaccgctgc aataatcagg agttgcagtt gcagataagg
      361 taagttgtgg tggttcagaa atatttaaag aaaaatttga agtacatcct gcagaatctg
      421 ttacagtacc tgaatagttg ccttgacata gattaacaat agtatcattg gattggcccg
      481 gaggattact ccacaatata gtgtatggcc cattattgtt tgttactgcg acaaggccgg
      541 aaccgttaca ttcgcctttg caacgtacat tctgaatggt agttgcagtt gcactcatga
      601 attgttgtat gttaatagtt ttggtaacgg tatccttaca accgagcgaa cttgtaacaa
      661 ctaaggtaac cggataatta cccggtgagc cataggtaac ttgcggatta cttacatttg
      721 aagttgttcc attaccaaaa ttccaattgt aagaaaatga tgcaggtgca gatgataaaa
      781 tagtgtcgtt aaataatgct actgttgtac atgcctgaac tgtagatgta aaatctgtta
      841 tcagttcttt agttaccgtt actacaccat gaatggtgtc ggcacagcca ggattaaata
      901 aagaataggc aattaatgta acgttatacg tccctgtatc gggataagta taaaccggat
      961 taaaagcatt agaagtatcg ttggtgagat tgggtacacc aaaatcccaa tgatatgaaa
     1021 tggcaccaat gcttccattt tgaaatgaaa ctgtattact gccacaagaa actattggat
     1081 ttaaaaaagc ggcaactacc aatgacggac aaggcactac gtttaactga taatctcttc
     1141 ttgtctgacc taagtaaaca ttgttgcgat attcttttgc acagacacca atcacaaact
     1201 gacctattgt gtttggagtt gcagttagca cacctgttat tgaatcaata ctcagtggtt
     1261 gaattcccgg tgtaccattg agcatattgt tcacagaata aggtggtgca aatattacat
     1321 tattgtatgg tggtgcagaa accggtggaa gtggtgccgg aactatggtg tcgccaccaa
     1381 ctaagggtgt gcatagtgaa taacgaatgg tgtcattatc aggatctgtt gcagaatggt
     1441 cgaaaacaaa agggaaattt agacaaacaa acggtggtgg caaaaattta aaacgagggc
     1501 tactgttgtt aaaaaaactg cttgctctta tttcgccaac ataggtagca ccagtcacac
     1561 caggtccaat taaattattg atggtattat tccggcagca acgctgatag gcaatatagt
     1621 aaacacctga agaaggtggt agattaacat taaaaatata ggtagctcgc ctgtaacaaa
     1681 tattcacagg tgggataaaa catggattgt tgatagttga aggaataaga agagaatcag
     1741 gtctaacatc aacaaataat gcttgctcta agttaaaact ctgatcgtag ataccaattg
     1801 cagcaggatc atcaaaaggt ggtacaccat tggcacagtc acgatagata tacattgtga
     1861 cctgatagtt tccattacca aggtagctgt aaaaaatctc tcctccaaca atatgcgttg
     1921 cctttgcact gtctgcaaac agaaacaaaa aatttattaa taaacagaac ttccaaaagt
     1981 ggcttttcat ataagatgga aaggtaacaa agatactaaa attgctttct atataatgac
     2041 tacaaaaatt aaaatatcat tgtctttaat cggtaatttt tctgttttaa aaaatagaaa
     2101 cttcaaaaca taaaacttgc taaagtggaa tattatttga attcaataat atcaggtttt
     2161 tttaaatcga aatcaaccaa ctggaaatct gtgcttctaa gcggattgac tctgaacaca
     2221 tcttgccctg aatatggaat tgttggataa tatgccaggc ttatctgaaa actactaagt
     2281 gccagatatt catttttaat aatcattcca actccaaaaa cagaatacat acgacttctg
     2341 ttaaaaccat gaagttggtt accgatgaag cctgctgatg caatcagata cggtccgaac
     2401 ttaaatccca aaaagtcata aggcgcatag gtttgtgtct gcagagagat aaaaattcgc
     2461 ttcgtgccag aagtgcggct atcgaaacct ctcatctgat cttcgagcga aacaaattca
     2521 ttggtgattc tgtcaatacc caatgttgca tttgctctca ggaattgcct aagcctccac
     2581 ttccctattg ccatttgccg tgtaaaataa acggtctgta tttcaaatgc tgaatttctg
     2641 gcattaccgt tataaaaaaa tgttccgtag cgaaccaaac tgctcagata accaaaacta
     2701 aaataatctc cgaatgctgc cattgcacct gcataccaat tacctgatga agccctttct
     2761 ctatagccca ccgttaatcc ataggtgcga ccaacaggaa tatcttcttt ctgtccatat
     2821 ttaaagacat acctttccgt aatgtactct cttgaagaaa atccgattcc tgcaagatag
     2881 gtgttctgat tttttaatag acctaacgaa tcaaatcctg ccgaaggaaa agaaagatac
     2941 tgaacgctgc tgcctcttaa tgcagcaatc aatcgtgtgt tgcgctgata caccttatta
     3001 ccacttaaca aacgcatgga ataacccgac caggcatcta taaagttata tttatatcgt
     3061 tcggagtaat tttctgatgg ctcaccatat acggccttta cgaagtgttg ttctaataat
     3121 attccacctg catattttgt gagtggcgaa taaaatggcc tttcaactcc aattgacttc
     3181 acatcgcctt ctctttcgtc caaatggtaa agtatttttg catctgcatg ggttcccagc
     3241 aaattcggaa cctcataact gctttcaatg atgtcggcat ttttattgta ataccaccga
     3301 taactgttct caaaagtatt tcctaagcct gcaaagtttc tttctgttac actgattttt
     3361 gtattggttt cactcatgcc tccgccaatt aaaatactcc agttatccag cacacgtact
     3421 tcaaggtcta tggtttggtt tagctcattt ttatgaggaa taataatagc atcacgtata
     3481 taaggttgtg cacgaatcaa tcgttccgac tctgaaacta acaatgagtc aaactcatca
     3541 cctgtttcaa taatcattaa ttttctgatt tgccggttgc ttgttttatt atgcaaataa
     3601 tttccggcat tgataactga tggctgtttt ttaatggatg aatctgctaa agaataacca
     3661 aacggatcga atactgtaat gctaatgttt ccgactggca gacattgaaa ttccgaaaaa
     3721 ttaatttctt gtacaacagg ttttttctta acctttacgg cttttgcagt aacaggccgg
     3781 aaaatcattt tgtaaatact acgaagtagt ttactcgatt ttgaaacctg ctcaactccc
     3841 tgataaaaat caacagattt attttttgtt gtatctgttt gtgctattgc tgaaccacag
     3901 aaccaaaata aaatcagaaa agaaacaata ctgcggttca ttattttcat tgatacaaaa
     3961 gtaaattgcg tatgcaatta tgatttacga aaataacaat aagccccgaa ctaaaaagta
     4021 taaaaactat ttcagctttg ccagactaac ctttatacgg gcaatagctt ctgtgagcag
     4081 cttatcactt gtagcataag agaaacgaat acattgattg tcaccaaaag cttcacctgt
     4141 aactaaagca accttggcat ccttcagcag atacatacac atatcttcag gattattaat
     4201 tacagtactg ccatctgttt ttccaaaata ataacttatg tccggaaaaa cataaaatgc
     4261 accttcaggc gtattggttt tccatccggg aatatctttc atcaggttca aaaccaagtc
     4321 gcgtctttta cgaaaagttt ctaccatcat atctctgtcg ggtgtgctaa tgcttatagc
     4381 tgcctttgct gccttttgtg caatactact tgctgcagaa gtaaactgtc cctgcatttt
     4441 atcacatgca gttgccacat cctgtggtgc tgccatatag ccaattcgcc atcctgtcat
     4501 tgcatagccc ttacttacac cattgattat gatgacctga ttttttaatt cttcaaatga
     4561 tgcaatgctg tgatgcttac caacaaaatt gatgtactca taaatctcat cactcagcac
     4621 aaaaatattt tcatgttttg caaaaactgc tgcaagtgct tttaactcat catgcgaata
     4681 aacagtacct gtaggattac agggtgataa aaaaataaac atccttgttt ttgatgtaat
     4741 tgctttttca acctgctgtg gcgtaacttt aaaatcagaa tcaattgtag tattcagata
     4801 aataggtaca cctcctgcta attgaataat ctgcgaatag ctaacccaat aaggtgtagg
     4861 cactaacact tcatcacccg gattaacaca acataatact gcattggcaa tacattgctt
     4921 agctcccgtg ctgacaacta tctgtgatgc tttataatcg agattgtttt cgcgttttaa
     4981 cttctgcgaa atggcttcgc gcacatcagc ataacccgaa atgggtggat acttcgtaaa
     5041 cccgtcatca atagcttttt ttgctgcatc gccaatatat ttttgtgtat cnnnnnnnnn
     5101 naaacagagg ctgataatat tatgtccctg atcctgtagc tcgcggctaa ggcgtgccat
     5161 ggcaatggtt tgcgattcat gtaatcgctc tatacggtct gctaattttc gcatgtctga
     5221 tgcattcaat aatagggcgc aaagctaaca atacttcatc acagaaaata aaacaaaaag
     5281 caggtaatta accatgcttt gtgtattacc ggaaagttat ttacttgatt acaccaagtt
     5341 tttttccgac tttaataaaa gcattcaatg ctttgtcaat atgatggcgt tcatgccctg
     5401 cagaaagctg tactcttatg cgtgcatttc ctttggcaac aacaggataa aagaatccgg
     5461 taacatagat gccttcgtct aataacatag ctgcaaactg ttgtgctaac tgtgcttcgt
     5521 aaagcataat gggtacaata ggatgctgac cgggtttaat atcaaaacct gcttcggaaa
     5581 tttgtttacg gaaatattgt gtgttgtccc aaagtttatc gcgtagtgtt gttgtctgac
     5641 taagcatatc aagtacagca atggaagcac ctacaattga aggtgccaat gagttggaaa
     5701 acaaataggg tcttgaacgc tgacgcagca tttcaataat ttcttttcgt ccggatgtga
     5761 atccacccat agcaccaccc aatgccttgc ctaatgttcc ggtgataata tctatgcgtc
     5821 ccatcacacc atgatgttca tgtgtgccac ggcctgtctt tcccataaat cccgatgcat
     5881 gacattcgtc tatcatgatt aatgcctgat agcggtcggc caaatcacag attttatcta
     5941 actgtgcaat ggtgccatcc atactgaaaa cgccatcagt aacaatcata cgatgacgtt
     6001 tattcatagt agcttttaat ttctcttcaa gatctgccat atcattatgt ttgtaacgat
     6061 gacggtcggc cttgcacaat ctgacaccgt caataataga tgcatgattc agttcgtcag
     6121 agataatggc atcctgctca ttgagcaaag gctcaaacac tccaccgtta gcatcgaagg
     6181 ctgcagcata aagtatagtg tcttctgttt caagaaatga tgcaatcttt gattcaagtg
     6241 ttttatgaat atcctgtgtt ccacaaataa accgcacact tgacatgcca tagccatgtg
     6301 agttgattgc agcatgtgca gcctcaacta cttttggatg cgatgataaa ccgagataat
     6361 tattggcaca aaaatttata acttcttttc cgttggctgt aatatcggca gcctgtggtg
     6421 tagaaataat tctttcttgc ttaaacaatc ctgctgactt gatttcttca agctcctttt
     6481 ccagcacggg ctttaatgtg ttgtacatat catgaaaatt ttatgcaaag taataaaata
     6541 gaattcattt gaacctaatg ctgcaaatat ctaaacgagg gtattttcct actttagccg
     6601 catgagaatt gtgcgaatag tctttggtaa tgtgtggcgg ttctggtttt tctttgaagc
     6661 ggttgttacc ttttttctgt tgtttccgtt tttgctttta gcattgcagg gcaaagcacc
     6721 ctat
//
LOCUS       DAORQT010000081         6664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1804_length_6664_cov_15.078227, whole genome shotgun sequence.
ACCESSION   DAORQT010000081 DAORQT010000000
VERSION     DAORQT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1804_length_6664_cov_15.078227"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PK134_04260"
     CDS             <1..604
                     /locus_tag="PK134_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRB38092.1"
                     /translation="RDSRINRIFEGTNEINRLLTVDMILKRAMKGKIDLMTPAMNIQK
                     ELMAIPDFSEPDMSLFAQEKKFISNYKKALLAVAGSAVQKLMMTLAKEQEILMNIADM
                     AIAIYISESILLRTEKLVNLKGEENCSVQLDMMRVYVNDAADRIALAGKEAINSFAEG
                     DEQRMLLMGLKRFTKVPSFNVKEARRRIAARLIEKNEYCF"
     gene            627..1649
                     /locus_tag="PK134_04265"
     CDS             627..1649
                     /locus_tag="PK134_04265"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRB38093.1"
                     /translation="MIRKVFVPALILILLVSGFYYRHWLVYGFNQLKGQLNIIYNAQP
                     IGEVIENPLTPDSVKTKLILIRAIKKYAVDSLGLSETNNYETYYDQQGKPALWVLTAA
                     PEFKMVPYKWKFPFLGSLSYKGFFQKQKGVDELMLFRKNGFDADLSTVSAWSTLGWFG
                     DPVMSSMMRRNIGSLAELIIHEMTHTTLYIKGNVEFNENLATFIGEIGAEKFLTHKFK
                     DDSIVMTNYINHRNDNRIFSEYVIASVSKLDSLYSDKAFISLTYPKKLRLKYQKIASI
                     VLHIPKLPLKNDQRFEWNLQNKKLPDNTWFLSYSDYHALQPEINRVFRDSCNSSIQEL
                     IKYYKR"
     gene            complement(1662..2573)
                     /locus_tag="PK134_04270"
     CDS             complement(1662..2573)
                     /locus_tag="PK134_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38094.1"
                     /translation="MKSNKLFIYLTAIMLITVVACKKDRVPQDDYQNMDSFYSDHEEE
                     EQEYTIDTNGTCPLVCKKGTKICVNGEMFELNGGGQVTYPFQLKVIELYSIKDMILRR
                     IPSVASGNPLETSAEIRVRAFKNGNEIFLKPGKKYFLETAALPSINSAMQSYYGYSSG
                     NSANNWTNTISNFIPGFVDTLSSISPTSNSYVTTPATTGWFSPALLHQATSTTTITCS
                     VTGTNTQNLQVYVSFKGFKGVMQIQNLSGTNFPVGENVTLIVFGKKQNNQYVLDQQSF
                     TIAANQQIIFNLQDVSENDILNALAAL"
     gene            complement(2588..3094)
                     /locus_tag="PK134_04275"
     CDS             complement(2588..3094)
                     /locus_tag="PK134_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38095.1"
                     /translation="MFIAFNASAQRKVRKPIGYGASAIYNFQAEGIGADLRVRIPTPV
                     NNLYVVPEISYYPPFNRYHELYAGGALQYDILRIRSYNFYLLGAAYYNFWINADDYAP
                     EGKKKHNFAPEAGGGLVRNRGCWRPFIENRYDFKWKEDNLRIGIYYYPGQCRKNTRRE
                     KCPTVKGI"
     gene            complement(3219..3848)
                     /locus_tag="PK134_04280"
     CDS             complement(3219..3848)
                     /locus_tag="PK134_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRB38096.1"
                     /translation="MKNVLVVGAHPDDLEWMAGGTVAKIISEGGKVHLITLTNGTWKD
                     ASGNYYRDKDIAISEAKKAASVLGYSIEHLDEPCLDVNFRDELVVQVLNRIDKINADT
                     IICPWIDDLHHDHEMTARIAVSASRRVPRVLMGMCNWYVFRTPFSPNFFVDISDTYSK
                     KMAALECYESEMKRIGSTWRKYHDNITSNYGLMVNVERAEGFISYKYKL"
     gene            complement(3882..4853)
                     /locus_tag="PK134_04285"
     CDS             complement(3882..4853)
                     /locus_tag="PK134_04285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB38097.1"
                     /translation="MAENYHYHSYTPSDLDEILALFSLSFNGLKLSSEYYKWRVLNTP
                     VNKNLIDLFKNDNGKIISHYAVCPVKSFYKGETSLSELSLITMTHPEFKSLGLFPKLA
                     AKLYERSFKEENIQLVYGFPNTNSHYSFVKKLAWNDVYMIPTMKKTFTTAVQGDKTYT
                     EIFNADQRFDQLWEQHTQKADRFYANVRNSTFINWRFFNRPDKKYRLFAFENNSNIQG
                     YFVIKEYANASTKEIDVVDFLFTHDFDTSVFFNSIAQLATELDAKTANMWIPYNTPLY
                     NLAEKSGYLPKEHITYLGYKPFNPGKNLDELRIPTNWYLTMCDSDVY"
     gene            complement(4865..5569)
                     /locus_tag="PK134_04290"
     CDS             complement(4865..5569)
                     /locus_tag="PK134_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38098.1"
                     /translation="MTENCSIVIPVFNEEEILEKRIHLLVEDLKKRFKDFEIVLTENG
                     SVDNTKSIIRTLSEKYDMVVAEIDDEKADYGQALINGINRARFEYITIFELDYLDMVF
                     LQKSNELLSSYDLVIGSKKLSGDLDRRPFKRKLFTWLYNTGLKVAFSYKLTETHGLKS
                     FKKSKLAPITNGCVTRHAVWPSEFCIRASRDKNLKVTEIGLSMPIEEIRTTRIKAIKR
                     LKKTIDDILLLRRSIN"
     gene            complement(5566..>6664)
                     /locus_tag="PK134_04295"
     CDS             complement(5566..>6664)
                     /locus_tag="PK134_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38099.1"
                     /translation="RELVFDKLGLEVENHRRKAHIYYDKTIIPYPFELNLLNLSEQTK
                     KDCIEGLDEVKSYNKEEEAEIRKGSYRDYALKCFGKGIANHYLLPYNRKIWDTEPSEM
                     TCEFMSHIITADKEQIRKNAFEDNTDSFGYNVEFYYPIHKGIQDLADIFARQLTNIKF
                     NTSAIFFNTRNKTIEFSDGSIVKYNKAVSTVPLQTIILNSDRDDLKALAKELVFTSVY
                     TVNLVIRGTLPDTHWMYFPDKDLSFYRISFPKNYFKKSTPADEQIIAVEVGSRNHNLS
                     IEEIERKVVEEIKQMPIFKIDELVFVHSIKIPVAYCIYEPKRPAIINKLMSELEEAGI
                     YQSGRYGKWEYAGMQDAIMDGKVLSEKLKSL"
BASE COUNT         2067 a         1143 c         1155 g         2299 t
ORIGIN      
        1 tagagattca cgcatcaaca gaatttttga aggaacaaat gaaattaaca gactccttac
       61 tgttgatatg attctaaaac gtgccatgaa aggtaagatt gacttaatga caccggcaat
      121 gaatattcag aaagaactta tggcaattcc tgattttagt gagcctgata tgtcgctatt
      181 cgcacaagag aaaaagttta tttctaacta taaaaaagcg ttacttgcag ttgcaggttc
      241 tgctgtacaa aaattgatga tgacacttgc taaagagcag gagattctga tgaacattgc
      301 agatatggca attgcaattt atatttcaga gtcaatttta ttgaggacag agaaacttgt
      361 taatttaaaa ggtgaagaaa attgcagtgt tcagcttgat atgatgcggg tttatgtaaa
      421 tgatgcagca gatagaattg cattggcagg aaaagaagca attaattctt ttgcagaagg
      481 tgacgaacag agaatgttgc tcatgggatt aaaacgcttt accaaggttc cgtcattcaa
      541 tgttaaagaa gcaagaagaa gaattgcagc acgcttaatt gaaaagaacg aatactgctt
      601 ctgatttgta gagcagttct aagctgatga tcaggaaggt ttttgttccg gcactcattt
      661 taattttatt agtttcagga ttttattacc gccattggtt ggtttatggg tttaatcaac
      721 taaagggtca gctaaatatt atttacaacg cacagcctat tggcgaagta atagagaatc
      781 cattaactcc cgattcagtt aaaacaaaac tgatattaat ccgtgcaata aaaaaatatg
      841 ctgttgattc tttaggatta tcagaaacta acaactacga aacttattat gatcaacaag
      901 ggaaacctgc attgtgggtg cttaccgctg ctcctgaatt taaaatggta ccatataaat
      961 ggaagttccc atttcttggg tctttatcat ataaaggatt ttttcagaaa caaaaaggag
     1021 ttgatgaatt gatgttgttc aggaaaaacg gctttgatgc agatttgtca acagtatcag
     1081 catggagtac ccttggctgg tttggtgatc ctgtaatgtc ttcaatgatg cgaaggaata
     1141 ttggctcgct tgcagaattg attatccatg agatgactca tacaacgctt tacattaaag
     1201 gaaatgttga attcaatgaa aaccttgcga cttttattgg tgaaattggt gctgaaaaat
     1261 ttctgactca taaatttaaa gatgacagca ttgttatgac aaattacatc aatcatcgca
     1321 atgacaacag aatattttca gaatatgtaa ttgcttcagt atctaaattg gactcattgt
     1381 atagtgataa agcttttata tcattaacct atccgaagaa actacgtctg aaatatcaaa
     1441 aaatagcatc aattgttttg catataccga aattaccatt gaaaaatgat caaaggtttg
     1501 agtggaatct gcaaaataaa aaactgcccg acaacacctg gtttctatct tattctgact
     1561 accatgcttt gcagcctgaa attaatcggg tgtttagaga ttcctgcaat agttcaattc
     1621 aggaattgat aaaatactat aagagataag gcgtaattta cttacaatgc tgcaagagca
     1681 ttcagaatgt cattttccga aacatcctga agattaaaaa taatttgctg attggcagca
     1741 attgtaaaac tctgctgatc taaaacgtat tgattgtttt gttttttacc gaatactatc
     1801 aatgttacgt tttcgccaac aggaaagttt gtgccactca ggttttgaat ttgcataaca
     1861 cctttaaaac ctttgaatga aacatatacc tgtaagtttt gcgtatttgt tccggtgaca
     1921 ctacatgtaa tagttgttgt tgatgttgcc tgatgtaata atgcaggaga aaaccaacct
     1981 gttgtggcag gtgttgttac ataactgtta gaagttggag aaatactcga tagtgtatca
     2041 acaaaaccag gaataaaatt agaaatagta ttggtccagt tattagcact attaccgctg
     2101 ctataaccgt aataggattg catggctgaa tttatagatg gcaatgcagc tgtttcaaga
     2161 aaatattttt tgccgggttt gagaaaaatt tcatttccgt ttttaaatgc tctcactctt
     2221 atttctgcac ttgtttcaag tggatttcct gaagccacag aaggaattct tcttaaaatc
     2281 atatctttaa tagaatacaa ctctattact tttagctgaa aaggataagt aacctggcct
     2341 ccaccattta actcaaacat ttctccgttg acacaaattt tagttccttt cttacaaact
     2401 aatggacaag ttccattggt gtcaatggta tattcctgtt cttcttcttc atggtcgctg
     2461 tagaatgaat ccatgttttg ataatcatcc tgtggcacac ggtctttttt gcatgcaact
     2521 actgtaataa gcattattgc agtaagataa atgaatagct tgttcgattt catattacaa
     2581 agtttgatta aattccttta actgttggac atttttctct tcttgtattt tttctacact
     2641 gtccgggata ataataaata cctattcgca aattatcttc tttccattta aagtcatagc
     2701 ggttttcaat aaatggacgc caacatcctc tgtttcgcac caagccacca ccggcttcgg
     2761 gagcaaaatt gtgtttcttt tttccttcag gagcatagtc atctgcattt atccagaaat
     2821 tgtaataggc agcaccaagt aaataaaaat tataacttct gattcttaat atatcatatt
     2881 gcaaagcccc accggcatac aattcatgat aacgattaaa aggagggtaa tatgaaattt
     2941 caggaactac ataaaggtta tttaccggtg ttggaattct gacacgcaaa tctgcaccaa
     3001 taccttcggc ctgaaaatta taaattgcac tggcaccgta gcctataggt ttacgtactt
     3061 tacgttgtgc tgaagcattg aacgcaatga acattgcaaa caaaataatg gatatatgcc
     3121 tgattttaaa cattgaaata ttttgggatt aaagatgtta actttatcta ttcccaaaac
     3181 ggcattactg gaaaattatt atttgattat aaacagaact acaatttgta tttgtagctg
     3241 ataaaacctt ctgcgcgctc tacattcacc atcagaccat aatttgaagt gatgttgtcg
     3301 tgatattttc tccaagtgct cccaatgcgt ttcatttctg attcatagca ttctaatgct
     3361 gccatctttt tagaatatgt gtcgctgatg tcaacaaaaa agttaggact aaatggtgta
     3421 cgaaacacgt accagttgca catgcccatg agtacacgtg gcacacgcct gcttgcactc
     3481 actgcaatac gtgcagtcat ttcatgatcg tgatgtaaat cgtcaatcca tggacatatt
     3541 atcgtatctg catttatttt gtcaatgcgg tttaatacct gaactactaa ttcatctctg
     3601 aaatttacat caagacaagg ttcatctaaa tgttctattg agtagcctaa aacagaagca
     3661 gcttttttag cttcactgat agcaatatct ttatcacggt agtaatttcc ggaagcatct
     3721 ttccatgtac cattggttaa agttattaaa tggactttac ctccctctga aatgattttg
     3781 gcaacagtac caccggccat ccattccaaa tcatcaggat gtgcacctac tactaaaaca
     3841 tttttcatgt tgtcattgat tttaaattca atagtaacct atcagtaaac atcagaatca
     3901 cacattgtca aataccagtt tgttggtatt cttaactcat ctaaattttt tcctgggttg
     3961 aatggtttat atcctaagta agttatatgt tctttcggca aatagcctga tttttcagca
     4021 agattataca atggagtatt gtagggaatc cacatgtttg ctgttttagc atctaattct
     4081 gttgctaatt gtgctatgga attaaagaat actgatgtgt caaaatcatg tgtaaaaaga
     4141 aaatcaacaa catcaatttc ttttgttgat gcatttgcat attccttaat tacaaaataa
     4201 ccttgtatat tactgttgtt ttcaaatgca aataagcgat attttttatc agggcgatta
     4261 aaaaaacgcc agtttataaa tgtgctgttt ctgacgttcg cataaaaacg atctgccttc
     4321 tgtgtgtgtt gctcccatag ctgatcaaat cgttggtcgg cattaaaaat ctctgtataa
     4381 gttttatcac cttgaacagc agttgtaaat gtttttttca ttgttggaat catatataca
     4441 tcattccaag ctaatttttt tacaaagctg taatgcgagt tggtgtttgg aaacccataa
     4501 accaattgaa tattctcttc cttgaaggag cgctcgtaaa gttttgctgc aagttttgga
     4561 aacagtccta atgatttaaa ttcaggatgt gtcattgtga tgagcgaaag ttcacttagt
     4621 gaagtttcgc ctttgtaaaa agattttacc ggacacactg cataatgact tattatttta
     4681 ccattgtcat ttttaaataa atcaatcagg tttttattta caggagtatt tagaacacgc
     4741 catttataat attctgatga gagttttagt ccgttaaatg aaagagaaaa taaggcaagt
     4801 atttcatcaa gatctgatgg agtgtaactg tgataatgat aattttctgc catagcgtat
     4861 tttattagtt aatagatctg cgtaacagca aaatatcgtc aatagttttc tttaatcttt
     4921 ttattgcttt aatacgggta gttctgattt cttcaatcgg cattgataag ccaatctctg
     4981 ttacttttaa atttttatca cgtgaagctc ttatgcaaaa ttcacttggc caaacagcat
     5041 gtcgtgttac acagccatta gtgattggag ctaatttact tttcttaaaa cttttcagtc
     5101 catgagtttc tgttaacttg tagctaaatg caactttaag ccctgtattg tagagccagg
     5161 taaaaagttt tcttttaaat ggtcttctgt caagatcgcc agaaagtttt tttgagccaa
     5221 taacaagatc gtatgagcta agcaactcat ttgatttttg taaaaagacc atatctaaat
     5281 agtcaagctc aaatatggtg atgtactcaa aacgtgcccg attaatgccg ttaatcaaag
     5341 cctgaccata gtctgctttt tcgtcatcaa tttctgcaac aaccatatca tacttttcag
     5401 atagtgttct gatgatagat ttggtgttgt caacgcttcc gttttctgtt agtacaattt
     5461 caaaatcttt aaaacgcttt tttaaatcct caacgagcag gtgtattctt ttttcaagaa
     5521 tttcttcttc gttaaatacc ggtattacta ttgaacaatt ttctgtcata atgattttaa
     5581 tttttccgac aatactttgc cgtccataat tgcatcctgc atgcctgcat attcccattt
     5641 tccatagcga cctgactgat aaattcctgc ttcttccagt tcactcatca gtttgttgat
     5701 tatggcaggg cgttttggtt cgtatataca ataggccaca ggtattttaa tcgagtgaac
     5761 aaataccaat tcgtcaattt tgaatattgg catttgttta atctcttcaa caactttgcg
     5821 ttctatttct tcaatactca ggttatgatt gcgactgcca acttcaacag caataatttg
     5881 ttcgtcagca ggtgttgatt ttttgaaata gtttttaggg aagcttattc tgtagaacga
     5941 aagatcttta tcggggaaat acatccaatg tgtgtcaggc agtgttcctc tgataactaa
     6001 atttactgta taaacagagg tgaaaaccaa ttctttggct aatgctttca agtcatctct
     6061 gtcactgttt aatatgatag tttgtagggg aacagtagaa acagctttgt tatattttac
     6121 aatacttcca tcagaaaatt caattgtttt attccgtgta ttaaaaaata ttgcagaagt
     6181 attgaattta atattagtga gttgtctagc aaaaatgtct gctaaatcct gaataccttt
     6241 atgtatagga taatagaatt ctacattata gccaaaactg tctgtgttgt cttcgaatgc
     6301 attttttctg atttgctctt tgtctgcagt aataatatgc gacataaact cacatgtcat
     6361 ttcagaaggt tcggtgtccc aaatttttct gttgtaaggt agaaggtaat gattggcaat
     6421 accttttccg aagcatttca atgcataatc tctgtaagag ccttttctga tttctgcttc
     6481 ctcttcctta ttatatgatt ttacttcgtc aagtccttca atacaatctt ttttggtttg
     6541 ctcactcaga tttaatagat tgagctcaaa agggtagggg atgatggttt tatcgtaata
     6601 gatgtgagct tttctacgat gattttcaac ttcaagaccc aatttgtcaa atactaactc
     6661 tctt
//
LOCUS       DAORQT010000082         6598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1843_length_6598_cov_10.225126, whole genome shotgun sequence.
ACCESSION   DAORQT010000082 DAORQT010000000
VERSION     DAORQT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1843_length_6598_cov_10.225126"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..4961
                     /locus_tag="PK134_04300"
     CDS             72..4961
                     /locus_tag="PK134_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HRB38100.1"
                     /translation="MLNEFKKSQPDLLNKKNKKGEEFKIPYDWTIYYLRKKALTQKLA
                     KEELAWLILNFNQKRGYYQLRGEEEEENQNKLVEFHSLKVVEVKAEIKYILTLENNET
                     YYRLSETPLLDWKDTNRDFVVTTYLETDGTVKKDKDGKEKRSLFLKDDLVFIKKKTTK
                     ITKSTKNKNETVEEKSIKIIDVKEEKQNEIWYSLILENGWIYRRQSKVSLEDWKGKVR
                     EFIVTTDLNDDSSIKTDDEGKESRSFRAPKEDDWTLIKKKTEDDLSKYIGEYKTKNGI
                     DGTVGTYIYDNLLRKPNQKIKGKLVRTIERKFYKYELREILKKQIVLQPELFSDDLYN
                     DCVRELYKNNEAHQLQLNKRDFIHLFLDDIIFYQRPLRSQKSSIGNCSLEFRLHKINK
                     KDDKGNSIKNEFEKDENGKDIEVKEYLKVIPKSNPYYQEFRLWQWLYNLKIYKKEDDT
                     NITADFITSNDDKITLFDFLNSRKEIEQKPLIEFLVKQRELKNPANNNLNKKVQTALS
                     KKIKEETEKYRWNYVEDKKYPCNGTGTQIRTRLERVTNVSVSFLTREIEQHLWHIIYS
                     VTDKNEFEKALKSFSKRHQIDETSFVENFKNFPPFISEYGSLSEKAIKKLLPLLRIGK
                     YWNENAINDKIKKRANSISERLKSISKKEDIENISDDDIQKQILKSFYGLTDLTQGLQ
                     LYQASYLIYGRHSEASDLQSWNSVADLEKYLKEFKQHSLRNPIVEQVVTETLRVVKDI
                     WTHYGKGVENFFDEIHVELGREMKNTAEDRAVLTKQVTSNENTNMRIKLLLAELKNDT
                     TIEGVREYSPSQQEILKIYEDGVLNSNVEIPNDISEILKKFKESDIKKQPTKTEVNRY
                     KLWLEQKYRSPYTGQMIPLSKLFTEAYQIEHIIPQSRYFDDSFNNKVICEAAVNQLKD
                     KQLGLEFIKNHSGQIIDLGQNQSVTLFTEQQYTDFVKEHYDKNRSKRTKLLLDEIPDK
                     MIERQLNDTRYISKFISSVLSNIVRANKDDDGVNSKNLLPGNGKITSELRQDWGLNDV
                     WNDLILPRFERMNELSQSNHFTATNKEGHTIPAIPLELSKGFQKKRIDHRHHAMDALV
                     IACATRDHVNLLNNQSAKSDNSRYDLQRKLRKVEKWTDKEGKERDKFTDFYKPWSTFT
                     QDTKNELEKIVVSFKQNLRVINKASNHYEKIINGKKVLVKQEGSNWAIRKPMHKDTVS
                     GLVQLRKTKTVSLSVALENYNDIVDKTLRKHILSLINQNYDKKKLTKYFKDNENKWNN
                     RDISKLDIYYLENDNVASRVNLDISFNKERIEDTITDTGIQKILLKHLINYEGQTDEK
                     GKAIAPETLAFTPEGIDEMNKNIITLNNGKSHQPILKVRTYEPKGNKFTIGETGNKKD
                     KFVEAAKGTNLFFAIYEDENGNRSYETIPLNIVIERQKQGLCSVPEQNEKGHALHFHL
                     SPNDLVYVPQVDENLDAINFGELTIEQSNRIYKFTDGSGIMANFIPSKIAEVIFNINK
                     LKQESLKINYPIQNEIGVGSQGSKNERAISGEQIKSICIKLNIDRLGNITPSKKTNQK
                     STSSFVNEEHSIYYNKVTFNNLQQSDEKMFMHTANMNPEERMAYLQKLRETTQENISE
                     AEKRAILNQIKINPPDENT"
     gene            5026..5985
                     /gene="cas1"
                     /locus_tag="PK134_04305"
     CDS             5026..5985
                     /gene="cas1"
                     /locus_tag="PK134_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRB38101.1"
                     /translation="MIKRTLYFGNPAYLSLKNSQLMVRLPEVEKNQQLDVAFKQQAHA
                     TIPVEDIGIVLLDHQQITITHGLLDALMENNVAVITCNNTRHPAGLLLPLQGHTLQHE
                     RYSDQLSASEPLRKQLWQQTIIQKIKNQAAGLQLQNIDSTRLYPIYNNVKSGDSENAE
                     GVAAAFYWSNIFTKIPATIEHAPGNLFKRQRDGLPPNNYLNYGYAILRATMARSIVAA
                     GLLPTKGIHHHNRYNAYCLADDLMEPFRPFVDRIVIDIILKHGIIDDLTKAIKADLLT
                     IPVIDVMMDGENSPLTIATQRTATSLVKCFAGEQRKLIYPELL"
     gene            5982..6320
                     /gene="cas2"
                     /locus_tag="PK134_04310"
     CDS             5982..6320
                     /gene="cas2"
                     /locus_tag="PK134_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRB38102.1"
                     /translation="MNFERLNAYRTMWTIVMYDLPTETKKQRKLAAKFRKDLLQDGFQ
                     MFQFSMYLRHSSSSENADVHRNRVKKSLPTEGKVGILQITDKQFGQMEIFYGKKAHDK
                     PQTSQQLELF"
BASE COUNT         2635 a         1032 c         1182 g         1749 t
ORIGIN      
        1 ggaagaaaaa tgggaaaaaa ccgaatggaa aagataagtt tgaatttgct tttcaatcaa
       61 catttgaaga aatgttgaac gagttcaaaa aatcacaacc cgacttattg aataagaaaa
      121 acaaaaaagg tgaagaattt aaaattcctt acgattggac gatttattat ttgcgaaaaa
      181 aagcactcac acaaaaatta gcaaaagaag aattggcttg gttgatttta aatttcaatc
      241 aaaaacgtgg ttactatcag ttgcgtggtg aagaagagga agaaaatcaa aataagttgg
      301 tagaatttca ttcgttgaaa gtagtagaag taaaagcgga aataaaatac atcttaactt
      361 tggaaaacaa tgaaacctac taccgtttaa gcgaaacgcc tctattggat tggaaagata
      421 caaaccgcga cttcgttgta acaacctatt tggaaactga cggaacagta aaaaaagata
      481 aagacggaaa agaaaaacgt agtttgtttt tgaaagatga tttagttttt atcaaaaaga
      541 aaacaacaaa aattactaaa tcaacaaaaa ataaaaatga aacggttgaa gaaaaatcaa
      601 taaagattat tgatgtaaaa gaagaaaaac aaaatgaaat ttggtattcg ttaatattag
      661 aaaacggctg gatttatcga agacaaagta aagtttcatt agaggattgg aaaggcaaag
      721 tgcgtgaatt tattgttacc accgatttaa acgatgacag ttcaataaaa acagatgacg
      781 aaggaaaaga aagtcgcagt ttccgtgcac cgaaagaaga tgattggact ttaataaaaa
      841 agaaaaccga agatgatcta agtaaataca ttggagaata taaaacaaaa aatggaattg
      901 acggaactgt cggcacatac atttacgata atttgcttcg aaagcccaat caaaaaatta
      961 aagggaaatt agttcgtacg attgaacgca agttctacaa atatgaatta agagaaattc
     1021 ttaaaaagca aattgtactg cagcccgaat tgttttcaga tgacttgtat aatgattgtg
     1081 taagagagtt gtataaaaac aatgaggcac atcaactgca actaaataaa cgagatttta
     1141 ttcatttgtt tttagatgac attatttttt atcaacgccc tttgcgaagc caaaaatcaa
     1201 gtattggtaa ttgttcgttg gaatttcgat tacataaaat caataagaaa gacgataagg
     1261 gaaattcaat aaaaaatgag ttcgaaaaag acgaaaatgg aaaagatatt gaagtaaaag
     1321 aatacttaaa agtcattccg aaatccaatc cgtactatca ggagttccgc ttgtggcaat
     1381 ggttgtacaa cctgaaaata tacaagaaag aagatgatac aaatattacg gctgatttta
     1441 ttacaagcaa tgacgataaa attactcttt ttgatttttt gaacagcaga aaagaaattg
     1501 agcaaaagcc tttgattgaa tttttggtta agcaacggga actgaaaaat cctgcaaaca
     1561 ataatttaaa caaaaaagta caaacagcac taagcaaaaa gataaaagaa gaaacagaaa
     1621 aataccgatg gaattatgtt gaggataaaa aatatccgtg caacggaaca ggaacgcaaa
     1681 ttcgcacacg attagaaaga gtaacaaacg tttctgttag tttccttaca cgcgaaattg
     1741 agcaacatct ttggcacatc atttattccg taactgataa aaacgaattt gaaaaagctt
     1801 taaaatcatt ttcgaaaaga catcaaatag atgaaacttc gtttgtagaa aatttcaaaa
     1861 atttcccacc atttataagt gaatatggta gtctttcaga aaaagcaata aagaaattat
     1921 tgcctttgct tcgcataggt aaatattgga acgagaatgc aataaacgat aaaattaaaa
     1981 aaagagccaa ttccataagt gaacgattaa aatcaatttc aaaaaaagaa gatatagaaa
     2041 acatatctga cgatgatatt caaaaacaaa ttttgaaaag tttttacggt ttaactgatt
     2101 taacacaagg cttacaatta tatcaagcaa gttatttgat ttatggaaga cattctgaag
     2161 caagtgatct acagagctgg aactcggttg ccgatttgga aaagtatctc aaagaattta
     2221 aacagcattc gttacgaaac ccgattgtag agcaagttgt taccgaaaca cttcgtgtgg
     2281 taaaagatat ttggacacat tacggaaaag gtgtagaaaa tttctttgat gaaattcacg
     2341 ttgagttagg gcgtgaaatg aaaaatacag ctgaagaccg cgcagtcctc accaaacaag
     2401 ttaccagtaa tgaaaatacc aacatgcgta ttaagttgtt attggctgag ttaaaaaacg
     2461 acacaactat tgagggcgtt agagaatatt cgccctccca gcaagagatt ttgaagattt
     2521 acgaggacgg agtattaaat tctaatgttg aaattccgaa tgatatttct gaaatattaa
     2581 aaaaattcaa agaaagtgat attaaaaaac aaccaacaaa aacggaagtc aatcgttata
     2641 aattatggtt agaacaaaaa tatcgttcgc cttacacagg gcaaatgata ccgctttcta
     2701 aattgtttac cgaagcatat cagatagaac acatcattcc gcaaagccgt tattttgatg
     2761 atagtttcaa caacaaagta atttgtgagg cagccgtaaa ccaacttaaa gacaaacaac
     2821 ttggtttgga gtttattaaa aatcattcgg gacaaattat tgatttaggg caaaaccaaa
     2881 gtgtaacact ttttacagag cagcaataca ctgattttgt aaaggagcac tacgacaaaa
     2941 accgttcaaa acgaacaaaa ttgttgttgg acgaaattcc cgacaaaatg atagaacgcc
     3001 aattgaatga tacacgctac atcagcaagt ttatttcatc ggtgttatca aatattgttc
     3061 gtgccaacaa agacgatgac ggagtaaact ctaaaaatct tttgcccgga aacggaaaaa
     3121 tcacttccga actgcgacaa gattgggggt tgaatgatgt ttggaatgat ttaattctgc
     3181 ctcgttttga gagaatgaat gaattgtcac aaagtaacca ttttacggca accaataaag
     3241 aaggacacac cattcctgct attccattgg aactgtctaa aggtttccaa aaaaagcgta
     3301 ttgaccaccg tcatcatgca atggatgctt tggtcatagc gtgtgcaact cgtgaccatg
     3361 tcaatttatt gaacaatcaa tctgccaaat cagacaatag tcgctatgat ttgcaacgta
     3421 aactgcgaaa agtagaaaaa tggactgaca aagaaggaaa agaaagagat aaattcactg
     3481 atttctacaa accttggtca acatttacac aagatactaa aaacgaatta gagaaaattg
     3541 tcgtcagttt caagcaaaac ttaagggtaa ttaataaggc aagcaaccac tacgagaaaa
     3601 ttatcaacgg taaaaaagtt ttggtaaagc aagaaggatc gaattgggcg ataagaaaac
     3661 ccatgcataa ggatactgtt tcgggactgg ttcaattaag gaaaacaaaa acagtttctt
     3721 taagtgtggc tttagagaat tataatgaca tcgttgacaa aactttacgt aagcatattt
     3781 tatctctaat taatcagaat tatgataaaa agaaattaac aaaatacttc aaagacaatg
     3841 aaaataaatg gaataataga gatatttcta aattagatat atattacttg gaaaatgaca
     3901 atgttgcatc cagagttaat ttagacatat catttaataa agaaagaatt gaagatacca
     3961 taacagatac tggtattcaa aaaatcttat tgaaacattt aatcaattac gaaggacaaa
     4021 cagatgaaaa aggaaaagca attgcacctg aaactttagc ttttacacca gaaggaattg
     4081 acgaaatgaa taaaaacatc attactttga ataacggtaa atctcaccaa ccaatcctaa
     4141 aagttagaac atatgagcct aaaggaaata agtttaccat aggcgaaacc ggaaacaaaa
     4201 aagataaatt tgtagaagct gccaaaggaa ccaacctttt ctttgccatc tatgaagatg
     4261 aaaatgggaa tagaagctat gaaaccattc cattaaacat tgtcattgaa agacaaaaac
     4321 aaggtttgtg ttccgttcct gaacagaatg aaaaaggaca tgctttacat ttccatttat
     4381 caccaaatga tttggtgtat gtgccccaag tagatgagaa tctggatgca attaactttg
     4441 gtgagttgac gatagagcaa tcgaatcgaa tttataaatt tactgatggt agtgggataa
     4501 tggcaaattt tataccttca aaaattgctg aagttatttt taatataaat aaattaaaac
     4561 aagaaagttt gaaaattaac taccctattc aaaatgaaat tggtgtagga tcacaaggct
     4621 caaaaaatga aagagctata tctggtgagc agattaaaag tatttgtatt aaactcaaca
     4681 tagaccgctt aggcaacatt actccttcaa aaaaaactaa tcagaaatca acatcaagtt
     4741 ttgtaaatga agaacattct atttactaca ataaagttac attcaacaac cttcaacaat
     4801 ccgatgaaaa aatgtttatg catacagcca acatgaatcc tgaagagcgt atggcgtatt
     4861 tgcaaaaact gcgtgagaca acacaagaaa atatttctga agcagaaaag agagccattt
     4921 taaatcagat aaaaataaat ccgccagatg aaaatacttg atttggaaca attgcaacaa
     4981 attcatcgtt taaaaaacaa gaaatcgtga tgagataaaa aacaaatgat taaacgtacg
     5041 ctctattttg gcaaccctgc atacctgagc ttaaagaaca gtcagctaat ggtgcgtttg
     5101 cccgaggtgg agaaaaacca acaacttgat gttgcgttta aacaacaggc acatgctacc
     5161 attcctgttg aagatattgg tattgtattg ctcgaccatc aacaaattac cataacgcat
     5221 ggattactcg atgcattaat ggagaacaat gttgccgtta taacatgcaa caatacccgg
     5281 catcctgcag gactgttgct gccactgcaa gggcacactt tacagcacga acgatacagt
     5341 gatcagcttt cggccagcga gcctttacgg aaacaacttt ggcagcaaac cattattcaa
     5401 aaaattaaaa atcaggcagc aggattacaa ttacaaaata ttgattccac acgcctctac
     5461 cctatctaca acaatgtaaa aagtggcgat agtgagaatg ccgaaggtgt tgcagcagcc
     5521 ttctactggt caaatatttt tacaaaaatt cctgctacaa tagaacatgc accgggtaat
     5581 ttattcaaac gacaacgtga tggtttgcca cccaacaatt atctgaatta tggttatgca
     5641 attcttagag caactatggc aagaagtatt gttgctgcag ggctcttacc aacaaaaggt
     5701 attcatcatc ataaccgtta caatgcctac tgccttgcag atgacctgat ggagcccttc
     5761 agaccttttg ttgacagaat tgttattgac attatcttaa aacatggtat aattgatgac
     5821 ttgacaaaag ctattaaagc agatttattg acaattcctg taatagatgt aatgatggat
     5881 ggagaaaaca gtccattaac aattgctaca cagcgaacag ctacttcatt agtaaaatgt
     5941 tttgccggtg aacaacgcaa actaatttac ccggagttgt tatgaatttt gaacgcctca
     6001 atgcctatcg cactatgtgg actattgtta tgtacgacct gcctactgaa actaaaaagc
     6061 aaagaaaatt agctgctaaa ttcagaaaag atttattgca ggatgggttt caaatgttcc
     6121 aattcagtat gtacttgcgg catagcagca gtagcgaaaa tgctgatgtg catcgaaaca
     6181 gagtaaaaaa atcattgcct actgaaggta aggttggaat tttgcaaatt acggataaac
     6241 aattcggtca gatggaaatt ttttatggca aaaaggcgca tgacaaacca caaacatcgc
     6301 aacaacttga gctattttaa agggtgattt aagtcaatcc gttaaatttc aggaataaaa
     6361 aatcccgctg tttgggcggg acttcaagtt cttaaactca tttttactat cctgaaactg
     6421 ttcttttaag ttgaatatct gttgtttagc caatagatat tgaaagaact ctgacaaaga
     6481 taaaagttga aagcaaatca caacatggaa ggtgaggatg attcaaaata agaaattgaa
     6541 aaaaatctga aaaagaaaaa atttcaaaga aaaacaaaac acaaaagata agaaaaaa
//
LOCUS       DAORQT010000083         6545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1874_length_6545_cov_20.158089, whole genome shotgun sequence.
ACCESSION   DAORQT010000083 DAORQT010000000
VERSION     DAORQT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_6545_cov_20.158089"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK134_04315"
     CDS             <1..606
                     /locus_tag="PK134_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HRB38103.1"
                     /translation="AETVLKKEMDDAIKNNKPVYFLSFHVDYWNKYGWKDPYSSIKYS
                     RRQNNYVSATGSNEVFTPQVFVNGNTGFVGSDSKRLNSEIEKGLKSVAKQKLTVTVLQ
                     TLNDTLHLNYTASKADANANIIVAVVQKSVTTKITKGENIGRTLVQDNVVRLYEVYPV
                     ATKGTLLLKTNKLALNENFQLIAFIQNKQSKKISAATALTL"
     gene            complement(621..1799)
                     /locus_tag="PK134_04320"
     CDS             complement(621..1799)
                     /locus_tag="PK134_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRB38104.1"
                     /translation="MSKDRFQAPDYFLMDELLTDEHKLIRDSVREWVKKEVSPIIEDA
                     CQKCEFPKHLIKGLAEVGAFGPYIPAEYGGGGLDQISYGLIMQELERGDSGIRSTASV
                     QSSLVMYPIYTYGSEAQRKKYLPKLASGEWMGCFGLTEPDHGSNPGGMVTNIKDKGDH
                     YLLNGAKMWISNAPFAQVAVVWAKDEEGVIRGLIVERGMKGFTTPETHGKWSLRASAT
                     GELVFDNVEVPKENLLPNVKGLKGPLGCLNSARYGIAWGALGAAMDCYDSALRYAKER
                     KQFGRPIGGFQLQQKKLAEMLTEITKGQLLVWRLGKLKNENRANPAQISMAKRNNVHT
                     ALWCAREARQIHGGMGITGEYPIMRHMMNLESVITYEGTHDIHLLITGMDITGENAFT
                     "
     gene            complement(1891..5553)
                     /gene="metH"
                     /locus_tag="PK134_04325"
     CDS             complement(1891..5553)
                     /gene="metH"
                     /locus_tag="PK134_04325"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRB38105.1"
                     /translation="MKNIRQELEKRILIIDGAMGTMIQRYKLSEEDYRGERFADWKSD
                     LKGNNDLLSLTKPEIIEAIHLEYLEAGADIIETNTFNAQKISLADYHMESLAYELNFE
                     SAKIAKKAVESFKKKHQSDKKFYVAGAVGPTNRTLSLSPDVNRPGFRAVSWDQMVDAY
                     TEQINGLVDGGADLLLVETIFDTLNAKAALVAIQNVMEQKKVDLPVMISGTITDASGR
                     TLSGQTVEAFLNSVSHVNLLSIGLNCALGAKEMRPYLEELSAKSPFYISCYPNAGLPN
                     QFGEYDETPHQMCVQIDDFLESGFVNIIGGCCGTTPEHIKHIAEHAKNHKPRAIPTIE
                     PYLRLSGLEAVTLRPDSNFMNVGERTNVTGSKKFLRLIKDNKYDEALSVAREQVEGGA
                     QAIDINMDEGMLDAVEAMTTFLNLIAAEPDISKVPVMIDSSKFEVLEAGLKCIQGKGI
                     VNSISLKGGEDQFIYEAKTVKKYGAAVIVMLFDENGQADSTPRRIEIAKRAYDILVNK
                     VNFPAQDVIFDPNIFPVATGLEEHRQNAISYFEASKWIKENLPLAKISGGVSNVSFSF
                     RGNDPIREAIHAVFLYYGIKNGMDMGIVNPSQLIVYDEIEKDLLALVEDVILDRREDA
                     TEKLLAKAEELKLSQTTTDKNVKKGNQSANAWRSEDLQSRITHSLIKGIDEFVDQDME
                     EARQQVSKALEVIEGHLMIGMGVVGDLFGQGKMFLPQVVRSARVMKKAVAYLTPFIEA
                     EKVKGQSKGKIVLATVKGDVHDIGKNIVGVVLACNNYEIVDLGVMVAADKILQAARDH
                     QADIIGVSGLITPSLDEMVHVAKEMEREKFTMPLLIGGATTSKVHTAVKIEPNYSGVV
                     VHVNDASRSVPVVSNLLSAEQKEKFIEDIKAENERTRSYHKAERSRGKFLSLKEAQQN
                     KYELNGAVVTEPKFKGVKVFSNYDLSLLTEYIDWTPFFIAWEMKGSYPKILSDPQRGQ
                     EAQKLFDDAQTMLQRIIKEKWLTANAVFGIFPAANKGDDIEVFDERRENKIATLHTIR
                     QQSERATGQKNIALADFIRPSDYIGAFAVSTGFGIEKWLKKFADDHDDYSSIMLKVLA
                     DRLAEAFAEHLHERVRKEFWAYTTDENLNRQDLIKEKYQGIRPAAGYPAQPDHTEKYT
                     LWKLLDVEKNTGITLTEHLAMFPTAAVSGLYFAHPESHYFAVGKIQRDQVEDYAKRKS
                     MTVEEAEQWLGSILGY"
     gene            complement(5592..6254)
                     /gene="mnmD"
                     /locus_tag="PK134_04330"
     CDS             complement(5592..6254)
                     /gene="mnmD"
                     /locus_tag="PK134_04330"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRB38106.1"
                     /translation="MDSIEIVKTKEGIDTLINTRINEHYHSLHGAMQESMHVFIRNGL
                     LALNKSLPDIKILEMGFGTGLNAILTYRECYVLQKPIHYTTIESSPLPMAIINKLNYF
                     EYFGKALLPMFQKMHDCNWFENVTFGDFVLHKINADMLELKLDGGYDLVYYDAFSPTH
                     QPELWSFEVLHKVYESCNRGAMLVTYCANGEVKRTLKKVGFAVEALPGPKGKREMIRA
                     CK"
     regulatory      complement(6337..6442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1893 a         1462 c         1082 g         2108 t
ORIGIN      
        1 gcagaaacag ttctgaaaaa agaaatggat gatgccatta agaataataa gcctgtttat
       61 tttttaagct ttcatgttga ctactggaat aaatatggat ggaaagatcc atacagcagc
      121 attaagtatt caagacgaca gaataattat gttagtgcaa caggcagcaa tgaagtgttt
      181 acgccacaag tgtttgttaa cggcaacaca ggctttgtag gctctgacag caaacgtctg
      241 aattcagaaa ttgaaaaagg tcttaaatca gttgctaaac aaaaactgac ggttacagtt
      301 ttacaaaccc taaatgacac tttacatctt aactatactg caagtaaagc cgatgccaat
      361 gctaatatta ttgtagccgt agtacaaaaa agtgttacaa caaaaattac aaaaggtgaa
      421 aacataggaa gaacacttgt tcaagacaac gtagtgagac tttacgaagt atatcctgtt
      481 gccacaaaag gaacactgct tttaaaaacc aataaacttg cactcaatga gaattttcaa
      541 ctcattgcat tcatacaaaa caaacaaagc aaaaaaattt ctgcagctac tgcgctaacg
      601 ctttagttac tttttcctga tcaggtaaaa gcattctctc ctgttatatc cattcctgta
      661 atgagcaaat gaatgtcatg cgtaccttca taagtaatta ccgactcaag gttcatcatg
      721 tggcgcatga taggatattc tcctgtgatg cccatgccgc catgtatctg tcttgcttcg
      781 cgggcacacc acaatgcagt gtgaacgttg ttacgtttag ccatacttat ttgtgcagga
      841 ttggctctgt tttcattctt taattttcca agccgccata ccaacaactg acctttggta
      901 atttctgtca gcatttcggc aagttttttc tgctgaagct gaaagcctcc aattggtctt
      961 ccgaattgtt tacgctcttt cgcatagcgt aaagcgctgt cataacaatc catggctgca
     1021 cctaaagctc cccaagcaat gccataacgt gcactgttca aacatcctaa tggtcctttt
     1081 aaacctttta cgttaggcaa taaattttct ttaggcactt ctacattatc aaaaaccaac
     1141 tcacctgttg cactagcacg taagctccat ttgccatgtg tttcaggagt tgtaaaacct
     1201 ttcatgccac gttcaactat caagcctctg atgacacctt cttcgtcttt tgcccaaact
     1261 actgcaacct gtgcaaacgg tgcattgctt atccacatct ttgcaccatt caataaataa
     1321 tgatcgcctt tatctttaat attggtaacc ataccaccgg gatttgaacc atggtcaggc
     1381 tctgttaaac caaaacatcc catccattct cctgatgcaa gtttgggtaa atatttttta
     1441 cgctgtgctt cgcttccgta ggtataaata ggatacatca ctaacgaact ctgtaccgat
     1501 gcggttgaac ggataccact gtcgccacgc tcaagttcct gcataattaa accatagctg
     1561 atctgatcca atccaccacc accatactct gccggaatat aagggccgaa tgcacctacc
     1621 tctgccaaac cttttattaa atgctttggg aactcacact tctgacaggc atcttcaata
     1681 atcggagaaa cctctttctt tacccactcg cgcacactgt cacgaatcag cttatgttcg
     1741 tccgttaata attcatccat taaaaaataa tccggtgcct gaaatctgtc ttttgacata
     1801 ctttaaattt tagtctgcaa aaataggatt tttggttgaa ggaaaatcag cttttgaagg
     1861 aattaaattt gtaagaataa tctcctcaaa ttaataacct aaaatacttc ccagccattg
     1921 ttcggcttct tcaacagtca ttgatttacg ttttgcataa tcctcaactt ggtcgcgctg
     1981 aatttttcct actgcaaaat aatgcgattc gggatgtgca aaatacaatc cactgacggc
     2041 agcagttgga aacatagcca aatgttctgt aagcgtaatg cctgtatttt tttctacatc
     2101 aagtagtttc caaagtgtgt atttctctgt atggtcgggc tgtgccggat agcctgcagc
     2161 aggacgaatg ccctgatact tttctttgat taaatcctga cgattcagat tttcatctgt
     2221 tgtataagcc caaaactctt tgcgtacccg ctcatgcagg tgttcggcaa atgcttcggc
     2281 taatcggtcg gccaacactt ttaacatgat ggatgaataa tcatcatgat cgtctgcaaa
     2341 tttctttaac catttttcta ttccaaaacc tgttgacaca gcaaaggctc cgatataatc
     2401 tgaaggacga ataaaatctg ccaatgcaat attcttctgt cctgttgctc tctctgactg
     2461 ctgacggata gtatgaagtg ttgcaatttt attttctctt ctttcatcaa acacttcaat
     2521 gtcatcaccc ttgttcgctg caggaaaaat tccgaataca gcatttgctg taagccattt
     2581 ttctttaata atacgttgca gcattgtttg tgcatcatca aaaagttttt gcgcttcctg
     2641 acctcgttga gggtcgctca atattttcgg atagctgcct ttcatttccc atgcaataaa
     2701 aaatggtgtc cagtcaatat attctgtaag caatgacaaa tcatagttgc tgaaaacttt
     2761 tactccttta aattttggtt cagtaacaac ggctccattc aattcatatt tattttgttg
     2821 cgcctctttc aaagataaaa attttcctct tgatcgctct gccttatgat aggagcgtgt
     2881 tctttcattc tccgctttta tatcttcaat aaatttttct ttttgttctg cggaaagtaa
     2941 attactcaca acaggcacac ttcgtgaagc atcattgaca tgcacaacaa cgcctgaata
     3001 gtttggctca atctttacgg cagtatgtac cttacttgtg gtagctccac caatcagcaa
     3061 tggcattgta aatttttctc gctccatttc tttggcaaca tgtaccattt catcaagaga
     3121 aggagtaatc aatccactaa caccaataat atctgcttga tgatcgcgtg cagcctgcag
     3181 aattttatct gctgcaacca ttacacccaa atcaacaatt tcgtaattgt tgcaggcaag
     3241 tacaacacca acaatatttt ttccaatatc atgtacatcg cctttaactg ttgccagaac
     3301 aatctttcct tttgactgac cttttacttt ttctgcttca ataaaaggtg tcagataagc
     3361 cactgccttt ttcatcacac gtgcacttct tactacctga ggcagaaaca tttttccttg
     3421 tccgaataag tcacctacca cacccatacc aatcatcaga tgaccttcaa taacctcaag
     3481 tgctttagat acctgctgcc gcgcctcttc catatcctga tctacaaatt cgtcaatacc
     3541 ttttatcaat gaatgtgtaa tacgtgattg caggtcttca ctgcgccagg cgttggccga
     3601 ttgatttcct ttctttacat ttttatcagt tgtggtctgc gaaagtttta actcttcggc
     3661 ttttgccaag agtttttcag ttgcatcttc tctcctgtct aagataacat cttcaaccaa
     3721 tgcaagtaaa tctttttcta tttcatcata aacgataagt tgcgatgggt ttactatgcc
     3781 catatccatt ccatttttta ttccataata aagaaacact gcatgaatgg cttcgcgaat
     3841 ggggtcatta ccacgaaatg aaaaagaaac attacttaca ccacctgaaa tttttgcaag
     3901 aggaagattt tctttaatcc atttacttgc ttcaaaataa cttattgcat tctgacgatg
     3961 ttcttcaagt cctgtggcaa caggaaaaat gttcgggtcg aaaattacat cctgagcagg
     4021 gaagttaact ttgttaacta aaatatcata agcacgtttt gctatttcaa ttctacgtgg
     4081 tgtactatcg gcttgtccgt tttcatcaaa gagcataacg ataactgcag caccatattt
     4141 ttttactgtc tttgcttcat agataaattg atcttcgcca ccttttaatg agatggagtt
     4201 tacaattcct tttccctgaa tacattttaa tccggcttcg agcacttcaa actttgaaga
     4261 gtcaatcatc accggcactt ttgaaatatc aggctctgca gcaatcaggt tcagaaatgt
     4321 tgtcatggct tcaacggcat caagcatacc ctcgtccatg ttaatgtcaa ttgcctgcgc
     4381 acccccttca acctgctcgc gtgctacact caatgcttca tcgtacttat tatcttttat
     4441 caggcgaaga aattttttag agcctgtaac attggtgcgt tcacctacat tcataaaatt
     4501 tgaatcaggc ctgagtgtta ctgcttctaa tccgctcaat cgcagataag gttctatggt
     4561 tggtatagct cttggcttat gattctttgc atgctctgca atgtgtttga tgtgttcggg
     4621 cgtggtgccg caacaaccac caataatatt tacaaatcca ctttctaaaa aatcatctat
     4681 ctgcacacac atctgatgcg gtgtttcatc gtactcacca aactgattgg gcagccccgc
     4741 attgggatag catgaaatat aaaatggtga tttcgcacta agctcttcca gataagggcg
     4801 catttctttt gcacctaatg cacaattgag gcctatgctt aatagattaa catgcgagac
     4861 agagttcaga aatgcctcca ccgtttgtcc gcttaatgta cgtccgctgg catctgtaat
     4921 tgttccggaa atcatgacag gcaaatcaac ctttttttgt tccattacat tctgaatggc
     4981 tactaatgct gctttggcat tgagtgtatc aaaaattgtt tcaactaaaa gtaaatctgc
     5041 accaccatca accaagccat ttatctgctc cgtataagca tctaccatct gatcccagct
     5101 aaccgctcgg aaacccggcc tgtttacatc gggcgagagt gacaaagttc tgtttgttgg
     5161 accaacagca ccagcaacat aaaacttttt atctgactga tgtttctttt tgaatgattc
     5221 aaccgccttc ttagcaattt tagccgactc aaaatttaac tcataagcca atgactccat
     5281 gtggtaatcg gctaatgaaa ttttctgcgc attaaaagtg ttagtttcaa taatatcagc
     5341 tcctgcttcg agatattcca agtgaatggc ttcaataatt tcgggcttgg tgagtgagag
     5401 caaatcgtta ttccccttta agtcactttt ccaatctgca aacctttcac ctcgataatc
     5461 ttcctcactt aatttatatc gctgaatcat agtgcccatg gcaccatcta taattaatat
     5521 tcttttttct aactcctgtc taatattttt catttcacta aagtgttgca acaattctaa
     5581 ttaatgtttt tctatttaca agctctgatc atttcacgtt tcccttttgg tccgggcaat
     5641 gcttcaacag caaacccaac tttttttaat gttcgcttta cttcaccatt agcacaataa
     5701 gttaccagca tagctcctct attgcatgac tcataaacct tatgaagcac ttcaaaagac
     5761 cataactccg gctggtgtgt tggtgaaaat gcatcgtaat agacaaggtc ataaccacca
     5821 tctaatttca actccagcat atcagcatta attttatgca aaacaaaatc gccaaaagtt
     5881 acattctcga accagttgca gtcatgcatt ttttgaaaca tgggtagcaa tgcttttcca
     5941 aagtattcaa aataatttaa cttgtttatt attgccattg gcaagggcga tgactcaata
     6001 gtggtataat gaattggctt ctgcaacaca taacattctc tgtatgtgag tatggcattg
     6061 agtcctgtgc caaatcccat ttcaagaatt ttaatgtcag gaaggctttt attcaaggca
     6121 agcaaaccat ttctaataaa cacatgcata ctttcctgca tggctccatg caatgaatga
     6181 tagtgttcat taatccgggt gttgattagc gtatcaatac cttcttttgt ttttacaatc
     6241 tcaatggagt ccatctcctt aatttaaatc agatttaaaa taaaaaaaat cttttccgaa
     6301 aagccgaaaa agattgaacg tttttaatct gatttcgact tatctgcttc ccttagggaa
     6361 tgtgaattgg caccttttct gccagaggca gatggttgtc aaggcatcgc agggcacttc
     6421 cctccgcctt tctcgataag cattttgaaa agaacaaggt gcaaatatac agcataccaa
     6481 tcatttgaac aaaacaattg cattgaattt ttacactgta cttgaatagt tgtactatat
     6541 taaac
//
LOCUS       DAORQT010000084         6495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1899_length_6495_cov_8.486335, whole genome shotgun sequence.
ACCESSION   DAORQT010000084 DAORQT010000000
VERSION     DAORQT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1899_length_6495_cov_8.486335"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4180
                     /locus_tag="PK134_04335"
     CDS             <1..4180
                     /locus_tag="PK134_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38107.1"
                     /translation="ITITASGGTGPLSYSIDNGISSVSSNHFTMLAAGNYNIILTDSV
                     GCITSSNIILNEPDSIIIQQAVTAATCNQNNGSFQIVVNGGTGILQLSLDSINFSPGT
                     IFNNLIAGIYQLHVTDANQCYATKLISVPNQAAPVLTNLSKGDVTCFGDSTGFISLSA
                     SGGTGLLNYSIDNGVTYQALPNFNNLPTGSYHIKISDQNLCSTDTIIQLTSPDDITAT
                     LTITNANCQQSNGSITVNAQGGNGTLLFSLNGGPFSQQTYFGSLPAGTQQLQVKDGMM
                     CDKNIPVQINTINGPTLDTVITQNLNCFGINNGTISILASGGTGALGYSLNNQSFLNT
                     PNFNQLSAGNYHVSVVDSAGCLVSGLVQLTTPSAIQVTPTVTNPACGQNNGSLNLQLA
                     GGTGTLLTSLNGSSYNNITNYSNLYAGSYTLKIKDANNCEVQRAVSLSNILAPKLIGS
                     VLKQVKCFNANDGEIKILASGGTGVLTYSIDNGITTQISNTFSNLTAGTYSASVIDQN
                     NCRSDTTITLLQPDSLQLLLNITPETCSAGNGSITVSSTGGTSPYLFSSDSGQTFYNV
                     AGFAQLDSNIYYIIVKDSKNCRTLNQVNITNLTGPQIVSLTTTNVSCYGLQDGAVQIA
                     TAGGNGTLSFSTNNFITQQTDTFFNALLPGNYTVYVSDTNNCKSNHFFSISQPTVVQS
                     NIVSTQPSCFGYNNGSLIVTANGGTPPYNILWSSGSTDFNLSGIVSGQYSFIITDAHN
                     CNKSDSIYLSSPTVLAANHTTQNGTCSGNANGSALVSVTGGTPPYQYNWSPIAGNTNY
                     ANALPAGIYTVDVTDKNNCTLTEQIQISTPAPIANTFAKNDVSCFGGSNGMITANALG
                     GTAPYSYLWSYQQQTNDTILNLPVGSYSVTITDANGCDNFFTEQISSPQQIKPNSIIN
                     NVSCNGFNDASVTLNVQGGVAPYSYQWNNGVTQSLNTNLAAGIYVVTVTDANNCNRVK
                     AFTITEPLPILLSIYPVDTICIGQQVLLNAIASGGTGTYNWLWNNQAAASTISVTPSV
                     TTNYFVSVTDSNNCPSKTDSILVIVFPPLGITATGYDTICAGQSAVLSSIANGGNGGP
                     YSYLWSNGSDSATVNVSPSVTTTYTVSITDNCTQMPATAMVPVVVNETPSLDVSPMTE
                     QGCMPLEVQFLNSSTYPQGTIFTWNFGDGQTTDSITPLHIYNADGIYTVSVQAITPDN
                     CVSNIIISNPIEVYPLPVASFNISPESISILHPEINISDSSLLGSIWFYDFGDGQTST
                     LQNPNHIYQDTGRFTVIQIVESEFGCRDTSKREAIVEGVFTVYIPNAFTPNNDGSNDY
                     FFANGYGITELTMQIFNRWGNKVFETPSVTGRWNGIDTSGKICPQGVYVYTAKIKDQF
                     GIYHTYNGQVTLIR"
     gene            complement(4173..5459)
                     /locus_tag="PK134_04340"
     CDS             complement(4173..5459)
                     /locus_tag="PK134_04340"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HRB38108.1"
                     /translation="MAEIVRMPKMSDTMTEGVVAKWHKKIGDQIKSGELVAEIETDKA
                     TMEFESYQNGTLLYIGVEEGKAAPVDSILAILGKPGEDFKPLLEAESKAKTATAAVPE
                     KTEAKPAAKVVAETPVKTVAAPKVEAKPVTQPVVASESDSRVKASPLARSLAGQKGLD
                     LTMIKGSGDNGRIVKRDVEWFKQGNVVAGSYTSAIRTQESFEETPVSQMRKTIARRLS
                     ESKFTAPHFYLTLDIEMSRCMDAREAINAATGSKVSFNDFVIKACAAALQQHPKVNSS
                     WLGDTIRTNHHIHIGVAVAVDEGLLVPVVRFADSKTLSQINAEVKTYAQKAKDKKLQP
                     ADWEGNTFTISNLGMFDIEEFTAIINPPDACILAVGAIKAVPVVKDGSVIAGNVMRVT
                     LSCDHRVVDGVTGAKFLQTVKGFLENPVLLLGQASI"
     gene            complement(5488..6486)
                     /gene="pdhA"
                     /locus_tag="PK134_04345"
     CDS             complement(5488..6486)
                     /gene="pdhA"
                     /locus_tag="PK134_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRB38109.1"
                     /translation="MAKQKFTKETYLFWYEQMLLMRRFEERSGQLYGMQKIKGFCHLY
                     IGQEAVVAGAMSVIKPTDNMITAYRDHAHALGKGTSARAVMAELFAKATGCSKGKGGS
                     MHLFDAANRFFGGHGIVGGQIPLGAGIAFAEKYKGTDSVTLCYMGDGAVRQGAFHEAL
                     NMAMTWKLPVVFIIENNNYAMGTSVERTSNVTDLYELGTAFNMPSEPVDGMMVETVHE
                     AMDKAVSHCRKGNGPYLLEMNTYRYKGHSMSDPAKYRSKEELEKYKAQDPIEEVLKVI
                     QKNKLATQEEIDAIDKKVHDIVEDSVTFAEESPFPDVDELYKDVYEQSDYPYIIED"
BASE COUNT         1976 a         1390 c         1111 g         2018 t
ORIGIN      
        1 aattacaatt actgcttcag gaggaaccgg tccactatca tatagtattg acaatggtat
       61 ttcatctgtt tcgagtaatc actttacaat gctagcagca ggaaattaca acatcattct
      121 tactgatagt gttggctgta ttacttcatc aaatattata ctaaacgaac ctgattcgat
      181 aataattcag caagcagtta cagcagctac ctgcaatcag aataatggtt cttttcaaat
      241 agtcgttaat ggtggcacag gaatacttca actttcatta gatagcatca actttagtcc
      301 aggcacaatt tttaacaacc ttatagcagg aatttatcag ctacatgtta ctgatgccaa
      361 ccaatgttat gcaaccaaat taatttctgt tccaaatcag gctgcacctg tacttactaa
      421 tcttagtaaa ggtgatgtta catgctttgg tgacagcact ggttttattt cattatcggc
      481 tagtggcgga acaggattgc tgaattatag tattgacaat ggtgtaacct atcaggcatt
      541 gccgaacttc aacaatcttc ctaccggttc ttatcatata aaaatctcag accagaatct
      601 ttgttcaacc gatacaataa ttcaattgac atcacctgat gatattacag cgacattaac
      661 tatcaccaat gctaactgcc aacaaagtaa tggcagtata accgttaatg cacaaggcgg
      721 aaacggaaca ttattattca gtcttaatgg cggcccattc agtcagcaga catattttgg
      781 ttcgctaccg gcagggacac aacagttgca ggtgaaggat ggcatgatgt gcgataaaaa
      841 tattcctgta cagataaaca ctatcaatgg ccctacatta gatactgtaa taacacaaaa
      901 tctaaattgc tttggaatta ataatggcac aatttcaatt ctggcatcag gaggtaccgg
      961 tgcattaggt tatagtttaa acaatcaatc atttttaaac actcctaatt tcaatcagct
     1021 atctgcaggc aactatcatg tatcagtagt tgatagtgca ggatgtttag tatcgggact
     1081 tgtacaatta acaacaccgt ctgcaattca ggtcacacca actgttacta atcccgcatg
     1141 cggacaaaac aatggttcac ttaatttaca attagccgga ggcaccggaa ctttacttac
     1201 ttcattgaat ggatcttcat ataacaacat tacaaattac tcgaatcttt atgcaggaag
     1261 ttataccctt aaaattaaag atgctaataa ttgcgaagtg cagcgtgcag tttcactaag
     1321 caacattctt gcaccaaagc ttattggatc tgtattaaag caggttaaat gttttaatgc
     1381 caatgatggt gaaataaaaa ttctggcaag tggaggaaca ggtgttttaa cttacagtat
     1441 tgacaatggg ataaccacac aaatatcaaa cacatttagc aacttaactg caggcacgta
     1501 ctccgcctct gtaatagatc agaacaattg cagaagcgat acaaccatta ctttattgca
     1561 gcctgactct ttacaactgt tattaaacat tactcctgaa acatgttcag ccggtaatgg
     1621 aagtattact gtttcatcaa caggaggcac ttctccatat ttattttcat cagacagcgg
     1681 acaaacattt tataatgtag caggatttgc acagctcgac agcaatattt attacataat
     1741 agttaaagat tcaaagaact gtcgcacttt aaatcaggtt aatattacaa acctaaccgg
     1801 gcctcaaatc gtttctttaa caacaacgaa tgtttcatgc tatggtttgc aggatggtgc
     1861 agtgcaaatt gcaactgcag gtggaaatgg cactttaagt ttttcaacaa ataattttat
     1921 tacacaacag acagacactt tctttaatgc tttacttccg ggtaactata ctgtttatgt
     1981 aagtgatact aacaattgca aaagcaatca tttcttttct ataagtcagc ctacggttgt
     2041 acaaagtaac attgtctcaa cacaaccttc ctgtttcgga tacaataatg gctcactcat
     2101 tgtaacagca aatggtggaa caccacctta taatattttg tggtcgtcag gaagcactga
     2161 cttcaatctt tcaggtattg tatccgggca atactcgttt attataacag atgcacataa
     2221 ctgcaacaaa tcggacagca tatatttatc aagtccaaca gttttagctg caaatcacac
     2281 tacacaaaac ggaacatgtt caggcaatgc aaatggctct gcattagtgt ctgtaacagg
     2341 tggcacacca ccctatcaat acaactggtc gccaattgca ggaaacacca actacgccaa
     2401 tgccctaccc gctggcatat acactgttga tgttactgat aaaaataatt gtactctgac
     2461 tgaacaaatt caaatttcta cacctgcacc aattgcaaat acttttgcaa aaaatgatgt
     2521 ttcatgcttt ggaggaagca atggaatgat aacagcaaat gccttagggg gaactgcacc
     2581 ttacagctat ttgtggagct atcagcaaca aacaaatgat accatcctaa acctgcctgt
     2641 aggcagctat tcagtgacca tcacagatgc caacggttgc gataattttt ttacagaaca
     2701 aatttcttca ccacaacaga ttaaacctaa ttcaataatt aacaatgttt catgtaatgg
     2761 attcaatgat gcatctgtta cactaaacgt tcagggaggt gttgctcctt atagttatca
     2821 atggaacaat ggagttacac aatcattaaa tacgaatctt gcagcaggaa tttatgtagt
     2881 tactgttaca gatgcaaata actgcaacag agttaaagcg tttacaataa ctgaaccact
     2941 gccaatttta ctttcaattt atcctgtgga cactatctgt attggtcagc aggtattgtt
     3001 aaatgcaata gcatctggtg ggacaggaac ctacaattgg ctttggaata accaagccgc
     3061 tgcatcaaca atttcagtca ctccatcagt aacaacaaac tattttgttt ctgttacaga
     3121 cagcaacaac tgcccatcta aaactgatag tattttagta attgtatttc ctccgttagg
     3181 aattactgca acagggtatg atacaatttg cgcaggacag tctgctgttc tgtcatcaat
     3241 tgctaatggt ggaaatggtg gaccgtatag ttatctctgg tcaaatggaa gtgatagcgc
     3301 aactgtaaat gtgtcaccat cggttacaac aacatatact gtaagcatta cagacaactg
     3361 tacgcaaatg ccggcaacag caatggtacc ggttgtggta aacgaaacac cctcacttga
     3421 tgtttctccg atgactgagc agggttgtat gccattggaa gtgcaattcc tcaatagctc
     3481 cacctatcca caggggacaa tatttacctg gaattttggc gatggtcaaa caactgactc
     3541 cattacacca ttacatattt acaacgctga cggcatttac acagtaagcg ttcaggcaat
     3601 aactcctgat aattgtgtaa gcaatattat catttcgaat ccaattgaag tttatccgtt
     3661 gccggttgcc tcattcaaca taagccctga aagtatttct attttacatc ctgaaataaa
     3721 tatttctgac agtagtttgc ttggctccat ctggttttat gattttggag acggacaaac
     3781 ttcaactttg caaaatccta atcacatcta tcaggatacc ggaaggttta ccgtcattca
     3841 aattgtagaa agtgagtttg gctgtcgcga cacttcaaaa agagaggcta ttgttgaagg
     3901 tgtttttact gtttatatcc caaatgcatt tacgccaaat aatgatggca gtaacgatta
     3961 tttctttgcc aacggttacg gcattaccga actgacaatg cagatattta accgttgggg
     4021 aaataaagtt tttgaaacgc catcagtaac cggccgatgg aatggcattg acacttcagg
     4081 taaaatttgt ccgcaaggtg tttatgttta tacggccaaa atcaaagatc agtttggcat
     4141 atatcacacc tacaatggtc aggttacgtt aatcagatag acgcttgccc taaaagcaat
     4201 acaggatttt ctaagaatcc ttttacagtc tgcaaaaact tagctcctgt aacaccatca
     4261 actacacgat gatcgcagct aagcgtaaca cgcatcacat ttcctgcaat aacactacca
     4321 tctttcacaa ctggaacagc tttaattgca cctactgcaa gtatgcaggc atctggtgga
     4381 ttgataattg ctgtgaactc ttcaatatca aacatgccca gattagaaat tgtaaaagta
     4441 tttccttccc agtctgcagg ttgtagtttt ttatcctttg ctttctgcgc ataagttttc
     4501 acttcggcat taatctgtga aagtgttttt gaatctgcaa aacgtacaac aggcacaagt
     4561 aagccttcat caactgccac tgcaacacca atatggatgt gatgattggt tctgatagta
     4621 tcgcccaacc aagatgagtt tactttagga tgctgttgca atgcagcggc acatgcttta
     4681 atcacaaaat cattgaatga aactttgctt cctgtggctg cattaatagc ctcgcgcgca
     4741 tccatacatc ttgacatttc gatatctaat gtaagataaa aatgtggtgc agtaaattta
     4801 ctttcgctta atcttcgtgc aatggtttta cgcatttgcg aaacaggtgt ttcttcaaaa
     4861 gactcctgtg tgcgtattgc cgaagtataa cttcctgcaa caacattgcc ttgtttaaac
     4921 cattccacat cacgtttcac aatcctgcca ttatctccac taccttttat cattgttaga
     4981 tcaagacctt tctgccctgc aagtgatcgt gctaatggtg atgcttttac tctactatca
     5041 gattctgatg ctactactgg ttgagtaaca ggttttgctt ctacttttgg tgccgcaaca
     5101 gtttttaccg gagtttcagc aacaaccttc gctgcaggtt ttgcctcagt cttttccggc
     5161 acagcagctg ttgccgtttt tgcttttgac tcagcttcaa gcaatggctt aaaatcttca
     5221 cccggcttac ctaatattgc taatatacta tcaacaggcg cagcttttcc ttcttcaaca
     5281 ccaatataaa gtaatgttcc gttttgataa ctttcaaact ccatagtggc tttatcggtt
     5341 tcaatctctg caaccaattc gccagacttt atctggtcac caattttttt atgccattta
     5401 gcaacaacgc cttctgtcat ggtgtcgctc attttgggca ttctaactat ttctgccatg
     5461 tttgatttta cttgtaatgc ttgttaatta atcttcaata atgtaaggat aatcggattg
     5521 ctcatagaca tctttataca gttcatctac atcaggaaat ggtgattctt ctgcaaaggt
     5581 cacactgtct tcaacaatat cgtgaacttt tttgtcaatc gcatctatct cttcctgtgt
     5641 tgcaagttta tttttctgta ttactttcag tacttcttct atgggatctt gtgctttata
     5701 tttttccagc tcttccttac ttctgtattt tgcaggatca ctcatagagt gtcctttata
     5761 acgatacgta ttcatctcta acaaatacgg accatttcct tttctgcaat gacttactgc
     5821 tttatccata gcctcatgca cagtttctac catcatacca tcaacaggtt cgctgggcat
     5881 attaaatgca gtacctaatt catataaatc ggtaacatta ctggttcgtt ctacagaagt
     5941 acccatagca taattattat tctcgataat aaaaactacc ggtagtttcc atgtcattgc
     6001 catattcaat gcttcatgaa atgcaccttg acgcactgca ccatcaccca tataacataa
     6061 agtcacacta tctgtacctt tatatttttc ggcaaatgca atgccagcac ctaatggaat
     6121 ttgtccgcca acaataccgt gtccgccaaa aaaacgattt gcagcatcaa acaagtgcat
     6181 acttccaccc ttacctttac tgcatccggt agctttagca aacagctctg ccatgactgc
     6241 tcttgccgaa gttccttttc ctaaagcatg tgcatgatca cgatatgctg taatcatatt
     6301 gtcggtaggt ttgataactg acattgcccc cgcaacaaca gcttcctgtc caatatacaa
     6361 atgacaaaat ccttttatct tttgcatacc atataactgt cctgaacgtt cttcgaacct
     6421 gcgcatgagc aacatttgtt cataccaaaa aagataggtt tcctttgtaa atttctgttt
     6481 agccatattc tttat
//
LOCUS       DAORQT010000085         6364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_1978_length_6364_cov_22.999841, whole genome shotgun sequence.
ACCESSION   DAORQT010000085 DAORQT010000000
VERSION     DAORQT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_6364_cov_22.999841"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..432)
                     /locus_tag="PK134_04350"
     CDS             complement(223..432)
                     /locus_tag="PK134_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRB38110.1"
                     /translation="MSKYEIIIYWSKDDKSYIAEVPELAGCMADGKSYQQALSNVEVI
                     INEWLETAKQLNRTIPKPKGKLMYA"
     gene            complement(429..680)
                     /locus_tag="PK134_04355"
     CDS             complement(429..680)
                     /locus_tag="PK134_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015191801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRB38111.1"
                     /translation="MSKYEKLLFKLLSGKNDTNFAFSDLCKLLDELGFNKRIKGSHHI
                     YYKNNVDEIINIQPDKAKAKPYQVRQVRNLIIKYKLIDI"
     gene            complement(875..2317)
                     /locus_tag="PK134_04360"
     CDS             complement(875..2317)
                     /locus_tag="PK134_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRB38112.1"
                     /translation="MKLPFKKLTTLVLGSFIGPFIMTFFIALFVLLMQFLWKYIDDLV
                     GKGLDWWTITKLLGYTSTTLVPLALPLAILLSSIMTFGNLAEHYEIVATKSAGISLQR
                     LMRPLVVVAIIISLGAFYFSNNILPYSNLKMGSLLYDVRQQKPALFIKEGVFYNGIDG
                     YSIKIGKKADDGKSIKNIMIYDHTHNMGNKKVVIADSGTMVMTNDKRYLILTLINGHS
                     YEEQQNRKKNYDTNPLLRTQFQSETIRFDLSSFQLNRTNEDLFKDNYQMMNVKQLNAM
                     IDTFQLQAAQRKTRFARDIKPYYQFLKDSTLFIHVSNAIAKPFLTDSIKLNNTKFETA
                     LSQCRVIKSYIESADDDLESRNRAYVKYQIEWHRKFTLSIACLLLFLIGAPLGAIIRK
                     GGLGLPIVISIAFFLCYHIISITGEKMAKEGIISVERGMWQSSLVLLPIGIFMVYKAT
                     HDSALFDMDSYKSFFKKLFRKKRLDDFLPK"
     gene            complement(2337..2810)
                     /locus_tag="PK134_04365"
     CDS             complement(2337..2810)
                     /locus_tag="PK134_04365"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRB38113.1"
                     /translation="MSNHNHFMEEAVALSKKAITDNEGGPFGCVVVKDGKIIAKGNNK
                     VTSTNDPTAHAEVVAIREACKALNSFQLDGCEIYTSCEPCPMCLGAIYWARPDVVYFA
                     NTRQEAAAIGFDDEFIYDEINVHPTERKIKMVHLPNLQAAGVFNSWQNKSDKTEY"
     gene            complement(2814..3218)
                     /locus_tag="PK134_04370"
     CDS             complement(2814..3218)
                     /locus_tag="PK134_04370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRB38114.1"
                     /translation="MPTSEVTYIGELRVKSVHVFSGNEIITDAPLDNQGKAQAFSPTD
                     LLATSLANCMLTIMGIAARTHQFSIDGTRAEVTKTMAADPRRVSAIEVKLFFPKNNFS
                     DKEKKMIENAAHTCPVAYSLHPDIKQDISFNF"
     gene            complement(3221..4879)
                     /gene="recN"
                     /locus_tag="PK134_04375"
     CDS             complement(3221..4879)
                     /gene="recN"
                     /locus_tag="PK134_04375"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRB38115.1"
                     /translation="MLTRLAIKNYVLIRELDINFSDGLSIITGETGAGKSILLGAMGL
                     VLGERADAKTLFDKKGKCIIEAEFSISHYNLNDFFSQHDIDFESNTIIRREINSEGKS
                     RAFVNDTPVNLSVLKELSSYLVDVHSQHQTLTLNTSAFQLNVLDITAGNQKQLNDYSN
                     QFNEYKKLLKQLVELKTLESKSIAESEFLQFQFDELESAGLKQGEFEKIESELQLLNN
                     TENIVLTLDSTSDILSDGETNVLSNITQAVTRLNGIARLHPSLEDILKRIQSCNIELK
                     DISSELTALKSQFSLNPSQLEILNDRLSVYNKLQQKHRVKNADDLIVLQQEIANRLQT
                     FQSLTDDIEKCDKQTQDLYNKLKQIAAELHSSRQKAAQSFKKDILKLLKEVSLNDAQF
                     EVDLTATEELTATGTTAVRFLFSANKGVTVMELAKVASGGELSRLMLCIKALIANKTS
                     MPTIIFDEIDTGISGEVAHKVGNLIEQLSESRQVLAITHLPQMAGKGKTHYFVFKHIE
                     KGIAQSRIKTLSAEERVVEIAKMLSGEEVTDASLQNARHLLIKS"
     gene            complement(4913..6130)
                     /locus_tag="PK134_04380"
     CDS             complement(4913..6130)
                     /locus_tag="PK134_04380"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRB38116.1"
                     /translation="MTPDNTMKLNSIEEAIADLKNGKAVIVVDDADRENEGDFITAAH
                     NATPEMINFMASHGRGLICATLTEQRCEELALSMMVATNTSSHETPFTVSVDLLGHGC
                     TTGISASDRSKTIKALIDPATKPSDLGRPGHIFPLKSKNGGVLRRAGHTEAGVDLARL
                     AGFEPAGVLVEIMNEDGTMARLPDLVKIAKKFDLKIISIEDLISYRLKTESLIDREVD
                     VKLPTAYGSFELFAYKQNTTGQEHLALVKGEWKKDEPILVRVHSSCLTGDIFGSCRCD
                     CGPQLHKAMQMIEKEGKGVIVYMNQEGRGIGLLNKLKAYKLQEAGKDTVEANQELGFK
                     PDERDYGVGAQILRDLGVSKLKLMTNNPTKRAGLIGYGLEIVEIIPLEIESNEHNRHY
                     LQTKRDKMGHTIK"
BASE COUNT         1811 a         1219 c         1183 g         2151 t
ORIGIN      
        1 gtaatttgaa ctccaatttc ttattcctta acttaatgac attgggcgca agtctgaaga
       61 cttgcaccag aggggaatat ctgtaggtgc gaggctgtgc ctcgttgcct attattttaa
      121 tggcttgcag ccataaaatt aatctctctt tcgctaaaag cgaaaataat aaaatgacac
      181 gagcgacacg ctcgcgctta caaagatggg cggcaggaaa ggtcaggcat acatcagttt
      241 tcctttgggt tttggaatgg tgcggtttaa ctgcttggct gtttccaacc actcgttaat
      301 aatcacttcc acatttgaca atgcctgttg atatgatttt ccgtcagcca tgcatcctgc
      361 cagttcaggt acttcggcaa tgtagctttt atcatcctta ctccaataaa taatgatttc
      421 atatttactc atatatcaat cagtttatat tttataatca gatttcttac ttgtctaacc
      481 tgataaggtt ttgctttcgc tttatcaggt tgaatattta ttatttcatc aacattattt
      541 ttataataaa tatgatggct gcctttaatt cttttattaa atcctaattc atccaacagt
      601 ttacataaat cgctaaaagc aaaattagtg tcattttttc cactcaacaa tttaaataac
      661 aacttttcat atttactcat aggcaatcaa gacaaaatag aaagagcaaa tatacaatta
      721 ttgatttcac tagacaaatc agcgtagcct ctgttctcag taggtgcgag gctgtgcctc
      781 gttacctatt attttaatgg cttgcagcca taaaattaat ctctctttcg ctaaaagcga
      841 aaatagttaa atgacacgag cgacacgctc gcacctactt aggtaaaaag tcatcaagcc
      901 gttttttccg gaacagtttt ttgaaaaatg acttataaga gtccatatca aacaaggctg
      961 agtcatgtgt ggctttgtaa accataaaaa ttccgatagg cagcaacacc aatgacgatt
     1021 gccacatgcc tctttctaca ctgatgattc cttcttttgc catcttttct cctgtgatgg
     1081 aaataatgtg atagcagagg aagaatgcaa tggaaattac aataggtagt ccaagaccgc
     1141 ctttgcggat gatggcacct aaaggtgctc caattaagaa cagcaataaa caggcaattg
     1201 ataaagtgaa tttgcgatgc cactcaattt gatattttac ataggcacga tttctgctct
     1261 ctaaatcatc atcagcactt tctatatacg atttgatgac acgacattgc gacaaagctg
     1321 tttcaaattt ggtgttgttt agtttgatgc tatcagttag aaaaggtttt gcaatggcat
     1381 tgctaacatg gataaacaat gttgagtcct ttaaaaactg ataatatggt tttatgtctc
     1441 tggcaaaacg tgtttttcgc tgtgctgcct gtaattgaaa agtatcaatc atggcattaa
     1501 gctgtttgac attcatcatc tgatagttgt ctttaaacag gtcttcattg gttctgttta
     1561 gctgaaaaga cgataagtca aaacgaatag tttcggattg gaattgcgtt ctgagtagtg
     1621 gattggtgtc gtaatttttt ttacggtttt gttgttcttc gtatgaatgc ccatttatta
     1681 aagttagaat aagataacgc ttatcatttg tcatcaccat agtgccacta tcggcaatca
     1741 caactttttt attacccatg ttgtgtgtat gatcataaat catgatgttt ttgattgatt
     1801 taccatcatc agctttttta cctattttaa tactgtagcc atcaatgcca ttgtaaaaaa
     1861 caccttcttt aataaacaat gccggctttt gttgacgcac atcataaagc agcgagccca
     1921 ttttaagatt actgtaaggt aaaatgttgt ttgaaaaata aaaggcgcct aaagaaatta
     1981 ttattgcaac cactactaat gggcgcatta acctttgtaa agaaatacct gccgattttg
     2041 tggcaacaat ctcatagtgt tccgcaaggt tgccaaaagt cattattgat gacaacaata
     2101 tagctaatgg caatgccaaa ggaactaagg tggttgatgt gtagccaaga agtttagtga
     2161 tggtccacca gtcgagtcct ttgccaacta aatcatcaat atacttccac aagaattgca
     2221 tcagcagcac aaagagtgct ataaaaaatg tcatgataaa aggaccgata aaagaaccta
     2281 atacaagtgt ggtgagtttc ttaaatggta atttcatcaa ttatctgttg ctgatatcaa
     2341 tactcggttt tgtcagattt attttgccat gaattaaaaa caccggcagc ctgcaggttg
     2401 ggtaaatgca ccatttttat ttttctctcg gtggggtgaa catttatttc atcataaata
     2461 aattcatcat caaaacctat tgcagcagct tcctgtctgg tattggcgaa gtaaacaaca
     2521 tccggtcgtg cccaataaat agctccaagg cacatagggc aaggctcgca ggaagtataa
     2581 atttcgcagc catctaactg aaaactgttg agtgctttac aagcctcgcg gatggcaact
     2641 acttctgcat gtgcggtagg gtcgttggta gaggtaactt tattatttcc tttagcaata
     2701 atttttccgt ctttaacaac aacacagcca aaagggccgc cttcattgtc agtgattgct
     2761 ttcttggaaa gggcaacagc ctcttccata aaatgattgt gattgctcat ttattaaaaa
     2821 ttaaaactga tatcttgttt gatatcggga tggagactgt atgcaacagg acaggtgtgg
     2881 gctgcatttt ctatcatttt cttttcctta tcagaaaaat tatttttcgg aaaaaacaac
     2941 ttcacttcaa tagcgctcac tcttctaggg tcagcggcca tagtttttgt tacttctgct
     3001 cgtgtgccat caattgaaaa ctgatgtgtt cttgcagcga tgcccataat agtgagcatg
     3061 cagttggcta gtgatgttgc taaaaggtcg gtgggggaga aggcctgtgc ttttccctga
     3121 ttgtcgagag gcgcatcggt gatgatttca tttcctgaaa aaacatgaac cgattttact
     3181 cttaattcgc ctatataggt aacttctgat gtgggcataa ttatgatttt ataagtagat
     3241 gtcgtgcatt ttggaggctt gcatctgtaa cctcttcacc gcttaacatt tttgcaattt
     3301 caacaactct ttcttcagca gaaagggttt ttattcttga ttgtgcaatt cctttttcaa
     3361 tatgtttaaa aacaaagtaa tgcgtttttc ctttgcccgc catttgcggt aggtgtgtta
     3421 tggcaagtac ttgtctgctt tctgaaagtt gttcaataag gttaccaact ttatgggcta
     3481 cctcaccgga gatgcctgtg tcaatctcat caaagatgat ggttggcatt gatgttttgt
     3541 tggctataag tgctttaata cataacatca gtcgtgaaag ttcaccaccg gaagcaacct
     3601 tcgccaattc cattaccgtt actcctttgt ttgcagaaaa taaaaagcgc actgcagttg
     3661 tgcccgttgc tgttaactct tctgtggctg tcaggtcaac ttcaaactga gcatcgttta
     3721 acgaaacttc tttcagtaat ttcaaaatat cttttttgaa actttgtgca gctttctgtc
     3781 tgctgctatg gagttctgcc gcaatttgtt ttagtttgtt atataaatcc tgagtttgtt
     3841 tatcgcattt ctcaatatcg tcagtaagcg attgaaaagt ttgaagtctg ttggcaattt
     3901 cctgctgcaa gactatcagg tcatcagcat tttttactct gtgtttttgt tgtagcttgt
     3961 tataaacaga aagtctgtca ttgagtattt caagctgtga tgggtttaaa ctaaactggc
     4021 ttttaagtgc agttaattcc gatgaaatat ctttcagttc aatgttgcac gactgaattc
     4081 ttttcagaat gtcttcaagc gatggatgta atcgtgcaat tccatttaaa cgggttacag
     4141 cttgtgtaat gtttgaaagc acatttgtct cgccatcaga caaaatatca cttgtgctgt
     4201 caagtgttaa tacaatattt tcagtattgt taagtagttg cagttctgat tcaatttttt
     4261 caaactcacc ttgctttaat cctgccgatt caagttcgtc aaactgaaac tgtaaaaact
     4321 cagattctgc aatactctta ctctccaatg ttttcagttc tacaagttgt ttcaataatt
     4381 ttttatattc attgaattgg ttgctgtaat catttaactg cttctgattg cctgctgtaa
     4441 tgtctaatac atttaactga aatgccgaag tatttaatgt caatgtctga tgttgcgaat
     4501 gaacatcaac caagtagcta ctgagttctt tcagaacaga aaggtttacg ggagtgtcgt
     4561 taacaaatgc acgcgatttt ccttctgaat taatttctct tctgatgatg gtgttggatt
     4621 caaaatctat atcatgctgc gaaaaaaaat cattcaggtt gtaatgagaa attgaaaatt
     4681 ctgcttcaat aatacattta ccttttttat cgaaaagtgt ttttgcatca gcacgttctc
     4741 ctaaaacaag ccccattgca cccagcagaa tagattttcc tgcacctgtc tcgcctgtga
     4801 taatacttaa cccatcagaa aaattaatat ccaattctct gataagcaca taatttttta
     4861 tggcaagacg ggttaacata caatgttgtt tcttaaaata atatcctgtt gattatttaa
     4921 ttgtgtgtcc cattttatcg cgctttgttt gcagataatg tcggttgtgt tcgttggact
     4981 caatttctaa aggaataatt tcaacaatct ctaagccata gccaatcaat ccggcacgct
     5041 ttgtagggtt gttggtcatg agttttaatt tagaaacacc taaatcgcgt agaatttgtg
     5101 cgccaacacc ataatctctt tcatcaggtt taaaacccag ttcctgattg gcttcaacag
     5161 tatcttttcc tgcttcctgc agtttatatg ctttaagttt attcagcaat ccgatgccac
     5221 gtccttcctg attcatataa acaattacac cctttccttc tttttctatc atctgcatgg
     5281 ctttatgtag ttgcggcccg cagtcgcagc ggcaagaacc aaaaatatca cctgtaaggc
     5341 aggaggagtg aaccctcact aaaataggct cgtctttttt ccattcgcct tttactaaag
     5401 caagatgctc ttgtcctgtt gtgttttgtt tataggcgaa aagctcaaag ctaccatagg
     5461 cagtaggcag tttaacatcc acttcccgat caatgagcga ttctgttttc agccggtagg
     5521 agataaggtc ttcaatagaa attattttca ggtcaaactt ttttgcaatt tttaccaaat
     5581 ctggaagtcg tgccattgtt ccatcttcat tcataatttc tacaagcaca cctgcaggct
     5641 cgaatccggc aagacgggca aggtcaacac ctgcttctgt atgtcctgct cttcttaaaa
     5701 cgccaccatt tttactttta agagggaaaa tatgtccggg gcggccaagg tctgaaggtt
     5761 ttgtggccgg gtcaattaat gctttaatgg ttttagagcg gtcggaggcc gaaattcctg
     5821 tggtgcatcc atgtccaagc aaatcaacag aaacggtaaa tggtgtttcg tgagaggaag
     5881 tatttgttgc caccatcatg ctcaaagcca actcttcgca gcgttgttct gtaagagtag
     5941 cacatataag gcctctgcca tggctggcca taaaatttat catttcagga gtggcattgt
     6001 gggcggcagt aataaagtcg ccctcatttt cgcggtcggc atcatcaact actatcaccg
     6061 ccttgccatt tttaagatcg gctatagctt cttctatact attcaatttc attgtattgt
     6121 caggagtcat tgtctctaaa tctgtttttg caaatttaca acaagctttt caatgtttag
     6181 ataaaattct tacagtagaa taggctggtt ttttgtcatt tttctgtatg ttaagatgaa
     6241 gtgaagattg taataggtat ataatcttac atacgtaatg tacgtttata gtagtgtaac
     6301 aaaaggaata aagcgaagat acatctatta caccactgct gctattattt tgctttttca
     6361 ataa
//
LOCUS       DAORQT010000086         6321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2003_length_6321_cov_8.951005, whole genome shotgun sequence.
ACCESSION   DAORQT010000086 DAORQT010000000
VERSION     DAORQT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2003_length_6321_cov_8.951005"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>255
                     /locus_tag="PK134_04385"
     CDS             <1..>255
                     /locus_tag="PK134_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38117.1"
                     /translation="IKADPNFIEAYMLLGDLQADNNNIPAAIEAYKKAVAINPRFFPN
                     NFYALGRYELESGRYDDALVHFNEFVKMETAKPDKKEEAKN"
     assembly_gap    256..313
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <314..1734
                     /locus_tag="PK134_04390"
     CDS             <314..1734
                     /locus_tag="PK134_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB38118.1"
                     /translation="INSDMDEYFPSITVDSKRMLFTRKIKSDIYEWQEDFYISTLQQD
                     SSWGKAKNVGAPVNTELNEGAPSYSADGQLIFFTACDRPESKGSCDIYYSRKFGDVWG
                     KPINLGSPVNTGAWESQPSFSSDGKTLYFVRGSNSRTNDQDIFMSEVSEKGWSTPVKL
                     NNNINTAGKEEAVFIHPDNQTLYFVSDGWPGMGGSDIFMSKRQADGQWGKAVNLGYPI
                     NTFANEAGLIVSPSGDKAYFSSNREGGLGGLDIYSFDLYDKIKPDPVSYAIGYVYDDE
                     TKKPADAKFEIIDIESGKTVVESYSDKNTGEFMVTLPYGKNYMLNVSKRKYLFYSDHF
                     EMDKPSDIKNAKQLIVRLKPVKDGSTMALNNIFFDTNSAELKPESKTELNKLVSFIKS
                     NAIAKLEIGGHTDNVGDDKANMLLSDKRAKSVCDYLITSGIEASRLISRGYGETKPVS
                     DNTTEEGKAKNRRTEITILSVH"
     gene            1832..3400
                     /gene="dnaB"
                     /locus_tag="PK134_04395"
     CDS             1832..3400
                     /gene="dnaB"
                     /locus_tag="PK134_04395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRB38119.1"
                     /translation="MEENVEAGRSAKKKVAPKSLTNQQIALGLGKIPPQAVDMEEAVL
                     GALMLEKNALTAVIDILHTEAFYKEAHQKIFKVIHDLFHKAEPIDILTVTHALKKSGD
                     LELVGGAFYITQLTNRIASAANIEYHARIILQKFIQRELIRISSETIKSAYEDTSDVL
                     KMLDDAEKNLFSIAEGNIRRNYENMNSLITKAIREIEIASKQTQGITGVESGFTDLDR
                     ITAGWQKSDLIIVAARPGMGKTAFTLSLAANAALQFNRPVAFFSLEMSSVQLVNRLLS
                     SQAEITSEAIRKGQLAEHQWQQLNSKIAPLSDAPIFIDDTPALSVFELRAKCRRLVAE
                     KKVQLIIIDYMQLMVAGTDNRNGNREQEISTISRSLKAIAKELEVPIICLSQLSREVE
                     KRGTASKRPQLSDLRESGAIEQDADMVMFIYRPEYYGMDVDDEGHSTKNTAEIIIAKN
                     RNGSLNTVKLTFIGQYTKFKDRELENQTPQDKAIYGYTDESNKVRIPNPNVEAENRYT
                     RGSKMNDMPDEDPF"
     gene            complement(3407..4309)
                     /gene="rfbD"
                     /locus_tag="PK134_04400"
     CDS             complement(3407..4309)
                     /gene="rfbD"
                     /locus_tag="PK134_04400"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRB38120.1"
                     /translation="MLRILLTGSNGLLGQKIVYAAIKHSDIQLLATSKGENRLHTKAG
                     YDYVSMDITNADEVTAIVENFKPHCIINTAAMTNVDACEKDQENCWKMNVEAVRTMTE
                     LCQLHDIHFIHLSTDFVFDGEKGNYSEEDAPNPLSHYARSKFESEKVVQQAEIDSAIL
                     RTIIIYGVVDDKQRSNVVIWTINSLRQQKNINVISDQMRSPTLAEDLADACLQAALKR
                     ATGIYHVSGSETMSILEMVNRTADFFQLDKKYIHPITTAQLNQPAKRPLLTGFNIDKA
                     KRELNFQPHTLQQGLEIVKSQLEN"
     gene            complement(4322..>6321)
                     /locus_tag="PK134_04405"
     CDS             complement(4322..>6321)
                     /locus_tag="PK134_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38121.1"
                     /translation="KKNVELKQVEVSAERQTKKTEVKIAVESVTPQEIKSLPSLGAEG
                     DLVQYMQVVPGVVSSGDQGGQLYIRGGTPVQNKVLLDGMVIYNPFHSIGLFSVFDTDI
                     LKNTDVYTAGFGAQYGGRISSVMDITTRDGNKKRIGGKVSAGTFLSKVLLEGPIKKAK
                     DENDGTASFILSVKNSYLDQTSKSIYKYASDDGLPYSFNDIYGKVVVNSASGSKVSFF
                     GFNFQDKVNYSKVAKYDWSSSGGGMNFVLVPGASKVLLRGNLAFSSYKIKLTEAEEKP
                     RSSLVNGFNMGFNWLYFLNNDEINYGIEVQGFKTDFEYFNYAKRQINQTENTTEIGIF
                     FRYKKIINKLIIDPSVRLQYYASISEFSPEPRLGIKYNLSDNIRLKAAGGLYAQNLIA
                     ANSDRDVVNFFYGFLSAPDDLPSEFDGKSVKKKLQLARHAVVGVEYDISKHFDINIEG
                     YIKDFNQLTNINRDKVYDDTANNFDKPDSVKKSFIIEKGFTQGVDVVLKYEYRRMYAW
                     LVYSLGFSTRNTGNYEYAPHFDRRHNINIIGSYKLGKKLDWEVDARWNFGSGFPVTLN
                     QGFYPYLNFQDGLATDYTQNNGEMGVLYGPLNESRLPTYHRLDISIKKIFYLARHSEL
                     EVVASVVNVYNRKNVFYVDRIRHNVVNQLPIMPSLGASLTF"
BASE COUNT         2009 a         1149 c         1256 g         1849 t
ORIGIN      
        1 attaaagctg atccgaattt tattgaagca tacatgcttc ttggcgattt gcaagccgac
       61 aacaataaca tacctgccgc tattgaagct tataaaaaag ctgttgcaat aaaccctcgt
      121 tttttcccaa acaattttta tgcattagga aggtatgaat tggaatcagg aagatatgat
      181 gatgcattgg ttcacttcaa tgaatttgtg aaaatggaaa ctgcaaaacc tgacaagaaa
      241 gaagaagcaa aaaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnngtataaa ttcagatatg gatgaatatt ttccttctat tacagtagat
      361 agtaaacgaa tgttatttac acgaaagatt aagagcgata tttacgaatg gcaggaagat
      421 ttttatataa gcactttgca gcaggacagc agttggggta aggcaaaaaa tgtgggtgca
      481 ccggtaaata cagagttaaa tgaaggagca ccatcataca gtgctgatgg ccaactgata
      541 ttttttacgg catgtgaccg acctgaaagt aaaggttcat gtgatattta ttactccaga
      601 aaatttggtg atgtatgggg aaagccaatc aatcttggct cgccagtgaa cacaggtgca
      661 tgggaatcgc aaccttcttt ttcgagtgat ggcaagacct tatactttgt tcgtggcagc
      721 aacagccgca ccaacgatca ggacattttt atgagtgagg tgagtgaaaa aggatggagt
      781 acaccggtga aactaaacaa caacatcaac acagcaggaa aagaagaagc cgtatttatt
      841 cacccggata atcaaacgtt atattttgtt tctgacggat ggccgggcat gggaggctca
      901 gatatcttta tgagtaaacg ccaggctgat ggtcagtggg gcaaagcagt aaacttagga
      961 tatccaataa atacatttgc caatgaagca ggattgattg taagtccttc gggcgataaa
     1021 gcctatttca gcagcaacag agaaggtgga ttaggaggtt tggatattta cagtttcgac
     1081 ttgtatgata aaattaaacc agaccctgtg agttatgcaa taggatatgt gtatgatgat
     1141 gaaacaaaaa aacctgccga tgcaaaattt gaaatcattg atattgaatc gggcaagaca
     1201 gttgtagaat cttattctga caaaaacaca ggagagttta tggtgacctt gccttatgga
     1261 aaaaattata tgcttaatgt gtcgaagcga aaatatcttt tctactccga tcattttgaa
     1321 atggataaac catcggatat taaaaatgca aagcagttga tagtaagact taaacccgtg
     1381 aaagatggca gcaccatggc tttgaataat attttctttg acaccaattc tgcagaacta
     1441 aaacctgaat caaaaacaga gttaaataaa ctggtgagtt ttattaaaag taatgccatt
     1501 gcaaaacttg aaattggagg gcatactgat aatgtaggtg atgacaaagc aaacatgctg
     1561 ttgtctgaca agcgagcaaa atcggtttgt gattatctta ttacatcagg cattgaagca
     1621 tcgcgattga tttccagggg ttatggagaa acaaaacctg tgtcagataa tacaactgag
     1681 gaaggaaagg caaaaaacag aagaacagaa atcactatat tatcagttca ttaatttttt
     1741 tgcatacaaa atttttctgt taaatacgtg ttgaaaatta tttaaaaata ttttcggaga
     1801 agggggcgtg gcaattgact aaatttgctg catggaagaa aatgtggaag caggcaggag
     1861 tgcaaaaaag aaagtagctc ccaagtcgtt aacaaatcaa cagattgcat taggattagg
     1921 caaaattcca ccacaggctg tggatatgga agaagcagta ttaggcgcat taatgcttga
     1981 aaaaaatgca ctcactgctg ttatagatat tttacataca gaagctttct ataaagaagc
     2041 acatcagaaa atatttaaag tcatacatga tctttttcat aaagctgagc caattgatat
     2101 tttaacagtt acacatgctt tgaaaaaatc aggcgattta gaattagtag gtggtgcatt
     2161 ttatattacg cagttgacca acagaattgc ttctgcggct aacattgaat atcatgcgcg
     2221 gattatcctt caaaaattta ttcagcgtga gttgattcgt atttcgagtg aaactataaa
     2281 gagtgcttat gaagacacat cagatgtttt gaagatgctg gacgatgcag agaaaaattt
     2341 attttcaatt gcagaaggaa acattcgcag aaattacgaa aacatgaatt ctcttattac
     2401 caaagccatt agggaaatag aaattgcatc aaaacaaaca caaggcatta caggagtaga
     2461 aagtggcttt actgacttag atcgtataac agcaggatgg caaaaatctg atttgataat
     2521 tgttgctgca agaccgggta tgggaaaaac agcctttacc ctatcgttag cagccaatgc
     2581 cgcattacaa ttcaacagac cggttgcgtt tttctcattg gagatgtcaa gtgttcagtt
     2641 ggtaaaccgt ttgctttctt cacaggcaga aataacttca gaagccatca gaaaagggca
     2701 acttgcagaa catcaatggc aacagctaaa cagcaaaatc gctccgcttt ctgatgcacc
     2761 tatttttatt gatgataccc cggcattatc ggtttttgaa cttcgtgcca agtgcagaag
     2821 acttgttgca gaaaagaaag tacaactcat cattattgat tatatgcaat tgatggtggc
     2881 aggaacagac aatcgtaatg gaaaccgtga gcaggaaatc agtaccattt cgcgttcatt
     2941 aaaagctata gctaaagaat tagaagttcc gataatttgt ttgtcgcagt tgagtcgtga
     3001 agtagaaaag cgcggaacag cttctaagcg cccgcaattg agcgaccttc gtgagtcagg
     3061 agctattgag caggatgctg atatggtaat gtttatttac cgcccggaat attatggtat
     3121 ggatgtagat gatgaaggcc attcaacaaa aaatactgca gaaattatta ttgccaaaaa
     3181 cagaaatggt tctttaaata ccgttaaact cacctttatt ggtcaatata ctaagtttaa
     3241 agaccgtgag ctagaaaatc agacaccaca agataaagca atatatggct atactgatga
     3301 aagcaataag gttagaatac ctaatccgaa tgttgaagca gagaatcgtt acacacgtgg
     3361 ttcaaaaatg aacgacatgc ctgacgaaga tcctttttag tctgacttaa ttttctaatt
     3421 gactctttac aatttccaaa ccttgctgca aggtgtgagg ctgaaagttt aattcacgtt
     3481 ttgctttatc aatattaaaa cctgttagca aaggacgttt tgcaggttga ttgagttgag
     3541 cagtagtgat gggatgaata tattttttat ccagctgaaa aaaatctgct gtacggttta
     3601 ccatttcaag aatgctcata gtttcactgc cggatacatg atatattcct gttgcgcgtt
     3661 ttaatgcagc ctgcaggcag gcatcagcaa gatcttcggc aagtgtaggt gagcgcatct
     3721 gatcgctgat gacattgata tttttttgct gacgcagtga gtttattgtc cagataacaa
     3781 cattacttct ttgcttgtca tccactactc cataaatgat gattgtacgt aaaatagcag
     3841 aatctatttc tgcttgctgc acaacttttt ccgattcaaa tttactgcgg gcataatgac
     3901 tcaacggatt gggggcatct tcttcgctgt agttgccctt ttcaccatca aaaacaaaat
     3961 cggtactcag atgaataaaa tgtatgtcat gaagttgaca cagttcagtc attgtcctga
     4021 ctgcttctac attcatcttc cagcaatttt cctgatcttt ttcgcaggca tcaacattgg
     4081 tcattgcagc agtgttgatg atacaatgcg gcttaaaatt ctcaacaatg gcagttactt
     4141 catcagcatt ggtaatatcc attgaaacat agtcatagcc tgcttttgta tgcaaacggt
     4201 tttcaccttt agaagttgca agcagttgta tgtcgctatg ctttatagct gcataaacaa
     4261 ttttctgccc cagcaatcca ttacttcctg tcaataaaat acgcagcatg acattgcaaa
     4321 attaaaacgt gagcgatgct cccaatgatg gcattattgg caattgattt accacattgt
     4381 gtctgatgcg atcaacataa aacacatttt tacgattata aacattgaca acacttgcca
     4441 ccacttccag ctcactgtgt cgggcaagat agaaaatttt cttgattgaa atatcgagac
     4501 gatgataggt cggtaatcgc gactcattta atggtccgta gagcacaccc atttcgccat
     4561 tgttctgagt atagtcagta gcaagtccat cctgaaaatt gagataagga taaaatccct
     4621 gatttaaagt tacaggaaaa cctgatccga aattccagcg ggcatcaact tcccagtcga
     4681 gttttttacc taacttataa gagccgatga tattgatatt atgtcgtctg tcgaaatgtg
     4741 gagcatattc gtagttgcct gtattgcgtg tgctgaatcc caaagaataa acgagccatg
     4801 catacattct tctgtattca tatttcaaaa caacgtcaac accttgtgta aatccttttt
     4861 cgatgataaa acttttctta acggagtcgg gcttgtcgaa gttgtttgca gtgtcatcat
     4921 aaaccttgtc gcggttgata ttggtaagct gattaaaatc tttaatgtag ccttcgatgt
     4981 taatatcaaa atgtttgctg atatcatatt caacaccaac aactgcatga cgtgcaagct
     5041 gcagtttttt cttaacggat tttccatcaa actcagaagg taaatcatca ggtgcagaaa
     5101 ggaaaccata gaaaaagttt accacatcgc ggtcgctgtt ggcagcaata agattttgag
     5161 catacagtcc accggcagct ttaagtctga tgttgtcgct caggttgtat ttaattccaa
     5221 ggcgaggttc aggagaaaat tcagagatgg atgcataata ctgcaagcga acggaaggat
     5281 caatgatgag tttattgatg atttttttgt agcgaaagaa gattccgatt tcagtagtgt
     5341 tttctgtctg attgatttgt ctcttggcat aattaaaata ttcgaaatcg gttttgaagc
     5401 cttgcacttc aataccataa ttaatttcgt cattatttaa aaagtaaagc cagttaaagc
     5461 ccatgttaaa tccattcact aagctggagc gtggtttttc ttcggcttct gtaagcttaa
     5521 ttttatagct ggagaaagcg aggttaccac gcagcaatac ttttgatgca ccgggcacca
     5581 acacaaagtt cattccacca ccggaagaac tccagtcgta ttttgctacc ttgctgtaat
     5641 ttactttatc ttgaaaatta aatccgaaaa agctaacctt acttccgctt gcactgttga
     5701 caaccacttt tccataaata tcattgaaag aatacggcaa gccgtcatcg gaagcatatt
     5761 tgtaaatact ttttgaagtc tggtcgaggt aggaattttt tactgaaaga atgaaagacg
     5821 ctgttccgtc attttcgtct ttagctttct ttatcggacc ttctaacaaa actttagaaa
     5881 ggaatgtgcc tgcagaaact tttccgccaa tacgtttttt atttccatcg cgtgtggtga
     5941 tgtccatcac tgatgaaatg cgtccaccat attgcgcacc aaaacctgca gtataaacat
     6001 cggtattttt taaaatgtct gtatcaaaca cactaaacaa gccaatgcta tgaaacggat
     6061 tgtagatgac cataccatcg agcaacactt tgttttgtac aggagttcct ccgcggatat
     6121 acaattgccc tccctgatcg ccactgctta caacaccggg cactacctgc atgtactgta
     6181 ccaaatcacc ttcggcacca agacttggca gagatttaat ttcctgaggt gttacagact
     6241 ctactgcaat tttaacttct gttttttttg tttgtctctc tgccgatacc tcaacctgtt
     6301 tcaactccac attttttttc t
//
LOCUS       DAORQT010000087         6320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2004_length_6320_cov_8.906145, whole genome shotgun sequence.
ACCESSION   DAORQT010000087 DAORQT010000000
VERSION     DAORQT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_6320_cov_8.906145"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..>284
                     /locus_tag="PK134_04410"
     CDS             11..>284
                     /locus_tag="PK134_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38122.1"
                     /translation="MAVSLKNIGSACDDMGNNSDALVYYGRALNIFSSIDDKSGVSAC
                     YNNMGLVYRTLGNFSEALKNHFASLKIKEAIGDKNGIALSYNNIGAV"
     assembly_gap    285..294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <295..1857
                     /locus_tag="PK134_04415"
     CDS             <295..1857
                     /locus_tag="PK134_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38123.1"
                     /translation="QKNYDEALKNHRAALAIRLSLGNKRGIASSYGNIGNVYQAQGNN
                     TEALKNYTEALKIAEETGDKVGMTTALNNMGAAYFEMGDYENALQHNTDALRIAEEAG
                     DLSSIAAASINTGNVMVQMKQPQAATEWLQKALEIGKEIGEVEVIKNSYEGLSQADSA
                     LGKYSDALQHYKMFIRYRDSIVNEENTLKTVQLQMQYEYDKKDAIARAVQEKKDEVAQ
                     QEVQKQKLLRNSFIAGFALMLALAAVSYRSFVRKRKDNHLIEKQKLLVEEKNKEITQS
                     LHYARHIQAAVLPNQKNIAALLKNHFILYLPKDIVSGDFYSVLQNKDKVIVAAADCTG
                     HGVAGAFMSMIGTSLINHLVNEKNITNPAVILDELNEGIINSLKQRQGDSNDGMDIGL
                     CSIDFKENKLLFAGANRPLWLFRNEEIKVIKPDKFPIGGMQVDHKNKYTAHSIDLLPN
                     DTIYIFSDGYSDQFGGEQGKKMMSKRFKQYLQVMQHLPMDKQKEQLEQLFNSWKGNHE
                     QVDDVLVVGIRV"
     gene            1978..6153
                     /locus_tag="PK134_04420"
     CDS             1978..6153
                     /locus_tag="PK134_04420"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38124.1"
                     /translation="MFKNLFFSKGVKSIALAAFCLLTFTLGANAQILAWEFNGAAGNE
                     TTIAATTLDANLNSSSVSRGSGVNASGLTNAFASTDYTVSGTFANAVTNNEYQQFSVN
                     AKSGYKVSLSTLDVNFRRSNTGPNTFQWQYSLDGFATAGINIGSAISYTTSPTNGTAQ
                     SQIDLSVISALQNVSNATTITIRLYGYGASAGTGTFALGRLTGNDLAIGGTVDPDGGS
                     PVLTVGTLSNQFGNVCIGTTTNYETFTVSGTDLDGSSVDVGTLSGFTYCLTPGGTYTS
                     TLNLPYTAPTLSSTTVYVKFSPTLVQSYAGSIPVTGGGASAGSCSVSSASGINTSPSL
                     TTGSATGITQTTATCEGTITDNGCTSVSAYGIEYSTTNNFTPGTGTQVSSSNISGNTY
                     TSALTGLAGNTTYYYRAYATNTAGTGYGAQASFTTDAFTAPVATAATNIGIHSFRANW
                     NSVSGATGYFLDVSTSSTFLAPVTTDLVYWDFPNDPDDATADGGILSNLSATISSTAG
                     GTMTFSTNGASTKSASINNWDNGNGTKYWEVSFDATGYYNLAFSSKQQSSNTGPRDFK
                     VQYKIGSGGTWTDVTGSSVTVANNFTSGVLTDIALPAACDNQSSVYLRWIMTSNTSVG
                     GGSVAATGTNRIDDLFVTGYEGDFVLENQSVAGLYYDVTGLNPITTYYYRVRATDGVA
                     TSDNSNVINLTTLDVCDLFTVTASSNAPICSSTNLNLNANASGSDGTITGYEWSGPGG
                     YSNMTQNPSVSNPLAGTYTVTVTDDNGCTVTDNVSATVEAAPTANAGSDDIICSSGSA
                     ALNGIIGGDATGGTWTTAGDGTFSPNATTLNAAYVPGTTDISNGSVVLTLTSTGSVLC
                     SAVNDQVTITIQTGVPSQPAVVSGAPTSVCPPAGPYTLTTSSTNANSYNWYVVGANTG
                     ITFLSANGSNSMDVQYGVTANSSYSVRVEASNACGTSSYRSAFTRRSVSTPATVSGNK
                     VACATTSDTYTCTAVAGAESYQWSITGDATVSGTTNSVTVNFGPAWTGGTLCVSSQVG
                     CFTSPSKCMTITSSTPAVGAISGSATACPNGVVSFSVAPLAGIQTYNWTTPPNSSVTL
                     GSGTNNVQVTFNGSYNGVGNICVSATSICGVTTAPKCKTVAPGLPSVPASITGGTNGL
                     CNQSGNFSCPLQAGNTYNWTAPGTINNNGSNSVNIQFSNITTGSVCVSASNSCGSSAY
                     RCVTVKGAPNSPVALTSNPAVICANTTGVEFNANTSNTTGAYTLTWSYTPAAATYNSG
                     GGNTNQLILDWGNTNGVVVVTASNACGSGAKSYTVNLGCRQQGQDDVAALNIYPNPAS
                     GTVNINLNAGNQQAVQCIITDLSGKVVMQQTYNVAVGNNTFSADVSHLAKGIYMLQIA
                     GNTMQATQKLIIQ"
BASE COUNT         1964 a         1335 c         1292 g         1719 t
ORIGIN      
        1 gaaaagaggc atggctgttt cactaaaaaa tattggcagt gcctgcgatg atatgggcaa
       61 caacagcgat gcattggttt actacggacg ggcattaaat attttcagct ctattgatga
      121 taagtccggt gtttctgctt gctataacaa tatgggtttg gtgtacagaa cacttggcaa
      181 tttttcagaa gcgttaaaga accattttgc atctttaaaa attaaagagg ccattggcga
      241 taaaaacggc attgcacttt catacaacaa cattggtgca gtgtnnnnnn nnnncaaaaa
      301 aattatgacg aagcattaaa aaatcatcgt gcagccttag ccataagatt gtctttgggc
      361 aacaaaagag gtattgcctc atcctatgga aacataggca atgtctatca ggcacaaggc
      421 aacaacaccg aagcgctgaa gaactatacc gaagcactta aaatagcaga agaaaccggt
      481 gataaagtag gtatgacaac agccctcaac aatatgggtg cagcctattt tgaaatgggc
      541 gactatgaaa atgccttgca acacaatact gatgccctac gcattgctga agaggcaggc
      601 gacctttctt caattgctgc tgcaagcatc aacaccggca atgtcatggt gcagatgaaa
      661 caaccacagg cagcaactga atggcttcag aaagcattgg agattggtaa agaaattggc
      721 gaagttgaag ttataaaaaa cagttacgaa ggactcagtc aggccgacag cgctttggga
      781 aaatacagcg atgcactgca acactacaaa atgtttatcc gctatcgcga cagcattgtt
      841 aatgaagaaa acacactcaa aaccgtacag ttacaaatgc aatatgagta tgataaaaaa
      901 gatgccattg cccgtgctgt tcaggaaaag aaagatgaag ttgcacaaca ggaagtgcag
      961 aaacaaaaat tactcagaaa cagttttatt gccggatttg cattgatgct tgcccttgct
     1021 gccgttagct acagaagctt tgtgcgtaaa cgaaaagaca accatctcat cgaaaaacaa
     1081 aagttactcg tagaagaaaa aaataaagaa attacgcaaa gtctccacta tgcacgacac
     1141 attcaggctg ctgttttacc taatcaaaaa aacattgcag ctttactgaa aaatcacttt
     1201 atactttact tacctaaaga tattgtaagc ggagattttt acagtgtatt gcaaaacaaa
     1261 gacaaggtaa tagtagctgc tgccgactgt accggacatg gtgtggcagg cgcttttatg
     1321 agtatgattg gtacttcgtt aataaaccat ctggtaaacg aaaaaaacat taccaaccct
     1381 gcagttattt tagacgaact gaatgaaggc atcatcaact ctttgaaaca acggcagggc
     1441 gattctaacg atggcatgga cattggcttg tgcagcatag attttaaaga aaataaattg
     1501 ctttttgcag gtgccaaccg tccgctttgg cttttcagaa atgaagaaat aaaagtaatt
     1561 aaacccgata aatttccgat tggaggcatg caggttgacc ataaaaacaa atacactgca
     1621 cacagcatag atttattgcc taacgacacc atttatattt tttctgatgg ctattccgat
     1681 cagtttggtg gcgaacaggg taaaaaaatg atgagtaaaa ggtttaagca atacctgcag
     1741 gttatgcagc acctgcctat ggacaaacag aaagaacagc ttgaacagct ttttaattca
     1801 tggaaaggca atcacgaaca ggttgacgat gtattggttg tgggcataag agtgtgatgt
     1861 tgcggcactt aaaaaatgtt aaaaactaaa tttttcaatt aaagccgttg ttttattttt
     1921 gcaacgttgc caatagcaac aaagaactta ttttgtaaat cattaaaacc aaatattatg
     1981 tttaaaaatt tattcttttc gaagggtgta aaaagtattg cccttgcagc attttgtttg
     2041 ctgactttta ccctaggtgc caatgcacag attttagcat gggaatttaa tggagcagca
     2101 ggaaatgaaa ctaccattgc agccaccaca cttgatgcta atttgaacag ttcatcagtt
     2161 tcaagaggta gcggtgttaa tgcttctgga cttactaatg cttttgcttc aaccgattac
     2221 accgtaagcg gaacttttgc taatgcagtt acaaacaacg aatatcagca gttttcagtt
     2281 aatgcaaaat ctggctacaa agtgtctctt tcaacattag atgttaattt cagaagatcc
     2341 aatacaggtc ctaacacatt ccaatggcaa tatagtttgg atggttttgc aacagcaggt
     2401 attaatattg gttctgcaat tagttataca accagtccaa caaatggaac tgcgcaaagt
     2461 caaatagatt taagtgtaat ttctgcttta caaaacgtaa gtaatgcaac aactattact
     2521 attagacttt atggttatgg ggcatctgca ggaacaggca cttttgcgct aggaaggctg
     2581 acaggtaatg atcttgcaat tggaggaact gttgatcctg atggtggcag ccctgtactc
     2641 actgtcggca cactctctaa tcaattcggc aatgtttgta ttggtactac aacaaactac
     2701 gaaacattta ctgtttccgg tacagacctc gatggttcaa gcgtagatgt tggtacatta
     2761 agcggattta catactgcct tactcccggt ggaacatata cttcaacact caatctgccc
     2821 tatacggcac ctacattaag cagcactact gtttatgtta aattttcgcc aacgctcgtt
     2881 caaagctatg caggctctat tcctgtaacc ggtggtggcg catctgccgg cagttgttct
     2941 gtttcatctg catcaggtat taatacttca ccttcactca ccacaggcag tgcaacaggc
     3001 atcacacaaa ctactgccac ctgcgaagga actattaccg ataacggatg tactagcgtt
     3061 tctgcctatg gcattgaata tagcactaca aacaacttta ctcccggaac aggcacacag
     3121 gttagcagca gcaacatcag tggcaacacc tacacttctg cactaacagg tcttgcgggc
     3181 aacacaacat actactatcg tgcctatgca accaacactg caggcacagg ctatggtgca
     3241 caagcatcat tcacaacaga tgcctttact gcacccgtag caacagcagc taccaacatt
     3301 ggcattcatt ctttccgtgc caactggaac agtgtttccg gtgccacagg ttatttttta
     3361 gatgtttcta cttcttcaac atttttggct cctgttacaa ctgatttggt gtattgggat
     3421 tttccaaatg atcccgatga tgctactgcc gatggtggaa tattaagtaa cctgtctgca
     3481 acaatttctt ctactgccgg tggcactatg acgttttcta ctaacggagc ttcaacaaaa
     3541 agtgccagta taaacaattg ggataatggt aacggcacca aatattggga agtaagtttt
     3601 gatgcaacag gatattataa ccttgcattt tcaagcaaac aacaatcttc aaataccggt
     3661 cctcgcgatt ttaaagtgca atacaaaatt ggcagtggtg gcacatggac agatgtaaca
     3721 ggcagttcag ttactgtagc taataatttt actagcggtg tattaacaga tattgcctta
     3781 cctgcagcat gcgacaacca aagcagtgtg tatctgcgtt ggattatgac aagcaatact
     3841 tctgttggtg gtggttcggt tgcagctacc ggcaccaacc gtattgatga tttatttgtt
     3901 accggctacg aaggtgattt tgttttagaa aaccaaagcg ttgcaggttt atattatgat
     3961 gtaacaggtc tgaatcctat cacaacttac tactaccgtg ttcgtgctac tgatggtgta
     4021 gctacttcag acaacagcaa tgtcattaac cttactacct tagatgtttg cgatttgttt
     4081 accgtaacag catcaagcaa tgcaccaatc tgttcaagca ccaaccttaa ccttaatgcc
     4141 aatgcaagtg gttccgatgg aactatcacc ggctatgagt ggtcaggtcc cggtggctac
     4201 agcaacatga cacaaaatcc ttctgtaagc aatccgcttg caggaactta tacagtaacc
     4261 gtaactgatg ataatggatg tactgtaaca gacaatgtat ctgcaaccgt agaagcagca
     4321 cctactgcca atgcaggaag cgatgatatt atttgctcca gcggcagtgc agcattaaat
     4381 ggtataatcg gtggcgatgc taccggtgga acatggacaa ctgctggcga tggaactttc
     4441 tctccaaatg caacaacatt aaatgctgct tatgttcccg gaacaacaga catcagcaat
     4501 ggcagtgtag tgcttacact cacttcaaca ggcagtgtgt tgtgtagtgc agttaacgat
     4561 caggtaacaa ttaccattca aacaggtgtg ccttcacaac ctgcagttgt ttcaggagcg
     4621 ccaacaagtg tttgtcctcc tgcaggtcct tatactttaa caacatcttc aactaatgcc
     4681 aatagctaca actggtatgt tgttggtgcc aacacaggaa taacattttt atctgccaat
     4741 ggcagcaact caatggatgt gcaatacggt gtaacagcca acagcagcta ctctgttcgt
     4801 gttgaagcaa gcaatgcctg cggaacaagc agctatcgtt ctgcatttac acgcagatca
     4861 gtttcaacac ctgctactgt tagtggaaac aaagtagctt gtgccaccac cagcgatact
     4921 tacacttgta ctgccgttgc cggtgcagaa tcctatcaat ggtctatcac tggcgatgcc
     4981 acagtttcag gaactaccaa cagcgtaact gtaaactttg gtccggcatg gacaggcggt
     5041 acactttgtg tttcatcaca agtaggttgt ttcacaagcc catcaaaatg tatgactatt
     5101 acttcttcta ctcctgcagt tggtgccatc agtggcagcg ctactgcatg tcctaacggt
     5161 gtagtgtcat ttagtgttgc tccgcttgca ggcattcaaa cctacaactg gacaacgcct
     5221 ccaaactcat cagtaacttt aggtagtggt acaaacaatg tgcaggtaac atttaatgga
     5281 agttacaatg gtgtcggaaa tatttgtgtt tctgctacca gcatttgtgg tgtaactaca
     5341 gcgcctaaat gtaaaactgt tgctccgggt cttccttcag ttcctgcttc aatcacaggc
     5401 ggaacaaatg gtttgtgtaa ccaaagtgga aacttcagtt gtccattgca agccggaaat
     5461 acttataact ggactgcacc gggcacaatt aacaacaatg gttcaaacag tgtaaacatt
     5521 cagttcagca atataactac aggaagtgtt tgcgtttctg catccaatag ctgcggtagc
     5581 agtgcctatc gttgtgtaac tgtaaaaggt gcacccaact ctccggtagc acttacaagc
     5641 aatccagcgg ttatctgcgc caacactacc ggtgttgaat ttaatgccaa cacttcaaac
     5701 acaaccggtg cctatacatt aacatggtct tatactccgg cagcagcaac ctacaatagc
     5761 ggtggtggaa acacaaacca acttattctt gattggggaa atacaaacgg tgttgtagtg
     5821 gttacagctt ctaatgcctg tggcagtggt gctaaatcat acactgtaaa cctaggttgc
     5881 cgtcagcaag gtcaggacga tgttgcagcg ttaaacatct atccaaatcc tgcaagcgga
     5941 acagttaaca tcaacctcaa tgctggcaat cagcaagctg ttcaatgcat catcaccgac
     6001 cttagtggta aagttgtgat gcagcaaaca tacaacgtag cagttggcaa caacaccttc
     6061 tctgccgatg tttcacacct tgcaaaagga atctacatgt tgcaaattgc aggcaacaca
     6121 atgcaggcaa cacaaaaact aattatacaa taacggtttt gcactaagca taaatcaaat
     6181 caaaaggctg tctcacaaag gcagcctttt tttgtggtag aataaagtgg ttgcaataat
     6241 tattaactta ctaaaatgta atatcaaata gttcaaaaac taattttatc tttgttcact
     6301 aattgaatga tatgttagag
//
LOCUS       DAORQT010000088         6273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2035_length_6273_cov_8.825346, whole genome shotgun sequence.
ACCESSION   DAORQT010000088 DAORQT010000000
VERSION     DAORQT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_6273_cov_8.825346"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PK134_04425"
     CDS             <1..1121
                     /locus_tag="PK134_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38125.1"
                     /translation="VTIMGYAGICGSDDLAARSIAYFHTKSFDEIVDYSTLSTGNSCP
                     VNTITFNNAPTVSGGGIFNVPVGTAFKLNGYGSDPDGDSITFSWEQFDLGPAGAWSLP
                     TGNAPMYRSYAPSTSALRLFPKLSVLLNQVNAKGELITSYARSLHFRLTARDNKPNGG
                     GVTYNDTVTTVNVVQTSAPFAITYPNVTGISWPALSTQTITWEVGGTDAAPFNEQFVN
                     IYLSINNGTTFPITIATNVANNGSYTFTVPNNPSNICRMWVEGASTGSIFFDINNKVF
                     TITAPVGINEVNSLSQLTTYPNPADNELLVYLGNNYHGQLNISLYSVNGQLVLSQTIA
                     AKNATAQVALNVEQLPAGLYFLKAETENGTAERKVMIK"
     gene            1238..4543
                     /locus_tag="PK134_04430"
     CDS             1238..4543
                     /locus_tag="PK134_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38126.1"
                     /translation="MKPFFKAVFTLLFTFIILHSADAQFYQGSQMSFGKNRVQYTDFY
                     WTFFRFKNYDVYNYVGGKELATYVAEQAQNEMDVMERTLDYRSSSRFQIVIFNKLSDL
                     KQTNIGLELDDQAYNIGGMTKVIGNKVLIYFDGNHQHLQEQIRAGMARALLEQLFYGG
                     NIRERLQASILLNLPQWYTQGLVTYLSKGWTPEDDNKLRDYIMTEKKPVLNKLIERDE
                     VFAGQSLWHYIITTYGETSLSNLVYMTRINRSLESGFVFVLGVRLKQLSADWLSYHTK
                     KYQEFEKNKFPIAATPLKVKEKNKIIYQVKTDPFGNRVSYVTNKRGRYKVKITDVKTG
                     KRTCADKGGYVSLTTENDRSFPITAWHPSGKILAIVKEQKGNIILNLFNTETKKSDFS
                     EIMSFSKILNMTYSPDGSKLLMSAVQKGQSDIYVFNVRGRNFDQITNDVYDDLYPQYD
                     ITGNKIIFSSNRSNDTLGVDVKQNTPQNNFDLFMADLYNREGNLLRITHSPENETFPV
                     MSDSLRISYLSDKNGVINRFVSEMDSTISFIDTTEHYRYFFRHYALTNSNRNILEQDF
                     SARKNKFTELAVNNGRYRIYLKDNMPFDKSKTFDLSYIAQPVFQPKQENKPKVAETPT
                     RVVTIEPSQKKDSAAIDVGNYVFQSEFKSKKKKEEPIENKSVATDTTKIVQEQVKLQP
                     DSNDVKSTPDSAIFRMPKQRNYDISFSSDYILTQLDNSLFNTTYQPYTGGAVYFDPGM
                     NALFKIGMSDLMNDYKISGAFRIGFDFNSNEYMLMIESLKKRIDKQILFSRQSKLFSV
                     SDLDVLRTHTYNLKYSGKYPFSEISAVKGTIDARMDKTIYVATEKAQLEKSDITNYWA
                     SIKGEYIFDNTINKALNLYNGTRFKIFAEVFRKINKPEKMVYIIGADFRHYIKVHRQI
                     IWANRLSASTSLGKQKLIYYLGSQDNVIVPGNNFNNEISVDKNEEYVFQTLASPMRGF
                     KQNIRNGSSFALFNSELRIPVFQYLLNRPIRSDLIRNFQAVVFFDAGTAWSGTSPFSD
                     KNGLNEEVIGGGGSNPVTVTVIKQIQPVVGAYGFGFRSRIFGYFIRADWGFGIDDRQV
                     QKPLFNLALGLDF"
     gene            complement(4544..5806)
                     /gene="rodA"
                     /locus_tag="PK134_04435"
     CDS             complement(4544..5806)
                     /gene="rodA"
                     /locus_tag="PK134_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRB38127.1"
                     /translation="MRKQKSVFQNIDWLLVLLYLVMVIAGWLNIFAAVYNEEHSVITD
                     FSQNYGKQMMWILTSLVIAFFILIIDGKFYDAFAGVIYAISIISMIAVLFFGHTVAGS
                     KSWFRFGDFGIQPAEFAKFATAMMIAKFLSPMGLKKNTWRTRIYAVAIFFIPLLLILL
                     ENETGVALVFLSFCIVLYREEIIPGWLLMIGISAAVLFVVSLLVPKMILVTSLLVLAA
                     LIVLLFRKWKTFFVVAGLVAICIGVVVSVDHVFNHVLEPHQKKRIQVFLGMEEDLKGA
                     GYNVNQSKIAIGSGGLTGKGFLKGTQTKYNFVPEQSTDFIFCTVGEEWGFLGTTTVIL
                     LFLALLYRIIFIAERQKSAFARIYGYGVAGVIFFHFMVNVGMTIGLMPVIGIPLPFFS
                     YGGSSLWGFTILLFILIKLDSYRTFVLR"
     gene            complement(5810..>6273)
                     /locus_tag="PK134_04440"
     CDS             complement(5810..>6273)
                     /locus_tag="PK134_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRB38128.1"
                     /translation="IVANKGYYYVPHIVKEIGYKHYLPELFKKKNYTLVDPKYFDAIQ
                     DGMEWVFERGTARGSRVDSLVMCGKTGTAQNPHGKNHSVFFCFAPRENPKIAVAVLVE
                     NAGQGAHFAAPIASLMVQKYLLRTIKRPDLEKRMLDAVIVPMANDSTVLDD"
BASE COUNT         2131 a         1156 c         1114 g         1872 t
ORIGIN      
        1 gtgtaaccat tatgggttat gcaggaattt gtggctctga tgatttggct gcacgcagta
       61 tagcttattt tcatacaaaa agttttgatg aaatagttga ttattcaacc ttgtcaactg
      121 gcaactcttg tcctgtaaat accatcacgt ttaacaatgc acctactgtt agtggcggag
      181 gcatatttaa tgttcctgta ggcacagcat ttaaattaaa tggctatggc agcgaccctg
      241 atggtgattc tataactttc agttgggagc agtttgactt aggccctgcg ggagcatgga
      301 gtttgccaac aggtaatgca cctatgtatc gcagttatgc tccttcaact tctgcacttc
      361 gtctttttcc aaaattgtct gtattgctca atcaggtaaa tgctaaaggt gagttgataa
      421 catcttatgc acgctcatta catttcagac ttactgcaag agataacaaa cccaatggtg
      481 gtggcgtaac ttataacgat acagttacaa cagtaaatgt tgttcagact tctgcacctt
      541 ttgcaattac ttatccaaat gtaacgggta tatcatggcc cgcattaagt acacaaacaa
      601 ttacatggga agtgggtggc accgatgctg caccatttaa cgaacagttt gtaaacattt
      661 acctttcgat aaataacgga acaacattcc ccataacaat tgcaactaat gttgctaaca
      721 atggcagcta tacttttact gttccaaata acccatcaaa tatttgcaga atgtgggttg
      781 aaggtgccag cacaggttct atattttttg atattaacaa caaagtattt accattacag
      841 cacctgttgg aattaatgaa gtcaatagcc ttagtcagct tactacctat ccaaatccag
      901 cagacaatga gctgttggtt tatttaggca acaattatca tggtcagcta aacataagtc
      961 tttatagtgt taacggacag cttgtgttgt cacaaactat agcagctaaa aatgctacag
     1021 cacaggtagc tttgaatgta gagcaactcc ccgcaggttt atattttctg aaagcagaaa
     1081 cagaaaacgg gacagcagag cgtaaagtga tgataaaata atatcccgat aattcatgaa
     1141 agggttgttg aataaacagc ccttttttta tgttttaatt ttcggtttaa ataaagttca
     1201 aattaactac tttcgcatat cacataaaac aaaaaaggtg aagccatttt tcaaagccgt
     1261 attcacgctc ttatttactt ttataattct acattctgct gatgcacagt tttatcaggg
     1321 ctcgcagatg tcgtttggca aaaacagagt acaatacaca gatttttact ggacattttt
     1381 cagatttaaa aattacgatg tttataatta cgtaggcgga aaagaattag ccacctatgt
     1441 tgctgaacaa gcacagaatg aaatggatgt tatggaacgc actttggatt atcggagttc
     1501 gtcacgattt cagattgtaa tatttaataa actcagtgac ctcaaacaaa ccaatattgg
     1561 ccttgaatta gatgatcagg cttacaatat tggaggaatg acaaaagtaa taggcaacaa
     1621 agtgcttatt tattttgatg gcaatcatca gcatttacaa gaacagattc gtgccggcat
     1681 ggcaagagcc ttacttgaac aattgtttta tggaggaaac atcagagaac gattacaagc
     1741 ttctattctt cttaatcttc cacagtggta cacacaaggc ttggtaacct atctttcaaa
     1801 aggatggaca cctgaagatg ataacaagct gcgtgattac atcatgacag aaaaaaaacc
     1861 ggtcttaaat aaactcattg agcgtgatga agtttttgca ggtcagtcgc tttggcatta
     1921 cattattaca acttatggag aaacttcatt aagtaatttg gtttatatga cacgcattaa
     1981 ccgcagtctc gaaagcggtt ttgtttttgt gcttggtgta cgtttaaaac aactttctgc
     2041 cgactggtta agttatcaca caaaaaaata tcaggagttt gaaaagaata aatttccaat
     2101 tgcagcaaca ccattaaaag ttaaagaaaa gaataaaata atttatcagg taaagacaga
     2161 tccttttgga aacagagtaa gttatgttac taacaaacga ggccgctata aagttaaaat
     2221 tacggatgta aaaaccggta aacgtacttg tgctgataaa ggcggatatg tttcacttac
     2281 tacggaaaac gaccgatcat ttcccattac agcatggcat ccgtcaggta aaatattagc
     2341 tatcgttaaa gagcaaaaag gaaatattat tctgaatttg tttaacacag aaacaaagaa
     2401 gtctgacttt tcagagataa tgagcttttc aaaaattctc aacatgacct attctcctga
     2461 tgggtcaaaa ctattgatgt ctgcagtgca gaaagggcaa agcgatattt atgtatttaa
     2521 tgtgcgcgga cgaaatttcg atcagattac aaacgatgtg tatgatgact tatatccaca
     2581 atatgatatt acaggcaaca aaataatttt ttcttcaaac agaagcaatg ataccttagg
     2641 tgttgatgtt aagcaaaata cgccacagaa caattttgat ctttttatgg cagacctcta
     2701 caatagagaa ggaaatttat tgcgcataac ccattcaccg gagaatgaaa ccttccctgt
     2761 tatgagtgat tcgcttcgga tttcttatct gtcagataaa aacggtgtca tcaatagatt
     2821 tgtatcggag atggatagca ccataagttt tattgataca acagagcatt acagatattt
     2881 tttcagacat tatgctttaa ccaatagcaa cagaaatatt cttgaacagg atttttcggc
     2941 aagaaaaaat aagtttacag aactagcagt gaacaatggc cgttacagaa tttacttgaa
     3001 agacaatatg ccttttgata aatcaaagac atttgatttg agttatatag cacaacctgt
     3061 ttttcaaccg aaacaagaga ataaaccaaa ggttgctgaa acacccactc gtgttgttac
     3121 tattgagcca tcgcagaaga aagacagcgc tgctatagat gtaggcaatt atgtttttca
     3181 aagtgaattc aaatcaaaga agaagaaaga agaaccaata gaaaataaat ctgttgctac
     3241 agatactacc aaaattgtgc aggaacaagt aaagttgcaa cctgatagta atgatgtaaa
     3301 atctactccc gactctgcaa ttttccgaat gccaaagcaa cgtaactacg atatttcatt
     3361 ttcatcagac tatattctca cacagctcga caacagttta ttcaacacta cctatcagcc
     3421 ttacacaggt ggtgctgttt attttgatcc gggtatgaat gcattgttca aaatcggtat
     3481 gagcgatttg atgaacgatt acaaaatcag tggagcgttt cgcattggct tcgatttcaa
     3541 cagcaatgaa tatatgctga tgattgaatc tttgaaaaaa agaattgata agcagatact
     3601 tttttcacgt caatctaaat tattttctgt ttcagacctt gatgtgttac gtacacatac
     3661 ctataattta aaatattcag gtaagtatcc tttcagcgaa atttctgcag ttaaaggaac
     3721 gattgatgca cgaatggata agactattta tgttgcgaca gagaaggcac aattagagaa
     3781 aagcgatata acaaattatt gggcaagcat taaaggagaa tatatttttg ataacactat
     3841 caataaagca ttgaatcttt ataatggtac acgattcaaa atttttgctg aagtgtttcg
     3901 taaaatcaat aagcccgaaa aaatggttta catcattggt gcagattttc gtcactatat
     3961 taaagtacac agacaaatta tctgggccaa ccgtttatcg gcaagtacat cattaggtaa
     4021 gcaaaaatta atttattatt tgggttcaca ggataatgtt attgttccgg gaaataattt
     4081 taataatgaa attagtgttg acaaaaacga agaatatgtt tttcagacat tagcttcgcc
     4141 tatgcgtggt tttaaacaga atatccgaaa tggtagcagt tttgcattgt tcaattctga
     4201 attgcgtatt ccggtatttc aatatttgct caacagacca attcgttctg atttgattcg
     4261 taattttcag gcagtagtat ttttcgatgc gggtactgca tggtcgggca catcaccatt
     4321 ttctgataag aacggattga atgaagaagt aattgggggc ggaggcagca atcccgtaac
     4381 cgttactgtg attaaacaaa ttcaacctgt agttggtgct tatggctttg gatttcgtag
     4441 tagaatattc ggttatttta ttcgtgccga ctggggtttt ggaattgacg atcgtcaggt
     4501 gcagaaacca ctatttaatc ttgcacttgg actcgatttc tgattatcgc aaaacaaatg
     4561 tacgatagct gtctaatttt atcagaataa ataagaggat tgtaaatccc cacagtgatg
     4621 agccaccgta actaaaaaag ggtagaggta ttcctattac cggcatcagg ccgattgtca
     4681 ttccaacatt taccataaag tgaaagaata ttactcctgc cacaccatag ccatagattc
     4741 ttgcaaaggc cgacttctgc cgttctgcaa taaaaataat tctatataac aatgccagaa
     4801 acaatagtat aacagttgtt gtaccaagaa acccccattc ttcgccaacg gtacaaaaaa
     4861 taaaatcagt actttgttca gggacaaaat tgtatttggt ttgtgtgcct tttaagaaac
     4921 ctttgcctgt taaaccacct gaaccaatag caattttact ttgatttaca ttgtaacctg
     4981 cacctttaag gtcttcttcc attcctaaaa atacctgaat acgttttttc tgatggggtt
     5041 ctaaaacatg attaaacaca tgatcaacac ttaccacaac accaatacaa atagcaacca
     5101 atcctgcaac aacaaaaaat gttttccatt tacgaaatag caaaacaatt agtgcagcaa
     5161 gtaccaacaa agaggtgacg agtatcattt ttggtactaa caatgaaacc acaaacaata
     5221 cagcagcaga aataccaatc atcagcaacc atccgggaat aatttcttcg cgataaagaa
     5281 caatacaaaa tgacagaaac accaatgcca caccggtttc attttctaat agaatgagca
     5341 gaagtggaat aaaaaaaatt gcaactgcat aaatacgcgt tcgccaggtg tttttcttta
     5401 accccatagg acttaaaaat tttgcaatca tcatggccgt agcaaatttt gcaaactcag
     5461 caggttgaat tccaaagtca ccaaaacgaa accacgactt actacctgca acagtatgac
     5521 cgaaaaataa tacagcaatc atagagatta tacttattgc ataaatgaca cccgcaaatg
     5581 catcataaaa ctttccatca ataatcagaa taaaaaatgc tataacaagt gatgtaagta
     5641 tccacatcat ttgtttgcca tagttttgcg aaaagtcagt tattacacta tgttcttcat
     5701 tataaactgc agcaaaaatg tttagccaac cggcaataac cataactaaa tatagcagta
     5761 ccagcaacca gtcaatattc tgaaaaacac ttttctgctt cctcattttt tagtcatcta
     5821 aaacagttga atcatttgcc attggaacta tcactgcatc taacattcgt ttttcaagat
     5881 caggacgttt tattgtcctc agtaaatatt tctgtaccat caaacttgca ataggtgcag
     5941 caaagtgtgc accctggccg gcattttcaa ctaaaacggc aactgcaatt tttggatttt
     6001 cgcgtggagc aaaacaaaaa aacacagagt ggttttttcc atgcggattt tgtgcagttc
     6061 ctgttttgcc acacatcacc aaactgtcaa cacgagaacc acgggcagtg ccacgctcaa
     6121 aaacccattc cataccatct tgtatggcat caaaatattt tggatcaacc aacgtatagt
     6181 ttttcttttt aaaaagttcg ggcaggtaat gcttatatcc gatttctttt acaatatgag
     6241 gcacataata atatccttta ttggctacaa tac
//
LOCUS       DAORQT010000089         6263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2039_length_6263_cov_6.336501, whole genome shotgun sequence.
ACCESSION   DAORQT010000089 DAORQT010000000
VERSION     DAORQT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_6263_cov_6.336501"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK134_04445"
     CDS             <1..459
                     /locus_tag="PK134_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRB38129.1"
                     /translation="VRNKIPDIAIRTTFISGYPGETEQDHETLIRFLEEMRFERVGVF
                     TYSHEENTHAYTLNDDVDEATKQRRLDELMQVQQNISDSINQKKVGNVFKTLFDRKEG
                     NFFIGRTEYDSPEVDNEVLVDANENYVRVGDFANIKITSAEAFDLYGTLC"
     gene            523..1023
                     /locus_tag="PK134_04450"
     CDS             523..1023
                     /locus_tag="PK134_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRB38130.1"
                     /translation="MFAFLFGPASTATSIYEFKVDGLSGGQIDFSKYKGKKILIVNTA
                     SKCGYTPQYSDLEKLYEKYKDKLVIVGFPANNFLWQEPGSNNEIAEFCKKNYDVTFPM
                     AAKVSVKGRDMHPLFRWLIEQPNVDFTGGIKWNFEKFLIDENGKLIHRFRSSVLPTSP
                     EITKYL"
     gene            complement(1029..2921)
                     /locus_tag="PK134_04455"
     CDS             complement(1029..2921)
                     /locus_tag="PK134_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRB38131.1"
                     /translation="MVFGAVMLLRESIVRNKVNSFCTSFHNKTGGNINIGKIQFNGLR
                     VIEIENISLVSPLNDTLLHTDTFMVQLSLLKLIRLKNPVSNFSAHGIKIKIASGDSIS
                     NYSFLFRKSKPENTEITSVQSTQASKVFTLWNKLTELSDLSFDLERCKITIIRNKETR
                     NFYFPEIINRRGKCRFIAYSNDHLQTEKWQAGFNIDDDKNEMSLNIKKLAGENWLPFF
                     DVDNKPKIAYSAISLQIKQPSRKDTTFQVKAELDSLSVNYWRIANDDVDFNKLSFSGI
                     CSFNNQRIETDSAVNITINKIHFKTKAAFDRKQPHSYALSLAFDESADDFFESLPKGI
                     FYHFKNFKAKGRLKFDLNFALDMSAPDSLIFNSDLTGNALTIEKYGDENFSRINNTFL
                     FDAYDGDRLQRSFLIGPENSDFTPLNEISHYLQYAVLTSEDPSFFYHRGFIPDAFKES
                     MIENIKAKRFVRGGSTISMQLVKNVFLSREKTISRKLQEMLIVWLIENKGLVSKERMF
                     EIYLNAIEWGPGIYGIKEASQFYFNKLPSQLNLAESIFLASIIPRPKYYRYSFDAQGN
                     LKSYLASYYRTVSNRMLSKTWISPIDTFNLQPQVKLKGPALKIVMPADSSMTDSLQWN
                     QSNELF"
     gene            complement(3027..3761)
                     /locus_tag="PK134_04460"
     CDS             complement(3027..3761)
                     /locus_tag="PK134_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HRB38132.1"
                     /translation="MRVKISFTRDNTSGNSIPLHHQKLIYDSLKTVVHEVGGNMKQLN
                     FSSLKGTSKIQNGFMKFLSSKITLVISGSNDDLITKVSNRIFEKNFMPIGRMNLLPKG
                     KEITSDPEFTTQMRYLCISPLVLFDPIKDAARSTEQIDPTSHNFSDILFNTTLDAMEK
                     SGVPEATISSYAEFDVQPDAEYMQKAAAANKKFARVYRSAEGKNMIGYLVPFTLHAHP
                     DVHRYVWHNGIGVMTHEGYGMLDLAK"
     gene            complement(3873..4964)
                     /locus_tag="PK134_04465"
     CDS             complement(3873..4964)
                     /locus_tag="PK134_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRB38133.1"
                     /translation="MLKSALNLLLWLITIFIAVVTFMAVAAAYISPAKIWFLALFGLI
                     FIYIYLVAFIWMLVLSFRHKKMMLLILIPLIAGLPTFVAHWNYSSSNQKATGENTIKV
                     LSFNVRVFDLYNWTHNLQTRSNIFNFLKKSDAGIYCFQEYYTSKLMPFNNTDSLKSLL
                     GISNVHTMLPIIERDSDEWGIATFSKYPILNKETVFTDKESANGCIATDLLIGNDTVR
                     VYNIHLQSVRLAKNDYIFVSEFNNKDNTQKMKGSKQIVQRLKKAFIKRAHQVCKVTTH
                     IGACPYKVIVAGDFNDTPASYAYNQISNGLTDSFLSCGKGLSSTYAGMFPGLRIDYIL
                     HSKSIKTIEYIRPLVHLSDHYPVISTLNF"
     gene            complement(4964..5848)
                     /locus_tag="PK134_04470"
     CDS             complement(4964..5848)
                     /locus_tag="PK134_04470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF020140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRB38134.1"
                     /translation="MLHNVKSNYKSLDMLQKIIVVNAIIYLFVNIGDVVLRLFNFPDG
                     TVSGFLTDWLAVPSSLKSLLYRPWTLITYCFYHENFLHILFNMLWLFFMGRLFTEYLG
                     EKKLLPLYLVGGLCGSVLFIVFYNLFPLFSREVDIAVALGASASVLAVTVAIATLLPD
                     YEVGLFLPQWRVKLKYIAIALFVIDLLSITGNNAGGNIAHIGGAIFGFIYIKQLKKGR
                     DLTGWLQRMIDRITQKKKPSIKVVYRKDSSDESYHNKKANNQEAIDKILDKISRSGYA
                     SLTNEEKEILFKASKNQD"
     gene            complement(5851..>6263)
                     /locus_tag="PK134_04475"
     CDS             complement(5851..>6263)
                     /locus_tag="PK134_04475"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRB38135.1"
                     /translation="HIPYQYLNADYAMAVENIKSNWYAHGDSPMIIEQARQAIDEYIR
                     FKTNIPTVGASGAVYGVLMAFGILFPNTLIYLYFFIPLKAKYLVLIIGGIELYSAWQS
                     NPTDNVAHFAHLGGMLFGYLLLRYWQKNNRSQLF"
BASE COUNT         2011 a         1059 c         1156 g         2037 t
ORIGIN      
        1 gtgcgtaata aaattccgga tattgccatt cgtacaacat ttatttcggg ttatccgggt
       61 gagacagagc aggatcatga aacattaatt cgttttcttg aagaaatgcg atttgagaga
      121 gtaggtgttt ttacttattc acacgaagag aatacacatg cttacacatt gaatgatgat
      181 gttgatgaag ccactaagca acgcagattg gacgagttga tgcaggtgca acagaatatt
      241 tctgacagta tcaatcaaaa gaaagtcgga aatgtattca agacactttt cgacaggaag
      301 gaagggaact tttttattgg cagaacagaa tacgattcac cggaggttga taatgaagtt
      361 ttagtggatg ctaatgaaaa ttatgttcgt gttggtgatt ttgccaatat aaaaatcact
      421 tctgccgagg catttgattt atatggaaca ctttgttaaa ctttttttac aagaggttgt
      481 tttaaatctg ttaattacaa aacaatgaaa ctgttgctga ttatgttcgc atttcttttt
      541 ggtcctgctt caactgctac ttcaatttat gagtttaaag ttgatggtct ttcaggaggg
      601 cagattgact tctcaaaata taaagggaag aaaattctga ttgtaaatac agcatctaaa
      661 tgtggttata caccgcaata cagtgatttg gaaaaactgt atgagaaata taaggataaa
      721 ttggttattg ttggatttcc tgcaaataat tttctgtggc aggagcccgg aagcaacaat
      781 gaaattgctg agttctgtaa gaaaaattat gatgtaactt ttccaatggc agcaaaagta
      841 tcggttaagg gcagagatat gcatccgctt ttccgttggc ttatagaaca acctaatgta
      901 gattttacag gaggcataaa atggaacttc gaaaaattct taattgatga aaacggaaaa
      961 ctgattcatc gttttcgctc aagtgtgttg cccacaagtc ctgaaataac taagtacctt
     1021 tagtctcttt aaaataattc attcgactga ttccattgca gtgaatctgt cattgaacta
     1081 tcagcaggca ttacaatttt taatgccggc cctttaagtt tcacctgcgg ttgaagattg
     1141 aatgtatcta ttggtgaaat ccatgttttg gaaagcattc tgttgctcac cgttctatag
     1201 taggaagcaa ggtaagactt caagttacct tgtgcatcaa atgaataacg atagtatttt
     1261 ggtcggggga ttattgatgc aagaaaaata ctttctgcca aattcagttg tgaaggaagt
     1321 ttattgaaat agaattgtga tgcttctttt attccataaa ttccgggacc ccattcaatg
     1381 gcattaaggt aaatttcaaa cattctttct ttactcacca atcctttgtt ttctattagc
     1441 caaacaataa gcatctcctg tagcttacgc gaaattgttt tttcacgaga caaaaacaca
     1501 ttttttacca attgcatgct gatggtgctt ccaccacgca caaacctttt tgctttaatg
     1561 ttttcaatca tgctttcttt aaaggcatct ggaataaaac cacgatggta gaaaaaagaa
     1621 ggatcttctg aagtgaggac ggcatattgc agatagtgac tgatctcatt cagtggagta
     1681 aaatcactgt tctcaggccc aatcagaaaa cttcgttgca atctgtcacc atcataggca
     1741 tcaaacagaa atgtgttgtt tatacgtgag aaattctcat caccatattt ttcaattgtc
     1801 aatgcattgc ctgttaagtc tgaattaaat atcagagaat cgggggctga catgtcaagt
     1861 gcaaagttta aatcaaattt taatctgcct ttagctttga agtttttgaa gtgataaaaa
     1921 attcctttgg gaagcgattc aaaaaaatca tctgcacttt cgtcaaatgc taaagaaagt
     1981 gcatagctat gaggctgttt tctgtcgaag gcagcttttg ttttaaaatg gattttattg
     2041 atggtaatgt ttacagcgct atcggtttca attcgttgat tattgaatga gcaaattccg
     2101 gaaaacgaaa gtttattgaa atcaacatca tcgttggcaa tacgccaata atttacactc
     2161 aggctatcaa gttctgcctt aacctgaaat gttgtatcct ttcttgaggg ttgtttaatt
     2221 tgtagtgaaa tagccgaata agcaatcttt ggtttgttgt caacatcaaa gaatggaagc
     2281 cagttttctc ctgccagttt tttgatgttt agtgacattt cgtttttatc atcatcaata
     2341 ttaaagccgg cttgccattt ttctgtttgt aaatggtcgt tgctgtaagc tataaagcgg
     2401 catttccctc tgcgatttat aatttccgga aagtaaaagt ttctggtttc tttatttctg
     2461 atgatcgtaa ttttgcatcg ttccaaatca aaacttaagt cggacaactc tgtcaactta
     2521 ttccaaagtg taaatacttt tgaagcctgt gtactttgta ctgatgttat ttctgtgttt
     2581 tcaggttttg attttctaaa aagaaaggag tagttactaa ttgaatcacc ggaggcaatt
     2641 ttaattttaa tgccatgtgc cgaaaaattg gagacaggat tttttagacg aataagcttt
     2701 aataatgaaa gctgcaccat aaaagtatct gtatgcaaca gtgtgtcatt taatggtgaa
     2761 accaaactga tgttttcaat ttcgatgacc ctgagtccat tgaattgaat ttttccgatg
     2821 ttgatgttgc caccggtttt attgtggaat gaagtacaaa aagaatttac tttattgcgt
     2881 acaatagatt cccgaagtag cattaccgcg ccaaatacca caacgaaaac tgtaagaata
     2941 attatgaaat atttttttat caaagcaaca tgaaatcagc aatattattc ctgactaaaa
     3001 ttataaataa aaacgatttc agtaaattat tttgccaaat caagcatgcc atagccttca
     3061 tgggtcatca ctccaatgcc attatgccaa acataacggt gaacatcggg atgtgcatgc
     3121 agtgtaaaag gaactaaata tccaatcata ttcttgcctt cagcacttct gtaaacacgg
     3181 gcaaattttt tatttgctgc tgcagctttt tgcatatact ctgcatcagg ctgtacgtca
     3241 aattctgcat aagagctaat agttgcttca ggcacacctg acttttccat ggcatcaagt
     3301 gttgtgttaa ataatatgtc ggaaaagtta tgtgatgtag gatcaatctg ctcagtgctg
     3361 cgtgctgcat ctttaatagg atcaaaaagt actaaaggag atatacagag atagcgcatc
     3421 tgcgtggtaa actcagggtc agaagtaatt tcttttcctt ttggaagcag attcattctg
     3481 ccaatgggca taaaattttt ctcaaatatt ctgttgctga cttttgttat taaatcatca
     3541 ttactgcccg atatcactaa agtaatttta gaagataaaa acttcataaa tccattttga
     3601 atttttgagg ttccttttaa tgacgaaaaa tttagctgtt tcatattgcc accaacttcg
     3661 tgaacaacag tttttaaact atcgtaaatt agtttttgat gatgcaatgg aatactattg
     3721 ccggaagtat tgtcgcgggt gaatgaaatt tttactcgca taattgaaaa atgttaatca
     3781 gtttagttag tgaaaatagt ttggttatgt caatgaaaat tagaattgtt ccaaaagtaa
     3841 aaaaataatt cacaaattca taaataaaaa tgttaaaaat ttagggtaga aataacaggg
     3901 tagtggtctg ataaatgcac taatggtcga atatattcta tggttttaat gcttttactg
     3961 tgtaaaatat agtcaattct aagtcctgga aacatacctg cataagttga ggacagtcct
     4021 tttccacagc taagaaaact atctgtcaag ccattgctga tttggttgta ggcgtaagat
     4081 gcaggtgtgt cgttaaaatc accggcaaca attactttat acgggcaagc acctatatga
     4141 gtggtaacct tgcaaacctg atgcgctctt tttataaacg cctttttcaa acgttgaaca
     4201 atttgcttag agcctttcat tttctgagtg ttgtctttat tgttaaactc actcacaaaa
     4261 atatagtcgt tttttgccaa tcgtactgat tgcaggtgta tgttataaac tctcactgta
     4321 tcgtttccaa tcagcaaatc ggtagcaatg catccgttgg cagattcttt atctgtaaat
     4381 actgtttctt tattcagtat tggatatttg ctgaatgtag caatgcccca ttcatcagaa
     4441 tcgcgttcta tgattggcag catcgtgtgt acattggata ttcctaacaa tgattttaat
     4501 gaatcggtgt tattaaatgg cattaactta ctcgtataat attcctgaaa acagtaaata
     4561 cctgcatcac tttttttcag aaagttaaaa atattacttc ttgtttgcag attatgcgtc
     4621 cagttgtaaa gatcaaaaac acgcacattg aatgaaagaa cttttatcgt attttctcct
     4681 gttgcttttt ggtttgatga agagtaattc caatgtgcaa caaatgttgg taagcctgct
     4741 attaatggaa ttaaaatcag cagcatcatt tttttatgtc tgaatgataa gaccaacatc
     4801 cagataaatg ctaccaaata gatataaata aatatcagac cgaatagtgc aagaaaccaa
     4861 atttttgcag gactaatata tgccgctgca acagccataa aagttacaac agcaatgaaa
     4921 atggttatta gccatagcaa caagttaaga gcagacttga gcatcaatcc tgatttttac
     4981 ttgctttaaa aagtatttct ttttcttcgt tggtgaggct tgcatatcct gaacggctta
     5041 ttttatccag aattttatca attgcttcct gattgtttgc ttttttgttg tgatacgatt
     5101 catcagaaga atctttgcga taaacaacct taattgatgg ttttttcttt tgagtaattc
     5161 tatcaatcat tcgttgaagc catccggtta aatcacgtcc tttttttaat tgcttgatat
     5221 agataaaacc aaagatggca ccacctatgt gagcaatgtt tccgcctgca ttgttgcctg
     5281 taatacttaa caggtcaatt acaaaaagtg caatggcgat atatttcagt tttacccgcc
     5341 actgtggcag aaacagacct acttcgtaat ccggtaacag tgttgcaatg gcaacagtaa
     5401 cagcaagcac acttgcagag gcacctaagg caactgcaat atcaacttcg cgactgaata
     5461 gtggaaacag attgtaaaat acaataaaca acacagagcc acataagcca ccaacaagat
     5521 aaagaggtaa taattttttt tcaccaaggt attctgtaaa aagacgaccc ataaaaaaca
     5581 gccataacat attaaacaga atatgaagaa agttttcgtg gtagaaacaa taggtgatta
     5641 atgtccatgg acgatacagc aatgacttga gagaagaagg aacagcaagc cagtcggtta
     5701 aaaagccgct tactgtgcca tcaggaaaat taaaaagtct aagcacaaca tcgcctatgt
     5761 ttacaaacaa ataaataatt gcattgacta caattatctt ttgcagcata tcgagcgact
     5821 tgtaattact tttcacattg tgaagcattt tcagaaaagt tgtgaccggt tatttttctg
     5881 ccaatagcga agtagtaaat atccgaaaag catgccacca agatgtgcaa aatgtgcaac
     5941 attatctgtg gggttgcttt gccatgcaga atacaactct atgccaccaa taatgagtac
     6001 taaatatttt gcttttagag ggataaaaaa gtaaagataa attaaagtgt ttgggaaaag
     6061 aattccaaat gccatgagca caccataaac tgcacctgat gcaccaacag taggtatatt
     6121 ggttttaaaa cgaatatatt catcaatagc ctgacgcgcc tgttcaataa tcattggact
     6181 gtcgccatgt gcataccagt ttgatttaat gttttccact gccatggcat aatcggcatt
     6241 cagatattga tagggaatat ggt
//
LOCUS       DAORQT010000090         6230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2053_length_6230_cov_6.330850, whole genome shotgun sequence.
ACCESSION   DAORQT010000090 DAORQT010000000
VERSION     DAORQT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_6230_cov_6.330850"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..3278
                     /gene="secDF"
                     /locus_tag="PK134_04480"
     CDS             261..3278
                     /gene="secDF"
                     /locus_tag="PK134_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRB38136.1"
                     /translation="MQSKGAIKFLAILVSIICLFQLSFTIVTNRIESKAHEATNGNPE
                     AYKAYIDSISQLTAYNLGFKKYTYQECKEREINLGLDLKGGMNVTMEVSVTDLIRNMA
                     NNPNDPMLVKALDDAHQAQRTSQKGLVQLFGEAFERNNPGAKLAALYSTMDLQNRVNF
                     NSTNAEVIKVIQSEADAAISRTYNILRTRIDKFGVTQPNIQQLGGGRILIELPGVKEP
                     DRVRKLLQGTAQLEFWETYDNAEVFPQLEAVNKKLAALETPAPTDSTAKSDTTVAATG
                     DSLTAAADTGAMKKPALLSKLESDTAAAKDTSSSAQFAKFSKENPLFALLTPAIFTNA
                     QNQSEYRKGPVVGYAAIKDTGKLREILERPEIKNILPRDLRLLWTAKPPTKESKFLEL
                     VAIKVKSRDGKAPMDGSVITDARQDFGQFNSSPEISMSMNPEGAKTWKRLTGENVGKS
                     IAIVLDNYVYSFPNVQGEIAGGRSSITGNFEISEAQDLANILKAGKLPAPARIVEEAI
                     VGPSLGEEAIQNSWMSFAGAFVVILLFMGFYYMRAGWVANIALFANIFFIMGVLASLG
                     AVLTLPGIAGIVLTIGLSVDANILIFERVREELKAGKGIRLAVSDGFKHAMPSILDSN
                     ITLLILGIVLYIFGSGPVQGFATTLVIGVLTSLFSAVLLSRLIFEGMLDKNKPISFGN
                     EFSNNIYKNIHFNFVDNRKKYYILSSIVIIAGIVSFAIKGLNYGVDFEGGRTYTIQMD
                     KSISTEDIRMALEPQLVEAPEVKSVGTTDRFKITTAYLIHDTASNVENVIVDKLYKGL
                     TPLYGSAPDFETFKTKNIISSQKVGPTIADDIKQSAVWAVLISCALMFVYIFIRFKKW
                     QYGLGAVVALFHDVLIVLSFFTLLSGVIPVALEIDQHFIAAILTVMGYTMTESVVVFD
                     RIREYLGHHHTADDKHVINEALNATLSRTINTTLTVFFVLIVIFIFGGEVIRGFIFAL
                     LIGRIIGTYSSLCISTPIVIDLGGNAHEGVAKK"
     gene            complement(3572..4198)
                     /locus_tag="PK134_04485"
     CDS             complement(3572..4198)
                     /locus_tag="PK134_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38137.1"
                     /translation="MKKHKQLSNLSTFAFIIAFLLTSCSSIIGTNKLKQVDEKTILHY
                     AKKYNINSTENYELDTAYFTFLKSFDTKLFKQQIKNHYPPLQALYYNKTGQLQSFQIN
                     CYAGGFPNLKWNKNGIIKTFPPKQQAPLDTIISLKTQLKFLKPLSNTEKSTTNDYYYI
                     VIIYWSKFMGRQSKRLIGFVQDNVKLATDKRVKIIYVNTDNFFADNLK"
     gene            complement(4241..5470)
                     /locus_tag="PK134_04490"
     CDS             complement(4241..5470)
                     /locus_tag="PK134_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRB38138.1"
                     /translation="MNLIIKIASRYLIGKKSHNIVNLVSLVSIAGVFTGTMALIVVLS
                     VFNGFGNLVISLYDTFDPDIKITVIKGKTFNSDALNLEKIRAIDGIKYAAFTLEENCL
                     ARYGEKQDIVTLKGLDNELMKKTGLNKAVVIGLPILENGERNFAIVGSGVAYALSVNA
                     DTGNEPLMLYLPDKNITSPGDFENALRQMNISIGGIFAIQQDFDSKYILAPMSFVQEF
                     IAAEGKASAMEIMLKPGADETTVIQKLKEISGHDFTVKNRAQQHEFLYKILSSEKWAV
                     FLILSFIMVIGIFNILGTLTMLVIEKKKDIIILKNLGAGFTMVKKIFFMEGLLITFVG
                     VVGGLLAGGIICFLQQHFHLIKLGNADSFVVEAYPVQMQVTDFVSVFLVVFCIGALAS
                     YFIAAHLVKKQYAETGK"
     gene            5614..>6230
                     /locus_tag="PK134_04495"
     CDS             5614..>6230
                     /locus_tag="PK134_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB38139.1"
                     /translation="MKKLHMVDLISQYQKIKPEVDKAISDTVTSSAYINGPEVKAFQA
                     ELEKYLNVGHVINCANGTDALQIAMMALNLQPGDEVITASFTYVATAEVIALLKLKPV
                     LVEVIPGTFSIDPVAIEKAITPKTKAIVPVHLFGQCADMEPIMKIAAKHNLYVIEDVA
                     QAIGADYTFSDGRKQKAGTIGTIGCTSFFPSKNLGCMGDGGAIFT"
BASE COUNT         1981 a         1118 c         1171 g         1960 t
ORIGIN      
        1 gtacgggggg ataaagttca tgtaaatgaa taggagtaaa caataaaaaa gtaaaaagaa
       61 aagtattaca atgcatttaa aatgctctac atgtgctaat attctgaaac agagcactta
      121 attaattaac tattttgtgg tcagacctag acaaagaaat aggtagtgta ttgaaaaaca
      181 acactgtagg ttagtgctta ggtttgttaa aaacctttat atttgccgct cttaaaaaaa
      241 taccgaaaaa taaattattc atgcaaagta aaggcgcaat taaattttta gccattctgg
      301 tatccatcat ctgtctgttt cagttgtcgt ttaccatcgt aacaaatcgt attgaaagca
      361 aggcacatga ggcaactaat ggcaaccccg aagcatacaa agcatacatt gactctatca
      421 gtcagttaac ggcttataat cttggcttta agaaatacac ctatcaggaa tgtaaggaac
      481 gtgaaatcaa cctgggtctt gacctaaaag gtggtatgaa cgtaacgatg gaagttagcg
      541 ttaccgattt gattcgtaac atggccaaca atcctaacga tccaatgtta gtgaaagctt
      601 tagacgatgc acatcaggca caacgtacaa gccagaaagg tttagtacag ttgtttggtg
      661 aagcctttga acgtaacaat ccgggagcaa aactggctgc actttacagc accatggact
      721 tgcagaacag agtaaatttc aactctacca atgccgaagt aattaaggtt atacagtcag
      781 aagcagatgc agctatcagc cgcacataca acattcttcg tacacgtatt gataaattcg
      841 gtgtaacaca accaaacatt cagcaattag gcggaggacg tattttaatt gagcttcccg
      901 gagtaaaaga gccggatcgt gtgcgtaaac tgttgcaagg aactgcacaa ttagagttct
      961 gggaaacgta tgataatgct gaagtgtttc cgcaattaga agcagtaaat aaaaaacttg
     1021 ctgcacttga aacacctgca ccaacagact ctactgcaaa atcagacaca acagttgcag
     1081 ctaccggtga ttctttaact gctgctgcag atacaggagc aatgaaaaag ccggcattac
     1141 tcagcaagtt agagagtgat actgctgctg caaaagacac ttcttccagt gctcagtttg
     1201 caaaattttc taaagaaaat ccactctttg cattgctgac acctgcgatc tttaccaatg
     1261 cacaaaatca gagcgaatat cgcaaaggcc ctgtagtagg ttatgctgct attaaagata
     1321 caggtaagct gcgtgaaatt ttagagcgtc ctgaaataaa aaatattctt ccacgcgatt
     1381 tgcgtttgtt atggacagca aaaccaccaa caaaagaatc taaattttta gagttagtag
     1441 ctattaaagt aaaaagccgc gatggaaaag caccaatgga tggttctgta attacagatg
     1501 cacgtcagga ctttggtcag tttaacagct cgccagaaat ttctatgtca atgaatcctg
     1561 aaggagctaa aacatggaaa agacttacag gagaaaacgt aggaaaaagt attgctattg
     1621 ttctcgataa ttatgtttat tctttcccta atgtacaggg cgaaattgca ggaggccgtt
     1681 catccattac aggtaacttc gaaatttctg aagcacagga tttggctaac attcttaagg
     1741 caggtaaact tcctgctcct gcacgaattg ttgaagaagc tattgttggt ccttcattag
     1801 gtgaagaagc aattcaaaac agttggatgt cgtttgcagg tgcatttgtt gtaatcctgt
     1861 tgttcatggg attctattat atgcgtgcag gttgggtagc taacatcgca ttgtttgcca
     1921 acatattctt tatcatgggt gtgcttgcat ctttaggtgc tgtacttacg cttccgggta
     1981 ttgcaggtat cgtcctcacc ataggtcttt ctgtggatgc caacatcctt attttcgagc
     2041 gtgtgagaga agagttaaaa gcaggcaagg gaataagact tgctgtttct gatgggttca
     2101 agcatgccat gccttctatc cttgactcaa acattacttt gctcattctc ggtatcgtac
     2161 tttatatttt cggttccggt ccggtacagg gttttgcaac tacattggta attggagtac
     2221 tcacatcact gttttctgct gtattgcttt caagactcat ttttgaaggt atgttggata
     2281 agaacaaacc aatttctttc ggtaatgaat tctccaataa catttacaaa aacattcact
     2341 tcaactttgt tgacaacaga aagaagtatt acatcctgtc ttcaattgta attattgcag
     2401 gtattgtttc tttcgcaata aaagggttga attacggtgt tgactttgaa ggtggacgca
     2461 cctacaccat tcagatggat aagagtatct ctaccgaaga tattcgtatg gcattggagc
     2521 ctcagttggt tgaagcaccc gaagtaaaat cagtaggtac aacagatcgt ttcaaaatta
     2581 ctactgccta tcttattcac gacacggcaa gtaatgtaga aaatgttatc gttgataaac
     2641 tttataaagg actgacacca ctttatggtt ctgctcctga ttttgagact ttcaaaacaa
     2701 agaatatcat ttcatcgcaa aaagtaggac ctactattgc agatgatatt aaacaaagcg
     2761 cagtgtgggc tgtattgatt tcatgtgcgc tgatgttcgt ttacatcttt atccgattca
     2821 aaaaatggca gtatggactt ggtgccgtag ttgccttatt ccatgacgta ttaattgtat
     2881 tgtcattctt tacattactg tcaggagtta ttcccgtagc acttgaaata gatcagcact
     2941 ttattgcagc tatcttaaca gttatgggct ataccatgac agaatcggtt gtagtgttcg
     3001 accgtattcg tgaatatcta ggacatcatc atactgccga tgataaacat gtgattaacg
     3061 aagcattaaa tgccacatta agccgaacca tcaacactac attaactgta ttcttcgtgt
     3121 tgattgtaat ctttattttc ggtggcgagg taattcgtgg tttcattttt gcactcctca
     3181 taggacgtat catcggaacg tactcttcgc tctgtatttc aacaccaata gtaattgacc
     3241 ttggtggaaa tgcacatgaa ggtgtagcga agaaataatt tcttaaaata ttttatttgg
     3301 aaaaggctgt ctcaaaaagg cagccttttt tgtgttttta aatttcaatg acctttaaaa
     3361 actaaatttt attattggaa aattgagaat taactaagcc gttcattttc ttgttgtact
     3421 tgtaattcct cctgaaaaaa tttggtttat tacatctggc attttaagat atggcgacaa
     3481 ctttgtacaa aaactgaatt gtatttcaaa agtaaaatct tatgaataag ctttaataaa
     3541 acatattgca cacatgctgt catgttattt attattttaa attgtctgca aaaaaattgt
     3601 cggtgtttac ataaataatt tttactcttt tatctgtagc aagttttaca ttgtcctgaa
     3661 caaatcctat taatcgtttg ctttgtctgc ccatgaattt actccaataa ataataacaa
     3721 tatagtaata atcatttgtg gttgattttt ctgtatttga caggggtttt aaaaatttca
     3781 attgtgtttt taacgaaata atagtgtcta atggtgcctg ttgttttgga ggaaatgttt
     3841 taataattcc atttttattc cattttagat ttggaaatcc acctgcataa cagtttattt
     3901 gaaatgattg aagttgtccg gtcttgttgt agtaaagtgc ctgaagaggt gggtaatggt
     3961 ttttgatttg ttgtttgaaa agcttcgtgt caaatgactt tagaaaagta aaatatgcag
     4021 tatctaattc gtaattttct gttgaattaa tattgtactt ttttgcataa tgtagaattg
     4081 ttttttcgtc aacttgtttg agtttattcg ttccaattat tgatgaacaa cttgtcaaca
     4141 agaaagcaat aataaaagca aaggttgaca gatttgatag ttgcttatgt tttttcatta
     4201 gtatgttcta tcgttgttta agtcatctaa aaattgtcaa ttacttccct gtttctgcat
     4261 attgcttttt tactaaatgt gctgcaataa aataggaagc aagagcaccg atacaaaaaa
     4321 ctactaaaaa cacacttaca aaatcggtta cctgcatttg aaccggatag gcttctacaa
     4381 caaaagaatc ggcattgcct aatttgataa gatgaaaatg ttgttgcaga aaacaaatga
     4441 tgccacctgc caacaagcca ccaacaacac ctacaaaagt gattaaaagt ccctccataa
     4501 aaaatatttt tttaaccatt gtaaaaccgg cacccaagtt tttaagaatg ataatgtctt
     4561 tctttttctc tataacaagc atagttagtg tgcccagaat attaaagatg ccaattacca
     4621 tgatgaaaga cagaatgaga aacacagccc atttctcaga actaagaatt ttataaagaa
     4681 actcatgttg ttgtgcacgg tttttaacag taaagtcatg accggaaatt tcttttaatt
     4741 tttgaatcac agtagtttca tcagcaccgg gtttcagcat aatttccatt gccgatgctt
     4801 ttccttcggc cgcaataaat tcctgaacaa aggacatggg tgccagaata tatttagagt
     4861 caaaatcctg ctgaatggca aatattccac caattgaaat gttcatttgt cgcaatgcat
     4921 tttcaaaatc accgggagaa gtaatgtttt tatcaggaag ataaagcatt agaggctcgt
     4981 tccctgtatc tgcatttacc gacagtgcat aagcaactcc actaccaaca atggcaaagt
     5041 ttcgttcacc attttccaga atcggtaaac cgataaccac agctttgttc aaccctgttt
     5101 ttttcatcag ttcgttgtca aggcctttca acgtaactat atcctgtttt tcaccatagc
     5161 gggcaaggca gttttcttct aatgtaaatg ctgcatattt tattccgtca atagcacgga
     5221 ttttttccag attaagagca tcactgttaa aagtttttcc ttttatgact gtaattttaa
     5281 tgtcagggtc gaaggtgtcg taaagtgaaa ttaccagatt tccaaagccg ttaaaaacac
     5341 tcagcactac aattaatgcc atagtgcctg taaacacacc ggcaatagac acaaggctga
     5401 ctaagttgac aatgttatgc gactttttgc cgatgagata acgagatgct attttgataa
     5461 tcaaattcat gcctgcgggc taaagtcagc aagaataatc acttttacaa caacgactta
     5521 aaaaatgccc ctactttttt acaaccttag ctttctgtta ataggcaata atagagctaa
     5581 atagtatctt cgctgcctca aatttgtaat gtgatgaaaa aactccacat ggttgacctg
     5641 atcagtcaat atcaaaaaat aaaaccggaa gtagataaag ctatttcaga cactgtaaca
     5701 tcatcagcct atataaacgg cccggaggtt aaggcatttc aggcagagtt agaaaaatat
     5761 ttaaatgttg gtcatgtaat aaattgtgca aacggtacgg atgcattgca gatagccatg
     5821 atggctctta atcttcaacc gggtgatgaa gttattactg caagttttac ttatgtagct
     5881 actgcagagg ttattgcact tttaaaatta aaacctgttt tagtagaggt tattcccgga
     5941 acattttcca tagatcctgt tgccattgaa aaggccatca caccaaagac aaaagcaata
     6001 gttccggtac atttattcgg gcaatgtgcc gatatggaac ccattatgaa aattgctgct
     6061 aaacataatc tttatgttat tgaagatgtg gcacaagcca ttggtgcaga ctacactttt
     6121 tctgatggca gaaaacaaaa agcaggaact atcggaacca ttggttgtac ttcgtttttt
     6181 ccgagtaaga atttaggatg catgggcgat ggcggtgcta tatttaccaa
//
LOCUS       DAORQT010000091         6205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2066_length_6205_cov_6.913496, whole genome shotgun sequence.
ACCESSION   DAORQT010000091 DAORQT010000000
VERSION     DAORQT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2066_length_6205_cov_6.913496"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PK134_04500"
     CDS             complement(<1..840)
                     /locus_tag="PK134_04500"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRB38140.1"
                     /translation="MNLLHIRRRIKKEFMLTIKKLFFIGFTFITLHATAQDDLMKMLN
                     ESGEKEKKEEVTATFKSTRLINGHSVENNKQGVLQLMILHRFGQINSGGYEFFGLDNA
                     TMRLGFDYGVTDWLNVGIGRSTFEKMYDGLLKVKLLKQRKSGTPVTLTAVASMQLNSL
                     KFTDPQRKNYFSSRLTYSYQLLAASKLSNAFSVQLMPTLIHRNLVPTANEKNDVFALG
                     GGGRIKISKSIAFTAEYYYVFPDQISSTYKNSLAVGFDIETGGHVFQIHFTNSRSMVE
                     KGFI"
     gene            complement(809..1360)
                     /locus_tag="PK134_04505"
     CDS             complement(809..1360)
                     /locus_tag="PK134_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRB38141.1"
                     /translation="MKKFILCVLMTGMYYTQAQNVFTTNAGTISFFSEAPLENIDATS
                     KKGHCILNVTTGDIVSLVAIRSFKFEKALMEEHFNEKYLESDKYKDAVYKGKIQEPLI
                     TDRDTSYQVTINGNITIHGIEKPANYKANYSFKGGVPAIEGSFNVALKDHNVEIPKLV
                     IQNIAEVVKVTCKFELAPYQKKD"
     gene            complement(1494..1742)
                     /locus_tag="PK134_04510"
     CDS             complement(1494..1742)
                     /locus_tag="PK134_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38142.1"
                     /translation="MNKSLILISGIFIALGISSCYYDVEDELYPNTGNCDTLNITYSQ
                     TVAPIISSNCKVCHSASMAQGNVVIDTYAAIKTYVDNG"
     gene            complement(1749..2450)
                     /locus_tag="PK134_04515"
     CDS             complement(1749..2450)
                     /locus_tag="PK134_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38143.1"
                     /translation="MNRTVFFWLMLLTIATSSCKHEPQDLAIATNDSTATEDCSNDTI
                     YFKNIIQPIINASCAYSGCHDAATAADGVILDTYANIIRTGKVKAGKPEDSKLYKSLI
                     ENDPDDIMPPPPGSPLSSVQINDIYQWIMQGAKNNECLGCDSVNVTFSGSILPIMQTI
                     CQTCHSGIAPLGSLSLVSYDDVSAVAMTGQLVGAITHADGYFTMPDKEDSTKYIPNCK
                     IKQIIKWVNDGYPDN"
     gene            2607..2882
                     /locus_tag="PK134_04520"
     CDS             2607..2882
                     /locus_tag="PK134_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB38144.1"
                     /translation="MNGMLLFLDNLGGGELLVIMVFVLFFFGSKKVPDLARGLGRASR
                     EFKDAMNGVQREIEQSMQTPPPSQQPQKNVENPVTPEKNGGVDEKTD"
     gene            3001..5190
                     /locus_tag="PK134_04525"
     CDS             3001..5190
                     /locus_tag="PK134_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRB38145.1"
                     /translation="MSNVRFRALDATNGRIPVKITPPSVKVSDYFATNVFTKEAMFKF
                     LPKNAYNAVISAIETGEKIDRKIAGEIAHGMKEWAISKGATHYTHWFQPLTGATAEKH
                     DSFFDMSNDNSFEHFSGSSLVQQEPDASSFPSGGIRNTFEARGYTAWDPGSPAFIIES
                     PNGRTLCIPTIYVSYTGETLDYKAPLLKALHTLNEVAVPVAQYFDKDITRVSTTLGIE
                     QEYFLVDTSLFYARPDLAITGRTVFGHSPAKGQQLEDHYFGSIPDRVNAFMHDFEIEA
                     YKLGIPLKTRHNEVAPCQFECAPIFEEINLAVDHNQLLMDLMDKVARRHHFKVLFHEK
                     PYAGINGSGKHNNWSMVTNTGKNLLSPGGNPKSNLMFLTFFINTIKAVHEHADLLRAS
                     IASAANDHRLGANEAPPAIMSVFLGAQLSSILDNIEKKVGSSKMSVEDKTALKLNIGK
                     IPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSNSMAILNTIVADQLRKFRKEVD
                     ALIKKNVKKDEAILRVLRQYIVETKSIRFEGNGYSEEWVKEAKKRGLSNHQTTPVALD
                     AMVSKKSLKLFEDNHIFTHREAEARHEIMLETYIKKIEIESRVIGDLAQNHIIPAAFR
                     YQNFLAETVDNLKDCDLKSETYATQLQLIKEISAHVNAIKKNVDTMLASRDKGNKIAD
                     IRDKAIFYCDKVKVSFDTIREHVDALEMIIDDEIWPMVKYRELVTIR"
     gene            complement(5305..5880)
                     /gene="nadD"
                     /locus_tag="PK134_04530"
     CDS             complement(5305..5880)
                     /gene="nadD"
                     /locus_tag="PK134_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRB38146.1"
                     /translation="MKVGLFFGSFNPVHNGHMMIANYFAEFTDLEQVWLVVSPHNPLK
                     PAGSLLNDYQRLHLVETAIGSYKKIKISKIEFGLPKPSYTINTLVHLNEKFPQHEFVL
                     IMGSDNLITFHKWKNFEQILENYKIYIYPRPDSDGGELKNHPSVKIIQAPLIEISSTF
                     IRQAIKEGNDVRFYLPESVYNYIDEMNFYKK"
     gene            complement(5881..>6205)
                     /locus_tag="PK134_04535"
     CDS             complement(5881..>6205)
                     /locus_tag="PK134_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38147.1"
                     /translation="DGKKEGLWKYNNGEYIAEGEYADGLKFGTWHQTYKNGKTAFTGK
                     YIDGLEDGEHIYYNDNGKLHEVRQYKQGQRNGLWKIYDDKGVLILTINYQADKITRID
                     KIRIN"
BASE COUNT         1956 a         1131 c         1087 g         2031 t
ORIGIN      
        1 aataaatcct ttttctacca tactgcgtga gttggtaaaa tgaatttgaa aaacgtgacc
       61 tccggtttca atatcaaagc ctacagcaag tgagttttta tatgtgctgc taatctgatc
      121 aggaaataca taataatact ctgcagtaaa tgctatactc ttggaaattt ttattcttcc
      181 gccaccgcct aatgcaaata catcattttt ttcattagct gtgggtacta aattgcggtg
      241 aatgagtgtt ggcattagct gaacagaaaa cgcattagaa agtttagatg ctgccaacaa
      301 ctgataggaa taggttagcc tggaagaaaa ataattttta cgttgagggt ctgtaaattt
      361 taatgagttt agttgcatgg atgcaacggc agtaagtgta accggtgttc cacttttacg
      421 ttgcttcagt aatttaactt ttaataatcc atcatacatt ttttcaaatg tgctgcgccc
      481 tattccaacg tttaaccagt cggtaacacc atagtcgaaa cctaaacgca tggtagcatt
      541 atctaaacca aaaaactcat aacctccact atttatctgc ccgaaacgat gcaagatcat
      601 taactgcaat acaccttgct tattattttc aacactgtgc ccgttaatga gtctggttga
      661 tttaaatgtt gccgttactt cttctttctt ttctttctct ccactttcat tgagcatttt
      721 catcaggtcg tcctgagctg ttgcatgcag tgtgataaaa gtaaatccaa taaaaaacag
      781 ttttttaatc gttagcataa attctttttt aatccttctt ctgatatgga gcaagttcaa
      841 atttacaagt taccttaaca acttcggcaa tattctgaat aactaattta ggaatttcaa
      901 cattgtggtc ttttaaggca acattaaagc taccttcaat tgcaggaact cctcctttaa
      961 aactgtagtt tgccttataa tttgccggtt tctcaatacc atgaatagta atatttccat
     1021 ttattgttac ctgataggaa gtgtctctat ccgtaatcaa tggctcctga attttacctt
     1081 tatatacagc atctttgtat ttatcacttt caagatattt ttcattgaag tgttcttcca
     1141 tcaacgcttt ttcgaactta aaagaacgga tagctactaa cgaaacaata tcgcctgttg
     1201 taacattcaa aatgcaatgc ccttttttgc ttgtagcatc aatgttttcg agcggtgctt
     1261 ctgaaaagaa agaaattgta cctgcattgg ttgtaaacac attttgtgct tgtgtataat
     1321 acatgcctgt cattaataca cataaaataa attttttcat tttttaatat ctaattattg
     1381 ggtgctccct gattaatcca gattgaaact tttttaatgt tgcaatcact taatttattt
     1441 tgattttttg gcataggttc atatcccgaa tcatgattga gtgaccctaa tagttatcca
     1501 ttatcaacat aggtcttaat tgctgcataa gtatcaataa caacgttgcc ctgcgccata
     1561 gatgccgaat gacaaacttt acaattgctg cttataattg gtgccactgt ttgtgaatag
     1621 gtaatgttaa gtgtatcaca gttgcctgta tttggatata actcatcttc tacatcataa
     1681 taacaactac taatgccgag tgcaataaaa atacctgaaa taagaattaa acttttattc
     1741 atgctttgtt aattgtcggg gtaaccatcg ttaacccatt taatgatttg ttttatttta
     1801 cagttaggaa tatattttgt tgaatcttcc ttatcaggca tggtaaaata tccatctgca
     1861 tgagttatag cgccaaccaa ctgtcctgtc attgcaacag cagatacatc atcataagaa
     1921 accaatgaaa gacttcccaa tggtgcaatt ccactatggc aggtctgaca aatggtttgc
     1981 ataatgggta agatactacc tgaaaatgtt acattaacac tatcacaacc caaacattca
     2041 ttgtttttag caccttgcat tatccattgg taaatatcat ttatctgtac gctgctcaat
     2101 ggtgaacccg gtggaggtgg catgatatca tcaggatcat tttcaataag tgatttgtag
     2161 agtttgctgt cttcgggttt accggctttc acttttcctg ttctgataat gttggcataa
     2221 gtatctaaaa taactccatc tgctgcagtt gcagcatcat gacatccgct ataggcgcat
     2281 gaggcattta ttataggctg aatgatgttt ttaaaataaa tggtatcgtt gctgcaatct
     2341 tcagtggcag tgctgtcatt ggttgcaatg gctaagtcct gtggttcgtg cttacaggaa
     2401 gatgttgcaa ttgtcaatag catcaaccaa aaaaatactg ttctattcat gcatgaatta
     2461 atttagaaac aaataaaaaa gatatattct aattaaagtc tataaaaatg tattaatggg
     2521 aaaatttatc ggctgaaagg taataaacac aaatattcat tgtttcaaca tgggcatttg
     2581 agttactttt gctcaaattt taagatatga acggcatgct tttgttttta gataatcttg
     2641 gtggaggcga attgttggta atcatggtgt ttgtattatt cttcttcgga tcaaagaaag
     2701 ttcctgactt agccagaggc ttgggccgtg ccagccgcga gtttaaagat gctatgaatg
     2761 gtgtgcagcg cgaaattgag cagagtatgc aaactccgcc accatcacaa caaccacaaa
     2821 aaaatgttga aaatcctgtt acccccgaaa aaaatggggg ggtagatgaa aaaactgact
     2881 aatttttggt gaaacactct attttttagg ggggtgtttg aaaaaaaaca atatttttct
     2941 tgacattgcc ctttttttca atgtattttt gagccgtttt aaaaaaaatc caatcactat
     3001 atgagcaatg ttcgtttccg tgccctcgat gctactaatg ggcgcatacc tgtaaaaatt
     3061 acccctccgt cagtaaaggt atctgactat tttgccacca atgtattcac caaggaagct
     3121 atgtttaagt ttttacctaa gaatgcatac aatgctgtta tctctgcaat agaaacgggt
     3181 gagaaaatag accgtaaaat agctggtgaa attgcccatg gcatgaaaga atgggctatc
     3241 agcaaaggtg ctacacatta cacgcattgg tttcaaccac tcactggtgc tactgccgaa
     3301 aaacacgaca gctttttcga catgagtaat gataactctt tcgaacattt ttcaggaagt
     3361 tctttagtac aacaggagcc tgatgcaagc agttttccaa gtggaggaat aagaaatact
     3421 tttgaagcac gtggctacac tgcatgggat ccgggctctc cggcatttat tattgaaagc
     3481 ccaaacggac gcacactttg tattcctaca atttatgttt cttacacagg tgaaacactc
     3541 gactataaag cgccattgtt aaaagcattg catacactga acgaagtagc agttccggta
     3601 gcacaatatt ttgataaaga cattacacgt gttagtacaa cattaggtat tgaacaagaa
     3661 tattttttag ttgatacttc tttgttttat gcacgacccg atttggcaat tacaggaaga
     3721 acagttttcg gtcactcacc tgcaaaagga caacaattag aagatcatta tttcggttct
     3781 attcctgata gagtaaatgc ctttatgcac gattttgaga ttgaagcata taaacttggt
     3841 attccattaa agacccgtca taatgaagta gcaccatgtc agtttgaatg tgctcctatt
     3901 tttgaagaaa taaatcttgc tgttgatcac aatcaacttc ttatggattt gatggataag
     3961 gttgcccgca gacatcattt taaagtgtta tttcatgaaa aaccttatgc aggaataaac
     4021 ggtagcggta aacacaacaa ctggagtatg gttaccaaca ccggaaaaaa tttattgtcg
     4081 ccaggaggaa atccaaaatc caacctcatg tttttaactt tttttatcaa taccataaaa
     4141 gctgttcacg aacatgcaga cctgttgcgt gcaagcattg cttcggcagc taacgaccat
     4201 cgtttaggtg ctaatgaagc accaccggcc atcatgagtg ttttccttgg tgcacagtta
     4261 agcagcatct tagataacat tgagaaaaaa gttggctcca gcaaaatgag tgtcgaagat
     4321 aagacagcat taaaactcaa cataggtaaa atacctgaaa ttttacttga caatacagac
     4381 cgcaaccgca catctccctt tgcctttaca ggaaataaat ttgagtttcg tgcagtaggt
     4441 tcttctgcca attgcagcaa ttcaatggct attttaaata ctattgttgc cgatcaactt
     4501 cgcaaattca gaaaagaagt agatgcttta attaaaaaga atgtaaagaa agacgaagct
     4561 attttgcgtg tgctgcgtca gtatattgtt gaaacaaaaa gtattcgttt tgaaggcaat
     4621 ggctatagcg aagagtgggt aaaagaagct aaaaagagag gactttccaa tcatcagaca
     4681 acacctgttg cattagatgc aatggtttca aaaaaatctc tgaaactttt tgaagacaat
     4741 cacattttta cgcatcgtga agccgaagcg cgacatgaaa ttatgcttga aacatatatc
     4801 aagaaaatag aaattgaaag tcgtgtcatt ggtgaccttg cacaaaatca tattatccct
     4861 gcagcattcc gatatcagaa ttttttagca gagactgttg acaacctcaa agactgcgat
     4921 ctgaaatcag aaacttatgc tacacagctg cagctgataa aagaaatcag tgcacatgta
     4981 aatgccatta agaaaaatgt tgataccatg cttgcatcgc gcgataaagg aaataaaata
     5041 gcagatattc gcgataaagc aattttttat tgcgataagg ttaaagtttc ctttgacaca
     5101 atccgcgaac atgttgatgc attggaaatg attattgatg atgaaatatg gccaatggtt
     5161 aaataccgag aattggttac gattagataa ttgatttgaa caacaaaaaa agctgtctat
     5221 tatggcagct ttttttgttt tgaatattaa tcaaacacct atctgttttt caaaaacttt
     5281 taagctttta agctttcaaa ctcattactt cttataaaaa ttcatttcat caatgtaatt
     5341 atacaccgat tcaggaagat aaaaacgaac gtcatttcct tctttgatgg cttgtctgat
     5401 aaatgtagat gatatttcaa ttaatggtgc ctgaataatt tttacagaag gatgattttt
     5461 taattcacca ccatcagaat ccggtcgagg ataaatatat attttatagt tttctaatat
     5521 ctgctcgaaa tttttccatt tatgaaaagt gataaggtta tcactaccca ttatcagcac
     5581 aaactcatgt tgtggaaatt tttcattcag atgaactaat gtattaattg tataggatgg
     5641 tttgggtaat ccgaattcta ttttcgaaat ttttattttc ttatagctgc caattgctgt
     5701 ttcaactaag tgtaatcttt ggtaatcgtt aagtaatgat ccggcaggtt taagcgggtt
     5761 gtgtggagaa acaaccaacc acacctgttc taaatctgta aactcagcaa aataattggc
     5821 aatcatcatg tgtccgttat gcacaggatt aaatgaacca aaaaataaac caaccttcat
     5881 ttagttaatt cttattttgt caattcgggt aattttgtct gcctgataat tgatggttaa
     5941 aatcaataca cctttatcgt cataaatttt ccacaaacca ttccgctggc cttgtttata
     6001 ctgtcgcact tcatgcaact ttccattgtc gttgtaataa atatgttcgc catcttcaag
     6061 gccatcaata tattttcctg taaatgcagt tttaccgttc ttataggtct gatgccatgt
     6121 accaaatttt aatccatcgg catattcgcc ttctgcaata tattcgccat tattatattt
     6181 ccatagccct tcttttttgc cgtca
//
LOCUS       DAORQT010000092         6096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2115_length_6096_cov_16.894223, whole genome shotgun sequence.
ACCESSION   DAORQT010000092 DAORQT010000000
VERSION     DAORQT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_6096_cov_16.894223"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PK134_04540"
     CDS             <1..730
                     /locus_tag="PK134_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRB38148.1"
                     /translation="IEVFAHGALCMAVSGKCYLSLHSHNASANRGACIQNCRREYIVQ
                     DKENEVELEIDNEYIMSSKDLCTIDFIDKLIETGISILKIEGRGRAADYVYTTTKCYK
                     EAVECYHNGSYSATKIEHWKNELATVFNRGFWGGYFLGKTMGEWSNTGGSQATTKKIY
                     VGKGVKYFPKNKVGEFKLESGTLSVDNDIMITGSTTGIILTKVQELRMDDLQVTTVKK
                     GDVFSMPVAEKIKPSDKLYKIVND"
     gene            723..950
                     /locus_tag="PK134_04545"
     CDS             723..950
                     /locus_tag="PK134_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRB38149.1"
                     /translation="MIEIVQYRSKCIGCNACVEASATRWRMSRKDGKCTLVGGKNNKG
                     IYIAVVNDDEYEQALQAAKNCPVNIIKVKKR"
     gene            951..1373
                     /locus_tag="PK134_04550"
     CDS             951..1373
                     /locus_tag="PK134_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38150.1"
                     /translation="MDAAIENFISAQKNMTLCTSVNDIPECANCFYVFMQDGAHLIFK
                     SKKTTGHVSHALVNNKVAGTILPDISKIGNIKGIQFSGIFLEPQDVMLEQAKKIYYSK
                     FPLALGISGDLWLIKLTYIKMTDNTLGFGKKLIWENKN"
     gene            complement(1387..4251)
                     /gene="gcvP"
                     /locus_tag="PK134_04555"
     CDS             complement(1387..4251)
                     /gene="gcvP"
                     /locus_tag="PK134_04555"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRB38151.1"
                     /translation="MYYSFADRHIGPDDKQVKHMLATIGVKSVNDLIAKIIPELIRLQ
                     KPLNLPEAMSEFEYLNELKATASENQVFKNFIGMGYYNTIVPGVIKRNIFENPGWYTA
                     YTPYQAEIAQGRMEALLNFQTMVCDLTGMQIANASLLDEGTAAAEAMVMFYYTRSKEA
                     EKKNACKFFVSENCFPQTIDVLRTRSKPLDIELVVGDEQLFSPDDSFFGALLQYPDAI
                     GNVLDYKNIVNMCHRHEVRVCVAADILSLVLLTPPGEWGADCVVGSTQRFGVPMGYGG
                     PHAAYFATHDVYKRNLPGRIIGMSVDAEGNPALRMALQTREQHIRRDKATSNICTAQV
                     LLAVMASMYAVYHGAEGLKAIATTVHKRTTALAESLKKAGFKVVNNSWFDTLTVEVED
                     TESVRDMAEANLINFRYNGNTVGVSIDETTSDEDLKDILRVFAASIGKKSIAAATMNG
                     NAIPAALLRSSEYMTNAVFNTHHSETEMLRYIKRLENKDLALNHSMISLGSCTMKLNA
                     TSEMEPLSWPEFANMHPFAPIEQAQGYAHIISELENFLKEITGFAAVSLQPNSGAQGE
                     YAGLVVIQKYHSDRGQSHRNVVLIPQSAHGTNPASAVLAGLKVVVVKCDDDGNIDLSD
                     LRLKAEQHQENLSCLMVTYPSTHGVYEENIKQITAAIHANGGQVYMDGANMNAQVGLT
                     CPALIGADVCHLNLHKTFAIPHGGGGPGMGPIGVAEHLAPFLPSHTVVPTGGRKGVNA
                     VSAAPWGSALILLISYGYIKMLGKTGVQRATEIAMLNANYIKARLEKHYPVLYKGKNG
                     FCAHEMILDCRAFKAVANVDVTDIAKRLMDYGFHAPTVAFPVAGTLMVEPTESEPLEE
                     IDRFCDAMIAIRHEIMEVINGEYSKEDNMLVNAPHTALEATADEWKHSYSRQKAVFPA
                     PWVAGNKFWPSVKRVDNAYGDRNLICTCPDVSAYETAEAV"
     gene            4606..5292
                     /locus_tag="PK134_04560"
     CDS             4606..5292
                     /locus_tag="PK134_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38152.1"
                     /translation="MNQITDMTLRHFITSLIFLFSATTLSAQDTLLLLNGKTKPVKLF
                     NLNDPDWVRYVKFKESDKKKNEGSARVKRIDVYKVFSIKKADGTEQVLYKPDSTSDDP
                     DIPWVRDFIAGQQYGIMHKRERFNKHDNQWHRRLNFTEVGGIAAGGAGSLLTFYGIPV
                     PAIYAIAVGRHSPALPNDPAIAPEMKNSEGFISGYQKQRRNQRIRQGFISGMIGFAVG
                     IVTFTLIERN"
     gene            5293..>6096
                     /locus_tag="PK134_04565"
     CDS             5293..>6096
                     /locus_tag="PK134_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRB38153.1"
                     /translation="MFRKIAHKAVSYNLLIALAASAASHLTILRINETNNVVLIAIIF
                     FATLAIYNFPRLPWQKPLNTRTWICLISILFSAILIINYNYISINLFLTAILACLIYM
                     MPFNYKGKRIKGIRNIFLLKNIFLAASWALITVLLPMAALGHESPIIKEVFLLRFIYV
                     FGISLLFDIRDTAKDKTRNHLTLPVVLGTTVTRYIVIASAALFILIVSVLYRHEHFTL
                     PMAVAFITSALLLVVATITAPPAANFKFYQVGVDSLLIAQWLLVVVAVKF"
BASE COUNT         1886 a         1256 c         1099 g         1855 t
ORIGIN      
        1 aatagaagtg tttgcccatg gtgctttatg tatggccgtt tctggcaaat gttatctgag
       61 tctccactca cacaatgcct cagcaaaccg tggtgcatgt attcagaatt gcagacgtga
      121 atatattgtt caggataaag aaaacgaagt tgagcttgaa attgacaatg agtatatcat
      181 gtcgtcaaaa gatttgtgca ccattgattt tattgacaaa ctaattgaaa cgggcatcag
      241 tattttgaaa attgaaggca gaggtcgtgc tgccgactat gtttatacta caacaaaatg
      301 ttataaagaa gctgttgaat gttatcataa tggcagctat tctgcaacaa aaattgaaca
      361 ttggaaaaat gaactggcaa cagtctttaa ccgtggtttc tggggtggtt attttcttgg
      421 aaaaactatg ggtgaatgga gcaataccgg aggctcgcag gcaacgacta aaaaaattta
      481 tgtcggtaag ggtgtcaagt attttccaaa aaacaaagta ggcgaattta agctggaatc
      541 aggaacacta tccgttgaca atgacattat gattaccggt tcaacaacag gaattattct
      601 taccaaggtt caggaattgc gaatggatga tttacaggtt acaactgtaa aaaaaggtga
      661 tgtgttttca atgccagttg ctgagaaaat aaaaccatca gacaaacttt ataaaatcgt
      721 taatgattga aatagtacaa tacaggagta aatgtattgg atgcaatgcc tgtgttgaag
      781 cctcggcaac acgatggcgt atgtcacgaa aagacggaaa atgtactctt gtaggtggaa
      841 aaaataataa gggcatctac attgctgttg taaacgatga tgaatatgaa caagcactgc
      901 aggcagcaaa aaattgtccg gtaaatatta ttaaagtaaa aaagagataa atggatgcag
      961 caatagaaaa ttttatcagt gcgcaaaaaa atatgacatt atgcacttcg gttaacgaca
     1021 tacccgaatg tgcaaattgt ttttatgtat tcatgcagga cggtgctcat ctgatattta
     1081 aatccaaaaa aactaccggt catgtttctc atgcacttgt aaataataaa gtggcaggca
     1141 caatattacc agacataagt aaaattggaa acatcaaggg cattcagttt tcaggaattt
     1201 ttcttgaacc acaagatgtc atgctggagc aggcgaagaa aatttattac agtaaatttc
     1261 ctctggcatt aggaatatcg ggcgacttat ggctgataaa attaacctac atcaaaatga
     1321 ctgataacac gcttggcttt ggaaaaaagc tgatatggga aaacaagaac tgatataaaa
     1381 aatcaattaa actgcttctg cggtttcgta agccgaaaca tcagggcaag tgcatatcag
     1441 gtttctgtca ccataagcat tatcaactct tttcaccgat ggccagaatt tatttcctgc
     1501 aacccatggt gccggaaata ctgctttctg acgtgagtaa ctatgcttcc attcatctgc
     1561 tgttgcttca agtgcagtat gtggcgcatt cacaagcata ttatcttctt tgctgtactc
     1621 accattgatt acttccataa tttcatgtct gatagcaatc attgcatcac aaaaacgatc
     1681 tatctcttct aatggctcac tctctgtagg ttccaccata agcgttcctg caacaggaaa
     1741 tgctactgta ggtgcatgaa aaccataatc catcagtcgc ttggcaatat cagttacatc
     1801 aacattggca acagctttaa atgcacgaca atctaaaatc atttcatgag cacaaaaacc
     1861 atttttgcct ttatataaaa caggataatg tttttctaat cgtgctttaa tataattggc
     1921 attgagcatt gctatttctg ttgcacgttg cacacctgtc ttaccaagca ttttaatata
     1981 accatagctg attaacaaaa tcaatgcact gccccatggc gctgcagaca cggcatttac
     2041 acccttacgt ccacctgtcg ggacaacagt atgcgaaggt aaaaatggcg caagatgttc
     2101 agctacacca ataggtccca ttccggggcc acctccacca tgtggtatgg caaatgtttt
     2161 atgcaaattc aaatggcaaa catcagcacc tatcaaggca ggacatgtta aacctacctg
     2221 tgcattcatg tttgcaccat ccatataaac ctgaccaccg ttggcatgta ttgctgctgt
     2281 gatctgttta atattttcct catacactcc atgtgttgaa ggatatgtca ccatcaaaca
     2341 agataaattt tcctgatgct gttctgcttt aagtcttaaa tcgctcaggt caatgtttcc
     2401 atcatcatca catttaacaa caacaacttt taatccggca agtacagcac tggcaggatt
     2461 ggttccatgt gcagattgtg gtatcaatac cacattgcga tgactctgtc ctctgtcgct
     2521 atgatatttc tgaataacaa ctaaaccggc atattctcct tgtgcaccgc tgttaggttg
     2581 taatgacacc gctgcaaatc ctgttatctc tttcagaaaa ttttccagtt cagaaataat
     2641 atgtgcataa ccttgtgctt gttctatagg agcaaatgga tgcatgttgg caaactcagg
     2701 ccagcttagt ggttccatct cactggtggc atttagtttc atggtgcacg aacctaaaga
     2761 tatcatcgaa tgattcaatg caagatcttt attttcgaga cgtttgatgt agcgcaacat
     2821 ttctgtttcg ctgtgatgtg tattaaatac tgcattggtc atatattccg aactgcgtaa
     2881 tagcgcagca ggtatggcat taccattcat agttgcagca gcaatagact ttttaccaat
     2941 acttgcagca aacacacgca aaatatcttt caaatcttcg tcagaggttg tttcatctat
     3001 actaacaccc actgtatttc cattgtaacg gaagtttatc agattagctt cagccatatc
     3061 gcgaacactc tctgtatctt ctacttcaac agtcaatgta tcgaaccatg agttgttcac
     3121 tactttaaat ccggctttct ttaaactctc tgcaagtgca gtagtacgct tatgtactgt
     3181 agtagcaata gccttcaaac cttctgctcc atgatacact gcatacattg atgccatcac
     3241 tgctaacagc acctgcgcag tacaaatatt acttgttgcc ttatctcgtc tgatatgttg
     3301 ctcgcgtgtc tgcaatgcca tacgtaaagc aggattgcct tctgcatcta cactcatacc
     3361 gataattctt ccgggcaaat ttcttttata tacatcatgt gttgcaaaat atgcagcatg
     3421 cggaccgcca tatcccatag gtacaccaaa acgttgtgtt gatcctacca cacaatcggc
     3481 accccattca ccgggaggcg ttaacaatac tagacttaaa atatctgctg ctacacaaac
     3541 acgaacttca tgtcggtgac acatgttaac aatgttttta taatccaaca cattacctat
     3601 tgcatcagga tactgaagta atgcaccaaa gaaactgtca tcaggcgaaa acaattgctc
     3661 atcgccaaca accaattcaa tatccaatgg tttgctgcgt gtgcgcaaca catctatagt
     3721 ttgcggaaag cagttttcag aaacaaaaaa cttacatgca ttttttttct ctgcctcttt
     3781 agagcgtgta taataaaaca tcaccattgc ttctgcagca gcagtgcctt catccagcag
     3841 cgatgcattt gcaatctgca tacctgtcaa atcgcacacc atagtctgaa aattcagcaa
     3901 tgcttccatc ctgccctgcg caatctctgc ctgataagga gtatatgctg tgtaccaacc
     3961 cggattttca aaaatgtttc gttttattac acccggcaca atggtattgt aataccccat
     4021 gccaataaaa tttttaaaaa cctgattttc cgaagctgtt gctttcaatt catttaaata
     4081 ttcaaactcg ctcattgcct ccggaaggtt taatggtttt tgcaaacgaa tcagttcagg
     4141 aataatttta gcaataagat catttacgga ttttacgcca atggtggcaa gcatgtgttt
     4201 aacctgcttg tcatcagggc caatgtgcct gtcggcaaaa gaatagtaca tgtactctgg
     4261 attttgccgc aaaagtaaat aattagaacg ttaaaaaatg tatttctgaa agcattcaac
     4321 atgatttaca tcaagcattt accgcttttt agcacaaaaa ttattaacac atgttgatat
     4381 ttcaatattt ctaaaacttg ggcatgtcac cgcctgtggc gctgtcgggc tatacgctgc
     4441 aagtccgctc ctgcctgcgg cagcatgcgg gcttttcgct actatccctc atgcaaaaaa
     4501 ccatctccaa aataaaaact ttcctcctcc gattcgatta tctatataac accacattat
     4561 caaattttta acaaatgcta tatattacat acatttttta tagttttgaa tcaaataact
     4621 gatatgacct tacgtcactt tataacatcc cttatttttc ttttcagcgc cacaacatta
     4681 agcgcacaag acacactgtt gctccttaat ggcaaaacca aacctgtaaa actttttaac
     4741 ctcaacgacc ccgactgggt gagatatgta aaattcaagg agagtgataa aaagaaaaat
     4801 gaaggaagtg cgcgtgtaaa acgcattgat gtttacaaag tgttctccat caaaaaagct
     4861 gatggcacag agcaagttct ttataaaccg gacagcacat cagatgatcc tgatattcca
     4921 tgggtgcgcg attttattgc aggacaacaa tatggaataa tgcataaaag agagcgtttt
     4981 aacaaacacg ataaccaatg gcaccgcaga ctaaacttta ctgaagttgg tggaatagct
     5041 gcaggcggtg caggaagtct tttaactttt tatggtattc ctgtgcctgc aatttatgcc
     5101 attgctgtgg gacggcatag ccctgcatta cccaacgatc cggctattgc tcctgaaatg
     5161 aaaaacagcg aaggatttat ttcaggctat caaaaacaaa gacgtaatca gcgaatcaga
     5221 caaggattta tttccggcat gataggtttt gctgttggca ttgttacttt cactctaata
     5281 gaacggaact gattgttcag gaagattgct cataaagctg tttcctataa cctactcatt
     5341 gcccttgcag caagtgcagc aagtcattta actattctca gaataaatga aacaaacaat
     5401 gtagttctta tagcaattat attctttgct actctggcta tctataattt tcctcgtttg
     5461 ccatggcaaa aaccacttaa cacacggaca tggatttgtc ttatttcgat tctattctca
     5521 gcaattttaa taatcaatta taattatatt tccataaatc tttttttgac agctattctg
     5581 gcttgtctca tttatatgat gccttttaat tacaagggca aacgcatcaa aggaattcgc
     5641 aacatttttc ttcttaaaaa tatttttctt gcagcaagtt gggcattaat aactgtactc
     5701 ttacctatgg ctgcattagg gcatgaatca ccaataataa aagaagtttt tttactccgt
     5761 ttcatttatg tattcggtat ttctttattg tttgatattc gcgatactgc aaaagacaaa
     5821 acacgcaacc acctgacact acccgtggtt ttaggaacaa cagtaacccg atatattgtt
     5881 attgcatctg cagcattatt tattttaatt gtttctgtac tttatcgtca tgaacatttt
     5941 acattaccta tggcagttgc atttatcaca agtgcactgt tgttggtagt tgcgacaatt
     6001 actgcaccac ctgcagcaaa ctttaaattt tatcaggttg gagttgacag tttgcttatt
     6061 gcacaatggt tattggtggt agttgccgta aaattt
//
LOCUS       DAORQT010000093         6083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2124_length_6083_cov_7.278699, whole genome shotgun sequence.
ACCESSION   DAORQT010000093 DAORQT010000000
VERSION     DAORQT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_6083_cov_7.278699"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PK134_04570"
     CDS             complement(<1..1028)
                     /locus_tag="PK134_04570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB38154.1"
                     /translation="MSFSNLNLITPLLEVLNELKYSKPTPVQEQSIPIVLAGRDIFGC
                     AQTGTGKTAAFALPILQHISLKKHKPGNHIKALVLAPTRELALQIGQSFSEYGRKLPY
                     RNVVLFGGVSQVNQTNALKNGVDILIATPGRLLDLMNQGFIDLKHIEYFVLDEADRML
                     DMGFINDIKKIIVKLPQQKQTLFFSATAAPEIMKLANTLLKNPAKVNVTPISTPAEQV
                     KQLVYHVKKENKRSLLRHVLESNKINHALVFTRTKRGADRVVKDLNKNGIKAEAIHGD
                     KSQGARERALNGFKKRSIQILVATDIASRGIDVDRLTHVINFEIPEQAETYVHRIGRT
                     GRAGESGIA"
     gene            complement(1316..2404)
                     /locus_tag="PK134_04575"
     CDS             complement(1316..2404)
                     /locus_tag="PK134_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRB38155.1"
                     /translation="MNKNNYCVIMAGGIGSRFWPMSRNAFPKQFLDILGTGQTLLQQT
                     FNRFTKICPIENIYIVTNESYIDLVKKNLPQLPVENILGEPLRRNTAPCVSYAAFKIA
                     AKNPDANIVVAPSDHIITKEDAFIEAINKGLTFTAQNDCLLTLGITPSRPDTGYGYIQ
                     FVDEKHNEINKIFKVKTFTEKPNLEMARFFLQSGEFVWNAGIFLWNLKSILKALKEHL
                     PEMYHLFEEGNSIYNSKGEDAFIKTAYSQCTNISIDFGVMEKAKNVFVMSADFGWSDL
                     GTYGSLYEHITHDANKNAVVGKNVMLYDTKNCIVNMPHDKLVVLQGLSDYIVVEAENI
                     LLVCRKQDEQQIRQFVNDVKSAKGEKFV"
     gene            complement(2611..6072)
                     /locus_tag="PK134_04580"
     CDS             complement(2611..6072)
                     /locus_tag="PK134_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRB38156.1"
                     /translation="MAMAQTDYRLMFLGKAVLPAENLNSFIQEKVSENDVYNGYYYRI
                     IQFYTVPTQAEKAVMETAGIKLLEYLPKNAFNAAIPISFNKQLLSGFNVRSVTNQSSE
                     QKLSISMLGDYPKYAVPSAGMVDVRLQFQKNISIQQAQQIAATYGKILSSTEINNMVE
                     VRMQQQSVATISQQPWLFFIDLTSPPSVKDDLEGRSLHRSNVINNDLLMGRKYNGSGV
                     AAALADDGFVGPHIDFTGRITNYTTTVGPSHGDMTGGILAGAGNLNPAYPGMANGVQL
                     HVFDIGSYPQIIDAVANYNNLGTVVASTSYSQGCNEYTTDTQFGDQTIHDNGQLEFVF
                     SAGNNNGTNCGYGAGGNWGNITGGYKQGKNVIAVANLDALEVIDPSSSIGPASDGRVK
                     PDIAANGRDQMSTDENNTYQVGGGTSAACPGIAGICTQLIQAYKQINSATDAPTALIK
                     ACLLNGAEDIGNPGPDYKYGWGRVNALRAVTTIEDARYFTDSINQSDSLTYSINVPAG
                     TSQMRVMVYWHDVGGSPASSTYLVNDIDMQVTDPSNAVWNPWVLDPTPNAANLNAPAT
                     RGIDHLNNMEQVTLDNPAAGNYVVSLKGFAIPQGPQTYYVVYEFRNDDITVTYPMGGE
                     GFVPGHPEIIRWDALKGLGSFDVEYTTDNGTTWNNIATVNQNTLQYSWNVPNNIATTG
                     DARVRVTRGSVSGLSDTTFTIVGIPQNLNVIYSCPDSIKIGWAAVSNAAWYEVSMLGT
                     MYMDSVATTTNTDYIFTNLIASNSYWFSVRAVMANGNKGRRANAIKKQLGIFNCPNIP
                     PSVQFNANFNVGCTSKTFTFTDQSSNAPSSWNWTFNPNTVTFVGGTSATSQNPQVQFN
                     TPGTYDVTLDATNGIGTSSSTQTAMINILAPSLPPMTQDFQATVFPPANWSIDSSNAN
                     YTWLKSALITGSAGTSTYAARFNNYSYNSSGSEDGLISYEVSLSNAAAALLTFDVAYA
                     QYSSVYNDGLRVDISTDCGNTWVPSGYLKTGVALATTGTTTSNFAPNAANQWRNDSVS
                     LNAYLGQDVSIKFVNINDFGNSMYVDNVNLDVTTGLNQLDNNVGTVNISPNPSDGKFI
                     IYSKQTSHESYTLTVYDINGKVIAEDVFPANNKSHAVDISNFSKGVYFIKVEGEKSVQ
                     RLKVISR"
BASE COUNT         1819 a         1148 c         1127 g         1989 t
ORIGIN      
        1 gctatacccg attcaccggc acgacctgta cggccaatac gatgaacata agtttcggcc
       61 tgttcaggaa tttcaaagtt aataacatgg gtcagtctgt ccacatcaat accacgagaa
      121 gcaatatctg ttgctactaa aatttgtatt gatctttttt tgaaaccatt caaagcccgt
      181 tctcttgcac cttgagactt gtcgccatga atagcctctg cttttatacc gtttttattt
      241 aaatctttta ctaccctgtc tgcaccacgt tttgttcttg taaaaaccaa ggcatgatta
      301 attttattgg actctaatac atgacgtaac aaggagcgtt tattttcttt ttttacatga
      361 tatactaact gttttacctg ttcagccggt gttgaaattg gagtaacatt gaccttagcc
      421 ggatttttta ataatgtgtt ggctaatttc atgatttccg gtgccgcagt tgcagagaaa
      481 aacaaagttt gcttttgttg aggaagcttt acaattatct tttttatatc attgataaat
      541 cccatgtcta acattctgtc tgcttcatct aaaacaaaat actctatatg ttttaaatct
      601 atgaacccct gattcattaa atcgagcaaa cggccgggag ttgcaattaa tatatcaact
      661 ccatttttta aggcatttgt ttggtttact tgagatactc ctccaaaaag tacaacattt
      721 ctgtaaggga gtttacgacc atattcggaa aaactttgac cgatttgtaa ggcaagttca
      781 cgagttggtg ctagcaccaa agccttgatg tgattgcctg gcttatgctt tttaagggaa
      841 atatgttgaa gaataggtaa ggcaaaggct gctgtttttc ctgttcctgt ttgggcgcag
      901 ccaaaaatgt cgcgaccggc taatactatt gggatggatt gttcctgaac aggagttggc
      961 ttactatatt tcaactcgtt taagacttcc aataggggag taatgagatt taaatttgaa
     1021 aatgacatta aataatatgg gatttttatc tgacggattt aagggttcat gtagagttat
     1081 ttaccctctt cttaaagaga agttaatccg tagttaactt gatttgctta ctataatata
     1141 actgttttgt aaatagtttg caaagtaaca acaaatttct taaaaaagct aagaatgatt
     1201 aaatttagtt tgagaatcgc atcatttatc gagtaaagtt aggcataaat gctaaaaatt
     1261 aacctaaatt gatggtgacg gattgatgtt cgcttaaact aaatgagata ttatatcaaa
     1321 caaacttttc gcctttagca ctctttacat cattcacaaa ctgacgtatt tgttgttcgt
     1381 cctgctttct gcaaaccaat aaaatatttt ctgcttcaac aacaatgtaa tcggacaagc
     1441 cttgtagaac aaccagttta tcatgtggca tatttacaat gcagttttta gtatcgtaca
     1501 acatcacatt tttcccaacc actgcatttt tattagcatc gtgtgttatg tgctcataaa
     1561 gtgaaccata agtacctaaa tcactccatc caaaatcagc actcatcaca aaaacatttt
     1621 tcgctttctc cattacacca aagtcaatgc tgatgttggt acattgcgaa taggctgttt
     1681 taataaatgc atcttcacct ttgctgttgt aaatggaatt accttcttca aaaagatgat
     1741 acatctcggg cagatgttct tttaaggctt ttaatattga cttaaggttc cataaaaata
     1801 ttcctgcatt ccagacaaac tcacccgatt gcagaaaaaa gcgagccatt tccagatttg
     1861 gcttttcagt aaaagtttta accttgaata ttttatttat ttcattgtgt ttttcatcta
     1921 caaactgaat gtagccatag cctgtatccg gtctgcttgg tgtaataccc aaggtaagca
     1981 ggcagtcatt ctgtgcagtg aaagtaagac ctttgttaat ggcctcaata aaagcatctt
     2041 cctttgtgat gatatggtct gacggggcga caactatatt ggcatcagga ttttttgcag
     2101 caattttaaa tgcagcatac gaaacacatg gtgctgtatt tcgtctgagt ggctcgccca
     2161 gaatattttc taccggtagt tgtggcagat ttttctttac taaatcaata tacgattcat
     2221 tggttacaat atagatattt tctataggac aaattttagt aaaacgatta aacgtttgtt
     2281 gtaaaagtgt ttgtcctgtt cctaaaatgt caagaaattg tttcggaaaa gcatttctgc
     2341 tcattggcca aaaacggctt cctatgcctc cggccattat tacgcagtaa ttgtttttgt
     2401 tcattatttt taaaagattg tcttaaattt tatttgatag attttaaacg caaaaaaatg
     2461 aaattaaagt ctggcagaat gaataatcgc ttttttttat taacgaatag atttgataat
     2521 tatttgtgtt ttggcttttc agtacaatat agtgttaaat aaaaaaccgg ctgatagtat
     2581 caaccggttt tataaatcat tgagttatcc ttatctggaa ataactttca aacgctgaac
     2641 agatttttct ccttcaactt ttataaaata aacacctttg gaaaaattgc tgatatcaac
     2701 agcatgactt ttgttgttag ccgggaatac atcttcagct ataacttttc cgttaatgtc
     2761 ataaacagta agtgtatatg actcatgtga tgtttgctta ctgtaaataa taaatttccc
     2821 gtctgatgga ttcggagaaa tatttaccgt tccaacatta ttgtccaatt gatttaatcc
     2881 tgtagtaaca tcaagattta cattgtcaac gtacatagag ttgccaaaat cgttgatgtt
     2941 tacaaatttt atcgaaacat cctgaccaag gtatgcattc aatgaaacag aatcattgcg
     3001 ccattgattg gcagcatttg gagcaaagtt gctggttgtg gtacccgtag tagcaagagc
     3061 cactccggtt tttaagtaac ctgaaggaac ccatgtattt ccgcagtctg ttgaaatgtc
     3121 aactcgcaaa ccatcattat aaacacttga atattgtgca tacgctacat caaatgtcag
     3181 taatgctgct gctgcattgg ataaacttac ttcataacta attaaaccat cttctgatcc
     3241 tgaactatta tatgagtaat tgttgaatcg tgcagcataa gtagaagtgc ctgctgatcc
     3301 tgtaatgaga gctgatttta accatgtata attagcgttt gatgaatcaa tagaccagtt
     3361 tgccggaggg aaaactgtag cctgaaaatc ttgtgtcatt ggtggtaaag aaggagcaag
     3421 aatattaatc attgcagttt gtgttgaact tgaagtgcca attccattgg tagcatctaa
     3481 agttacatcg taagtgccgg gtgtattaaa ctgtacttgt ggattttgtg atgttgcaga
     3541 agttccacct acaaatgtaa cggtattagg attaaatgtc caattccaac ttgacggtgc
     3601 attagagctt tgatctgtaa aagtgaaagt tttgcttgta caacctacat tgaagtttgc
     3661 attaaactgc acagatggag gaatgtttgg acaattgaaa atgccaagtt gttttttaat
     3721 ggcattagcc ctgcgccctt tattgccgtt agccatcact gcccttacgc taaaccaata
     3781 agagtttgat gctataaggt ttgtaaaaat ataatcggtg ttggttgttg tggcaacaga
     3841 gtccatatac attgtaccta acatactcac ttcataccaa gcagcattac ttacggctgc
     3901 ccatccaatt ttaattgaat caggacatga gtaaataaca tttaaatttt gtggaatgcc
     3961 aacaatggta aatgttgtgt cagacaatcc agaaacactt cctcttgtaa cacgaacacg
     4021 ggcatcacct gttgttgcaa tattattagg cacattccac gaatattgta atgtattctg
     4081 atttacagtg gcaatattat tccatgtagt tccattgtca gtagtatatt ctacatcaaa
     4141 tgagccaaga cctttcaatg catcccaacg gataatttca ggatgaccgg gaacaaaacc
     4201 ttcgccaccc ataggatagg taacagtgat gtcatcattt ctgaactcat aaactacata
     4261 ataagtctgt ggtccttgtg gaatagcaaa tcctttgagt gaaacaacat agttacctgc
     4321 tgcagggttg tctaatgtaa cttgttccat gttgttcaga tggtcaatgc ctcttgttgc
     4381 aggtgcattc agattagcgg cattgggagt aggatcaaga acccatggat tccatactgc
     4441 atttgatggg tcggttactt gcatgtcaat gtcgttaact aaataagtag atgatgccgg
     4501 tgagccacct acatcatgcc agtaaaccat gacacgcatt tgcgaagtac ctgcaggaac
     4561 gttaatagaa taagtcaatg agtcactctg attaatactg tcagtaaaat aacgtgcatc
     4621 ttcaatagta gtaacagcac gcagtgcatt tactcttccc caaccatatt tgtaatcagg
     4681 gccagggttt ccgatatctt cagcaccatt gagcaagcaa gctttaatta aagcagtagg
     4741 tgcatcggta gcagaattaa tttgcttata agcttgaata agttgtgtgc agatacctgc
     4801 aatgcccggg caggctgcag aggtgccacc accaacctga taagtattat tttcatctgt
     4861 tgacatttgg tcgcggccat tggcagcaat atccggtttt actctgccgt cagaagcggg
     4921 accaatactg cttgaaggat caataacctc caaagcatca agattggcta cagcaattac
     4981 attttttccc tgcttgtaac ctcctgtaat atttccccaa ttacctccgg caccatagcc
     5041 acaattggta ccattgttat ttcctgcaga gaaaacaaat tcaagttgtc cattgtcatg
     5101 aatagtctga tcgccaaatt gtgtgtcggt agtatattca ttacatccct gcgaatagct
     5161 ggttgaagca accactgttc caagattgtt atagttggca actgcatcaa taatttgtgg
     5221 gtagcttcca atatcaaaaa catgcagttg aactccattg gccatacccg gatatgcagg
     5281 atttaaattg cctgcacctg caagaattcc gcctgtcatg tcaccatgcg aaggaccaac
     5341 tgttgttgtg taattggtaa tccttccggt aaaatcaatg tgtgggccaa caaatccatc
     5401 atcggcaagt gctgcggcta ctccgcttcc attgtattta cgtcccatca acaagtcgtt
     5461 gttgatgaca ttgctgcggt gcagactacg tccttccaaa tcatctttaa cagatggtgg
     5521 cgaggttaaa tctataaaaa acaaccaagg ctgttgtgaa atagtggcaa cagattgttg
     5581 ctgcatgcgc acctcaacca tattatttat ttcagttgaa cttaaaattt tgccataggt
     5641 agcggcaatc tgttgtgctt gttgtattga aatgtttttc tgaaactgaa gtctcacatc
     5701 aaccattcct gcagaaggaa cagcgtattt aggataatca cctaacatgc tgatagaaag
     5761 tttctgttca gaagattgat ttgttacact tctgacatta aatcctgaga gcaattgttt
     5821 gttaaaacta attggaattg ctgcattaaa agcattcttt ggcaagtact ccaacagctt
     5881 aattcctgca gtttccatta cagctttttc tgcttgagta ggtacagtat aaaactgtat
     5941 gattcggtag taataaccat tataaacatc gttttctgat actttttcct ggataaaaga
     6001 gtttaggttt tctgccggca aaacagcttt acctaaaaac atcaggcgat agtctgtttg
     6061 cgccatagcc attgttgtag tac
//
LOCUS       DAORQT010000094         6067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2131_length_6067_cov_10.040752, whole genome shotgun sequence.
ACCESSION   DAORQT010000094 DAORQT010000000
VERSION     DAORQT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2131_length_6067_cov_10.040752"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /gene="gpmI"
                     /locus_tag="PK134_04585"
     CDS             <1..799
                     /gene="gpmI"
                     /locus_tag="PK134_04585"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRB38157.1"
                     /translation="PVATIQDGDAVICFNFRTDRCREITMALTQQDFHEQNMHKLNLE
                     YVTMTVYDDKFTGVKTIFSKDNLHNTLGEVLQNANKSQIRTAETEKYPHVTFFFSGGR
                     ENEFRGEKRILIPSPKVATYDLQPEMSALPLKDALVKELKKGETDFICINFANADMVG
                     HTGVFSAAVKAVETVDACVKEIVQCGLEQGYSFLITADHGNADYMINDDGSPNTAHTT
                     NPVPLFLIQPQHEFNLKNGILADLAPTILQLMQVKQPSEMTGKSLLS"
     gene            907..1812
                     /locus_tag="PK134_04590"
     CDS             907..1812
                     /locus_tag="PK134_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38158.1"
                     /translation="MKKITLLLLLLSVNQLYGQSRTFFSLSGGILYYNGDLNDKSPVP
                     STKIFRNYGNAAIGIHLTRHVNLQLNYYHGSIEGADSLTKERDNKQRNLSFETRIDEI
                     SFQLSASLFSNRTKKLAIPYVFAGGGLFFFNPRARYYGSNPDYYGKLVSLQPLGTEGQ
                     YITEGDYPKPYKLYQAVFPIGIGTYIRINPKWRLKVEFSEHFTLTDYLDDASGKYPNM
                     EALSLTPKGELASYFADRRLNRSQDPSGRNRSNATKNDNYIHFGIGLVYNPQAQHPTR
                     YRGPGFWKKLFSGRKGWWGNAGNGL"
     gene            1891..2712
                     /locus_tag="PK134_04595"
     CDS             1891..2712
                     /locus_tag="PK134_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB38159.1"
                     /translation="MKILVPVDFTDITEKGFKYALSLPFANEIILFHAVDDIKKSKEA
                     QQQLSNITNKYSIPAHLTVQGKVKQGNIFETLGDVSKEVNADLIVMATHGIKGIQHFL
                     GSHAMKLITHSKTPFIVVQQKDYSPLKKMLVPVDFTKEVKQELPAVIQYAQLFNASVL
                     LLKQNNHDPYIQKRSDLNTGYFEDELDNAKIKYELIEQTFSLDDKYEVITKQAAAHNA
                     DIIVTTIEPDLDVADYVIGVEEQKIVANDLQLPVLCVNIRHFTIAGDQFSVTVQQ"
     gene            2858..3133
                     /locus_tag="PK134_04600"
     CDS             2858..3133
                     /locus_tag="PK134_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38160.1"
                     /translation="MSLVTCKEFNNLSHDEQTWYIWNGAIHLHVREVYGYRVNLFYFN
                     EFYIELWYSINENSIKKIKSFDNSALLAPYLETIKIDSLLNIDSGFE"
     gene            complement(3136..3813)
                     /locus_tag="PK134_04605"
     CDS             complement(3136..3813)
                     /locus_tag="PK134_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRB38161.1"
                     /translation="MKRFRSLFGLKIMVVMVLLFSSFKAPDFTLKLAKLKYAGGGNWY
                     VSPTALHNLAVFCNQNINTNISPEEAVVEPGSPDLFNYPFVHMTGNGRFVFNNQEAEN
                     LRKYLIAGGFLNINDSYDMDKFVRPEMKKVFPELDFVELPFNHPIYHQRYNFPNGLPK
                     IHEHNGKAPQGFGLIYQGRLVCFYEYESDIGDGWEDPEVHNDPPEMRQKALQMGANIL
                     QYVFSGQ"
     gene            3912..>6067
                     /locus_tag="PK134_04610"
     CDS             3912..>6067
                     /locus_tag="PK134_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRB38162.1"
                     /translation="MTLRILLVAIFAPLMLNAAHDHNGFDRNEDPNLKVFVKDRKRLP
                     DVNRQESLAQQKSWQQFSEQNKGWSVVFNEETGLPHNAFGKPISVGLPLDAQSTAWSF
                     ISTKLNSFGLPLNDLYFRNTAQNKKYFYVNYIQRYNGLEVLWSKVQIKITKDGKVNQF
                     ICDAYKDITVNINPTLSTQAASAAAINGLNLTLNGTTVNSDLKVLPVPDAAKNIYHLV
                     YEVTVKATDAENFPREYYTLVDAHNGEVLYRHNKINYFAANTDVNVTATVYPTQPYLP
                     TAVEPLANMNVTISGVPFLTDANGYLGLPNTSPVTATFKLEGSWANVKTGATTPQFTA
                     TLNPGANNITFSSANIKELSAYNSVNEIHDYYVTKMMGSGAETVMDIQMQTIVDVSGN
                     CNAFYNGDLNFYAAGGQCNATSIINDVVYHEYGHGINYDLYSYYGGIFSNGALGEGYA
                     DTWANGLTEDPVLGIGFYSGNATGFVRRYDQGRKVYPDDIMGEVHADGEIIAGAWWDV
                     GLNFNSVQQRMDLYVQTFAATIDYPDGQEGQLYTDILVEALTIDDNDGDLSNGTPNYC
                     AISSAFGIHGINMFVVGKLITHNDVLSAPGQQNITLNASGPASFGNGTGNSVNGYYSL
                     NHSATWNPISFTYNGTIFQGSIPGQPNGTIVSYYLDATDQCGTHFSLTPPNVTDSNPN
                     LPYYVLVGYNLVASDYFDTGNSQGWTLGMPGDNATTG"
BASE COUNT         2010 a         1148 c         1107 g         1802 t
ORIGIN      
        1 acctgttgca accatacaag atggtgatgc agtaatctgt ttcaatttcc gtactgaccg
       61 ctgccgtgag ataactatgg cacttacaca acaagatttc catgaacaga atatgcataa
      121 gctaaatctg gagtatgtta ccatgactgt atatgatgac aaattcacag gtgttaaaac
      181 cattttttcc aaagataatt tacacaatac attaggcgaa gttttgcaga atgcaaataa
      241 atctcagata cgaactgccg aaacagaaaa atatccgcat gttactttct ttttttcagg
      301 aggacgagag aatgaattca gaggagaaaa acgcatcttg attccatcac ctaaggttgc
      361 cacttacgac cttcaaccgg agatgagtgc tttaccatta aaagatgcct tagtgaaaga
      421 actaaaaaaa ggcgaaacag attttatctg catcaacttt gccaatgcag atatggttgg
      481 gcatactggc gtattttccg cagctgtaaa agcagtagaa acagtagatg cctgtgtaaa
      541 agaaattgtg caatgtggtc tggagcaagg atattcattt ttaattacag cagatcatgg
      601 taatgccgat tatatgatta acgatgatgg ctcaccaaat actgctcaca caacaaatcc
      661 tgtacctctg tttcttatcc aaccacaaca tgaattcaat ttaaaaaacg gcattctagc
      721 cgaccttgca cctactattc tgcaactgat gcaagtgaag caaccctctg aaatgactgg
      781 aaaatcgttg ctttcatgaa caacattact taacataacg ctgtgtaaaa cataagtata
      841 acagtgggcg ttttagaatt gtagttttat gtaagagact acttttgcat tcaatcggga
      901 gtgagcatga aaaaaattac cctgttactt ttactactat ctgtaaatca actttacggg
      961 caaagccgca ccttcttttc actgtcagga ggtattcttt attacaatgg cgaccttaac
     1021 gataaaagcc cagttccatc aacaaaaatt ttcagaaact atggtaatgc tgccataggt
     1081 attcatctga caaggcatgt aaacttacag ctaaactact atcatggaag cattgagggt
     1141 gctgactctt taactaagga gcgtgataac aaacaacgta acctcagctt tgaaacacgc
     1201 attgatgaaa tttcttttca actgagtgca agtttattct ctaaccgcac aaaaaaactt
     1261 gccataccct atgtatttgc cggtggtggt ttatttttct ttaatccaag agcacgctat
     1321 tatggcagta accctgatta ttatggaaaa ctagtttcat tacaaccttt aggcacagaa
     1381 ggacaataca tcactgaagg cgattatcct aaaccataca aactttatca ggctgtattt
     1441 ccaataggca ttggcacgta tatcagaatc aaccctaaat ggcgtttgaa agtagaattt
     1501 tctgaacatt ttacactgac cgattactta gatgatgcca gtggtaaata tcccaacatg
     1561 gaagccttat cactaacacc aaaaggtgaa ttggcttctt attttgcaga cagaagatta
     1621 aacaggtctc aggatccttc aggaagaaac cgctcaaatg caaccaagaa cgacaactat
     1681 attcattttg gtataggttt ggtttacaac ccacaggctc aacatccaac ccgctacaga
     1741 ggtcccggat tctggaaaaa acttttcagc ggacgcaaag gctggtgggg caatgccggc
     1801 aatggccttt aagtaaaaca atcatccttt cgtcatttat tgctttaact tttcatgttg
     1861 tggagtgtta tcattctaaa taacatgtat atgaaaattt tagttccggt tgactttacc
     1921 gatattactg aaaaaggatt taagtatgca ttgtccttac cttttgcaaa tgaaataatc
     1981 ctttttcacg ctgttgatga cataaaaaag tctaaagaag cacagcagca actcagcaac
     2041 attacaaaca aatattccat tcctgcgcat ctgacggtac agggaaaagt aaaacaagga
     2101 aacatttttg aaacattagg ggatgtttca aaagaggtta atgccgattt aattgtgatg
     2161 gctacacatg gtataaaagg tattcaacat ttcttaggca gccatgccat gaagttgata
     2221 acacattcaa aaacaccttt cattgtggtg caacagaaag attacagtcc actgaaaaaa
     2281 atgcttgttc cggttgattt caccaaagaa gtaaaacaag aactacctgc agtaattcaa
     2341 tatgcacagc tttttaatgc aagcgtattg ctactcaaac aaaacaacca cgatccttac
     2401 attcaaaaaa gaagcgatct caataccggt tattttgaag acgaactcga taatgcaaaa
     2461 attaagtacg aattgattga acagacattt tcacttgatg ataaatatga ggtgattacc
     2521 aaacaagctg ctgcgcataa tgccgatatt attgtaacca ctattgagcc cgacttagat
     2581 gttgccgatt atgtaatagg tgttgaagaa caaaaaattg tagctaacga tcttcaattg
     2641 cctgttttat gtgtaaacat caggcatttt acaattgccg gtgatcagtt cagtgttact
     2701 gtacagcagt aatgtttaag taccattcgt tcaacttcgt tccattgaca ttagccaatt
     2761 aaaaaacgat tttgaccaaa aggtttatcc atctgcaaga ataagactgc atattggttt
     2821 aacaattcaa caaaaaacaa tcgttaaact aagaagtatg tcgttggtaa cctgcaaaga
     2881 atttaataac ctgtcacatg atgaacaaac ctggtatatc tggaatggtg caattcatct
     2941 tcatgtaaga gaagtttatg gctacagagt aaatctgttt tatttcaacg aattttacat
     3001 tgaactttgg tacagcatta atgagaacag cattaaaaag ataaagtcgt ttgacaattc
     3061 cgcactgttg gccccttacc tagaaacaat taaaattgat tcattactga atattgattc
     3121 aggttttgaa taatattact gtccgctaaa aacgtattgc agaatattag cacccatctg
     3181 caaagctttt tgacgcattt caggtgggtc gttatgaacc tcggggtctt cccaaccatc
     3241 gccaatatca ctttcatatt cataaaaaca gactaaccta ccctgatata tcagtccaaa
     3301 accctgtggt gcttttccat tatgttcatg gattttaggc aatccattcg gaaaattata
     3361 ccgctgatga tatattggat ggttgaaagg taattctacg aaatcaagtt caggaaaaac
     3421 ttttttcatt tcaggtcgga caaacttatc catgtcgtag ctgtcgttga tatttaaaaa
     3481 tccaccggca atcagatatt ttctcaggtt ttcggcctct tgattgttga acacaaaacg
     3541 accatttcct gtcatgtgca caaatggata attaaacaag tcagggctac ccggttctac
     3601 tactgcctct tcaggactta tgttggtatt aatattctga ttacagaaaa cagccagatt
     3661 atgtaatgcc gtagggctga cataccaatt accaccacct gcatatttga gcttggcaag
     3721 tttcagtgta aaatcaggtg ctttaaatga agaaaacaac agtaccatca ctaccatgat
     3781 tttcaaccca aaaagactac ggaaacgctt catttgaccc caaaaatatg tttaatcagt
     3841 gacacaatct tatttgcacc ttaattttat atgaataatt ttgtccttcc aaaaaatcaa
     3901 atctatttaa tatgacattg cgaattttac tcgttgcaat ttttgcaccg ctgatgctta
     3961 atgcagcaca tgatcacaac ggtttcgacc gtaatgaaga ccccaatctt aaagtatttg
     4021 ttaaagacag aaaacgttta cctgatgtaa accgtcagga aagtcttgcg caacaaaaat
     4081 cgtggcaaca gttttcggaa caaaacaaag gatggtctgt agtttttaac gaagaaaccg
     4141 gattaccaca caatgctttt gggaaaccaa tttctgtggg actgccttta gatgctcaat
     4201 ctactgcttg gtcgtttatc agtacaaagc ttaattcttt tggtcttccg ttaaacgatt
     4261 tatatttcag gaatacagca caaaacaaaa agtactttta tgtaaactac attcagcgtt
     4321 ataatggact tgaagtatta tggtcgaaag tgcagataaa aattaccaag gatggaaaag
     4381 ttaatcagtt tatttgcgat gcatataaag acattactgt aaacattaat ccaacattga
     4441 gtacacaagc tgcttctgct gctgcaatta atggtctaaa tttaacatta aatggcacca
     4501 cagtaaacag tgatttaaaa gttttgcctg tgcctgatgc agcaaaaaat atttatcatt
     4561 tggtttatga ggtaactgtc aaagcaacag atgcagaaaa ttttccaaga gaatattata
     4621 cgcttgttga tgcacataat ggcgaagtgc tttatcgtca taataaaatc aattattttg
     4681 ctgccaacac agatgtcaat gtaacagcaa cagtttaccc aacacagcct tatttaccta
     4741 ctgctgttga gccattagcc aacatgaatg ttaccatttc cggtgtacct tttctgactg
     4801 atgcaaatgg ttatttagga cttcctaaca cttcacctgt aacggcaact tttaaattag
     4861 aaggtagttg ggcaaatgta aaaaccggtg ctacaacacc acagtttaca gcaactttaa
     4921 atccgggtgc taacaacatc acattcagta gtgcaaatat caaagaactt tctgcataca
     4981 attctgtgaa tgaaattcat gattattatg taaccaagat gatgggttca ggcgctgaaa
     5041 cagtaatgga tattcaaatg caaaccatag tagatgtttc aggaaactgt aatgcatttt
     5101 ataatggcga cttaaacttt tatgctgcag gtggccaatg taatgcaact tctattatta
     5161 acgatgtggt ttatcacgaa tatggtcatg gaataaacta tgacttgtat tcctactatg
     5221 gaggtatatt cagcaatggt gcacttggag agggatatgc agatacatgg gctaacggat
     5281 taacagaaga tcctgttttg ggaataggtt tttattccgg taatgcaaca ggttttgtaa
     5341 gacgttatga tcagggaaga aaagtttatc cagatgatat tatgggtgaa gtgcatgccg
     5401 atggtgaaat tattgcaggt gcctggtggg atgttggttt aaactttaac agtgtacaac
     5461 agcgtatgga tttatatgta cagacttttg cagcaactat tgattatcct gatggacaag
     5521 aaggacaact ctacaccgat attttagttg aagcattaac tattgatgat aatgatggag
     5581 acctgagcaa tggaacacca aattattgcg ccatttcttc tgcctttggc atacatggaa
     5641 taaacatgtt cgtagtagga aaattaataa cccataatga tgtactcagc gctcccggtc
     5701 aacaaaacat tacactcaac gcttccggtc ctgcaagttt tggaaatggc acaggtaaca
     5761 gtgtaaacgg atactacagt ctgaaccatt ctgctacatg gaatccaatc agctttactt
     5821 ataacggaac catttttcag ggaagtattc ccggtcaacc taacggtaca attgtttcat
     5881 attatcttga tgcaacagat caatgtggaa cacactttag tttaacacca cctaatgtaa
     5941 cagattccaa tcctaatctt ccttactatg ttttggttgg atataattta gtggcaagtg
     6001 attattttga tacaggcaac agtcaggggt ggactttagg tatgccgggt gataatgcaa
     6061 caacagg
//
LOCUS       DAORQT010000095         5994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2170_length_5994_cov_11.965988, whole genome shotgun sequence.
ACCESSION   DAORQT010000095 DAORQT010000000
VERSION     DAORQT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_5994_cov_11.965988"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="cls"
                     /locus_tag="PK134_04615"
     CDS             <1..1060
                     /gene="cls"
                     /locus_tag="PK134_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRB38163.1"
                     /translation="LNAIASAKHHIHIEYYIFENDSIGKSVIEALIAKVGEGITVRFI
                     YDDFGSHALNRKILKRMKDGGINLFPFSRVRLFALANRLNYRNHRKIIVIDGNIGFVG
                     GINISERYINSEEQGKKLYWRDTHLRIEGAGTYYLQYLFLCDWNFCAQSQLQPSREFF
                     PSPTLLNNKGNKIVQIAASGPDSERPTILFSLLQAISLASKEILITTPYFIPGESILD
                     ALMVAALGGVSVKILVPGISDSRIVNAAAFSYYDSLLNAGAEIYLYQKGFIHSKTLVA
                     DDKIAMVGTANMDHRSFDLNFEVNAIVYDETFAKELKREFYKDLKDAESIDVKAWNNR
                     PIIKQLGEKCARLISPML"
     gene            complement(1080..1682)
                     /locus_tag="PK134_04620"
     CDS             complement(1080..1682)
                     /locus_tag="PK134_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRB38164.1"
                     /translation="MNAECTPFNSNDKAVENIIVNRARHLFMKVDIKSVSMDDIANAC
                     GISKKTLYKCFDKEGLVKTVTQQQVHIFENEIKSILQNNKDVVQELTNLLLFTINASQ
                     EISLPFIDSLKTIKPEAYQLILNFTKNIQQTFINNIKKGMSQGYYRSDIEPDVVVQLH
                     FLQLWSLMKQQMLSKSLHWGEELNKMFLFGILSPKGLKTL"
     gene            complement(1695..4883)
                     /locus_tag="PK134_04625"
     CDS             complement(1695..4883)
                     /locus_tag="PK134_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB38165.1"
                     /translation="MKITEISINRPTMVVVVFTILGILGLISYSSLNYELLPKFSTSM
                     VTISTVYPGAGPDEVQNTVTKKIEDAVSSMENIKKLTSRSMEGVSIVTVEMNVGADAN
                     RSLEDAQRKINAMLSDLPSDAKTPTLSKVSVDDLPIITLSVTADMDSREFFDLLDQRL
                     APRLSRLPGVAQVQMIGGTEREFQINVDAGKLNAQKLSLLQLRQTITNANVDYPTGKI
                     QTTDNLIAIRLSGKYADVDMLRNLVIKTSDDGSQVRLKDVADVRDGQKDVNKLARQDQ
                     KETIVVQITKQSDANAVTVSKEVHNQIAGIEKEYASNNLKMFVASDSSEFTLESANDV
                     VFDLFIAIILVAVVMLLFLHSIRNSLIVMISIPLSLIITFAGMYVMGYSLNLMSLLAL
                     SLVVGILVDDAIVVLENIYRHMEMGKNKVRAAFDGVKEIGLTVTSITLVIVVVFLPIS
                     LTNSLVSMILRQFCITVVISTLLSLLVSFTVVPWLSSRFGKLEHLTGKNMFQKFILWF
                     ERQIDKFTEWITGILRWSLGHKRYVLIGTIVLLVASFMLLAKGFIGGEFIPKGDRGQF
                     IVTIEMPKDVALEVTNQMTMRAENYLAKQDLVKSLTTTVGKSSDGGFGTSSSTPYKSE
                     ITVTMIDENLRSEPSSMYAAKIKKELAAQLAGAKITTSPVSIMGTAERASIDLVVTGT
                     EQDSAMAFAKKALLALQKIDGVSDPKLSAEDGNPEVRVTLDRDKMALLGVNMDVVGGT
                     MQTAFSGTDQDSKLKFREGEYEYDINLRYDNFNRKNADDVRNIMFVNNKGQNIRLSEF
                     ATISETSGPTVLERRDKLASVNVQAQVIGRPAGSVTTDFTESLKKLDKPAGVNYIFGG
                     DAENQGNAFGTLGLALLLSIIIMYLIMVALYDDYVRPFVVLFSIPLSLIGALLALALT
                     NNTLNIFSILGVIMLIGLVAKNAIMLVDFANQMKEKGESTDEALVHANNARLRPILMT
                     TIAMVIGMLPIALASGGVAAIKHGLAWVIIGGLISSMFLTLIVVPVIYKIMDSVSARF
                     GWGDLNKKKIVRELMHAENTNTADNHSISHVI"
     gene            complement(4921..5982)
                     /locus_tag="PK134_04630"
     CDS             complement(4921..5982)
                     /locus_tag="PK134_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB38166.1"
                     /translation="MKKVFIYGGTLIVALALIVWKLNANKKSDAARIEVVRESSSGAV
                     PVQILAVKSSNVDLSFDENGNFEAVNQIELTAEIAGRVSELYVKEGSVVKAGQAIAKI
                     ENEILNANQTEAKARLEQLRLNLNRYEQAYKTGGVTQKQVDDARLELESAQAKNVQAN
                     KNLYNSVVKAPVNGIINTRYVEVGTYLASGNKIVEIVDDAKLKLTVNVNEYQIIQLHT
                     GDKVEVTASVYPEVTYQGVITFIAAKSDASLNYPVEVEINNIAGKALKAGMYGTAHFK
                     PEIKNASLMIPRSAFQGGINSNLIFINDNGKAKLQKVVAGNVHGNMVEIREGLKEGDQ
                     VIVTGQINLVDGKQISIVK"
BASE COUNT         1852 a         1153 c         1011 g         1978 t
ORIGIN      
        1 ccttaatgca atagcatcag caaaacacca tattcatatt gaatattaca ttttcgaaaa
       61 cgatagtatt ggtaagagtg ttatagaagc tttaatagct aaagttggcg aaggaataac
      121 ggtgaggttt atttatgatg actttggaag tcatgcatta aataggaaaa tactgaaacg
      181 gatgaaagat ggcggaatca atctttttcc tttttcaaga gtaagattat ttgcattagc
      241 caatcgcctt aactacagaa atcatcgaaa aattattgta atagatggta atattggatt
      301 tgttggcgga attaatatta gtgaacgtta tataaattca gaagagcaag gtaagaaatt
      361 gtattggcgc gacacacatc tgagaataga aggtgcaggc acatactatc tgcaatatct
      421 ttttttatgt gattggaatt tctgtgcaca gtcacaattg caaccttcaa gagaattttt
      481 tccatcacca actttattga ataacaaagg gaataaaatt gttcagattg cagcaagtgg
      541 accggattct gagcgaccca caattttatt ttcattacta caggctatca gtctggcctc
      601 aaaagaaata ttaattacaa caccttattt tattccggga gaaagtattt tggatgcttt
      661 gatggtagct gctcttggtg gagtgagtgt taaaattctt gttcccggta tttctgattc
      721 aagaatagta aatgcagctg cattttcata ttacgatagt cttttaaatg caggtgcaga
      781 aatttatttg taccagaaag gctttattca ctctaaaaca ttggttgctg atgataaaat
      841 tgcaatggtt ggaacagcaa atatggatca tcgtagtttt gacctcaatt ttgaagtcaa
      901 tgctattgta tatgacgaaa catttgcgaa agaactaaaa cgtgaatttt ataaagattt
      961 aaaagatgct gaaagtatag atgtgaaagc gtggaacaac cgccctatta ttaaacaatt
     1021 gggagaaaaa tgcgcacggt taatttctcc aatgttataa tcattaaaaa gcaaaggcac
     1081 tataaagttt ttaacccttt tggagataaa ataccaaaca gaaacatttt atttagttct
     1141 tcaccccaat gtaaagattt gctaagcatt tgttgtttca ttaaactcca tagttgcagg
     1201 aagtgcaact gtacgacaac atcgggctca atgtcggaac gataatatcc ttgcgacata
     1261 ccttttttaa tattatttat aaaagtctgt tgaatatttt ttgtgaaatt tagtatcagt
     1321 tgatatgctt ccggttttat tgtttttagt gaatcaataa agggaagaga aatttcttgc
     1381 gaagcattta ttgtaaataa aagaaggttt gtcagctcct gtacaacgtc tttgttattt
     1441 tgcaagattg atttaatctc gttttcaaat atatgaacct gttgttgtgt aacagttttt
     1501 accaaacctt ctttatcaaa acatttatat agtgtttttt tggaaatgcc acatgcattt
     1561 gcaatatcat ccatacttac tgactttata tctaccttca tgaacaaatg acgcgctcta
     1621 ttgacaatta tattttcaac tgctttatcg tttgaattaa atggagtgca ttcagcgttc
     1681 atgaatgttt atgcttaaat tacatgagat attgaatggt tatctgctgt atttgtattt
     1741 tcagcatgca tgagttcgcg tactattttt ttcttattca aatcgcccca accaaaacga
     1801 gcagaaacgc tatccataat tttataaata accggaacaa ctattaatgt caggaacatg
     1861 gatgaaatta accctccgat aattacccag gccaaaccat gtttgatagc ggctacacca
     1921 cctgatgcca atgcaatagg aagcatacca attaccatag caatggttgt cattaaaatt
     1981 gggcggagac gtgcattgtt ggcatgtacc aaagcttcgt ctgtagattc tcctttttct
     2041 ttcatttggt tagcaaagtc aaccagcatg atggcgtttt ttgccactaa accaatcaac
     2101 atgataactc ccagaatact gaaaatattg agtgtgttat tagtgagtgc taaagcgagc
     2161 aatgcaccaa tcagagaaag cggaatagag aacaatacaa caaacggacg cacataatca
     2221 tcatacaaag caaccataat cagatacatg ataataatag aaagtaataa tgccaaacct
     2281 aatgtaccaa atgcatttcc ctgattttcg gcatcgccac caaagatgta attgacaccg
     2341 gcaggcttat ccaatttttt cagactttcg gtaaaatcag tggttacaga accagcaggt
     2401 ctgcctatta cttgtgcctg tacatttact gatgcaagct tatctctgcg ctccaataca
     2461 gtaggtcccg aagtttcaga tatagtagca aattctgaca atcgaatgtt ctgaccttta
     2521 ttgtttacaa acataatgtt gcgcacatca tcagcatttt ttcggttgaa gttatcgtag
     2581 cgcaggttaa tatcatattc gtattctcct tcgcggaatt taagtttact atcctgatcg
     2641 gtaccactaa aagctgtttg cattgtgccg cctacaacat ccatattcac acccagtaga
     2701 gccatcttat cacgatcaag tgttactcgc acctcggggt taccatcttc tgcagataat
     2761 tttgggtcag acacaccatc aattttttgt aatgcaagta atgctttttt agcaaaagcc
     2821 atagcgctat cttgctcggt tccggtaaca accaaatcaa tagaagcacg ttcggcagta
     2881 cccatgatgc ttacgggaga agttgttatt ttagctccgg ctaattgtgc agctaattcc
     2941 tttttgattt ttgcagcata catgcttgaa ggctcgcttc gcagattttc atcaatcata
     3001 gtcactgtaa tttctgattt gtaaggtgta gatgatgaag tgccaaatcc tccatcgctg
     3061 ctttttccta ctgttgtggt taatgacttt accaaatcct gtttagctaa gtaattttca
     3121 gcacgcatag tcatttgatt ggttacttcc aaagcaacat ctttaggcat ttcaatggtt
     3181 acaataaact gtccgcgatc tcctttaggt ataaactctc caccaataaa cccttttgca
     3241 agcaacataa aagatgcaac cagcaataca attgtaccaa taagtacata gcgcttatga
     3301 ccaagcgacc aacgtaatat gccggtaatc cattctgtaa acttatctat ttgacgttcg
     3361 aaccacagaa taaatttttg gaacatgttt ttacctgtca gatgttcgag ttttccaaag
     3421 cgtgatgaca accaaggaac aacagtaaac gacaccaaca aacttaacag tgttgaaatt
     3481 actactgtta tacaaaattg acgcaatatc attgacacta aactattggt tagtgatatt
     3541 ggaagaaata ctactacaat tactaatgtg atggaggtaa cagtaagacc aatttctttt
     3601 acaccatcaa aagcagcacg aactttattt ttacccattt ccatatggcg gtaaatattc
     3661 tccaacacca caattgcatc atcaacaaga atacccacca ccaaggaaag ggcaagcaaa
     3721 gacatgaggt taagtgaata acccattaca tacataccgg caaatgttat tatcaatgac
     3781 agcggaatgg aaatcattac aatcagcgag tttcgtatgc tgtgcaagaa caacaacatt
     3841 acaaccgcta ccagaataat ggcaataaat aaatcgaata caacatcgtt ggccgattcc
     3901 agagtgaact ctgatgaatc agatgctacg aacattttta aattgttact tgcatattct
     3961 ttctcaattc cggcaatttg attgtgtact tctttactaa cggttacagc attagcatca
     4021 gattgtttgg taatttgtac tacgattgtt tctttctgat cctgacgtgc caatttattt
     4081 acatcttttt gtccatcgcg tacatctgca acatctttca agcgtacttg cgaaccatca
     4141 tcactagttt taattaccag attgcgcaac atatccacat cggcatattt tccggaaagg
     4201 cgtatggcta tcagattgtc ggtggtttga attttgcctg tgggataatc aacgttggca
     4261 ttggtaatag tttgtcgtaa ttgtaacaac gatagttttt gtgcattaag tttaccggca
     4321 tctacattga tttgaaattc acgctcagtg ccaccaatca tttgtacttg tgctacaccc
     4381 ggtagtcgtg aaagacgagg tgccagccgt tgatctaaaa gatcaaagaa ttcacgcgaa
     4441 tccatatcgg cagttactga caatgtgata ataggtagat catcaacaga aactttactg
     4501 agtgttggtg ttttagcatc acttggcaaa tccgacaaca ttgcatttat tttacgttgt
     4561 gcatcttcca aacttctgtt ggcatctgct cctacattca tttcaactgt aactatagaa
     4621 actccttcca tagagcgtga tgtaagtttc tttatgttct ccatagaaga tactgcatct
     4681 tcaattttct ttgttacagt attctgaacc tcatcaggac cggcacccgg atataccgtg
     4741 ctgatggtta ccattgaagt actaaattta ggcaataact cataattgag agagctataa
     4801 ctgataagac ccagtatgcc taatattgta aaaacgacca caaccattgt aggtctgttt
     4861 attgatatct ctgtaatttt catttattcg atttttaaat gttagctttt aaaacagaac
     4921 ttattttaca atagaaattt gtttgccatc taccagatta atttgtcctg taacaattac
     4981 ctgatctcct tcttttagtc cttcacgaat ttcaaccata tttccgtgaa catttccggc
     5041 aactactttt tgcaattttg ctttgccatt atcattaata aaaattaagt tactgttaat
     5101 accaccttga aatgcagagc gcggtatcat taaagatgca ttttttattt caggcttaaa
     5161 atgtgctgta ccatacatgc cggcttttag tgctttgcct gctatgttat taatttctac
     5221 ctcaacaggg taattcaatg aagcatcact tttagcagca atgaaagtga ttacaccttg
     5281 atatgttact tcaggatata ccgatgcagt tacttctact ttatcaccag tgtgcaattg
     5341 aataatctga tattcattta cattaacagt gagttttaat tttgcatcat caacaatttc
     5401 aacaatctta ttgccggaag ccagataagt tccaacctct acataacggg tattaataat
     5461 tccatttaca ggagctttca ctacagagtt ataaagattt ttatttgcct gaacattttt
     5521 tgcttgtgca ctttctaatt ccaaacgggc atcatcaact tgtttctgtg ttacacctcc
     5581 tgttttatat gcttgctcat aacgatttaa gtttaatctc aactgctcca accgtgcttt
     5641 tgcttctgtt tgatttgcat tcagaatttc attttcaatt tttgcaatag cctgtccggc
     5701 tttcactaca cttccttctt ttacatataa ctcactaacc ctacctgcaa tttctgctgt
     5761 taactcaatt tgatttacgg cttcaaaatt tccgttctcg tcaaatgaca aatcaacgtt
     5821 agatgattta actgctaaaa tttgtacagg aacggctcca cttgagcttt cacgcaccac
     5881 ttcaatgcgt gctgcatctg attttttgtt tgcatttagt ttccaaacaa taagggcaag
     5941 tgccactatt aatgtacctc cgtatataaa tacctttttc ataatttatt attc
//
LOCUS       DAORQT010000096         5968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2190_length_5968_cov_5.325554, whole genome shotgun sequence.
ACCESSION   DAORQT010000096 DAORQT010000000
VERSION     DAORQT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_5968_cov_5.325554"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PK134_04635"
     CDS             complement(<1..801)
                     /locus_tag="PK134_04635"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRB38167.1"
                     /translation="MNFELTILGSSSATPIHQGHPTAQVLNIRERFFMIDCGEGTQGQ
                     FLKYKFKLNKLSHIFISHLHGDHYLGLVGLLSTMHLQGRTTEIHIYGQPELMDVIELQ
                     LRLSQTRLRYQLIFHPVQHYAPAIIYEDDDMLIKSIVLNHRIPCTGYLFSEKPKPRKL
                     IISKLQQHNIPFSFYGRLKDGFDYEDDEGKIIPNTELTISSTAPRSYAFCSDTCYDES
                     IIDEIKGVNLLYHEATFMNDLAERAKTTFHSTSLQAATIAKKAGVDKLL"
     gene            complement(801..1571)
                     /locus_tag="PK134_04640"
     CDS             complement(801..1571)
                     /locus_tag="PK134_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38168.1"
                     /translation="MIKKLLFSLMFISMSGIAVSQCTPDPTIIDPGVYPDSATGLSNG
                     MVGVAYNQVIQVRTFFDTTATINIGGVPFNANFELDSITILGVSGLPPGLTYACNPGT
                     CQFLASSNGCLLLSGTPVTAGVYPITVDIIARGKITNLGNISYGQPMSVTYYTITIDT
                     NSGVIEMLPKDKLSMSQNLPNPFKNKSVVYFNSPVNTKLDFKIYNILGKNIFSETISA
                     KRGVNTYTINAENLSSGIYMYSINTGTANITRRMVISK"
     gene            complement(1589..1945)
                     /locus_tag="PK134_04645"
     CDS             complement(1589..1945)
                     /locus_tag="PK134_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRB38169.1"
                     /translation="MHYTLDKKEKYAVLKLHEQKLNTLISAELKSELLILNSQGFIHI
                     VLDLSDTQYCDSSGLSAILVGNRICRNANGNFIVTEISDPVKKLINISQLDSVLTIVP
                     KIDEALALINKAASVS"
     gene            complement(2027..2956)
                     /locus_tag="PK134_04650"
     CDS             complement(2027..2956)
                     /locus_tag="PK134_04650"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRB38170.1"
                     /translation="MGKLSVKHLIGIKDLKPEDINLILDTAQTFKEVINRPIKKVPSM
                     RDVTVVNLFFENSTRTRLSFELAEKRLSADILNFTASSSSVSKGETLIDTVNNILAMK
                     VDLVVMRHPKPGAALFLSQKVNSVIVNAGDGTHEHPTQALLDAFSIREKLGTLKGKKV
                     VIVGDILHSRVALSNIYCLQKSGAEVMVCGPATLIPKYIEQLGVKVETNLINALNWCD
                     VANVLRIQLERQDIKYFPSLREYIMQYGITLDILKKLQKKIVIMHPGPINRGVEITTE
                     VADSDQSIILDQVENGVAVRMAVLYLLAGQPVG"
     gene            complement(2937..3506)
                     /gene="pyrR"
                     /locus_tag="PK134_04655"
     CDS             complement(2937..3506)
                     /gene="pyrR"
                     /locus_tag="PK134_04655"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRB38171.1"
                     /translation="MTHLILAGKQLDITIERLCHQLIENHGNFKDTCIIGLQSKGVPL
                     SKRICKRLSEFLPESSILHGALDITFFRDDYRRSEKLLVPSRTEIDFIVEGKKVVLVD
                     DVLFTGRTVRAAMDALMAFGRPAKVELLVLIDRKFSRQFPIEPDYTGQAIDTTITDKV
                     QVEWKESGNKDGVWLVSEIKSKTNGKAKR"
     gene            complement(3622..4461)
                     /locus_tag="PK134_04660"
     CDS             complement(3622..4461)
                     /locus_tag="PK134_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HRB38172.1"
                     /translation="MTNFIPVRVAVLDLYNGEPNEGMRSIRSILERFSKFTVCSFFDV
                     RQKNEVPDLSFDIYISSGGPGCPLPAGEEWEEPFYALWDKLIEHNADTENKKKYAFLI
                     CHSFQMMAHHLGFGVVSERHSPSFGIFPIHKTDDGNNDRIIGRLPEPFYAVDSREWQV
                     TTPDLDKIVHSGGKLLCLEKMRPHVPYERAIMGLRFSDEILGVQFHPEADAEGMYRYF
                     DRPDKKEKIMELYGSEKYYDMIESLSDPNKIELTHHAIIPCFLADAIEHLYPQLKMQK
                     ATV"
     gene            complement(4458..>5314)
                     /locus_tag="PK134_04665"
     CDS             complement(4458..>5314)
                     /locus_tag="PK134_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRB38173.1"
                     /translation="THPFSRWQDQLITDHPRYEEIIREMQDAARSNLIFGLHVHVGID
                     DREIGIRLMNQARYFLPHIFALSTNSPFWEGRNTGFKSFRTKVFDKFPRTGIPDEFSS
                     AAEYDAYIQLLVKTNCMDNAKKVWWDIRLHPFFNTIEFRICDIPMRIEETITLAAIMQ
                     GIIAKLYLLMQFNLSFRTYSRALINENKWRAARYGIDDKLIDFGKEKEVETKLLIEEI
                     TEFIDDVVGDLGSRKEVDHVFSMMKRGTGADRQLAVFEKTGDLKKVVDYIISETNIDI
                     PVKKEKKK"
     assembly_gap    5315..5342
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5343..5552)
                     /locus_tag="PK134_04670"
     CDS             complement(<5343..5552)
                     /locus_tag="PK134_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HRB38174.1"
                     /translation="MPQFTLGVEEEFQVIDPKTRELRSHMHQIVEGGKVILKEQVKAE
                     MHQSVVEVGTNICKDVSEARKEVTYL"
     gene            complement(5597..>5968)
                     /locus_tag="PK134_04675"
     CDS             complement(5597..>5968)
                     /locus_tag="PK134_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38175.1"
                     /translation="HLRYVVDGPAPEQRILDVVEEYTFKLCNALGYDINTVEFAIRDG
                     VPYAIDFCNPAPDADRHSVGDENFNWVIEAVADMCIEKALSHREGQDNLTWGAFVKHA
                     AQGQIVNKQNMDALMESYTVK"
BASE COUNT         1766 a         1255 c         1056 g         1863 t
ORIGIN      
        1 taaaagtttg tcaacaccgg ctttttttgc aattgttgca gcttgcagac ttgttgaatg
       61 aaaagttgtt tttgcacgtt ctgccaagtc attcataaat gtagcttcat gataaagcag
      121 gtttactcct tttatttcat caataattga ttcatcataa caagtgtcgc tacaaaaagc
      181 ataagagcgt ggtgctgttg acgaaattgt caactctgta tttggaataa tttttccttc
      241 gtcatcctca tagtcaaaac catctttcag tctgccataa aaagaaaaag gaatattatg
      301 ttgttgcagt tttgagataa ttaactttct tggctttggt ttttcagaaa ataaatatcc
      361 tgtacatgga atacgatggt tgagaacaat tgatttgatg agcatatcat cgtcctcata
      421 aataattgca ggcgcataat gctgcacagg atgaaaaatt aactgatatc ttaatctggt
      481 ttgcgacaat cgcaattgaa gttctataac atccattaac tcgggctgac catagatgtg
      541 aatttctgtg gtgcgccctt gcagatgcat ggttgaaagc aaacctacaa gacctaaata
      601 atggtcgcca tgcaggtgtg aaataaaaat atgagaaagt ttattgagtt taaatttata
      661 tttcagaaac tgcccttgcg tgccttcgcc acaatcaatc ataaaaaacc gctcccggat
      721 attaagaacc tgagcggtgg gatgaccttg atgtattggc gttgctgaac tgctgcctaa
      781 gatcgttaat tcaaaattca ttacttggaa ataaccatcc ttcttgtaat gttggctgtg
      841 cctgtattaa tagaatacat ataaattcct gacgacaggt tctctgcatt gatagtataa
      901 gtattcacac ctctttttgc agaaatagtt tcgctgaaaa tatttttacc aagtatattg
      961 taaattttaa aatcaagttt tgtgttaaca ggtgaattaa aatatacaac agatttgttt
     1021 ttaaaaggat ttggcaggtt ttgcgacatg ctcaatttat cttttggcag catttctatt
     1081 actcctgaat tggtgtcaat agtaatagta taatatgtta ccgacatggg ctggccgtaa
     1141 gatatatttc caaggttagt aatttttcca cgagctatga tatcaacagt tatcggatac
     1201 acacctgctg taacaggcgt acctgaaagt aacaaacatc cgttgctgct tgccaaaaac
     1261 tgacacgttc ctgggttgca tgcataagtt aatccgggag gtaatcccga aacacctaaa
     1321 atggtaattg aatccaattc aaaatttgca ttgaatggca caccaccaat attgattgtg
     1381 gcagtagtat caaaaaatgt tcttacctga ataacctgat tgtaggctac acccaccatg
     1441 ccattgctca gacctgtagc actgtcggga taaaccccgg gatcaattat tgttgggtca
     1501 ggagtacact gcgaaactgc aatgcctgac atagagataa acatcaatga gaagagtagc
     1561 ttttttatca tttttcagaa ttttaaattt aacttacaga agcggcttta ttgattagag
     1621 ccaatgcttc atcaattttt ggtacaatgg ttaatacaga atcaagttga gaaatgttga
     1681 ttagtttttt tacaggatcg ctgatttcag taacaatgaa attaccattg gcattgcggc
     1741 aaattctatt gcctactaaa atagcactca gtcctgaaga gtcacaatac tgtgtgtcgg
     1801 aaagatcaag cacaatatga ataaagccct gactgttgag tatcaacaat tccgacttca
     1861 gttctgcaga tataagtgta ttaagctttt gttcgtgaag ctttagcact gcgtattttt
     1921 ctttcttgtc taaggtataa tgcatgagta ttattataaa attggtgcaa acaaatataa
     1981 aaaagatatt ttaattaaaa acaaattggt ctttcgactt ataaaatcaa cctaccggct
     2041 gaccggctaa tagataaagt actgccatgc gcacggccac gccattttcg acctgatcta
     2101 aaattataga ctgatcggaa tccgcaactt cagtagtaat ttcaacccca cggttgattg
     2161 gtccgggatg catgatgaca atctttttct gtaacttttt cagtatgtcg agggtgatgc
     2221 cgtattgcat aatatactct cttaaagagg gaaaatattt tatatcctgc ctttcaagct
     2281 gaatgcgcaa tacattagct acatcgcacc agttcaatgc attaatcaag ttggtttcaa
     2341 ctttcacacc tagctgttca atatattttg gtatcaatgt agccgggccg caaaccatca
     2401 cttctgcacc cgatttttgc agacaataaa tatttgacaa tgccactctt gaatgtaaaa
     2461 tatcacctac tataactact tttttgcctt ttagtgttcc taatttttcg cgtatagaaa
     2521 atgcatcgag tagtgcctgt gtagggtgtt catgtgtacc atcgcctgca ttaacaataa
     2581 cagaatttac tttctgcgat aaaaacaaag ctgctccggg ttttggatga cgcatcacta
     2641 ccaaatcaac cttcatggca agaatattat ttaccgtatc aattaatgtt tcgcctttgc
     2701 ttaccgatga tgaagaagct gtaaaattta gtatatcagc agagagtctt ttctctgcca
     2761 actcgaatga aaggcgtgtg cgtgtagaat tttcaaaaaa cagattcact actgtaacat
     2821 ctcgcatgga aggcactttc tttatcgggc gattgatgac ttctttaaaa gtttgtgccg
     2881 tatcaagaat aaggttgata tcttcaggtt tcagatcttt gattccgata agatgcttaa
     2941 cgcttagctt tcccattcgt ttttgattta atttcagaaa ctaaccacac gccatcttta
     3001 ttgccgctct ccttccactc tacctgcact ttatcagtaa tggttgtatc aattgcctgc
     3061 cctgtataat caggttcaat cggaaactgt ctgctgaatt ttctgtcaat taaaaccagt
     3121 agctctacct tggcaggtcg cccaaaggcc atgagtgcat ccattgctgc tcttactgtt
     3181 cgtccggtaa acaatacatc gtcaaccaaa acaaccttct tgccctcaac aataaaatct
     3241 atctctgtgc gcgatggaac aagcagcttt tcacttctgc gataatcgtc tctgaaaaat
     3301 gtaatatcca atgcaccatg aagaatactg ctttccggca aaaactccga aagtcttttg
     3361 caaatacgct tagataatgg aactcccttc gactgaagtc ctatgataca ggtatcttta
     3421 aaatttccgt ggttttctat caactgatga caaaggcgct ctatagtaat atctaactgt
     3481 ttgcccgcaa gaattaaatg tgtcatcgtg tggcaaaaat agcatttgaa actaattgta
     3541 aaaaatcaaa ttaccttgcc actcaaaaat ttacagtaaa attttgcaat ggagtaaatt
     3601 acaataaagc tatgccgatt attaaactgt tgccttttgc atttttaatt gcggatacaa
     3661 atgctctatg gcatctgcca aaaaacatgg tataatggca tggtgtgtca gctcaatttt
     3721 attggggtcg ctaagcgatt caatcatatc ataatatttt tcgctgccat aaagctccat
     3781 aattttttct ttcttatcag ggcgatcaaa atagcgatac ataccttctg catcggcttc
     3841 cggatgaaac tgcacgccca atatttcatc actaaatcgc aatcccatta ttgcacgctc
     3901 atacggaaca tgtggtctca ttttctctaa gcaaagaagt ttgccaccac tgtgcacaat
     3961 tttatcaaga tcgggagttg tcacctgcca ttctcttgag tcaacagcat aaaacggttc
     4021 gggaaggcga ccaataattc tgtcattatt tccatcatcg gttttatgta taggaaaaat
     4081 tccgaacgaa ggcgaatgtc tttcgctcac tacgccaaag cctaaatgat gtgccatcat
     4141 ctgaaatgag tgacatatca gaaaagcata tttcttctta ttttcagtat cagcattgtg
     4201 ctctatgagt ttatcccaca gtgcataaaa aggctcttcc cattcctcac ctgcaggcaa
     4261 agggcaaccg ggcccgcctg acgaaatata aatatcgaaa ctcaggtcag gcacttcatt
     4321 tttttgtctt acatcaaaaa agctacacac tgtaaatttg ctgaatcgct caagaataga
     4381 gcggatgctg cgcatgcctt cattgggttc accattgtat aagtcgagta ctgcaacgcg
     4441 cacaggaata aaattcgtca tttctttttt tcctttttta cgggaatatc aatatttgtt
     4501 tcactaatta tgtaatctac cactttttta aggtctccgg ttttctcaaa aactgccaac
     4561 tgcctgtcgg caccggtgcc tcttttcatc atactaaaaa catgatctac ctctttgcgc
     4621 gagcctaaat cgccaacaac atcatcaata aactctgtaa tttcttcaat cagtagcttt
     4681 gtttctacct ccttctcttt accaaaatca atcagcttat catcaatacc ataacgggct
     4741 gcacgccatt tattttcatt gattaatgct ctggaatagg ttctgaaact caggttaaac
     4801 tgcatcagca aatacaattt ggcaataatt ccctgcataa tagctgccag cgtaatagtt
     4861 tcttcaatgc gcattggaat atcacaaatc ctgaattcaa tagtattgaa aaacggatgc
     4921 agtcttatat cccaccaaac cttcttagca ttgtccatgc agttggtttt tacaagcaat
     4981 tgaatgtagg catcatattc ggcagcactc gaaaactcat cgggaatacc cgttctcgga
     5041 aatttatcaa acaccttagt tctaaaagac ttaaaacctg tgtttcttcc ttcccaaaaa
     5101 ggagagttgg tagagagtgc aaaaatgtga ggcaaaaaat aacgtgcctg attcatcagc
     5161 cttataccta tttctctgtc gtcaatgcca acatgcacat gcaaaccaaa aatcagattg
     5221 cttcgtgcag catcctgcat ttcgcgtata atttcttcgt agcgtggatg gtctgtaatc
     5281 aactgatctt gccagcgcga aaacggatgt gtccnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nncagatagg taacttcttt gcgggcttcc gaaacatctt tgcaaatgtt ggtacccact
     5401 tccactacac tctgatgcat ttcggccttt acctgctcct tcaaaatcac tttgccgcct
     5461 tctacaatct gatgcatgtg cgagcgcagc tctctcgtct tagggtctat aacctgaaac
     5521 tcctcttcta caccaagtgt aaattgtggc agccctaatt ttttattaat gtatgacata
     5581 ttctttttta cgctggttat ttcaccgtgt aagactccat cagtgcatcc atattctgct
     5641 tgttcacaat ctgtccttgt gcagcatgtt taacaaaagc tccccatgtc aggttatcct
     5701 gtccttcgcg gtgcgaaagt gctttttcaa tacacatatc ggcaacggct tctatcaccc
     5761 aattaaaatt ctcatcgcca acactgtggc ggtcggcatc gggggccgga ttgcagaaat
     5821 ctatggcata aggcacacca tcacgaatgg caaactcaac cgtattgatg tcatagccca
     5881 atgcattgca aagcttaaaa gtatattctt caacaacatc aagaatacgc tgctcgggtg
     5941 caggcccgtc aaccacataa cgcaggtg
//
LOCUS       DAORQT010000097         5916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2217_length_5916_cov_7.696809, whole genome shotgun sequence.
ACCESSION   DAORQT010000097 DAORQT010000000
VERSION     DAORQT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_5916_cov_7.696809"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK134_04680"
     CDS             <1..623
                     /locus_tag="PK134_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38176.1"
                     /translation="FSMVITDVQMPGMDGYQLAGHIRSKLPDPINTIPILALTAYESS
                     AEKEKAKAAGMSGFLTKPYMPEQLFDLITQHLPLKNDQSKDAVEYESTYNVNEIYDNL
                     LALMAGDKKETASLIKMLQVQFPELLSEMLQAIEDKDMKKLYKAAHKIKSSLKIFNNN
                     SVGLLIDQVERKAKDNEYSFELSVKVSELIKQVNVIIGSLDQLIKK"
     gene            complement(634..1185)
                     /locus_tag="PK134_04685"
     CDS             complement(634..1185)
                     /locus_tag="PK134_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRB38177.1"
                     /translation="MKQILMNEKLTFRVVAIISVVVLGMVMLLSQKVLPRPETPPAFT
                     KYLPGLNAFVNGTCFVLLLISFYFIKKKRIDIHKKINLLTFLLSAVFIVSYFLYHYLA
                     EETKYPVDHPLRGLYLFILASHIILAAIVMPLVLLSFYYGLKMDVRKHRKLTRFSFPI
                     WLYVTLTGVIVYLMISPYYSFNQ"
     gene            complement(1187..1780)
                     /locus_tag="PK134_04690"
     CDS             complement(1187..1780)
                     /locus_tag="PK134_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRB38178.1"
                     /translation="MPLFFFILMKEGKHNFRYLEYYGPKHLATNGVDTIYHSISDFSL
                     KNTEGNTVSPSNTEPSYWVFQFFNASCQEPCKEVFTNMYDVARDFVTAPKVKFVSITT
                     QPFNDSLPVMKHVMKMNSIHPPQWQLCTGDSLGIDSLMRTSFFLKGNTNAALNSVFLV
                     DSKLHLRGIYDGADLLEMKRLKDDIKILSYETRQKSK"
     gene            complement(1834..2199)
                     /locus_tag="PK134_04695"
     CDS             complement(1834..2199)
                     /locus_tag="PK134_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRB38179.1"
                     /translation="MSHNEHNEHKPHGYYTGDIHNQFVEEHYDPTAKKIIWRTFWILL
                     VATIVEVAIAFIPMHKDLLQWIFVALTIFKSAYIVGVFMHLGKEKKSLQYTILLPFFL
                     IVYMIAICLIEGVYLFHIG"
     gene            complement(2224..3039)
                     /locus_tag="PK134_04700"
     CDS             complement(2224..3039)
                     /locus_tag="PK134_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRB38180.1"
                     /translation="MSTATEAKSKSIWGGGISPFNVSWGKMFMWFFLVSDAFTFSTLL
                     VAYGTMRHRFTDIWPAPGDVFTHFPFIHEHVPLAYVGLMTFILIMSSVTMVLAVDAGH
                     QRNHKSVAMWMGLTIVGGLMFLGSQAWEWYHFIHGSKEGTYILADGRFARTDDYEPGK
                     LFFWHNHQKTVVEGAEAEKLKASAVRNIKGANMTENEYGNTPLFANFFFFITGFHGFH
                     VFSGVVINIIIFINVLNGVYKRRGHYEQVEKSGLYWHFVDLVWVFVFTFFYLV"
     gene            complement(3173..3745)
                     /locus_tag="PK134_04705"
     CDS             complement(3173..3745)
                     /locus_tag="PK134_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRB38181.1"
                     /translation="MQTVTTEQDNYTGIPTAKILLWIAMASMVMLFAGLTSAYIVRKN
                     EGNWIEFSLPKMFTISTITIIISSFIIQYAIYAIKRNLLNQVKIAVITTLGLGIAFVF
                     MQFYAWTVMVNNGIHLVGNPSGSFIYVLSGMHILHLPGGIISMIVISVKSILERYSPK
                     NYLGLQLGAIFWHFLDILWVYLFVFVTFIA"
     gene            complement(3751..4638)
                     /gene="cyoE"
                     /locus_tag="PK134_04710"
     CDS             complement(3751..4638)
                     /gene="cyoE"
                     /locus_tag="PK134_04710"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRB38182.1"
                     /translation="MLKTTMVVAPRTFTARLVDYGQLFKLRLASLVVLSAALAFLIGS
                     TTIDYTKLWLLVLGGFLVTGSSNALNQIYERRTDKIMKRTRMRPLPDSRMSLTESLVV
                     TIAAGLLGLFILTYYINLYSGILGLIALVSYAFAYTPLKGVTPLSVFVGAFPGAIPTL
                     LGWVAATGQFSYQGWILFSIQFMWQFPHFWAIAWKLYDDYMSAGFQLLPSGGKNSASA
                     FQTTIYTFTLIPIGLLPYYSGMSGIVSAVVITLAGILFFMQSVYLLKTCSDIAAKRLM
                     FGSFIYLPVVLIAILLDKI"
     gene            complement(4786..4989)
                     /locus_tag="PK134_04715"
     CDS             complement(4786..4989)
                     /locus_tag="PK134_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38183.1"
                     /translation="MLNSIWHGIAAFFEFIFSLAKPIGKSVDIVFMVLIAIGLLYWLI
                     YEQRVMGGKTRNYLADKVKEKNK"
     gene            5061..>5916
                     /locus_tag="PK134_04720"
     CDS             5061..>5916
                     /locus_tag="PK134_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38184.1"
                     /translation="MLIVRLFKSSQTPPLFLVPVITLALVLFNIYTQGVVVIRDMNHG
                     VLYALLLDSNIAGIKWAVPSLIFVLITIQAFLFNALINRFEVLYKSSNIPVIIFIILY
                     GLIPGFLALSPFLLVNTLMPVALWKIFTLYKSERPVTRAFDTGLVFSLMILCYLPMAL
                     LVVFMYLCIAILRPFNWREWVASALGLLTPAILLFTILFVADKLHLRNTEVNTDSWLP
                     VIRLQSLSWRFILPALVMAILLFLSFLKLYANYSKNVIRMRKFQLCFMLLLIFLLLII
                     LIPVQQSAS"
BASE COUNT         1991 a         1077 c         1061 g         1787 t
ORIGIN      
        1 attttagtat ggtaattaca gacgttcaga tgcccggaat ggatggctac caactggcag
       61 ggcatatacg aagtaaacta cctgatccca taaatacaat acctatcctt gcattgacgg
      121 cttatgaatc gagtgcagag aaagagaagg caaaggctgc aggtatgtca ggttttttaa
      181 cgaaacccta catgcctgag caactgtttg atctcataac acaacatctt ccattgaaaa
      241 atgatcaatc aaaagatgca gttgaatatg aatcaactta taatgtcaat gaaatttatg
      301 acaacctttt ggctttaatg gcaggagata aaaaagaaac agcttcattg ataaaaatgc
      361 ttcaggtaca atttcctgaa ttactcagtg aaatgttgca ggctattgaa gacaaggata
      421 tgaagaaact gtataaggct gcgcataaga taaaatctag tctgaaaata ttcaacaaca
      481 attctgttgg gttattaata gatcaggttg agcgcaaggc taaagacaat gaatattctt
      541 ttgaattgtc agttaaagtg tctgagctta ttaagcaggt gaatgtaatt attggttcac
      601 tagaccaact aataaaaaag taaacagaaa attttattgg ttaaacgaat aatagggtga
      661 tatcatcaga taaacaatca cacctgttaa tgtaacatac aaccagatag ggaaagaaaa
      721 acgtgtaagc ttacgatgtt tacgcacatc catttttaaa ccataataaa acgataacag
      781 taccaatggc atcactatgg cagcaagaat aatgtgcgat gctaaaatga acagataaag
      841 tccgcgcaac ggatggtcaa cgggatattt ggtttcttct gctaaataat gatatagaaa
      901 ataagataca ataaaaacag cactcaataa aaatgtaagc aggttaattt ttttatgaat
      961 gtctatgcgt ttctttttga taaaataaaa tgaaattagc aacaggacaa aacaagttcc
     1021 gtttacaaac gcatttaaac cgggaaggta cttcgtaaat gccggaggcg tttcaggacg
     1081 tggaaggact ttctggctta gtagcataac cattcctaat acaacaactg aaataattgc
     1141 tactactctg aaagtaagct tttcattcat taatatctgt ttcatcttac tttgattttt
     1201 gtcttgtttc gtaactaaga attttaatat catctttcaa tcgtttcatc tctaataaat
     1261 cggcaccgtc atagatgccg cgaagatgca acttactgtc aaccaaaaat acagagttaa
     1321 gtgctgcatt ggtgtttcct ttcaggaaaa atgatgtgcg cattaaagaa tctattccca
     1381 atgaatcgcc tgtacataac tgccattgcg gaggatgaat gctgttcatt ttcataacat
     1441 gtttcattac aggtaaactg tcgttaaatg gctgtgtcgt gatggaaaca aatttcactt
     1501 taggtgcagt tacaaaatcg cgtgccacat catacatatt ggtaaaaact tctttgcacg
     1561 gttcctgaca agaagcatta aaaaactgaa atacccagta agagggttcg gtgttgcttg
     1621 gtgaaacagt atttccttct gtatttttta acgagaaatc agatatggaa tgataaatgg
     1681 tgtcaactcc gtttgtagcc agatgctttg ggccataata ttcaagatat cggaaattat
     1741 gtttcccttc tttcatcaga ataaaaaaaa ataaaggcat cagaagtatt aaaaaaaccg
     1801 ctgctgcctt aaactttttc aagataaaaa agcttatcct atatggaaca gatatacacc
     1861 ttcaataaga caaattgcaa tcatgtaaac aatcagaaaa aatggtaaga gaatggtgta
     1921 ctgaagactc ttcttttctt ttccaaggtg cataaaaaca cctactatat atgctgactt
     1981 aaaaattgtt aatgcaacaa atatccattg caacaaatct ttatgcattg gaataaaggc
     2041 tattgctact tcaacaattg ttgcaacaag caatatccag aatgtacgcc agataatttt
     2101 tttagccgta gggtcgtaat gctcttctac aaactgattg tgtatatcac cggtgtaata
     2161 accgtgtggc ttatgctcat tatgttcgtt atgactcatg attctatttt tcttaaagat
     2221 tagttataca agatagaaga aggtgaatac gaatacccaa accaaatcta caaagtgcca
     2281 ataaagcccc gatttttcaa cctgctcata atgaccgcgc cttttgtaaa cgccattgag
     2341 cacattaata aaaattatta tgttgatgac tacaccactg aaaacgtgaa aaccgtgaaa
     2401 ccctgtaata aagaaaaaga agttggcaaa taagggggtg ttgccatatt cattctcagt
     2461 catgtttgca cctttaatgt tgcgcactgc agatgcttta agtttttctg cttcggcacc
     2521 ttcaacaaca gttttctgat ggttgtgcca gaagaataat tttccgggtt cataatcgtc
     2581 tgtgcgtgca aaacgaccat cagcaagaat ataagttcct tcttttgaac catgaataaa
     2641 gtgataccac tcccatgcct gactgcctaa gaacatcaaa ccgccaacaa tggttaatcc
     2701 catccacatg gcaacacttt tatggttgcg ctgatgtcct gcatctactg caagtaccat
     2761 tgttaccgaa ctcatgataa gaataaaggt cattaaacct acataagcta aaggtacatg
     2821 ttcgtgtata aaagggaagt gcgtaaacac atcacccggt gcaggccata tatctgtaaa
     2881 acgatggcgc atggtaccat aagccaccag gagggttgag aatgtaaaag catcagatac
     2941 caggaaaaac cacataaaca tttttcccca gcttacattg aatggtgaaa ttccaccacc
     3001 ccagatggat tttgattttg cttcggttgc tgttgacata ttaagttagg agtcttgaaa
     3061 ttaatttaac aggatgcaaa tatttaattt tttttccaat tcatcaattt attgtaaaat
     3121 ttttggccat ttaaccataa ttcattgaca gattggagca aaagatagcc tttcaggcta
     3181 tgaaggtcac aaatacgaag agataaaccc ataggatatc aagaaaatgc cagaaaatgg
     3241 cacctaactg aagtcctaaa tagtttttgg gagaatatct ttcaagaata gattttactg
     3301 aaataacaat catggaaatt attcctcctg gaaggtgcag aatgtgcatt cctgacaata
     3361 catatataaa agaacctgat ggattaccta ctaaatggat gccgttgttt accataactg
     3421 tccaggcata aaactgcata aagacaaaag caatacccag cccgagagtg gttatgactg
     3481 caatttttac ctgattcagc agatttcttt taatagcata aatagcatat tgaattataa
     3541 aactgctaat aattatggta atagtgctga tggtaaacat ctttggtaaa ctaaactcta
     3601 tccagttgcc ttcgttttta cgaacgatat aggcacttgt caaaccggca aatagcataa
     3661 ccatactggc catggctatc cacaataata ttttagctgt gggaatgcct gtgtaattat
     3721 cctgttctgt ggtgactgtt tgcattgtaa ttaaatttta tcaagtaata tggcaattaa
     3781 tacaacgggg agatatataa atgaaccgaa catcagcctt tttgctgcaa tgtccgaaca
     3841 tgtttttaat aaataaacac tttgcataaa aaataaaata cctgcaaggg taatgactac
     3901 tgcagataca attccactca tgccactgta gtatggcagc agaccgattg gtataagtgt
     3961 aaaagtatat attgttgttt gaaatgcaga agcagaattt ttaccacccg aaggcagcaa
     4021 ttgaaagcct gcactcatat agtcgtcata aagtttccag gcaatagccc aaaagtgtgg
     4081 aaactgccac atgaattgaa tagaaaaaag tatccatccc tgataactga actgaccggt
     4141 tgcggctacc caaccaagca gggtaggaat agcacccgga aacgctccaa caaaaactga
     4201 taatggtgta acacctttta atggtgtgta agcaaaagca taacttacta aagcaatcaa
     4261 gccaagtatt ccgctgtaaa gattaatgta gtaggtgagg ataaacaatc ctaataaacc
     4321 tgctgcgata gttacaacaa gcgattctgt aagcgacatt ctgctgtcag gaagcggacg
     4381 cattctggtg cgcttcataa ttttatcggt gcgtctttcg taaatctgat tcaatgcatt
     4441 tgaagaaccc gtaactaaaa agccacctaa aacaagaagc cacaacttag tgtaatctat
     4501 cgttgttgaa cctataagaa aagctaatgc agcagagaga actactaacg aagccagcct
     4561 tagtttaaac aactgtccat agtcaacaag tctggcagta aaggtacgtg gtgcaacaac
     4621 cattgttgtt ttcaacattt aagcaaaatg aaaaaatttc ggaatgcaaa ggtataaaat
     4681 tgatggtaag gaagcttgaa aatgctaatt acttactgat aaacataaaa aaaggcagcc
     4741 aaaggctgcc ttaaaattct tttctatgaa gtaaatgtca aatggttatt tattcttttc
     4801 cttcacttta tcagcaagat agttgcgggt ttttccaccc ataactcttt gttcgtagat
     4861 gagccaatat aacaatccaa tggcaattaa taccataaaa actatatcta cagattttcc
     4921 aatgggtttt gccaagctga aaataaattc aaaaaatgct gctataccgt gccagattga
     4981 gtttaacata ttcaataatt ttttgcaaaa ataataaaat tcaaatacag atattcgcag
     5041 ccacaaagtt gctgaaaaaa atgttgatcg tcaggttgtt taaatcttcg caaacaccac
     5101 cactgttttt agtgccggtg ataacactgg cattagtact attcaacatt tatactcaag
     5161 gggttgtagt tatcagagac atgaatcatg gtgtgttgta tgccttatta cttgacagta
     5221 acattgcagg aataaaatgg gctgtacctt cattgatctt tgtactcatt accattcagg
     5281 cttttttatt taatgcactc atcaaccggt ttgaggtttt atacaaaagc agcaacattc
     5341 ctgtcattat attcatcatc ctctatggac ttatacccgg tttcttggca cttagtcctt
     5401 ttcttttagt aaacacattg atgcctgttg cgctatggaa aatttttaca ttatataaat
     5461 cagagcgtcc tgttacccgt gcatttgata caggtcttgt tttttcgctg atgatattgt
     5521 gttatctacc aatggcattg ttggtagtct ttatgtactt atgtattgcc atactacgtc
     5581 cttttaactg gcgcgagtgg gttgcttctg cattaggact tttaactcct gcaatactgt
     5641 tgttcacaat tctttttgtg gcagacaaac tgcatttgcg aaatacagaa gtaaatacag
     5701 attcatggct tcctgttatc agactgcaat cgctttcatg gcgttttata cttcctgcat
     5761 tagtaatggc aatccttttg tttttatcgt ttctgaaact atatgctaat tacagtaaga
     5821 atgttatccg catgcggaaa tttcagttat gctttatgtt attgcttatt tttctgttgc
     5881 tcataatatt gattcctgta caacaaagtg catcac
//
LOCUS       DAORQT010000098         5882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2239_length_5882_cov_9.276815, whole genome shotgun sequence.
ACCESSION   DAORQT010000098 DAORQT010000000
VERSION     DAORQT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_5882_cov_9.276815"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5424
                     /locus_tag="PK134_04725"
     CDS             <1..5424
                     /locus_tag="PK134_04725"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38185.1"
                     /translation="QYAVVGNSEDCMNNISVYSVSGTPLPATYIWNVTGGNVVSTGTG
                     TVSIDWSSVGSGSVGVTLYDVSGAVIGTPSLTVTVNPLPQPVLTTDFTNNCVIYKEHE
                     GQVIPTVVNDTCWHVCENGIVHYFANPVNVGSSFNWNVTGAQSFVAISNNEIEVIWGS
                     AGNGTVSVTETDGNGCVNTVEKCIVIIESPQAIITAFPGDVDCRLGGVITVCLEQNAL
                     FFGNANLGNSGSPIVSWLWDFGDGTVSTHRDEVHSWQTPGIYQTTLTVTNACGCTGIC
                     YVSVEVDPVNGVNIECPTPVCQGDTGKYYTDAVNCSAYNWVVTGGNILSPMPYGSTIQ
                     VQWTGGNGNGILCLDNTNGTCGNYCPSETCVEIPIISSTSSIDGPSLVCIGSQTKYNV
                     PAMTGCVYNWQITGSSGTIVSGQGTNEIMVDWTSAGTSTISVNYNNALLNCGGTANLT
                     VETKQPFSINGPTKGCPGVDVVLTANNPVNVLLDWTITDASQNIIYVGSGTGVPDFTI
                     TGWSYPSGTYIISALDNSNSYCNYTASITIEIKDPPPSPAMPVGDNPICPGTVHLYSG
                     TTMNAGYYLEWSVTGGTPTIGTGNELSIGWINTTPPHNYSISLVQVESSTGCKSLPTV
                     LPIQERLYINPVITGNFTPCINKQVTYQISNAVPNGNYIYDQLEWVIVPATAGSVISG
                     QGTTTVTVQWNNQPSPVNVYDTYLQVKTSLCGSPPFPYPNPLFDPPNISLGGAPALAI
                     TAIPNPACENTPITFTATPALPGNYTWSFGDGSSGNSPLNAVHTYSLTQGASLTTFDV
                     TLTVDDGGCQSYASTKIDVNPAPLYSLTLSATTFCPPAVTNITASLVQQSGSFNYSWS
                     NNASNNSNVNTLNYLDAQPFDVTITNTITGCARTESFSISTCSPNPPLCNPTPLLYIG
                     FTTSPASDCFTVNFVGSINPTPASYLWDFGDNTSDNTTLTPSHTYSTAGQFSACLYAF
                     DNTGLQYCDNYCLPVSVPLFPNFITSLHCSSTPGSYDVEFIDLSSFTIPITTWQWFSG
                     TTQFSTSNHPTITLAAGTYNIKLKIGNGTDFCERTLTINIPAPPSPAFTVAGGPFCEG
                     QTVVQFSASNTNVHHALWDFGDQATYNPQNPAATINAQRVFESGLSIYSVNLTVTDNY
                     GCTYSSTQQVTVNDNNLGGSISPVLISFCPGGNGSLALDYITIPGGPYTQPTSWLWTG
                     GVTTPTLTVNQTGQYFVTVSDNNGCSYSPASPGQAAVVNVPKPFIWGDLTLCEGDNIN
                     LDGNQGSAVQYNWTIVDPNNVTNTFSQSSIHIAGAVAGTYHVTLDITANGCTRSTSVD
                     VLVNPLPPLPIIQSNVPQACEGQPIDLTVINAGSYWVNWSNGASGPVITVYTAGAYQA
                     TLTDANGCTNHATFEVHENPFSGFMMTGCVQYCDTVPVTVFGSGSLSFNKWIWYVDGV
                     AQASGQQSPVQDLNMGLLSPGVYVVTLQLVVGYPDGTVCEMMSDALEITIIPCPCRLY
                     PQGKIYCMNEVNGDVQLLNNYHFEIVTNYGCSANPNVMVSSPDGGVSMLPNSNPPQLL
                     TGILSTYSAYPDNVCFDINITDSNKPMCSCNYRYCMKLPKCGVPTGCSTEVKIDNVQC
                     IGHDANGNRIYSFTLTLLNSPQLFSMFTAQGGVLGAMPATVGPGTTIINSSITVNNNA
                     SHFCITLHGYNFTTYTACKTTGCSGNLPPCGIPPSRNGEKDTTTPLRVSYQNDANDAV
                     QNTSLSLMPNPAKNKVTLHYSTRSGGIITIASAVGQIIYNENVATAGMSELSTQQWLP
                     GIYFVTLQSNAGDCLVQKLVIIK"
     gene            5433..>5882
                     /locus_tag="PK134_04730"
     CDS             5433..>5882
                     /locus_tag="PK134_04730"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRB38186.1"
                     /translation="MKKTIKLLLIFLMLFTSVAAQTPSWHWLARVGGYSGNGNGAGGP
                     DEWVKDVKTDAQGNVYVCGRVCYGANFNGDTMTTYQGYFSLFLAKLNCAGDLVWVKTA
                     GNTSNLYNTEAYGLALDNYGNIYLTGYLGAHPLSPCQFFDTLITEETY"
BASE COUNT         1728 a         1260 c         1229 g         1665 t
ORIGIN      
        1 caatacgctg ttgttggcaa ctcagaagac tgcatgaata acatatcggt ttattctgtc
       61 agcggaacac cacttccggc aacatacatc tggaatgtta caggaggcaa cgttgttagt
      121 acaggcacag gaacagtgag tatagattgg agttctgttg gttctggttc tgtaggtgta
      181 acattgtatg atgtttcggg tgcagtaata ggaacaccat cacttactgt aacagtaaat
      241 ccactaccac aacctgtgct tactacagat ttcaccaaca actgtgttat ctataaagag
      301 catgaagggc aggtaatacc aacagtggta aatgatacct gctggcatgt ttgtgaaaac
      361 ggcatagtac attacttcgc aaatcctgtt aacgttggct catcatttaa ttggaatgta
      421 acaggtgcgc aaagttttgt agctatttca aataatgaga tagaagttat atggggaagt
      481 gccggcaacg gaactgtttc tgtaacagaa actgatggca atggttgtgt aaatacggtt
      541 gaaaagtgta ttgtcattat tgaatcgcca caggcaataa ttactgcatt tccgggtgat
      601 gtggattgtc gtttaggtgg cgtaattact gtttgtcttg agcaaaatgc attatttttc
      661 ggaaatgcta accttggaaa ttcaggttca ccaattgtaa gttggttgtg ggattttggc
      721 gatggaacag tttctacaca tagagacgaa gtacattcgt ggcaaacgcc tggaatctat
      781 cagacaacac tcaccgtaac caatgcatgt ggttgtacag gcatttgtta tgtatctgta
      841 gaggttgacc ctgtaaatgg tgtgaatata gaatgtccaa ctcctgtatg ccagggcgat
      901 acaggtaaat attataccga tgcagtgaat tgcagtgcat ataattgggt agttaccgga
      961 ggaaacattc tttcaccaat gccttatggc agcaccatac aggtacagtg gacgggtggc
     1021 aatggcaatg gcattttatg tctggataat actaacggta cctgtggtaa ctactgtcca
     1081 agtgaaacat gtgttgaaat tcctattata tcttcaacaa gtagtattga tggcccctca
     1141 ttggtttgta taggctctca gacaaaatac aatgtgcctg ctatgacggg ttgtgtttat
     1201 aactggcaga taacaggcag cagcggcact attgtttcag ggcaaggcac caatgaaata
     1261 atggtagatt ggaccagtgc cggaacttca acaataagtg taaattataa caatgcctta
     1321 ttgaattgcg gaggcaccgc caatttaact gttgaaacca agcagccttt tagtattaat
     1381 ggaccaacaa aaggttgccc gggagtagat gttgtactca cagctaataa tcctgtcaat
     1441 gtactattgg attggacaat aacagatgct tctcaaaaca tcatatatgt tggcagtggt
     1501 acaggagttc cggattttac aattacagga tggtcttatc ctagtggcac ctatattata
     1561 tcagcattag acaattcaaa cagttattgt aattacacag caagcataac aatagaaata
     1621 aaagatccgc caccgtcacc tgcaatgcct gttggtgaca acccaatatg tccgggcact
     1681 gtacatttgt acagcggcac aacaatgaat gcaggatatt atttagaatg gagtgtaact
     1741 ggtggcactc caacaatagg cacaggcaat gaattatcta taggctggat taatacaaca
     1801 cctccacata actattccat cagtttggtg caggtagagt catctactgg atgcaaatca
     1861 ttgccaacag tattgccaat acaggaaaga ctttatatta atcctgtcat aacaggaaat
     1921 tttacaccat gtattaataa gcaggtgaca tatcagattt ccaatgctgt gccaaatggc
     1981 aattatatat acgatcaatt agagtgggta attgttcctg ccactgccgg aagtgtaata
     2041 agcgggcaag gcactaccac tgtaactgtg cagtggaata accaaccttc tccggttaat
     2101 gtttatgaca cctatcttca ggtaaaaact tcgctctgtg gctcaccgcc attcccttat
     2161 ccgaatccat tgttcgaccc acccaatatt tcacttggcg gagcacctgc tttagctata
     2221 actgcaatac caaatcctgc ttgtgaaaat accccaataa cttttacagc cactcctgcg
     2281 cttcccggta attatacctg gagttttggt gatggcagca gcggaaacag ccctttaaat
     2341 gcagtgcata cctatagtct gactcaggga gccagcctca caacctttga tgttaccttg
     2401 accgttgatg atggcggatg ccaatcttat gccagcacaa aaattgatgt taaccctgct
     2461 ccattatatt ctttgacact ttcagcaaca acgttctgcc caccggctgt tactaatatt
     2521 acagcctcgc tggtgcaaca atcaggaagt tttaattatt cgtggagtaa taatgcatct
     2581 aataatagca acgttaatac attgaattat ttagatgcac aaccatttga tgtaaccatc
     2641 accaatacga taaccggttg cgccagaaca gaaagttttt caatttcaac ttgctcaccc
     2701 aatccgcctc tgtgcaatcc aacccctctt ctttatatcg gctttaccac ttcaccggca
     2761 tcagattgtt ttacagtaaa ttttgtaggc agcatcaacc ctacacctgc aagttattta
     2821 tgggattttg gtgataatac cagtgataac acaacactta ccccttcaca tacatacagc
     2881 actgccggac aatttagtgc ttgcctatat gccttcgata ataccggact acagtattgc
     2941 gacaattatt gcttaccggt atcggtgccg ctttttccga attttattac ctcgttgcat
     3001 tgcagcagta cacccggaag ttatgacgtt gaatttattg acctttcatc ttttaccatc
     3061 ccaattacca cttggcagtg gttttcaggc accacacagt ttagcacaag caaccaccca
     3121 accattactt tggcagcagg cacctataat attaaactaa aaataggcaa cggcacagat
     3181 ttttgcgaac gaacacttac aataaatatt cctgcaccac cctcacctgc atttaccgtt
     3241 gcaggcgggc cattttgcga agggcaaact gttgtacagt ttagtgcaag caacaccaac
     3301 gtacaccatg ccctttggga ttttggtgat caggctactt ataaccctca aaaccctgct
     3361 gccactatta atgcacagcg ggtgtttgaa tcaggactta gcatttattc agtaaatctt
     3421 acagttaccg acaattacgg ctgcacctac tcaagcacac aacaagtaac ggttaatgat
     3481 aataatttag gtggcagcat aagccctgta ttaatatcat tttgtccggg aggaaatgga
     3541 agtttggcgt tggattatat tacaattcca ggtggtcctt acacacagcc cacctcctgg
     3601 ctatggactg gtggtgtaac tacgcccaca ttaacggtaa atcaaacagg gcagtatttt
     3661 gttacagtga gcgacaataa cggttgcagc tacagtcctg ccagccctgg tcaggctgca
     3721 gttgtaaatg tgccaaagcc ttttatctgg ggcgatctga cactgtgcga aggcgataac
     3781 attaatttag atggcaatca gggtagtgct gtacagtaca actggacaat agttgacccc
     3841 aataatgtta ccaatacctt tagccagtct tccatacata ttgcaggggc tgttgccgga
     3901 acatatcatg taacacttga cattactgcc aatggttgca cccgcagcac ttctgtagat
     3961 gttttagtaa atccattacc gccattgcct attattcaaa gcaatgtgcc gcaagcctgc
     4021 gaagggcagc ccattgatct taccgttatc aatgccggta gttattgggt aaactggagc
     4081 aatggtgcaa gcggccctgt tattactgtt tatactgccg gtgcctatca ggcaacacta
     4141 actgatgcca atggctgcac caatcatgca acctttgaag tgcatgagaa ccccttttca
     4201 ggatttatga tgacaggctg tgtgcaatat tgcgacacag tgcctgtaac agtattcggt
     4261 agtggaagtt tgtcttttaa taaatggata tggtatgttg atggtgttgc gcaagcttca
     4321 ggccagcaaa gccccgtaca agaccttaat atgggattgc tcagcccggg tgtatatgta
     4381 gttacgcttc agttagtagt tggctatcct gatggcactg tgtgcgaaat gatgtcagat
     4441 gcacttgaaa ttaccatcat tccatgtcct tgcagacttt atccgcaagg taaaatttat
     4501 tgcatgaatg aagtgaatgg tgatgttcag ttattgaata attatcattt tgagatagta
     4561 accaactatg gatgctctgc caatcctaat gttatggtgt cttcgccaga tggcggagta
     4621 tctatgctgc cgaattcaaa tccaccgcaa ctacttaccg gaatattatc aacctacagt
     4681 gcctatcctg acaatgtttg ttttgacatt aacataacag actcaaacaa gcccatgtgt
     4741 agctgcaact accgctattg tatgaaattg ccaaaatgcg gagttcccac aggctgtagc
     4801 acagaagtta agatagacaa tgtgcaatgt atcggtcatg atgccaatgg caacagaatc
     4861 tatagtttta cactcacact ccttaattct ccacagctat tctccatgtt tacggcacaa
     4921 ggaggtgttt taggcgctat gcctgcaaca gttggaccgg gcacaaccat catcaacagt
     4981 tctataacag tcaacaataa tgcaagccac ttctgcatca ccttgcatgg ctacaatttt
     5041 actacctaca ctgcttgtaa aactactggt tgctcgggta atttgccccc atgtggtatt
     5101 cctccatcaa gaaacggaga aaaagacaca actacaccac tgagggtaag ctatcaaaat
     5161 gatgctaacg atgctgttca gaatacatcg cttagcctga tgcctaatcc ggctaaaaac
     5221 aaagttacac tacattacag cacccgttcg ggaggcatta tcaccatagc ttctgctgtt
     5281 gggcagataa tatataacga aaatgtggca acagcaggca tgtcagaact ttcaacacag
     5341 caatggctgc ccggtattta ttttgttacc ttgcaaagca atgcaggcga ttgtttggtt
     5401 caaaaattag tcatcatcaa atagcacaaa caatgaaaaa gacaataaaa ttattgctga
     5461 tatttttgat gctgtttaca agtgtggcag cacaaacacc aagttggcat tggctggcac
     5521 gtgtaggcgg ctacagtggt aatggcaatg gtgccggtgg acctgacgaa tgggtaaaag
     5581 atgtaaagac cgatgcacag ggcaatgtat atgtctgcgg cagggtatgc tatggggcta
     5641 actttaatgg cgacaccatg acaacctatc agggttattt tagcttgttt ctggctaaat
     5701 taaactgtgc cggagactta gtttgggtaa aaactgcagg taacacaagc aatttgtata
     5761 atacagaagc ttatggcctt gctttagata attatggcaa tatttattta acaggctacc
     5821 ttggtgcgca tccattaagt ccttgccagt tttttgatac acttataaca gaagaaacat
     5881 ac
//
LOCUS       DAORQT010000099         5812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2280_length_5812_cov_16.321869, whole genome shotgun sequence.
ACCESSION   DAORQT010000099 DAORQT010000000
VERSION     DAORQT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2280_length_5812_cov_16.321869"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..355
                     /locus_tag="PK134_04735"
     CDS             86..355
                     /locus_tag="PK134_04735"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38187.1"
                     /translation="MGTETINFTLAPNPAKDNVTATFDLINETDEATITITDMQGRKV
                     FDKKATAGQYEMQLDVSPLSRASYVAELWINGTRVGRNKLLLIKN"
     gene            410..>1070
                     /locus_tag="PK134_04740"
     CDS             410..>1070
                     /locus_tag="PK134_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38188.1"
                     /translation="MRIIKLFILFCVVTTQTQAQIQFDKRYPLGLASVGRKVVEISGG
                     YICAGFEISDTNTYNNNLIVKSDTNGDTVKTLYIPPPDSTYWNLYNEDPDDIFTDLIV
                     TNDSNLVAVGRSQAYNPTNYYDYDILLVKLNYNLDTLWLKTYSHPTDSAFQPQSIIQT
                     SDGGFLICGKQRSFYNTNWFGFLYKVDSAGNFQWYKSYNNPGPYQAIWGVIELPDHSF
                     MC"
     assembly_gap    1071..1170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1171..1593
                     /locus_tag="PK134_04745"
     CDS             <1171..1593
                     /locus_tag="PK134_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38189.1"
                     /translation="FWDIKTTADGGYIMCGQTGGCNNNGGTCFWLVKTDSLGLLVTGI
                     KEKEIFKTATLTIYPNPATTYCTITAIVPQSNSTGIGKKGASIILFDMRGKQLLEQPL
                     QTGSNTVQLNLTNYSAGTYLAVLVLDGYNAARVKVVKQ"
     gene            1713..4799
                     /locus_tag="PK134_04750"
     CDS             1713..4799
                     /locus_tag="PK134_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRB38190.1"
                     /translation="MKQYTKFNNISGWAVFAIAAWVFLSTIEMTGSFWDCGEFIAAAY
                     KLQIGHPPGAPLFLMLSRIMTLFAGADVKDVPLMVNAFSAIMSAFAVLFTFWTITAIA
                     RKIVFVKNQEPTLAQILTVVASGVVGALAFTFTDSFWFSAVEGEVYATSQFFTCIVVW
                     AVFKWENVADEKHSIRWIILIAYLMGLSIGVHLLNLLCIPALALVYYFRKYPFSWMGV
                     AKTMGIALVILMVVQYGIIAGFVNIGAKIDLWFNQSGSSLWTGFVVYSLIILAALTYG
                     IYYSQKKQKALLNTILLSFAFLLLGYTSYAQIVVRSLANPPMDENNPENAFALLGYLN
                     REQYGDRPLGYGQYYDAKVVGQTEGAMSYTAIDGKYIPTGAKVIPEYDPARCTVFPRM
                     YSREPNHVSAYKEWSGIKGEQKPEFSNNLKYFFSYQMNHMFWRYFMWNFVGRQNDIQG
                     HGSILKGNWISGIPFIDSMFLGPQDKLPDSMKNNKARNTFYFLPLLLGLAGLVYHYKK
                     DMNNANVVMLLFIMTGLAIVIYLNQTPYQPRERDYAYAGAFYAFCIWIGLGVAAIADA
                     LKKRMPETTAAMLATLLCLVVPTVMAKEGWDDHNRSYRYTSRDFAYNYLNSCAPNAVI
                     FTNGDNDTFPLWYIQDVEGVRTDVRVINLSLLNTDWYIDQIKRKAYESDPVPFTLNTK
                     QYVQGTRDYIPFYDRSVQGYSDLKDIIEFITSDNPEAKVRSEGGDEFNYLPTKKFRIK
                     VDKQAVIKNNVVEAKDTSKIVDYIEFEISKNYIMKADLMILDLIAHNDWTRPIYFAVT
                     VGNDSYLNLENYFQTEGLAYRFVPIKAAADPSGQTGSVAAERMYDNMMNKFMWGNMKD
                     ERVYLDQNNLNMTMNFRNNFARLSETLLQENEPDSAVKVLDKCLVEMPDKTVPYNIMM
                     LRIAELYYRSTMSNALPDSLQMLSNDVELKKTDSKESIEKGNSIVKRLADIYQNDLQY
                     YLSLKGTPYAKGVERETNQALAVFQELIRLSKLKDQKAITDDLQKRFDQLEAQYSR"
     gene            complement(5053..>5812)
                     /locus_tag="PK134_04755"
     CDS             complement(5053..>5812)
                     /locus_tag="PK134_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB38191.1"
                     /translation="VVTVSSNLLTDYQSREAEVVSNVMAAVKQFPKTEGVEIKFAGSQ
                     EEQAETMSFLGTALMISLGLIFMILVIQFNSIGKPVIILSEIVFSIIGVLLGFAIFKM
                     GFSIVMTGIGVVALAGIVVRNGILLVEFTELLREQGVPTFEAIVEAGRTRMTPVILTA
                     TATSLGLIPLAVGLNIDFVKLFTEFNPHIFFGGDSTAFWGPLSWTMIFGLMFATFLTL
                     FLVPVMYLMSENLKAKIYKRPKPLMKKPDDELIA"
BASE COUNT         1816 a         1069 c         1148 g         1679 t
ORIGIN      
        1 gtatattagc ctttgtattt aatatataga aacacctatt gacacaggca acggccgcta
       61 cggttacggt gtctatgaaa cagatttggg tacagaaaca attaacttta ctttggcacc
      121 taaccctgca aaggataacg taacagcaac ctttgatttg ataaatgaaa ctgatgaagc
      181 aaccataacc ataaccgata tgcaaggcag aaaagtgttt gataaaaaag caacagcagg
      241 gcaatatgaa atgcaacttg atgtaagccc tttaagcaga gcaagctatg tagcagagtt
      301 atggataaac ggcacaaggg ttggtagaaa taaattactg ttgattaaaa attagaagag
      361 aaaaaatgcc tgcctaaaac tatgggcagg cattttaaat ttgttatgta tgaggataat
      421 aaaactcttt attttatttt gtgtagttac aacacaaacg caagcgcaaa tacaatttga
      481 caaacgctat ccgttagggt tggctagtgt aggcagaaaa gtagtagaga tttctggagg
      541 ttatatttgt gccggatttg aaatatcgga cacaaatacg tataacaata atcttatagt
      601 taagtctgat actaatggcg atactgtaaa aacactttac attccaccgc ctgattctac
      661 ttactggaac ttgtacaatg aagacccgga tgatattttt acagatttaa tagttaccaa
      721 tgacagtaat ttggtagctg tggggcgctc gcaggcatac aacccaacaa actactatga
      781 ttatgatatt ttattggtga aactaaatta taacttagac accttgtggt taaaaacata
      841 ttcacatcca acagacagtg catttcaacc acaaagcatc atacaaacca gcgatggagg
      901 ttttttaata tgcggtaagc aacgtagttt ttacaatacc aattggttcg gctttttata
      961 caaggttgat agcgcaggca attttcaatg gtataaaagt tataacaacc ccgggcctta
     1021 tcaggcaata tggggagtga tagaattgcc tgatcatagt tttatgtgta nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttgggaca taaaaaccac tgccgatggc
     1201 ggttatatta tgtgcgggca aacaggtggc tgcaacaaca atggcggcac atgtttttgg
     1261 cttgtaaaaa cagatagctt aggtttgttg gttacaggca taaaagaaaa agaaattttt
     1321 aaaacagcaa cgcttacaat ttaccctaat cctgcaacaa cctattgtac aattacagcc
     1381 atagtgcctc aaagcaacag cacgggcatt ggcaaaaaag gcgcaagcat tattttgttt
     1441 gacatgcgtg gcaaacaact tttggagcaa cccttgcaaa caggcagtaa cacagtgcag
     1501 cttaatttaa caaactattc tgcgggcaca taccttgctg tgttggtgtt agacgggtat
     1561 aacgcagcac gggttaaggt ggtgaagcaa tgacatgttt ttcttgctac cacttaaaga
     1621 ttcatagtgg tacctaaagc ttatttaaaa ttaaaagcca ttcagaagca taatttgcta
     1681 ttttcgcacg cttgtaacct tctttgtttt acatgaaaca atatacaaaa ttcaacaaca
     1741 tttccggctg ggcagttttt gccattgcag catgggtatt tctgtcaacc attgaaatga
     1801 caggtagttt ttgggactgt ggcgagttta ttgctgcggc ctacaaacta cagataggcc
     1861 atcctcccgg agcgccatta tttctgatgc tctcacgcat tatgactctt tttgcaggtg
     1921 ccgatgtaaa agatgtgccg ctaatggtta acgcattcag tgctattatg agtgcatttg
     1981 ccgtattgtt taccttctgg acaattactg ccattgcaag aaaaattgtt ttcgtaaaaa
     2041 atcaggagcc tacattagca cagattttaa ccgtagttgc cagtggcgtt gtaggtgctc
     2101 tggcatttac tttcacagat tcattctggt tttctgccgt tgaaggcgag gtttatgcaa
     2161 catcacagtt ctttacctgt attgttgttt gggctgtatt taaatgggaa aatgttgcag
     2221 acgaaaagca cagcatccgt tggataatcc tcattgccta cctcatgggc ttgtctattg
     2281 gtgttcactt gctgaactta ctttgtattc ctgctttggc attggtatat tacttccgca
     2341 aatatccgtt ttcgtggatg ggtgttgcaa aaactatggg tatagcattg gtaattttaa
     2401 tggttgtaca atatggaatt attgcagggt ttgtaaacat aggcgcaaaa attgatttat
     2461 ggttcaatca gtcaggctca tctttatgga caggatttgt tgtttactct ctcatcattt
     2521 tagctgcact gacttatggt atttactatt ctcagaaaaa acaaaaagca ctgctcaaca
     2581 ctattttgct gagctttgca tttctgttgt tgggatacac ttcctatgca cagattgtag
     2641 tgcgctcctt agccaacccg ccaatggacg aaaacaatcc ggagaatgca tttgcactat
     2701 taggctacct caaccgcgag cagtatggcg acagaccttt aggctatgga caatattacg
     2761 atgccaaagt tgtaggtcag acagaaggcg caatgagcta caccgctatt gacggaaaat
     2821 atattccgac aggagccaag gttattcccg aatacgatcc tgcacgttgt accgtttttc
     2881 caagaatgta cagccgagag cctaaccatg tttcggcata caaagaatgg tcaggaataa
     2941 aaggtgaaca aaagcctgag ttcagcaaca acctcaaata ttttttcagc tatcagatga
     3001 accacatgtt ctggcgttat tttatgtgga attttgtagg ccgtcagaac gatatacagg
     3061 gacatggcag catattaaaa ggaaactgga taagcggcat accatttatt gacagtatgt
     3121 ttttaggtcc gcaggacaaa ctacccgaca gtatgaaaaa taataaggca agaaatacat
     3181 tttacttctt gccactgttg ttaggtcttg caggattggt ttatcactat aaaaaagaca
     3241 tgaacaatgc caatgttgtg atgttgcttt tcatcatgac aggccttgca attgtaattt
     3301 acctgaatca gacaccttat cagccccgcg aacgtgatta tgcttatgca ggagcctttt
     3361 atgcattttg tatttggata ggtttgggag tggcagcaat tgccgatgct ttaaaaaagc
     3421 gaatgcctga aacaactgcg gcaatgctgg ctacgttgct ctgccttgtt gtgccaacag
     3481 ttatggctaa agaaggatgg gatgatcaca accgctctta ccgttacacc tcacgcgatt
     3541 ttgcatacaa ctatctgaac tcctgtgctc caaatgcagt aatttttacc aatggcgata
     3601 acgatacctt cccgttgtgg tacatacagg atgttgaagg tgtgcgtacc gatgtgcgtg
     3661 ttataaacct gagtctcctc aacacagact ggtatattga tcagataaaa cgtaaggctt
     3721 atgaatcaga ccctgttcct tttacattaa atacaaaaca gtatgttcag ggaacacgtg
     3781 attatattcc attctacgac agaagtgtgc agggatacag cgacctgaaa gatattattg
     3841 aatttattac cagcgataac cccgaagcaa aagtgcgcag cgaaggtggt gatgaattta
     3901 attatctgcc tacaaaaaag ttcagaataa aagttgacaa acaagctgtc attaagaaca
     3961 atgtggtgga ggcaaaagat acaagcaaga ttgttgatta tattgaattt gaaatcagca
     4021 aaaactatat catgaaagct gacctgatga tattggatct tattgcacac aatgactgga
     4081 cacgtcctat ttattttgca gttaccgttg gcaatgattc ttatcttaat cttgagaatt
     4141 attttcagac agaaggattg gcttatcgtt ttgttccgat taaagctgct gccgacccat
     4201 ccggacagac aggaagtgtt gccgcagaac gcatgtacga caatatgatg aataagttta
     4261 tgtggggtaa tatgaaagat gaaagagtat atctcgatca gaacaacctg aacatgacga
     4321 tgaacttccg taataatttt gcacgtctga gcgaaacatt acttcaagaa aacgagcctg
     4381 attctgcagt gaaagttctt gacaaatgtc tggtagaaat gcctgataaa acagttccat
     4441 ataacattat gatgttgcga attgcagaat tgtattatcg cagtacaatg agtaatgcat
     4501 tgcctgacag tttgcagatg cttagcaatg atgttgaact gaagaaaaca gattcaaaag
     4561 agagtattga aaaaggtaat tcaattgtaa aacgactggc agatatttat cagaacgatt
     4621 tgcaatatta tttatcgctt aaaggaacac cttatgccaa aggtgtagag cgcgaaacca
     4681 atcaggcgct ggcagttttt caggagttaa tcaggctgtc gaaactcaaa gaccagaaag
     4741 ctattactga cgacttgcag aaaaggtttg atcagttaga agctcagtat tcgagataaa
     4801 aaatagtgat taaaaaaagg ctgctctgat ttagagcagc cttttttatt ttggaaaaat
     4861 caactatgac gtaatgagag actattttaa aatgtgaatt aaaattcgac aacaaagtaa
     4921 tgcttatggt aatttgcaaa tagtaccttt tttttggggg gaactccaaa gtaaatatac
     4981 cataaatcaa aagaccatct caaaaattgt tctgagacgg ccttcttcaa ttataaactt
     5041 taaaaaattc tatcaggcaa tcaattcatc atccggtttt ttcattaacg gctttggtct
     5101 tttgtaaatt tttgccttca gattttctga catcagatac attaccggaa ctaaaaacag
     5161 tgtaaggaat gttgcaaaca tcaatccgaa tatcattgtc catgatagtg gcccccagaa
     5221 ggctgtgctg tcgccaccaa agaaaatgtg aggattaaac tccgtaaaca attttacgaa
     5281 gtcaatattc aaacctacag caagcggaat tagtcctaat gatgttgcag tggctgtaag
     5341 tataacagga gtcatccttg tgcgaccggc ttctacaatg gcttcaaatg ttggaacacc
     5401 ttgttcgcgc agtaactctg taaactctac caacagaatt ccatttctaa caacaatacc
     5461 tgcaagtgct acaacaccta taccagtcat cactatcgag aatcccattt taaaaattgc
     5521 aaatccaagt aatacaccta tgatactaaa tacaatttct gacaaaataa ttaccggctt
     5581 gccaatagag ttaaactgaa ttaccaatat cataaatatc aatcctaaag aaatcattaa
     5641 tgccgttccg agaaatgaca tggtttctgc ctgttcttcc tgcgagcctg caaattttat
     5701 ttctactcct tctgtttttg gaaattgttt tacggcagcc attacattac tcaccacttc
     5761 tgcttcgcga ctttgataat cggtaagcag gtttgatgaa actgttacta ca
//
LOCUS       DAORQT010000100         5804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2287_length_5804_cov_10.570360, whole genome shotgun sequence.
ACCESSION   DAORQT010000100 DAORQT010000000
VERSION     DAORQT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_5804_cov_10.570360"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PK134_04760"
     CDS             <1..336
                     /locus_tag="PK134_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38192.1"
                     /translation="IDTLLYNSQGYNASYIYIDDVSLILTPCTGIEEQNLNSNIKIYP
                     NPLSDILNIKAWGFSEIILYDVAARKLLQQKFTNSTTVNTEQLAKGLYLYEVRNKNGL
                     CKKGKVVKE"
     gene            841..2175
                     /locus_tag="PK134_04765"
     CDS             841..2175
                     /locus_tag="PK134_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HRB38193.1"
                     /translation="MTQLLFIETFVLGASAIAAIYHLVLYIQQRDKFLLFYSIHLLTL
                     SAYILFKLLSSNYNPFLPTGNLWNYITEETLQGAMVTVYVIFAAITLDVAFSRSNART
                     VMYIFFGFCILFYSYQLYNAFEYGPGVKSIQAYMVTRLSLVGIAAIALIFAWTIRTSI
                     FHRTIIIGSLVYDFSWLVSIISLSTQTTILGLNGIEIYLLGCLLDIIIFSSALGYRVK
                     TMAEEKNELVRKEAEARLAIEKTRTGIAMNLHDDIGSVLSSMSVYGEAAKRALKENNV
                     AKVDVLIDKIGVNARETMDNMSDIVWTINPVNDNGFKLFNRMESFASSVLNAKEIELK
                     FEVGKELYEKGFLMPVRQNVFFIFKEIITNCVKHSGATNVEAIFTLNGSNLLLLVSDN
                     GKGFDTKNNHLKNASGGNGLKNIKERASVLNGTIEVKSSANGTETKLEFPLT"
     gene            2266..2919
                     /locus_tag="PK134_04770"
     CDS             2266..2919
                     /locus_tag="PK134_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB38194.1"
                     /translation="MAVRVTIFDDNKRLLDSLSVLIDGSPGFQMAGIFENCNNLISDI
                     ERTQPDVILMDIEMPGINGIEAVKMVKHRFPKMNVMMQTAFENDENVFAAICAGASGY
                     ILKNTPPSKILECIIEVYQGGSPMSPSVARKVLGFLQQPKPDEKKETTVENYNLSARE
                     KEILACLVKGMSYKMIAEQCNITYETVRSHMKNIYEKLHVASMTEAVAKAINQKLVS"
     gene            3061..4755
                     /locus_tag="PK134_04775"
     CDS             3061..4755
                     /locus_tag="PK134_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38195.1"
                     /translation="MKKLLFTLMLLSSFALQAQNFTYNRFFNGGDNSAITLGELAKEV
                     IVQPDGKVISCGYSYDFACNCFHIVMFRVDVCGNIDSTFGTNGTVSYTFEDRNTGLDF
                     LMQANGKIVVVGTQAPSNASSQHNPYIARFLNNGQPDTTFGTMGTTKVIVNPACWEFT
                     HVTEVADGKYLAQNGNYIMRFDSTGAVDLSFGNSGVINYTIPSNVPLVSSWRSVQRSS
                     DKKIISVTAAWLGSGNDMRPLFMCTDTLGQLDSTFGTNGFLLDNNVILPNYQSCHIVL
                     QNDDKILSAHHNAAETEITVARYNTNGSLDTTYGTNGYVNYTAPTSPFRARDVNILSN
                     NNLLVMADAAGLDRQINTFDTNGVMTNSVTFFGNNNYNVSGSSVPLVLHVENDDEIYI
                     VSQTGGTSGMWYISSLTTTPAPTISQNFTSLSANYNLTGTTYQWYLNGNIINGATDST
                     YTFTQNGVYSVTVTIASGCDYSYSYTVTNTGIANAANLNNIKVSPNPFNDYINISNTT
                     GQLTTLTLFDAAGRTVILETTHEPDYKKQLQQLPKGMYFLTIESGSQSKHIKLLKH"
     gene            4874..>5804
                     /locus_tag="PK134_04780"
     CDS             4874..>5804
                     /locus_tag="PK134_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38196.1"
                     /translation="MHVKIFSVLICIVLLNTNSAFAQGVGINNPTPDATALLDLTSTT
                     KGLLIPRMMTFQRLSIPAPANGLMVYDITLNAFYYSDNGIWKPMDNSWQLNGNTATNP
                     VTDFIGTTDFTPLVFRTNGFEAMRITQDGRIGINQPFSPAFNAILDIQSNDKGILIPR
                     MSSPLRSNISSPPDGLLVYDSGSNEFYYSISSVWMRLLSNNTGWTTRGNAFTNPATDF
                     IGTTDNTDLVFGTFGGARGCITSAGDWGIGTIFPSAKLHVQGSYRLEDGTQGLGKLLM
                     SDATGNASWKTFEDLITGKVANGLYFNTIQQRIL"
BASE COUNT         1848 a         1119 c         1136 g         1701 t
ORIGIN      
        1 attgatactt tgctttataa ttcacaaggt tataatgcat cttatatata catagatgat
       61 gtttccctca ttctcactcc ttgcacgggt attgaagaac aaaatttaaa ttcaaatata
      121 aaaatctatc caaatcctct atcagatata ttaaatatta aagcgtgggg gttttcagaa
      181 attatccttt atgatgttgc agcaagaaaa ttattgcaac aaaaatttac aaactcaaca
      241 actgtaaaca ctgaacaact tgcgaagggt ttatatcttt acgaggtgcg aaacaaaaat
      301 ggtttgtgca agaaaggaaa agttgtgaaa gaatgaagtg ggacaacttc tcgcgagaga
      361 aaaacggctg ataacatcta agactcttgt aggcgcgatc gtcagttccg ctcgtgccat
      421 ttagctctta cgacttccag tcgcattcaa aatttgctat gtatttatgt ggtttcatag
      481 tctcaccgat ttttcatcgg gggactatga aacggggatt gtaatttcac ctttcaggtt
      541 gtgcagcccc tgacagtgtg atttaaattt caaccttcaa aactttcaaa ctttccaaac
      601 cttcgaaaca tactttgaaa taataaaaaa acattgaaga gttaatacgg tttgaatccg
      661 tttcatagtc acagcgaagg ggcgggagac tatgaaactg caaaagtgtt ttgtttgtta
      721 ccgttgccac tgcaaatatt gctgtgcgaa ttagccttga tttgttgact ataaaagtta
      781 atcgttagtt ttgagcagtt gtgcaacagg cgcagaagtt tgttgtcaat aattatttgc
      841 atgacacaac ttctttttat tgaaactttt gtattgggtg cttctgccat tgcagccatt
      901 tatcatttag tgttgtatat acaacagcgc gataagtttt tgctttttta cagcatacat
      961 ttacttaccc tctctgccta cattttattt aaactgttaa gcagtaacta caatccgttt
     1021 ttaccaaccg gcaatttgtg gaattatata acagaagaaa cgttgcaagg tgcaatggtt
     1081 actgtgtatg taatatttgc tgctattaca cttgatgttg ctttcagcag aagcaatgca
     1141 cgtacggtta tgtacatctt ttttggtttc tgtattctat tttacagtta tcaattatac
     1201 aatgcttttg aatatggccc gggagtaaaa tcaattcaag cctatatggt aacaaggtta
     1261 tcgcttgtag gcatagctgc catagcctta atttttgcat ggaccatacg tacttccatt
     1321 ttccatcgca ctatcattat cgggtctctt gtttatgatt tctcatggtt ggttagcatt
     1381 atttctttgt caacacaaac tactattttg ggtttaaatg gaatagagat ttacctgtta
     1441 ggatgcctgt tagacattat tattttttcg agtgctttag gatatagggt aaaaacaatg
     1501 gcagaagaaa aaaatgaatt ggtaagaaag gaagcagaag cccgtttggc catagaaaaa
     1561 accagaacag gaattgccat gaacctacat gacgacattg gttccgtact gagcagcatg
     1621 agtgtgtatg gcgaagcagc caaacgtgcg ctcaaagaaa ataatgtggc taaggttgat
     1681 gttttgatag acaagattgg agttaatgcc cgcgaaacca tggacaacat gagtgatatt
     1741 gtatggacaa taaaccctgt caacgataat ggatttaaac tttttaatag aatggagtca
     1801 tttgcctcat ccgttttaaa tgcaaaagaa attgaactga agtttgaagt gggtaaagaa
     1861 ttatatgaaa aaggtttttt aatgccggta agacaaaatg tattttttat ttttaaggaa
     1921 attattacaa actgtgtaaa acattcgggt gcaacaaatg ttgaagctat atttacttta
     1981 aatggcagta acctgttgct tttagttagc gataatggaa aaggtttcga cacaaaaaac
     2041 aatcatctta aaaacgcatc aggcggcaat ggtttaaaaa acataaaaga gcgtgcatca
     2101 gttttaaatg gaactataga agtaaaaagc tcggcaaacg gaacagaaac caaactggag
     2161 ttcccgctaa cataaaacag cctatgcccg aatttgggga tgacaccata tattaattga
     2221 acctattttt gtttcaataa aatgaaccat taaataaaac acaacatggc agtacgtgta
     2281 accatatttg acgataataa aagattgctt gattcgctat cggtgctaat tgatggcagc
     2341 ccgggttttc agatggcagg catatttgaa aactgcaata accttattag cgatatagaa
     2401 agaacacaac ctgatgttat tttaatggat attgaaatgc cgggcattaa cggaattgaa
     2461 gcggtaaaaa tggtgaagca ccgtttcccg aaaatgaatg tgatgatgca aacagctttt
     2521 gaaaacgatg aaaatgtttt tgcagctata tgtgcaggtg catcgggtta tattttaaaa
     2581 aacacaccgc cctcaaaaat tttagaatgc ataatagaag tttaccaggg tggcagccct
     2641 atgagcccaa gtgtggcgcg taaggtgctg ggctttttgc aacaacccaa gcccgatgag
     2701 aaaaaagaaa ctactgtaga aaactacaac ctttctgcac gcgaaaaaga aatattggcg
     2761 tgcttagtaa aaggcatgag ttataaaatg attgccgagc aatgcaacat tacctacgaa
     2821 accgtgcgct cgcacatgaa aaacatttac gaaaaactgc acgtggccag catgacagaa
     2881 gctgtagcca aagccattaa ccagaaatta gtttcgtagt aaactaactc aactctttgt
     2941 aaaagcgcac tccaaaacag tgtgcttttt tattatcccc gaattagggg attgatttcg
     3001 gccttgccac aacccatttt tgctgcatca aacaaggtca aactatataa ataaaatcaa
     3061 atgaaaaaat tgctattcac cttaatgctg ctaagcagct ttgcattaca agcacaaaat
     3121 tttacttaca accgattttt taacggaggc gacaattcag caataaccct gggcgaatta
     3181 gccaaagaag taattgtaca gcctgatgga aaagttataa gttgcggtta ctcttatgat
     3241 tttgcatgca actgctttca cattgttatg ttcagagtag atgtttgcgg taacattgat
     3301 tctacctttg gcacaaacgg cacagtatct tacacttttg aggaccgcaa cacaggttta
     3361 gattttctaa tgcaggcaaa tggaaaaata gtagtagtgg gcacgcaggc accaagtaat
     3421 gccagcagtc agcataaccc ttatatagca cgctttttaa acaacggcca gcccgatacc
     3481 acctttggaa ccatgggtac aactaaagta attgttaacc ctgcttgttg ggaatttacc
     3541 cacgttaccg aagtggcaga tgggaagtat ctcgctcaaa acggtaatta catcatgcgc
     3601 tttgacagca ctggtgctgt tgatttatca tttggaaata gcggtgttat aaattatacc
     3661 attccatcaa atgttccgct tgtgagctcc tggaggagtg tgcaacgcag cagcgataaa
     3721 aaaataattt cggtaactgc tgcatggtta ggtagcggca atgatatgcg cccattgttt
     3781 atgtgtaccg atacccttgg gcagttagat tccacttttg gtaccaatgg ctttttactt
     3841 gataataacg ttatcttacc taactatcag tcttgccata ttgtgttaca aaacgatgat
     3901 aaaattctgt ctgcacacca caacgcggca gaaactgaaa ttacggttgc gcggtacaac
     3961 accaatggtt ctttagacac aacttatggt accaacggat atgtaaacta tacagcgccc
     4021 acctcaccat tcagagcaag agatgtaaat atacttagca ataacaattt gttggttatg
     4081 gccgatgccg cagggcttga ccgtcaaata aacacttttg atacaaacgg ggtaatgacc
     4141 aacagcgtta ctttttttgg aaacaataat tataatgtat caggcagcag tgtgccattg
     4201 gtattgcatg ttgaaaacga tgatgaaata tacatagtaa gccaaaccgg tggtactagt
     4261 ggcatgtggt atatttcaag cctaaccaca acccctgctc caactatttc acaaaacttt
     4321 acatcgctaa gtgcaaatta caatttaaca ggcactactt accaatggta tttgaatggc
     4381 aatattatta atggcgcaac ggatagcact tatacattta cacaaaacgg ggtttatagt
     4441 gttacagtaa ccattgcctc aggctgcgat tattcgtatt catacacggt aaccaacacc
     4501 ggaattgcta atgctgctaa ccttaacaac attaaggttt cgcccaatcc gtttaacgac
     4561 tatataaaca ttagcaatac aaccggccag cttacaaccc ttactttatt tgatgccgca
     4621 ggccgtacag taattttaga aaccacccat gagcctgatt ataaaaagca actacaacaa
     4681 ctgccaaaag gcatgtattt tctaacgatt gaatccggca gccaatccaa gcatatcaag
     4741 cttctgaaac actaaataca accctgcaac agcgcacggc cgcaagtccg tgcgctgttt
     4801 taatatcccc gaattatggg attgaccaag cctacctccc aaaatatttt tgttacctca
     4861 actgccggca attatgcatg taaaaatatt tagtgtactt atatgtattg tactcttaaa
     4921 cacaaacagt gcctttgcac aaggcgtggg aattaacaat ccaacgcccg atgccacagc
     4981 cctgcttgat ttaaccagca ctaccaaagg tttgctcata ccacggatga tgacttttca
     5041 gcggctatcc atacccgcac ctgccaatgg cttaatggtt tacgacatta cattgaatgc
     5101 tttctattat tcggacaatg gcatttggaa acccatggat aatagctggc agctaaatgg
     5161 gaatacagct accaatcctg ttactgactt tattggaaca acggatttta cgcctttggt
     5221 gtttcgtact aacggttttg aggcgatgcg gattacacaa gatggcagaa ttggcatcaa
     5281 ccagccattt tctccagcat ttaatgccat tcttgatata caaagtaacg ataaaggcat
     5341 acttatacct cgcatgtctt cacccctgcg cagtaatatt tcttctcctc ctgacggcct
     5401 attggtatat gacagtggtt caaatgaatt ttattattct atatcttctg tttggatgag
     5461 attgctttct aacaatacgg ggtggaccac tagaggaaat gctttcacca atccagccac
     5521 tgattttata ggtacaacag ataataccga tttagtgttt ggtacatttg gtggagcacg
     5581 agggtgtata acttcagccg gtgattgggg cataggtacc atttttcctt ctgcaaagct
     5641 gcatgtgcag ggcagttacc ggttagaaga tgggacacaa ggcctgggaa aattattaat
     5701 gagtgatgct acaggcaatg caagctggaa aacatttgaa gaccttatta ccggcaaggt
     5761 agctaatggg ctttacttta atactataca acaaaggata ctta
//
LOCUS       DAORQT010000101         5774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2306_length_5774_cov_9.661654, whole genome shotgun sequence.
ACCESSION   DAORQT010000101 DAORQT010000000
VERSION     DAORQT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2306_length_5774_cov_9.661654"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK134_04785"
     CDS             <1..248
                     /locus_tag="PK134_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRB38197.1"
                     /translation="MMETTDGHTIRVRFKDYEFFVPKDCAGKTAVIEGIAFNDVTSVA
                     ELRHYAEDANKSKEEIEKITQPEKKPAFEAEGVIIYE"
     gene            241..687
                     /locus_tag="PK134_04790"
     CDS             241..687
                     /locus_tag="PK134_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38198.1"
                     /translation="MNKIFVIAGIMLLLSVSSYQYKTGWTNEAFADTTDINPNKSSEL
                     SLLMRQMYDHAAEARKAVSEQRTVSYPKTFLNINTAKPTDDKTKNEYYTTFADLYLQT
                     LDSYENSTNTNRVKSFNNVVNACLACHSSHCPGPVPKIKRLLIPLD"
     gene            689..1444
                     /locus_tag="PK134_04795"
     CDS             689..1444
                     /locus_tag="PK134_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38199.1"
                     /translation="MQVRVFLFAILISFSSLQSAAQALVRDSAIIIPFFGIGGEFHLP
                     GGDMKERFGADYGVQGVFGVKTPKNFLYSIQGGFFFGNKINQPGLLAGIASSYGTIIG
                     SDGRTADVRLFERGYHISAQAGKVFAFKKPNPNSGIWISAGAGFMQHKIRIETIGNTV
                     KGLSKKYKKGYDRLTNGIIVEEFVGYVYYGNKKLINFYAGFNFIQGFTQGRRDYQFDT
                     MKADDQSRVDLLYGFKAGWILPFYTNPDKFYYY"
     gene            1447..2682
                     /locus_tag="PK134_04800"
     CDS             1447..2682
                     /locus_tag="PK134_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRB38200.1"
                     /translation="MLLLYSIGIRLYHLAAALASPFNDKAKAFTEGRKGLFQRVQQEL
                     KNETAKHIWFHCSSYGEYEQGKPVLQQIKKQFPEYKIALTFFSPSGYKQGIKENLADY
                     IFYLPCDSSNNARQWLDYINPHMAIFVKYDFWFHYMKELNKRSIPFIYISAIFRIDQF
                     FFKWYGGAFLKELKKATRFFVQNKSSLNILKQEGITQVEVSGDTRFDRVIQLPELAFT
                     DEKIESFIAEKRVIVAGSTWPEDHKLLAAYCNSEEIKLILVPHEIHESSFKDFVSACK
                     KNVMCYSKASQEELIKADVLIIDKIGMLSKIYRFATITYIGGGFGKGIHNTLEAAVYG
                     KPVIFGPNYQKFEEAKQLIESGGAFTINNFIEMKTVFDDLLNNNRKLAQAGEISQNVV
                     KSNAGATSIIANWLQNQLQ"
     gene            2747..2820
                     /locus_tag="PK134_04805"
     tRNA            2747..2820
                     /locus_tag="PK134_04805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2781..2783,aa:Met,seq:cat)
     gene            complement(2876..3316)
                     /locus_tag="PK134_04810"
     CDS             complement(2876..3316)
                     /locus_tag="PK134_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB38201.1"
                     /translation="MASINPHINFNGNAEEAFNFYKSVFGGEFAKIVRFKDLASPEMP
                     IDKKEENKIMHIALPIGKHNVLMANDVPEFMGRTNENENRSKIVISAETKEEADKLFN
                     GLSAGGTVEMPIADSPWGSYFGMFRDKYGIEWMVDFDSRYKGQI"
     gene            complement(3482..4285)
                     /locus_tag="PK134_04815"
     CDS             complement(3482..4285)
                     /locus_tag="PK134_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HRB38202.1"
                     /translation="MNRKYLLLTIFLFSCASQKNLNNSTVQHNSLTADGRLFTSVFQQ
                     QSAEYKALCFQAYNIAELRLDQAIGSTTSVKPKAIITDIDETVLDNSPYSVHRALEGK
                     DYESVSWVDWTARAKADTLAGALTFFKYAASKNVEVFYLSNRAEKERVGTLENLKKFG
                     FPNADNAHLILKTDISSKEGRRQKIAETYDVLLLIGDNLADFSSLFDKKTTTERTQNV
                     QSIADEFGKKFIVLPNANYGDWEGALYHYDYKRTPAQKDSIMKSVLKNY"
     gene            complement(4335..>5208)
                     /locus_tag="PK134_04820"
     CDS             complement(4335..>5208)
                     /locus_tag="PK134_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRB38203.1"
                     /translation="RHLCKKLIGKGYSVSILSRTGFTEGEIPVFHFDTDKNEIDERAI
                     READYIVHLAGANIGEKRWTKNRKQLIVDSRVKTAQLIFEKVKEYNVKLKAFISASAI
                     GYYGAITTNKKFSETDSPYTDFLGETCRLWEKSADSFEEIGIRTVKVRTGVVLTSQGG
                     ALAKMITPVKLGIGSAIGSGKQFIPWIHIDDLCNIYIKAIEDTQMHGAYNAVAPDFKT
                     NREFTKEIAKVLKKPFWFPDVPDFIIKLLFGEMSEMILKGSSVSPDKIINAGYEFIFP
                     GLEGALKNVLKIEE"
     assembly_gap    5209..5264
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5368..>5774)
                     /locus_tag="PK134_04825"
     CDS             complement(5368..>5774)
                     /locus_tag="PK134_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38204.1"
                     /translation="VFNKVTDLQKNVESTMIFSEKYGFDKRNWIIMSTEIDNKAKVAR
                     EQINTYKSMLKKYEPIREKNELIGKISTNLFRMNEFVTACELWSQYINTLGAHDNGKT
                     ELDRVADSLIDLKKSDDNLRSFIQLYKKRNSL"
BASE COUNT         1858 a         1021 c          938 g         1901 t
ORIGIN      
        1 tgatgatgga aacaacagat gggcatacca ttcgtgttcg ttttaaagat tatgaatttt
       61 ttgtgccgaa agattgtgca ggaaaaactg ctgttattga aggtatagca tttaacgatg
      121 ttacttcagt agcagaactt cgccactatg cagaggatgc caacaaatca aaagaagaaa
      181 tagagaaaat tacacaacct gaaaaaaagc ctgcatttga agcagaagga gtaattattt
      241 atgaataaaa tatttgtcat agcaggtata atgctcttgc tttctgtttc atcctatcaa
      301 tacaaaacag gatggacaaa cgaagctttt gcagatacta ccgatattaa tcccaataaa
      361 agcagcgaac tttcactgct gatgcggcag atgtatgacc atgctgctga agcacgaaag
      421 gctgttagcg agcaacgtac tgtttcatat ccaaaaacat ttttaaacat caatacggca
      481 aagccaactg atgataaaac taagaacgag tattatacta cgtttgccga cttatatctt
      541 cagactttgg attcgtatga aaattcaaca aacacaaaca gagttaagag tttcaataat
      601 gttgtgaatg catgtctggc ttgtcatagc agtcactgcc caggacctgt accaaaaatt
      661 aaacgccttt taattccact tgattaaaat gcaggttaga gtttttttat ttgccatact
      721 gatttctttt tcttcattac aatctgctgc acaggctttg gtgcgtgatt cagctattat
      781 cattccattt tttggaatag gaggcgaatt tcatttaccc ggtggtgata tgaaagagcg
      841 ttttggcgca gactatggag tgcaaggtgt ttttggtgtt aagacaccaa agaattttct
      901 ttacagtatt caaggcggat tttttttcgg aaataaaatc aatcaaccag gattacttgc
      961 aggtattgca tcaagttatg gaacaataat aggcagcgat ggccgcactg cagatgtaag
     1021 actttttgaa agaggatatc acatcagtgc acaagccgga aaagtgtttg catttaaaaa
     1081 acctaaccct aatagtggta tttggattag tgcaggtgca ggatttatgc aacataaaat
     1141 cagaatagaa accataggaa acacagtgaa ggggctttct aaaaaataca aaaaaggtta
     1201 tgacagactg accaatggaa ttattgttga agaatttgtc ggttatgttt attatggtaa
     1261 taaaaagctg ataaactttt atgcaggctt taattttatt cagggattta cacaaggacg
     1321 cagagattat cagttcgata ccatgaaagc cgatgatcaa agccgtgttg atttgttata
     1381 tggttttaaa gccggttgga tacttccttt ctataccaac cccgataaat tttattacta
     1441 ctaatcatgt tgttgcttta ttccattgga ataagactat atcatttggc agcagcactt
     1501 gcttcgcctt ttaatgataa agcaaaggct tttactgaag gacgaaaagg tttatttcaa
     1561 agagtacaac aagaactaaa aaatgaaact gcaaaacaca tttggtttca ttgttcttcg
     1621 tatggcgaat atgagcaagg caaacctgtt ttgcaacaaa taaaaaaaca gtttccagaa
     1681 tataaaatag cacttacttt tttttctcca tcaggctaca agcaaggcat caaagaaaat
     1741 ctggctgact atatttttta tttaccttgt gatagtagca ataatgcacg tcaatggttg
     1801 gattacataa atccacacat ggcaatattt gtaaaatatg atttttggtt tcattacatg
     1861 aaagagttga ataagagaag tattccattt atttatatct ctgctatttt tagaattgat
     1921 cagttttttt tcaaatggta tggaggtgct tttcttaaag agcttaaaaa agcaacaagg
     1981 ttttttgttc agaataaaag ctctctcaat attttaaaac aagagggaat aactcaggtt
     2041 gaagtgagtg gcgacacacg ttttgacagg gtaattcaac tacctgagtt ggcatttacg
     2101 gatgaaaaaa tcgaaagttt tatagcagaa aaaagggtta ttgttgccgg cagcacctgg
     2161 cctgaagatc ataaattgct ggccgcttat tgtaattccg aagagataaa attaatattg
     2221 gtgccacatg aaatccatga aagttcattc aaagattttg tttctgcatg taaaaaaaat
     2281 gtaatgtgtt atagtaaggc ttcacaagaa gaattaataa aagccgatgt gttgataata
     2341 gataaaattg gaatgctttc taaaatttat cgttttgcaa caataaccta cattggtggt
     2401 ggctttggaa aaggaataca caacaccttg gaagcggctg tgtatggcaa acctgtaatt
     2461 tttggcccca attatcaaaa gtttgaagaa gctaaacagt tgattgaatc cggaggtgca
     2521 tttacaatca ataactttat tgaaatgaaa actgtttttg atgatttgtt aaacaacaat
     2581 agaaagttgg ctcaagcagg tgaaatcagc caaaatgtag tgaaatctaa tgcaggcgca
     2641 acatctatta ttgccaattg gcttcaaaac caattgcaat gaaggaaatt tctataattt
     2701 gtttttacaa ttgaaaggga atactacttt tgcagcctca aaatcaggcg aggtagctca
     2761 gatggttaga gcgcaggatt cataaccctg aggtcggggg ttcgactccc cccttcgcta
     2821 caagtagagg ctgttgcaat aaagtgtgac agccttattt ttttaataag caatatcaaa
     2881 tctgcccttt ataccttgaa tcaaaatcta ccatccattc aataccgtat ttatctctga
     2941 acataccaaa gtaagaaccc catggactat ctgcaatagg catctctaca gtgccaccgg
     3001 cagaaagccc gttaaataat ttatctgctt cttctttagt ttctgcactg ataacaattt
     3061 tactcctgtt ttcattttca ttggttcgcc ccataaattc agggacatca tttgccatta
     3121 atacattgtg tttgccaatg ggcaatgcaa tatgcataat tttattttct tcttttttgt
     3181 caatcggcat ctccggacta gcaagatctt tgaaacgaac aatttttgca aattctccgc
     3241 caaaaactga tttgtaaaag ttaaatgctt cttcggcatt gccattaaaa ttgatgtgtg
     3301 gattaatact tgccattttt tttgattttt taagtgttta tattatcagt aattattcta
     3361 taaaattact cttcaggtct ttgattacac tccaaaattt atcaatcaaa aaactatgga
     3421 tatatctact acaaaaaaca attattcaat atcctagtta gcttaacgag aaatgtttct
     3481 tctaataatt ctttaaaaca ctcttcataa tagaatcttt ctgtgcaggt gttctcttgt
     3541 aatcataatg atataatgcg ccctcccaat caccataatt ggcattgggc aggacaataa
     3601 attttttccc aaactcatcc gctatacttt gaacgttttg tgtgcgttct gttgttgttt
     3661 tcttatcaaa caaagaactg aaatccgcaa gattgtcacc gattaataaa agtacatcat
     3721 aagtttcagc gattttttgt cttcttccct ctttacttga aatatctgtt ttcaaaatca
     3781 aatgtgcatt atcagcatta gggaaaccga attttttcag attctccaac gttcctacac
     3841 gctctttctc tgcacgatta gaaagataaa atacttcaac attttttgat gcagcatact
     3901 taaaaaatgt cagtgcacct gcaagtgtat ctgctttggc acgtgctgtc cagtcaaccc
     3961 acgaaaccga ttcataatcc tttccttcta atgcacggtg aactgaataa ggtgaattat
     4021 ctaaaaccgt ttcgtcaata tctgtaatta ttgccttagg ttttacagaa gtagtgctgc
     4081 cgatagcctg atccaacctt aactcagcaa tgttgtatgc ctgaaaacaa agtgctttat
     4141 actctgcaga ctgttgttga aatactgaag taaacaatcg tccatctgct gttaaactat
     4201 tatgttgaac tgtactattg tttaagtttt tttgtgatgc acatgaaaaa agaaaaattg
     4261 tgaggagcaa atactttcta ttcataaaaa tatgtttgcg taaagatagg tgtaatttag
     4321 attttcaaat atttttactc ttcaatcttc aacacatttt tcaaggcacc ttcaaggccg
     4381 gggaaaataa attcatagcc tgcattaata attttatccg gtgacacgct actgcctttc
     4441 aaaatcattt cagacatttc gccaaacaac aacttaatta taaaatccgg aacatcagga
     4501 aaccaaaaag gttttttaag aactttcgca atttcttttg tgaactctct gtttgtttta
     4561 aagtcaggcg caactgcatt gtaagcacca tgcatttgtg tgtcttctat tgctttaata
     4621 taaatattac ataaatcgtc aatatgtatc cacggaataa attgcttacc acttccaatt
     4681 gcagaaccaa tacccaactt aacgggagta atcatcttag ctaatgcacc accctgactt
     4741 gtaagaacaa caccggtacg aacttttact gttcttattc caatttcctc aaaactatct
     4801 gcagatttct cccaaaggcg acaggtctca ccaagaaaat cggtgtaagg agaatcagtt
     4861 tcagaaaatt ttttatttgt tgtaatagca ccataataac caattgcaga tgcagatatg
     4921 aatgctttta gttttacatt atattctttt accttttcaa aaatcaattg cgcggtttta
     4981 acacgactat caacaatcaa ttgttttctg ttttttgtcc atctcttttc gccaatattt
     5041 gctcctgcaa gatgtactat ataatctgct tcacgaattg ctcgttcatc tatttcattt
     5101 ttatcagtat caaaatgaaa aacaggaatt tcaccttctg taaaacctgt cctgctaaga
     5161 atagaaacgc tatagccttt accaattaat tttttacaaa gatgtctgnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatactg ttgacattgg
     5281 tttattctta aattacctaa ataattaaaa aatcaacaaa atataaatcg aaaggtttta
     5341 aaaaggtttc tactgaactt ttttaagcta tagactattt ctctttttat ataactgaat
     5401 aaatgacctt agattatcat cactcttttt taaatctatt aaactatctg ctactctatc
     5461 taattctgtt ttcccattat catgagcacc taaagtattt atgtattgac tccacaattc
     5521 acaagctgta acaaattcat tcattctaaa tagattagtc gaaattttac ctatcaactc
     5581 gttcttttct ctaatcggtt catacttttt caacatggat ttataagtat taatttgttc
     5641 tcttgctacc tttgccttat tgtctatttc cgttgacata ataatccaat tcctcttatc
     5701 aaaaccatac ttttcactaa aaatcattgt tgattccaca tttttctgca aatctgtgac
     5761 tttgttgaaa acct
//
LOCUS       DAORQT010000102         5745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2322_length_5745_cov_10.857821, whole genome shotgun sequence.
ACCESSION   DAORQT010000102 DAORQT010000000
VERSION     DAORQT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_5745_cov_10.857821"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PK134_04830"
     CDS             <1..1034
                     /locus_tag="PK134_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRB38205.1"
                     /translation="VIPNREEIIKFCENIGFDYMGAIIWQKVTTSNTTGGGVQMGSYP
                     YPRNGILKLDYEFILVFKKLGDSPKPTKEQKELSKMSAEEWNTFFAGHWNFAGARQNN
                     HIAMYPEELPRRLIKMFSFVGETILDPFVGSGTTALAAKNTERNSVGFEINPAFIQII
                     KEKLEVHQLDLNATTYEFVKQKKINLDFEKEIQKLPYIFKDPHILDKKIDVKKLQFGS
                     KIDKDSKTKREEMFTVKEVLSPEKIRLNNNMTVKLIGVKEDPNINGKATSFLQEKIKG
                     KRVFLKYDNVKHDSENNLLCYLYLENKTFINAHLIKSGFVKVDIGVEFKYKEKFQSLF
                     NEVHAQADK"
     gene            1015..1713
                     /locus_tag="PK134_04835"
     CDS             1015..1713
                     /locus_tag="PK134_04835"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HRB38206.1"
                     /translation="MPKPINKRFSKEFGKKEKVLNYTTQTYQLSRPNKVGAVMALIRE
                     CQPKTIKEWEKWYFENAKTNEKTPVKITKKSLRELGERLHIKIKEIVIPEWTEAFSQL
                     TLQDCIDYIYNLTINRTYDGFIREKSVIEDNLAKKFPKVKFEESDPELDHAGDIDYLG
                     WVEKRAFGIQIKPVTSKANFGNYSPSERMKASFSSFTKKYGGKVFIVYSIDDKIKNTE
                     VIKQITAEIKKLAK"
     gene            complement(1745..3016)
                     /locus_tag="PK134_04840"
     CDS             complement(1745..3016)
                     /locus_tag="PK134_04840"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB38207.1"
                     /translation="MNIPDLKIPRVVIVGCGFGGLQLAKSLRKARVQIVMFDTNNYHT
                     FQPLLYQVATSGLEPDSIAYPIRKIFKKQKNFLFRWGKVENIIPEKNIIQTTVGELAY
                     DYLVIATGTTTNYFGNADIAQHAMPMKSVIEAINLRSLILQNFEQALLTQSLKEQEAL
                     MTFTVVGGGPTGVELAGALCELKKHVLPNDYPELDFRKMRVLLIEGTNSVLQAMSKEN
                     QHRALKYLDELGCEVWLETQVSSYNGEEVVFKNSKVLPSKNLLWAAGIKGVTISGLNK
                     DAINNRGRYVVDEYCKVKGCENIYAIGDVASMSTADYVNGHPQVAPVAIQMAQLLAEN
                     IELELRQKSLKKFKYFDKGTMATVGRNRAVTESFNIRFGGFAGWLVWMALHLMLLVGF
                     RNKVVVFINWLWNYINYDRNIRLIIPPFKRK"
     gene            complement(3094..3558)
                     /locus_tag="PK134_04845"
     CDS             complement(3094..3558)
                     /locus_tag="PK134_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB38208.1"
                     /translation="MSEPIKRNEALKPISRDHHHALLLGWKIRQGIKNKIPAERIKKY
                     VDWFWSSYLENHFELEEKNLYPVLGAEHASIKKAMQHHEKLRNLFQSKEQTIQQLDEL
                     QNELDRHIRFEERELFNEIQNAASAKQLQDMAAVHNEAFHDNYSDEFWVIKK"
     gene            complement(3555..4280)
                     /gene="ric"
                     /locus_tag="PK134_04850"
     CDS             complement(3555..4280)
                     /gene="ric"
                     /locus_tag="PK134_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRB38209.1"
                     /translation="MQITKDTIVGDLVAKDYRTATVFKQNGIDFCCNGNRTLEEVCKQ
                     KEMSVTNIIGELTQASQQASAAQTDFNSWPLDLLADYIEKKHHRYVEEKIQEIKPYLD
                     KICKVHGDHHPELYEINQLFNDSAGELAMHMKKEELILFPAVRRMVKSQMQKTTPEKP
                     QFGPINNPIQMMMHEHNNEGERFRQIEALSNKYNPPQDACNTYRVTFAMLKEFEEDLH
                     LHIHLENNILFPKSLELEKAISS"
     gene            complement(4292..5062)
                     /locus_tag="PK134_04855"
     CDS             complement(4292..5062)
                     /locus_tag="PK134_04855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB38210.1"
                     /translation="MDTKDIQKAHGHWILAKMGKKVLRPGGKELTKKLIDALHFNDSD
                     NVVEFAPGMGFTASLTLAHKLHSYVGVDADEDIVRMLSKRLQNSQASFILGNASQTNL
                     ESDSKDKVYGEAMLTMHADHRKSEIIREAHRILKKGGWYAIHELGLTDVDDTLKSKIQ
                     RDLALSIKVNARPLTENEWKHLLEQEGFAVKKVMTNGMHLLEMNRIIDDEGLMGATKI
                     GWNILTHPKARKRILEMRKVFKQYQTHMNAIAIVAEKL"
     gene            complement(5232..>5745)
                     /locus_tag="PK134_04860"
     CDS             complement(5232..>5745)
                     /locus_tag="PK134_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HRB38211.1"
                     /translation="RFIEIYLTRLENLMNEEGRKRLGNNPSIEDYFEHIYWKDAGMDR
                     KTGKKSMTLKLFEEKYLESFIALAKKVKGNTIEDKVMQMGNCSDALKTKLKEFDHMYN
                     VAWPIVHLETAQHYLDLKGENKAATGGSEWKKYLHPKFQQRKFFPSLWTKEEIANWGS
                     NEFQKTTNNK"
BASE COUNT         1782 a         1012 c          965 g         1986 t
ORIGIN      
        1 aagtcatacc aaatcgggag gagattatta agttctgtga aaatatagga tttgattaca
       61 tgggtgcaat tatctggcag aaagtcacta cttcgaacac aacaggtggt ggtgttcaaa
      121 tgggttctta tccttatccg agaaatggaa ttttaaaact agattacgag tttatacttg
      181 tttttaaaaa attgggcgat agcccaaaac ctactaagga gcaaaaagaa ctttctaaaa
      241 tgtcagctga agaatggaat accttttttg ccgggcattg gaattttgca ggagcgcgac
      301 aaaacaatca tattgcaatg taccccgaag aattaccaag aagattaatt aaaatgtttt
      361 cgtttgttgg cgaaaccatt cttgaccctt ttgtaggaag tggaacaact gctttggcag
      421 caaagaatac tgaaagaaat tctgttggtt ttgaaattaa tcctgcattt attcaaatca
      481 taaaagaaaa attggaagta catcagttag atttaaatgc tactacttat gagtttgtta
      541 agcaaaaaaa aatcaactta gattttgaaa aagaaattca aaaactccca tatattttta
      601 aagatcctca tattcttgac aaaaagatag atgtaaaaaa acttcaattt ggttctaaga
      661 ttgataagga cagtaaaact aaacgagaag aaatgtttac agttaaagaa gttcttagtc
      721 ctgaaaaaat tcgattaaac aataacatga ctgtaaagct tattggagta aaggaagacc
      781 caaacattaa tggtaaagca acttctttct tacaagagaa aataaaaggg aaacgtgtgt
      841 ttttgaaata tgataatgta aagcacgata gtgaaaataa cttactgtgt tatctatatc
      901 ttgaaaacaa aacttttatc aatgcgcatt taattaaaag tggttttgtg aaggtggata
      961 tcggggtaga atttaaatac aaagagaaat ttcaaagctt atttaacgaa gttcatgccc
     1021 aagccgataa ataaacgttt ctcgaaggaa ttcggtaaaa aggaaaaggt tcttaattat
     1081 acaacacaga cctatcaatt atcaaggccg aataaagtcg gtgctgtaat ggcactcatt
     1141 cgtgagtgtc agcctaaaac tataaaagaa tgggagaaat ggtactttga aaatgcaaag
     1201 actaacgaga aaacaccggt aaaaattact aagaagagtt taagagaact tggtgaaaga
     1261 ctgcatataa aaatcaagga gattgttatc ccggaatgga cagaagcgtt tagtcaattg
     1321 acattacaag attgtataga ttacatttac aaccttacta taaacagaac ttatgatggt
     1381 tttattcgtg agaagtcggt gattgaagat aatcttgcaa agaaatttcc aaaagttaaa
     1441 tttgaagaaa gcgacccaga acttgatcat gccggtgata ttgactactt gggctgggtt
     1501 gaaaaaagag cttttggtat tcagataaaa cctgttactt caaaagctaa cttcggcaat
     1561 tattctccgt cagaaagaat gaaagcaagt tttagtagct ttacaaaaaa gtatggtgga
     1621 aaagttttta ttgtttatag catagatgac aagattaaaa atacagaagt gataaaacag
     1681 attacagcgg aaataaagaa attagcaaag tgagtgtttt tacttaaatg attaaagcag
     1741 taatttactt ccttttaaaa ggcggaataa tcaatctgat attcctgtcg tagttaatat
     1801 agttccaaag ccaatttata aaaacaacta ctttatttct gaacccaaca agcaacatta
     1861 agtgtaaagc catccacacc aaccatcctg caaatccacc aaatcgaata ttgaaagatt
     1921 ctgttactgc acgatttctt ccaacagttg ccatagtacc tttgtcaaaa tatttgaatt
     1981 ttttcagtga cttttgtctt agttccaact caatgttttc agccaacagt tgtgccatct
     2041 gaattgcaac aggtgctact tgcgggtgtc cgttaacata gtctgctgtg ctcatggatg
     2101 caacatcacc aattgcataa atattttcac atccttttac tttacaatat tcgtcaacaa
     2161 catatcgtcc acgattattg atggcatctt tatttaatcc cgaaatagta acacctttaa
     2221 ttcctgcagc ccaaagcaaa tttttacttg gcagcacttt actattttta aaaactactt
     2281 cttcaccatt ataagacgat acctgcgttt ctaaccaaac ttcacagcct aactcatcta
     2341 aatattttaa tgcacgatgt tggttttctt tactcattgc ctgtagcaca gagtttgtac
     2401 cttcaataag caacacacgc atttttctga aatcaagttc gggataatcg ttcggcagaa
     2461 catgtttttt cagttcacat aatgctcctg caagctcaac acctgtgggg ccaccaccaa
     2521 caacagtaaa tgtcataagc gcctcttgtt cttttaaact ttgtgtgagt aatgcctgtt
     2581 caaaattttg gagtattaaa ctgcggaggt tgatagcttc aatgacagat ttcattggca
     2641 tggcatgttg cgcaatgtca gcattaccaa aataatttgt tgtagtaccg gtagcaatta
     2701 ccagataatc ataagctaat tctccaactg tggtttgaat aatatttttt tcgggaataa
     2761 tattttcaac ttttccccag cggaaaagaa aattcttttg tttcttaaaa attttccgga
     2821 tgggataagc aatagaatca ggctccaagc ccgaagtggc aacctgataa agtaaaggtt
     2881 gaaaagtgtg atagttgttt gtatcaaaca tcactatttg cacacgggct ttgcgcaacg
     2941 attttgctaa ttgtaatccc ccaaagccac agcctacaat aaccacacga ggaattttca
     3001 gatcagggat attcatagaa aagttatttt aaaaacagat gttagttgta catcatgcta
     3061 tgatttagtg ccattggtct gaataccggg aaactatttt ttaattaccc aaaactcatc
     3121 actgtaattg tcatgaaatg cttcattgtg cactgcagcc atgtcctgca attgttttgc
     3181 tgatgctgca ttttgaattt cattaaacaa ctctctttct tcaaaacgaa tgtgcctgtc
     3241 aagttcattt tgtaattcat ccaattgttg aatagtctgc tccttacttt gaaaaagatt
     3301 cctaagcttt tcatgatgct gcatagcttt ttttatgctt gcatgttcag cccctaaaac
     3361 aggatacagg tttttttctt ctaactcaaa atgattttcg agatagcttg accaaaacca
     3421 atcaacatat tttttaattc tttcggcagg aattttgttt tttattccct gtctgatttt
     3481 ccagcccagt aagagggcat gatgatgatc gcgacttatt ggtttaagtg cttcatttct
     3541 tttaatcggc tcactcatga tgaaattgct ttttcaagtt ctaatgattt tgggaaaaga
     3601 atattgtttt ctaaatgtat atgtagatgt aaatcttctt caaattcttt gagcatggca
     3661 aacgttactc tgtaggtgtt gcatgcatct tgtggtggat tgtacttatt gcttaacgct
     3721 tcaatttgtc tgaaacgctc accttcatta ttgtgctcat gcatcatcat ttgaatcgga
     3781 ttatttattg gtccgaactg tggtttttcc ggtgttgttt tttgcatttg cgattttacc
     3841 atccttctca ctgcaggaaa taaaatcaac tcttcctttt tcatgtgcat tgctaactcg
     3901 cctgcagaat cattaaaaag ctgattgatt tcatacaact caggatgatg atcaccatga
     3961 actttacaga ttttatctaa gtaaggcttt atctcctgaa ttttctcttc aacataacgg
     4021 tgatgctttt tctcaatata gtcagcaagt aaatctaatg gccatgaatt aaaatctgtt
     4081 tgtgctgcag atgcttgttg cgaagcttga gtcaactcac caattatgtt tgtaactgac
     4141 atctcttttt gtttacacac ctcttcaagt gtacggttac cattacaaca aaaatctata
     4201 ccgttttgtt taaaaactgt tgcagttcta tagtctttag caactaagtc gccaacaatt
     4261 gtgtcttttg tgatttgcat aatttttatt tttaaagttt ttctgcaact attgcaattg
     4321 cattcatgtg tgtttgatat tgtttgaaga cttttcgcat ttcaagaatt ctttttctgg
     4381 cttttggatg ggtcaggata ttccatccga tttttgttgc tcccatcaaa ccttcatcat
     4441 ctataatccg attcatttca agaagatgca tgccgttagt cattactttc tttactgcaa
     4501 aaccttcttg ctccaaaaga tgtttccact cattttcagt aagtgggcgt gcattaacct
     4561 ttattgataa agctaaatct ctctgaattt tactttttaa ggtgtcatct acatcagtta
     4621 gtcctaactc atgaatggca taccaacctc cttttttgag aatacgatgg gcttctctga
     4681 taatttcaga ttttctatga tcggcatgca ttgttagcat ggcttcgcca tagactttat
     4741 cctttgagtc gctttcgaga tttgtttgtg atgcatttcc gagaataaaa gatgcttgag
     4801 aattttgtag tcttttactt aacattctca ctatatcttc gtcagcatca acacctacgt
     4861 atgaatgtag cttgtgcgca agtgtcagcg atgctgtaaa gcccattccg ggtgcaaact
     4921 caactacatt atccgaatca ttaaaatgta atgcatcaat taatttcttt gtaagctctt
     4981 tgcctccggg gcgtagcact tttttgccca ttttggccaa tatccaatgg ccatgtgctt
     5041 tctgtatgtc tttagtatcc atgattattt tattatgttg atatttcttt ttaatattct
     5101 ataaataata atcacaagtt ttgaatctat ttgacagtgc aggtgttcag tgagaggtta
     5161 tctgaaagaa gtaaggtttt ttcagatatt aaagcaaaca aattgctttc agaatcaaaa
     5221 cggtgatatt attatttgtt atttgttgtt ttttgaaatt cgttgcttcc ccagttggct
     5281 atttcttctt tcgtccataa tgatgggaag aattttcttt gctgaaattt tggatgtaaa
     5341 tattttttcc attcacttcc acctgttgca gctttatttt ctccttttaa atcaagataa
     5401 tgttgtgctg tttcaaggtg aactataggc catgcaacat tatacatgtg atcaaactcc
     5461 tttaacttcg tttttagtgc atcagagcaa tttcccattt gcatcacttt atcttctatt
     5521 gtattgccct ttactttttt tgcaagtgca ataaaacttt ccaaatattt ttcttcaaac
     5581 agttttaatg tcattgattt ttttcctgtt ttacgatcca tgcctgcatc tttccagtag
     5641 atatgttcaa aataatcttc tattgaagga ttgttcccta atctttttct accttcttca
     5701 ttcattaaat tttccaatcg tgtcagataa atttcaataa aacgg
//
LOCUS       DAORQT010000103         5626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2393_length_5626_cov_9.883145, whole genome shotgun sequence.
ACCESSION   DAORQT010000103 DAORQT010000000
VERSION     DAORQT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2393_length_5626_cov_9.883145"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1473
                     /locus_tag="PK134_04865"
     CDS             220..1473
                     /locus_tag="PK134_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38212.1"
                     /translation="MMNRILLLMLAVISYGQSFTQCLDPYYPRIYQTYVERDISYGSD
                     TAYNNTVANLQMNIWKPIGDNNTARPIILWIHGGGFFTGNKNDMDVIAQAYAERGYVS
                     ATMSYRLGFYGNLFFGAPFTFDSSEVIRAAYRAVQDAHGAMRFLKARAAIDSSNINLA
                     FVGGASAGSITAMHFTYVDKQSEAPINGGAIGDVTTLFGNVQRNDLGSIFGRLNLNGQ
                     SSNVLAAVNLFGALLDTSFIESNTDPALFSYHQTGDGTVGCGYMPGLWNQPMNVSQNY
                     PHLFGSCVINDRMNNLGFSSQHYQALLYNGNVHGIHDDALIDTLVARFLSDILCENIL
                     STNTVDMPDIDVYPNPAKGQINISIPVKTFTCSLFDVQGRNIIPSNSLLHDYITLNTE
                     GISKGMYLLNIITDKQLISKRIIIE"
     gene            1476..1877
                     /locus_tag="PK134_04870"
     CDS             1476..1877
                     /locus_tag="PK134_04870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRB38213.1"
                     /translation="MSETITPIQIRFSDIDWMGHVNNAVYLSYIEKARIDFSERLPLN
                     INWRETGFILAQTQINYRHPALLNDNLSVRTWCTRVGTKSFDLGFEVFKTTDNNKIIL
                     ADGLTVLVCYNYRLSVSENIPEEWKKLLLKK"
     gene            1850..2650
                     /locus_tag="PK134_04875"
     CDS             1850..2650
                     /locus_tag="PK134_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38214.1"
                     /translation="MEKTTLKKIMGFRLWHCRSTGFKKLNWLLFLSQILLPIFVASFL
                     ISGCKKKSDDSPSPFLGITVYNDFKTHPDTFIFKAESTIPCNYKWNFADGSTEVSGDS
                     VMHIFDYGFYNVVLKGETAEGKTATTIKGINASPYTKANIVSLSLNSWPVHKSDGTTW
                     DSDGSAADIYCQIWNGNTYVSTTVSTNAPQGTANFNFITPFAITQFDKSIIIKVFNYN
                     FSPETDDLMETVVIEKNLTPLMTNQLPYAGQQLFNGGQTNGALNFIWQ"
     gene            2667..3956
                     /locus_tag="PK134_04880"
     CDS             2667..3956
                     /locus_tag="PK134_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB38215.1"
                     /translation="MEKNNPKVLRAWTFYDWANSVYSLSITTAIFPIYYNSITSANGS
                     DQVTFLGRTFTNTALYSYALSFSFLVIAFLSPLLSGIADYKGNKKFFMRLFCFLGSTS
                     CASLYFFKDLANLDIGIISFILASIGWAGSIVFYNAFLPEIATADMQDKVSAQGFALG
                     YIGSSLLLIFNLAMILNPSWFGITNSGMASRISFLMTGVWWFGFSLFTFKYLPEKTST
                     HNTNEQLLTKGFNELIKVLKQLQSLPMLKRFLISFFFYNMGVQTVMYVASLFGTKELH
                     MRDDELIITVLIIQFVAIAGAYLFAFLSSKAGNIKALLIAVTIWIGICWAAYFVATSV
                     QFFVLAFAVGMIMGGIQSLSRSTYSKMLPKTQDHASYFSFYDVCDKIGLVLGTVAYGY
                     IEELTGSMRNSILVLMTFFVVGLFSLLSIRNFRLQNT"
     gene            3986..4699
                     /locus_tag="PK134_04885"
     CDS             3986..4699
                     /locus_tag="PK134_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRB38216.1"
                     /translation="MIVDLLIPCYMDQVFPETANNVVKIIEKLGCGVNYNVEQTCCGR
                     TAFEEGYQKECKAVGEKLIKEFQNERYIVCPGAYCSGMIKNIYPQMFHNSALHNEYKS
                     VQKNIYEFTDFLVNILNVTDVGARFNGKVVILDSCKAINELTVKDSVRTLLSKVKGLE
                     WIESTDSCCGYSPSFTEKYEAISVDIGKRKILELTQTGANTVISTDYTCLMHLKSIAD
                     NEGSSLQFMHIADMLASGW"
     gene            complement(4779..>5626)
                     /locus_tag="PK134_04890"
     CDS             complement(4779..>5626)
                     /locus_tag="PK134_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRB38217.1"
                     /translation="MFQSNAGGAWDNAKKSFEKGVEINGQTYYKGSEPHKASVTGDTV
                     GDPFKDTSGPSMNILIKLMSIVSLIIAPHIASIGTQDSFNNKSEAKIVAPVVTSTVNS
                     TPEMDLAAMTGTISLDVSHSWMQFGISHFLTTANGTLAVDTGYINITNDLATSSIFVQ
                     LNVKSIDTKNTKRNNHLLSSDFFDAEKFPSIVFKSNKIEKAAEGSAFRYIAKGELTIK
                     NITKPVELRFNYMGALPNEYAAKDAHTFVGEADLDRIDFGVGESGPTVGNKVKATFSV
                     EGYKI"
BASE COUNT         1716 a         1054 c         1027 g         1829 t
ORIGIN      
        1 gtatgaaata atgataatga aactatgcag aacctataat tctgaattat caaacttgtc
       61 agctgtggct gattatcaaa ttatctcatt atctaattaa ctttattacc cttatcagct
      121 gtggttgatt atctcgcttt acccaatcac tcaaaaaaca ccctttgcaa atgccatcca
      181 ttaaccatac tttagtcaac agattttaat aaccttttta tgatgaatag aattttactt
      241 ttaatgcttg ccgttatttc ttacggacaa agctttacgc aatgtcttga cccttattat
      301 ccgagaatct accaaaccta tgttgaaaga gatataagtt acggaagcga cactgcctat
      361 aacaatacag tggctaatct tcaaatgaac atctggaaac ccataggcga caacaacaca
      421 gcacgcccta taattttatg gattcatggc ggaggttttt ttaccggaaa caaaaatgat
      481 atggatgtta ttgcacaggc ttatgctgaa agaggttatg tttctgcaac catgtcatat
      541 cgcttaggat tttatggaaa cctgtttttt ggtgcaccat ttacattcga ctcaagcgaa
      601 gttatcagag cagcataccg tgctgtacaa gatgcacatg gagccatgcg atttcttaaa
      661 gcacgtgcag caattgactc aagcaatatt aatcttgcat ttgtgggtgg tgccagtgca
      721 ggctccatca ccgccatgca ttttacctat gttgacaaac aaagcgaggc tccgataaat
      781 ggtggggcaa taggtgatgt aactacgctc tttggcaatg ttcaaagaaa tgatttaggt
      841 tctattttcg gaaggttgaa tcttaatgga caaagtagca atgtgcttgc agcagtaaat
      901 ctctttggcg ctttgttaga tacatcattt attgaatcca atacagaccc ggcacttttc
      961 tcataccatc agacaggcga tggcactgtt ggctgcggct atatgccggg gctttggaat
     1021 caacctatga atgtgtctca aaactatccg catctttttg gctcttgtgt gataaatgac
     1081 agaatgaata accttggttt ttcaagtcag cactatcagg cattactcta caacggcaat
     1141 gtacacggca ttcatgatga tgcgctgatt gatactcttg ttgcacgttt tttaagtgat
     1201 attctttgcg aaaatatttt aagcaccaac actgttgata tgcctgatat tgacgtttat
     1261 cccaatcctg caaaaggaca aatcaatatc agcatacccg taaaaacttt tacttgcagt
     1321 ttgtttgatg ttcagggaag aaatatcatt ccttcaaatt ctctgttgca tgattacata
     1381 actctaaata ccgaaggtat ttcaaaagga atgtatctgc tcaatattat taccgacaag
     1441 caattgattt ctaaacgcat catcatagaa taaatatgtc ggaaacaatt acccccattc
     1501 aaatccgctt ttcagacata gactggatgg gtcatgtaaa caatgccgtt tatctaagct
     1561 atattgaaaa agcaagaatt gattttagtg aacgtctgcc attaaacatc aactggcgcg
     1621 aaacaggatt tattcttgca cagacacaaa tcaactaccg gcaccctgca ttgctgaatg
     1681 ataatttatc tgtcagaaca tggtgcactc gtgtaggaac aaagagtttt gaccttggct
     1741 ttgaagtttt taaaactact gataacaata aaattattct ggcagatggg cttactgttt
     1801 tagtttgcta caattaccgt ctttctgtca gcgaaaacat tcctgaagaa tggaaaaaac
     1861 tactcttaaa aaaataatgg ggtttcggct gtggcattgc cgcagtacag gatttaaaaa
     1921 actaaattgg ctattatttc tttcacaaat attgttacca atctttgtag ccagtttttt
     1981 aatctcaggt tgcaaaaaaa agtccgatga tagtccgtca ccttttttag gtatcactgt
     2041 ttataatgac ttcaaaacac atccggacac ttttattttt aaagctgaat caacaattcc
     2101 ttgtaactat aaatggaatt ttgccgatgg cagcacagaa gtttctggcg atagcgtaat
     2161 gcacatattt gattatggat tttataatgt tgtcttaaaa ggcgaaactg cagagggtaa
     2221 aacagctaca acaataaaag gcatcaacgc atcaccttac acaaaagcta acattgtcag
     2281 tctaagtcta aatagctggc ctgttcataa atcagatgga acgacatggg atagtgatgg
     2341 ttctgcagcc gatatttact gtcagatttg gaatggaaat acttatgtca gtacaacagt
     2401 gagcaccaat gcaccacaag gcacagcaaa ttttaatttt attacaccct ttgccattac
     2461 gcagttcgac aagtcaatta ttattaaagt attcaactac aacttcagtc cggaaactga
     2521 tgatttaatg gaaacagtag tgatagaaaa aaatcttact ccgcttatga ctaatcaact
     2581 tccttatgct ggccagcaat tgtttaacgg aggtcaaacc aatggtgcat taaattttat
     2641 atggcaataa atcaacattg attttaatgg aaaaaaacaa ccctaaagtt ttacgcgcct
     2701 ggacatttta tgactgggca aactcagttt attcactaag tattaccact gctatttttc
     2761 ctatttacta caacagcatt accagtgcca acggtagcga tcaggtaaca tttctcggtc
     2821 gcactttcac aaacacagca ctttactctt atgccctatc tttttctttt ttagtgatag
     2881 catttctttc accgctgctt agtggcattg cagattataa aggaaataaa aagtttttta
     2941 tgaggctgtt ttgttttctt ggctctacat cttgtgcatc actttatttt tttaaagacc
     3001 ttgcaaatct cgatatagga atcatttctt ttattcttgc cagcattggc tgggccggaa
     3061 gtattgtctt ctacaatgct tttttaccag aaattgcaac tgccgacatg caggataaag
     3121 tgagtgcaca ggggtttgca ttaggctata ttggcagctc tcttttactg atttttaatc
     3181 tggcaatgat attaaatcca tcatggtttg gaattaccaa ctcaggcatg gcttcacgaa
     3241 tttctttttt aatgacaggc gtttggtggt ttggattttc acttttcaca tttaaatatc
     3301 ttcctgaaaa aaccagtacg cataacacaa atgaacaact actgacaaaa ggttttaatg
     3361 aattaataaa agtgcttaaa caacttcagt cgttgccaat gctaaaacga tttttgattt
     3421 cattcttctt ttacaacatg ggtgtgcaaa ccgttatgta tgttgccagt ttgtttggca
     3481 ccaaggaact gcacatgcgc gatgatgaat taatcatcac tgttctcatt attcagtttg
     3541 ttgccattgc cggtgcctat ctgtttgcat ttctttcatc aaaggcagga aacattaaag
     3601 ccttgctgat tgcagtaacc atttggattg gcatttgctg ggctgcatat tttgtggcta
     3661 catcggtgca gttttttgta ttagcatttg ctgttggtat gattatgggc ggcattcagt
     3721 cactatcgcg ttccacttat agtaaaatgc taccgaaaac acaggaccat gcctcgtatt
     3781 tcagctttta tgatgtttgt gataaaattg gtttggtatt gggaacagta gcttacggat
     3841 atattgaaga acttacaggt tccatgcgta attcaatttt agttttgatg actttttttg
     3901 tagtgggact tttttcatta cttagcatca gaaatttcag attacaaaac acctgatttt
     3961 tttatcggat atttgcaaat aaagaatgat agttgattta cttattccct gctacatgga
     4021 tcaggttttt cctgaaacag ccaataatgt tgtgaaaatt attgagaaac ttggatgtgg
     4081 cgttaactat aatgtagaac aaacatgttg cggcagaaca gcatttgaag aaggttatca
     4141 gaaagaatgt aaagccgttg gcgagaagtt gattaaagag tttcagaatg agcgatacat
     4201 tgtttgtccc ggtgcttact gttcgggaat gataaaaaat atttacccgc agatgtttca
     4261 caactcagca ttgcacaatg aatataaaag tgttcagaaa aatatttatg agtttacaga
     4321 ttttttagtc aacatactca atgtaaccga tgttggtgcg cggtttaatg gaaaggttgt
     4381 aattcttgat agctgtaaag ccattaatga actcactgta aaagattctg ttcgcaccct
     4441 gctcagcaaa gtaaaaggcc ttgaatggat tgaaagcaca gacagttgct gcggctattc
     4501 tccatcattt acagaaaagt atgaagccat ttcagtagat attggtaaga gaaaaattct
     4561 ggagttaaca caaacaggag ctaatacagt tatctctact gactatacct gtctgatgca
     4621 tttaaaaagt attgcagaca atgaaggatc atcattgcag tttatgcata ttgccgatat
     4681 gcttgcaagc ggatggtaat cgaataacga ttttttagtg ccaataaaaa aggccatccc
     4741 tttaagacag cctttctgaa ttcacactaa ctctttattt aaattttgta gccttcaaca
     4801 gagaaagtag cttttacttt attacctact gtaggccctg attcgcctac accaaagtca
     4861 attctgtcaa gatctgcctc gcccacaaaa gtatgtgcat ctttagctgc atattcattt
     4921 ggcaatgcac ccatgtagtt aaatctaagc tctacaggtt ttgtaatatt tttaatcgtt
     4981 aactcacctt tagcaatata acgaaatgct gagccctctg cggctttttc aattttgttt
     5041 gatttaaaaa ctattgaagg gaatttttca gcatcaaaaa agtcggagct taaaagatga
     5101 ttgtttcgct ttgtattttt tgtgtcaata gattttacat tcaactgaac aaaaattgaa
     5161 cttgttgcca agtcattagt gatattaata taacctgtat ctactgctaa tgttccgttt
     5221 gctgttgtca gaaagtgcga tataccaaac tgcatccagg agtggctcac gtcaaggcta
     5281 attgttcctg tcatggctgc aagatccatt tcaggtgtgc tatttactgt agaagttaca
     5341 accggagcaa caattttagc ttcgctcttg ttattaaaag aatcctgagt accaattgat
     5401 gcaatgtgtg gtgcaataat caaagaaaca atactcatca gtttaatcaa aatattcatt
     5461 gatggtcctg atgtatcttt aaatggatca ccaacagtgt caccggtaac agatgcttta
     5521 tgtggctcag agcctttgta atatgtctga ccatttattt caacaccctt ctcgaaagat
     5581 ttttttgcat tatcccatgc accaccggca ttgctctgaa acattc
//
LOCUS       DAORQT010000104         5311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2622_length_5311_cov_6.968227, whole genome shotgun sequence.
ACCESSION   DAORQT010000104 DAORQT010000000
VERSION     DAORQT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2622_length_5311_cov_6.968227"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /gene="wecB"
                     /locus_tag="PK134_04895"
     CDS             <1..988
                     /gene="wecB"
                     /locus_tag="PK134_04895"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRB38218.1"
                     /translation="ELNIPREDYNLKTGSGSHAVQTAEIMRLLESVLEKEKPNAVLVY
                     GDTNSTLAGALTAAKMHIPVVHVEAGMRSFNKSMPEEINRICCDHVSTLLFSPTKTGV
                     NNLCREGFSEHTLPPFNINNPKVYFCGDVMYDNSLYFAAQAKSQSSILSKSGIVKNDF
                     MLCTIHRDSNTDNPKNLSEIFRALIKISETQQQNIVIPLHPRTAKVLEQNLSPELFQQ
                     VNNSTLLKIIEPVSFIDMISLESECTMIITDSGGVQKESYFFQKPCIILRNETEWIEL
                     VEAGTAIIAGPDYAKIVDAYNQLASSKLSFAPLFGDGKASEFICSEMLSVLN"
     gene            1015..1719
                     /locus_tag="PK134_04900"
     CDS             1015..1719
                     /locus_tag="PK134_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38219.1"
                     /translation="MKYILSKSSYIKSLQCLKSLYFYKFNYKDRDPISAEQQAKFDRG
                     HQYGKIAHQLFPGGVDVSPPSPFEYATSAKQTHSLLLKQQPVIYEAAFIANEVIVALD
                     ILVRKGGLLHAFEVKSSTAISQTILNDAALQYYVITQCGFELEDFSIIHLKPDYKELP
                     VDDVKALFVSESVKEWCVSQQQFVEDNILAAKEIIRNKKMPNKKQGEHCNSPYPCDFK
                     NICNKSNSFDSGSLFG"
     gene            complement(1721..3298)
                     /locus_tag="PK134_04905"
     CDS             complement(1721..3298)
                     /locus_tag="PK134_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38220.1"
                     /translation="MNSPIYKWTGPMKIAANDAMVKSIDVRSAVGTGTQTPMATVLNN
                     GLTSQTFNVTMDISGGYTSTKTVSNLAFNQTQQVSFDPWTPGVTGSYNITVYSQLTTD
                     SDLSNDTLNKTTTAYNELPNYGWTSKTPATIATFGGASAFIPSVNAPLDSGYLFSDGG
                     SDLSIIQPKNEKFDEITNSWTSATDMPLGVYQFSMHGVQNMMYAIGGYSTGFVPSATN
                     YIYNSQTNSWTSGAVMPQPVGDYVSGVYNDSLIYFIGGYDGTSDLNAVQIYNPATNSW
                     SMGTPKPGTAVSGFRGGIYNNYIVVTCGYSQTLQGAIDETWIGQIDPANPTNITWQAA
                     APYPGGTMTRFAGGGTFKNSLPYVVFAAGDPTGVGSEVKDDVWAYDLNNGEWLIGPPK
                     PTACSNVSNFTGVVSNDSLYMVVVGGFDGTTTTGANEWLNLGASPFVGVKELNNQLEV
                     SVWPNPVSDILTVLMPSPLEMTKLTVFDLKGRMVKEIQANGSKIQIVVRDLDNGVYIL
                     KLSDKKNSNTARFTVMH"
     gene            complement(3295..4317)
                     /locus_tag="PK134_04910"
     CDS             complement(3295..4317)
                     /locus_tag="PK134_04910"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRB38221.1"
                     /translation="MKKSLLFFTWVSLCLTVSNQLNAQQWIAQSSNINPNNYVQFIDA
                     VDTNVVWGLSSDRNSQQNPVQEFTRTTNGGLTWTAGTITNAANHGPSCIMALNADTAW
                     VAMFNVSGGGSILRTNDGGVSWTKQTTATFTAPNGFPNVVHFFNANEGFCMGDPNGGY
                     FEIYTTTDGGNNWIRVPQANIAANLTGEFGITDVYTNYGDSTVYFGTNKGRIYNSTDR
                     GMNWTVSSTPYTGFIGAITFRDANFGIACEADNATTSTDLIVTNDGGATWSLLPTSSA
                     GFTMKQSIRYVPGTDSTFVITSPYTIYGSAFSIDNGVNWRLMDNLIHSDCDFVNATTG
                     WSGGGI"
     gene            complement(4455..>5311)
                     /locus_tag="PK134_04915"
     CDS             complement(4455..>5311)
                     /locus_tag="PK134_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRB38222.1"
                     /translation="AGSMALMDAGIKMKSTVAGIAMGLISDGKGRYAILSDILGDEDH
                     LGDMDFKVAGTRNGITATQMDLKVTGLPYEVMAQALEQARQGRLHIISEMEKTISEAR
                     PDYKPNAPRIVEFTIETDQIGAVIGPGGKIIQEIQRESGATITIEEKNNKGYVQIAAV
                     GKESMDKALERVKAIVAVPEIGTVYKGKVKNITTFGAFVEIMPGKDGLLHISEITHER
                     LATMDGVFKEGDLVEVKLIGVEEKSGKLKLSRKVLLPKPERQPGDAGKPNDGKPRERR
                     MPRENQNS"
BASE COUNT         1623 a         1123 c          918 g         1647 t
ORIGIN      
        1 tgagttgaat attccccgtg aagactacaa tttaaaaacc ggttcaggga gtcatgctgt
       61 acaaactgcg gaaatcatgc gtttgttaga atcagttctt gaaaaagaaa agcccaatgc
      121 cgttttagtt tatggcgata ccaactcaac acttgcaggt gcactgactg ctgctaaaat
      181 gcatattcct gtggttcatg tagaagcagg catgcgttca ttcaacaaat caatgcccga
      241 agagattaat agaatttgct gtgatcatgt ttcaacatta cttttttcac caacaaaaac
      301 cggtgtaaac aatctctgtc gcgaaggctt ttctgaacat acacttccac catttaacat
      361 taacaatcca aaagtatatt tctgtggtga tgtgatgtac gataactctc tgtactttgc
      421 tgcacaagct aaatcacaat catctattct atcaaaatcc gggattgtaa agaatgactt
      481 tatgctctgt accattcatc gtgacagcaa cacggataac ccaaaaaatc tttcagaaat
      541 tttcagagcg ctgatcaaaa tttctgaaac acaacaacaa aacattgtaa ttccactaca
      601 tccaagaaca gccaaggtgc tggaacaaaa cctgagcccg gaattgtttc agcaagtaaa
      661 caatagtact cttttaaaaa ttatagaacc tgtttctttt attgatatga taagtctgga
      721 atctgaatgt acaatgatta tcacagattc gggaggtgtt caaaaagaat cctatttttt
      781 ccaaaaacca tgcatcattc ttagaaatga aacagaatgg attgaattag ttgaagcagg
      841 tactgcaatc attgccggtc cggattatgc taaaattgtt gatgcttata accaactcgc
      901 ttcatcaaaa ttaagcttcg cacctttatt tggtgatggc aaggcatcag agtttatatg
      961 cagtgaaatg ctatctgtat tgaattgact acatttacat gaaataaaaa tgtcatgaag
     1021 tacattttat cgaagtcaag ttacataaaa agtttacaat gtcttaagtc gctttacttc
     1081 tataaattta actataaaga ccgtgatccg attagtgcag agcaacaagc taagtttgat
     1141 cgcggacatc aatatggaaa aattgcacat caactttttc ccggaggtgt agatgtttca
     1201 ccgccttcgc catttgagta tgcaacatca gcaaaacaaa cacattcatt attactaaaa
     1261 cagcaacctg taatttatga agctgccttt attgcaaatg aagtaattgt tgccttggat
     1321 attctggtgc gtaaaggcgg gcttctccat gcatttgagg taaaaagttc tactgctatc
     1381 agtcaaacaa ttttaaatga tgcagcttta caatattatg ttatcacaca gtgtggattt
     1441 gaactggaag attttagcat catccatctg aaacctgatt ataaagaact tcctgttgac
     1501 gatgtaaaag cactattcgt ttctgaaagt gtaaaagagt ggtgtgtgtc acaacaacaa
     1561 tttgttgaag acaacatttt agccgctaaa gaaatcatcc gtaacaaaaa aatgcccaat
     1621 aaaaaacagg gcgaacattg taattcgccc tatccttgtg attttaaaaa tatttgtaat
     1681 aagagtaact ccttcgattc aggaagttta tttggataag ttaatgcatc actgtaaatc
     1741 gtgcagtgtt tgaattcttt ttatcagaca attttaaaat gtaaacacca ttatccaaat
     1801 ctctaacaac aatttgaatc ttactaccat tagcttgaat ttctttcacc attcttcctt
     1861 ttaaatcaaa tactgttagt ttggtcattt ccaacggtga cggcattagc actgttaaaa
     1921 tatcagaaac aggatttggc caaacagaaa cttctaactg attattcaac tctttcactc
     1981 ctacaaaagg ggaagctcca agattaagcc attcatttgc acctgtagtc gtagttccat
     2041 caaaaccacc tacaacaacc atatacaaac tatcgttgct tacaactcct gtgaaattac
     2101 ttacattact gcatgcagtt ggttttggcg gacctatcag ccattcgcca ttattcaaat
     2161 cataagccca aacgtcatct tttacttctg aaccgacacc tgtagggtca cctgcagcaa
     2221 ataccacgta gggcaaactg tttttaaatg ttccaccacc tgcaaatcgt gtcattgtgc
     2281 caccgggata tggcgctgct gcctgccatg taatgtttgt tgggtttgca ggatctatct
     2341 gacctatcca tgtttcatca attgcacctt gcaaagtctg actgtatcca caagttacaa
     2401 caatataatt attataaatt cctcctctaa aaccggatac agcagtgcct ggtttaggcg
     2461 ttcccatact ccaactgttt gttgccgggt tgtatatctg aacagcattt aaatcactag
     2521 ttccatcata accgccaata aaataaatta atgaatcgtt gtaaactcca cttacatagt
     2581 cacccacagg ctgtggcatt actgcacccg atgtccaact gttagtctgt gaattataaa
     2641 tataattggt agcagaagga acaaagcccg tactataacc accaattgca tacatcatat
     2701 tttgtacacc atgcattgaa aactgataaa cgcctaaggg catatcagta gcagaagtcc
     2761 agctatttgt aatctcatca aatttttcat tcttaggctg aatgatactt aaatcacttc
     2821 cgccatcaga aaacaaatat cctgaatcaa gaggggcatt tacagaagga ataaatgccg
     2881 atgctccacc gaaggttgct attgtagcag gtgttttgct tgtccatcca tagtttggaa
     2941 gttcattata agctgttgtt gttttattta aagtgtcgtt agataaatca ctgtcagtgg
     3001 ttaattgaga ataaacggta atgttataac ttcctgttac tcccggagtc cacgggtcaa
     3061 agcttacctg ttgtgtttga ttgaatgcca gattgcttac tgttttagtt gatgtatatc
     3121 cacctgaaat atccatagta acattaaagg tttgagaagt aagtccgtta ttcagaaccg
     3181 ttgccatagg agtctgtgta cctgttccaa ctgcgctgcg aacatcaata ctcttaacca
     3241 tagcatcatt ggcagcaatt ttcatggggc ctgtccattt gtaaatgggc gaattcaaat
     3301 tccacctcca ctccaaccgg tagtggcatt tacgaagtcg caatctgaat ggatgagatt
     3361 atccatcaaa cgccagttaa caccattatc aatgctgaat gctgaaccgt aaatggtata
     3421 gggagaagtg atgacaaaag tagagtctgt tccaggtaca taacgaattg attgtttcat
     3481 agtaaaacct gcagatgaag taggcaaaag actccatgtt gcaccaccat cgttagtgac
     3541 aattaaatct gttgatgtgg ttgcattatc agcctcacat gcaattccaa agttagcatc
     3601 gcgaaaagtg atggcaccga taaaacccgt ataaggggta ctgctcacag tccagttcat
     3661 tcctctgtct gttgaattat aaatgcgtcc tttattagtt ccgaaataga cagtactgtc
     3721 gccatagttg gtatagacat cagtaatacc aaattcaccc gtcaggtttg ctgcaatatt
     3781 tgcctgaggc acacgaatcc aattgttgcc accatctgtt gttgtgtaaa tttcaaaata
     3841 tccaccattt gggtcgccca tacaaaagcc ttcgttagca ttaaaaaaat gcacaacatt
     3901 aggaaagcca ttaggcgctg taaaagttgc cgttgtttgt tttgtccagg aaacaccacc
     3961 atcattggtt cgcaaaatac ttcctcctcc actgacatta aacattgcta cccatgcagt
     4021 atcggcattg agagccataa tacatgacgg gccatgattg gctgcatttg taatagtacc
     4081 tgctgtccat gtcagtccgc cattggtcgt tcgtgtaaat tcctgaactg gattctgctg
     4141 cgaattgcgg tcagaagata atccccatac tacattggta tcaactgcat caataaattg
     4201 cacatagttg tttgggttga tgtttgagga ttgtgctatc cattgctgag catttaactg
     4261 attactaacc gtcagacaaa gagaaaccca tgtaaaaaag agtaaagact ttttcataaa
     4321 tttttgattt tagtggtttg aacagttttg atttatcaaa caaatataat aaaatcatta
     4381 aacgggctat taaaagcttt tacatcttta tatatcggtc aaaaaaaaac cggccttacg
     4441 accggtttac tgtattatga gttttgattc tctctgggca ttcttcgctc acgaggtttg
     4501 ccatcatttg gcttacctgc atcacccggt tgacgttccg gctttggcaa caacacttta
     4561 cgggataatt tcagtttacc acttttctct tctactccaa tcaatttcac ttcaactaaa
     4621 tcgccttctt taaaaacacc atccatggtt gctaatcgct catgtgtaat ttcagaaata
     4681 tgtaacagac catctttacc gggcataatt tcaacaaatg ctccgaaggt tgtaatattc
     4741 tttacttttc ctttataaac ggttccaatt tcaggaactg caacaatagc ttttacacgc
     4801 tctaatgctt tgtccatgct ctcttttcct acagcagcaa tctgcacata gcctttattg
     4861 ttcttctctt caattgtgat agtagcacca ctttcacgtt gaatctcctg aataattttt
     4921 cctccgggtc caatcactgc acctatctga tctgtttcaa ttgtaaactc aacaatacgt
     4981 ggtgcatttg gtttgtaatc aggacgcgct tctgaaatag ttttttccat ttcgctgata
     5041 atatgcaaac gtccctgacg tgcctgctct aatgcctgcg ccattacttc ataaggcaaa
     5101 cctgttacct tcaaatccat ttgtgttgca gtgataccgt tgcgtgtacc tgcaacttta
     5161 aaatccatat cacctaagtg atcttcatca ccaagtatat cactcaatat agcatagcgt
     5221 cctttgccat cgctaatcaa tcccattgca atacctgcca ctgttgattt cattttgata
     5281 cctgcatcca tcaatgccat acttcctgca c
//
LOCUS       DAORQT010000105         5309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2624_length_5309_cov_10.047202, whole genome shotgun sequence.
ACCESSION   DAORQT010000105 DAORQT010000000
VERSION     DAORQT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2624_length_5309_cov_10.047202"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK134_04920"
     CDS             <1..245
                     /locus_tag="PK134_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38223.1"
                     /translation="GPRKHGNTTLGCNSNNEAIKLKDYFIQNFPNSKITLINMTDLIN
                     KCHHLRCYCEGDNLKTVIQLMNHTNINSNGNKILNK"
     gene            251..718
                     /locus_tag="PK134_04925"
     CDS             251..718
                     /locus_tag="PK134_04925"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRB38224.1"
                     /translation="MSLNINHPAPLFESFNSEKQPFSLEQLKGKNVLLLFFPAAFTST
                     CTKELCSVRDNLSYYNNLNCTVIGISTDTIYSLAKYKEEQKLNFQLVSDFNKEIGKIY
                     HAQYELFGLGMKGITKRAAFLIDKTGILRYIEVLENASEIPDFNKIKSVLDSL"
     gene            complement(698..1714)
                     /locus_tag="PK134_04930"
     CDS             complement(698..1714)
                     /locus_tag="PK134_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB38225.1"
                     /translation="MSSIGKKLKIIGTNFAFLILFLSLAEVGLRIANLGYDNAAFEPD
                     SVLHHIHRPDYSFIHSNAAEHEYGNIPVRYDKIGCVVNPVKILNVQCNKRVWLFGDSY
                     IEALQVNYDSSVIGILEKDFPDVCFKNFAVGAYSPVLHYLKLKQLLKNDKPDVVLLFL
                     YANDAAEDKQYLNEAKLAGGEVTAVDGGMPQWKYEVIRNSNLLRLIRKFYMQMTVKLE
                     NKKYMNESALSENNAILAEPTLTYLEKFVTLCKKNNVRVIVSCIPSKTDVLRNDFNSA
                     SFSSQIKAWCTGRCDFIDLQEPLQRFYLQTNTSPYYKNDIHFNNYGHRIVAMTIAQLQ
                     TIKN"
     gene            complement(1721..3391)
                     /locus_tag="PK134_04935"
     CDS             complement(1721..3391)
                     /locus_tag="PK134_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRB38226.1"
                     /translation="MKKIYLLILIFLTSHELYSQNRYAVMLTDKNNSSFSFSNPSAFL
                     SQRAINRRLQFGIAIDSLDLPVNATYLTAIQNTGAVILNTSRWLNEVTVDVSVNPNAI
                     AAINALPFVKQTKLASRTTNRTNSKFSFEIESLMERQSQTQKVASTASFYNYGNALNQ
                     IQMLHGDNLHDLGFRGDGKIIAMLDAGFLRADSMTAFDSLRAHNRILSTYDFVDHNSN
                     VYDDHTHGSMCFSIIGANDPGNIVGTAPEASFLLYRSEDAATEKLIEEYNWATAAETA
                     DSAGTDIISSSLGYSVFEDSTMNHTYNDLDGRTAPISIAANIASRKGIVVVSAAGNEG
                     NGQWHYITAPADADSILTIGAVDAAGVYASFSGTGPSFDGRIKPSVVAQGQGTFVSDP
                     YSNTVFSGNGTSFSCPVIAGLVASLWQAIPSANNMQIINAIVQSASQYNNPDSLLGYG
                     IPNFDSARNSLLAVFNPYYGKSDFIEKIFPNPFKNQLNINFYADSNSQYTSEIFDLSG
                     KQVMMKRGKFVPYSINSINLATTAMAKGVYLLRITTEKNVFQSRILKQ"
     gene            complement(3394..4065)
                     /locus_tag="PK134_04940"
     CDS             complement(3394..4065)
                     /locus_tag="PK134_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38227.1"
                     /translation="MAKSKNIFSLIILALVLLMGCTKDKETVSAIDYRYYPIAIGDTM
                     IYQVSLFNKDLNTYDSTYQLLERVESVFNDNEGRPTYRLERYVRNTDTDAWSIYKVWT
                     TNKTTTSIEKQEDNITYIKLIFPVKLNKTWNGNAKNIFADDFDDYKIISLNEPLQSGN
                     YSFDSTLNVQQIDYDYGFEKRKHFEIYATGVGMIYKDQYDSLQGSPLGKIYHYTETLL
                     YHNKK"
     gene            complement(4052..5158)
                     /gene="mnmA"
                     /locus_tag="PK134_04945"
     CDS             complement(4052..5158)
                     /gene="mnmA"
                     /locus_tag="PK134_04945"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRB38228.1"
                     /translation="MSKKGRILVAMSGGIDSSVTAMMLHDEGYEVIGITMKTWDYATS
                     GTSGKETGCCSLDSINDARSIAVEKGFPHYILDIRGEFGDFVIDNFVDEYLAGRTPNP
                     CVLCNTHIKWEALIKRADMLDCEYIATGHYASVRNEGGRYIVSRGKDLNKDQSYVLWG
                     LSQKNLARTRFPLGNYKKSEIRNMAFDMGFKELANKSESYEICFVPDNDYRAFLKSRV
                     EGIEEKLDGGVFVNTKNEVIGKHKGYPFYTIGQRKGLEVAFGKPMYVTEIKPNTNTVV
                     LGELEDLQKTTMKVRGLNLVKYESLSNPTEVLTKIRYKDPGTMSVITQTSAVNAAVEF
                     HAPVTAVAPGQSAVFYEGNDVVGGGFIDKEYGKV"
BASE COUNT         1676 a         1034 c          833 g         1766 t
ORIGIN      
        1 cggggccaag aaagcatggt aacaccactt tgggatgtaa ttcaaacaat gaagcaatta
       61 aactaaaaga ttattttatt caaaatttcc ctaattcaaa aattactctc ataaatatga
      121 cagacttaat taataaatgt caccatttac gttgctattg cgaaggcgat aacttaaaaa
      181 cagttataca attgatgaac catacaaata ttaattcaaa cggaaataaa atattaaaca
      241 aataaatatt atgtcactaa atattaatca ccctgcacca ctttttgaaa gtttcaattc
      301 agaaaagcaa ccgttttcac ttgagcaatt aaaaggcaag aatgttttac tcctattttt
      361 tcctgcagca tttaccagta cttgtacaaa agaattgtgt agtgtcagag acaatctgtc
      421 ttattataat aacttaaact gcacagtaat tggcatctca accgatacta tttattcgtt
      481 agcaaaatat aaagaagagc agaagttgaa ttttcagtta gtctccgatt tcaacaaaga
      541 aataggtaaa atttatcatg cacaatatga gttgtttgga ttagggatga aaggtattac
      601 aaaacgtgct gcatttctca ttgataaaac cgggattcta cgttacatag aagtattaga
      661 aaatgcaagt gaaattcctg actttaataa aatcaaatca gttcttgata gtttgtaatt
      721 gcgctattgt cattgccaca atgcggtggc cgtagttatt aaaatgaata tcatttttgt
      781 aatatggtga agtgttagtt tgcaaataga atctctgtaa gggctcttgt aaatctataa
      841 aatcacatct tccggtacac catgctttta tctgacttga aaaactagca gaattaaaat
      901 catttctcag aacatctgtc tttgaaggta tgcacgacac tataactcta acattatttt
      961 tcttacaaag tgtaacaaat ttttctaaat aagttagtgt tggctctgca agaattgcat
     1021 tattctcact caaagcagac tcattcatgt actttttatt ttccagtttc acagtcatct
     1081 gcatataaaa tttacgtatc aaacgaagca ggtttgagtt cctaataact tcatatttcc
     1141 attgtggcat accaccatca acagccgtta cttctcctcc agctaacttg gcttcattaa
     1201 gatactgttt atcttctgca gcatcattgg catataaaaa caaaaggaca acatcaggtt
     1261 tatcattctt caacaattgt tttaatttta aataatgcag aactggactg tatgcaccaa
     1321 cagcaaaatt tttaaaacat acatccggaa aatctttttc taaaatacct ataacagatg
     1381 aatcataatt tacttgcaat gcttcaatat aagagtcgcc aaataaccaa actcttttat
     1441 tacactgcac attcagaatt ttaacagggt taacaacaca acctatctta tcatacctga
     1501 caggaatatt accgtactca tgctctgcag cgtttgaatg aataaacgaa taatcaggtc
     1561 tgtgaatatg atgcagaaca gaatcgggtt cgaaggctgc attgtcataa ccgagatttg
     1621 caattcgtaa tccaacctcc gcaagggata aaaacaatat taaaaaagcg aagttggttc
     1681 ctataatttt aagcttcttc cctattgatg acattgaagc ctattgtttc aaaattcgcg
     1741 actgaaaaac attcttttca gtagtgatac gcaacaaata aactcctttt gccatggctg
     1801 tggtagcaag gtttatgctg ttgatagaat atggaacaaa tttgccacgt ttcatcatta
     1861 cttgctttcc actgagatca aatatttctg aagtatattg actgttacta tctgcataaa
     1921 aatttatgtt cagttggttc ttaaatggat taggaaatat tttttcgata aaatcgcttt
     1981 taccataata tggattaaaa actgcaagta aagagttacg tgcagaatca aaattaggaa
     2041 tgccataacc cagcaatgag tcaggattgt tatactgtga tgcactctgc actatggcat
     2101 taataatttg catgttgttt gcagatggaa ttgcttgcca caatgaagcc actaatcctg
     2161 caattaccgg gcatgaaaaa gaagttccat taccggagaa aacagtattt gaataagggt
     2221 ccgaaacaaa tgtgccttgt ccttgtgcaa ctacagatgg ttttattctt ccatcaaaag
     2281 atggacctgt gccactgaat gaagcatata ctcctgctgc atcaacagcg ccaattgtta
     2341 aaatactatc ggcatctgca ggtgctgtta tgtaatgcca ttgaccatta ccttcattac
     2401 cggcagcgct aacaacaaca attccttttc gtgaagcaat gttggctgca atggatatgg
     2461 gtgctgttct gccatccaaa tcattgtaag tatgattcat tgtagaatcc tcaaacacac
     2521 tatatcctaa tgaacttgag ataatatctg ttcctgcact gtctgctgtt tctgctgctg
     2581 ttgcccagtt atattcttct atcagctttt ctgttgctgc atcctctgaa cgatacaata
     2641 gaaatgaagc ttcaggtgca gtaccaacaa tatttccggg atcattagcg ccaattattg
     2701 aaaaacacat ggaaccatgt gtgtggtcgt cataaacatt actgttatga tcaacaaaat
     2761 cataagtgga caaaattctg ttatgtgcac gcagactgtc gaatgcagtc atactgtcag
     2821 ctctaagaaa tccggcatca agcatagcaa taatttttcc atcacctctg aatcctaaat
     2881 catgtaagtt gtcgccatgc agcatttgaa tttgattgag tgcatttcca taattataaa
     2941 atgatgcagt cgaagctact ttctgtgtct gagattgacg ctccattaaa ctttctatct
     3001 caaaactgaa cttgctgttt gttctgttag ttgtgcgtga agccaatttt gtctgtttca
     3061 caaaaggcaa tgcatttatt gctgctattg catttggatt tacggataca tctactgtca
     3121 cttcatttag ccagcgtgat gtatttaaaa ttactgctcc ggtattttga attgctgtaa
     3181 gataagttgc attaaccggt aaatcgagtg aatctatagc aataccaaat tgcaatcttc
     3241 tgttaattgc tctttgagat aaaaaggcag atggattaga gaaactaaaa gaagagttat
     3301 ttttatctgt cagcattact gcataacgat tctgactata caactcatgt gatgtcagaa
     3361 aaatcagaat caacaagtaa atttttttca tgcttatttt ttattgtgat aaagtaatgt
     3421 ctctgtgtaa tgataaattt taccaagcgg acttccttgc aaagaatcat actggtcttt
     3481 gtaaatcatt cctactccgg tggcataaat ttcaaaatgc tttctttttt caaagccata
     3541 atcataatct atttgttgca catttaaagt actgtcaaat gaataatttc ctgattgcaa
     3601 tggttcattc agagaaataa ttttataatc atcaaagtca tcagcaaaaa tattttttgc
     3661 attaccattc catgttttat tgagttttac agggaaaatg agttttatat aagtaatgtt
     3721 gtcttcttgt ttctctatac tggtagtagt cttattggtt gtccacacct tataaatact
     3781 ccatgcatct gtatcggtat tacgaacata acgttctaaa cggtatgttg gccgaccttc
     3841 gttatcatta aaaacagatt ctactctttc taataactga taggtactat cataggtgtt
     3901 aaggtcttta ttaaacaaac taacctgata gatcatagta tcgccaattg ctattggata
     3961 ataacgatag tcaatggcac taactgtttc cttgtcttta gtacacccca tcaataatac
     4021 taaagccagg atgatcagtg agaaaatgtt tttagacttt gccatactct ttatcaataa
     4081 atcctcctcc tactacatcg ttgccttcgt agaaaacagc actctgtccg ggagcaacag
     4141 cagtaaccgg tgcatgaaat tcaacagcag cattgactgc agatgtttgc gttatgacac
     4201 tcatagtgcc cgggtcttta taccgtattt ttgtaagtac ttctgtggga ttactgagcg
     4261 attcatactt tacaagattt aatccacgta ccttcatagt agttttctgt aaatcttcca
     4321 gctcacccaa taccacagta tttgtgtttg gttttatttc cgtaacatac attggcttac
     4381 caaatgcaac ttcaagacct ttacgctgac caattgtata gaaaggatat cctttatgct
     4441 ttcctattac ttcatttttt gtattcacaa atacgccacc atcaagtttt tcttcaatac
     4501 cttcaactct tgattttaaa aatgctctgt agtcattatc cggaacaaaa caaatctcat
     4561 agctctcact tttattggct aattctttaa atcccatgtc gaatgccata ttgcgaattt
     4621 ctgatttttt ataattccct aatggaaatc gtgttcgcgc caaatttttc tgacttaaac
     4681 cccacaacac atagctttga tctttattta aatcttttcc tctgctgaca atgtaacgac
     4741 ctccttcatt gcgtacagat gcataatgtc cggtagcgat atactcacaa tctaacatat
     4801 cggcacgttt aattaatgct tcccatttga tatgtgtatt acaaagcaca cagggattgg
     4861 gtgttcgtcc ggcaaggtat tcatcaacaa aattatcaat cacaaaatcg ccaaactcac
     4921 ctctgatatc gagtatataa tgcggaaatc ctttttctac agcaatactt ctggcatcgt
     4981 ttattgagtc gaggctgcaa catcctgtct ctttaccgga cgttcccgat gttgcataat
     5041 cccatgtttt cattgtgatg cctataactt cataaccttc atcatgcagc atcatagctg
     5101 taacagagct atcaatacca ccactcatgg caactaaaat tctacctttc ttactcacct
     5161 tgcaaagtta ctttaattat tactgaagtg tatctgtctt aacttcaaat ccatttttat
     5221 attgactctc aatccacggc ttgccttttt cattaaaaac agtccactta cctgccctgt
     5281 ctcccgcatt atattgccct gttgtataa
//
LOCUS       DAORQT010000106         5258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2663_length_5258_cov_6.673650, whole genome shotgun sequence.
ACCESSION   DAORQT010000106 DAORQT010000000
VERSION     DAORQT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2663_length_5258_cov_6.673650"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="PK134_04950"
     CDS             <1..2278
                     /locus_tag="PK134_04950"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRB38229.1"
                     /translation="AVITVSNKETIKDVIATIKDLGYGVTTVRKKFPVLNMSCASCAV
                     SAESMIQSVNGVINASVNFATTELTVEYLPNMTNPLALQKALQEVGYDLLIEDEASQQ
                     NSFENIQQEKFKKLKQKTVLAIALSIPVVIIGMFLMDIPYANEIMWLFSTPVVLWLGK
                     DFFINAWKQAKHRSANMDTLVALSTGIAYLFSVFNMLFPQFWHQRGLHAHVYFEAAAV
                     IVAFILLGKMLEEKAKGNTSSAIKKLMGLQPKTVIVIQSDGTEKQTFVEDVIVDDIIL
                     VKPGEKIAVDGKLINGSSYVDESTLTGEPVPVLKNINDNVFAGTINQKGSFQFNALKV
                     GKETMLAQIIKTVQDAQGSKAPVQKLVDKIAGIFVPVVIGISILTFVLWQIFGGDNGL
                     VQGLLAAVTVLVIACPCALGLATPTAIMVGVGKAAENGILIKDAESLELAKKITTVVL
                     DKTGTITEGKPVVTDVYWYNDNSSTASILYSIEKKSEHPLADAVVKNFTKTEDIELTS
                     FESITGKGVRATYQKENYFVGNMTFIKEQNILIEDMKVEKATAWTKESKTVIWFADSK
                     QVLSIIAISDSIKPTSVAAIQQLHKMGIELYMLTGDNESTAKAIAEKTGIDKYQASML
                     PHQKADFIKVLQQQGKVVAMVGDGINDSTALATADVSIAMGKGSDIAMDVAKMTIVSS
                     DLNKIPMAIRLSKQTVATIRQNLFWAFIYNVIGIPIAAGILYPINGFLLNPMIAGAAM
                     AMSSVSVVSNSLRLKWKK"
     gene            2380..2604
                     /locus_tag="PK134_04955"
     CDS             2380..2604
                     /locus_tag="PK134_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRB38230.1"
                     /translation="MENKKHLQFKTNINCSGCVAAVKPHLDKLISSESWHVDTANKDK
                     ILSVSTNDISEEEIINTVQKAGYKIELLKK"
     gene            complement(2730..3251)
                     /gene="azu"
                     /locus_tag="PK134_04960"
     CDS             complement(2730..3251)
                     /gene="azu"
                     /locus_tag="PK134_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HRB38231.1"
                     /translation="MRKSLLALASAAFIFAACNQSKQPETPATETTPPAATEEAAPAV
                     APPTEPAIVEISGGDDMKFDKTEITVKEGQTVKLTLKHTGKSPKDAMGHNFVLLASGT
                     SLQDFATAAIKAKDNDYIPADMASSVIAHTKTIGGGETTEIEFTAPSKGTYDFLCSFP
                     GHYGTMKGHFIVE"
     gene            complement(3556..4737)
                     /locus_tag="PK134_04965"
     CDS             complement(3556..4737)
                     /locus_tag="PK134_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRB38232.1"
                     /translation="MNKLADIAACVIIPTYNNSKTLKKVIDGVLNFSSGNDVIVINDG
                     STDNTTEILNQYNEQIVLLNNKTNSGKGYSLRKGFKEAISRGFKNAITIDSDGQHYPE
                     DIDVMIENALKFPEALLMGSRNMNQESVPGKSSFGNKFSNFWFWVETGQKLPDTQTGF
                     RLYPLEPLKKIKLYTTKFETEIEVIVKLAWQDVKIIPVNIRVLYDMKERVSHFRPFRD
                     FTRISILNTYLVTLTLFYYLPLRLFKYIRRKGIFTIIKEEIFRRDESILKKSLSVGFG
                     VFMGIVPIWGFQLLVGIPLAVLFRLNKVLFITAANISIPPMIPLIIYFSYYLGGFFVS
                     NDMKFQSMADITLQSIHANFMQYATGACVLALISGIFFMTITYITFLALKFKDKLTEK
                     A"
     gene            complement(4734..5087)
                     /locus_tag="PK134_04970"
     CDS             complement(4734..5087)
                     /locus_tag="PK134_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38233.1"
                     /translation="MLKDFYTIINKTSGQNCVYRIKLNPAHEIFKGHFPGQPVTPGVV
                     LIQMTTELTQNAIEQNVKLKQVINSKFLNVMNPEKNPEVTFKISITKDGQLYKVATSA
                     EDEGVCFFKITAIYQ"
BASE COUNT         1757 a          815 c          969 g         1717 t
ORIGIN      
        1 tgctgtaatt actgtttcaa ataaagaaac tattaaagat gtcattgcaa caattaaaga
       61 ccttggatat ggcgtaacaa ccgtaagaaa aaagtttcca gtattaaata tgtcatgcgc
      121 ttcatgtgca gttagtgctg agagtatgat tcaatcggta aatggtgtaa ttaatgcttc
      181 tgtaaatttt gccacaacag aacttacagt tgaatatctc ccaaacatga ccaatccgct
      241 ggcattacaa aaggcattac aagaggttgg atatgatttg ctaatagaag atgaggcatc
      301 acaacagaat agttttgaaa acatacagca ggagaagttt aaaaagttaa aacagaagac
      361 tgttttagct attgcactat caattcctgt tgtaataatt ggtatgtttc ttatggatat
      421 accttatgct aatgaaatta tgtggctgtt ttcaacccct gtagttctat ggcttggtaa
      481 ggattttttt atcaatgcat ggaaacaagc aaaacaccgc tctgcaaata tggatacatt
      541 ggtagcactg agtacaggaa ttgcatatct gtttagtgtg ttcaatatgc tatttcctca
      601 attctggcat caaaggggac ttcacgctca cgtttatttt gaagccgctg ctgtaatagt
      661 tgcatttata cttcttggca aaatgttaga agagaaggca aaggggaata catcttcagc
      721 aattaaaaaa ctaatggggt tgcagcctaa aacagttatt gtaattcagt cggatggaac
      781 agaaaagcaa actttcgttg aagatgtgat tgttgatgat atcatacttg tgaaacctgg
      841 cgaaaaaatt gcagttgatg gcaaacttat caatggcagt tcatacgtag atgaaagtac
      901 gctgacagga gagcctgttc ccgttttaaa aaacataaat gacaacgtgt ttgcaggtac
      961 aatcaatcag aaaggcagct ttcagtttaa tgcactaaag gttggaaagg aaaccatgct
     1021 ggcgcaaatc ataaagactg ttcaggatgc gcagggcagt aaggctccgg tacaaaaatt
     1081 agtagataaa attgcaggga tatttgttcc tgtagttata ggcatttcaa tactaacatt
     1141 tgttctttgg caaatttttg gcggtgataa tggattagtg cagggattgt tggcagcagt
     1201 aacggtattg gtaattgctt gtccttgtgc tttaggtctt gcaacaccaa ctgcaattat
     1261 ggttggtgtt ggcaaagcag ctgaaaatgg aattcttata aaagatgcag agagccttga
     1321 acttgcaaag aaaattacaa ctgttgtttt agataaaacc ggtacaatta ctgagggtaa
     1381 accggttgtt acagatgttt attggtataa tgataattca agtactgcat caatcctcta
     1441 tagtattgaa aagaagtctg aacatccatt agcagatgca gtagtaaaga atttcactaa
     1501 gacagaagat atagaactaa cttcatttga aagcattacc ggcaagggtg taagagcaac
     1561 atatcagaaa gagaattatt ttgttggaaa tatgactttc ataaaggagc aaaatatttt
     1621 aatagaagat atgaaagtcg aaaaagctac tgcatggaca aaggaatcaa aaacagttat
     1681 ttggtttgcc gatagtaagc aagtgttgtc cattattgca atttcagaca gtataaagcc
     1741 aacgtcagtg gctgctattc agcaactaca taaaatgggt attgagcttt atatgcttac
     1801 cggtgataac gaatctactg caaaagcaat tgcagaaaag acaggtattg ataaatatca
     1861 ggcaagtatg ttgccacatc aaaaggccga ttttattaag gtgttgcagc aacaaggaaa
     1921 agtagtagca atggttggtg atggaattaa tgatagtaca gcattagcta ctgctgatgt
     1981 tagtattgca atgggaaagg gtagtgatat tgcaatggat gttgcaaaaa tgacaattgt
     2041 ttcctcagat ttaaataaaa ttccaatggc cattcggctg tcaaaacaaa ctgttgcaac
     2101 aatcaggcaa aatttgtttt gggcatttat ttataatgtc attggaattc caattgcagc
     2161 aggtattttg tacccaatca atggatttct tttaaaccca atgattgccg gtgcagctat
     2221 ggctatgagt agtgttagcg ttgtgagtaa tagtttgaga ttaaaatgga aaaagtagca
     2281 gtatatttta caaataaaaa ttataatgtt gtaacctgtc actcaaacaa aataacaaac
     2341 tttgcacaca gaaaagcaat ttattaatac ttaaataaaa tggaaaacaa aaaacatctt
     2401 caattcaaga ccaacattaa ctgtagtggt tgtgttgccg cagtaaaacc acatttagat
     2461 aaactaatca gcagcgaaag ttggcatgta gatacagcta acaaggataa aatattgtct
     2521 gtttcaacaa atgatatttc agaagaagaa attattaata ctgttcagaa ggccggttat
     2581 aaaattgaat tactaaaaaa gtaaaagact tctgaaggaa tttgtgttaa aagtcttgag
     2641 ttattgaaac aatataatga gagttactta aaaaaatacc cgccaaacag tcaaatcaga
     2701 tgtttggcgg gtaaatgtta tcagttaaat tattcaacga taaagtgacc tttcatggta
     2761 ccatagtgac caggaaaaga gcaaaggaaa tcataagttc cttttgatgg agcagtaaat
     2821 tcaatttctg ttgtctcgcc accaccaatt gttttagtat gtgcaattac actactagcc
     2881 atgtcagcag gaatataatc gttgtctttt gctttaattg cagctgttgc gaaatcttgt
     2941 aatgaagtac ctgatgctag caatacaaaa ttgtgtccca ttgcatcttt aggcgatttt
     3001 cctgtgtgct ttaaagttaa cttaacagtt tgtccttctt taacagtgat ttctgttttg
     3061 tcaaatttca tatcatctcc accggatatt tcaactattg caggctcagt tggtggagct
     3121 actgccggtg ctgcttcttc tgttgcagca ggaggtgttg tctctgttgc aggagtttca
     3181 ggttgttttg attgattaca tgctgcaaaa ataaaagcag ctgatgctaa tgctaaaagt
     3241 gattttctca ttttaatgta tttatttgat tgttatatat tgtataattg tttgtaataa
     3301 tatctgaatt aatagcgatt gaactattgt tctaaaaaca ggtgcaaatg tatagcatca
     3361 attagttctt tccaaaatac aagtattaaa ctaccgttaa ttatgaacag tttacagatg
     3421 cgactttatt agaatttaga tatgccgaac attcatttaa aacactcgta attaaataga
     3481 taagggttta attttaatta atttttacga atgaatgatt ttagtcagtt tgaaattttt
     3541 acataaatct gagaattatg ctttctccgt aagcttatct ttaaacttca gtgccaaaaa
     3601 tgtaatgtac gtaattgtca taaaaaaaat accggagatt aaagcaagca cacaggcacc
     3661 ggttgcatat tgcataaaat ttgcatgaat agattgcaat gttatatcag ccatactttg
     3721 aaatttcata tcgtttgaaa cgaagaaacc tccgagataa taactaaaat aaataattaa
     3781 aggaatcatg ggtggaatac tgatgtttgc agcagttata aatagcacct tatttaatcg
     3841 aaacaaaact gctaagggaa tacctacaag caattgaaat ccccagattg gaacaatgcc
     3901 cataaatact ccaaaaccaa cggaaagtga tttttttaaa atgctttcat cacgtctgaa
     3961 aatctcttct tttataattg tgaaaattcc cttccttctg atgtatttga ataatcgtaa
     4021 tggtaaataa taaaaaagtg tcagggttac taaataagta ttcaggatac tgattcttgt
     4081 aaaatcacga aaagggcgaa aatgcgatac ccgttctttc atgtcgtaaa gcactctgat
     4141 atttactgga attattttta catcttgcca tgcaagtttt actatgactt caatttctgt
     4201 ttcaaattta gttgtataaa gtttaatttt ctttaatggt tcaagagggt acaaacgaaa
     4261 tcctgtctgt gtatcgggta atttttgtcc cgtctcaacc caaaaccaaa agttggaaaa
     4321 tttattacca aatgaacttt ttccgggcac tgattcctga ttcatatttc ttgaacccat
     4381 caataatgct tccggaaatt ttagggcatt ctcaatcatt acatcaatgt cttcaggata
     4441 atgttgtccg tcagaatcaa tggtaattgc atttttaaat cctcttgaaa ttgcttcttt
     4501 aaaaccttta cgcaacgaat aacctttgcc ggaatttgtt ttgttgttta gtaaaacaat
     4561 ttgctcatta tattgattaa gaatttctgt tgtgttatct gtcgaaccat cattgataac
     4621 aataacatcg tttcctgagc tgaaatttaa aacaccatca ataactttct ttagcgtttt
     4681 ggaattatta tatgttggta taataacaca tgcagctatg tcagccaatt tattcactga
     4741 taaatggcgg tgattttaaa aaaacaaaca ccctcgtctt cagcagatgt tgctactttg
     4801 tataattgac catctttagt aatactgatt ttaaatgtaa cttcaggatt cttttcagga
     4861 ttcattacat ttaaaaattt gctgttaatg acttgcttca gctttacatt ttgctcaatt
     4921 gcattttgtg tgagttctgt tgtcatttga attaatacca cacctggcgt aaccggttgt
     4981 cccggaaaat gacctttaaa tatttcatgt gcaggattta gtttaatccg atacacacaa
     5041 ttttgcccgg atgttttatt gatgatggtg tagaaatctt tcagcacatc agaagttttt
     5101 aaataatgtg tcgttcagtt ctgtgtttac catttgattt gaaaacttca tgatattcct
     5161 gtcacctgat ttttcataga aagccaattc ttcaagtaat aaattctttt tgttgaatga
     5221 aagttgtatg taatcaaatg cattgctcat tcttttgt
//
LOCUS       DAORQT010000107         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2702_length_5208_cov_5.134873, whole genome shotgun sequence.
ACCESSION   DAORQT010000107 DAORQT010000000
VERSION     DAORQT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_5208_cov_5.134873"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PK134_04975"
     CDS             <1..825
                     /locus_tag="PK134_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRB38234.1"
                     /translation="GLNYEPVFFAYPQVRKLDELVEHITSAAPEYDFVTDDHIGHQFW
                     IVDNDSDINIITEEFAKLKNTYVADGHHRTAAAALVGKERREKNPAHTGKEEYNFFLA
                     VHFPDNQLKIMDYNRVIKDLNGMNSNQFIDKLKENFVVEEKGSDEYRPAALHEFSMYL
                     EGKWYKLNAKPGTFNDKDPIGVLDVTILSDKVLAPMLNITDLRKDKRIDFVGGLRGLG
                     ELKRRVDNGEMKVAFALYPVTMQQLIDIADNNLIMPPKVTWFEPKLRSGLVLHSLD"
     gene            941..2353
                     /locus_tag="PK134_04980"
     CDS             941..2353
                     /locus_tag="PK134_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38235.1"
                     /translation="MMHDDDYEGFDDQSENLGNSVQRFEEMLRNHETYFFDADSLIKI
                     TDFYLEKFDYDKAMEVIKYGQNLHPDNYAFKAKEAYITHLQGNSEEALEMIDQLLTVN
                     PKNEDLYLIKGLIYNALGMFQEGRKTFKDLLSFTEFKEDVYFHVAQTYLQTFDYHGAI
                     HYFKNCLFENKKNEVAADELSECFDRAGRASEGITYFQALIDQDPYNALFWFHLGQMY
                     QCCVEYEKAINAYDYCILIDENMDDAYIQSGQCHMLSGNYEKAIQSFKQSFEYNTPEA
                     FVYDSIAQCYFEMGNHEESRSYFKKAVKSNPSFHSSWYGIGMTLSEEERWYEAIHYLK
                     KAIELNGDISDYWFLLGDCERELGNLEEADEAYRKVIEMDPDFLDIWYEYPEFLMEQN
                     RTDEALQYAYLAVKYFPNDIAVHLRYIILQYRNGNIKAAYSLLSAAAKGEDDLREIIE
                     EIYPELLNDSNIQKILEQNN"
     gene            2359..3144
                     /locus_tag="PK134_04985"
     CDS             2359..3144
                     /locus_tag="PK134_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HRB38236.1"
                     /translation="MNFELSQMPSRSTKPRENGLTMVMDKGLSLRETEDFISSSGEFT
                     DLVKLGFGSSLITPNLDKKLALYKEAGIPVYFGGTLLEAFLIRDEFDKYLKVLSKYKM
                     EYAEISDGSIVIPHKIKCDYIKKLAKHVTVISEVGSKEEGIIIRPNIWIQMMERELEA
                     GVWKVIAEARESGTVGIYRPNGKAHVMLINKIISRVPADKIIWETPQKSQQVYFIKQF
                     GTNVNLGNIAPSEVIALESLRLGLRSDTFFHFLDKESINRFTN"
     gene            3293..4534
                     /locus_tag="PK134_04990"
     CDS             3293..4534
                     /locus_tag="PK134_04990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB38237.1"
                     /translation="MAGFVAKIAIGFHLKDSFLNRGNSKTNLNALACNLYVNGEYSLV
                     QGIPSVDYEPLYPALMSISYAITGYNWLGLTILQAVLHLITSLLLFKIGTRLFHPIAG
                     FFAACYHAFYPYLFTYSFSIYDTTLFVFLLVLTIELLLTEKLSSKRLVLAGIIIGLAF
                     LTRATIITFLPGLFLYVFYRYFKSQNVTAAFISCSLIVAGCLLAMAPWLVRNYSLTGK
                     LLISTHGSFGMWQGNNPLSFEYLKANKSLDEIYRLNPPLPIYAQYPLAARTPDEAVKV
                     SAAYQQEAVNWIKNNFPEFVSMGWLKAEKLWSWNRNPDSKSVKFGSNEGRKSVYLISY
                     LPILLLFPFGLILLFKKHPVEAFTLLLILLAFTGAHSIAIGFTRARIPIDFFLMLCSG
                     ITITWLLQRFFPSLYLKFKSS"
     gene            4531..>4903
                     /locus_tag="PK134_04995"
     CDS             4531..>4903
                     /locus_tag="PK134_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38238.1"
                     /translation="MSDKKYTVIFLGKLPPPYIGPSVACEIILNSKLKDEFNLIHLDL
                     SDKRDINTLAKLDLTNFYLAFKQYFQLFWYCIRYRPDLVYVPAGQTNVGYTRDAGYIL
                     IAKLFGIKVLTHLRGGYFLNWY"
     assembly_gap    4904..4982
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4983..>5208
                     /locus_tag="PK134_05000"
     CDS             <4983..>5208
                     /locus_tag="PK134_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38239.1"
                     /translation="IVLGNNLRKLFNWIVPDEKIFVIPNGGNFPVNYNERNNNKVKVL
                     FLGNYVRTKGVLEALYAAPLVYEKNKNVEFV"
BASE COUNT         1647 a          922 c          949 g         1611 t
ORIGIN      
        1 gggttaaatt atgagcctgt gttttttgca tatcctcaag ttagaaaatt agatgagttg
       61 gttgaacaca ttacctctgc tgcacctgaa tatgattttg taactgacga tcatatcgga
      121 catcagtttt ggattgtgga taatgattcc gacataaaca taatcacaga agaatttgca
      181 aaacttaaaa acacttatgt ggctgacgga catcaccgca cagctgctgc agcattggtt
      241 ggtaaagaaa gacgcgaaaa aaatcctgca cacacaggca aagaagaata caactttttt
      301 ctggcagtgc attttcccga caatcaatta aaaataatgg attacaacag ggtcatcaaa
      361 gacctgaacg gtatgaattc aaatcagttt attgacaaac tgaaagaaaa ttttgttgtt
      421 gaagaaaaag gaagtgacga atatcgtcct gctgccttac atgaattcag catgtatctt
      481 gaaggaaaat ggtataaatt aaatgctaaa ccgggcacct tcaacgacaa agatccaatt
      541 ggtgttttgg acgtaactat tttgtccgac aaagtattag cacctatgtt aaacatcaca
      601 gaccttcgca aagacaaaag aattgatttt gttggaggac ttcgtggtct cggtgaatta
      661 aagagaagag ttgataatgg agagatgaag gtagcctttg cactttatcc tgttacaatg
      721 cagcaactta ttgatattgc tgacaataat cttattatgc caccaaaagt tacctggttt
      781 gagccaaaac ttcgtagtgg cctcgtactg cattcattag actaattttc ttacagtcaa
      841 gtatttaaca acctgctgtt gactgtctgc ctgacaggta ctgcaggttg tttcatattt
      901 tttacctttg acctcaattt gagagaacca atagaagctt atgatgcatg acgatgacta
      961 tgaaggattt gatgatcaaa gcgaaaattt aggtaacagc gttcagcgtt ttgaagaaat
     1021 gctgcgcaat catgaaacct atttttttga tgccgattcg ctgataaaaa ttactgactt
     1081 ttatttagag aagtttgact atgataaggc tatggaagtc ataaaatatg gtcagaacct
     1141 tcaccccgat aattatgctt ttaaagcaaa agaagcatac attactcatt tacaaggcaa
     1201 ctcagaagag gcgcttgaaa tgattgatca gctacttacc gttaacccca agaacgaaga
     1261 cctttatctt ataaaaggtc ttatatataa tgcattgggt atgtttcagg aaggacgtaa
     1321 aactttcaaa gacctgttgt catttacaga gtttaaagaa gatgtttact ttcatgttgc
     1381 acaaacatac ttgcagacat tcgattatca cggtgccata cattatttta aaaattgtct
     1441 tttcgaaaac aaaaaaaacg aagttgctgc tgacgagctg agcgaatgtt tcgaccgtgc
     1501 aggaagagct tcagaaggca tcacctattt tcaggcatta attgatcagg acccttacaa
     1561 tgcattgttc tggtttcacc tgggacaaat gtatcagtgt tgtgttgaat atgaaaaagc
     1621 catcaatgca tacgactatt gtattctgat tgacgaaaac atggacgatg cttacataca
     1681 aagcgggcag tgccacatgt tgtcaggtaa ctacgaaaaa gcgattcagt ctttcaaaca
     1741 aagttttgaa tataacacac ctgaggcatt tgtttatgac agtatagcac agtgttattt
     1801 tgaaatggga aaccatgaag agtcgcgtag ttattttaag aaggctgtaa aaagtaatcc
     1861 atcttttcac agttcatggt atggcattgg catgacattg tcggaagaag aacgctggta
     1921 cgaagctatt cactacctta aaaaagcaat tgaactaaac ggtgatataa gtgattattg
     1981 gtttttactc ggtgattgcg aacgtgaatt aggcaactta gaagaagctg atgaagctta
     2041 tagaaaagtg attgaaatgg accctgattt tttggatatc tggtatgaat atcccgaatt
     2101 tttgatggaa caaaaccgta ctgatgaagc actgcaatat gcatatcttg ccgtaaaata
     2161 ttttccaaat gacattgcag tacatctgcg atacatcatt ttacaataca gaaacggaaa
     2221 cataaaagct gcatatagct tgttgtcggc tgccgctaaa ggcgaagacg atctcaggga
     2281 aattattgaa gaaatttatc ctgaactttt gaatgactct aacattcaga aaattttaga
     2341 acaaaacaat tagtggcaat gaattttgaa ttgagtcaaa tgccttcacg aagtacaaaa
     2401 cctcgtgaga atggcttaac tatggtgatg gataagggcc ttagccttcg tgaaacagaa
     2461 gattttattt cctcctcagg cgaatttacc gatttggtaa aattaggttt cggcagtagt
     2521 ctcattactc cgaatctgga taaaaaactc gctctttata aagaagccgg aataccggtt
     2581 tattttggcg ggactttgtt agaagctttt cttatcagag atgagttcga caagtacctg
     2641 aaagtattat caaaatacaa aatggaatat gctgaaatat cagatggctc catcgtaatt
     2701 ccacataaaa taaaatgcga ttacattaaa aaacttgcta aacatgtaac agtaatttcc
     2761 gaagtaggct caaaagaaga aggaattatt attcgtccta acatttggat tcaaatgatg
     2821 gaacgtgaac ttgaagccgg tgtttggaaa gtaattgcag aagcacgcga aagtggtaca
     2881 gtgggtattt atcgtcctaa tggaaaagca cacgtaatgc ttatcaataa gataatctca
     2941 agagtgcctg ccgataaaat aatctgggaa acaccacaaa aatcgcaaca ggtttatttc
     3001 attaagcagt ttggcaccaa tgtgaattta ggcaacattg ctcccagcga agttattgct
     3061 cttgaatcgt tgcgtttagg gttaagaagt gacacttttt tccattttct tgataaagaa
     3121 tctatcaata gatttaccaa ttaaacttat tgcttttcag gcataatcgt gccttgtaaa
     3181 gttgtacttt tgcagccgcc caaaagggta taatttcata tttccatttc ctttacattc
     3241 attgactgca agtacaaata taaatttaaa aaaactgctg attattattc tattggcagg
     3301 gttcgttgct aagattgcta tcggatttca tcttaaagat tcctttttga atcgtggcaa
     3361 ttcaaaaacc aatcttaatg cacttgcatg taacctttac gttaacggtg aatacagtct
     3421 tgttcaggga attccttctg ttgactatga acctttgtat cctgcattaa tgagtatcag
     3481 ttatgcaatt acaggatata actggttagg tctgaccatc cttcaggctg tattgcatct
     3541 catcacctct cttctgctat ttaaaatcgg caccagactt tttcacccca ttgcaggatt
     3601 ttttgcagca tgttatcatg ctttttatcc atatctgttt acatattcgt tttctatcta
     3661 cgacactaca ctttttgttt ttttacttgt attgactatt gaattattac tcacagaaaa
     3721 actttcatca aaaagacttg tacttgccgg aattataatc ggtcttgcat ttttaacacg
     3781 agcaacaatc ataacttttc ttcccggctt atttctctat gtattctatc gctacttcaa
     3841 atcacaaaat gtaacagcag catttatcag ttgctcatta attgttgcag gatgtctatt
     3901 ggcaatggca ccctggttgg ttagaaatta ttcactaaca ggaaaattat taatcagcac
     3961 acatggttca ttcggtatgt ggcaaggcaa caatcctttg tcatttgaat atctaaaagc
     4021 taacaaatca ctagacgaaa tttatcgttt aaacccaccg ttgccaatat atgcacagta
     4081 tccacttgca gcacgcacac ctgatgaggc cgtaaaagta tctgcagcct atcaacaaga
     4141 agctgtaaat tggataaaaa acaatttccc tgaatttgtt tctatgggtt ggctaaaagc
     4201 agaaaaactc tggagttgga atcgtaaccc tgatagtaaa agcgtgaaat tcggaagcaa
     4261 tgaaggacga aaatctgttt acctgatttc atatctgcct atattactac tctttccatt
     4321 tggattgata ttattgttta aaaaacatcc ggttgaggca tttacgttgt tattgatttt
     4381 acttgccttc accggtgcac attcaattgc tattggattc accagagcac gcatacctat
     4441 tgattttttt ctgatgttat gcagtggcat aaccataacc tggttgctgc aacgcttttt
     4501 tccatctctt tatcttaaat ttaaatcatc atgagtgaca aaaaatatac tgtaattttt
     4561 ctgggcaaac tgcctcctcc ctacattggc ccatctgttg cctgcgaaat tattctcaac
     4621 tcaaaactta aagacgaatt taatctgatt catcttgacc tttctgataa acgtgacatc
     4681 aatacattag caaaactcga tttaacgaat ttttatttag cctttaaaca atattttcag
     4741 ttgttctggt attgtatccg ctatcgccct gatctggttt atgtacctgc aggacaaacc
     4801 aatgttggct atacacgtga tgcaggatat attcttattg caaaactttt tggcattaaa
     4861 gtacttacac atcttcgcgg tggatatttt ctgaactggt atannnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnattgtgct tggcaacaac ctgcgtaaac tatttaactg gattgtacct gacgaaaaaa
     5041 tatttgttat tcccaatggt ggaaattttc cggtaaacta caatgagcga aacaacaata
     5101 aggttaaagt actttttctt ggtaactatg tgcgcaccaa aggagtattg gaagcattgt
     5161 acgcagcacc attagtatat gaaaaaaata aaaacgtaga atttgtat
//
LOCUS       DAORQT010000108         4923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2956_length_4923_cov_5.204191, whole genome shotgun sequence.
ACCESSION   DAORQT010000108 DAORQT010000000
VERSION     DAORQT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2956_length_4923_cov_5.204191"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2474
                     /locus_tag="PK134_05005"
     CDS             <1..2474
                     /locus_tag="PK134_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRB38240.1"
                     /translation="HLLLGLIREGEGMAIKFLKSLNVNLVELRKSVENAVKGSVNNVS
                     NLNNIPLTKQAEKALKITYLEAKIFKSDIIGTEHLLLSILKDEDNIATQILNQYGINY
                     EIMREELETFKSNFKSEAPQSPSDDPYNKDDDEGPGLGSGSTSKKTGDAKSKTPVLDN
                     FGRDLTKAAEEGKLDPIVGRSKEIERVSQILSRRKKNNPILIGEPGVGKSAIAEGLAL
                     RIIQRKVSRVLFNKRIVTLDLASLVAGTKYRGQFEERMKAVMNELEKSPDVILFIDEI
                     HTIIGAGGASGSLDASNMFKPALARGEIQCIGATTLDEYRQYIEKDGALDRRFQKVII
                     DPTSVDETVEILNNIKDRYEEHHHVNYTPEALKACVTLTNRYITDRFLPDKAIDALDE
                     AGSRVHLSNIHVPENIVELEGKISEIKSEKNRVVRSQKYEEAARLRDSEKQLIDQLEA
                     AKKQWEEETKNQRYAVTDEDVAEVVSMMSGIPLQRVAQAETARLIRMPEELKGKVIGQ
                     DEAIKKVVRAIQRNRAGLKDPNKPIGSFIFLGPTGVGKTELAKVLATYLFDTEDALIR
                     IDMSEYMEKFAVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVVLLDEIEKAHPDVFNL
                     LLQTLDDGQLTDSLGRKVDFKNTIIIMTSNIGSRQLKDFGTGVGFATGAKNAQMDEHA
                     RGVIENALKKAFAPEFLNRIDDVVIFNSLSKEDIIKIIDIELEKLYSRIAGLGYKLKL
                     NDEAKDFLADKGNDVNYGARPLKRAIQKYVEDMLAEEIIKAELQEGDNIEVVFDKEAH
                     LLKANISKSSPPPPKSKKKKEENNS"
     gene            3421..>4923
                     /locus_tag="PK134_05010"
     CDS             3421..>4923
                     /locus_tag="PK134_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38241.1"
                     /translation="MKKPILKLIFLIGLTLHPITSFGQWVTISDTNFVNLLQFYFPTC
                     MNGNQMDTTCNGILTATRLYCPHSSISNLDGIQYFKELDTLECHGNPLTTLPNLPDSL
                     KYLACANGPLASLPPLPNGLVYLYCTMGNLSSLPTLPSTLKHLECWENGITSLPPLPN
                     LIVLNCITNPLGSLPPLPATLLALSCANNSLTSLPSLPLGLVSLDCYANQLTSLPAFP
                     LGLVSLACSANQLTSLPALPSSLKNLFCENNQLSNLPALPALDYLHCNNNLLTSLPTL
                     PSTLYGLRCNNNLLSCLPILPPNIGTTSYYFNISNNPISCLPNYINAMNGTGYLNYAL
                     CAEGDSINNPNGCLSFKGVFGKTYSDTNNPCIWDSTDIPIRNIPIHLLDTNGNLYSTF
                     YTQSNGIYNYILDTSTYKVAIDTTNKLYISNCFSPYVDSIFTLTYIAPVAKDINFNIS
                     CKPQFDVGIKSAYTSGRVFPSLPHKLSVSGGDLSHWYNLNCANGVSGTVTI"
BASE COUNT         1619 a          916 c          904 g         1484 t
ORIGIN      
        1 agcatttgtt gctgggtctt atccgtgaag gcgaaggcat ggccataaag tttttaaaat
       61 cgctcaatgt aaacttggtt gaattacgca agtcagttga gaatgccgtt aaaggaagtg
      121 ttaataacgt cagcaacctt aataatattc cacttactaa gcaggcagaa aaagccttaa
      181 aaattaccta tcttgaagct aaaatattta aaagcgatat tatcggcaca gaacatttgt
      241 tgctttcaat tctaaaggac gaagacaata ttgctacaca aatattaaat cagtatggta
      301 tcaactatga aattatgcgc gaagagctag aaaccttcaa gtctaatttc aagtcagaag
      361 caccacaatc gccatctgat gatccctata acaaggacga tgatgaaggt ccggggttgg
      421 gcagtggttc cacatcgaaa aaaaccggtg atgccaaatc aaaaactccc gtactcgata
      481 actttggtcg cgatttgact aaggcagccg aagaaggaaa gttagaccct attgtaggac
      541 gaagcaaaga gattgaaaga gtttcgcaaa ttctgtcacg cagaaagaaa aataatccga
      601 ttcttattgg tgaacccggt gtaggaaaat ctgccattgc cgagggtctt gccttgcgca
      661 tcattcaacg caaggtttcc agagtgcttt tcaataagag aattgtaaca cttgatttag
      721 catcattagt tgcaggaaca aaatatcgcg gtcagtttga agagcggatg aaagcagtga
      781 tgaatgagtt ggaaaaatcg cccgatgtaa ttttgtttat tgatgaaata cataccatca
      841 ttggtgccgg tggtgccagc ggttcgcttg atgcttccaa catgtttaaa cctgcattgg
      901 ctcgtggcga aatacaatgt atcggtgcaa caacattaga tgaataccgt cagtatattg
      961 aaaaagacgg ggccttagac agacgttttc agaaagttat tattgatcct acttctgttg
     1021 acgagacagt agaaattctc aataatatta aagaccgtta tgaggagcat caccatgtaa
     1081 attatactcc ggaggcattg aaagcatgtg taacgcttac taacagatac attaccgatc
     1141 gttttctgcc tgacaaggca atagatgcat tagacgaagc aggttcacga gtgcatcttt
     1201 caaacattca tgtgcctgaa aatattgttg agttggaagg aaaaatttca gagataaaat
     1261 cagagaagaa cagggttgtg cgcagtcaga aatacgaaga ggcagcacgc ctgcgcgatt
     1321 ccgaaaaaca attgatagac caacttgaag cggctaaaaa gcaatgggaa gaggagacaa
     1381 aaaatcaacg ctatgccgta acagatgaag atgttgcaga agtagtttcc atgatgagtg
     1441 gtattccatt gcagcgtgtg gcacaggcag aaacagcacg actcattcgt atgccggaag
     1501 aattaaaagg taaagtgatt ggtcaggatg aagcaattaa aaaagttgtt cgtgccattc
     1561 agcgtaatcg tgcaggatta aaagacccta acaagcctat cggctcattc attttcttag
     1621 gccctacagg tgtgggtaaa acagaattgg ccaaagtact tgccacttat ttattcgata
     1681 ctgaagatgc attaatccgt attgatatga gcgaatacat ggagaaattt gctgtatcgc
     1741 gactcattgg tgcgcctccc ggatatgttg gatatgaaga aggcgggcag cttacagaga
     1801 aggtaagacg caagccatat tctgttgttt tgctcgatga aattgagaaa gcacatccgg
     1861 atgtgttcaa cttattatta caaacattgg atgatggaca gcttaccgac tcattaggtc
     1921 gtaaggttga ttttaaaaac accatcatca tcatgacaag taacattggc tcgcgtcagt
     1981 taaaagattt tggtacggga gtaggttttg ctacgggtgc caagaatgca caaatggacg
     2041 agcatgccag aggtgttata gaaaatgctt taaagaaagc ttttgcacct gaattcctta
     2101 atcgtattga tgatgtagta atatttaatt cgctcagcaa agaagatatt attaaaatta
     2161 ttgatattga attggagaaa ctttattcgc gcattgcagg cttaggttat aaacttaaac
     2221 ttaatgatga ggctaaagat tttcttgcag ataaaggaaa tgatgtaaac tatggtgcac
     2281 gtccgctcaa gcgagcaatt caaaaatatg ttgaagacat gcttgcagag gaaatcataa
     2341 aagcagaatt gcaggaaggt gataacatag aagtggtttt tgacaaggaa gctcatttgc
     2401 taaaggcaaa tatttccaag tcgtcaccac caccaccaaa gtcaaaaaag aaaaaagaag
     2461 agaataattc ttaaaaacaa aaaggctgct ctattgagta gccttttttg cttgggttac
     2521 atacttgtaa tatttaaagc tatcgctttc atgttttttt ctgtacttat atactttcaa
     2581 agtctcccac atttaaaggg aatttgaaat aatagaagca ttgaagggat gatacgtttt
     2641 ttctgaaaat agaaaacatt taattacacc ggacaggttg ctaaggtctt ttcagagtta
     2701 tttaaacaac attttatttt cttcatccac atttttcatc agtgacattt tagaaaattt
     2761 caaccttcta aacttttaaa actttacaac tttcaaactc taaccatacg acctcttgaa
     2821 gaattgcgta aagcaaaagt cgtgagggaa agtaaaaaga aaacaagctg tccgaagtta
     2881 cagcgtgcag cgctgtaata agtttttgtt ttcgtgggca agcagacttt tgtagctttt
     2941 ttcaagcagt cttgattttt tgattctttt gtatcaagac aaaagaatgg aaaagaataa
     3001 aaagaaaaca gcaatttaat ttcacctgtc atttcgcacc gcccctgaca gtgtgattta
     3061 acacaaacaa ttaattttaa tatagcctaa ttaactgcta ttgccttctt aaatcatttt
     3121 tctttttact tttgagctaa tgaatagttt taattccgaa agttatcgga aagactgacg
     3181 ttatacgcaa tccgtgtcgc tccgatgaaa cattctgcaa acttgaaata gtatcactcc
     3241 aaaagccggc aaagccgttt tggctatccc ttctgtaaaa taaaaagaga agtacgtcat
     3301 tttttatttt tttatttggc gtacttcaca ttttatttta ccccaccgca caaataccgt
     3361 tttcaatcta tatatttgtt tccaaattaa ctttagtact tttgacaaac aatatatgta
     3421 atgaaaaaac caatactgaa acttattttt cttatcggat taacattaca tcctataacc
     3481 agttttgggc agtgggtaac aatatccgat accaattttg taaacctact tcaattttat
     3541 tttccaacct gcatgaatgg aaatcaaatg gacaccacat gcaatggcat tcttactgca
     3601 actagattgt attgtcctca tagttcaatt agcaatttag acggcattca atatttcaaa
     3661 gaattagata ctttggaatg tcacggtaat ccacttacta ctttaccaaa tctccctgac
     3721 tccttaaaat acttagcgtg tgctaatggc ccacttgcat cacttccacc tttacctaat
     3781 ggcttagttt atttatattg tacaatgggg aatctttctt ctttaccaac tctaccttca
     3841 acattaaaac atttagaatg ctgggaaaat ggaataacaa gccttccccc acttcctaac
     3901 ttaatagttt taaattgtat tactaatccc ctcggttcat taccaccatt acccgccacc
     3961 ttgttggctt tatcctgtgc aaataattca ttaacttctc ttccatcgct tccattagga
     4021 ttagtctcac tggactgtta tgcaaaccaa ttaacaagtt tacctgcctt tccattagga
     4081 ttagtctcac tggcctgttc tgcaaaccaa ttaacaagtt tacctgcctt accttcctca
     4141 ttaaaaaatt tattttgcga gaataatcaa ctaagcaatc tcccagcact gccagcattg
     4201 gattatttac attgtaataa taacttactt acaagtctgc caacacttcc aagtacattg
     4261 tatggattaa ggtgtaacaa caatctgcta tcatgtcttc caattttacc ccccaatatt
     4321 ggtactacaa gttattactt caacatatcc aataacccga tttcctgttt gccgaattac
     4381 ataaatgcga tgaacggaac aggctattta aattatgcgc tgtgtgctga aggagattct
     4441 attaataatc cgaatggatg cttaagtttt aaaggtgtgt tcggaaagac ttattctgat
     4501 acaaacaatc cttgcatttg ggatagcacc gatattccta ttagaaatat acctattcat
     4561 ttacttgata caaatggaaa tttgtattcc acattttata cacaaagcaa tgggatttac
     4621 aattatattt tagatacatc aacctataaa gttgcaattg atacaacgaa taaactttat
     4681 atttccaatt gcttttctcc ttatgtagat tctattttca cactaacata cattgctcca
     4741 gttgccaaag acataaattt taatatcagt tgcaaaccac agtttgatgt aggaataaaa
     4801 agcgcatata cgagcggaag agtttttcct tcactaccac acaagttatc agtttcggga
     4861 ggagatttaa gccattggta caatttaaat tgcgccaatg gagtaagcgg cactgtaaca
     4921 att
//
LOCUS       DAORQT010000109         4903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_2973_length_4903_cov_4.896452, whole genome shotgun sequence.
ACCESSION   DAORQT010000109 DAORQT010000000
VERSION     DAORQT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2973_length_4903_cov_4.896452"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK134_05015"
     CDS             <1..204
                     /locus_tag="PK134_05015"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280717.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRB38242.1"
                     /translation="RSDINSERKNISGGTDAARLSFMGMPSPNIFTGEMGNHSKEEYV
                     SVQDMNKAVEVLVNLVQVWEQRA"
     gene            448..2508
                     /locus_tag="PK134_05020"
     CDS             448..2508
                     /locus_tag="PK134_05020"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRB38243.1"
                     /translation="MKKQITLLFGIMCFALAVNAQYYYFPHINAGQNPGGLNTDDEYP
                     VAGGQPAGWTSIHPGSATTPQWSTTVTIPFAFSFNGAAVTNYKVSTTGVLTFTTGAAT
                     PPPVANVALPSALVPDNSVCCWGISGSGTNDNICTKTFGTAPNRQHWISFTSYTDPGN
                     TAYTYYSIVLEESTNKIYVVDQRNGGTALTLTIGVQINATTAVSVAGSPNISQYAGAD
                     PSPADNSYWEFIYGSQPANDVALSSLNYTQYVTTPTNVTLSGVITNLGSSAITTVDLK
                     YQLNGTTYTDTKTGLNIAPLGTYNFTHATPINASAVGTYSFNFWADLAGDANHINDSL
                     NGVINYLPFLPTKRIVFEEATGTWCGWCPRGAVYMDSLRNLHGQEVMLIAVHNGDPMT
                     VAAYDSWMGTKISGYPSGLVDRKDLDVDPSDFITEYNSLISDIPPCDVNVTATYNSST
                     NVGTFTVSPQFAASLNGDYRINCVVTEDNLTGTTSSWSQANYYSYQTANMPLQGAGHD
                     WQNETDPVPAANMEYDHVARAILGGNDGVQGSLPANITSGGTYSWTFNYNIPATQNVS
                     NLIIIGWVSDFATGRILNANSATVTTGIHNLTGTYFNMNVTPNPITSDYGQVKVNLKK
                     NADVVFEIIDVLGNIVRTNYQSQMTAGEYIYPVNFTGLSNGIYTVRMNASGETLTQKV
                     VLNK"
     gene            complement(2656..3738)
                     /gene="aroC"
                     /locus_tag="PK134_05025"
     CDS             complement(2656..3738)
                     /gene="aroC"
                     /locus_tag="PK134_05025"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRB38244.1"
                     /translation="MAGNTFGTLFRITTFGESHGPLIGVVIDGCYSGVQLDLELIRTQ
                     LSRRKPGQSAITTQRMEDDEPEIVSGVFNGITTGAPLTILIRNKDQRPTDYDALKDAY
                     RPSHADYTYEKKYGIRDHRGSGRASARETAARVAAGAVAIQMLNAANISIDAYVSRIG
                     NVSIPENVSIDTTQTYNNILRCPHEATAQLMQKSIEETAQTGDTLGGIISCIISGVPA
                     GLGEPVFDKFHAQLGKAMLSINAVKGFEIGSGFNGTSQMGSQQNDVFEIDKDGKVFTR
                     TNNSGGVQGGITNGMDITFNVAFKPVSTLMKSQQSINLQNEKVVIQPGGRHDVCVVPR
                     ATPIVEAMAAITTLDFLMIANAAKCK"
     gene            complement(3740..4219)
                     /locus_tag="PK134_05030"
     CDS             complement(3740..4219)
                     /locus_tag="PK134_05030"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB38245.1"
                     /translation="MHIRQASTDDCSAISALANSIWHSHYPGIISMQQIDYMLSKMYS
                     VEKLNHLLESGSVILVAEEDEKLLGYICFREETDYSFFLDKLYVDSELQHKNLGSLLL
                     EAMLSSLPEDIIIRLQVNRSNYKAINFYFKKGFVIERAEDFDIGNGFFMNDFVMIKK"
     gene            complement(4223..4786)
                     /locus_tag="PK134_05035"
     CDS             complement(4223..4786)
                     /locus_tag="PK134_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRB38246.1"
                     /translation="MKILVIDNYDSFTYNLVHYIEKAAGVQPTVWRNNSHSVDEVAAF
                     DKICISPGPGVPSQAGFTKEVIKRFTSEKSILGVCLGHQAIAEVFGGSLRNLQQVLHG
                     IVRPVNVLVQDVLFENMPSSFPAARYHSWVVDQTGSQLQVIAGDDIGEVMAIKHIQYP
                     VYGVQFHPESIMTEHGLQLIYNWIYKT"
BASE COUNT         1564 a          974 c          861 g         1504 t
ORIGIN      
        1 cgcagtgaca tcaattctga acgcaaaaac atcagcggcg gaacagatgc agcacgatta
       61 tcatttatgg gcatgcctag tccgaatatt tttacaggag aaatgggtaa tcacagtaaa
      121 gaagaatatg taagtgttca ggatatgaat aaagctgttg aggtgttggt taatttagtt
      181 caagtttggg aacaacgagc ctgacagtag tcccttcctc ttattcagtt cgataaattt
      241 actaaagaaa tttaagcttg gtttgaacta attagttttc acttagttgg tattttaatg
      301 caatcgtatc agtaatacaa actgttatag actaacttta aacaaccgtc cattgccttc
      361 gtagagctaa aacaattatt taactaaaag tcttaatttt acgttgcgaa aaatgataat
      421 ttcgcagtca attaaattta aataaaaatg aaaaaacaaa ttactcttct atttggcata
      481 atgtgttttg cattagctgt aaatgcacag tattattatt ttccacacat caatgctggg
      541 caaaatccgg gtggccttaa cacagatgat gaatatcctg ttgctggagg ccaacctgca
      601 ggttggacat ccattcatcc cggaagtgct accactccac aatggtcaac aactgtaaca
      661 attccgtttg cattttcttt taacggtgcg gcagttacaa attacaaagt ttccacaaca
      721 ggagttttaa cctttacaac aggtgcagct acacctcctc cggttgccaa cgttgcatta
      781 ccttcagctt tagtacctga taattcagtt tgttgttggg gaatttcagg aagtggtaca
      841 aatgataata tctgtactaa aactttcgga acagcaccaa accgtcagca ctggatttca
      901 ttcacttcct atactgatcc gggcaacact gcatatactt attatagtat tgtattagaa
      961 gaatcaacaa ataaaattta tgtagttgat caaagaaacg gtggaacagc gttaacactc
     1021 acaataggtg ttcagattaa cgccactaca gccgtttctg ttgcaggttc tccaaacatt
     1081 tcacaatatg caggtgcaga tccatcacct gctgacaaca gttattggga atttatctat
     1141 ggttcacagc cagcaaatga tgtagcatta tcttctttaa actatacaca atatgttaca
     1201 acaccaacta acgttacatt atcaggtgtt attacaaatc ttggttcaag tgcaataact
     1261 acagttgatc ttaaatacca actcaatggc acaacttata ccgacacaaa gacaggatta
     1321 aatattgcac cccttggaac atataatttt actcatgcaa ctcctataaa tgcaagtgca
     1381 gtaggaactt attctttcaa cttttgggca gatttagccg gagatgcaaa ccatattaat
     1441 gatagtttaa atggtgttat taactatctt ccgtttttac caacaaaacg tatagtattt
     1501 gaagaagcca caggtacttg gtgcggatgg tgtccacgtg gtgctgttta catggattcg
     1561 ctccgcaact tacatggcca ggaagttatg ttgattgctg ttcacaatgg cgatcctatg
     1621 actgttgccg cttatgattc atggatggga actaaaatca gtggctatcc aagcggattg
     1681 gttgatcgta aagatttaga tgttgaccca tcagatttta tcactgaata caacagcctt
     1741 atttccgata ttcctccttg tgacgttaat gtgactgcaa cgtataattc aagtaccaat
     1801 gttggaacgt ttactgtaag cccacaattt gcagcatcat tgaatggcga ttatagaatt
     1861 aactgtgttg ttacagaaga taatttaacc ggcacaactt catcatggag tcaagcaaac
     1921 tattatagct atcaaacggc aaatatgccg cttcagggtg ccggacatga ttggcagaat
     1981 gaaacagatc ctgtaccagc agccaacatg gaatatgatc acgttgctcg tgcaattctt
     2041 ggtggaaatg atggtgtgca aggttcattg cctgcaaaca taacttccgg tggtacttac
     2101 agttggacat ttaattataa tattccggca actcaaaatg taagcaatct aatcatcatt
     2161 ggatgggttt ctgattttgc taccggtaga atcttaaatg caaacagtgc aacagttact
     2221 acaggaattc ataacttaac aggaacatat ttcaatatga atgtaacacc aaatccgata
     2281 acatctgatt atggtcaggt gaaagttaat ttgaagaaaa atgcagatgt agtattcgaa
     2341 attattgatg tattaggtaa tatcgttcgc actaattatc aatcacagat gacagcaggt
     2401 gaatatattt atcctgtaaa tttcacaggt ttaagcaatg gtatttatac cgttagaatg
     2461 aatgcatccg gtgaaacatt aacacaaaaa gtagtattga ataaataaaa ttatatcggt
     2521 agatttaccg tataagatta aaatcgaaaa ggcagtttca atataaaaat tgaaactgcc
     2581 tttttctttg ctagtcaata atctttatga gtaaacctta atacctgaat accgtaaaca
     2641 ttaaaattat atcaactatt tgcacttagc agcattggca atcattaaaa aatcgagtgt
     2701 tgtaattgct gccattgcct ctacaattgg tgttgcacgt ggcacaacac aaacatcatg
     2761 acgtcctccg ggttgaataa ccactttttc gttttgtaag ttaatgcttt gttgtgactt
     2821 catgagtgtt gacactggct taaatgctac gttaaaagta atgtccatac cattggtaat
     2881 tccaccctgc actcctccgg agttgttggt cctagtaaaa actttcccat ccttgtcaat
     2941 ctcaaaaaca tcattctgct gtgaccccat ttgcgaagtt ccgttaaacc ccgaccctat
     3001 ttcaaagcct ttaacagcat taatactaag catggcctta cctagttgtg catgaaattt
     3061 atcaaataca ggttcaccta acccggcagg aacaccacta ataatacagg atattattcc
     3121 tcctaaagtg tctcctgtct gtgcagtttc ttcaatactt ttttgcatca actgtgcagt
     3181 ggcttcatga ggacagcgca aaatattatt ataggtttgt gtagtatcaa ttgaaacatt
     3241 ttcaggaata gaaacattgc cgatacggct tacataagca tcaatagaaa tatttgcagc
     3301 atttagcatt tgtatagcaa cagctcctgc agccacacgt gcagcggttt cgcgtgctga
     3361 tgctcttcca cttccgcggt gatcacgaat accatatttt ttctcatagg tataatctgc
     3421 atgtgatggc ctatacgcat ctttaagtgc atcataatct gtaggacgtt gatctttgtt
     3481 acgaataaga atcgttaatg gtgcacctgt tgttatccca ttaaaaacac ctgaaacaat
     3541 ttccggctca tcatcttcca tccgctgcgt agtaattgca gattgacccg gcttgcgtct
     3601 tgacagttgt gttcttatca attctaaatc caactgaact cccgaataac aaccgtcaat
     3661 aacaacacca ataaggggtc cgtgtgattc accaaaagtg gttattctga aaagtgttcc
     3721 gaatgtatta cctgccatat tactttttaa tcattacaaa atcattcata aaaaagccat
     3781 tgcctatatc aaagtcttct gcacgttcaa taacaaaacc ctttttaaaa taaaagttaa
     3841 ttgctttata atttgaacgg ttaacctgca aacgtattat aatatcttca ggcaaactac
     3901 tcaacatagc ttcaagaagc aatgaaccca aatttttatg ttgcaattct gaatcaacat
     3961 ataatttatc gaggaaaaaa ctgtaatcag tttcttcacg aaaacaaata tagcctaaaa
     4021 gtttttcatc ttcttctgca actaaaatta cagaacctga ctcaagcaga tggttcaatt
     4081 tctcgacact gtacattttg ctcaacatgt aatcaatctg ttgcatagag ataataccag
     4141 ggtaatgact atgccaaatg ctatttgcta atgcgctgat ggcactacaa tcgtctgttg
     4201 aagcttgcct gatatgcatg gttcaggttt tataaatcca attatatata agttgcagtc
     4261 catgttcagt cataatgctt tccggatgaa attgaactcc ataaacagga tattgtatat
     4321 gttttatggc catcacttcg ccaatgtcgt caccggcaat tacctgaagt tgactgcctg
     4381 tttgatccac aacccatgaa tgataacgtg cagccggaaa tgatgaaggc atattttcaa
     4441 acaacacatc ttgtaccaat acattcaccg ggcgtactat accatgcaaa acttgttgta
     4501 aattacgaag actgccacca aaaacttctg caattgcctg atgacctaaa caaacaccaa
     4561 gtattgattt ttctgatgta aatcgtttga taacttcttt ggtaaaacct gcctgagaag
     4621 gcactcccgg ccccggagaa atacaaatct tatcaaatgc agcaacctca tcaacagagt
     4681 gagaattgtt tcgccaaact gttggttgca cacctgctgc cttttcaata tagtggacaa
     4741 ggttgtaggt aaaagaatcg tagttatcta tgactaaaat tttcactcgt caaaaatagc
     4801 gaatgtttta tggcacgaca ttcaatcctg atcggtaaat ttatacccaa cacctcttac
     4861 agagataaaa tacttagggt ttgactggtc cggttcaaaa tat
//
LOCUS       DAORQT010000110         4870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3002_length_4870_cov_10.023468, whole genome shotgun sequence.
ACCESSION   DAORQT010000110 DAORQT010000000
VERSION     DAORQT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_4870_cov_10.023468"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK134_05040"
     CDS             <1..729
                     /locus_tag="PK134_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRB38247.1"
                     /translation="RVRKAFIARDSEHILLSADYSQIELRLAAEISGDENMLEAFRQG
                     LDIHQATAARVYNVALADVTKEMRSKAKMVNFGIIYAISAFGLSQRLGIPRKEAAELI
                     ENYFIQYPKLRNYMSETLEFARHNGYVKTLMGRRRYLKDINSRNFTVRGFAEREAINS
                     PLQGSAADLVKLAMIQIHEEFKRQNLKTKMTLQVHDELVFDAHQDEVDLIKPIIREKM
                     IHAIKTQVPLEVEIGIGSNWLDAH"
     gene            786..>3017
                     /locus_tag="PK134_05045"
     CDS             786..>3017
                     /locus_tag="PK134_05045"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRB38248.1"
                     /translation="MKILNYLCTAFILLLGQSTQAQLTVTSGNPAAAIVDTLVGTGVS
                     VANVTYTGNANSKGTFRCTGGCNLGITRGVFLSTGNTGQMPTSPPSDFHSSDMSGSGD
                     AQLNLIVSPNLTQDAAVLEFDFTPAADSVGFKFVFASEEYNDYVNTAYNDVFAFYISG
                     PGIPGSPKNLALIPGTSIPVSINNVNNGNSAGAATGPCNHCANFVDNVPNGGTANFFD
                     GFTTVISAGIKVMPCQTYHMKFAIADVNDGVFDSGVFLEAKSFSSIGNVGIAANGVQI
                     IQNLDTVYACDGDSVVLTASPAGSYNWNNGQTTQTIVVTQANITPSGVYQYAVLNSSP
                     ACFASSKQVKVIFVTPTATITPLGSTALCPGGSVVLQANPGSSYLWSNGATSDSIVVS
                     AAGTYTVTVTQFGNCYAVSTPVVVTQSAPVAAINGVLTICSGSNTTLTATAGASYLWS
                     TGATTQSVSVGTSNTYTVTVTYSGGCTATASVNVTANTNPVPSITGNNSICQGQSSTI
                     NAGSYAGYLWSTGATAATINVNTAGNYSVTVTDANGCTGTASFNLTVNANPTPTITGV
                     TSFCAGENSTLNAGGGYTNYLWSGGATTQTINVTAGGNYAVTVTNAAGCTATTNKVIT
                     VNALPVPSVTGTLSFCAGKNTTLTVPGSYTTYQWSNGATTSNITVSTASTFTVTVTDA
                     NGCTGSKSATTTINTNPVPSIIGNSSICQGQSSTINAGSYAGYSWSTGATAATINVNT
                     AGNY"
     assembly_gap    3018..3117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3118..>4870
                     /locus_tag="PK134_05050"
     CDS             <3118..>4870
                     /locus_tag="PK134_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38249.1"
                     /translation="GCTATTNKVITVNALPVPAISGPAGICPGASANLFAGSYASYLW
                     SNGNTTPSITTNVSGNYTVTVTDANGCVGSSNYNLLAFQNPIPVISGTAAICQNQNSV
                     LDAGSFASWQWSTGAVSQTISVTQSGTYSVTVTNTDGCVASTSFNVVVNPLPVPIISG
                     TSAFCSGNSSVLTTGGGSFTSYQWNTSATSNAITVTAQGNYVVTVTDANGCTASTNQA
                     VTVWNLPTPVITGNNSICSGTQTTLSAGNYSAYQWSTGATTDSIAVSNGANYIVTVTD
                     VNGCSGTSAPFALTINQLPTAIISGSTAICIGQSTSLQIQFTGTAPYSYRYSDGTSVS
                     AILNTNNSAAQILVTPGTTTDYTLTQINDANCTGNVSGVARVVVNPLPQPVISGSAGE
                     CDGITATLTTGNFAAYLWSTGATSQSIAVNSSGSYVCTVTSTAGCKASAIHNFEAYPV
                     PVVSFTNDSSLTCELPIINFTNTSQYQLGSVFEWNIDNTIYNSLNATHQFSGSGSYTV
                     SLTITSPKNCVGEAIRNVNITVFPLPQASFTIAPDQAPLFNSEVAFGDASANAVSWLW
                     DFGDGDTSTLQNVSHFYA"
BASE COUNT         1488 a          922 c          940 g         1420 t
ORIGIN      
        1 cgtgtgcgta aagcatttat tgcacgtgac agcgaacata tattgttgtc agcagattat
       61 tcacagattg aattaagatt agcagcagaa ataagtggag atgagaatat gcttgaagca
      121 ttcaggcagg gacttgacat acatcaggca acagcggcac gtgtttataa tgttgcatta
      181 gctgacgtta ctaaagagat gcgtagcaaa gcaaagatgg tcaacttcgg aattatctat
      241 gctatttctg cttttggttt atcgcaacgt ttgggaattc cacgaaagga agctgctgaa
      301 ttgatagaga attattttat tcagtaccct aagcttcgta actatatgag cgagacatta
      361 gagtttgccc gtcataatgg ttatgttaaa acacttatgg gcagaagacg ttatctgaag
      421 gatataaatt caagaaattt tactgttcgt ggatttgccg aacgcgaagc aattaactca
      481 cccttacagg gtagtgctgc cgatttggtt aagctggcaa tgattcaaat tcatgaggaa
      541 tttaagagac aaaatcttaa aactaaaatg acgcttcagg tgcatgacga actagttttt
      601 gatgctcatc aagatgaggt tgatttaata aagcctatta tcagagaaaa gatgattcat
      661 gccattaaaa cccaagttcc tttagaggta gaaattggaa ttggctctaa ttggctggat
      721 gcgcattaaa tacttgcatg attaaataaa atatcatact tttggtttgc caaaaagcca
      781 aaaaaatgaa aattttaaac tatctgtgca ctgcctttat tttattacta ggacaaagca
      841 cgcaagcgca attaactgta acatcaggaa atcctgcagc agctattgtt gatactcttg
      901 taggaacagg tgtttcagta gcaaatgtta cctataccgg taatgcaaat tctaaaggta
      961 ctttcaggtg tacaggtgga tgtaatttag gaattacaag aggcgtgttt ctctcaaccg
     1021 gaaatacggg tcaaatgcct acatcaccac catcagattt tcatagtagt gatatgagtg
     1081 gttcaggtga tgcacaatta aatttaattg tatcgcccaa cctgacacag gatgctgctg
     1141 tgcttgaatt tgattttact cctgcagcag acagtgtagg atttaagttt gtttttgctt
     1201 ctgaggagta taacgactat gtgaacacag catataatga tgtttttgct ttctatattt
     1261 caggaccggg cataccaggt agtcctaaaa acttagcctt gattccgggt acttcaattc
     1321 ctgtttcaat aaataatgta aataatggca atagtgccgg agcagctacc gggccatgta
     1381 atcattgtgc aaactttgta gataatgttc caaatggagg tactgccaac ttttttgatg
     1441 gtttcacaac tgtaatatca gcaggaataa aagtaatgcc atgtcagacc tatcacatga
     1501 aatttgcaat tgcagatgta aatgatggtg tttttgattc tggagtcttt ttagaagcaa
     1561 aaagtttttc aagtattggc aatgtaggaa ttgcagctaa tggagttcaa atcatacaaa
     1621 acctcgatac agtttatgcc tgcgatggcg attctgttgt gttgactgca tctccagccg
     1681 gcagttataa ttggaacaat ggtcaaacta cacaaactat tgttgttaca caggctaata
     1741 ttacaccatc aggtgtttat caatatgcag tattaaatag ttcgcctgca tgttttgcat
     1801 ctagtaaaca ggtaaaagta atttttgtaa caccaacagc caccatcaca ccattaggtt
     1861 ctactgcatt atgtcccgga ggttcagttg ttttacaagc taatcccgga agtagttact
     1921 tgtggagtaa tggggcaact tcagattcta ttgtagtatc agcagccgga acatatactg
     1981 tcaccgtcac tcagtttggt aattgctatg ctgtttcaac acctgttgta gttacacagt
     2041 cagcacctgt tgctgctatt aatggagtgt taactatttg ctccggaagt aataccacac
     2101 ttacagcaac agctggtgct tcttatctat ggtcaaccgg tgcaactaca caatctgttt
     2161 ctgttggtac aagtaataca tatactgtta ctgtaaccta ttcaggtgga tgtactgcaa
     2221 ctgcatctgt taatgttact gccaacacaa atcctgttcc atccatcaca ggcaacaaca
     2281 gtatttgtca gggacagagc agtacaatca atgccggaag ttatgcagga tatttatggt
     2341 caacaggtgc aactgcagca acaattaatg taaacactgc aggaaattat tctgtaacag
     2401 ttactgatgc caatggttgc acgggaactg cgtcatttaa tcttacagtt aatgccaacc
     2461 caacaccaac tatcaccggt gtgacttcat tttgtgcagg agaaaattca acactcaatg
     2521 caggcggggg atacaccaac tatttgtgga gtggaggagc aactacacaa actatcaatg
     2581 tcaccgctgg tggcaactac gcagtaaccg ttacaaatgc agcgggatgt actgcaacaa
     2641 caaataaagt tattactgtt aatgcattac ctgtgccatc tgtaacaggt acattatcat
     2701 tctgtgcagg taagaacact acacttacag tacccggaag ttatactaca tatcaatggt
     2761 caaatggagc aacaacatct aatattacag tcagcacagc ttcaactttt acagtaacag
     2821 taaccgatgc caatggttgt acaggaagta aatctgcaac aacaactatc aacacaaacc
     2881 ctgttccatc catcataggt aacagtagta tttgtcaggg gcaaagcagt acaatcaatg
     2941 caggtagcta tgcaggatat tcatggtcaa caggtgcaac tgcagcaaca attaatgtaa
     3001 acactgcagg aaattatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
     3121 gatgtactgc aacaacaaat aaagttatta ctgttaatgc attacctgtg ccggccataa
     3181 gtggtccggc tggtatttgc cccggtgcta gtgccaatct ttttgcagga agttatgctt
     3241 cttatctatg gtcaaatgga aatacaacgc catcaattac tactaatgtt tccggaaact
     3301 acactgtaac agttacagat gcgaacggat gtgtaggctc atccaattat aacctacttg
     3361 ctttccaaaa tcctattcct gttatcagtg gaacagcagc aatttgccag aatcaaaatt
     3421 cagtattaga tgcaggtagt tttgcctcat ggcaatggtc aacaggtgct gtatcacaaa
     3481 ctatatcagt aactcagtca ggaacttatt ctgttacggt aaccaataca gatggttgtg
     3541 ttgcatcaac ttcgtttaat gtcgttgtga atccacttcc tgttcctata atttcaggta
     3601 cttctgcatt ttgttcaggt aattcctcag tgctgactac tggcggaggt agttttactt
     3661 cgtatcaatg gaatacttct gcaacatcta atgcaataac agtaacagct caaggaaatt
     3721 atgttgtgac cgttactgat gctaatggat gtacagcttc aacaaatcag gcagttacag
     3781 tgtggaatct tccaacgccc gtaatcaccg gaaataattc aatctgcagt ggcacacaaa
     3841 ctactttatc agctggaaat tattctgcat atcaatggtc aacaggagca actacagatt
     3901 caattgctgt ttcaaatggt gcaaactata ttgtaacagt aacagatgtt aatggatgtt
     3961 caggaacatc agcaccattt gcattgacga taaatcaatt acctactgca ataatttcag
     4021 gttcaacagc catttgtatt ggtcaatcta catctttaca gatacagttt accggaactg
     4081 caccttatag ttatcgttat agtgatggca caagtgtatc agcaatttta aatactaata
     4141 attcagcagc acaaatttta gtgactcccg gtactacaac agattatacg cttacacaaa
     4201 taaatgatgc aaattgtaca ggtaatgttt caggagttgc aagagtagta gttaatcctt
     4261 taccacaacc tgtgatttca ggttctgcag gtgagtgcga tggcataact gccacactta
     4321 ctacaggaaa ttttgcagca tatttatggt caacaggagc aacttcgcaa tctatagcag
     4381 ttaattcttc cggaagctac gtatgtacag taacttccac agcaggatgt aaagcttctg
     4441 caattcataa ttttgaggct tatccagtac ctgtagtttc ttttacaaac gacagcagcc
     4501 tgacttgtga actgccaatt attaatttca ccaacacttc gcaatatcag ttaggatcag
     4561 tttttgaatg gaatattgat aatacaattt ataattctct gaatgctaca catcagtttt
     4621 caggttcagg aagttatacc gtatcattaa ccataacctc gccaaagaat tgtgtaggcg
     4681 aagctatcag aaatgtaaac atcacagtat ttcctttgcc acaagcatca tttacaatag
     4741 cacccgatca ggcaccatta tttaattccg aagtagcatt tggagatgcc tctgcaaatg
     4801 ctgttagctg gttgtgggat tttggagatg gcgatacatc tacattgcaa aatgtaagtc
     4861 acttttatgc
//
LOCUS       DAORQT010000111         4782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3087_length_4782_cov_6.771737, whole genome shotgun sequence.
ACCESSION   DAORQT010000111 DAORQT010000000
VERSION     DAORQT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3087_length_4782_cov_6.771737"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK134_05055"
     CDS             <1..458
                     /locus_tag="PK134_05055"
                     /inference="COORDINATES: protein motif:HMM:NF027450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HRB38250.1"
                     /translation="ELIEYLQQTDKPQLTYLMARVKQETVVPDIKLLEFRKQRFNERV
                     NAMLHYADSLTSCRSMMLLNYFGETTNTRCGTCDYCRKRNKIELNDLTFEKQNAEIKD
                     FIQQQTLRYDELLEKFSASKKEQYVQSLQWLIDSGKIIISESDILLWHE"
     gene            451..1041
                     /locus_tag="PK134_05060"
     CDS             451..1041
                     /locus_tag="PK134_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRB38251.1"
                     /translation="MSKLLQSFYLQESAVKVARQLLGKELCTKINGKLTSGIITETEA
                     YMGVEDKASHAWNNRRTQRTEIMYRQGGVAYVYLCYGIHSLFNVVTNIQDVPHAVLIR
                     SIFPKRGFELIKKRRPVNKPLKQIASGPGMVSQALGIDCSMTGTSLLGNKIWIENNDL
                     QLTEKQITTTVRIGVDYAGNDAMLPYRFLINPEILN"
     gene            1041..1751
                     /gene="mtgA"
                     /locus_tag="PK134_05065"
     CDS             1041..1751
                     /gene="mtgA"
                     /locus_tag="PK134_05065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRB38252.1"
                     /translation="MKNIFIRIRKILWKIIKWFLILSIAGVILFRFVPIPFTPLMIIR
                     FVEQVFDSERDIQFKKDWEPISNISPLLVKAVITSEDQRFNEHFGLDMEAIQKAAQYN
                     EKHKNKKRGASTISQQTAKNVFLFPSRTWLRKGFEVYFTFLIEIFWSKERIMEVYLNV
                     IELGNGVYGAEAASEYYFNKPASQLSVHQAASLAAILPLPLKWSPVKPNARVVRRTEW
                     IKRYMKYAEGLESVNGEK"
     gene            complement(1939..2373)
                     /locus_tag="PK134_05070"
     CDS             complement(1939..2373)
                     /locus_tag="PK134_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38253.1"
                     /translation="MLTFILPAICFVTEHFFTNTVLSFNLFGKWFIFSAVGLRLFLAG
                     TKQVINPEFTAKEIFHLDSSDSFPILRELGFANLCFGLVAVVSIFKPEWRTVSAFASG
                     LYFGFAAIQHGFNKTSGINEKFALWTDLIIFVFLSVYFIKSI"
     gene            complement(2545..3021)
                     /locus_tag="PK134_05075"
     CDS             complement(2545..3021)
                     /locus_tag="PK134_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB38254.1"
                     /translation="MMTNISGSYFLNMFIIKIIESRKEDINKLQDIFLETRRSTFSWV
                     DTSQFKLSDFEKETEGEYVLVAMIDEKVIGFISVWVADNFIHHLYVDKSFQDQKVGTK
                     LLNAVLDKFGLPVRLKCEIKNQKAVSFYRQKGFVEIEQGHSEMGVYILFELRKKLS"
     gene            complement(3073..3969)
                     /locus_tag="PK134_05080"
     CDS             complement(3073..3969)
                     /locus_tag="PK134_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38255.1"
                     /translation="MMQKIQIFIVIFFLSSCFTFGQDHDSLFARLNGISNNGIDFFNV
                     DGIEITTQKVPADLTPKSISKKFKNLAIKEKEIISTDSTLGFSNFYVSKSKTESGITI
                     NMNYYFFKNSDDKVIGISFIAMNKTDRELEKEFVQLVYDNAIPESVYATLNIGDINFA
                     GRKIPLGSGCRWMGINNVQCPSWGQMNWSVHKTLEDASLSVNNQIAFIKAGNKGKIVS
                     DTTVAVIFEGNETNARKIIYDFKGATSVLVGMSGGKTLTVYFVATPVRNNFVSCVMSF
                     WNNDQINPSGLPPLLEQVMKLK"
     gene            complement(3966..4331)
                     /locus_tag="PK134_05085"
     CDS             complement(3966..4331)
                     /locus_tag="PK134_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38256.1"
                     /translation="MRNLVIIILVLFSVSCMTNYPLTTFYVKNTTDKTLNFKASIIKQ
                     SQMGPYEMTLPFMVFPNDSVVARRTNFKKDASPTAWFKKFIIFQTDSLSFNDPKNKDN
                     WIKTTDEKGRPVYTFTMTK"
BASE COUNT         1637 a          808 c          840 g         1497 t
ORIGIN      
        1 atgagttgat agaatatcta caacagacag ataagcctca gctaacctat ttaatggcaa
       61 gggtgaaaca ggaaaccgtt gttcctgata taaaattatt agagtttcgc aaacaacgtt
      121 ttaatgagag agtgaatgcc atgctacatt atgcagatag cctaacatca tgtcgtagta
      181 tgatgttgct aaattatttt ggcgaaacta caaatacacg ttgtggcacc tgcgactatt
      241 gcagaaaaag aaataaaatt gaactcaacg acctgacctt tgagaaacaa aatgcagaaa
      301 taaaagattt tattcaacaa caaactttaa gatacgatga actgcttgaa aaattttctg
      361 caagtaaaaa agaacagtat gttcaatccc tgcaatggct gattgactca ggaaaaataa
      421 ttatttcgga aagcgatata cttttgtggc atgagtaagt tgcttcaatc tttttattta
      481 caggaaagtg ctgttaaagt tgcaagacag ttattaggca aagaactttg taccaagata
      541 aacggaaaac taacttccgg cattattaca gagacagaag cctatatggg tgttgaagac
      601 aaagcttcgc atgcatggaa caacagacgc acacaacgaa cagaaatcat gtatcggcag
      661 ggtggcgttg cctatgttta cctttgctat ggtattcatt cactttttaa tgttgttaca
      721 aacattcaag atgttccaca tgcagtactt ataagaagca tctttccaaa aagaggtttt
      781 gaactcatta aaaaaagaag acctgtcaac aaaccattaa aacaaattgc atcagggccg
      841 ggaatggttt cacaagcatt gggaattgat tgcagcatga caggtacatc tctacttgga
      901 aataaaatat ggattgaaaa caatgacctg caactcacag aaaaacaaat taccacaact
      961 gtccgcattg gcgttgacta tgcaggcaat gatgccatgc tgccctatcg ttttttaata
     1021 aatcctgaaa tactaaattg atgaaaaaca tatttatcag aatcagaaaa atattgtgga
     1081 agatcattaa atggttttta attttaagta ttgcaggagt aattctgttt cgttttgttc
     1141 ccataccttt tactccattg atgattatcc gatttgtgga acaggttttt gattctgaac
     1201 gtgacataca gttcaagaaa gactgggagc ctatatcaaa catttcacca ttgttggtaa
     1261 aagcagtaat caccagcgaa gaccagcgct ttaacgaaca ctttggctta gacatggagg
     1321 caattcaaaa agctgcgcaa tacaatgaaa aacataaaaa caaaaaacgt ggcgccagca
     1381 ccatttcaca acaaaccgca aaaaatgttt ttctttttcc atcgcgcaca tggcttcgta
     1441 aaggttttga agtttacttt acttttctca ttgaaatatt ctggagtaag gaacgcatca
     1501 tggaagttta tttgaatgta attgaattgg gcaatggtgt ttatggtgca gaggctgcat
     1561 cggaatatta ttttaataaa ccggcaagtc agttaagtgt acatcaggca gcatcgctgg
     1621 cagccattct tccactacct ttaaaatggt cgcctgtaaa accgaatgcg cgtgtggtgc
     1681 gaaggactga atggattaaa cgatatatga aatatgcaga ggggttggaa agcgtgaatg
     1741 gtgagaagtg atgaggtgat aaagttgaat tagatgatca gcctcggctg acaggtttga
     1801 agaatttggg aattgtctat tttgagattt gttgattaca agtattgaat gtagaggata
     1861 tttaaaggtt aagagtttat ggaattagta aacagaaact acattagatg aaaataagat
     1921 ggttatgtta agtgacactt aaatactttt tataaagtaa actgaaagaa aaacaaaaat
     1981 aatcaggtca gtccacaagg caaatttttc atttattcct gaagttttat taaatccatg
     2041 ttgtatggca gcaaatccaa agtaaagtcc gcttgcaaat gcgcttacag ttcgccactc
     2101 cggtttaaag attgaaacaa cagcaacaag tccgaaacat aagttggcaa agcctaactc
     2161 acgaagtatt ggaaaactat cggaactatc aaggtgaaaa atttcttttg ctgtaaattc
     2221 aggatttatg acttgtttag ttcctgctaa aaacaaacga agaccgacag cagaaaagat
     2281 aaaccactta ccgaacagat taaacgaaag cacagtgttg gtgaagaaat gttccgttac
     2341 aaaacatatt gcaggcaaaa taaatgtcag cacgctgaca cttattatgt aaaaccaatt
     2401 cggtttgttt tctatgtgat taatgatagt cattgaaact ataaaggtaa tattttattt
     2461 ttaaactata cagataaaaa tggagaaggt tttaaaatca gttttcggac aatgcactca
     2521 gaaaacacac aatcagggac tgctttagct taatttttta cgtagctcaa acaaaatata
     2581 tactcccatt tcagagtgcc cctgctctat ttcaacaaac cctttttgtc tgtaaaaaga
     2641 aactgctttt tggttcttta tttcgcattt caacctgaca ggcagcccaa atttatcaag
     2701 caccgcattt aacaatttag ttccaacttt ttgatcttga aaacttttgt caacatacaa
     2761 atgatgaata aaattatcag ctacccaaac agatataaat ccaattactt tttcatcaat
     2821 catagctact agaacatact caccttcggt ttctttttca aaatcagaca atttaaattg
     2881 agaagtatca acccaagaaa atgtagagcg tcttgtttct aaaaaaatat cttgcaattt
     2941 attgatatct tcctttctgc tttcaataat tttaataatg aacatattta aaaaataact
     3001 gccagagatg ttggtcatca taagaagccg gagccgatat gactatgagt tatgattgta
     3061 tttactacat tgtcatttta gtttcatcac ttgttctaat agtggtggga gtccacttgg
     3121 attaatttgg tcattgttcc aaaaactcat tacgcaacta acaaaattat ttctcaccgg
     3181 tgtggcaaca aaatatactg tcaatgtttt acctccactc atgccaacta aaactgaagt
     3241 tgcaccttta aaatcataga taatctttct tgcgttggtt tcatttcctt caaaaattac
     3301 tgcaacagtt gtatctgaaa caatcttgcc cttatttcct gctttgataa aagctatctg
     3361 attatttaca cttagtgaag catcttctaa tgttttatga acactccaat tcatttgtcc
     3421 ccaggaagga cactgcacat tgtttattcc catccatctg catccacttc ctaatggtat
     3481 ttttctgcct gcaaaattta tatcgccaat gtttaaagtc gcataaacac tttccggtat
     3541 tgcattatca tatactaatt gaacaaactc tttttccaac tccctgtctg ttttattcat
     3601 tgcaataaat gaaataccaa tgaccttgtc atcagagttt ttgaaaaagt aataattcat
     3661 atttattgta attcctgatt ctgtctttga tttagaaaca taaaagtttg aaaatcctaa
     3721 agtgctgtca gtagaaataa tttccttttc ctttatagcc aaattcttga atttcttaga
     3781 aatactttta ggtgtcaagt cggctggtac tttttgagtt gtgatttcaa tgccatcaac
     3841 attaaaaaaa tcaatgccat tgtttgaaat tccgtttagc cgagcaaaca aactgtcatg
     3901 gtcttgtccg aaagtgaaac atgaagatag aaaaaatatt actataaata tttgtatttt
     3961 ttgcatcatt ttgtcattgt gaaagtataa acaggtcttc ctttttcatc agtagtttta
     4021 atccaattgt ctttattttt aggatcatta aatgatagac tgtctgtttg aaagataata
     4081 aactttttaa accaagctgt gggagaagca tcctttttaa agtttgttct tcttgcaacg
     4141 acactgtcat tcggaaatac cataaatggt aaagtcattt cataaggtcc catttgactt
     4201 tgttttatga tgctggcttt aaagttaagt gtcttatctg tagtgttttt tacgtaaaaa
     4261 gtagtcaatg gataatttgt catacagctt acagagaaca aaactaaaat aattataacc
     4321 aagttacgca ttcgtttaaa gtgaattaga aatccaaacg tttgatataa gcactgctgc
     4381 caattactta taactattgt ttacatcaaa agtaaaaaga aatgtgatta gaatattgag
     4441 agttgaaagt tgaaaatgta aaatgcaaaa gtgaaaagtg atttgataat tatgaatgta
     4501 gaattcagag tttagaattg ttgatcagtc acggtcagcc acggctgaaa agcgtgaaaa
     4561 atgacagtct gaaaatgctt taacaagccc tgatgtttac ctgtatggtt tttgtgcgtg
     4621 agcggtgccg tgcaccgggg aaacagcagg aatagatttt gcagatgtta ctgcagtttg
     4681 agctccaaaa aattatttaa ataattacga ttttaaaaat atcgttcaga aaaaaagtac
     4741 ccggagtggt ccgccacatc caccgcaggc ggaggagaac cc
//
LOCUS       DAORQT010000112         4742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3132_length_4742_cov_5.405803, whole genome shotgun sequence.
ACCESSION   DAORQT010000112 DAORQT010000000
VERSION     DAORQT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3132_length_4742_cov_5.405803"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK134_05090"
     CDS             <1..420
                     /locus_tag="PK134_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38257.1"
                     /translation="TLYSGLKFYTQGVSGFVDVKDVSRSLISLTESKISGERFIISSE
                     NKTYEWLFKTMAKGLNINPPTIKAGMFLSQLAWRLEWLKSVFTGKPSLITNETAYTSQ
                     QIYNYDNSKITEQLNYRFIPIEETVERVCKLFIAQKQ"
     gene            complement(401..889)
                     /locus_tag="PK134_05095"
     CDS             complement(401..889)
                     /locus_tag="PK134_05095"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRB38258.1"
                     /translation="MSGNLFFRYANYNKWANQRITNAIVSAGEVAADIEMKSSFNTIR
                     KTVYHIYDSQHTWIMRIQNKPYSWPPSKDFKGDLNEFSKLLIESSQEWINYISNLNDQ
                     DFKKSISYKNTKGIDHQTPLEEIIMHCFNHSTYHRGQLVTMLRQAGVNSFEPMDFIAF
                     VR"
     gene            966..1484
                     /locus_tag="PK134_05100"
     CDS             966..1484
                     /locus_tag="PK134_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRB38259.1"
                     /translation="MIIKPPSSQQLSEYYRGYLKYLPEEDMLQTLRIATDEMESMLEK
                     IPANKETFRYAPGKWMLKEVIGHLCDTERILTYRALCFARNDKTSFPGFDENEYVANS
                     NFKNRTMQSIGIEYLIVREASLALFNSLDETDFDRIGMANQTNTSARHMLYFCIVHQR
                     HHFNIIRERYYK"
     gene            complement(1492..2613)
                     /locus_tag="PK134_05105"
     CDS             complement(1492..2613)
                     /locus_tag="PK134_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB38260.1"
                     /translation="MLLASAILIGLLSLWYTNNLVKKLSEEERKKVQLWAEASKQLAN
                     ISVEAGDISFSLMVLQDNNTVPAILTDESGNILSHINLDTAKVNDSQYVQEMLLQMKK
                     RHEPIEISFANNQKNYIYYEDSGLLYQLRYYPYFQLAIIALFLLVSYLAFSTSRKAEQ
                     NQVWVGMAKETAHQLGTPLSSLMAWIEYFKSTSQDQNHVAELEKDVARLNTITERFSK
                     IGSAPVLRKEDVYMVIENAISYIRSRTSSKVIFSLNTAQPHQVLAPLNIPLFEWVLEN
                     LFKNAVDAMDGEGKIDVMITDQLQFVYIDITDSGKGIPKSKFKTVFKPGYTSKTRGWG
                     LGLSLSKRIVEEYHNGHIFVKSSEPNKGTTFRIVLRKQV"
     gene            2831..3358
                     /locus_tag="PK134_05110"
     CDS             2831..3358
                     /locus_tag="PK134_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB38261.1"
                     /translation="MLNEQIIQGCKDKQGKAQHQLYELVAGRMMGVCLRYAKDDEEAK
                     DLLQEGFIKVFQQIDAFRGEGSFEGWIKKIFINTALESIRKRKLLTQPLEDEEDASDN
                     FLIQENKLEAKELLQMIRKLPDGYRVVFNLYAIEGFNHREIAQQLNITEGTSKSQYAR
                     ARQFLQKMLTEEKVK"
     gene            3379..4296
                     /locus_tag="PK134_05115"
     CDS             3379..4296
                     /locus_tag="PK134_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB38262.1"
                     /translation="MKEDKNIEQIFKDAFSNFEAPVDASLWQGIENSLPANPAPLTKV
                     SFLAKQAAYLKITSAVIAVATAVTAVLYFSNKNEKDTAQNVAQINKETTKAVEPATQQ
                     GVVLNNTETASKSEKINSSEEPKTKKSATVEPVKTELSDKVVENKSTQINSIPATETE
                     AILPPPQTDKQIISGPKTNTTAEPIKDTEVKKTEMKKPEAKTSVSAESKILPLPGLGN
                     MVFTPNGDGIGDWFEFDDSNLSDLSVKIYSLKGYKLVSEWHTHGGRWDGNLQNGNPAE
                     EGIYLVMIQAKGMDQTNYKSTIKLQLRRN"
     gene            4333..>4742
                     /locus_tag="PK134_05120"
     CDS             4333..>4742
                     /locus_tag="PK134_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38263.1"
                     /translation="MSKILARLTALMLVATMLVPVSSKASHLAAGDIYYTYTGTPNTF
                     LITLRLYRDCAGITMSTSETVCYTSASCNISQSITVNLVPGSGQQIPPSPCVPSAGPT
                     TCQGGTAYGIEEYLYQAVLVMPAQCIDWKFQYET"
BASE COUNT         1600 a          846 c          906 g         1390 t
ORIGIN      
        1 accttgtaca gtggattgaa gttttatact caaggtgttt caggttttgt tgatgtgaaa
       61 gacgttagcc gcagtctgat aagtttgaca gaaagcaaaa tatcaggtga gcgttttata
      121 atatcatcag aaaataaaac ttacgagtgg ttgtttaaga caatggcaaa ggggttaaat
      181 ataaatccac caaccataaa agcaggcatg tttctttcac aattagcctg gcggttagaa
      241 tggcttaaat ctgtttttac aggaaaaccg tctttaatca ccaatgaaac ggcatataca
      301 tcacagcaga tttacaatta cgataacagt aaaataactg aacaactcaa ttatcgtttt
      361 attcccattg aggaaacagt agaacgggtt tgtaaattat ttatcgcaca aaagcaataa
      421 aatccatagg ctcaaaggag tttactccgg cctgacgaag catggtgacc aactgaccac
      481 gatgataggt actgtgatta aaacaatgca tgataatttc ttccaacggt gtttgatggt
      541 ctataccctt agtattttta taggaaatgc tttttttgaa atcctgatcg ttcagattag
      601 aaatatagtt aatccattcc tgcgaacttt caataagcag ttttgaaaac tcatttaaat
      661 cacctttaaa atctttactc ggtggccagg aataaggttt attttgaata cgcataatcc
      721 aggtgtgttg cgaatcgtat atgtgataca cagtttttct tatggtattg aaactgcttt
      781 tcatttctat atctgcagca acttcccctg cacttactat ggcattggta attctttggt
      841 tagcccattt attataattg gcatatcgaa aaaatagatt tccactcatg atttttaaaa
      901 taaatttgaa tcaaatataa tattaacaga aatagtatta attttattcc ctaagattat
      961 cagatatgat tatcaaacca ccatcatcgc aacaactatc agaatattat cgtggctatt
     1021 taaaatatct tccggaagaa gacatgctgc aaacacttag aatagctact gatgaaatgg
     1081 aaagtatgct ggaaaaaatt cctgcaaaca aagagacctt tagatacgca ccgggtaagt
     1141 ggatgcttaa agaagtgatt gggcacttgt gtgatacgga acgaatttta acctaccgtg
     1201 cattatgttt tgcaagaaac gacaaaactt catttcctgg ttttgatgaa aacgaatatg
     1261 tggcaaacag caattttaaa aacaggacta tgcaaagtat aggtattgag tacctgattg
     1321 tgcgcgaagc ttcattagca ttatttaatt cgcttgatga aactgatttt gatagaatag
     1381 gaatggcaaa tcaaacaaat acttcagcca gacacatgct gtatttctgc attgttcatc
     1441 aacgccatca ttttaatatt atccgcgaaa ggtattacaa ataagctaag attacacttg
     1501 tttacggagt acaatacgga aagtagttcc tttatttggt tcagagcttt ttacaaaaat
     1561 gtgtccgtta tgatactctt caacaattct tttactcaac gaaagcccta atccccaacc
     1621 acgggttttg gatgtgtaac caggtttaaa aacggttttg aattttgatt tgggaatacc
     1681 tttgccacta tcggtaatgt caatataaac aaactgcagt tgatcggtaa tcataacatc
     1741 aatttttcct tcgccatcca tggcatcaac agcatttttg aaaagatttt caagtaccca
     1801 ttcaaataat ggaatgttta atggagcaag cacctgatgt ggttgtgctg tgttcagact
     1861 aaaaattacc ttactcgatg tgcgtgaacg tatgtaggaa atagcgtttt ctataaccat
     1921 gtaaacatct tctttacgga gtaccggtgc agatccgatt tttgaaaaac gctcggttat
     1981 ggtattcaat cttgcaacat ctttttcaag ttcagccaca tgattctgat cctgtgacgt
     2041 tgacttaaaa tattctatcc aagccatgag gctgcttaat ggtgttccca gctgatgtgc
     2101 agtttctttt gccatgccca cccaaacctg attttgttcg gctttgcgcg aagtgctaaa
     2161 agcaagataa ctcaccaata aaaatagggc aataatggcc aattgaaaat aagggtagta
     2221 gcgtaattga tataataaac cggaatcttc gtaataaata tagtttttct ggttgttggc
     2281 aaaagagatt tcaatcggct catgtcgctt tttcatttgc aacaacattt cctgaacata
     2341 ttgagagtca ttgacttttg cagtatcgag attgatgtgc gataaaatat ttccactttc
     2401 atcagttaaa atagccggca cagtattgtt atcctgcaaa accatgagag aaaaactgat
     2461 gtcgcctgct tcgacagaga tattggcaag ttgtttggat gcttcggccc aaagttgaac
     2521 ttttttacgt tcttcttcgc ttaacttttt tactaaatta ttggtgtacc acaacgacaa
     2581 tagccctatg agtatggctg aggccaaaag caaaagcttc cattgttgtt tttgtgtgta
     2641 aatattcacc cctttaaaat aaatcgtact tacagttcag gtaaaaatct atctatattc
     2701 ggatgaatta attggattac aggtgcaata atacttcttt tctttcaaaa acacagaaat
     2761 tctttttaca aaaattaaaa ccgatgcaac ccttacccct acttttgcat ctttaaagat
     2821 aatagcagta atgctcaatg aacaaatcat acaaggttgt aaagacaaac aaggcaaagc
     2881 acaacatcag ctttacgaac ttgtggcagg acgaatgatg ggagtttgcc ttcgttatgc
     2941 caaggatgat gaagaggcca aagacttgtt gcaggaaggg ttcattaaag tgtttcagca
     3001 gattgatgcc tttaggggcg agggttcttt tgaaggttgg attaaaaaaa tctttatcaa
     3061 cacagcgtta gagagcataa ggaaaagaaa attgcttacg caaccgctgg aagatgaaga
     3121 agatgcaagt gacaattttc tgattcagga aaataaatta gaagccaagg aattgcttca
     3181 gatgatcaga aagttgcccg atggttacag agtggttttt aacctctatg ctattgaagg
     3241 gtttaatcac agagaaattg cacagcagtt gaatataaca gaaggaacct caaagtcgca
     3301 gtatgctcgg gcaaggcagt ttttgcagaa gatgcttaca gaagaaaaag taaaataatt
     3361 atccgcaaag gattgaaaat gaaagaagat aaaaacatag aacagatatt taaagatgcg
     3421 ttcagcaact ttgaagcacc tgttgatgct tctttatggc aaggaataga aaattcactg
     3481 cctgcaaatc ctgctccttt aacaaaggtg tcattcttag caaaacaggc agcatatctg
     3541 aaaattacaa gtgcagtcat tgctgttgct actgccgtta cagcggtgtt atatttttca
     3601 aataagaatg aaaaagatac tgcgcaaaac gtagctcaga ttaataaaga aaccactaaa
     3661 gcagttgaac ctgctacaca gcaaggtgtg gtgttaaaca acactgaaac agcttccaag
     3721 tcagaaaaaa tcaattcttc tgaagagcca aagactaaaa agtctgcaac agttgagcct
     3781 gttaagactg agctgtcaga taaagttgtt gaaaataaat ctactcagat aaattctatt
     3841 ccggctactg aaacagaagc tattttacca ccaccacaga cagataaaca aattatatca
     3901 ggccccaaaa ccaatacaac tgctgagcca ataaaagaca ctgaggttaa gaaaacagaa
     3961 atgaagaagc ccgaagccaa aacatcagtc agtgcagaaa gtaaaatatt acctttgccc
     4021 ggccttggca atatggtttt tacaccaaat ggcgatggta taggagattg gtttgagttt
     4081 gatgacagca atctgagtga cttgtctgtt aagatttatt ccctgaaagg atataaattg
     4141 gtgagtgagt ggcacacaca tggtggccga tgggatggca atcttcaaaa cggaaatcct
     4201 gccgaagaag gaatttattt agtgatgatt caggcaaaag gtatggatca gactaattac
     4261 aaaagcacaa taaaactgca attaagaaga aattaatatt aacaaagtat aaaaactaaa
     4321 catcaaatta tcatgagtaa aattttagcc agattaacag cattgatgct ggtagcaaca
     4381 atgcttgtgc ctgtcagcag taaggcatct caccttgctg caggggatat ttattatacc
     4441 tacacaggta ccccgaatac gtttttgata acactacgtc tgtaccgcga ttgcgcaggt
     4501 ataaccatgt caacatcaga aacagtatgt tatacttctg caagctgtaa tatcagccag
     4561 agtattacag taaacttagt tccaggttca ggacagcaaa ttccaccatc accatgtgtg
     4621 ccttcagcag gcccaacaac ctgccagggt ggtacagctt atggtattga agaatatctg
     4681 tatcaagctg ttttggtaat gccggcacaa tgtattgact ggaaattcca gtatgaaaca
     4741 tg
//
LOCUS       DAORQT010000113         4717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3157_length_4717_cov_19.386315, whole genome shotgun sequence.
ACCESSION   DAORQT010000113 DAORQT010000000
VERSION     DAORQT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3157_length_4717_cov_19.386315"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PK134_05125"
     CDS             <1..915
                     /locus_tag="PK134_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRB38264.1"
                     /translation="MMRAIRFATQLNFSIEEKSLAAIQSEKSRISIVSKERITDELNK
                     IIASPLPSTGFKLLMDTGLLELIFPQFYNLKGVDYFEGKGHKDNFYHTLEVLDNIAKV
                     TDNLWLRWAAILHDIAKPATKRFEPGHGWTFHGHEDKGSRMVPPIFRELKLPLNEKMK
                     YVQKLVLLHLRPIVLAQELVTDSAVRRLLFDAGDDIDDLMLLCQADVTTKNEYKKAKY
                     RNNFELVIQKLKEVEEKDRIRNWQPPVTGQLIMDTFGLPPGPNVGIIKDFVREAILDG
                     DCPNEQQAAFELMLKKGLELGLTPQKKS"
     gene            993..2804
                     /gene="typA"
                     /locus_tag="PK134_05130"
     CDS             993..2804
                     /gene="typA"
                     /locus_tag="PK134_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRB38265.1"
                     /translation="MQNIRNVAIIAHVDHGKTTLVDKILHQTQVFRQNQQTGELILDN
                     NDLERERGITIVAKNVSVQYKDVKINIIDTPGHADFGGEVERVLTMADGVILLVDAFE
                     GPMPQTRFVLQKALALGLKPIVVINKVDKENCRPDEVHESVFDLFFHLEATEEQLDFP
                     TLYGSSKHGWMSTDWRKETSDITALLDAIIEKTPPAKSLEGTPQMLITSLDYSNYVGR
                     IAIGRIVRGTLKENMPVTLMKRDGSAKKSRIKELFVFEGLGRKKVDSVSSGDIAAIVG
                     VDDFEIGDTIADFENPEALKSIAVDEPTMSMLFTINNSPLFGKEGKFVTSRHLRDRLY
                     KETEKNLAMRVEETNSPDAYLVYGRGILHISILVETMRREGYELQLGQPQVLYKEIDG
                     IRCEPVEHLVVDTPSEVAGKVIELATQRKGELTSMLPKGDLQRLEFNIPARGLIGLRN
                     AVLTATAGEAIMAHRFNSYEPAKGTIPGRNMGVLLAKEAGSSTAYSIDKLQERGAFFI
                     DPGETVYGGQVIGEHIRPGDLVVNVCEGKKLTNIRASGSDDNARITPKIKFSLEEAME
                     YIEKDEYLEVTPQNIRMRKIYLDENERKRMSKKAEMA"
     gene            2869..3258
                     /locus_tag="PK134_05135"
     CDS             2869..3258
                     /locus_tag="PK134_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRB38266.1"
                     /translation="MKAVQKKQKVKMEFPMRSSPKILYGFLSTPSGLSEWFADDVTIR
                     EGVYTFHWDGDEQQAKMTHKRENQMVRFKWIDDSDDAFIEFEITTDELTSDVSLMVSD
                     FAVEDDIEETQRLWDTQIHNLRHLIGS"
     gene            complement(3311..4015)
                     /locus_tag="PK134_05140"
     CDS             complement(3311..4015)
                     /locus_tag="PK134_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRB38267.1"
                     /translation="MLLISVITKNKKRSRKCLYWAIGAILFFSNPFVLNLVMHAWEIQ
                     SVSIDSIHKEYDAAIMMGGAIRYVNGQMQRPVFGSGADRYVQSLELFYRNKAKYIIIS
                     SGSGSLVYTDVKEADLLRNQMIRIGVPADKVIAENQSRNTYENAKNTAEIIRKSDFRP
                     PFLLVTSAFHMRRSMLCFQKQGIDVVPFAVDQHSGQIMFTPDKLFLPSPEVLLDWDLL
                     FHEWFGIAVYKVMGYL"
     gene            complement(4152..>4717)
                     /locus_tag="PK134_05145"
     CDS             complement(4152..>4717)
                     /locus_tag="PK134_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRB38268.1"
                     /translation="RKIEQLTGKLRDVKSELKSYKKNSPAYNSNKKKVDNVAAELSTL
                     KEVYRNVFRKDELERTINYAPLVHRLVADSFVKQPSKKHDIVAHLDYNKSNNHFSNLK
                     WMTQEDNIEHQKKSPVVIKEKKKRIGKRPENASGYKLNEKKVAEIKKRIQKGETLSKI
                     SDRFNVSETQLLRIKRGINWGDVKVPH"
BASE COUNT         1551 a          843 c          907 g         1416 t
ORIGIN      
        1 atgatgcgtg caatacgctt tgcaacacaa cttaatttca gcattgaaga aaaatcgctg
       61 gctgcaattc aaagtgaaaa gagtagaatc agcatagttt ccaaagaacg aataaccgat
      121 gaactgaata aaattattgc ttcgccattg ccatcaacag gattcaaatt gttgatggat
      181 acaggattgt tggagttaat atttccgcag ttttacaacc tgaaaggagt tgattatttt
      241 gaaggcaaag gtcataaaga taacttctat cacaccttgg aagtattgga caacattgca
      301 aaagtcactg ataatttatg gttacgatgg gctgctattc tgcatgacat tgcaaaacct
      361 gcaacaaaac gttttgaacc cgggcatgga tggacttttc atgggcatga agacaaaggt
      421 tcacgtatgg ttcctcctat cttcagagag ttaaaattac ctctcaatga aaagatgaag
      481 tatgtacaaa aattggtttt gctccattta agacccattg ttttggcaca ggaacttgtt
      541 accgattcag cagtacgcag actgttattt gatgccggtg atgatattga cgacctgatg
      601 ttgttgtgcc aggctgatgt gacaactaaa aatgaatata agaaagccaa ataccgtaac
      661 aactttgagt tggtcattca aaaacttaaa gaagttgaag agaaagacag aatcagaaac
      721 tggcaaccac ctgttaccgg acaattgatt atggatacat ttggtttgcc gcctggacct
      781 aatgttggta ttataaaaga ttttgttcgc gaagcaattt tagatggcga ttgtcctaat
      841 gaacaacagg cagcatttga gttaatgctt aaaaaaggcc tcgaacttgg gctaacacca
      901 caaaaaaaga gctgattttt tctactttta ttattgacaa tcaatatttg ggcggtaaaa
      961 agtcttatct ttgcacccca aataaaattt tcatgcaaaa tatcagaaat gtggccatta
     1021 ttgcccacgt tgaccatggt aaaacaacct tagttgataa aatacttcat cagacacagg
     1081 ttttcagaca aaatcagcaa acaggagaat tgattctcga caataatgat ctggaacgtg
     1141 agagaggaat taccattgtt gcaaaaaatg tatctgttca gtacaaagac gtaaaaatta
     1201 atattattga cacaccgggt cacgctgact ttggaggaga agtagaacgt gttcttacaa
     1261 tggctgacgg tgtaattctt ttagtagatg cttttgaagg accaatgcca cagacacgct
     1321 ttgttttgca gaaagcactt gcattaggac ttaagccaat cgttgtcatc aacaaagttg
     1381 ataaagaaaa ctgtcgccca gatgaagtac atgaatcggt atttgatttg ttttttcatc
     1441 ttgaagcaac agaagagcaa cttgactttc caacattata tggtagtagt aaacatggtt
     1501 ggatgagcac agactggcgc aaagaaacat cagacataac agctttactt gatgccatta
     1561 tagagaaaac gcctcctgca aaatcgcttg aaggcacacc tcagatgctc atcacatcgt
     1621 tagattacag caactatgtt ggccgtattg ccattggcag aattgtgaga ggaacattga
     1681 aagaaaatat gcctgtgacg ttgatgaaac gtgatggtag tgcaaaaaaa tcaagaataa
     1741 aagaactgtt tgtttttgaa ggattaggca gaaaaaaagt tgacagcgta agcagtggcg
     1801 acattgcagc tattgtaggt gtagatgatt ttgaaatcgg tgataccatt gcagattttg
     1861 aaaaccctga agcattgaaa tcaattgcag ttgatgaacc cacaatgagt atgcttttta
     1921 ccatcaacaa cagtccgctg tttggtaaag aaggaaagtt tgttacctct cgccaccttc
     1981 gtgatcgtct ttataaagaa acagaaaaga atctggccat gcgtgttgaa gaaaccaatt
     2041 caccggatgc atatcttgtt tatggacgtg gtattctgca catttctatt cttgttgaaa
     2101 ccatgcgcag agaaggctat gaacttcagt taggacagcc acaggttttg tacaaagaaa
     2161 tagatggtat tcgttgcgag ccggtagagc atttagttgt tgatacacca tcagaagtag
     2221 caggtaaagt gattgaactt gccacacagc gtaaaggaga acttacaagc atgttgccta
     2281 aaggtgattt acaaagactt gaatttaata ttcctgcaag aggattaatt ggtttaagaa
     2341 atgcagtact tactgcaact gcaggagaag caattatggc acatcgtttc aattcttatg
     2401 agcctgctaa gggtactatt cccggaagaa atatgggtgt gctgcttgct aaagaagcag
     2461 gaagttcaac agcctactct attgataaat tacaagagcg tggagcattt tttattgatc
     2521 cgggtgaaac agtttatggt ggacaagtta tcggtgaaca tatccgtccg ggcgacttgg
     2581 tggtgaatgt atgtgaagga aaaaaattaa ccaacatcag agcatcaggc agtgatgaca
     2641 atgcgcgtat tactccaaaa ataaaattct cgttagagga agcaatggag tatattgaga
     2701 aagatgagta tcttgaagtt actcctcaga atattcgcat gcgtaaaatt tatcttgatg
     2761 aaaatgagcg taagcgcatg agtaagaaag cagaaatggc atagtgaaat aagttaggat
     2821 tgtataagtt tttttattat tattgtaaaa attaaacaat aacatacaat gaaggcagtg
     2881 cagaaaaagc aaaaagtaaa aatggagttt ccaatgcggt cttctcccaa aatattatac
     2941 ggattcctat ccactccatc agggctatct gaatggtttg ccgatgatgt cactattcgt
     3001 gaaggtgttt acactttcca ttgggatggc gatgagcagc aagccaaaat gactcacaaa
     3061 agagaaaatc aaatggtgcg tttcaaatgg attgatgaca gcgatgatgc ttttatcgaa
     3121 tttgaaatta caaccgatga attgacatct gatgtatcac taatggtatc tgactttgca
     3181 gtagaagatg atattgaaga aacacaacgt ttatgggaca cacaaatcca taatttgcgt
     3241 catctgattg gatcatagtg ttacactaat atctctatac attgttttga tattagactt
     3301 aatatttatc ttacaaatac cccattactt tataaactgc tattccaaac cattcatgaa
     3361 atagcaagtc ccaatcaagt aacacctccg gtgatggcaa aaaaagttta tcaggtgtaa
     3421 acattatctg acctgaatgt tggtcaactg caaaaggcac cacatcaatt ccttgtttct
     3481 gaaaacaaag cattgacctc cgcatatgaa atgccgatgt cactaataaa aatggtggtc
     3541 taaaatctga ttttcgaata atttcagcag tatttttcgc attttcataa gtgtttcttg
     3601 actgattttc tgcaatcact ttatctgcag gaacacctat gcgtatcatc tgattacgca
     3661 gtaaatctgc ttctttaaca tcagtataaa ccaaactgcc cgaaccactg gaaataatta
     3721 tatactttgc tttgtttcta taaaaaagct ccagcgactg aacatatcta tcggcacctg
     3781 aaccaaaaac cggacgttgc atctgtccgt ttacataacg tatagcacca cccatcatta
     3841 ttgccgcatc atattcctta tgaattgaat ctatagatac cgactgaatt tcccatgcat
     3901 gcattactaa attcagcaca aacggattgc tgaaaaatag tattgctcct attgcccaat
     3961 ataaacactt ccggcttcgt ttcttattct ttgtaataac agaaataagc aacaagatta
     4021 cgatccaact tagtggcgat aataaaaaat ctaaaatctt tgaaaggaaa aaaaatatgt
     4081 gttgcattaa atcaagttac aacaaaaaaa tcagacagat gagtaaacaa aaatcagaca
     4141 gatgagtaaa cttaatgggg aacttttaca tcgccccagt ttattcctct cttaattcga
     4201 agtagttgcg tttccgaaac attaaatcgg tcagatattt tagataatgt ctctcctttt
     4261 tgaattcgtt tctttatttc agctactttc ttctcattga gtttatagcc cgatgcattt
     4321 tccggacgct taccaattcg cttcttcttc tcttttataa ctacgggact tttcttctga
     4381 tgttcgatat tatcttcctg agtcatccac ttcaggtttg aaaaatgatt attgcttttg
     4441 ttataatcaa gatgtgcaac aatatcatgt tttttagaag gttgcttaac gaaactatct
     4501 gcaacaagac gatgcaccaa tggagcatag ttaattgtgc gctctaattc atcttttcgg
     4561 aatacatttc tatacacttc ctttaaggtg ctcaactctg cagcaacatt atcaactttc
     4621 tttttattgc tgttataggc aggtgaattc tttttgtaag attttaattc tgattttaca
     4681 tcgcgcagtt tacctgttag ttgttcaatt tttcttc
//
LOCUS       DAORQT010000114         4601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3280_length_4601_cov_6.867576, whole genome shotgun sequence.
ACCESSION   DAORQT010000114 DAORQT010000000
VERSION     DAORQT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_4601_cov_6.867576"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK134_05150"
     CDS             complement(<1..205)
                     /locus_tag="PK134_05150"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRB38269.1"
                     /translation="MNIQKIALLTSGGDSPGMNACIRSIVRSAISQKKKVVGIMRGYE
                     GMIHGEFVELNARSVSNILQRGGT"
     gene            299..2098
                     /locus_tag="PK134_05155"
     CDS             299..2098
                     /locus_tag="PK134_05155"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRB38270.1"
                     /translation="MTKFTLLAAFAVLFSGASIAQDRVPVNQNPAIGARSQSVIATEF
                     TNSGQLIPRNGNSAINVAFSDDFSQQNDTSSLIARGYLPYYRGTGAQGISATWFQPDG
                     TVFPAYNGAISEYVAANYNVVTATNNIDSWLVTPALNVASGDKISFYARSVTSSTWVD
                     SIRVMYSAAGDSTPEGLTWVELGRFQASVAGTWDYINYAVTTAGATARFAIRYAVIDG
                     GPNGSNSNFIGVDQLDIYTPQTTDASVFAISSMNSGCGLSATTPVDVSIFNWGGSAIS
                     GFPVSFVVDNGTPVTETFTGTIPAGGSSSFLFTGTADFSAVGPHTIKAYSSLSGDGNA
                     ANDTTTATVTNIPPIVLGSTAVTEGFEAASGTTPPPGWTIEDTDGDGNIWDFATTYTH
                     NGLVCARLGVAQAMAGTPENWLFSPCLDLTAGTNYKIEYWYKSFTETATSYQLELRYG
                     NAATGAAMTNNIAVDPLFTDSTYHLASHTFTPANSGVYNIGINGFGSGVDNSIRVDDA
                     SMVVVTGIKETDLSSNVTLMPNPATTELLVSAKLYGNSTLTVYNQLGQEVIKQNYNAP
                     FRITLDIQSLKKGVYTLKISNADGVVVNKFVKQ"
     gene            2161..2520
                     /locus_tag="PK134_05160"
     CDS             2161..2520
                     /locus_tag="PK134_05160"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRB38271.1"
                     /translation="MKTSDSFNADEISFFEKVYQVVRLIPKGKVTSYGAIAKYLGSAQ
                     SSRMVGWAMNNSSRIKPKVPAHRVVNRNGMLTGKHFFGANDAMQHLLEKEGIKVSDDK
                     IIDFKKHFWDPSVELML"
     gene            2551..3651
                     /locus_tag="PK134_05165"
     CDS             2551..3651
                     /locus_tag="PK134_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRB38272.1"
                     /translation="MQITKEKVLEALSYVQEPDLKKDLVTLNMIKDIEIEGQNVSFTV
                     ILTTPACPLKAEIERACKNAIVHFVDKDAVVTVNMTANVTTQRSMNNEMMKGVKNIIA
                     VASGKGGVGKSTVAANLAVSLAKTGAKVALVDADIFGPSVPIMFGSEHEHPFIEEVNG
                     RQLMIPVEKHGVKLISIGYLADPSQAIVWRGPMASKALKQLFSDVNWGEIDYMIVDLP
                     PGTSDIHLTLISAVPLTGAVVVTTPQDVALADARKAIAMFQLPSVNIPVFGLIENMSW
                     FTPEELPDNKYYLFGKDGGQKLAAEMNVKLLGQVPLVQSVREGGDIGNPIATGNSIVS
                     EAFNELSKSVAQQVAIRNAVIEPAQRVPAAGF"
     gene            3663..3917
                     /locus_tag="PK134_05170"
     CDS             3663..3917
                     /locus_tag="PK134_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRB38273.1"
                     /translation="MSESLKNNLKHKVEQALDTIRPYLKSDNGDVLDITPQNVVKLRF
                     EGACSSCTMSAMTFKAGVEETILRMVPEITAVEALNVENV"
     assembly_gap    3971..3987
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3988..>4601
                     /locus_tag="PK134_05175"
     CDS             <3988..>4601
                     /locus_tag="PK134_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38274.1"
                     /translation="PMKITAAVVVSILFFTGTIHAQTFVVFKGDTINRKDAKGLKQGI
                     WKKFYRTDSLFSITSFKDNKQTGTTKFYYESGKLKSTLIWKKPGQLAFAESFFEEGKK
                     MSDGYYLNEQKDSTWHYYNEEGKITSQETYRKGVKQGKWLTYYPEGGKVEEKNYMSGK
                     LEGPYLKFFKDGKLMLKATYKNDKLTGGYTIYNADGGFYTTGQY"
BASE COUNT         1487 a          785 c          919 g         1393 t
ORIGIN      
        1 tagttccacc tctttgtaaa atattgctga cagagcgggc atttaattct acaaactcac
       61 catgaatcat tccttcataa ccccgcatga tgccgactac ttttttcttt tgagaaatgg
      121 cgctgcgcac tattgaacga atacaggcat tcattcccgg agaatcgcca ccggaagtaa
      181 gtaaggcaat tttttgaatg ttcataattt ttgaaaaaag aataggcaaa tttaatccat
      241 tagaatctct ttttttactt ttacaccatt aaaccattat aaatttaaaa aatcacaaat
      301 gacaaaattt acactattag ctgctttcgc agtgttgttt tccggtgctt ccatagcgca
      361 agaccgggtt cctgtgaatc aaaaccctgc tattggggca agatcacagt ctgtaatagc
      421 tacagagttc acaaattcag gacaattgat tccaagaaat ggaaacagtg ccatcaatgt
      481 tgctttttct gatgacttca gtcaacagaa tgacacttct tctttgattg ccagaggtta
      541 ccttccttat tacagaggta caggtgcgca aggtatatct gcaacatggt ttcaacctga
      601 tggaacagtt tttcctgcat ataatggcgc tatctctgaa tatgtagctg ccaattataa
      661 tgttgtaact gcaacaaata atattgatag ctggttggtt acgcctgcat taaatgttgc
      721 atcaggcgat aagatttcat tttatgcaag aagtgttaca agttctacat gggttgattc
      781 aattcgtgta atgtactctg cagccggtga ttctactcct gaaggtttaa cttgggttga
      841 attaggaaga tttcaagcaa gtgttgcagg aacgtgggac tatattaatt atgccgttac
      901 tactgcaggc gctactgcac gttttgcaat tcgttatgct gttatagatg gtggtcctaa
      961 tggttcaaac agtaatttta ttggagttga ccaattagat atttatacac cacaaacaac
     1021 tgatgcaagt gtgtttgcaa taagcagcat gaattcaggc tgtggtttaa gtgcaacaac
     1081 accagtcgat gtttcaatat tcaattgggg tggaagcgca atttccggat tcccagtatc
     1141 atttgttgtt gataatggta cgcctgtaac tgaaactttt actggaacaa ttccggcagg
     1201 aggttcatca tcatttcttt tcacaggtac agctgatttt tctgcagttg gaccacatac
     1261 aataaaagct tatagcagtc tttcaggaga tggtaatgcg gcaaatgata caactactgc
     1321 aactgtaaca aatatacctc caatagtact aggttctact gcagtaacag aaggttttga
     1381 agctgcttca ggaacaacac cgcctccggg atggacaatt gaagacaccg atggtgatgg
     1441 taatatatgg gactttgcta ctacctatac ccataatggt ttagtatgtg ctcgtcttgg
     1501 tgttgctcag gcaatggcag gcactcctga aaactggtta ttctcaccat gtcttgattt
     1561 aactgcagga acaaattata aaattgaata ttggtataag agttttacag aaactgcaac
     1621 ttcatatcaa ttagaactgc gttatggaaa tgcagccaca ggtgctgcca tgaccaataa
     1681 tattgctgtt gatccattgt ttactgattc aacttatcat ttggcttctc acacattcac
     1741 cccagcaaac tcaggtgttt ataacatcgg tataaatggt tttggttctg gtgtagacaa
     1801 ttcaatcaga gttgacgatg cttctatggt agtagtaaca ggtataaaag aaactgattt
     1861 aagcagcaat gtgacactta tgccaaatcc tgctacaaca gaattattgg tttctgctaa
     1921 actatatggc aactcaacac ttactgttta taatcaatta ggtcaggaag ttattaagca
     1981 aaactataat gcgcctttcc gcatcacatt agatattcaa tctttgaaga agggtgttta
     2041 cacacttaaa ataagcaatg ccgatggtgt tgtagtaaat aagtttgtaa aacaataatt
     2101 aatcttttta tacaaaaacc accggctttc agtcggtggt ttttgtattt ttatacctgt
     2161 atgaaaacat cagattcctt taatgccgat gagatttctt tttttgaaaa agtatatcag
     2221 gtagttcgtt taattccgaa aggaaaagtg actagttatg gtgccatagc caagtatctt
     2281 ggcagcgcac agtcatcacg tatggttggt tgggcgatga ataatagtag tcggataaaa
     2341 ccaaaggttc ctgcacacag agtcgtaaat cgaaatggca tgctcacagg aaaacacttt
     2401 tttggtgcta atgatgccat gcaacatttg ctcgaaaaag aaggcataaa agtatcagac
     2461 gataaaataa ttgattttaa aaaacacttt tgggatccat ctgttgaatt gatgctataa
     2521 acactcattt acaaccatta tctttgcacc atgcagatta caaaagaaaa agtattagaa
     2581 gctttaagtt atgtacagga acctgatctt aaaaaagatt tggtgacact gaacatgatt
     2641 aaagatattg aaattgaagg gcagaatgtt agttttaccg taatccttac aactccagct
     2701 tgtcctctga aagctgaaat tgagcgcgcc tgtaaaaatg ccatagttca ttttgtagat
     2761 aaagatgctg ttgtaacagt aaatatgact gccaatgtta ctacacagcg cagcatgaat
     2821 aatgaaatga tgaaaggcgt taaaaatatt attgctgttg cctccggcaa aggtggtgta
     2881 ggtaaatcta ctgttgctgc taatttagca gtttcactag ctaaaactgg tgctaaagtt
     2941 gcccttgttg atgccgatat atttgggcct agtgtgccaa ttatgttcgg ctcggagcat
     3001 gagcatcctt ttattgaaga agtaaacgga agacaattaa tgataccggt agaaaagcat
     3061 ggtgtaaaac ttatttcaat tggttacctt gcagatccat cgcaggcaat tgtttggcga
     3121 ggacctatgg catcaaaagc attgaaacaa ctttttagtg acgttaactg gggcgaaata
     3181 gattatatga ttgttgacct tcctccggga acaagcgata ttcatcttac attaatctcc
     3241 gcagtacctc tgacaggtgc agtagtggta acaacgccac aagatgtagc attagccgat
     3301 gccagaaaag caatagcaat gtttcagctt ccatcagtaa atattcctgt ttttggatta
     3361 atagaaaata tgtcgtggtt cactcctgaa gagcttcctg acaataaata ttatcttttt
     3421 ggaaaggatg gtggacaaaa attagctgca gaaatgaatg taaaattact cggtcaggtt
     3481 ccattagttc agtctgttcg cgaaggtggc gatataggaa atccaattgc aacaggaaat
     3541 tcaatagttt cagaagcatt taacgaatta tcaaaatctg ttgcacaaca ggtagcaatt
     3601 cgtaatgctg ttattgagcc ggcacagcgt gttcctgctg caggatttta aaaagtgtaa
     3661 gaatgagtga gtcattaaaa aataatttaa aacataaggt cgagcaggca ttggatacca
     3721 ttcggcccta tttgaaatcc gataatggtg atgttcttga tattacacca caaaatgttg
     3781 ttaagttgag gtttgaagga gcatgcagtt cgtgtacaat gagtgcaatg acatttaaag
     3841 caggagttga agaaactatt ttacggatgg tgcctgaaat tactgctgta gaagcattga
     3901 atgtggaaaa tgtttaaatg gtaattttat ctaaaataaa acagacttgt tgatgttatc
     3961 tcttaataag nnnnnnnnnn nnnnnnnttc caatgaaaat tacagctgct gttgttgttt
     4021 ctattttatt ttttaccggc actatacatg cacaaacttt tgttgttttc aaaggcgata
     4081 ctataaatcg taaagacgct aaaggactta aacaaggtat atggaaaaag ttttacagaa
     4141 cagacagctt gttttcgata acatcattta aagacaacaa acaaactggt acaacaaaat
     4201 tttattacga atcaggtaaa ttaaagtcaa cactcatttg gaaaaaaccc ggtcagcttg
     4261 cttttgcaga aagctttttc gaagaaggaa aaaaaatgtc tgatggttat tatctgaatg
     4321 aacagaagga cagcacttgg cattattata acgaagaggg taaaattaca tcacaggaaa
     4381 cctatcgcaa aggtgtaaaa caagggaaat ggcttaccta ttatcctgaa ggtggaaaag
     4441 tagaggagaa aaattatatg agtggtaaat tggaagggcc atacttaaaa ttttttaaag
     4501 atggaaaatt aatgctaaag gcaacctata aaaacgataa acttacaggt ggttatacca
     4561 tctataatgc agatggtgga ttttatacaa cagggcaata t
//
LOCUS       DAORQT010000115         4598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3285_length_4598_cov_5.134933, whole genome shotgun sequence.
ACCESSION   DAORQT010000115 DAORQT010000000
VERSION     DAORQT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_4598_cov_5.134933"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>948)
                     /locus_tag="PK134_05180"
     CDS             complement(<1..>948)
                     /locus_tag="PK134_05180"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRB38275.1"
                     /translation="NAGDSVVLNYSWYITPAALSSPVYYQLSVVAFVQDNSSKEVLQA
                     GFSRAQVPMDPKAKAITGMQDVTCSGTMTPSFTVANNGITDISTLDIQYKVDNNPAQT
                     YSWMGLLPSFTSTTISLPPIPVSGNGSHSITLTTANPNGGVTDNNMLNNSKTAYFGIP
                     GAAVAGNVLEDFTLSTFPPANWIRVDQNGNNTGWLRSTAGNGSAKIDFYSSFTGHIDN
                     LYLYPVDMSGASGATMTFDVSHRQYSAAYIDAINVQVSTDCGLTWNTEWAKSGAALAT
                     VTGYQTGAFTPSAAQWRAETVNLNQYAGNNNVLIRFNAV"
     assembly_gap    949..958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<959..1553)
                     /locus_tag="PK134_05185"
     CDS             complement(<959..1553)
                     /locus_tag="PK134_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38276.1"
                     /translation="MRKISTLFAMLCITTAVSAQTQRLVLFEEFTGETCGPCALVNPT
                     LNEMLNDEETKIVSIKYQNNIPSPGPRFWPYAQSDIAARQAYYNNTYSPHGIMDGNQF
                     NDNAGALDPVIINPRYATPSPFEVRVAHTFSAANDTIYASVIIKAAQSYSDSNLVAQI
                     AVTERDIFGYTSPNGETHYEGVLRKMLPSAYGTPLQIS"
     gene            complement(1617..3071)
                     /locus_tag="PK134_05190"
     CDS             complement(1617..3071)
                     /locus_tag="PK134_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRB38277.1"
                     /translation="MHNDKPHLHRSLGLWDATMLVAGSMIGSGIFIVSADMSRHTGSA
                     GWLLFLWLLSGVITLIAALCYGELAGMMPQAGGQFVYIRRAWGNCLAFLYGWTVFMVI
                     QTGVIAAVAVAFAKFTAVFFPYFNTGNILFQAGGISISAAQMLAIFLIAVLTWFNSRG
                     IEGGKTIQNIFTSAKLLALFILIVAGLWVGLKTNTLSTNFGNAWSVNSMIKTDSGWIE
                     QHISGFAIILMLGTAIIGSLFSSDAWNNVTFIAGEIKDPSRNIPRSLMLGTAMVTLLY
                     CLANIAYLALLPLKGSIDAATVAGQGIQFAGGDTDRVGTAAASMIFGDSSVYIMAALI
                     MISTFGCNNGIILSGARVYYAMALENLFFKQAKKLNSKGVPAIALWVQAIWAGLLCLS
                     GTYGDLLDYTVFASLLFYVITISGIFILRKREPDAERPYKVFGYPFLPALYIILALSI
                     CSILLYTKTAVAGKGLLIVLLGLPIYYLFKRRSS"
     gene            complement(3093..4565)
                     /locus_tag="PK134_05195"
     CDS             complement(3093..4565)
                     /locus_tag="PK134_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRB38278.1"
                     /translation="MSKGIHVKKPLDSLLAEAEDSEKGLKRTLTANSLIALGIGAIIG
                     AGLFVRTAAAAANNAGPAVTLAFVIAGLGCAFAGLCYAEFASMIPIAGSAYTYSYTTM
                     GEFIAWIIGWDLVLEYALGAATVSIAWSEYLNKLLNYLGMHIPYEWCHSPFEHSLDGV
                     YGIMNVPALVILILLSLLLIKGTQESAFVNGIIVVTKVAIVILFIVFGWQFINPANHT
                     PYIPAASTFTDHMGVPHHYGGILGILGAAGVVFFAFIGFDAVSTAAQEAKNPGRDMPK
                     GILGSLIICTVLYILFAHVLTGVASVEDFRTQGREASVTYAIQTYMHGYDWLAKLVTV
                     AILAGFSSVILVMLLGQSRVFYSMSRDGLLPKVFSELHPVYRTPYKSNLILFVFVGLF
                     AAFVPGDIVGDMTSIGTLFAFMLVAAGVIIMRKTNPDAKRTFRTPLVPLVPILGIIVC
                     GAMIFGLGWPNWSRLLMWLAIGFVIYFGYSAKHSKLRNPK"
BASE COUNT         1465 a          943 c          899 g         1281 t
ORIGIN      
        1 ctcactgcat taaaacgaat aagtacattg ttgttgcctg catattgatt caggtttacg
       61 gtttcagcac gccactgtgc tgctgaaggt gtaaaggcgc ctgtctgata gcctgtaact
      121 gttgcaagag cggcacccga tttagcccat tctgtattcc atgttagtcc gcaatctgta
      181 gaaacctgaa catttattgc atcaatatag gcagcactat attgacgatg tgaaacatca
      241 aaagtcatag tagcacccga tgcaccactc atgtcaaccg gatatagata aagattatca
      301 atgtgaccgg taaagctgct ataaaaatca attttagcag atccatttcc tgcagttgaa
      361 cgtaaccatc cggtattgtt tccattctga tcaacacgaa tccagttagc tggcgggaaa
      421 gttgataaag taaaatcctc caaaacattt cctgcaacag cagcacccgg aataccgaaa
      481 taagcagttt tgctattgtt aagcatatta ttatccgtaa caccgccatt aggattcgct
      541 gtagttaaag taatactatg acttccattt cctgaaaccg gaattggtgg caaggaaatg
      601 gttgtacttg taaaacttgg caacagtccc atccaggaat atgtctgcgc aggattatta
      661 tctactttat attgtatgtc taaagttgaa atgtcagtta tgccattgtt tgcaactgta
      721 aatgatggtg tcattgtacc gctgcaagta acatcctgca tgcctgttat tgcttttgct
      781 tttgggtcca taggaacttg tgcacgactg aaacctgcct gaagtacttc tttgctagag
      841 ttgtcctgaa caaatgcaac tactgatagc tgataataaa caggagatga aagtgcagca
      901 ggtgtgatgt accacgaata atttagaaca acagaatcgc ctgcattcnn nnnnnnnnat
      961 gagatttgca atggcgttcc gtatgcagaa ggcaacattt tacgaagtac tccttcgtag
     1021 tgtgtttcac cattaggtga tgtgtatcca aaaatatctc gttcggtaac tgcaatctgt
     1081 gcaaccaagt ttgagtcgct ataactttgt gctgctttta ttattacaga agcataaata
     1141 gtgtcgttgg ctgcactgaa agtatgagca accctaactt caaatggtga aggtgttgca
     1201 taacgtgggt tgatgataac aggatctaaa gcacctgcat tatcgttaaa ctgattgcca
     1261 tccataattc catgaggtga ataagtgttg ttgtaatatg cctgacgggc tgcaatatct
     1321 gattgtgcat aaggccagaa tcgcggaccg ggagaaggaa tgttgttttg atacttaata
     1381 gaaacaattt ttgtttcttc atcattaagc atttcgttga gtgtaggatt taccagtgca
     1441 catggcccgc aggtttcacc ggtaaattcc tcgaaaagaa caaggcgttg tgtttgtgct
     1501 gaaacagcag ttgtaataca caacatagca aatagtgtag agatttttct catttgaatg
     1561 acatttatat tttgaactac gaatgtacgt agcttttaaa tacctgagaa atctatttaa
     1621 ctgctgcgtc ttttaaacag ataatagatg ggtaatccta gcagaacaat aagtaagcct
     1681 ttgccggcaa cggctgtttt ggtataaagt aatatagagc aaattgatag tgctaaaata
     1741 atatagagtg ccggtaaaaa tggataacca aatactttgt aaggcctttc tgcatcaggc
     1801 tcacgtttac gcaaaataaa aataccggaa atagttatta cataaaacag gagtgaggca
     1861 aaaacagtgt aatcaagtaa atcaccatac gtgccgctta gacataataa acctgcccat
     1921 attgcctgta cccatagcgc aattgctgga actccttttg aattgagttt ttttgcctgc
     1981 ttaaaaaaga gattttccaa agccattgca taataaactc tggcaccact aaggataata
     2041 ccattgttgc atccgaaagt tgaaatcatg atgagtgctg ccatgatata aacacttgag
     2101 tcaccaaaaa tcatagaggc tgcggctgtt cctacccggt cggtgtcgcc tcctgcaaac
     2161 tgaatacctt gccctgcaac tgttgcagca tcaatactgc cttttaaagg taataatgcc
     2221 agatatgcaa tgttggctaa gcagtaaagt aaagttacca ttgctgtgcc caacatcaga
     2281 cttcgtggaa tgttgcggct cggatctttt atttcaccgg caataaatgt aacgttgttc
     2341 caagcatcgc tgctgaataa tgagcctata atggcagtgc caagcatgag tattattgca
     2401 aagccactga tgtgttgttc tatccagccc gaatcggttt taatcattga attaacgctc
     2461 caggcattgc cgaagttagt tgaaagcgtg ttggttttta atccaaccca tagtccggct
     2521 acaatgagaa taaatagggc aagcaacttt gctgaagtaa aaatgttttg aatggttttt
     2581 ccaccttcaa taccacggct gttgaaccat gttagtacag caatgagaaa tatagcaagc
     2641 atttgtgcag cagatatact tattccgcct gcctgaaata aaatattgcc tgtgttaaaa
     2701 taaggaaaaa acacagcagt aaattttgca aatgctacag ccactgcagc aataactccc
     2761 gtttgaatga ccataaatac tgtccatccg tataaaaatg ccaagcagtt tccccatgca
     2821 cgtctgatat aaacaaactg acctcctgct tgcggcatca tacctgccag ttcgccataa
     2881 caaagtgctg ctataagtgt gataacacca cttagcaacc ataaaaaaag caaccatcct
     2941 gcagaacctg tatgccgtga catgtcggca ctgacaataa aaatacctga gccaatcatt
     3001 gaaccggcaa ccaacatggt agcatcccac agtcctaatg aacggtgcag gtgtggtttg
     3061 tcgttgtgca tataaatttt tagaaagtaa ttttatttcg gattacgtaa tttactgtgc
     3121 tttgcactgt agccaaaata aataacgaaa cctatagcca gccacattag taagcgtgac
     3181 caattaggcc aacccaagcc gaatatcatg gctccacaaa caataattcc taagatggga
     3241 actaatggaa ctaaaggtgt tctgaaagta cgttttgcat cgggattggt tttacgcata
     3301 atgattacac cggctgcaac cagcataaat gcaaacagtg ttccaatgct tgtcatatca
     3361 ccaacaatat cacccggtac aaatgcagca aaaagtccga caaaaacaaa gagaattaaa
     3421 ttcgatttat aaggtgttcg gtaaacagga tgtagttctg aaaaaacttt aggcaacaat
     3481 ccatcacgac tcatagaata aaatacacgt gattgtccta aaagcataac taaaattaca
     3541 gaggagaatc cggcaagaat agcaacggtt accaattttg ctaaccagtc gtagccatgc
     3601 atataggttt gaatggcata agtcactgag gcttcacggc cttgtgttct gaaatcttct
     3661 actgaggcaa ctcctgttag cacatgagca aacagaatgt aaaggacagt acaaattatt
     3721 aatgaaccta aaattccttt tggcatatca cgaccgggat tttttgcttc ttgtgcagca
     3781 gtgctcacgg catcaaaacc aatgaatgca aagaatacca caccggcagc accaagaatt
     3841 cccaatattc caccatagtg atgtggcaca cccatgtggt cggtgaaagt gcttgcagca
     3901 ggtatataag gcgtgtgatt agcaggatta ataaattgcc agccaaaaac aataaagagt
     3961 attacaatag ccactttagt aactacaatg ataccattta cgaaggcact ttcttgagta
     4021 ccttttatta agagtaagga aagtaatatg agaattacca atgcaggaac attcataata
     4081 ccataaacac catctaaact atgttcaaaa ggtgaatggc accattcata aggtatgtgc
     4141 atgcccagat aattcaacaa tttgttcagg tattcgctcc aggcaatact cactgttgct
     4201 gcaccaagtg catattcaag cacaagatcc catccgatta tccaggcaat aaattcaccc
     4261 attgtagtgt aactataagt ataggcacta ccggcaatag gtatcatact tgcaaattct
     4321 gcatagcaca atcctgcaaa ggcgcaaccc aatcctgcaa taacaaaagc taatgtaaca
     4381 gccggcccag cattgtttgc agcagcagca gcagtgcgca caaatagtcc tgcaccaata
     4441 atagcaccaa taccgagtgc aataagactg tttgcagtaa gcgttctttt taaacctttt
     4501 tcgctgtctt ctgcttcagc taataaacta tcgagaggtt tttttacatg tattcctttg
     4561 gacatacctt tgatcttgat ttttagtgga gttcaaac
//
LOCUS       DAORQT010000116         4566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3318_length_4566_cov_5.056528, whole genome shotgun sequence.
ACCESSION   DAORQT010000116 DAORQT010000000
VERSION     DAORQT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_4566_cov_5.056528"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..701
                     /locus_tag="PK134_05200"
     CDS             171..701
                     /locus_tag="PK134_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB38279.1"
                     /translation="MTTTLKATFLSLAIIGTAFFAQAQKPVEGIAIGNKAPELAFTSP
                     EGKTIPLSSLKGKIVLIDFWASWCGPCRMENPNVVAAYNKYKDKKFKGAKGFTVYGVS
                     LDQDKNRWMNAIKQDKLDWPSHVSDLAGWNSKAAQIYRVNSIPANWLIDGNGYIVGSN
                     LRGPALDAAIEKLLAQ"
     gene            876..1430
                     /locus_tag="PK134_05205"
     CDS             876..1430
                     /locus_tag="PK134_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRB38280.1"
                     /translation="MKKENEIISNEESSNPEEIMPKQEVIDETSVSEEIQNPLELLKE
                     ELSVANDKYLRLYSDFENYKRRLAKERIELIKTAGADVITSLLPIIDDFERAMKAQEN
                     TSDVTALKEGIQLIFQKLNNTLTQQGLTAIESINKPFDTDLHEAITSIPADDDKKGLV
                     VDEIEKGYMLNDKVVRHAKVVVGS"
     gene            1426..2577
                     /gene="dnaJ"
                     /locus_tag="PK134_05210"
                     /pseudo
     CDS             1426..2577
                     /gene="dnaJ"
                     /locus_tag="PK134_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
     assembly_gap    3002..3061
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3245..4048)
                     /locus_tag="PK134_05215"
     CDS             complement(3245..4048)
                     /locus_tag="PK134_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB38281.1"
                     /translation="MKHQGGSLAGSGLGGGAGIWLATHYNLTLAGIVLCMVSVLFALT
                     MFLIKDIPFKKQKSIILEIKGMGINIFSMIKLPVALFVMILILMPIGTGAMSNLWSAV
                     AQDWKADADTVALVTGILSGIVSDVGCIAGGFISDRWGIWNAYLGIGVICALVTVLMA
                     FMPYTPPAYISGVLVYGFCMGLMYAAYTAVLLFVIGKSHSATKFSLMSSLGNMPVVYM
                     TTFNGWAHDKYGSKFMLSAEAFIGIIFIVIFYLILLRMKKKNMIPLIVD"
     gene            complement(4029..4394)
                     /locus_tag="PK134_05220"
     CDS             complement(4029..4394)
                     /locus_tag="PK134_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38282.1"
                     /translation="MGISQGFITVTLPFMLTQNGFSVALTAGIVAIGISANLWRFVWG
                     PIVDLSLSLHKWYWLSMSASIASLLLICSIPFTIKGETLLSVIVFISQVAATIALLPL
                     NGIMAKQIHENKKGEASGW"
BASE COUNT         1522 a          752 c          973 g         1259 t
ORIGIN      
        1 atattgaaca aatagaccgt ggttatgaaa gaatagatga acgattgata aaacttggtg
       61 cccacatcaa aaggatttaa aacaatttaa tattttagcg tttaataaaa atcaaatgaa
      121 ggcctgccac tgtggtacga tagttggcag ccataaacaa aattaaaaat atgacaacta
      181 cacttaaagc aacattctta tcgctggcaa tcatcggcac tgcatttttt gcacaggcac
      241 aaaagccggt tgaaggcatt gccattggca acaaagcacc ggagctggca tttacatcac
      301 cagaaggaaa aactattccc ctttcatcac tgaaaggaaa aattgtgcta atagattttt
      361 gggcatcatg gtgcggccca tgccgtatgg aaaatcctaa tgtggtagct gcctataata
      421 aatataaaga caaaaaattt aaaggtgcca aaggttttac tgtttatggc gtatcgctcg
      481 atcaggataa aaatcgttgg atgaatgcta ttaagcaaga caaattagac tggcctagcc
      541 atgtgagtga tcttgcagga tggaatagta aagcagcgca aatttatcgt gttaactcaa
      601 ttcctgccaa ctggcttatt gatggaaacg gatatattgt gggtagtaat ttaagagggc
      661 ctgctttaga tgcagcaatc gaaaaattat tggcacaata aataatattt tattatcgaa
      721 gaatgacaac agccggaaat tccggctgtt gtcattttac ctcctgtttg acagttgcag
      781 ccgggtaaaa gctatctttg cctgccattt tgaccccaat tgttaaatgg caatgttttt
      841 gaaaatcagc gatgaaattg tttaagcgta gaaaaatgaa aaaggaaaac gaaattatat
      901 ctaatgaaga atcctcaaac cctgaggaaa taatgccgaa acaagaagtt atagatgaaa
      961 cttctgtaag tgaggaaata caaaatcctc ttgaactatt gaaagaagag ttgtctgttg
     1021 ccaatgacaa atatctgaga ttgtacagcg actttgagaa ttacaaacgc agattagcga
     1081 aagagcgtat tgaactgata aaaactgcag gtgcagatgt gattacatca ctgctaccaa
     1141 ttatagatga ttttgagcgt gcaatgaaag cacaggaaaa tacttcagat gtaactgctt
     1201 taaaagaagg cattcaatta atttttcaga agcttaacaa caccttaacc caacaagggt
     1261 tgacagcaat tgaatcaata aacaaacctt ttgataccga cctgcacgaa gctattacaa
     1321 gcattcctgc cgatgatgat aaaaaaggat tggtagttga tgaaattgaa aaaggctata
     1381 tgctgaatga taaagttgtg cgtcatgcca aggtagtggt gggaagttaa tagaaaacat
     1441 tattacgaaa tattaggtgt tggacgcagt gcttcggaag ctgaaataaa aaaagcttac
     1501 cgtcagatgg ctattaaata tcatcctgac aaaaatccgg gcaataaaga agccgaagaa
     1561 aaatttaaag aagctgctga ggcttatgaa gtgctgagta atgcagagaa aaaaagccgc
     1621 tacgatcagt ttggtcatca ggctatgggc aacggtggtt atggtggcgg acagcacatg
     1681 aacatggaag atatcttcag tcagtttgga gatatttttg gcggacacaa cccttttgag
     1741 agtttctttg gtggtggtgg cggacagcgt ggaggccgca gagttaaccg tggctcaaac
     1801 ctgcgtatta aagtaaaact tacacttcag gaaattgcca acggtgttga gaagaaatta
     1861 aaagtaagta agaaagttgc ctgccatacc tgtcatggta gtggtgcaca gggcaacaat
     1921 gcatttacaa catgcagtgc ctgcaaaggt tcgggacagg tgagacgtgt tacgaatact
     1981 attttaggtc agatgcaaac agttactacc tgtaatgttt gtggtggcga aggacaggtt
     2041 attgccaata cctgccgcac ttgtcatggt acgggtgttg agcagggaga agatatgatt
     2101 acagtaccca ttcctgcagg tgttgcagat ggtatgcaac ttaacgttag cggaaaaggt
     2161 aatcttggcg agcgcaatgg cattgccggt gatttgctta tagtgataga agagattgaa
     2221 gatccaaagc tgaaacgtga tggtgataat ttgctgttcg atttatacat cacatttatt
     2281 gatgctgcat taggcactca ggttgaaatt ccaactgttg acggtaaagc gaaaattaaa
     2341 attgatgccg gcacacaagg cggcaaagtg ttgcgattga aaggaaaagg actaccaaga
     2401 gtaaacagtt atcataaagg cgacctgctt gtgaatatta acgtgtggac accacagcag
     2461 ctttctgcag aagaaagaaa gatgcttgag aagctacgcg actcagaaaa ttttaaacct
     2521 aatccgggta agggtgataa aagctttttc gaaaaaatga aagagttttt taattaggca
     2581 ttgaggttgg aaggttggat ttgataattt gataatttga aaatgtgttg atttgataat
     2641 ttgataattt gaaaatgaga taatcagcca cggctgacag gtttgaaaat gggagtttgg
     2701 aaagtttgga aagtttggaa ggttgggaag tttggaaagt tggaaagttt ggaaagttgg
     2761 tgtttacata gttatttaat gttttcaaga agcctgcgca cacccatagc cataagaggc
     2821 tttgcttttt acaaaatgcc taaagggcgc tgacacacga taagcaatta aactgtaaac
     2881 tttatttagg aaaagacact gacatttgcg gtgctgaggt tggaatttgc ggtgctgagg
     2941 atggctattt atgtgctatg ttaagatatt tatgataata gttagaaaaa agaagatgtt
     3001 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 ntgcgtttta caaaatgcct attgcggtca gtcacacgta aatttaggac actcgaatgt
     3121 ttttttaggt gctgagtctg tcatttgcgg tgctgaggtt ggagtttgcg gtgctgaagg
     3181 tttttaattt agtttgattt tcagctatat atacacaagg ttacacaact tgccatttac
     3241 ccgattaatc aacaataaga gggatcatgt ttttcttttt catacgaagt aatataaggt
     3301 aaaaaatgac gataaatatt attcctataa atgcttcggc agatagcata aatttacttc
     3361 catatttatc atgagcccaa ccattgaaag tggtcatata caccacaggc atattgccta
     3421 aagatgacat taaagaaaat ttagttgctg aatggctttt gccgattaca aaaagaagaa
     3481 cggcagtgta tgcagcatac attaatccca tacaaaatcc ataaacaagc actcctgaaa
     3541 tatatgccgg tggtgtgtag ggcataaaag ccatgagtac agttaccaat gcacaaataa
     3601 ctccaatgcc aagatatgca ttccaaattc cccaacggtc tgaaataaaa cctcctgcta
     3661 tgcaacccac atcacttaca atgcccgata agattccggt gacaagagca accgtatctg
     3721 catctgcttt ccagtcctga gccactgctg accatagatt ggacatggca ccggtgccaa
     3781 tgggcatcag tataagaatc attacaaata aagccacggg caattttatc atagaaaaaa
     3841 tgtttatccc cattccttta atctcaagaa taattgattt ttgttttttg aagggaatat
     3901 ctttgatgag aaacattgtt aatgcaaaca aaacagacac catacataaa acaataccag
     3961 caagggtaag attatagtgt gtggcaagcc atattcctgc tccacctccc aaacctgaac
     4021 cggcaaggct accaccctga tgcttcacct tttttatttt cgtgaatttg ttttgccata
     4081 atcccattaa gaggtaatag agctatagtg gcagctactt gcgatataaa gacaattact
     4141 gaaagcaatg tttctccttt tattgtaaac ggaattgaac aaataagcaa taatgaagca
     4201 atggaagctg acatgcttag ccaataccat ttgtgtaagc taagagataa atctactatt
     4261 ggcccccaaa caaaacgcca taggtttgct gaaattccga tagcaacgat acctgcagtt
     4321 agtgcaactg aaaagccatt ctgtgtgagc ataaatggta atgtgaccgt tataaagccc
     4381 tggcttatac ccattggcat gattagaaaa aatatataaa atggttttgt gtagtgttgg
     4441 ggcatgtggt tgaattttaa attttattca gtctcttaat ttttgatttc agatattaat
     4501 ttttgtgagt ctattaagac aacaataaaa gtgcaacatt aaaaatcaag tgctaatgaa
     4561 attaat
//
LOCUS       DAORQT010000117         4491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3410_length_4491_cov_6.867448, whole genome shotgun sequence.
ACCESSION   DAORQT010000117 DAORQT010000000
VERSION     DAORQT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_4491_cov_6.867448"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   19..496
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19..55
                     /rpt_unit_seq="atttcagagcaatatccatcagaataaggattgaaac"
     gene            complement(614..1360)
                     /gene="istB"
                     /locus_tag="PK134_05225"
     CDS             complement(614..1360)
                     /gene="istB"
                     /locus_tag="PK134_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRB38283.1"
                     /translation="MNTNQTIAQMRTLNLTGMARAYQSLLQLPLNEHPTADMLIAQLI
                     EAEVLERNHRKMLTTIKAAKFRYQAAIEEIDFLAQRNLDKNTILRLSDVSFIKRAENV
                     LITGATGCGKSYIASALGFKACQMGNRVAYFSMTKLLQRLQMAKADGSYIKELAKIEK
                     TQLLILDDWGLQTLDTQAKLTLLQIIEDRHGKAATIITSQLPVPQWFDYLNEPTLADA
                     ILDRILQQAHRIDLKGESMRKRKKINEVSS"
     gene            complement(1406..2095)
                     /locus_tag="PK134_05230"
     CDS             complement(1406..2095)
                     /locus_tag="PK134_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38284.1"
                     /translation="MVTPYGSRTTPLQGKTESRQSLFDDIERVTLQILPAVPYELRWF
                     REAKVHPNCHVKLSEDKHYYSVPYKHVGMTVQIRYTQQTVEIYADYQRIAIHERIIAK
                     NGYTTLKEHLHPSHQWVMNWSPEFFQTQAAKIGQHTLKAIQQLILSRKYPEQAYKSCA
                     GLLALAPKVGNQRLENACERALAYEYISAKLIQSILDRELDKVPIREEELTQVSVIPL
                     HPNIRGAGNYQ"
     gene            2145..2417
                     /locus_tag="PK134_05235"
     CDS             2145..2417
                     /locus_tag="PK134_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB38285.1"
                     /translation="MKQSKFSDAQIVAILGSVGKDKSVENVCREHGISPATFYTWKSK
                     YGGMGTDELKRMRELESENARLKKLLADKSLDYDILKEGYELLKKL"
     gene            2414..3265
                     /locus_tag="PK134_05240"
     CDS             2414..3265
                     /locus_tag="PK134_05240"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB38286.1"
                     /translation="MSSTTHQHAVNQISTKKEASVRRCCRVLGQRKASIYYKHKRKNE
                     DAQIAAMLKGKAIEQPNWGFRLLFHWCRNQGKPWNKKRVYRIYKAEKLNLRSPKARKK
                     IKRVAINPLPAEQINQGWSMDFLSDVLLSDERKVRVLNVLDECSRKVLLSFAAPSIKA
                     RKLVKLLQELITQKGKPDYIRCDNGPEFISRVLEKFTQENGIEIRFSQPGKPTQNGLV
                     ERLNGTQRKECLNLKYFKTVQEVQRDLDIWWNSYNFDRPHSALGYLTPQQFIDKNQNL
                     YFKAVAA"
     gene            complement(3246..4172)
                     /gene="istA"
                     /locus_tag="PK134_05245"
     CDS             complement(3246..4172)
                     /gene="istA"
                     /locus_tag="PK134_05245"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRB38287.1"
                     /translation="MANQRITMQKLQKLIELHLSGKSQRQISGLLGIHRRTISNYLTA
                     LSRYSHGDLLTLQGLDESSLWGIVNTPLVSPERVTDIISLFPSYEKRLAQVGMTRYLL
                     WEDYRGHHEGKLSYSRFCRVFAKWKKNSKISLHIEHKAGDKLFIDFAGKKLYLTDPQS
                     NETTAVEVFVAVLGYSQLTYCQVIPSQKKPDFLSALANALAFYGGVPQAIVPDNLKSA
                     VTKADRYEPDLNESLADFADHYQTTIYPTRSRRPKDKALVEKTINIIYTRIYAALCDK
                     VFSTPAQLNEAIRERLTKHNLTLSCSPKPQPL"
BASE COUNT         1378 a          851 c         1003 g         1259 t
ORIGIN      
        1 taattcagcc tgtttcggat ttcagagcaa tatccatcag aataaggatt gaaacggcag
       61 tattaaatcg tctccatatt tttctctcat catatttcag agcaatatcc atcagaataa
      121 ggattgaaac aataaatttt tctaccctac acaatgactt catctaattt cagagcaata
      181 tccatcagaa taaggattga aacattacga agccggagcg ggattcgaac ccgcaacctt
      241 atttcagagc aatatccatc agaataagga ttgaaacacc ggtagtcggg tcaaagttag
      301 caacgttcga catatttcag agcaatatcc atcagaataa ggattgaaac attgtgaatg
      361 gggtggagta tcacagctat actattattt cagagcaata tccatcagaa taaggattga
      421 aacctgtacg tgcctgtggc cgatgactac gaagctgtaa tttcagagca atatccatca
      481 gaataaggat tgaaactcaa actcagggcc gaaactttca tgaatgtaaa atggcgtgaa
      541 agtgtaccgg taaatggcgc aaaagtgtac cggaattaga agcagagaga acgtagatac
      601 aaaggttaaa aaattagctt gatacttcgt tgattttctt acgttttctc atagactctc
      661 ctttcaagtc aatgcgatgg gcttgctgta aaattctgtc caatatggca tcggctaatg
      721 tgggttcgtt cagatagtcg aaccattgcg gcacgggaag ttgtgaagtg atgatggttg
      781 ccgcttttcc atgacggtct tcaatgattt gcaaaagtgt caatttagct tgtgtatcca
      841 gcgtttgcag gccccagtca tctaaaatca gcagttgtgt tttttctatt ttggcgagtt
      901 ctttgatata agagccatca gcttttgcca tttggagtct ttgcagcagt ttggtcatag
      961 aaaagtaggc aacccgattg cccatttgac aagccttgaa gcccagagcc gaagcgatat
     1021 agctttttcc acagcctgtt gctcctgtaa taaggacatt ttcagcacgt ttgatgaaag
     1081 agacatcact aagcctaaga atggtatttt tgtccaagtt tcgttgggcc aggaagtcaa
     1141 tttcttcgat agctgcctga tagcggaact tagcggcttt gatggtggta agcatcttac
     1201 ggtggttgcg ttccaagact tctgcctcaa tcaattgggc aataagcata tcagctgtag
     1261 gatgttcatt gagtgggagt tgaagcaaag actgataagc ccttgccatt cctgtcagat
     1321 tcagcgttcg catctgagcg atggtttgat ttgtgttcat tggataattg tgtttggttt
     1381 tggattggat cagagccgga aaaatttact gataattacc ggctcctctg atatttggat
     1441 gcagtggaat gacagagact tgcgtaagtt cttcctctcg tataggtact ttatccagtt
     1501 ctctatcaag gatactttgg atcagtttag ccgagatata ttcataggcc aatgcccttt
     1561 cacaggcgtt ttctaagcgt tgattaccca cttttggggc caaagccaag aggcctgcac
     1621 aagatttata agcttgttcg ggatactttc gtgagagaat cagttgttga attgctttca
     1681 gagtatgctg tccgattttg gcagcctgag tttgaaaaaa ttccggtgac caattcataa
     1741 cccattgatg tgatgggtgc agatgctctt tcagggtagt gtaaccattt ttggcaatga
     1801 tacgctcatg gatagcaata cgctgataat cagcatagat ttcaactgtc tgctgagtat
     1861 aacgaatctg aacagtcatc ccaacgtgct tatagggaac cgaataatag tgcttgtctt
     1921 cggagagttt aacgtgacaa ttaggatgta ctttagcctc tctgaaccaa cgaagttcat
     1981 acggcacagc tggcaaaatt tgcagagtaa ctcgttctat gtcatcaaaa aggctttgac
     2041 gactttccgt tttaccttgt agaggagttg tcctgctccc gtaaggtgta accatttaaa
     2101 attggaggga atttcttagg tttcacaatc aaaatggtaa aacaatgaag caaagtaaat
     2161 ttagcgatgc tcaaatcgta gcgatattgg gtagtgtagg caaagacaag agtgttgaaa
     2221 atgtatgccg cgaacatggt atctcaccag caaccttcta tacttggaaa agtaagtatg
     2281 gaggcatggg cacagacgag ctcaagcgca tgcgagaatt ggaatcagaa aatgcaaggt
     2341 taaaaaaact gttagcggac aaaagccttg actatgacat actcaaggag gggtacgagt
     2401 tactaaaaaa gctataagct ctactactca tcagcatgca gttaatcaaa ttagcacaaa
     2461 aaaagaggcc tctgtgaggc gatgctgccg agtattaggg caacgtaaag cgagtattta
     2521 ttacaaacac aaaagaaaaa atgaagatgc tcaaatagct gcaatgttaa aaggtaaagc
     2581 tattgagcaa cccaattggg gttttcgttt gttgtttcat tggtgtcgta atcagggcaa
     2641 accatggaat aaaaaacggg tttatcgaat ctataaggct gaaaaattga atttaagaag
     2701 ccccaaagca cgcaaaaaaa tcaaaagggt agccataaac ccattacctg ccgaacaaat
     2761 aaaccaagga tggtcgatgg actttttaag cgatgttctt ttaagtgatg agcgtaaagt
     2821 gagagttcta aacgtacttg acgagtgttc aagaaaggta cttttatctt ttgctgcacc
     2881 aagcattaaa gcaagaaaac tcgtaaagct attacaagaa ctgataactc agaaaggtaa
     2941 acctgactat atcagatgtg ataatggacc cgagtttata agtcgtgttt tagaaaaatt
     3001 tacacaagaa aatgggattg aaattcgatt ttcacaacca ggaaaaccga cccaaaatgg
     3061 cttggtagaa cgattgaacg gtacacaacg aaaagaatgt ttaaatctga aatattttaa
     3121 gacagtacaa gaagttcaac gggatttgga catctggtgg aacagttata actttgatag
     3181 accgcattca gcactgggat atttaacccc acaacagttt attgacaaaa atcaaaatct
     3241 ctactttaaa gcggttgcgg cttaggggag caggacagag taaggttatg cttagttaag
     3301 cgctcccgaa tcgcttcatt gagttgtgcc ggcgtgctga atactttgtc gcataaagct
     3361 gcataaatgc gcgtgtagat aatattgatt gtcttctcca ctaaggcttt atccttaggg
     3421 cgacgactgc gagtggggta aatggtagtc tgataatgat cggcaaaatc ggctaaactc
     3481 tcgttgaggt ccggttcata gcgatcggct ttggtaacgg ctgacttgag attatcggga
     3541 acaatggctt gcggcacacc tccataaaag gccaaagcat tagccagagc tgagagaaag
     3601 tcaggcttct tttggctggg aatcacttgg cagtaagtca gttgagagta gcccaaaaca
     3661 gctacaaaga cttctacagc agtcgtttca ttgctctgtg ggtcagttag atagagcttc
     3721 ttgccagcaa aatcaatgaa taacttgtca ccggccttat gctcaatatg tagacttatt
     3781 ttgctgtttt ttttccattt ggcaaagacg cgacagaaac gggaataact cagtttaccc
     3841 tcgtgatggc cacggtagtc ctcccataac aggtaacgcg tcatgcctac ttgagccaat
     3901 cgcttttcgt aggaagggaa aaggctaatt atatcagtaa cacgctcagg acttacaagg
     3961 ggtgtattga caattcccca aagagatgac tcgtcaagac cttgtagagt taataaatcg
     4021 ccatgagaat atctgctcaa agcagtcagg taattgctta ttgtgcgacg atgtattcca
     4081 agtaagccgg aaatctgtcg ctgactctta cccgacaggt gcaattcaat cagtttttga
     4141 agtttttgca tggtgatacg ttggtttgcc atagctcgaa aattgaagga tttatcaatt
     4201 ctcgaaagct atgaatcaac gctatctctt gctaatttgg gtggtacact ttcgcgccaa
     4261 tcggtggtac actttcatag gaatttacac atgaacgccg atcccatatt tcagagcaat
     4321 atccatcaga ataaggattg aaacatgtct tcgtacaaaa taccggtagt cagcgcagta
     4381 catttcagag caatatccat cagaataagg attgaaactc ttacgcaggc ttgaagtaat
     4441 tcagcctgtt tcggatttca gagcaatatc catcagaata aggattgaaa c
//
LOCUS       DAORQT010000118         4420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3481_length_4420_cov_6.232073, whole genome shotgun sequence.
ACCESSION   DAORQT010000118 DAORQT010000000
VERSION     DAORQT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_4420_cov_6.232073"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PK134_05250"
     CDS             <1..1455
                     /locus_tag="PK134_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38288.1"
                     /translation="VVLLLLCSSGILYSVFQSSDYLARAAGLSFKVASFGPFSPQSLI
                     SLMFPLASARDSEFFGTDISMSSVFVGMLPLAGFILFIIRPKSRSAWLILAATIVMLF
                     ASFGSYTPVFGWFYKTFPMLDLFRFPSVYRLFFITGILLRSGIALSAPDAFSRLKLPF
                     ATLGGIAAVATIFFWLKNTNEWNFKPWHDAVNNISFTQAIAFQGFIQLLFIVMMLLAL
                     LIKDRTKLLMAMITLSAVNVIVAAQLNIPVTVTYNLKTSEVAKEVKEKAVADFPVPEV
                     RPVVLNNDSGGWVSPFWRNLNLFKKQPAWDGYNSFQLKNYVRFVDDESLRQRQISNNW
                     IYFADTVIAYSEEPIIVAPASLHNVCYVPANEKDLLNIQPASGNSISVKTFIPGKIIV
                     EAKTETSSALTLMQQHYHGWKIYDNDKEVKPVVTDYLFMTIPLAQGTHKIEYRYENKP
                     IHNALWLSGVTLIVLIGFSLYAFFSKNKLQALPK"
     assembly_gap    1474..1494
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1585..2082
                     /locus_tag="PK134_05255"
     CDS             1585..2082
                     /locus_tag="PK134_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRB38289.1"
                     /translation="MSETKLKEKLNKEINTFFHKSRDWDIQFKTTKKITYEDFLSDKI
                     LMIAAIRAGIPYSLFQLIKGFTPFTEYDWADFLDISTKSLQRYHQSSQHYFKPIHSEK
                     IIEMAEVTKVGLEVFEDMDKLKLWLNTPNYALGKIKPIVLLKDSYGKELVMSELIRIN
                     HGILV"
     gene            2086..2544
                     /locus_tag="PK134_05260"
     CDS             2086..2544
                     /locus_tag="PK134_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRB38290.1"
                     /translation="MEAFRLTRAKFANLLSGKGAAIKGARWNSIGVELIYTASNRSLA
                     MAEVAVHFTLATLPADYMMVTINIPDSVSVQEMKEKELPTDWRNFPHPISTQIIGDRF
                     VAENKFCVLRIPSVVTLGDYNLLINPKHRDFVKIKMKSVEKFPFDKRILR"
     gene            complement(2555..3697)
                     /locus_tag="PK134_05265"
     CDS             complement(2555..3697)
                     /locus_tag="PK134_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRB38291.1"
                     /translation="MALIAVNTRMLLKDKLDGTGWFAFETLKRIVIQHPEHRFIFFFD
                     RKFNSEFIFSNNIMPVVIYPQARHPWLWYTWFEISLHHAFKKYKPDLFFSPESFICSK
                     ATVPQVAVIHDLNFEHFPQYMPTQVARYYSKWSRIAAQNAQRIGTVSEFSRKDIVSTY
                     EINIEKTDVLYNGIHDMYKPLSVAEQEQVRNQYTAGSRYIIYTGSLHQRKNTANMVRA
                     FAQYKKQYSGELKFVLVSDRSKMEADLQLALSETGIESDLIFTGRLSAKALAQLTASA
                     EAMMYVSFFEGFGIPMIEAMRCGVPVITSNVSCLPEIAGNAALCVDPHSVNAITVALE
                     QVLQHQEVAEQLKQKGKIRQQDFSWDKTAELTWQSLQLVLAEKSKG"
     assembly_gap    3895..3960
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3961..>4187
                     /locus_tag="PK134_05270"
     CDS             <3961..>4187
                     /locus_tag="PK134_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38292.1"
                     /translation="CATDQAVYFIDKSDNSVQRYSKINGLSDVGIRLMDYSHTENVVF
                     VGYVNSNIDLISSTGIINISDVERRTATGDK"
     assembly_gap    4188..4197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4198..>4420
                     /locus_tag="PK134_05275"
     CDS             <4198..>4420
                     /locus_tag="PK134_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38293.1"
                     /translation="VAFNGKFAYLATGFGVLALDLIKAEIKETYVIGTTGSEVPVYSV
                     TTDGTYIYAAIAGGILRGEISNPALNNFAT"
BASE COUNT         1307 a          806 c          822 g         1388 t
ORIGIN      
        1 gttgtgttgt tattactgtg ttcatcaggt attttgtaca gtgtttttca aagttcggat
       61 tatcttgcac gggcagcagg tctttcattc aaagtagcct cgtttggtcc cttttcacca
      121 cagtcgctca tatcgctaat gtttccattg gcttctgctc gcgacagtga attttttggt
      181 acagatatca gtatgtcctc tgtgtttgtt ggtatgttgc cgttggcagg gtttatactc
      241 tttatcatca gacccaaatc gcgctctgca tggcttattc ttgcagcgac aatcgtcatg
      301 ctctttgcat catttggaag ctacaccccg gtttttggct ggttttacaa aacttttccc
      361 atgcttgatt tattccgatt cccctctgta tatagattgt tttttattac cggaatattg
      421 ttgcgttcgg gcattgcact atctgctccc gatgcattca gccgccttaa gttacccttt
      481 gccaccttag gtggcattgc cgcagtagct acaatttttt tctggttaaa gaatactaat
      541 gaatggaatt ttaaaccctg gcatgatgct gtaaacaaca tttcgtttac tcaggcaata
      601 gcttttcagg gttttattca gttgcttttt attgtgatga tgttgctggc tttattaatt
      661 aaagatagaa caaagcttct gatggcaatg ataacattgt ctgcagttaa tgtaattgtt
      721 gcagcacaat taaacattcc tgtaacggtg acttacaatc ttaaaacttc tgaagtagca
      781 aaagaagtaa aagaaaaagc tgttgcagat tttcctgttc ctgaagtgcg gcctgttgta
      841 ctcaacaacg actccggtgg gtgggtaagt cctttttgga gaaatcttaa cctgtttaaa
      901 aaacaaccgg catgggatgg ctataattct ttccagctga aaaattatgt acgctttgtt
      961 gatgatgaat cgctcaggca aagacagata agcaacaact ggatttattt tgccgacact
     1021 gttattgctt attctgaaga gccaatcatt gtggcacctg catcgctgca taatgtttgt
     1081 tatgtgcctg caaacgaaaa agacttgttg aatattcaac cagcatcagg caatagcatt
     1141 tctgtaaaga catttattcc gggaaaaatt attgttgaag caaaaacaga aacatcttct
     1201 gcattaacac tgatgcagca gcactatcat ggatggaaaa tttatgataa tgacaaagaa
     1261 gttaaacctg tcgttaccga ttatttattt atgacgattc ctttagcaca aggtactcat
     1321 aagatagaat accgttatga aaacaaaccg atacataatg ctttgtggct ttcgggagtt
     1381 acgctaattg tactgatagg cttttcactt tatgcttttt tcagcaagaa caagttgcag
     1441 gctttgccaa agtaatgtta ttgaatatta tttnnnnnnn nnnnnnnnnn nnnncaaacc
     1501 tgtttgttaa tttctcatta ccgcaaatag aattaattga cagttacaga aaaatgtctt
     1561 atatttgctg acaaatgtct tgttatgtca gaaacaaaat taaaagagaa acttaataag
     1621 gagataaaca ccttctttca taagtcgaga gattgggata tacagttcaa gacaacgaag
     1681 aaaataacgt atgaagattt tctttcagat aagattttaa tgattgcggc aataagagcc
     1741 ggcatacctt attcgttgtt tcaattaata aagggtttta caccttttac agaatatgat
     1801 tgggctgatt ttcttgatat atctaccaag tcattacagc gatatcatca atcatctcaa
     1861 cattacttca aaccaataca ctcagaaaaa ataatagaaa tggcagaagt aactaaagtc
     1921 ggacttgaag tttttgagga tatggataaa ctgaaacttt ggttaaatac tccaaattac
     1981 gccttaggaa aaataaaacc tattgtatta ttgaaagact cttatggaaa ggaattagta
     2041 atgtcagaat tgataagaat caaccatggt attcttgttt agtctatgga ggcattcaga
     2101 ctgaccagag ccaagtttgc aaatttatta tccggaaagg gggctgctat taaaggcgca
     2161 aggtggaatt ctattggtgt agaattgatc tatacagctt caaaccgctc tttagctatg
     2221 gcagaagttg cagtacattt tactttagca acattacctg cagattatat gatggtaaca
     2281 ataaatattc cggatagcgt ttctgttcag gaaatgaaag aaaaagaact gccaaccgat
     2341 tggcgaaact tcccgcatcc aatatcaact caaattattg gtgatagatt tgttgctgaa
     2401 aataagtttt gtgttttacg aattccctca gtggttactc tgggagacta taatttactc
     2461 atcaacccaa agcatcgtga ctttgttaaa ataaaaatga aatctgtaga aaaattccct
     2521 tttgataaga gaatactcag ataaagacta actcttatcc tttgcttttt tcagcaagaa
     2581 caagttgcag gctttgccac gttagttcag cagttttatc ccaagaaaaa tcctgttgtc
     2641 tgattttgcc tttttgtttt agctgttcgg caacttcctg atgttgtaat acttgttcca
     2701 aagcaacggt aatggcattt acgctgtgtg ggtcaacaca gagagctgca ttgcctgcaa
     2761 tttcaggaag gcaacttaca ttggaagtga ttacaggtac accacagcgc atagcttcaa
     2821 tcatcggaat accaaatcct tcaaaaaacg aaacatacat cattgcctct gccgatgcag
     2881 ttagttgtgc caatgctttt gcagaaagcc ttcctgtaaa aatcaagtcg ctctcaattc
     2941 ctgtttcaga aagtgcaagt tgcaaatcag cttccatttt acttcgatcg gacactaata
     3001 caaattttaa ttctccgcta tattgctttt tatactgtgc aaaagcacgc accatgttag
     3061 ctgtgttttt tcgctgatgc aatgagccgg tgtaaattat atagcggctt cctgcagtat
     3121 attgattgcg tacttgctct tgttctgcta ctgataatgg cttgtacata tcatgaatac
     3181 cattatacaa cacatcggtt ttttcaatgt taatttcata ggtgctcaca atatcctttc
     3241 gcgaaaactc agacacagta cctattcttt gtgcattttg tgcagctatg cgcgaccact
     3301 tactatagta acgagcaact tgtgttggca tatattgtgg aaaatgttca aagtttaaat
     3361 cgtgaattac tgctacctgt ggtacagtag ccttactgca aataaaactc tcgggactga
     3421 agaatagatc cggtttatat tttttaaaag cgtggtgaag gcttatttca aaccaggtgt
     3481 accaaagcca cggatgacgt gcctgcgggt aaatgactac cggcataata ttattgctga
     3541 aaataaactc agaattaaat ttacggtcga aaaaaaagat aaaacgatgt tcgggatgct
     3601 gaatgacaat acgcttcagg gtctcgaatg caaaccatcc tgttccatct aacttgtctt
     3661 taagcaacat tctggtgttt accgctatca gcgccattgc gattatttat atttttgtag
     3721 tgcaaaagta ttttaaatag aaactcatag caggccataa aataaattcc ctcatgttga
     3781 gttatatatt ccgtatcccg aaccgtatgt acaaacagtt gtccattgcc ttttttctgt
     3841 tgctctctgc agcacaatcc atttcgcaaa atctaccatt aggctcatgg cgcgnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 ttgcgccaca gatcaggctg tatattttat tgataagtcg gacaacagcg tacaacgtta
     4021 ttcaaaaatt aacggacttt cagatgtagg tataaggctg atggattaca gccatacaga
     4081 aaatgtggtg tttgtaggct atgtaaattc caatattgac ctcatatcaa gtacaggcat
     4141 tattaatatt tctgatgttg aaagaagaac agctaccggt gacaaggnnn nnnnnnnatg
     4201 tagcatttaa cggaaagttt gcatatctgg caacaggctt tggagtttta gcactcgatt
     4261 taattaaagc tgaaataaaa gaaacctacg ttatcggaac cacaggaagt gaagttcctg
     4321 tgtattcagt taccactgat ggcacctata tttatgctgc cattgcaggc ggaatcctta
     4381 gaggagaaat aagcaatccc gcactcaata attttgctac
//
LOCUS       DAORQT010000119         4395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3520_length_4395_cov_6.948387, whole genome shotgun sequence.
ACCESSION   DAORQT010000119 DAORQT010000000
VERSION     DAORQT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3520_length_4395_cov_6.948387"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PK134_05280"
     CDS             complement(<1..820)
                     /locus_tag="PK134_05280"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRB38294.1"
                     /translation="MKKIVVFLVALMLTNIINAQDLKPFKSDKELWGYKDATGKEIIP
                     ARYNFAWSFTEGIARVRNKKMKYGFIDVTGKEVIPIKYEDAKDFYEGLAAVKSDDKCG
                     FIDKTGNVIIPLRYDEVGNFSEGLASARSGAVRGGKYNTLSGGKYGFIDKSGNEIIAF
                     KYDGAGDFSEGFAYVKVGKKYHFIDKKEEELGLLDYDDVGDFSEGMAKVRIGNYFDGK
                     WGYIDTTGKRVIDVKFEEAGDFYKGEASVKLNGKSMYIDKTGSNDEERKKIAEQE"
     gene            complement(1079..1615)
                     /locus_tag="PK134_05285"
     CDS             complement(1079..1615)
                     /locus_tag="PK134_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38295.1"
                     /translation="MKTTNYILVLIATLMLSACQKQEAETDNEPQVAATCDDGIMNQG
                     ETDIDCGGPCPLCQATMNAMVDADTFKAVFNTITSKYANGAFFMSGSSNLGSLTVIYS
                     GPLNIGAVSNAQATYVKSSTSVTYIASNANLTFNAINVTDSTAEGTFSFNAINFNSPT
                     DTIKITNGFFKRISIKNN"
     gene            complement(1647..2765)
                     /gene="lpxB"
                     /locus_tag="PK134_05290"
     CDS             complement(1647..2765)
                     /gene="lpxB"
                     /locus_tag="PK134_05290"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRB38296.1"
                     /translation="MRYYIIAGEASGDLHASNLALQLRKYDTGAIMRGWGGDKMQQAG
                     VEIVKHYRELAFMGFVEVIKNLRTILRNLEICKEDILAFNPDVVILVDYPGFNLRIAE
                     FLKKKNIKVFYYISPQIWAWKQSRVHKIKRDVDKMFVILPFEKDFYKKFDYDVVFTGH
                     PLLDEIEKIKHQPAKALSQSDIDKKIIALLPGSRMQEIKEMLPVMLKAVSVFKNFNPV
                     IAAAPNIPDSFYQSLAGKDIPIVHGRTYDILQQAYAALVTSGTATLETALFKVPEIVC
                     YKGNRFSYLIARQLVKVKYISLVNLILDRMLVKELIQDDLNEKTLTSEMKLLLEDEEY
                     RGNILAGYDELEAKLGMAGASEKTARKMVDLLKGTVDQ"
     gene            complement(2771..3058)
                     /locus_tag="PK134_05295"
     CDS             complement(2771..3058)
                     /locus_tag="PK134_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38297.1"
                     /translation="MFSKDKFITGFIGGIIAPVVGFYLYYIALFRQMDLSGFYNTLVS
                     NNTLAAAISLSLLANLALFFVFDKFGKLNGSRGVIAATFIYGFYIVYLKFF"
     gene            complement(3149..3313)
                     /locus_tag="PK134_05300"
     CDS             complement(3149..3313)
                     /locus_tag="PK134_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38298.1"
                     /translation="MFKKIPDTFHIGALSALALSVLAWLLISRTESYIVTSAFYSVCL
                     AACFFKNNDD"
     gene            complement(3306..4076)
                     /gene="surE"
                     /locus_tag="PK134_05305"
     CDS             complement(3306..4076)
                     /gene="surE"
                     /locus_tag="PK134_05305"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRB38299.1"
                     /translation="MSKPTILVTNDDGITASGIRALVEAMREIGHVIVVAPDSPQSGM
                     GHAITIHNPLRLDKVDVFKGVDAWQCSGTPVDCVKLAVNKILHRKPDICVSGINHGSN
                     SSINVIYSGTMSAAMEGAIEGIPSIGFSLVNYKLDADLSAAKHYAGVITKTVLGGGLH
                     HNLLLNVNIPYLQLDEMKGLKICRQAMAKWKEEFDERLDPAQQKYYWLTGKFINPDLG
                     EDTDEWALANGFVSVVPVEFDFTAHHAIPVLNKLQWNV"
BASE COUNT         1348 a          937 c          659 g         1451 t
ORIGIN      
        1 cttcttgttc agcaatcttt tttctctctt catcattact tcctgtctta tcaatataca
       61 ttgatttccc attcaatttt acacttgctt cacctttata aaaatcacct gcttcttcaa
      121 actttacatc aattactctt ttgcccgttg tgtcaatata tccccattta ccatcaaaat
      181 aatttcctat ccttaccttt gccattcctt cactaaaatc acctacatca tcatagtcaa
      241 gcagaccaag ttcttcctct ttcttatcaa taaaatgata ctttttcccc acctttacat
      301 aggcaaagcc ctcactaaaa tcacctgcgc catcatattt aaatgcaata atttcattac
      361 ctgatttatc aataaatcca tatttacctc cacttaatgt attatactta ccgcctctta
      421 ctgctccact tctagcactt gctaagcctt cactgaaatt acctacttca tcatatctta
      481 aaggaataat tacattaccc gtcttgtcaa taaatccaca tttatcatct gatttgacag
      541 ctgccaatcc ctcataaaaa tcttttgcat cttcgtattt tataggaatc acttccttcc
      601 ctgttacatc aataaatcca tatttcattt tcttatttct aacccttgca atcccttcag
      661 tgaagctcca tgcaaaatta taccttgcag gaataatttc ctttccggtt gcatctttat
      721 agccccaaag ctctttatca cttttaaaag gttttaaatc ctgagcattt ataatgtttg
      781 ttaacatcaa tgcaactaaa aaaacaacta tctttttcat attatttggt ggttacaata
      841 ctctattttt aatacatatt caaaaataat tgtagttttt actgttggga actatgattc
      901 aaaaatcttt caacaaatta taaacacctt caaactcact tacaatattg taatgagcaa
      961 ctgttctgac aaataatttt cagggggtag tccattccac agttttatgc ttcttatttc
     1021 ctcaaagttc agtataataa tttctgaagg tgtcattata ttcaactact atgtctcttc
     1081 aattattctt tatactgatt cttttaaaga aaccattggt aattttaatt gtgtcggtag
     1141 gtgaattaaa attgatggca ttaaatgaaa acgttccttc tgctgtacta tctgtaacat
     1201 tgatggcatt gaaagttaaa ttagcattgc ttgcaatata cgttaccgag gttgaacttt
     1261 ttacataagt ggcttgtgca ttgcttacag cacctatatt caatgggcct gaataaataa
     1321 ctgtcagcga ccccagatta gaagatcctg acataaaaaa tgcaccgttt gcatatttgc
     1381 ttgtgatggt attaaataca gccttaaaag tgtcggcatc aaccatagca ttcattgttg
     1441 cctgacataa cggacacggg cctccacagt caatatctgt ttcgccctga ttcatgatgc
     1501 catcgtcaca tgtggctgca acttgcggtt cgttatctgt ttctgcctcc tgcttctgac
     1561 atgcagaaag cattaaggtt gctatgagta ctaaaatata gtttgtcgtt ttcacaaaaa
     1621 taatttagca taaaagtaaa taaattttat tgatcaacag tgcccttcag caaatcaacc
     1681 atttttcgtg ctgttttttc agaagctcct gccattccca acttagcttc caattcatca
     1741 taacctgcaa ggatgttacc tctgtactct tcatcctcaa gcaacaattt catttctgat
     1801 gtcagtgttt tttcattcag atcatcctga atgagttcct ttaccaacat acggtcaaga
     1861 ataagattta ccaacgaaat gtattttacc ttaaccaact gccgtgctat gaggtatgaa
     1921 aagcggtttc ctttataaca tacaatttca ggtactttaa ataatgccgt ttctaaagtt
     1981 gctgtgccac ttgttaccaa tgcagcataa gcctgctgaa gaatatcgta ggtgcgacca
     2041 tgtacaatgg gaatatcttt accggccaat gactgataaa acgaatcggg aatattgggt
     2101 gcagctgcaa ttacaggatt aaaatttttg aaaacagaaa cagctttcaa cataacagga
     2161 agcatttctt ttatttcctg catgcgactg cccggcaaca atgcaataat ttttttatct
     2221 atatctgact gcgacaatgc cttagccggt tgatgtttga ttttttcaat ttcatctaat
     2281 agcgggtgtc cggtaaacac tacatcataa tcaaactttt tataaaaatc tttttcgaaa
     2341 ggtaatatta caaacatctt gtccacatca cgttttattt tgtgtacacg cgattgtttc
     2401 catgcccaaa tctgaggtga gatataataa aacaccttaa tgtttttttt cttcaggaac
     2461 tcagcaatgc gcaggttgaa acccggatag tcaactaaaa taactacatc aggattaaaa
     2521 gcaagaatat cttctttaca tatttcaaga ttgcgcaaaa tggtacgtag gttttttatc
     2581 acttctacaa atcccataaa tgccagctca cgatagtgtt ttacaatttc cactcctgct
     2641 tgttgcatct tatcgcctcc ccatccgcgc attatagcgc ctgtatcata tttacgcagt
     2701 tgcaatgcca gattagatgc atgcaaatcg cctgaggctt caccggcaat gatgtagtag
     2761 cgcatcgggg tcagaaaaat ttaagataaa caatgtaaaa tccataaatg aatgttgctg
     2821 caatgacacc gcgcgagcca ttcaacttac caaatttatc gaacacaaag aacagtgcta
     2881 agttagcaag caaactcaga ctgatagctg cagcaagcgt gttgtttgaa accaaggtgt
     2941 tgtaaaatcc ggaaagatcc atttggcgga acagtgcaat ataatagaga taaaaaccaa
     3001 ctaccggtgc aatgatgcca ccaatgaatc ctgttataaa cttatcttta ctgaacatga
     3061 ttacgatttt tttatcaggt aaaatacaaa cagagcaaaa ataaaaacac taaacatcca
     3121 cccacgggca aagtgttgtt tttgaaagtt aatcatcatt attcttaaaa aaacaagctg
     3181 caaggcaaac actataaaat gcagatgtta caatatatga ttctgtgcgg ctgataagca
     3241 gccacgcaag cactgacaag gccaaagcag acagtgcgcc aatatgaaat gtatccggaa
     3301 ttttcttaaa cattccactg caatttgttt aatacgggaa tggcatgatg agcagtaaaa
     3361 tcaaactcaa caggaacaac agaaacaaag ccatttgcca aagcccattc atcggtatct
     3421 tcgcccaaat cgggattgat aaactttccg gtaagccagt aatacttttg ttgtgcaggg
     3481 tctaatcttt catcaaactc ctccttccac ttggccattg cctgacggca tatttttaat
     3541 cctttcattt catcaagctg cagataagga atgtttacat tgagtaataa attatgatgc
     3601 aatcctccgc caagaacagt ttttgtaatg actcctgcat aatgtttggc tgcagacaaa
     3661 tcggcatcga gtttataatt taccaatgaa aacccaatac ttggaatgcc ttcaatagca
     3721 ccttccattg cagcagacat ggtgcccgaa taaattacat tgatagatga attgcttcca
     3781 tgattgattc cgcttacaca gatatcgggc ttacgatgca atattttatt tacggcaagc
     3841 tttacacaat ctaccggtgt gccgctacac tgccaggcat ctactccctt aaacacatct
     3901 accttatcaa gtcttaaagg attgtggatt gtgatggcat gacccatacc cgattgcgga
     3961 ctgtcgggtg ctactactat tacatgacct atttcgcgca tggcttctac caatgcacga
     4021 atgccacttg cagtaattcc atcatcgttg gtaactaaaa ttgttggttt tgacatgctg
     4081 cgaagataac agttcctacg ctttatgata tttctatctt tggtctattc actccactga
     4141 ttaaaaatca ggagggactc tgttgaggta aaaaatcagt attatttctt acaactgatg
     4201 acactcaatg tcttactact ctaattttta tatgttctgc ttttctaaaa tgtgtaaaaa
     4261 tcaattataa ctctgttgca agtctattga cttatacatt ttacctttct attattgcat
     4321 ttaaagttct aaaaaatgaa tcatcgtttt tctgctttat tgcttatgct atctattagt
     4381 attgctacat tatcc
//
LOCUS       DAORQT010000120         4377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3544_length_4377_cov_8.207311, whole genome shotgun sequence.
ACCESSION   DAORQT010000120 DAORQT010000000
VERSION     DAORQT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3544_length_4377_cov_8.207311"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PK134_05310"
     CDS             complement(<1..803)
                     /locus_tag="PK134_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HRB38300.1"
                     /translation="MDASKTLEAATSRLRKYIESHHYKGYDPYDALKSPFFKLPLLNK
                     NKLIRFGAQQLVKRSPLNLRPLLFVPKGLNPVTLGLCIQAYAQLINVNQQEKNILNQN
                     IAKLIDELEKLIPKGFHGACWGYDFDWEARYANIPAYQPTVVATGIITNALFIAWQQT
                     GNQKAFQLCKSAADFVMKDLNRTVDANGHFCFSYSPFDKQVVFNASMKGSRLLAQVYS
                     VTKDESLKKAAADSVAFVMKHQRADGAWIYSTSDAGTWIDNYHTGYVLD"
     gene            complement(808..2934)
                     /locus_tag="PK134_05315"
     CDS             complement(808..2934)
                     /locus_tag="PK134_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRB38301.1"
                     /translation="MEQLTQNLKDGKMQLLEVPFPVLSSGQVLVRNHYSLISAGTEGK
                     TVKDARLSYIGKARARKEEVKKVIDAAKTFGILNTYKMVMNKLDAPSSLGYSTAGEII
                     AVAPDVTEFRVGDLVACGGNSAVHAEVIAVPVNLCVKLNDKIALQHACFTTLGSIALQ
                     GIRQADLRLGENCVVIGLGLVGQLTVQMLKAGGIKTIGIDIDQRMVDLALHTDIDAAF
                     TRNSEDLESSINNITNGYGTDAVIITAGTDSLDPVDLAGALCRRKGKVVIVGAVPTGF
                     KRPNYFKKELDLRMSCSYGPGRYDTEYEEGGIDYPYDYVRWTENRNMAAFVDLIATGK
                     IHLSNLLTHTFDFKEAPAAYDLIMNKTEPFVGMVLKYDTNKPLQQKVNLSNPFYTKSD
                     VVAGMIGAGSFGQNFLLPALKNTPAKLSGIVTARPNNARNIADKNQFSFASGNAQDIF
                     SNGDINTVFIATRHETHAGYVMQALQAGKNVFVEKPLCLTEEELVQIQHEFTKQNCRV
                     MVGFNRRFAPMIQTIKKTFSSGNPIAINYRINAGVIPADHWVHDLKVGGGRIVGEACH
                     FIDLCMFLAGSKLTTISAVALNDAQKLNDTVCFNLQFENGSTASVSYFSNGNKNINKE
                     YLEVFGSGIVAVLDDFKTLTVFGKSEKKYTGNQDKGHQNEVKAFVDSIVQGKPAPIPF
                     EETVLSTLATFRALQSIASNGERIIL"
     gene            complement(2954..3913)
                     /locus_tag="PK134_05320"
     CDS             complement(2954..3913)
                     /locus_tag="PK134_05320"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRB38302.1"
                     /translation="MKKVLVTGGAGFIGSNLCDALLTNDLYEVHCLDVFDDFYNPAVK
                     RNNISEALKSKNFFLHEGDIRDTDFLEKLFQQPFDIIVHLAARAGVRPSIEKPLLYED
                     VNIKGTVNLLEKAKEKNIKQFVFAGSSSVYGINPNTPWSENDFVLKPISPYASTKVSC
                     ELTGHTYAHLYGIRFISLRFFTVFGPRQRPDLAIHKFFKLIRQGTPIPFFGNGETSRD
                     YTFVGDIVNGIIAAMHYDKSNYEIINLGNNRTINLNMLIAAIEKEAGKKALLEKLPDQ
                     PGDVPHTFADISKAKELLNYSPATTLEEGLIAFRKWFDQNHST"
     gene            complement(3925..>4145)
                     /locus_tag="PK134_05325"
     CDS             complement(3925..>4145)
                     /locus_tag="PK134_05325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38303.1"
                     /translation="AAISESVHDGKNGYLVEKKNIQQLAEKIIILANDKDLRNKMGAE
                     SRRIYLENLTEEKMVERMGHAFRSVIEN"
     assembly_gap    4146..4155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4156..>4377)
                     /locus_tag="PK134_05330"
     CDS             complement(<4156..>4377)
                     /locus_tag="PK134_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB38304.1"
                     /translation="EWREADVKTDFKKFLQQHPELPVINHGPVKGDKKFSILNEADIF
                     VFPTYYRNEGHPWVTVEAMAAGLPVISTD"
BASE COUNT         1264 a          867 c          778 g         1458 t
ORIGIN      
        1 caatctaaaa cataaccggt gtgatagttg tcaatccatg ttcctgcatc gctggttgag
       61 taaatccatg caccatctgc acgttgatgt ttcatgacaa aggcaacaga atctgctgct
      121 gcttttttta gggattcatc ttttgttact gaataaacct gagccaacaa acgagagcct
      181 ttcatgcttg cattaaaaac tacctgttta tcgaatggtg aataagaaaa acaaaaatga
      241 ccattggcat cgacagtacg atttaaatct ttcatcacaa agtctgctgc acttttgcaa
      301 agttgaaatg ctttttgatt tcctgtttgt tgccaggcta taaaaagtgc attggtaatt
      361 attccggtcg caacaactgt tggctgatag gcaggtatgt tggcataacg tgcttcccag
      421 tcaaaatcat atccccaaca tgcaccatga aatccttttg gaataagttt ttcaagctca
      481 tcaataagtt tagcaatatt ttggttgagt atattttttt cttgctgatt aacattaatc
      541 agttgtgcgt aagcctgaat gcaaaggcca agagtaacag gattcagccc tttcggaaca
      601 aaaagtagtg gtcgtaaatt gagtggcgat cttttaacaa gttgttgtgc gccaaagcga
      661 attaacttat ttttattcag caagggtaat ttaaaaaatg gagattttaa tgcatcgtaa
      721 gggtcgtatc ccttgtagtg atgtgactcg atatattttc gtaatcgtga tgttgccgcc
      781 tcaagggttt tggatgcatc cattgaatta aagaattatt ctttctccat tggaggcaat
      841 gctttgtaat gctcggaatg ttgccagtgt gctcaaaaca gtttcttcaa aaggtattgg
      901 tgcaggtttt ccctgaacaa tactatctac aaatgcttta acctcattct gatgaccttt
      961 atcctgattg cctgtatatt ttttttctga tttaccgaat acagtcagtg ttttaaaatc
     1021 atcaagaact gcgacaatgc cagaaccgaa tacttcaaga tattctttgt tgatgttttt
     1081 attgccattg ctgaaataac ttaccgaagc ggtactgcca ttttcaaatt gcaggttaaa
     1141 gcaaacggta tcgtttagtt tttgtgcatc attcaatgca actgcactta tagtggtaag
     1201 ttttgatccg gcaagaaaca tacataaatc aataaagtga caggcttcac caacaattcg
     1261 tccacctcca acttttaaat catgcaccca atgatcggca gggattacac ctgcattgat
     1321 tctgtaatta attgcaattg gatttccgga agagaaagtt tttttaatgg tttgaatcat
     1381 tggagcaaac cttctgttga agccaaccat taccctgcaa ttttgcttgg taaattcatg
     1441 ttgtatctga accaattctt cctctgttaa gcaaagaggt ttttcaacaa atacattttt
     1501 ccctgcctgt aatgcctgca taacataacc tgcatgtgtt tcgtgacgtg ttgcaataaa
     1561 aactgtattg atatcaccat tgctgaaaat gtcctgagca ttacctgatg caaaagaaaa
     1621 ttgatttttg tctgcaatgt ttcgtgcatt attgggacgt gctgtaacta taccactgag
     1681 ctttgcagga gtattcttta aagcaggtaa taaaaaattt tgtccgaaag aacctgcacc
     1741 aatcatacct gcaaccacat cactcttagt ataaaaaggg ttggaaagat tgactttttg
     1801 ttgcagcggc ttattcgtat catatttcaa aaccattcca acaaaaggct cggttttgtt
     1861 cataataaga tcgtatgctg caggtgcttc tttaaaatca aatgtatgag taagcaaatt
     1921 tgaaagatgg attttaccgg ttgcaattaa atcaacaaaa gcagccatgt ttctgttctc
     1981 tgtccagcgt acatagtcgt aaggatagtc aattcctcct tcttcgtatt cagtatcgta
     2041 acgccccgga ccataagagc aactcatgcg caggtctaat tcttttttaa aatagtttgg
     2101 tcgtttaaac cctgttggta ctgctcccac aataactact ttgccttttc tgcgacataa
     2161 ggctcctgcc agatcaacag gatcgagtga gtcagtgccg gcagtgatga ttactgcgtc
     2221 agtgccgtag ccgttagtaa tattgttaat tgaactttca agatcttcac tgttacgggt
     2281 aaatgcagca tcaatatcag tatgcaaagc aaggtcaacc atacgttggt caatatctat
     2341 tccgatagtt tttatgccac cggctttaag catctgaacg gtaagttgtc caaccagacc
     2401 aagcccgatg actacacaat tttcacctaa acgaaggtcg gcttgtctta tcccctgaag
     2461 tgctattgaa cctaaagttg taaagcatgc atgttgaagg gcaattttat cgtttaactt
     2521 aacacaaaga tttacaggga cagcaataac ttctgcatga actgcactgt tgccaccaca
     2581 agctaccaaa tcaccaactc tgaattcagt aacatcaggt gcaacagcaa taatttcgcc
     2641 tgctgtgctg taacctaatg atgatggtgc atctaattta ttcatcacca ttttataagt
     2701 gtttaaaatg ccgaatgttt ttgcagcatc aatcactttt tttacttctt cttttcttgc
     2761 tcttgcctta ccgatataac tcagtcgtgc atcttttacg gttttgcctt cagtacctgc
     2821 actaatcagc gaataatgat tgcgtactaa aacctgccct gatgaaagaa ccggaaacgg
     2881 cacttcaagc aactgcattt ttccatcttt cagattttgt gttaactgtt ccattttttg
     2941 cagtttagat tgtttatgtg gaatgatttt gatcgaacca ctttctgaat gctattaatc
     3001 cttcttctaa tgttgtagca ggactatagt taagtaattc ttttgcttta cttatgtcgg
     3061 caaatgtatg aggtacatcg cctggttgat caggtaattt ttcaagtaat gcttttttgc
     3121 ctgcttcttt ttctattgct gcaatcagca tattcagatt gatagttctg ttattaccaa
     3181 gatttatgat ttcgtaattg cttttatcat aatgcatggc tgcaattata ccattcacga
     3241 tgtcgcctac aaaagtatag tcgcggctag tttcaccatt gccaaagaat ggaattggtg
     3301 taccctgacg tatcagctta aaaaatttat gtatagctaa gtcaggccgt tgacgaggac
     3361 caaacactgt aaagaagcgc agagatataa acctgatacc atacaaatga gcataagtat
     3421 gcccggtaag ttcgcatgag acttttgttg atgcataagg agaaataggt ttcaatacaa
     3481 aatcattttc gctccatggt gtgtttgggt tgatgccata gacactacta ctgcctgcaa
     3541 aaacaaactg tttgatattt ttttcttttg ccttctccaa taagtttaca gttcctttga
     3601 tatttacatc ttcataaagc aaaggttttt caatactcgg tctaactccg gcacgtgcag
     3661 ccagatgaac tataatgtcg aacggttgtt gaaatagttt ttcgagaaag tcagtatcac
     3721 gaatatcacc ttcatgcaaa aaaaagtttt tactctttaa tgcttccgaa atattatttc
     3781 tttttacagc aggattgtaa aaatcatcaa acacatcaag gcaatgcact tcgtataaat
     3841 cattagttaa aagagcatca cataaattgc tgccaataaa acctgctccg cctgttacta
     3901 atactttttt caattcaata atatttaatt ctcaattaca cttctgaatg catgacccat
     3961 ccgttcaacc attttttctt ctgtcagatt ttctaaatag atgcgtctgc tttctgctcc
     4021 cattttattt ctcaaatctt tatcgtttgc caaaataatt attttttcgg ctaattgctg
     4081 aatatttttc ttttctacca gataaccatt ttttccatca tgtacgcttt cggagattgc
     4141 tgcatnnnnn nnnnnggtct gtagaaatta caggcagtcc ggcagccata gcttcaacag
     4201 taacccatgg atgaccttca ttgcgatagt atgtagggaa aacaaaaata tcggcttcgt
     4261 tcaggatgct gaacttttta tcacctttaa caggcccgtg atttatcaca ggcagttcag
     4321 gatgttgttg taaaaatttt ttaaaatctg tttttacatc tgcttcacgc cattcac
//
LOCUS       DAORQT010000121         4335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3599_length_4335_cov_11.603271, whole genome shotgun sequence.
ACCESSION   DAORQT010000121 DAORQT010000000
VERSION     DAORQT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3599_length_4335_cov_11.603271"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK134_05335"
     CDS             <1..255
                     /locus_tag="PK134_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRB38305.1"
                     /translation="VIKSPIVGGLWYRGYTGKKFLSSDSFIALIGLQKDMFKFGYSYD
                     VTVSKLNNNVTAGSHEISLGLQFECRPKKKRFKAISCPTF"
     gene            297..1745
                     /locus_tag="PK134_05340"
     CDS             297..1745
                     /locus_tag="PK134_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB38306.1"
                     /translation="MNLRKNAKTLAFVALVVMATNACKQKEKSSTTGWKYNDEKNGGF
                     EVTPYTEQETGPGLILVEGGTFAMGRTEQEIPYDWDNIPRRVTVSSFYMDETEIANVH
                     YLEYLYWLDRVYGTDYPEVYKKALPDTLVWRSKLGYNEPMVELYLRHPAYQRYPVVGV
                     NWLQANDFASWRTDRVNEQILVREGILRVNPAQVNEDNFNTDAYLAGQYEGLVKSKLQ
                     DLNPNRDERNVRMEDGILLPRYELPTEAEWEYAALGLIGNTVYERVTDRKIYPWNGHV
                     VRNSSEKYKGEMLANFKRGRGDMMGTAGALNDNADRPAPVFSYLPNDYGLYNMAGNVS
                     EWVKDVYRPLSPEDMDDFNPYRGNVFVTPLLDEEGQYAEKDSLGRMVWREVDEKENEA
                     RRNYTKADYINDLDGDEPDNIEYKMGGTTMIDDNAHVIKGGSWKDRAYWMSPGTRRFL
                     DQQQSTDWIGFRCSMVRVGSPVGFNQKASKKG"
     gene            1820..3121
                     /gene="murF"
                     /locus_tag="PK134_05345"
     CDS             1820..3121
                     /gene="murF"
                     /locus_tag="PK134_05345"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRB38307.1"
                     /translation="MIAHLHSLFLNSTGVCTDTRKLLKGNIYFALKGPSFDGNKFALQ
                     AIQQGAIAAIVDDSSLKGENIYLVENVLTTLQQLATYHRQVLKIPVIGLTGSNGKTTT
                     KELISRVLGTSYNVLFTEGNLNNHIGVPLTLLRLKSEHQIAVIEMGANHQGEIRELSM
                     IALPNFGLITNIGKAHLEGFGGIEGVIKGKTELYRHLMENDGVIFCRKNNELLTNNLN
                     HYQKVIFYSTQVDNCICGEISTPASVVKFNWFENQNRIDEIITHLTGDYNLENLVAAV
                     CIGRYFNITPDKIKTAIEGYIPENQRSQIEISGSNTIIIDAYNANPSSMEAALKNLSE
                     NYDGNKVIILGEMLELGQDANTEHQHIVDLLSSMEKDLLILVGENFLHTTLNDHALRF
                     KTSEDACNYLHNHAINESTILVKGSRGSKMEKVLDGIRNHN"
     gene            3194..3781
                     /locus_tag="PK134_05350"
     CDS             3194..3781
                     /locus_tag="PK134_05350"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRB38308.1"
                     /translation="MKNTNTEIFNDDIKSCLEILRQGGLIVYPTDTIWGIGCDATNDA
                     AVEKIFKLKNRSESKSLITLVSDEAMLNRCVQNVPAVAWDIIEYSEKPVTIIYEKISR
                     ISEKAIAEDGTAAIRLVKDSFCKMLIHKFGKPIISTSANMSDRPSPAVFNDIEHSILS
                     GVDYVVKYRQQENTKHTASSIIRILNNGEVKVIRK"
     gene            3753..>4335
                     /locus_tag="PK134_05355"
     CDS             3753..>4335
                     /locus_tag="PK134_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HRB38309.1"
                     /translation="MAKLKLSENNLQTEMLEHSLFRQIASLCEKHNLKAFVIGGFVRD
                     SFLKRPSKDIDIVVNGDGIAVAKELAAVLEGEFAFFKNFGTAMVKSEGFEIEFVGARK
                     ESYSQHSRKPKVSPGTLEDDQLRRDFTINTLAFSLNSDDYGKLIDPFNGKQDLNDKII
                     RTPADPVTTYSDDPLRMMRAIRFATQLNFSIEEK"
BASE COUNT         1482 a          727 c          872 g         1254 t
ORIGIN      
        1 gttataaagt cgccaattgt tggtggtttg tggtacaggg gctatacagg taaaaaattc
       61 ctgtcaagcg acagcttcat tgccctcatc gggttacaaa aagacatgtt taagttcggt
      121 tattcctatg atgttaccgt ttctaaacta aataataatg ttactgccgg atctcatgaa
      181 atttctttag gtttgcagtt tgaatgccgt ccaaagaaaa aacggttcaa ggcaataagt
      241 tgccccacat tttagtttaa tgacataaac catataaagt ttccaaatac gtaaaaatga
      301 atctccgtaa aaatgcaaaa acccttgcct tcgttgcttt agtagtgatg gcaacaaatg
      361 cttgcaagca aaaagagaaa tcttcaacca caggatggaa gtacaatgac gaaaagaatg
      421 gtggatttga agttactcca tataccgaac aggaaacagg tccgggatta atccttgtag
      481 aaggtggaac atttgcaatg ggccgtacag agcaagaaat tccttatgac tgggataaca
      541 ttcctcgcag agttactgtt tcatcttttt acatggacga aacagaaatt gccaatgtac
      601 attatttaga atatctctat tggttagacc gtgtttatgg aactgactat cctgaagtgt
      661 ataaaaaagc gcttcctgac acattagttt ggagaagtaa attaggttat aacgaaccaa
      721 tggttgagtt atatttacgt caccctgcct atcagcgcta tcctgttgtt ggtgtaaatt
      781 ggttacaagc caatgatttt gcaagttggc gtaccgatcg tgtaaatgag cagatattag
      841 tgcgcgaagg aattttacgt gttaaccctg cacaggtaaa cgaagataac tttaatactg
      901 atgcatatct tgccggacag tacgaaggtt tggtgaaaag caaacttcag gatttaaacc
      961 ccaatcgtga tgaacgtaat gtgcgtatgg aagatggaat tttattacca cgctacgaac
     1021 ttccaacaga agcagaatgg gaatatgcag ccttaggctt aattggcaat acggtttatg
     1081 agcgtgttac tgacagaaaa atataccctt ggaatggtca cgtagtaaga aattcttcag
     1141 aaaaatataa aggtgaaatg cttgccaact tcaagagagg tcgtggtgat atgatgggaa
     1201 cagcaggtgc attaaatgac aatgcagacc gtccggctcc agtattttca tatttaccaa
     1261 atgattatgg tttatataat atggcaggta acgtttcgga atgggtaaaa gatgtttatc
     1321 gccccttatc acctgaagac atggatgact ttaatcctta cagaggaaac gtatttgtaa
     1381 caccattact tgacgaagaa ggacaatatg cagaaaagga tagtcttggt cgcatggtat
     1441 ggcgcgaggt agatgaaaaa gaaaatgaag ctcgcagaaa ttatactaaa gccgattaca
     1501 taaacgatct tgatggtgat gaacctgata acatcgaata taaaatgggt ggtactacga
     1561 tgattgatga taatgcacac gttattaaag gtggttcctg gaaagaccgt gcatattgga
     1621 tgtcacccgg tacaagaaga tttttagacc aacaacaatc aactgactgg attggattcc
     1681 gttgttcaat ggtacgtgta ggtagccctg ttggttttaa tcaaaaagcg tctaagaaag
     1741 gataatatcc ttcaacattt aaataaaata cataagcccc tttttttggg gctttctttt
     1801 tgaaagttat tgaatataaa tgatagcaca tttacatagt ttattcctga actcaaccgg
     1861 agtttgtaca gatacaagga aactgctcaa aggaaatatt tattttgctt taaagggacc
     1921 ttcttttgat ggaaataaat tcgcactaca agcaatacaa caaggtgcca tagcagcaat
     1981 agtggatgat tcctcactaa agggtgaaaa catttattta gtagagaatg ttctaacgac
     2041 tttgcaacag ttagctacct atcacaggca agttttaaaa ataccggtta ttggattaac
     2101 aggtagcaat ggaaaaacta ctacaaaaga attaatcagc agggtactcg gcacgtcata
     2161 taacgtttta tttactgagg gaaatctgaa taatcacatc ggtgttccac ttaccttgtt
     2221 aagattaaaa agcgaacatc agattgcagt tattgaaatg ggagccaatc atcagggaga
     2281 aattcgtgaa ctgtcaatga ttgctctgcc caacttcggg ctcattacaa atattggcaa
     2341 agcacatctc gaaggttttg gaggtattga aggtgtaatt aaaggaaaaa cagaattgta
     2401 cagacacctg atggaaaatg atggagtaat tttctgccgt aaaaacaatg aattgctaac
     2461 aaataatctc aatcattatc aaaaggtaat tttctacagt acgcaagtgg acaattgtat
     2521 ctgtggtgaa atttctactc ctgcatcagt tgttaaattt aattggttcg aaaatcaaaa
     2581 taggattgat gaaataataa cgcaccttac cggtgactat aatcttgaaa atcttgtagc
     2641 ggcagtttgc attggtagat attttaatat tacacctgat aaaattaaga cagcaattga
     2701 aggttacatc cccgaaaatc agcgttcgca aatagaaatc tcaggaagta atacaattat
     2761 tattgatgca tacaatgcca atccttcaag tatggaagct gcattaaaaa atctttctga
     2821 aaattatgat ggcaataaag ttatcattct tggagaaatg cttgaacttg ggcaggatgc
     2881 aaatacagag caccaacaca ttgttgactt attgagtagt atggaaaaag acttacttat
     2941 tttagtaggt gaaaattttc tgcatacaac actcaatgat catgcattgc gctttaaaac
     3001 ctccgaagat gcttgcaact atttacataa tcatgctatt aatgaaagca ctattttagt
     3061 taaaggcagt cgcggttcta aaatggaaaa agtgcttgat ggcatccgca atcacaactg
     3121 atttttttaa aaaatcatta gctcaagaat taaaaagtaa aaattcgttt caatctgttt
     3181 tctttgcatt gtgatgaaaa atacaaatac cgaaattttt aatgatgaca ttaaatcttg
     3241 tcttgaaatt ttaagacaag gaggacttat tgtttatcct actgacacaa tttggggaat
     3301 tggatgtgat gctacaaacg atgctgcagt agaaaaaatt tttaaactta aaaaccggag
     3361 cgagtcaaaa agtttaatca cattagtgag cgatgaggca atgctgaatc gttgtgtgca
     3421 aaatgttccg gctgttgcat gggacatcat tgaatattcc gaaaagcctg taactataat
     3481 ttatgaaaag atcagtcgta tctctgaaaa agcaattgca gaagacggca ccgcagcaat
     3541 cagattagtg aaagattcat tttgcaaaat gctgattcat aaatttggga aaccaatcat
     3601 atctacttct gcaaacatga gcgacaggcc ctcccctgct gtttttaatg acattgaaca
     3661 ttccatactt tcaggagttg actatgttgt aaagtacaga caacaagaaa acacaaaaca
     3721 tacagcatca tccatcatca ggattttgaa caatggcgaa gttaaagtta tcagaaaata
     3781 atctgcagac agaaatgctg gaacatagtt tgttccggca gattgcttca ctttgcgaaa
     3841 agcataattt aaaagcattt gtcattggag gatttgtgcg tgatagtttt ttaaaaagac
     3901 cttcaaaaga tattgatatt gtagttaacg gtgacggcat tgccgtagct aaagaacttg
     3961 ctgcagttct tgaaggagaa tttgcattct ttaaaaactt tggtactgca atggtaaaat
     4021 ccgaaggatt tgaaattgaa tttgtaggtg caagaaaaga gtcgtatagt caacattcgc
     4081 gaaagccgaa ggtttcacct ggcacattag aagacgatca gcttagacgc gattttacta
     4141 tcaacacact tgcttttagt cttaacagtg atgattatgg aaagctgatt gacccattta
     4201 acggcaagca ggatctgaac gataaaatta ttcgcacacc tgccgaccct gtaacaactt
     4261 acagtgatga tccactgcgc atgatgcgtg caatacgctt tgcaacacaa cttaatttca
     4321 gcattgaaga aaaat
//
LOCUS       DAORQT010000122         4324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3612_length_4324_cov_4.850316, whole genome shotgun sequence.
ACCESSION   DAORQT010000122 DAORQT010000000
VERSION     DAORQT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_4324_cov_4.850316"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1333)
                     /gene="kynU"
                     /locus_tag="PK134_05360"
     CDS             complement(47..1333)
                     /gene="kynU"
                     /locus_tag="PK134_05360"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRB38310.1"
                     /translation="MHFINSSDFANQLDEQDPLKSFRQKFLFPQHQNKDVIYFCGNSL
                     GLQPKQTRDYVLQELNDWKDFGVEGHFQAKNPWYPYHEFLRDNMALIAGALPSETVVM
                     NSLTANIHFMLVSFYKPTTKRFKIICEGSAFPSDQYALQTQVKFHGFNPDNAIIELHP
                     RTGEHTIRHEDILETISKHKDELVLVFLGNPNYYTGQVFDMAGITQAGHAAGAIVGFD
                     LAHGIGNLELHLHDWNVDFAVWCSYKYLNAGPGGVAGAYVHQQHCNNTDLIRFAGWWG
                     NDPQTRFTMPHDFIPVKSADSWQLSNAPVLPMAALRASLEIFAEAGVQNLIKKRKLMN
                     DFLRFIIGDIISKTNSEKNIIMITPENENERAAQVSMLCKHNGKKIHGYLTEQGIIAD
                     WREPEVLRIAPVPLYNSFNDIYQFGKCLHEALLLNR"
     gene            complement(1408..1971)
                     /gene="gmk"
                     /locus_tag="PK134_05365"
     CDS             complement(1408..1971)
                     /gene="gmk"
                     /locus_tag="PK134_05365"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRB38311.1"
                     /translation="MTKHFLFCGPSGSGKTSIVQHLLTKFDFMQFSISATTRPKRANE
                     TDGKDYYFMSIENFRKHIDRNDFVEWEQVYEGRYYGTLKSEVERIEALNCSVVFDVDV
                     EGGLKIKKHFGKNLLAVFIRPPSVEDLRQRLTARNTETAESLEIRLNKSIKELSYQDR
                     FDNVIINGELNQSCKEAEALVSAFLQN"
     gene            complement(1968..2840)
                     /locus_tag="PK134_05370"
     CDS             complement(1968..2840)
                     /locus_tag="PK134_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRB38312.1"
                     /translation="MIRSMTGYGKASGDYKSKTISVEIKSLNSKFFDLNMRLPHPYKD
                     KEMDLRSLLLKEAERGKMDVMITMESDLAQQENLLNREIIKSLLTDLKAVKKEFALPD
                     DNLLQIVMQMPQVVNTDSDDADENEWKYIFSIINQAIEKFNLFRQREGHNLQQDISQR
                     MNLILQLLEQITPFEEGRINAVRSKLISSLKSLGGNIETDKNRFEQELIYYLEKLDIT
                     EEKVRLAEHCRYFLSMLNDENNNGKKLGFIMQEIGREINTIGSKANDADIQRKVVEMK
                     DEAEKIKEQLSNIL"
     gene            complement(2887..3795)
                     /locus_tag="PK134_05375"
     CDS             complement(2887..3795)
                     /locus_tag="PK134_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB38313.1"
                     /translation="MKATTRAHLSVLFVNLLYAANFSIAKQVMPEFIKPFGFIVIRVI
                     PATVLFFLAAFLIPSEKIKKKDRNTILLCALFGVAINQLLFFKGLSLTSPINGALIMT
                     TNPVLVLLMAAIILKEPVGWIKYIGITLGIAGAMLLILLGKNFNLQGGNTEGDLYIFL
                     NSVSFAIFIILVKPLMLHYHPITVMKWTFFFGALMVLPFGFNEVTAVAWSSLTFNLWL
                     CILFVIVAVTFVAYLMNVFALRHLSPSVVSIYIYFQPVFATLISMALGEASPGWLHLA
                     SAVLIFTGVYLVSKQKIAGINNTENS"
     gene            complement(3799..4176)
                     /locus_tag="PK134_05380"
     CDS             complement(3799..4176)
                     /locus_tag="PK134_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRB38314.1"
                     /translation="MKKIYHLANCTTCQRILTELKPGKDFILQDIKSEPITEQQLDAM
                     QKLAGSYEALFSRVAMKYRSMGLNQIKLSEKDYKKYILEEYTFLKRPVIIIDKEIFIG
                     NAPKTVAAAKAAMHKNKNQTKNH"
BASE COUNT         1325 a          813 c          784 g         1402 t
ORIGIN      
        1 gaaccaacaa gaccggcacc tatgattgaa atttttttca ttgttgttat ctgtttagaa
       61 gcaaagcttc gtgcaggcat ttgccaaatt gataaatgtc gttaaatgaa ttgtaaagcg
      121 gaacaggagc aatgcgtaac acttcaggct cgcgccagtc tgcaataatg ccttgctctg
      181 tcaggtagcc atgaattttt ttgccgttat gtttacatag catactcacc tgtgcagcac
      241 gttcgttttc attttcaggt gttatcatga tgatattttt ttctgaattt gtcttgctga
      301 taatgtcacc aattataaat cgcagaaagt cattcatcag cttgcgtttt tttatcagat
      361 tttgtactcc ggcttcggca aaaatttcaa gtgaggcacg caatgctgcc attggaagca
      421 caggggcatt actcaactgc caagaatcgg cacttttaac aggaataaaa tcatgcggca
      481 tagtgaaacg tgtttgtgga tcgttgcccc accaacctgc aaatcgaatc agatcggtgt
      541 tgttacaatg ttgttgatga acataagcac ctgcaacacc accgggtccg gcattgaggt
      601 acttgtaact acaccatact gcaaagtcaa cattccagtc gtgaagatgc aattcaagat
      661 tacctatgcc atgagctaaa tcgaaaccta caattgctcc tgcagcatgt cctgcctgag
      721 taattcctgc catatcaaaa acctgccccg tatagtaatt tgggttacct aaaaaaacca
      781 agaccagctc atctttatgc tttgaaattg tttcaagaat atcttcatgc ctgatggtat
      841 gttcacctgt gcgcggatgc aactcaatga tggcattatc aggattaaag ccgtgaaatt
      901 ttacttgtgt ttgtaatgca tactgatcag atggaaatgc cgacccttca cagattattt
      961 taaaacgttt tgttgttggt ttgtaaaacg acaccaacat gaaatgaata ttggcagtga
     1021 gtgaattcat aaccacggtt tctgacggta gtgcacctgc tataagtgcc atattatctc
     1081 tcagaaattc gtgatatgga taccatgggt tttttgcctg aaaatgtcct tcaacaccga
     1141 aatctttcca gtcgttcaac tcctgcaaaa catagtcgcg cgtttgttta ggttgaagtc
     1201 caagagagtt gccgcagaaa tagatgacat ctttattctg atgttgcgga aaaagaaatt
     1261 tctgcctgaa ggattttaaa gggtcttgct catcaagttg attggcaaaa tcggatgaat
     1321 tgataaagtg catgtaagta atttgaatac aaataaacaa aaaacattat cgaaaggata
     1381 taaaaacctg ctggtaaaaa gtaaaattca attttgtaaa aaagcgctga ctaatgcctc
     1441 ggcttctttg catgattgat tcaattctcc attgataata acattatcga aacggtcttg
     1501 gtatgacaat tctttaatgg atttattaag gcgaatttca agagattctg ctgtttctgt
     1561 attgcgtgcc gttagtcttt gacgaaggtc ttcaaccgat ggcggccgta tgaaaacagc
     1621 aagtaaattt ttgccaaaat gttttttaat ctttaatcct ccttcaacat caacatcaaa
     1681 aactactgaa cagttaagcg cttctatacg ttcaacctct gattttaatg tgccatagta
     1741 acgaccttca tacacttgct cccattcaac aaagtcattc ctgtcaatat gttttctgaa
     1801 attttctata gacataaaat aataatcttt tccgtcagtt tcattagcac gctttgggcg
     1861 tgtagttgct gaaatactga attgcataaa atcaaacttc gttaacagat gttgtacgat
     1921 gcttgttttg ccactaccac tcgggccaca gaaaagaaaa tgttttgtca taaaatatta
     1981 gagagctgtt ctttaatttt ttcagcttcg tctttcatct caacaacttt gcgttgtatg
     2041 tctgcatcat tagctttgct gccaatagta tttatttcac gaccaatttc ctgcataata
     2101 aagcctaact ttttgccatt attattttca tcattcaaca tagataaaaa atagcggcaa
     2161 tgctctgcaa gtctcacttt ttcttctgta atgtctaatt tttcgagata ataaatcaac
     2221 tcttgttcaa aacggttttt gtctgtttct atatttccgc caagagactt taatgagctg
     2281 attagtttac tgcgcactgc atttattcgc ccttcctcaa aaggagttat ttgttcaagt
     2341 aattgcaaaa taagattcat gcgttgcgaa atatcttgtt gcagattatg tccttcgcgc
     2401 tgacggaaaa gattaaattt ttctattgcc tgatttatta ttgaaaaaat atatttccat
     2461 tcattttcat cagcgtcatc agaatcggta tttactacct gaggcatttg cattacaatt
     2521 tgcaaaaggt tgtcatcggg caatgcaaat tcttttttaa ctgcttttaa atcggtcaac
     2581 agtgatttta ttatttcacg gttgagtaaa ttttcttgtt gcgcaagatc ggactccata
     2641 gtaatcatca catccatctt tcctctttca gcttctttta ataacaagct gcgcaggtcc
     2701 atttctttat ctttgtaagg atgtggcagg cgcatgttaa gatcaaaaaa tttactgttg
     2761 agcgatttta tctcaacaga gatagtctta ctcttatagt caccggaggc tttgccatag
     2821 ccggtcatgg aacgaatcat acttcacaaa attttggcta aagtaatcag aatgataaca
     2881 gcaacatcag ctattctcag tattatttat acctgcaatt ttttgcttac ttactaaata
     2941 gacacctgta aaaattaaaa cagcagaggc taagtgcagc caacccggac tggcttcgcc
     3001 aagagccatt gaaattagtg tagcaaaaac aggctgaaaa taaatgtaga tactcactac
     3061 agaagggctg agatgacgca atgcaaacac attcatcaga taagcaacaa aagttacggc
     3121 tacaataaca aagagtatgc aaagccataa gttaaaagtc aggctgctcc atgctaccgc
     3181 tgttacttca ttaaatccaa aaggcaaaac cattaaggca ccaaaaaaga atgtccattt
     3241 catgacagtt ataggatggt agtgcaacat gagtggtttt accaaaataa taaatatggc
     3301 aaatgaaaca gagtttaaga aaatataaag atcaccttct gtgtttcctc cctgaagatt
     3361 gaagttctta cccaaaagta tgagtaacat agcaccggca ataccaaggg taattccaat
     3421 atactttatc catcccacag gttctttaag tattatggca gccatcagta aaaccaaaac
     3481 agggtttgtt gtcattatta atgcaccgtt tatgggtgat gtgagtgaaa gacctttaaa
     3541 aaacaaaagt tgattgatgg caacgccaaa gagtgcacag agaagaatag tgtttctgtc
     3601 tttttttttg attttttctg atggtattaa aaatgctgca agaaaaaaca aaactgtagc
     3661 cggaattact ctgatgacaa taaatccaaa aggtttaata aattcaggca tcacctgttt
     3721 ggcaatgctg aagtttgccg cataaagcag gttaacaaaa agaacagaaa ggtgagcgcg
     3781 tgttgtggct ttcaagcctt agtgattttt tgtttgattt ttatttttat gcattgcagc
     3841 tttagctgca gcaactgttt ttggtgcgtt tccaataaaa atttctttgt ctatgatgat
     3901 aaccggtcgt ttcagaaaag tatattcttc aagaatgtat tttttgtagt ctttctctga
     3961 aagttttatc tgattgagtc ccattgaacg gtatttcatg gctactctgc taaataacgc
     4021 ctcatagctg cctgcaagtt tttgcattgc atcaagctgc tgttccgtga tgggttcact
     4081 ttttatatcc tgtaaaataa aatcttttcc gggtttcagc tctgttaata ttctctgaca
     4141 ggtggtgcag ttggcaagat gatatatctt tttcattcaa ctttcttgtg taaaatagtt
     4201 ttacaaagaa catgaaaaaa catcagattt tggtattcct tatcttatgt tgtccatcac
     4261 cggcaatttg ttttatcata ttgcggaaaa taaaaaaatg aaaaggcaac atggaatacc
     4321 agta
//
LOCUS       DAORQT010000123         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3615_length_4322_cov_9.144832, whole genome shotgun sequence.
ACCESSION   DAORQT010000123 DAORQT010000000
VERSION     DAORQT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3615_length_4322_cov_9.144832"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..669)
                     /locus_tag="PK134_05385"
     CDS             complement(160..669)
                     /locus_tag="PK134_05385"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HRB38315.1"
                     /translation="MKQANLFFVPITILLLSAIFSCNTSKKVSENNSADSLLISMSRF
                     PCFGRCPYYDASLYKSGYAVINRKGFMDSLGIYATQIDKQELNNFVEDAKKAGLIAMP
                     DSFYNPGIADYPATIVTVNLNGKRKRIFDGVPESPSSLKDFEEKVHLFFTAPNRQWKL
                     IQKPAQQQD"
     gene            767..2068
                     /locus_tag="PK134_05390"
     CDS             767..2068
                     /locus_tag="PK134_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRB38316.1"
                     /translation="MKSIKIMGVLLLSIATVALLTSMKKGSGRYKVKTKGEKGCVFRS
                     VFEAEGIDSNFIYSTPVKVKESMTSALEWISKAQLKDGGWGAGSHARQSVFDPHAVSS
                     DPATTAMVAMALLRTGSTPTTGKYATNLSLATEYLLKQIESADAQTLNITNQTATQPQ
                     VKLGQNIDVVLATQYLTNLMDYISNNQTLKTRVSKSLDKCVVKIQHAQSENGSIKGAG
                     WAGVLQSSFATNALESAKAAGANVDEEKLERYKNYQKGNYDAKTNSVATGDGAGVMLY
                     SISGTARSTAREASIAKDKISRAKKEGKLAPTSTVTSESLQAAGMSEADAYKYAAAYE
                     INQAASKQAQRNDVMNGFGSNGGEEFLSYLQTGEGLIISKDKEWHKWYDNVSGRLLSI
                     QNNDGSWNGHHCITSPVFCTATSLLILSVNNDIEKLVSLNK"
     gene            complement(2111..3178)
                     /locus_tag="PK134_05395"
     CDS             complement(2111..3178)
                     /locus_tag="PK134_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006071841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB38317.1"
                     /translation="MTEKRELSAFEAKNEAQKLAFAPVVFQVVVAMQRLGILPLICKN
                     RKGITVKEISEKTNVSIYGVKVLLEMAANADIVKYLDDSTVTVTMLGYFLNSDEMTRV
                     NINFTQDVCYDGFKFLTESIQNGKPEGLKTLGNWPTIYPALSSLPEHIKKSWFEFDHF
                     YSDEAFRDALNIVFKENPSTIFDIGGNTGKWAFASCEFNPDVHVKILDLPQQIAVAKA
                     NAEKRNLTERISYFEINLLDKSQKIPKGADVVWMSQFLDCFGEDEIVSILENVRQAAS
                     PHTTVFILEPFIDNQKFEAASYCLTATSLYFTALANGNSKMYSVKAMTGLVEKSGLEV
                     VETFPLIGESFHTILKCKVKV"
     gene            complement(3204..3929)
                     /locus_tag="PK134_05400"
     CDS             complement(3204..3929)
                     /locus_tag="PK134_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRB38318.1"
                     /translation="MNKIFRFHLSRHILWFCLLSFFLSSCRGIFLSKDKAYSKAIATK
                     PYDAIIVPGFPYFEGQPWPPVVQMRMIWAKFLYQNGYTKNIIFSGNAVYSPFVEGKTM
                     KAFAIAMGIPEEHIYVEDKAEHSVENVYYSYRLAKKLGFKKIALATDPVQTRSMVSFI
                     HRFSLPISYLPIMYKTLRLLPHSEPDIDGKLQRVENFISLPEKETFKQRFAGTLGKNI
                     KWHAEDLKKKRLRKKYSHKIVQE"
     gene            complement(3934..>4322)
                     /locus_tag="PK134_05405"
     CDS             complement(3934..>4322)
                     /locus_tag="PK134_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38319.1"
                     /translation="FKLTNDKYETNVDVVQDINSKINVHERVMNGSYSIGEDDVKKAV
                     SHYKSKEKDAIGLVYVVESFNKTLEKAVIWVTFVDLSNNKMLYTEKVEEKAGGFGLKN
                     YWIAPATKLKKEIESKKYKIWKNKFK"
BASE COUNT         1387 a          792 c          790 g         1353 t
ORIGIN      
        1 gtaacgtaaa gtggctgcaa ttcaaagaga tttgcaaagg atgattgcct aaaaactatg
       61 ctttaaatga ttggtgcatg aatttagaac cctcatcagc aaactgttgt caacagaaat
      121 gccaaaaagt atttggcgac ataatgacat aaaagctttc taatcctgct gttgtgcagg
      181 tttttgtatc agtttccact gacgatttgg tgctgtaaaa aacagatgaa ccttttcttc
      241 aaaatctttt aaagaagaag gagattcggg cacaccatca aaaatgcgtt ttcttttacc
      301 attcaaattt acggtgacga tagtagcagg gtaatctgca attccgggat tataaaaact
      361 atcgggcata gcaataagtc ctgccttctt tgcatcttca acaaaattat tcaactcctg
      421 tttatcaatc tgtgtagcat aaataccgag gctatccata aatccttttc tgttgatgac
      481 tgcataaccg ctcttatata atgatgcatc ataatatggg catcggccaa agcatggaaa
      541 acgactcatg ctgatgagta aactatctgc tgaattattt tcagaaactt tttttgaagt
      601 gttgcatgaa aagatagctg ataaaagtag tattgttata ggaacaaaaa ataaattggc
      661 ctgtttcatt ctgcaaatgt gaaaataaca tttgcctttg tcaaataaat gtgtgaaatt
      721 ttgtgcgtag ttacatcctt agtaaaatca ataaaaattt aaggaaatga aaagtataaa
      781 aattatggga gtgctattac tctctatcgc aacagtggca ttacttacat caatgaaaaa
      841 aggtagtggc cgttacaaag taaaaaccaa aggagaaaaa ggatgtgttt tccgttctgt
      901 ttttgaagca gaaggtattg actcaaattt tatttattca acaccggtaa aagttaaaga
      961 atctatgaca tctgcattgg agtggattag caaagcacag ttaaaagatg gtggctgggg
     1021 agcaggctca catgccagac agtcggtttt tgacccacat gctgtaagtt ctgaccctgc
     1081 cacaacagct atggttgcca tggccttgct tcgcacagga agcacaccta caacaggaaa
     1141 atatgctacc aatctttctt tagcaacaga atatttattg aagcaaatag agtctgcaga
     1201 tgcacaaact ttaaatatta ccaatcaaac tgcaacacaa ccacaggtta aacttgggca
     1261 aaatatagat gtagttcttg caacacaata cctgaccaat ctgatggatt acatatctaa
     1321 caatcagaca ttgaaaacga gagtaagtaa gagtcttgat aaatgtgttg taaaaataca
     1381 acatgcccag agcgaaaatg gaagtataaa aggtgcagga tgggcaggtg ttttacaatc
     1441 atcgtttgca actaatgcat tagaaagtgc aaaagccgca ggagcaaatg ttgacgaaga
     1501 aaaacttgag cgatataaaa attatcagaa aggtaactat gatgcaaaga caaattccgt
     1561 agctacaggt gatggtgccg gagtaatgtt atacagtatt tcgggcactg ctcgcagcac
     1621 agcacgcgag gcaagtattg caaaagataa aatatcgaga gcaaaaaaag aaggcaagct
     1681 agcgcctact tcaactgtaa caagcgaaag tctgcaggct gccggcatga gtgaggctga
     1741 tgcttacaaa tatgctgctg cttacgaaat taatcaggct gcaagtaagc aggcacagcg
     1801 taatgatgtg atgaatggat ttggcagcaa tggtggtgaa gagtttcttt catatctgca
     1861 aaccggtgaa ggtttaataa tcagtaagga taaagagtgg cataaatggt atgataatgt
     1921 tagcggaaga ttgttgagca tccagaataa tgatggcagt tggaatggac atcactgtat
     1981 tacaagtccg gttttttgta cagcaacttc attacttatt ctatctgtta ataatgatat
     2041 tgaaaaattg gtttcgctga ataaataaaa gaattacggg agtgctgcat ggcagcactc
     2101 ccgtaatttt ttaaactttt actttacact tgagtatagt atgaaaactt tcgccaatta
     2161 atggaaatgt ctcaaccact tcaagccctg acttttccac caaacctgtc attgctttta
     2221 cactgtacat tttactgttg ccgtttgcaa gcgctgtaaa atacagtgat gttgcagtaa
     2281 ggcaataaga agcggcttca aacttttgat tatcaatgaa tggttcgaga ataaaaacag
     2341 tagtgtgagg tgatgctgcc tgtctgacat tctcaagaat agagacaatt tcatcttcac
     2401 caaaacaatc taaaaactga ctcatccaaa caacatcggc acctttagga attttctgac
     2461 ttttatcaag caggttgatt tcaaaatatg aaatgcgctc tgttagattt cttttctctg
     2521 cattggcttt tgccactgca atttgttgtg gcaggtcaag aatttttaca tgcacatcag
     2581 gattgaattc acaggaagca aatgcccatt tgccggtatt gccaccaata tcaaaaatag
     2641 ttgatggatt ttctttaaac acaatgttga gcgcatcacg gaatgcttca tcgctataaa
     2701 aatgatcaaa ctcaaaccac gattttttaa tatgttctgg caatgatgat aaagcaggat
     2761 aaatagttgg ccagttgccg agcgttttta atccttcggg tttaccattt tgaatagact
     2821 ctgtcagaaa tttgaatcca tcataacaaa catcctgtgt aaagtttatg ttaacacgag
     2881 tcatttcatc agaattcaaa aaatagccga gcatagtaac tgttacagta ctgtcgtcta
     2941 aatattttac aatatctgca ttggctgcca tttcgagcaa aacttttact ccataaatac
     3001 ttacgttagt cttttcactt atctctttta cagtaattcc ttttctattt ttacaaatca
     3061 atggtaaaat gcctaagcgt tgcatggcaa ccactacctg aaaaactacc ggagcaaacg
     3121 ctaatttttg cgcctcgttt tttgcttcaa atgctgataa ttctcttttt tctgtcattg
     3181 tattttctgc tgaacccatc tttctattcc tgaactattt tgtgtgaata ttttttacgc
     3241 aatctttttt tcttcaggtc ttcggcatgc cattttatgt ttttacctaa agttcctgca
     3301 aagcgttgtt taaacgtttc tttctcaggc aaagaaataa aattttctac tctctgcaat
     3361 tttccatcaa tatcaggctc actatgcggc agtaaacgaa gtgttttgta cataatgggc
     3421 agataggaga ttggcagact aaagcgatgg ataaaactta ccattgaacg ggtttgaacg
     3481 gggtctgttg ccaaggcaat ttttttaaat cctaacttct ttgccaaacg atatgaataa
     3541 tacacatttt ctacgctgtg ctctgctttg tcttctacat agatatgttc ttcgggtatg
     3601 cccatggcta tagcaaatgc tttcattgtc tttccttcga caaagggtga gtaaacagca
     3661 ttgcccgaaa aaataatatt tttagtgtag ccattctgat atagaaattt tgcccaaatc
     3721 attctcattt gtactacagg tggccagggc tgaccttcaa aatatggaaa cccgggaaca
     3781 ataattgcat cataaggttt tgttgcaatt gctttgctgt atgctttatc cttagataaa
     3841 aaaattcctc tgcaagaaga aagaaaaaaa gaaagcaggc aaaaccatag aatatgcctg
     3901 cttaaatgaa agcggaaaat tttattcacc tatttacttg aatttattct tccagatttt
     3961 gtatttctta gactcaattt cttttttaag cttggttgca ggcgcaatcc agtaattctt
     4021 caatccaaaa ccacctgctt tctcttctac cttttctgtg taaagcattt tgttatttga
     4081 caggtcaaca aatgttaccc agataacagc tttctctaaa gtcttattga aactttcaac
     4141 tacataaacc aaaccaattg catctttctc cttcgattta taatgtgaaa ctgctttttt
     4201 tacatcatct tctccaattg aatagcttcc attcatcact ctttcgtgta cattaatttt
     4261 actgttgata tcctgaacta catcaacatt ggtttcgtac ttatcgttag taagcttaaa
     4321 gg
//
LOCUS       DAORQT010000124         4277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3677_length_4277_cov_8.388678, whole genome shotgun sequence.
ACCESSION   DAORQT010000124 DAORQT010000000
VERSION     DAORQT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_4277_cov_8.388678"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..913
                     /locus_tag="PK134_05410"
     CDS             29..913
                     /locus_tag="PK134_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB38320.1"
                     /translation="MSNQLFEIKRILIPYDFSETAALSLEHAVFMAKLLKADIYLLHI
                     LESVSFTSAFGAAFGNVDKKLESESNTKLDEIGKQIHMNNGITVKSITEVGRIYRKIV
                     DVAKRENIDIIIMGTHGVSGYKKFNVGTNTSKVVEEAACPVISVQTHSKKIGFKKIVL
                     PIDNSPQSRQKVSYALAVARSYGSHICIAGLIDFSNEDNKRMFKLKVDQVEEWLLQHG
                     VSVEKKFVEGDNLAKMTMQTAEELDADLIVIMTEQEPSITGFFLGTYATQVVNHSKIP
                     VMAVHPSESDGSTITVGY"
     gene            1003..2682
                     /locus_tag="PK134_05415"
     CDS             1003..2682
                     /locus_tag="PK134_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRB38321.1"
                     /translation="MPSFKGSLRFIFKFLLWTAGILIILFLLFLWRIQIPDPSISSKL
                     KPDDLHRIQIGVDSYRVGNNWLRKNQQGIWEMYIEGNDYERGVVYGVLAKELIEKQEE
                     YFVDQINEIIPRKIYLQFIKMFVAWFNRDIYKYIPAENLREIYGISLSFSDKYDYIGP
                     KYYRILNYHAAHDIGHALTDFQLVGCTSFAANDSLTDDHKLLIGRNFDFYMGDDFARN
                     KLLLFVKPDSGFSFVSYSWAGLTGVVSGMNEKGLTVTINAAKSDIPTTAKDPISLLAR
                     EILQHASNIKQAIAIAEKRETFVSESLLIGSAQDSTAALIEKTPTRMDVYHSPGELLV
                     CSNHYQGAELKNDPHNISNIELSDSKFRFDRITELLKENYPLSITSAVSVLRNKFGMH
                     NENIGYGNPKAINQLIAHHSILFKPQSSDFWVSTMPYQEGVYVHYNLTDVLQGKIPDS
                     DSLNIPESDFVKSNDLKKFEAFKIIKQRIYKHNTFGVNLHLTEAEIKQYIDNNPMSYV
                     TFMSLGDYFYHEKNYAMATLYYNEALKREVASKAEEEKIQKMIESCTEKSK"
     gene            2682..3968
                     /locus_tag="PK134_05420"
     CDS             2682..3968
                     /locus_tag="PK134_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB38322.1"
                     /translation="MKQLGEIEFAGLDAIRKMQQQKLSELLQYVNAKSAYYKEVFRQH
                     NIKFDSITLDNLNELPFTTKDDLATQNDSFLCVDKSFIADYVTTSGTLSDPVTFYLTS
                     ADLDRLAYNEAISLQCADGSSADIFQMMTTIDKLFMAGLAYFLGIRKLGAASIRVGPG
                     TPQAQWDYIQRFKPTTLIAIPSFIPQLLQYAAERKINFQSTSVKKIVCLGEPVRNADF
                     SLNELGKRITSLWDVKLYSTYASTEMSSAFTECGYGNGGHAHPELLILEVVDDNGLQV
                     RNGELGEVVVTTLGVEGMPLIRFKTGDLCHAHYDLCKCGRYTTRLGPVMGRKQQMIKF
                     KGTTLFPQIIFNVLNMMPQIELFQVEVKRNEFNNDSITILLSKELHSDAFEHEISSLF
                     KSKIRVTPAFRYIDTEELQQKVFKTDKRKSEKIIYI"
     gene            3994..>4277
                     /locus_tag="PK134_05425"
     CDS             3994..>4277
                     /locus_tag="PK134_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38323.1"
                     /translation="MKKTYLTLIFSFIMLNVFSQEKMNELFKNNVKLIFMGYDFSQAR
                     FIGTDGFTDPAAIKDKFLSAWNNTFVAEYEKFSLQSAFKLNHLLLSTHNRT"
BASE COUNT         1425 a          718 c          806 g         1328 t
ORIGIN      
        1 atacgtacta tcataaatcc attttcctat gagtaatcaa ttatttgaaa tcaagagaat
       61 tttaattcct tatgattttt cagaaacagc agcgttgagc ttggagcatg cagttttcat
      121 ggccaaatta ttaaaggcag atatttatct gctacatatt cttgaatctg tatcttttac
      181 ttcggcattt ggtgcagcgt ttggaaatgt agataaaaag ttagagtctg aatcgaatac
      241 taaactcgat gaaatcggca aacaaatcca catgaacaac ggaataaccg taaaatctat
      301 tactgaggtt ggaaggattt atcgcaaaat tgttgatgtt gccaaacgtg agaacattga
      361 cattataatt atgggaacac atggtgtttc aggatataag aagtttaatg ttggaactaa
      421 caccagtaag gtagttgaag aagctgcatg tccggtgatt tctgtacaga cacacagcaa
      481 aaaaatcggc tttaagaaaa ttgttttacc aattgataat tcaccgcagt cacgtcagaa
      541 ggtaagctat gcattagcag tagcccgctc ttatggctcg catatttgta ttgccggatt
      601 aatagacttc agcaatgaag acaataagcg gatgtttaaa cttaaagttg atcaggttga
      661 ggaatggctt ctacaacatg gtgtaagtgt tgagaaaaaa tttgttgaag gtgataacct
      721 agcaaaaatg actatgcaga cagcagaaga gttggatgcc gatttaattg taataatgac
      781 agaacaagaa cctagtatta caggattttt tctgggcacc tatgcaacac aggttgttaa
      841 ccacagcaaa attcctgtta tggcagtgca tccttcagaa tcggacggca gtacaattac
      901 tgtaggatat taaaaaacaa atttcatgat ttacacatgg ggcgcttgaa gagcgcccca
      961 tgtttttatt accttcgcca atcagaattt taattactat aaatgccatc atttaaaggt
     1021 agtctgcgtt ttatttttaa gtttttactt tggactgcag gaatacttat tatattattt
     1081 cttttgtttt tatggaggat tcagattccc gatccttcta taagtagtaa acttaaacca
     1141 gatgaccttc atcgcattca aataggagtt gacagctatc gtgttggcaa caactggttg
     1201 cgcaaaaatc agcaaggcat ctgggaaatg tatattgaag gtaatgacta tgaaagaggt
     1261 gtagtttatg gtgtgcttgc aaaagagtta attgaaaaac aagaagaata ttttgttgat
     1321 cagatcaatg aaataattcc acgtaaaatc tatcttcagt ttatcaagat gtttgtagca
     1381 tggttcaatc gcgatattta caaatatatt ccggctgaaa atctgcgtga gatttatggt
     1441 atttcacttt ctttttctga caagtacgat tacataggac ctaaatatta tcgcatcctc
     1501 aattatcatg cagcacacga cattggtcat gctcttaccg attttcaatt ggtaggctgt
     1561 acttcatttg ctgccaacga cagtcttact gacgatcata aattattaat cggcagaaat
     1621 tttgattttt acatggggga cgattttgca cgaaacaaac tcttactttt tgtaaagcct
     1681 gatagtggtt tttcttttgt ttcttattcg tgggcaggac ttacaggagt tgtttccggt
     1741 atgaatgaaa aaggacttac tgttaccatc aatgctgcaa agtctgatat cccaacaact
     1801 gcaaaagatc ctatttcact tttagcacgc gaaattttac aacatgcatc aaacataaag
     1861 caagcgattg ctattgcaga aaagcgcgaa acttttgttt cagaatcgtt gcttattggt
     1921 tcagcgcagg atagtacggc tgccttgata gaaaaaacac caacaagaat ggatgtatat
     1981 cattcacccg gtgaattatt ggtttgttcc aatcactatc agggtgctga attgaaaaat
     2041 gatccacaca acatttcaaa tattgaactt agcgacagta aattccgctt tgacagaata
     2101 acagaattgc taaaggagaa ttatccatta agtataacat cagcagtttc tgttctgcga
     2161 aataaatttg gaatgcacaa tgaaaatatt ggctacggta acccaaaggc aattaatcag
     2221 cttatagcac atcacagtat tttattcaaa ccacaaagca gcgatttttg ggtttctaca
     2281 atgccttatc aggaaggtgt ttatgttcac tataatttaa ctgatgtttt acagggtaaa
     2341 attcccgatt ctgactctct gaatattccg gaaagtgatt ttgtaaagtc taacgattta
     2401 aagaagtttg aagcttttaa aattatcaaa caacgaatct acaaacacaa cacatttggt
     2461 gtaaatctgc atctgactga agccgaaata aaacagtata tcgataataa ccctatgagt
     2521 tacgttacct ttatgagctt gggtgattat ttttatcatg agaaaaatta tgcaatggcc
     2581 acactttatt acaatgaagc attaaaacgt gaagtagcat caaaggcaga ggaggaaaaa
     2641 attcaaaaga tgatagaatc ctgtactgaa aaatcaaaat aatgaaacag cttggtgaaa
     2701 tagaatttgc aggtcttgat gccattcgaa aaatgcagca acaaaagctt tctgaactgc
     2761 tgcaatacgt taatgccaaa tcagcttatt ataaagaagt cttcaggcaa cacaacatta
     2821 agtttgacag cattactctc gacaatctga acgaattgcc atttacaact aaggatgacc
     2881 tggcaacaca aaatgattct tttttatgtg ttgataaaag ttttattgca gattatgtga
     2941 ctacatcagg tactttgagc gatcctgtta cattctatct gacatcggca gacttagaca
     3001 gattggcata taacgaggcc atatccttgc aatgtgctga tggcagcagt gcagatattt
     3061 ttcagatgat gactaccatt gacaaattgt ttatggcagg attagcctat tttttaggta
     3121 tcagaaagtt gggtgctgcc agtatcagag tagggccggg aacacctcag gcgcagtggg
     3181 attatattca acgtttcaag ccaacaacat taatagccat accatcattt attccacaac
     3241 tgttgcaata tgcagctgaa agaaaaatta attttcaatc aacctcggta aaaaaaatag
     3301 tttgtcttgg agagcctgtt cgtaatgcag atttttcatt gaatgaacta ggaaaaagaa
     3361 tcacttcgct ttgggatgtg aaattgtatt caacctatgc atcaacagag atgagttctg
     3421 catttacaga atgtggctat ggcaatggag gtcatgcaca tcctgagtta ttaattcttg
     3481 aagttgttga cgataatggt cttcaggtac gtaatggcga attgggcgaa gttgttgtca
     3541 caacccttgg tgtagaaggt atgcctttaa tcagatttaa gaccggtgat ttatgccacg
     3601 ctcattatga tctgtgtaaa tgtggccgat atactacccg cttaggtcct gttatgggta
     3661 gaaagcaaca gatgattaag tttaaaggca ctacattgtt tccacaaata atttttaatg
     3721 tactcaatat gatgccgcaa attgaattgt ttcaggttga agtcaaacga aatgaattta
     3781 ataatgattc aataacaatt cttttgtcaa aagagttaca ttctgatgca tttgaacatg
     3841 aaatttcttc attattcaag tccaagataa gagtaactcc ggcattcagg tatattgata
     3901 ctgaagagtt acagcaaaaa gtttttaaga cagataaacg taaatccgaa aaaataattt
     3961 acatttgaaa aattaatttt aataaaatag aaaatgaaaa agacctacct tactttaatc
     4021 ttttcattca tcatgctcaa tgtattttca caggaaaaaa tgaatgaact ctttaagaac
     4081 aatgtgaaac tcatttttat gggttatgac ttttcacaag cgcgatttat cggaacagac
     4141 ggatttaccg accctgctgc catcaaagac aagtttcttt cggcatggaa taatactttt
     4201 gttgcagaat atgaaaaatt cagtttgcaa agtgccttta aacttaatca tctgttgctt
     4261 tctacccata acaggac
//
LOCUS       DAORQT010000125         4238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3730_length_4238_cov_10.181688, whole genome shotgun sequence.
ACCESSION   DAORQT010000125 DAORQT010000000
VERSION     DAORQT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_4238_cov_10.181688"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PK134_05430"
     CDS             complement(<1..1491)
                     /locus_tag="PK134_05430"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38324.1"
                     /translation="MKRLFFFILFIGIISNVSAQITASTTSGCVPLVNVNFTSPAGLT
                     NIDWDFGDMTSSNLPNPVHTFFTAGSFTVVCTGLLSGNAFIDSIKINVFRKPFTSLTA
                     TPPLSGCVPLTVHFNDSSVGGGGTAIVSRQWAFGDGATNVGNNSSPTNIYSLAGSFTV
                     SLKVTDANGCDTSISVPNYINTSIQPNAVITTVPSPPIGCTPPFTVAFSASASTSNST
                     TGTGLTYFWNFGGGNTSTVVTPPSINYPTSGTYNISLTVTDNNNCPKTVTTQVVINPP
                     LASFFAVGAVNDTVCPTVTFRNQSIGTNPFYNYGDGTSGVDSIHTYAPGTYQVTLQVL
                     NGICTDDTTITIHVENVVANFTSTPSYSCSWPFTVQYTNITSGAGTYQWIFGDGTTSN
                     LANPSHTFYRPDTNQYTIYDYYYYTDQLTFTSVHGCSATVAKQKNDTVFSITARFMPD
                     KSIGCAPMTVTFSDSSRSRETIVNYTYLFGDGTQTTGSNGVVTHTYN"
     gene            1632..2483
                     /locus_tag="PK134_05435"
     CDS             1632..2483
                     /locus_tag="PK134_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRB38325.1"
                     /translation="MKAATLIYVILFSCLFAVSDSNAQGLCGTVIDTAKMQAEAAANL
                     NSSQNQRGLFFPVNCLNKTLSITAYIFRDSLAADGITQASILSALQILNNDFAPMCLS
                     FSICEFVYVNNYNYNDFYALKHENEVYNLYYTPNTINVYFSNTLYDNTNSSVCGYAHF
                     PGGRDMIFLNKSCVGDGKTFSHEMGHFFGLYHTFETANGVELINGTNCLVAGDLICDT
                     PADPNGLNGADCQMLPYLPDPSGNWYVPQIGNIMSYYSPGCKCGFTTQQFNWMIQQYL
                     TNRNYLW"
     gene            2517..>4238
                     /locus_tag="PK134_05440"
     CDS             2517..>4238
                     /locus_tag="PK134_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB38326.1"
                     /translation="MKSRKTNQFILIAAITALLSANVFAQDKAKSTTTPVKVTSVEGI
                     TEYKLSNGLRVLLFPDPSKSTITVNITYLVGSRMEGYGESGMAHLLEHMVFKGSTNHT
                     NIPQELTAHGARPNGTTWYDRTNYFETFNATEENLRWALSLESDRMVNSFINKKDLES
                     EFSVVRNEFERGENSPSSVLEERVMSTAYLWHNYGKSTIGSKEDIERVPIENLKAFYH
                     KYYQPDNAVLTIAGKIDEQKTLQLVSEYFSPIPKPERKLQETFTVEPAQDGERNVVLR
                     RVGDVQVASCGYHIPAGAHADYAAIEVLIEALTNEPSGRLYKALVETKKATSTSGYSF
                     QLKDPGYAYFYAEVLKDKPLDTVMNTMKGIFDNLSKNPITNEEAERGKNAILKQLTLM
                     FNNSDRVGLSLSEFIAQGDWRLYFIYRDALEKVTTADVNRVAAEYFKPSNRTTGLFIP
                     DATPDRAVIPASPDVNALVKDYKGKKALAEAEAFDPSPTNISNRIKQATISGGAKYTL
                     LKKTTRGGNVSVNITLRMGNAAVMQNKGTAASFTASMLRRGTKNKSFQQINDEIDKLK
                     SNVGINGN"
BASE COUNT         1383 a          757 c          866 g         1232 t
ORIGIN      
        1 gttgtaggtg tgtgtaacca caccattgct tcctgtagtt tgtgtgccat cgccaaataa
       61 ataggtgtaa tttacaatag tctcacgaga acgactcgaa tcagaaaaag taacagtcat
      121 tggagcacag ccaattgatt tatcaggcat aaaacgagca gtgatactaa acacagtgtc
      181 gtttttttgt tttgcaacag tggcagaaca accatgtaca ctcgtaaaag tcagctgatc
      241 ggtgtagtag taataatcat aaatggtgta ttggtttgta tcaggacgat aaaaggtatg
      301 acttgggttt gcaagatttg atgtggtgcc atcaccaaaa atccattgat aggtacctgc
      361 accagaagtt atattggtat attgaacagt aaatggccaa ctgcagctgt aacttggtgt
      421 tgaggtaaag ttggcaacaa cattttctac atgaatagta atggttgtat cgtcagtaca
      481 aataccattt aatacctgaa gtgttacctg ataggttccc ggtgcatagg tatgaatact
      541 gtcaacgcct gaagttccat caccgtagtt atagaatgga ttggtaccaa ttgattgatt
      601 tctgaaagtt actgtaggac aaaccgtatc attaactgca ccaacggcaa agaatgatgc
      661 tagtggagga ttaatgacaa cctgagtagt aactgtctta ggacaattgt tgttatcagt
      721 aaccgtaagt gaaatgttat aagttccact cgtgggatag tttattgaag gaggtgttac
      781 aaccgttgat gtatttccac caccaaaatt ccaaaagtag gtaagccctg ttcccgttgt
      841 agaatttgat gtagatgctg aggcagaaaa ggcaactgta aatggcggtg tgcaacctat
      901 tggcgggctg ggcactgttg taattacagc gttaggttga atgcttgtat taatataatt
      961 tggaacagaa atagaagtgt cgcagccatt ggcatcagtc acttttagcg agactgtaaa
     1021 acttccggca agagaataaa tgtttgttgg tgaagaattg tttcctacat tggttgcgcc
     1081 atcaccaaat gcccactgcc tcgaaacaat tgcagtacca ccaccgccaa cagatgaatc
     1141 attaaaatga acagttaatg gaacacaacc tgaaagcgga ggtgttgccg taagcgatgt
     1201 aaatggtttt cggaagacat ttatttttat tgaatcaata aaagcatttc ctgagagcaa
     1261 gcctgtgcag acaacagtaa aacttcctgc tgtaaaaaaa gtatgtaccg gattaggcag
     1321 gtttgatgag gtcatatcac caaaatccca atcaatattt gtcagccctg caggcgaggt
     1381 aaaattaaca tttaccaaag gcacacagcc acttgtggtg ctcgctgtaa tttgtgcact
     1441 gacatttgat ataatgccta taaaaagaat gaaaaagaat aatcttttca agatgcgtga
     1501 agaaaaaagt gaaatgttaa tttttgtttt caaatttacg gcttttcggt aacgatgtaa
     1561 aaaatttata tgtttgtagt gtctgtatgc ggtaagatga attttcaatc actcaaaagt
     1621 atagcctttg catgaaagca gcaactttaa tttatgtcat tttattttcc tgcctttttg
     1681 cagtatcgga tagcaatgca caaggcttgt gcgggactgt gattgataca gctaaaatgc
     1741 aagccgaagc agcagctaat ttaaattctt cacaaaatca acgcgggctt tttttccctg
     1801 taaactgttt gaataaaaca ctttctatca ctgcctatat ttttcgagat agtttagctg
     1861 ccgatggcat cacacaggca tcaattttgt cggcattgca aattctgaat aatgactttg
     1921 cacctatgtg tttgtcgttc agcatctgtg agtttgtgta tgtcaacaat tacaattaca
     1981 atgattttta tgcattgaag catgaaaatg aagtttataa tttatactat actccaaata
     2041 ctataaatgt ttatttcagc aacacattat acgacaatac aaactcatcg gtttgtggat
     2101 atgcccattt tccgggtgga cgcgatatga tttttctgaa taaatcttgt gtaggcgatg
     2161 gcaaaacttt ctcgcatgag atgggacatt tttttggact ttatcatacg ttcgaaactg
     2221 ccaatggagt tgaattaatt aatggcacaa attgtctggt agccggtgat ctgatatgcg
     2281 atacacctgc agaccctaat ggattaaacg gtgcagactg tcagatgctt ccttatttgc
     2341 ctgacccttc cggcaattgg tatgttccgc aaatcggcaa catcatgtca tactattctc
     2401 ccggttgcaa atgtggtttt actactcagc aatttaactg gatgatacaa caatatctca
     2461 ccaacagaaa ctacctttgg taaaagaatt ttcttattag ttcaattcaa atcattatga
     2521 aatcacgaaa aacaaatcag tttattctga tagctgccat tacagcattg ttatcagcaa
     2581 atgtttttgc acaagacaaa gccaaatcaa caactacacc ggttaaagtt acttccgtag
     2641 aaggaattac ggaatataaa ctcagcaatg gcttacgtgt attactgttt cccgatccat
     2701 caaaatctac catcacggta aacattactt acttggtagg ttcgcgcatg gaaggttatg
     2761 gagaatcagg aatggcgcat ttattagaac acatggtgtt taaaggttct accaaccata
     2821 caaatattcc gcaggaactt actgcacatg gtgcaagacc aaatggaact acctggtatg
     2881 acaggacaaa ttattttgaa acatttaatg caacagaaga aaatcttcgt tgggcattaa
     2941 gcttggaaag tgacagaatg gttaattcat tcatcaataa aaaagatctt gaatctgaat
     3001 tttctgttgt gcgaaatgaa tttgaaagag gagaaaacag tccttcaagt gtattggaag
     3061 agcgtgtaat gtcaactgct tatctatggc ataactatgg aaagtcaacc atcggatcaa
     3121 aagaagatat tgagcgtgtt cctattgaaa acctgaaagc attttatcat aaatactatc
     3181 agccggataa tgcagtactt actattgccg gaaaaattga tgagcaaaaa acattacaat
     3241 tagtaagcga atattttagt cctattccta aacctgaacg taaattgcag gagacattta
     3301 cagtagaacc tgcacaggat ggcgaacgta atgttgtttt acgcagagtt ggtgatgtgc
     3361 aggttgcctc ttgcggatat catatccctg caggtgctca tgccgactat gcagcaattg
     3421 aagttttgat agaagcatta acaaacgaac cttcgggaag attatataaa gcattggtgg
     3481 aaacaaaaaa agctacaagt acatcgggct attcatttca gcttaaagat ccgggatatg
     3541 cctatttcta tgcagaagta ttaaaagaca aacctcttga cacagtcatg aataccatga
     3601 aaggtatttt tgataacctg agtaaaaatc ctattacaaa cgaagaggca gagcgtggca
     3661 agaatgccat tctcaaacaa cttacactga tgtttaacaa cagcgatagg gtagggcttt
     3721 cactaagtga atttattgca caaggcgact ggcgtttgta ttttatttat cgtgatgcat
     3781 tagagaaagt tactactgcc gatgtaaaca gagtggctgc agagtatttt aaaccaagca
     3841 accgtactac aggacttttt attcctgatg caacaccgga cagagctgtg attcctgcat
     3901 cacccgatgt taatgcattg gttaaagatt ataaaggcaa aaaagcgttg gcagaagcag
     3961 aagcatttga tccttcgcca acaaacatca gcaacagaat aaaacaggca actatttccg
     4021 gtggtgcaaa atatacctta ctgaaaaaaa ccactcgtgg tggcaatgtc agtgtaaaca
     4081 ttacattgag aatgggaaat gctgcagtga tgcaaaataa aggtactgct gcatcattca
     4141 ctgcttcgat gttgcgcaga ggaacaaaga ataaatcatt tcagcaaatt aatgatgaga
     4201 ttgacaaact taagtctaat gtaggcatta acggtaac
//
LOCUS       DAORQT010000126         4181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3809_length_4181_cov_7.961464, whole genome shotgun sequence.
ACCESSION   DAORQT010000126 DAORQT010000000
VERSION     DAORQT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_4181_cov_7.961464"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3294
                     /locus_tag="PK134_05445"
     CDS             <1..3294
                     /locus_tag="PK134_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38327.1"
                     /translation="VLCYGNSNGSVQVQVNGGTGAINYLLSNGDVSSSGIFDSLTWGN
                     YSVIITDANGCSVTGNAIVAQPQPLNFFVNTTASTCGNSNGVITVNSITGGTGVCVFS
                     LDSGLTNQSQNQFVNLNAGNYSVLATDSNGCSLQSNVLLANIAGPLFNALNITDALCS
                     GAMNGSITVSPQGGTGPFLYSADSGFTYQAGNIFSALSAGNYSLIIQDANGCLTDSIA
                     TIGEPLPLNYQVASAVATCGSSNGTITLNATGGNGSYQYSINNGITFQSTGTFGGLIP
                     AGYPVVVTDLNGCLDSGLVSVADAPSPVLTLVSVNDATCNGFTDGVVSVTSQSGTLPI
                     QYSINGGTWQQSGSFANLSAGNYSVIVQDSYGCTDTVTALIAEPLVIALQTSTIPVIC
                     NGQSNGQTAVTVTGGTMPYSYQWNAVATTASVNNLSAGTYSVVITDANNCTSSATAVV
                     TEPVVLQMTLATTPVTCFGYSNGIINTTASGGTGNLQFSIDTGATYQSSTIFSSLTFG
                     NYNIQVTDANGCTLSVPVSMTQPSAVALTSNTVDVKCFGGNDGSLTANANGGTAPYQF
                     VINNGNPQAGTTFNNLTAGNYVISVTDAMGCLKTISDSVGQPTALSAVASGQNVLCNG
                     QSNGAVNVIITGGVQPYMYQWTGGYTTSLVNNLPQGNYQVTVTDFNGCTIHSSASITQ
                     PTAINLGLTPDPTICIGQSASVTANANGGTPPYVFKWNTGHVGATLQASPVITTTYNV
                     FLTDSNGCNSPLRYANINVNPALALVMTDDDTLCEGQSTTLTATPSGGNGGPYIINWN
                     GQTQPATSITVAPTQTTNYVATLTDGCTLMPATANAVVVVNPLPQVVFVPLITEGCVP
                     LIVDFQNMSSNGASWQWTFGDGDKDTLINPTHTYTQPGTYSVGLYAVSNAGCENQLVL
                     DDAIQVYENPQAAFTSNRVEASILDPWFEFRNQSIGASTYLWDFGDQRTQSTEINPMY
                     VYGDTGTYQIIMTATSEHQCSDTAYGEVHVSGAVTVYIPNAFTPNSDGRNELFSVSGI
                     GYTELQMIIFDRWGNMLFNQTSSNPGWNGMNMFNNSMCQQGVYVYKVIVKNIFNKQQE
                     FKGTVSLLR"
     gene            complement(3535..>4181)
                     /locus_tag="PK134_05450"
     CDS             complement(3535..>4181)
                     /locus_tag="PK134_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB38328.1"
                     /translation="LKANSTTMLKQLSDLSVLTTTQSESIRKSLENINAKDIFIQDLQ
                     REMSRKDSLNMALVMNLKGALKDVNDKDVEIKVEGSAVFISISDKMLFRSGSYEITPK
                     AKEVLGKVAEVIKAQPEVQFMVEGHTDNKSIHTAFIKDNWDLSVLRATSVVRVLQKNY
                     GVDPSRIIAAGRSEYVSLQPNTTAEGRAMNRRTRIVILPQLDQFFKLMEPKTKP"
BASE COUNT         1230 a          750 c          868 g         1333 t
ORIGIN      
        1 gttttatgtt atggcaatag taatggatca gttcaggttc aggtaaatgg tggcacagga
       61 gcaataaact atttattaag caatggtgat gttagttcgt caggaatttt cgattcatta
      121 acgtggggta attattcagt aattattacc gatgcaaacg gttgtagtgt tacaggaaat
      181 gcaattgttg cacaaccaca gcctttgaat ttctttgtta acacaacggc atcaacctgt
      241 ggtaatagca atggtgtaat cacagtaaat agtataacag gaggaacagg agtttgtgtt
      301 ttctctttag atagcggatt gaccaatcag tcacaaaatc aatttgtgaa tttgaatgcc
      361 ggaaattatt ctgtgctagc tacagattca aatggttgtt cattgcaatc gaatgtttta
      421 ttggcaaata ttgcaggtcc tttatttaat gcattgaata ttacagatgc actgtgttcc
      481 ggtgccatga atggctcaat aaccgttagc ccacaaggtg gcacaggacc gtttttgtat
      541 agtgcagata gtggttttac ttatcaggca ggtaatattt ttagcgcatt atcagcagga
      601 aattatagtt taattattca ggatgccaac ggatgtttaa cagacagtat tgcaacaata
      661 ggtgaacctt tgcctctgaa ctatcaggtt gcttcggcag tggctacatg tggtagcagt
      721 aatggaacaa tcacattaaa tgccacaggt ggtaatggaa gttatcagta tagtatcaat
      781 aatggaataa catttcagtc aacaggaact tttggaggtt tgattcctgc aggttatcct
      841 gtagtggtaa cagatttgaa tggatgtttg gacagtggat tggtaagtgt tgcagatgcg
      901 ccatcaccag tattaacttt agtatctgtt aatgatgcaa cctgtaacgg atttacagac
      961 ggtgttgttt ctgttacatc acagagtggt acattgccaa ttcagtacag tataaacgga
     1021 ggaacttggc agcagtcagg tagctttgct aatttatctg caggaaatta ttcagtaata
     1081 gttcaggata gttatggctg tacagacaca gttactgcgt taatagcaga accgttagta
     1141 attgcattgc agacttcaac aatacctgtt atttgtaatg gacaaagtaa cggacagact
     1201 gcagtgacag ttaccggtgg tacaatgcca tattcttatc aatggaatgc ggtagcgacc
     1261 acagcttctg tgaataatct ttctgccgga acatattccg ttgttattac tgatgccaac
     1321 aattgtacat catcagcaac tgctgtagta acagagcctg tggtattaca aatgacattg
     1381 gctacaacac cggtaacatg ttttggctat agcaatggta tcattaatac aacagcatca
     1441 ggggggacag gtaatctgca attctctata gatacaggag caacctatca gagttcaact
     1501 atattttcat cattgacttt tggaaattat aatatacaag ttaccgatgc caatggctgt
     1561 acattatctg ttccggttag tatgacacag ccttctgctg ttgccctgac ttcaaataca
     1621 gttgatgtga aatgttttgg tggcaatgat ggaagtttaa ctgcaaatgc aaatggagga
     1681 actgcacctt atcagtttgt tattaataac ggtaatccgc aagcaggtac aacatttaat
     1741 aatctgacag caggtaatta tgttatttca gttacagacg ctatgggttg tttgaaaaca
     1801 atttcagact ctgtagggca gccaacggct ttaagtgctg ttgcttccgg gcagaatgtt
     1861 ttatgtaacg gtcagagcaa tggcgcagtt aatgttatca ttaccggagg tgttcaacct
     1921 tatatgtatc aatggacagg aggttacacc acatcacttg taaacaattt gcctcaggga
     1981 aattatcagg taacggttac agattttaat ggatgcacta ttcattcatc ggcaagtata
     2041 acacaaccaa ctgcgataaa tttaggatta acccctgacc cgactatttg cattggacaa
     2101 tctgcatcgg tgacagcaaa tgctaatggt ggtacgcctc cttatgtgtt taaatggaat
     2161 acaggacatg ttggtgcaac tttgcaggct tcacccgtaa ttacaacaac atataatgtg
     2221 tttttaactg atagcaatgg ttgtaattcg cctttgcgtt atgcaaatat taatgtcaat
     2281 cctgccttag ctttggtaat gactgacgat gacaccttat gcgaaggaca aagcactacc
     2341 ttaactgcaa caccttccgg tggcaatggc ggaccctata tcatcaactg gaatgggcaa
     2401 acacaacctg caacatcaat tacagttgca ccaactcaaa ccaccaatta tgttgctact
     2461 ctgactgatg gatgtacttt aatgccggct acagcaaatg cagtagttgt tgttaatccg
     2521 ttgccacaag tagtttttgt tccattgata acagagggtt gcgtgccgct gattgttgat
     2581 tttcagaata tgtcttctaa tggtgcttca tggcaatgga cttttggcga tggtgataaa
     2641 gacactttaa taaatcctac gcacacctat acacagcccg gaacttactc agtgggcctt
     2701 tacgcagtta gtaatgcagg atgcgaaaat caacttgttc ttgatgatgc cattcaggtt
     2761 tatgaaaacc cgcaagctgc atttacaagc aacagggtag aagcatcaat tcttgaccca
     2821 tggtttgagt tcagaaatca aagtattggg gcttccacat atctgtggga ttttggtgat
     2881 cagcgaacac aatcaacaga aatcaatcct atgtatgttt atggtgacac cggaacctat
     2941 cagatcataa tgactgccac ctctgaacat cagtgtagcg atactgccta tggcgaagtg
     3001 catgttagtg gtgctgttac tgtgtatatt ccaaatgcat ttacgccaaa tagtgacggg
     3061 cgtaatgaat tgttttctgt ttcaggtatt ggctatacag aattgcagat gataattttt
     3121 gaccgatggg gaaatatgtt gtttaatcag acttcttcaa atccgggatg gaatggcatg
     3181 aatatgttta ataattcaat gtgtcagcaa ggagtttatg tttataaagt gattgtaaaa
     3241 aacatattca acaaacaaca ggagtttaaa ggaactgtaa gtttattgag gtaaattgta
     3301 agtgaaaaaa aagctgctct attgagctgt cttttttgtt attgtctgta ttgtacaatg
     3361 tctttcatta ttacaaatca tgcatttttg ttttgacctg acccttaggt ttgtgcaatc
     3421 ataaactctc ccgcatttca ggggacgttg aatggacaaa aggtaaaaat atatgataaa
     3481 aatttagctc ttcacaaatt tttgcgaagg gcttttgctt taatttaagt attgttatgg
     3541 cttagttttt ggctccatca atttaaagaa ctgatcgagt tgtggcagaa taacaattct
     3601 ggttctgcgg ttcatggcac gtccttctgc agttgtgtta ggctgaagac ttacatactc
     3661 actgcgtccg gctgcaatga tgcgtgatgg atcaacacca taattttttt gtaaaacacg
     3721 aactacagac gtagcacgca gtacacttaa atcccaattg tcttttataa atgctgtgtg
     3781 aatagattta ttgtcagtat gaccttcaac cataaactgc acttcaggtt gggctttgat
     3841 tacttctgct acttttccta aaacttcttt tgcctttggt gtaatttcat aacttccact
     3901 tctgaaaagc attttatcgg aaatagaaat aaacacagca ctgccttcaa cttttatctc
     3961 tacatcttta tcgttaacat ctttcaatgc gcctttaagg ttcattacta atgccatatt
     4021 caaagaatct tttcttgaca tttctcgctg caagtcctga ataaaaatat cttttgcatt
     4081 aatattttcg agcgatttgc ggatactttc actttgtgtg gttgtcaata cagaaaggtc
     4141 tgatagctgt ttcagcattg tagtactgtt ggctttaaga t
//
LOCUS       DAORQT010000127         4179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3811_length_4179_cov_7.141125, whole genome shotgun sequence.
ACCESSION   DAORQT010000127 DAORQT010000000
VERSION     DAORQT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3811_length_4179_cov_7.141125"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1017)
                     /locus_tag="PK134_05455"
     CDS             complement(31..1017)
                     /locus_tag="PK134_05455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HRB38329.1"
                     /translation="MAMLLELTNKGIYCPQADVYIDPHRRVENAIITHAHSDHARPGS
                     SHYLAHKDSEAMLRMRLGEQISLRTVAYGESFTINGVKFSLHPAGHIIGSAQIRVAYK
                     DEVWVAAGDYKLHDDGFCPVFEHLKCNVFITESTFGLPIYKWQPQQQVMQEIDTWINK
                     NNQQQITSVLMGYSLGKMQRLIKHLNHFDFGIYGTPDVVAVNDRLREAGCNLPYVKPL
                     KSNPEKENLKGALIFASPSGARSALFKKLDNYSVGYCSGWMLHGKPKYLSHVDRGFAL
                     SDHADWDELNTAVKESGAEKIFVTHGSTASFSKWLCDNGYDAKEIELMKGAG"
     gene            1119..2495
                     /locus_tag="PK134_05460"
     CDS             1119..2495
                     /locus_tag="PK134_05460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB38330.1"
                     /translation="MNQTMRLILLGLLVFASRWFFLDAGYGAEEDAWGYAVNALAMDS
                     TNSYQYSRLPGHPVPEWVYYFLPVKHSFAFNLCTAMLSTIAVLLFYLICKKYKLNAIA
                     GALMLAFTPVFFIQSTNAMDYNWSLCFMLAALFFAGKRNMILTSIMLGLAIGCRITAV
                     LFIIPISIYLLKADRSWKLVLQLLFLSGVVSALLYLPLILKYNLAFLTYVNQFGYPQI
                     LKTIYKASFGVWGTLGFVVIIIAFSYALQNIFNGMHSRKYKPILLCAASTLIIYTLLF
                     FYEPHKSAYLIPVIPFVFLIILIALQDKVWPVVITASLVISCFLGGINLADSHRSALP
                     GKYAIVRKAGSQLIAFDPLSGNVTDDHSKRIQRIKYAGSVVEQAMQLKGKNALICGYW
                     INMVSILGIGQLSDKQTLLHYADEQELIKLKNANTEIYYIPGQDSFNDVCFKNVFTRK
                     WAKSLFPQ"
     gene            2656..3483
                     /locus_tag="PK134_05465"
     CDS             2656..3483
                     /locus_tag="PK134_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRB38331.1"
                     /translation="MIKKPKVLFVSQEIFPYLESSTIGNIARHLPQGTQEKGKEIRTF
                     MPRFGVVNERRNQLHEVIRLSGMNLIIDESDHPLIIKVASIQAARMQVYFIDNEEYFQ
                     RKHTTHDADNNFFKDNDDRTIFFCRGVLETVKKLGWAPDIIHCHGWMSSLIPLFVKTA
                     YREDPIFRNARVVYSVYDDEFSESFHDSYSKKLMMEGISTNDVKDIKSPNFESITIAA
                     INKSDAIIKGAEKLNTKIDNHIKKSGKLILPYQSPENYVEAYNTFYDEIMEEISIEA"
     gene            3533..>4179
                     /locus_tag="PK134_05470"
     CDS             3533..>4179
                     /locus_tag="PK134_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRB38332.1"
                     /translation="MQHFKRLWMACVVLLSISCNKPDSVGLNLLPGEDAMNVEFFDTL
                     TLQTSYKQEDSLRTDAHLLYELQATNHQFMLGKYVDPIFGETSAELFTQLKPTAVFSN
                     TNKTYDSLFLNLAYISNYGDTSALQTFEVYELTDAMYDTASYYAFSNLNYSTKVGEIT
                     FKVEDAQDSIPTSDTTKIAPRLRLRLDDVLGQKIFNSDLSDPSKFKETVKGFCIKP"
BASE COUNT         1248 a          816 c          773 g         1342 t
ORIGIN      
        1 aagcagtgag taaaaacagt tttattcttc ttaccccgcc cccttcatca gttcaatttc
       61 ttttgcatca tatccattgt cgcaaagcca ttttgaaaat gatgccgtac ttccatgtgt
      121 tacaaaaatt ttctccgcac cactttcttt aactgctgta ttcaactcat cccagtcggc
      181 atggtcgctt agtgcaaaac cacggtcaac atggctcaag tatttcggtt tgccatgcaa
      241 catccatcca ctgcaatagc caacactata gttgtcgagt ttcttaaaca atgccgaacg
      301 tgctccggat ggtgatgcaa atattaatgc ccctttcaga ttttctttct cagggtttga
      361 tttcagcggt ttaacataag gcaaattgca acctgcttcg cgcaatctgt cgttaacagc
      421 aacaacatcc ggtgttccat aaatgccaaa atcaaaatgg tttaagtgct ttatcagcct
      481 ttgcattttg ccaagcgaat aacccatcag tacagaagtt atttgttgct gattattctt
      541 atttatccaa gtatcaattt cctgcattac ctgttgttgt ggctgccatt tgtaaatagg
      601 taaaccaaag gttgactctg taataaacac attacatttt aggtgttcaa acacagggca
      661 gaaaccatca tcgtgcagtt tgtaatcgcc tgctgccacc catacttcat ctttatatgc
      721 tactcttatc tgtgcactgc caataatatg accggcagga tgcaaagaaa attttactcc
      781 gttaattgta aatgactctc cataagcaac cgtgcgtaaa ctaatttgtt cgcccaatcg
      841 catacgcagc atggcttccg aatctttatg agcaagatag tgactgcttc cagggcgagc
      901 atggtcgcta tgtgcatgtg tgatgatggc attttctact ctgcgatgtg ggtcaatata
      961 gacatctgcc tgcggacagt aaataccctt gtttgtcaac tctaataaca tagccatgac
     1021 ggtgctgcaa ttatttgtaa ttacgtaaag atataaatgt gttgttaaaa ttgaatcaac
     1081 atcatataaa aatggtcaaa acatgttact ttgcgtccat gaatcaaact atgcggctta
     1141 tccttttggg attattggtt tttgccagcc gctggttttt tctcgatgca ggttatggtg
     1201 ccgaagaaga tgcatggggc tatgccgtaa atgctcttgc aatggattca acaaatagtt
     1261 atcagtattc gcgtttgccc ggacaccctg tacccgaatg ggtgtattac ttcctgcctg
     1321 taaaacattc ttttgctttt aatttatgta cagccatgct gagtacgata gctgttttgc
     1381 tgttttattt aatctgcaaa aaatataaac tcaatgctat agccggagca ttaatgcttg
     1441 cttttactcc tgtttttttt attcaaagta ccaatgccat ggactataac tggtcgctgt
     1501 gctttatgct ggcagccttg ttttttgccg gaaaacgaaa tatgatttta acctccatta
     1561 tgcttggtct ggcaataggt tgccgcatta cagcagtgct gtttattatc cccataagta
     1621 tttatcttct aaaagccgat cgttcatgga aacttgtgct gcagcttctg tttttatcag
     1681 gagtagtgag tgcactttta tatcttccat tgatattaaa atacaacctg gcttttctga
     1741 cttatgtaaa tcaatttggc tatccgcaaa ttttaaaaac tatttataaa gcaagttttg
     1801 gtgtttgggg aacattgggt tttgtagtta ttataatagc ttttagttat gcgcttcaga
     1861 atatttttaa tggaatgcat agccggaaat ataaacccat actgttgtgc gctgcttcaa
     1921 cacttataat ttatacctta ctttttttct atgaaccaca taaatctgct tatcttattc
     1981 ctgtaattcc atttgtgttt ttaataattc tgattgcatt gcaggataaa gtttggcctg
     2041 ttgtaattac agcatcattg gtaataagtt gttttttggg aggtattaat cttgcagaca
     2101 gtcacaggtc ggcactgccg ggtaaatatg ccatagtcag aaaagcaggt tcccaactca
     2161 ttgctttcga cccattaagt ggtaatgtta ctgacgatca ttcaaaaaga attcagcgga
     2221 taaaatatgc aggaagtgtt gtagaacagg caatgcagtt aaaaggtaaa aatgctttga
     2281 tttgtggata ctggataaat atggtttcta ttctgggtat aggtcaactt tctgacaaac
     2341 agactttgct gcattatgcc gatgaacagg aattgataaa attgaaaaat gccaatacgg
     2401 agatttatta catacccggg caagacagtt ttaatgatgt ctgttttaag aatgtcttta
     2461 cacgtaaatg ggcaaaaagc ctatttccgc aataaatcaa ccccaattgc ccaatttcta
     2521 ctttttttta gtattaaaga ttaagccttt tgcctgcata tcagctactt taacaaaata
     2581 tcgctaaaat tttgccggtt caaagggaat ttttatatat ttttgcatac gtaaatttta
     2641 agagaagtag attgcatgat taaaaagccc aaggtcctgt tcgtatcgca ggaaattttc
     2701 ccttatctcg aaagttcaac aataggtaac attgcaagac atctgccaca aggcacacag
     2761 gagaaaggta aagaaatcag aacctttatg ccacgctttg gcgttgttaa tgagcgtaga
     2821 aatcaattac atgaggtaat ccgactttcc ggtatgaacc tgattattga tgaaagcgat
     2881 catccgttga ttataaaggt tgccagtatt caggcagccc gcatgcaggt ttattttatt
     2941 gataatgagg agtattttca aagaaaacat actactcatg atgcagacaa caactttttt
     3001 aaggacaatg acgaccgtac cattttcttc tgtcgtggtg ttcttgaaac ggtaaaaaaa
     3061 ctcggttggg caccggatat tattcattgc cacggctgga tgtcgagtct gattccgctg
     3121 tttgtaaaaa cagcctatag agaagaccct attttccgaa atgcaagagt ggtgtattct
     3181 gtatatgacg atgaattcag tgaatcattt catgacagtt actctaaaaa actcatgatg
     3241 gaagggattt ctactaatga tgtaaaagac ataaaaagtc caaacttcga aagcataaca
     3301 attgctgcca taaacaagtc agatgctatc attaaaggtg ctgaaaagct caataccaag
     3361 attgataatc atattaaaaa atcaggcaaa ttgattttac cataccaatc gcctgaaaac
     3421 tacgttgagg catacaatac tttctatgat gaaatcatgg aagaaatctc aatagaagcc
     3481 taagcggata ttgttcaaca ttaattattt taaaaaacca cgccttattt atatgcaaca
     3541 ctttaagcgt ttgtggatgg catgtgtcgt gcttctaagt atctcatgca acaagcccga
     3601 ctctgttggt cttaacctct tgcccggaga agatgccatg aatgttgaat ttttcgacac
     3661 attgacactt cagacatcgt acaagcagga agacagcctg cgcaccgatg cacatctgct
     3721 ttatgaactt caggctacaa accatcagtt tatgctcggc aaatatgttg accctatttt
     3781 tggtgaaact agtgctgaac tttttacgca acttaagcca acagcagttt tttccaatac
     3841 caacaaaaca tacgattcat tatttctgaa tctggcatat atttcaaact atggcgacac
     3901 ctccgccttg cagacttttg aagtttatga actgactgat gctatgtatg acacggcaag
     3961 ttattatgct ttcagtaatc tcaattacag tacaaaagtt ggcgaaataa catttaaggt
     4021 tgaagatgct caagacagca ttcctacttc tgacacaaca aaaattgccc ctcgactgcg
     4081 tttgcgcctt gatgacgttt taggtcagaa aatatttaat tccgatttaa gcgacccatc
     4141 aaaatttaaa gaaacggtta aaggtttttg tattaaacc
//
LOCUS       DAORQT010000128         4171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3823_length_4171_cov_3.954325, whole genome shotgun sequence.
ACCESSION   DAORQT010000128 DAORQT010000000
VERSION     DAORQT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_4171_cov_3.954325"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1331)
                     /locus_tag="PK134_05475"
     CDS             complement(3..1331)
                     /locus_tag="PK134_05475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB38333.1"
                     /translation="MTFRDLNLSTALLNALEDLNFSIPSTIQQRAFAPIMAGSDVLGI
                     AQTGTGKTFAYLLPLLRQWKFSKKRIPEILVMVPTRELVVQVVEEANKLCKYMDCKII
                     GVYGGTNIKTQMAAIATGADIVIATPGRLQDLLLNKTIHAKMVKKIVIDEVDEMLNLG
                     FRTQLKTIFDLLPERKQMLMFSATVNEEVQILIDYSFNTPVRIEAAPTGTPLSNIQQI
                     GYWVPNYITKINFLEHLLELDSTLTKVLIFAASKRLADDIHLRLEPKFSEQLAVIHSN
                     KAQNTRFKTVDDFESGASRILIATDLIARGLDVSAVSHVINMDVPAVSENYIHRIGRT
                     GRADKPGVSITFITEKDKENKQRVEALMGCEIPMFEIPKEVVISTQLTKEELPVISMK
                     IIEVKQPKVIAKGESHHEKLGKNKKVNIKVTREDAKKKKYGKRYVRSHRS"
     gene            complement(1321..>1995)
                     /locus_tag="PK134_05480"
     CDS             complement(1321..>1995)
                     /locus_tag="PK134_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRB38334.1"
                     /translation="QHGLTAVVGSFPPLLGNSLIRRKGFIEFSTLGNIFQNAGYQTQF
                     FHNGHNGFGDTDFFLKQGGFENLTDEKDFTNWKFRNELGVCDEDLYEKVYPEIWSKKD
                     KPKLSLILTMSNHAPHEIPNDFKASHPELKNLDKKEAAFYYSDYALGKFLDQCAQHSE
                     FKNTLFLLLADHGEIYDETDHGYKLFHIPALLLNSSRGKGTFASTCSQVDFAPTLLSE
                     ISFHDI"
     assembly_gap    1996..2043
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2044..>2683)
                     /locus_tag="PK134_05485"
     CDS             complement(<2044..>2683)
                     /locus_tag="PK134_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38335.1"
                     /translation="LVFFINAIDIFYYTQYGTRLNMMVSEGVQRYSIIFSLIWKSYPV
                     IPLLLVYSFGVFVFLHLHKRLFKSLDKAQQRNPIFIAWNCLMFFALSFMYYGPPFWYL
                     TSFSSSTAANQMASNGVYTFVKAIDQKNIFDRDIAAYEFNSVQSALNGLKEKVIKPDE
                     KSDSEYYPTLRHFLAPIPFNKKNVVIILLESFAARNVGCLGGKNISPYFDRYA"
     assembly_gap    2684..2727
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3058..3726
                     /locus_tag="PK134_05490"
     CDS             3058..3726
                     /locus_tag="PK134_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRB38336.1"
                     /translation="MLQHQLVKHIFAFSKKLLTKTKFKMKKLMLSIAIIGGAVLNMSA
                     KPETSAWAVDKSHSGVNFSVTHMMMAETDGKFKVFDGSIISTNEDFTDAQINFTVDVS
                     SVNTDDEKRDGHLKSDDFFNAEKYPQMKFKGTSFKKVDGKKYILEGELTIRDITKKVK
                     FDVVYGGTAKNPWGMTVAGFKASTTINRLDYNLKWNKALEAGGVLVGEEVTVKVNMEM
                     VKGK"
     gene            3826..>4171
                     /locus_tag="PK134_05495"
     CDS             3826..>4171
                     /locus_tag="PK134_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRB38337.1"
                     /translation="MTKTISEAIVQSKFRNEYHKAGVALAYAYNQVNSLQHNFFKEFS
                     ITMQQFNVLRILRGQSPNVCNLILIKERMIDKMSDVSRIVERLRVAGYVERCLNEKNR
                     REVEIKISKSGLK"
BASE COUNT         1346 a          694 c          784 g         1255 t
ORIGIN      
        1 agttaactgc ggtgggaacg cacataacgt tttccatatt tttttttctt tgcatcctcc
       61 cgcgttacct tgatgttcac ttttttgttt ttacccaatt tttcatggtg tgattcgcct
      121 ttggctatta cctttggctg tttcacctca ataattttca tgcttattac cggcagctcc
      181 tccttggtta attgtgtgga aataaccacc tctttcggaa tttcaaacat tggaatttca
      241 cagcccatca aggcttctac cctttgttta ttttctttgt ccttttcggt gataaaggta
      301 atcgaaactc cgggcttatc tgctcttccg gttcgtccaa ttctatgaat ataattttct
      361 gacacagcag gaacatccat attaataaca tgcgaaacag cagaaacatc caaaccgcgg
      421 gcaattaaat ctgttgctat taaaatgcga gatgccccac tttcaaaatc atccaccgtt
      481 ttaaaacgtg tattctgtgc cttgttggag tgtatcacag ccaattgctc tgaaaattta
      541 ggttccaacc ttaaatgaat atcatcagcc aaacgcttgc tggctgcaaa aatcaaaacc
      601 ttagtcaggg tggaatccaa ctccagtaaa tgttctaaaa aattaatttt ggttatgtag
      661 ttgggaaccc agtagccaat ttgctgaatg ttggagagag gagtgccggt tggagctgct
      721 tcaattctta caggagtatt aaaactgtag tcaattaaaa tttgaacttc ctcattcact
      781 gtagcagaaa acataagcat ttgttttctt tcaggaagga gatcaaatat tgtttttaat
      841 tgcgttcgaa atcccaaatt caacatttca tctacctcat caatcacaat ttttttaacc
      901 attttggcat gaattgtttt atttaaaagg aggtcttgta ggcgaccggg tgtagcaatt
      961 acaatatctg ctccggtagc aatggcagcc atttgtgttt taatattggt ccctccatat
     1021 acaccgatta ttttacaatc catgtattta cataatttat ttgcctcttc aacaacttga
     1081 actaccaatt ctcgcgtagg caccatcact agaatttcag gaattcgttt tttagaaaat
     1141 ttccactgcc gcaacagggg taaaagatag gcgaaggttt tgcctgttcc tgtttgtgca
     1201 attcccaaaa catctgaccc tgccatgatg ggagcaaaag ccctttgttg aattgtagat
     1261 ggaattgaaa aatttaaatc ttccagtgca ttcaataaag cagtgctaag gttgaggtct
     1321 ctaaatgtca tgaaagctaa tttctgaaag tagggtaggt gcaaaatcaa cttgtgaaca
     1381 agtggatgca aaagtacctt tgcctcttga cgaatttaac agcagtgcag gaatatgaaa
     1441 taatttgtaa ccatgatccg tttcatcata gatttctcca tggtcggcca gaagtaaaaa
     1501 aagggtattc ttaaattctg agtgttgtgc acattggtct aaaaatttac caagtgcgta
     1561 atctgaataa taaaaggcag cttccttttt atccaaattt tttaattctg gatgactcgc
     1621 tttaaaatcg ttaggtattt catgaggtgc atggttgctc atggttaaaa tgagggatag
     1681 ttttggctta tcctttttac tccagatttc aggataaact ttttcatata agtcttcgtc
     1741 acaaacaccc aattcatttc gaaatttcca attcgtaaaa tctttctcat ctgttaaatt
     1801 ttcaaaacct ccttgcttta aaaagaagtc ggtatcacca aaaccattat gtccgttatg
     1861 aaaaaattga gtttggtaac cagcattttg aaaaatattc cctaatgtac taaactcaat
     1921 aaagcccttt cggcgaatta agctgtttcc caataaggga ggaaatgagc caaccaccgc
     1981 agttaaacca tgttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnngtgcata ccggtcaaaa tacggggata tgttttttcc acctaaacag cctacgtttc
     2101 gagccgcaaa actttccaaa agtattatta ctacattctt tttgttgaat gggatgggcg
     2161 ctaaaaaatg tcgtaaagtt ggatagtatt cgctgtctga tttttcatcc ggcttaatca
     2221 ctttttcttt tagaccgtta agggcggatt gcacagaatt aaattcataa gccgctatat
     2281 ctctgtcaaa gatatttttt tggtcaatgg cttttacaaa agtgtaaaca ccattgcttg
     2341 ccatttgatt agcagcagtg ctactgctaa aggaggttag ataccaaaag ggtgggccat
     2401 aatacataaa ggagagcgca aaaaacatca agcaattcca tgcaatgaaa attggatttc
     2461 gttgctgggc tttatccagt gatttgaata gccttttatg cagatgaaga aacacaaaaa
     2521 caccgaaact gtatacaaga aggagtggta tcaccggata agatttccat atgagactaa
     2581 aaataatgga ataacgttga acaccttctg aaaccatcat atttaaacga gtgccgtatt
     2641 gtgtgtaata aaaaatgtca atggcattaa taaaaaatac aagnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt tcaacaagtg caaatggaag atagatacaa
     2761 aatagcaggg aggctgttac aaccgaatcc ataaatgttc ccaccaaaaa ggagtgaatc
     2821 aaattggaat cagtgagtga tacgagtgca ttggcatgaa tgaatgcaaa gcaaaaacgc
     2881 gcaatcaata aggtggcaag gcttaatgaa tatagtttga agctataaag taaaaaggat
     2941 tttgaaagca tttgagcaag gcaaaagggt taaaagaaag aatttctaaa attagtcaaa
     3001 attatggagc attttaattg ccacaaaaac aattagttag gttttatttt taaaaagatg
     3061 ttgcaacatc aattggttaa acatatattt gcattctcaa aaaaattatt aactaaaact
     3121 aaatttaaaa tgaaaaaatt aatgttaagt attgcaatta tcgggggagc tgttttaaac
     3181 atgtcggcaa aacctgaaac atctgcttgg gctgtggata aatcacactc gggtgtaaac
     3241 ttttcggtta cacacatgat gatggcggaa acagatggaa agtttaaggt ttttgatggt
     3301 tccatcattt caaccaatga agattttaca gatgctcaaa tcaattttac agtggatgtt
     3361 agcagcgtaa ataccgatga cgaaaaaaga gatgggcatt taaaatcaga tgattttttt
     3421 aatgctgaga agtatcctca gatgaagttc aaaggaacat cctttaaaaa agtagacgga
     3481 aaaaaataca ttttagaagg tgaactaacg attcgtgata taaccaagaa agtaaagttt
     3541 gatgtggtat atggcggaac tgcaaaaaat ccttggggaa tgacagtagc tggctttaag
     3601 gcaagtacaa ctataaaccg acttgattac aacttaaaat ggaacaaagc attagaagca
     3661 ggaggagttt tggttggtga agaggtaaca gtgaaagtga acatggaaat ggtaaaggga
     3721 aaataaggtg gattagtttt agtggagaag ggtgcacgaa ttttgtgtac ccttttttta
     3781 ttcataaaat cagttaattt agctttctat tagcaattta aaaagatgac aaaaacaatt
     3841 tcagaagcaa tcgtacaatc aaaatttcgc aatgagtacc ataaggcagg cgttgccctt
     3901 gcctacgcct acaatcaggt aaactcctta cagcataatt tttttaaaga gttttctatt
     3961 acgatgcaac aatttaatgt gttgcgcatt ttgcgtgggc aaagtccaaa cgtttgcaat
     4021 ttaattctga ttaaggaacg tatgatcgat aaaatgagtg atgtttcaag aattgtggaa
     4081 cgtttgcgtg tggctggcta tgtggagcgc tgcctgaatg aaaaaaacag gagagaagtt
     4141 gagattaaaa tttcaaaaag cggattaaaa t
//
LOCUS       DAORQT010000129         4154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3844_length_4154_cov_8.351061, whole genome shotgun sequence.
ACCESSION   DAORQT010000129 DAORQT010000000
VERSION     DAORQT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_4154_cov_8.351061"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="PK134_05500"
     CDS             complement(<1..1779)
                     /locus_tag="PK134_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRB38338.1"
                     /translation="MSDILHHECGVAMIRLLKPLDYYHKKYGTPFYALNKLYLLMEKQ
                     HNRGQDGAGVANIKFDAEPGTRYISRYRSNDKHAIQDIFSRIYNKFKADVNDDVSKLN
                     DTAWMKKHLAFSGEVWMGHLRYGTYGGNGIEACHPFLRQNNWMTRNLVVAGNFNMTNV
                     DELFENLIRIGQHPKEKADTVTVMENIGHYLDEENERLYYSFKEKGLSKQDISPLIAE
                     HLDVESILKQSSKKWDGGYVICGMMGHGDAFVLRDPAGIRPAYMYADNEVVVVASERP
                     AIQTAFNLSFSDITEIKPGHALIMKKNGNISMPCIKEPVERKSCSFERIYFSRGSDAE
                     IYQERKELGRLLCKSILPAVDNDLRNTVFSFIPNTAEVAFYGMMQGMEDYLREVKKKK
                     IKSLGKEISDEKLNEILSIKPRFEKIAIKDAKLRTFITNDDDRSDMVTHVYDITYGTI
                     RKGIDNLVVIDDSIVRGTTLKNSIIRMLDRLGPKKIIIVSSAPQIRYPDCYGIDMAKI
                     GDLCAFKAAIALLEENNMEHIIEETYMHCKQQLELGVNEMKNYVRDLYKPFSPEHLSA
                     KITSMLKPEDCKADVEIIFKKIEDMHI"
     gene            2223..3134
                     /locus_tag="PK134_05505"
     CDS             2223..3134
                     /locus_tag="PK134_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38339.1"
                     /translation="MNKIFAILLLTSGSCIGQNLVPNDGFEIHTSCPYVGNSFSTLSY
                     WFNPSLNLGWGSTPEYVDVPSNWAGYQFARNGVAYVGIVLWHNQLTFREYFSVQLISP
                     LVAGTSYHFEMYFNLANQFKYSCSNIGVYFSDTLYTSTINYDPLPFNPQINNINSNYP
                     DTINWSMVSGNYVAHGGENYIIIGNFQNDSNSYPILVDSNAFESGTYVFIDDVSLTVY
                     TGITEQEKIEVIKVYPTLFADKLYLSCEVNESCCVILYDETFRKIIQLPFTKNASLNT
                     GHLAKGMYFYEVRCGSSLCKKGKLVKD"
     gene            3302..3688
                     /locus_tag="PK134_05510"
     CDS             3302..3688
                     /locus_tag="PK134_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRB38340.1"
                     /translation="MKHEDVTRRIIASAMKVHSTLGNGFQEVIYQRAMELEMPFENLF
                     FKREMEMPVHYRNEIIGTRRVDFFVEEIVMVELKAIIKLEDVHLAQAMNYLEAYNMEI
                     GLLINFGSKSLEFKRVHNNKPILKSN"
     gene            3742..>4154
                     /locus_tag="PK134_05515"
     CDS             3742..>4154
                     /locus_tag="PK134_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38341.1"
                     /translation="MRKQICVIILMLTCRICDAQNLVPNGDFEQYSLCPDFPANLALA
                     LFWFNPSTNVQNVSGTSDYYNACANDFYPMNVPGSFTGYQVPHSDSAYAGILLFEHLG
                     VNYREYIEVQITSTLTSNTCYHFEMYISLGDIGKYT"
BASE COUNT         1272 a          758 c          767 g         1357 t
ORIGIN      
        1 aatgtgcatg tcttctattt tcttaaaaat tatttctaca tcagctttgc agtcttcggg
       61 ttttaacatt gatgtgattt ttgccgaaag atgctcaggc gagaaaggct tgtataaatc
      121 acgcacataa tttttcattt cgtttacacc caattcaagt tgctgcttgc aatgcatgta
      181 ggtttcttca ataatatgct ccatattatt ttcttcaagc aatgcaatgg ctgctttgaa
      241 tgcacacagg tcaccgattt ttgccatgtc aattccataa caatcggggt agcgtatctg
      301 tggtgctgac gaaacaatta taattttctt cggacctaaa cgatcgagca ttctgatgat
      361 agaatttttt aatgttgtgc cacgcacaat gctatcgtct ataaccacta aattgtcaat
      421 gccttttctg atggttccgt aagtaatatc ataaacgtgt gtcaccatat cgctgcggtc
      481 gtcatcgttg gtgatgaagg tgcgcagctt ggcatcttta atcgctattt tttcaaacct
      541 gggtttaatg gaaagaattt cattgagctt ttcgtcagaa atttcttttc ctaaagattt
      601 tatttttttc ttttttacct cacgcaaata gtcttccatt ccctgcatca tgccatagaa
      661 ggcaacttct gccgtattgg gtataaagga gaaaacagtg tttcttaaat cgttgtcaac
      721 agcaggcaga atacttttgc aaagcaggcg tcctaattct tttctttcct gataaatttc
      781 tgcatcgctg cctcttgaaa aatatattct ttcaaacgag catgatttgc gttcaacagg
      841 ttcttttata caaggcattg aaatgttccc gttttttttc attatcagtg catgaccggg
      901 ttttatttcg gtaatgtcgc tgaacgataa attaaatgct gtttgtatgg caggacgctc
      961 gctggctaca actaccactt cattatcggc atacatatat gcaggacgaa tgcctgcagg
     1021 atcgcgcaac acaaaagcat caccatgacc catcatgccg cagataacat agccgccatc
     1081 ccattttttt gaagactgtt ttagaatgct ttccacatca agatgctcgg caatgagcgg
     1141 tgagatatct tgtttagaaa gccctttctc tttaaatgaa tagtataatc tttcgttttc
     1201 ttcgtcaagg tagtgaccga tgttttccat tacggtaacc gtgtcggctt tttcttttgg
     1261 atgctgacca atacgaatca gattttcaaa caactcatca acattggtca tattaaaatt
     1321 tccggccaca actaagttgc gtgtcatcca gttgttttgt cgcagaaaag gatggcaggc
     1381 ttcaatgcca ttgccaccat aggtgccata gcgcaaatgc cccatccaca cttcgccact
     1441 gaaggctaga tgttttttca tccatgcagt atcattcagc ttcgatacat catcattaac
     1501 atcggcttta aacttattgt atattctgga aaaaatatcc tgaatagcat gtttatcgtt
     1561 ggaacggtag cggctgatgt agcgtgttcc gggttcggca tcaaatttta tgttggcaac
     1621 accggctcca tcctgtccac ggttgtgttg cttctccatc agcagataga gtttgtttaa
     1681 tgcataaaaa ggagtaccgt attttttatg gtaatagtca aggggcttta gcagccttat
     1741 catggctacg ccacattcgt gatgaaggat atctgacatg aagctacaaa gatatagcaa
     1801 aaaggagtac acgacaatag catttcataa aatatcagct gtgccccgta aaaagcaata
     1861 cagagtttat gtttaactat tccggggagg cgtggtgcta ggaaataatt aatcaatagc
     1921 gccctgaata atttcatctt ttaggtaacg ctgcccctct gacagtgtga tttaaacaac
     1981 attttatttt cttcatccac aattttcatc agtgacattt tagaaaattt taacattcta
     2041 aacttttcaa ctttctaaac cttcttttga caattgcgat aatccgcaca atgagttgcg
     2101 aatattttgt atatttgttt cataatgaat aaacttgtga attgtgagtg ttgataatat
     2161 aaatgttatc agccatttta acgctccgaa caaactgtac tgctaattca aaacgacata
     2221 ccgtgaataa aatctttgcc atattattac taaccagtgg aagttgcatc gggcaaaacc
     2281 ttgttcccaa tgacggcttt gaaattcaca catcttgccc atatgttgga aactcattca
     2341 gtacgttatc ctattggttc aacccatcct taaatcttgg ttggggttct acaccagagt
     2401 atgttgacgt cccatcaaat tgggctggtt accaatttgc acgaaatggt gtagcatatg
     2461 ttggaatagt gctttggcat aatcaactta ctttcagaga atattttagt gtgcagttaa
     2521 tatcaccatt ggttgcaggc acttcttatc attttgaaat gtatttcaat ttggcaaacc
     2581 aattcaaata ttcttgcagt aacataggag tatatttttc agatacttta tataccagca
     2641 ctatcaacta tgacccctta ccttttaatc cccaaataaa taatataaac agcaattatc
     2701 ccgatacaat taattggtca atggttagcg gcaattatgt tgctcatgga ggagaaaatt
     2761 atattatcat tggtaatttt caaaatgact ctaacagtta tccaatatta gtagattcca
     2821 acgcatttga atcaggcact tatgttttta ttgatgatgt ttccttaact gtctatacag
     2881 gaatcactga gcaagagaaa atagaggtta ttaaagtata tccaactctg tttgcagata
     2941 aattatattt atcatgcgaa gtcaacgaaa gttgttgtgt aatactttat gacgaaacct
     3001 ttagaaaaat tattcaactc ccattcacga aaaatgcttc actaaacaca gggcatcttg
     3061 caaagggaat gtatttttac gaagtacggt gtgggagtag tttatgcaag aaaggaaagt
     3121 tggtgaaaga ttgaaattat acagcttcgt ggacacagca aaacatgcgc ataacagtaa
     3181 gattaaaaga aataaaaaaa agaaggtccg atagttatcg gactttgaga ggtaaacaaa
     3241 attgtctgaa ccatgattaa aaggattaga agattacttg attttaaaca aaagatttga
     3301 aatgaagcat gaggatgtta ccaggcgaat aattgcctcg gcaatgaaag ttcattccac
     3361 gctgggaaat ggtttccagg aagtaattta tcaaagagcg atggagttgg aaatgccatt
     3421 tgaaaatttg ttttttaaaa gagaaatgga aatgcctgta cattaccgta atgaaataat
     3481 tggtacaaga agagtagatt tctttgttga agaaattgtg atggttgagt tgaaagcaat
     3541 tattaaattg gaagatgtgc atttggcaca ggcaatgaat tatcttgagg catataacat
     3601 ggaaataggc ttattaataa actttggaag taaaagttta gagtttaaaa gagttcacaa
     3661 taataaaccc atcctaaaat caaattaatc aggcaaatca tggttcagac aaacagtgtt
     3721 gctaatacaa acagttcgac aatgagaaaa caaatctgcg ttatcatttt gatgttgact
     3781 tgcagaattt gtgatgcaca gaatcttgta cctaatggag attttgagca gtattcgtta
     3841 tgtccagact ttccggctaa ccttgctttg gccttatttt ggttcaatcc ttcaaccaat
     3901 gtccaaaatg tttctggaac ctctgattac tataatgcat gcgccaacga cttttacccg
     3961 atgaatgtac ctggtagttt cactggttat caggttcctc acagtgattc tgcttacgca
     4021 ggaatcttat tatttgaaca tttaggagtt aattatcgcg aatatatcga agttcaaatt
     4081 acttcaactt taacaagtaa tacctgttat cattttgaaa tgtatatcag tctaggtgat
     4141 attggtaaat atac
//
LOCUS       DAORQT010000130         4151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3852_length_4151_cov_7.796387, whole genome shotgun sequence.
ACCESSION   DAORQT010000130 DAORQT010000000
VERSION     DAORQT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3852_length_4151_cov_7.796387"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1371)
                     /locus_tag="PK134_05520"
     CDS             complement(172..1371)
                     /locus_tag="PK134_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRB38342.1"
                     /translation="MKNLLTLLLLLSILNLRADYWTQKANFPGIAREAPFSFSIGNSG
                     FVGGGRDAFSVFHNDLWEYYPTTDSWTQKANFGGTPRYAAIGFAIGNKGYVGVGFDFL
                     GVLKQDFWEYNPSANVWTQKANFGGGNRYLAIGFSINNFGYCGTGYSTITTISNDFWE
                     YNPINDTWLQKTNMTGYYRAGANSFVIGNKGYVVGGFDTTGSVFYSDLWEFNQSLNSW
                     SQKSNLPSGGRCDASSFSICGKGYLGVGLDNGIVLNDLWQYNSVSNTWVSKANFPGLN
                     RYMAAFFSIGDKGYIGLGANGLFQNDFYEYTPDSLCATNIKEENNFNKSMFVFPNPIN
                     NELNISSNDKVQKEIILYDVSSKKLLQQKFTNSISLNTEGLAKGIYIYEVRDKNGSFK
                     KGKVVKD"
     gene            complement(1872..4133)
                     /locus_tag="PK134_05525"
     CDS             complement(1872..4133)
                     /locus_tag="PK134_05525"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRB38343.1"
                     /translation="MPVNKEALDYHASGRPGKIEVIPSKPYSSQRDLSLAYSPGVAEP
                     CLEIEKDIDNVYKYTAKGNLVAVISNGTAVLGLGNIGPEASKPVMEGKGLLFKIFADI
                     DVFDIEINQTNVDDFVKTVKAISPTFGGINLEDIKAPECFEIESRLKNELNIPVMHDD
                     QHGTAIISGAALINALDIVNKKIDLVKIVVNGAGASAISCTKLFVSLGAQKKNITMLD
                     STGVIRKDREGLDANKREFATDRNINTLEEAMRGADVFIGLSKGNILSADMLKSMADK
                     PVVFAMANPNPEITYPDAKSAREDVIMATGRSDFPNQVNNVIGFPFIFRGALDVRATK
                     INEEMKLAAVHAIAQLARQPVPEMVNLAYNTKNLQYGPEYIIPKPLDPRLIYTVAPAV
                     ARAAMETGVAQVSITNWEHYVEDLKKRLNLDNKLIRAIVTRAKQNPQRIVFAEADNYK
                     ILKAAQQVKEEGIARPILLGNRERITQIVTENHIDITDIPIVDPRVDTEHLNTFGELF
                     FEKAKRRGFSVYEAKKVMRERNYYGAMMVETGQADALISGLTRNYADTIRPALRIIGV
                     QEHGTRVAGMYIMMTKKGPLFFADTTINVNPTSEDLVEITLLTAKAVQQFNIKPRIAL
                     LSFSNFGTSNDPSAIRIRKAVEILHDKFPGMIVDGEMQANFALNMQMLHDSYPFCELI
                     NGGVNTLIFPNLDAGNIAYKLLQSMEAAEAIGPVLLGMKKPVHILQLGSSVRDIVNMV
                     TIAVIDAQSRKQQ"
BASE COUNT         1193 a          811 c          812 g         1335 t
ORIGIN      
        1 gaaaattttt taacactcat ttgtaggacc cccaaatatc agatattttg caaggggcta
       61 cacatcatgt caggtgaaat attgatttta acgctactca actactgcaa ttaaaatttc
      121 aatcactaat atttacaggg gaatctttct gttatcagcc gtctctctct ctcaatcctt
      181 cacaactttt cctttcttga acgaaccatt tttgtcgcgc acttcgtaaa tataaattcc
      241 ttttgcaagt ccttcagtat ttaatgaaat cgagtttgta aatttctgct gcaataattt
      301 ctttgaagaa acatcatata gaataatttc cttttgcacc ttgtcattag aactaatatt
      361 caattcatta ttaattggat ttgggaaaac aaacattgat ttattaaaat tattttcttc
      421 tttaatattt gttgcacata aactgtcagg cgtatattca tagaaatcat tttgaaaaag
      481 tccatttgcc cccaatccaa tatatccctt gtctccaatt gaaaaaaaag ctgccatata
      541 tctatttaaa cctggaaaat ttgcttttga gacccatgtg ttgctaacag agttatattg
      601 ccataaatca tttaatacga ttccattgtc caacccaact cctaaatatc cctttccaca
      661 aattgaaaat gaagaagcat cacaacgtcc accagaaggt aaattagatt tttggctcca
      721 actatttaaa gattgattaa actcccataa gtcagaatag aatactgaac ctgttgtatc
      781 aaagccacca actacatatc ctttattgcc aataacaaac gaattagcac ctgctcgata
      841 atatcctgtc atattcgttt tttgcaacca tgtatcatta ataggattat attcccaaaa
      901 atcattgctt atggtggtga ttgtgctata acctgttcca caatagccaa agttgtttat
      961 tgaaaaacca attgcaaggt atctattccc tcctccaaaa tttgcctttt gtgtccatac
     1021 atttgctgat ggattatact cccagaaatc ttgcttcaaa acgccaagaa aatcaaatcc
     1081 gactcccaca tatcctttgt tgccaatagc aaatccaata gccgcatatc ttggtgtgcc
     1141 gccaaaattt gctttttgtg tccatgaatc agtcgttggg taatactccc ataaatcatt
     1201 gtgaaacaca gaaaaagcat ctcttccacc gccaacaaag ccgctgtttc caatggaaaa
     1261 agaaaaaggt gcttctcttg caattccagg aaaatttgct ttttgtgtcc aataatctgc
     1321 ccttaaattt agaatggata atagtaataa aagagtcaaa agattcttca tagttgtaat
     1381 atttgaatat atactaacat ttgttctgtt tattaaaaag attaaaaacg tcagtctgtc
     1441 aaatgaatat cgttaaaata gctatctgaa ttaaatctat acatcagttc aaaagtaaag
     1501 agaaaagtaa ttagattgtt gagaattaat aagtgaatag agagaagtga tgaagtaatt
     1561 tgataattat gaatgcagag ttcagaattc agtattatca attagccacg gctgatatgg
     1621 gtgagaaatg taaaatgtaa aataataaat ctaaaatgta aaagtgaaaa gtgatgagag
     1681 caactaatac acagcaagaa aatgttttac acataaacaa ttagccctga tgatcccgat
     1741 agcgatcggg aggaggtttt gtgcgtgagc ggtgccatgc accgaccccg ataattatcg
     1801 gagaaactat tgtaaaagtt actgcagttt gtgttctaaa aaattatcta ctcaaacaag
     1861 acgtttaacc actactgctg ttttctgctt tgcgcatcaa taacagcaat tgttaccatg
     1921 tttactatgt cgcggacgct gctgccgagt tgcagaatgt gtaccggctt tttcatgcca
     1981 agaagtacag gaccaatagc ctctgctgcc tccatagatt gcagaagttt gtaggcaatg
     2041 tttccggcat cgaggtttgg aaagatgagt gtgttgacac caccgttaat gagctcacaa
     2101 aacggatagc tgtcatgcag catctgcatg ttgagtgcaa agtttgcctg catttcgcca
     2161 tctactatca tacccggaaa tttgtcgtgt aatatttcta cagcttttct gatgcgaata
     2221 gctgaaggat cgtttgaagt gccgaagttg gagaatgaca agagtgcaat gcgcggcttt
     2281 atgttgaact gctgcacggc tttggctgtg agcaaggtta tttccaccag atcttctgat
     2341 gtaggattta cattgatggt ggtgtcggca aaaaacaaag ggcctttttt tgtcatcata
     2401 atgtacatac ctgcaacacg agttccatgt tcctgcacac cgatgattcg taatgccgga
     2461 cggatggtgt cggcatagtt acgtgtgagg cctgagatga gtgcatcggc ctgtccggtt
     2521 tctaccatca tggcaccgta gtagtttctt tcgcgcatta cttttttagc ttcgtaaacg
     2581 gagaagcctc tgcgttttgc tttttcgaaa aagagttcgc caaaggtgtt gaggtgttcg
     2641 gtgtcaacac gagggtcaac gatgggaatg tctgttatat caatgtggtt ttctgtgacg
     2701 atttgtgtta ttcgctcgcg gttgccgagc aaaattggtc gtgcaattcc ttcttctttg
     2761 acctgttgtg cagctttaag aattttgtag ttgtcggctt ctgcaaagac tatgcgttgc
     2821 ggattttgtt tggctcgtgt tacaattgca cgaatgagtt tattatcgag gttgagacgt
     2881 tttttgaggt cttctacata gtgttcccag ttggtgatgc tgacttgtgc cacacctgtt
     2941 tccatagcag cacgtgcaac ggcaggtgca acggtataaa tcaatcgtgg gtcaagtggt
     3001 ttggggataa tgtattcggg gccgtactgc agattttttg tgttgtaagc aaggtttacc
     3061 atttcgggaa ctggctgtcg tgccaactgc gcaattgcat gcaccgctgc cagtttcatc
     3121 tcttcgttga tttttgttgc acgcacatct aaggctcctc tgaaaataaa tggaaaacca
     3181 attacgttat ttacctgatt gggaaagtcg ctgcggcctg ttgccatgat tacatcttcg
     3241 cgtgcactct tggcatcggg ataagtaatt tccggattgg gattggccat agcaaaaaca
     3301 acaggtttgt cggccataga tttgagcatg tcggcactga ggatatttcc tttgctgaga
     3361 ccaataaata catcggcacc gcgcatggct tcttcaagcg tgtttatgtt tctatcagtt
     3421 gcaaattctc ttttgttggc atctaaccct tcgcggtctt ttctgataac accggtactg
     3481 tcgagcatgg tgatgttttt tttctgtgca ccaagcgaaa caaaaagttt ggtacatgaa
     3541 attgcagaag cacctgcacc attgacaaca atttttacga gatcaatttt tttgtttaca
     3601 atatctaatg cattgatgag tgctgcaccg ctgatgatgg cagtgccatg ctgatcgtca
     3661 tgcattacag gaatgttcaa ctcattcttt aacctacttt caatttcaaa acattctggt
     3721 gctttaatgt cttcgaggtt tatgccaccg aaagttggtg aaatggcttt tactgttttt
     3781 acaaaatcgt caacgtttgt ttgattgatt tcgatatcga atacatcaat gtctgcaaat
     3841 attttaaaca gcagtccctt accttccatg acaggttttg atgcttcggg gccaatgttg
     3901 ccaagaccaa gtacggcagt gccgttagaa attacggcaa caagatttcc tttggcagtg
     3961 tatttataaa cgttgtcaat gtctttttca atttcaagac aaggctctgc aacaccgggc
     4021 gaataggcta atgataaatc gcgctgtgag ctataaggct tactgggtat cacttcaatt
     4081 ttgcccggac gacctgaggc atgataatcg agtgcttctt tattaactgg cataattatt
     4141 ttttttacaa a
//
LOCUS       DAORQT010000131         4127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3885_length_4127_cov_10.018910, whole genome shotgun sequence.
ACCESSION   DAORQT010000131 DAORQT010000000
VERSION     DAORQT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_4127_cov_10.018910"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PK134_05530"
     CDS             <1..1337
                     /locus_tag="PK134_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRB38344.1"
                     /translation="ETYKLYTKVNLVNYSIDKLPALNGFWSQELKMPQQLELTTENYN
                     GEMYRVGIVKKVLLFPQQSGTLTIEPMEGSCIARIQTQRKRSNNPFDIFNDPFFNDPF
                     FGSAQDVKVNVKSLPVKITVKDLPQENDNAFKGAIGSFSLQATTDNDKVKANDAINLK
                     IKISGKGNLKLIDAPEVEVPTDFEKYDPKVSDNTNISESGVSGSKTFEYLLIPRHEGE
                     YTIDPIKFTYFDLDKKQYEQLQSSAFKITVKGISGDASAAQSVATSQNKTDIKLLGKD
                     ISFIKTGKLTTKPATTFYLSGLYYLLVAIPFAALILLILYIRKRNRLAGDAEYMRSSR
                     ATKMAHKRLSIAKKMIDAKKDDAFYEEISKAIYGYASDKLNINFSEMTKENLRQKLIN
                     RNIPDALTERIIATIDLCELARYAPLTQNNNTQSIYTEAAAIITELENYLNK"
     gene            1341..2102
                     /locus_tag="PK134_05535"
     CDS             1341..2102
                     /locus_tag="PK134_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38345.1"
                     /translation="MKARYIFILLCLLLSQNIFADSKHDIVFNEANNLYLKQSYFQAI
                     EKYEQLVADNTISFEVYYNLGNAYYKTGNFTKAILNYERAKKIKPDDADVNLNLAIAN
                     QKTIDKIEPTPKVFYNQWWDNYIASSTADSSAVIMLFFLWLFIAAIAIMIIIKSASLK
                     KLCFIISLILACCTLFFFITTQTRQNINDSSVEALIISQSAYIKSAPDDKGTNLFQLH
                     EGTKINIVDELSSWKKIRIPNGNIGWIKSKDLETI"
     gene            complement(2103..3998)
                     /gene="asnB"
                     /locus_tag="PK134_05540"
     CDS             complement(2103..3998)
                     /gene="asnB"
                     /locus_tag="PK134_05540"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB38346.1"
                     /translation="MCGIAGSFSVSNFFSEQDFRKASAAIKHRGPDGDGFYSDNFVRL
                     AHRRLSIIDLDNRASQPMYSSCMRYVMVYNGEVYNYVELRKEIESEYKDSQLSDFKTT
                     SDTEVILRLFEIKGVDFVKDLNGMFAIAIYDTQANKLFLFRDRFGIKPLYYYHDSSGN
                     FCFASELKAILAIDSIERKVNSQAVAYFLHTGFVPAPLTMYEKVFKLESGNFLTIDRN
                     GLEIHPFVMNRKCSQREIEHDEKKAIQNIEKLLIQSVKIQLRSDVPYGVFLSGGVDSS
                     LLTAIAARNVDEKLNTFSIGFAEQSHNESEHARVIAKHIGTNHHEFVVSVKDAIDLIE
                     TFFEVYDEPYTDTSGIPTMLVSKLASKHVKVVLSGEGGDELFFGYGTHNWAKRLNAFP
                     FSVFRSPIRTILTCGNSRSKRVAQLLDFNEHSDFLPEHIYSQEQYLFSNKEIVELVSD
                     ELRKETLKNIAQRNEELEKLFNINDSCLPEERQANYELKFPFQDDLLTKVDRATMFHS
                     IEARVPFLDNNFAEYVFSVSSDIKIKNGINKYPLKKILANYVPEELFMRPKKGFSIPL
                     AKWLKADWKYLLNRYLDEKTIRHAGLVKFEVVKKLIQRFLSGEDYLYNRIWCLIVLHR
                     WYERQQW"
BASE COUNT         1472 a          752 c          629 g         1274 t
ORIGIN      
        1 tagagacata taaattatat actaaagtaa atttggttaa ctatagtatt gataaactac
       61 ctgcactaaa tgggttctgg tcacaagaat taaaaatgcc acagcaatta gaactcacta
      121 cagaaaatta caatggtgag atgtatagag ttggaattgt taaaaaagta ttgttgtttc
      181 cacagcaatc aggcacatta actatagagc caatggaagg cagttgcatt gcccgtattc
      241 aaacacagcg caaacgttcc aataacccat tcgacatttt taacgaccca ttttttaacg
      301 acccgttttt cggatcggca caagatgtaa aagttaatgt taaaagctta cctgtaaaaa
      361 ttacagtaaa agatcttcct caggaaaatg acaatgcatt taaaggggcg attggatcat
      421 ttagtttgca ggcaacgact gataatgata aagttaaagc taatgatgct attaatttaa
      481 aaataaaaat atccggcaaa ggaaatttaa aacttattga tgcacctgaa gttgaagtgc
      541 caactgattt tgaaaagtat gatccaaaag tcagcgataa tacaaatatt tcagaaagtg
      601 gtgtttcggg cagcaaaact tttgaatatt tattgatacc acgtcatgaa ggtgaatata
      661 caattgaccc aataaaattc acctatttcg atcttgacaa gaaacagtat gaacagttac
      721 aatcatcagc atttaaaatt acagttaaag gaatttcggg agatgcaagt gctgcacaat
      781 cagtagcgac atctcaaaac aagacagaca ttaagcttct tggcaaagac atttcattca
      841 ttaaaaccgg aaaattaaca acaaaaccgg caacaacatt ttatctgagt ggtttatact
      901 atttattggt tgcaatccca tttgcagcac ttattttatt gatattgtat atcagaaaaa
      961 gaaatagact tgccggtgat gcagaataca tgcgaagtag cagagctaca aaaatggcac
     1021 ataaacgatt gtcaattgct aaaaagatga ttgatgcaaa gaaagatgac gctttctacg
     1081 aagaaatatc caaagctata tatggatatg cttctgacaa actaaatatt aatttttctg
     1141 agatgacaaa agaaaatcta aggcaaaaat taatcaacag aaatattcct gatgctttaa
     1201 ctgaaagaat cattgcaaca attgacttgt gcgaacttgc cagatacgct ccactcacac
     1261 aaaacaataa cacacaaagc atttacactg aagctgctgc catcattaca gaactcgaaa
     1321 actatttaaa caaataaaat atgaaagcaa gatatatatt catcttacta tgcctgttgc
     1381 tttcgcagaa tatttttgcc gactcaaaac atgatatagt ctttaatgaa gctaacaacc
     1441 tttatttaaa acaaagttat tttcaggcaa ttgaaaaata tgaacaactc gttgcagaca
     1501 ataccatttc atttgaagtt tattataatc tgggtaatgc atattacaag acaggcaact
     1561 tcacaaaagc aattttaaat tacgaaagag caaaaaaaat taaacctgac gatgctgatg
     1621 tcaacctcaa tttagcgata gctaatcaaa aaacaattga caaaattgaa ccaacaccaa
     1681 aagtatttta caatcagtgg tgggataatt atatagcctc ctccacggca gactcatcgg
     1741 ctgtcattat gttatttttt ctttggttgt ttattgcagc aatagccatc atgattataa
     1801 ttaaaagtgc ttcattgaaa aaattatgtt ttatcatcag tttaattctg gcttgctgca
     1861 ctttgttttt ctttatcaca acacaaacac gccaaaacat aaacgactca tctgtggaag
     1921 cattaatcat aagccaaagc gcttatatca aaagcgcgcc tgatgataaa ggcaccaact
     1981 tgttccaatt acatgaagga acgaaaatta atattgttga tgaattgagt tcttggaaaa
     2041 aaataagaat ccccaatggg aatattggat ggattaagtc taaagatctg gaaacaattt
     2101 aatcaccatt gctgtcgctc ataccagcga tgaagtacga ttaagcacca gatacgatta
     2161 tataaatagt cttctccgga aagaaatcgt tgaattaact tttttacaac ttcaaatttc
     2221 actaatcctg catgtcgaat tgttttttca tcaagataac gattaagcaa atatttccaa
     2281 tcagctttta accattttgc aagaggaata gaaaatcctt ttttgggtcg cataaataat
     2341 tcttcaggaa cataattggc aagaattttt tttagtggat atttgtttat tccattcttg
     2401 attttaatgt cactggaaac tgagaaaaca tattcagcaa aattattgtc taaaaaaggc
     2461 acacgtgctt ctatagaatg aaacatagta gctctatcaa ctttggttag caaatcgtcc
     2521 tgaaatggaa attttagctc ataatttgcc tgtctttcct ctggcaaaca actgtcatta
     2581 atattaaaca acttttccaa ttcttcattt ctttgtgcaa tattcttcaa ggtctctttc
     2641 cttaattcat cagaaaccaa ttccactatt tctttgttac tgaacaagta ttgttcttgt
     2701 gaataaatgt gttcaggcaa aaaatcggaa tgctcattaa aatccaataa ttgtgcaaca
     2761 cgtttactgc gtgagttacc acaagtaaga atagttcgga taggacttct gaaaacgcta
     2821 aaagggaaag catttaatcg cttagcccaa ttatgggtac catagccgaa aaacaattca
     2881 tcaccgcctt ctcctgacaa cactaccttt acatgctttg aagcaagttt tgagactaac
     2941 attgtaggaa taccagaggt atcagtataa ggttcatcat aaacttcaaa aaaagtttca
     3001 attaaatcaa ttgcatcttt aacagaaaca acaaactcat gatgatttgt tccaatgtgt
     3061 tttgcaatca ctcttgcatg ttctgattca ttatggcttt gttcagcaaa accaattgaa
     3121 aatgtattca gtttttcatc aacgtttctt gctgctattg cagtaagcaa acttgaatca
     3181 acaccaccac ttaaaaagac tccataagga acatcgctgc gcaattgaat ctttaccgat
     3241 tgtatcagaa gtttttcaat gttttgaatt gccttttttt catcgtgttc aatttctcgt
     3301 tgtgaacatt ttctattcat cacaaacgga tgaatctcca aaccgtttct gtcaatagtc
     3361 aagaaatttc cgctttcaag cttaaatact ttctcataca tcgttaatgg tgcaggaaca
     3421 aatccagtat gaagaaaata tgctacagcc tgactattta cttttctttc aatactatca
     3481 attgcaagaa ttgctttaag ttcagatgca aaacaaaaat ttcctgatga atcatgatag
     3541 taatataaag gttttatccc aaatctatct ctaaacaaaa ataatttgtt ggcttgtgtg
     3601 tcataaatgg caattgcaaa catgccattc aaatctttaa caaaatcaac accttttatc
     3661 tcaaacaatc ttaaaataac ttctgtgtca cttgtagttt taaaatcaga caattgactg
     3721 tctttatatt cagactcaat ctctttacgt aactctacat aattatagac ctccccatta
     3781 taaaccatca cataacgcat gcaggaagaa tacataggct gtgatgctct attgtcgaga
     3841 tcaattattg ataacctgcg atgtgccaat cggacaaaat tatcactgta aaatccatct
     3901 ccatctggcc cacgatgctt aatggcagca gatgccttcc tgaaatcctg ctcagaaaaa
     3961 aaattgctta ccgaaaaact acctgctatt ccgcacatca gctgataaat atttttgcta
     4021 ttgccttata aaaatgcata tccgtatatg ttgaagggta cttcagaacc ttcattaatt
     4081 ctgttaatcc ctttcgggta taattggatg acagataaac attactt
//
LOCUS       DAORQT010000132         4114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3913_length_4114_cov_5.979552, whole genome shotgun sequence.
ACCESSION   DAORQT010000132 DAORQT010000000
VERSION     DAORQT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3913_length_4114_cov_5.979552"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK134_05545"
     CDS             <1..205
                     /locus_tag="PK134_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRB38347.1"
                     /translation="SHISGDANMVPTLTQIAFDLQYDGVMVEVHLNPVKALSDAMQQI
                     TPEDFSRLRKQIIVRQPTAKDPV"
     gene            232..513
                     /locus_tag="PK134_05550"
     CDS             232..513
                     /locus_tag="PK134_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRB38348.1"
                     /translation="MDELDSEIIEKIAGRMEVARKIGQYKDQNNMAILQLDRFKEILR
                     TRTETGLRNQLTPDFILRLYSLIHEESIAQQDKQMTKPELRASSKAEKH"
     gene            complement(522..671)
                     /locus_tag="PK134_05555"
     CDS             complement(522..671)
                     /locus_tag="PK134_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38349.1"
                     /translation="MSATNANSAVEYSYHKAHIFFSFDYYNPVKNQSTPVFYLGSGRN
                     YYQQN"
     gene            614..1588
                     /gene="aroB"
                     /locus_tag="PK134_05560"
     CDS             614..1588
                     /gene="aroB"
                     /locus_tag="PK134_05560"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRB38350.1"
                     /translation="MYVLCDTNTQQHCLHLLQTLFPQTKSASLYCIQQGESFKTIETV
                     TGFWKFLLQQQADKHTVIINLGGGVITDLGGFAASLFKRGVDFIHVPTTLLAMVDAAI
                     GGKNGVNIDGYKNQSGSIVLPRYVYVDTDFLESLSQDEKVNGYAEMIKHALVADQEFY
                     KVLSEQCSFEKLQSIGKAIAIKLSIVAIDPFEKKERKLLNFGHTIAHAIESLYYSQQQ
                     DISHGKAVAAGIIVESYISNKMGFISDAELKQITDFIQSLFKFDEKDIVVEKLLPFMM
                     ADKKNKQGQIRFTLLESIGKGIVDIAVPIAMIEEALVWFSKQDSGYGV"
     gene            1578..3041
                     /locus_tag="PK134_05565"
     CDS             1578..3041
                     /locus_tag="PK134_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38351.1"
                     /translation="MAYRISKTDRSVVGEIVVPVSKSEAGRVAIINALRGIKQNSDCH
                     ASDTVKLQELLFSEEQILDAADGGTTARFLMAYCVVKNKTAVITGSPRLRQRPMSDSV
                     DLLRQIGAEISYLEEEGFLPIRVRAAKLNFPEVVQTSAEKSSQHISALMMIAPLFGKD
                     FTIELNGISSSLPYIELTAYLMHQAGVNVLITENRIQINGRYNNSILSAGGDWSAASY
                     FFETVALADDADITIKNLNIQSKQGDKIIVEMIKPFGVDTYVEKDKIILRKQKKMVPD
                     YFTQSFLHFPDIVLSMATVCGGQNCKADLLHLHNLIIKESDRVSAFQREAYKLNIKTD
                     FCGGSKLKILEESNLKPSKRILKTYNDHRLVMAFAPLALKTGELFLDDISGVSKSFPD
                     YFEQMKSLGFNIEEVFLIISCKQRSMDLHPTRIYIPEPTQACSCLSELNFHYVLRMRH
                     YDVVLHHLHQHFCLLHKQHLCHHQQQHHVAAFLYVSF"
BASE COUNT         1322 a          687 c          841 g         1264 t
ORIGIN      
        1 aagtcatatt agcggtgatg ctaatatggt gccaacactg acacaaattg cattcgattt
       61 acagtatgat ggtgtgatgg tagaagtgca tttaaatcct gtaaaggcat tgagtgatgc
      121 aatgcagcaa atcacacctg aagattttag tcgtttaaga aagcagatta ttgtaagaca
      181 acctacggca aaggatcctg tttaagtaag ctgcacgatt tacgcgatct gattgatgaa
      241 ttggatagtg aaatcattga aaagatagcg ggtagaatgg aagtggcacg aaaaataggg
      301 cagtataagg atcagaataa tatggctatt ctgcagttag atcgttttaa ggaaattctc
      361 agaacaagaa ctgaaacagg gttacgaaac caactcacac cagattttat tttaagatta
      421 tatagcctga tacatgaaga gtcaattgcg caacaggata agcagatgac aaaaccggaa
      481 ctcagagcct catcaaaagc agaaaaacat tagaggggtg attaattttg ctgatagtag
      541 ttacgtcctg acccaaggta gaaaacagga gttgattgat tttttacagg attgtaataa
      601 tcaaaagaga aaaatatatg tgctttgtga tacgaatact caacagcact gtttgcattt
      661 gttgcagaca ttgtttccgc aaacaaaatc tgcttcttta tattgcatac aacaaggaga
      721 atcttttaaa acaattgaaa cggtaacggg attctggaag tttcttttac aacaacaggc
      781 tgataagcat actgtgatta taaatctggg tggaggtgta attacggatt taggaggctt
      841 tgcggcttct ctgtttaaaa gaggagttga ttttattcat gttcctacaa ccttgctggc
      901 aatggttgat gctgctattg gaggtaagaa tggtgtaaat attgatggct ataaaaatca
      961 aagtgggagt attgttttgc caagatatgt ttatgtagat acagattttt tagagtcgct
     1021 ttcacaagat gaaaaagtca atggctatgc agaaatgata aaacatgcac ttgttgccga
     1081 tcaagaattt tataaagtac tttcagagca atgcagtttt gaaaagttac aaagcattgg
     1141 aaaggctatt gcaataaaac taagtattgt tgcaatagat ccatttgaaa agaaggaacg
     1201 taagttgctc aactttggcc acactatagc tcatgcaata gagtcgttat attattcgca
     1261 acaacaagat atttcacatg gtaaggcagt tgctgccggg ataattgttg aatcttacat
     1321 ctctaacaaa atgggcttta tttcagatgc tgagctaaag caaatcactg actttattca
     1381 atcactgttt aagtttgatg aaaaagatat tgttgtagaa aaattgttgc catttatgat
     1441 ggctgataag aaaaacaaac aagggcaaat acgtttcaca ttgcttgagt ctattggtaa
     1501 aggtattgtt gatatcgctg ttccgattgc aatgattgaa gaggcattgg tttggttttc
     1561 taaacaggat tcaggttatg gcgtatagaa tttcaaaaac tgacagaagt gttgtaggag
     1621 aaattgttgt tcctgtttca aagagtgaag cagggcgagt tgctatcatt aatgctctta
     1681 gaggaattaa acagaatagt gattgtcatg catcagatac agttaagttg caagaactgc
     1741 ttttttcaga agaacagata ttagatgcag cagatggcgg aactacagcc cggtttctta
     1801 tggcttactg tgttgttaag aataaaaccg ctgtaattac aggaagtcct cgacttcggc
     1861 aacgcccaat gagtgattct gttgatttgc tcaggcagat aggagcagag atttcttatt
     1921 tagaagaaga aggttttctt ccgatcagag ttcgggcggc aaaactgaac tttcctgaag
     1981 tagttcagac atcagcagag aagtcttctc agcacatcag tgccttgatg atgattgctc
     2041 ctttgtttgg taaagatttt actattgaac tgaatggcat ttcaagttca ttgccatata
     2101 ttgaacttac tgcgtatttg atgcatcagg caggcgtaaa tgtgctgata acagagaatc
     2161 gaattcagat aaatggaaga tataacaata gcatactttc tgccggtggt gactggagcg
     2221 ctgcctcata tttttttgaa actgttgcat tggctgatga tgcagacatt acaattaaaa
     2281 atctgaatat tcagagtaag caaggtgata aaattattgt cgaaatgata aagccatttg
     2341 gcgtagatac ttatgttgag aaggataaaa ttattctaag gaaacaaaag aaaatggtgc
     2401 ctgattattt tacacagagt tttttacatt ttcccgatat tgtattaagt atggcaactg
     2461 tttgtggtgg acaaaattgc aaagctgatt tactacatct acataattta attattaaag
     2521 aatctgaccg tgtttctgcc tttcaaagag aggcatataa gttaaacatc aaaacagatt
     2581 tctgtggtgg ttcaaaattg aaaatccttg aagaatcaaa cctcaaacct tcaaagcgta
     2641 ttttaaaaac ctacaacgat caccggttgg taatggcttt tgcaccatta gcattaaaga
     2701 caggtgagct ttttctagat gatatttccg gtgtctcaaa atcgttccca gattattttg
     2761 agcagatgaa atctttagga tttaatatag aagaagtttt tttgattatt tcttgtaagc
     2821 aacggagtat ggatttacac ccaactcgca tttatatccc ggaaccaact caagcgtgtt
     2881 catgtctatc tgagttaaat ttccattacg tcctgcgcat gcgccactat gatgtggtgc
     2941 ttcaccattt gcatcaacat ttctgtttgc tacataaaca acacctttgt catcatcaac
     3001 agcaacacca tgtggctgcg ttcctgtatg tatctttttg attaaagtta aattattgta
     3061 atctatcact gcaacagaac cgcgttttcc attaatgata tcctcagtac atgtaacaaa
     3121 taaatagggt ttcttcaatg ataaagaaaa ctcctgtggt aaggtgccta ccggaatatt
     3181 tgcaagcaat gtatgcgtgt tggcatcaaa aacacgcact tcattggtgg catcgcatga
     3241 aataaaatat tttgaataat caggagagaa aattatttca tggccgttga gtccgcctcc
     3301 ataagaataa gtctgaatat tactgagcca ttgcggaaaa tctgatgggc ttccgatagt
     3361 atccacaaca taataagtgc ttccattttg attggtgata taaagtttat ctcctgtgct
     3421 gttaaaagcc ataccatgcg ggtaggctaa atttatgata ggtaaggagg tgaatgatgt
     3481 catcggaaaa ttttcaagat tcaacactgc aatcctgcct gatgcactcc agtcaacact
     3541 ataagcacgt ttactgtccg gtgaaataac caaggtgccc cactgcccgg gcataccatt
     3601 actgaaatca acctgatcta caaagctgtc atcagaagtt ctgtagcgtt gtatgattcc
     3661 tgaactaaaa aaagcaatca gaaaatattt ttcatccggt gtaagtctga tcatgtgtgg
     3721 tgcttcagtg aagcctgcaa tatttcctac atcaaaccat ttcatcaata atcctgatga
     3781 ttcatcaaga actgcaacct gatcgcaagc ctgattggag atgtaaactt tttttctgtt
     3841 gtcgtcttgc ggaaaaatat cctgaccaca tgcatttttt gctccattga ttatccagtt
     3901 ttttatggtt gacacttcgc tgtgagaaag tgatgtttca ttgtatggca tctttggttc
     3961 atttcctgta aatcctaaat cagaatagat attgcagaaa gaaaataatg tactgtaatc
     4021 tgcattgccc ggaatcacta cagcacctgc atcattacct ttaatcatag aagcccaatt
     4081 ggtgagattt agtccgcttg cagcagaatt agaa
//
LOCUS       DAORQT010000133         4109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3923_length_4109_cov_4.525160, whole genome shotgun sequence.
ACCESSION   DAORQT010000133 DAORQT010000000
VERSION     DAORQT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_4109_cov_4.525160"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..880
                     /locus_tag="PK134_05570"
     CDS             47..880
                     /locus_tag="PK134_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB38352.1"
                     /translation="MAKVISIANQKGGVGNTTSAINLAASLAVLEYKTLLIDADPQAN
                     STSGVGFDPRKITNSIYECIINDLDPAKAIQETQTPNLFLLPAHIDLVGAEIEMINLS
                     NREYMMKQVIDKISADYEFIIIDCSPSLGLVTINALTASDSVIIPVQCEYFALEGLGK
                     LLNTINIVQSRLNPNLGIEGILLTMYDSRLRLSNQVVEEVKTHFQNMVFSTIIQRNTK
                     LGEAPSFGETIIMHDASSKGAINYLNLAREILQKNNLTGVPTQREKLFKAEETKKVRM
                     N"
     gene            946..1836
                     /locus_tag="PK134_05575"
     CDS             946..1836
                     /locus_tag="PK134_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRB38353.1"
                     /translation="MSQKKSGLGKGLSALLDNSISVNEFKEIGVATSNAQITTLPVDR
                     IEANPFQPRSEFKEEELLNLADSIRVHGLIQPITVRKLSADKYQIISGERRFRAAQLV
                     GLTNVPVFIRLANDQSMLEMALVENIQRENLNAIEIAISYKRLIDECKLTQEDLGLRV
                     GKDRTTVTNYLRLLKLPPQIQTALRDEKISMGHARAIISISDPVMQLKIFNEILENDL
                     SVRSVEDIVRGSSKNEKKQPSISEKFIKGIEYKHIQKRLEDKFETKIEVKHRKNGSGQ
                     IMFTYFSTDDLNRLLDILDA"
     gene            1849..2445
                     /locus_tag="PK134_05580"
     CDS             1849..2445
                     /locus_tag="PK134_05580"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRB38354.1"
                     /translation="MRLRLLVVLFFTVYCVNAQQDTMIFKTRTNVIVKADSIKPTSDL
                     SQKVNKHSPHKATIYSAILPGLGQAYNKKYWKIPVIYGLTGVLTYFAIDNNKEYVVYK
                     DAYKWRLDDNSATVDKFEGIYSDEDLRILKNYYRRNRDLSFIGMGVVYLLNVVDAAVD
                     AHLFYFDVSDDLSLKVQPGIQPMSHTAFAGVKLSLQIK"
     gene            2449..3162
                     /gene="dapB"
                     /locus_tag="PK134_05585"
     CDS             2449..3162
                     /gene="dapB"
                     /locus_tag="PK134_05585"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRB38355.1"
                     /translation="MKIALLGYGKMGKEIETAAIESGHQIVLKINSANYNQLNKESLQ
                     TADVAIEFSKPEAAIANAKLCVDSNVPVIIGTTGWYEHKATLEQYCIQNNGTVIYASN
                     FSTGVNIFFIINEMLSKLMAPMKYDAWIEEVHHIHKKDIPSGTAITLANDVLKYNKQF
                     SGWAINDEEGKLPIYAQRIDEVTGFHSVSFRNEVDTIEITHNAFSRKGFALGAIKAAE
                     IALTKKGFYEYKNLLIEQP"
     gene            3167..3808
                     /gene="nth"
                     /locus_tag="PK134_05590"
     CDS             3167..3808
                     /gene="nth"
                     /locus_tag="PK134_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRB38356.1"
                     /translation="MLLKERYKYVISYFRKNMPVAETELVYKNPYQLLVAVILSAQCT
                     DKRVNMVTPELFKAFPTAKKLSETEPEEVFEYIKSISYPNNKAKHLVGMARMLLEKFE
                     GIVPSELDDLIQLPGVGRKTANVISSVVFNKAAMAVDTHVFRVAARLGLTRNAKTPLA
                     TEKQLVKHIPEELIATAHHWLILHGRYVCTARNPKCNICGLKDVCVYYKKHPV"
     gene            complement(3805..>4109)
                     /locus_tag="PK134_05595"
     CDS             complement(3805..>4109)
                     /locus_tag="PK134_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRB38357.1"
                     /translation="TKVPGIFRARKKNIGIRIPDQKVTLSIIQQLGHPLMSASLHDVN
                     DPITDYLTDPDEIYERFSDMVDMIVDCGAGGNVPSRIVDCTTDEITVVREGKGVLS"
BASE COUNT         1403 a          744 c          764 g         1198 t
ORIGIN      
        1 gtacaatgac tttttaaaaa tatatacaat agaaaaaata aaataaatgg ctaaagtaat
       61 ttcgattgca aatcaaaaag gaggagtggg aaacaccaca tctgctatta atctggcagc
      121 aagtttggca gtgctcgaat ataaaacact attgattgat gccgacccgc aggcaaacag
      181 tacaagtggt gttggatttg atccacgaaa aatcactaac agtatttacg aatgtatcat
      241 caacgacctt gaccctgcca aagcaattca ggaaacacaa acacctaatc tgtttttact
      301 tcctgctcat attgacttgg ttggtgcaga aattgaaatg attaatctct ctaacagaga
      361 gtatatgatg aagcaggtaa ttgataaaat ttctgctgac tatgagttta ttatcattga
      421 ctgttcacca tcgttaggat tggtaactat caatgcgctc acagcttccg acagcgtaat
      481 tattccggtt cagtgtgaat attttgcctt agaaggtctt ggcaaattgc tcaacacaat
      541 caatattgtt cagagcagat taaatcctaa tctgggtatt gaaggaatct tactcaccat
      601 gtatgactca cgtctgcgac tgagcaatca ggttgttgaa gaagtgaaaa cacattttca
      661 gaatatggta ttcagtacca tcattcagcg taataccaaa ttgggcgaag ctccaagttt
      721 tggagaaaca attattatgc acgatgccag cagcaaaggt gccattaact acctgaatct
      781 ggcaagagaa attctgcaaa aaaataattt aaccggtgta ccaacacaac gtgaaaaatt
      841 attcaaagca gaagaaacta aaaaagttag aatgaactaa attattcctt aaaccgtttc
      901 agattaaaac cttaaaccgg tttcagatta aaaccaaaat atagaatgtc acaaaaaaaa
      961 tcgggtcttg gcaaaggtct aagtgccttg cttgacaaca gcatatcggt aaatgaattt
     1021 aaagagattg gtgttgctac atctaatgcg caaatcacaa ccctgcctgt tgatcgtatt
     1081 gaagccaacc catttcagcc acgcagcgag tttaaagaag aagaattatt aaaccttgcc
     1141 gactctatca gagtacatgg actcattcaa cctattaccg taagaaaact ttctgccgat
     1201 aagtatcaga taatctccgg tgaaagaaga tttcgtgcag cacaattagt tggcttaaca
     1261 aatgttcctg tttttatccg ccttgccaac gatcagagta tgctggaaat ggcattggtg
     1321 gaaaatattc agcgagaaaa cttaaatgct attgaaattg ccataagcta caaaagactc
     1381 attgatgaat gtaaactaac gcaagaagac cttggacttc gtgtaggcaa agaccgtacg
     1441 acagtaacaa actatcttcg tttgttaaag ttgccaccac aaattcaaac tgctcttcgt
     1501 gatgaaaaaa taagtatggg ccatgcacgt gccataatta gcattagcga tcctgttatg
     1561 cagttgaaaa ttttcaacga aattcttgag aatgacttaa gcgttcgctc tgttgaagat
     1621 attgtacgcg gctcatcaaa aaatgagaag aagcagccaa gcatcagcga aaagtttatc
     1681 aaaggaatag agtacaaaca tattcagaaa cgtttggaag ataagtttga aacgaagatt
     1741 gaagttaaac atcgtaaaaa tggttcgggg caaattatgt ttacctattt ttctactgac
     1801 gatttaaatc ggttactcga tattcttgac gcataaattt ttagctgcat gaggttgaga
     1861 ttattggttg ttctgttttt tacggtttac tgcgtaaatg cccaacagga caccatgatt
     1921 tttaagacca gaacaaatgt tattgttaag gccgattcca taaaacccac ttctgattta
     1981 tctcaaaaag tcaataaaca ttctccacat aaggctacaa tctattcagc tattttacca
     2041 ggattaggtc aggcttacaa taaaaaatac tggaagatac ctgtaattta cggactaaca
     2101 ggagtgctta cctattttgc tattgacaac aacaaagagt atgtagttta taaagatgcc
     2161 tataaatggc ggctcgatga caacagtgct actgtagata agtttgaagg tatttactca
     2221 gatgaggatt tgcgaatttt aaaaaactat tatcgcagaa accgcgacct ttcctttatc
     2281 ggaatgggtg ttgtttattt gctcaatgta gttgatgcag cagttgatgc tcacttgttt
     2341 tattttgatg taagcgatga tttatcatta aaagtgcagc ccggcattca accaatgagc
     2401 catactgcat ttgcaggcgt aaaactgagt ttacaaataa agtaagatat gaaaattgca
     2461 ctgttaggat acggaaaaat gggcaaggaa attgaaactg ctgctattga gagtggacat
     2521 caaattgttc taaaaattaa ctctgcaaat tacaatcagc ttaataagga gtctctacaa
     2581 actgccgatg ttgccataga attttcaaag cctgaagctg ctatagccaa tgctaaatta
     2641 tgtgttgatt caaacgttcc tgtaattata ggtacaacag gttggtatga acacaaagca
     2701 acattagagc aatattgtat tcaaaataat ggaacggtta tttatgcttc taacttcagc
     2761 acgggtgtga atatattttt tatcatcaat gagatgttgt ctaaacttat ggcacctatg
     2821 aaatatgatg catggattga agaagtacac cacattcata aaaaagatat tccaagtggc
     2881 acagccataa cactagctaa tgatgtactg aaatataaca aacaattttc tggatgggca
     2941 attaatgatg aagaaggtaa acttcccata tatgcacagc gcattgacga agtaacaggt
     3001 tttcattcag tttcattccg taacgaagtt gacaccattg agattacaca caatgcattc
     3061 agcagaaaag gatttgcact tggtgccata aaagctgcag aaatagcttt gacaaaaaaa
     3121 ggattttacg agtataagaa ccttttaata gaacagcctt gattttatgt tgctaaaaga
     3181 gcgatataaa tatgtaatca gctacttcag gaaaaacatg cctgtggcag aaactgaatt
     3241 ggtttacaaa aatccatatc aacttttagt tgcagttatt ctatctgctc aatgtactga
     3301 caaaagagtc aatatggtta ctcctgaatt attcaaagct tttcctacgg ctaaaaaact
     3361 ttcagaaaca gagcctgaag aagtttttga gtatataaaa tctataagct atccaaataa
     3421 caaggcaaaa catcttgtcg gcatggcgcg aatgctttta gaaaagtttg aaggcattgt
     3481 tccatcagaa cttgatgatc ttattcagtt gcccggtgtt ggaagaaaaa ctgctaatgt
     3541 aatttcatct gttgttttca ataaagccgc aatggctgtt gacacacacg tttttcgtgt
     3601 ggcagcacga ttaggtctga cacgcaatgc aaaaacacca ttagctacag aaaagcaatt
     3661 agtaaaacat ataccggaag aactaattgc aactgcacac cattggctca tacttcatgg
     3721 ccgttatgtt tgtacggctc gaaatccaaa atgtaatatt tgcggattaa aagatgtttg
     3781 tgtttattat aaaaaacatc ctgtttaaga taacactcct ttgccttcgc gcacaacagt
     3841 aatttcatct gttgtacaat ctacaattct actcggcacg ttccctcctg ctccacaatc
     3901 aacaatcata tcaaccatat cgctgaacct ttcataaatt tcatcagggt cggttagata
     3961 atctgtaatt ggatcattta catcatgcag tgaagccgac atcagtggat gccccagttg
     4021 ctgaatgatt gatagagtta ctttctgatc aggaatacga ataccgatat tcttttttct
     4081 tgccctgaat ataccgggca cttttgttg
//
LOCUS       DAORQT010000134         4080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3967_length_4080_cov_5.383354, whole genome shotgun sequence.
ACCESSION   DAORQT010000134 DAORQT010000000
VERSION     DAORQT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_4080_cov_5.383354"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK134_05600"
     CDS             <1..363
                     /locus_tag="PK134_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38358.1"
                     /translation="DNESVMVPPMIIQPIVENAIWHGLKNRKDGGLLTINVDSTKDEL
                     VFEIKDNGCKADARMSEDEKLRNAKRKSMGMQITKERLDIINQSNNANARIIEKEIIG
                     GDGSYQGKVVKLYLPLLD"
     gene            complement(375..1100)
                     /gene="lptB"
                     /locus_tag="PK134_05605"
     CDS             complement(375..1100)
                     /gene="lptB"
                     /locus_tag="PK134_05605"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRB38359.1"
                     /translation="MLLRAENLVKIYKKRTVVNNVSIQVAQGEIVGLLGPNGAGKTTT
                     FYMTVGLIKPNSGKIFLDDNDITALPMYKRAQSGIGYLAQEASVFRKLSVEDNIKAVL
                     EMTKLSQEEQDAKTEELLNEFGLQRIRKSRGDLLSGGERRRTEIARALAVSPKFILLD
                     EPFAGVDPIAVEDIQSIVAKLKEKNIGILITDHNVHETLTITDRAYLLYEGKILKAGT
                     AEDLANDEQVRKVYLGQNFELRN"
     gene            complement(1137..>1488)
                     /locus_tag="PK134_05610"
     CDS             complement(1137..>1488)
                     /locus_tag="PK134_05610"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB38360.1"
                     /translation="FNLMNYYEFVSDQYFAFYATHHFEGFFLDKIPLLKKLKWREVAS
                     LRGVYGSMSQKNHDLMVAPTYNTLSKKPYLEAGFGVENILKIIRVDFIYRLSYLDNPD
                     ISKFGIRGSLQLTF"
     assembly_gap    1489..1498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1499..3606)
                     /locus_tag="PK134_05615"
     CDS             complement(<1499..3606)
                     /locus_tag="PK134_05615"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRB38361.1"
                     /translation="MKTIFQSLAGIALFLLFHSTSLAQLTRISGKVTDALTGEPIPFA
                     SVVFKKSTIGVNTNIDGIYTLESDTPSDSISAQFLGYIPVSLPVRKGQTQTINFLLKA
                     SKVELKEAVIHAGENPANIIIKKVIANRDKNNFEKLNSYSFETYNKLEFDLTNITDKF
                     KNRKIFKPFAFIFDNIDSTTTNEKPFLPIFITESLSDRYLSNNPKDKREIIKASKVSG
                     VENETVTQFLGDMYQNINIYDNFMDVFGKKFVSPISSLGLIYYKYYLLDSLYMGNNHC
                     YKLKFKPRRPEDLTFTGEMFITDTTWAVKKVNMRISNKANINFVEDCAVVKEYEFVDS
                     TMWMLSKDLLVIEFAARDKGLGLIGRKSTSYKNFDLNKPIPPNVMKGSQDVVVLDSAL
                     YKDNSFWDTARHDSLNEREKKIYTMVDTIKSLPAYKTYVDVITLFVTGYKTLGKVDLG
                     PYFTVFSFNEIEGYRFRLGGKTNQDFSKHLRLEGFVAYGTKNEQFNYSFGTRYLFTTK
                     PRMGVGFKYRHDVMQLGQSDNAFQDDNILASLFRRSASNKLTYTESEKIYFEKEWLSG
                     VSGRITFEHNEFIPLGKLDYRYYTNENESNIRDKINTSDISAYFRFAYREKFVESKHG
                     RVSLGSKYPVLQAVYTQGLKGVLRSDFDYQKLIVKVDDYLYTAPIGYLYYVLEAGKVW
                     GKLPYPLLEVHRGNESYFYD"
     gene            3750..>4080
                     /locus_tag="PK134_05620"
     CDS             3750..>4080
                     /locus_tag="PK134_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRB38362.1"
                     /translation="MKDKLSLLEQKKKEALAGGGAKRIDDQHKKGKLTARERIHFLLD
                     EGSFEEIGMLVTHRATDFGLAKEKYLGDGVVTGYGTIDGRLVYVISQDFTLFGGYLSE
                     THAEKICS"
BASE COUNT         1284 a          732 c          747 g         1307 t
ORIGIN      
        1 gataatgaaa gtgtgatggt tccgccaatg attattcagc ctattgtaga gaatgcaatt
       61 tggcatggtt taaaaaacag aaaagatggt ggtcttctta ccattaatgt tgacagcaca
      121 aaagatgaat tagtttttga aataaaggat aatggttgta aagcagatgc ccgaatgtca
      181 gaagatgaaa aactgcgcaa tgccaaacga aaatcaatgg gaatgcaaat cactaaggag
      241 cgtttagata ttatcaatca aagcaacaat gccaatgcca gaataattga aaaagaaatt
      301 attggtggtg atggcagtta tcagggtaag gttgtgaaac tatatcttcc tcttttggat
      361 taaaatcttg ctatttaatt cctaagctca aaattctgtc cgagatatac ttttcgaacc
      421 tgctcatcat tagcaaggtc ttcggcagta ccggctttta aaattttacc ttcgtacagc
      481 agataggcgc ggtcagttat agttagtgtt tcgtgaacgt tgtgatcagt aattaaaatt
      541 ccgatatttt tttctttaag ctttgctaca attgattgta tgtcttcaac agcaatgggg
      601 tcaacacctg caaatggctc atcgagcaga ataaatttag gactcacagc caaagcacgt
      661 gcaatctctg ttcgtctgcg ttcgccaccg gataatagat cacctcggct ttttcttatc
      721 ctctgtaaac cgaattcatt aagaagttct tctgtcttgg catcctgttc ttcttgcgaa
      781 agttttgtca tctccaaaac agccttaata ttatcttcaa cacttaattt gcgaaaaaca
      841 gaagcttcct gagctaaata gccaataccg gactgtgccc ttttatacat tggcaaggct
      901 gtaatatcat tatcatcaag aaaaatttta ccgctattgg gttttatcaa gccaacagtc
      961 atataaaacg ttgtggtttt accggcacca ttggggccaa gcaaaccaac aatttcacct
     1021 tgtgctacct gaatagacac attgttaaca acggttcgtt ttttataaat cttaactaag
     1081 ttttctgcgc gtaacaacat ttttgtcaat tcaatatggt gcaaagataa cagctatcag
     1141 aatgtcaatt gcaaacttcc tctgattccg aatttagaaa tgtcgggatt gtctaaataa
     1201 ctgagacgat aaataaaatc aacacgaatt atctttaaaa tattttctac tccaaaacct
     1261 gcctcaagat agggtttttt agaaagtgta ttgtaggtag gggcaaccat taaatcatga
     1321 tttttttgcg acatgctgcc ataaacacca cgaaggcttg ccacttcgcg ccactttaat
     1381 tttttaagca atggaatttt atctaagaag aatccttcaa aatgatgtgt tgcataaaat
     1441 gcaaaatatt ggtcgctgac aaattcataa taattcatca ggttgaaann nnnnnnnnta
     1501 atcataaaaa taggattcat tgccgcgatg cacttccagt aacggataag gtaatttacc
     1561 ccaaactttt ccggcttcta aaacatagta cagatagccg attggtgcag tatataaata
     1621 atcatccacc ttaacaatca gtttctgata atcaaagtca cttcgtaaaa caccttttaa
     1681 tccttgtgta tatacagcct gcagcaccgg atatttactt ccaagactta cacgtccatg
     1741 cttgctttca acaaattttt cacgataggc aaacctgaag taagcagaaa tatcagacgt
     1801 gttaatttta tctctgatgt tgctttcatt ttcatttgta tagtaacgat aatctaattt
     1861 tcctagtggt ataaactcat tgtgttcaaa agtgattcgt ccactgacac cggaaagcca
     1921 ttctttttca aaataaattt tttcactttc agtataagtt aatttgttgg aggccgatcg
     1981 cctgaaaagc gatgccaaaa tattatcgtc ctgaaatgca ttatcacttt gcccaagttg
     2041 catcacatcg tgcctgtatt taaagccaac acccatgcgt ggttttgttg taaataagta
     2101 acgagtgcca aaactatagt tgaactgttc gttttttgta ccataggcaa caaagccttc
     2161 taaacgcaga tgtttactaa aatcctgatt cgtttttcca ccaagccgaa aacgataacc
     2221 ttcaatttca ttaaaactaa atactgtgaa atacggtccc aaatcaactt tgcccaatgt
     2281 tttatatccg gtaacaaaaa gtgtaattac atcaacataa gttttataag caggtaatga
     2341 tttaatggtg tcaaccatgg tgtaaatttt cttttcacgc tcgtttaacg aatcatgcct
     2401 agccgtatcc caaaaactat tgtctttgta aagtgcacta tcaagtacaa ccacatcctg
     2461 cgagcctttc attacatttg gtggaatagg tttgttcaaa tcaaagtttt tataagatgt
     2521 tgactttcgt cctatgagac caagtccttt atcgcgagca gcaaattcta tcaccaacaa
     2581 atcttttgaa agcatccaca tggtgctgtc cacaaattca tattctttta caacagcaca
     2641 gtcttccaca aagttgatat tggctttatt ggaaatacgc atgtttactt ttttgactgc
     2701 ccaagtagtg tcggtaataa acatctcacc tgtaaaagta aggtcttcgg gtctgcgtgg
     2761 tttaaactta agtttatagc aatgattgtt acccatatac aaactgtcga gcagataata
     2821 tttgtaataa attagtccaa gactcgaaat agggctaaca aattttttgc cgaacacatc
     2881 cataaaatta tcgtagatgt ttatgttttg gtacatatca cccaaaaact gtgttaccgt
     2941 ttcgttctca acaccggaaa ccttacttgc tttaatgatt tcgcgtttgt cttttggatt
     3001 gttagacaga tagcggtcac ttaaagattc tgtaataaaa ataggcagaa aaggtttttc
     3061 gttggtagta gtactatcaa tattgtcaaa gataaatgca aagggtttaa aaattttacg
     3121 gtttttaaat ttgtcggtaa tgttggttaa atcaaattct aatttattgt atgtttcgaa
     3181 ggaataactg ttcagttttt caaaattatt tttatccctg tttgcaataa cttttttaat
     3241 gatgatgttg gcaggatttt ctcctgcatg aatcactgct tctttcagtt caaccttgct
     3301 ggcttttaaa agaaaattga tggtttgtgt ttgacctttt cgaaccggaa gcgatacagg
     3361 aatatatcct aaaaactgag cagagatgct gtcgcttggt gtatctgatt ctaaagtgta
     3421 gatgccatca atattggtgt tgacacctat ggttgatttt ttaaaaacaa cactggcaaa
     3481 gggtataggc tcaccggtga gtgcatcggt aaccttacct gaaattctgg taagttgtgc
     3541 caacgaagtg ctgtgaaaaa gaaggaataa agcaatgcct gcaagcgatt ggaaaatggt
     3601 tttcacaaat tgatttatgt agaatttttt accttgctaa gaacggaaaa aagttcgatt
     3661 ccttgcctga cagggcttta gttttatgtt aaacgggcag ttcttcattt agttttttaa
     3721 gtttgcacaa tagaataatt aacacagcag tgaaagataa attatcatta cttgaacaga
     3781 aaaagaagga agcacttgca ggtggcggtg ccaagcggat tgacgatcag cataagaaag
     3841 gaaaactaac ggcaagagag cggatacatt ttttgcttga tgaaggcagt tttgaagaga
     3901 taggcatgtt agtaacgcat cgtgcaacag actttggcct ggcaaaagag aaatatcttg
     3961 gtgatggtgt tgttacaggt tacggaacaa ttgatggcag gttggtttat gttatttctc
     4021 aggactttac tctatttggt ggatacctta gtgagacaca tgcagaaaaa atttgcagta
//
LOCUS       DAORQT010000135         4059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_3992_length_4059_cov_7.048951, whole genome shotgun sequence.
ACCESSION   DAORQT010000135 DAORQT010000000
VERSION     DAORQT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3992_length_4059_cov_7.048951"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PK134_05625"
     CDS             <1..1473
                     /locus_tag="PK134_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRB38363.1"
                     /translation="MHQIELFMKYPCEVQEELLRKLISTAEHTQWGEHFDYQSIDNFK
                     TFSERVPVVTYDQLQPQVERIMKGEQNILWPTEIKWFAKSSGTTSNRSKFIPVSMESL
                     EECHFKGGKDLLSIYFNNNPDALLFNGKLLSLGGSHSINAFHNESYYGDLSAILIQNL
                     PFWVQIMRTPEMNIALMDKWDEKIERMAQETIKENVTNISGVPSWTLVLLKRILEITG
                     KKTIAEVWPGLELFVHGAVNFTPYREQFKQIIGKHIQYLETYNASEGFFGIQDRNDSD
                     EMLLMLDYGIYYEFIPQKHWHEENPKTLTLSEVQIGEQYALVITTNAGLWRYKIGDTI
                     QFTSISPYRIKISGRTAHFINAFGEELVIDNAEKAITEAGKRTGATVREFTAAPVYFG
                     NKNNGAHEWLIEFSHEPENLDEFTMALDEELKKVNSDYEAKRFKDITLNKPIVHKMPV
                     NTFYKWLEKNKKLGGQHKVPRLANHRKFVDEILNVQNNLS"
     gene            1470..2258
                     /locus_tag="PK134_05630"
     CDS             1470..2258
                     /locus_tag="PK134_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38364.1"
                     /translation="MKYIVTLFIIFIYCNTFAQQQKVNEIWNGADAFSMDNLGNLYII
                     KESVVFKADTVGKILLTYSNNNQDRFTYVDAIDPLRIMLFADEFSIISFLDNKLALQS
                     VINLREQNFYDIKAVCNSTDGFWCYDHSQQQIFKYNFTLQKISETQPLALLINNPLTI
                     TSMCANDKWLACLNKNSGILIFDRLGSYVRSIDSKHASCIMLLENMLVYAENNELVFI
                     DLKLNSETSRLKTDINNISSIKWFHNRFYLLADNKIVQIDYKKD"
     gene            2377..2997
                     /locus_tag="PK134_05635"
     CDS             2377..2997
                     /locus_tag="PK134_05635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRB38365.1"
                     /translation="MHIAIAGNIGSGKTTLTSLLSKQFKWEPHYEDVNDNPYLNDFYD
                     DMQRWSFNLQIYFLNSRFQQILKIKKHPKTIIQDRTIYEDAYIFAPNLHAMGLMTTRD
                     FQNYQQLFELMNSFVSPPDLMIYLRGTVPALVKQIEKRGRPYENNIRIDYLKRLNERY
                     EAWISTYELGKLLIIDIDEINFAENKEHLGMIIERVQAELSGLFSK"
     regulatory      3044..3236
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3372..>4059
                     /locus_tag="PK134_05640"
     CDS             3372..>4059
                     /locus_tag="PK134_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HRB38366.1"
                     /translation="MYKNYLLIIFSIICTNIQAQTDTFHIKVVNIEALRQQDATPGLK
                     FEKIDSISLKQFNTESLSDLLSAESGVLIKNYGPGSLASSSIRGGNANQTAVIWNGFN
                     INNPMLGQTDFSIIPEFFADKISMQYGGGSAQWGSGAVGGAVLLNSSTQFNKGFDAEI
                     NVGAGSYGWLRQSFKTNYSNNNWIFKLKVFNEYSPNNFRFQNSITLQKQLQKLPSTQR
                     QGLLGEDVYRL"
BASE COUNT         1369 a          706 c          769 g         1215 t
ORIGIN      
        1 atgcatcaga ttgagctttt tatgaaatat ccatgcgagg ttcaggagga gttgcttaga
       61 aaacttattt caacagcaga gcatacacag tggggcgaac atttcgacta tcaatctata
      121 gacaatttta aaacctttag tgagcgtgtg cctgttgtta cttatgatca gttgcagcca
      181 caggtggaaa gaatcatgaa aggcgaacaa aatattctgt ggccaacaga aataaaatgg
      241 tttgccaaat catcgggaac aacttcaaac agaagtaagt ttattccggt tagcatggag
      301 tcgctggaag aatgtcattt taaaggcggt aaagacctgc tttcaattta ttttaacaac
      361 aaccctgatg ccttactatt caacggcaaa ctgctttctc ttggtggcag ccacagcatc
      421 aatgcatttc ataacgaatc gtattacggt gatctttctg ctattctgat tcagaacctt
      481 cctttctggg tacagataat gcgaacacca gaaatgaata ttgcactgat ggataaatgg
      541 gatgaaaaaa ttgagcgcat ggcacaggag acaattaagg agaatgtgac taacatttca
      601 ggtgtgccca gttggacatt ggtgttattg aaacgtattc ttgaaattac aggtaagaaa
      661 acaatagcag aagtgtggcc tggtttagaa ctgtttgttc atggtgctgt aaattttact
      721 ccatacagag agcagtttaa acagattatc ggaaaacaca ttcagtattt agaaacctat
      781 aatgcctccg aaggattttt tggtattcag gacagaaacg attctgatga aatgttgctt
      841 atgctcgact atggaattta ctatgagttt attcctcaaa aacactggca tgaggaaaat
      901 ccaaaaacgt tgacactctc cgaagttcaa atcggagaac aatatgcatt ggtcattaca
      961 accaatgccg gtctgtggcg ttataaaatc ggagacacca ttcagtttac atctatcagt
     1021 ccttacagaa taaaaatcag tgggcgaact gcacatttta ttaatgcttt tggagaagaa
     1081 cttgtgattg acaatgcaga aaaagcaatt acagaagcgg gcaaaagaac aggagctaca
     1141 gtgcgtgaat ttactgctgc accagtttat tttggcaata aaaataatgg agcacatgag
     1201 tggcttattg aattttcgca tgagcctgaa aacttagatg agtttacaat ggcacttgac
     1261 gaagagctta aaaaagttaa ttccgattat gaggcaaaac gatttaagga catcacgctc
     1321 aacaaaccta ttgtgcataa aatgcctgtg aatacgttct ataaatggct ggaaaaaaat
     1381 aaaaaattgg gaggacagca taaggtgcca cgtcttgcca accacagaaa gtttgttgat
     1441 gaaatattaa acgtacaaaa caatctgtcg tgaaatatat tgtaacacta ttcatcattt
     1501 tcatttactg caacactttt gcacagcagc aaaaagttaa tgaaatctgg aatggtgccg
     1561 atgctttcag catggataat ctcggcaacc tctatattat aaaagagagt gtagttttta
     1621 aagctgatac cgtaggaaaa attttactta cctacagcaa taacaatcag gacaggttta
     1681 cttatgttga tgcaattgac ccattgcgaa taatgctttt tgctgatgaa ttttctatca
     1741 tcagtttttt ggataataaa ttagccttgc aatcggtaat taatttaaga gaacaaaatt
     1801 tctatgacat aaaagctgtt tgcaattcta cagatgggtt ttggtgctat gatcactcac
     1861 aacaacaaat cttcaaatat aattttacac ttcagaaaat ttcagaaaca caaccgttgg
     1921 cattattaat aaacaatccg ctaacgataa cgagcatgtg tgccaatgat aaatggctgg
     1981 catgtttgaa taagaattca ggaattttaa tttttgacag gctgggaagc tatgtaagaa
     2041 gtattgattc aaaacatgca tcatgtatta tgctactcga aaacatgttg gtttatgcag
     2101 aaaacaatga actggttttt attgacctga agctgaattc agaaacatca agactgaaaa
     2161 ctgacatcaa taacattagc agtataaaat ggtttcacaa ccgtttttac ctgcttgccg
     2221 acaataaaat agtacagata gactacaaaa aagattaaaa ctgtagaatt ataaaggcta
     2281 ttacttaagt aactgctttt gccaatgaaa ttttttttac tttagcgcac tactttttga
     2341 gaactatatt atttaaaacc agattgattt aattgtatgc atattgcaat agcgggaaat
     2401 attggatccg gaaaaactac actcaccagt ttattgtcaa aacagtttaa atgggaacca
     2461 cattacgaag atgtaaatga caatccctat ctgaacgact tttatgatga catgcaacgc
     2521 tggtcgttca accttcaaat atattttctg aacagccgct ttcagcaaat tttaaaaatc
     2581 aagaaacacc caaaaacaat tattcaggac agaacaatat acgaagacgc atatattttt
     2641 gctcctaacc ttcatgcaat ggggttaatg acaacacgcg attttcagaa ctaccaacag
     2701 ttgtttgagt tgatgaacag ttttgtttca ccacctgatt tgatgatata tctgcgtggc
     2761 accgttccgg ctttggtaaa acaaattgaa aaacgcggac gaccttatga gaacaatatt
     2821 cgcatagatt atctgaaacg tttgaacgaa cgttatgaag catggatatc aacttatgaa
     2881 cttggcaaac tgctgattat tgatattgat gaaatcaact ttgctgaaaa caaagagcat
     2941 ctcggaatga ttatcgagcg tgtgcaagca gaactcagcg gtttgttcag caaataagct
     3001 ccctttattt ttttatttat atttgccacc ggaagttctt tcattcattt caaccggtaa
     3061 aggtttcttc tgttttagac tgaagatata aaagggaatc acgttaaaat cgtgagctgt
     3121 agcgcaactg taagtaacgc ataatttatc tctgctgaat ccactgcaaa atttgcagga
     3181 aggaatagag ttaaaagtta caagccagga tatctgtctt tactgaggct gtttattcag
     3241 ccgttgacca tgctttcgca ttaaaagcag aagtctgtat tgatacaact ttgaacctag
     3301 gttgtattac tcctcacttt tttatttact gcactgcatg tagaaatgct tgacgaaaaa
     3361 ccaagcaaaa catgtataaa aattatctgc tgattatttt ctctattatc tgcaccaaca
     3421 ttcaggcgca gacagatact tttcacatca aggtggttaa tattgaagca ttgcgacaac
     3481 aggatgctac tccgggatta aaatttgaaa aaattgacag catcagcctg aagcaattca
     3541 atacggaatc gttaagcgat cttctttctg ctgaaagtgg tgttcttatt aagaattacg
     3601 gacctggttc gttagcttct tcttccatca gaggtggaaa tgccaatcag actgcagtaa
     3661 tctggaatgg atttaacatc aacaatccta tgctaggtca aaccgatttt tccattatcc
     3721 ctgaattttt tgctgataaa ataagtatgc aatatggtgg cggcagtgca caatggggaa
     3781 gtggcgctgt aggtggtgct gttctgctca attcatcaac acaattcaat aaaggctttg
     3841 acgcagaaat aaatgttggt gccggtagtt atggatggtt gcgacaatct tttaaaacta
     3901 actacagcaa caataattgg atttttaaac ttaaagtatt taatgaatat tctccaaata
     3961 attttcgttt tcaaaattct attacgcttc aaaagcaatt acaaaaactt ccaagtactc
     4021 aacggcaagg tttgttaggt gaagatgtat acagattga
//
LOCUS       DAORQT010000136         4046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4018_length_4046_cov_5.332999, whole genome shotgun sequence.
ACCESSION   DAORQT010000136 DAORQT010000000
VERSION     DAORQT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_4046_cov_5.332999"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK134_05645"
     CDS             complement(<1..972)
                     /locus_tag="PK134_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRB38367.1"
                     /translation="MKKKVVSVIKYALFLAAGVGMLWLTFRNQDFTQVIQKIENVHIG
                     WLLASVAAAILAHISRAIRWNMLITPLGYKPKLINTFWALCAGYFANLAVPRLGEITR
                     CGMLTKAEQVPFNELFGTVIVERIMDVLMLFLLLLAVATLEFHLIGGFLYDNIAAPLM
                     SKFQNLNLPLVIGVLVVGIIAVFAYFYFSKHSGKSKAIFEKINLLLKGVADGIKSIAK
                     IKNKSLFIFHTLFIWVMYFFASYLCFFALDSTSMLDAKAGLFILVIGGLGMSAPVQGG
                     IGAFHFIVSRGLMLYGISETEGIVYATIVHGYQTILVIALGAIGFFVL"
     gene            complement(986..1336)
                     /locus_tag="PK134_05650"
     CDS             complement(986..1336)
                     /locus_tag="PK134_05650"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRB38368.1"
                     /translation="MFITVLKSKIHRAKVTQAELHYVGSITLDENLMDAADLIENEQV
                     QVLNINNGERFVTYVIKGERNSGVVCLNGPAARRVQLGDIVIVASYAMMEKEEAKNFK
                     PVLVYPDEKNQPVK"
     gene            complement(1358..2797)
                     /locus_tag="PK134_05655"
     CDS             complement(1358..2797)
                     /locus_tag="PK134_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRB38369.1"
                     /translation="MKLLNYINGQMVEPISQKWMDNTDPSTNTVFSQIPDSDERDVQM
                     AVNAAKQAFVGWSTMPVKKRSDLLLRIAALIEKNLQRFAEAESLDNGKPVWLAKQVDI
                     PRASANFHFYATAALHLAAETHAMENTAINYTLRQPIGIAGCISPWNLPLYLFTWKIA
                     PALASGNCVVAKPSEITPVTAFLLSQFCIEAGLPAGVLNIIHGTGPRVGSAIVSHPDI
                     PVISFTGGTKTGAEISRIAAPMFKKLSLELGGKNPNIIFEDCDFDHAVSTSIKSSFSN
                     QGEICLCGSRIFIQRSIYDKFKEAFISKVKGLKTGDPKDESNFLGAIVSQPHFEKILS
                     YIDIAKQEGGVILTGGKAIQPEGRCKDGFFIEPTVIENLPYNCRTNQEEIFGPVVTLT
                     PFDTEEEVLMMANSTNYGLAATVWTTNLTKAHKVAAALHSGIVWINCWLLRDLRTPFG
                     GVKQSGIGREGGFEALKLFTEEKNICIKL"
     gene            complement(2804..>4046)
                     /locus_tag="PK134_05660"
     CDS             complement(2804..>4046)
                     /locus_tag="PK134_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38370.1"
                     /translation="PHTYFEKQQFVPLLQEPPHSQLQCIVVIPCFNEPDLITTLDSLW
                     KCDETKFPVEIIAVINSGRNADSNVMDCNNLTLKTMQLWMQKHNSNHRHYQVIHCSDL
                     PPKKAGVGLARKIGMDEAAYRFSLNGNDNGIIVCLDADCTVQPNYLVEIEKHFSNNIK
                     TAGCSVYFEHPLEGKQEPSIYDAIINYELYLRYYVGALRYCGFHAPYHTIGSSMAVTN
                     KAYQKQGGMNQRKAGEDFYFLHKIFHLGNFSELNTTTVYPSPRISNRVPFGTGSAISK
                     IVTAGNNNYPVYAFETFEILKVFFDQIKVNATSREMINIDSFAPALKNFLSAHNAASK
                     MIEIATQTINSKTYLQRFAHWFDGFMVLKCVHYMRDHFLEQEEVLVATKKLMNNLNIS
                     PGNLTKREMLLRLRKHDRSGS"
BASE COUNT         1261 a          824 c          728 g         1233 t
ORIGIN      
        1 caatacaaag aatcctatag cacccaaagc tatcactaaa atggtttgat agccatgaac
       61 aatggttgca taaacaattc cttctgtttc agaaatacca taaagcatca gtccgcgtga
      121 aacgatgaag tgaaaagccc caattccgcc ctgtacaggt gcagacattc ccaatccacc
      181 tatgacaaga ataaacaaac ctgcttttgc atcgagcata gatgtggaat ccaatgcaaa
      241 gaaacacaga taggaagcaa aaaaatacat cacccaaata aatagggtgt ggaaaataaa
      301 caatgatttg tttttaattt ttgctattga cttaatgcca tcagcaacac ctttaagcaa
      361 taaatttatt ttttcgaaaa tggctttgct ctttcctgaa tgttttgaga aatagaaata
      421 agcaaaaact gcaatgatgc caacaactaa aacacctatt actaatggaa gatttaggtt
      481 ttgaaattta ctcatcaaag gtgcagcaat attatcatat aaaaaaccac caatgagatg
      541 aaactctaat gttgcaacag caagcaataa caaaaacaac atcagtacat ccatgatacg
      601 ttctacaatc accgttccga acagttcgtt aaaaggtact tgctcggctt tggtgagcat
      661 gccacagcga gtgatttcgc ccagacgtgg tacagccaga ttggcaaaat atcctgcaca
      721 taatgcccag aaagtattaa tcagttttgg cttataacct aaaggtgtaa tgagcatatt
      781 ccatctgatg gcacgactaa tatgtgcaag tattgctgca gcaacagatg ccagcagcca
      841 accgatatgt acattttcaa ttttctgaat tacctgtgta aaatcctgat tacggaaagt
      901 aagccatagc ataccaacac cggcagccag aaacaatgca tactttatta ctgaaacaac
      961 ttttttcttc aacctatgtc gggatttatt ttacaggttg atttttttca tcaggataaa
     1021 ccaagacagg tttaaaattt ttggcttctt ctttttccat catggcataa gaagctacaa
     1081 taacgatgtc gcctaactgc accctgcgtg ctgcaggacc attgaggcaa acaacaccac
     1141 tgttgcgttc tcctttgata acataggtaa caaatctttc accattatta atattaagca
     1201 cctgcacctg ttcgttttca atcaggtcag cggcatccat caggttttca tcaagggtaa
     1261 tacttcccac ataatgtaat tcggcctgtg tgaccttagc ccgatgaatt ttcgatttta
     1321 atacagttat aaacatagtg gcggcaaaag taataggtta aagtttaatg cagatgtttt
     1381 tttcttcggt aaataacttt aatgcttcaa aaccaccttc acggccaata ccgctttgtt
     1441 ttacacctcc aaaaggtgtc cgcaaatcac gtaaaagcca gcagtttatc catacaatac
     1501 cactatgaag ggctgctgcc actttatgtg cctttgtcag attggttgtc caaactgttg
     1561 cagccaatcc ataatttgtg ctattagcca tcattaaaac ttcttcttct gtgtcgaaag
     1621 gagttagtgt cacaacgggt ccaaaaatct cttcctgatt ggtgcgacag ttataaggca
     1681 gattttcgat tacggttggc tcaataaaga aaccatcttt acagcgtcct tcaggttgta
     1741 tggccttgcc tccggttaaa attacacccc cttcctgctt ggcaatatca atatatgaaa
     1801 gtattttttc aaaatgtggt tgagatacta tagctcccag aaaatttgac tcatcttttg
     1861 ggtcgcctgt tttcagtcct ttaactttgc tgataaatgc ttctttgaat ttatcataaa
     1921 tgctgcgttg aataaatatg cgtgagccgc aaaggcaaat ttcgccctga ttgctgaaag
     1981 aagattttat tgaagtagaa acagcatgat caaaatcgca atcttcaaaa atgatattgg
     2041 gatttttgcc tcctaattca agagaaagtt ttttaaacat tggcgcggca attcttgaaa
     2101 tttctgcacc agtcttggtg ccgcctgtaa aactaattac aggaatatca ggatgagaaa
     2161 ctatagcgct gcccacacgt ggacctgtac cgtgaataat atttaaaaca ccggctggaa
     2221 gtccggcttc aatgcaaaac tgacttagta aaaatgctgt taccggagta atttcgctgg
     2281 gctttgccac cacacagttt ccagatgcta atgcaggtgc aattttccag gtaaataaat
     2341 acaaaggcaa gttccagggt gaaatacaac ctgcaatacc tattggctga cgtaaagtat
     2401 aattaattgc cgtgttttcc atggcatgtg tttcggcagc aagatgcaat gcagcagtag
     2461 cataaaaatg aaaatttgca gatgcacgtg gaatgtccac ttgtttggca agccaaacag
     2521 gtttgccatt atcgaggctt tcggcttcgg caaatcgttg caggtttttt tcaatcagtg
     2581 ctgctattct cagcaataaa tcagagcgtt tttttacagg catagtactc catcccacaa
     2641 aggcctgctt tgctgcattt actgccatct gaacatcacg ttcatcgctg tcgggtattt
     2701 gtgaaaaaac tgtgttggta gatgggtctg tattatccat ccatttctga ctgatgggtt
     2761 ctaccatctg cccgttaatg taattcaaca atttcatgac ttctcaacta cctgaacggt
     2821 cgtgcttacg caaccttaac aacatttcac gtttagtcaa attaccggga cttatattca
     2881 aattgttcat caattttttt gtggcaacca aaacttcctc ttgctccaga aaatgatcac
     2941 gcatataatg aacgcatttt aaaaccataa aaccgtcaaa ccaatgagca aaccgttgca
     3001 gataggtttt gctgttgatt gtctgtgttg ctatttctat catctttgat gctgcattgt
     3061 gtgccgataa gaaattcttc aaagcaggag caaatgaatc aatatttatc atttctcttg
     3121 atgttgcatt tacttttatc tgatcaaaaa atactttaag aatttcaaaa gtttcaaatg
     3181 cataaacagg ataattatta ttaccggctg ttactatttt tgaaatggca ctgcctgtac
     3241 caaatggaac tctgttactg atgcgcggtg aaggataaac ggttgttgta ttaagctcag
     3301 aaaaatttcc gagatgaaat attttatgca gaaaataaaa atcttctccg gcttttcgct
     3361 gattcattcc tccctgtttc tgataggctt tatttgttac agccatactt gagccaatgg
     3421 tgtgatatgg agcgtgaaaa ccacaatagc gcaatgcacc aacataatat ctcagataca
     3481 attcgtaatt gatgatagca tcataaattg aaggctcctg ttttccttcc agaggatgtt
     3541 caaaataaac agaacagcct gcagttttta tattgttgct gaaatgtttt tcaatctcaa
     3601 ccaaataatt tggctgcact gtacaatcgg catccaaaca aacaattatt ccattatcat
     3661 taccatttaa actaaaacgg tatgctgctt cgtccatacc aattttacgt gcaagcccga
     3721 cacccgcttt tttaggaggc aagtcgctac aatgaataac ttgatagtgc ctgtgatttg
     3781 aattgtgttt ttgcatccaa agttgcattg tctttagtgt aaggttatta caatccataa
     3841 cattggaatc agcatttctt cctgagttga taacagcaat aatttcaaca ggaaattttg
     3901 tttcatcaca tttccacagc gaatcaagcg ttgtaattaa atcaggttca ttaaaacaag
     3961 gaataactac aatgcattgg agctgtgaat gtggaggctc ctgcagtaaa ggaacaaact
     4021 gttgcttttc gaaataggta tgtggc
//
LOCUS       DAORQT010000137         4043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4020_length_4043_cov_5.243480, whole genome shotgun sequence.
ACCESSION   DAORQT010000137 DAORQT010000000
VERSION     DAORQT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4020_length_4043_cov_5.243480"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..>1100)
                     /gene="bshA"
                     /locus_tag="PK134_05665"
     CDS             complement(19..>1100)
                     /gene="bshA"
                     /locus_tag="PK134_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRB38371.1"
                     /translation="LGKALAAKGHRIHFITYSQPVRLDSFSENISYHEVSVSNYPLFE
                     YAPYESALTSMLVDVAMHEGLDVLHVHYAIPHASVAYLAKQILISKGIYIPVITTLHG
                     TDITLVGRDNSYEPVVTFAINQSDGVTAVSESLKRDTYANFEVTKHIEVIHNFIDLKR
                     FSKKPKDHFKRAIAPGGERLLVHTSNFRKVKRVNDVIQVFAKIHKRIPSRLLMIGDGD
                     ERAGAEKLCRDLQLCDEVRFLGKQDAIEEILSVCDLFIMPSETESFGLAALEAMACQV
                     PVISSNTGGMPELNLDGVTGFTSNVGDIEDMAEKAYHILKDDQTLNIFKQNALKRASD
                     FDINVILPRYENYYQSVIQNFTVAP"
     assembly_gap    1101..1113
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1227..>2141)
                     /locus_tag="PK134_05670"
     CDS             complement(1227..>2141)
                     /locus_tag="PK134_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRB38372.1"
                     /translation="EYYLPDYEKTNKGLLTVEQLMTHTAGLQAWIPFYKEGLTKNGKM
                     MKKYFSSSKHDDYTVQISDSLFMNPKFYSRIDKQIKESPLTEKGKYVYSDLGMLLLQK
                     IIERQAGKKMDVLLNEEFYQPLGLSHLLFNPRSKFPLADIVPTEDDTVFRQTLVHGYV
                     HDPAAALLGGVAGNAGLFANAQSVGVLMQMLLNEGSYAGKQYISKATVDTFTKAPIPG
                     IRRGLVFDKPETNPAKGSPTCKSASPQTFGHQGFTGTCTWADPSNKLVYVFLSNRVFP
                     SAANQKISKLSVRTEIQQVFYDCLRKVR"
     assembly_gap    2142..2151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2152..>4043)
                     /locus_tag="PK134_05675"
     CDS             complement(<2152..>4043)
                     /locus_tag="PK134_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRB38373.1"
                     /translation="DEMTVDEKIGQLFMVQMQSVWPEKDLDSLESLIRNYHLGGLIVF
                     KGGPYRQALCINRMQQAAKIPLSVSIDGEWGLSMRMDSTIRFPRQMTLSAMHDDSLLN
                     DAFSEIASQCKRMGIQLNFAPDIDINNNPQNPVINTRSFSDDKFEVAEGGILYMQALQ
                     NHHVLACGKHFPGHGDTDTDSHYALPVINKSANELDTLEWFPFRQLIRNGVSSIMTGH
                     LNVPLLDDENYPASLSKRIINGYLKDDLDFKGLVITDALNMKGAIVADAKPGDIELQA
                     LLAGNDILLMSENVKPAIEKIKTAIQKRKLRKSALDEKVRKILMAKHWAGLYNYTPVD
                     TTNLYYDLNNADASYLNYRLYEKSLTLLRNDAVLPLQNSATERMASLVINDTLENTFQ
                     QTLSLFAPVQNFVMSKDASFATVDSIAAVLSGFKKVIISIHNTTTKPQLNFGITESMI
                     SVIQKISMRTKTIVVLFGNSYCLNKLVIPEQTKALIIAYEDTYLPQHITAQALFGAVP
                     FNGRLPVTPGLSYHRGDGLNLQATGVVKYTMPAELNIDLTCLQKVDSIVNDAIKQKAF
                     PGCQVYASVDGKVIYNKSFGYTSYADTQAVENDMIYDIASVTKINATALAAMWLYDHH
                     LLDLKQT"
BASE COUNT         1239 a          834 c          707 g         1240 t
ORIGIN      
        1 atataaaaat gccggtattt atggcgcaac cgtaaaattc tgaattacgg actgatagta
       61 attttcgtac cgcggaagga taacgttaat atcaaaatca gaagctcttt tcaaagcatt
      121 ttgcttaaaa atattaagtg tctgatcgtc ctttaatatg tgatatgctt tctccgccat
      181 atcttcaata tcgccaacat tacttgtaaa tcctgtcacg ccatctaagt tcagttccgg
      241 catgccaccg gtgtttgatg aaataaccgg cacctgacaa gccatggcct ccaatgcagc
      301 aagtccaaaa ctttcagttt cactgggcat gataaataaa tcgcaaaccg ataatatttc
      361 ttcaatagca tcctgcttac ctaaaaacct gacttcatca cacagttgca aatcgcgaca
      421 aagtttttct gcacctgcac gctcatcgcc atcaccaatc atcaataacc gtgaaggaat
      481 tcttttatgg atttttgcaa aaacctgtat tacatcattc acccttttaa ctttccggaa
      541 attagaagta tgaaccaaaa gtctttcacc accgggagca attgcacgct tgaagtggtc
      601 tttaggtttt tttgaaaaac gtttcaggtc aataaaatta tggatgactt caatatgttt
      661 ggtcacttca aaatttgcat aggtatcacg ctttaaactt tcagaaactg ctgtaacacc
      721 atcactttgg ttaattgcaa aagttacaac aggctcataa ctgttatcgc ggccaacaag
      781 tgtaatatct gtaccgtgta aggtcgttat aaccggaata taaattccct ttgaaattaa
      841 aatctgctta gccagatatg ctactgatgc atgtggaata gcataatgca catgcaatac
      901 atctaaccct tcgtgcatag ccacatcaac caacatactt gttaatgccg actcataagg
      961 tgcatattca aacaacggat aattgcttac cgatacttcg tgataagaaa tattttcaga
     1021 aaaagaatcg agccgcacag gctgactgta ggtgataaaa tgaatgcgat gacctttagc
     1081 agcaagtgct ttgccaagtt nnnnnnnnnn nnnctacacc actaccccca aaggtgggat
     1141 aacaaactat tcctattttc atactgtaag aatcaatcaa caagcaaatt gttgatttgt
     1201 ttagcaggtt gtaaaaatag tttctttcag cgtacttttc gcaaacaatc gtaaaacacc
     1261 tgctgtattt ctgtccttac acttagctta ctgattttct gattagctgc agaaggaaac
     1321 acacggttag atagaaaaac atataccaat ttattggaag ggtcggccca ggtgcaagtg
     1381 cctgtaaatc cctgatgacc gaaagtttgt ggtgatgcac ttttgcatgt agggcttcct
     1441 tttgcaggat ttgtttcagg tttatcaaat accaaacctc ttcttattcc gggaataggt
     1501 gcttttgtaa aagtatcaac agtagcctta ctgatatatt gcttaccggc atagctacct
     1561 tcgttgagaa gcatctgcat gagcacacca acgctctgcg cattggcgaa caagccggca
     1621 ttgccggcaa cgcctcctaa caaggctgct gccggatcgt gcacataacc atgcaccaat
     1681 gtttgcctga acacagtgtc gtcttccgta ggcacaatgt ctgccaaagg aaatttactg
     1741 cgaggattaa acaataaatg acttaacccc aatggctgat aaaactcttc gttgagcaat
     1801 acatccattt tttttcctgc ctgcctctca ataatctttt gcaataacaa cattcctaaa
     1861 tcagaataca catatttacc cttttctgtt aaaggcgatt ctttaatctg tttatctatt
     1921 ctggaataaa acttaggatt cataaaaaga ctgtccgata tctgaacagt atagtcatca
     1981 tgctttgatg aagaaaaata ttttttcatc atcttgccat tcttggtaag cccttcttta
     2041 taaaatggaa tccatgcttg tagtcctgca gtatgcgtca taagctgctc tactgttaac
     2101 aatcctttat ttgtcttttc gtaatccggc aaataatatt cnnnnnnnnn ngtttgtttt
     2161 aaatctaaaa gatgatggtc ataaagccac atggcagcaa gtgcagtggc attaattttt
     2221 gtcacagagg caatatcata aatcatatca ttctccacgg cctgtgtatc tgcataagaa
     2281 gtatagccaa acgatttgtt ataaataacc ttaccatcaa ccgatgcata tacctgacat
     2341 ccaggaaatg ctttctgttt tattgcatca tttactatag agtcaacttt ttgtaggcac
     2401 gtaagatcaa tatttaactc cgctggcatt gtatatttca caacacctgt tgcctgaaga
     2461 ttaagaccgt caccacgatg ataggacaag cccggtgtta caggcaacct gccattaaaa
     2521 ggaacagcac caaacaatgc ttgtgctgtt atgtgttgcg gcagataggt gtcttcatag
     2581 gcaattatta aagctttagt ctgctctgga ataactaatt tattcagaca ataggaatta
     2641 ccaaacaaca caacaatggt tttggtgcgc atagaaattt tctggataac tgaaatcata
     2701 ctttccgtga taccaaaatt taactgaggc ttggttgttg tgttatggat actaatgata
     2761 acttttttaa atccagacag aactgcggca atgctgtcca cggtggcaaa agatgcatct
     2821 tttgacatta caaaattctg aacaggcgca aaaagcgaca atgtttgctg aaatgtattt
     2881 tcaagagtgt cgttaattac taatgatgcc atcctttcag tagcactatt ttgaagtggt
     2941 aaaacagcat cattgcgaag taatgtcagc gacttttcat acaacctgta gttgagataa
     3001 gatgcatcgg cattatttaa atcataataa agatttgtag tgtcaacagg agtataatta
     3061 tataagcctg cccaatgttt ggccatcaga atttttctga ctttttcatc aagagcactt
     3121 ttacgtaatt ttcttttctg aattgctgtt ttaatttttt caatagcggg tttcacattc
     3181 tccgacatta ataaaatatc atttcctgcc agcaaggcct gcaattcaat atcacccggt
     3241 ttggcatcag ctacaatggc acctttcata ttcaaagcat cagtaataac gagtccttta
     3301 aagtcaaggt cgtccttaag atagccattg ataattcttt tagataatga tgcaggataa
     3361 ttctcatcgt caagtaatgg aacattcagg tgacctgtca taatactgct tacaccattt
     3421 ctaataagct gtctgaaagg aaaccactct aaagtgtcta attcattggc cgatttatta
     3481 attacaggca aagcataatg tgaatctgta tctgtatcac catggccagg aaaatgttta
     3541 ccacatgcaa gcacatgatg attttgcaaa gcttgcatat aaagaattcc gccctctgct
     3601 acttcaaatt tatcatcact aaatgagcgt gtattaatta cagggttctg agggttgtta
     3661 ttgatatcta tgtcaggagc aaagttgagt tgaataccca tacgtttaca ttgagatgca
     3721 atttcagaaa atgcatcatt gagcaaactg tcatcgtgca tggcggacaa agtcatctga
     3781 cgtggaaagc gaatggtgct gtccatgcgc atactaagtc cccattcgcc atcaatactc
     3841 accgacaaag gaattttagc tgcctgctgc attctgttaa tacaaagtgc ctggcggtat
     3901 ggtcctcctt taaaaacaat taatcctccg agatgataat tacgtatcaa tgactctaat
     3961 gaatctaaat ctttttcagg ccaaacactt tgcatctgta ccataaaaag ctgaccaatt
     4021 ttttcgtcaa cagtcatttc gtc
//
LOCUS       DAORQT010000138         4035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4033_length_4035_cov_5.283417, whole genome shotgun sequence.
ACCESSION   DAORQT010000138 DAORQT010000000
VERSION     DAORQT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4033_length_4035_cov_5.283417"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1517
                     /locus_tag="PK134_05680"
     CDS             81..1517
                     /locus_tag="PK134_05680"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRB38374.1"
                     /translation="MTENENFQKIKHLYNRAGFGLHYSELQKLSRTKLNKLVADMIEK
                     AKTDVPITTIAPGHVKSVMLKNAGLTKKEGLSPEQLKERQKLLVSLNKQLKNLNTEWF
                     FRMMDIDSPLREKMTLFWNNHFACREEGNPYFAQVLNNIQRKHALGDFRQLLLEVSQS
                     ASMLNFLNNQQNKKGRPNENFARELMELFTLGRGNYSEKDIKESARAFTGWVHDAEGN
                     FVFNTKNHDNGIKTFFGKEGNFNGEDIIDMILQKPEAAVFIARKAYRFFVNDVPDETH
                     VQELGNHFYKSKYDISVLMNKMFTADWFYAPENTGNKIKSPVEFIVGLSRQFNVTYKR
                     QEIILQLQRGLGQTLFYPPNVAGWAGGRNWIDSSSLMLRLTIPSQILNDGSLNFSAKT
                     DPDDENIVAMKQMDDAVIEAKKKGSRIEINWENFWADFPKDYSVQQIAGFILQCKPSA
                     QLNQIISSSIDKKNAIIKTASSPEYQLC"
     assembly_gap    1646..1677
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1678..2756
                     /locus_tag="PK134_05685"
     CDS             <1678..2756
                     /locus_tag="PK134_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRB38375.1"
                     /translation="VIIQLSGGNDGLNTIVPYGNDVYYQLRNTIAIPQSEIIKLNDMQ
                     GLNPTLAPLKEIYDQGWMSIINNVGYPNPNRSHFRSMDIWQTASDSNQFLSTGWIGRY
                     LDSNCQMCKYPYNAIEVDDTLSLTLKGDSKKGIALQDPDKLFRTTNTPFFGKLIEHDK
                     EHLDEDNLGYLYKTMIETYSSASYIKETTKNYDNNFVYPESSFAKKLKTVARFILSGL
                     DTRVYYVSLSGFDTHVNQMAQQKKLFTQFSEGMNAFVKELNNNKKLEDTLVIAFSEFG
                     RRVAQNASRGTDHGTANVMMLFGGQLKKQGIYNDAPDLNDLDAGDLKYKVDFREIYAT
                     VLNKWLNVDNKLILPDSTGTMDFI"
     gene            complement(2774..>4035)
                     /locus_tag="PK134_05690"
     CDS             complement(2774..>4035)
                     /locus_tag="PK134_05690"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRB38376.1"
                     /translation="INIVDLDNDNQKDILVSNLYDSVYWYKNNGLLFNRMSAISNTVK
                     RPYFVDCADVDGNGLQDVLVSYDDVYAGLKLFKNFGGNTWQQIIINDSIDAFVVRSYL
                     VDLDNDSDLDIISCQDLEVAIYEQSAGTFSGMIKVADSSEFYNLVVHDFNGDGFVDFS
                     VHSAAGFRLFINNHNLTFSPQTFTTALYLTLESCDIDNDGDFDLFYPDSAVSFNLITL
                     KNDGSGGFSNFQSVMYHAGNNQNPPLKFLTLDNDTFPDALYIPLVNRGLYYKSNDGVG
                     GLNFSHFIDSVYRYTFVNGGDLDGDNDNDLVWFANEPFGIKRVGYTLNNSGNTDYNEL
                     SSQEFYFYPNPVSNNILNIKYFSPTSSGGVFEIVDAIGKTVFKYTLPLYSSNHQITLP
                     QLQNGVYNAMLTYEGAKICRKLAIVDN"
BASE COUNT         1411 a          672 c          724 g         1196 t
ORIGIN      
        1 ctattatcat tcttaccttt atttttgttc agactaaacc ttctttacaa tatcgtgtca
       61 aatacacagt ttgttttgtt atgactgaaa acgaaaactt tcaaaaaata aagcaccttt
      121 ataaccgtgc cggttttgga ttacactatt ctgagctgca gaaactaagc cgcacaaagc
      181 tgaataagtt agttgccgat atgattgaaa aagcaaagac cgatgtacca attacaacca
      241 ttgctcccgg ccatgtaaaa tctgtgatgc ttaaaaatgc aggacttacc aagaaagaag
      301 gcttgtcacc ggagcaacta aaagaaaggc aaaaactttt agtgagcctt aacaaacaac
      361 ttaaaaacct gaatacagaa tggttcttcc gcatgatgga tattgacagc ccactacgcg
      421 aaaaaatgac cttattctgg aacaatcact ttgcatgtcg cgaagaaggg aatccatatt
      481 ttgcacaagt gctcaataac attcagcgca aacatgcatt aggtgatttc aggcagttgt
      541 tattggaagt atctcagtcg gcatctatgt taaatttttt aaataatcag caaaataaaa
      601 aaggcagacc taatgaaaac tttgcacgcg aactgatgga gttgtttaca cttggtcgtg
      661 gtaactattc agaaaaagat attaaagaat cagcacgtgc atttactggt tgggtgcacg
      721 atgcagaagg aaattttgtg ttcaacacca agaatcacga caatggcata aaaacatttt
      781 tcggaaaaga aggcaacttc aacggtgaag atattattga tatgattttg caaaagcctg
      841 aggctgctgt atttattgca agaaaggctt atcgcttttt tgttaacgat gtgcctgatg
      901 aaacacatgt gcaggaattg ggtaaccatt tttataaaag caagtacgat atttctgtac
      961 tgatgaataa aatgtttact gccgattggt tttatgctcc tgaaaacaca ggtaataaaa
     1021 ttaaatctcc ggtagagttt atagttgggc ttagtcgtca gtttaatgtt acttacaaaa
     1081 ggcaggaaat tattttacaa ctgcaaagag gtttggggca aacattattt tatcctccta
     1141 atgttgcagg ttgggcaggc ggtcgaaact ggattgacag ctcatcattg atgttgcgat
     1201 taacaattcc ttctcagatt cttaatgatg gaagtttaaa tttttctgct aaaaccgatc
     1261 ctgacgatga aaacattgtg gcaatgaaac aaatggatga tgctgttata gaagcaaaaa
     1321 agaaaggttc aagaatagaa attaactggg aaaatttttg ggcagatttt ccaaaagatt
     1381 attcagtgca gcaaatagca ggttttattc tgcagtgtaa gccgagtgca cagttgaatc
     1441 aaatcatcag tagttctata gataagaaga atgcaattat aaaaacggca agctctccgg
     1501 agtatcaatt atgctgaagc aaaaaaataa aaagcaacga tgaaaagaag agattttcta
     1561 aaacaatcag tttttgcaag tgcaggcagt atgttgatac ctgctttttt aaaaccattt
     1621 gaactgattg caaaaagtag tttaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg
     1681 tcatcattca actttccggt ggcaatgacg gactgaacac tattgtgcct tatggtaatg
     1741 atgtatatta tcagttgcga aacactattg ccattccaca atcggaaatc attaaattaa
     1801 atgatatgca gggattgaat cctacattag ctccactaaa agaaatttac gatcagggtt
     1861 ggatgagtat catcaataat gtgggttatc cgaatccaaa ccgctctcac tttaggtcaa
     1921 tggacatctg gcaaacagca agtgattcca atcagtttct ttctacagga tggattggac
     1981 gctatttaga ttcaaactgt cagatgtgca agtatccata caatgctatt gaagtggacg
     2041 acaccttatc tttaactttg aaaggtgatt ctaaaaaagg tattgcactt caggatccgg
     2101 acaaattgtt tcgtaccacc aacacacctt tttttggcaa gttgatagaa cacgataaag
     2161 agcatcttga cgaagataat ctaggatact tgtacaaaac catgattgaa acatattcat
     2221 cggcaagcta tattaaagaa acaacaaaga attatgataa taactttgtg tatcccgaat
     2281 cgtcatttgc aaagaaatta aaaaccgttg cgcgatttat attatcagga cttgatacaa
     2341 gagtttatta tgtttctctc agtggatttg atacgcatgt aaaccaaatg gcgcaacaaa
     2401 aaaaattgtt tactcagttt tctgaaggca tgaatgcatt tgtaaaagag ttgaacaata
     2461 ataaaaaact tgaagataca ttagtgattg ccttttcgga atttggtaga agagtggcgc
     2521 aaaatgcaag ccgtggcacc gaccatggta cagcaaatgt tatgatgctg tttggcggtc
     2581 agttaaagaa gcagggaata tataatgatg ctcctgattt gaatgatctt gatgcaggag
     2641 atttaaaata taaagttgat ttcagagaga tatatgcaac agtattgaac aaatggctca
     2701 atgtggacaa taaattaatt cttcctgatt caacaggtac aatggatttt atttgacgca
     2761 agatacaatg agattagtta tcaactatag ctaatttcct gcaaattttt gctccttcat
     2821 aagttaacat agcattgtaa actccatttt gtaattgtgg taatgtgatt tgatgattgc
     2881 tgctatacaa tggcaatgta tatttaaaaa cagtttttcc aattgcatca acaatttcaa
     2941 aaacaccacc tgaagaagtt ggtgaaaaat atttaatgtt tagaatatta ttacttacag
     3001 gattgggata aaaataaaat tcctgagatg acaactcatt ataatcagtg ttgccggaat
     3061 tgttcagggt atatcctact cgtttaatgc cgaagggttc attggcaaac catacaaggt
     3121 cattatcatt atcgccatca agatcaccac cattaacaaa agtataacga taaacagaat
     3181 caataaaatg agagaagttt aatccaccta ctccatcatt tgacttgtaa tagaggcctc
     3241 tgtttacaag tggaatataa agtgcatccg gaaatgtatc attatctaaa gtgagaaatt
     3301 ttaatggtgg attttgattg ttacctgcat ggtacatcac ggattgaaaa ttactaaaac
     3361 ctcccgagcc atcatttttc aaagttatca agttgaaaga aacagctgaa tcgggataga
     3421 aaagatcaaa gtcaccatca ttatcaatgt cacaagattc taaagtgaga taaagtgcag
     3481 ttgtaaatgt ttgaggactg aaagtaaggt tatgattatt tataaaaagc ctgaatccag
     3541 cagcagaatg aactgaaaaa tcaacaaagc catcaccatt aaaatcatgc acaactaaat
     3601 tgtaaaattc actactgtct gctactttaa tcattcctga aaaagttcct gcagattgct
     3661 cataaattgc aacctctaaa tcctgacaac tgataatgtc aagatctgaa tcgttatcta
     3721 agtcaactaa ataacttctt actacgaaag catcaatact atcattgatg attatttgct
     3781 gccaggtgtt tccaccgaaa tttttaaata acttcaatcc tgcatagaca tcatcataac
     3841 tcaccaaaac atcttgtaat ccgttaccat caacatctgc acaatctaca aaatatggac
     3901 gtttcactgt gtttgaaatt gcagacatac gattaaataa aagtccattg tttttatacc
     3961 aataaacaga atcatataag tttgatacta aaatatcctt ttggttatca ttatcaaggt
     4021 caacaatatt gattg
//
LOCUS       DAORQT010000139         4027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4043_length_4027_cov_6.335347, whole genome shotgun sequence.
ACCESSION   DAORQT010000139 DAORQT010000000
VERSION     DAORQT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_4027_cov_6.335347"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PK134_05695"
     CDS             <1..677
                     /locus_tag="PK134_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38377.1"
                     /translation="DSTKKTALTTVFKQLAGNLSINKTDYESVKIEMMDTDPLQAKQM
                     ADSIIEKGNRLARKLYREKTMEAYVVAKTQFDMKENELDSADKVLTELRMKSGIIEFS
                     QQTREISRAYYTSILNKSNNVRLEEMFQKLQQYGSDYVTLNEKINYTRGVYNNYKQYV
                     ENAEKDLTKELSYSNVVTPPMIPDKKAYPVRSLIMLMFTASVVFLSFVVIIIYENSQE
                     KKPQTN"
     gene            745..2130
                     /locus_tag="PK134_05700"
     CDS             745..2130
                     /locus_tag="PK134_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB38378.1"
                     /translation="MAVFSLVNEFYWLLILPAGILIGYSALYHLDKLFLFTAFCTPLA
                     INLSKTDLGIGVSLPTEPILFGMMIIYLLSKFIDGRYEKSFIKHPITVLILIHLAWML
                     ITSFTSSMFLVSIKHFVARLWFVVIGFMLATSIFKDEKKMRQFIWFYAVGMAITIIYT
                     ITRHSINGFTQQSAHWVMTPFYNDHTEYAAVLALMLPPIFGLMLLKHKSFMYRFFAFL
                     ISGLVIVAIVLSYTRATWLSLAVALVSMLAYVFRVKTWAVVAIIISMVVGFQIFQSTV
                     LYKFGKTKEYSQNDYRGHLKSITNIKTDASNVERLNRWSCAWRMFLDKPVFGWGPGTY
                     QFQYSVYQLQFEKTYISTNLGDKGNAHSEYLGPLSEQGFMGTIIFVALAVVVLYKASR
                     VFLQTNSNNIKVLALSMQVGLITYLVHGFLNNFLDKDKASIPFWAFVAAITVMDYYHN
                     KKQLNPSTERA"
     gene            complement(2113..3339)
                     /locus_tag="PK134_05705"
     CDS             complement(2113..3339)
                     /locus_tag="PK134_05705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRB38379.1"
                     /translation="MEETTDKLAADLQSKKVQLHWLLQITKAINYNLPAEQLYQIYEN
                     VLHDHLKAGKLSLFVNENGWKQVLSYGMKEELGRLQPELFLNEISFSKSKTVATPQWL
                     EKDESVIPVYHNEMLLAYCIVGAIDPSQSISIKEVVPFIHTITNIIVVAIENKRLNKE
                     AVAQAGFRKELELAAEMQEMLFPVGLCGNEYFEIATHYLPHQQVGGDYYDFISLNKHE
                     HIFCMADVSGKGMAAALLMSNFQANLHALIKNRPVLTNLVAELNDCVSKTAKGEKFIT
                     FFIGLINFKKGEINYVNAGHNPPVFCSKNKSELLERGTTGLGMFDELPFINEGHVKIN
                     SGDSVFCYTDGITELENLKGDFYGMEALKLFIEQNMNNSSLDEFHKKLIADLDHFREG
                     NSFSDDVTILSLRSLS"
     gene            complement(3342..3761)
                     /locus_tag="PK134_05710"
     CDS             complement(3342..3761)
                     /locus_tag="PK134_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38380.1"
                     /translation="MHTCGRVIIGDYVEIGACCTVDKGVSGDTVIGSGTRMDNHVHVG
                     HDTVIGKNCLFAAQVGIAGVVTIEDDVILWGQVGVQKDLTIGKGAVVLGQSGVGKTLD
                     AGKTYFGSPTTDARLKMRELALMKRLPEVFEFLDNKE"
     gene            complement(3786..>4027)
                     /locus_tag="PK134_05715"
     CDS             complement(3786..>4027)
                     /locus_tag="PK134_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRB38381.1"
                     /translation="YNLLTKKYRPLTFSMQQVDQTAVIGEGTVLMQGSVVGAHSKVGK
                     NCLIYPGVVIYDHCEIGDGVIIHANSVIGADAFYF"
BASE COUNT         1246 a          822 c          675 g         1284 t
ORIGIN      
        1 ttgactcaac caagaaaacc gctttaacta ccgtttttaa acaacttgca ggcaatttga
       61 gtatcaataa aaccgactac gaatcagtta aaattgaaat gatggatacc gatccattgc
      121 aggctaaaca aatggccgat tctataatag aaaaaggtaa caggcttgca agaaaacttt
      181 acagagaaaa aacgatggaa gcttacgtgg ttgccaaaac acaatttgat atgaaagaaa
      241 atgagctcga ttctgccgat aaggttttaa cagagttaag aatgaagtcc ggtatcattg
      301 agttctcaca acagacacgc gaaatttcaa gagcatatta tacttcaatt ttaaataaaa
      361 gcaataatgt gcgtttggaa gaaatgtttc agaaattgca acaatacggc tcggattatg
      421 tgacactcaa cgagaaaatc aattacaccc gtggagttta taacaactac aagcagtatg
      481 tagaaaatgc tgaaaaagat ttaaccaaag aactttcata ttcaaatgtg gtaacgccac
      541 caatgattcc ggataagaaa gcctatcctg ttcgcagttt aataatgctg atgtttactg
      601 cctctgtggt attcttatct tttgtagtaa tcatcatcta cgaaaattct caggaaaaga
      661 aacctcaaac aaattaattt tatgcaagcc attcagcaaa attcgcttcg gtggttttat
      721 ggccttacag ccatatatat gctaatggca gtattcagtt tggtgaatga attttattgg
      781 ctattgattt tgcctgcggg cattttaatc gggtactctg cactctatca tcttgacaaa
      841 ttatttcttt ttactgcatt ctgcactccc ttagcgatta acctttcaaa aactgattta
      901 ggaatcggtg tcagtctgcc aacagagcct attttgtttg gcatgatgat catctatctt
      961 ctttcaaaat ttattgatgg caggtatgag aagtccttta tcaaacatcc aattaccgtt
     1021 ctcattctga ttcatcttgc atggatgctg attacaagtt tcacaagcag catgtttctt
     1081 gtttctataa aacattttgt agcaagattg tggtttgttg ttattggctt tatgcttgca
     1141 acatccatct ttaaggatga aaagaaaatg cggcagttta tatggttcta tgctgttgga
     1201 atggcaataa ccattatcta tacaatcacc cgtcacagca taaatggatt tacacagcaa
     1261 tctgcacact gggtgatgac acctttttat aatgaccata cggaatatgc tgctgtactt
     1321 gctttgatgc tgcctccgat ttttgggttg atgcttttga aacataaatc ttttatgtac
     1381 cgcttttttg catttctgat ttcaggattg gtaattgttg caatagtatt atcatacact
     1441 cgtgccacct ggctcagtct ggctgtggct cttgtctcta tgcttgctta tgttttcaga
     1501 gtaaagacat gggctgtagt agctataata atttcaatgg ttgtgggttt tcagattttt
     1561 caatcaacag tgttatacaa attcggaaaa acaaaggagt attcacagaa tgactaccgc
     1621 gggcatttaa agtctattac caatataaag actgatgcat caaatgtgga gcgtcttaac
     1681 cgttggagtt gtgcctggcg aatgttctta gataaacctg ttttcggatg gggaccgggc
     1741 acctatcagt ttcagtattc tgtttatcaa ctacagtttg aaaaaaccta catcagcacc
     1801 aaccttggcg ataaaggaaa tgcacatagc gaatatctag gcccattaag cgaacaaggt
     1861 tttatgggaa ctataatttt tgttgcatta gctgttgtgg ttctttacaa agcatcaaga
     1921 gtctttttgc aaacgaattc taacaacatc aaggtgcttg cattatccat gcaggttggt
     1981 ttaattacct atcttgttca tggattttta aacaattttt tagataagga caaagcctca
     2041 attccatttt gggcatttgt agctgctatt accgtaatgg actattatca caataaaaaa
     2101 caactaaacc catcaactga gcgagcgtaa tgataagatg gtaacatcat cgctaaaact
     2161 attgccctct ctgaaatgat ccaaatctgc aatcagtttt ttatgaaatt catcaaggct
     2221 tgaattgttc atattctgct ctataaataa ttttaacgcc tccataccat aaaaatcacc
     2281 cttaaggttt tcgagctctg taataccatc ggtataacaa aaaacagaat caccactatt
     2341 tatttttaca tgaccttcgt ttataaatgg caactcatca aacatgccta aacctgttgt
     2401 gcctctttct aaaagttcag acttgttttt actacaaaat acaggaggat tatgaccggc
     2461 attaacataa ttaatttcac cttttttgaa atttatcagt ccgataaaaa aggttataaa
     2521 cttttcacct tttgcagtct tgctcacaca gtcatttaat tcagcaacta agtttgtaag
     2581 tactggtctg ttttttatta atgcatgcag gttagcctgg aaattagaca tcagcaatgc
     2641 agctgccatt ccttttcctg aaacatctgc catgcaaaaa atatgctcgt gtttgttgag
     2701 agaaataaaa tcataataat cacctcccac ctgttgatgt ggaagataat gtgttgctat
     2761 ctcgaaatat tcatttccac ataagccaac gggaaataac atctcctgca tttctgctgc
     2821 cagttcaagt tctttgcgaa atcctgcctg cgcaaccgct tctttattta aacgtttatt
     2881 ttctattgct accacaataa tatttgtaat ggtatgaatg aatggaacaa cttcttttat
     2941 tgaaattgac tgactcgggt caattgcacc aacaatgcaa taagccaata acatttcatt
     3001 gtgataaacc ggtataaccg attcatcttt ttcaagccac tgtggtgtgg ctactgtctt
     3061 tgatttagaa aatgatattt cattgagaaa caattccggt tgcaaacgac cgagttcttc
     3121 tttcatgcca taggacagca cctgcttcca accattctca ttgacaaaca aagaaagttt
     3181 tccagccttt aaatgatcat gcaaaacatt ttcataaatc tgatacaact gttcagcagg
     3241 caggttgtaa ttaattgctt ttgtaatctg caaaagccaa tgcaactgta ctttctttga
     3301 ctgaaggtcg gcagcaagtt tatcggtggt ttcttccatc actattcttt gttatccaaa
     3361 aactcaaaca cttcaggcaa tcgtttcatc aatgcaagtt cacgcatttt taaacgggca
     3421 tcggttgtgg gactgccgaa gtaagtctta cctgcatcta atgttttacc aactcctgac
     3481 tggccgagca ccactgctcc tttgccaata gtcaaatctt tctgaacacc aacctgtccc
     3541 cataaaataa catcatcttc aatagtgaca acaccggcta tgcccacctg tgcagcaaac
     3601 aaacagttct tacctatcac cgtatcatgg cctacatgca catgattgtc cattcttgtt
     3661 ccactgccaa taacagtatc gcctgacacg cctttatcaa ctgtacaaca tgctccgatc
     3721 tcaacataat caccaataat tacacgacca caagtatgca ttttatcaaa tccttcgggg
     3781 cgtctttaaa agtaaaaagc atcagcacca ataacactgt tggcatgaat gattacacca
     3841 tcacctattt cgcaatgatc ataaataact acaccgggat atatcagaca atttttaccc
     3901 actttactat gagcgccaac tacacttccc tgcatcaaca cagttccttc accaatcaca
     3961 gcagtttgat caacctgttg catactaaaa gttaatggtc tgtatttttt tgtcaacaga
     4021 ttataat
//
LOCUS       DAORQT010000140         3992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4094_length_3992_cov_10.407417, whole genome shotgun sequence.
ACCESSION   DAORQT010000140 DAORQT010000000
VERSION     DAORQT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4094_length_3992_cov_10.407417"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK134_05720"
     CDS             <1..779
                     /locus_tag="PK134_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRB38382.1"
                     /translation="DNEGILWFGTADGISCFDGKKFFNIPITTITNSNSYRKTTTNNF
                     GVPFPQSNFVQGFLLDKTGTLWIGTGNGVYRYNGKTYTQFANNDGIVNNTGFQINGIE
                     TFLQDKNGNVWFGGRGSEGLFRFDGKIVSHFKLDDKMWVRPLLEDSKGNLWFGSPRHN
                     MIYCYEGKEFLPFGKNEIKDWVFYMTEDREGNLWFSNGLKGGVTFYDGKTNKNLTKND
                     GLCYETVWCIASDKDGNVWFINRDESLCRYDGKSFMSLSK"
     gene            complement(921..1628)
                     /locus_tag="PK134_05725"
     CDS             complement(921..1628)
                     /locus_tag="PK134_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRB38383.1"
                     /translation="MKRFLLIALVLLGQQAYAQKKIFKATNTTPSNQGEGLANNDIIS
                     GLREALKVGTNNSTASASKLDGFYKNPMIKIPFPREIRDAESTLRSMGMDKEVDKFVK
                     TLNRAAEDAAKSAAPIFINSIQHITINDGLTILRGGNDAATQFLKTTSNAALIAAFKP
                     IVQNSLNKVKITQYWNPLMSTYNKVPFVKKVNPDLNQYVTDKAIEGLFKLIAAEELKI
                     RKDPAARINDILKSVFG"
     gene            1759..3003
                     /locus_tag="PK134_05730"
     CDS             1759..3003
                     /locus_tag="PK134_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRB38384.1"
                     /translation="MMTNVELINIGDELLIGQIVNTNATWIGQQLSLSGYWLKQITTI
                     GDNEQEIVDALNAAMSRAQIILITGGLGPTKDDLTKDVLCKYFNTQLVFNEQAYSDIE
                     NIFHARGKAVTETNKRQAELPAACIPIYNKKGTAPGMWFNHGEQVFVSMPGVPFEMQA
                     MMQNDVLPKLRQLYESMPLVHKTILTQGIGESDLSDRIAAWENDLPAHLKLAYLPAAG
                     MVRLRLTGKGVEHMEQDMNLRIDKLKELIAPYIFGYDDDTIQSVVGKLLTEKQKTLSV
                     AESCTGGFLAHLITSIPGSSTYFMGGTVSYSNAAKMSQLHVSEQSLKEYGAVSEAVAI
                     EMAIGARKTYNTDFALSTTGIAGPGGGSLEKPIGTVWIGVATHDACFAKKFMLGTNRL
                     RIIQVAADTALNMLRKKINEHN"
     gene            3060..3131
                     /locus_tag="PK134_05735"
     tRNA            3060..3131
                     /locus_tag="PK134_05735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3092..3094,aa:Met,seq:cat)
     gene            3317..3736
                     /locus_tag="PK134_05740"
     CDS             3317..3736
                     /locus_tag="PK134_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRB38385.1"
                     /translation="MTHLISIKNRMAIKEYTLLFFRIGVSLLMLYGHGYPKVQKLFSG
                     TEIQFADPFGLGMKLSFYLVVFAEVICSIFIILGLKTRMAASVLVFNMLVAVFVMATN
                     GEVPELALMYFISYVLILVFGGGKYSIDFNLNSKKKR"
BASE COUNT         1255 a          625 c          820 g         1292 t
ORIGIN      
        1 aagataatga aggaatactt tggtttggca cagcagacgg aatttcttgt ttcgatggga
       61 aaaagttctt taatatacct ataacaacta ttactaatag taattcatac cgtaaaacca
      121 caacaaacaa ctttggtgta ccatttccgc agagcaattt tgtacaagga tttttactcg
      181 ataaaacagg aacactttgg attggaaccg gaaatggagt gtatcgttac aatggcaaaa
      241 catacactca atttgccaat aatgatggta ttgtaaacaa taccggtttt caaattaatg
      301 gtattgaaac tttcttgcaa gacaaaaacg gtaatgtttg gtttggtggt cgcggcagtg
      361 aagggctttt tcgttttgat ggcaaaatag taagtcattt caaactagac gacaaaatgt
      421 gggtcagacc acttttagaa gacagtaaag gaaatctttg gtttggctct cctcgtcata
      481 acatgatata ctgctatgaa ggaaaagaat ttcttccatt tggcaagaac gaaattaaag
      541 attgggtttt ttatatgaca gaagatagag aaggaaacct ttggtttagt aatggtttga
      601 agggaggtgt gactttctat gacgggaaaa caaataaaaa ccttactaaa aatgacggtt
      661 tgtgttatga aaccgtttgg tgcattgcat cagacaagga cggtaatgtt tggtttataa
      721 atagggatga aagtttatgt cgttatgacg gaaaatcatt tatgagttta tcaaagtaac
      781 aattggaggc aggtacattt aagtaaagat ggagaacaaa cacttcagtt taatgtctat
      841 gcaatgtaca agcccgatta ctgtcgggct tgtgcattga tgtaaaatta caatacttcg
      901 tcaataggca aaccgttgat ttacccaaaa acacttttaa gaatatcgtt gattcgtgca
      961 gcaggatctt ttctgatttt cagctcttct gcagcaatga gtttaaaaag accttcaata
     1021 gctttatcag taacgtattg attcaggtct ggatttactt tttttacaaa tggcacttta
     1081 ttgtaagttg acatcaatgg attccagtac tgcgttatct ttactttgtt aagcgaattt
     1141 tggactattg gcttgaaggc tgctatcagc gctgcatttg aagtggtttt taaaaattgt
     1201 gttgctgcat cattaccacc tcttaatatg gtaagtccat cattaatagt tatatgctga
     1261 atagaattga tgaaaattgg tgctgccgat tttgctgcat cttctgctgc acggtttaat
     1321 gttttaacaa atttgtcaac ttctttatcc atgcccatgc tgcgtaaagt gctttctgca
     1381 tcgcgtattt cacgagggaa aggtattttt atcatcgggt tcttataaaa gccatcaagc
     1441 tttgatgctg aagcagtaga gttgtttgtg cctactttaa gtgcttctct gaggccactg
     1501 atgatgtcgt tgttagccaa accttcacct tgatttgaag gggtagtatt tgtagctttg
     1561 aaaatctttt tctgtgcata agcttgctgt cctaataaca caagcgcaat taaaagaaac
     1621 cgtttcataa tctatttttt tagaattttg tttttcaatt caaacatatt gccattatat
     1681 gtctgcaatc aatattgttt gtttacttaa tctgaaatta atgcagctaa aatacttaat
     1741 taatttaaag ttcactttat gatgacaaat gtagagctaa taaatattgg tgacgaacta
     1801 ctcattggac agattgtaaa taccaatgca acatggattg gacagcaact aagcctttcc
     1861 ggttattggc tgaaacaaat taccaccatt ggtgataatg agcaggaaat tgttgatgca
     1921 ttaaatgctg caatgagtcg tgcacaaatt attctcatta caggtggact agggccaaca
     1981 aaagatgatt taacaaagga tgtgttatgt aaatatttta atacacaact agtgtttaac
     2041 gaacaggcat atagtgatat tgaaaatatt tttcatgcaa gaggtaaggc agttacagaa
     2101 acaaacaaac ggcaggccga attgcctgct gcatgtattc caatttacaa taagaaagga
     2161 actgctccgg gaatgtggtt caatcatggc gagcaggttt ttgtgagcat gccgggtgtg
     2221 ccattcgaga tgcaggcaat gatgcagaat gatgtacttc ctaaattaag gcagctatat
     2281 gagtcaatgc ctttagtaca taaaacaatt cttacgcaag gtataggaga gagcgatttg
     2341 tcagatagga ttgcagcatg ggaaaacgac ttgcctgctc atttaaaatt agcctatctg
     2401 cctgctgcag gaatggttcg tttacgactt acaggtaaag gtgttgaaca catggagcaa
     2461 gacatgaacc tgaggattga taaactcaaa gaattaattg cgccttatat ttttggttat
     2521 gatgatgaca caattcaatc tgttgtagga aaacttttaa ctgagaaaca aaaaactctt
     2581 tcagttgcag aaagttgtac tggaggattt ttggcacatc ttattacttc aatccccggt
     2641 agttcaacat actttatggg cggtacagtg agttacagta atgcagcaaa aatgagtcag
     2701 ttgcatgtca gcgaacaatc attgaaggaa tatggtgccg tcagtgaagc ggttgcaata
     2761 gaaatggcaa taggtgcaag aaaaacttat aatacagatt ttgcattgag tacaacaggc
     2821 attgcagggc ctggtggagg aagtttggaa aaaccgattg gcactgtatg gattggagtt
     2881 gctacacacg atgcttgttt tgctaaaaag tttatgttgg gtacaaaccg tctgcgaatc
     2941 attcaggtag ctgcggatac tgcattaaac atgctcagaa agaaaataaa tgagcataat
     3001 tgaggaatat atttgcagat aaggacaaag tgattacttt tgcaccctca aaataacaat
     3061 gcggggtgga gcaggggtag ctcgttgggc tcataaccca aaggtcgttg gttcgaatcc
     3121 agcccccgct actaaaagat taagctgcac agagtgcagc ttttttattt ccttttactt
     3181 tattgatata tagattttca aaacgcaagt tcattcttca atctgagttg aaatattgcc
     3241 gattgtgtct gattttgtgg tattgattga aatccaagga aaattttatt tttggagaaa
     3301 caaaattagt ataggcatga cacatttaat tagcattaaa aatcgaatgg ccataaagga
     3361 atatactttg ctgtttttca gaataggagt ttcattgctg atgctttatg gtcacggcta
     3421 tcctaaagta cagaaattat ttagtggaac agaaattcaa tttgccgatc cgtttgggct
     3481 tggcatgaag ctttcttttt atctggtagt ttttgctgaa gtgatttgtt ctatatttat
     3541 catactcgga ctaaaaacac gtatggctgc atctgtattg gtttttaata tgcttgttgc
     3601 agttttcgtc atggctacta atggagaagt tcctgaattg gcactcatgt attttatctc
     3661 ttatgtactg attttagttt ttggcggagg taaatactct attgatttta atcttaattc
     3721 aaagaagaaa aggtgagcat agttgatttt aatttatttt taaatgttga ataaaaattt
     3781 caaaatcatg aaaaagataa gttttattat ttttttggct tgtgcaatta gtatgcaatt
     3841 acaagctcag gtaattaccc aacttggtca atggagtaat aatgccaatt ttgcagttga
     3901 atattatcag gataaagtta tcaccagcac cacttcaggc atcaaattta ttgatgtgtc
     3961 gaacccatcg aatccagttc ctactacaac ct
//
LOCUS       DAORQT010000141         3992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4096_length_3992_cov_6.262383, whole genome shotgun sequence.
ACCESSION   DAORQT010000141 DAORQT010000000
VERSION     DAORQT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4096_length_3992_cov_6.262383"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PK134_05745"
     CDS             complement(<1..709)
                     /locus_tag="PK134_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38386.1"
                     /translation="MKKLLLLLLIGLTVKCAAQDYAGVRVGDTAHYYHQTNYYFPLGK
                     LKAIAIDSVAVNANETELFNTFLIHGQDPLCIKYDRENWTGKKITRTINGIEKYITGN
                     NDTIVVDPITQVGSSWVFCNLANNTHFDATVLSVQPESFLNISDSVISFSLQLKDNSS
                     QNIAHPFNGKTLKLSKQYGWIHTINFLDFPTDTALLTITGTTQSNLGYQNLIADSIFN
                     FNIGDTFQYHDYGQSAIN"
     gene            887..1573
                     /locus_tag="PK134_05750"
     CDS             887..1573
                     /locus_tag="PK134_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB38387.1"
                     /translation="MNNRSYKILIVDDEPDILEFIQYNLRKEGYFVYTAKNGEEAVAL
                     AKQEVPHLIVMDIMMPVMDGVEACRIMRTIPALNKTIITFLTARSEDYSQIAGFDVGA
                     DDYIQKPIRLPVLMSRIKALLRRASELPVADASVIEVGGLKIDRMHYLIYQNGQTFEL
                     PRKEFELISLLASKPGKVFSREDILSRVWGNDIVVGNRTIDVHIRKIREKLGDQVIKT
                     IKGIGYKIEP"
     gene            complement(1596..1940)
                     /locus_tag="PK134_05755"
     CDS             complement(1596..1940)
                     /locus_tag="PK134_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRB38388.1"
                     /translation="MKKLIAGAVMLLFFSFSLMAQQNKKAEINIATSAECTMCKKALE
                     TALLHQHGVKFAKLDLNTRVVAVKYNGEKVTPETLRQVISNAGYDADSVKANPEAFEK
                     LPDCCKNGALHH"
     gene            complement(1950..>3992)
                     /locus_tag="PK134_05760"
     CDS             complement(1950..>3992)
                     /locus_tag="PK134_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRB38389.1"
                     /translation="DGKFQIQYTGTTPLTIISQFVGFKTDTMTLNAIPDNDLKIRLKK
                     SIDLKEVEIKSRRESTSISTITPINTTTITQHELLKAACCNLSESFETNPTIDVNYSD
                     AVTGAKQIQMLGLDGIYSQIQFQNLPLIFGLSASHGLAFTPGPWIESIQINKGIGSVI
                     NGFEAMTGQINIELKKPQNADRFFINGYLNNEGREELNLQLAQRFKNHISTVFLAHGS
                     MNHLKVDRDKDGFLDQPLTKQVNAMNVWHITVPNKLEGEIGWRALYENRLGGQKGFNY
                     DDDFGKSTLYGVGIENKMFEVYTKTGTINPAKPYRSIALLTLSRLHLQDNYYGNKTYL
                     GDQRSFNANTIYQTIIHDTRHYIRSGLSFRYNHYSEKYNSVGTYTNEIIPGAYAEYSF
                     NNDLNWAVVAGLRADIHNTYGFFLTPRLHIKYNIDQLTAIRLSAGKGWRTARVYAENT
                     SVFTSSRALFIDDLKAEEAWNTGINFTKKMQWFGHDASFNADYYYTWFKNQVVIDMEN
                     IHQLRFYNLDGKSFSHYLQSELNIEPISNLLLRFGYKYNLVKQTFSGELKSKPFTPRD
                     KALMNAEYKIKKGKYSIDATLKYTGKGRIPGGGININGYELKSNSESFMTLNSQATWR
                     TKHADFYLGCENITDYRQKHAIIAADEPFGQNFDASMIWGPLMGRTFYVGFRYTIK"
BASE COUNT         1213 a          670 c          735 g         1374 t
ORIGIN      
        1 gattaattgc agattgtcca taatcatgat attgaaaagt atcaccaata ttaaaattga
       61 agatgctgtc agcaattaga ttttgataac ctaaatttga ctgtgtagtc cccgttattg
      121 tcaataaggc tgtatctgtt ggaaaatcaa ggaagtttat tgtatgtatc caaccatatt
      181 gtttactcag ctttaacgtt tttccgttga aaggatgtgc aatattttga ctggaattat
      241 cttttaattg taatgagaaa cttattacac tatcgctgat attaagaaag gattcaggtt
      301 gtacagaaag aaccgttgca tcaaaatgcg tgttgtttgc taaattacaa aaaacccatg
      361 atgatccaac ttgtgttata gggtctacaa caattgtgtc attattaccg gttatatatt
      421 tttcaatgcc gttaatggtt cttgtaattt ttttgccggt ccagttttct cgatcatatt
      481 ttatacacaa tggatcttga ccatgaataa gaaaagtgtt gaaaagctct gtttcatttg
      541 cattaacagc aacgctgtca atcgcaatag cctttaattt acctagtggg aaatagtaat
      601 tagtttggtg ataataatgt gcagtatcac cgacacgaac acctgcataa tcttgtgctg
      661 cacatttaac tgttaatcca atcaaaagga gcagtagtaa ttttttcatg agaatatatt
      721 tatgtaaata tacagaataa ttcaaacaag caaattggtt ggtcattttt gaatggaaca
      781 ggaatttaaa gaatgtcatt ctaaaacaac gcaaaagttt gagaaaattt aatttataga
      841 ttcaaaattc aatgcagtta actttctttg cattagttat agaaaaatga ataatcggtc
      901 ttataaaata ttgattgttg atgatgaacc ggatatcctt gagtttattc aatataatct
      961 aaggaaggaa ggttattttg tttacacagc taagaatggg gaagaagcag ttgctttggc
     1021 taagcaggaa gtgcctcatt tgattgtaat ggacattatg atgcctgtga tggatggtgt
     1081 tgaggcctgt cgtataatgc gcacaatacc tgctcttaat aaaactatta taacattttt
     1141 aacagcgcga agtgaagatt attctcagat agcggggttt gatgtaggtg ctgatgatta
     1201 catacagaaa cctattcggt tacctgtgtt gatgagtcgc attaaagcgt tgctgcgcag
     1261 agcgagtgag ttgcctgtgg cagacgcatc agtaattgag gtaggcggat taaaaattga
     1321 tcgcatgcat tatcttattt atcaaaacgg ccaaactttt gaattacctc gaaaggagtt
     1381 tgaattaatt tctttactgg catccaagcc cggcaaagtt ttttcgagag aagatatact
     1441 cagccgggtg tggggtaatg atattgtagt aggcaataga actattgatg ttcacattag
     1501 aaaaataaga gaaaagttag gtgatcaggt aattaaaacc attaaaggaa ttggttataa
     1561 aattgaaccc tgaaagattc tcttttcact aatgcctaat gatgcaatgc gccattcttg
     1621 caacagtcag gcagtttttc aaatgcctca ggattggctt ttacactatc tgcatcataa
     1681 cctgcattac tgattacctg gcgaagtgtt tccggtgtaa ctttttcgcc attgtattta
     1741 acagcaacca ctcttgtatt aaggtcgagc tttgcgaatt ttacaccatg ttgatgcagt
     1801 aaggcagtct ctaaggcttt cttgcacatg gtacattctg ccgaagtagc aatgtttatt
     1861 tcagcttttt tattttgttg tgccatcagt gagaaactga agaacaataa cataacggca
     1921 cctgcaatta actttttcat atttattgtt tatttaattg tataacgaaa tcctacataa
     1981 aatgtgcgac ccattaatgg cccccatatc attgaagcat caaaattctg accaaaaggt
     2041 tcatcagcgg caataatagc atgcttttgt ctatagtctg taatattctc gcaacctaaa
     2101 taaaaatctg catgtttggt tctccaggtt gcttgcgaat tcaatgtcat gaaactttcc
     2161 gaatttgatt ttaattcata accattgatg ttgatacctc cacccggaat tctgcctttg
     2221 ccggtgtatt ttaatgttgc atcaatactg tatttccctt ttttaatttt atattctgca
     2281 ttcattaatg ctttgtcacg aggagtaaat ggtttgcttt ttaattcacc tgaaaaagtt
     2341 tgcttaacta agttgtattt ataaccaaat ctcaacagca gattacttat tggctcaata
     2401 tttaattcgc tctgcagata atgtgagaaa gatttcccat ctaaattata gaaacgaagt
     2461 tgatgaatat tttccatatc aattactacc tgatttttaa accatgtata ataatagtcg
     2521 gcattgaatg atgcatcgtg cccaaaccat tgcattttct ttgtgaaatt aattcctgta
     2581 ttccatgctt cttcagcttt tagatcatca ataaataatg ctcttgaaga agtaaacaca
     2641 gaagtgttct ctgcataaac tctggcagtg cgccagccct taccggcaga taaacgaatt
     2701 gcagttaatt gatcaatatt gtattttata tgcaaacgtg gagtcaggaa gaagccataa
     2761 gtattgtgta tatcggctct taatcctgca acgacagccc agtttaaatc gttgttgaat
     2821 gaatactctg cataagcacc gggaatgatt tcattggtat aggtgccgac tgaattatat
     2881 ttctcggaat aatgattata acgaaaactc aaaccagagc ggatataatg tcttgtatcg
     2941 tgaatgatag tctgataaat ggtgttggca ttaaaactac gttgatcgcc taaatatgtt
     3001 ttgttgccgt aataattatc ttgcaagtgt aaacgtgaaa gcgttaataa tgcaatactt
     3061 ctgtatggtt tggcaggatt tattgttccg gtttttgtat aaacttcaaa cattttgttt
     3121 tcaataccta caccatataa agttgactta ccgaaatcat cgtcataatt aaatcctttt
     3181 tgtccaccta aacggttttc atataaggca cgccagccaa tttctccttc tagtttattg
     3241 ggaacagtaa tgtgccaaac gttcatagcg ttgacctgtt ttgttaaagg ctgatctaaa
     3301 aaaccgtctt tatctctgtc cactttaaga tgattcatgc tgccatgtgc aagaaaaacc
     3361 gtgctgatat gatttttaaa tctttgtgca agttgcaggt taagttcttc acgaccttca
     3421 ttattcagat aaccattaat aaaaaagcga tcggcatttt gtggtttttt cagctctata
     3481 tttatttggc ctgtcattgc ttcaaaacca tttattaccg agccaattcc tttattgatt
     3541 tgtattgatt caatccatgg gccgggagta aaagccaagc catgtgatgc agacaaacca
     3601 aaaatcaaag gtaagttctg aaactgtatt tgcgaataga taccatcaag cccaagcatt
     3661 tgaatttgtt tggcacctgt gactgcatcg ctgtagttga catcaattgt tggattggtt
     3721 tcgaaactct cacttaaatt acaacatgca gctttcagca attcatgttg tgtgattgtt
     3781 gtcgtattta taggtgtgat ggttgaaatt gatgtacttt ctctgcgcga ttttatttcc
     3841 acttctttta agtcaataga ctttttcaga cgaattttta aatcgttatc aggtattgca
     3901 tttaatgtca ttgtatcggt tttaaaacct acaaactgac tgatgatggt aagtggtgtt
     3961 gtgcctgtgt actgtatttg aaattttccg tc
//
LOCUS       DAORQT010000142         3965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4151_length_3965_cov_9.781586, whole genome shotgun sequence.
ACCESSION   DAORQT010000142 DAORQT010000000
VERSION     DAORQT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4151_length_3965_cov_9.781586"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK134_05765"
     CDS             <1..814
                     /locus_tag="PK134_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB38390.1"
                     /translation="KSTVRLACNVIESTFFNDVPVETSTLEFQQLDISITELMQRIKA
                     AFMTEKEFISNVSHELQTPISILKTRFENMLMDEKIDDDVADKLDDSIRKLNRMSKII
                     KSLLMISKIDNEQFLKNETVNIKALLQEIAEDFQEHLSTKNIRLYMQHLDEFEIQNAN
                     RTLLYTLFSNIITNAIKYNVENGQIDISATKQSNVFVVAIRDTGVGIAKENLTHIFDR
                     FKKFNKSTEPSFGLGLAIVKTISDFHRLNLHVESEANKGTTFRVEFPIEPFT"
     gene            862..1155
                     /locus_tag="PK134_05770"
     CDS             862..1155
                     /locus_tag="PK134_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38391.1"
                     /translation="MNIYNLVLFSSCLFLTQSALGQDQARPIKTGGRPAEVREINNTP
                     VIMIHKSNDVHDKGNSNMQQRKSPRCIKKVGACTEGGDRKKELKEPVRINENQ"
     gene            complement(1149..1514)
                     /locus_tag="PK134_05775"
     CDS             complement(1149..1514)
                     /locus_tag="PK134_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRB38392.1"
                     /translation="MTQKKTLVLGASTNPARYSNKAILKLRYYKHPVVAIGGKPEKVE
                     DITIETGNVKFKDIDTVTMYLNAERQKSLEDYILSLKPKRIIFNPGAENESLKTKAEK
                     QGIECVEACTLVMLTIGAY"
     gene            complement(1515..2777)
                     /locus_tag="PK134_05780"
     CDS             complement(1515..2777)
                     /locus_tag="PK134_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB38393.1"
                     /translation="MSEHKETKNPLNIIVIVAALGYFVDIYDLILFGIVRTPSLNGLG
                     ITDPDLVTAYGLHLLSYQMIGMLIGGLLWGIMGDKKGRVSVLFGSIIMYSIANIANAY
                     VKEIADYTSSSIINTYTVLRFIAGVGLAGELGAGITLVNETMTKEHRGYGTMIVVSFG
                     VLGAVFANILGHFVTWQSSYIIGGVMGLVLLFLRIGVYESGMFKNLQVHSRERGNLMM
                     LFNNRKRFLTYLSSIAVGVPIWYMIGMLVVFSPEIAKMNGITGVNGGDAIMYFYLATC
                     IGDFLSAYWSQKLKSRKKVLALYLLLAICITPVYLYMMSHATIAVFYFICALLGFASG
                     YWAVFVTTASEQFGTNIRATVTTTVPNMVRGSLAILTLLLSYFRDSLQMSMIDAMFLV
                     GIISIAGAFLSLFGIKESYAKDLNYIES"
     gene            complement(2774..>3367)
                     /gene="prmA"
                     /locus_tag="PK134_05785"
     CDS             complement(2774..>3367)
                     /gene="prmA"
                     /locus_tag="PK134_05785"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRB38394.1"
                     /translation="KNFPPIRVKNIYVRAPFHPDATESDLEIIIQPKMAFGTGHHATT
                     AMMMELMMEANIADKRVLDMGCGSGILAILASKLGCSSATAIDNDPNCVLNSIENLEN
                     NKIRNVEVLQGDASSLIDLKFEVILANINRNILLDDIASYNNALPDGGQLLVSGFLTE
                     DVELITQSFGKYGLKLSSMKQNQNWVAIHFFKTHSAK"
     assembly_gap    3368..3392
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3393..3628)
                     /locus_tag="PK134_05790"
     CDS             complement(<3393..3628)
                     /locus_tag="PK134_05790"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRB38395.1"
                     /translation="MNTYIAIHCKVEPVQPFSEILIAQLAEIGFEMFEEKEDGFDGYI
                     RKENFNESLLETIEFLQPNDFCSVHWQLEEIESQ"
     gene            complement(3703..>3965)
                     /locus_tag="PK134_05795"
     CDS             complement(3703..>3965)
                     /locus_tag="PK134_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRB38396.1"
                     /translation="LILDDCLSALDTGTEEQLLQNLLPYFQHRITLILGHRISSVKHA
                     DEIIVLHQGKILERGTHLSLLQQNGFYSKLVTEQIKKQEVKS"
BASE COUNT         1300 a          740 c          670 g         1230 t
ORIGIN      
        1 caaaagtaca gtcagactcg cgtgcaatgt cattgagtcc acctttttca atgatgttcc
       61 tgtagagact tcaacgcttg agtttcaaca gttagatatt agcattacag agttgatgca
      121 acgtattaaa gcggcattta tgactgagaa agagtttatc tcaaatgtat cgcatgagtt
      181 acagacacca atttctattc tgaaaactag atttgaaaat atgctcatgg atgaaaaaat
      241 tgatgatgat gtagctgata aactcgatga ttcaatcaga aagctgaaca ggatgagtaa
      301 aatcatcaaa tcattgctga tgatttcaaa aattgataat gaacagtttt tgaaaaatga
      361 aaccgtgaat ataaaagcac tacttcagga aattgcagaa gattttcaag agcatttaag
      421 tactaaaaat atcagacttt acatgcagca tttggatgag tttgaaatcc agaatgcaaa
      481 tagaactttg ctctatacac tttttagtaa tatcattact aatgctatta agtataatgt
      541 agaaaatgga cagattgata ttagtgctac caaacagagt aatgtttttg tggttgcaat
      601 cagagataca ggcgttggta tagcaaaaga gaatctcaca catattttcg ataggtttaa
      661 gaaatttaat aagtcaacag agccaagttt tggattagga ttagctattg ttaaaaccat
      721 ttctgatttt catcgtttaa atcttcatgt agaatcggaa gcaaacaaag gcaccacatt
      781 cagagttgaa tttcctatag aacctttcac ttgaatttca ccggcaatgt cgtaaatttg
      841 ctttcacatt acaacaacaa aatgaatatt tacaatctgg ttttattttc atcctgttta
      901 tttctgactc agagtgcatt gggtcaggat caggctcgcc caataaaaac cggtggacgt
      961 cctgcagagg ttagagagat taacaacact cctgtaatca tgattcataa atcgaatgat
     1021 gtacatgata aaggaaattc aaacatgcaa caaagaaaaa gtccacgttg tattaaaaaa
     1081 gttggtgctt gcaccgaagg cggtgacagg aagaaagaac tgaaagagcc tgtaaggatt
     1141 aacgaaaatc agtaagcccc aatggtgagc atcaccaatg tgcatgcttc cacacattca
     1201 ataccttgct tttctgcttt agttttaagt gattcgtttt ctgctcctgg attaaaaatt
     1261 attctttttg gcttaagaga taaaatgtag tcttccaatg atttttgccg ctctgcatta
     1321 agatacattg taacagtatc aatatcttta aattttacat ttcccgtttc aatagtaata
     1381 tcttctactt tttcgggctt gccgcctatt gctaccacgg gatgtttgta gtagcgcagt
     1441 tttaaaatgg ctttgtttga atatcgtgcg ggatttgttg aagcacccag cactaatgtt
     1501 ttcttttgtg tcatttaact ttcaatataa ttcaagtctt tagcataact ttcttttatg
     1561 ccaaacaaag ataagaaagc tcctgctatt gaaattatac ctactaaaaa catagcatca
     1621 atcattgaca tttgcagact atcacggaaa taggacagaa gtaaagttaa aattgccaac
     1681 gacccacgta ccatattggg tactgttgtt gttactgtag ctctgatgtt ggttccaaac
     1741 tgttctgatg ctgttgtaac gaaaactgcc caatagcccg atgcaaatcc taacagagca
     1801 catataaaat aaaataccgc aatagtagca tggctcatca tgtagaggta aacaggggta
     1861 atacaaatag caagtaagag atacaacgct aatacttttt ttctgctttt taatttctga
     1921 ctccagtatg cacttaaaaa atcgccaata caggttgcta aatagaaata cataatagca
     1981 tcaccaccat taacgcctgt gatgccattc atttttgcaa tttcaggtga aaaaacaact
     2041 aacattccaa tcatgtacca gattggtact cccacggcaa tactactcag ataggttaag
     2101 aatctttttc tgttattgaa tagcatcatc agattaccgc gttcacggct atgcacctga
     2161 agatttttaa acatgccact ttcataaaca ccaattctca gaaacagtaa aaccaatccc
     2221 attacaccgc caataatgta tgatgattgc catgttacaa agtgtccgag aatatttgca
     2281 aaaactgctc ccaaaacacc aaatgaaact acaatcatag tcccataacc acgatgttct
     2341 ttagtcatgg tttcatttac caaagttatt cctgctccta gttctcctgc taatcctaca
     2401 cctgcaataa accgtaaaac tgtataggta tttattattg atgaagaagt ataatctgca
     2461 atctctttta cataagcgtt agcaatattg gcaatagagt acatgataat agaaccgaat
     2521 aaaacggaaa cacgaccttt tttatcaccc ataatccccc aaagcaatcc accaatgagc
     2581 ataccgatca tctgataact taaaaggtgt aagccatacg ctgttactaa atcaggatcg
     2641 gtaatgccca ggccgttaag gcttggcgta cgtacaatac cgaatagaat caaatcatag
     2701 atgtcaacaa aatagcctaa tgcggctaca atgacaatga tatttaacgg atttttagtt
     2761 tccttatgtt cgctcattta gcggaatggg ttttgaaaaa atgtatggct acccagttct
     2821 gattttgctt catgcttgaa agtttcaaac catattttcc aaatgattgt gtgataagtt
     2881 caacatcttc tgtcagaaaa ccactcacca acaactgacc tccatcaggc aatgcattat
     2941 tgtaagatgc aatatcgtcc aataaaatat tccggtttat attggcaagt ataacttcaa
     3001 attttaaatc tataagtgat gatgcatcac cctgcagcac ttcaacgttt ctgattttat
     3061 tattttcaag attctcaata ctgttaagca cacagtttgg gtcgttatca atggcagttg
     3121 cagaactaca accaagtttg gatgcaagta ttgccagaat gcctgagcca caccccatat
     3181 caagaacacg tttatcagca atattagctt ccatcatcag ttccatcatc atagcagttg
     3241 tggcatgatg ccctgtgcca aatgccattt taggctgaat gataatttca agatcactct
     3301 ctgttgcatc aggatgaaat ggagctcgca cataaatatt tttaactctt attggcggaa
     3361 aatttttnnn nnnnnnnnnn nnnnnnnnnn nnttttgact ttctatttct tctaactgcc
     3421 agtgcacaga gcaaaaatcg tttggctgca aaaattctat tgtttctaat aaggactcat
     3481 taaaattttc tttcctgata tatccatcaa agccatcttc tttttcttca aacatttcaa
     3541 aaccgatttc agcgagttgg gctatcagta tttcactgaa tggctgaaca ggttctactt
     3601 tacaatgtat ggctatatat gtattcatcc gaaggcaaat aaaatcaatc aactttattt
     3661 atgcaaacat agtttattgg cagttgatat gtttacagaa aatcaggatt tgacttcctg
     3721 ttttttaatt tgctcggtaa ccaattttga ataaaagcca ttttgttgca gcaatgacag
     3781 atgtgtgcca cgctcaagta ttttgccctg atgcaataca attatttcat cggcatgttt
     3841 aacagatgaa atgcgatgac ctaaaattaa tgttatgcga tgttggaaat agggtaacag
     3901 gttttgcaac aattgttctt ctgtgccggt gtcgagtgca gaaagacaat catcaagaat
     3961 caaga
//
LOCUS       DAORQT010000143         3963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4155_length_3963_cov_5.556807, whole genome shotgun sequence.
ACCESSION   DAORQT010000143 DAORQT010000000
VERSION     DAORQT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4155_length_3963_cov_5.556807"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PK134_05800"
     CDS             <1..1117
                     /locus_tag="PK134_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38397.1"
                     /translation="FSQGISDYTDIAFDNNGTPYVAYSDHANNDNVTVMKFDGSSWVA
                     VGNVGFSTGEPYYNSLAINSNGVPYVVFQDATSLSKATVMKFDGNSWVVVGTAGFSSV
                     SVKEPTIAIDNNNTPYVAFTQWGGLYNASVMKFDGSNWVNVGPVGFTTNSADYASLAF
                     SNSGEPYMAFIDGTQNSHVSVMKFNGSSWALVGNAGFSTGTSHYPSLAFSNTGEPYVA
                     FKSSFNNSQATVMKYDGSAWVAVGSTGFSSGWADYTSLAFNSSGVPYVAFEDINNSYR
                     ATVMKFGNGTSSINSMAADNSIEVYPNPANDYLNLTNLPVGANIKINDAIGKNVYDAS
                     ITTKETSINTSAFTNGIYHICIEKDGVLINKKLVINK"
     gene            complement(1254..1844)
                     /locus_tag="PK134_05805"
     CDS             complement(1254..1844)
                     /locus_tag="PK134_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB38398.1"
                     /translation="MKKYYVLYKPYGYLSQFSDEAKHKGLKHLIDVEPDVYPAGRLDV
                     DSEGLLLLTNDNFLKHHLLNPENKHKRTYHVQVDGTATSVHLNLLRQGVEITIEGKPY
                     KTFPPASVNLLNEVSYPERNPPVRFRKHIPTSWIELVLTEGKNRQVRKMTAAVGIPTL
                     RLIRSKIEAINIKDYQPGELKELSQKEIFKLLHLQI"
     gene            1990..2571
                     /locus_tag="PK134_05810"
     CDS             1990..2571
                     /locus_tag="PK134_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRB38399.1"
                     /translation="MVDVQELKNILCRYYNSVSDKTIDKIAQHFEYRNYKKGDTVVSN
                     KSICTQCFFICSGYTRGYIYREDAQFTLWFGEPGDMITSFSTMFQNEKGNEIIETLTD
                     CTMLAINMQTFKKLISEEIEIANVYHKIVEAGYLFWERRFLIYSQERAEDRYKEWYNR
                     SKHISEHISLGVIAQYLNIDQATLSRIRSKIKK"
     gene            complement(2612..3745)
                     /locus_tag="PK134_05815"
     CDS             complement(2612..3745)
                     /locus_tag="PK134_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38400.1"
                     /translation="MKNRTILFAFFLLLVSIGYSQTCTVGYPPPSDNCATAPAIQLNG
                     VYCGNTCNATANDAAWFKNSNEFCNSVSKQTIENNGFYIFQATASSVNFTICTKTGCT
                     RKNNIIGASGIQALIFNFPTANGLGTCGSGSIDGYYCLKQLSGTDCGSCSNPKGCLSA
                     SVNGLSAGKFYYFMVDGYEGDCCDFSIQFTSNITLPVELISFKGKGENAGNHLFWSTA
                     SEKNNDYFNIESSTDATNWRTINSIKGHGNTNQLKNYDFIDLNPAAVITYYRLMQVDY
                     DGSFSYSPIIFVDRSATGSLSIFPNPFNSELNLQFNISKGGLYTLTFNSMLGKQIQKQ
                     IDLKEGQSLAKIDFSDNLTSGAYMVEVANESGEILQHVKLIKY"
BASE COUNT         1285 a          636 c          728 g         1314 t
ORIGIN      
        1 attttctcaa ggcatttcag actatacaga tattgctttt gacaataacg gtacaccgta
       61 tgtagcatac agtgatcatg caaataatga taatgtaacg gttatgaaat ttgatggcag
      121 cagttgggta gctgtgggta atgttggatt ttcaactggt gaaccttatt acaattcatt
      181 agccatcaac agcaatggag ttccttatgt cgttttccaa gatgcaacaa gtttatctaa
      241 agcaacagta atgaagtttg atggcaatag ttgggttgtt gttggtactg ccggattttc
      301 cagcgtatcg gttaaagagc caacaattgc aatagataat aataacactc cttatgtggc
      361 ctttacacaa tggggtggct tatataatgc atctgttatg aaatttgatg gtagtaattg
      421 ggtaaacgtt ggtccggttg gttttacaac aaattctgca gactatgctt cattggcatt
      481 tagcaatagc ggagagcctt atatggcttt tattgatggc acgcaaaata gccatgtttc
      541 tgtaatgaag tttaatggca gcagttgggc attggtagga aatgcaggtt tttctacagg
      601 aacttcacac tatccatctt tagcattcag caatacggga gaaccctatg tggcatttaa
      661 gagtagcttt aacaattctc aggctacagt gatgaagtat gatggcagtg cttgggtggc
      721 tgtggggtca acaggctttt catcaggatg ggcagattat acatctcttg cattcaacag
      781 tagcggtgtg ccttatgttg cttttgagga tataaataac tcgtatagag caacagtgat
      841 gaaatttggt aatggaacat caagcataaa cagcatggct gcagataata gtatagaagt
      901 ttatcctaat ccggctaatg actatctgaa tcttactaat cttccggttg gtgctaatat
      961 caaaatcaat gatgcaatag gcaagaatgt ttatgatgct tcaattacaa ctaaggaaac
     1021 aagcatcaac acctctgcat ttacaaatgg tatttatcat atctgtattg aaaaagatgg
     1081 tgttcttatc aataaaaaat tggtgataaa taagtaattt attttataaa aagtaaaccc
     1141 catttcctga tggaaatggg gtttttcatt ttctgagaaa gccattgttc actaaaggat
     1201 attaattacg tctgagaaat ataccggtag cttgaatgct actttgcatt tgttcagatt
     1261 tgtaaatgca ataatttaaa aatttctttt tgcgacaatt cttttaattc acccggctga
     1321 taatctttaa tattaatagc ttcaatttta cttctgatta gtcttagagt aggaatacca
     1381 acagcagcag tcatttttct aacctgacgg tttttacctt ctgtaagaac caattctatc
     1441 cagcttgttg gaatgtgttt gcgaaaacgc acaggcggat ttctttcagg ataagaaacc
     1501 tcattcaata aatttacaga tgcaggagga aacgtcttat atggcttgcc ttctatagta
     1561 atttcaacac cctggcgaag taggtttagg tgtacacttg tggctgtacc atcaacctgc
     1621 acatgatagg ttcgtttatg tttgttttcg ggatttaata aatgatgttt caggaaatta
     1681 tcattggtaa gcaataataa accttcgcta tcaacatcaa gtctgcctgc aggataaaca
     1741 tccggttcaa catcaatgag gtgtttaagt cctttgtgtt tggcttcatc actaaactgc
     1801 gatagataac cataaggttt ataaagtaca taatattttt tcacgagtta aaaatactgg
     1861 ttttgattca ttgaaaatga aatactttaa gcattattaa atcctgacac ataatgttga
     1921 ttaattttta tctttgttaa acttgatttt gaaatgtaaa ggagtcaata ccgcttaaat
     1981 gcctttaaaa tggtagatgt tcaggaacta aaaaatatac tttgcagata ctataattct
     2041 gttagtgata aaaccattga taaaatcgca cagcattttg aatacagaaa ctataaaaag
     2101 ggcgatacag tagtttcaaa taaaagtatt tgcacacaat gcttttttat ttgctccggc
     2161 tatacaagag gatatattta tagagaagat gctcagttta cgctctggtt tggggagcct
     2221 ggcgatatga ttacttcatt ttcaaccatg tttcagaacg aaaaagggaa tgaaattatt
     2281 gaaacactga ccgactgtac tatgcttgct ataaacatgc aaacatttaa aaagttgatt
     2341 tccgaagaaa tagaaatagc taatgtttat cataaaattg ttgaagcagg atatcttttt
     2401 tgggaacgaa gatttttgat ttattcacaa gaacgtgcag aagacagata taaggaatgg
     2461 tataatcgct caaaacatat ttcagaacat atttcgcttg gtgtgattgc acagtatttg
     2521 aatattgatc aggcaacatt aagccgcatc cgttctaaaa taaaaaagta attttttttt
     2581 tggttaactg caaaagggaa aaagggtagt tttagtattt gattagcttt acatgttgta
     2641 aaatttctcc tgattcattt gcaacttcaa ccatataagc accacttgtc agattatcag
     2701 aaaaatcaat ttttgcaaga ctctgacctt cttttaaatc tatttgtttt tgaatttgtt
     2761 tacctaacat tgaattgaaa gtaagcgtgt aaagcccacc tttacttatg ttgaattgta
     2821 aattcaactc tgaattgaaa gggttgggaa atatacttag cgaacctgtt gcagaacgat
     2881 ctacaaaaat gattgggctg tatgaaaatg aaccatcata atcaacctgc ataagtcggt
     2941 agtaggtaat aacagcagca gggttcaaat caatgaaatc ataatttttt aattgattcg
     3001 tattgccatg ccctttaata ctgtttatag tgcgccaatt tgtagcatcg gtgctgcttt
     3061 ctatattaaa ataatcatta tttttttctg atgctgtact ccagaacaaa tgattgcctg
     3121 cattttctcc cttgcccttg aaactgatta actcaacagg taatgtaatg tttgacgtaa
     3181 actgaatact aaaatcacaa caatcacctt cataaccatc aaccataaaa taataaaatt
     3241 tacctgcaga caatccattg acacttgcac tgagacaacc ttttggatta ctacatgaac
     3301 cacagtctgt gccgctaagt tgctttaaac aataatagcc atcaatagaa cctgaaccgc
     3361 atgtccccaa tccatttgct gtagggaaat taaaaatcaa tgcctgaatt cctgatgcac
     3421 caattatgtt gttctttctt gtacatcccg tcttagtaca tattgtgaaa tttactgatg
     3481 atgcagttgc ctgaaaaatg tagaaaccat tattttctat ggtttgtttg ctaactgaat
     3541 tacaaaattc attactgttt ttaaaccatg ctgcatcatt tgctgtggca ttgcatgtat
     3601 ttccgcaata aacaccatta agctgaattg caggtgctgt tgcgcaattg tcagatggag
     3661 gagggtagcc tacagtacag gtttgactgt aacctattga taccagtaat aggaaaaatg
     3721 caaatagaat ggttcggttt ttcataatct acattttatg gtaaatctga ctgtataaaa
     3781 gtagaatttt aaaaagtgat ttgatttgat gtaggtcaaa aaatgaaaat ttgtaattcc
     3841 cctaaaaact taagcttttt tatcaattta aaaagggtgg gtgtaagata aaaagcactt
     3901 gatgccatag aacattagtg ctgttttatc ataggaaaat tgttgtttaa tatagtacaa
     3961 cta
//
LOCUS       DAORQT010000144         3962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4157_length_3962_cov_9.808037, whole genome shotgun sequence.
ACCESSION   DAORQT010000144 DAORQT010000000
VERSION     DAORQT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_3962_cov_9.808037"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PK134_05820"
     CDS             complement(<1..281)
                     /locus_tag="PK134_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HRB38401.1"
                     /translation="MKNDFKNEKWSLVKPPFRFTNNLKIEVSNFGRVRTSTKIAENRV
                     LKGTLINGYPIVRFKLFKKRADDAQKQLDQKRRKIEQLTGKLRDVKSEL"
     gene            complement(342..707)
                     /locus_tag="PK134_05825"
     CDS             complement(342..707)
                     /locus_tag="PK134_05825"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRB38402.1"
                     /translation="MLQVTGILKQKFAEQVVTDKFRKREFTLTIDAKSAYPQIVIFQL
                     ANDKCRLIESFQPGDELSVSFNIKGREWKSPSGEIKYFTSLDAWKIERTSAGESPMES
                     PMPSMSDLPQEDYKDDLPF"
     gene            complement(739..1863)
                     /locus_tag="PK134_05830"
     CDS             complement(739..1863)
                     /locus_tag="PK134_05830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38403.1"
                     /translation="MKNSGKHLHIISFDIPYPANYGGVIDVFYKLRALVSAGIKIHLH
                     AFQYGRQSSKILKELCESVHYYQRDVSKSQLFLKEPYIVVSRSSEQLIKNLLSDEHPI
                     LFEGLHSTHYLSDERLKKRKKIVRTHNIEHEYYKNLAKAESNIFKRYYFYNESSKLEI
                     YENILSNADVIATISPNDTQYFKSKYGNAHYIPAFHSFTKVNSRIGKGAYAFYHGNLA
                     IGENDMAALYLVQEVFKNSPVTLLIAGSKPGVNLRDAVQRKNNITLLSDLNTKQINDL
                     VLDAHINVLPTFQPTGIKLKLLSALFSGRFALVNTPMVQNTGLEQLCTVADTPEAMRE
                     ALSAMIKQEFTQIDISLREEILMENFNNKANALKLKNLIF"
     gene            complement(1887..>2534)
                     /locus_tag="PK134_05835"
     CDS             complement(1887..>2534)
                     /locus_tag="PK134_05835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38404.1"
                     /translation="YGNDVKVVRNVPDRTDSVNFDVAKWKRSVGFPDYKKVFIMQGAG
                     INVDRGAEEAIEAMKDVDAMLLIIGGGDVFHQLQELAIKHNVKHKVIIHPKISREELI
                     TVTASCFAGLTLDKDTNINYKFSLPNKLFDYIHAGIPVIASDLPEVTAIVNGYKTGIV
                     LPQYSPQAIAEAMNRLVIEKELYVSYKENCSIASKDLCWQKEKKILIDVILSSEK"
     assembly_gap    2535..2634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2839..3702)
                     /locus_tag="PK134_05840"
     CDS             complement(2839..3702)
                     /locus_tag="PK134_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRB38405.1"
                     /translation="MRQLRIQKSITNRESQSLEKYLQEISREGMITAEEEANLARRIR
                     EGDEEALSKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAASRFDETRGFKF
                     ISYAVWWIRQSILQAIAEQARIVRLPLNQVGSLRKINKVFSKLEQEFEREPSAEEIAE
                     MMEVPIEKISDTLKSPGKHVSMDAPFAGNEEATLLDVMQNDDAPDADFGLMNESLRKE
                     IDRSLKTLTERERDVVKLFYGIGVSHSFSLDEIGAKFGLTRERVRQIKEKAIRRLRSG
                     SRNKLLKDYLA"
     regulatory      3861..3961
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1156 a          751 c          617 g         1338 t
ORIGIN      
        1 tttaattctg attttacatc gcgcagttta cctgttagtt gttcaatttt tcttcgtttt
       61 tggtccaatt gcttttgtgc atcatcagct cttttcttaa aaagcttaaa cctcacaatc
      121 ggatatccat ttatcaaagt tcctttcagc actctgtttt ctgcaatttt ggtggaagtt
      181 ctgaccctcc caaaatttga tacttctatt ttcagattat tggtaaacct aaacggaggc
      241 tttacaagcg accacttttc attcttaaaa tcatttttca ttgtttgaaa atttacaatt
      301 agaattcaat tgtgatttaa tgattcaata aatttaactg attaaaatgg caaatcgtct
      361 ttataatctt cctgtggtaa atctgacata gatggcattg gcgattccat tggggactct
      421 cctgcagaag ttctttcaat tttccatgca tcgagcgatg taaaatactt aatctcaccg
      481 gatggtgatt tccactctct gccttttata ttgaaggaaa ccgaaagctc gtcacccggc
      541 tgaaaactct ctattaacct gcacttatca tttgccagtt gaaaaataac tatttgagga
      601 taggctgact tagcatctat tgtaagtgta aattctcttt tgcgaaattt gtctgttact
      661 acctgttctg caaatttctg ttttaatatt cctgttacct gaagcatttt gaattttttt
      721 tcaaaagtaa aaataaattt aaaaaatcaa attttttagt tttaatgcat tggccttatt
      781 attgaagttt tccataagaa tttcttcccg tagcgaaata tctatctgag taaattcctg
      841 tttaatcatt gctgaaagtg cctcacgcat agcttcaggt gtatcggcta cagtgcacaa
      901 ctgttccaat cctgtatttt gaaccattgg tgtatttacg agggcaaaac gaccgctaaa
      961 aagtgcagaa agtagtttaa gcttaattcc tgttggttga aaagtgggta gcacatttat
     1021 atgagcatcc aaaactaaat cgttaatctg cttagtgttt aaatcactta gcaaagtaat
     1081 attattcttt ctttgtacag catctcttaa attaacaccg ggtttgctac cggcaatcag
     1141 caaggtaacc ggtgagtttt taaaaacttc ttgcactaaa tagagagcag ccatatcatt
     1201 ctcaccaata gccagattgc cgtgataaaa ggcataagct cctttaccaa ttctcgaatt
     1261 tacttttgta aaggaatgaa aagcaggtat gtaatgtgca ttgccatact tacttttaaa
     1321 atattgtgtg tcgttaggcg aaatggtggc aatcacatct gcatttgata aaatgttttc
     1381 gtagatttct aacttactcg attcattata aaaatagtat ctcttaaaga tgtttgattc
     1441 tgcctttgct aagtttttat aatattcatg ttctatatta tgcgtcctga ctattttctt
     1501 tcgcttcttt agtcgctcat cagacaaata atgagtacta tgtaatcctt caaatagtat
     1561 tggatgctca tcggaaagca ggtttttaat cagttgctct gacgagcgtg acacaacaat
     1621 atatggttct tttaaaaaca actgcgattt ggaaacatca cgttgatagt agtggacaga
     1681 ttcacataac tctttaagaa tttttgatga ctgacgacca tattgaaatg catgaaggtg
     1741 aatttttatt ccggcagaaa caagagcacg gagcttataa aacacatcaa tgactccacc
     1801 atagtttgca ggatatggaa tatcaaaact gataatatgt aaatgcttac cgctgttctt
     1861 cacaatgtgt tttaaagttt gtttggctat ttctcagagg acaaaattac atcaattaat
     1921 atttttttct ctttttgcca gcataaatcc ttagatgcaa tactacaatt ttctttatag
     1981 ctaacataaa gttctttttc gataactaaa cgattcattg cctctgctat tgcttgtgga
     2041 gagtattgtg gcaacacaat ccccgtttta tatccgttca caattgctgt aacctcaggt
     2101 aaatctgaag ctataacagg aatgcctgca tgaatgtaat cgaatagttt gtttggcaaa
     2161 ctgaatttat aattgatgtt ggtgtcttta tccaatgtaa gtcctgcaaa gcatgatgct
     2221 gttacagtaa ttaattcctc gcgtgaaatt ttagggtgaa taatcacttt gtgttttaca
     2281 ttatgtttga tagccaattc ttgtaactga tgaaaaacat ctcccccacc tataataagt
     2341 aacatggcat ctacatcttt cattgcttca attgcctcct ctgcgcctct gtcaacatta
     2401 attcctgcac cttgcattat aaagaccttc ttgtaatcag gaaatccgac agaacgcttc
     2461 catttcgcta catcgaaatt aacgctatct gttctatcag gaacattacg aacaactttc
     2521 acatcattac catannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaccg
     2641 gtcatatcat aaacaggtaa actaagtggt aactttcgac caacaataag tatttgatag
     2701 ttaagctcag aaaatactga tgctgttctt ctaacccgct gatcggttgc tatatcatta
     2761 attacagaaa ttataacctt cttttgcgcc attcttagtt gatgtggcga aggtaaaact
     2821 ttgcttgtta aatttaaatc aggctaaata atctttaagc aacttgtttc gagagcctga
     2881 gcgcaatctt cttatagctt tctcctttat ttgcctgacc cgttctcttg ttaaaccaaa
     2941 cttagcccct atttcatcca atgaaaaact atgcgaaaca ccaatgccat aaaacaattt
     3001 aaccacatca cgctcacgct ctgtcaatgt ttttaaagag cgatctatct ctttacgaag
     3061 tgattcattc atgaggccaa aatctgcatc gggtgcatca tcattttgca taacatcaag
     3121 taaggttgcc tcttcattac cggcaaatgg cgcatccatg ctgacatgtt taccaggtga
     3181 tttcaatgta tctgaaattt tttcaatcgg cacttccatc atctctgcta tttcctcagc
     3241 tgagggttca cgctcaaact cttgttccaa ttttgaaaaa actttattta tttttcttaa
     3301 cgaaccaacc tgatttaaag gaagcctcac aatgcgtgcc tgctcagcaa tggcttgcaa
     3361 aatactttga cgtatccacc aaacagcata agaaataaat ttaaatcctc tggtttcatc
     3421 aaaacgtgat gctgctttaa tgagtccgag attgccttca ttaatcagat caggaagact
     3481 tagtccctga ttctgatatt gcttggcaac tgacacaaca aatcttaaat tagctcttgt
     3541 taatttactc aatgcttcct catcaccttc tcgaattctt cgagctaaat tggcttcttc
     3601 ctctgcagta atcatgcctt cgcggctaat ttcctgaagg tacttttcca acgattggct
     3661 ttctctgttg gttatggact tttgaatgcg cagctgtctc ataaattgtt tcggtttatc
     3721 ttacaaagtt aatagtaaaa aaatcattaa tccaaaaaaa cattcaacca aaatgcatca
     3781 tttgtttaaa aagatttcaa caaactgaaa aaagtcatta caggattttt tgtgatttcg
     3841 gaaaatgaat gattactttg tatcatcaaa aggggtgctt cctataatga atggaatgct
     3901 gagatcatac ccataacctg atcaggataa tgcctgcgta gggataggta acattctttt
     3961 at
//
LOCUS       DAORQT010000145         3955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4169_length_3955_cov_8.353846, whole genome shotgun sequence.
ACCESSION   DAORQT010000145 DAORQT010000000
VERSION     DAORQT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4169_length_3955_cov_8.353846"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PK134_05845"
     CDS             complement(<1..803)
                     /locus_tag="PK134_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38406.1"
                     /translation="MGVIQRQGLKNTITTYLGIAIGFVSLIVIQPQFLTKEELGLTRI
                     LYSFSILVAMFVPLGIGNATTKYFPLFKDSNKNHHGYFAFMMLFPLAGFVLAAAILFA
                     IKNFIIAQYISESPMFTEFYYYVFPLTFILSLITCLNVYCYANYKSTIPAFLNDVVAR
                     VLVIVVITVYFLKWITLNQFIACYVGLYGIQLLMLLAYIFIFDKPVFKIDWNYFKEKD
                     FKTLIRYGLLLWFAGVASIGLKYLDSIMLGKYLSLSFVGIYTIAAFIPTV"
     gene            885..2297
                     /locus_tag="PK134_05850"
     CDS             885..2297
                     /locus_tag="PK134_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38407.1"
                     /translation="MKKLLLYINVIALMIIAIDVWGQNESNIWYFGNHAGIDFNSGSP
                     VAITNGGMMTYDNASAICDSAGNLLFYTDGQMVWNANHQIMSNGVNIGGSTTGGQAAV
                     IVKKPLSNSIYYIFTSDGFGASGGLEYSIVDMSLLGGLGEVTQLGTLLQIPSTEKLAG
                     IHTCTEKFTWLITHRGNSNEFCSYKITQSGLDTVPVVSAIGTFIPAGPYGVVNSTAGQ
                     MSLSKDGSKLASVNYQSGTVEIFDFNVASGHITNQILLTNTYLHAWGVEFSADASKLY
                     LSVYNSPSILQFDLNSGNATTITGSEISIGNISGTYCPGFSGATGYLQRGPDDKIYLA
                     KCGSTYISVINSPNDAGTLCGFVDNGIYLNSKVSTFGLSRSIAHECESTGVKNVDKNE
                     LFSVTPNPSNGSFSINFSENNFRGIRVLKIFNSMGEVVYFEKVDNSETKNVSTNLAAG
                     IYTLELNLDNQSVFKKIVVY"
     gene            complement(2294..>3955)
                     /gene="asnB"
                     /locus_tag="PK134_05855"
     CDS             complement(2294..>3955)
                     /gene="asnB"
                     /locus_tag="PK134_05855"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB38408.1"
                     /translation="GEIYNHAELRKQLEQEGLSFRSQSDTEVILQSFSRWGISCIQKF
                     IGMFAIALYDSKEEKVYLIRDRAGVKPIYWYFKNDLFLFGSELKAFHRHPSFQKEIDH
                     GAISLYFQHGYIPAPHCIFKNTYKLLPGHYLTIDLRSKEIRNSCYWDVSDFYNKPKLA
                     ISETEAITETEKILTKAFNYRMVADVPVGIFLSGGYDSSLVTALLQKERTQKLKTFTI
                     GFEQESYNEAKHARKVAEHLGTEHYEYTCTYKEAMDIIPMLPEISDEPLADSSLIPTY
                     LVSKMARKQVTVALSADGGDETFAGYTKYAKATDYLNKLQKLPSIAKTAAQIPALLWK
                     MTSSNSLSIPDRADKLQLILRAKHPVEAFNIITQGMTQNEVSSLLMNHITFPLSAFDD
                     FDKLKAPELLDNFLWLDYKTFLSDDILQKVDRATMAVSLEGREPLLDHNIIEWAAQLP
                     SAFKLNNGTSKYLLRKITHQYIPKEIMEREKMGFQIPLHLWLKSEMKDLLLHHLSEES
                     ISKYQIFKQAEVNAILNAYLNNNNLDFYRLWKLFNLQMWLDRWTT"
BASE COUNT         1298 a          635 c          745 g         1277 t
ORIGIN      
        1 actgtgggga taaatgctgc aatggtgtaa atacccacaa aactcagcga taaatatttt
       61 ccaagcataa tgctgtctaa atattttaat cctattgaag ctactcctgc aaaccacagc
      121 agcaacccgt agcgtatgag tgttttaaaa tctttttctt taaaataatt ccagtcaatt
      181 ttaaaaacgg gcttatcaaa aataaaaata taggctaata acatcaacag ttgaatacca
      241 taaagcccca cataacaggc aataaactga tttagcgtaa tccatttcag aaaataaaca
      301 gtgataacaa caatcaccag cactcttgca actacatcat ttaaaaaagc aggaatagtt
      361 gacttgtaat tggcataaca gtaaacattc aggcaggtga tgagtgaaag aataaatgta
      421 agcggaaaaa cataataata aaactcagta aacattggcg actcgctaat gtattgtgct
      481 atgatgaaat tttttattgc aaaaagaatt gctgctgcca aaacaaatcc ggctaatgga
      541 aacagcatca taaaggcaaa gtagccatga tgatttttat tgctgtcttt aaacagcgga
      601 aaatatttgg tagtggcatt gccaatgcca agaggcacaa acatggcaac taaaatggag
      661 aaggagtaca gaatacgggt aagaccaagc tcttcctttg taagaaactg cggctgaatt
      721 acaataagac tcacaaagcc aatggcaata cccagataag tggtaatggt attttttaat
      781 ccttgccgtt gaattacgcc catgaaaaat aattattcct gcttgtttat ttaaaaaata
      841 agtgtaattt tgttagcaag agtaactaat aaataaatta agtcatgaag aaattattac
      901 tttatatcaa cgtcatagcg ttgatgatta tagcaataga tgtttggggg caaaacgaat
      961 ccaacatttg gtactttgga aaccatgcag gaatagattt taactcaggt agtcctgttg
     1021 caattacaaa tggaggaatg atgacgtacg ataacgcaag tgctatttgt gattcagcag
     1081 ggaatttatt gttttataca gatggtcaaa tggtttggaa tgcgaatcat cagataatga
     1141 gtaatggcgt taatataggc ggtagtacaa caggaggaca ggcagcagtt atagttaaga
     1201 agccattaag caattcaatt tattatattt ttacttcaga tggttttggt gcttcaggtg
     1261 gtttggagta ttcaattgta gatatgagtt tattaggggg attaggtgaa gttactcaat
     1321 taggtacatt acttcagatt ccctcaactg aaaaacttgc cggtattcat acttgtactg
     1381 aaaagtttac ttggttaata acccatagag gaaattcaaa cgaattttgt agttataaaa
     1441 ttactcaaag cggattagat acagtacccg tggttagtgc tattggaaca tttattccgg
     1501 cagggcccta tggtgttgtg aatagtacag cagggcagat gagcctttct aaagatggaa
     1561 gtaaattagc aagtgtaaat tatcaaagtg gaactgtaga gatttttgat ttcaatgtgg
     1621 catctggcca tataaccaat caaatcctac taactaatac atatcttcat gcttggggag
     1681 ttgaattttc ggctgatgca tccaaattat atttatcagt ttataactct ccttcaattt
     1741 tacagtttga tttaaattca ggaaatgcaa ctacaataac tggttctgaa attagcattg
     1801 gaaatatatc cggaacttat tgtcccggat tttctggagc cacagggtat cttcaacgtg
     1861 gacctgatga taaaatttat ttagctaagt gtggttcaac ttatattagt gtaatcaact
     1921 ccccaaatga tgcgggaaca ctttgtggtt ttgtagataa tgggatttat cttaattcta
     1981 aagtctcgac ctttggctta tcaagatcaa ttgcacatga atgtgaaagt actggcgtaa
     2041 agaatgttga taaaaacgaa ttgttttcag tcacacccaa cccaagtaat ggaagtttta
     2101 gtattaattt ttcagaaaac aattttagag ggattcgagt attgaaaatt tttaattcaa
     2161 tgggagaagt agtttatttt gaaaaagtag ataattctga aacaaagaat gttagtacaa
     2221 atcttgcagc cgggatttat actttagaat taaatttaga taatcaatca gtttttaaaa
     2281 agattgttgt ttattaagtt gtccacctat caagccacat ttgaagatta aataatttcc
     2341 acaagcgata aaaatcaaga ttgttgttat ttagatatgc attgagtatt gcattaactt
     2401 ctgcctgttt aaatatctga tatttagaaa tagattcttc agaaagatga tgtaataata
     2461 aatctttcat ctcgctcttt agccaaagat gtagtggtat ttggaacccc atcttctccc
     2521 tttccattat ttcttttgga atatattgat gtgttatttt gcgcaacaga tattttgagg
     2581 ttccattgtt gagtttaaac gcagagggta attgagcagc ccattcaata atgttgtggt
     2641 cgagcagagg ttctctgcct tcaagactta cagccatcgt tgcgcggtca actttctgta
     2701 aaatatcgtc agaaagaaaa gttttataat caagccataa aaaattgtca agtagttcag
     2761 gtgcctttaa cttatcaaaa tcatcaaatg cagaaagtgg aaaagtaata tggttcatta
     2821 aaaggctgct aacttcattt tgtgtcatac cctgagtgat aatattaaat gcttcaacag
     2881 gatgttttgc tcttagtatc agttgcagtt tgtctgctct gtccgggatg gaaaggctgt
     2941 ttgatgaagt cattttccag agtaaagccg gaatctgtgc agctgttttt gcaatgctcg
     3001 gtagcttttg aagtttatta agataatcgg ttgcctttgc atatttggtg tagcctgcaa
     3061 aagtttcgtc accgccatcg gcactcaatg caacagtaac ttgtttgcgt gccatcttac
     3121 tgactaaata cgtaggtatc aatgaagaat cagctaatgg ttcatcggat atttcaggga
     3181 gcatagggat aatatccatt gcttctttat aggtgcaagt gtactcatag tgctccgttc
     3241 ctaaatgttc agctaccttg cgtgcatgtt tggcttcatt ataactttct tgttcaaaac
     3301 cgatagtaaa tgtctttagc ttttgagttc tttctttctg caataatgct gtcaccaatg
     3361 agctgtcata tccaccactt aaaaatatgc ctacaggaac atcagcaacc atacggtagt
     3421 taaaagcctt ggttaatatt ttttctgttt cagttattgc ctcagtttca gaaatagcaa
     3481 gtttaggttt gttataaaaa tcggatacat cccaataaca tgagttacgg atttctttac
     3541 ttcttaaatc aattgttaag taatgaccgg gtaaaagctt gtatgtgttt ttaaaaatac
     3601 aatgtggagc aggaatgtat ccatgttgaa aataaagact aatggcaccg tgatctattt
     3661 ctttttgaaa tgaaggatgg cggtgaaatg cttttaattc agaaccaaaa agaaataagt
     3721 cattcttaaa ataccaatat ataggtttta caccggcacg atctcttatc agataaactt
     3781 tttcttcttt tgaatcatat aatgcaatgg cgaacattcc aataaatttc tgaatgcaac
     3841 taattcccca tcgtgagaaa gactgaagta taacttctgt atcgctttgc gaacggaatg
     3901 aaagcccttc ttgctctaat tgtttgcgta gttctgcatg gttataaatt tcacc
//
LOCUS       DAORQT010000146         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4216_length_3925_cov_6.928424, whole genome shotgun sequence.
ACCESSION   DAORQT010000146 DAORQT010000000
VERSION     DAORQT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4216_length_3925_cov_6.928424"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..447)
                     /gene="rpsT"
                     /locus_tag="PK134_05860"
     CDS             complement(193..447)
                     /gene="rpsT"
                     /locus_tag="PK134_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRB38409.1"
                     /translation="MANHKSAVKRIRANGAKKDRNRYQAKTSRNALKELRTTTDKKEA
                     EKLLPEVSGMLDKMAKKNLIHKNKAANLKSSITKYVSALK"
     gene            complement(498..569)
                     /locus_tag="PK134_05865"
     tRNA            complement(498..569)
                     /locus_tag="PK134_05865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(534..536),aa:Glu,seq:ttc)
     gene            complement(793..3189)
                     /locus_tag="PK134_05870"
     CDS             complement(793..3189)
                     /locus_tag="PK134_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38410.1"
                     /translation="MRYLNNAKKSFLLIIANVLFGISCYAQMGVLRGNVYLKQNGEPI
                     AGAGIFPVNDKSIGVASDADGSYEINLPVGKQTIVYTLTGLKNDTLTLDIKDGTESIR
                     NIYLTSNLKNLNVVVVSASKYEQRIEDITVSMEVISPKLIENRNTTNVTSVLEQTPGV
                     TILDQEPQIRGGSGFSFGVGSRVATLIDGLPIMTGDAGRTEWQFIPVENIEQIEVIKG
                     ASSVLYGSSALSGTINFRTAYPKERYHTYLRTYGGVYDKPSNRDAKWWDGPATFSGIN
                     FLHSEKINRWDLIIGGNGLYDHGFIGPPLAMSSLPFQDTTISNNDVASRWGRLNFNIR
                     HRFKNVEGLSAGINGNFMQSHSNFSLVWANDSTGIYNAFPKTMTLTDSKTFYIDPYIS
                     LTGKKGSSHQLRSRIFYADNDNGNDQSNKTNVYLAEYRFNRELISLGRLNVTSGVYFQ
                     QSFSHASLYAGSGSDKNTLENYAAFIQLEKKLIKKLMVSAGFRTEYFEMNNKESQLEP
                     ILRAGLNYQLTEGTFIRCSYGQGYRYPTIAEKYIETGAGGISVFPNPEVQPESSWNAE
                     AGIKQGFKIGNFFGFADFAVFEQRYHNTIEYIYALWQPDQLAGFKFVNTGNSRVKGLE
                     VSIMGEGKPVHNFAISFLGGYTYTLPQSLEPDYIFATDNPPTDFLPDSLSYSSTSTDT
                     SKNILKYRFQHIAKMDIELTWKFISLGYGIRYYSFMKNIDKKFYDLDETVLPTGVKAY
                     REKHDKGTLVHDIRLGFKLNEIFKASFLVNNATNLEYSLRPLKIESPRNYTVQLTAAF
                     "
     gene            complement(3191..3673)
                     /locus_tag="PK134_05875"
     CDS             complement(3191..3673)
                     /locus_tag="PK134_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38411.1"
                     /translation="MQLLGTTNTDTIGYVRFETPNATVSTYTRFAAAVNYLSTATPSN
                     TIWILSSSKGVGPIVNSQIFIDDIAMVFNPSSVNTIDTKNEVSILGNVIKEKIRFNYK
                     NTGVYQFKLIDTAGRIVTDTELNSQQSTVDVSALDGLYFYIIENNSGIINNGKVIIRN
                     "
BASE COUNT         1166 a          731 c          708 g         1320 t
ORIGIN      
        1 gaacggatga ggatagctag caactcactg tcggttaagg cttccttgcc tttttcaagg
       61 agtttctctc ttggcctgtc actttccgac catgatttga tgtttttagg ttcttccatg
      121 gtgctaagat aaggtgttaa acaaaaaaag cccgcattta tagcgagctt aaaattcaaa
      181 attatgtgaa tattatttta aagcactaac atatttagtg atacttgatt tcagatttgc
      241 agccttattt ttatgaataa ggttcttttt tgccatttta tcaagcatac ctgatacttc
      301 cggaagcaac ttttcagctt ctttcttatc tgtggtagta cgcagttctt tcaaagcatt
      361 acgcgaagtt tttgcctgat agcggtttct gtctttttta gcaccgttgg cgcgaattct
      421 ttttactgct gacttgtgat ttgccataat tacagttttt actttttaat gatggatatt
      481 aatccttatt ttttttgtag cccgtacgag aatcgaactc gtgtttcctc cgtgaaaggg
      541 agatgtccta acccctagac gaacgggcca aacgtttttt tgaggctgca aatgtataaa
      601 aacaatttac attcaaagcc ttatagtgta aatatttttc atttttctgc tatttggctg
      661 aagtattctg acttctgtat agaaaatcat cattaacaac ctaagtaaga ctgaattaaa
      721 gctattgaac aggtcagatt taacagccca aaataaactc ataaacacct ttttaacaag
      781 ctaaggtcta tattaaaatg ctgccgtaag ctgaacagta taatttctgg gcgactcaat
      841 ttttaacgga cgcaatgaat actcaaggtt tgtggcattg tttactaaaa atgaagcttt
      901 aaaaatttcg ttgagtttga agcctaaacg aatatcgtgc accaaggttc ctttgtcatg
      961 tttttcgcga taggctttta caccggtcgg taaaactgtt tcatcaagat cataaaactt
     1021 tttatcaata tttttcatga aactgtaata acgaatgcca taacctaaag aaataaattt
     1081 ccaggttagc tctatatcca ttttggcaat atgctgaaaa cggtatttga gaatattctt
     1141 tgatgtgtct gtgctggtgc tgctgtatga taatgagtcg ggtaaaaagt ctgttggcgg
     1201 attatcagtt gcaaaaatat agtcaggctc cagactttgc ggcagcgtat aagtatatcc
     1261 acccagaaag cttatggcga aattgtgaac cggttttcct tcacccataa tagaaacttc
     1321 aaggccttta acacgtgagt ttcctgtatt cacaaattta aatccggcta actgatcggg
     1381 ttgccataaa gcataaatat attcaatggt gttgtgatag cgttgttcaa aaactgcaaa
     1441 atcggcaaag ccaaagaagt ttccaatctt aaaaccttgc ttaattcctg cttcggcatt
     1501 ccagcttgat tcaggttgta cctctggatt tgggaaaaca gaaattccac cggcaccggt
     1561 ttcaatatat ttttctgcaa tggttggata acgatatcct tgtccgtatg agcaacgaat
     1621 aaaagttcct tctgtaagct gataatttaa tcctgcacgt aaaataggtt ctaactgcga
     1681 ttctttattg ttcatctcaa agtattcagt tctgaaacct gcagacacca tcaatttttt
     1741 tataagtttt ttttctaatt gtataaaagc tgcatagttt tcaagggtgt ttttatcgct
     1801 tccactacct gcataaagtg atgcatgact gaatgattgc tggaaataaa ctccagaggt
     1861 tacatttaat cttcctaatg aaatcaactc gcggttaaaa cgatattcag ccagataaac
     1921 atttgtttta tttgattgat cgttgccatt gtcattgtcg gcataaaaaa tgcgtgaacg
     1981 cagttgatgt gatgaacctt tttttccggt cagactgatg tatgggtcaa tataaaatgt
     2041 tttagaatcg gtaagtgtca ttgttttcgg gaaggcattg taaattcctg tgctgtcatt
     2101 tgcccaaacc aatgaaaagt tagagtgtga ctgcataaaa tttccattga tacccgctga
     2161 aaggccttcg acatttttaa acctgtgacg gatgttgaaa tttaatctgc cccagcgtga
     2221 tgcaacatca ttgttactga tggttgtatc ctgaaaaggt aaagaactca tagctaaagg
     2281 cgggccaata aatccgtggt catacaatcc attgccacca atgattaaat cccaacgatt
     2341 gattttctca ctatgtaaaa agttgatgcc tgaaaaagtt gcagggccat cccaccattt
     2401 tgcatctctg tttgatggtt tgtcataaac gccaccataa gttcttaaat aggtgtgata
     2461 acgctctttt ggataggctg tcctgaaatt gattgttcca cttaatgcag acgaaccata
     2521 taaaactgaa gaagcacctt taattacttc tatctgctca atattttcga caggaataaa
     2581 ttgccattct gtacgtcctg catcaccggt cataatggga aggccgtcaa tgagtgtagc
     2641 tactcgactg ccaacaccaa aactaaaacc actaccaccg cgaatttgtg gctcctgatc
     2701 taaaattgta acacccggag tttgttcgag tacactagta acattggttg tgtttctgtt
     2761 ttcaataagt tttggactga taacttccat gcttactgta atatcttcaa tacgttgttc
     2821 gtacttactt gcagaaacaa ctactacatt cagattttta aggtttgatg taagatagat
     2881 attgcggata gactctgtac catctttaat atctaatgta agggtatcat tttttaatcc
     2941 tgtcaatgta taaacgatgg tttgttttcc aacagggaga tttatctcat aacttccatc
     3001 agcatcagat gccacaccta ttgatttatc attgaccgga aaaataccgg cacctgcaat
     3061 aggttcacca ttttgtttta agtaaacatt tcctctcaac acacccattt gtgcatagca
     3121 ggaaatgcca aacaagacat ttgcaataat taatagaaat gatttttttg cgttattcag
     3181 atagcgcata ttaattcctg ataattactt ttccgttatt gataatgcct gaattatttt
     3241 cgatgatata aaaatacaaa ccatccaaag cagaaacatc tacagtactt tgttgtgagt
     3301 taagttctgt atctgttact attcgtccgg cagtatcaat taatttgaat tgataaacac
     3361 cggtattttt ataattaaat cgtatttttt cttttatgac attgcccaga atggaaactt
     3421 cgttttttgt atcaattgta tttacagaag aagggttaaa aaccattgca atatcatcaa
     3481 taaaaatctg actgttgaca ataggtccga ctcctttgct tgaggaaagt atccatatag
     3541 tgtttgatgg tgttgctgta gaaagataat ttacagcagc agcaaatcgt gtgtatgtac
     3601 ttactgtggc attaggtgtt tcaaatctta catagccaat agtgtctgtg tttgtagtgc
     3661 ctaacaattg caattcaaca aatccctgat ctgtaccggc aggtgtatat ttataccaac
     3721 ctgtaatact atccgggcga ccggtaaagg caatgcctcc tgatacgcca taaggtggtg
     3781 ttgtgattaa tgtgcctgta cttgctatac catttgctat aacattaaat ccgggaatta
     3841 atttagtagt taatttaatt gcatagcttc aactgtgtgc atctgcacct gaagcacgag
     3901 cacaagttaa cacacctatt attcc
//
LOCUS       DAORQT010000147         3907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4251_length_3907_cov_6.730270, whole genome shotgun sequence.
ACCESSION   DAORQT010000147 DAORQT010000000
VERSION     DAORQT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_3907_cov_6.730270"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..311
                     /locus_tag="PK134_05880"
     CDS             123..311
                     /locus_tag="PK134_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRB38412.1"
                     /translation="MQNGTVKFFNETKGFGFIKAENGEEIFVHSSGLKEQIRENDNVV
                     YEVQQGKKGLNAVNVRLA"
     gene            complement(536..1267)
                     /locus_tag="PK134_05885"
     CDS             complement(536..1267)
                     /locus_tag="PK134_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRB38413.1"
                     /translation="MIESLVIIPTYNEKENIQKIIKAVFNLLNTFHVLVIDDNSSDGT
                     ADLVKQLMPQFPESLHLVQRQGKMGLGTAYIHGFKWALERNYQYIFEMDADFSHNPAD
                     LQRLLDACKNGGADVAVGSRYINGVNVVNWPIGRVIMSYYASVYVRLITRMPVRDATA
                     GFKCYRRKVLETLDLSKIKFMGYAFQIEMKFAAWCSGFRIIEVPIIFTDRTEGKSKMS
                     SGIFKEAFFGVIQMKIKSWFRKYTH"
     gene            complement(1350..>1519)
                     /locus_tag="PK134_05890"
     CDS             complement(1350..>1519)
                     /locus_tag="PK134_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRB38414.1"
                     /translation="IKVILVNLSSMVFTINNGERIAQMVVAAHQQVVWKETLELSETN
                     RGAGGFGSTGK"
     assembly_gap    1520..1529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1530..1801)
                     /gene="dut"
                     /locus_tag="PK134_05895"
     CDS             complement(<1530..1801)
                     /gene="dut"
                     /locus_tag="PK134_05895"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRB38415.1"
                     /translation="MQNKTEVKIINKSSHPLPAYESNSAAGMDLRADLKEAVELKPLE
                     RALIPTGIYIELPQGFEAQIRPRSGLAAKHGVTVLNTPGTIDADYRG"
     gene            complement(1853..3376)
                     /locus_tag="PK134_05900"
     CDS             complement(1853..3376)
                     /locus_tag="PK134_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRB38416.1"
                     /translation="MSGIKKLAGQTVLYGLSSIVGRMLNYLLVPIYTRIFSPAEYGVV
                     SELYSYSAFLLILFTYGMETAFFNFMQKEENKNKVYENCISLVSYTTTILVLLMIFFS
                     SPLAATIEYPGKGNYITWLALIIGFDAITAIPFARLRQQGKALQFALLKIAGIVINIG
                     LNLFFLIGCPIWIKGNDGLLSQLAVTFYSPTVGVGYVFISNVIASGFTMLLLYRQFSN
                     LTWHLDSALIKRMIYYAFPLLIAGFAGMVNETFDRAVYKYLAPDANHALRDLGIYSAC
                     YKLSIIMTLFVQTFRYAAEPFFFAQHDKEDKKETYAKVMTWFVITCSFIFLGVMLFID
                     VIKLFIGEQFRSGIDVVPVLLLANLCLGVFYNLSMWYKLTEKTRYGAYFSIFGAVLTI
                     ILLFVLIPSFGYMGAAWATFITYFAMMIVSFITGQKHYPIPYKVKNDVFYVLLSLMLF
                     SLSAYLQHQLDLPGSVKWIVNIILLFVFPFVVFRMNKELLVKKQKVESQQNTIGRRN"
     gene            complement(3418..3687)
                     /locus_tag="PK134_05905"
     CDS             complement(3418..3687)
                     /locus_tag="PK134_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRB38417.1"
                     /translation="MIKTVAIEITGKVQGVFFRKFTQQTGRRLNLCGFVRNRSDGSVY
                     VEATGSELQLNELVAWCHKGSPMSLVRQVVVREISVIHNEPFQIL"
BASE COUNT         1355 a          745 c          604 g         1193 t
ORIGIN      
        1 gcacagatga tgaaaagcca tatctgaatg atataaataa attaatcaaa caaaacattg
       61 atgttgtaaa aacacatcca tataaataat tcaaactaaa gtaaaattaa attcaacaaa
      121 caatgcaaaa tggtacagta aagttcttta atgaaaccaa aggttttggc ttcattaaag
      181 cagaaaacgg agaagaaatt tttgttcact cttcaggttt aaaagaacaa attcgcgaaa
      241 acgacaatgt tgtttacgaa gtacaacaag gaaaaaaagg attaaatgca gtaaatgtaa
      301 gattagccta aaattccata ttgtaaaaaa gtaaaaaagg ctatctcatt aaatgagata
      361 gccttttctt atgcagcaga acctgatata atactgataa aagagtaccc tcagcgttcg
      421 ttacaagata atatttcgtc ttgactcttt ataaaattta ttttactgtt tggacacgcc
      481 ttattttgaa catcatattc ctttcaattc gcaataaata atataaatga tcttattaat
      541 gagtgtattt acgaaaccag ctttttattt tcatctgaat aacgccaaag aaagcttctt
      601 taaaaatacc gctgctcatt tttgatttac cttctgttcg atcggtaaaa attataggaa
      661 cttcgataat tcggaagccg ctgcaccagg cagcaaattt catttctatc tgaaatgcat
      721 atcccataaa tttaatcttt gacaaatcca gtgtttcaag cactttacgc ctgtaacatt
      781 taaatcctgc tgtggcatca cgaacaggca tgcgtgtaat caatctaaca taaacactgg
      841 cataatacga catgataact ctacctatcg gccagtttac aacattcaca ccattgatat
      901 atcttgaacc tactgctaca tcggcaccac catttttaca ggcatcgagc aatcgttgca
      961 agtcggcagg attgtgagag aaatcggcat ccatttcaaa aatatactga tagttgcgct
     1021 ctaatgccca cttgaaacca tgtatataag cagtgcctaa acccatcttt ccctgccgtt
     1081 gaaccaaatg caatgactcc ggaaactgag gcataagttg tttcaccaaa tcggcagtac
     1141 cgtctgatga attgtcatca ataaccaata catgaaaagt attcaacaga ttaaaaacag
     1201 ctttaataat cttctgaata ttttcttttt cgttatatgt cggtatgata actaatgatt
     1261 cgatcacaca attttatttg agttcaaaac tattattctc tgaaatgagt aattataata
     1321 atatgttaat tgataaaact attcatcaat tatttacctg tactgccaaa accaccagca
     1381 ccacgattgg tttctgacaa ctccagtgtc tctttccaca ctacttgttg atgtgctgcc
     1441 acaaccatct gcgctattct ttcgccatta tttattgtaa ataccattga tgacagattt
     1501 actaatataa cttttatctn nnnnnnnnnc cacgatagtc ggcatcaata gtacccggag
     1561 tgtttaatac ggtaacacca tgctttgctg caagtccact tcttggacga atttgtgcct
     1621 caaaaccttg aggtaattca atataaattc ctgttggtat taaagcacgt tctaatggct
     1681 taagttctac ggcttctttc agatcagctc taaggtccat gcctgcagca ctgttgcttt
     1741 cataagcagg aagcggatgc gatgatttat ttataatctt tacttctgtt ttgttttgca
     1801 tttttaattc tttcgctatt tgcaaaaata ttgattcttg tcaaatatac actcagtttc
     1861 ttcttccaat tgtattctgc tgcgattcta ctttttgttt ttttaccagc agttctttat
     1921 tcattctgaa tacaacaaac ggaaaaacaa agagtaaaat tatatttaca atccatttaa
     1981 cagaaccagg taaatcaagt tgatgttgca gataggcaga aagtgaaaac agcattagag
     2041 atagcaaaac ataaaacaca tcgttcttaa ctttataggg aataggataa tgtttctgtc
     2101 cggtgatgaa agatacaatc atcattgcaa aataagtaat gaaagttgcc catgctgcac
     2161 ccatatatcc aaacgaagga ataagaacaa acaatagaat aatagtcagc actgctccga
     2221 aaattgaaaa ataggcgcca tagcgggttt tttctgtgag tttataccac atacttaaat
     2281 tgtaaaatac accaagacaa agattcgcca acaagagcac aggaaccaca tcaatacccg
     2341 acctgaattg ttcgccaata aaaagtttaa tgacgtcaat aaataacatt acacctaaaa
     2401 aaataaatga gcatgtgata acaaaccatg tcattacttt agcgtaagtt tcttttttat
     2461 cttctttatc atgctgcgca aagaaaaatg gctcggcagc atatcgaaat gtctgcacaa
     2521 acaatgtcat gatgatgcta agtttgtaac aagcgctgta aattcctaaa tcgcgcaaag
     2581 catgattggc atccggagct aagtatttat aaacagcacg gtcgaatgtt tcattaacca
     2641 tacctgcaaa tcctgcaata agcaacggaa atgcataata aatcattcgt tttattaaag
     2701 cactgtcgag gtgccaggtt aaattactga attgccggta gagtaataac atggtaaaac
     2761 cactggctat aacgtttgaa ataaatacat atcctacacc cactgttgga gaataaaaag
     2821 tgacagcaag ttgcgataac aaaccatcat ttcctttaat ccatatcgga cagccaataa
     2881 gaaaaaataa attcaaacct atatttatca ctattcctgc aatctttaat aaagcaaact
     2941 gtaatgcttt gccttgttgt cgtagtcttg caaatgggat ggcagtgata gcatcaaaac
     3001 caataatcaa tgcaagccag gtgatgtagt ttccttttcc gggatactca atagttgcag
     3061 ccaatggcga tgagaaaaat atcatcagca acaccagaat tgttgttgta taagaaacta
     3121 atgaaataca attttcataa accttatttt tattctcttc tttctgcatg aaattaaaaa
     3181 aagctgtttc catgccgtag gtaaacagta tcagaagaaa tgctgaatag gaataaagct
     3241 cggaaactac accatattct gccggagaaa aaatgcgggt ataaatagga acgagtaaat
     3301 aattaagcat acgcccaaca atgctactca gaccataaag taccgtttga ccggctaact
     3361 tctttattcc actcatgaat cggctaaagt agccgtttta taatttactt tcacttacta
     3421 cagaatttga aatggttcat tgtggatgac agaaatttct ctgacaacaa cctgcctgac
     3481 taaagacatg ggtgagcctt tatgacacca tgctaccaat tcgtttaact gcaattcgct
     3541 gcctgttgct tcaacataaa ccgaaccgtc agagcggtta cgaacaaaac cgcaaaggtt
     3601 caatctacga cctgtctgtt gtgtaaattt tctaaaaaac acaccttgca ctttacctgt
     3661 aatttcaatt gctacagtct ttatcattca gctattaaac aatgaattgc aatgttaaga
     3721 aatttgtttg cttcaaagat ttttagctta tccgcaaaat aatactattc acctacaatt
     3781 ttaaaacttt tacctattag acaaaaagcg aattatcaaa cattttagtt ttggcacgtt
     3841 aacttataaa ccataaatca tatagtatga aacgttcagt agtaatcatt ggtattttac
     3901 ttgttgt
//
LOCUS       DAORQT010000148         3887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4293_length_3887_cov_5.661795, whole genome shotgun sequence.
ACCESSION   DAORQT010000148 DAORQT010000000
VERSION     DAORQT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_3887_cov_5.661795"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3546
                     /locus_tag="PK134_05910"
     CDS             <1..>3546
                     /locus_tag="PK134_05910"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38418.1"
                     /translation="IQGSQIALITEGGIVGNIMIKSEPSLNIKPGQNGIFFLIPHSQP
                     SGSKLSGMFEGYALSQSLILFDETSNSATGPFENYSDINALRNQIINATGGVQRISSI
                     APQQAPPSVNKIMIPPTIASIVPATTTAGTFSTITINGNNFGATYVNGTSNVEFPDAN
                     TGGVSYISAPQNHIQSWSNTQIQVWVPTQGGSGLIRVTNNLGETTTSAISITINYNET
                     NVVSGSNYYQPDLINNNGTGGYTFRYNTTFNGNAAAVAAFERALQTWRCGTFVNFNRS
                     GTTATTCQALDGTNLVTFDGSCALPAGVLGISYSYYSACATGVWYLNENDLKFRTNGT
                     GGINWNYGPAATGGGNFDFESVCLHELGHSHQLGHTIVPVTVMNYAIGPNTDRRTLTP
                     ISETAGGNDIMSRSVVTNACGPTAMVALSAGSCAINAPIADFNGTPLTGCNTLNVTFT
                     DASINTPTSWTWSFPGGVPNTFVGQNPPVIFYAAPGNYAVTLTVSNAFGSNTLTKTNY
                     VQIATCPPPTPDFTANPTTVCQGNTVQFVDLSTNTPTSWSWTFPGGTPGTSTAQNPVI
                     TYATAGTYNVTLTSTNAYGSNTLTKTNYITVNNCPAPPVADFSASPTTLCSGGTVNFT
                     DLSTNTPTSWSWTFAGGTPLSSVAQNPTITYNTPGTYAVTLIATNGGGSNTKTVSGYI
                     TVNLCSAPVASFSGFPTTICEGQAVTYVDQSTNSPSSWAWTFTGGAPAASALQNPVIS
                     YATAGTYTVSLTATNAFGNNTFTRTNYINVVTCPSPGTGLIVNDGSLIFVQPTAMMHV
                     EGGVINQDNLSNIGTWDNSGQVRIQGDWTNNSSGNAFINSSPGEVELFGANQTIRGST
                     PTFFFTLNLTGTGIKTQNIDATVQGLLKLNDRELATQGNVMHVTNTAPAAITRTGGLN
                     STPVQGFVSSTGSGKLWRNTNTTSVYLFPVGSSIPPARFRPVEIKPLSATPNTFAVRM
                     VNNDPNTDGFNRAVREPTLGAVNAGWYQKINRVSGSSLAEFSLYADDAQDAIASWPLL
                     KLCQWGQAAPPVWWKDIGGAVTPGASPVLGSVLRTNWNNFSNTENFNIAPESNPLPVE
                     LLSFTGNCKNNIVNLNWTTASEINNHYFELQQSADGNDFVTIGRVEGNGTTIKQNFYS
                     FTIDQKTEHTFFRLVQYDFDGRANVLPAILVSCNTE"
     assembly_gap    3547..3573
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3574..3791
                     /locus_tag="PK134_05915"
     CDS             <3574..3791
                     /locus_tag="PK134_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38419.1"
                     /translation="VFVSDIYLPEKTQLSMKVFNTIGQLVMEEQWTLDAGYHKQATQV
                     STLAKDIYNIRYTSNSFNIVKQIAIVK"
BASE COUNT         1177 a          812 c          771 g         1100 t
ORIGIN      
        1 attcagggca gtcagattgc attgattaca gaaggcggta ttgttggtaa catcatgatt
       61 aagtcagaac catctttgaa tattaaaccc ggacagaatg gaatattttt tctaattcca
      121 cacagccagc cgtcaggaag taaactgtca ggaatgtttg aaggatatgc tttgagtcag
      181 agtttaattt tgtttgatga aacaagtaat tcagcaacag gtccgtttga aaactacagt
      241 gacatcaatg cattacgaaa tcagattatc aatgctacag gtggcgttca gcgcatttct
      301 tctattgcac cacagcaggc acctccatct gtaaataaaa taatgattcc gccaactatt
      361 gcttcaatag taccggcaac aactacggca ggaacatttt caacaatcac aataaacgga
      421 aataattttg gtgccaccta tgttaacgga acttcaaacg ttgaatttcc tgatgccaat
      481 actggaggtg taagttatat ttctgctccg caaaatcata ttcagtcgtg gagcaacaca
      541 caaattcagg tttgggtacc tacacaaggt ggaagcggat taataagagt aacaaacaat
      601 cttggagaaa ctacaacttc tgcaatttca attaccatta attataatga aacaaatgtt
      661 gttagtggta gtaattatta tcaacccgac ttgattaaca ataatggaac cggaggttat
      721 acttttcgtt acaacacaac atttaatggt aatgctgctg ctgttgctgc atttgaaaga
      781 gcattacaaa catggcgctg tggaactttt gtaaacttca accgctcggg tactactgca
      841 acaacttgtc aggctttaga cggaactaac ttagtgacct ttgatggttc atgtgcattg
      901 cctgccggag ttctcggaat ttcgtacagt tattacagtg catgcgcaac aggcgtttgg
      961 tatcttaacg aaaacgattt aaagtttcgt accaatggaa ccggaggcat aaactggaac
     1021 tatggccctg ctgcaaccgg tggtggcaat ttcgattttg aatcagtttg tttgcatgaa
     1081 ttaggacaca gtcatcagtt aggtcacact attgtgcctg tgacagtaat gaattatgct
     1141 attggcccaa atacagacag acgtacactt acaccaatca gtgaaactgc cggtggaaat
     1201 gatataatga gtcgtagtgt ggtgactaat gcctgcggac caacagctat ggttgcgctt
     1261 tctgcgggaa gctgtgccat caatgcacct attgctgatt ttaatggtac accactgaca
     1321 ggatgcaata cgctgaacgt aacttttaca gatgcatcca tcaatactcc aacatcgtgg
     1381 acgtggtctt ttccaggagg ggtaccaaat acttttgtag gacagaatcc tcctgtaatt
     1441 ttttatgccg caccgggcaa ctacgcagtt accctaactg taagcaatgc atttggaagc
     1501 aatacactta ctaaaacaaa ttatgtacag attgcaacct gccctccacc tacacctgat
     1561 tttacagcta atccaacaac cgtttgtcag ggaaatactg tacagtttgt agatttgagt
     1621 accaacacac caacaagctg gagttggact tttccgggag gtacaccggg aacatctact
     1681 gcacaaaacc ctgtgattac ttatgccact gcaggaactt ataatgttac attaacctca
     1741 actaatgcct atggcagcaa cacattaaca aaaacaaatt acataacagt aaacaattgc
     1801 cctgctccac cggtcgctga ttttagtgca tcgccaacaa cactgtgttc cggtggtaca
     1861 gtaaacttca ccgatctttc aactaacaca cccacatcat ggtcgtggac atttgccggt
     1921 ggcacaccgt tatcatctgt agcacaaaat ccaacaataa cgtataacac acccggcact
     1981 tacgcagtta cattaattgc aactaatggt ggtggctcga acacaaaaac tgtatcagga
     2041 tatattacgg taaatctttg ctctgcacct gttgcatctt tttcaggatt tcctacaacc
     2101 atctgcgaag gtcaggctgt gacttatgta gatcagtcaa ccaactcacc atcatcatgg
     2161 gcatggactt ttaccggtgg tgcacctgca gcatctgcct tgcagaatcc tgttattagc
     2221 tatgctactg caggcaccta cactgtttca ttaactgcaa caaatgcttt tggcaacaac
     2281 acttttacta gaacaaatta catcaatgtt gttacatgtc cgtcacctgg aacaggactt
     2341 attgtcaatg acgggtcact gatttttgtt caacctactg caatgatgca tgttgaaggt
     2401 ggtgttatta atcaggataa cctgagcaac attggtacat gggataactc cggtcaggtg
     2461 cgtatacaag gcgactggac aaacaattca tctggaaatg cttttatcaa cagcagcccg
     2521 ggtgaagtgg aactgtttgg cgccaatcaa actataagag gtagtacacc tactttcttc
     2581 tttaccttaa atctgacagg cacaggtata aaaacccaaa atattgatgc aacggttcag
     2641 ggattgctga agttgaacga cagagaactt gccacacaag gaaatgtgat gcatgttacc
     2701 aatacagcac cggcagctat tacgcgtaca ggtggactta actcaactcc tgttcagggt
     2761 tttgtgagta gcacaggcag tggtaagttg tggcgaaaca ccaacactac atctgtgtat
     2821 ttatttcctg taggttcttc aatacctccc gcacgttttc gtccagttga aataaagcca
     2881 ttgagtgcaa cacccaatac ttttgctgta cgtatggtta ataatgaccc gaatactgat
     2941 ggttttaacc gtgctgtacg tgaacccacc ttaggtgcag taaatgcagg atggtatcag
     3001 aaaataaaca gagtttccgg tagttcgtta gcggagtttt cgctttatgc cgatgatgca
     3061 caggatgcca ttgcttcatg gccattactc aaactttgtc agtggggaca ggctgcccct
     3121 cctgtatggt ggaaagatat tggcggtgct gtaactccgg gcgcttcacc tgtgttagga
     3181 tcggtgctgc gtaccaactg gaataatttc agtaatacag aaaatttcaa tattgcacca
     3241 gaatcaaatc cactgcctgt tgaattactt tcgtttaccg gtaactgtaa gaacaacatt
     3301 gttaatctga attggacaac tgcatctgaa ataaacaatc attattttga attgcaacaa
     3361 tcagccgatg gaaatgattt tgttactata ggtcgtgttg aaggtaatgg taccaccatc
     3421 aaacaaaatt tttattcttt taccattgat caaaaaactg aacacacatt tttccgattg
     3481 gtgcaatatg attttgatgg aagagctaat gtattgccgg ctattttagt ttcgtgtaac
     3541 acagaannnn nnnnnnnnnn nnnnnnnnnn nnnatgtttt tgtttctgac atatatcttc
     3601 ctgaaaaaac acaactctcc atgaaggtat ttaataccat cggacaattg gttatggaag
     3661 aacaatggac acttgatgca ggctatcata aacaagcaac acaggtttct actcttgcaa
     3721 aagacattta caatatccgc tatacttcaa actcgtttaa catagtaaaa caaatagcta
     3781 ttgtaaaata attgctgatg tcagaaattg tattgcgtag tttgcaaatt gacgatgcag
     3841 aaatatttgc tttgcatgcc aacaacagtg caattgcaga taacatg
//
LOCUS       DAORQT010000149         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4348_length_3847_cov_9.331224, whole genome shotgun sequence.
ACCESSION   DAORQT010000149 DAORQT010000000
VERSION     DAORQT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_3847_cov_9.331224"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /gene="porU"
                     /locus_tag="PK134_05920"
     CDS             <1..1534
                     /gene="porU"
                     /locus_tag="PK134_05920"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRB38420.1"
                     /translation="RYPDVNDAIDKRVNKGSLIINYVGHGGETGWAEERVLTNDDINS
                     WTNINKLPVFITATCEFSRWDDPARTSSGEKILLSPTGGGVALFSTTRLVFASSNEVL
                     NRALIKFMFDDPNYRLGDIMVASKTDPGNLNLNTRNFSLLGDPALRLHYPKFNIEATQ
                     INAQPIIALNDTLSSLEKVTIAGRIVKGGQLQSDFNGFLYPTVYDKASSITMLRNDAS
                     SPNITFKLQKNILYKGKATVKNGEWSFTFIVPKDISYQFGLGKLSFYAENGVEDGAGY
                     FDSIVVGGSSANIISETDGPGIKLYLNDERFAFGGTTNEKPTIVCLLSDSTGINTVGN
                     GVGHDITAIIDGKVDPVYVLNDYYEADLDSYSSGRVAYPLSKLPEGRHTLKVKAWDIL
                     NNSSEAYTEFIVASDASLALKHVLNYPNPFTTHTQFSFEYNKPCEDLEVQVQIFTIAG
                     KLVKTISKKIITPGTRIDDLTWDGRDDFGDRIGRGVYIYRVKLNTADESASETQKLVI
                     LK"
     gene            1661..2863
                     /gene="porV"
                     /locus_tag="PK134_05925"
     CDS             1661..2863
                     /gene="porV"
                     /locus_tag="PK134_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRB38421.1"
                     /translation="MRYKLFYGALSLAVLSVQNQVSAQTDKANLLGQINTITTAVPFL
                     MISPDARAGGMGDAGVASSADVNSIHWNPAKLVFADKKMGVGISYTPWLRKLVPDINL
                     AYVSFFTKPNDVSAIGASLRYFSLGNIDFTSETGDPMGSYKPNEFAVDVAYSRKLGDE
                     FSGGIALRYIHSSLTGGITVGGNATKAGNSVAADVSFFYKTNLEISGKDAEFGAGLAI
                     TNIGSKMSYTDKGTKDFIPINLRLGPSLKIKLNDYNELAFLVDFNKLLVPTPPVYSDS
                     TIAGEENPIIFAGKDPNVGVAQGIFQSFGDAPGGGKEELREINIAGGLEYWYDKQFAF
                     RAGYFYENKTKGNRKYFTLGIGLRYNIFGLDFAYLIPTEQRNPLENTLRFSLSFNFDS
                     IKGSDSNE"
     gene            2867..3346
                     /gene="ispF"
                     /locus_tag="PK134_05930"
     CDS             2867..3346
                     /gene="ispF"
                     /locus_tag="PK134_05930"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRB38422.1"
                     /translation="MNFRVGFGFDVHQLEQGIPFFLGGVRIEHYKGAKGHSDADVIIH
                     AICDALLGAAALDDIGKHFPDTSNEFKGIDSSILLAKVVSLLKEKSIAIGNVDVSVVL
                     EKPKIAPYIQAMREKLASVMQIEIDCVSIKATTNEKMGFVGREEGVAAYAVALINRQ"
     gene            complement(3333..3740)
                     /locus_tag="PK134_05935"
     CDS             complement(3333..3740)
                     /locus_tag="PK134_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRB38423.1"
                     /translation="MDNRHDIITPDDITLMVDTFYSAIREDDLLGPIFNNIIQDRWPE
                     HLKKMYGFWRSILFNEPLYNGSAFEAHRQLPIEMKHFKHWLKLFEATVDGLFEGSNAE
                     TAKSRARKIAEVFYYKIESERGNEPQNFSPTAG"
BASE COUNT         1177 a          640 c          770 g         1260 t
ORIGIN      
        1 gcgttatcct gatgttaatg acgctattga taaacgtgtc aataaaggtt cgttgattat
       61 taattatgta ggtcatggtg gcgaaacagg atgggcagaa gagcgagttc ttaccaatga
      121 tgatattaac tcatggacaa acattaataa actgccggta tttattacag caacctgtga
      181 gtttagcaga tgggacgatc ctgcaagaac atcatctggc gaaaaaatat tattgagccc
      241 aaccggtgga ggtgttgcat tgttttcaac tacacgactg gtatttgcaa gcagcaacga
      301 agttcttaac agagccttga taaagtttat gtttgatgat ccaaattatc gtttaggcga
      361 tatcatggtt gccagtaaaa ccgatccggg aaatttgaat ctgaatacac gtaattttag
      421 tttactcggt gatccggcat taagattaca ttatcctaaa ttcaatattg aggctacaca
      481 aattaacgct caaccaatta tagcgctaaa cgatacttta agctcattag agaaagtaac
      541 cattgcagga agaatagtta aaggtggaca acttcagtct gattttaatg ggtttttata
      601 tccaaccgta tatgataaag catcaagcat aaccatgttg cgcaatgatg catcaagtcc
      661 taacataact tttaagcttc agaaaaatat tttatataaa ggaaaagcta cagtaaaaaa
      721 tggtgaatgg tcgtttacat ttattgtacc caaagatatt tcttatcagt ttgggttagg
      781 caagctaagc ttttatgctg aaaatggagt agaagatggt gcaggatatt ttgatagcat
      841 agttgtgggt ggttcgtctg caaatattat ttctgagaca gatggacccg gtataaaact
      901 ataccttaac gatgagcgct ttgcttttgg cggcactact aatgaaaaac ctacaattgt
      961 atgtttgctc tctgactcaa caggtatcaa tacagttggt aatggtgttg ggcatgacat
     1021 cacagcaata attgatggaa aagttgatcc cgtttacgta ctcaatgatt attatgaagc
     1081 ggacttagac agttattcta gtgggagagt tgcttatccg ctatcaaaac ttccagaagg
     1141 aaggcatacg cttaaagtaa aggcatggga tattctcaac aactcaagtg aagcttatac
     1201 agagtttatt gtggcttctg atgcatcatt agcactaaag catgttttaa attatcccaa
     1261 cccgtttaca acgcatacgc agttttcatt tgaatacaac aagccttgtg aagatttaga
     1321 agttcaggtt cagattttta ccattgccgg taaattggtt aaaaccattt ctaaaaagat
     1381 aattaccccc ggtacaagaa ttgatgattt aacatgggat ggtcgtgatg attttggcga
     1441 cagaattggt cgtggagtat atatttacag agtaaaactc aatacagcag atgagtcggc
     1501 ttcagaaacg caaaaactgg tgattctcaa atagaaacct tttttatttt ttatcgtata
     1561 tttgccagcc gcttcttaaa aataacatca taaaataaaa accacttatt gtcgttattc
     1621 aaacttagct tttaaaactt attactgaat ttctttatac atgagatata aattgtttta
     1681 cggtgctttg tctttagcag tactttcagt acaaaatcaa gtgtctgcac agactgataa
     1741 agccaactta cttggtcaga ttaacactat tacaactgct gttccgtttc tgatgatttc
     1801 tcccgatgcc cgtgccggtg gtatgggcga tgccggtgtt gcatcatctg cagacgttaa
     1861 cagtattcat tggaatcctg ccaaactggt gtttgcagac aaaaagatgg gtgttggaat
     1921 ttcttacaca ccttggttac gaaaattagt gccggatata aatctggctt acgtaagctt
     1981 ttttaccaag cctaatgatg tttctgcaat tggagcttcg ttacgttatt tctctttagg
     2041 taatattgat tttacttctg aaacaggcga ccctatggga tcgtacaagc ccaatgagtt
     2101 tgctgttgac gttgcatact caagaaagtt gggtgatgag tttagcggag gtattgcttt
     2161 gcgttatatc cactcaagtt taacaggtgg aattactgtt ggtggcaatg ctactaaagc
     2221 aggaaattct gtagctgccg atgtttcttt cttctataaa acaaatcttg aaatttcagg
     2281 aaaagatgca gagtttggcg caggtttggc cattacaaat attggttcaa aaatgtcata
     2341 taccgataaa ggaacaaagg attttattcc tattaatctg cgcttaggac cttctttgaa
     2401 aataaaactc aatgactata atgaattagc atttttagtt gatttcaata agctattagt
     2461 gccaactcca ccggtttatt ccgattctac tattgcaggt gaagaaaatc ctattatttt
     2521 cgcagggaaa gatcctaatg ttggtgtagc acaaggaatt tttcaatcat tcggtgatgc
     2581 gccaggagga ggaaaagaag aattgcgcga aattaatatt gcaggaggat tggaatattg
     2641 gtacgataag caatttgcat tccgtgcagg atatttctac gagaacaaaa ctaaaggaaa
     2701 cagaaagtat tttactttag gtataggttt acgctacaac attttcggac ttgattttgc
     2761 ttatcttatt ccaacagagc agcgcaatcc attagaaaat actttacgtt ttagcttgtc
     2821 attcaacttt gattcaatca aaggaagcga cagtaacgaa taaacggtga atttcagagt
     2881 tggttttggt tttgatgttc atcagttaga acagggcatt cctttttttt taggcggagt
     2941 tcgaattgaa cattataaag gtgccaaagg acatagtgat gccgatgtaa ttatacatgc
     3001 catttgcgat gccttgcttg gagcagcagc acttgatgat attggtaaac attttccgga
     3061 tacatccaac gaatttaaag gaatagattc ctcaatttta ttagcaaaag ttgtttcatt
     3121 attaaaggaa aaatcaattg ctattggtaa tgttgatgtg agtgtagttt tggagaaacc
     3181 taaaattgca ccgtatattc aggcaatgcg ggagaaatta gcttctgtaa tgcagattga
     3241 aatagactgt gtttcaatta aggcaactac caacgaaaaa atgggttttg ttggtcgtga
     3301 agaaggtgtt gctgcttatg cagttgcctt gattaaccgg cagtaggact aaaattctgt
     3361 ggctcattgc cacgttctga ttcaatttta tagtaaaaga cttctgcaat ttttcttgct
     3421 cttgattttg cggtttctgc attgctgcct tcaaacaaac catctactgt agcttcaaat
     3481 agtttaagcc agtgtttaaa atgcttcatt tctattggca attgcctgtg ggcttcaaat
     3541 gcacttccat tgtataacgg ttcgttgaag agaatgctac gccagaaacc atacattttt
     3601 ttcagatgtt ctggccagcg atcttgaatg atgttgttga atattggacc taacaaatca
     3661 tcttcacgaa tagcactgta aaatgtatca accattaatg ttatatcatc aggtgtaatt
     3721 atgtcatgtc tattatccat cctcattttt tatttttttt ctatttcaac ttctctgatt
     3781 tcacccgata ttatttttat ttttttggct gatggttttt ttaagaatga attgaagata
     3841 ctttgtg
//
LOCUS       DAORQT010000150         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4350_length_3847_cov_7.105749, whole genome shotgun sequence.
ACCESSION   DAORQT010000150 DAORQT010000000
VERSION     DAORQT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4350_length_3847_cov_7.105749"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..>374
                     /locus_tag="PK134_05940"
     CDS             180..>374
                     /locus_tag="PK134_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38424.1"
                     /translation="MALSRIWSYMILFAVAVAGYRYMQGDKNIFNDMVVEKKLAAGES
                     FSKVSYYVIGQGIVSVLKEPK"
     assembly_gap    375..384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            545..1858
                     /locus_tag="PK134_05945"
     CDS             545..1858
                     /locus_tag="PK134_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRB38425.1"
                     /translation="MIYSEELITDSTLNITNNLLQANVVLTSEVNTDSLRNAWTAISN
                     RQKIVGSNEVFLWLPKNIDGIFETGKFAVTIAIGLIGTLALFMGLLNIAERAGGINII
                     SRLISPFLSKLFPSLPKNHPSFGHMVMNFSANMLGLDNAATPFGLKAMQSLQEINTNK
                     DRASDAQIMFLALHASGLTLIPVSIIAVRSANGSHNPNEIFIPLMLTTFVCTLTAMIV
                     TSLKQKINLFQRGLLLPVAGLSLTLSLFVWFLSSLRPFALDQFSDAFSNGAILLVFFG
                     IIAGGLYKKINLFDAFVDGAKGGFETAVRIIPYMVGMLVAVSMLRTSGAFDLIINPVK
                     HLIAISGMDTRWAEGLPTALLRPFSAGGARGFMIDAMRTFGPDSFIGKLVSVLQGSSE
                     TTFYVVALYFGSVSVKDTRYAIPVMLLADLAGVIFSIVICYLLWA"
     gene            1998..>3847
                     /locus_tag="PK134_05950"
     CDS             1998..>3847
                     /locus_tag="PK134_05950"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRB38426.1"
                     /translation="MSQIVYVVPVLGLLALVYMFIKASWVGKQDAGEKNMQELAGHIA
                     DGAMAFLKAEWKILSYFVVIAGLLLAYSGTLHGTEEYPVHSSPIIAVAFVIGAVFSAV
                     AGYIGMNIATKANVRTAQAARSSLSKALKVSFTGGAVMGLGVAGLAVLGLGSLFIVFY
                     QLFVPTGAAITGVEMKKAIEVLAGFSLGAESIALFARVGGGIYTKAADVGADLVGKVE
                     AGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSYVATILATMVLGQEIDASADKFG
                     GLSPILLPMLIAGMGIIFSIVGTWFVRIKSENDNVQNALNMGNWSSIVLTAIASYFAV
                     TYLLPEKLVIRGFEFTPMNVYLAILVGLVVGALMSIITEYYTAIGKRPVLSIVKQSST
                     GHATNIIGGLAVGMESTAVPMIVLAGGIFLSYSLAGLYGVAIAAAGMMATTAMQLAID
                     AFGPIADNAGGIAEMSQLPKEVRQRTDNLDAVGNTTAATGKGFAIASAALTSLALFAA
                     FVGVAGISHIDIYKANVLAGLFVGAMIPFIFSSLAIAAVGRAAMSMVEEVRRQFREIP
                     GIMEYKAKPEYEKCVAISTEASIREMIAPGALALIVPVVVGFIFGPEVLGG"
BASE COUNT         1056 a          668 c          854 g         1259 t
ORIGIN      
        1 tatcagcatg gggtgctgga ggtcgctggt tcgaatccag tcaccccgac agttttcatc
       61 tcaaagcgtt ctgtaaaaca gggcgctttt tcttttcaca cataatttgt ggatattatt
      121 cagcacctta tgtaaattac ttatcacagt catttatttt tcagtccgaa atttaacaca
      181 tggcattaag cagaatttgg tcgtatatga ttttatttgc tgtagcagta gcaggctatc
      241 gttacatgca aggtgacaaa aatattttta atgacatggt ggttgaaaaa aaattagctg
      301 ccggtgaaag tttcagtaaa gtatcttatt atgtcattgg tcagggaatt gtcagtgttc
      361 tcaaagaacc aaaannnnnn nnnntgccaa ttgggtaaag acagataaat tctcagatgc
      421 tcaggtaata atcaccactg ctgatttaaa gaataatctg cgcgagcaat taaataatca
      481 acttgcaaaa atcagttttg cgaatattaa cactcaaaac aaattcactg ctgacaaatc
      541 gaaattgatt tattctgagg agttaattac tgacagtaca ttaaatataa caaacaatct
      601 gcttcaggct aatgttgttc ttacatctga agtaaataca gattcgcttc gaaatgcatg
      661 gacagctatc agcaacagac aaaaaattgt tggtagcaat gaagtatttc tctggcttcc
      721 taaaaacatt gatggtattt ttgaaacagg aaaatttgca gtgactattg ccataggtct
      781 tataggtacg ctggcattat ttatgggctt actcaatatt gctgaacgtg caggtggcat
      841 caacatcatc agccgattaa taagtccgtt tttgtcgaag ctttttcctt cgttgccaaa
      901 aaatcatcca tcgtttggac acatggtgat gaacttttct gctaatatgc ttggcctcga
      961 caatgcggct acaccttttg ggcttaaggc tatgcagagt ttgcaagaaa ttaataccaa
     1021 caaagacagg gcaagcgatg cacagattat gtttctggca ttacatgcat cagggttaac
     1081 actgattcct gtttctataa ttgctgttcg ttctgctaat ggatcgcaca atcctaatga
     1141 gatttttatt ccgctcatgc ttacaacttt tgtttgtact ctcacagcta tgattgtaac
     1201 ctcgctcaaa caaaaaatta atttgttcca acgtggattg ttgttacctg ttgccggact
     1261 gagtttaaca ttaagtttgt ttgtatggtt cttaagcagc ctgcgaccat ttgcattaga
     1321 tcaattttct gatgcattca gcaatggtgc tattttattg gtgttttttg gaattattgc
     1381 aggaggactt tataaaaaaa taaatctctt tgatgcattc gttgatggtg cgaagggagg
     1441 ttttgaaaca gcagttcgta ttattcctta catggtagga atgcttgttg cggtgagtat
     1501 gctcaggaca agtggtgcgt ttgatttaat cattaatcct gtaaaacatc ttattgcaat
     1561 ttctggcatg gacacacgtt gggcagaagg tttaccgaca gcattattac gacctttcag
     1621 tgcaggtggt gcaagaggat ttatgataga tgccatgcgc acatttggtc ctgattcgtt
     1681 tattggtaag ttagtgagcg ttttgcaagg ctcgagtgaa acgacatttt atgttgttgc
     1741 tttgtatttt ggctctgttt ctgtaaaaga tacacgttat gctattcctg ttatgcttct
     1801 ggccgacctg gccggagtaa tattctcaat cgtaatctgc tacttgttat gggcataatc
     1861 taacagttta gttgttagcg agttacatac ataaaaatcc ggtttaattt aagttgattt
     1921 taaggccaga aataggggcc tactaatcaa gtttttttat actttcgcaa cctcaaaaat
     1981 tttaaccgta gtattttatg agtcagatag tttatgtagt tccggtgttg ggtttgttgg
     2041 ccctggttta tatgtttata aaagcctcgt gggtaggtaa gcaggatgca ggtgaaaaga
     2101 acatgcaaga acttgcaggt cacattgcag atggagccat ggcattttta aaagcagaat
     2161 ggaaaatatt atcctatttt gttgttatag caggattgct attggcttat tcaggaacac
     2221 tacatggcac agaagaatat cctgttcatt caagcccgat aatagctgtt gcctttgtga
     2281 taggtgcagt tttttctgca gtagcaggct atattggaat gaacattgcc actaaagcaa
     2341 atgtccgtac agcacaggca gcaagaagca gcctttcaaa agcattaaaa gtttctttca
     2401 ccggaggtgc agtaatggga cttggtgttg caggccttgc agtactagga ttaggttcgt
     2461 tatttattgt attctatcaa ttgtttgttc ccacaggtgc agcaattacc ggtgttgaaa
     2521 tgaaaaaagc tattgaagta ttagcaggtt tttctctcgg tgcagaaagt attgctttat
     2581 ttgctcgtgt tggcggaggt atttatacta aagctgccga tgtaggtgct gacttagttg
     2641 gaaaagttga agccggtatt cctgaagatg atccgcgtaa ccccgctaca attgcagata
     2701 acgttggcga caatgttggt gatgttgccg gtatgggtgc cgacttattc ggttcttatg
     2761 ttgcaactat tcttgctaca atggtattag gtcaggagat tgacgcttct gctgataagt
     2821 ttggcgggct ttcacctatt ttattaccaa tgcttattgc aggaatggga attatttttt
     2881 caattgtagg aacatggttt gtgagaatta aaagtgaaaa tgataatgtt cagaatgcat
     2941 tgaacatggg taactggtcg tcaattgtac ttacagcaat tgccagttat tttgccgtga
     3001 cttatttact tcctgaaaaa ttagttatcc gtggatttga gttcacacct atgaatgttt
     3061 atttagccat attggttggt cttgtagtgg gagcattaat gagcatcatc acagaatatt
     3121 atactgctat cggaaagcga cctgttttat caatcgttaa acagagctca accggtcatg
     3181 ccacaaacat tattggtgga ttggcagtag gtatggaatc aactgcagtg cctatgattg
     3241 ttcttgcagg aggtattttt ctttcctatt cattagcagg tctatatggc gttgccatag
     3301 cagcagcagg tatgatggca acaacagcta tgcagttggc tattgatgcc tttggtccta
     3361 ttgcagacaa cgcaggcggc attgccgaaa tgagtcagct tccaaaagaa gttcgtcagc
     3421 gcacagataa tcttgatgct gtaggaaaca caactgctgc aacaggaaaa ggttttgcca
     3481 ttgcatcggc agcattgaca tcattagcat tgtttgcagc ctttgttggt gttgcaggca
     3541 tttcgcatat tgacatttat aaagccaacg tgcttgcagg cttgtttgtt ggtgccatga
     3601 ttccttttat attttcttcg ttggctattg ctgccgtagg tcgtgctgca atgagcatgg
     3661 tagaagaggt gcgcagacag tttcgcgaaa ttccgggcat catggagtac aaagcaaagc
     3721 ctgagtacga aaaatgtgtt gctatctcta cagaagcttc tatcagagaa atgattgctc
     3781 cgggtgcatt agcattaatt gtacctgttg tagttggatt tattttcgga cccgaagtgc
     3841 ttggtgg
//
LOCUS       DAORQT010000151         3843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4358_length_3843_cov_5.893611, whole genome shotgun sequence.
ACCESSION   DAORQT010000151 DAORQT010000000
VERSION     DAORQT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_3843_cov_5.893611"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PK134_05955"
     CDS             <1..1061
                     /locus_tag="PK134_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HRB38427.1"
                     /translation="SLAKTMEVKFTVSGPPIGGAKSGINFDPADPRKNQVLERWFKAI
                     KPLLKTYYGTGGDLNVDEIHDVIPICNKLGILHPQEGTAVGHFNDGETNIKNRISNLQ
                     QGVSLVLKDASLSPDLSHKYRVADMITGYGVASAVKYYYELWGGKISEKRAAIQGWGN
                     VAAAAGFYLGQMGVKIVGIIDRDGGLINENGYTFEEIKTLFNNRQGNKLVADNMLSPQ
                     QMAEKVWNLDAEIFIPGAASRLVTQEQMQSLINKKLEVISCGANVPFADKEIFFGPIG
                     LYCDKHVSVIPDFIANCGMARVFAYLHNANAVIDDRLIFDDAATTIKNALQEVIATHK
                     DKKGIASAAFEIALKKLL"
     gene            1061..2176
                     /locus_tag="PK134_05960"
     CDS             1061..2176
                     /locus_tag="PK134_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB38428.1"
                     /translation="MLIDVLHQRILGNTIEDYLWFAGLILAGLVFKNLISRILTFLLF
                     NVLKKYFKTIGVNKFVELTQKPFSATLFLIIIYIACRYVHFPDEWKLASESEFGVRMT
                     IKLIFQIVTVFFITWTILRVIDYIGLVMRERANKTESKMDDLFVPFFASSMKAITVII
                     SLFFILGAVFKLNVASIIAGLGIGGLAIALASKDTLENLLGSFLIFMDKPFVVGDLVK
                     TDSLRGHIEEVGFRSTKVRTIEKTLVIVPNKKMMDAELENQTERTFYRHRFVIGLVYS
                     TTADQIQNIIQQILNMLNEHPLVNENPNVFFTNFGASALEIEVIYFVKTQELSDFENT
                     RQEVNFGVMKIVAENKSDFAFPSTSVYIEKEVKKFLQ"
     gene            complement(2251..2847)
                     /locus_tag="PK134_05965"
     CDS             complement(2251..2847)
                     /locus_tag="PK134_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38429.1"
                     /translation="MKKQIASLLFIAAVVSTASAQKIKILDGSLAGLKGETKLNVVYD
                     YSNMGVGSFAKEEEYVAKKTREYNAKEPGRGDSWAKSWVADRKNRFEPSFVELFNKKS
                     PMLIGDFPDAKYTMKVSTTFTEPGFNVYVTRKNASINLEITVYETANPDKPICKMQGL
                     RFPGRTFGGYDYDTGTRISESYAKAGKDLAAFIGKNLK"
     gene            complement(2994..>3843)
                     /locus_tag="PK134_05970"
     CDS             complement(2994..>3843)
                     /locus_tag="PK134_05970"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRB38430.1"
                     /translation="VMVVTDRVSAFDVVLPRPIPYKGAVLNQIAAHFMQATADLVPNW
                     IVDTPDVQVSIGIPCQPLRVEMVIRGYLSGHAWREYKQGKRMLCGVKLPDGLNESDAL
                     PEPIITPTTKAAHGHDQDISKEEIIKQGIVDAAIYEQAESYTKQLFRRGSEMAAQRGL
                     ILVDTKYEFGVHQGKVYLIDEIHTPDSSRYFYADGYAERQKRKQPQKQLSKEFLRQWL
                     IENNFQGLAGQTVPEMSDEIVQMVSDRYIELFENITGKTFVKPSNENIEQRIYGNIVN
                     SLEKLT"
BASE COUNT         1196 a          708 c          690 g         1249 t
ORIGIN      
        1 aatcgcttgc caaaacaatg gaagtgaagt ttactgtatc cggtccacca ataggaggag
       61 ctaaatccgg tattaacttc gaccctgctg accccagaaa aaatcaggtt cttgaacgtt
      121 ggtttaaggc cataaagcca ttgctgaaaa catattacgg aaccggtggc gatttgaatg
      181 ttgacgaaat acatgatgtt attccaattt gtaataaact tggaatactt catccgcagg
      241 aaggtactgc agttggtcac tttaacgatg gcgaaacaaa tattaaaaac cgtatcagca
      301 atttgcagca aggcgtttca ttggtattga aagacgcttc gctatcgcct gatttgagcc
      361 ataaatacag agttgccgat atgattaccg gttatggtgt tgcatcggcc gtaaaatatt
      421 actatgagtt atggggtgga aaaatttctg agaaaagagc tgccattcag ggatggggca
      481 atgttgctgc cgctgcagga ttttatcttg gacaaatggg cgtaaaaatt gtaggaatca
      541 ttgacagaga tggcggactg ataaacgaaa acggctatac gtttgaagaa ataaaaacat
      601 tattcaacaa ccgtcaggga aacaagctgg tggcagataa catgctttct cctcaacaaa
      661 tggcagaaaa agtgtggaac cttgatgcag aaatatttat tccgggtgct gcatctcgat
      721 tggttactca ggagcaaatg caatcgctca tcaacaaaaa acttgaagta atttcctgtg
      781 gtgccaatgt accttttgct gataaagaga ttttcttcgg ccccattgga ttgtattgtg
      841 acaaacatgt aagtgtaatt cccgatttta ttgctaactg tggaatggca agagtgtttg
      901 cttatctgca taatgcaaat gccgttattg atgacagact gatttttgat gatgcagcca
      961 ccaccattaa aaatgcattg caggaagtga ttgctaccca taaagataaa aaaggtattg
     1021 cctctgctgc tttcgaaata gctcttaaaa aactgttgta atgcttatag atgttctgca
     1081 tcaaagaata ttaggaaaca ccatagaaga ctacctatgg tttgcaggac ttattctggc
     1141 aggacttgtc ttcaagaatc ttatttcaag aattcttact tttttactct tcaatgttct
     1201 gaaaaaatat ttcaaaacca ttggtgtaaa taagtttgtt gagctgacac agaaaccatt
     1261 ttcagcaaca ctttttctaa tcatcatata cattgcatgc aggtatgtgc attttcctga
     1321 tgaatggaaa ctagcttcag aaagtgagtt tggtgtaaga atgacaataa aattaatttt
     1381 tcagattgtc actgtatttt ttatcacctg gacaatactc agagtgatag attacattgg
     1441 attggtaatg cgcgagcgtg ccaacaaaac cgaatcaaaa atggacgatt tatttgttcc
     1501 gttttttgca tcatcaatga aagcaataac tgtaatcatt agtttatttt ttattcttgg
     1561 tgcagttttc aaactcaatg ttgccagcat cattgccggt ttaggaattg gaggattagc
     1621 catagcttta gcttctaaag acacattaga aaacctattg gggtcatttt tgatttttat
     1681 ggacaaacct tttgtggttg gtgatttggt aaagactgac agtcttcgtg gccatattga
     1741 agaagttggt ttccgttcca ctaaagtacg cactattgaa aaaactttag taattgttcc
     1801 gaataaaaaa atgatggatg cagaattaga aaatcaaacc gaaagaacat tttaccggca
     1861 tcgttttgta ataggcttgg tatattctac aacagctgat cagattcaga atattattca
     1921 gcaaatattg aatatgctca atgaacaccc tttagttaac gaaaatccaa atgtattctt
     1981 taccaacttt ggggcaagtg cattagaaat tgaagtcatt tattttgtga aaacacaaga
     2041 gttgagcgac tttgaaaaca ctaggcagga agtcaatttt ggggtaatga aaatagtagc
     2101 tgaaaataaa agtgattttg cttttccaag tacttcagtt tatattgaaa aggaagtgaa
     2161 aaagtttttg caatgaaaca gtgaatgatg ttaaaaaaga gaagcggttc taaaagaacc
     2221 gcttctcttt tttaaaagtc taaaaattta ctacttcaaa ttttttccaa taaatgcagc
     2281 taaatctttt cctgctttag cataagactc agatatacgt gtgccggtat cataatcata
     2341 gccaccgaaa gtacgaccgg gaaatctcaa accttgcatt ttacaaattg gtttatcagg
     2401 attggctgtt tcatacacag taatttcaag attaatggat gcattttttc tggtaacata
     2461 aacattaaaa ccgggctctg taaaagttgt gctaaccttc attgtatatt ttgcatcagg
     2521 aaaatcacct attaacattg gagatttttt attgaatagc tctacaaaac ttggttcaaa
     2581 tctgttttta cgatctgcca cccagctttt tgcccaacta tctccacgtc cgggttcttt
     2641 tgcattatac tctctggttt ttttagcaac gtactcttct tcctttgcaa aagagcctac
     2701 gcccatatta ctataatcat agacaacatt cagtttagtt tctcctttta atcctgctaa
     2761 actaccgtca aggattttaa ttttctgtgc agaagctgta cttacaacgg ctgcaataaa
     2821 aagtaacgat gcaatttgtt ttttcataat ttattatttt aaacaaaggt aaaacgttct
     2881 tgatgaaatt tgaatgttaa atattcttaa tttaagcatc agtcttctta ttcattcatc
     2941 tgtgttcata ttgctcagtt tattttactc tttcaaatta agtcaattaa acctcatgta
     3001 agtttctcta atgaatttac tatattaccg taaatgcgtt gttctatatt ttcgtttgac
     3061 ggttttacaa aagttttccc tgtaatattt tcaaacaatt caatatatcg atccgatacc
     3121 atctgtacaa tttcatcaga catttctgga actgtttgtc ccgctaatcc ctgaaaatta
     3181 ttttcaataa gccattgtct caaaaattct tttgacaatt gcttttgagg ttgctttctt
     3241 ttttgccttt ctgcataacc atctgcataa aaatatcgtg acgaatcggg cgtgtgaatt
     3301 tcatcaataa gataaacctt gccttgatga acaccaaact catatttggt atctactaaa
     3361 atcaacccac gctgtgcagc catctccgac cctcttctaa acaactgttt tgtataggat
     3421 tctgcctgtt cataaattgc agcatccaca attccctgtt taattatttc ttccttgctt
     3481 atatcctgat cgtgaccatg cgcagcttta gtagtaggcg taattatcgg ctcaggcaag
     3541 gcatcacttt cattcaaacc atcgggtaat ttaacgccac ataacattct ttttccctgt
     3601 ttgtattcgc gccaggcatg accggaaaga tatcctctga taaccatttc tactcttaaa
     3661 ggctgacatg gaatacctat agacacctga acatcgggtg tatcaacaat ccaattgggc
     3721 actaaatcgg cagttgcctg cataaaatgt gctgcaatct gatttaaaac tgctccttta
     3781 tatggaattg gccttggcaa aaccacatca aatgccgaaa cacggtctgt aacgaccatc
     3841 aca
//
LOCUS       DAORQT010000152         3823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4393_length_3823_cov_5.181263, whole genome shotgun sequence.
ACCESSION   DAORQT010000152 DAORQT010000000
VERSION     DAORQT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4393_length_3823_cov_5.181263"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2474)
                     /locus_tag="PK134_05975"
     CDS             complement(<1..2474)
                     /locus_tag="PK134_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB38431.1"
                     /translation="MWSVLSFELSYRFRRPATYIYLAILFLISFLFTATDAISISGAV
                     GNIHKNSPYTIYQVMLILTLLGTMIISAVMGVPVYRDYEHRFHEIMFTLPVTKAQYLG
                     GRFLGSYFTALFIFLGIPLGILTGLLMPWIDKEVIGPFMLNAYVLPFLYIIIPNVFIL
                     GVMFFATGSLFRNQLAIYLQGMVFFILYLVLTSATGDVENNPKFALLDVFGITPSFYI
                     TRYWTPFEKNLQTIPLESYLLWNRLLWVGIALIGAFVTYRLFQFSKSSPFVLRSRKNQ
                     EKISVENLSHINLPETSKDYSFSTKLRQWLQMVRFDFFGTIRSVPFIAIWICGVILIL
                     GQMPQIGKMFGTTIYPVTYAIIDDLTGNFTLFTLIIITFYSGELVWKERERGIHLITD
                     SLPVSRYAMITAKFFAMCLIVAMLQLTVMFMGIFIQTLKGFYRYDIDVYLKTLFVSQY
                     PSLILLVLLAFFIHSMVNNKFIGHTLMIAYFVGRMSAAAMGITHKMIWYGNIPSAPYS
                     DMNGYGHFLYPKYMFLIYWGSLGLVLFIIGVMMNRHGSETGIKARWQIFKQQWKFSRG
                     RKIVWSLLLVFIVSGCFVFYNTNILHKFKSDKTVRHEQAEYEKRFRKYLDFNQPRITA
                     VNVNIDLVPEKRYAAIKGYYIIQNKSALPIDTFLITWAHENIKINRFGFDILADEVEN
                     YQKQFVVYKLAHALKPGDSTKVYFDIEYINKGFANSEEELGLVNNGTFINNNYLPAFG
                     YSESFELTDNSDRKKEDLPRKNHTMRLLSDSASRNNTYLANDADWIRYESIISTSPDQ
                     IAVSPGYLQKEWTQNGRRYFHYKMDAP"
     assembly_gap    2601..2610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2611..3331)
                     /locus_tag="PK134_05980"
     CDS             complement(<2611..3331)
                     /locus_tag="PK134_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB38432.1"
                     /translation="MKLTIKGLTKTYPNGVQALKNINLEIKNGLFGLLGPNGAGKSSL
                     MRTIATLQNADSGNIFLDDLNVLEDKEEVKKILGYLPQEFGVYPNISAQELLTHLALL
                     KGVHIKKERDEVVKSLLMQTNLYEVRNKNLGGFSGGMKQRFGIAQALIGNPKIIIADE
                     PTAGLDPTERNRFLNLLSEISEQVIVILSTHIVEDVKELCSQMAIINKGEVMFSGHPA
                     EAIKQVQGKIWMCQVDKPTARE"
     gene            complement(3405..3758)
                     /locus_tag="PK134_05985"
     CDS             complement(3405..3758)
                     /locus_tag="PK134_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38433.1"
                     /translation="MKRFKILTIILVAAILPALAQNTQPPVVVPALISQGEVIPYQQL
                     PEVIITPNAAEQKAVELPVVTITANKINEQIYPAVKYGNNYIASVTIAPINVVAHQQK
                     RTFASLFSFLRYFSR"
BASE COUNT         1218 a          717 c          702 g         1176 t
ORIGIN      
        1 ggtgcatcca ttttataatg gaaataacgt cttccgtttt gtgtccattc tttttgaagg
       61 tatcccggtg aaacggcaat ctgatcgggt gatgtgctga tgatgctctc atatcttatc
      121 cagtctgcat cattagctaa ataagtgttg ttgcgcgatg cactatcact gagcaaacgc
      181 atggtgtgat tttttcttgg caaatcttct ttctttctgt cgctgttgtc ggtcaactca
      241 aacgattctg aatatccaaa tgcaggcaaa tagttgttat tgataaaagt accgttgttt
      301 accaacccta actcctcttc tgaattggca aagcctttgt tgatgtattc aatgtcgaaa
      361 taaacttttg tagagtcacc gggctttaat gcatgagcta atttataaac gacaaactgt
      421 ttctgataat tttcaacttc atcagccaga atatcaaaac caaacctgtt gatttttata
      481 ttctcatgtg cccaggttat taaaaatgta tcaataggca gtgcagattt gttttgtatg
      541 atataataac ctttaatggc agcgtatctc ttttcaggaa ccaaatcaat atttacattc
      601 actgctgtta tgcgcggctg attaaaatca agatattttc taaatcgttt ttcgtattcg
      661 gcctgctcat gtctgacagt tttatcgctt ttaaatttat gaaggatgtt ggtattgtaa
      721 aagacaaaac atcccgaaac aatgaaaact aaaagtaatg accatacaat ttttcttcct
      781 ctgctgaatt tccattgttg tttaaaaatt tgccaccgcg ctttaattcc tgtttcacta
      841 ccatgacgat tcatcattac accaataatg aaaagtacaa gtcctaagct tccccaataa
      901 attaagaaca tatatttagg ataaagaaaa tgtccatatc cattcatgtc tgagtagggt
      961 gcagaaggaa tgttaccata ccaaatcatt ttatgcgtta tccccatagc ggccgctgac
     1021 attcttccta caaaataggc aatcatcaag gtgtggccga taaatttatt gttgaccatg
     1081 ctgtggatga aaaatgcaag caacacaagt aaaatcaaac ttgggtactg actgacaaaa
     1141 agtgttttta aataaacatc aatatcatag cgataaaatc cttttaaggt ctgaatgaaa
     1201 attcccataa acatgacagt caattgcaac atggccacaa taagacacat ggcaaaaaac
     1261 ttggctgtta tcattgcata tcttgaaaca ggaagtgaat ctgttatcag atgaatgccg
     1321 cgttctcgtt ctttccacac caactcaccg gaataaaaag taataattat cagagtaaac
     1381 aaggtgaaat ttccggtcaa atcatcaatg atagcatagg taacaggata tatcgtagtg
     1441 ccaaacattt tgcctatttg aggcatttga ccaagtataa gaatcactcc acaaatccag
     1501 attgcaataa aaggaacaga gcgtatggtg ccaaaaaaat cgaatcgcac catttgaagc
     1561 cattgccgta attttgttga aaaggagtaa tcttttgaag tttctggaag gttgatgtga
     1621 cttagatttt caacactaat tttttcctga ttttttctgc tgcgcaaaac aaacgggctg
     1681 ctctttgaaa actgaaacaa ccgataagta acaaaagcgc ctattaaagc aatgcccacc
     1741 cacaacaaac gattccacaa cagataactt tccaatggta ttgtttgcag atttttttca
     1801 aatggtgtcc agtagcgtgt aatataaaat gatggtgtga tgccgaatac atcgagtaag
     1861 gcaaattttg gattattttc tacatctccg gttgcagagg taagcactaa ataaagaata
     1921 aagaaaacca tcccttgcag ataaattgcc aattgatttc tgaaaagtga gcctgtagca
     1981 aaaaacatta cacccagaat aaacacattg ggtatgatga tgtacagaaa gggaagtaca
     2041 taggcattca gcataaacgg acctataact tctttgtcaa tccatggcat taataatcct
     2101 gttaaaatgc ctaatggtat tcccagaaaa ataaataagg cagtgaaata tgaacctaaa
     2161 aaacgccctc caagatattg tgcttttgta acgggaagcg taaacataat ttcatgaaag
     2221 cgatgttcat agtcgcgata cacaggaaca cccatcaccg cagagataat catcgtacct
     2281 aaaagagtaa gaatcaacat aacctgataa atggtgtatg gtgagttttt atgaatgttg
     2341 ccaacagcac cactgataga aattgcatct gtagcagtga ataaaaagct gatcagaaat
     2401 aaaatagcta aatagatgta agttgcaggc cttctgaaac ggtacgacaa ctcaaaactg
     2461 agtacactcc acatagctta aggttttaaa ttaccgttaa gaacagaaaa ataaacatct
     2521 tccaaaacgg gctgcgcaat ggcgaagtga tgattaagcg gttcttcgct ccagacatgc
     2581 agttcaatga ggccggtatt nnnnnnnnnn gctcacgagc tgtaggcttg tcaacctggc
     2641 acatccagat tttcccctga acttgtttta ttgcttctgc agggtgacca ctgaacatta
     2701 cttcaccttt attgataata gccatttgag agcataactc tttaacgtct tcaacaatgt
     2761 gtgtagaaag gattacaatt acctgctcgc tgatttcgct aagcaaattc agaaaacggt
     2821 ttctttctgt tgggtcaaga cctgccgttg gctcatcagc aataataatt tttggattgc
     2881 cgattaatgc ctgagcaata ccaaaacgct gtttcattcc accgctaaaa ccacctaaat
     2941 ttttgttgcg cacctcataa aggtttgttt gcattaacaa acttttcaca acttcatcgc
     3001 gctctttttt aatatgaaca cctttaagta aggcaagatg cgtgagcagc tcttgtgctg
     3061 atatgttagg ataaacacca aattcctgtg gcagatagcc caatattttt tttacttctt
     3121 ctttatcttc aagtacattt aggtcatcta aaaatatatt tccgctatcg gcattttgca
     3181 gcgtagctat tgttcgcatc agggatgatt tacctgcacc gttgggacct aacaaaccaa
     3241 aaaggccgtt ctttatttca aggtttatat tttttaatgc ctgcactccg ttgggatatg
     3301 tttttgtcag gcctttaatg gtaagcttca ttggcagaaa tttgattttt atgtttgtaa
     3361 aaaaatgaat gctataaaag cagcaagcaa aataagaggt tttgttaacg gctaaagtag
     3421 cgaagaaatg agaacagtga tgcaaaggtt cttttctgct gatgtgccac cacattaata
     3481 ggtgctattg ttactgaggc aatgtagttg ttgccatact ttactgcagg ataaatttgt
     3541 tcgttaattt tatttgcagt aatcgtaaca acgggtaatt caacagcttt ttgttcagca
     3601 gcattcgggg taatgatgac ttccggaagt tgttgatagg gtatcacctc gccctgactg
     3661 atcaatgcag gaactactac cggtggttgt gtgttttgtg ctaatgcagg caatattgct
     3721 gcaactaaaa tgatggttaa aattttgaag cgtttcatct gtatatgttt ttattgatta
     3781 ttgcgatgca aagatatatt tataaaacag tactatgcaa cac
//
LOCUS       DAORQT010000153         3817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4400_length_3817_cov_8.502658, whole genome shotgun sequence.
ACCESSION   DAORQT010000153 DAORQT010000000
VERSION     DAORQT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_3817_cov_8.502658"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PK134_05990"
     CDS             complement(<1..1236)
                     /locus_tag="PK134_05990"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRB38434.1"
                     /translation="MRKFFYLVLLGSIFSTTVFSQGIVSGVVKDKNHKPVAYANVYVK
                     PGFVGTTTSSDGIFEIELVPGKYTLYCQNLGYKQFSKQIEVGAGKQQIEIELTDELYS
                     LKEVAVTNNKEDPAYEYIRKTIAKRKSYLNEVDAYSCNVYIKGVQKLNKYPEKFLGKV
                     VDFGGVVDSTTGIFYLSESVSKYSFEKPNKIREEMISSRISGNNRAFSFNKASDMQFN
                     FYENRVMSEGISSKGFVSPVAGDALFYYRYKLIGSVVENDRLIHKIEVIPRRQNDPVF
                     RGVIYIADDLWRFQFVDLFLTRDAGIEFLDTLRIRQSFVPVENNVWMPVTNLFDFSFR
                     ILGFQGGGNYIGYFSDFKINPGFPKKTFSAEILKISPDANKKDSAYWDTVRPIPLTID
                     ETSDYFKKDSLAIIRESKQY"
     gene            complement(1292..2218)
                     /locus_tag="PK134_05995"
     CDS             complement(1292..2218)
                     /locus_tag="PK134_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRB38435.1"
                     /translation="MVSISVVVPTYNRLNFVVKTIESLLNQQSDDFEILIVDDGSTDK
                     TEEYFSTYKHPKVTYHRIVNSERGFARNYGASIAKGRFINFFDSDDLAYNNHISTALN
                     YIAKFDNPEIFHLNYVYQTLDGQDLDVLKADSDNIGEMLIKKGNILSCNSVVIRKDIA
                     EKFPFCESRLLSGTEDYALWLALSSRFKIHYVPEITSVVIDHEQRSMASHDIIKMINR
                     NMFLLDHLQADQHFMQYVGKRFNQIQCECFSFIALHLMLEGKNKDGIYFLSKALIADW
                     RFPFRRIRFMAIMKHMLKSFFRIQRSKVPEAV"
     gene            2351..2920
                     /locus_tag="PK134_06000"
     CDS             2351..2920
                     /locus_tag="PK134_06000"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRB38436.1"
                     /translation="MNHRPTEFTERKKGAIEVICGSMFSGKTEELIRRLKRARIAKLK
                     VEIFKPSVDQRYHESNIVSHDSNYIPSSPAQSSSQILLLTNDVDVVGIDEAQFFDLEL
                     PNVCLKLAHQGIRVIIAGLDMDFKGEPFGPIPRLMAIADFVTKVHAICMDCGDLALFS
                     YRTVPDESLIVLGEKESYIPLCRTCYHKR"
     gene            2980..>3817
                     /locus_tag="PK134_06005"
     CDS             2980..>3817
                     /locus_tag="PK134_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRB38437.1"
                     /translation="MYTITEAASILKGTLVLNVNDNQTFRYLLTDSRQSGVAGESLFF
                     AIKGDRHDGHLFINDLIRKGVKNFVVSDTHTSQNKEANFIVVENTLDALQQLASFHRN
                     KFNLKTIGITGSNGKTIIKEWLYQLLQPDFSIVRSPKSFNSQTGVPLSVWQINNEHNL
                     GIFEAGISIPGEMQKLEKIIQPEIGIFSNVGEAHSENFSSLDVKIQEKLLLFQHCKTL
                     IYCKDYSGIHNAVIQSGYLENGLKCFSWSRRIKSDLQIGRIEKQHGGKLKFESEQGVG
                     TVF"
BASE COUNT         1290 a          721 c          624 g         1182 t
ORIGIN      
        1 atactgttta ctctctcgaa tgatagccaa actgtctttc ttaaaataat ctgaagtttc
       61 atctatcgta agtggtatag gtcttacagt atcccaatat gcagaatcct tcttattggc
      121 atcaggactg atttttaaaa tttctgctga aaaggttttt tttggaaaac cgggatttat
      181 tttaaagtca gaaaagtagc ctatataatt tccgcctccc tggaaaccaa gaatcctgaa
      241 actgaaatca aataaatttg tcacaggcat ccaaacatta ttttctacag ggacaaaaga
      301 ctgccttatt ctgagtgtat ctaaaaactc aatacctgca tcgcgtgtaa ggaaaagatc
      361 aacaaactga aatcgccata aatcatctgc aatataaata acacctctga acaccggatc
      421 gttctgtctt ctggggatta cctcaatttt atgaataagt ctgtcgttct ctacaactga
      481 accaattaat ttataacgat aataaaacaa agcatcgccc gcaactggtg agacaaatcc
      541 tttggaagaa atgccttctg acataaccct gttttcataa aaattaaatt gcatatcaga
      601 tgctttatta aagctgaatg ccctattatt accacttatg cgcgaagaaa tcatctcttc
      661 acgaatctta ttcggttttt cgaaactgta ttttgaaacc gattcactta aataaaatat
      721 gcctgttgtt gagtcaacca ctccaccaaa atctacaacc ttaccaagaa atttttcagg
      781 atacttattt aatttctgta cacctttgat ataaacattg cacgaatatg catcaacttc
      841 gttcagataa cttttccttt tagcaattgt ttttctgata tattcatagg ctgggtcttc
      901 tttattgttt gtaacagcaa cctcctttaa cgaataaagc tcatctgtaa gttctatctc
      961 tatttgttgt ttacctgcac caacttcaat ctgtttgctg aattgcttat agcccaggtt
     1021 ctgacaataa agagtgtact taccgggaac taattcaatt tcaaaaatac cgtcactact
     1081 tgttgtcgtc ccaacaaaac cgggtttcac atatacattg gcataagcta caggtttatg
     1141 atttttatcc ttcactactc ctgatacaat accctgagaa aacactgtcg tactaaaaat
     1201 actgcctaac agaaccaggt aaaaaaattt acgcatcaca acaattaatt cttaatttca
     1261 ggatgctaat gtaaaacttt aaatttgggt ttcagacagc ttccggaact ttgcttctct
     1321 gaatgcggaa gaatgactta agcatgtgtt tcataatagc cataaaacga attctacgga
     1381 acggaaaacg ccagtccgct attaaagcct ttgaaagaaa ataaatacca tctttatttt
     1441 ttccttccag catgaggtgc aatgctataa aagaaaaaca ttcacattga atctgattaa
     1501 atcgtttacc aacatattgc ataaaatgtt gatcagcttg aagatgatct aagagaaaca
     1561 tgtttctgtt aatcatcttt ataatatcgt gcgaagccat actacgttgt tcatggtcaa
     1621 ttactacact ggtaatttcc ggcacataat gtattttgaa ccgtgaggac aaggctagcc
     1681 acaaagcata atcttcagtg cctgaaagaa gacggctttc acaaaatgga aacttttcag
     1741 caatatcttt tctgattact acagaattac aacttaaaat attacctttt tttatgagca
     1801 tttcaccgat attatcagaa tctgctttca ggacatctaa atcctgtcca tcaagtgttt
     1861 gataaacgta attaaggtga aaaatttcag ggttatcaaa cttcgcaata taattcagtg
     1921 cagtagaaat atgattatta taagctaaat catctgaatc aaaaaaatta ataaacctac
     1981 ctttagcaat tgatgctcca tagttacgtg caaatccacg ctcgctgttt acaattctat
     2041 ggtaggttac ctttggatgt ttataggttg aaaaatattc ctctgtctta tcggtactac
     2101 catcatcaac aatcaaaatt tcaaaatcat ctgactgttg attcagcaat gactcaatcg
     2161 tttttacaac aaaattcaac ctattataag ttgggactac tacagaaatg gaaaccatga
     2221 gaaaaagtat tgcaaaacta cagtacaata actgaaaaca aacatttctt aaaaaaaaat
     2281 tatccgctaa atgttaaccg gctaaaaatt gcagcaaaca gattttacct atttttgaaa
     2341 ccgttaagat atgaaccata gaccaacaga atttacagag cgaaaaaaag gcgctattga
     2401 agtcatttgc ggcagcatgt ttagtggtaa gacagaggag ttgattcgca gactaaaacg
     2461 agcgcgcatt gccaagctta aggttgaaat atttaaacct tctgttgatc aacgctatca
     2521 tgaaagcaac attgtttcgc acgattcaaa ctatattcca tcatcccctg cccaatcatc
     2581 ctctcaaatt cttttactca caaacgatgt tgatgttgta ggcattgacg aagcgcaatt
     2641 ttttgatttg gaactgccta acgtttgcct taaactggca catcagggaa tcagggtaat
     2701 tattgcaggt ttagatatgg actttaaagg cgaacctttc ggacctattc caagacttat
     2761 ggcaatagca gactttgtta caaaggttca tgccatctgt atggattgtg gcgaccttgc
     2821 cttattttct tatcgtaccg tacccgatga atcactcatt gttctgggtg aaaaagaaag
     2881 ctacattcct ttgtgcagaa cttgttatca taaaaggtaa ttgttaccaa tttgatgcac
     2941 tatcaaagct taattttgta ggctattcaa tttaaaaata tgtacaccat tacagaggca
     3001 gcgtcaatat taaaaggaac gctggttttg aatgtaaacg ataatcagac tttccgttat
     3061 ctgctaacag acagccgcca aagcggtgtg gccggtgagt cacttttttt tgcaattaaa
     3121 ggcgaccgcc atgatggtca tttatttatt aatgatctta tcagaaaagg agtaaaaaac
     3181 tttgttgtta gcgatacaca cacttcacaa aacaaagaag ctaattttat tgtggtggaa
     3241 aacacacttg acgcactaca acaacttgcc tcgtttcatc ggaataaatt taatctgaaa
     3301 acaataggaa taactggaag caatggaaaa acaattatca aggaatggct ttatcaatta
     3361 ttgcaaccag attttagtat tgtccgcagc ccaaaaagtt ttaattctca aacgggcgta
     3421 ccattatctg tttggcaaat aaacaatgag cataacctcg gaatttttga agcaggcatt
     3481 tctatacctg gtgaaatgca aaagttggaa aaaattattc agcctgaaat tggaattttc
     3541 agtaatgtcg gtgaagcaca cagcgaaaac ttttcttcac ttgatgttaa aattcaggaa
     3601 aaattattgt tatttcaaca ttgcaaaacc ctgatatact gcaaagacta ttcaggaatt
     3661 cacaatgctg taatacaatc tggatatctt gaaaacggac taaaatgttt tagctggtcg
     3721 cgaagaataa aatccgattt acagattggc agaatagaaa agcaacatgg gggtaagtta
     3781 aaatttgagt cagaacaagg tgttggaacg gttttta
//
LOCUS       DAORQT010000154         3810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4415_length_3810_cov_5.845806, whole genome shotgun sequence.
ACCESSION   DAORQT010000154 DAORQT010000000
VERSION     DAORQT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_3810_cov_5.845806"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1209)
                     /locus_tag="PK134_06010"
     CDS             complement(<1..>1209)
                     /locus_tag="PK134_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRB38438.1"
                     /translation="LGKRILGEDFSVNREIKTATTASVQTSLFDDEIVRSESDVVRGK
                     NIQNTPHNYTLVENNEAIDGLVAVLLKQKEICFDTETSSLDYFSLSIVGLSFSFNSGT
                     GYYIPCPEDFEKAKNICHKFKPILEHVSIIKIGQNIKFDMLVLKRYGVEVRAPLYDTM
                     IAHYLVEPDMKHGMDYLSETYLGYTPVHIEELIGKKGKNQGSMRDVPIDKIAEYASED
                     ADITFQLKQVLAPMVEKHEVDGVLKDIELPLIPVLADMEYEGVKIDVDYLNAYSKELE
                     QDMLKYRSAIFEIAGLEFNLDSPRQLGDVLFGHLKLPVDKKTATGQHSTNEEVLSKLA
                     LEYPIAEKILEYRELTKLKSTYVDALPQMVNVKTGRVHTTFNQTIASTGRLSSIGPNL
                     QNIPIRTERG"
     assembly_gap    1210..1219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1220..1468)
                     /locus_tag="PK134_06015"
     CDS             complement(<1220..1468)
                     /locus_tag="PK134_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRB38439.1"
                     /translation="MGEKTAKKLIKEYGSMENVIANAENIKGKLGENIRSNVEQARIS
                     KQLATIILDVPVEVNDDDLLMSLPDKEKLSQLFTELEFR"
     assembly_gap    1492..1501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1502..2058)
                     /locus_tag="PK134_06020"
     CDS             complement(<1502..2058)
                     /locus_tag="PK134_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRB38440.1"
                     /translation="MNEDKRLFLLDAYALIYRAYFAFSKTPLVNSKGQNVSAVSGFTS
                     TLFDLMRREKPTHIAVVFDAAAETTRAAEHSFYKANRQEMPEDIQTAVPYIKEIIKAF
                     HIPILEYDGYEADDIIGTVAKQKEEEGYTVFMVTPDKDYAQLVSPNIFIYKPGRLGND
                     IEILGVKEIQEKWEVENPKQVIDILG"
     gene            complement(2055..3194)
                     /locus_tag="PK134_06025"
     CDS             complement(2055..3194)
                     /locus_tag="PK134_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRB38441.1"
                     /translation="MEAIYLDYNATTPVDERVLEKMLPCFSKKFGNAASNTHAFGWTA
                     KEAVEIAREKTSALIGCEPRELYFTSGATEGVNLIIKGVYESYSKKGKHIITYATEHK
                     AVLDTCKYLQTIGADVEILPVERDGLPDLDLFKKSLRADTILAIAMFANNETGVLFPI
                     AEMGDICRGHDVIFFSDTTQAAGKVLFDVKESNIDCCVLSAHKMYGPKGVGAVYISRK
                     NPRVSVQAQLHGGGHEGGMRSGTLNVPGIVGLGEACHIAEKEMWDNGILLSSLRTRLE
                     QQLESDGSVMINGSIKNRLNNTSNLTFKAIKSEDLIKEIRPVAVAAGSACTAALPQPS
                     HVLLAMGLTEKEAYNSVRFSLGKYTTAHEIDLTIEKIRKIISSKI"
     gene            complement(3283..3621)
                     /locus_tag="PK134_06030"
     CDS             complement(3283..3621)
                     /locus_tag="PK134_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38442.1"
                     /translation="MRKFRILYSILPLLCFVSFSFAGGQAEIKQHASPIQLKSENHKS
                     DVHCDIHHVKVSFIVAEDGLVKVSSIGTRNTMLREYIMKKLAKLNFKGMNVEGNYSVT
                     LSFDLGDKCT"
BASE COUNT         1160 a          797 c          572 g         1261 t
ORIGIN      
        1 gtccacgctc tgtcctgatg ggtatgttct gaagattggg cccaatagaa ctcaaccttc
       61 ctgttgaagc aatagtttga ttgaaagtag tatgtaccct gccggttttg acattaacca
      121 tctgaggcaa tgcatcaaca taagttgatt ttaatttagt aagttcacgg tattcaagaa
      181 tcttctcggc aattggatat tcaagtgcca atttgctcaa cacttcttca ttggttgaat
      241 gttgccccgt agcagttttc ttgtcaacag gtaatttcaa atgtccaaat aatacatctc
      301 ctaactgacg tggtgaatct aaattaaatt ccaaacctgc aatttcaaaa atagctgaac
      361 gatacttcag catatcttgt tccaattctt tagaataagc gttcaaataa tccacatcta
      421 ttttaacacc ttcatattcc atatcggcca atacaggaat cagtggcagt tcaatatctt
      481 ttaaaacacc gtcaacctca tgtttttcaa ccataggtgc caacacttgt ttcaactgaa
      541 atgttatatc tgcatcttct gatgcatatt cagcaatttt atcaataggg acatcacgca
      601 tgctcccctg attctttccc ttcttgccaa taagttcttc aatatgcaca ggtgtgtagc
      661 caagataagt ctcactcaga taatccatgc catgtttcat atcaggctca actaaataat
      721 gtgcaatcat ggtgtcatac aaaggtgctc tcacttcaac accatatcgt ttcagcacaa
      781 gcatatcaaa ctttatattt tgtcctattt taataattga aacatgttca agtataggtt
      841 taaatttatg acaaatgttt tttgctttct caaagtcttc tggacaagga atgtaatagc
      901 ctgttccact gttgaacgag aatgataaac ctacaatact taatgaaaaa taatctaaag
      961 aagatgtttc tgtatcaaaa caaatttctt tttgttttaa taatactgca accaaaccat
     1021 caatagcttc attattctct accaacgtat aattatgtgg tgtgttctga atattctttc
     1081 ctctgacaac atcactttca cttcttacaa tctcatcatc aaataaacta gtttgcacag
     1141 aagccgttgt ggctgttttt atctcacgat ttactgaaaa atcttctcca agaattcttt
     1201 tacccaaagn nnnnnnnnnc ctgaattcaa gctctgtaaa aagctgagat aatttttctt
     1261 tgtcaggcaa agacattaat aaatcatcat cattgacttc aacaggaaca tcaagaatta
     1321 ttgtggctaa ttgttttgaa attcttgcct gttcaacatt actgcggata ttttcaccca
     1381 acttaccttt aatgttttct gcattggcaa tcacattctc catactgcca tattctttaa
     1441 ttaacttttt tgcagttttc tcacccacac tgggtatgcc cggtatattg tnnnnnnnnn
     1501 ncccaaaata tcaattacct gttttggatt ttccacttcc cacttctcct gaatttcttt
     1561 cactcccaaa atttcaatgt cgttacctaa acgtccgggc ttataaataa aaatattggg
     1621 cgacactaac tgcgcatagt ccttatccgg tgttaccata aaaacagtat aaccttcttc
     1681 ctctttttgt ttggccacag ttcctattat gtcatcagcc tcataaccat catattccaa
     1741 aataggaata tgaaatgctt tgataatttc tttgatatac ggaactgctg tctgtatatc
     1801 ctcaggcatt tcctgtctat ttgccttgta aaaactatgt tcggctgcac gagttgtttc
     1861 tgccgcggca tcaaacacta cggcaatatg tgttggcttt tctcttctca ttaaatcaaa
     1921 aagcgttgat gtaaatccac taacagcaga cacattctgt cccttgctgt ttacaagcgg
     1981 ggttttgcta aatgcaaaat atgccctata aattaatgca taggcatcga gtaagaaaag
     2041 acgtttgtct tcattcatat ttttgaagaa ataatttttc tgattttttc aattgttaaa
     2101 tcaatttcat gtgcagtagt atattttccg aggctgaaac gcacagaatt ataagcctct
     2161 ttttctgtca accccattgc aagcaataca tgtgacggtt gtggcaatgc cgcagtgcaa
     2221 gcagaacctg ctgcaacggc aacaggtctg atttctttta taaggtcctc gctctttatt
     2281 gccttgaatg taagattgga tgtattattc aaacgattct ttatacttcc atttatcatc
     2341 acacttccat ctgactctaa ttgttgctca agtcttgtgc gaagtgacga taataaaatt
     2401 ccattgtccc acatttcttt ctctgcaata tgacatgctt caccaagccc tacaattccc
     2461 gggacattta atgttccgga gcgcatacca ccttcatgac caccaccatg caattgtgct
     2521 tgaacagaaa ctcttggatt tttccgactg atataaactg cacctacacc ctttggccca
     2581 tacattttat gagcggacaa aacacaacaa tctatattac tttctttgac atcaaataaa
     2641 actttacctg cagcctgagt agtgtcagaa aagaaaatca catcatggcc tctgcatata
     2701 tcacccatct ctgcgattgg gaacaaaact cctgtttcgt tattggcaaa catggcaatt
     2761 gctaaaatgg tatctgcacg caaagacttt ttaaataaat caagatcagg taagccatct
     2821 cgttcaacag gcaaaatttc tacatctgcg ccaattgttt gcagatactt acaggtatca
     2881 agaacagctt tatgttctgt tgcataggtg atgatgtgtt tccccttttt tgaataactt
     2941 tcataaacac ctttaataat cagatttact ccttctgtgg cgcctgatgt aaaatataat
     3001 tctcgaggct cgcatccgat aagagcactc gttttttcgc gggcaatctc aactgcttct
     3061 tttgctgtcc atccaaaagc atgtgtattg cttgcagcat taccaaattt ttttgaaaaa
     3121 caaggcaaca tcttttccag cacacgttca tctacaggtg tggtagcatt atagtctaaa
     3181 taaatggctt ccatctcaag gtcaaagaaa gaaaatattt tgcctatatc agatggaaat
     3241 ataagcttaa ctaaaaatgt atctctaaaa ctacagcatt aatcaggtac acttatctcc
     3301 caggtcaaaa cttagtgtga cactgtagtt accttcaacg ttcattccct taaaattcaa
     3361 cttagcaagt ttcttcataa tgtactctcg caacatggtg ttgcgtgtgc caatgcttga
     3421 aacttttacc aagccatctt ctgcaacaat aaatgaaact ttaacatgat gaatatcgca
     3481 atgcacatca gacttatgat tctcagactt taactgtatg ggtgaagcat gttgtttaat
     3541 ttcagcctga ccacctgcaa acgaaaaact aacaaaacaa agtagtggaa gtattgaata
     3601 aagtatgcgg aactttctca tgattataac ttttaagttt aatatatgac gcccataacg
     3661 aacaaaaagt tacccaaatg tagtatattt tccaattgtt taaagaaatt tctaaaaatt
     3721 tttaaaagta tcagtaagtt tgacccataa gtgaataatt aattataaag ttaaatatta
     3781 tgcgtctgaa attaaatctg ataactttag
//
LOCUS       DAORQT010000155         3806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4422_length_3806_cov_7.637963, whole genome shotgun sequence.
ACCESSION   DAORQT010000155 DAORQT010000000
VERSION     DAORQT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4422_length_3806_cov_7.637963"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK134_06035"
     CDS             complement(<1..1197)
                     /locus_tag="PK134_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HRB38443.1"
                     /translation="MKHQISSKHLDFNTLKLIIDNKATLELSEDAVKRITHCRSYLDN
                     KVSSLAEPVYGINTGFGSLYNKSIDTADLGKLQENLVMSHACGCGEEVPAAIVKLMLF
                     LKVQGLSYGNSGVQLQTVQRLIDFYNHDIFPVVYTQGSLGASGDLAPLAHLCLPLIGM
                     GEVYFKGVKVKSAEVLKKFGWQPIKLQSKEGLALLNGTQFMSAYGTHAVMQSQRMMLL
                     ADIIGALSLDAYDGRIEPFHHLIHQIRPHKGQISTALRYRQLLADSEMIKRKKAHVQD
                     PYSFRCIPQVHGASKDALDHVSYVIFNEINSVTDNPTVFPDEDLVISAGNFHGQPLAL
                     VLDFLAIALSELANISERRTYQLISGQRGLPSFLVADPGLNSGMMIPQYTAASIVSQN
                     KQLCMPA"
     gene            complement(1232..2596)
                     /gene="hisS"
                     /locus_tag="PK134_06040"
     CDS             complement(1232..2596)
                     /gene="hisS"
                     /locus_tag="PK134_06040"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRB38444.1"
                     /translation="MKPSIPKGTRDFSPSEMVRRNFIFNTIKKVYERYGFVQIETPAM
                     ENLSTLEGKYGEEGDKLLFRILNSGEVVQDSKSKGENLKISDLAEKGLRYDLTVPFAR
                     YVVMHQNEISFPFKRYQMQPVWRADRPQKGRYREFYQCDADVIGSTSLISEAEFLLII
                     QEVFSSLQIDVAIKINNRKILAGIAEVIGHADKIIDITVAIDKLDKIGEQKVKEELLA
                     KGLSPDAVNKMQPLFESRGDFETKLNFLKTFLAESSIGLKGIEETENVISYLNAFGKQ
                     LKNLELDITLARGLNYYTGSIFEVKVNDSRSTFSGSVLGGGRYDDLTGIFGLPNVSGV
                     GISFGIDRIYDVMLEMNLFDQLKLSETTSQVLLANFGREEEQFSLKILNELRQAGINA
                     EIFPEYSAKMKKQMSYADSKKIPFVVLIGKDEMQSGSLTIKDMATGQQEKLTLSKIIS
                     KLSK"
     gene            complement(2618..>3806)
                     /gene="ettA"
                     /locus_tag="PK134_06045"
     CDS             complement(2618..>3806)
                     /gene="ettA"
                     /locus_tag="PK134_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRB38445.1"
                     /translation="KVTKTKGERRRLALCRLLLQQPDVLLLDEPTNHLDAESVDWLEQ
                     HLKQYAGTVIAVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSNRLAQEEKQ
                     ESKRQKTLQRELEWVRMSPKARQSKGKARLNAYENLLSEDVKQKEEKLEIFIPNGPRL
                     GNEVIEAIKVSKAYDNKVLYDDLNFKLPPNGIVGIIGPNGAGKTTIFRMVMGLEKPDS
                     GEFKIGSTVRIGYADQTHKEIDPEKTVWEVISGGNESIELGGKLLNSRAYVARFNFSG
                     ADQGKKCGVLSGGERNRLHLALTLRSEANVLLLDEPTNDIDVNTLRALEEAIENFAGC
                     AVIISHDRWFLDRVCTHILAFEDDSQVYYFEGGYSDYEENKKKRLGDITPHRVRYKSL
                     VKN"
BASE COUNT         1077 a          844 c          662 g         1223 t
ORIGIN      
        1 tgcaggcata cacaattgtt tgttctgact tacaattgat gctgctgtgt attgcggaat
       61 catcattccg gaattcagtc cagggtctgc aacaagaaaa gatggcaaac cacgttgtcc
      121 ggaaataagt tgataggttc tgcgttctga aatatttgcc aactcactta aagcaatagc
      181 taaaaaatcg agaactaatg ctagtggttg tccgtgaaag tttcctgctg aaataactaa
      241 atcttcgtca ggaaaaactg taggattgtc tgtaacagaa tttatttcat taaagattac
      301 gtagcttaca tgatccaatg catctttact tgcgccatga acttgcggaa tacatctgaa
      361 tgaatatgga tcctgcacat gcgccttttt tcttttaatc atttcactgt ctgccaacaa
      421 ctgacgataa cgcaatgctg ttgatatctg tcccttatga ggtcgaatct gatgaatgag
      481 atgatgaaat ggttcaattc tgccatcgta agcatctaat gaaagcgctc cgattatatc
      541 tgcaagcaac atcatacgtt gcgactgcat aactgcatgt gtgccgtagg cactcataaa
      601 ctgtgtacca ttcagcaatg caagtccttc ctttgactgc agtttgattg gctgccatcc
      661 aaacttcttg agaacttctg ccgactttac ttttactcct ttaaaataca cttcgcccat
      721 acctatgagc ggcaaacaaa gatgtgccaa cggtgccaaa tcacctgatg cgcctaatga
      781 cccctgtgta taaacaacag gaaaaatatc atgattataa aaatcaatca aacgttgaac
      841 cgtttgcaac tgcaccccgg agttgccata actaagtccc tgtactttca gaaacagcat
      901 taacttaaca atagcagcag gcacttcctc gccacaacca caggcatgcg acattaccaa
      961 attttcctga agtttaccta aatcagctgt atcaatactc ttattgtata aagaaccaaa
     1021 gcctgtgtta ataccataaa ccggttcagc aagtgagctg actttgttat cgagataact
     1081 gcggcaatgc gtaatccgtt taactgcatc ctcagaaagt tcaagagtag ctttgttatc
     1141 aatgattagt ttgagtgtat taaaatccag atgttttgat gatatctgat gtttcatttt
     1201 tgacagttgt tacttagtat attaatagct attacttgct tagcttagaa attattttgc
     1261 ttaaagttaa cttttcctgt tgccctgttg ccatatcttt tatagtgagg cttccacttt
     1321 gcatttcgtc tttaccgatt aatactacaa aaggaatttt ttttgagtcg gcatagctca
     1381 tttgtttttt catcttggct gaatattccg gaaaaatctc tgcattgata cctgcctgcc
     1441 ttagctcatt caaaatcttc aaactaaact gctcttcttc ccgaccgaag tttgcaagta
     1501 atacctgaga agttgtttca ctcaatttaa gttgatcaaa caaattcatt tcaagcatta
     1561 catcataaat tctgtcaatt ccaaaagata ttcctacacc actgacatta ggcagtccaa
     1621 aaattcctgt aaggtcatca tagcgtccac caccaagcac actgccactg aaagttgaac
     1681 gcgagtcatt aaccttcact tcaaaaattg aacctgtata ataattaagc ccacgtgcaa
     1741 gggttatgtc taattcaaga tttttcagtt gctttccaaa agcattaaga taacttataa
     1801 cattttctgt ctcttcaata ccttttaatc ctattgaaga ttcagccaaa aatgttttca
     1861 ggaagttgag ttttgtttca aaatcaccgc gcgattcaaa aagcggttgc attttattta
     1921 ccgcatcagg cgaaagtcct ttagccaaaa gttcttcctt aactttctgt tctcctatct
     1981 tatcgagttt atcaatggca acggtaatgt caattatttt atcggcatga ccaataactt
     2041 cggcaatgcc tgccagaatt tttctgttat ttatctttat ggcaacatca atctgcaaac
     2101 tgctgaacac ttcctgaatg attagaagaa attctgcttc gctgattaat gaagttgaac
     2161 caataacatc ggcatcgcac tgataaaatt cacgataacg tcctttctgt ggacggtcgg
     2221 cacgccatac cggttgcatc tgataacgtt taaacggaaa gctgatttca ttctgatgca
     2281 tgactacata acgtgcaaac ggaactgtta aatcatatcg caagcctttc tctgccaaat
     2341 cagaaatttt aagattttca ccttttgact ttgaatcctg aacaacttcg cctgaattca
     2401 atattctgaa taaaagttta tcaccttcct caccatactt accttcaagt gttgaaaggt
     2461 tttccattgc aggtgtttca atctgtacaa acccatagcg ctcatatacc tttttgatgg
     2521 tattgaaaat aaagtttctg cgtaccattt ctgatggtga aaaatcgcga gtgcctttgg
     2581 ggatggatgg tttcatttgg tgctgcgaat tacgaatcta gttttttacc aaacttttat
     2641 acctcactct atgaggagtg atatcgccca atcgtttttt cttattctct tcgtagtcgg
     2701 aatatccgcc ttcgaaataa tatacctgcg agtcgtcttc gaaagcaaga atgtgtgtac
     2761 ataccctatc taaaaaccat ctgtcgtggc tgatgataac tgcacagcct gcaaaattct
     2821 cgattgcttc ttctaaggca cgcaatgtgt ttacatcaat gtcgttggta ggctcatcaa
     2881 gtaacaacac gttggcttcg cttcgtaatg tcaatgcaag gtgcaatctg ttgcgctcac
     2941 caccggaaag aacaccgcat ttttttcctt gatcggcacc actgaaatta aatcgtgcaa
     3001 cataagcacg agagttcaga agtttgccgc caagctcaat agattcattg ccaccggaaa
     3061 ttacttccca tactgttttt tcagggtcaa tttctttatg cgtttgatcg gcataaccaa
     3121 tgcgaaccgt actgccgatt ttaaactctc ctgaatcggg cttctcaagt cccattacca
     3181 ttcggaaaat tgttgtcttt ccggcaccgt tagggccaat gattcctaca atgccattag
     3241 gtggtaattt gaaattgaga tcatcataca aaactttatt gtcataagct tttgaaactt
     3301 taatggcttc aattacttca ttgcccaaac gtggtccgtt aggaataaat atttctagct
     3361 tctcttcttt ttgttttaca tcttcgctca acaggttttc gtaagcattc aaacgtgcct
     3421 taccttttga ctgtcttgcc ttgggcgaca tgcgcaccca ctctaactca cgttgcaaag
     3481 ttttctgccg cttactttcc tgcttctctt cctgtgcaag gcggtttgat ttctgctcta
     3541 accagcttga ataatttcct ttccatggaa taccttcgcc acggtcgagt tcaagaatcc
     3601 atcctgcaac gttgtctaag aaataacggt cgtgcgttac ggcaataact gttcctgcat
     3661 attgtttaag atgttgttcc aaccaatcaa cagactctgc gtccaaatgg tttgtaggct
     3721 catcaagcaa caatacatcg ggttgctgca ataacagacg acataaagca agtcttctgc
     3781 gctcgccttt ggtttttgta accttt
//
LOCUS       DAORQT010000156         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4473_length_3776_cov_6.983875, whole genome shotgun sequence.
ACCESSION   DAORQT010000156 DAORQT010000000
VERSION     DAORQT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4473_length_3776_cov_6.983875"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PK134_06050"
     CDS             <1..449
                     /locus_tag="PK134_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38446.1"
                     /translation="IIWYVVYRLRKKKPQTVIKVPDAPKRPAHEIAIEQLEHLQQEKL
                     WQQGNFKMYYTQLSDIMRAYISNRWNFDAMESTTDEIMHSNTALQLSNDNFNRLKSTL
                     TLSDLAKFAKYTPLSNENEQAMTDAFIFVNETKVVLTTKPQEQVKP"
     gene            471..1454
                     /locus_tag="PK134_06055"
     CDS             471..1454
                     /locus_tag="PK134_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRB38447.1"
                     /translation="MQFAYPQFFWLLLLIPAMLLWQWKVTSAKKVRLKLSSDDGFQNY
                     QPSFRQRLVFMPFVFRLLSVICIIIALARPQSSSQAQQVSTEGISIVLAMDISGSMLA
                     EDFKPNRIEAAKKVAIDFIDHRPNDLIGLVIFSGESFTQCPLTSDHAVLKNLMSKIES
                     GMLTDGTAIGEGLATAVNRLKDAKTKSKVIILLTDGVNNAGAIAPLTAGEIAKTFGIR
                     VYTIGVGTVGMAPYPFKTVFGIQYQNVPVQIDEEICKQISDATDGKYFRATNNKTLKE
                     IYSEIDKLEKTKVEVTEFRRHSEEYFNYALAAGIFFALELLLKYTWLRKLT"
     gene            1478..2530
                     /locus_tag="PK134_06060"
     CDS             1478..2530
                     /locus_tag="PK134_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRB38448.1"
                     /translation="MFRFSHSFILYCLALIPLFIILYISLVNWRKKSLKKFGDMHLVR
                     FLTPGKAYTKPLIKFILLMTAFLFLVIGIAGPQVGTKLETVKRKGVDVIIALDVSNSM
                     NAEDIKPSRLERAKQSISRLIDQMQGDRIGLIVFAGEAYTQLPITTDYGAAKLFLSTV
                     NTNIVPTQGTAIGKAIDLAVSSFGNEDSSKKNKAIIVITDGENHEDDAVAAAKEAYKN
                     NITVHTIGMGSVNGAPIPVYTNGQRTGFMQDNNGQTVITKIDEQTLAEIAQAGNGKFI
                     RATNSDDGLSIIMKEINAMQKNEFGSKMFTDYADQFQYLLGAALFFLILEFLISEKRS
                     RWMEELNLFGDGDEKK"
     gene            2517..3239
                     /locus_tag="PK134_06065"
     CDS             2517..3239
                     /locus_tag="PK134_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38449.1"
                     /translation="MKKSNILILFFISMSFVAIAQNEKPLVREGNKEYKNGKFDQAES
                     SYRKAIEKNGNSVEGLYNLGNALYKQNKFDEAAGVYQNLNNEKLSTDAKAKSLYNLGN
                     TLMKSEKYQEGANAYKEALKLSPNDKDALYNYSYALAKLKQQQQQQKQNQDNKKDQKN
                     QQKKQQDKQQQKQDQQKQQQDQQKEQDQQKQDQKKEQAQQPKISKEDAERMLEALKND
                     EMNLQKKLAKKEGTRVNIEKQW"
     gene            3233..>3776
                     /locus_tag="PK134_06070"
     CDS             3233..>3776
                     /locus_tag="PK134_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRB38450.1"
                     /translation="MVETKAIKIKHKSLFAGLVLLLLFFTTNIFAAEITFIASVNKNP
                     VGTNEQFSITYTLNTQGSNFQAPTFRDFNVLSGPNQSTSMQFINGNMSQSVSFTYYLT
                     ANSEGTFRIDPATITVNGGKVKSNTLTINVVKSQKAQSGAQQQKSNDADAGISSNSVF
                     LRVSVNKSSVYRGEALLATYK"
BASE COUNT         1400 a          640 c          713 g         1023 t
ORIGIN      
        1 caataatatg gtatgttgtc tatcgcctga gaaagaaaaa accacagaca gttataaaag
       61 ttcctgatgc tccaaagcga ccagctcatg aaattgcaat agaacaatta gagcatcttc
      121 aacaagaaaa attatggcaa caaggtaatt tcaaaatgta ttacacacaa ctttcagata
      181 taatgagagc atacatcagc aaccgttgga attttgatgc catggaaagt accactgatg
      241 aaataatgca tagcaacact gccttgcaat taagcaatga caattttaac agactgaaaa
      301 gcacattaac gttaagtgat ttggcaaaat ttgcaaaata cacaccgttg agtaatgaaa
      361 atgaacaagc aatgacagat gctttcattt ttgtaaacga aaccaaggtt gttctgacca
      421 caaaaccaca agaacaggtt aagccataaa acatgagttt atttgataac atacaatttg
      481 cctatcctca gttcttctgg ttgctgttgc tgattccggc aatgttgttg tggcagtgga
      541 aagttacttc tgcaaaaaaa gtcaggctta aactttcatc tgatgatgga tttcaaaatt
      601 atcaaccaag tttcagacag cgattggtgt tcatgccatt tgtattccga cttctcagcg
      661 ttatctgcat tatcatagct cttgcacgtc cacaatcatc atcacaagca cagcaggttt
      721 caacagaagg catatcaatt gttttggcaa tggacatatc gggaagtatg cttgcagaag
      781 attttaaacc aaacagaatt gaagctgcta aaaaagtggc tattgatttt attgatcatc
      841 gtccgaatga tttaattggt ttggtaattt tcagtgggga aagttttact caatgtccat
      901 taacttcaga ccatgcagta ttaaaaaact tgatgtcgaa aattgaaagt ggtatgctga
      961 cagatggcac tgctattgga gaaggcttgg caacagctgt gaaccgtctg aaagatgcta
     1021 agacaaaaag taaggttatt attttactta ctgacggtgt aaataatgca ggagccattg
     1081 caccattaac agcaggtgaa atagctaaaa ctttcggcat cagagtctat accattggcg
     1141 ttggcacagt tggcatggca ccctatccgt ttaaaactgt ttttggaata caatatcaga
     1201 atgttcctgt gcagattgat gaggaaatct gcaagcaaat ttctgatgca acagatggaa
     1261 aatatttccg tgcaactaac aataaaactt taaaagaaat ctattctgaa attgataaac
     1321 tcgaaaaaac aaaagttgaa gtaactgagt tcaggcgtca tagtgaagag tatttcaatt
     1381 atgctctggc agcaggaata ttttttgcac tagaactact attgaaatat acctggttaa
     1441 gaaaacttac ataaaagata aagattaaag cgtaacaatg ttccgatttt cacatagttt
     1501 tattttatat tgtctggctc tgataccgct atttatcatt ctatatataa gtttggttaa
     1561 ctggcgaaaa aaatcactga aaaaatttgg agatatgcat ttggtcagat ttctcactcc
     1621 gggaaaagca tataccaagc cacttataaa gtttatttta ctgatgactg cgtttctatt
     1681 tctggtaatt ggaattgctg gtcctcaggt aggcactaaa ttagaaactg ttaaacgcaa
     1741 aggtgttgat gtcatcattg cgttagatgt gagtaacagc atgaatgccg aggacattaa
     1801 accaagccga ctggagcgag cgaaacaatc aatttcaagg ttaattgatc agatgcaagg
     1861 cgacagaatc ggcttaatag tttttgcagg tgaagcttat acacaactac ctataactac
     1921 agactacggt gctgcaaaat tgtttctttc aacagtaaac accaacattg ttccaacgca
     1981 gggaactgcc attggaaaag ctattgactt ggctgtatca tctttcggca atgaagattc
     2041 ttcgaaaaaa aacaaagcca tcattgtgat tactgatgga gaaaatcatg aggatgatgc
     2101 agtggccgca gctaaagaag catataaaaa caacattact gttcatacca ttggcatggg
     2161 ttctgttaat ggtgctccta ttccggttta tacaaatggg caacgcacag gatttatgca
     2221 ggacaataat ggtcagactg taatcactaa aatagatgag caaaccttag cagaaattgc
     2281 acaggccgga aatggcaaat ttatcagggc aacaaatagt gatgatgggc ttagcattat
     2341 aatgaaagaa atcaatgcta tgcaaaaaaa cgaatttggc agtaagatgt ttacagacta
     2401 tgcagaccaa tttcaatatc ttttgggtgc tgcattgttt tttcttatct tagagtttct
     2461 gatttcagaa aaaagaagca gatggatgga agaattaaat ttatttggcg atggtgatga
     2521 aaaaaagtaa tattcttatc ttatttttta tttcaatgag ttttgtagct attgcacaaa
     2581 atgaaaaacc attggtacgc gagggcaata aggaatataa gaacggaaaa tttgatcagg
     2641 cagaatcaag ctatcgtaaa gccatcgaaa aaaatggaaa ttctgtagaa ggtctttata
     2701 atttaggcaa tgcactgtac aaacaaaaca aatttgacga agctgcagga gtttatcaaa
     2761 acctgaacaa tgaaaaactt tctactgatg ccaaagctaa atctctttac aacctcggta
     2821 acacattgat gaaatccgaa aaatatcagg aaggtgcgaa tgcctataaa gaggctttaa
     2881 agcttagtcc aaatgataaa gatgctttat ataattattc ttatgcatta gctaaattaa
     2941 aacaacaaca acagcagcaa aaacaaaatc aggataataa aaaagatcaa aagaatcaac
     3001 aaaaaaagca acaggataag caacaacaaa aacaagatca acaaaagcag caacaagacc
     3061 aacaaaaaga acaggatcaa caaaaacagg atcagaaaaa agagcaagca cagcaaccaa
     3121 aaatttcgaa agaagatgca gaacgtatgc ttgaagcact gaaaaatgat gaaatgaatt
     3181 tgcagaagaa attagctaag aaagaaggca caagagttaa tattgaaaaa caatggtaga
     3241 aacaaaagct ataaagataa aacacaagtc gttattcgca ggcttagtgc ttctattgtt
     3301 gttttttaca actaatattt ttgctgcaga aataactttc atagcatctg taaacaaaaa
     3361 ccctgttggc accaatgagc aattcagtat aacctatact ttaaacacac aaggcagtaa
     3421 tttccaagcc ccaactttta gagatttcaa tgtgttgagc ggccctaatc aaagcacgag
     3481 catgcaattt ataaacggaa atatgtcgca gtccgtttca ttcacctatt atcttacagc
     3541 caacagtgaa gggacttttc gaattgaccc tgcaacaata accgttaacg gaggaaaagt
     3601 aaaatctaat acccttacaa taaatgttgt taaaagtcaa aaagcacaat cgggtgctca
     3661 acaacaaaaa agcaatgatg ctgatgcagg cattagcagc aatagtgtat ttttaagagt
     3721 gagtgtaaac aaatcatcgg tttacagagg agaagcactg ttagcgacat ataaat
//
LOCUS       DAORQT010000157         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4489_length_3762_cov_11.461289, whole genome shotgun sequence.
ACCESSION   DAORQT010000157 DAORQT010000000
VERSION     DAORQT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_3762_cov_11.461289"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PK134_06075"
     CDS             <1..1121
                     /locus_tag="PK134_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB38451.1"
                     /translation="SGVIISDDGYIVTNNHVVDNADKVDVTLDDKRTFTAKVIGKDPS
                     TDLALLKIDQTGLPYSKFANSDDIKVGQWVLAVGNPFNLTSTATAGIVSAKGRNIHIL
                     NDQQFPIESFIQTDAAVNPGNSGGALVNLNGDLLGINAAIASNTGSYTGYSFAIPANI
                     VRKVTKDLLEYGKVQRGFIGVTLRDIDQQLAQNKGLKSMQGVYIDGVVSGGAGQAAGI
                     KPGDVVTKVGETTVNSASELQEQIGRFGPGDKVNLTLNRDGTEKNYQIVLRDNSGGER
                     VERANNNVNMMGAEFGEVTDKEKKQLGISNGVKVAGLQSGKLRSAGIREGFIITSIDN
                     KKINSPDDIEAALANKKGGVLIEGVYPNGMRAYYGFGL"
     gene            1386..2747
                     /locus_tag="PK134_06080"
     CDS             1386..2747
                     /locus_tag="PK134_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB38452.1"
                     /translation="MLKKIVLTFLPLFISLLLNADTPLLKRVVCSYDDTTRLSFVSNA
                     NVFTERWDTLQQVKFWQEVIRQSADTALINVASTRCILHKTPFEDWKCQSESEKTAYK
                     KFINLANGLDTNTNLYVTFGKKEFYEYKRVIPVIGKSVEIFKNNKVDPWYAQTILLIE
                     NPGKNHNKSYVGANGPFQLMKSVAIKFGLKVNKHVDERTDLNKSAYAASQLLKSSCIP
                     KVKAMLNDKNIPFSEDDLWFRLLVMHAYHAGPGNVACVINELNPKKGGIDLFTQIWKT
                     ECGGFKNESQNYSQIALANIVIFERFLQSNKDSLWLVQGEKMMANYKKSKTQGIAGIN
                     KLKQSLELYSDDLMDGTIPADFFISRMTSIQKELAAYKIKKTNEEETNLSMTQYLALG
                     KQLIRKRQLDDAIKILKLNIQHYPNCVTAYDSLSRIYKIQGNKQMALFYNQKGASIKN
                     PAY"
     gene            complement(2757..3257)
                     /locus_tag="PK134_06085"
     CDS             complement(2757..3257)
                     /locus_tag="PK134_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRB38453.1"
                     /translation="MKKQVYTALTILMVFGVIKQTNAQEKVSIYHPEKNAFEQIKQAE
                     ITAQKEGKHIFLMIGGNWCRWCKMFDTFSKTDISVDSAFKANYVVEHINYSKENKNDS
                     LMQQLEFPQRFGFPVFVILDAHGKRLHTQSTGYLEKGEGYNKESVIEFLNQWSPAALN
                     PANYKK"
     gene            3355..3633
                     /locus_tag="PK134_06090"
     CDS             3355..3633
                     /locus_tag="PK134_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRB38454.1"
                     /translation="MRKVIVALLILFTACQPKSSEPEQKTAPALEEKEFPAKPDIALA
                     SDIDPVCKMSVKEEFSDTAMYDGKIYGFCASACKEDFLKEPLTYLEEK"
BASE COUNT         1267 a          612 c          709 g         1174 t
ORIGIN      
        1 gttccggtgt aattatttct gatgatggat atatagtaac aaacaatcac gttgttgaca
       61 atgcagataa agttgatgtt actttagatg ataaaagaac cttcactgca aaggttatag
      121 gaaaagatcc ttcaacagac ttggcattgc ttaaaattga tcagacaggg ttgccttatt
      181 caaaatttgc aaactcagat gatattaaag taggtcaatg ggtgttggcc gttggtaacc
      241 ctttcaatct tacttcaacc gctactgcag gtatcgtttc agcaaaaggc cgcaacattc
      301 atattttaaa tgatcagcag tttccgattg aatcatttat tcaaaccgat gcagctgtga
      361 atcccggtaa tagtggtggt gcattagtaa acctgaacgg tgatttgtta ggtatcaatg
      421 ctgccattgc ttccaataca ggttcatata ccggctattc ttttgccatt cctgcaaata
      481 ttgtaagaaa ggtaactaaa gatttattgg aatatggtaa ggttcaacga ggatttattg
      541 gtgttacatt acgcgatatt gaccagcaat tagcacagaa taaaggatta aaatctatgc
      601 agggtgttta tattgatggc gttgtgagtg gtggtgccgg acaagcagca ggaataaaac
      661 ccggtgacgt ggtaactaag gttggcgaga ctacagttaa ttctgcaagt gagcttcagg
      721 aacaaatcgg acgctttggc cctggtgata aagttaatct tacattgaat cgtgatggta
      781 cagaaaaaaa ttatcagata gtattgcgcg acaacagtgg tggtgaacgt gttgaacgtg
      841 ctaataacaa tgtcaacatg atgggtgctg aatttggaga agtgactgat aaagagaaaa
      901 aacaattagg cataagcaat ggtgttaagg ttgcaggttt acaatcagga aagttgagaa
      961 gtgcaggtat tcgcgaaggg tttataatta ccagcattga taataagaaa ataaattctc
     1021 ctgacgacat tgaagctgca ttagccaata aaaaaggtgg tgtgctgatt gaaggtgttt
     1081 atcctaatgg catgagagct tattatggat tcggattata gatagttttt cataggtttg
     1141 gtttatagcg aagggggatt ctgaaaatga atctcccttt gcattttatg caacttataa
     1201 attgcagtat ttctctcaca attcaaataa tagttaatac tcttgtgttg aaaacgaatt
     1261 ttgtttaact tgcagtggct taatatttct attttaaata gcctaccttt gcaaaagatg
     1321 caagaaaaca ataaaaaaag tctaaacagg ttatgttaat ttccgtataa ctataaatgc
     1381 aattgatgtt aaaaaaaata gtacttacat ttttaccact gttcatcagc ttgctgttaa
     1441 atgcagacac gcctttatta aaaagagttg tttgtagtta tgatgataca acccggttga
     1501 gttttgtttc taatgcaaat gtttttacag agcgatggga cacacttcag caggttaaat
     1561 tctggcagga ggtaatcaga cagtccgctg atacggcact gattaacgtt gcctctacac
     1621 gatgcatact tcataaaaca ccatttgaag attggaaatg tcagtcagaa tcagaaaaga
     1681 cagcctataa gaagttcata aacttagcca atggtcttga cacaaatact aacctttatg
     1741 ttacattcgg gaaaaaagag ttttatgaat acaaaagggt tattcctgta atcggaaaat
     1801 ccgttgagat ttttaaaaac aataaagttg acccttggta tgcacaaaca attctattga
     1861 ttgagaatcc cggaaaaaat cacaacaaat cttatgttgg agccaatggc ccttttcaat
     1921 tgatgaaaag tgttgctata aagtttggtt taaaagttaa caaacatgtt gatgaaagaa
     1981 ctgaccttaa taagtcagct tatgctgcat cacaactctt aaaaagctct tgtattccta
     2041 aggtaaaagc catgttgaat gataaaaata ttccatttag cgaggatgat ttatggtttc
     2101 gtctgcttgt aatgcatgcc tatcatgccg ggccggggaa tgtagcttgt gtcatcaatg
     2161 aacttaatcc taaaaaaggt ggcatagact tgtttactca aatttggaaa acagaatgtg
     2221 gtggatttaa aaatgaatcg caaaactatt ctcagattgc acttgccaac attgtgattt
     2281 ttgagcgttt tcttcagtca aataaagatt cactttggtt agtacaagga gaaaaaatga
     2341 tggctaatta taaaaaaagc aaaactcagg gcatagcagg aataaacaaa cttaagcaaa
     2401 gtcttgaact ctatagtgat gatttaatgg acggaacaat accggcagat ttttttattt
     2461 cccgaatgac aagtattcag aaagaacttg ctgcatataa aattaaaaaa acgaatgaag
     2521 aggaaactaa tctgagtatg acacaatatc tggcattagg aaaacaactt attcgcaaac
     2581 gccaattgga tgatgcaatt aaaattctga aattaaacat tcagcactac cccaattgtg
     2641 taactgcata cgactcatta agccgaattt ataaaattca gggtaataaa cagatggcct
     2701 tattttacaa tcaaaaaggt gcctctataa aaaatccggc ttattaaaat ttgaatttat
     2761 ttcttatagt ttgctggatt taatgcagca ggagaccatt gatttaaaaa ctcaattaca
     2821 ctttctttat tatatccctc tcctttttct agatagccgg tagattgtgt atgcaatctt
     2881 tttccatggg catcaagaat aacaaaaaca ggaaacccaa aacgttgcgg aaactccagt
     2941 tgttgcatca gtgaatcatt tttattctcc ttactgtaat taatatgttc cacaacatag
     3001 tttgctttaa atgctgaatc aactgatata tctgtcttac tgaaagtatc aaacattttg
     3061 caccaccgac accaattacc tccaatcatc agaaaaatat gttttccttc tttctgtgca
     3121 gtaatttctg cttgctttat ctgttcaaat gcattctttt caggatggta aattgaaacc
     3181 ttttcttgtg cattagtctg cttaatcact ccaaaaacca tgagtatcgt cagcgcagtg
     3241 taaacttgtt ttttcatact aaattttcaa ttgattcgtt taaaataata cctgaatcct
     3301 tcatctgata aaggttgtat ttaataactt tgtaaagttc aaataaacaa aaaaatgaga
     3361 aaagttattg tagcactatt aattcttttt acggcatgcc aacctaagtc ttcagagcct
     3421 gaacaaaaaa cagcaccggc acttgaagaa aaagaatttc ctgcaaaacc ggacattgca
     3481 ttagcatcgg acattgatcc ggtttgtaaa atgtcagtta aagaagaatt ttctgataca
     3541 gcaatgtatg atggtaaaat ttatggattt tgtgcatcgg catgtaaaga agattttctt
     3601 aaagaaccac tcacctacct tgaagaaaaa tgagttgtat tagttgtaaa agactttggt
     3661 caattcctgc tttggaatgg cttctaatat taatgactct tactttagga ttgttgtgga
     3721 catcgtgcaa tgaagatcgc ccattacgaa cattacctgt tt
//
LOCUS       DAORQT010000158         3721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4565_length_3721_cov_7.931806, whole genome shotgun sequence.
ACCESSION   DAORQT010000158 DAORQT010000000
VERSION     DAORQT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_3721_cov_7.931806"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PK134_06095"
     CDS             complement(<1..352)
                     /locus_tag="PK134_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRB38455.1"
                     /translation="MSELSKIYNPQETESKWYAHWMKEKFFKSVPDERPPYTIVIPPP
                     NVTGVLHMGHMLNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVQMLRDRGIK
                     KSDLTRDEFLKYAWE"
     gene            complement(474..2897)
                     /locus_tag="PK134_06100"
     CDS             complement(474..2897)
                     /locus_tag="PK134_06100"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38456.1"
                     /translation="MKFILIILAGITLLTKPVMGQTIQAKITDNNNEPLTGAIAELRL
                     TKDSTLNKVAVADATGKVIFDQIKPGSYFIKTTFLGFNNYFSPAFEFTEGENKDFRTI
                     KLSSSSTNLKQATITAFKPLVEVKADKTVFNVENSINSTGSTALELLQKAPGVVVDNN
                     DNIILKGRGGVMVQIDGREIRMTESELADYLRSIQSTDVESIEIISNPSSKYDAQGTA
                     GIINIKLKKNKNYGTNGSITAGYAVGTYSKYNTSLSVNNRTKKFNIYSTYSNNWGDRK
                     NEFYLYREQNPFTFNQSSKSKRGGLSHNYKAGADYQMNAKNSFGIMINGNYSDITGHQ
                     RNRNYISNFYSGVTDSILISNQRYNSINNNFYANLNHHYKDTTGTELTTDFDYGYYNG
                     TRNTYQPNEYTLPDGITPLSATYYKSNTPTTINIFTLKTDYSQSFFKGKLGAGYKVAL
                     VNTVNTFNFYNIEGGIATLDSSRSNKFDYTENVNAIYLNYQRTFNMIDVQVGVRMENT
                     NSNGKLKTSATANDKDVKRSYTDFFPSAGITYNINKDNSAGLIYSSRIDRPNYQELNP
                     FEYKLDELSFRKGNPFLNPQYSNKIELTHTYKYIITTSAGYSHTKDFFAQITDTLGEG
                     KSYLMPRNLATEDVLSISLSASLQPFKWYSIYLNASVDNQKYKADYGGNKTINAQFTS
                     FNLYGQNTFKLPYQFTFELSGWYNSGGVWGGSYKTKAQGSLDVGLQKKLLKDQATLKI
                     SYTDLLHTAPWDSYNTYAGIVSRAWGNWESQQLRVSFTWRFGNKQMKNIRQRSSGSEQ
                     EQKRIGGGD"
     gene            complement(2949..3641)
                     /locus_tag="PK134_06105"
                     /pseudo
     CDS             complement(2949..3641)
                     /locus_tag="PK134_06105"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
BASE COUNT         1056 a          650 c          692 g         1323 t
ORIGIN      
        1 actcccaggc atattttaag aactcatctc ttgtaaggtc agatttttta attcctctgt
       61 cgcgcaacat ttgcacaacc ttagcttctg ttgcaataga agcatgatct gttcccggaa
      121 cccagcatgc atttttacct aacatgcgtg ctctgcggat cagtacatcc tgtatggtgt
      181 tgttgagcat gtgacccatg tgcaacactc ctgttacatt tggtggagga ataacaatag
      241 tgtagggcgg acgctcatca ggaaccgatt taaaaaattt ttctttcatc caatgtgcat
      301 accatttgga ttccgtttcc tgtgggttat atatttttga taattctgac atgtgcttta
      361 aaaagtgtga atgcaaaaat aagaaattag ctatgtgcaa aacgaacaaa gcctgctata
      421 aaagcaggct ttgaaaaata agtgtacttt ctttctgtaa aaggaggact ttgctaatcg
      481 cctcctccaa ttcttttttg ttcctgctca ctaccactgc ttcgttgtct gatgtttttc
      541 atttgtttgt tgccaaatcg ccaggtaaac gacaccctga gttgctggct ttcccaattg
      601 ccccatgcac gactcacaat gcctgcataa gtgttgtaac tgtcccatgg agctgtatgg
      661 agtaagtcgg tatatgaaat ttttaatgtt gcctgatctt tcaataattt tttctgtagg
      721 cccacatcta atgatccctg tgcctttgtt ttgtatgagc caccccaaac acctcctgag
      781 ttgtaccatc ccgaaagttc aaatgtaaac tgatagggta acttaaatgt gttttgacca
      841 tataaattaa atgaggtaaa ctgtgcattg atggttttat tacctccata atcggctttg
      901 tatttttgat tgtcaacgct tgcattcaaa taaatactgt accatttgaa aggttgtaat
      961 gatgcactta aactaatgct caacacatct tctgtagcca gattgcgagg cattaaatag
     1021 ctttttcctt cgccaagtgt gtcagtaatt tgtgcaaaaa agtcttttgt gtgactatat
     1081 ccggccgatg ttgtaatgat atatttataa gtgtgtgtaa gttcaatttt atttgaatat
     1141 tgtgggttta aaaatggatt tccttttctg aaacttaatt catcaagctt atattcaaac
     1201 ggatttaact cctgataatt tggtctgtca attcgcgaac tgtaaattaa tcctgcagaa
     1261 ttatccttgt tgatattgta ggtgatgcct gcgctgggga aaaagtcagt atagcttcgt
     1321 ttcacatctt tatcatttgc ggtggcagag gtttttaatt tgccattact attcgtattc
     1381 tccatgcgga cacctacttg cacatcaatc atattgaatg tgcgttggta atttaaataa
     1441 atggcattca cattttctgt ataatcaaat ttattgctac gtgaagaatc caatgttgca
     1501 ataccacctt caatgttgta aaaattaaat gtgttgacag tgtttaccaa agcaacctta
     1561 tatccggctc ctaacttgcc cttaaaaaac gattgcgaat aatctgtttt taaagtaaag
     1621 atattgatag ttgttggtgt atttgacttg taatatgtcg cagaaagtgg tgtgattcca
     1681 tcaggcaatg tgtattcatt tggttgatac gtgtttcttg ttccgttgta gtagccgtaa
     1741 tcaaagtctg ttgtcaactc tgttcctgtt gtgtctttgt aatgatgatt tagattggca
     1801 tagaaattat tgtttataga gttataacgt tgattggaaa taaggatact gtctgttact
     1861 cctgaataaa aattgctgat atagtttctg tttctctgat gtccggttat gtcgctgtaa
     1921 tttccattta tcataatgcc aaaactattt ttagcattca tctgataatc tgcaccggct
     1981 ttgtaattat gacttaggcc accacgtttc gattttgatg attgattgaa tgtaaacggg
     2041 ttttgttcgc gatacagata gaactcgttt tttctgtcac cccaattatt actgtaagta
     2101 gagtaaatat taaacttttt tgttctgttg ttgactgaaa gtgaggtgtt gtatttagaa
     2161 tatgttccaa cggcataacc tgctgttata cttccgttgg tgccatagtt tttgtttttc
     2221 tttagtttaa tattgatgat tccggccgtg ccttgcgcat catatttgga tgatgggttt
     2281 gaaataattt caatactttc cacatctgta ctttgaatag aacgaaggta gtcagcaagt
     2341 tcgctttctg tcattctaat ctcacgtcca tcaatctgaa ccatcacgcc accacgtcct
     2401 tttaaaataa tgttatcgtt attatctaca acaacaccgg gcgctttttg aagcaattca
     2461 agtgcagtac ttcctgttga gttaatactg ttttctacat taaatacagt tttatctgct
     2521 ttaacttcaa ccaagggttt aaatgcagtg atggttgctt gcttaagatt agttgatgat
     2581 gatgaaagtt ttatagtgcg aaaatctttg ttttctcctt ctgtaaattc aaatgcagga
     2641 ctaaaataat tgttgaatcc caaaaacgtt gtctttataa aatagctacc gggttttatc
     2701 tggtcgaaaa ttacttttcc tgtagcgtca gctactgcaa ctttgtttaa ggttgaatct
     2761 ttagtgagtc gcagttctgc aatggcacct gtaagcggct cgttattatt gtctgtgatt
     2821 ttagcctgaa tagtttgacc cattacaggt tttgtcaaca gggttattcc tgccaagata
     2881 attagaataa atttcattgt ttgtgtgtta tggttgcggg aaaatatagt attgctgcta
     2941 tgtattattt agttttctga tgaaatatta ttttttctta attgtaaata atcgagataa
     3001 taaagtactt taattgaaaa tgtttgaaca tgcggatttt taaaaacata atcaagattt
     3061 ttagtgaatg atgcagtttc aagtttgcca tcagtaccat tgtcaaaaat gtttttgtac
     3121 gaaaaagtca atgtgctgcc gggagcaaat cgccatgaaa acaaaaggtc agcattaaaa
     3181 aaattatagc tgccatcaaa ttcgttgttg taaatatcgg ttgtgacagc aagcccaccg
     3241 tcatcctgca gcaaaaagta tttcctatat gcgtaattaa accagtaatg ccgtgcaaca
     3301 attgacagcg acagatcatt tttaaatata tatcgtgctg caagactgtt tatataggta
     3361 ttggttcttc ttaacccaaa aaggtaaata ttcgggtcag tttcacttgt aaaaccgaaa
     3421 ttatagggat caaaattgta ttccgaagaa gcattaactg tgagtttgtc attaatgcga
     3481 taacgtaagg tagcatccgc gcttccgcct tcggaaagat aggttttaat aaagttactg
     3541 atattaattt ccatatctaa tgctaatgcc cttctgtaat ctgtactaac tccgatatat
     3601 gcaaaccagt attttaacgc attaacataa cgcccattta gtcttggatc atattcacgt
     3661 ccggctgcag ggcattatat tggagaggac aggtttgttg taagaaaaaa aattacaaaa
     3721 g
//
LOCUS       DAORQT010000159         3651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4708_length_3651_cov_8.725528, whole genome shotgun sequence.
ACCESSION   DAORQT010000159 DAORQT010000000
VERSION     DAORQT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4708_length_3651_cov_8.725528"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..648)
                     /gene="ribH"
                     /locus_tag="PK134_06110"
     CDS             complement(145..648)
                     /gene="ribH"
                     /locus_tag="PK134_06110"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRB38457.1"
                     /translation="MALYQENNSLYNPAEYPDASSVKVGIVTASWNNKITSKLFDGAA
                     DVLKKSSVKENNLITIQVPGSYELTAGAQWLFQQHAPDVVIALGCVIQGETRHFDFIC
                     NAVANGLNHVSLKFGKPVIFGVLTTDNEHQALERCGGTHGHKGCEAAFTALSMALLKT
                     PVAKIGF"
     gene            complement(655..1329)
                     /locus_tag="PK134_06115"
     CDS             complement(655..1329)
                     /locus_tag="PK134_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38458.1"
                     /translation="MTDKKSTGFDVEESLDRAESYIHENKKSLTIILVVAVVLVGGYF
                     AYNQFIVKPQQANAEKQMFMAEYYFKNDSLDKAIKGDGNFPGFEEIISSYGSSKSANL
                     AHYYLGVSLLRKGQYDDAITALSKYDAEDDITGAIALGCIGDAYMEKGNKEEAMNFYK
                     KASDYDQNQFTAPIYLMKQAMVLELNNDYKSAYDIYNRIKRDFPNSTEARQIATYMAR
                     AEAHMN"
     gene            complement(1419..2294)
                     /locus_tag="PK134_06120"
     CDS             complement(1419..2294)
                     /locus_tag="PK134_06120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRB38459.1"
                     /translation="MSVPLRTQLFNVFRLVNRIPKVEKWLVKQSKSKNSFARKLIGNQ
                     NLYSPKDIRICTRHGINYQLQINDYLDHGIYFGMHDLSDFSRTSLLKNIQAGMIVFDI
                     GANIGDSVLQIAKKLNGTGSIYAFEPSPDVFQRLNRNVSLNNFNNIETFNLGMADVES
                     QLSFVVEDKNHSGGAFISKDANNAIKVAVTTIDKFVAEHNLSKLDFLKIDTEGFEVFV
                     LKGGVNTFRNFKPKMFIEVSDSLLKRAGSSAKELIQILNELNYHSVRVDTQENITEDY
                     NFAEQHFDIYCTPLT"
     gene            complement(2426..>3043)
                     /locus_tag="PK134_06125"
     CDS             complement(2426..>3043)
                     /locus_tag="PK134_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38460.1"
                     /translation="TFLSVSEPMSDSMIANMFPSGKFQCWDTDVVYSYFRLLGSNRLL
                     LGGGDMLTTFSKQDVNSSIVIDRVIRKFKQKFPQLSNLEFVQYWPGRIDCTRDLLPTI
                     ARDENKKWLHFVLGCVGLPWATFCGDFAARHAYDDSACDDHHYYDYLRPDRGFFVPLW
                     LEKILSKQIVFSLNNGWAKYYQVDINSAETKRITQKREAENSNMN"
     assembly_gap    3044..3053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3054..>3651)
                     /locus_tag="PK134_06130"
     CDS             complement(<3054..>3651)
                     /locus_tag="PK134_06130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB38461.1"
                     /translation="DKKLKVVILEKNICGGDTSGKSAGFLTPDSELELSQLIRRFGIT
                     GAADLWKIATTGVKMIHHIIETNKIGCDFQVQDSLFLGIGKSGLQDIIEEMESRKLLK
                     FDQQIYTKENIKSIIGSEAYSGAVRYTGTFGIDALLYCQGMKKLLLENGIEIYESSEV
                     VKIEDHCVRTHLGSVKADQVIFCADKLQHELTHYADKVY"
BASE COUNT         1141 a          718 c          591 g         1191 t
ORIGIN      
        1 aggcatgata atcgagtgct tctttattaa ctggcataat tatttttttt acaaagtgct
       61 aaagtaaaga atacaacatc acgtcattgc agaaaataaa cagatgagat actgaattta
      121 atcagcttaa aataaattta aaatttaaaa accgattttg gcaacaggag ttttgagaag
      181 tgccatgctc aatgcagtga atgcggcttc acacccttta tgtccgtgag tgcctccaca
      241 acgttcaagt gcttgatgtt cgttgtcggt agtgagtacg ccaaaaatta caggcttgcc
      301 aaattttaac gaaacatgat tgagtccatt ggcaacagca ttgcagataa aatcaaaatg
      361 acgtgtctcg ccctgaatga cacaacccag tgcaataaca acatcaggag catgttgttg
      421 aaaaagccat tgtgcacctg cagtaagttc atagctaccc ggcacctgaa tagtgatgag
      481 gttattttct tttacagaag attttttaag gacatcagcg gctccgtcaa aaagttttga
      541 ggtgatttta ttattccaac ttgctgtaac aattccaact tttaccgaag atgcatcagg
      601 atattcagca gggttataga gagaattatt ttcctgatat aatgccatga aaaattagtt
      661 catgtgtgct tctgcacgcg ccatgtaggt tgcaatctga cgtgcttcag tagagtttgg
      721 gaagtctctt tttattctgt tataaatatc gtaggcagat ttatagtcgt tgttcaactc
      781 taaaaccatt gcctgcttca tcagataaat aggcgctgtg aattgattct gatcataatc
      841 tgaagccttt ttataaaagt tcatagcctc ttctttattg cctttttcca tgtacgcatc
      901 accaatacat cctaaagcaa tagctcctgt aatatcgtct tctgcatcgt atttagataa
      961 agcagtaata gcatcatcat actgtccttt gcgcaacaaa cttacaccaa ggtagtagtg
     1021 tgccagatta gctgactttg atgaaccata gctggaaata atttcttcaa agccgggaaa
     1081 atttccatca cctttgatgg ccttatccaa agaatcgttt ttgaaataat attcagccat
     1141 gaacatttgt ttctcggcat tagcctgctg cggctttacg ataaattgat tgtatgcaaa
     1201 atatccgcca accaatacca ctgcaactac aagaattatg gtcagacttt ttttgttttc
     1261 gtgtatatag gattcagccc tatctaagga ttcttctaca tcaaaaccgg ttgatttttt
     1321 atcagtcatt gtttctgcaa aaatttgagc gggcaaagat agtttttttt ctgaaatatc
     1381 aggcattcta ttataacgcc taattagctc aaattcaact atgtaagagg tgtgcaataa
     1441 atatcaaaat gctgttcggc aaagttataa tcttccgtta tgttttcctg agtgtccact
     1501 cttacactgt ggtaattcag ctcatttaat atttggatga gctcttttgc agaagagcct
     1561 gctcttttaa gtaatgaatc gctgacttca ataaacatct taggcttaaa attccgaaaa
     1621 gtatttacac cacctttaag aacaaaaact tcaaatcctt ctgtgtctat tttcagaaaa
     1681 tcgagttttg acagattatg ttcagcaacg aacttatcaa ttgttgtgac agcaactttt
     1741 atagcattgt ttgcatcctt actgataaat gcaccacctg aatggttttt gtcttcaact
     1801 acaaacgaca actgactttc aacgtcagcc atacctaaat taaaggtttc aatattattg
     1861 aaattattga ggcttacatt tctattcagt cgctgaaaaa catctggcga aggttcaaag
     1921 gcataaattg atcctgtgcc atttaatttt tttgcaattt gtaaaaccga atctccaatg
     1981 ttagcaccta tatcaaaaac tatcattcct gcttgaatgt ttttaagcaa acttgtacga
     2041 ctaaaatcac tcaagtcgtg catgccaaaa taaattccgt ggtcgagata atcatttatt
     2101 tgaagttgat agtttatacc atgtcgtgta caaatacgga tatctttcgg gctatataaa
     2161 ttctggttac caattaattt acgcgcaaaa ctatttttgc ttttcgattg tttgaccagc
     2221 catttttcaa cttttggaat ccgattcact aaacgaaaaa cattaaataa ttgtgttctt
     2281 aatggcactg acatagcaat ataaatcttt cgcaaatata ttgtgtaaga ttgaaaaaca
     2341 gtacttcaat ctgtcatttt tcttttactc taacctcgaa aatagtcagt agcaatattt
     2401 taagattcaa ttgcatcagt aattctcaat tcatattgga attctcggcc tcacgtttct
     2461 gtgtaattct cttagtctct gcagagttta tatctacctg ataatacttt gcccaaccat
     2521 tgttgagtga aaaaacaatt tgtttgctta ggattttttc aagccaaagc ggaacaaaaa
     2581 atcctctgtc aggacgcaga tagtcataat aatgatgatc gtcacatgca ctgtcatcat
     2641 aagcatgtcg tgcagcaaag tcgccacaaa aagttgccca aggaagtcct acacaaccta
     2701 aaacaaaatg caaccatttt ttattttcgt cacgtgcaat agttggcaat aaatcgcggg
     2761 tacaatctat tcttccgggc caatactgaa caaattcaag gttacttaac tgaggaaatt
     2821 tctgtttgaa ttttctgata acgcgatcaa taacaattga agagttgaca tcctgttttg
     2881 aaaaagtagt cagcatatca cctccaccca acaacaaacg gttgcttcct aaaagtctga
     2941 agtaggaata caccacatca gtatcccaac actgaaattt acctgatgga aacatgttag
     3001 caatcatgga atcgctcatg ggttcgctta cggaaaggaa tgtnnnnnnn nnngatatac
     3061 tttgtctgca tagtgtgtta attcatgctg caacttatct gcacaaaaaa taacctggtc
     3121 tgctttaact gagcctaagt gtgttctgac acaatgatct tcaattttta caacttctga
     3181 cgactcataa atttcaatac cattttcaag taacagtttt ttcatccctt ggcaatacaa
     3241 aagtgcatca ataccaaaag tgcccgtata acgaactgcg cctgagtatg cttctgaacc
     3301 aataatactt ttaatgttct ctttggtata aatttgctga tcaaacttca ataattttct
     3361 ggattccatt tcttcaatga tgtcttgcaa gccacttttg ccaataccta aaaacaaact
     3421 atcctgcacc tgaaaatcgc aaccaatttt attagtctca ataatatgat gtatcatttt
     3481 tactcctgta gttgcaattt tccaaaggtc agctgcgcct gtaatcccaa atctacgaat
     3541 caactgtgac aattctaatt cactgtcggg agttaaaaac ccagcacttt taccagacgt
     3601 atcgccacca caaatatttt tttcaagaat aactactttt agttttttat c
//
LOCUS       DAORQT010000160         3604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4805_length_3604_cov_6.233305, whole genome shotgun sequence.
ACCESSION   DAORQT010000160 DAORQT010000000
VERSION     DAORQT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_3604_cov_6.233305"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="PK134_06135"
     CDS             complement(<1..2012)
                     /locus_tag="PK134_06135"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRB38462.1"
                     /translation="MWCLAVIVFSTENIHAQTLPKDSLSTDTLAKDTIKTGVSNDLKS
                     KVHYTADDTIEFDIEHEKVFLYEKAQVNYENIELKAAYIEVDWNTKNVFSAGLRDSTG
                     KLYGMPVFKEGDDEFKADTMRYNFDTKKGKVYHVVTQQGDGFIHGAIVKKIEESSYIR
                     YGKYTTCSLDTPHYYIAANKLKVIPNNKIITGPAYLIIGDVPAPAAIPFGLFPNTKGR
                     SSGILFPAYGESNALGFFFKNGGYYFGISDHVDLQLTADIYTLGSWAVKATSRYNNRY
                     HYNGNLSFLYSIIKVSEKDLPDYSRSKEFFINWSHLQDPKARPNGIFSASVRAGSNDY
                     YTRNISSASNYLTNTFNSSISYSRSFFNKQLNFSSAITHSQNTQTRMVYLTAPSINLS
                     MNTLYPFRKKEQEGTLAWYEKISVGYSSTLQNQLQNPDSLFCKNTTLQQMKNGMQHNI
                     PISASFKAMKYFNLTPAINFNERWYIETYKQRFDADSNRIMTDTVKGFNAAHEYNGSL
                     SLNTRIYGMKQFKKGKIAAIRHVLSPTLSYMYKPDFGENKYGYYKTVQTDSIGNSRRY
                     SIYENTLYGGPSQGKSNILSFSLDNNLEMKTRQYTDTAVNIKKIKILESFGLSASYNL
                     SADSFNLSPIGMNARTTLFDMVSLTASGTFDPYLMNDQGIRIAKYSL"
     gene            2211..3059
                     /locus_tag="PK134_06140"
     CDS             2211..3059
                     /locus_tag="PK134_06140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRB38463.1"
                     /translation="MSASNGTYKEPYRIKTIVIDPGHGGHDSGCLGASSKEKQIALEV
                     ALKLGEMITQKYPDVKVIYTRKTDEFVELHERAAIANRNHAELFICIHCNSGSPAAFG
                     AETYVMGLHKTEDNLNVAKRENAAVLLEKDYKQKYDGFDPNSPEANIVFTLYQNAYLT
                     QSLKFASAVQQQFEVYAGRYNRGVKQAGFLVLYKTAMPSVLIETGFLTNRTEEKYLLT
                     EKGQNAVATSIFRAFMEYKLDMETPGGKIPQGGSENDLNKNVIEVKNKQSDKESSQQA
                     EVLALI"
     assembly_gap    3308..3345
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1096 a          623 c          694 g         1153 t
ORIGIN      
        1 agagaatatt ttgctattcg tattccctga tcgttcatca gataggggtc aaatgttcct
       61 gaagctgtta aactaaccat atcaaaaaga gttgttcgtg cattcatgcc aataggcgag
      121 aggttaaatg aatctgccga caaattgtat gatgctgata gtccaaaact ttctaaaatt
      181 tttatctttt taatgttcac tgccgtatca gtatattgtc tggttttcat ttccagatta
      241 ttgtctaagc taaaactaag aatgtttgac ttgccttgcg aaggaccacc atacagtgtg
      301 ttttcataga tggaataacg tctgctgtta ccaatgctgt ctgtttgtac ggttttgtaa
      361 taaccatatt tgttctcacc aaaatccggt ttgtacatat agcttagtgt tggtgaaagc
      421 acatgtctta ttgcagcaat ttttcctttc ttgaattgct tcatgccata aattcttgtg
      481 ttcagactta atgaaccatt atactcatgt gcagcgttaa atcctttaac cgtatcagtc
      541 ataattctgt tgctgtcagc atcaaatcgt tgcttgtatg tttcaatata ccaacgttcg
      601 ttaaaattaa tggccggtgt aagattaaaa tatttcatcg ctttaaatga tgcactgatt
      661 ggaatgttat gttgcattcc gtttttcatc tgctgcagtg tggtgttttt gcagaataga
      721 ctatcgggat tttgtaattg attttgtaat gtactactgt agccaacact tattttttca
      781 taccaggcaa gcgtgccctc ctgctctttt ttgcggaaag gatacagtgt attcatgcta
      841 aggtttatac ttggggcagt taaataaacc attcgtgttt gcgtgttttg cgaatgtgtg
      901 atggcagatg aaaagttaag ttgtttattg aaaaaagaac gtgaataaga aattgatgag
      961 ttgaaagtat tagtaagata gtttgatgcg cttgaaatat ttcttgtgta ataatcgttg
     1021 gacccggcac ggacagaagc ggagaaaatt ccgttaggcc ttgctttagg atcttgcagg
     1081 tgactccagt ttatgaaaaa ttctttgcta cggctatagt ctggtaaatc tttctcactt
     1141 acttttatga tagagtatag gaacgataaa tttccattgt aatgataacg gttattgtat
     1201 cgtgaagttg cttttacagc ccaactacct aaagtataga tgtctgctgt aagttgcagg
     1261 tcaacatggt cgctaatacc aaaataatag cctccgtttt taaagaaaaa tccaagtgca
     1321 ttgctttcac cgtatgcggg aaataaaatt ccggaactac gacctttggt attgggaaat
     1381 aatccgaatg gaatggctgc aggtgcagga acatctccaa taatcagata agcaggcccg
     1441 gtaataattt tattgtttgg aattactttg agtttgtttg cggcaatgta atagtgtggt
     1501 gtatccaaac tacaggtagt gtatttgcca taacgtatat agctgctctc ctctattttt
     1561 ttaactattg caccatgaat aaagccatcg ccctgctgtg ttacgacatg ataaaccttg
     1621 cctttcttgg tgtcaaaatt atagcgcatt gtatcggctt taaactcatc atcaccttct
     1681 ttaaaaacag gcattccata gagttttcct gttgaatcgc gcaatccagc cgaaaaaaca
     1741 ttttttgtat tccagtccac ttcaatataa gcagccttca gctcaatatt ttcataattg
     1801 acctgagcct tttcatacaa aaaaactttt tcatgttcaa tatcaaattc aatagtatcg
     1861 tcagcagtgt aatgaacttt ggactttaaa tcattactca caccggtttt aatggtgtct
     1921 ttggcaagag tatcagtaga taatgagtct tttggaagcg tttgtgcatg aatattttca
     1981 gttgaaaaca caatgaccgc caggcaccac aatattgcaa tcgttaataa gtttttaaaa
     2041 acggtcattt acaaaaaaag gagatggagt taaaaacgat atttttggag ccgcaaaggt
     2101 aattgaacag aatttaacac catccaaaga aacgcagaat catgttgttt atttataaga
     2161 agataaaaat acggctcttt tttacacttt cactgttgtt tttaatctgt gtgtcagcgt
     2221 ctaatggaac atataaggaa ccatatcgca taaagaccat tgttattgac cccggtcatg
     2281 gtgggcatga ttcaggctgt ctgggtgctt catctaaaga aaaacaaata gcacttgaag
     2341 tggcacttaa attgggtgaa atgattaccc aaaaataccc tgacgtgaag gttatttaca
     2401 caagaaaaac agatgagttt gtagaattgc atgagcgtgc tgctattgcc aatcgtaatc
     2461 atgccgaact tttcatctgc atacattgca actccggtag cccagcagct tttggtgcag
     2521 aaacctatgt tatgggttta cataaaacag aagataatct taatgttgcc aaacgcgaaa
     2581 atgccgctgt gttgttggaa aaggactaca agcaaaagta tgatggcttt gaccccaact
     2641 cgcctgaagc caatattgtg tttacattgt atcagaatgc ttatctgact cagagtttga
     2701 agtttgcctc tgccgttcaa caacagtttg aagtctatgc agggcgttat aaccgtggtg
     2761 ttaagcaggc tggttttttg gtactttata aaactgctat gccatcagtt ctgattgaaa
     2821 ccggattttt aaccaacaga acagaagaga aatatttact tactgaaaaa ggtcagaatg
     2881 ctgttgctac aagtattttc agagcattta tggagtataa attagatatg gaaactcctg
     2941 gcggcaagat accgcaggga ggctcagaga atgacttaaa taaaaatgta attgaagtaa
     3001 agaataagca atcagataag gaatcttctc agcaagcgga agtgttggct ttaatctgaa
     3061 ccgacagaaa ggaaataaga aaagtttaaa aggaactgtc gaacaggtaa aagaaattaa
     3121 tgatcatcct gatgattatg ttgacttttc tgtaccatat aatttatcgg tcaattacag
     3181 cattgcctac tcacgaccaa ctaatgaaaa aggtaatctt tcgcagatag ttggtttcaa
     3241 tggcgacatt tcactgactg aacattggaa aataacattt aactccggtt atgattttca
     3301 aaccaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttatc gcgatttgca
     3361 ttgctgggaa atgcgtttaa actgggtgcc tttcggatat caggccaact attattttca
     3421 aatcaatgtt aagtcaagtg tacttcagga tttgaagctg acgaagaaaa acgaccgcta
     3481 cgataatttc taaaattact caacactgaa tttatgtcct tcaatagcaa gttctgtatt
     3541 tttaaatacc gttcttgctt catcgagcaa tttatctgaa acagcataaa tgtcagcgcc
     3601 tttg
//
LOCUS       DAORQT010000161         3582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4850_length_3582_cov_4.265665, whole genome shotgun sequence.
ACCESSION   DAORQT010000161 DAORQT010000000
VERSION     DAORQT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4850_length_3582_cov_4.265665"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PK134_06145"
     CDS             complement(<1..1192)
                     /locus_tag="PK134_06145"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB38464.1"
                     /translation="MAIKLMDSTLVNYTRSDEKGFFNINALPVDTYQVIITHPKFADM
                     GFFIFGDSTNLEYDFGKIILQPKNIDLHEVTIYAFKDPVYYKGDTLIYTADSFKTKAN
                     ANVEDLLKKLPGLSVDASGKITSQGKSIDKVLVDGDEFFGSDPTMATKNLAASSIESV
                     QVYEKKSDDAANSSTGEETQNILNLQLKEEAKKGYFGKISGASDFKKFHEGEMLGNYF
                     KNKLKVSVFGLASNTPNSSFNWEDVYRYGLDNEFQTVFDDDGGSTSMFMTGQPQGIPQ
                     TIKSGFYYSNKLFKDTKFNINYSYDNSQVNATKSENSQYFLPDTTYNTFNNTNAFQKN
                     EAHTINIGLEQALDSLTDLNFSSKIKSIEINSTSLDKTDFLRSDFIKTRNTTIDNFSK
                     SNGYD"
     gene            1573..2022
                     /locus_tag="PK134_06150"
     CDS             1573..2022
                     /locus_tag="PK134_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38465.1"
                     /translation="MKLFNYVMTAALVSAVLFTSCKKKDDDNASPNPPAQSAGFYYGE
                     NGTSTLTKADSSWVNGAFNTIIAQTGGSTVVEINLTGITAQGYSLGTTNSLTYIKNGS
                     FWATTAGTVTITKNQDSKLSGTFSATAGGGVSGVTSFSGNFTDIPIK"
     gene            complement(2141..2887)
                     /locus_tag="PK134_06155"
     CDS             complement(2141..2887)
                     /locus_tag="PK134_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015273866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRB38466.1"
                     /translation="MIKIISISILSMLLFAITQPNDFLAEQKKFIRVRTAIEEKQSFI
                     EEKLKEHKITSDNLNILIVAYKEDDLLDVYAKTIQETTYKKILSYNICSRSGQPGPKR
                     KQGDGQVPEGFYHINRFNPTSSFYLSLGINYPNLADKRKSNSSNLGGDIFIHGSCVTI
                     GCLPMTDNYIKEIYLISVLAKNCGQSKIPVYIFPFKMSDQNLTTYCAKYKENKGLISF
                     WNNLKTGYDKFIKTQKELAIGVNENGDYIY"
     gene            complement(2946..>3582)
                     /locus_tag="PK134_06160"
     CDS             complement(2946..>3582)
                     /locus_tag="PK134_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRB38467.1"
                     /translation="RQAFKLMKFHARTIAFLSGINVKVIRKYEPMENETYIICPNHIS
                     YLDIVLTYVAFPHYFHFMGKAELERVPFFNIFFKRMNIGVNRSSRMASHKAYQRACKD
                     LSQNISISIFPEATIPDCNPQLGPMKNGAFKLAIENQIMLLPVCYIDNWKILPDCPRK
                     ILGGRPGIARVIIDKPIEVKGLTEADVPELKKRYRERMDNILQEYQYGYGK"
BASE COUNT         1100 a          609 c          588 g         1285 t
ORIGIN      
        1 tatcataacc attacttttt gaaaaattat caattgttgt attacgtgtt tttataaaat
       61 cacttcttaa aaagtctgtt ttatccaaac tggtagaatt gatttctatt gatttaattt
      121 ttgaagaaaa gtttaaatct gttaatgagt ctaacgcctg ctctaagcca atgttaattg
      181 tatgtgcttc atttttttga aaagcgttag tgttgttgaa ggtattgtat gtagtgtcag
      241 gtaaaaaata ttgtgagttt tctgatttgg tagcattaac ctgactgttg tcgtaggagt
      301 aattaatgtt gaatttagta tctttaaaaa gcttatttga ataataaaat cctgatttaa
      361 tagtttgcgg aattccctgt ggttggcctg tcataaacat tgaagttgag ccaccatcat
      421 catcaaagac tgtttgaaat tcattgtcta gtccatatct ataaacgtcc tcccagttaa
      481 aacttgaatt tggtgtgtta cttgccagac caaacaccga aacttttaac ttatttttga
      541 agtaattacc taacatttca ccttcatgaa atttcttgaa gtcgcttgct ccacttattt
      601 taccaaagta tcctttttta gcttcctcct taagttgtaa atttaagatg ttctgagttt
      661 cttctcctgt acttgaatta gctgcatcat cacttttctt ttcatatacc tgaacagatt
      721 ctattgaact tgctgctaag ttttttgtag ccatagttgg gtcgcttcca aaaaactcat
      781 caccatccac caagactttg tcaattgact ttccctgaga agtaattttt cccgatgcat
      841 ctacggataa tccgggtaac ttctttaaca aatcttcaac atttgcatta gcttttgttt
      901 taaatgaatc tgccgtataa attaacgtgt cgcccttata atagacggga tctttaaaag
      961 cataaatcgt tacttcatgg agatctatat ttttaggttg aagaataatt tttccaaagt
     1021 cgtattctaa attagtactg tctccaaaaa taaaaaaacc catatcggca aactttgggt
     1081 gtgttataat tacctgataa gtatcaacag gtaatgcatt tatattaaaa aagccttttt
     1141 catcactacg ggtataatta accaaggttg aatccatgag cttaatagcc atgattaaag
     1201 catttgaaag tggttctctg gcagtggtat cctgaacatt tcctttaact gatatctttg
     1261 agcgaaagtc gagtaggtgc tgagtaatag aaaccagaat actaattttt tcattaagtc
     1321 atctttatag tgacgatagt tttagctata atattacaaa aatattattt ttaaggtgga
     1381 tatttaaaca tcaatggaga aacttgttaa aggttttgta tctgatgaga ttgagtaggt
     1441 ttttgaaaaa ggtttagtta ttaacaattc attttttcta atttttcaaa atgggctgaa
     1501 cagcccattg ctattgcgta accagcatga gaattttgcg tttcaagcaa tataatttaa
     1561 acacacaaaa aaatgaaact atttaattat gttatgactg ctgcattagt gtcggcagtg
     1621 ctatttactt cttgcaaaaa gaaggatgat gacaatgcct cacctaatcc acctgcacaa
     1681 agtgccgggt tttattacgg tgaaaacgga acttctacac tcaccaaagc cgactcatca
     1741 tgggttaatg gtgcattcaa tactatcatt gcccaaacag gcggaagcac tgtagtagaa
     1801 attaatttga ccggcattac agcacaaggt tatagtttag gtacaacaaa ttcgctaacc
     1861 tatattaaaa atggcagctt ttgggctaca acagcaggta ctgtaacaat tacaaaaaat
     1921 caggatagca aactttccgg aacctttagt gccactgcag gtggaggcgt tagcggtgtt
     1981 accagctttt cgggtaactt tactgatatt ccaattaaat aattttcgga attgtcatgc
     2041 agcaacattg ttgcatgaca atttttttat ctcagcctaa aacctgaatt ctgaaattta
     2101 aaacgcaaaa ttatttaagc tgcattttgg caacagtttt ttagtatatg taatccccat
     2161 tctcatttac acctattgct aactcttttt gagtttttat aaatttatca taacccgttt
     2221 tcaagttgtt ccagaatgaa attaaccctt tgttctcttt gtatttggca caataagtag
     2281 ttaagttttg gtcagacatt ttaaacggga aaatataaac cggtatttta gattggccgc
     2341 agttttttgc aagaactgaa attaaatata tttctttaat gtagttgtca gtcattggta
     2401 agcaaccaat agttacacac gatccatgaa taaaaatatc accacctaaa ttacttgaat
     2461 tactttttct tttgtctgca aggtttggat agtttattcc aagtgacaaa taaaaactac
     2521 ttgttggatt aaatctgttt atgtgataaa atccttcagg cacttgccca tcaccttgtt
     2581 tcctttttgg tcccggttgt ccggaacggg agcaaatatt gtatgaaaga atttttttat
     2641 aagttgtttc ttgtattgtt ttagcgtaaa catcaagcaa atcgtcttct ttataggcaa
     2701 caataagtat gttcagatta tcagaagtga ttttatgttc ttttaatttt tcttcaataa
     2761 aactttgttt ttcttctatt gcagtcctta ctcttataaa tttcttttgt tcagcaagga
     2821 aatcatttgg ctgtgtaatg gcaaataaca gcattgataa tatacttatg gaaattatct
     2881 ttatcattta ctatgtctgc ttaacaactg tctgaatttt attattaaaa cttcaaatag
     2941 taaaactatt tcccataccc atactgatat tcctgaagta tgttgtccat tctttctcta
     3001 tagcgttttt taagttcagg gacatctgct tctgtcaatc cttttacttc aataggtttg
     3061 tcaataataa cacgtgcaat gcccggtctg ccgcccagaa tttttcttgg acaatcgggt
     3121 aatattttcc agttgtcaat ataacaaaca ggtaaaagca ttatctgatt ttctattgct
     3181 agtttaaatg ctccgttttt cattggccct aattgtggat tgcagtcagg tatcgttgcc
     3241 tccggaaaaa tagagatgct gatattttgc gaaagatcct tgcaagctct ttgatatgct
     3301 ttatgcgatg ccatccggct tgaacggttc acaccaatat tcattcgctt gaaaaaaata
     3361 ttaaagaaag gtacgcgctc taattctgcc ttacccataa aatgaaaata gtgaggaaaa
     3421 gctacgtatg tcagcacaat atcaagataa gaaatatgat tggggcagat aatataggtt
     3481 tcattctcca tcggttcata tttcctgata acttttacat ttattcctga aagaaaagca
     3541 atagtgcggg catggaattt catcagttta aatgcctgac ga
//
LOCUS       DAORQT010000162         3565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4898_length_3565_cov_4.427920, whole genome shotgun sequence.
ACCESSION   DAORQT010000162 DAORQT010000000
VERSION     DAORQT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4898_length_3565_cov_4.427920"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1868
                     /gene="ftcD"
                     /locus_tag="PK134_06165"
     CDS             186..1868
                     /gene="ftcD"
                     /locus_tag="PK134_06165"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRB38468.1"
                     /translation="MKQLIECVPNFSEGNDLTIIKQITDSIESVEGIKLLNVDPGKAT
                     NRTVVTFVGEPAAVVEAAFKGIAKAAELIDMSKHKGEHPRMGATDVCPFIPVANITMQ
                     ETAEWSKKLAARVGNKLHIPVYLYEEAQEDKKRSNLSIIRAGEYEGFFKKIKQAEWKP
                     DFGPAELPANSGATVIGARDFLIAYNVNLNTKSVKRANSVAFDIREAGRVKTDSNGKK
                     MLDDVGNEIRIPGKLKGVKAIRWYIEEYGIAQVSINITKFRETPLHLVFEESTRSAYE
                     RGYRATGSELVGLVPLQAMLDAGKYFLNKQRLSSGVSDAELIHIAVKSMGLDELSPFT
                     PEKRIIEYVMESESKPALANMRLNAFVDETASESPAPGGGSIAAYCGSLGVALATMVA
                     NLSASKKGWEERWNEFSDWADKGQKLKKQLLHLVDEDTAAFNKIMDAFKLPKTTDQEK
                     ADRKQAVAEATRYAIEVPLSVMKISLQSMELIQSMATNGNPNSVSDAGVGAMCARTAV
                     LGAGLNVRINAASYSDKAFVETVLKQAAEMESKAISNEKNILDEVNRKITGL"
     gene            1914..>3565
                     /locus_tag="PK134_06170"
     CDS             1914..>3565
                     /locus_tag="PK134_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB38469.1"
                     /translation="MNIKDRIKIFWFSFPVQLVIMHVKKSQLLLVYWLVLFACVTQNF
                     GNRFGIPYLFLDPEYMGKVSWLAFFIIGICLGVFIMAYNISSYMLNSFRFPFLACLYK
                     TFEKYCYNNAVFPVMFILTYIGGIYYFQLKNQLLPIGIVTIQVLALLLGVGFVIFSTL
                     KYFQHTNKDIYKLFGVATHDGDHDNVKFVSPIRDTKLKRQRRRGWKVETYLTFPLKLR
                     LVRSTSHYKSFMLASVFRQNHINAAVLEMIIFLLFILLGLFRDYSVFRIPAGGSILLL
                     FTMIIMIGGVFRYWLRGWAYTALVGLFILINFLSGLEVFNFKNKAYGLNYDGAQPEYS
                     IQKMEEMLSHYRLQKDYETGLVSLNNWKKKWNDAGVEKPKLVVLNVSGGGVRSALFTF
                     NAMLELDSALNGQLLKYTQFISGSSGGLIGASYYRELYLRNKGSDKIFENRDAYLKNI
                     SKDVLNATAFSFIISDLFLNFQQFSYHGKTYLKDRAYAFEEQLNENTGHVLDKQLSEY
                     YLPELKSEIPRLVICPTVVNDGRSMVISPLPSTYLLKSKNNS"
BASE COUNT         1120 a          565 c          739 g         1141 t
ORIGIN      
        1 caaagcacgc aatacgttta aaagacaaaa gaattaggct gattataccc aatgacattt
       61 gctcagggta caattaataa aatttcgttt aaaacttttt atgcttttcg ttaacgaata
      121 tgttttcaga gtaattttga tgatcttagc aaacatattt tttaaaaaga ttaaaacaaa
      181 tttaaatgaa gcagttgatt gaatgtgttc ccaatttcag tgaagggaat gatttaacta
      241 ttatcaaaca aattaccgat agcattgaat cggtagaagg aataaaattg ttgaatgttg
      301 atccgggtaa agcaacgaac cgaactgttg taacttttgt aggtgaacct gcagcagtag
      361 ttgaagcagc atttaaaggc atagcaaaag cagcagaact tattgacatg agcaaacaca
      421 aaggtgagca tccgcgaatg ggagccactg atgtttgtcc atttattcct gtagccaata
      481 ttacaatgca agaaacagca gagtggtcaa aaaaattagc tgcacgtgta ggtaataaac
      541 ttcatattcc ggtttatttg tatgaagagg cacaagaaga taaaaaacga agtaatcttt
      601 caatcatcag agctggtgaa tatgaaggct tctttaaaaa gattaaacaa gcagaatgga
      661 aacctgattt tggtcctgca gaattgcctg caaattcggg tgctaccgtt attggtgctc
      721 gagatttttt aattgcctat aatgtaaatc tgaataccaa atctgttaaa cgtgcaaact
      781 ctgttgcttt cgatattcgt gaagcaggtc gtgtgaaaac tgatagcaat ggaaaaaaaa
      841 tgttagatga tgttggtaat gaaatcagaa taccgggtaa gcttaaaggt gttaaagcga
      901 ttagatggta tattgaagaa tatggtattg cacaggtctc gattaatatt acaaagttca
      961 gagaaactcc cttgcatctt gtttttgaag agagcacacg aagtgcctat gagcgtggct
     1021 atcgagctac ggggagtgag ttggtgggct tggttccatt gcaggccatg ctcgatgcag
     1081 gaaaatattt tctgaacaag cagcgtttgt catcgggtgt cagtgatgct gagttaattc
     1141 atattgcagt aaaatcaatg gggcttgatg agttgtcgcc attcactcct gaaaaaagaa
     1201 ttattgaata tgtgatggag agtgaaagta aacctgctct ggcgaatatg cggttgaatg
     1261 cttttgttga tgaaactgcc agcgaatcac ctgctccggg tggtggatca atagctgctt
     1321 attgcggttc attaggtgtt gcacttgcaa caatggttgc caatctttct gcatctaaaa
     1381 aaggatggga agaacgatgg aatgaatttt ctgattgggc agataaaggt cagaaactta
     1441 aaaaacaatt acttcattta gttgatgaag atacagcagc attcaacaaa ataatggatg
     1501 catttaagtt accaaaaact acagatcagg agaaagcaga cagaaaacaa gctgttgctg
     1561 aagccacacg ctatgcaatt gaagtaccat tatcggtaat gaaaatttca ctacagtcaa
     1621 tggaactcat tcagtcaatg gcaacaaacg gcaatcctaa ttctgtttca gatgcaggtg
     1681 taggagcaat gtgtgcaaga actgctgttt taggcgcagg acttaatgtg cgtattaatg
     1741 ctgcatcata ttccgataaa gcttttgtgg aaacagtctt aaaacaagct gctgagatgg
     1801 aaagtaaagc aatctcgaat gaaaaaaaca ttcttgatga agtaaaccgt aaaattacag
     1861 gcctttaaaa ccaaattagc tgtgatgcgt ataatatagt tgaagatgca gtaatgaata
     1921 ttaaagacag aataaaaatt ttctggtttt cttttcctgt tcagttggta ataatgcatg
     1981 ttaagaagag ccagctgctg ttagtctatt ggttggtttt atttgcctgt gttactcaga
     2041 actttggtaa tcgctttggt attccctatc tttttcttga tccggagtac atgggtaaag
     2101 taagttggct tgctttcttt ataatcggaa tttgtcttgg agtttttatt atggcatata
     2161 acatttcaag ttatatgctc aatagtttca gatttccgtt tcttgcatgt ttgtataaaa
     2221 cttttgagaa gtactgctac aataatgctg tgtttcctgt tatgtttatt ctgacatata
     2281 ttggtggcat ctactatttt caattaaaga atcagttact tcctattggt attgtcacca
     2341 ttcaggtttt agcacttctg ctgggtgttg gttttgttat cttctcaaca ttaaaatatt
     2401 ttcagcatac caataaggat atttataaac tttttggtgt tgcaactcat gatggcgatc
     2461 atgataatgt aaaattcgtt tcaccaattc gcgatactaa acttaaacgt cagcgcagac
     2521 gcggatggaa agttgaaacc tacttaacct tccctctcaa actccgttta gttcgaagta
     2581 catcacacta taaatctttc atgcttgcat cggtgttcag acaaaaccat attaatgcag
     2641 ctgtgcttga aatgattatt ttccttctgt ttattttact cggattattt cgtgattata
     2701 gtgtgttcag aattcctgca ggaggcagca tactgttgct ttttaccatg attattatga
     2761 ttggtggcgt atttcgttac tggcttcgtg gttgggcata tactgcattg gttggcttgt
     2821 ttattctgat aaacttttta tcaggccttg aagtttttaa tttcaagaac aaagcttatg
     2881 gtcttaatta tgatggtgca caacctgagt attctattca gaaaatggag gaaatgttaa
     2941 gtcactaccg tttacagaaa gattatgaaa caggtttggt aagccttaat aactggaaga
     3001 agaaatggaa tgatgccgga gttgaaaaac cgaaacttgt tgtgttgaat gtaagtggtg
     3061 gtggtgttcg ttctgcctta ttcaccttta atgcaatgct tgagttagat agtgcattaa
     3121 acgggcagct acttaaatac acacaattta tcagtggctc ttcaggtgga ttgataggag
     3181 cttcttatta ccgtgaattg tatctgcgaa ataaaggctc agataaaata tttgaaaatc
     3241 gagatgccta tctgaaaaat atttctaaag atgtgttgaa tgctacagca tttagtttta
     3301 tcatcagtga tttgtttctc aattttcaac aattcagcta ccacggaaag acctatctta
     3361 aagaccgtgc ttatgcattt gaagagcagt tgaatgaaaa taccgggcat gtactcgata
     3421 agcaattgag tgaatattat ttgcctgaat taaaatctga aataccaaga ttagttattt
     3481 gccctactgt ggtgaacgat ggtcgttcca tggttatttc tcctctgcca tcaacctatc
     3541 ttctgaaaag taaaaacaac agtaa
//
LOCUS       DAORQT010000163         3545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4934_length_3545_cov_5.048711, whole genome shotgun sequence.
ACCESSION   DAORQT010000163 DAORQT010000000
VERSION     DAORQT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4934_length_3545_cov_5.048711"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK134_06175"
     CDS             complement(<1..342)
                     /locus_tag="PK134_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38470.1"
                     /translation="MATSFFNNWDDPTFAKRNELVFDGRNKSYGAYLIRQLYSKRINV
                     AFIIAAAGLILFISMPLILELMGDKATENVAQQEVEVTLSEPPPVDPAEPPPPPPPPP
                     PPGFKTKKFKPP"
     gene            complement(345..956)
                     /locus_tag="PK134_06180"
     CDS             complement(345..956)
                     /locus_tag="PK134_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRB38471.1"
                     /translation="MAEVNTDQGGGKEKGGKHSKVRAKKASTHIDMTPMVDLAFLLLT
                     FFMLTTTFSKPKTVEINMPLKDGEPTKVNNAITVLLSDKDRVFWYFGEFKPGETQLNK
                     SDFSDNGIRKILLDKNAVAHAEVKKLEDELKSRQIADSTYKRLAVEAKSQKSALMVLI
                     KTDDKAKYRNVIDILDELSIASVGKYAVVDMMKDEYDLIQKMN"
     assembly_gap    1121..1151
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1152..1566)
                     /locus_tag="PK134_06185"
     CDS             complement(<1152..1566)
                     /locus_tag="PK134_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRB38472.1"
                     /translation="MVDLAFLLVTFFMLTTKFRPDEPVIVDTPASVSTKILPENTLQV
                     TIDSAGRVFFNIEGQNVRRNLMTRISEKYKFQLTEADLKKFSLMSTFGMPAEKIKAYL
                     EAPDSERKKMDKATGGIPIDTLNNQLADWIVFGWTA"
     gene            complement(1638..2444)
                     /locus_tag="PK134_06190"
     CDS             complement(1638..2444)
                     /locus_tag="PK134_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRB38473.1"
                     /translation="MSSSNNSGGGFKSIFAAIVIPAAILVAILIYAYILGNPANFMEN
                     DPNNHPQNYLGIIHKGGVIVPILISLLLIVITISIERMMTIGASKGKSSVDSFVGKIR
                     SLLSNGDLNTAIAECDKQKGSIANVVKAGIESYRKMQGETSLDKERKTAAIQKELDEA
                     TTLELPMLSKNLVIISTVASVATLMGLLGTVIGMIRAFAALAQAGAPDAIALATGISE
                     ALINTALGVGTSALAIIMYNYFTNKIDSLTYGIDEASYSIVQSFTANKLA"
     gene            complement(2602..2784)
                     /locus_tag="PK134_06195"
     CDS             complement(2602..2784)
                     /locus_tag="PK134_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRB38474.1"
                     /translation="MNESKRLVKGEKKLFGVCSGIANYFEADPTLIRLLFVIAVFGFG
                     TGVLLYIILAIIMPEK"
     assembly_gap    2809..2838
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2962..3396
                     /locus_tag="PK134_06200"
     CDS             2962..3396
                     /locus_tag="PK134_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRB38475.1"
                     /translation="MKAITFILAVVMLTSSSCLNLKMPQVTGVSKLRLSDITKDTRIQ
                     FDVGIRNPNTFGVTLKSMKAELFLADSSIAGIGVDRKTRLAANQQVDMPFSVQPKLTA
                     LPQLGILGITQLFKKDNKKISLRGELKVRKFIFTKKVKFSIP"
BASE COUNT          998 a          663 c          632 g         1191 t
ORIGIN      
        1 cggtggttta aacttttttg ttttaaaacc gggggggggt ggggggggag gtggcggtgg
       61 tggctctgca ggatcaaccg gtggtggctc agataatgta acttcaactt cttgttgtgc
      121 tacattttct gtggctttat cacccatcag ctccagaatt aacggcatac tgataaataa
      181 aattagtcct gcggctgcaa taataaatgc tacattaatg cgcttgctat aaagttggcg
      241 tattaaatat gcgccatagc ttttatttct tccatcaaat accaactcat ttcgtttggc
      301 aaatgttgga tcgtcccagt tattaaaaaa acttgttgcc atgattagtt cattttttga
      361 attaaatcat attcatcctt catcatatct actactgcat atttacccac actggcaatg
      421 ctcagttcgt caagtatgtc tatcacattt ctgtattttg ctttatcgtc tgttttaatc
      481 agaaccatca atgcgctttt ttgtgatttg gcttctactg ccaatcgctt atatgtagag
      541 tctgctatct gacgactttt caattcatct tcaagctttt ttacttctgc atgtgcaaca
      601 gcatttttgt ctaataatat tttgcgaata ccattatcag aaaaatcaga tttgttcagt
      661 tgtgtttctc cgggtttaaa ttcaccgaag taccaaaaca ctctgtcttt atcactcaat
      721 agtacagtta ttgcattatt aactttcgtt ggctcaccat cttttaacgg catattaatt
      781 tcaaccgttt ttggtttgct gaaagtggta gtaagcataa aaaaggtgag tagcagaaac
      841 gccagatcca ccataggtgt catgtcaatg tgagtggagg ctttcttggc gcgaacctta
      901 ctatgtttcc cgcctttctc tttaccacca ccctggtcgg tatttacttc agccattttt
      961 tcttattgtt ctactggtgt tgcttgtaaa tttgttatca aattaaaacg gttgattttg
     1021 ttttgctgaa aaatttcaat cacacgttta atgtaagtat aatcaaccct gccgtcacct
     1081 tttatggcaa accgcagttc attgcctgct tgtttacggg nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nctgctgtcc agccaaaaac tatccagtcg gcaagttgat tattgagagt
     1201 atctatcgga attccgcctg tggccttatc catctttttg cgctcggaat cgggtgcttc
     1261 aagataggct tttatttttt cggcaggcat tccaaatgtt gacatcaagg agaatttttt
     1321 cagatccgct tctgtaagct ggaacttgta tttctcactg attcttgtca tcaggtttct
     1381 gcgcacattt tgtccttcaa tattaaaaaa cacacggcca gctgaatcaa ttgttacctg
     1441 caatgtattt tcaggtaaaa tttttgttga aacagatgcc ggtgtgtcaa caatgaccgg
     1501 ttcgtccgga cggaatttgg tagttaacat aaagaatgta accagcaaaa acgccagatc
     1561 caccatgggt gtcatgtcta acgatggact ctttttgggt attttaattt ttggcattat
     1621 ctaatgctga taattaatta cgctaattta tttgctgtaa acgattgaac aatgctatag
     1681 cttgcctcat ctattccata agttaacgaa tcaatcttat tagtgaaata gttgtacatg
     1741 atgattgcta aagctgacgt tcctactcca agtgccgtat taatcaaggc ctcagaaata
     1801 ccggttgcaa gcgctatggc atcaggtgca ccggcttgtg caagagcagc aaacgccctg
     1861 atcataccga taaccgttcc taatagtccc atcaatgtag ctacagatgc tactgtagag
     1921 atgataacca gattttttga aagcattggt aactctaaag ttgtggcttc atctaactct
     1981 ttctggatag ctgctgtttt gcgctcttta tccaaagaag tttcgccctg cattttgcga
     2041 taagactcaa tgcctgcttt tactacattg gcaatagaac ctttttgttt gtcgcattct
     2101 gcaatggctg tatttaaatc gccattgctt agcaaacttc ttattttgcc tacgaatgag
     2161 tcaacagatg atttgccttt tgaagcacca atagtcatca tacgctcaat agaaatggtg
     2221 attacaatta ataataagct aatcagaata ggcacaatta cacctccttt atgaataatt
     2281 cccagataat tctgtggatg attattcggg tcgttttcca taaagtttgc cggattacct
     2341 aatatataag cataaattag gatggcaacc aagattgctg ccggaattac tattgctgca
     2401 aagatggatt tgaaaccacc gcctgaattg ttagatgaac tcatgttcgt ttttaattta
     2461 agttttaaat ttgattatct atgtttaatt atttgtcaaa atggagggca agtataatga
     2521 aaacttgaca atttgccgct ttatttttga attcatgaat attacggttg ggtgtattgt
     2581 tttttcagaa atcataaaat attacttttc gggcataatt atcgcaagta tgatgtataa
     2641 caacacacct gttccaaatc caaaaactgc aataacaaaa agcaagcgaa ttaatgtagg
     2701 gtcggcttca aaataattgg caatgccact gcatacacca aatagttttt tctctccttt
     2761 aactaatcgt ttagattcgt tcatgctgtt ggttcagata gataatggnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnntt attctcgcgg tcaaaaataa ataaattgtt taatttgcaa
     2881 gttcatttta atcatcttta taaataactc catatttttt acattatttt tatcttttgg
     2941 cgtttatttt aaaatttctg tatgaaagca attacattta tcttagcagt tgtaatgtta
     3001 acttcaagta gctgtcttaa cctcaagatg cctcaggtta ccggagtaag taaattaaga
     3061 ttaagcgata tcactaaaga cacacgaata cagtttgatg taggcatccg taatcccaat
     3121 acatttgggg taactttaaa atcaatgaaa gcagaattgt ttcttgccga cagttccata
     3181 gcaggaattg gtgttgacag aaagacaagg ttagcagcca atcagcaagt tgatatgcct
     3241 ttttcagtgc aacctaaact tacagcattg ccacaattgg gtattctggg aataacacaa
     3301 ctttttaaga aagacaataa aaaaatcagc cttagaggag agttaaaggt cagaaagttt
     3361 atttttacca aaaaggttaa gttttccatt ccatagtatc actccaccct tgatctcagt
     3421 aatcccaatt gaccagtttg taattaatca tgcatcgcat gaagtattgc ttgacacacg
     3481 tagtcctgca gagtatgagc atgcacattt tccgggagcg gtttcatttc cattaatgac
     3541 taatg
//
LOCUS       DAORQT010000164         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_4992_length_3516_cov_6.549552, whole genome shotgun sequence.
ACCESSION   DAORQT010000164 DAORQT010000000
VERSION     DAORQT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4992_length_3516_cov_6.549552"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PK134_06205"
     CDS             complement(<1..375)
                     /locus_tag="PK134_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRB38476.1"
                     /translation="MLNIVLFGPPGAGKGTQSEKLIQQYGLIHLSTGDLLRNEIAGQT
                     KLGLEAKKLMDQGVLVPDEVVIGMIESKLDANPTAKGFIFDGFPRTTAQAAALDNLLK
                     QKNTSIKMMLALEVTEAELSKRL"
     gene            complement(435..971)
                     /gene="hpt"
                     /locus_tag="PK134_06210"
     CDS             complement(435..971)
                     /gene="hpt"
                     /locus_tag="PK134_06210"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRB38477.1"
                     /translation="MSEVTILDKTFTIMIPRKQIETRILEIARDLNAEYKDKNPVFIG
                     VLNGAFLFMADLFRNIETPCEISFIRVSSYSGTESTGQMKNVVGLSGNINNRHVIIVE
                     DIVDTGETVSYLMQEIAKHQPASVKLATLLFKPQALKKKVTVDYAGFEIPPAFVVGYG
                     LDYDGYGRNLQDIYVLKS"
     gene            1098..2228
                     /locus_tag="PK134_06215"
     CDS             1098..2228
                     /locus_tag="PK134_06215"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRB38478.1"
                     /translation="MNAFNPNIVIGVLGGGQLGRMLLQKAADYNLSIHILDPDEAAPC
                     RYMCKTFEHGDFRDYQAVMAFGNDCDIITIEIEHVNIEALYELEKLGKKVYPQPHILE
                     MVQDKGLQKEFYQKNNLPTAPFYLVEKPEQIKPEFPFVQKLRKGGYDGKGVLVVKDAD
                     GMSQLFQAPSVIENKVVIEAEIAVITARNNSGEIKSFPVVEMQFNAEANLVELLFSPS
                     KTSAVIQQKAVSIAEDLISKLNMVGLLAVEMFVTKDGQVLINEIAPRPHNSGHHTIEA
                     NHTSQFEQHLRAILNLPLGNSSTILPAAMINLLGDKDSNGRAIYSGVQDALNAGDVHI
                     HLYGKKITKPYRKMGHVTVTAATVDEAILNAQKIQSQLKVIS"
     gene            complement(2221..3039)
                     /locus_tag="PK134_06220"
     CDS             complement(2221..3039)
                     /locus_tag="PK134_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38479.1"
                     /translation="MENNKMNKLFDAARNADPVVSLDESLEQFNKEVEGKETTLGYMK
                     WLKPVIAVATIAVVGGGIYFLVQNQGQQKTQPTQENTEQTTQPALQATETQPDAAIEQ
                     PQATEENLTETPSVKTEITNPAPLNNKVTHAASANDAYIVETQTVNINNDNGAFKISI
                     SGDDVERIVLNGKEVEKSDWSKYQDEVAEAMAAKSSMGINKENAANKKFVDYMFTTLK
                     ENGLIKNNLAVIKLSNDMLLIEGQEADSAIHQQLLKEYKKITGKDIGLRTLYFN"
     gene            complement(3061..>3516)
                     /locus_tag="PK134_06225"
     CDS             complement(3061..>3516)
                     /locus_tag="PK134_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB38480.1"
                     /translation="HEKLVRFVQSMVYNREDVKDIVAETMLVAYEKFETIRHKDALLS
                     YLFTVSSRLVYKLQDKKKQIYTAFDPEQAEQVSDDHAGADSKVQLKELYNALDQLPLK
                     QKEAIVLFEISGLSLAEIQQIQGDSLSAVKSRIARARESLKKILEEEIV"
BASE COUNT         1015 a          649 c          646 g         1206 t
ORIGIN      
        1 tagtcgcttc gataattcag cttccgttac ttctaatgca agcatcattt taattgaagt
       61 gtttttttgc tttaataaat tatcgagtgc tgctgcctgt gccgttgtgc gagggaagcc
      121 atcaaaaata aatcctttgg cagttgggtt ggcatcaagt tttgattcta tcattcctat
      181 aactacttca tcgggaacta atacaccctg gtccatcagt ttttttgctt caagacccaa
      241 tttagtttgt ccggcaattt cgtttcgcaa caaatcgcct gttgataaat gtatgaggcc
      301 atattgctga ataagttttt ctgactgtgt tcctttgcct gcacccggag gaccaaataa
      361 tacaatgttg agcatgataa aaaataaatt aaagagggtt tggtgatgaa tttctcattt
      421 cgttttattc gatattatga ttttagaaca taaatatcct gaagatttcg tccgtagcca
      481 tcatagtcta agccatagcc tacaacaaat gcaggtggaa tttcaaaccc tgcatagtca
      541 actgtaactt ttttctttaa tgcctgtggt ttaaaaagta atgttgccaa tttaaccgat
      601 gccggttgat gttttgctat ttcctgcatc agatatgaaa ctgtctcgcc tgtatcaact
      661 atatcttcaa caataataac atgcctgttg tttatattcc ctgaaagacc aactacattt
      721 ttcatctgcc cggtagattc tgtgccgcta tatgatgata ctctgataaa agaaatttcg
      781 catggagtct caatgttacg gaatagatcg gccataaaaa gaaaggcacc gttaagcaca
      841 ccaataaata cagggttttt gtctttgtat tcagcattca ggtcgcgtgc aatttccaga
      901 attctggttt cgatttgctt tcgcggaatc atgattgtga atgttttatc aagtatggta
      961 acttcgctca ttgccacttt tttgggtgcg gctaaagtaa aaataaattc cctatgactg
     1021 acggggtttc tattcttagc ttaatttgtt ttcattgcca caggttcttt atttttgttt
     1081 aataaatagc gttaattatg aatgcgttca atcccaatat agttataggt gttttaggtg
     1141 gtggccagct tggcagaatg ttattgcaaa aagcggcaga ttacaatctt tctattcata
     1201 tacttgatcc tgatgaggct gcaccttgcc gctatatgtg taaaacattt gagcatggcg
     1261 attttcgcga ttatcaggct gtcatggcct ttggtaatga ttgtgatatt attaccattg
     1321 aaattgaaca tgtaaacata gaagcattat acgaactgga aaagttggga aaaaaggtgt
     1381 atcctcaacc acatattctt gaaatggttc aggataaagg acttcagaaa gaattttatc
     1441 agaagaataa tttacctaca gcgccatttt atttagtaga aaaaccggaa cagataaagc
     1501 cggagtttcc atttgttcaa aaactgcgca aaggtggtta tgatggtaaa ggtgttttgg
     1561 ttgttaaaga tgcagatggc atgagtcagt tgtttcaggc accaagtgta attgagaata
     1621 aagtagttat tgaagcagaa attgctgtaa ttacagcaag aaataattcg ggagaaataa
     1681 aatcatttcc agtagttgaa atgcagttta atgcagaagc caatttggtt gaattacttt
     1741 tttctccatc aaaaacttca gccgtcatac aacagaaagc agtttcgatt gcagaagatt
     1801 taatcagtaa actgaacatg gttgggctgc ttgctgtaga aatgtttgtt acaaaagatg
     1861 gacaagtgct gattaatgaa attgcaccac gaccacacaa tagcggacat cataccatag
     1921 aagcaaatca tacttcgcaa ttcgagcaac atttaagagc aattcttaat ttaccacttg
     1981 gtaactcatc aactattttg cctgcagcaa tgattaacct gttaggtgat aaagattcta
     2041 atggtagggc gatttattca ggtgtgcaag atgcattaaa tgcaggtgat gtgcacattc
     2101 atttatatgg taagaaaatc actaaacctt atcgtaaaat gggacatgta actgtaacgg
     2161 cagctactgt tgacgaggca attttaaatg cccaaaaaat tcaatcgcag ttaaaagtaa
     2221 tcagttgaag taaagtgtac gtaagcctat gtccttgccg gttatttttt tgtactcttt
     2281 caataattgt tgatgaatgg cagagtctgc ctcttgccct tctatcagca gcatgtcgtt
     2341 actgagttta attacagcaa gattattttt tatgaggcca ttttctttta aggtcgtaaa
     2401 catataatca acaaactttt tgtttgcagc attttctttg tttattccca ttgagctttt
     2461 tgccgccata gcttccgcta cttcatcctg atattttgac cagtcggatt tctctacctc
     2521 tttaccattt aagacaattc gttcaacatc atcaccgcta attgaaattt taaatgcacc
     2581 attatcatta ttaatattca cagtttgtgt ttcaacaatg tatgcatcat ttgcactggc
     2641 agcatgtgta actttgttat ttaatggggc aggattggtg atttctgttt taactgaagg
     2701 tgtttctgtt aagttttctt cagttgcctg aggttgttca atagcagcat caggctgtgt
     2761 ttctgttgcc tgtaatgcag gttgagttgt ttgctctgta ttttcctgag ttggctgtgt
     2821 tttttgttga ccttgattct gaactaaaaa ataaattcct cctccaacaa cagcaattgt
     2881 tgcaacagca atgacaggct ttagccattt catgtaaccc aaagtggttt cttttccttc
     2941 aacttctttg ttaaattgtt ctaaactttc atccagcgag actacaggat ctgcatttct
     3001 tgctgcatca aacaatttat tcatcttatt attttccatg gtgaaaaaag taatttacaa
     3061 ttatacaatt tcttcttcta atattttttt aagtgactcg cgtgcccggg caattctgct
     3121 tttcacagca gaaagactat ctccctgaat ctgctgtatt tctgctaacg atagccctga
     3181 gatttcaaac aacactatgg cttctttctg ttttagcggc aattgatcta aggcattata
     3241 caattctttt aactgaactt tgctatcggc accggcatga tcatcactca cttgttcagc
     3301 ttgttccggg tcaaaggctg tatagatttg ctttttttta tcctgaagct tataaaccaa
     3361 cctgcttgaa acggtaaaca aataactcaa taatgcatct ttatggcgaa tggtttcaaa
     3421 cttttcataa gctaccaaca ttgtctctgc aacaatatct tttacatcct cccggttata
     3481 aaccatagac tgcacaaagc gaaccagctt ttcatg
//
LOCUS       DAORQT010000165         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5015_length_3509_cov_12.078170, whole genome shotgun sequence.
ACCESSION   DAORQT010000165 DAORQT010000000
VERSION     DAORQT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_3509_cov_12.078170"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK134_06230"
     CDS             <1..467
                     /locus_tag="PK134_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38481.1"
                     /translation="ERNMEFKFDNILLPDSNVNEPLSHGFIHYRIKPKTSLVLNDSIQ
                     NTAAIYFDFNDPVITNTATTHVVLPTGIASYQSAENIQLFPNPAQSELNIQLTTAVEK
                     IQVIDVLGKILFEKKTNNQQNLKINISQFQNGVYFVKAYSTQGVVVKKFVKR"
     gene            complement(661..1074)
                     /locus_tag="PK134_06235"
     CDS             complement(661..1074)
                     /locus_tag="PK134_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38482.1"
                     /translation="MKHRLTIQAEDTILDFQKKFNRIYPFLKIEFFEKAHIAKQGSHK
                     KEMIASNMLFRGLIGRGKSHIINFNDDFSISDFEMLLNDQCRLYAQVFRKSGKIWLET
                     SATDYWTLEQQNEEGKSLAKHFKTESENINDIDPY"
     gene            complement(1153..1974)
                     /locus_tag="PK134_06240"
     CDS             complement(1153..1974)
                     /locus_tag="PK134_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB38483.1"
                     /translation="MKKIVVTTDFSECSENALIYSLSLCKEVNASLYLLHVFHIPVPV
                     TDMPYISVSSEEEEKNIELRLKELITRIRHKTGYEGMIHTITDFNFTVDGILNVVNDT
                     NADLLVMGQRGKTTLADRLFGSTATAIINLATCPLLLIPEGCSFSKIKKIAIACDKKT
                     SDNKNKVFHVVEMNAALKSEMLLFNVVQDEAEKDRLKGEDFTEMAGKEVKLFFPVHHD
                     IHEGILQFVNDNDADWLCMFPHRHNIFEQWFTRLNSGNISQQVKIPLLLINELVQ"
     gene            complement(2257..>2976)
                     /locus_tag="PK134_06245"
     CDS             complement(2257..>2976)
                     /locus_tag="PK134_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB38484.1"
                     /translation="ITTGLSVPPEYFFKVMSINIMGCRPMKDVFRNNLNFLSPVNFKA
                     ETQRALIVDTRNELEFSKAFIKGSVNIGLDGSFATWAGILLDPDHPVLLVCDSGKEKE
                     AIGALAQIGIENVRGILHGGIKSWKDAGQPVDNVENIDAEDFYFLFENEGYILVDLRS
                     HQNFNAQHIKRSLSIPLDELPHRLNEFSPDTKYLLVCKGGYHSMIAASILKRNGINQV
                     VNISGGFDAVSAFQPQYVESI"
     assembly_gap    2977..2990
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2991..>3509)
                     /locus_tag="PK134_06250"
     CDS             complement(<2991..>3509)
                     /locus_tag="PK134_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB38485.1"
                     /translation="LADKQLILKYIFETHIHADFVSGHLELADKTGATIVYGPQVRVA
                     YPSYSARHNESFNLEGCSIKALHTPGHTMESVCYLLNNNVGQPIAIFTGDTLFVGDVG
                     RPDLMSGNKNAVELSEYLYHSINDIIKTLPDELVVYPGHGAGSFCGKNPGKEKFSTIG
                     EQKKRNYALKDI"
BASE COUNT         1139 a          698 c          510 g         1148 t
ORIGIN      
        1 atgagcgcaa catggaattt aagtttgata atattctatt gcccgatagt aatgttaacg
       61 agccgttaag ccatggcttt attcattaca gaataaaacc caaaacaagt ttggtattaa
      121 atgatagcat acaaaacact gctgccattt attttgattt taatgaccct gtaataacca
      181 acacagcaac aacgcatgtt gtgctaccaa caggaatagc gtcatatcaa agtgcagaaa
      241 acatacaatt gttcccaaat cctgctcaat cagaattaaa tattcaactc acaacagcag
      301 ttgaaaaaat acaagtaata gacgtacttg gaaaaatttt gtttgaaaag aaaaccaaca
      361 accaacaaaa tttaaagata aacatcagtc aatttcaaaa cggagtttat tttgtgaaag
      421 catacagcac acaaggtgtg gtagtcaaga agtttgttaa gaggtagaaa atttttgtat
      481 tgttgatcag ccacgactca gccgcggctg aagagattta aggatttgaa gaatctcctg
      541 aattttattt ccaataattc agtcaagtca tattctcaaa taatatgaga ttgttgtatt
      601 cacatttgtt attaagctat tacttagtaa aatttctacc tatgtagaaa gaagaagtaa
      661 ttagtaaggg tcaatatcat ttatgttttc tgactctgtt ttaaagtgct tggctaagct
      721 tttgccttct tcgttttgtt gctccaatgt ccaataatct gttgctgaag tttcaagcca
      781 aatctttcct gatttacgaa atacttgtgc atataaccga cactgatcat tcagtaacat
      841 ttcaaaatcg ctaatactaa aatcgtcatt gaagtttatt atatgtgatt ttccgcgtcc
      901 tatcaatccg cgaaacaaca tgtttgaagc aatcatttcc tttttatgac ttccctgctt
      961 ggctatatgt gctttttcaa aaaattcaat tttaagaaac ggataaatcc tgttgaattt
     1021 tttttgaaaa tccaaaatag tatcctctgc ctgaatagtc aacctatgct tcataattta
     1081 aattgaatcg taaccgcttc tttataagat ttaaaatcat cgtattcata aaagtacttt
     1141 ttagcaatat ttttactgta ccaactcgtt aattaataat aaaggaattt ttacttgctg
     1201 cgaaatgtta cctgaattaa gtctggtaaa ccactgctcg aaaatgttat gacgatgtgg
     1261 aaacatacac aaccaatcag catcattatc atttacaaat tgcaaaattc cttcatgtat
     1321 gtcgtgatgt acagggaaaa ataatttcac ctcttttcca gccatttcag taaaatcttc
     1381 tcctttaagt ctatcctttt ccgcttcatc ctgaacaaca ttaaacagta gcatttccga
     1441 cttcaatgca gcattcattt caacaacatg aaatactttg tttttattgt cactcgtttt
     1501 tttatcgcag gcaattgcaa tctttttaat tttactgaaa ctgcatcctt caggtataag
     1561 taataaaggg caagttgcaa gattaataat tgctgttgca gtacttccga acagtctgtc
     1621 cgccaatgtt gttttacctc gttgtcccat caccagcaag tctgcatttg tatcattaac
     1681 aacattcaga ataccatcaa ccgtaaaatt aaaatcggtt atggtgtgta tcataccctc
     1741 ataacctgtt ttatgtctta tgcgggtaat caattctttc aaacgtaatt caatattctt
     1801 ttcctcctcc tcagacgaaa cagaaatata tggcatgtcg gtcactggaa ctggtatatg
     1861 aaaaacgtga agtaaataca atgatgcatt cacctctttg cacagtgaca aggaataaat
     1921 caaagcattt tcagaacact ccgaaaaatc agtagtaaca acaatttttt tcatacttat
     1981 gctttttacg ataatcctat taagcaaaaa ccggtagccg aagcataccg gttttgcaac
     2041 cctaacacta aatcaaatct ttttatttta aaccggaggg tatttccaaa ccggaatttt
     2101 attgctttcc ttttaattaa cgatgtaaaa gtatttcctt tattataacc taaaaatgac
     2161 tttaagcaac ccataaagcg acttttgtca taaatttaag gtcatgcctg ttttatacct
     2221 aatactgttt aaattgtcag taaccttctg ttcacactat atcgattcta catactgtgg
     2281 ttgaaatgca cttactgcat caaaccctcc tgaaatattt accacctgat ttatcccatt
     2341 gcgtttaaga attgatgctg ctatcatact atggtaacca cccttacaca ctaacaaata
     2401 tttagtgtcc gggctaaact cattcaaacg gtgtggcaat tcatctaatg gaatactcag
     2461 acttcttttt atatgttgtg cattaaaatt ctgatggctt cttaaatcaa ccagaatgta
     2521 accttcattt tcaaacaaaa aataaaaatc ttctgcatca atattttcaa cattgtccac
     2581 cggttgccct gcatctttcc atgactttat tcctccatgt aaaatacctc tcacattttc
     2641 tattcctatc tgtgctaaag caccaatggc ttctttttcc ttaccactgt cgcagaccaa
     2701 taaaaccgga tggtcgggat cgagcaaaat ccctgcccat gttgcaaacg aaccatcaag
     2761 accaatgttt acagagcctt taataaatgc tttcgaaaac tccaattcat ttcttgtgtc
     2821 cactatcaat gcacgttgtg tttctgcttt aaaatttacc ggtgacagaa aattcagatt
     2881 gttgcgaaac acatctttca tagggcggca acccataata ttaatgctca taaccttaaa
     2941 aaaatattca ggtggcacac taaggcctgt agttatnnnn nnnnnnnnnn aaatatcctt
     3001 taaagcatag tttctcttct tttgttcgcc aatggtagaa aacttttctt tacccggatt
     3061 ttttccacag aacgaaccgg caccatgtcc cggataaaca acaagttcat caggtaatgt
     3121 tttaatgatg tcgttaatag agtgatataa atactctgac aattctactg catttttatt
     3181 accgctcatt aaatcaggac gacctacatc acctacaaat aaagtatcac cggtgaaaat
     3241 tgcaataggc tgcccaacat tattattcaa taaataacaa accgattcca tagtatgtcc
     3301 cggtgtatgt aaagctttaa tactacatcc ttcaagatta aaactttcat tatgtcttgc
     3361 actatatgaa ggataagcca cacgcacctg aggtccataa acaatggttg ctccggtttt
     3421 atcggcaagc tccaggtgac ctgaaacaaa atcggcatgt atgtgcgttt caaaaatata
     3481 ttttaaaata agctgcttat ctgcaaggt
//
LOCUS       DAORQT010000166         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5089_length_3481_cov_3.699942, whole genome shotgun sequence.
ACCESSION   DAORQT010000166 DAORQT010000000
VERSION     DAORQT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5089_length_3481_cov_3.699942"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK134_06255"
     CDS             complement(<1..190)
                     /locus_tag="PK134_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38486.1"
                     /translation="MQTFEGIVVKTTGNIITVQTGNERILCRIKGNFRIKDINSTNPV
                     AVGDYVKYYFEGDDALITD"
     gene            complement(195..899)
                     /locus_tag="PK134_06260"
     CDS             complement(195..899)
                     /locus_tag="PK134_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRB38487.1"
                     /translation="MKKLKHYSHLIFDLDHTLWDTNVNAAQSLSEMYHQYSLEQYGII
                     SEQDFIQKYVDINNRMWTEYSLGRIKKSYLRAGRFELTLKYFGIVNPELVEKLSDFFV
                     QNTPGKKELIPFAKDLLNHVHGKYQLAIITNGFSEAQHTKLRSSSIDHFFSKIIISEE
                     IGYHKPDPRIFLQTAELLQTPIKDCLMIGDNIETDIAGAIASGMDCAYLNPAGIKHNY
                     EVTFETHCLSELIKWL"
     gene            complement(896..1477)
                     /locus_tag="PK134_06265"
     CDS             complement(896..1477)
                     /locus_tag="PK134_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38488.1"
                     /translation="MGEFVKILMVILISSVKFVVGPAFAYYDEKYDFTIFEAIVYPII
                     GGMLGVFVFSFFSDQVANVWHWIKNRIKKTVSRKQPFTEPTIDSDGKVVVNYTYVDAK
                     EKPKNKIFTKRNRRIVTIWKKYGLFGIALLTPDIISIPIGTIIASRLVPDKKKVFLYM
                     LISISVWSIIMVSSFELYHAYSIKALQQKVLEP"
     gene            complement(1529..2176)
                     /gene="upp"
                     /locus_tag="PK134_06270"
     CDS             complement(1529..2176)
                     /gene="upp"
                     /locus_tag="PK134_06270"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRB38489.1"
                     /translation="MVNILSTGNSLLHHFVAELRDVEIQNDRMRFRRNLERIGEIAAY
                     EISKTMIYENTDVTTPLGIATVPLMQSQPVLGTILRAGLPLHQGLLNYFDQADNAFIS
                     AYRRHHKDGSFDIHLQYLSSPPIAGRVLILSDPMLATGQSLVETYKAMLESGQPAHTH
                     VVAAIASRQGIEYVKANLPDDLTIWVAAVDEELTAQSYIVPGLGDAGDLAFGEKK"
     gene            complement(2179..3090)
                     /locus_tag="PK134_06275"
     CDS             complement(2179..3090)
                     /locus_tag="PK134_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38490.1"
                     /translation="MEVKFLEHIEIDKAKWDQSINQALNKLPYAFSWYLDAVSPNWHA
                     LVSDDYNFVFPLTWRKKLCFNYLCQPLFTQQLGIFSSQPIDFAISNAFLNAIPSKFKL
                     IEININSFNPAAGNKFIAAKRLNFELELNRPYEEIRKFYSDNQKRNINKAKRNELSLN
                     QPDISEVISLFRHDRGMKIERMKDESYELLRILYSTLKQKGLCYLRGVSDGNQTICGG
                     LFIQTDYRIIFLFSGNSVAGKSSGAMSLLIDSVIQENANKNLVLDFEGSNDFGLARYY
                     SSFGSIEHVYLHLKKNTLPFPVRLFKR"
     assembly_gap    3204..3236
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1112 a          630 c          556 g         1150 t
ORIGIN      
        1 gatctgtaat caatgcatca tcgccttcaa aataatattt tacataatca ccaacggcta
       61 cgggattggt gctgttaatg tctttaatcc taaaattacc ttttatccgg caaagaatcc
      121 tttcgtttcc tgtttgaacc gttataatat tacctgttgt ttttacgact atgccttcaa
      181 aagtttgcat aacattaaag ccatttaatc agttcactca gacaatgcgt ttcaaaagtt
      241 acttcgtaat tgtgtttgat tccggccgga ttcagataag cacaatccat acctgaggca
      301 atagcaccgg caatatcggt ttctatgtta tccccaatca tcaagcaatc ctttattgga
      361 gtctgaagta attctgctgt ctgcaaaaat attctagggt ctggcttgtg gtagccaatt
      421 tcttcggaaa taattatctt actaaaaaaa tgatctattg atgatgaacg caactttgta
      481 tgctgagctt ctgaaaatcc attagtaata atagctaact gatatttgcc atgtacatga
      541 tttaataaat ctttagcaaa tggaattaac tcctttttgc cgggtgtatt ttgaacaaaa
      601 aaatcagaaa gtttttcaac taactccggg tttacgattc caaagtattt taaagtgagt
      661 tcaaatcgtc ctgcacgcag ataagatttt ttaatccgac caagagaata ttcagtccac
      721 attctgttgt taatgtcaac atacttttga ataaaatctt gctcgctgat tatgccatac
      781 tgttctaaac tatattgatg atacatttca gacagtgatt gagcagcatt aacatttgta
      841 tcccacaaag tatggtcgag gtcgaaaata agatgcgaat agtgctttaa ctttttcatg
      901 gctcaagaac tttttgttgt aatgctttta tagaataggc atgatatagt tcaaaagaag
      961 agaccatgat aattgaccag actgaaatag aaatcagcat atataaaaaa actttctttt
     1021 tatcaggaac taatcttgat gcaatgattg taccaattgg tattgaaata atatcagggg
     1081 taagtaatgc aattccaaat aaaccatatt ttttccagat ggttacaatt cttctgtttc
     1141 gtttggtaaa aattttattt ttaggctttt ctttagcatc tacatacgtg taattgacaa
     1201 caactttacc atcgctatct attgttggtt cagtgaaagg ttgtttgcgt gaaacagttt
     1261 tttttattct attttttatc caatgccaaa catttgcaac ctgatcacta aaaaagctaa
     1321 atacaaaaac accaagcatc cccccgataa ttggataaac tattgcttcg aaaattgtga
     1381 agtcatactt ttcgtcatag taagcaaagg caggtccaac aacaaatttt acactgctga
     1441 ttaatataac catcagaatt tttacaaact cacccatgtt ttaatattct gattaatcaa
     1501 cgaaagatag taaaataatc tgtttgaact actttttttc gccaaatgct aaatcaccgg
     1561 catcacccaa gccgggaaca atgtaagact gtgcagtgag ctcctcgtca actgcggcaa
     1621 cccaaatggt taaatcatcg ggtaaatttg ctttaacata ttcaatccct tgtctgcttg
     1681 caatagctgc taccacatgt gtatgtgcag gttgtcctga ttcaagcatt gccttataag
     1741 tttcaactaa tgactgaccg gtggcaagca taggatcact taaaattaga actcgtccgg
     1801 caattggcgg tgatgataga tactgaagat gaatatcgaa actaccatct ttatgatgac
     1861 gtctgtaagc tgaaataaat gcattatctg cctgatcgaa ataattcaac agtccttgat
     1921 gcaatggtag tcctgctctt aaaattgtac ccaatacggg ttggctttgc attaatggaa
     1981 ctgtggcaat gcctaaaggg gtggtcacat cggtgttttc atagatcatg gttttgctta
     2041 tttcgtaagc agcaatttca ccaatacgtt cgagatttct tctaaaacgc atcctatcat
     2101 tctgaatttc aacatcacgc agttctgcta caaaatggtg taataaggag ttgccggtgc
     2161 ttagaatgtt taccataatc atcgtttaaa tagtcttaca ggaaaaggaa gtgtattttt
     2221 tttcaggtgt aaatatacat gttcaatcga accaaaacta ctgtaataac gtgccagtcc
     2281 gaaatcatta gagccttcaa aatcaagaac taaattctta ttggcatttt cctgaattac
     2341 agagtcaatt aataatgaca tggcgcctga ggatttccca gcaacactat ttcctgaaaa
     2401 aagaaaaata attcgataat ctgtctgaat aaacaatcca ccacaaatag tttgatttcc
     2461 atcagaaaca cctcgtaaat aacataatcc tttttgtttc aaagtggagt acaatattct
     2521 tagtaactca taactttcat ctttcattcg ttctattttc atgcctctgt catgtctgaa
     2581 taaacttata acctcggaaa tatcaggttg atttaaactt aattcatttc tttttgcctt
     2641 attaatattt cgcttttgat tatcagagta aaattttctg atttcttcgt aaggtctgtt
     2701 taattccaat tcgaagttta atcttttggc ggcaataaat ttatttccgg ctgccggatt
     2761 aaaagaattt atatttattt caatcagttt aaatttagat ggaatagcat ttaaaaacgc
     2821 atttgaaatt gcaaaatcta taggttgaga agaaaatatt cctaattgtt gtgtaaacag
     2881 aggctggcag agataattga aacatagctt ttttctccat gttaagggaa acacaaagtt
     2941 gtagtcatca cttaccaatg catgccagtt gggactaact gcatcaagat accatgagaa
     3001 agcatacggc agtttattta aagcttgatt gatactttga tcccattttg ctttatcaat
     3061 ctcaatatgt tcaagaaact taacttccat ttcagacagc agctatttta agtaactctt
     3121 cataaatatt tctatagcct ttccagatat gattttcact gagtgattca ttatgccata
     3181 aactgataaa ggtaccattt acannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcta
     3241 ctaatggttt tatcagatca attacctgct caggatttac ttttagataa tatctgaaag
     3301 ttgcatccat aacagcaaac ggatgaatca aaagtggcgt ttgcacttcc tggtccaaat
     3361 cataaaagta aaaggaagag caaatgcctg ccctgaaacc aatatttcct gcatagccca
     3421 tagtgtaatc ctctttaatg taaacactaa taagctgcct gtaagtatca ggtaatctaa
     3481 t
//
LOCUS       DAORQT010000167         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5091_length_3480_cov_11.498686, whole genome shotgun sequence.
ACCESSION   DAORQT010000167 DAORQT010000000
VERSION     DAORQT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_3480_cov_11.498686"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PK134_06280"
     CDS             <1..771
                     /locus_tag="PK134_06280"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRB38491.1"
                     /translation="ILKTARSKAFMTPEGMKKAYDNLCREGIEGIIAIGGDGTLKGAV
                     EFNKVNNIPFIGIPGTIDNDLFGTDYTIGFDTALNTIIDAVDKIKDTAASHDRLFFVE
                     VMGRDSGCLALYSGIASGAEEILMPEQKTDISALVEKLKESVQRRKTSSVVIVAEGDD
                     GGGAFKIAEAVKKSFDYDIKVTVLGHIQRGGSPTAKDRIMATRFGAEAVNALLSGQTN
                     KMTGWNGKEVTLIDLEKVVKQHNPFDKRLVSYNYFLSL"
     gene            794..1642
                     /locus_tag="PK134_06285"
     CDS             794..1642
                     /locus_tag="PK134_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HRB38492.1"
                     /translation="MLLVADSGSTKTNWLLTDRENFTTEIETIGFNPFFHSTDFILNE
                     LKKNQKLVKYADAIKDIKFFGSGCSSDDRKNIVAAAFKIIFPNATLLVDHDMLGAALA
                     ACFDTAGLVAILGTGSNIAFWDGKHLHPTNHGLGYILGDEGSGSYFGRKLMTHFLYGI
                     MPNDLKEKFFERYHLTKESAIKNVYHLAGANVYMASFAKFLSDEASHPYIQNLIYKGL
                     TEFADTSILAYPQHQNYPVHFIGSIAFHFRNILEQIASEKKFIVGKTIIRPVDDLLVY
                     FTQEEK"
     gene            1728..2666
                     /locus_tag="PK134_06290"
     CDS             1728..2666
                     /locus_tag="PK134_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38493.1"
                     /translation="MKKQLFLITSAVVLTTFFYSCKKEDQQALDSKTKQFNDDSNNYK
                     AESDQADNDINNTINEIPAFGRGAGVVSSPLCGVTIDSSQLSQKILFFNFDGITPCFS
                     PSRTRSGQIKVQLTTGTNWHLAGSVLTITYNNFKITRLSDNKSITFNGVKTLTNVNGH
                     NWLTFLAGTSSFKYQSRAFNINVAYDDGSTATWNQANITEWLYVPSGNHGAYLIFTAN
                     GDSTVNNYSNSSMWGTNRYGQAFTTNYTTQIISNTYCGLWRPISGELIHHVNATDFAL
                     TLGVDQSGNASTLDCAYGFKVSWTSNGNSNSVVLSY"
     gene            2743..>3480
                     /locus_tag="PK134_06295"
     CDS             2743..>3480
                     /locus_tag="PK134_06295"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRB38494.1"
                     /translation="MALQEEMEKQGLWLFRYRSTLPLLLLAGGLSYYLWSKMHHIRPF
                     PENSRSEEWFLYVCMAVSFLGLLMRIYIVGHTPANTSGRNTAEGQVADTINQTGMYSM
                     VRHPLYVGNFIMWLGIALFTASIWFVLLFIAVYWLYYERIMYAEEQFMRKKFGNAYTD
                     WAEKTPAFIPEFSNFKKPSLPFSWKKVLKKEKNGFAAVFIVFLLFYAAGQLITNNAIQ
                     NNFLVYGTVFSILFYIVIKILRKQTTLL"
BASE COUNT         1122 a          584 c          663 g         1111 t
ORIGIN      
        1 atccttaaaa cagcacgcag caaggctttc atgactcctg aaggcatgaa aaaggcttat
       61 gacaatcttt gccgtgaagg aatagagggc attatagcta ttggtggtga tggcacattg
      121 aaaggcgcgg tcgagtttaa taaagtcaat aatattccat ttattggtat tccaggaaca
      181 atagataacg atctttttgg aacagattac acaataggtt ttgataccgc attgaatact
      241 ataattgatg ctgtggataa aattaaagac actgcagcat cgcatgacag attgtttttt
      301 gtggaagtta tgggtcgtga ttccggttgc ttagcacttt attcaggaat tgccagtggt
      361 gccgaagaaa tattaatgcc tgaacaaaaa acggacatca gtgctttggt tgaaaaacta
      421 aaagaaagtg tacaaagaag aaaaacttca agtgtggtga ttgttgctga aggtgatgat
      481 ggtggcggtg catttaaaat tgcagaagca gtaaaaaaat cttttgacta cgacattaaa
      541 gttactgtgc ttggtcatat tcaacgtgga ggcagtccaa ctgctaaaga cagaataatg
      601 gctacacggt ttggtgctga agctgtaaat gcacttctca gtggtcaaac caataaaatg
      661 acaggctgga atggtaaaga agttactttg attgaccttg aaaaagtagt taaacaacac
      721 aatcctttcg acaagcgatt ggtgtcatac aattatttct tatctttata aatcatcttc
      781 taatataaac tttatgcttt tagttgcaga cagtggctct acaaaaacaa actggctttt
      841 aacagacaga gaaaatttta ctacagaaat tgaaactatt ggatttaatc cgttttttca
      901 tagtactgat ttcattttaa acgagcttaa aaaaaatcaa aaattagtta agtatgccga
      961 tgcaataaaa gatattaaat tttttggttc gggttgttcc agcgatgaca gaaaaaacat
     1021 tgtagcagca gcatttaaaa ttatttttcc caatgcaaca cttttagttg accacgacat
     1081 gttgggtgca gccttagctg catgctttga cacagcaggg ttagttgcaa ttttaggaac
     1141 aggatcgaac attgcttttt gggatggtaa acaccttcac cctaccaacc atggattggg
     1201 ttatatttta ggtgatgagg gtagcggaag ttattttggc agaaagctca tgacacattt
     1261 tttgtatggt attatgccaa atgatttaaa agagaaattt tttgagcgct atcatctcac
     1321 aaaagaaagt gctattaaaa atgtttacca tctggcggga gcaaatgtat atatggcatc
     1381 atttgcaaaa tttttatctg atgaagcttc gcatccttac atacaaaatc tgatatacaa
     1441 gggtcttact gagtttgccg acaccagtat tctcgcctat cctcaacatc agaattatcc
     1501 ggtacatttt attggcagca tagcatttca ttttcgtaat attttagagc aaattgcatc
     1561 ggaaaagaaa tttattgtag gcaaaaccat tatacgacct gttgatgact tgttggttta
     1621 tttcacacaa gaagaaaaat aaacaatatt gtatataatg tgacgttttt ttaaaaaaca
     1681 aacttctgat gcttaaaatg attactttta ttctaaattc tgtaaaaatg aaaaagcaac
     1741 tctttttaat tacatcagca gtggtactta ccacattctt ttactcctgc aaaaaggaag
     1801 accagcaagc attagattca aagacaaaac agtttaatga tgattctaat aattacaaag
     1861 cagaatctga tcaggctgac aatgacatca acaacaccat taacgaaatc cctgcatttg
     1921 gcagaggtgc tggtgttgta tcaagcccgc tttgtggtgt aacaattgat tcatctcagc
     1981 tttcacagaa aattctgttc tttaattttg atggtatcac accttgtttc agtccgtcac
     2041 gcacacgatc aggtcaaatt aaagttcagt taacaacagg tacaaactgg catctggctg
     2101 ggtctgttct taccattact tacaacaatt ttaaaataac ccgactcagc gataataaat
     2161 ccatcacctt taatggtgtt aaaacactga caaatgttaa cggacacaac tggctaactt
     2221 ttctggccgg tacaagcagt tttaaatacc aaagccgtgc ttttaatatt aatgtggctt
     2281 atgatgatgg ctcaactgcc acttggaatc aggcaaacat tactgaatgg ttgtatgttc
     2341 catcaggaaa tcatggtgct tatcttattt ttactgccaa tggcgattct acagtaaata
     2401 actattcaaa cagcagcatg tggggaacaa acagatatgg tcaggctttt acaaccaatt
     2461 atactacaca aataatttca aacacctatt gtggtttgtg gcggcctatt tcaggtgagt
     2521 tgattcatca tgtaaatgct acagattttg cactcacgtt gggagttgat caaagtggaa
     2581 atgcaagcac actcgactgt gcttatggat ttaaagtatc atggacaagt aatggaaatt
     2641 ctaattcagt agtccttagc tattaacact attttgtttt aaaatgaaaa cagaatctta
     2701 tggtaaggtt ctgtttttat ttttgtgcca tattcaaaca gtatggcctt acaagaagag
     2761 atggaaaaac aagggctatg gttattcagg taccgtagca ccctaccctt actactttta
     2821 gcaggaggct tgtcttatta tttatggagt aagatgcatc acattagacc atttccggaa
     2881 aattcaaggt ctgaagaatg gtttttatac gtttgtatgg ctgtatcgtt tttaggctta
     2941 ttgatgagaa tttatattgt aggacacact ccggcaaaca cttctggtcg caatacagct
     3001 gaaggtcagg ttgctgatac cattaatcaa acaggaatgt attcgatggt tcgtcatcca
     3061 ttgtatgttg gtaattttat catgtggctt ggcatagcat tgtttacagc aagtatttgg
     3121 tttgtcttat tgtttattgc tgtttattgg ctgtactatg agcgtataat gtatgcagaa
     3181 gaacaattta tgcgcaagaa gttcggcaat gcctacaccg attgggcaga gaaaacgcct
     3241 gcctttattc ctgaattttc aaattttaaa aaaccatcac ttccatttag ttggaaaaag
     3301 gttctaaaaa aagaaaagaa tggatttgct gcggttttca ttgtcttttt actcttttat
     3361 gcagctggtc aattaataac caacaatgct attcaaaaca attttttagt ttacggaact
     3421 gttttcagta tcttatttta tatagttatt aaaatcctca gaaagcaaac tacattattg
//
LOCUS       DAORQT010000168         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5141_length_3457_cov_7.875661, whole genome shotgun sequence.
ACCESSION   DAORQT010000168 DAORQT010000000
VERSION     DAORQT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_3457_cov_7.875661"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PK134_06300"
     CDS             complement(<1..197)
                     /locus_tag="PK134_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRB38495.1"
                     /translation="MSELPDNSVHLAITSPPYWQLKDYGTENQIGFHDSYENYINNLN
                     LVWKECYRVLHDGCRLCVNIG"
     gene            complement(441..1745)
                     /gene="sufD"
                     /locus_tag="PK134_06305"
     CDS             complement(441..1745)
                     /gene="sufD"
                     /locus_tag="PK134_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRB38496.1"
                     /translation="MNSSVDTLSVTNETSIAEDNIPVDGLQKLRLAALNKFLEKGFPD
                     SKHEEWKYLNLKPISETKYNHRVVSSPLKASDLEQFYDAGNDTVNLVFINGRYCSEFS
                     GHQSHKHPFSIYSLESREAELYLETLRNSEDEPFHALNTAFHQGGIMIHVPDDCRMPM
                     TIHLLYVNDSQNEAVAAHPKCIVVAGKNSAFQVIATYHSLNNFPSLTNTVTEFLIGEN
                     AVVEFDIKQNENSNANHISRVYARVQRKAVFDIFTVTMGGALVRNNLEIILSEPNACA
                     HLNGLTVADGYQVVDNHTVVDHASPNCESNQLYKNILDDHSQGVFNGKIFVRKDAQKT
                     NAFQSSKNVLLSNTAVMNAKPQLEIFADDVKCSHGATTGQLDEDALFYFRSRGIGERD
                     ARALLNYAFASDVIEKINNDALKSNLLKLLSQKLNTAVDFEE"
     gene            complement(1757..2509)
                     /gene="sufC"
                     /locus_tag="PK134_06310"
     CDS             complement(1757..2509)
                     /gene="sufC"
                     /locus_tag="PK134_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRB38497.1"
                     /translation="MLNIKNLHASVEGREILNGLNLQVNPGEVHAIMGPNGSGKSTLA
                     SVLAGREAYEVNDGEILYHGENLIELSPEERARKGIFLAFQYPIEIPGVSNANFLKTA
                     VNEVRKSKGMDVLDSKDFLKLMKDKMQLVKMDKSMTQRSVNEGFSGGEKKRNEIFQMA
                     MLEPELCVLDETDSGLDIDALRIVSDGINALRDGKRSFIVITHYQRLLDYIVPDFVHV
                     LYKGKIVKSGPKELALELEEKGYDWIKEEVEI"
     gene            complement(2555..>3457)
                     /gene="sufB"
                     /locus_tag="PK134_06315"
     CDS             complement(2555..>3457)
                     /gene="sufB"
                     /locus_tag="PK134_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRB38498.1"
                     /translation="ALNSAVFSDGSFCYIPKGVRCPMELSTYFRINSAGTGQFERTLI
                     IADEGAYVSYLEGCTAPKRDENQLHAAVVEIYAHTDAQVKYSTVQNWYPGDKDGRGGI
                     FNFVTKRGLCAGERSKISWTQVETGSAVTWKYPSVILKGDYSTGEFYSVALTNNYQQA
                     DTGTKMIHIGKHTRSTIISKGISGGRSNNSYRGLVRIGKQAEKARNFSQCDSLLLGDK
                     CGAHTFPYLECANPTAKVEHEATTSKVGEDQIFYCNQRGISTEDAIGLIVNGYCKEVF
                     NQLPMEFAVEAQKLLAVSLEGSVG"
BASE COUNT         1017 a          745 c          552 g         1143 t
ORIGIN      
        1 tcaccaatgt tcacacataa cctacaaccg tcatgcaaaa cacgataaca ttctttccat
       61 actaaattca ggttattgat ataattttca taactatcat gaaagcctat ctgattttct
      121 gtaccatagt cttttaactg ccaataaggt ggtgatgtta ttgccaaatg aacagaatta
      181 tcaggcaact cagacatgtg tctgctatct ccgctaatta ttttatgatt cgtcttcaag
      241 tttctaactt acaagtttat cttggtcaaa tttaaacaaa atgttaagct aaacttataa
      301 gttttcttaa attttctgcc tgaatcgtaa tagattcact tcataacaat caaatggaaa
      361 ttatacaaac atttctacac taaatgtaag ttcttatttg atgccaatag tcccgtttaa
      421 attacactaa taaacttaaa ctactcctca aaatcaaccg ctgtatttag tttttgcgat
      481 aacagtttta acaagtttga tttaagtgca tcgttattga ttttctcaat aacatcactg
      541 gcaaatgcat aattcagcaa agcacgagca tcacgttcgc caataccacg cgaacggaaa
      601 taaaacaatg catcttcgtc caactgacct gtggttgctc catgtgaaca ttttacatca
      661 tcagcaaaaa tttcaagttg tggctttgca ttcatcacag cagtgttact caacaacaca
      721 ttcttactgc tctgaaaagc attggtcttc tgtgcatctt tccgaacaaa tatctttccg
      781 ttaaaaactc cctgcgaatg atcgtctaag atatttttgt acaattgatt gctctcgcag
      841 tttggacttg catgatctac cactgtgtga ttatctacca cctgatagcc atcagcaaca
      901 gtgagtccgt taagatgagc acaggcattt ggttcactta aaataatttc gaggttgtta
      961 cgaactagtg caccacccat cgtaacagta aaaatatcga acacagcttt acgttgtaca
     1021 cgggcataaa ctctgctaat atggttggca ttactgtttt cattctgctt aatatcgaat
     1081 tcaacaactg cattttcacc aataaggaac tctgttactg tattggtgag tgatggaaaa
     1141 ttattgagtg aatgataagt tgcaattacc tgaaatgctg aattttttcc tgcaacaaca
     1201 atacattttg gatgtgctgc aacggcttca ttctgcgagt cgttaacata taggagatgt
     1261 attgtcattg gcatacggca atcatcagga acatgaatca taataccacc ttgatgaaag
     1321 gctgtattta atgcatgaaa aggttcgtct tctgaattac gcaaagtctc taaatacagt
     1381 tctgcttctc tgctttcgag cgaataaata ctgaaaggat gtttgtgact ttgatgtcct
     1441 gaaaactcag aacaataacg tccgtttata aaaaccaaat tcacagtatc gttgcctgca
     1501 tcgtaaaatt gctccaagtc tgaagctttc agtggcgatg acactacacg gtgattatat
     1561 ttagtttcac ttataggttt caaattaaga tatttccact cttcatgctt tgaatcagga
     1621 aatcctttct ctaaaaattt atttaatgct gcgagtcgca atttttgcaa tccgtcaaca
     1681 gggatattat cttcggcaat gcttgtttcg ttagtcacag aaagtgtatc aacagatgag
     1741 ttcacaataa tcaggtttaa atttcaactt cttcttttat ccaatcatat cctttttctt
     1801 ccagctcaag agcaagttct tttggtccgc tcttaacaat ttttccttta tacaaaacgt
     1861 gtacaaaatc gggaactatg taatcgagca atcgctgata gtgtgtaata acaatgaaag
     1921 aacgtttgcc gtcacgcaat gcattgatac catcggatac aatcctaagt gcatctatat
     1981 ctagacctga atcggtttca tcgagtacac ataactcagg ctcaagcatg gccatctgaa
     2041 aaatttcatt gcgtttcttc tctcctccgc tgaatccttc attaacactg cgttgtgtca
     2101 tgcttttatc catcttcacg agctgcattt tatctttcat caactttaaa aagtcttttg
     2161 aatcgagtac atccatacct ttcgacttac gcacttcgtt aactgctgtt ttcagaaagt
     2221 tggcattact cacaccggga atttcaatcg gatattgaaa tgccagaaat attcctttac
     2281 gggctctttc ttcaggagaa agttctatta ggttctcacc atgatacaat atttcaccat
     2341 cattaacttc atacgcttcg cgtcctgcaa gtaccgatgc aagcgtactt tttcctgaac
     2401 cattaggtcc cataatggca tgcacttctc ccggatttac ctgaagattt aatccgttta
     2461 atatttctct accttcgact gaggcatgta gatttttaat gtttaacata ctttctaaaa
     2521 aactttattc ttcttatcta actatattta atttttatcc tactgatcct tctaatgaaa
     2581 ccgctaatag tttttgtgct tctacagcaa actccatggg caactgatta aacacttctt
     2641 tgcaataacc atttactata agtccaattg catcttctgt agagatacct cgttgattgc
     2701 aataaaatat ctgatcttct ccaacctttg atgttgtagc ctcatgctca actttagctg
     2761 tcgggtttgc acactccaaa tatggaaatg tatgtgcacc acatttatca cccaacaaca
     2821 aagaatcgca ttgtgaaaaa tttcttgctt tctcagcctg tttaccaata cgtaccaatc
     2881 cacgataact attgttactg cggcctccgc ttattccttt actgatgatg gtgctgcgtg
     2941 tatgtttacc gatatgaatc atctttgtac cggtgtcggc ctgctggtag ttgttggtta
     3001 atgcaacaga atagaattca ccagttgaat agtcaccttt taatatcaca cttggatatt
     3061 tccatgtaac agcactaccc gtttcaacct gtgtccaact gattttagaa cgttcgcctg
     3121 cacaaagtcc acgcttggta acaaaattaa aaataccccc acgaccatct ttgtcaccgg
     3181 gataccagtt ttgtacagtg ctatacttta cctgtgcatc ggtatgtgca taaatttcta
     3241 ctactgcagc atgcaattga ttctcgtcac gctttggtgc tgtgcaacct tcaagatagc
     3301 taacataagc accttcatcg gcaatgatta gtgttctttc aaactgacct gtgcctgcag
     3361 agtttatacg gaaataggta ctcaactcca ttgggcaacg tacacctttg ggtatataac
     3421 aaaaagagcc gtcactgaaa acggcactgt ttaatgc
//
LOCUS       DAORQT010000169         3394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5291_length_3394_cov_4.599581, whole genome shotgun sequence.
ACCESSION   DAORQT010000169 DAORQT010000000
VERSION     DAORQT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_3394_cov_4.599581"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK134_06320"
     CDS             complement(<1..364)
                     /locus_tag="PK134_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38499.1"
                     /translation="MYENLSNHAIITLVGGARPMDIVLYGTLVNTDRDLVIQTRSGYM
                     GGAFNLGINQTKVIINDVPSMLFLNRNNVDPSGVPDAVWSNILKTGQLPDGHYNFCVE
                     AFEITSAAGLISAGSSCYE"
     assembly_gap    377..419
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(546..2270)
                     /locus_tag="PK134_06325"
     CDS             complement(546..2270)
                     /locus_tag="PK134_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38500.1"
                     /translation="MQKKSQNEKKSYEISLSLFLLFLFFTIISKAQDVEHINLKKPVT
                     FHGNLNFQLDYYSANGIPARQKEFSWIINGNPVLNVLGIDLPFTFLFSNFGNKYSQPF
                     NQFGLSPRYKWITMHLGYRNLTYSNYTLAGHKMLGVGFDLNPKKFRIGFMYGQLRRSA
                     SIDSTMNTNPMYVRSTPTFKRVGIAGKIGYGSQKNYVDLVYFKGWDKPSSLNGKLKDS
                     IQPAENTSFGLVSTLSLSKKITWTSDIGFSAYTLNRMDRKDSSDIDKAWPKDIMGPLV
                     SDKLSTQYYFAGETKIGYQEKKWGAQLKLKRIDPGYQSMGAYFFQNDIKEYSLANNFK
                     LDSNRLNINTNIGIQTDNLKKQKSSTSKRFIGSVNVNYVPSQKFGISFNYSNFGITNS
                     PLQTSPGNEQYKQVNNSVMLMPYLLWTNEKTFKNLNCIATFQSLSTPQSYGNNTPDMN
                     TFSFSTNYSQTWIKPGINANVAMNFINSKTTAGDVGSIGGSVGGSAPLMKKRLTLNTS
                     FSYLQNKFKGNDNGNTIRFTLGFMVPVGTHHNFQLMCNYLDNTSKDVSLIQNFKETNI
                     QFIYGLTF"
     gene            complement(2540..2941)
                     /locus_tag="PK134_06330"
     CDS             complement(2540..2941)
                     /locus_tag="PK134_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38501.1"
                     /translation="MLKDIPQLKVENVAVAIVKEQSNGADVWNAYIINLKNEMLEGVL
                     ITSKGYGMMEGEERKTSTLRHFIENIPPQSCVKIEPITDNVLNLNNEYWVSFYLNNTM
                     YDRKYVFLPGVINEEFFTQIPLLEKRGVMIK"
     gene            complement(2988..>3394)
                     /locus_tag="PK134_06335"
     CDS             complement(2988..>3394)
                     /locus_tag="PK134_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRB38502.1"
                     /translation="QWSLDHKDGIEKAYCCFYSNARDFARFGKLVLQHGRWNNEQIID
                     SAFITLCTTPNNLPDKEDNKPVQHYGYQWWLSNISNHPVYYMRGILGQYVIVIPDMQM
                     IIVRLGHKRSNNPDGKPNDYKFYAEQVIKMYE"
BASE COUNT         1088 a          590 c          525 g         1148 t
ORIGIN      
        1 attcatagca agaactacct gctgaaatta agcctgcagc cgaagttatt tcaaatgcct
       61 caacacaaaa attataatgt ccatcgggca gctgtcctgt tttcaaaata tttgaccaca
      121 cagcatcagg aactcccgaa ggatccacat tattcctgtt taaaaaaagc atagaaggaa
      181 catcattgat tataaccttg gtctgattaa ttcctagatt aaatgcacca cccatgtatc
      241 ctgatcttgt ttgaataacc aagtctctgt ctgtattaac caatgtgccg tacaatacaa
      301 tatccattgg tcttgcccca cccactaaag ttattattgc atggttcgat aaattttcat
      361 acaaactgta attagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
      421 attgaagtca gaaatgaatt ctgactcatt acccacatag gaaacattag taaataaaat
      481 ataaaataac ttttcctgaa tatgtttttt gtgcctaaac tacaagtgtt cattgttact
      541 actttttaga aagttaatcc ataaataaac tgtatgttgg tttctttaaa attctgtatt
      601 aaggaaacat cttttgaagt gttgtctaaa taattacaca tcaattgaaa gttatgatgt
      661 gttcctactg gaaccataaa acctaatgta aatcttatgg tattaccatt atcatttccc
      721 ttaaatttgt tttgcagata agagaaagaa gtatttagag tgagtctttt tttcataagt
      781 ggtgctgatc caccaactga tccaccgata gaacctacat caccagcggt agtttttgaa
      841 ttaataaaat tcatagcaac atttgcatta ataccaggct ttatccaggt ttgagaatag
      901 ttagtagaaa aagaaaaagt attcatatca ggtgtgttat tgccataact ctgtggagtg
      961 cttaaagatt gaaaagttgc aatacaattc agatttttaa aagttttctc atttgtccag
     1021 agtaaataag gcattagcat tacactgttg ttaacctgtt tatactgttc attgccaggt
     1081 gaagtctgca atgggctgtt ggtaatacca aagttgctgt aattgaaact tattccaaat
     1141 ttctgcgatg gaacataatt aacattaaca cttccaataa atctttttga agtagaagac
     1201 ttctgttttt tcaggttgtc ggtttgaata ccaatgttgg tgtttatgtt caacctatta
     1261 ctatccaact taaaattatt tgctaaactg tactccttta tatcattctg aaaaaaatag
     1321 gctcccatag attgataacc cgggtcaatt cttttaagct tcaattgggc accccatttt
     1381 ttttcctgat agccaatctt ggtttctcct gcaaaataat attgtgtgct gagtttatca
     1441 ctcactaaag gtcccataat atcttttggc caagccttat caatatcact tgaatccttt
     1501 ctgtccattc ggttaagtgt atatgcacta aaaccaatat ctgatgtcca cgtaattttt
     1561 ttcgataatg acaaagtgga aaccagtcca aatgaagtgt tttctgcagg ttgaatagaa
     1621 tcttttaatt ttccattaag tgaagaaggc ttatcccatc ctttaaaata aaccagatca
     1681 acataattct tttgtgagcc ataaccgatt ttacccgcta tgccaacacg tttaaaagta
     1741 ggtgttgaac gcacgtacat tgggtttgtg ttcatagtgg aatcaataga tgctgatcgc
     1801 cttagttgtc catacataaa tcctattcgg aacttttttg gattcaaatc aaatcctaca
     1861 cctaacattt tgtggccggc caacgtatag tttgaatatg tgaggttacg atagcctaaa
     1921 tgcatagtaa tccatttgta tcgtggactc aatccaaatt gattaaatgg ctgcgaatat
     1981 ttattgccaa aatttgaaaa aagaaaagta aatggcaggt caattcccaa tacatttaaa
     2041 acaggattgc cattgataat ccaagaaaat tctttttgcc ttgcaggaat accattggca
     2101 ctgtaataat ccaattgaaa attaagattt ccatgaaatg ttacaggctt tttcagatta
     2161 atgtgttcaa catcctgcgc cttactaatt atggtaaaga ataaaaaaag taaaaataga
     2221 gataatgaaa tctcataact ttttttttca ttttgtgatt ttttctgcat gcggatatat
     2281 tgaaaaaaaa tcaatcgtaa aaatcactca attaagttgt ttaattctga tataccatta
     2341 atcacatata aacaactcaa tcaatcacaa attactttcg tgttagggta tttagaaagt
     2401 aataagcaaa tgtaattgtt ttaagaaatt aaaaaggaaa ttttctaaat atttttaact
     2461 gagttataat tacatcctgt taaaattcct atattgattc gaagacttat aaaggggtct
     2521 ataataaatt tcttctaaat cacttaatca tcaccccgcg tttttcgagt aagggtatct
     2581 gtgtaaaaaa ttcttcattt ataactcccg gaagaaaaac atactttcga tcgtacatgg
     2641 tgttgttgag gtaaaagctt acccagtatt cattattcag gttaagaaca ttatctgtaa
     2701 taggttcaat ctttacgcac gactgcggag gaatattttc tataaaatgt ctaagcgttg
     2761 atgtttttct ttcttcacct tccatcatgc catagccttt gcttgtaatc aacacacctt
     2821 ccagcatttc atttttcagg ttaatgatat aggcattcca aacatcggca ccgtttgatt
     2881 gctcttttac aattgcaacg gctacgtttt caacttttaa ttgaggaata tcttttagca
     2941 tattatatta aacgagtaat gataatagaa gttaccttga tagcttgtta ttcatacatt
     3001 tttatgactt gttcggcata aaatttatag tcattaggct ttccgtccgg attgttactg
     3061 cgtttatggc ctaaacgcac aataatcatc tgcatatcag gaataacaat aacatactgc
     3121 cctaagatgc cacgcatgta atacacagga tgattgctga tgtttgacaa ccaccactga
     3181 tagccgtagt gctgaacagg tttattatct tctttatccg gtaaattatt tggtgtggta
     3241 cagagagtaa taaaagctga atcaataatt tgttcattat tccatctgcc atgttgcaaa
     3301 acaagtttac cgaaacgtgc aaagtcgcga gcattgctgt aaaaacaaca ataagctttt
     3361 tctattccgt ctttatgatc gaggctccat tgtg
//
LOCUS       DAORQT010000170         3392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5294_length_3392_cov_4.566377, whole genome shotgun sequence.
ACCESSION   DAORQT010000170 DAORQT010000000
VERSION     DAORQT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_3392_cov_4.566377"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>373)
                     /locus_tag="PK134_06340"
     CDS             complement(<1..>373)
                     /locus_tag="PK134_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRB38503.1"
                     /translation="MLDGKFDEAMSFFSFNPSQKTLLIVGGSQGARSINKSILAGLEK
                     LKAAGVQLIWQTGKLFYEEAQRAVAEVGYGQARVFDFIARMDFAYAAANLVVSRAGAS
                     TVSELTLTGKPYTLANQERVAI"
     assembly_gap    374..383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<384..>708)
                     /locus_tag="PK134_06345"
     CDS             complement(<384..>708)
                     /locus_tag="PK134_06345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38504.1"
                     /translation="GIKRELTIDNLSFPFKVLSSISKAMHILKEFKPNAAVGVGGYAS
                     GPLLYAASLKKIPCLIQEQNSYPGITNKILAKRVQKICVAYEGMDKYFPASKIILTGN
                     PVRENV"
     assembly_gap    709..723
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<724..912)
                     /locus_tag="PK134_06350"
     CDS             complement(<724..912)
                     /locus_tag="PK134_06350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38505.1"
                     /translation="MQQPDIKVIISGGGTGGHIFPALAIANAIKSARPQAEILFVGAK
                     GRMEMEKVPAAGYKIEGLW"
     gene            complement(899..2056)
                     /locus_tag="PK134_06355"
     CDS             complement(899..2056)
                     /locus_tag="PK134_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRB38506.1"
                     /translation="MNWIETYFKGDRIIWIVVILLSLMSLMAVYSSTGTLAYKYQEGN
                     TEYYLFKHGIIVFLGFGIMYFTHLVKYTFYAKVSRIAIFLAIPLLFLTLLTGTNLNEA
                     SRWLTLPVINLSFQTSDLAKIALLLYVARSLSKKQDNIKDFKSAFLPVILPVCIICGL
                     ILPANFSTAAVLFTTCLVLMFFGRINFKYILSLVGIGIAGLGLFLLVASFSGYEGRIG
                     TWKKRIENFSNGNDQGNYQTQQAKIAIASGGVFGKGPGQSTQRNFLPHPYSDFIFAII
                     IEEYGFVGGLVIVVLYLLLFYRAVVIVRKSPFAFATLLAIGCAFSLVFQAMINMAVAV
                     NLFPVTGQPLPMLSMGGTSIWFTSIAVGIILSVSRNIEQEELKTGVVHAAA"
     assembly_gap    2150..2159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2160..3384)
                     /gene="murD"
                     /locus_tag="PK134_06360"
                     /pseudo
     CDS             complement(2160..3384)
                     /gene="murD"
                     /locus_tag="PK134_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275190.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
BASE COUNT         1038 a          726 c          589 g         1004 t
ORIGIN      
        1 gaatagccac acgttcttgg tttgcaagag tataaggttt acctgtcaaa gtaagttcag
       61 aaactgtgct tgcccctgca cgcgacacca ctaagtttgc agcagcatag gcaaaatcca
      121 ttcttgcaat aaaatcaaaa acacgtgctt gcccatagcc tacttccgca acagcacgct
      181 gtgcctcttc ataaaacagt ttgcccgttt gccatatcag ttgcactcca gcagctttca
      241 gcttttctaa tccggcaaga atacttttat tgatagagcg tgcgccctga ctgccaccta
      301 caatgagcaa agttttttgt gaaggattaa aactaaaaaa agacattgcc tcatcaaact
      361 ttccatcaag catnnnnnnn nnncacattt tctctaaccg gatttccggt gagaattatt
      421 tttgatgcag ggaaatattt atccatgcct tcataggcaa cacatatttt ctgaacacgt
      481 ttggcaagaa ttttattggt gatacctgga taggaattct gctcctgaat gagacaagga
      541 atttttttta atgatgcagc gtaaagtaaa ggtccgctgg cataaccacc tacacctaca
      601 gctgcatttg gtttaaactc ctttaaaata tgcatagcct tactgatgct tgacaacacc
      661 ttgaacggaa atgacaggtt gtctattgtc agctctcgtt ttattccgnn nnnnnnnnnn
      721 nnnccaaagt ccttcaattt tataaccggc agcaggaact ttttccattt ccattcggcc
      781 tttagcacca acaaaaagta tttcagcctg aggtcttgca cttttaattg cattggcaat
      841 ggcaagtgca ggaaaaatat gtcctcctgt acctcctccg ctgatgataa cttttatgtc
      901 aggctgctgc atgtactact cctgttttta actcttcctg ctcaatattc cggctgacac
      961 taagaataat accaacggca atgctggtaa accaaataga tgttccgccc atactcaaca
     1021 tcggcaatgg ctgccccgtt accggaaaaa gatttactgc aactgccata tttatcatag
     1081 cctggaatac taggctgaat gcacagccta ttgcaagcaa tgttgcaaaa gcaaatggac
     1141 tctttcgaac aatgacgaca gcccgataaa acagcaacag ataaagaaca actattacca
     1201 aaccaccaac aaaaccatac tcctctatta ttattgcaaa aataaaatcg gaataaggat
     1261 gtggaagaaa atttctttgc gtactctgtc cgggtccttt accaaaaaca ccacctgagg
     1321 caattgcaat ttttgcctgt tgtgtctgat agtttccctg gtcatttcca ttgctgaaat
     1381 tttcaatccg ttttttccat gtaccaattc tcccttcgta tcctgaaaat gaggcaacca
     1441 ataaaaacaa acccagccct gcaataccga tacctacaag agaaagtata tacttgaaat
     1501 ttatacgacc gaaaaacatc agcacaagac aggtggtaaa caatactgct gcggtagaaa
     1561 agttagccgg aagaattaat ccacagatga tacaaacagg aagaattaca ggaagaaaag
     1621 cagacttaaa gtctttgatg ttatcctgtt ttttagaaag cgatcgtgct acatacaaca
     1681 acaatgcaat cttagctaag tcagatgtct gaaacgaaag attgattacg ggcagtgtca
     1741 gccaacgtga tgcttcgtta aggttagtac ctgtaagaag tgtaagaaac aaaagtggaa
     1801 tagcaagaaa aattgctatg cgtgaaactt ttgcatagaa agtatatttt acaagatgtg
     1861 taaaatacat tataccaaaa ccaagaaaaa caataatacc atgcttaaat aaataatact
     1921 ctgtattacc ttcctgatac ttatatgcca aagtgcctgt ggaactataa acggccataa
     1981 gtgacattaa cgaaagcagt attaccacaa tccagatgat gcggtcgcct ttaaaatatg
     2041 tttctatcca attcattttt tttgaattaa atcttaaagt gcttttacag cagctttaaa
     2101 ctgacgacca cgatcctcgt aatttttaaa caaatcgaaa cttgcgcagn nnnnnnnnnc
     2161 aacaatacta catcaccttt tttgcctttc tgataggcaa cgcttactgc ttcctgcgca
     2221 gacaaagtat cagtaatatc gcttacaata cttccgaatg cttcatgtat ttttttattg
     2281 tctttaccaa gacaaacaat agctttcact ttctctttta caagatcgtt cagcatttcg
     2341 tactcattac ctttatcaac accgcccaga atccaaacaa cagttaacgt ttgttgcctt
     2401 tgaatcgttg ataaagtcaa taccgtgtac ggtatttacg aactccatcc gatgttctgc
     2461 attgcgaaaa tctgcaaagc tttcgcggat agactccttg cgaatgtcga gtatgcgtgc
     2521 tgcaactccg gcagccatac tgttgtagag gttgtgtttg ccctgtaagg ctagttcgaa
     2581 aatagacata gtaaataagt tgttgtgtat gttcagatat aagttattgt catttacaaa
     2641 agcactcatg ccttcatcct gcctgattga aaaaggatag cacgttgcca aaggatttct
     2701 tttcagaact tctgttttta caactgcatc atcgaagcaa tagataaatg catcttcggc
     2761 agtttgattc tgtatgattc tgaatttagc gtcaatataa ttttgtattg aatactcgta
     2821 acggtcaaga tgatcgggcg tgatgttgag taacacagca atatcagctt tgaaggtgta
     2881 catatcatct aactgaaagc tgcttaactc aagcacataa tggtcaaaat tttcttctgc
     2941 aacctgccag gcaaaactct tacctatatt tccggcaaga cctacattaa cacctgcctt
     3001 tttaagaata tgataggtca gtaaagttgt tgttgacttg ccattggtgc ctgtgatggc
     3061 aatcatttta gcattggtaa accttgaagc aaattcaatc tctgaaacta tattgatgtt
     3121 tgcactctta atttttttaa tcactgctgc cttatcagga ataccggggc ttttaactac
     3181 ctcatctgca ttaagaatca gactgtcggt atggcaacct tcttcaaact gaatattccg
     3241 ctctgtcaga tgtgttttgt aattgtcggc aatacgcgat aggtctgata caaaaacatc
     3301 atagccttgc ttctgcgcaa gtatggctgc gcctactccg ctttcggcat cacccaaaat
     3361 aactatgcga ttgttttttg acatttaact at
//
LOCUS       DAORQT010000171         3361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5378_length_3361_cov_7.365699, whole genome shotgun sequence.
ACCESSION   DAORQT010000171 DAORQT010000000
VERSION     DAORQT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5378_length_3361_cov_7.365699"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK134_06365"
     CDS             <1..470
                     /locus_tag="PK134_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB38507.1"
                     /translation="ALMSKVMTNGNGYIKFPINEPAIGKKKSQVEEYLDFYEGAGCQH
                     IAVATDDIVHTITEMRKRGVEFLYVPGSYYDTVKERVGIIEEDLEELKKWGIMVDRDE
                     EGYLLQIFTKPVEDRPTLFYEIIQRKGAKSFGKGNFKALFESIEAEQARRGTL"
     gene            482..1429
                     /gene="paaA"
                     /locus_tag="PK134_06370"
     CDS             482..1429
                     /gene="paaA"
                     /locus_tag="PK134_06370"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRB38508.1"
                     /translation="MTEEEKEKQFQQRIDAGEKIEPRDWMPEAYKQTLFRQISQHAHS
                     EIIGMQPEGNWLLRAPSLRAKKILLAKIQDEAGHGLYLYSAAETFGIDRTEMIAMLHS
                     GKAKYSSIFNYPTLTWADMGAIGWLVDGAAIVNQTMLAKCSYGPYSRAMIRICKEEGF
                     HQRQGYEIIAKMMNGTTAQRNMAQDSLNRWWEPSLMMFGPHDSESTHSKDSMKWKIKV
                     ESNDTLRQRFIDRTVEQAHHIGLTIPDKNLKYNKTTMHWETSEINWDEFWSVVKGNGP
                     CNYQRISHHIHYHNEGKWVRDCATAFEKSREKENSSISI"
     gene            1441..1731
                     /gene="paaB"
                     /locus_tag="PK134_06375"
     CDS             1441..1731
                     /gene="paaB"
                     /locus_tag="PK134_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRB38509.1"
                     /translation="MKNDYQFPQWEVFIQTKNGGPFEHQGSLHAADKKMALQNARDLY
                     TRRNEGRSIWVVPTECIAATTADDAADFFDPANDKIYRTPNFYKMPQGTKNL"
     gene            1734..2492
                     /gene="paaC"
                     /locus_tag="PK134_06380"
     CDS             1734..2492
                     /gene="paaC"
                     /locus_tag="PK134_06380"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRB38510.1"
                     /translation="MKTKDALYNYCLRLADSNMIHGQRLAEWCSRGPFLEEDIALSNM
                     SLDMFGQAEMLYEYASSLSEDELSADDIAFRRSEREYFNCLLVEQENGDFAQTMMKVF
                     LYSAFAKQLYESLLNSNDETLSGFAAKAIKEVKYHLRHSSEWLIRFGNGTDESKMRVQ
                     QAVTNLWRFTQDMFEMNDADTALIDNGISFTTDTLFAKWQLNINEIFAAANINTPEVN
                     NTITGGHNGIHTERLGVMLCEMQYLQRAYPDAKW"
     gene            2504..3013
                     /gene="paaJ"
                     /locus_tag="PK134_06385"
     CDS             2504..3013
                     /gene="paaJ"
                     /locus_tag="PK134_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRB38511.1"
                     /translation="MSSVKNTESSKSTESLIWALLHTVPDPDIPVISIVDLGIVRNVE
                     WKNEKAIITITPSYSGCPAMSVFVDDIKQVLNGAGFQSEIKTILSPAWTTEWISQSGL
                     DKMKAHGIAPPEDETGMDSIFAMQKPVTCPRCNSKQTKMLNRFGSTPCKALWYCNECS
                     QPFEYFKCH"
     gene            3013..>3361
                     /locus_tag="PK134_06390"
     CDS             3013..>3361
                     /locus_tag="PK134_06390"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRB38512.1"
                     /translation="MMNETVRVTKIFVFDMAHALFGYDGPCKNIHGHTYKLSVTLKGK
                     PFNDNSNPKAGMVIDFSILKQIVQEKIIKQFDHALVLNNDSPHKLLDKVLTQQFEKII
                     YLDVQPTCENLLLH"
BASE COUNT         1174 a          589 c          701 g          897 t
ORIGIN      
        1 ctgctctgat gagtaaggta atgaccaatg gcaatggtta tattaaattt ccgattaacg
       61 aacctgccat cggcaaaaag aaatcgcagg tagaagagta tcttgatttt tatgaaggtg
      121 caggctgtca gcatatagca gtagcaacag atgatattgt tcacactatt acagaaatgc
      181 gcaaacgtgg tgtagaattt ttatatgtgc cgggaagcta ttacgacacc gtcaaagaac
      241 gtgtaggaat tattgaagaa gatttggaag aactgaaaaa atggggaatt atggttgacc
      301 gtgatgagga aggttattta ttacaaatat ttaccaaacc tgttgaagac cgaccaacat
      361 tattctatga aattatacag cgaaaaggtg ccaaatcttt tggcaaagga aattttaaag
      421 ccttgtttga aagtatagaa gcagaacagg caagacgagg aacattataa tttttaaagt
      481 tatgacagaa gaagaaaaag aaaaacagtt tcagcaaaga attgatgcag gtgaaaaaat
      541 agaaccacgt gattggatgc ctgaagcata taaacaaact ttattcagac agatatcgca
      601 acatgcacac tcggaaatta taggtatgca acctgaggga aactggctat tgcgtgcgcc
      661 ctcattacgt gcaaaaaaaa ttctgcttgc caaaatacag gacgaagccg gacatgggct
      721 ttatctctat tcagcagccg aaacatttgg tattgacaga acagagatga ttgctatgct
      781 acactcagga aaggcaaagt attcaagtat ttttaattat ccaacattaa cctgggccga
      841 catgggtgca attggttggt tggttgatgg agcagctatt gtaaatcaaa ccatgctggc
      901 aaagtgcagc tacgggcctt attcgcgtgc aatgattcgc atctgtaaag aagaaggatt
      961 tcatcagcgc caaggctatg agattattgc aaagatgatg aatggaacaa cagcacaacg
     1021 caacatggca caagattcat tgaaccgttg gtgggaacca tcactgatga tgtttgggcc
     1081 acatgattct gaatccacac attcaaaaga ttcaatgaag tggaaaataa aagttgaaag
     1141 taatgacaca cttcgtcagc gttttattga cagaacagtt gagcaggcac accacatcgg
     1201 acttacaata cctgataaaa acttaaagta caacaaaaca accatgcact gggaaacaag
     1261 tgaaattaac tgggatgaat tctggagtgt ggtaaaagga aatggcccat gtaattatca
     1321 gcgtataagt catcatatcc attatcataa tgaaggaaaa tgggttcgcg attgtgcaac
     1381 tgcatttgaa aaaagcagag agaaagaaaa ttcttccata tcaatttaaa aaattataga
     1441 atgaaaaacg actatcagtt tcctcaatgg gaagtattta tacaaacaaa aaacggtgga
     1501 ccttttgaac atcagggaag cctgcatgca gctgacaaaa aaatggctct gcagaatgca
     1561 agagatcttt acacacgcag aaatgaagga cgtagcattt gggtagtgcc tactgaatgt
     1621 atagctgcaa ctactgctga tgatgctgct gacttttttg atccggcaaa cgataaaatc
     1681 tatcgaacac caaactttta taaaatgccg caaggaacaa aaaatctgta atcatgaaaa
     1741 caaaagacgc attatataac tattgcctgc gattagccga cagcaatatg attcatggcc
     1801 aacgtttggc tgaatggtgc agcagaggac cttttttaga agaagatatt gctttatcaa
     1861 atatgtcatt agacatgttt ggtcaggctg aaatgcttta tgaatatgca tcatcattat
     1921 cggaagatga acttagtgct gacgatattg ctttcagacg cagtgaaaga gaatatttca
     1981 actgcctttt ggtagaacag gaaaatggcg actttgcaca aacgatgatg aaagtgtttt
     2041 tgtattctgc atttgcaaaa caactttatg aatcgttgtt gaacagcaat gatgaaacat
     2101 tatctggttt tgctgctaaa gctattaagg aagtaaaata tcatttgcga cacagcagcg
     2161 aatggctcat cagattcgga aacggaactg atgaaagtaa aatgcgtgtg cagcaagctg
     2221 tcacaaattt gtggcggttt acacaagata tgtttgaaat gaatgatgca gacactgcat
     2281 taattgataa cggtatctct tttacaacgg atacgctatt tgcgaagtgg caactaaaca
     2341 taaatgaaat attcgctgcg gcaaatatta acactccgga agtaaacaac accataacag
     2401 gtggccacaa tggtattcat acagaacgct taggtgtcat gctttgcgaa atgcaatatt
     2461 tgcagagagc ctatcctgat gctaagtggt agaataaaaa gtcatgtcat cagtaaaaaa
     2521 tactgaaagc agcaagtcaa cagaatcatt aatctgggca ttattgcata ctgttcctga
     2581 tcctgatatt cctgttatca gcattgttga tttaggcatt gtccgcaatg ttgaatggaa
     2641 aaacgaaaaa gcaattatca caattacccc aagttattcg ggctgtcctg ccatgtctgt
     2701 ttttgtggac gacatcaagc aggtattaaa tggtgcaggg tttcagtcag aaataaaaac
     2761 tatactatca cccgcctgga ctacagaatg gataagtcag tcaggacttg acaagatgaa
     2821 agcccatgga attgcacccc ctgaagatga aaccggaatg gatagtatat ttgcaatgca
     2881 aaaaccggtt acctgccccc gttgcaacag caaacaaact aaaatgctga atcgctttgg
     2941 ctcaacacca tgcaaagcac tttggtattg caacgaatgt agtcagccgt ttgagtattt
     3001 taaatgtcat taatgatgaa tgaaactgtt cgggtaacta aaatttttgt gtttgacatg
     3061 gctcatgcct tatttggcta cgatggtcca tgtaaaaata ttcatggtca cacctataaa
     3121 ctttctgtca ccttaaaggg aaaacctttt aatgacaata gcaatccgaa ggctggtatg
     3181 gtcattgatt tttcaatatt gaaacaaata gtacaggaaa aaattatcaa acaatttgat
     3241 catgccttag tactcaacaa cgattcgcca cataagctgc ttgacaaagt acttacacaa
     3301 cagtttgaaa aaattattta tttagatgtg cagccaacct gcgaaaatct gttgttgcat
     3361 t
//
LOCUS       DAORQT010000172         3335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5441_length_3335_cov_4.094817, whole genome shotgun sequence.
ACCESSION   DAORQT010000172 DAORQT010000000
VERSION     DAORQT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5441_length_3335_cov_4.094817"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>354
                     /locus_tag="PK134_06395"
     CDS             <1..>354
                     /locus_tag="PK134_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRB38513.1"
                     /translation="IVVDVAQAVVHEPLNVQKLDCDFLCFSSHKMLGPTGTGVLYGKA
                     KWLNDMPPYRGGGDMIKSVSFEKTIYNDIPFRFEAGTPNIADVIAFGKAIDYLNSIDR
                     IVALNYEMGLAKKAS"
     assembly_gap    355..364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <365..647
                     /locus_tag="PK134_06400"
     CDS             <365..647
                     /locus_tag="PK134_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRB38514.1"
                     /translation="LLSAIEGVRLIGTAENKTSVVSFIIEGVNAMDAGMYLDTLGIAV
                     RTGHHCTEPVMQRFNIPGTIRASFLFYNTMSEVDALVDGVKNAVKFLKR"
     gene            777..>1179
                     /locus_tag="PK134_06405"
     CDS             777..>1179
                     /locus_tag="PK134_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRB38515.1"
                     /translation="MPDHKAEKETMNFLEEMIDKEIHSGKIKNESLLTRFPPEPNGYL
                     HLGHASSICLNFGIAKRFGGKTNLRFDDTNPVTEDTEYVDSIKADIRWLGFEWANEFY
                     ASDYFETLYEFAIKLIHAGLAYVDDSTAEEIA"
     assembly_gap    1180..1204
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1205..2441
                     /gene="glnS"
                     /locus_tag="PK134_06410"
     CDS             <1205..2441
                     /gene="glnS"
                     /locus_tag="PK134_06410"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRB38516.1"
                     /translation="RSVEENIQLFESMRKGEFKEGEKTLRAKIDMAAPNMHLRDPLMY
                     RIKHAHHHRTGDKWCIYPMYDFAHGQSDSIENITHSICTLEFEVHKPLYNWFIEKLNI
                     YPSRQFEFARRNISYTVMSKRKLLQLVNEKIVDGWDDPRMPTISGVRRRGYTPESIRN
                     FAEVAGISRRENVTDFNLLESCLREHLNKIAVRTMAVINPLKVVLLNYEEHKTEILKA
                     ENNPEATEKTYRDIAFSREIFIEQDDFMEHASEGFFRLSIGGMVRLKHAYIIKCENVV
                     KNSDGTIKEIHCTYFPESKSGQDKSGIKVKSVIHWVNAANAVTAEVRLYDKLFTVEAP
                     DAQNEDFKKFINPASLQIFSNAKIEPNMLNYEKDERFQFLRLGYFVEDKYSTSQKRIY
                     NRAVTLKDDWVKEQKKQHK"
     gene            2443..2859
                     /locus_tag="PK134_06415"
     CDS             2443..2859
                     /locus_tag="PK134_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRB38517.1"
                     /translation="MTIAQIEEEIVSDFELFDDWSQKYEYIIEIGQQLPALDEKFKTD
                     VYKIKGCQSSVWLNASQKDGLLFFEADSDSVFVKGEIALLIKVLSGRTPEEIMSAELK
                     FIDAIGLRQHVAVTRANGLANMIKQIKAYALAHKER"
     gene            complement(2870..>3335)
                     /locus_tag="PK134_06420"
     CDS             complement(2870..>3335)
                     /locus_tag="PK134_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRB38518.1"
                     /translation="PHPDGQHILTACGDGTVRILNVNTLKEEQVIIAHTAGFSVNALC
                     LTPDNKFLLTGSRDARLNVFDVQQNFNLIENIAAHNYAIYDIAFNPSGKIMATASRDK
                     KIKIWNNDLTFITRIEEYPVGHKNSVNTLCWLSDTELITSGDDRNIMLWHLN"
BASE COUNT         1079 a          515 c          697 g         1009 t
ORIGIN      
        1 tatagtagtt gatgttgcac aggcagtagt tcatgaaccg ttgaacgtac aaaaattaga
       61 ttgtgatttt ctttgttttt catcacataa aatgttaggg ccaacaggaa caggtgttct
      121 ttatggtaaa gcaaaatggc tgaatgatat gccgccttat cgtggtggtg gtgatatgat
      181 taaaagtgtg agctttgaaa aaactattta caacgacatt ccttttcgct ttgaagcggg
      241 aacacctaat atcgctgatg taattgcatt tggaaaggca atagattatc tgaatagcat
      301 agaccgcatt gttgcactca attatgaaat gggtttagca aagaaagcaa gcagnnnnnn
      361 nnnncctcct tagtgctatt gaaggtgtta ggctaatagg tacagcagag aataaaacaa
      421 gtgtagtgtc ttttattatt gaaggtgtta atgccatgga tgcaggtatg tatctcgaca
      481 ctttaggtat tgcagtgcgc accggacatc attgtacaga acctgttatg caacgattta
      541 atataccggg aactattcgt gcatcgtttt tgttttataa caccatgtca gaggttgatg
      601 cattggttga tggagtgaag aatgctgtca agtttttgaa gaggtaattc tgaactattc
      661 tttaaccttc cttagctgtc agcgtcttga atatatttgt ttagcagtat tatcctgaaa
      721 aagttcctga attccatcga gcgttttctt tacctttgcg atccaaataa tacagtatgc
      781 cggatcataa agccgaaaaa gaaacaatga attttcttga agaaatgatt gataaggaaa
      841 ttcattcagg caaaataaag aacgaaagct tgcttacccg ttttccacct gagcccaacg
      901 gttatttaca tcttggtcat gcatcatcaa tttgtttgaa ttttggtatt gctaaacgat
      961 ttggcggaaa gactaatctc cgttttgatg atacaaaccc tgtaactgaa gatacagagt
     1021 atgtagatag tataaaggca gatattcgtt ggcttggttt tgaatgggct aatgaatttt
     1081 atgcaagtga ttattttgaa acactctatg agtttgccat aaaacttatt catgcaggat
     1141 tagcttatgt tgatgactct acagcagagg aaattgcatn nnnnnnnnnn nnnnnnnnnn
     1201 nnnntcgtag tgtagaagaa aatattcagc tttttgaaag tatgcgtaag ggagaattta
     1261 aagaagggga gaaaacattg cgtgcaaaaa ttgatatggc agcaccaaat atgcatttgc
     1321 gcgatccgct gatgtatcgt atcaaacatg ctcaccatca tcgtacaggt gacaaatggt
     1381 gcatttatcc gatgtatgat tttgcccatg gacagagtga ctcaatcgaa aatattactc
     1441 acagcatctg cacgcttgag tttgaagtac ataaaccact ttacaattgg tttatcgaaa
     1501 aactgaatat ttatcctagt cgtcagtttg aatttgcaag acggaacatc agttatactg
     1561 taatgagtaa gcgcaaacta ttgcagttgg ttaacgaaaa aattgttgat ggttgggacg
     1621 atccgcgaat gcctactatc agtggtgtgc gcagacgagg ttatactcct gaaagcattc
     1681 gtaattttgc agaagttgcc ggaatttcaa gacgtgagaa tgttacagat tttaatttac
     1741 ttgaatcgtg ccttcgcgaa catttgaata agattgcagt aagaacaatg gctgtcatta
     1801 atccattgaa agtagtgttg ttgaattatg aggagcataa aacagaaatt ctgaaagcag
     1861 aaaataatcc tgaagcaaca gaaaaaacat atcgagatat agcgttcagt cgcgaaatat
     1921 ttattgagca agatgatttt atggagcatg ccagcgaagg atttttcaga ctgtctattg
     1981 gtggcatggt tcgtttaaaa catgcttaca taataaagtg cgagaatgtg gttaagaata
     2041 gtgatggaac aattaaagag attcattgca cttattttcc cgagagtaaa agtggtcagg
     2101 ataaatcagg aataaaagta aagagtgtta ttcattgggt aaatgctgcc aatgcagtaa
     2161 ctgctgaagt gcgtttgtat gataaactgt ttaccgttga agcacccgat gcacaaaacg
     2221 aagattttaa aaaatttatc aatcctgcat cactgcaaat attcagtaat gcaaagattg
     2281 aacccaacat gctaaactat gaaaaggatg agcgttttca gtttttacgt ctaggatatt
     2341 ttgttgagga taaatacagc acttcacaaa aacggattta caacagagct gtaaccttaa
     2401 aagacgactg ggtgaaagaa cagaagaagc aacataagta acatgacaat agcacagata
     2461 gaagaagaaa ttgtttcaga ctttgaattg tttgatgatt ggtcacagaa gtatgaatat
     2521 atcattgaga taggtcagca gttgccggca cttgatgaga aatttaaaac ggatgtttat
     2581 aaaattaaag gttgccagag tagtgtatgg ctcaatgcaa gtcaaaagga tggacttctg
     2641 ttttttgagg ccgacagcga ttctgttttt gtaaaaggtg aaattgcctt gctgataaaa
     2701 gtattgagcg gacgcacacc ggaagagata atgtctgcag agttgaaatt tattgatgcc
     2761 ataggtctgc gtcaacatgt tgctgtcaca cgtgccaatg gtttggccaa tatgataaag
     2821 cagattaaag cttatgcatt agcacataaa gagcgttaat aatttacgat tagttaagat
     2881 gccatagcat tatatttctg tcatcaccgc tagtgattag ctctgtgtcc gaaagccaac
     2941 aaagcgtatt cacagaattt ttatgaccaa caggatactc ttcaatacga gtaataaaag
     3001 ttaaatcatt attccaaatt tttatttttt tatcgcgact ggctgttgcc attatttttc
     3061 ctgaagggtt aaatgcaata tcataaatag cataattatg agcagcaata ttttcgatta
     3121 gattaaaatt ttgttgtaca tcaaatacat ttagtcttgc atcacgtgag cctgtcagaa
     3181 gaaatttatt gtcaggtgtg aggcaaagtg catttacaga aaaacctgcc gtgtgagcaa
     3241 taataacttg ttcttctttt aaagtattta catttagtat tctgacagta ccatcaccac
     3301 aagctgtaag aatatgttgc ccatcaggat gaggt
//
LOCUS       DAORQT010000173         3328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5457_length_3328_cov_4.795906, whole genome shotgun sequence.
ACCESSION   DAORQT010000173 DAORQT010000000
VERSION     DAORQT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_3328_cov_4.795906"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..563
                     /locus_tag="PK134_06425"
                     /pseudo
     CDS             54..563
                     /locus_tag="PK134_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
     gene            576..1961
                     /locus_tag="PK134_06430"
     CDS             576..1961
                     /locus_tag="PK134_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRB38519.1"
                     /translation="MQKQKYFFTVLFWVLGLSLHAQTGTETPYSRYGIGELLNSGFAR
                     QSAMGGLGAAFQSSSAVNFSNPASYSSIRISTFETALRGELAQTSSSDLKQTKKGILL
                     NYLSLGFPVMKDNWGASFGLIPVSSTGYNLKEPGQVVNPNDTITTDFTKYYEGKGGLS
                     RFYIGNGFAPYSKTLDKYYASQHYKDLKEKGDTATINKEVRWRKIVKGFSAGFNASYL
                     FGSLNNIRRVKFSNSAFINTKITESTAYADFYFDYGIQYRYDFNEDNKLIIGISGAGG
                     TNIKGRRDVLWTLVSSSDFEANIDTVSLSENEKGKAFIPSFINAGFMFQQGEKWLMGA
                     EYATQQWSRYKAFNSNQNLKDSWRISAGAQYMPTNKLAYRAGIKVEQSYINLNDSRVN
                     DYSLSLGVGVPVRLTSTERRLFDNRTMLHFSIEGGQLGTTSNNLVKQQYVRLYVGITA
                     NELWFIKRKYD"
     gene            2064..3328
                     /gene="recJ"
                     /locus_tag="PK134_06435"
                     /pseudo
     CDS             2064..3328
                     /gene="recJ"
                     /locus_tag="PK134_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664021.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
BASE COUNT         1061 a          626 c          693 g          948 t
ORIGIN      
        1 taaaataaga aaaaccatta tcagcaacag aatttttaca ggtattttat ttaaaccata
       61 aaacagcatt acctatcgtt aatgattatg ccacgcctgc aacattaggc acagaccgtt
      121 tagccaatgc cgtgggtgca gcctttttat ttaaaaacaa aaattgtttg attttagatg
      181 caggcacttg tttaaagttt gacttaatta ccgctgatgc acattataaa ggaggagcaa
      241 tttctccggg attaaatatg cgctacaatg cattgcatga ctacacaggc aggcttcctt
      301 tggtaaagca gacagataat gttgcactta caggaaacac taccaaaacc tctattgcca
      361 gtggtgtgca gcatggcata gttgcagagg cagaacattt gataggtatt tataaaagac
      421 agtttaaagg tcttaaagtg attgttacag gtggcgatat ggcgtttttt gcagataggc
      481 tgaaaacaca catctttgca gccccggatt taacccttgt tgggttaaac agtattttaa
      541 attacaatct aagccggcag taaaacaagt tgataatgca gaaacaaaaa tattttttta
      601 cagtactttt ttgggtttta ggtctttcat tgcatgcaca aacgggaaca gaaacaccct
      661 attcgcgata tggtattggc gaattgctta acagtggttt tgcacgtcag tcggcaatgg
      721 gcggactggg agcagccttt cagagttcat ctgccgttaa cttcagcaat ccggcttcgt
      781 attcatctat tcgcataagc acttttgaaa ctgcattgcg tggcgagctt gcccaaacat
      841 catcatcaga tttaaaacag actaaaaaag gaattttgct caactatcta tctttaggtt
      901 ttccggtgat gaaagacaac tggggtgcca gcttcggatt aattcctgta agctctacag
      961 gctacaacct caaagaaccc ggccaggtgg ttaaccctaa cgataccatt accacagatt
     1021 tcaccaaata ctatgaaggg aagggcggat tgagtcgttt ttacataggt aacggttttg
     1081 caccctattc aaaaacgctc gataaatatt atgcatcgca acattataaa gacttaaaag
     1141 aaaaaggcga cacagcaacg attaataaag aagtgcgatg gagaaaaatt gtaaaaggtt
     1201 tttctgcagg ttttaatgca tcctatcttt ttggaagcct gaataacatt cgcagggtaa
     1261 agtttagcaa ttcagcattt atcaatacaa aaattacaga aagcactgcc tatgccgatt
     1321 tttattttga ttacggcatt caatatcgct atgatttcaa tgaagacaat aaacttatca
     1381 taggtatttc aggtgcagga ggaacaaaca tcaaaggcag acgtgatgtg ctttggacat
     1441 tggtttcctc atcagatttc gaagcaaata ttgacactgt ttctttatct gaaaacgaaa
     1501 aaggcaaagc atttattcct tcttttatca atgcaggatt tatgtttcag caaggtgaaa
     1561 agtggcttat gggtgctgaa tatgcaacac agcaatggag cagatacaaa gctttcaact
     1621 ccaatcaaaa tcttaaagac tcatggcgta tttctgcagg cgcacaatac atgcctacaa
     1681 ataaattagc ctaccgtgcc ggaataaaag ttgagcaatc gtacatcaac ctcaatgaca
     1741 gccgtgttaa tgactactca ctttctttag gtgtgggtgt gcctgtacgt ctgacttcaa
     1801 cagaaagaag attgtttgat aaccgcacca tgcttcattt tagcattgaa ggcggacagt
     1861 tgggtactac aagcaacaac ctagttaaac aacaatatgt gaggctttat gtaggtatca
     1921 cagccaacga gctttggttt atcaaacgga agtacgatta acggagcgtt aaatcagtgc
     1981 attaatcagc ttttttaagt tttagctcag ctcacactct atagctatag tgagggtgct
     2041 tatatttgct gacagaaaac aaaatgaaca agcgctggac actgctgaaa caggcagata
     2101 atgataagtc aataaagttt ggtaaagagt taaatattaa ccctgtgctt gccaacttgt
     2161 tgattcagcg cagtgtaaac acctatgacg aggcaaaaga gtttttcaga ccttcattgt
     2221 ctttgcttca cgatccattt ctgatgacag atatggataa agctattgcc cgtatagata
     2281 aagcaattgc taaaggtgaa aaggtattga tttatggtga ttatgatgtt gatggcacca
     2341 cggcagtagc gttggtttat cgttttttta aaaatcgctt tgccgagtgc aacttttata
     2401 ttcccgacag atatgctgaa ggatatggaa tttcttttca gggaatagat tgggcaaaag
     2461 aaaacggatt tacgttaatc atagcacttg actgcgggat aaaacaaagt atgcctcaga
     2521 catgggtata gattttatca tctgcgatca tcacagaccg ggagcagaaa ttccacaagc
     2581 tgctgctgtt ctcgacccaa aacgagtaga ttgtgcctat ccctgcaaag acctttcagg
     2641 ctgtggtgtt ggctttaaac tgcttcaggc atttgccaaa aaaaataata ttgaagagca
     2701 acaagtgctc gaccttattg accttgttgc cataagtatt gctgcagata tagttcctat
     2761 tcttggcgaa aacagaatct tagcctatta tggtcttgag cttatcaata aaaatccacg
     2821 tacaggaata aaagctattc ttgagttaaa tgctatcagg cgcgaactca acattaccga
     2881 tgtggtgttt attattgcac cacgcattaa tgcagcagga agaatggatc atggaaggtc
     2941 ggcagtgcaa ttgctcatca gcgattctgt tggcgatgct ataactgaga gcgaaaaaaa
     3001 tcaataaaaa caacattgac aggcgcgatg ccgacaaact cataacagaa catgccttgc
     3061 agcagattag ccgcgataat acttttgaaa accgaagaag taccgttgtt tacagtgcag
     3121 agtggaataa aggtgtgata ggcattgtag cttcacgact tacagaaaaa ttttatcgtc
     3181 ctacggtagt gcttaccgac agcaacgggc atattgcagg ctctgcacgc tctgtaaaag
     3241 attttgatgt gtataatgct attgaagcat gcagtgaata tttagatcag tttggcggac
     3301 acatgtatgc agccggactc acattaaa
//
LOCUS       DAORQT010000174         3321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5478_length_3321_cov_4.146663, whole genome shotgun sequence.
ACCESSION   DAORQT010000174 DAORQT010000000
VERSION     DAORQT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_3321_cov_4.146663"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..1941)
                     /locus_tag="PK134_06440"
     CDS             complement(490..1941)
                     /locus_tag="PK134_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38520.1"
                     /translation="MPLSVWSAPYLDYNERCKNAYQLIFELRFNQALDSLDAEKKEHP
                     DNLIPGYLYHYHLFIKTFIGEEQATFNELKHNHEIITGQIKAMPMLSPLRNFCLAECY
                     LMTALSKLKFKEYLTAALQVKKAYSLLTENQKKFPEFELNLKSLGVLHAFIGAVPENY
                     QWLVKIAGMDGSVSNGIKELEQSFMKTREDSDAFHFMNKEIAFTYIFTRNQLKPDPEW
                     VIKFLSGLKLGNEPLDLFFSTNIYYSTGRSAQIIPLIENYHADDEVYPIHYFQFLLGM
                     SKLDKLDFSSFSNFINYVKLHKGNSFIKAAYQKMAWIKLLQNDKAGYDAMITEVKNVC
                     NDFTDEYKQAQNELITHQEPNTYILKIRLLFDGGYYQEALKLLAKGSNEAKTAKDQLE
                     YKYRLARVLDKLEMTDRAKEFYLKTIDHGQDLKFYYAAASCNFLAQMYEKEKDYVLAE
                     KYYKKCLSLRDHEYQNSLDQKAKAGLNRIAGSR"
     gene            2130..3281
                     /gene="nhaA"
                     /locus_tag="PK134_06445"
     CDS             2130..3281
                     /gene="nhaA"
                     /locus_tag="PK134_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRB38521.1"
                     /translation="MIRGKIINPIRQLAEQGKLAGLLIIVLTLVSLLLSNLSATSEYA
                     GLWHSHIHLLGYDVSVLHIINDGLMVVFFLMVGMEIKRELYVGELANRRNAMLPVIAA
                     FGGMLAPGIIYAAFNISDTHLLHGWAIPTATDIAFSLGVLSMLGNRVPFALKVFLTAL
                     AIIDDLGAIIIIALFYTEPENFHLSFLVSSLLIVGALMFLSNKGFFNLFLWIIAGIVV
                     WYFMLLSGVHTTIAGVLIAFTIPLNKLTSFENKLHLPVNYFILPVFALANTAIVLSID
                     NVSGLFSTVGLGVSLGLLIGKPLGITLASWIAVKNKWCSLPGKINLKLIFGAGLTAGI
                     GFTMSIFIASLAFKEPLLLDTAKIAIIAGSVLSGLAGVLFLKSNLKAKA"
BASE COUNT         1029 a          598 c          519 g         1175 t
ORIGIN      
        1 tatttaaagt aatgattcta tcaattaacc ataataattt cattatcaac gtattaatca
       61 acttatacga ttaataatta aacattgaaa acattacttt aattagatgt ctgatatttt
      121 attgcatcgg tgttataaat aatacttttt tacttgcaat ctaatgcgct cgtaaaatca
      181 tctaaaaaca aattttcctg ataggggact tttaagttta ttaatctgga acttttttac
      241 tcacttcaga aatatagaat tattcagttt ttcaaattta aatttttgca acaggtttac
      301 taaaatgaat aagacattgc tctgaaattt ttaaatgtac aaaaccggtt aagaatggct
      361 tatgtatcat acaaatttga actatattct tagccataac attgtgcgga ttcctaatga
      421 ttcttaccct ggccgaccgg caattcaatc ttataattaa tattatgtta aatagtaatt
      481 atttgaatat tacctgctac cagcaattct gtttaacccg gccttagcct tttggtctaa
      541 actgttctgg tactcatggt ctcgtaaact aaggcacttc ttatagtatt tttcagctaa
      601 aacataatcc ttctctttct catacatctg tgctagaaaa ttacaactgg cagctgcata
      661 ataaaatttc aaatcctgcc cgtgatcaat agttttcaga taaaattctt tggcccgatc
      721 agtcatttcc aacttgtcca acacccttgc cagcctatat ttatactcca actgatcttt
      781 agctgtttta gcttcgtttg aacctttagc caacaatttt aacgcttcct gataatagcc
      841 tccatcaaat aatagcctga tttttaagat ataagtattt ggctcttgat gcgtaattaa
      901 ttcattttgc gcctgtttat actcatcagt aaaatcatta catacgtttt ttacttcagt
      961 aatcattgca tcatatccgg ccttgtcatt ttgtaatagc tttatccatg ccattttttg
     1021 atatgcagcc tttataaagc tatttccttt atgcaattta acataattaa taaaatttga
     1081 gaatgatgaa aagtctaatt tatcaagctt agacattcct aaaagaaatt gaaagtaatg
     1141 tatcggataa acctcatcat ctgcatgata attttctatc agtggaatta tctgcgcact
     1201 acgccctgta gaataataaa tgttagtaga aaaaaacaag tccaaaggct cgtttcccaa
     1261 tttcaatcct gacaaaaact tgattaccca ctcaggatca ggtttcagtt gattacgtgt
     1321 aaaaatataa gtaaaggcaa tttccttatt cataaaatga aaagcatcac tgtcttctct
     1381 tgtcttcata aacgattgct ccaactcttt tattccattg gaaacgctac catccatacc
     1441 ggcaattttc actaaccatt gatagttttc aggcactgca ccaataaatg catgcagcac
     1501 accaagactc tttagattca actcaaattc aggaaacttt ttttgattct ctgtaagcaa
     1561 actatatgct ttctttactt gcaaagctgc agtcagatat tccttgaatt tcagtttcga
     1621 caatgctgtc atcagataac attctgcaag acaaaaattt cgtaatggcg aaagcattgg
     1681 cattgcctta atctgtcctg ttataatttc atggttatgt tttagctcat taaaagtagc
     1741 ctgctcctcc cctataaatg tttttatgaa taaatgataa tgataaagat atcccggaat
     1801 aagattgtcg ggatgttctt tcttctctgc atcaagacta tctaaagcct gattaaaacg
     1861 caattcaaaa ataagctgat aggcattctt acatctttca ttatagtcaa gatagggtgc
     1921 tgaccataca cttaatggca tactaaaaaa caaaaaatat aaaattatat accgaagcct
     1981 catcagtctt aaaaaactat cgtgttgtgc aataatacaa agaccaaacg ataaaatgaa
     2041 tcaaaaatat attgatacta gcaatgaggc tatgttgaat acagtattga atgaacaaaa
     2101 aaactctcta taatttttag ttttgtaaaa tgattagagg taaaatcata aatccaatac
     2161 gtcagcttgc agagcaaggc aaattagccg gccttttgat tattgttctc acgctggtgt
     2221 cattgcttct aagtaattta tcggcaacat cagaatatgc aggcctatgg catagccata
     2281 tacatttatt gggttacgat gtaagtgtgc tgcacataat taatgacgga ttaatggtgg
     2341 tattttttct gatggttggc atggaaataa aacgagagct gtatgtgggc gaacttgcca
     2401 ataggcgcaa tgcaatgttg ccggtcatag cagcttttgg tggaatgctt gctcccggaa
     2461 ttatctatgc agcatttaac atcagtgata ctcatctgct tcatggttgg gcaattccca
     2521 ctgcaacaga cattgcattt tctcttggtg tactatctat gctgggtaac agagtgccgt
     2581 ttgcacttaa agttttttta actgcattag caattattga tgaccttggc gctattatca
     2641 tcattgcatt attctacacc gaacccgaga attttcatct gtctttttta gtcagtagtt
     2701 tactcattgt tggcgcatta atgtttcttt caaacaaagg tttctttaat ttatttcttt
     2761 ggattattgc aggtattgta gtctggtatt ttatgctgct ttcaggagtt catacaacaa
     2821 ttgcaggtgt actgatagca tttacaattc cattaaacaa actcacatca ttcgaaaaca
     2881 aacttcacct gcctgttaac tattttattc tccctgtttt tgcattagct aatactgcta
     2941 ttgttttatc tatagataat gtttccggat tgttttctac tgtgggatta ggggtaagtt
     3001 tgggtttgct aattggaaag ccattaggca ttacattagc ttcatggatt gcagtaaaaa
     3061 acaaatggtg ttcactgcca ggaaaaatta atttaaagtt gatttttgga gcaggtttaa
     3121 cagcaggcat aggttttacg atgtctattt ttattgcttc gcttgctttt aaagaaccct
     3181 tactactcga cactgcaaaa attgcaatta ttgccggttc tgttttatcg ggtttggcag
     3241 gtgttttatt tttaaaatca aatttgaaag caaaagcatg atttattttt aatcatcctt
     3301 cggatagcgc ttgcgctgtt t
//
LOCUS       DAORQT010000175         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5496_length_3314_cov_8.007364, whole genome shotgun sequence.
ACCESSION   DAORQT010000175 DAORQT010000000
VERSION     DAORQT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5496_length_3314_cov_8.007364"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="hemW"
                     /locus_tag="PK134_06450"
     CDS             <1..967
                     /gene="hemW"
                     /locus_tag="PK134_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRB38522.1"
                     /translation="YFGGGTPSLLSAAEINSIIGKLDKVFKLDVKEITLEANPDNLSE
                     KYLKELKSTAVNRLSIGVQSFEEKRLLWMNRSHTAKQAIFAIESALQMGFNVSADLIF
                     ALPEMSTDEWQRQLQKMISLKPHHISAYSLTLEERTVYHHQVAKKKVKELDDVNSEEQ
                     FLQAHQLLSNAGYHHYEISNYASAGHVALHNSSYWNGKLYLGLGPSAHSFNGKMRQWN
                     VSNNTAYIKALSAGKLSFESEVLTEVDRYNEYVMTGLRTDKDIEEKQWNELMPSLYKE
                     SLQKEMNELVNQQFLVRDASRIFIPLQHWYKADQLISRLFATENS"
     gene            973..1725
                     /locus_tag="PK134_06455"
     CDS             973..1725
                     /locus_tag="PK134_06455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRB38523.1"
                     /translation="MIAHLKIGDEEYSAKLNEPIDISIPLHSGNDNVNAWYAPPPEIV
                     PVRMDSWVGEVAQGASVNFRNVFFNPHAHGTHTECVGHITKEWVSLNKVLKQFFFSAK
                     LITVLPMIVDGDMIITKEDVVQAWKNCGAEALVIRTLPNDNSKQSRHYSNTNPPYLHH
                     EAAAWMAEKNIKHLLLDLPSVDREQDGGKLLAHNAFWSTGKNIRYDCTISEMIFVPDH
                     IADGLYLLNIQITALENDASPSKPLLFQLTKK"
     gene            1737..2321
                     /locus_tag="PK134_06460"
     CDS             1737..2321
                     /locus_tag="PK134_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRB38524.1"
                     /translation="MKKLLNYFFQGLLVVVPIAVTILVIYKIITWIDNLLPFQIPVKL
                     PGIGEVSIPGLGILTIVVAVTAFGYLAKFFVANPFFVLLERMLERTPLLKIIYTSVKD
                     LIEAFVGEKKRFNQPVLVTVNKVSGVQRIGFITQKDLSVLGISSNKMAVYLPFSYGFN
                     GQLVIVPAENVEKIDASGTEMMKFVISGGVTEVD"
     gene            2365..>3314
                     /locus_tag="PK134_06465"
     CDS             2365..>3314
                     /locus_tag="PK134_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38525.1"
                     /translation="MLILMGSGVCAQDYFIPLNREWNLRYEPFLQTTKSNNVHTSIKP
                     YRHDEIIAVANIDSVDTANFPISRFSKTLVGRKIFKEHLVQIKDDDFLIHADFIFEGR
                     IGKDLPETETLFTNSRGFWLGGTAGKRFSFSTTFFENQARFPSYIDTFVTRYNIIPGE
                     GRIKKQYGSYDYSNVTGTISYTINRHFNVQFGHDKNFIGDGYRSLLLSDNANSYPFLK
                     ISTTAWKVKYVNLFTVLTDLQFPAPNDIAFKKKYASFHYLDLNIGKHASFGILESVVW
                     SGDSARGQGFDINYMNPFVFIRPVEFSIGSPDNMMLGFNL"
BASE COUNT         1049 a          563 c          701 g         1001 t
ORIGIN      
        1 ctattttggt ggaggaacac catcattact ttcagcagca gagattaaca gtattatcgg
       61 aaagctagat aaagtgttta aacttgatgt taaagaaata actttagaag ccaaccccga
      121 taatctttct gaaaaatatc tgaaagaatt gaagtcaaca gcagtgaaca gactgagcat
      181 tggtgtgcag tcgtttgaag aaaagaggct gctgtggatg aacagaagtc atacagcaaa
      241 acaggcaata tttgctattg agtctgcgct gcaaatggga tttaatgtta gtgccgattt
      301 aatttttgcc ttacctgaaa tgtcaacaga tgaatggcaa aggcagttgc agaagatgat
      361 aagtttaaaa ccacatcata tttctgccta tagtttaaca cttgaagaaa gaacggttta
      421 tcatcatcag gttgcgaaga aaaaagtcaa ggagttagat gatgttaatt cggaagagca
      481 gtttttgcag gcacatcaac tgttgagcaa tgcaggatat catcactatg aaatatcgaa
      541 ctacgcaagt gccggacacg ttgctttgca caacagcagc tattggaacg gtaaacttta
      601 tttaggtcta ggaccatcgg ctcatagttt taacggaaaa atgcggcaat ggaatgtcag
      661 caacaacact gcgtatatta aagcattgag tgcaggaaaa ctttcttttg aaagtgaagt
      721 gctgacagag gttgaccgat acaatgaata tgtgatgaca ggcctgagaa cagataaaga
      781 cattgaagaa aaacaatgga atgaattaat gccgtcattg tataaagaga gtttgcagaa
      841 agaaatgaat gagttggtta atcagcaatt tttagttcgt gatgcttctc gtatatttat
      901 acccttgcag cattggtata aagcagatca gttaatcagc aggttgtttg caacagaaaa
      961 tagttaaaag aaatgattgc acatttaaaa ataggagacg aagagtattc agctaagctc
     1021 aatgagccaa tagatatttc tattccattg cactccggca atgataatgt taatgcatgg
     1081 tatgcacctc caccggaaat tgttcctgtg cgcatggata gttgggtagg cgaagtggcg
     1141 caaggtgcat cagtaaattt caggaatgtg ttttttaacc ctcatgcaca tggtacacat
     1201 acagagtgtg taggacatat caccaaggag tgggtttcgt taaataaagt attgaaacaa
     1261 tttttctttt ctgcaaagct gataacggtg ttgccaatga ttgttgatgg cgatatgatt
     1321 attaccaagg aagatgtagt tcaggcatgg aaaaactgtg gtgccgaagc attagtcatt
     1381 cgtacattac ctaatgacaa cagcaaacaa agcagacatt attcaaatac caatccgccc
     1441 tatctgcatc atgaagcagc agcgtggatg gcagaaaaga atatcaaaca tctgctactc
     1501 gaccttccct ctgttgaccg cgaacaggat ggaggaaagc tgcttgcaca caatgcattt
     1561 tggagtacag gaaaaaatat tcgttatgat tgcactattt cggaaatgat ttttgtgccc
     1621 gaccatattg ccgatggact atatttgctc aatattcaga ttactgcatt ggagaatgat
     1681 gcaagccctt ccaaaccact attgtttcag ttgactaaaa aataaaatgt gtagagatga
     1741 aaaaactgct taattatttc tttcagggat tgttggttgt tgtacctatt gctgttacca
     1801 ttttagtaat ctataaaatc ataacctgga ttgacaacct gttgcctttt cagattccgg
     1861 ttaagttgcc cggcattggc gaagtgagca ttcccggact tggcattctt accattgtag
     1921 ttgctgtaac tgcttttggt tacttagcta aattttttgt tgctaatcct ttttttgttt
     1981 tgctcgaacg catgttggag cgaacaccgt tgttaaaaat tatttacact tcagtaaaag
     2041 atctgataga agcatttgtt ggagagaaaa aacgatttaa tcagcctgtg ttggtaacgg
     2101 taaataaagt gtcgggagtg cagcgcattg gtttcatcac acaaaaagat ttatcggtgc
     2161 ttggcatcag cagtaataaa atggctgttt atcttccttt ctcctatggt tttaacgggc
     2221 agttggtgat agtgcctgca gagaatgtag agaagattga tgcatcagga acagagatga
     2281 tgaagtttgt aatttcagga ggtgttacag aagtagatta aacaaagaac tatgaatatt
     2341 tttcggtttg cattatttgc agcaatgtta attttaatgg ggtcaggtgt ttgtgcacag
     2401 gattatttta ttccattaaa tagagagtgg aacctgcgct atgagccttt tcttcagaca
     2461 acaaaaagca ataatgttca cacttcaata aagccataca gacatgatga aattattgct
     2521 gttgcaaaca ttgattctgt tgacacagca aatttcccga taagtcgttt ttcaaaaacc
     2581 ttagtcggac gaaaaatttt taaagagcat ttagtacaaa tcaaggatga tgactttctg
     2641 atacatgccg attttatttt tgaaggacgc attggcaaag accttccgga aacagaaaca
     2701 ttattcacta attcaagagg attctggctt ggcggaacgg caggaaaaag attttctttc
     2761 tctactacat ttttcgaaaa tcaggcaagg tttccgtcat acattgatac gtttgttaca
     2821 cgctacaaca ttattcccgg tgaggggcgc ataaaaaaac aatatggcag ctatgattat
     2881 tccaacgtta caggaactat atcatacacc atcaacagac attttaatgt gcagtttgga
     2941 cacgataaaa attttattgg cgatggttat cgctctttgc ttttgtctga taatgccaac
     3001 agctatcctt ttctgaaaat ttcaactacg gcatggaagg ttaagtatgt aaatttgttt
     3061 accgtactca ccgaccttca gtttcctgca cctaatgaca ttgcttttaa aaagaaatat
     3121 gcatcattcc attatttaga ccttaacatc ggcaaacatg catcgttcgg aattttagaa
     3181 agtgttgtct ggtcgggcga ctcggcacgt gggcagggtt ttgacattaa ctacatgaat
     3241 ccttttgtat ttattcgtcc tgtagagttt tcaataggtt cgccagataa tatgatgctg
     3301 ggttttaatc tgaa
//
LOCUS       DAORQT010000176         3312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5504_length_3312_cov_5.946270, whole genome shotgun sequence.
ACCESSION   DAORQT010000176 DAORQT010000000
VERSION     DAORQT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5504_length_3312_cov_5.946270"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PK134_06470"
     CDS             complement(<1..605)
                     /locus_tag="PK134_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRB38526.1"
                     /translation="MNNDAPFQFLPGFTHLYHEYMEENIFIAYSEKLMAYMPLRFFSS
                     RFFKLAQILHAPIKNNIELNPDEQLEFFNELISHLNQNNSCERLVQPHPYGILASVPA
                     NSRFCEFGTYIIDLQSQTLEEIFQKFHPKYQKAIHHSEKNGAVVKFGQEVIEDFYLCY
                     TDTMKRINMPSEELQFFKSYYNYLGSDNVTAGVVYDHDNPIG"
     gene            complement(660..1787)
                     /gene="wecB"
                     /locus_tag="PK134_06475"
     CDS             complement(660..1787)
                     /gene="wecB"
                     /locus_tag="PK134_06475"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRB38527.1"
                     /translation="MKKILIVVGTRPNFIKVTQFKKVNADLGMPFDIKIVHTGQHYDS
                     KMADIFFEQFNLTPDFWLNIPAGSPNSQMAEIMLRLEKVIQENKPDLLMVVGDVNSTF
                     AAALTANKMDLKIAHVESGLRSFDRTMPEEINRLLTDEITDYFFITEDSGYKNLLKEG
                     KHEKQMFFVGNTMIDTLVAFDEKIQQSNILETLNIKSKEFVLMTMHRPATVDHKDGLK
                     QLFSIIELITKNHQLVFPIHPRTLHRIEEFGMKSFITENKKLILTEPLDYLAFQKLIS
                     DCAYIVTDSGGIQEESTFRQIPCLTLRANTERPCTVDIGTNELVPFSQEKISERIDAI
                     VHNKYKKGVIPPLWDGQSTKRILEACMKFLSSESHASALLK"
     gene            complement(1842..2984)
                     /locus_tag="PK134_06480"
     CDS             complement(1842..2984)
                     /locus_tag="PK134_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRB38528.1"
                     /translation="MIKRLISHIRLRYLNLLSQSTAENFSDEQRLLMALEWLKQTLLP
                     NGGSAAKYSMMFNKWLPAYPETTAFWINTLIYLKNNKPELLQEVFNDRPVIDELIQWL
                     LSTQRKDGTFPGSYGDFKNQPPRVFNNGQIIMALTDYYQQSQKKEVLEAAIQSADWLL
                     KVQDSDGAWREFTLHQLSSNTRTAWALIKLGQHLNEPKYISAGINNINFALKQINNHD
                     YFCDNGFDENEIPTTHTIGYALRGVLGAGIDLQKKEWTEIVERSYKHIVALTKQNGFL
                     AGELDEEWTSDNAFCCLTGNCQLSVIGFVLFEQTAEIQYLETANLLLEYVKSKQLISH
                     SPMVNGGISGSWPVNGGYSAYDIPNWAAKFFADAMLLQMKHKTMKH"
     gene            complement(2994..>3312)
                     /locus_tag="PK134_06485"
     CDS             complement(2994..>3312)
                     /locus_tag="PK134_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB38529.1"
                     /translation="PKGVESEVGERGSRLSGGQKQRIGIARSLYRNAQILVFDEATSA
                     LDTQTEREVSEAIDSLSDTNKTIFIIAHRVTTLKNCDRIYELKNGEIAGVYSYSELIE
                     KVI"
BASE COUNT          970 a          586 c          590 g         1166 t
ORIGIN      
        1 cctattggat tgtcatgatc atatacaaca cctgctgtaa cattatcgct gccaagataa
       61 ttataataag atttaaaaaa ctgtagctct tctgaaggca tgtttattcg tttcatagta
      121 tcagtataac ataaataaaa atcttcaatc acttcttgtc cgaattttac aacagctcca
      181 tttttttctg aatgatgaat agctttttga tattttggat gaaacttctg aaagatttcc
      241 tccaatgttt gtgattgaag gtctatgata taggtgccaa attcacaaaa acgcgaattg
      301 gcaggtacag aggcaagaat tccataagga tggggttgaa ccaatcgttc gcaggaattg
      361 ttttgattta agtgcgatat taattcgtta aaaaattcaa gttgttcatc cggattaagt
      421 tcaatattat ttttaattgg cgcatgtaat atttgtgcaa gtttgaaaaa tcgtgaagag
      481 aaaaatcgca atggcatata tgccatcaat ttttctgagt atgcaataaa aatattttct
      541 tccatatact catgatacaa atgagtaaac ccgggaagaa actgaaacgg tgcgtcatta
      601 ttcattgcgg ccaataatga cttgtctttt ttctgtaggt ggttgagtag taacatagtt
      661 tatttcaata gggcagaggc atgactttcg cttgaaagaa atttcataca agcttctaaa
      721 attcgtttgg tggattgtcc atcccataat ggaggaataa ctcctttttt atatttgtta
      781 tgtacaattg catcaatgcg ttcggaaatt ttttcctgac tgaatggtac caattcatta
      841 gttccgatat caactgtaca tggtcgttct gtattggctc ttaatgtaag acatggtatt
      901 tgtctgaatg tactttcttc ctgaatacct ccgctgtctg ttacaatata agcacagtct
      961 gaaataagtt tctgaaatgc cagatagtct aaaggctctg ttaatattag ttttttgttt
     1021 tccgtgataa atgatttcat tccaaactct tcgattctgt gcagtgtacg tgggtgaatc
     1081 ggaaatacaa gttgatgatt ttttgttata agttcaatta ttgaaaacaa ttgcttcaga
     1141 ccatctttat gatcaaccgt tgccggacga tgcatagtca tcaggacaaa ctctttactt
     1201 ttaatattta aagtttcaag aatattactt tgttgaattt tttcatcaaa ggctacaagt
     1261 gtatctatca tggtgtttcc aacaaagaac atttgctttt catgcttgcc ttccttgaga
     1321 agatttttat agccactgtc ttcggtaata aaaaagtaat cggttatctc atcagttaat
     1381 aaacggttta tttcttcagg cattgttctg tcaaaacttc tcaggccact ttcaacgtga
     1441 gcaattttta aatccatttt attggctgtt aatgctgcag caaaagttga atttacatcg
     1501 ccaacaacca ttaataagtc aggtttgttt tcctgaataa ctttctctaa gcgaagcatt
     1561 atctcagcca tttgagaatt aggactgcct gccggaatgt taagccagaa gtcaggtgtt
     1621 aaattaaatt gctcaaagaa aatgtcagcc atcttactat cgtagtgctg gccagtatga
     1681 actattttaa tatcgaaagg catacctaag tcggcattca cctttttaaa ctgtgttact
     1741 ttaataaaat ttggtctggt cccaactaca attaaaatct ttttcattct caaaatctat
     1801 tgtcttttta tacgtgaagg taggtataaa gtttaaaata tctaatgttt catagtttta
     1861 tgtttcattt gcaagagcat ggcatcagcg aaaaattttg ctgcccaatt tggtatatca
     1921 taagcagaat aaccaccatt tacaggccat gatcctgaaa tgccgccatt taccattggc
     1981 gaatgtgaaa ttaattgttt gcttttaacg tattcaagca aaagattcgc tgtttctaag
     2041 tattgaattt cggctgtttg ttcaaataat acaaatccga taacacttaa ctgacagttg
     2101 cctgtcagac agcagaatgc attgtcgctt gtccattctt cgtctaattc tccagccaga
     2161 aagccatttt gtttagtaag tgcaacgatg tgtttatagg agcgctcaac aatttcagtc
     2221 cattctttct tttgaagatc aatacctgca cctaatacac ctcgcaatgc ataaccgata
     2281 gtgtgtgtgg ttggtatttc attttcatca aaaccattgt cacaaaagta gtcgtgatta
     2341 ttaatctgtt ttaaggcgaa gttgatatta ttaattcctg ctgaaatata ttttggttca
     2401 tttaaatgtt ggccaagttt gattaaagcc catgctgttc tggtgtttga gctaagttga
     2461 tgtagcgtaa attcacgcca tgcaccatca gaatcttgca ctttcaaaag ccaatcagca
     2521 ctttgaatag cagcctcaag gacttccttt ttctgacttt gttgataata gtcagtaagc
     2581 gccataatta tttgaccatt attaaaaact ctgggaggtt ggtttttaaa atctccatac
     2641 gatccgggaa atgtaccgtc tttacgttga gtagataaaa gccattgaat taactcatca
     2701 ataacaggtc tatcattaaa tacttcctgt aataattccg gtttgttatt tttcagatag
     2761 ataagagtgt ttatccaaaa agcagtggtt tcagggtagg cgggtaacca tttgttaaac
     2821 atcattgaat acttggcggc acttcctcca tttggtaaaa gtgtttgctt gagccattca
     2881 agtgccataa gtaatctttg ttcatcagaa aaattttcgg ctgttgattg cgataataaa
     2941 ttgagatatc gcaaccttat gtgtgaaatc agccgtttga tcatcgttaa aaattaaatt
     3001 actttttcaa ttaactcgct gtaggaatat actcctgcaa tttccccatt ttttagttcg
     3061 taaatgcggt cgcagttttt tagtgtagtc acgcggtgag cgatgataaa gattgtttta
     3121 ttggtatctg aaagtgagtc aatggcctca cttacttcgc gttctgtttg tgtgtccaat
     3181 gcactggttg cctcatcaaa aacaagtatc tgtgcgtttc tataaagcga gcgtgcgatg
     3241 ccaatccttt gtttttgccc accagatagt cttgatcctc tttctccaac ttctgactct
     3301 actcctttgg gt
//
LOCUS       DAORQT010000177         3294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5550_length_3294_cov_8.152825, whole genome shotgun sequence.
ACCESSION   DAORQT010000177 DAORQT010000000
VERSION     DAORQT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_3294_cov_8.152825"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PK134_06490"
     CDS             complement(<1..1141)
                     /locus_tag="PK134_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRB38530.1"
                     /translation="MNKLKIGILFGGSSREREVSFAGGRTVYDNLDKTLFEPIPLFVD
                     SFGNLLILDWQFIYKGSIRDFYPPSEFLPETTRGFQIYAENLGTLTHQEQIRLTMKLG
                     TRIEFAQLHDVIDFAFLCLHGSDGEDGRIQGMLEYYRITYSGSGILSSAIGMNKVIQK
                     ELMKKSGFNVPEVAVINRIEWLASPNRKSFIKNLKSVGFPCVVKAANQGSSIGISVLK
                     NNDVEEFIQAVNKSFFIREITRTEWNSMTFDTKTEWIKKVSDIREGIGLPAKINDRTI
                     YHPEELLGVLIQLFGEMEDVVSIAAIEAETEVIIESFISGKEFSCIVITNENEKPVAL
                     PPTEIRKGNELFDYRAKYLPGLSRKVTPIHLPEVQIETIRRECERL"
     gene            complement(1328..2092)
                     /locus_tag="PK134_06495"
     CDS             complement(1328..2092)
                     /locus_tag="PK134_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRB38531.1"
                     /translation="MNHHELKLYLDDLFLRYNRSDFIADDPISIPHKFSDSGDIEISA
                     FLTSLISWGQRKTILKNAQTLMEMMDHTPKSFIINASNNDYIPFKKFVHRTYNGEDCI
                     YIIKAIKNIYAEYGSLGNCLTSFVSNNPMPVAMSLFRQKLFSQPCQPRTTKHLGDPLK
                     NSSCKRFNMFLRWMIRKDRAGVDFGIWEISPSLLYCPLDVHTGRTARLLGLLKRQQDD
                     WKAVEELTINLRQFDKSDPAKYDFALFGAGVNGLLK"
     gene            complement(2105..>3294)
                     /gene="der"
                     /locus_tag="PK134_06500"
     CDS             complement(2105..>3294)
                     /gene="der"
                     /locus_tag="PK134_06500"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRB38532.1"
                     /translation="GEWTGRSFSVIDTGGYVSGSEDIFESEIRKQVKLAIEEADIILF
                     VTDAREGLNDFDEDIAQLLRNSNKKTFLVVNKVDSPKQLNDAVEFYALGLGDYYCISS
                     SNGSGTGELLDAVVKVFKPDDEEEDELPRITIIGQPNVGKSSLMNMLTGVERSIVTPI
                     AGTTRNSINIRYSNFGFDFILVDTAGLRRKAKVKEDLEFYSVMRSIRAIEQSDVCLFM
                     IDAEKGFTAQDANIFHLIQTNHKGVVFLVNKWDLIDKENHSTKKFTEEIKAVIAPFTD
                     VPIIFTSVLNKQRIFKALETAIDVFKNRTQHIPTSQLNKKILPFIENYPPPSVKGKLI
                     NIKYITQVPGPYPRFVFFSSNPQYIQTSYRRFLENKLRENFNLSGVPVAISFRSKDSG
                     KEV"
BASE COUNT          985 a          619 c          578 g         1112 t
ORIGIN      
        1 acagacgttc acactctctt cttattgttt caatttgtac ttcgggcagg tgaataggtg
       61 ttacttttct tgataaaccg ggcaggtatt tagctcggta gtcaaacaac tcattgcctt
      121 tgcgtatttc tgttggcggt aatgcaacag gcttttcgtt ttcatttgta attacgatgc
      181 atgaaaactc ttttcctgaa atgaatgatt caataataac ttctgtttca gcttcaatag
      241 cagcaataga aacaacgtct tccatttcgc caaaaagttg tatcagaact cccaataatt
      301 cttccgggtg atagattgtt ctatcattaa tttttgctgg taaaccaata ccctcgcgaa
      361 tatcagatac tttctttatc cattctgttt ttgtatcaaa agtcatgctg ttccattctg
      421 tccgagtgat ttcgcggata aaaaatgatt tgttgacagc ttgtataaat tcttctacat
      481 cattattctt caatactgat attccgatac tcgaaccctg attagcagct ttaactacac
      541 aaggaaatcc aacagatttt aaatttttaa taaaagattt tcggtttggt gacgcaagcc
      601 attctattcg gtttatgaca gcaacttcag gaacattaaa acctgatttt ttcatcagtt
      661 ctttctgaat aactttattc attcctattg cagaagaaag aatacctgaa cctgagtaag
      721 tgattcggta atattccaac attccttgta tgcgtccatc ttcgccatca gagccatgca
      781 gacataagaa tgcaaaatca attacatcat gtaactgcgc aaactctatg cgcgttccta
      841 acttcatcgt tagtcttatt tgttcctgat gagtgagcgt tccaagattt tctgcataaa
      901 tctgaaaacc acgtgttgtt tcgggtaaaa attctgatgg aggataaaaa tcacgaatgc
      961 tacccttgta tataaattgc cagtcgagaa tcagtaggtt gccaaaactg tcaacaaaaa
     1021 gaggaatagg ttcaaatagt gttttgtcga ggttgtcata tacggttctg cctccggcaa
     1081 aggacacctc gcgttcgcgt gatgagccgc caaaaagaat tcctattttt agtttgttca
     1141 tataaatttt aagtcatgaa atgattgaac aaagttaaca gattaataga gcagcataca
     1201 tgctataaga gtaagtattc actatttttt taacatttat tttgaaagca gtttttatta
     1261 aatacacgtt agaaagattg ttttttaaat tggtttttgc tcaataaaaa cgactgatat
     1321 ccagagctta ttttaacaac ccattgacac ctgcaccaaa aagtgcaaag tcgtattttg
     1381 cagggtcgct tttatcaaat tgtcgaagat ttatagtcag ttcttcaacg gctttccaat
     1441 catcttgctg tcttttcagc agcccaagta gtcgtgctgt tcgaccggta tgtacatcta
     1501 aaggacaata aagtaatgag ggtgatattt cccaaattcc aaagtcaaca ccggcacggt
     1561 ctttccttat catccaacgt agaaacatat tgaatctttt gcaacttgaa ttttttaatg
     1621 ggtcgcctaa atgcttggtg gtgcgtggtt ggcagggttg ggagaatagt ttctgcctga
     1681 ataaactcat agctacaggc atgggattgt tgcttacaaa agatgttaag caatttccta
     1741 acgatccgta ttcagcataa atatttttaa tagctttaat aatgtagata caatcttctc
     1801 cattataagt gcggtgcaca aattttttaa aagggatata atcgttgttt gatgcattta
     1861 tgataaatga ttttggagta tgatccatca tttccataag tgtttgcgca tttttcagaa
     1921 tggtttttcg ctgcccccat gagataagag aggtcagaaa ggcgctgatt tcaatatcac
     1981 cactgtcaga gaacttatga gggatagaaa ttggatcatc agcaataaaa tcacttctgt
     2041 tgtatcgcag aaataaatcg tctaaataca gtttgagctc atgatgattc atgattttct
     2101 gttttcagac ttccttgcca ctgtccttac ttctaaaaga aattgcaaca ggaacaccac
     2161 tcagattgaa attttcgcga agtttatttt caagaaacct tctgtacgaa gtttgtatgt
     2221 attgcggatt gctactaaaa aagacaaaac gtggatatgg tccgggcacc tgagtaatat
     2281 acttaatatt gatgagttta cctttgacgg acggtggagg ataattttct atgaaaggta
     2341 gtattttttt gttaagctgc gatgtcggaa tatgttgcgt tctgtttttg aaaacatcaa
     2401 tagcagtttc aagtgcttta aaaatacgtt gcttgtttag taccgaagta aagattattg
     2461 gtacatcagt aaatggagca atgacagctt ttatttcttc tgtaaatttc tttgttgaat
     2521 ggttttcttt gtcaattaaa tcccatttgt tgactaagaa cacaacaccc ttgtgattgg
     2581 tttgaatgag gtgaaaaata tttgcatcct gtgctgtgaa tcctttttct gcatctatca
     2641 taaacagaca cacatcactt tgctcaatgg ctcttatgct gcgcattaca gagtagaatt
     2701 ccaaatcttc tttcactttg gctttccttc tcaatcctgc agtgtcaact aaaataaaat
     2761 caaaaccaaa atttgaataa cgaatattta tactatttct ggtagttcct gcaataggtg
     2821 taacgatact gcgttccaca ccggtaagca tattcattaa agaagatttg ccgacattag
     2881 gctgaccaat aattgtgatt cttggcaact catcttcttc ctcatcatca ggcttaaaaa
     2941 ctttaactac ggcatctagt agttcgccag tgccactacc gtttgatgat gatatgcaat
     3001 aataatcacc aagacctaat gcataaaact caactgcatc attcagttgt ttaggggaat
     3061 caactttgtt aactactaaa aatgttttct tattactatt gcgaagtaat tgtgcaatat
     3121 cctcatcaaa atcattcagg ccttcgcgcg catctgtaac aaaaaggatg atgtctgctt
     3181 cttcgattgc cagcttgact tgttttctta tttcagattc aaaaatatct tctgagcccg
     3241 atacatagcc tcctgtatca atcacagaaa aacttctccc tgtccattca cctg
//
LOCUS       DAORQT010000178         3292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5555_length_3292_cov_7.508187, whole genome shotgun sequence.
ACCESSION   DAORQT010000178 DAORQT010000000
VERSION     DAORQT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_3292_cov_7.508187"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..683
                     /locus_tag="PK134_06505"
     CDS             177..683
                     /locus_tag="PK134_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB38533.1"
                     /translation="MSESIVIDTTLSKQEKYAQLLPQAEALIKGETDAVANAANLCAM
                     LKQAFGFFWVGFYFVKQHEGKQQLILGPFQGPVACTRIELGKGVCGTAWQQKETIVVP
                     DVNNFDGHIACSSLSRSEIVVPILKHDVVAVLDVDSEHLNHFDETDKYYLEKISSLYS
                     ETFSYATE"
     gene            670..2289
                     /locus_tag="PK134_06510"
     CDS             670..2289
                     /locus_tag="PK134_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HRB38534.1"
                     /translation="MLLNKINSFNRTFLLPAVLLLFGACASVVAPTGGKKDETPPKLK
                     EVIPPEQTVNFNSEKVVFTFDEYIQLLDKDKQLVISPIIEPAPTFDVKGKKLTITFKK
                     KLKENTTYTINFGNAVADLHESNPLAGFRYVFSTGTYVDTLFVKGNVVFAENLKSEKG
                     ILVMLYTSEDDSLPYNNPPAYFTRTDESGNFSIKNIARGPYKIFALKDADNNYKFNQS
                     DEWIAFDKTPITGGDSTPINLRMFKEAEKKVYVKEAKSFNAFAFSVLMNRPVGVLNIE
                     PLQTSDNIKLISRSKTNDTLMVWLKDSISDTLSYKLSGENNWTDTLTISKPAKALRGK
                     LKQSISLLSSTNIYPGNDVLLKTAIPVASVNKEKILLMHDSTTSQPFEIVFSDSASSN
                     ITIKSVTIEKNNWNLTLLPGAVTDIYGNKNDTAKLSFHTNAFTDYGSVKIKVDGLSGS
                     NYILQLVDEKDMVISEKYIGGNGSYLFEKMLPAKCRVRIINDADGNKSFTTGNYLLKQ
                     LPELVYYYKETLTLRANWDIEINWIIKEIKQ"
     gene            2286..2705
                     /locus_tag="PK134_06515"
     CDS             2286..2705
                     /locus_tag="PK134_06515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRB38535.1"
                     /translation="MTEQQRIDSGKAPEPVGLYPHARRVGNLLFLSGVGPREKGSKKI
                     PGVEQDASGNIVQYDIAAQCHAVFKNVRYILEDSGSSWDKIIDVTVFLTNMKDDFPVY
                     NKLWAEYFKENPPCRTTVEINCLPTPIAIELKVIATI"
     gene            2708..>3292
                     /locus_tag="PK134_06520"
     CDS             2708..>3292
                     /locus_tag="PK134_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB38536.1"
                     /translation="MYLITGASGLVGSQLVFDLISMGRPVRILLRKSSSTALIKKKFK
                     SSPELFDKIKITYGDVLDIFSIEDALQDCTHVFHCAAMVSFNPQKRKEMYKVNTEGTA
                     NVVNACLEKGNIKLGFVSSVATLGRGTQQMINENDWFNQSEKNSYYAISKYAAEREVW
                     RGITEGLDAVIINPSVILGDGNWRSDSSSLFNTLY"
BASE COUNT         1121 a          516 c          650 g         1005 t
ORIGIN      
        1 atattaagtc ttatgaagac aaacatgttt tattggttga cgatgtaatc tcaacaggct
       61 caaccataat ttcatgtgca gaggcttttt caggcattag cggatgtaaa atatctatgg
      121 cttctgttgc tgctcctcat cgttgaattt ctgatatttg cacagaagtt tttagcatgt
      181 cagagtccat agttatagat acaacactaa gtaagcaaga aaagtatgca cagcttttgc
      241 cgcaggcaga agcattgatt aaaggagaga cagatgctgt tgccaatgct gccaatttat
      301 gtgccatgct caagcaggct tttggttttt tttgggtagg attttatttt gtgaaacagc
      361 atgaaggaaa acaacagttg atattaggtc cttttcaggg gcctgtggca tgtacacgta
      421 ttgaattggg caaaggagtt tgtggtacag catggcaaca gaaagaaaca attgtagtgc
      481 ctgatgtaaa taattttgat ggacacattg cctgcagctc actaagccgt tcagaaatag
      541 tagttcctat attgaagcat gatgttgttg ctgtattaga cgtggacagc gaacacctga
      601 atcattttga tgaaacagat aaatattatc ttgagaaaat aagctcactt tattcagaaa
      661 cattttccta tgctactgaa taaaataaat tcatttaata gaacttttct tttgcctgct
      721 gtacttcttt tgtttggtgc atgtgcttct gtcgtagctc ccacaggtgg taaaaaagat
      781 gaaacgccac caaaacttaa agaggtaatt cctcctgagc aaaccgtaaa ttttaatagt
      841 gaaaaagtgg tttttacttt tgatgagtac attcagttgc ttgacaaaga caagcagtta
      901 gtaatttccc caattatcga gcccgcacca acatttgacg ttaaaggtaa aaagctgaca
      961 ataaccttta agaaaaagtt gaaagaaaat actacttata caattaactt tggtaatgcc
     1021 gttgccgatt tgcatgagtc aaatccatta gcaggattca gatatgtttt ttctaccgga
     1081 acttatgttg atacactttt tgttaaagga aatgtagtgt ttgcagagaa tctgaaaagt
     1141 gaaaagggaa tccttgtaat gctttacaca tcagaagatg attcgctgcc atataataat
     1201 ccacctgcct attttaccag gacagatgaa agtggaaatt tttcaataaa aaatattgca
     1261 cgcggcccat ataaaatctt tgcattaaag gatgcagata ataattataa attcaatcag
     1321 tctgatgagt ggattgcctt tgataaaact cctataaccg gaggcgactc aacacctata
     1381 aatttgagaa tgtttaaaga agctgagaag aaagtttatg ttaaagaagc aaaatcattt
     1441 aacgcttttg catttagtgt tttgatgaac agaccggttg gagtattaaa tattgaacct
     1501 cttcaaactt ctgacaatat caaactaatt agccgcagta aaactaatga taccttgatg
     1561 gtgtggttaa aagattctat cagcgatact ttaagttata aactatcagg tgaaaataat
     1621 tggaccgata cattaacaat atcaaaaccg gcaaaagcac tcagaggcaa attaaaacaa
     1681 tcaattagtt tgctttcaag tacaaatatt tatccgggaa atgatgtgtt acttaaaaca
     1741 gctattcctg ttgcatcagt aaataaagaa aaaattttgt tgatgcacga tagcactaca
     1801 tcacaacctt ttgaaattgt tttttctgat tctgcatcat ccaacattac aattaaatct
     1861 gttactattg aaaagaataa ttggaattta actttactac cgggagctgt aacagatatt
     1921 tatggtaaca aaaatgatac tgccaaacta agttttcata ccaatgcatt tactgattac
     1981 ggaagtgtaa aaattaaagt agatggttta tcaggatcaa attatatact tcaattggtt
     2041 gacgaaaaag acatggtcat ttcagaaaaa tatattggtg gcaatggaag ctatttgttt
     2101 gaaaaaatgc ttcctgcaaa atgcagggtt cgtattataa atgatgccga tggaaataaa
     2161 tcttttacaa caggtaatta tcttttaaaa caattgcctg agcttgtata ttactataaa
     2221 gaaacactaa cattacgagc taactgggat attgaaataa attggattat taaagaaata
     2281 aaacaatgac agaacaacaa cgaattgatt cagggaaagc accggagcct gtaggacttt
     2341 atccacatgc aagacgtgta ggaaatttat tgtttttatc gggtgtgggt ccgcgagaaa
     2401 agggtagcaa aaaaattccg ggagtagagc aggatgcatc aggaaatatt gtgcagtatg
     2461 atatagcagc acaatgtcat gcagtgttta aaaatgtaag atacattctg gaagactctg
     2521 gcagcagttg ggataagatt attgatgtga ccgtttttct gaccaacatg aaagatgatt
     2581 ttccggttta caataaacta tgggcagaat attttaagga gaatccacca tgtcgtacaa
     2641 cagtggagat aaattgtctg ccaacaccaa tagctattga gttgaaagtt atagctacta
     2701 tttgattatg tatcttatta caggcgcttc aggcttggtt ggttcacaac ttgtctttga
     2761 tttaatttca atgggcagac ctgttagaat attgcttcga aaatcatctt ccacagcgtt
     2821 aatcaaaaaa aagttcaagt catcgcccga gctttttgat aaaattaaga ttacttacgg
     2881 tgatgtgttg gatattttca gtattgaaga tgcactgcag gattgcacac atgtgtttca
     2941 ctgtgcagca atggtgtcat ttaatcctca aaagcggaaa gagatgtata aagtcaacac
     3001 tgaaggaaca gccaatgtgg ttaatgcctg tttggaaaaa ggaaatataa aattgggatt
     3061 tgtaagttcc gtagcaacct taggtcgtgg cacacaacag atgatcaatg aaaacgactg
     3121 gttcaatcag agtgaaaaaa acagctatta tgctataagt aaatatgctg cagaaagaga
     3181 agtgtggcgt ggcataactg aaggattaga tgcagtcatt attaatccgt ctgttatatt
     3241 gggtgatggt aactggcggt cagattcatc atcattgttt aataccttgt ac
//
LOCUS       DAORQT010000179         3287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5571_length_3287_cov_5.847463, whole genome shotgun sequence.
ACCESSION   DAORQT010000179 DAORQT010000000
VERSION     DAORQT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_3287_cov_5.847463"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..556)
                     /locus_tag="PK134_06525"
     CDS             complement(35..556)
                     /locus_tag="PK134_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38537.1"
                     /translation="MLSSYASLNDTVKYVGKETCRSCHADIYNTFMQTGMGKSFDVAS
                     KQKTSAKFSHAVIYDKYENFYYHPYWQNDSLHLLEFRLENGDTVYKHNEVISYIIGSG
                     QHTNSHIINTNGYLNQAPATFYTQRGEWHLPPGFEDGNNTRFSRIIGLECMSCHNSYP
                     DFVLGSENNTELH"
     gene            complement(695..1213)
                     /locus_tag="PK134_06530"
     CDS             complement(695..1213)
                     /locus_tag="PK134_06530"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HRB38538.1"
                     /translation="MEELRYPIGKFQLPENITKSQFDSWKNDIAGLPKKVQQLTDSFK
                     SHHWSQTYRQEGWTALQLVHHLADSHINCFIRFKWALTEENPTIKAYDEKAWANLPDV
                     TQSSPVISIQLLHALHQRWINLINNMTEQDFEKTFFHPIQQKTHSLKGAVGLYAWHGN
                     HHLAHLKIIADK"
     gene            1362..3147
                     /gene="lepA"
                     /locus_tag="PK134_06535"
                     /pseudo
     CDS             1362..3147
                     /gene="lepA"
                     /locus_tag="PK134_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
BASE COUNT         1054 a          580 c          635 g         1018 t
ORIGIN      
        1 tacattagta tgatagactg gtgtgttgtt ttcatcagtg taattcagta ttattttctg
       61 aacccaaaac aaaatcagga taactgttat gacaactcat acattccaaa ccgattattc
      121 gtgaaaaacg tgtgttgttg ccgtcttcaa atccgggagg caaatgccac tcacctcttt
      181 gtgtataaaa agttgcaggc gcctgattca aataaccgtt ggtgttgatg atatgtgagt
      241 tggtatgctg acccgaccct ataatataac taataacttc attgtgctta taaacagtat
      301 cgccattttc aagtctgaat tctagcaagt gcaatgaatc gttctgccag tagggatggt
      361 agtaaaagtt ttcatactta tcataaatta ctgcatgact aaatttagct gatgttttct
      421 gtttggaagc aacatcaaat gatttgccca tgcccgtttg cataaacgta ttgtaaatat
      481 cggcatggca tgaacggcag gtttctttgc caacatattt tactgtatcg ttcagacttg
      541 cataggatga aagcaatggc agtgtttgct tttgttgcgt agtgcaaaaa ttcaacaata
      601 acactatcat taaaagaatg gaaaaagaaa tgagtttttg cctcataaac acaaataaaa
      661 accaaaaatc atcagcaata aaccattaac aaatttattt atcagcaata attttcaaat
      721 gtgccaggtg atgatttcca tgccatgcat aaagcccaac tgcgcctttc aggctatgtg
      781 ttttttgttg aatgggatga aaaaaagttt tttcaaaatc ttgttcggtc atattattga
      841 taagatttat ccatctctga tgcaatgcat gaagtagttg aatagaaatc accggtgatg
      901 attgagtcac atcaggaagg ttagcccatg ctttttcatc ataggcttta attgtaggat
      961 tttcttcagt caatgcccac ttaaaacgga taaaacaatt tatatggcta tcagctaagt
     1021 gatgcaccaa ctgtaaggct gtccaccctt cctgtctgta cgtttgcgac caatgatgcg
     1081 atttaaaact atctgttaac tgttgcacct tttttggcaa accggcaata tcatttttcc
     1141 agctatcaaa ctgcgactta gtaatatttt cgggcaactg aaattttcct atcggataac
     1201 gtagttcttc aataaattgt cttttattaa tcttgattta gcattattga atattctgac
     1261 aaaagtctga aaacagaagc atctttcatt tgtaaaaata tgtcttccaa actttaaaaa
     1321 accatggtat gaaggcttaa aaaagctatt tttgccgccc aatggaaaat atcagaaatt
     1381 tttgcatcat tgcccatatt gatcatggca aaagcaccct tgcagaccgt ctgcttgaat
     1441 ataccaatac tgtaagcaag cagcagatgc aggcacaggt gcttgatgat atggatctgg
     1501 aacgcgaacg cggcatcacc ataaagagcc atgccattca aatggactat actttagatg
     1561 ggaaaaagta tgtactgaat ctgatagata ctcccggcca cgttgacttt tcttatgaag
     1621 tatcaagaag tattgctgcc tgcgaaggtg cattattaat tgttgatgct gcacagggta
     1681 ttcaggcgca aaccatcagt aatctttatt tagcgcttga acacgacctt acaattatcc
     1741 ctgttttaaa caaaattgac cttccgagtg cagagcctga attggtgaaa gatcagatag
     1801 tagatttgtt aggttgcaga cgtgaagaga ttatggcagc cagtggaaaa acaggtatgg
     1861 gcgttcatga tattttgcgt gaaattgtac atcgtattcc tgcaccaaaa ggcaatccaa
     1921 aagctcctct taaagcactg atttttgatt ctgtatataa ttctttcaga ggtatcatcg
     1981 catattttaa agttattgaa ggcgaaatga aatcgggtga caaagtaaaa tttgttgcta
     2041 ccaacagtaa ttatatagct gaagaaattg gtattctgaa acttgataag atacctgcaa
     2101 agactatagg cacaggtaat gttggctata tcattacagg aataaaagat gccaaagaag
     2161 taaaagttgg tgataccatc acaactttgg aaaacccatg ttcagaagct atttccggtt
     2221 ttgaagatgt taagcctatg gtatttgccg gaatatatcc tgtagatact gaagactatg
     2281 aggagttgcg ctcatcaatg gaaaaacttc agttgaatga tgcctcattg gtttttgaac
     2341 ccgaatcgtc agcagcatta ggcttcggtt tccgttgtgg ctttttagga atgcttcaca
     2401 tggagattat tcaggaaaga ttggagcgcg agtttaacat gacagtgatt acaaccgttc
     2461 ctaacgtttc ttatgtatcg tatcttacca atggtgatgt gtatgaaatt cacaacccaa
     2521 gcgactttcc ggatcccagt aaaatggatc atattgaaga gccttatatt aaagcacaga
     2581 ttattaccaa ggcagaatat attggtgcca tcatgacttt atgtattaac aaacgtggtg
     2641 tcattatagg tcaaaactac cttacaacag accgtgtgga gctgatgttt gaaatgccct
     2701 tggcagaaat tgtttttgat ttttatgaca agttgaaatc tgtttcaaaa ggatatgctt
     2761 cgtttgatta tcatccaata ggatacaaaa ccagtgattt ggtaaagctc gatatcagac
     2821 ttaatggcga acctgttgat gcactttctg cattgattca ccgcgaccac tcatatacat
     2881 tcggcaaaaa gatttgcgaa aaattaaaag agctgattac ccgtcagcaa tttgaaatta
     2941 taattcaggc aagtataggt gctaaaatta ttgcacgcga aacagtaaaa gctatcagga
     3001 aagatgttac tgcaaaatgt tatggtggtg atatttccag aaagagaaaa cttcttgaaa
     3061 aacaaaaaga gggaaagaaa cgtatgcgtc aggtaggtag tgtagaaatt acagcaggct
     3121 ttcttagctg ttttaaaact tgactaattt ccttttctaa acattcagaa aattacttta
     3181 caaggaatca ggaagttttt ctttatcccc tttctgaatg cttttcttat ccggcatagt
     3241 gccgaaaata taatcccaca tagggtttga aacactgaaa gctgaat
//
LOCUS       DAORQT010000180         3264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5649_length_3264_cov_8.200374, whole genome shotgun sequence.
ACCESSION   DAORQT010000180 DAORQT010000000
VERSION     DAORQT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5649_length_3264_cov_8.200374"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK134_06540"
     CDS             complement(<1..707)
                     /locus_tag="PK134_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38539.1"
                     /translation="MKTESLKISLNKLIPVFMMLAGIYFGVMPYFGWDMGRIPGDLGD
                     ARFNYYILEHGYQYLTDQIHGFWDASSMYPYKNVIALSDNLIGTMPIYAIFRVFNVDR
                     ETSFQLWIIVLFLLNYWSCFYVLKKFGINNLIAASCAFIYGFGLYNHGQIFHIQVFPR
                     FIAPLVIYWSIRFIQTLKSRYFTFLLLGLAYQFYCGIYLGFFLAYFIMFLFIAAFIVN
                     LKLPFELNKKALLNYTLS"
     gene            complement(738..1454)
                     /locus_tag="PK134_06545"
     CDS             complement(738..1454)
                     /locus_tag="PK134_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB38540.1"
                     /translation="MIQKLSIIIPAYNEARTIHLILDKVLAVKLTNNISKEIILVNDC
                     SKDTTKEVIEKYITDHPEADMRLFNQPVNMGKGAALHKGIELATGEYLIIQDADLEYD
                     PEEYNILLKPILTGFADVVYGSRFLGGKPHRILFFWHSIGNKFLTFCSNMFTNLNLTD
                     METCYKMFNTKMVQSLKLKEKRFGFEPEVTAKIARIPNVRIYEVGISYYGRTYAEGKK
                     IGWKDGFRALWCIVRYGLSH"
     gene            complement(1471..1764)
                     /locus_tag="PK134_06550"
     CDS             complement(1471..1764)
                     /locus_tag="PK134_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38541.1"
                     /translation="MDWLVVVAALLLIILLFVFPLAKIIYKVWSLVTTSIGRLISKIA
                     LFIIYFGIFWPTALIRKMFGKGNQFYRQKKSDELTYFSNRNKKFNTDDFKNPW"
     gene            complement(1866..2015)
                     /locus_tag="PK134_06555"
     CDS             complement(1866..2015)
                     /locus_tag="PK134_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRB38542.1"
                     /translation="MELLSDLFRFIIQRKKYWLAPLIIVILLMSVLIIAGSNPAFAPF
                     IYTLF"
     gene            2128..>3264
                     /locus_tag="PK134_06560"
     CDS             2128..>3264
                     /locus_tag="PK134_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRB38543.1"
                     /translation="MLLKQEIEKRRTFAIISHPDAGKTTLTEKLLLFGGAIQVAGAVK
                     SNKIKKTAASDFMEIEKQRGISVATSVMTFFYKNCQINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVKGVEPQTEKLMEVCRMRNTPVIIFINKMDREGQNPFDLLDELEEKLF
                     IHTRPLSWPISSGSTFQGVYNLHNKSLMLFRANQKASDSDVLPIKDMQDPLLDKQLGR
                     YVDQLREDVELIEGVYEPFNKQLYSEGLLAPVFFGSGINNFGVKELLDTFIEIAPEPK
                     SRQTDQRTVTPEESKFSGFVFKIHANIDPRHRDRIAFLRVCSGMFERGKFYHHVRLNK
                     DLRFNNPATFMAQEKSIVEQAFPGDVIGLYDTGNFKIGYTLTEGE"
BASE COUNT         1141 a          546 c          561 g         1016 t
ORIGIN      
        1 gataaagtat agttcaataa agccttttta tttaattcga atggtagttt taaattaaca
       61 ataaatgctg caataaaaag aaacataata aagtaagcta agaaaaaacc taaatagatt
      121 ccacaataaa actgatatgc taaacccaat aataaaaaag taaaatatct acttttcaag
      181 gtttgaataa acctgattga ccaatagata actaagggtg caataaatct aggaaaaacc
      241 tgaatatgaa aaatttgccc atggttgtac aatccaaatc cataaataaa tgcacagctt
      301 gctgcaatga gattattaat gccgaatttt tttagaacgt aaaaacatga ccaatagttc
      361 aaaaggaaaa gcacaatgat ccaaagttga aaagatgttt cacgatcaac attaaaaact
      421 cggaagatgg catatatagg cattgtacca attaaattat ccgatagtgc aattacattc
      481 ttataaggat acattgaaga ggcatcccaa aacccatgaa tctgatcagt taaatattga
      541 tatccatgct caagaatata atagttaaaa cgggcatcac ctaaatcacc gggaatgcgc
      601 cccatatccc atccaaaata gggcatcact ccaaaataaa tccctgctaa catcataaaa
      661 acagggatta atttatttaa tgaaattttc aggctctcgg tcttcatctg tttattagct
      721 aaaattaaaa taaaaaatta atggcttaat ccatatctaa caatacacca caatgctctg
      781 aagccatctt tccatccaat cttttttcct tcggcatagg tccgtccata gtacgaaatt
      841 ccaacttcat aaatacgtac attaggtatt cttgcaattt tagcagttac ttccggttca
      901 aaaccaaatc ttttttcttt gagctttagt gattgtacca tttttgtatt aaacatttta
      961 tagcaagttt ccatgtctgt taggttcaaa ttggtgaaca tattgctaca aaatgtcaag
     1021 aatttatttc cgatggaatg ccagaagaat aaaattctat gtggcttacc acctaaaaaa
     1081 cgtgatccat aaactacatc tgcaaaacct gtaagaattg gttttaacaa aatattgtac
     1141 tcttccggat catattcaag atcggcatcc tgaatgatta aatattctcc tgttgccaat
     1201 tcaatgccct tatgcagtgc tgcgccttta cccatattaa caggctgatt aaaaagtctc
     1261 atgtcagcct ctgggtgatc tgtaatatat ttctcaatta cctctttggt agtatctttt
     1321 gaacagtcgt ttacaagaat aatttctttg ctgatgttat tagtcagttt aactgctaaa
     1381 actttgtcaa gaattaaatg aatggttcgc gcttcgttat atgcaggaat aatgatggaa
     1441 agtttctgta tcattttgct ttaatgtcaa ttaccaggga tttttgaaat catcggtatt
     1501 aaacttttta tttcggttgc taaaataggt taattcatct gatttttttt gccgatagaa
     1561 ttgattgccc tttccaaaca ttttgcgtat caatgctgtt ggccaaaata taccgaagta
     1621 aataataaaa agtgcaattt ttgatattaa tcttccaata gaagtagtta ctaaagacca
     1681 aaccttataa ataatttttg caagcggaaa aacaaataat aaaataatca gtaataaagc
     1741 cgcaactact acaagccaat ccaagctgtt aaaattttct gctttatact tctgataaag
     1801 aaaaaccatt aataaaatga ggcatattga agaaaaagtt tgccaaattt gcttattcaa
     1861 cataattaaa acagggtgta aataaacggt gcaaatgcag gattgcttcc ggcaattatc
     1921 aatacactca ttaatagaat gacaataatt aatggtgcca gccagtattt ttttcgctga
     1981 attataaatc tgaaaagatc tgatagtaat tccattgact tgagtgttac aaatatacaa
     2041 tatttaaatt tcttttgtta tatacagtgt aaggcactat tgtggcaaat gaaatgctat
     2101 ttttgcagac tgaaaaaaat atccggaatg ttgcttaaac aggaaattga gaaacgaaga
     2161 acttttgcaa taatatcgca ccccgatgca ggtaaaacaa ctttaacaga gaagttgctg
     2221 ttgtttggtg gtgctataca ggttgcaggt gcagttaaat cgaacaaaat aaagaagact
     2281 gctgccagcg attttatgga aattgaaaaa caaagaggta tttctgttgc aacatcagtg
     2341 atgactttct tttataaaaa ctgccagata aatttattag acactccggg ccacaaggac
     2401 tttgctgagg atacatacag aacattaacc gctgtggata gtgtaattct tgtaattgac
     2461 tctgtgaaag gggtagagcc acaaacagaa aagttgatgg aggtatgccg catgcgaaat
     2521 actccagtaa ttattttcat caataagatg gatcgtgaag gtcagaatcc atttgattta
     2581 ttagatgagt tggaagaaaa actttttatc catacacgtc cactaagttg gcccatcagt
     2641 agcggatcta cttttcaggg cgtttataac ttacacaata aaagtctgat gctttttaga
     2701 gcaaaccaaa aggcttctga cagtgatgtt cttccgatta aagatatgca agacccacta
     2761 cttgacaaac agcttgggcg ctatgttgat cagcttcgtg aagatgttga gttgatagaa
     2821 ggtgtttatg agccattcaa caaacaactc tatagtgaag gcttacttgc accggtattt
     2881 tttggtagtg gtattaataa ttttggtgtt aaagaactgt tagatacttt tattgaaatt
     2941 gcgcctgagc ctaaatcccg acagacagac caacgtacag tgacaccaga agaaagtaag
     3001 ttctccgggt ttgtgtttaa gatacatgcc aatattgacc ctcgtcatcg tgacagaata
     3061 gcttttttaa gagtatgttc cggcatgttt gaacgtggta agttttatca tcatgtcagg
     3121 ttgaataaag atttacgatt caacaatccg gcaactttta tggctcaaga aaaaagtatt
     3181 gtggagcaag cttttccggg tgatgttata ggactttatg ataccggaaa ctttaaaatt
     3241 ggttatacac ttacagaggg ggag
//
LOCUS       DAORQT010000181         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5660_length_3260_cov_4.998128, whole genome shotgun sequence.
ACCESSION   DAORQT010000181 DAORQT010000000
VERSION     DAORQT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_3260_cov_4.998128"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PK134_06565"
     CDS             <1..707
                     /locus_tag="PK134_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRB38544.1"
                     /translation="MRIGGGQEIGMACDFTIASDMARFGQAGPKHGSAPIGGATDFLP
                     LFVGIERAMMSLTLCEPWTAHQAYYFGMLTDVVPVLKVNGKFIANPLVTIDRTTDEYG
                     RFVFGTMKKDDDLKKAKELVAAGETDFTMLDAAVNGLIAKILTTFPNCMNKTISEVRK
                     FKLEHWDKNKESSREWLALNMMSEAKAGFKAFNDGPKENREVDFIKLRQLLAEGHEWN
                     EDLHRTISPQYQEVKQ"
     gene            710..1483
                     /locus_tag="PK134_06570"
     CDS             710..1483
                     /locus_tag="PK134_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRB38545.1"
                     /translation="MEKIKVSYLYDDTIANIVLDDGKGNVMDHIMMEDLQNVFDSFSE
                     KKNIKLIVIEGAGKHFSFGASVEEHQKEFAGAMLNAFHKLFYTLRDLSIPVAAKVSGQ
                     CLGGGMELALMCNFIFADKTAKFGQPEIILGVFPPPASILLAEKIGLARAEDLLITGK
                     TITADEAKSIGLLNDVFDDKETLNAGLNNWIETNILPKSASSLRFAVKAVRAKMNHIL
                     SNFLPQLESMYVNQLMQTEDANEGINSFLEKRKPVWKNS"
     gene            1493..>3260
                     /locus_tag="PK134_06575"
     CDS             1493..>3260
                     /locus_tag="PK134_06575"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HRB38546.1"
                     /translation="MKNIRTVGVVGAGTMGAALAQKFAQEGFEVILADRAKNFVEKGL
                     NNIRITLEDGVTKNVFSKQQVDTFLSHLKGTDSLDDLKVCDLVIEAIFEDLQAKKDLF
                     SSLSSIVSSDGIVATNTSSFSVTELAASMVNPERFIGLHFFYHAAKNRLVEIIPGEKT
                     TSETLQAARLFSVLAGKDAIDCTDTYGFAVNRFFVPWLNESVKLLQEGIATKSEIDAV
                     CMKVFGIGMGPFALMNATGVPIAYHAEKTLEVFGKLYEVASLLKSQAEAAKPWEIEPA
                     TADTIESKKVKLISDRMLGVVFYVCSQILEEKVSSAIHLNRGAKIGLRWKKGPVDLMK
                     SAGETEVIRLVTLMSELYQMRIPSSVGKKFWQMESVTIKKNKSVAVITMDEPENMNAL
                     SEETMQGLSEKYAEADNDPTIETIFITGSGKAFVAGADIKFFVRNIKTGKISDIETFT
                     AFGQDVFNRIDRSNKKVVAIVNGMALGGGLELALCADRILAFPKAQFAFPETGIGIYP
                     GLGGTQRSAAKIGQGLSKYLIHTGKMLNAKDAEEIGLVDKIITVEDFMEMVEGKKQVP
                     ASSEKALPGKWSMIKDFFEDNTL"
BASE COUNT         1047 a          559 c          758 g          896 t
ORIGIN      
        1 gtatgcgtat tggtggtgga caggaaatag gaatggcttg tgattttacc attgcaagtg
       61 atatggcacg ttttggccag gctgggccta aacacggtag cgcaccaatc ggtggcgcta
      121 cagatttttt accactgttt gttggtattg aacgggccat gatgtcgctt acgttatgcg
      181 aaccatggac agcacatcag gcttattatt ttggaatgct tactgatgtg gttccggttt
      241 taaaagtaaa cggaaaattt attgccaatc cattggtaac tattgacaga acaactgatg
      301 aatacggacg ctttgttttc ggcaccatga aaaaggacga tgacctgaaa aaagcaaaag
      361 aattggttgc tgcaggcgaa acagatttca ccatgctgga tgcagcagtc aacggattga
      421 ttgcaaaaat ccttaccacc tttcctaatt gcatgaataa aaccatcagc gaagtgcgga
      481 aattcaaact ggaacattgg gataaaaaca aagagagcag ccgtgagtgg cttgctttaa
      541 atatgatgag cgaagcgaaa gccggtttca aagcatttaa cgatggaccg aaagaaaaca
      601 gggaagttga ttttataaaa ctacgtcaac tgctggcaga agggcatgaa tggaatgaag
      661 atttgcacag aactatttcg ccacaatatc aggaggtaaa acaataagca tggaaaaaat
      721 caaggttagt tatttgtatg atgatacgat cgcaaacatt gtgcttgacg atggcaaagg
      781 caatgtaatg gatcatatca tgatggaaga tttgcaaaat gtgttcgatt cattttcgga
      841 aaagaaaaat attaaactga tagtgattga aggcgcagga aaacattttt catttggcgc
      901 aagtgttgaa gaacatcaaa aagaatttgc cggtgccatg ctaaatgctt ttcataaatt
      961 attttataca ctcagggatt tgtcaattcc cgttgcagca aaagtatccg ggcaatgttt
     1021 gggtggagga atggaacttg cgttgatgtg caattttatt tttgctgata aaactgcaaa
     1081 gttcggtcag cccgaaatta tacttggtgt tttcccgcca ccggcttcta ttctgctcgc
     1141 tgaaaaaatt ggcttggctc gggccgaaga cttattgatt acaggcaaaa ccattactgc
     1201 cgatgaagca aaatcaatcg gtttactgaa tgatgtattt gatgataaag aaacattgaa
     1261 tgcagggtta aacaactgga ttgaaacaaa cattttaccc aaaagcgcat cttctttacg
     1321 ttttgcggtg aaggcggtaa gagcaaagat gaatcatata ctgagcaatt ttcttccgca
     1381 gttggaatcc atgtatgtaa accaactgat gcaaacggaa gatgccaatg agggcattaa
     1441 ctcattttta gaaaaaagaa aaccggtttg gaagaattca taataaaaag caatgaaaaa
     1501 tatcagaaca gtaggagtag ttggcgcagg aacaatgggc gcagcattgg ctcaaaaatt
     1561 tgcacaggaa ggctttgagg ttattcttgc cgaccgtgca aaaaatttcg ttgagaaagg
     1621 attgaacaat atccgcatca cattggaaga tggagtaaca aaaaatgttt tttcaaaaca
     1681 acaggtagat acatttctat cacatttaaa gggaaccgat tcactcgatg acctgaaggt
     1741 ttgcgacctg gtgatagaag caatttttga agacctgcag gcaaagaaag atttgttcag
     1801 ctcgctgagt agtattgttt caagcgatgg tattgttgcc acaaacactt cttcattttc
     1861 tgtaaccgaa ctggcagcaa gtatggttaa cccagaacga tttatcggac ttcacttttt
     1921 ttatcatgct gccaaaaatc gtctggtaga aattattccc ggtgaaaaaa ctacttccga
     1981 aactttacag gcagcccggt tattttctgt gctggcaggc aaagatgcta ttgattgcac
     2041 cgatacatac ggatttgctg tgaaccgttt tttcgttcca tggcttaatg agtcggttaa
     2101 actgttacag gaaggcatag caacaaaatc agaaattgat gcagtatgca tgaaagtatt
     2161 tggtattggt atgggtccct ttgcattgat gaatgcaaca ggagttccta ttgcttatca
     2221 cgctgaaaaa actttggagg tttttggtaa actttatgaa gtagcctccc tgttaaagtc
     2281 gcaggcagaa gctgccaaac cttgggaaat agaacctgca acggctgata caattgaatc
     2341 aaaaaaagta aaattaatca gcgaccgtat gttgggtgtt gtgttctatg tgtgttcgca
     2401 gattcttgaa gaaaaagttt cttcagctat tcaccttaac cgtggtgcaa aaattggctt
     2461 gcgttggaaa aaaggaccgg ttgatttaat gaagtcggct ggagaaacag aagtaatccg
     2521 ccttgtaaca ttgatgtctg aattatacca gatgcggatt ccatcatctg tcggaaaaaa
     2581 attctggcaa atggaatcgg ttacgattaa gaagaacaag tcagtggctg tgattaccat
     2641 ggatgagccg gaaaacatga atgcgctgag cgaagaaacc atgcagggcc tttcagaaaa
     2701 atatgctgaa gctgataatg acccaaccat tgaaacaatt ttcatcaccg gttcagggaa
     2761 agcatttgtt gccggtgctg atattaaatt ttttgttaga aatatcaaga caggaaaaat
     2821 cagcgacatt gaaacattca ctgcctttgg tcaggatgta ttcaaccgta ttgaccgttc
     2881 aaataaaaaa gtagtggcaa ttgttaacgg catggcgctt ggtggcggtc ttgaactggc
     2941 attgtgtgcc gacaggattc ttgcatttcc aaaagcacag tttgcatttc ctgaaactgg
     3001 cattggcatt tatccgggat tgggtggtac gcaacgcagt gctgctaaaa ttgggcaagg
     3061 actgagtaaa tatttaattc atacgggcaa aatgctcaat gcaaaagatg cggaagaaat
     3121 tggtttggtt gataaaatta ttacagttga ggatttcatg gaaatggttg aaggcaaaaa
     3181 gcaggttcct gcttcaagcg aaaaggcatt accgggaaaa tggagcatga taaaagactt
     3241 tttcgaagac aatacacttt
//
LOCUS       DAORQT010000182         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5677_length_3252_cov_5.090084, whole genome shotgun sequence.
ACCESSION   DAORQT010000182 DAORQT010000000
VERSION     DAORQT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_3252_cov_5.090084"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK134_06580"
     CDS             <1..262
                     /locus_tag="PK134_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38547.1"
                     /translation="VPAHTCWKCNYRNATLSENEHLFQMGIVLGVGEHDFCIAQNKRL
                     SEIMTKKGMAHWLDIRPGANHDWPVWREMFPAYIEALQTAKV"
     gene            543..779
                     /locus_tag="PK134_06585"
     CDS             543..779
                     /locus_tag="PK134_06585"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRB38548.1"
                     /translation="MENQKDKSEIEDVREDVWRRFEKDNQRGKLFGGLIIICVGALLF
                     AREAGFYFPSWIFSWPMILIVIGIIHSEIWHGWY"
     gene            770..1330
                     /locus_tag="PK134_06590"
     CDS             770..1330
                     /locus_tag="PK134_06590"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HRB38549.1"
                     /translation="MVLVLIGGLFLIRNSFPIFEYRNFIWPVVIILLGLFIILKPSNR
                     FRRACRRGRFHPRHPQRQFGNYTGTPSSGEDFLEAVAVFGSIKKNIISKDFKGGEVSA
                     VFGGAEINFMQADIQGRVELEVNQVFGGTKLIVPAHWEIKSEIAAVLGSVEDKRPLRS
                     ASLTDEKKVLILRGTTVFGGIDINSY"
     gene            1366..1704
                     /locus_tag="PK134_06595"
     CDS             1366..1704
                     /locus_tag="PK134_06595"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HRB38550.1"
                     /translation="MYFIAKVIYQIVINGSKNNQFDEQLLIIKAQTVADAYLKARAIG
                     AEKQHEFINDRKNPVMWKMIDVADIIPVESPEHGSVVYSQVIEEDSESFIDYIKRKAL
                     TLQTQSLLFA"
     gene            1711..2736
                     /locus_tag="PK134_06600"
     CDS             1711..2736
                     /locus_tag="PK134_06600"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRB38551.1"
                     /translation="MIIEKIKNSIDQIVWWLCWYFFVAALVHQFGFDWCLSFADSTVM
                     LLLLLLTSCSTYFAFNKFQKTGNRFFVILWNIALSWISVFILGLVLRYIYVDDLKYLT
                     FLDSSMVVRFTWFFIMNLLVTVIYGLQVNILEQKKLEAHNNENANLMKDAELSGLRLQ
                     LQPHFLFNSLNSINALIGTEPAKARSMVLQLSEFLRGTLQRDNKKLISLQEEISHLKI
                     YLEIEKVRFGHRLITQIEIPENCLNMMVPPLILQPIVENAIKFGLYDTTGQVTISLKV
                     SCMESHLRIFVANPFDSDTAASQKGTGFGLDAVQRRLYLLYARNDLLKTEKENSIFIT
                     QIDIPQI"
     gene            2733..>3252
                     /locus_tag="PK134_06605"
     CDS             2733..>3252
                     /locus_tag="PK134_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38552.1"
                     /translation="MIKAIVIDDEPLARSLVLEFLKAFPNIEVVQECNDGFEGLKAIQ
                     QFQPQLIFLDIQMPKINGFEMLELIENPPGVIFTTAFDNYAIKAFEAHAIDYLLKPFS
                     EERFNKAVAKFLATNNKAPAMDLVSAESNILPDEKNRIVVKDGSKITIIPVDDVIYIE
                     AYDDYVKVHTQNG"
BASE COUNT         1023 a          491 c          677 g         1061 t
ORIGIN      
        1 agtgcctgcc catacttgtt ggaaatgcaa ttaccggaat gccactctat ccgaaaatga
       61 gcatttgttt cagatgggaa tagttcttgg tgtaggcgaa catgattttt gtatagcgca
      121 gaacaaacgc ctttcagaaa ttatgactaa aaaaggtatg gctcattggc tcgatattcg
      181 gcccggtgcc aaccacgact ggcctgtgtg gcgcgaaatg tttccggcat acattgaagc
      241 attgcagaca gctaaggttt agattttttt gagagttgat caaacattgt tgacaggagt
      301 gatcaagtga ggagtgatga ggtgacgaag tcaatttgat aatcagatac ggctgacaag
      361 tttgataatt ctgaattcag aactctacat tttgcattga tttcctttac cgacactagc
      421 ctgccgttta ccgatatatt tttctattca ctgagtttgt agctgctata gacctattgt
      481 gtctgtgaac tacctttgaa gtcaacatat ctttgtccaa taatatttaa agaaataata
      541 aaatggaaaa tcaaaaagat aaatctgaaa ttgaagacgt gagagaagac gtatggcgca
      601 gatttgagaa agataatcaa cgcggtaaat tgtttggtgg cttaataata atttgtgttg
      661 gtgcattgtt gtttgccaga gaagccggtt tttatttccc gtcatggatt ttttcatggc
      721 ctatgatttt gatagttata gggataattc attccgaaat atggcatgga tggtattagt
      781 acttattggc ggattgtttt tgatacgaaa cagtttcccg atttttgaat atagaaattt
      841 tatctggcct gttgttataa ttctactggg gttgtttatt attcttaaac cttctaatcg
      901 ttttcgcaga gcttgcaggc gaggcaggtt tcacccaaga catccgcaaa ggcaatttgg
      961 aaactatacc ggtacgccat cttcaggtga agattttttg gaagcagtcg cagttttcgg
     1021 aagtataaaa aaaaacatca taagtaagga ctttaaaggt ggtgaagtgt cggctgtgtt
     1081 tggtggtgca gaaattaatt ttatgcaagc cgatatacag ggtagagtag agctggaggt
     1141 gaatcaggtt tttggaggaa caaaacttat tgttccagca cattgggaga ttaagtcaga
     1201 aattgctgca gtgttaggaa gtgttgaaga caaacgtcca ttgcgtagtg catcgctaac
     1261 agatgaaaag aaagtactga tattacgagg tactactgta tttggaggaa tagacattaa
     1321 tagttattaa attttaaatt cattttttaa tcaaactaaa ttgaaatgta ttttatagct
     1381 aaagtaattt atcagatagt aatcaacgga agtaaaaata atcagtttga tgagcaactt
     1441 ttaatcatta aagcacaaac tgttgccgat gcttatttaa aagcacgtgc aattggcgca
     1501 gagaagcaac atgagtttat taatgataga aaaaaccctg ttatgtggaa aatgattgat
     1561 gttgcagata ttatacctgt tgaatcaccc gagcatggtt ctgttgtata ttcgcaggtt
     1621 attgaagaag actctgagtc gtttattgat tatattaaac ggaaagcact gacacttcaa
     1681 acgcaatcac ttttgtttgc ctgataaata atgattattg aaaaaataaa aaattcaatt
     1741 gaccagattg tttggtggct atgctggtat ttttttgttg cagcattggt tcaccaattt
     1801 ggatttgact ggtgcctgtc ttttgcagac agcactgtaa tgttactatt attgttgctt
     1861 acttcgtgca gcacctattt tgcatttaat aagtttcaaa aaacaggaaa tcgttttttt
     1921 gtgatattat ggaatattgc tttatcgtgg atttctgttt ttatattagg cttggtgcta
     1981 aggtatattt atgttgatga tttaaaatat ttgacttttc tcgattcatc gatggtggtt
     2041 cggtttacat ggttttttat aatgaatctt ttagtaactg tcatctatgg tttgcaagta
     2101 aatattttgg agcagaaaaa attagaagca cataataatg aaaatgccaa cctgatgaaa
     2161 gatgcagaat tatccggatt acgattacaa cttcaaccac attttttatt caacagcctg
     2221 aattctatta atgcattaat aggaacagaa cctgcaaagg caagaagtat ggtgttacag
     2281 ctttcagaat ttttacgcgg cactttacaa agagataata agaaattgat aagccttcag
     2341 gaggaaatca gccatctgaa gatttatctt gaaattgaaa aagtgcggtt tggtcaccga
     2401 ttgattacgc aaattgaaat tcctgaaaac tgtttgaata tgatggtgcc accgcttatt
     2461 ttacaaccta ttgtagagaa tgctattaaa ttcggactct atgacaccac aggtcaggtc
     2521 acaatttcat taaaggtttc ctgcatggaa agtcatttaa ggatattcgt tgccaaccct
     2581 tttgattctg acactgctgc atcgcagaag ggcaccggct ttggccttga cgcagtgcaa
     2641 aggcgattgt atctgcttta tgcacgtaac gatttactta aaacagaaaa agaaaatagt
     2701 attttcatta cacaaataga cattcctcag atatgattaa agcaattgtg atagatgatg
     2761 agcctttagc aagaagtctt gtgttggagt ttcttaaagc gtttccaaac attgaagtgg
     2821 tacaggaatg taatgatggc tttgaagggt tgaaagccat tcagcagttt caacctcaac
     2881 ttatttttct ggatatacaa atgccaaaga ttaatggttt tgagatgctt gagttgattg
     2941 aaaacccgcc cggtgtaatt tttacaactg catttgacaa ttatgcaatt aaagcttttg
     3001 aagcgcatgc catagactac cttttgaaac cattcagcga agaacgattt aacaaggctg
     3061 ttgctaaatt tcttgcaacc aataataaag cacctgcaat ggatttagta agtgcggaaa
     3121 gcaatattct gcctgatgaa aagaatagaa ttgttgtaaa agatggaagt aaaattacga
     3181 tcattcctgt tgatgatgta atttatattg aagcttatga tgattatgtg aaagttcata
     3241 cacaaaatgg tt
//
LOCUS       DAORQT010000183         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5714_length_3240_cov_5.923705, whole genome shotgun sequence.
ACCESSION   DAORQT010000183 DAORQT010000000
VERSION     DAORQT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5714_length_3240_cov_5.923705"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK134_06610"
     CDS             <1..382
                     /locus_tag="PK134_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB38553.1"
                     /translation="FKFVNGGMGSIAYTTSVWDVNLESSITVMGKYGSIKIGGQYMNE
                     VEYCHIKDYTMPKLDPVNPANDYGAYKGSAANHHYIIENVADVLRGRTSITTNALEGL
                     KVVEIIERIYALRKIEKSEIKFHL"
     gene            388..>531
                     /locus_tag="PK134_06615"
     CDS             388..>531
                     /locus_tag="PK134_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRB38554.1"
                     /translation="MYQEILDKKAKVAVIGLGYVGLPIALEFAKKISVIGFDINAERV
                     EKMQ"
     assembly_gap    532..603
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <604..1692
                     /locus_tag="PK134_06620"
     CDS             <604..1692
                     /locus_tag="PK134_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRB38555.1"
                     /translation="KFTNSLDVLREARFFIVAVPTPIDDHKLPDLKPLVGASTSIGKV
                     LKKGDYVVYESTVYPGCTEEDCIPVLEEHSGLKFPNDFKVGYSPERINPGDKEHTITK
                     IKKVVSGCDAESLDVIAKVYETIVTAGVHRASSIKVAEAAKIIENTQRDVNIALMNEL
                     SIIFNKMGINTYEVIEASSTKWNFLRFFPGLVGGHCIGVDPYYLTYKANELGYHSQVI
                     NSGRYVNDSMGFYVAKQTVKKMIAKGKDISKSKVLVMGATFKEDVEDIRNSKVTDVVN
                     EFKSFGVTIEVTDPYASSDELMHEYGFGLVDKIANDYDAVVVAVSHKDYLKMDENYFK
                     SICKPDGVIVDIKGFLRNKIKNLQYWSL"
     gene            1759..2784
                     /gene="galE"
                     /locus_tag="PK134_06625"
     CDS             1759..2784
                     /gene="galE"
                     /locus_tag="PK134_06625"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRB38556.1"
                     /translation="MKVLVTGGTGYIGAHTVVELMNSGFDVEIIDNLNNSKEEVVDAI
                     AKITGKRPIWFNIDLCDASELKNYFSKYKPDAVVHFAAHKAVGLSVEQPLEFYRNNLL
                     SLINILQCCVDAKCNNFVFSSSCSVYADPDKLPIDENAPVKTAKSPYGNTKKIGEEII
                     TDTVKSSLLQAVSLRYFNPVGSHDSALIGELPTTMPLNVMPIITQVAIGKRKQFVVFG
                     NDYPTADGSCVRDYIHVVDVAKAHVTAVKRLLNKKNNSAYEVFNLGTGKGFSVLELIH
                     AFEKTTKQKLNYTIEGRRAGDVTAVYADTTLANTVLGWHTERDLNNMIRTAWNWELYL
                     AGRTDFI"
     gene            2829..>3240
                     /locus_tag="PK134_06630"
     CDS             2829..>3240
                     /locus_tag="PK134_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB38557.1"
                     /translation="MPKSTIIITGGAGFIGSHVVRRFVNNYSDYTIYNLDNLTYAGNL
                     ANLKDIESKSNYHFIKGDITDADFVNKLFADINPDAVIHLAAESHVDRSITNPLDFIM
                     TNIVGTVNLLNAARNTWIKDKKVSPGKRFYHISTD"
BASE COUNT         1040 a          557 c          635 g          936 t
ORIGIN      
        1 ctttaagttt gtaaacggtg gaatgggtag tattgcctac actacctctg tttgggatgt
       61 aaaccttgaa agcagtatta ccgtaatggg gaaatacggc tctataaaaa ttggcggtca
      121 gtatatgaat gaagtagagt actgtcatat caaagactat acaatgccta aacttgaccc
      181 ggttaatcct gcaaacgatt acggtgccta caaaggctct gctgccaacc atcactacat
      241 aattgaaaat gtggcagatg ttttgcgtgg tcgcacttca attactacca atgcccttga
      301 gggattaaaa gtagtagaaa tcattgagcg tatttatgcg cttcgtaaaa tagaaaaatc
      361 agaaataaaa tttcatcttt aaaataaatg tatcaggaga tattagataa aaaagcaaaa
      421 gttgctgtta ttggcttagg ctatgttgga ttacctattg cactggaatt tgcaaaaaag
      481 atttccgtaa taggattcga catcaatgca gagcgagttg aaaaaatgca annnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnaaattca ccaactcatt ggacgtactt agagaagcgc ggttttttat tgtagctgtt
      661 cctactccta ttgacgatca taaactacct gacctgaaac ctttagttgg tgcatcaact
      721 tctattggca aggtactgaa aaaaggtgat tatgttgtgt atgaaagtac cgtttatccg
      781 ggttgtacag aagaagattg tattcccgta cttgaagaac attccgggtt aaaatttcca
      841 aatgatttta aagtaggcta ctcacctgaa cgtattaacc ccggtgataa agaacatacc
      901 atcacaaaaa tcaagaaagt agtttcagga tgcgatgcag aatcgcttga tgttattgca
      961 aaggtttatg aaacaattgt aacagcaggt gtgcatcgtg ccagcagcat taaagttgcc
     1021 gaagctgcaa aaattattga aaacacacag cgtgatgtaa acattgcact gatgaatgag
     1081 ctttccatca tcttcaataa aatgggaata aatacctatg aggtgattga ggcaagcagt
     1141 acaaaatgga acttccttcg cttttttccg ggcctggtag gcggacactg tattggtgtt
     1201 gacccttatt atcttaccta taaagccaat gaactgggat atcattcaca ggtcatcaac
     1261 tccggtcgtt atgtaaatga cagcatgggc ttttatgttg ccaagcaaac cgtaaagaaa
     1321 atgattgcca aaggcaagga tatttctaaa tcgaaagtgc tggtgatggg tgctacattt
     1381 aaagaagatg tagaagatat ccgcaacagc aaggttacgg atgtagtgaa cgaatttaaa
     1441 tcatttggtg taacaataga agttaccgac ccttatgctt cttctgatga gttgatgcat
     1501 gaatatggtt ttggtttggt tgataaaatt gccaatgatt atgatgctgt agtggttgct
     1561 gtttcgcata aggattatct taagatggat gaaaattatt ttaagagtat ctgtaaaccc
     1621 gatggtgtta ttgttgacat aaaaggattt ttaagaaata aaatcaaaaa tcttcaatac
     1681 tggagtctgt aaacctttag tgcggcaacg ccctaaccgt tatacagaaa tattaacccg
     1741 gtaaatttat tgcatgtaat gaaagttttg gtcaccggag gcaccggtta tattggtgca
     1801 cacactgttg tagaactcat gaacagtggt tttgatgtag aaatcattga caacctgaat
     1861 aattcaaaag aagaagttgt tgatgctatt gcaaaaatta ccggcaaacg cccgatatgg
     1921 tttaacattg acctttgtga tgccagcgaa ttaaaaaact atttcagcaa gtataaaccg
     1981 gatgcagttg tacactttgc agcacataaa gcagtagggc tttctgttga acaaccattg
     2041 gaattttatc gaaacaacct gctttcgctc attaatattt tgcaatgttg tgttgatgca
     2101 aagtgcaata attttgtttt ttcatcaagt tgctctgttt atgctgatcc tgataaatta
     2161 cctattgatg aaaatgctcc tgtcaaaaca gctaaatcgc cttatggcaa caccaaaaaa
     2221 attggagaag aaataataac agacactgta aaatcatctt tactgcaagc tgtttcactt
     2281 cgctatttca atcccgttgg ctcacacgat agtgctttaa tcggtgaact gcctacaacc
     2341 atgccattga atgtaatgcc catcatcact caggtagcca ttggtaaacg caaacagttt
     2401 gtggttttcg gaaacgatta tccaacagcc gatggcagtt gtgtaagaga ttatatacat
     2461 gtagttgatg tggcaaaggc acatgttaca gctgtgaaac gattgctcaa caaaaaaaac
     2521 aactctgctt acgaagtatt taatctggga acaggaaaag gattttctgt acttgaactt
     2581 attcatgcat ttgaaaaaac cacaaaacag aaattaaatt ataccattga aggaagacgt
     2641 gccggtgatg tcactgctgt ttatgccgac acaacattgg ctaacaccgt acttggatgg
     2701 catacagagc gcgatttgaa taatatgata cgtactgcct ggaactggga gttgtatctg
     2761 gccggaagaa cagactttat ctgagctaaa ggttctttat taagttttaa ttttttaatt
     2821 ttaccctcat gccaaaaagt acaatcatta tcacaggcgg tgccggattc ataggttcgc
     2881 atgttgtgcg cagatttgta aacaattatt ccgattacac aatttacaac ctcgacaact
     2941 taacctatgc aggaaatctt gctaacctga aagatataga atcaaaatca aactatcatt
     3001 ttataaaagg tgatattacc gatgctgact ttgtaaacaa actgtttgca gatataaatc
     3061 ccgatgcagt aattcatctt gctgcagaat cgcatgtgga cagaagtatt accaacccac
     3121 tcgattttat catgaccaat attgtgggta cggttaacct tcttaatgct gcacgaaata
     3181 cgtggataaa agacaaaaaa gtttctcccg gaaaaagatt ttatcatatc agtactgatg
//
LOCUS       DAORQT010000184         3233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5737_length_3233_cov_6.809629, whole genome shotgun sequence.
ACCESSION   DAORQT010000184 DAORQT010000000
VERSION     DAORQT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_3233_cov_6.809629"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /locus_tag="PK134_06635"
     CDS             <1..2084
                     /locus_tag="PK134_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRB38558.1"
                     /translation="RNLAEKMEGFPESIKFLKKNSDSFKDIPLLSDIINCDEQFKVAI
                     ENYLEPFLSHFIVQSNEQAWNGLNKLSSEGKGRAHFFILDSFTNYHQASTHQIDGCIA
                     ASSVIENDGRYHSLMNYLLANVYIAQNSDVATQANQNEIEGKIILSPDGKFYRQRYSI
                     SGGSVGSYDGKRTGRLKHLEKLSAEIQQHQQQTEVFKIELQQTEELLSAGKTQAAENR
                     KIISLTDSELSKAVLNINTLLSKKDFITSNCESNEKNLQKLQEELQLTQEQKNNAPGF
                     SSMSLDEMKINLQKLTDQQREKQNQSGQLQKISQEASNAFNQKNITFLQQQNKVQNII
                     REIGYKNNQVVSLKSSVEKIKTEIEQLHEQINDTAQAGNSSETDLKTQVEEKVLLESR
                     LGESEKNYFEAKGKIDQFDKQIAETRRKREMADELMHALHNEVSDVNLKMTSLKERLS
                     VEFSLEVEDIVSQEANPEWNEEDLKARNEKLGGQLKNFGPINPMALDSFKEIKERYDF
                     IIKEQDDLKKAKDALLQTIAEIDNSAKEKFSEVFNQVRNNFINVFRSLFTADDNCDMI
                     LADTNNPLESDIEIIAQPKGKKPLSIQQLSGGERTLTATALLFGLYLVKPAPFCIFDE
                     VDAPLDDNNIDKFNNIIKKFSKDSQFIIITHNKKTMAATDIIYGITMNEPGVTAVVPV
                     DLREYDETLVE"
     gene            complement(2099..2350)
                     /locus_tag="PK134_06640"
     CDS             complement(2099..2350)
                     /locus_tag="PK134_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38559.1"
                     /translation="MKKFLNRFLMLLMLVISTSVVFAQSKATKAQRKAEKKKEMEKKK
                     VARAEEKARKRHMSIQTKEVRKRMKKNKKKSARETFRRE"
     gene            2423..2845
                     /locus_tag="PK134_06645"
     CDS             2423..2845
                     /locus_tag="PK134_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38560.1"
                     /translation="MRLKNIILISTLIFLISGCRKKDNGIVPYAYVNVVFYANDPQLV
                     QLNAVGNYIYYPAGYKGIIIYRRSINEFVAYERACTYDAESSCEGVTVMNDNFTLRDS
                     CCGSKFMIYDGSISQGPATHALVQYATFYDGTILRITN"
     gene            complement(2857..>3233)
                     /locus_tag="PK134_06650"
     CDS             complement(2857..>3233)
                     /locus_tag="PK134_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRB38561.1"
                     /translation="VIDKPANEVFEFLSNCNNLQQLMPEQVTNWQSTTDDCSFTIAGM
                     ASLGMEIKERNPGQNIRVERKGKAPFDFVMNYEMQTVDAGKTKLKIAFDADLNPMLKL
                     MAEKPLRNFLNTLAGNYKNLNQ"
BASE COUNT         1107 a          563 c          593 g          970 t
ORIGIN      
        1 caagaaatct tgccgaaaaa atggaaggct ttcctgagtc aattaagttc ttaaagaaaa
       61 acagcgattc gtttaaagat attccattac taagtgatat tatcaattgc gatgagcaat
      121 ttaaagttgc aatagaaaat tatttagaac ctttcctgag tcattttatt gttcagtcga
      181 acgagcaagc atggaacgga ctcaacaaac taagttcaga aggtaaaggt cgtgcacatt
      241 ttttcatttt agattctttt acaaattatc atcaggcatc aactcatcaa attgacggtt
      301 gtattgctgc atcatctgta attgaaaatg atggacgcta tcattctctg atgaattacc
      361 ttttagccaa cgtatatatt gcacaaaaca gcgatgtagc tacacaagca aatcaaaatg
      421 aaattgaagg aaaaataatt ctttcacctg acggtaaatt ttacagacaa cgttattcta
      481 tcagtggtgg ttctgtaggc agttatgatg gcaaacgaac aggacgactg aaacaccttg
      541 aaaagctatc agcagaaata caacagcatc aacaacaaac agaagtattc aagattgaat
      601 tgcagcaaac tgaagagttg ttatcagccg gtaaaacaca agccgcagaa aaccgtaaaa
      661 taatttccct tacagatagt gaacttagca aggcagtatt aaacataaat accttgttaa
      721 gtaaaaaaga tttcattact tctaattgtg agagtaatga aaagaatctc cagaaattgc
      781 aagaagaact ccagttaaca caagagcaaa aaaataatgc tcccgggttt agcagtatgt
      841 cgcttgatga gatgaaaata aatctgcaaa aacttaccga tcagcaaaga gaaaagcaaa
      901 accagtcagg gcaattacaa aaaatatcgc aggaagcctc caatgcattc aatcaaaaaa
      961 acatcacttt tcttcagcaa cagaacaaag tacaaaacat cattcgcgaa attggctaca
     1021 aaaacaatca ggtagtttct ttaaagtcat cagtagaaaa aatcaaaact gaaatagaac
     1081 aacttcatga gcagataaat gatacagcac aggcaggtaa ctcttctgaa actgacttaa
     1141 aaacgcaggt agaagaaaaa gtattgcttg aaagtcgctt aggagaaagt gagaaaaatt
     1201 attttgaagc aaagggcaag attgatcagt ttgacaaaca aattgcagaa accagacgca
     1261 agcgcgagat ggcagatgag ttgatgcatg ctttacacaa tgaagttagc gatgtgaatc
     1321 tgaaaatgac ttcattgaaa gaaagattga gtgtagaatt ttcacttgaa gttgaagata
     1381 ttgtgtcgca ggaggctaat ccggaatgga atgaagaaga tttaaaggca aggaatgaaa
     1441 aattaggtgg tcagttgaag aacttcggac cgattaatcc aatggctctt gattcgttta
     1501 aagaaattaa ggaacgttat gatttcatca taaaagagca ggatgattta aaaaaggcaa
     1561 aagacgcttt actgcaaacc attgcagaga ttgacaattc tgctaaagag aaattcagtg
     1621 aagtgtttaa tcaggttcgt aataacttta tcaatgtttt caggtcattg tttactgctg
     1681 atgacaattg cgatatgatt ttggcagaca ctaacaatcc tttagaatca gatattgaaa
     1741 ttattgcaca acctaaaggc aaaaagccac tttcaattca gcaactttca ggaggagaaa
     1801 gaacacttac ggcaacagct ttgctctttg gattgtattt agtaaagcct gcaccttttt
     1861 gtatcttcga tgaggttgat gcgcctcttg acgataataa cattgataaa ttcaataata
     1921 tcatcaagaa gttttctaaa gattcgcagt ttattatcat cacacacaat aaaaagacaa
     1981 tggctgcaac ggatattatt tatggaataa ccatgaatga gcccggagtt actgcagttg
     2041 ttcctgttga tttacgagag tatgatgaga ctttagtaga gtaaactgat aaaaaaaatt
     2101 actcgcgtct gaaagtttcg cgcgctgatt tctttttgtt cttcttcata cgtttgcgca
     2161 cttccttagt ctgaatactc atgtggcgtt tacgcgcctt ctcctctgcc ctggcaactt
     2221 tcttcttttc catttccttt ttcttttcag cctttcgttg cgccttggtt gctttgcttt
     2281 gtgcaaacac aacagatgta cttataacaa gcatcaacaa catcagaaat ctattcagaa
     2341 actttttcat attaactaaa cttcagtttt aacgattgta gaacaattat gttgtaattt
     2401 tggaagatta acttcaaagg taatgcgttt aaagaatata attctgattt caactctaat
     2461 ttttttaata tccggctgcc gtaaaaaaga caatggcatt gtgccttatg cttatgtcaa
     2521 tgtagttttt tatgccaacg acccacaatt agtccaactg aatgcagttg gtaactatat
     2581 ttattatccg gcaggataca agggaattat tatttacaga cgcagtatta acgaatttgt
     2641 ggcctacgag cgtgcctgca cctacgatgc agagagcagt tgcgaaggtg tgactgtaat
     2701 gaacgataat tttacattac gtgacagttg ctgtggttca aagtttatga tttatgatgg
     2761 aagcatttcg caaggacctg ctacacatgc attggtgcag tatgctacat tttatgatgg
     2821 cacaatactg cgtatcacga attaattgaa ggaatattac tgatttaagt ttttataatt
     2881 ccctgcaagt gtattcagga aattacgaag tggcttttct gccatcaact ttaacatagg
     2941 attcaaatct gcatcaaaag caatttttag ttttgttttg ccggcatcaa cagtttgcat
     3001 ctcataattc atcacaaaat cgaatggtgc ttttccttta cgttcaactc taatattctg
     3061 accgggattt ctttctttga tttccatgcc aagacttgcc attcctgcaa ttgtaaaact
     3121 acaatcatca gttgtacttt gccagtttgt tacctgttca ggcattaatt gctgaaggtt
     3181 gttacaatta cttaaaaatt caaaaacttc atttgcaggt ttgtctatta ccc
//
LOCUS       DAORQT010000185         3198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5837_length_3198_cov_4.776965, whole genome shotgun sequence.
ACCESSION   DAORQT010000185 DAORQT010000000
VERSION     DAORQT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_3198_cov_4.776965"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK134_06655"
     CDS             <1..378
                     /locus_tag="PK134_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRB38562.1"
                     /translation="VGALEQCRKKNIATGCIVCNSGSPVAMHADYPIEIITGPEFVTG
                     STRMKAGTAQKLVLNMISTAVMIKLGHVKGNKMVDMQLTNNKLVSRGIRMIMDELGVP
                     EKKATLMLKKYGSVRAAIKNHKS"
     assembly_gap    572..585
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <586..999
                     /locus_tag="PK134_06660"
     CDS             <586..999
                     /locus_tag="PK134_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38563.1"
                     /translation="PTLYIKIIYCDYEKSFAIIELMGEWNDAINNDIMTLKRDVIENL
                     MEHGISHFILIGENVLNFHNSDDCYYQEWFEEVNETGWIAAVNFRDHVLSEMQRANID
                     YYLLFGGELDNLNWRTYRPSQLMAKIDSIIRHRIG"
     gene            complement(1004..1378)
                     /locus_tag="PK134_06665"
     CDS             complement(1004..1378)
                     /locus_tag="PK134_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HRB38564.1"
                     /translation="MSRFKKFFIWVVVLAIIGTALYFLAGSYTYSEGTQTGYLVKFSR
                     QGYVFKTYEGELNLGGMNAESNSVVNNLWHFSVDEDDKAAIDSLRVSEGRVIKVYYRE
                     IVRNYAWQGETPFFVYKVEKVK"
     gene            complement(1375..1626)
                     /locus_tag="PK134_06670"
     CDS             complement(1375..1626)
                     /locus_tag="PK134_06670"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRB38565.1"
                     /translation="MWQEVNNSLERTFIFNDFKQAFAFMTHVAFIAEKMNHHPDWSNV
                     YNKVTIKLNTHDAGSVVTEKDHKLAAAIDVVFNQINKKV"
     gene            complement(1673..>2608)
                     /locus_tag="PK134_06675"
     CDS             complement(1673..>2608)
                     /locus_tag="PK134_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HRB38566.1"
                     /translation="HTLITSHTAMTMLCSDDKHPDELLHGHLNQLVVRALNKGHNLFD
                     VLQCACVNPVLHYSLSVGLLRVGDPADFIVINNPEVFLVSQTWINGECVANDGQTTLT
                     NRSHAPINNFNAWDVSVQDIEVKKNSESVKVIEALDGQLITNCIQAKPLEKDGLWVSN
                     PENDILKIAVVNRYSKQKPVVGFIKNFGLKLGAIASTVAHDSHNIIVTGVSDADIVDA
                     VNLLMQSGGGLSAVSGTDKKVIALPVAGLMSDKSCEEIGRDYASIDSMAKQMGSALRA
                     PFMTLSFMALLVIPHFKIGDKGLFNAVKFELTSLE"
     assembly_gap    2609..2633
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2634..>3198)
                     /locus_tag="PK134_06680"
     CDS             complement(<2634..>3198)
                     /locus_tag="PK134_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB38567.1"
                     /translation="QYLLPGFTDAHIHIESSMLVPYEFAKVALLHGTVATVSDPHEIA
                     NVCGLKGVEYMIENAKDAKLKFNFGAPSCVPATTFETAGAVLNAEDVAQLLARPDIRY
                     LSEMMNYPGVLFGDIEVMKKIESAHRLNKVIDGHAPGLRGDDARKYIAAGISTDHECF
                     TLEEALDKVNGGMKILIREGSAARNFEA"
BASE COUNT          985 a          632 c          557 g          985 t
ORIGIN      
        1 gtaggtgctc ttgaacaatg ccgcaagaaa aatattgcta caggatgcat tgtttgtaat
       61 agcggctctc ctgttgccat gcatgctgat tatcctatag aaattattac agggcctgaa
      121 tttgtaaccg gcagcactcg catgaaagca ggaactgcac agaagctagt tctgaatatg
      181 ataagtacgg cagtgatgat aaaacttggt catgttaaag gcaataaaat ggttgacatg
      241 cagcttacca ataataaatt agtcagccgt ggcatcagaa tgataatgga tgaacttggc
      301 gtacccgaaa aaaaagctac tctgatgctg aaaaaatacg gcagcgtgcg ggcagcaata
      361 aaaaatcata aatcataaac tgttgtagtt tgaatttgtc tgttgtaatt ttgcatttta
      421 ttatcaaaca atgcaggata tacagccttt ttacaactgg gtagatctat actcatctgc
      481 agaagatgag ttgagtcctt tttatggaag ggtttacagt gagtttgaat acagcaatac
      541 catttacaac tattttatcc atccgcagtg gnnnnnnnnn nnnnnccaac gctttatata
      601 aaaattattt attgcgacta tgaaaagtca tttgccatta ttgaattgat gggcgaatgg
      661 aacgatgcca tcaataatga catcatgacc ttaaaaagag atgttataga aaatctgatg
      721 gagcatggta taagccattt tattcttatt ggtgaaaacg tactcaactt tcacaattca
      781 gacgattgtt actatcagga atggtttgaa gaagtgaatg aaacaggatg gattgctgct
      841 gtcaatttca gagatcatgt tttgtctgaa atgcaacgtg ccaatattga ttactattta
      901 ttgtttggcg gtgaacttga taatcttaac tggcgcacct acaggccttc gcaactaatg
      961 gctaaaatag attctattat caggcatcgt ataggataaa aacctatttt actttttcta
     1021 ctttgtaaac aaaaaaaggt gtctcgccct gccatgcata attgcgtaca atctcacggt
     1081 aataaacttt aatcacacgc ccttcgctga cacgaagact gtcaatagca gccttatcat
     1141 cttcgtcaac agaaaaatgc cagaggttat ttacaacaga attgctttcg gcattcatac
     1201 caccaagatt tagttcacct tcataagttt taaaaacata accctgacgt gaaaatttta
     1261 ctaagtagcc tgtttgtgtt ccttcggaat aggtataact tcctgcaaga aaatacaatg
     1321 ctgttccgat tatggcaaga acaactaccc atataaaaaa ctttttaaaa cggctcatac
     1381 ttttttattt atttgattga acacaacatc tattgctgct gcaagtttat gatctttttc
     1441 tgtaaccaca cttcctgcat cgtgtgtgtt taacttaata gtgactttat tataaacatt
     1501 gctccagtcg ggatgatgat tcattttctc agcaataaaa gctacatgtg tcataaatgc
     1561 aaatgcttgt ttaaaatcat taaaaataaa tgttcgttcc aatgaattat ttacttcttg
     1621 ccacatacat tttttttgta aaagtagtac ttcattgcta aacacaaata aattattcca
     1681 gtgaagttag ttcaaacttt acagcattga ataatccttt atcgccaatt ttaaaatgtg
     1741 gaattaccaa taaagccata aatgatagtg tcataaaagg tgctcttagt gcagatccca
     1801 tttgttttgc catactatca atacttgcat aatcgcgacc aatttcttcg caacttttat
     1861 cactcatcag tcctgctaca ggcaaggcta taactttttt gtctgttcct gatacagcac
     1921 tcagtcctcc acccgattgc ataagtaaat ttacagcatc aacaatatca gcatcactaa
     1981 caccggtgac aattatattg tgcgagtcat gtgcaacggt tgatgcaata gctcctaact
     2041 ttaatccaaa atttttaata aatcctacaa caggcttttg tttgctataa cggttaacga
     2101 ctgcaatttt aagaatatcg ttttccggat tgctaaccca cagtccgtct ttttctaatg
     2161 gctttgcctg aatgcagttg gtaattaact gcccatccaa tgcttctata actttaaccg
     2221 attcggaatt ttttttcact tcaatatcct gaaccgaaac atcccatgca ttaaaattat
     2281 ttattggagc atgactacgg tttgtcaatg ttgtttggcc atcattggca acacattctc
     2341 catttatcca tgtttgcgag actaaaaaca cttccgggtt attgataacg ataaaatctg
     2401 ccggatcacc aacacgaagt aatcctacgg ataaactata atgcaacaca ggattaacac
     2461 aagcacattg caaaacatca aaaagattgt gtcctttatt gagagcgcgg acaactaact
     2521 gattcaggtg tccatgaagc aattcatcag gatgtttatc atcgctacac aacatagtca
     2581 ttgctgtatg tgaagtaatg agcgtatgnn nnnnnnnnnn nnnnnnnnnn nnnggcttca
     2641 aaattccgtg cagcagagcc ttcacgtatc agaattttca ttcctccatt taccttatcc
     2701 aatgcttctt caagcgtaaa acattcatgg tcggtagata tgcctgcagc aatatacttt
     2761 cgggcatcat cacctcttaa accgggtgca tgaccatcaa tcactttatt cagtctgtga
     2821 gccgactcaa tttttttcat cacctctata tcgccaaaaa gtacaccggg gtagttcatc
     2881 atttcgctca gatagcgaat atcgggtcgt gcaagaagtt gtgccacatc ttcggcatta
     2941 agcactgcac ctgctgtttc aaaagtggtg gcaggcacgc atgatggtgc accaaaatta
     3001 aatttcaact tggcatcttt tgcattctca atcatatatt ccacaccttt gaggccacaa
     3061 acattggcaa tttcatgcgg gtcgctaaca gttgctacgg tgccatgaag cagggctact
     3121 ttagcaaatt cataaggcac aagcatgctg ctttcaatat gtatatgggc atcggtaaaa
     3181 ccgggtagta aatattgg
//
LOCUS       DAORQT010000186         3161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5941_length_3161_cov_10.030586, whole genome shotgun sequence.
ACCESSION   DAORQT010000186 DAORQT010000000
VERSION     DAORQT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_3161_cov_10.030586"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK134_06685"
     CDS             complement(<1..269)
                     /locus_tag="PK134_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase"
                     /protein_id="HRB38568.1"
                     /translation="MLVKKLFLILFSVATTLTCAASVGDTTFVSSHTNDTVVTNPNTG
                     SNGYPNWVVFPSASTNYRKIILTLTHRCPSPMSCGEWDYLDYIYI"
     gene            472..1695
                     /locus_tag="PK134_06690"
     CDS             472..1695
                     /locus_tag="PK134_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRB38569.1"
                     /translation="MKKFISGFSILFLFCSLHVSGGGFQMQPQSHSSAGMAGAVIALQ
                     TDGSVIFYNPGGMVFLEKSNVFGGISYSQSRMAFRSNNSGLVNRTESKNMLPFYVGGT
                     IMLSEKMAAGIVVNTPFGYNTTWKSDWEGRNIILSAKLNTIFIQPTFSYKISEKLGIG
                     AGFVFASANYKWNRALIVADGADAQLDGKATGFGFNAGINYAINKKLTAALAYRSKVK
                     LNFKNTDVTFSNIPASLTNQYPASANAAAEMPLPSVLSIGFSYEVIKGLKLSFESNLN
                     GWSSFDSLNYEFDAPNTALNQEFRQARKYRDALTLRLGANYKISDKVTGRAGIAFDQT
                     PVKENYMTPDMPDGDRYVFGIGASFNVSKTFVIEGSYNFETKAEREGVNKDTGLNGAF
                     NALSHCFGVGVNYSF"
     gene            1723..3075
                     /locus_tag="PK134_06695"
     CDS             1723..3075
                     /locus_tag="PK134_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38570.1"
                     /translation="MSNNHILKSLLLAVIMIAGCKPELKELKFDNNSIDFSKYVAIGS
                     SYTAGYQNNALFDMGQQNSFAAILASQLKKVGGGEFLQPLVSGNDGAGFNFFLQTYIP
                     QTKLGYGTTCSSAPGFSTFPSNVTDIVAVETYIGNNAPYNNMGVPGMKSSDLDNANFG
                     STDLLLSKNPFYARFATNPETSTVLTDAVRQLPTFFSFWVGFNDIYDCAMAGSNQTLA
                     TITPLANFQNNINNALTSLIAGNRKGVVANIPDLLDLPYFTSIAYDALVLTEQQAQDL
                     NALYASNNPPITFQAGNNPLVIADPSAASGKRQMKPGELVLLPVTLDSINCAGWGSNT
                     QKPLGNRYVLTTSEINTLRNTITAYNNYLKTKADENNLAYVDMYAFFKKINNGFPHNG
                     VTYSSEFLKGQFFSLDGITPTGRGSALIANEFIKAINEKYQTRIPQTDANSFPGILFP
                     "
BASE COUNT          989 a          602 c          609 g          961 t
ORIGIN      
        1 ctgatgtaaa tataatcgag ataatcccat tcgccacatg acatagggct tgggcaacga
       61 tgtgttaaag ttaaaattat cttacgatag ttggtggatg cagaaggaaa tactacccaa
      121 tttggataac cattgctacc tgtgttggga ttggttacta cagtgtcatt ggtatgcgag
      181 cttacaaagg ttgtgtcgcc aacagatgct gcacaagtta aagttgttgc aacagaaaat
      241 aatatcagaa ataacttctt gactaacatg tgaaatgatt ttggttggtg aaaaaatgta
      301 ttacaattac agtgcaattt taatacaaaa aatccgaata ttggttgttg gagtgcaaac
      361 attttttaga aactgcacca acaaatcggt gttaacaaca aattgtcatc gttatgtata
      421 tctttgctta ctaagcttgc tgttcttatt tttgtttgcg ttaaaattaa tatgaaaaaa
      481 ttcatttcag gtttttcaat tctgtttctt ttctgtagtc ttcatgtatc aggcggtggt
      541 tttcagatgc aaccacaaag ccatagttca gcaggtatgg ccggtgcagt gattgcattg
      601 caaactgatg ggtcagttat attttacaat cccggtggca tggtttttct tgagaaaagt
      661 aatgtctttg gtggtatttc ttattcgcag tcaagaatgg ctttccgtag taacaacagc
      721 ggattggtaa accgtacaga atcaaaaaat atgctgccgt tttatgtagg tggcaccata
      781 atgttatctg aaaaaatggc tgcaggtatt gtagtcaata cgccttttgg atacaacaca
      841 acctggaaaa gcgattggga agggcgaaat attatactgt cagctaagct gaacaccata
      901 tttattcaac ctacattctc ttataaaatt tctgaaaaat taggaattgg tgcaggcttt
      961 gtttttgcca gtgccaacta taaatggaat cgtgcgttga tagttgcaga tggagcagat
     1021 gcacaacttg atggtaaggc aacaggcttt ggttttaatg caggaataaa ttatgccatc
     1081 aataaaaaat taactgctgc acttgcatac cggtcaaaag ttaaactcaa ctttaaaaac
     1141 actgatgtta cctttagtaa tattccggca tcgttaacga atcaatatcc tgcctcagca
     1201 aatgccgctg cagaaatgcc gctgccttct gttttgtcaa ttggtttcag ctatgaagtc
     1261 attaaaggat taaaacttag ttttgaatct aacctcaacg gttggtcgtc attcgattct
     1321 ctgaattatg aatttgatgc accaaacaca gcattaaatc aagaatttcg tcaggcacgc
     1381 aaatacagag atgcattaac acttagatta ggtgcgaatt ataaaatcag cgataaagtt
     1441 acaggtcgtg caggtattgc cttcgatcag actccggtaa aagaaaacta catgacccct
     1501 gatatgcccg atggcgacag atatgttttt ggtattggtg catcatttaa cgtgagtaaa
     1561 acttttgtta ttgaagggtc atacaatttt gaaactaagg ccgagcgcga aggtgtaaat
     1621 aaagataccg gattgaatgg tgcattcaat gcactgtcgc attgttttgg tgttggtgtc
     1681 aactattcat tttaattaaa taataaacca caatataaac gcatgagcaa taatcatatt
     1741 ctgaaaagtt tacttcttgc agtaatcatg attgcaggtt gcaagcctga gttgaaagag
     1801 ctgaagttcg acaacaacag tattgacttt tcgaagtatg ttgctattgg cagttcttac
     1861 acagcgggct atcagaacaa tgcattgttt gacatggggc aacaaaatag ttttgcagcc
     1921 atacttgcct cacaattaaa aaaagttggc ggtggcgagt ttctgcaacc attggttagc
     1981 ggaaatgatg gtgcagggtt caactttttt cttcagacct atattccaca gacaaaactt
     2041 ggctatggca caacatgcag ttctgctccg ggcttctcta catttccatc caacgtaact
     2101 gacattgtcg cagtagaaac ttacatcggc aacaatgcac cttacaacaa catgggtgtg
     2161 ccgggtatga aatcgtctga tttagacaat gccaattttg gctctacaga tttactgctt
     2221 tcaaaaaatc ctttctacgc acgatttgct accaaccctg aaacatcaac agtacttaca
     2281 gatgcagtca gacaactacc cacgttcttt tctttttggg ttggctttaa cgacatttat
     2341 gattgtgcaa tggcaggcag caatcagaca ttggctacca ttacaccatt agctaatttt
     2401 caaaataaca tcaataatgc tcttacatca ctcattgcag gaaatcgcaa aggtgtagtt
     2461 gcaaatattc ctgacctact cgacctaccc tattttactt ccatagccta cgatgctctg
     2521 gtacttacag aacaacaggc tcaggatctt aatgcattat atgcttcaaa caatccaccg
     2581 ataacttttc aggcaggtaa caatccattg gtaattgctg acccatctgc agcatcagga
     2641 aaaagacaaa tgaagccggg tgagttagtg ctgttgccgg ttacccttga cagtataaac
     2701 tgtgcaggtt ggggcagcaa tacacagaag cctcttggaa acagatatgt gctcaccact
     2761 tcagaaatca atacgcttag aaatacaatc accgcctaca acaattatct taaaacaaaa
     2821 gctgatgaaa acaatttagc ttatgttgat atgtatgcct tctttaaaaa aatcaacaat
     2881 ggttttccgc acaatggtgt aacttacagc agcgagtttc ttaaaggaca gtttttttct
     2941 cttgacggca ttacgcctac tggaagaggc agtgcactta ttgccaatga atttataaaa
     3001 gctattaacg aaaaatatca gacacgcatt ccacaaacag atgcaaattc gtttcccgga
     3061 attttatttc cgtaaggtgt tttcgattaa aaattaattt tttgtaaact aaactgtctc
     3121 tgattttacc agcaaactcc cttctgcttt taaaacatca a
//
LOCUS       DAORQT010000187         3153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_5978_length_3153_cov_4.435765, whole genome shotgun sequence.
ACCESSION   DAORQT010000187 DAORQT010000000
VERSION     DAORQT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_3153_cov_4.435765"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="PK134_06700"
     CDS             <1..1920
                     /locus_tag="PK134_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38571.1"
                     /translation="VGDPSRMAANYAGVSGANDSRNDIIIRGNSPTGLLWRLNGVEIP
                     NPNHFGSLGTTGGPISILNNNVLDKSDFLTGAFPAEYGNVLAGVFDLQMRTGNNEKHE
                     FLAQVGFNGFEAGAEGPISKKQGSSYLINYRYSTLGVFNALGINFGTGTAIPKYQDVS
                     FNIKIPTNKAGKFTLFGMGGISGIEVLEKDRDTTQKNLYVDDKRQNGYFDNTMGVVGL
                     MHTFFINNSTYTKFSLSASIAGQKYKVDTLPDFVDYAVPYYRNKSDQQKYSADFSLNK
                     KFSSKDFLKTGFSFSQYHYTYRDSGVVDSVWESYTDYNGNALLTKLYVEWQHRFSDQL
                     SLNSGVYTQLFNYNNTYSIEPRIGAKWEISDKQSLSLGTGLHSQLQPMYIYFVKTMKD
                     DGSYYETNKNLKMTKSYHAVLAYDRNISKVFRIKTEVYYQYLFDAPVEKRPTFFSVLN
                     EGADFGIAGIDSLENKGTGYNYGFEFTAEKFYSKGYYFLFTASLFESKYKGSDGVERS
                     TAFNGNFVFNLLGGKEWTIRKKNTISINIKTTYAGGRRYIPIDFAASQITGETEYDED
                     HAFEHRRKDYFRTDLKIGYRINRKRLTHEIAIDLNNVFNTQNIWNQQYSAKTNTVKTE
                     YQMGFLPIPMYKCTF"
     gene            2031..>3153
                     /gene="mutL"
                     /locus_tag="PK134_06705"
     CDS             2031..>3153
                     /gene="mutL"
                     /locus_tag="PK134_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRB38572.1"
                     /translation="MADVIQLLSDTVANQIAAGEVILRPSSVIKELMENAVDAGATRI
                     DVSINDGGKNLIRITDNGCGMSETDARLCFERYATSKLRKADDLYAIRTMGFRGEAMA
                     SVAAVCQVELKTKREEDETGTRIEIEGSEFKIQEPCVMQSGTIITVKNIFFNVPARRN
                     FLKATPVEARHVMDEFLRVALPRPEIGFSLQHNGMEVYNLPATHQRQRICQVFGATYN
                     ERLVPVEEDTTILNIKGFVGKPEFAKKVRGEQYFFINKRFVKDAYLNHAVTIAFNELL
                     PQGTFASYFLFFEVDPSRIDVNIHPTKTEVKLEDEKSVYAILRSSVKRALGRFSIAPT
                     LDFNQEQAFNVPLSQLNTIPVQPQVKVNTNYNPFKSTEIN"
BASE COUNT         1038 a          582 c          633 g          900 t
ORIGIN      
        1 gttggtgatc catcgcgtat ggctgcaaac tatgcaggtg taagtggtgc caacgactcc
       61 agaaacgaca tcatcattcg aggtaattca ccaacaggat tgttatggag actcaatggt
      121 gttgaaattc cgaatcctaa ccattttggt tcattaggta ctacaggtgg ccctatttca
      181 attcttaata ataatgtttt ggacaaatct gattttctta caggtgcctt tcctgcagaa
      241 tacggaaacg ttcttgcagg tgttttcgac ctacaaatgc gaaccggcaa caatgaaaaa
      301 catgagtttc tggcacaggt tggctttaat ggatttgaag caggtgcaga agggcctatc
      361 agtaaaaaac aaggctcatc ctatttaatt aattaccgct attccacact tggagttttt
      421 aatgcattgg gaattaattt cggaacaggc actgcaatac caaaatatca ggatgtttct
      481 tttaacatta aaattccaac caacaaagcc ggaaaattca cgttattcgg catgggaggt
      541 atcagtggta ttgaagttct tgaaaaagac agagacacta ctcaaaaaaa tctttatgta
      601 gatgataaaa ggcaaaatgg ttacttcgac aataccatgg gcgttgttgg actgatgcat
      661 acatttttta tcaacaattc tacctacacc aaattcagcc tttctgcatc tattgcaggg
      721 caaaaataca aagtggacac cttgcccgac tttgttgatt acgctgtgcc ctattacaga
      781 aacaagtccg accagcagaa atacagtgct gatttcagcc ttaacaaaaa gttttcttca
      841 aaagacttcc tgaaaaccgg attcagcttt tcgcaatatc actataccta tcgcgacagt
      901 ggtgtggtgg acagtgtttg ggaatcttac acagattata acggcaatgc actactcacc
      961 aaactttatg tagaatggca gcatcgtttt tccgatcagt tatcattgaa cagtggagtt
     1021 tacacacaac tatttaatta caataatact tacagcatag aaccacgcat tggagccaaa
     1081 tgggaaatca gcgataagca atctctgagt ttgggtacag gactacacag tcagttacaa
     1141 ccaatgtaca tctattttgt aaaaacaatg aaagatgatg gcagctatta cgaaacgaac
     1201 aaaaatctaa aaatgactaa gagctatcat gccgtgttgg cttatgacag aaatatttca
     1261 aaggttttca gaataaaaac agaagtgtat tatcaatatt tatttgatgc cccagttgaa
     1321 aaacggccta catttttttc agtgttaaat gaaggtgccg actttggcat tgcaggaatt
     1381 gacagtttag aaaataaagg cacaggttat aactatggtt ttgaatttac tgctgagaaa
     1441 ttctacagta aaggttatta tttcctattt acagccagcc tctttgaatc gaaatataaa
     1501 ggctctgatg gcgttgaacg aagcactgca tttaatggca attttgtttt caatttattg
     1561 gggggaaaag aatggacaat cagaaaaaag aacaccattt caataaatat taaaacaacc
     1621 tatgccggtg ggcgcagata tattcctatt gactttgcag catcgcagat tacaggagaa
     1681 acagaatacg atgaagacca tgcatttgag catcgtagaa aagattactt ccgcaccgac
     1741 ctgaaaatag gttacaggat aaacagaaaa agactaactc atgaaattgc cattgacctg
     1801 aataatgtct tcaacacaca aaacatctgg aatcagcagt atagcgccaa aacaaacact
     1861 gtaaaaacag agtatcagat gggtttttta ccaataccga tgtataagtg tactttttga
     1921 taatggcagc taaaaatata gtacactgcc ttccaaattt tccatgaata atctttaagc
     1981 tactcactgt aattctttaa tattgacctg ccattgatta actttgccgc atggcagatg
     2041 taatacaatt gttgtccgat actgttgcca atcagattgc agcgggcgaa gttattttaa
     2101 gaccatcatc agttattaaa gaactgatgg agaatgctgt tgacgcaggc gcaacacgca
     2161 ttgatgtaag tattaacgat ggtggaaaaa acctgatacg gattacagac aatggctgtg
     2221 gcatgagtga gactgatgcg cgtctttgtt tcgagcgtta tgcaacatct aaattgcgca
     2281 aggctgatga cttatatgcc atcagaacga tggggttcag aggagaagca atggcatctg
     2341 tagctgctgt ctgtcaggtt gaattaaaga ctaaaagaga agaagatgaa accggaacac
     2401 gcatagaaat tgaaggctct gagtttaaaa ttcaggagcc atgtgtgatg cagtccggca
     2461 caattattac cgtaaaaaat attttcttta atgttcctgc aagaagaaat tttctgaaag
     2521 caacaccggt agaagcacgt catgtcatgg atgagtttct gcgggtggct ttaccgcgac
     2581 ctgaaattgg atttagtctt cagcataatg gcatggaagt ttacaacctg ccggcaacac
     2641 atcaacggca acgtatatgt caggtttttg gagcaactta taatgaaaga ttggtgcctg
     2701 tagaagaaga cacaaccatt ttaaatatta aaggatttgt aggtaaacct gagtttgcca
     2761 agaaggtcag aggtgaacag tattttttca ttaacaaacg ctttgttaaa gatgcctatc
     2821 tgaatcatgc tgtcaccatt gcattcaatg agttgttacc tcaaggcact tttgcatcgt
     2881 actttttgtt ttttgaagtt gacccttcgc gtattgatgt caacattcac ccgacaaaaa
     2941 cagaagtaaa acttgaagat gaaaaatcgg tttatgccat attgcgctct tctgtgaaac
     3001 gtgctttagg cagattcagt attgcaccta cgttagactt taatcaggaa caagctttta
     3061 atgtgccgtt atcgcagtta aacactattc ctgtacagcc tcaggtgaaa gtgaatacca
     3121 attacaatcc atttaaaagt actgagataa ata
//
LOCUS       DAORQT010000188         3144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6012_length_3144_cov_7.712528, whole genome shotgun sequence.
ACCESSION   DAORQT010000188 DAORQT010000000
VERSION     DAORQT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_3144_cov_7.712528"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK134_06710"
     CDS             complement(<1..509)
                     /locus_tag="PK134_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRB38573.1"
                     /translation="MESSKIKIKSVLQFFLKATIALFSLWFIVHKIIHRENEVSFFDF
                     VNTRLHTEGAYSLLISVIVLMVLNWSIEAIKWKVLINRLTKISFVRSLSAVFAGATVS
                     FFTPNRVGDYAGRMLFLPSSVRIASLLSTFAGNISQLIVTLCCGLAAMALSLQHYVQW
                     NESLVVTTRV"
     gene            560..>1030
                     /gene="ruvC"
                     /locus_tag="PK134_06715"
     CDS             560..>1030
                     /gene="ruvC"
                     /locus_tag="PK134_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRB38574.1"
                     /translation="MSESEKIILGIDPGTNVMGYGVLLISGTEMKLETAGIIHLEKFD
                     NHPLKLKHIFEKTLRLIESYLPDEMAIEAPFFGKNVQSMLKLGRAQGVALAAALFKNI
                     PVFEYSPKKIKQSVTGNGSATKEQVAAMLQQLLHFEEMPKYLDATDGLAAAVCHH"
     assembly_gap    1031..1040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1146..>3144)
                     /locus_tag="PK134_06720"
     CDS             complement(1146..>3144)
                     /locus_tag="PK134_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB38575.1"
                     /translation="ISPTAGQMPRLLGLALASKFYRNNPELKSDKFKNFSVDGNEVAF
                     GTIGDASTSEGLFFETINAAGVLQVPMVISIWDDGYGISVPNKYQTTKQSISEALQGF
                     ARTQEKPGYEIIRAKGWDYAELCDAYEQAANIARTEHVPVMVHIQQMTQPQGHSTSGS
                     HERYKSKERLLWEEEFDCLRKMREFMVQSAIATEQELDAIDAEAKKFVNDGKKQAWDS
                     FTSEINTEKQQALSLAEAAISKSSNGVFLNKAVEEIKNITNCERRDIAVFIQQVLFTL
                     RADNSAEIEALKQWYRQWHQLNFDRYSSQLYAEGNGSALGIAEVKAVYSAEANMVDGR
                     EVLLANHDKLFSQYPEYIAFGEDVGTIGGVNQTFAGMQAKYGEHRVFDVGIREATIAG
                     QGIGMAVRGLRPLAEIQYLDYLAYAIQILSDDLACLRYRTKAGQKAPLIVSTRGHRLE
                     GIWHSGSPMQFILGALRGMLVLVPRNMTQAAGFYNLLLQCDDPALVIEPLNGYRLKEK
                     LPENLGAFTVPVGVPEILEEGNDVTLVTYGSCVRVAQDAMKQLQQCGISVELIDVQSL
                     IPFDIHHIIAESIQKTNKVVFFDEDVPGGATAYMMQQVIENQKAFEFLETPPVTLAAK
                     EHRPAYGSDGDYFSKPNAEDVFNTVYELMHSVNPQAFPLFR"
BASE COUNT          983 a          686 c          574 g          891 t
ORIGIN      
        1 acacgtgttg tgacgactaa actttcattc cactgtacat aatgttgtaa tgacaaagcc
       61 attgcagcaa gtccacaaca aagtgtaaca atgagctgac tgatatttcc tgcaaaagtt
      121 gaaagcagtg atgctatgcg aacagatgaa ggcaaaaaaa gcattctacc tgcatagtcg
      181 ccaacacggt taggtgtaaa aaagctaaca gtagccccgg caaaaaccgc acttaggctt
      241 cgaacaaaac taattttggt gagtctattt attaagacct tccactttat agcttctata
      301 ctccaattca acaccatcaa tacaataaca gatatgagca aggaataagc accttctgta
      361 tgcaggcgag tattcacaaa atcgaaaaac gacacttcat tctcgcggtg tataatttta
      421 tgaacaatga accaaagaga aaacaatgca attgttgctt tcagaaaaaa ctgtagcaca
      481 cttttaatct ttatttttga actttccaag caattgcaga ttatagtgca agtttaaaaa
      541 ataagcggta atagcgttca tgtcagaaag cgaaaaaata attttaggaa tagatccggg
      601 cactaatgta atgggatatg gagtactcct tatttcggga acagaaatga aattagagac
      661 cgcaggtata attcatctcg aaaaatttga caaccatcca ctgaaattaa agcatatttt
      721 tgagaaaaca ttacgcctta ttgaaagcta tttgcctgat gaaatggcaa ttgaagcacc
      781 gttctttggc aagaatgtac aatctatgtt aaagttaggt agggcacaag gagttgcact
      841 tgccgctgca ttattcaaaa atattcctgt atttgaatac agcccaaaaa aaataaaaca
      901 atctgtcacc ggcaatggta gtgccaccaa ggagcaggtt gctgctatgc ttcaacagtt
      961 gttacacttt gaagaaatgc caaaatatct tgatgctacc gatggcctgg cagctgcagt
     1021 atgtcatcac nnnnnnnnnn taccgggcaa agtcagtttg cgggaggcaa aagcaaagga
     1081 tggcaaaaat ttatttctga taatcccgac aaattaatcg ggtagagaaa ctaagtttat
     1141 cctgactatc tgaacaatgg aaatgcctgt ggatttacag agtgcattaa ttcatacaca
     1201 gtattaaaca catcttccgc attaggcttc gaaaaataat ctccatcgct tccgtatgcc
     1261 ggacgatgtt cttttgcagc aagagttaca ggcggtgttt caagaaactc aaatgctttt
     1321 tgattttcaa taacctgttg catcatgtat gcagttgcac caccgggaac atcttcatca
     1381 aagaaaacaa ccttattcgt tttttgaata gattcagcga tgatatgatg aatgtcaaat
     1441 ggtatgagtg actgtacatc tatcaactct acactaatgc cacattgctg cagttgtttc
     1501 attgcatcct gagcaaccct aacacatgaa ccgtaagtta ccagagtaac atcattacct
     1561 tcttcaagaa tttcaggcac accgacaggc acagtaaaag cacctaggtt ctcagggagt
     1621 ttttctttta gtctgtagcc attcaatggt tctatcacca atgcaggatc atcacattgc
     1681 aatagcaaat tataaaatcc tgctgcctgt gtcatgttgc ggggaacaag cacaagcata
     1741 ccacgcaaag caccaagaat aaactgcata ggtgaaccag aatgccagat gccctctaac
     1801 cgatgtccac gagtactaac aatgagtggt gctttctgtc ctgccttggt gcgataacgc
     1861 aaacaagcta aatcgtctga taaaatctgt attgcataag ccaaataatc gagatactga
     1921 atttctgcaa gtggtcgcaa gcctcgcaca gccattccta ttccctgtcc tgcaattgtt
     1981 gcctcacgga tacctacatc aaaaacacga tgctcgccat acttggcctg catgcctgca
     2041 aaagtttggt taacgccacc aatcgttcca acgtcttctc caaaggcaat gtattcagga
     2101 tattgactaa aaagtttatc atgatttgca agaagtactt cacgaccatc aaccatgttt
     2161 gcctctgcag aatatacagc cttaacttcg gcaatgccca atgcgctgcc gttaccttct
     2221 gcataaagtt gtgagctgta acgatcaaaa ttcaactgat gccattggcg gtaccattgc
     2281 ttcagtgctt ctatctcagc actattatca gcacgaagcg taaacaaaac ttgctgtata
     2341 aaaacagcaa tatctcttct ttcgcaattg gtgatattct taatctcttc aactgcttta
     2401 ttcagaaaaa caccatttga acttttgctg attgctgctt ctgccagtga aagtgcctgt
     2461 tgtttttcag tattaatttc agaagtaaaa ctatcccatg cctgcttctt tccatcatta
     2521 acaaactttt tggcttcagc atctatagca tcgagttctt gttctgttgc aatggcagac
     2581 tgcaccataa actcacgcat ttttctgaga cagtcaaatt cttcttccca caacaagcgc
     2641 tctttagatt tgtaacgctc atgcgatcct gatgtagagt gaccttgcgg ctgtgtcatt
     2701 tgctgaatat gcaccataac cggcacatgc tctgtacgtg caatgttagc agcctgttcg
     2761 taagcatcac acaattctgc ataatcccat ccctttgctc taataatttc gtaaccgggt
     2821 ttttcttgag tacgagcaaa accttgtaat gcttcactga tgctttgttt tgttgtctga
     2881 tatttattgg gcacagaaat accataacca tcatcccaaa tgctgataac cattggcacc
     2941 tgcaaaacac ctgctgcatt gatggtttca aaaaacaaac cttcagaagt gctggcatca
     3001 ccaatagttc caaaagcaac ttcattacca tcaacagaaa agtttttaaa cttatccgac
     3061 ttaagttccg gattattgcg atagaattta gaagctaaag caagtccgag caatcgtggc
     3121 atttggcctg ccgttggcga tatg
//
LOCUS       DAORQT010000189         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6023_length_3141_cov_4.581659, whole genome shotgun sequence.
ACCESSION   DAORQT010000189 DAORQT010000000
VERSION     DAORQT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6023_length_3141_cov_4.581659"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK134_06725"
     CDS             <1..248
                     /locus_tag="PK134_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38576.1"
                     /translation="PKGGVVAMTNPSNGNFTLQFNVQSTAGELEVYDVMGNLVMREYV
                     APWSQYKRVNIEALAKGIYLCKLKWGSVERSVKVIKE"
     gene            477..1028
                     /locus_tag="PK134_06730"
     CDS             477..1028
                     /locus_tag="PK134_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB38577.1"
                     /translation="MSTKYKFQEPDAIYFVTFSVVNWIDVFTRNTYKDILLDSIRHCQ
                     KEKGLVVHAWVIMTNHVHMIISKNSLRVLPDIMRDLKKFTAYKNIGAIMESPQESRRE
                     WMFKLFKQAGENNSNNTNWQFWQQDNHPIELTDATMQEQRLNYIHENPVRAGFVYKAE
                     DYVYSSACDYGGTKGLLDLLLLE"
     gene            complement(1132..1467)
                     /locus_tag="PK134_06735"
     CDS             complement(1132..1467)
                     /locus_tag="PK134_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRB38578.1"
                     /translation="MSKSVDEFNDYRAKMNKRILESDNLVLKRLYNLDANTYMDGALP
                     VQTKEMLGLVASMVLRCDDCIKYHLMQCKQNGITTEQVYEVFAVANIVGGTIVIPHTR
                     RAAEFWDEI"
     gene            complement(1479..2264)
                     /gene="tatC"
                     /locus_tag="PK134_06740"
     CDS             complement(1479..2264)
                     /gene="tatC"
                     /locus_tag="PK134_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRB38579.1"
                     /translation="MNFLDHLEALRWHLVRATLAIFVLAVVAFIYKDLLFDKIIFGPK
                     NPHFITYRLLCMLSEKWSIDLCITDIPFHLISTTMAGQFTTHMVAAGIAGLILGFPYL
                     LWEIWRFVKPALSAKEKKYTRGIVFSASFLFFCGVAFGYFILAPLSINFLGSYKVSEE
                     VQNAITLDSYISIVSIMTLACGAVFELPILVFFLTKMGIISPQFMRRYRKHAFIVNLV
                     VAALITPSPDVTSQMLVAIPLFLLYEFSIFVSVVVARQESKNE"
     gene            complement(2316..>3141)
                     /locus_tag="PK134_06745"
     CDS             complement(2316..>3141)
                     /locus_tag="PK134_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRB38580.1"
                     /translation="VCTGIGKSAIIAQKITATLNSTGTPAVFMHAADAVHGDLGVIQK
                     DDVVICISKSGNTPEIKVLAPLLRLAGNKLIAMVGDTSSYLAQQAHYILNTTVAREAC
                     PHNLAPTTSTTAQLAMGDALAVCLLENRGFSSKDFARYHPGGALGKRLYLKVGDVYKN
                     NASPQVNPTDDLKKVIVEISGSRLGATAVVDKGKLKGIITDGDLRRMLHRSDDMLATK
                     AKDIMTSKPKSVSPDTMAADAFILMKSNNITQLVVTDKGKYLGFIHLHDLLKEGIY"
BASE COUNT          974 a          639 c          637 g          891 t
ORIGIN      
        1 cccctaaagg gggagttgtt gccatgacta atccgagcaa tgggaatttt accttgcaat
       61 ttaatgtgca gagtactgcc ggagaattgg aagtttatga tgtgatggga aatttggtga
      121 tgagggaata tgtggcacca tggagccagt ataagagagt gaatatagaa gcattggcaa
      181 aagggattta cctgtgcaag ttaaaatggg gaagtgttga gagaagtgta aaggttatta
      241 aagaataatg gttagtgtag aaaaagacag cagacctgag tttactttgc gtactacaac
      301 gagtcaacga attttaaata aaacaccctc tggcgcaagt cttcggactt gtgctgatct
      361 atcaataaag aatgcagtgc agtaatttta gcgtaacaaa gcagcaagca ggctaaactg
      421 gcttttaccg tgctcaaata atatttgcaa tcaatagaag ttgcaccttt gcatacatga
      481 gtaccaagta caaatttcaa gagcctgatg ccatttactt tgttactttt tcggtcgtaa
      541 actggattga tgtttttacc cgcaatactt ataaagatat tttgttggac agcattagac
      601 actgtcaaaa agaaaaaggc ttagtggttc atgcatgggt tatcatgacc aatcatgtac
      661 atatgatcat ttctaaaaac agcttgaggg tattacctga catcatgcgc gaccttaaaa
      721 agtttactgc ttataaaaat attggcgcca taatggaaag tccgcaagaa agcaggcgcg
      781 aatggatgtt taaactcttt aagcaggcag gagaaaataa ttccaacaat actaactggc
      841 agttttggca acaggataat cacccaattg aattaacgga tgcaactatg caggaacaaa
      901 gattaaatta tattcacgaa aatcctgtgc gggcgggatt tgtttataaa gcagaagact
      961 atgtttacag cagtgcatgt gattatggcg gcaccaaagg tttactggac ttactgctgc
     1021 ttgaatgata ggtgggcaca agtccgaaga cttgcgccag aggtgggttg ttaaggatta
     1081 actatttagc cgctaagcag cctatctttc gttcaggcta aaaaaagaaa tttaaatctc
     1141 atcccaaaat tctgctgctc ttctggtatg tggaatcaca atggttccac ccactatatt
     1201 ggctactgca aacacttcat aaacctgctc tgtagtaata ccattttgtt tgcattgcat
     1261 tagatggtat ttaatacaat catcacagcg cagcaccatg ctggcaacaa gcccaagcat
     1321 ttctttagtc tgaaccggca atgcaccatc catataggtg ttggcatcga gattatagag
     1381 gcgttttaaa accagattat ctgactcaag aatgcgctta ttcattttgg cacgatagtc
     1441 attgaactca tctacacttt tactcatgtg attatttttt attcgttttt actctcctgc
     1501 cttgccacaa caacagaaac aaatatgctg aactcgtata acagaaacaa aggaatagcc
     1561 accaacatct ggctggttac atctggtgat ggcgtaatta atgcagcaac aacaaggtta
     1621 acaataaaag catgtttacg gtagcgtctc ataaactgtg ggcttatgat acccattttt
     1681 gtaaggaaaa acacgagtat aggcagttca aaaaccgctc cgcaagccaa tgtcatgatg
     1741 cttacaatgc tgatatatga atcgagcgtg atagcattct gcacctcttc actcacttta
     1801 taacttccca aaaagttaat tgataatggt gccagaataa aataaccaaa agccactccg
     1861 cagaaaaaca aaaacgaagc agaaaaaact atacctcttg tatatttctt ctctttggca
     1921 gacaatgcag gtttcacaaa acgccatatc tcccaaagca aatatggaaa tcctaaaata
     1981 agtcctgcaa ttcccgcagc taccatgtgt gtggtaaact gtcctgccat ggtagtactt
     2041 atcaagtgaa aaggaatatc agtaatgcat aagtcaattg accatttctc cgaaagcata
     2101 cacagcaatc tgtaggtaat aaaatgaggg ttcttgggtc cgaaaataat tttatcgaac
     2161 aataagtcct tatatataaa tgctacaacg gctaacacaa atattgccaa tgtagcacgc
     2221 accaaatgcc agcgcaaggc ttcgaggtga tccagaaaat tcatttctga gttggggtcg
     2281 ggcttccttc tgaaaaacat ttctttattt gaagattaat atatgccttc tttaagcaaa
     2341 tcgtgaaggt gaataaaacc aagatactta cctttatcag tcacaaccaa ctgcgtaata
     2401 ttatttgact tcatcagaat aaaagcatcg gcagccatag tgtcggggct aacggatttg
     2461 ggctttgaag tcataatgtc tttagccttt gtggccaaca tatcgtcact tctgtgcaac
     2521 atcctcctaa ggtcaccatc ggtaattatt ccttttagct tgcctttatc tacaacggca
     2581 gttgccccaa ggcggcttcc cgatatttct acaatcacct ttttaaggtc atcggtggga
     2641 tttacctgcg gactggcatt atttttataa acatcgccaa ccttcagata caatcttttt
     2701 cctaatgccc cacccggatg ataacgtgca aaatctttac ttgagaaccc cctgttttcg
     2761 agcagacaca cagccaaggc atcacccatg gccaattgtg ctgtagtgct tgttgtggga
     2821 gctaagttat gcggacaagc ttcgcgggca acggtagtgt taagtatata atgtgcctgt
     2881 tgcgccagat atgaagaagt gtcgccaacc atggcgatta gtttattgcc agccaacctt
     2941 agcaaaggtg ccaggacctt tatttccgga gtgttgccac ttttggaaat acaaatcacc
     3001 acatcgtcct tctgtattac cccaaggtcg ccatgcacag catcggcagc gtgcataaac
     3061 acggctggtg ttccggtact gttgagtgtg gctgttattt tttgagctat tatggcactt
     3121 ttaccaattc ctgtacaaac t
//
LOCUS       DAORQT010000190         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6093_length_3117_cov_6.740366, whole genome shotgun sequence.
ACCESSION   DAORQT010000190 DAORQT010000000
VERSION     DAORQT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6093_length_3117_cov_6.740366"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PK134_06750"
     CDS             complement(<1..543)
                     /locus_tag="PK134_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRB38581.1"
                     /translation="MKKRIAILGSTGSIGRQALEVIAAHADAFEVDLLTANGNIELLE
                     QQVRQFTPATAVVTDKEKYKTFKENIADLPVKVFSGIESAEQAVQSDSIDIVLTAMVG
                     YAGLLPTIAAIKSKKTIALANKETLVVAGKIITSLADDNGVRIYPVDSEHSAIFQCLA
                     GEFHNPIEKIYLTASGGPFRG"
     gene            complement(655..2483)
                     /gene="glmS"
                     /locus_tag="PK134_06755"
                     /pseudo
     CDS             complement(655..2483)
                     /gene="glmS"
                     /locus_tag="PK134_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
     gene            complement(2490..>3117)
                     /locus_tag="PK134_06760"
     CDS             complement(2490..>3117)
                     /locus_tag="PK134_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRB38582.1"
                     /translation="SIINFDAKTTVSSMIMYFTDPATTGIDSLPFYIYSSDGSIARFS
                     RFTHDYSAFNFTNPLTDKAYVQTMGGIKTKISIPYLNSLKEMGPISINKAELIINVDP
                     GTATNLPAPDALSLYAIDSAGQIQTLPDLTRSSTLFGGGLANNQYRFNIAQFLQQILY
                     EKRIDYGMYLRVRVPVAFQTPNRVALVGGDPASTLRMKLQITYTKLNP"
BASE COUNT          884 a          647 c          588 g          998 t
ORIGIN      
        1 tcctctgaag ggtccacccg atgctgtcaa ataaattttt tctatagggt tgtggaactc
       61 tcctgccaga cattgaaaaa tggctgaatg ttcggagtca acagggtaaa tgcgcacacc
      121 gttatcatct gcaagtgatg ttataatttt ccctgccacc acaagggttt ctttattggc
      181 cagtgcaatg gtctttttac tttttatggc tgcaattgtt ggcagtagtc cggcataacc
      241 taccattgct gtaagaacaa tgtcaatgga gtcggactgt acagcttgct cagcagattc
      301 tattcccgaa aaaactttaa ccggaaggtc agcaatattt tctttaaacg ttttgtattt
      361 ttctttatcg gttacaacag cagtagccgg agtaaattgc cgaacctgtt gttcgagcag
      421 ttctatattg ccgtttgcag ttaatagatc aacttcaaaa gcatcggcat gtgcggcaat
      481 cacttcaagt gcctgcctgc ctatactacc ggtcgaacca agaatagcaa ttcgtttttt
      541 caaagctgat tgactatgag ttgacaatat tagttaaatc ctgttgaagt ctgtattaat
      601 caacagggat attgtaaaca gaaatatgat gagagaagta gtttaaatgc aacgttattc
      661 aacagtaaca ctctttgcca ggttacgagg ctgatcaaca ttacaaccac gcataacagc
      721 aatgtgataa ctgagcaatt gcaatggaat tacactaacc aatggtacaa gaatgtcgtc
      781 tgtttccgga atttcaatag tataatctgc catcttcctt acatcaacat cgccttctgt
      841 aacaattgca attatttttc ctttacgtgc ctttacttcc tgaatgtttg aaaccacttt
      901 ttcgtatgaa gaattttttg tggcaataac tacaacaggc atttcttcgt caatcaaagc
      961 aataggcccg tgtttcattt ctgcagcagg ataaccttct gcatgtatgt aggatatctc
     1021 tttaagcttt aatgcacctt ctaatgcaac aggaaatcca taaccacgac ctaggtaaag
     1081 gaagtttctg gagtctttaa acaatgcaga tatttcttta accttatcgt ttgatttaag
     1141 tgcaatttca acttttgaag gaatggtttc aagttcgtta agcaatgcac gaaaacgtga
     1201 ttgtgtgatg gtacctcttt tgtttgcaat ctgaagtgcc attaatgtta gtactgttac
     1261 ctgtgcagta aatgctttgg tagaagccac accaatttcg ggtccggcat gtgtgtagga
     1321 accggcatgt gttgcgcgtg gaatagaaga ccccacaaca ttacaaaccc caaaaattgt
     1381 tgcaccacgt tctttagcca tttcaatggc agcaagtgta tcggcagttt cgccactttg
     1441 cgaaatagct atcactacat catcttcaga gatgattgga tttctatagc ggaattcaga
     1501 agcatattca acctccacag gaattcgtgc caggtcttca ataagatatt ctgctaccaa
     1561 tgctgcatgc caagatgttc cgcaggcaac aatgattatt ctttttgcat tgatcaattt
     1621 gttttcaaac tcgctgatac cacctaatct caacctgcct ttgtcagcat caatgcgtcc
     1681 gcgcatgctg tcccaaatag aacgaggttg ctcatagatt tctttcatca tgaaatgatc
     1741 gtagccacct ttttcaatag cttcaagctt taactccaat tcctgaacaa aaggaatttt
     1801 ggtttcgttg tcaattgttt ttacggtaag tttaccatta tcaattgttg caacttcgcc
     1861 atcgttgagg taggcaacat ttttagtata ttctacaata ggtgttgcat cagatgcaat
     1921 aaaaaattcg tctttaccaa tacccacaac aatagggcta cccttgcggg ctgcaatcat
     1981 tttattaggc tcactctttg ataaaactac aatggcataa gctccaataa cttcgcctaa
     2041 agcaagacgt acagcctcct cagtagctac gtgtgtttta tgttttattt cttcaatatc
     2101 agcacttcag tgtctgtatc acttttaaaa gtatgacccg aatttatcag ctcttgtttt
     2161 attgctgcat agttttcgat aataccattg tggatgatgg ctaaatcttt cgattcggaa
     2221 taatgcggat gcgcatttct gtcgttgggt tcgccatgtg ttgcccaacg tgtatgtccc
     2281 atacctacct gcccaaccag gttcttatca gaaacatagt tggccaagtc ggcaacctta
     2341 cctgcttttt tgtaaacact caattcacca ttcaacaatg ccacaccggc actgtcatag
     2401 ccgcgatatt ccagccgctg aagtccttta attactattg gataagcctg acgatatcct
     2461 atataggcaa cgattccaca catgatattt tatggattta attttgtata agtaatttgt
     2521 aatttcattc gtaatgttga agcagggtcg cctcccacta aagccacacg attaggtgtt
     2581 tgaaaggcta ccggcaccct tactcttaag tacattccgt aatctatacg tttttcgtat
     2641 aaaatctgtt gcaaaaactg tgcaatatta aaacggtact gattatttgc cagtccaccg
     2701 ccaaaaagtg tagatgaacg tgtcaaatca ggcaaagttt gtatttgtcc tgcggaatct
     2761 atggcataaa gactcaatgc atcaggagca ggaaggttgg ttgccgttcc cggatcaaca
     2821 ttgatgatta attctgcttt attaattgat attggcccca tttctttcag actgttaaga
     2881 taaggaatgg aaatttttgt tttaatacca cccattgtct gaacataagc tttatctgtc
     2941 aatggattgg taaaattgaa tgctgagtaa tcgtgtgtaa accggctgaa acgggctatg
     3001 ctgccatctg agctgtaaat ataaaacggt agtgagtcaa tgcctgttgt tgccggatct
     3061 gtaaaataca ttatcattga cgaaacggtt gtttttgcat caaaattgat aatactt
//
LOCUS       DAORQT010000191         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6115_length_3111_cov_10.501309, whole genome shotgun sequence.
ACCESSION   DAORQT010000191 DAORQT010000000
VERSION     DAORQT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6115_length_3111_cov_10.501309"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PK134_06765"
     CDS             complement(<1..1245)
                     /locus_tag="PK134_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRB38583.1"
                     /translation="MSAIQEKNQSVKGGEFIIKESSAENTFIPEEFSEEHLMIEQTAN
                     DFLKQEVHTVLDRIDAMEAGLMPSLLDKAGELGLLGVSVPEQYGGFGKDFITGTLLTE
                     TLGAGHSFSVAMAAHTGIGTLPIMYFGNEKQKEKYLPLLASGQWKASYCLTEPGSGSD
                     ALAAKTKAILSSDGKHYILNGQKMWITNAGFADLFVVFAQVDGEKFTGFIVEKTYPGL
                     SLGEEEHKMGIKGSSTRQVFFSDCKVPIENVLGEIGKGHLIAFNILNIGRLKLCAAAL
                     GGAKHVSTHSVSYANTREQFKLPIAKFGAIRYKLAQQAIRIYACESALYRTAFMIDEL
                     EKQKLAEGIEYSQALLQAAEEYAVECAILKVDGSEALDYVADEGVQIYGGYGYSADFP
                     MDRSYRDSRINRIFEGTNEINRL"
     gene            1392..>3111
                     /locus_tag="PK134_06770"
     CDS             1392..>3111
                     /locus_tag="PK134_06770"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38584.1"
                     /translation="MHFTVKLAKINFLVGGNSALNKMSGMWLTALVIIFSLHQSIAQL
                     PVASFGISQSSGCAPLTVSFNNTSTNATSYHWDFGNGAVSVLNSPVITFTTAGNYYVK
                     LIATNSSGSDSVISSQPIHIENKPVVQFTLNKSSACQGEIINFTNQSAIFDSCQWDFG
                     DGVTSNQTNPTHIYATAGTYNVTLVLFRLNSTCTQTLTKNNIIIIKPIPIVTATVDTF
                     STCNINKIFHFNAGANTTSSYQWNFGDGGTATGAIVQHQYSQSGNYQVAVTASASGCS
                     STVNLSSPITVLNNPIPIITSNITSGCNPFNVVLSTNTTGTSSLLWNLDNGQTSTLST
                     VGVLYNNAGNYNPYVTANYPNGCSSQSAPLTINVLQSPVSTFTTSNTSGCKPLSISFQ
                     TTSCTGCSYQWSFGDGNSSTQLNGIHNYTQSGTFYPALTVTAANGCTATSNLPYGITV
                     NGPEADFLADKVTGCNPLTVNFANTSTGSTSWLWQFGTGDTSTVNSPTYLYQNQGSYN
                     VTLIASDGNGCKDTLRKQGYINPGPSVNNFSNPQPVSGCAPLTVSLSDSSGANSWLWD
                     YGDGSTA"
BASE COUNT          991 a          664 c          493 g          963 t
ORIGIN      
        1 gagtctgtta atttcatttg ttccttcaaa aattctgttg atgcgtgaat ctctatatga
       61 tctgtccatc ggaaaatctg cactatagcc ataaccacca taaatctgca caccttcatc
      121 tgcaacataa tcaagtgctt cagaaccatc aactttaaga atggcacatt caacagcata
      181 ttcttcggca gcctgaagta atgcctgaga atattcaatg ccttctgcaa gtttttgttt
      241 ttcgagctcg tctatcataa atgctgtacg atacaaagca ctttcacaag cataaattct
      301 gattgcctgt tgcgcaagtt tatatctgat ggcaccaaat tttgcaatag gcaatttaaa
      361 ctgctcacgt gtgtttgcat aggaaacaga gtgtgtactt acatgttttg cgccacctaa
      421 tgctgctgca cacaatttca aacgtccaat atttaaaata ttgaaagcaa taagatgtcc
      481 tttgccaatt tcgcccaaaa cattttctat aggcacttta caatcactga aaaaaacctg
      541 tcttgtactt gagcctttta ttcccatctt atgttcttct tcgccaagtg atagtcccgg
      601 ataagttttc tctacaataa aaccggtgaa cttttcgcca tcaacctgtg caaatactac
      661 aaacaaatct gcaaagcctg cattggtaat ccacattttt tgtccgttaa ggatataatg
      721 ctttccatca gaagaaagaa tagctttagt tttcgctgca agcgcatcag aaccggaacc
      781 gggttcagtt aaacagtaag atgctttcca ctgacctgat gctaatagcg gtaaatattt
      841 ttctttctgc ttttcatttc cgaaatacat tattggcaat gttccgatac ctgtatgtgc
      901 tgccattgca acagaaaatg aatgacctgc tcctaaagtt tctgtcagta atgtgcctgt
      961 aataaaatct tttccaaatc caccatattg ttccggaacc gaaacaccaa gaagtccaag
     1021 ttctcctgcc ttatctaaca atgatggcat aagtcctgct tccattgcat caatacgatc
     1081 gagtactgta tgtacttcct gttttaaaaa atcatttgct gtttgctcaa tcatcaaatg
     1141 ctcttcgcta aactcttcgg gtataaaagt gttctctgca cttgattctt taataataaa
     1201 ttctcccccc ttaaccgatt ggttcttttc ctgaattgct gacataataa aagtggtttt
     1261 tttaacgcaa taaaaataaa aatattaaac ataaatacca gccttaaggt ttcaatattt
     1321 ctaaaatacc ggaaattaaa tctactgaaa aacaaacaga taatattaaa gtgtaagaat
     1381 tataactttt aatgcacttt accgtaaaac ttgctaaaat caacttttta gtgggaggaa
     1441 atagtgcttt aaataaaatg tcaggaatgt ggcttactgc cttagtgatt attttttcgc
     1501 tacatcaaag tattgcacaa ctaccggttg catcatttgg catcagtcag tcatcaggtt
     1561 gtgcacctct aactgtttca tttaacaata cgagtaccaa tgcaacaagc tatcattggg
     1621 attttggcaa tggtgcagtt tctgttttga atagtcctgt aattactttt actactgcag
     1681 gaaattatta tgtaaaactc atcgcaacaa attcttcagg cagtgattct gtaatttctt
     1741 cgcaacctat acatatagaa aacaaacctg ttgtacaatt tacactcaat aaatcttcag
     1801 catgtcaggg tgaaattata aactttacca atcaaagtgc aatttttgac agttgtcaat
     1861 gggattttgg tgatggtgtt acaagcaatc aaacgaatcc tacacatatt tatgcaacag
     1921 ccggaactta taatgttact ttagtcttgt tcaggctcaa ctcaacatgc acacaaacac
     1981 tgacgaaaaa taacatcatt attattaagc ctattccaat tgtaacagca acggttgaca
     2041 cattcagcac atgtaatatc aataaaatat ttcattttaa tgctggtgca aataccacat
     2101 catcttatca atggaatttt ggcgatggag gaactgcaac aggcgcaatt gttcaacatc
     2161 aatattcaca atccggaaac tatcaggttg cagttactgc atcagcctcc ggttgctcat
     2221 ctaccgtaaa tctatcttca ccgataacag tacttaacaa tcctataccc ataattacat
     2281 ccaatatcac atcgggttgt aatccgttta atgttgtttt atcaactaat actacaggaa
     2341 catcatccct tctatggaac ttagacaatg gccagacatc tacactatca acagttggag
     2401 tattatataa caatgcagga aactacaatc cttatgtaac cgcaaactat cctaacggct
     2461 gttcaagcca gtctgcacca ttaacaataa atgtattaca gtctccagtc tcaacattca
     2521 ctacatcgaa tacttcagga tgtaaaccat tgagtatttc atttcaaact acatcatgca
     2581 caggatgtag ctatcaatgg agttttggtg atggcaatag ctcaacacaa ttaaatggca
     2641 ttcataatta cacacaatca gggacattct atcctgcgct aaccgttact gctgcaaacg
     2701 gatgtacagc tacatcaaat cttccctatg gaattacggt caacggacct gaagccgatt
     2761 ttcttgcaga taaagtaact ggatgtaatc cattaactgt aaactttgca aatacaagta
     2821 caggttctac ttcatggctt tggcagtttg gcacaggaga tacttcaaca gtaaacagtc
     2881 caacctatct ctatcaaaat caaggcagtt ataatgtgac tctaattgct tctgatggca
     2941 atggttgtaa agacacatta cgtaaacaag gttacatcaa ccccggacca agtgtaaaca
     3001 acttcagcaa tccgcaaccc gtatcaggat gcgcaccact tactgtttca ttaagcgact
     3061 catcaggtgc aaactcatgg ctttgggatt atggtgatgg cagtactgcc a
//
LOCUS       DAORQT010000192         3104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6148_length_3104_cov_6.613316, whole genome shotgun sequence.
ACCESSION   DAORQT010000192 DAORQT010000000
VERSION     DAORQT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6148_length_3104_cov_6.613316"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PK134_06775"
     CDS             <1..592
                     /locus_tag="PK134_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRB38585.1"
                     /translation="DNERKRVASELHDDLGAGLSTIRLLSELALQKTEGKTEIKRISE
                     LSNELVDSMRQIIWSMNPESSSLSDFIHYLKRYTMEIIELNGKIFNFKLNQEISDYTL
                     SPEQKRNLFLIIKETLHNSIKHSKSNEIILIYDYIEKRHHLTIKDNGIGFSPDDINYK
                     NGNGLINIQKRAMQIGCSVFVTSEITKGTITRIEFS"
     gene            638..1252
                     /locus_tag="PK134_06780"
     CDS             638..1252
                     /locus_tag="PK134_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB38586.1"
                     /translation="MIKVCIVEDIHEIRDGFKSVLDLSGEFKCCGTFGNAEDAIDKIP
                     ELNPEIVIMDINLPGINGIEAIKYLIKNNCNPLFLIFTVYDDDTIIFDALQAGASGYL
                     LKKTTPAQFLEAVKEMYDGGSPMSPIIARKVINSFKPGQKTNAILSVRESEILTLLAK
                     GLLYKEISEQLFISTGTVKQHIHKIYEKLHVQNRTEAINKFLNK"
     gene            complement(1450..2865)
                     /locus_tag="PK134_06785"
     CDS             complement(1450..2865)
                     /locus_tag="PK134_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRB38587.1"
                     /translation="MATKIKFGTDGWRAIIAQDFTVENVTRVAEATALWLKAKGGNPS
                     VVVGNDCRFAGNLFAETTAKVLCANGIKVMLAPDFVSTPMVSLGTLHYKADLGIILTA
                     SHNPPSYNGFKLKGSYGGPLLPAQVSEIEDMIKDASTIDTLKLSLEDFTAKGLLQIVD
                     LETMYINKVKENFDLDAIKKSGINWAYDAMYGAGQNVMKKLFPEITLLHCDHNPGFHG
                     QAPEPIHKNLTEFSELIKKRGDIGCGLVTDGDADRIGLYDDKGNFVDSHHIILLLIHY
                     LHKYKGMSGKVCTAFSTTPKVQTLCKHYGLPIDVVKIGFKYICEIMIKEDVLLGGEES
                     GGIAIKGHIPERDGIWMGLVIWEFMAKSGKSLNELIKEVYEITGPFSFERNDLHIKEE
                     VKQKILANCLQNKYAQFGNYKVQRVDDLDGFKYFFDNTNSEWLMIRASGTEPVLRTYA
                     ESSSREKAFQILDACKQTLLG"
     gene            complement(2881..>3104)
                     /locus_tag="PK134_06790"
     CDS             complement(2881..>3104)
                     /locus_tag="PK134_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38588.1"
                     /translation="NDSALNSIPLSEMTMMDLEINGQKLVLALLDKKFNFTITKINSQ
                     DIALRQAFVYLKALREYHWNQLTEFVKYY"
BASE COUNT         1057 a          565 c          512 g          970 t
ORIGIN      
        1 ggataatgaa agaaaaagag tagcctcaga attacatgat gatttaggtg caggtctttc
       61 aaccatacga ttactgagcg aactagctct tcaaaagacc gagggaaaaa ctgaaataaa
      121 acgaatcagt gagttatcaa atgaacttgt tgactcaatg cgacaaataa tctggagcat
      181 gaatcctgaa agcagcagtt tatcagactt tattcattat ttaaagcgat atacgatgga
      241 aattatagaa ttaaacggaa aaatatttaa ttttaaatta aatcaagaga tttctgatta
      301 tacacttagt cctgagcaaa aaagaaattt atttctcata ataaaagaaa ctttgcacaa
      361 cagcattaag cattctaaat ctaacgaaat cattcttata tacgactata ttgaaaaaag
      421 gcatcaccta actatcaaag acaatggtat cggattttct cccgatgata ttaattacaa
      481 aaacgggaat ggtttaatta acattcaaaa aagagcaatg caaataggct gttctgtatt
      541 tgtaacttca gaaattacta aaggaactat cacaagaatc gaatttagtt aacaaaagtt
      601 atatctaatt tacccctcaa ataattaata ttgcaacatg attaaggtat gcattgttga
      661 ggacatacat gaaataagag acggatttaa gagtgtcttg gatctttcag gtgagttcaa
      721 atgttgtggc acatttggca atgctgaaga tgctattgat aaaattcctg agttaaatcc
      781 agaaattgtg ataatggaca tcaatctacc tggtataaat ggaatagaag caattaagta
      841 tttaataaaa aacaactgta acccattgtt tctaattttt accgtttatg atgatgatac
      901 aataatattt gatgcgttgc aagcaggtgc aagtgggtat ctactaaaaa aaacaacacc
      961 tgcacaattt ctggaagcag ttaaagagat gtacgatgga ggttcaccga tgagtccaat
     1021 tatagcacga aaagtgataa attcatttaa acctgggcaa aaaacaaatg ccattttaag
     1081 tgtacgtgaa tctgaaattc taacattact tgcaaaggga ctgttatata aagaaatatc
     1141 agaacaactt ttcattagta ccggaacagt aaaacagcat attcataaaa tttatgaaaa
     1201 gcttcatgtt cagaatagaa ctgaagcaat taataagttt ttgaacaaat gaaagtagaa
     1261 cgtatttcaa aacctgagcc gattagaact tgcgtataat tttctattaa attataaaaa
     1321 attaaatacc ccccaaagtc tgctgtaatt tatgtaagac ggcagccgtt aagtaaacaa
     1381 ctttacgcac gtttagtaaa aaaatcttac aagataactt tgagcacttc acgcaagtaa
     1441 tatatggcac taccccaaca gtgtctgctt acaagcatct aaaatctgaa atgctttttc
     1501 cctggaagat gattctgcat aggttctgag aacaggctct gttccgctgg cacggatcat
     1561 cagccattcg ctgttggtat tgtcgaagaa atatttaaat ccgtcaaggt catcaacacg
     1621 ttgtactttg taattgccaa actgtgcgta tttattctga aggcagtttg caagaatttt
     1681 ttgtttaact tcttctttga tatgaagatc gtttcgttcg aaagaaaatg gaccggttat
     1741 ttcatatact tctttaataa gttcattcag tgattttcct gacttagcca taaactccca
     1801 aattaccaat cccatccaaa taccatcacg ttccggaata tgtcctttaa tggcaatacc
     1861 accggactct tctccaccta acaatacatc ttctttaatc attatttcgc agatgtattt
     1921 gaaaccaatc ttaactacgt caatgggcag accgtaatgc ttacatagcg tctgcacttt
     1981 aggtgtggta gaaaatgccg tacaaacttt tccactcatt cctttgtact tatgcaggta
     2041 atgaataagc agcagaatga tgtgatgtga gtcaacaaaa tttcctttat catcataaag
     2101 tcctatacgg tcggcatcgc catcagtaac taatccgcaa ccaatatcac ctctcttctt
     2161 tatcaactcc gaaaactctg tcaggttttt atgaattggc tccggtgcct gaccatgaaa
     2221 tccgggatta tggtcgcaat gcaacaaggt aatttcagga aataattttt tcatcacatt
     2281 ctgcccagca ccatacatgg catcataagc ccagttaatt ccactctttt ttatagcgtc
     2341 aaggtcaaag ttttctttaa ccttgttgat atacattgtt tcaaggtcaa ctatctgtag
     2401 caatcctttc gcagtaaaat cttccaaaga caattttaaa gtgtctatgg ttgaagcatc
     2461 ttttatcata tcttcaattt ccgaaacctg tgcaggtaat aacggtccgc cataagaacc
     2521 tttcaattta aatccattgt acgatggtgg attgtgactt gctgttagaa taattcccaa
     2581 gtcggctttg taatgtaatg tacctagcga aaccatgggc gttgaaacaa aatccggtgc
     2641 caacatcact ttaattccgt ttgcacataa aacttttgca gtagtctcag caaagagatt
     2701 tcctgcaaaa cgacaatcgt tgcctacaac tactgaaggg ttaccaccct ttgcctttag
     2761 ccacagtgct gtagcttctg ctacacgtgt aacattttct actgtaaaat cttgtgcaat
     2821 gattgcacgc catccatcgg taccgaattt aatttttgtt gccataaatt tttataatgt
     2881 tcaataatat tttacaaatt ctgttagttg attccagtga tactcacgaa gtgcttttag
     2941 ataaacaaaa gcctgtctta gtgcaatatc ctgtgaatta atttttgtta ttgtgaaatt
     3001 aaatttttta tcaagcaagg caaggactaa tttctgacca ttgatttcta aatccatcat
     3061 agtcatttca gaaagtggaa tactatttaa tgcagaatca ttaa
//
LOCUS       DAORQT010000193         3081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6212_length_3081_cov_6.255453, whole genome shotgun sequence.
ACCESSION   DAORQT010000193 DAORQT010000000
VERSION     DAORQT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_3081_cov_6.255453"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK134_06795"
     CDS             complement(<1..245)
                     /locus_tag="PK134_06795"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRB38589.1"
                     /translation="MREKLASGDMIDPRYGYVFETQEVRPAEKRGFADFSAGVIGYSS
                     KVYGGFAVHHITEPDQAFIVEGSSPLPRKYTAHVGAL"
     assembly_gap    269..278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<279..672)
                     /locus_tag="PK134_06800"
     CDS             complement(<279..672)
                     /locus_tag="PK134_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRB38590.1"
                     /translation="MIKRIVLTMIISLELAICFGQDPQFSQFYANPIYLNPAFAGSVR
                     CPRLALNYRNQWPGLNKTFITLSASYDQHVEALSGGLGLIIMNDKAGNGTLNTTNFSG
                     IYSYNLNISRNFSIRAGLQATYVQEKLDW"
     gene            879..>3081
                     /gene="porU"
                     /locus_tag="PK134_06805"
     CDS             879..>3081
                     /gene="porU"
                     /locus_tag="PK134_06805"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRB38591.1"
                     /translation="MKRSFALLFLIGLTCVAVAQQDESLKRTYSVDWRSNVVLNGENR
                     LNFTNCFYKPDSKMPFFSTGLPLFDYTKVAEVSIENPVYEPLNINISDEVASEIVPET
                     KLSYFKGKPSLYVEFVPVRKNSVTGQTEKLVAFNLKTTAGQQSQKIGATSTQVYAANS
                     VLASGDWYKISVTEDGVYKITYSFLKDQLKIDLSSSSPQSFRLFGNGGGILPLLNSQF
                     RYDDLQENAVFVNDGGTAGKWDDQDYILFYGQSPVQWLFNSSQNRYYHQANYYSDSTF
                     YFITFNGGTGSPKRIQQSASLSVTPDFVVNSFDDYQYHELDKTNFVKSGREFYGESFD
                     INLNQTFSFTFPNILQQTVTIKTVFAGHSPGVTSSASARYQNITLFSGTYTTGSQYTD
                     DYAMERTTTSTFLPSAGDVVNIDFAFTPANTECIGFLNYIELLAKRALTFAGNGATDQ
                     MFFRSMEASGPGKTAQFNIASAPSSMTVWNVTDRINIRQQLHNNGSFITQVDSTQQFL
                     AFGGNNFLTPGVIGTVANQNLHSLPQADLIIVTHPLFEDQANRIADMHRTLDNMSVNV
                     VRTDLIYNEYSSGAQDIAGIRNFVKMFYDRGIGNGTEPKYLLLFGDGSYDNKYRRANN
                     TNFIPTFESENSYSFLSSFVADDFYGLMDDNEGLCSSNEKIDIGVGRIIAQNISEAKT
                     VTDKTIQYASLPQSSGCPGCDGSGGTLGDWRNVVTFIADDEDGPIHVPPAES"
BASE COUNT          986 a          552 c          590 g          943 t
ORIGIN      
        1 aataatgccc ctacgtgtgc agtatatttt cttggtaacg gacttgagcc ttcaacaata
       61 aatgcctgat caggttctgt aatatgatga acagcaaaac cgccatatac ttttgaactg
      121 taacctataa cgcctgctga aaaatcagca aaaccacgct tttcggcagg acgtacttcc
      181 tgcgtttcga acacataacc atagcgggga tcaatcatat cgccagaagc taatttctcc
      241 cgcattgcct gaatatacgg tgcaatttnn nnnnnnnncc cagtctaatt tttcctgaac
      301 ataggtagct tgcagccctg cacgaataga gaaatttcgt gaaatgttta agttgtaaga
      361 atagatgccg gagaagttgg ttgtattcag tgttccatta cctgctttat cattcataat
      421 gattaagcct aatccaccgg agagtgcttc aacatgttgg tcataagagg cgctcaaagt
      481 gatgaaggtt ttatttaaac caggccactg attacgatag ttcaatgcca gtcgtggaca
      541 acgaaccgaa ccggcaaagg caggattcag ataaatcgga ttggcataaa actgcgaaaa
      601 ctgcgggtcc tggccaaaac aaatagctaa ctccaggctg ataatcatgg taagtacaat
      661 acgcttaatc atagttcatt tacttttcaa acttaatttt atacgtatag tactaaaata
      721 gttacaattc tgcacgaata tattggtggt taaaaattta atcggaacaa agttaggcat
      781 taattatttt aatatcaaac aaaataaaat tcaatattgc atgtttcata catgttgtat
      841 tgttcgagaa gcatattttt gaacccaatt accaaaacat gaaaaggtct tttgcactac
      901 tttttttaat aggattgact tgtgttgctg ttgcgcaaca agatgaatct ttaaaacgca
      961 cttattccgt tgattggcgc agtaatgttg tcctaaatgg cgaaaacagg cttaatttta
     1021 ccaactgttt ttataagccg gacagcaaga tgccattttt ctctaccgga cttccactat
     1081 ttgattatac taaagtggct gaagttagca ttgaaaatcc tgtttatgaa ccattaaata
     1141 ttaatatttc tgatgaagtt gcatcggaaa tagttccgga aacaaaactt tcttacttta
     1201 agggaaaacc atctttgtat gtagaatttg tgccggtaag aaaaaattca gttacggggc
     1261 aaacagaaaa attagttgca tttaatctta aaacgacagc cgggcaacag agtcaaaaaa
     1321 ttggtgccac ttcaactcag gtttacgctg ctaattcagt tcttgcttcg ggtgattggt
     1381 ataaaatttc agttacagaa gatggagttt ataaaataac atacagtttt ctgaaagacc
     1441 agttaaaaat tgatttatca tcttcctcac ctcagtcatt caggctcttt ggcaatggtg
     1501 gaggcatttt acctctatta aactctcagt ttagatatga tgacttgcag gaaaatgctg
     1561 tgtttgtaaa tgatggaggt acagcaggaa aatgggatga tcaggattat attttgtttt
     1621 atggacagtc acccgtgcaa tggcttttta attcctctca gaacagatat taccatcagg
     1681 caaattatta ttccgattcc acattttatt ttattacgtt taatggagga accggaagtc
     1741 ctaaaagaat tcagcaatcg gcatcattat cagtgacacc tgactttgtt gtaaacagtt
     1801 ttgatgatta ccaatatcat gaattggata aaacaaattt tgtcaaatct ggacgtgaat
     1861 tttatggcga gtcattcgac ataaacctta atcagacttt ttcattcaca tttccaaata
     1921 tactgcaaca aactgtgact ataaaaacag tatttgcagg tcattcacct ggtgttacaa
     1981 gttcggcttc ggcacgctat caaaatataa cattattttc gggtacatac acaaccggtt
     2041 cacaatatac agatgattat gcaatggaac gtacaacaac ttcaacgttc ttgccgtctg
     2101 ccggtgatgt agtgaatatt gattttgctt ttactcctgc aaatacagag tgtattggct
     2161 ttttgaacta tatagaatta ttagcaaagc gagcactaac atttgcaggt aatggtgcta
     2221 ccgatcagat gtttttccga agtatggaag cttccggccc cggtaaaact gcacagttta
     2281 atattgcatc ggcaccatca tctatgacgg tttggaatgt cactgacaga ataaatatca
     2341 gacaacaatt acataataat ggttcgttta ttacacaggt ggacagtaca cagcaatttt
     2401 tagcttttgg tgggaataat tttttaacgc ccggagtaat tggtacagtg gcaaatcaaa
     2461 atttacattc attgccacaa gccgatttaa tcattgtaac acatccatta tttgaagatc
     2521 aggcaaaccg catagcagat atgcacagaa cattggacaa catgtctgtg aatgttgtga
     2581 gaacagattt aatatacaac gaatactctt caggagcaca ggatattgca ggcatacgca
     2641 attttgtaaa aatgttttat gacagaggga taggcaatgg aacagaacca aaatatttac
     2701 ttttatttgg tgatggttct tatgacaaca aataccgcag agccaataac accaatttta
     2761 ttccaacctt tgagtctgaa aactcatatt catttttaag ctcattcgtt gctgacgatt
     2821 tttatggttt gatggatgac aatgaaggtt tgtgttcaag caatgaaaaa attgatattg
     2881 gtgtaggtcg aattattgca caaaacatca gcgaagctaa aacggttaca gataaaacca
     2941 ttcaatatgc atcattgcca cagagtagtg gttgtccggg ttgtgatggt agtggtggaa
     3001 cattaggtga ctggcgcaat gtggtaacgt ttattgcaga tgatgaggat ggacctatac
     3061 acgtaccacc tgcagaatcg t
//
LOCUS       DAORQT010000194         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6261_length_3067_cov_4.940571, whole genome shotgun sequence.
ACCESSION   DAORQT010000194 DAORQT010000000
VERSION     DAORQT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6261_length_3067_cov_4.940571"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PK134_06810"
     CDS             complement(<1..909)
                     /locus_tag="PK134_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38592.1"
                     /translation="MKALAGYFSLWLKKNTFLLWALAVIHLIFFLLALRNGNIAFSVD
                     STEYLNQAENIREHASWYAGSSSTPHDDFLESRRPPLYGFFIFLVHIVSSNNFFLLAL
                     QNLMSIAVLILTAFMFWLLTGKRIANQWLVILPLLLFPTQFIYANMIMADVLFQFLII
                     SGFLFFIQSVRRPDSFLYFCIFISLAILTKPVFYAFCLIVPVIALLFYTKGIYKLSNV
                     YISLIPLLTVATISTINYSKTGYFHYSSVNEKFISEYGAYLAVGDKGDISAQQKIDSL
                     LSEANKQPDFKSYCKYINKESLFLIKE"
     gene            complement(929..>3067)
                     /locus_tag="PK134_06815"
     CDS             complement(929..>3067)
                     /locus_tag="PK134_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38593.1"
                     /translation="RVSLDPSLYPTTFKWKESSFIYIPQKTGWSQAHFFQFKNDSYTN
                     IVYDKPQRKWEYENTQAELRVSAWDVPGQGITSDNVQLNNQLIATGGCNYRAIYAVVL
                     DSISLQNWSTLDFAGKFGNINQPPYYDSCQATVNGSQGFFAFATDSAYSVGGNTVYRN
                     LDSLANFINRIPTGNYVVLYSVTEHFMSKSWSGQFRFALSQQGINNVDTIPDTKHFIF
                     FFKKGFPGTAQTVVAATDSTKNITFTTMLGGNWYKGYMMSEIIGPAIQWNSLHWANNS
                     VEAINTDTLALDIVGVDTLNSEHVLYSAIQPSQIDFILNINPFIYPRIYLRAYMEDSV
                     YRTPPQLNRWQIYYQEVPEAVLNPVGSSFQSNSVSQGDTIHWKMPVENVSLSDMSDLL
                     VDYYLFDKDNIRRNITSTRFHPLLHGDTLNAAVKFSTAAYPGLNSLWMEVNPRNDQPE
                     QYHFNNYARIDFNVNRDVTNPILDVTFDGQHILNGDIVSAKPQILIKLTDENRYLALN
                     DTSKFKVYVKSPDGVESQIYFETSANSALSEKLLRWTPAQLPDNTFKIEYNPIFNVDG
                     IYELRAQARDESGNLSGSNDFRISFEVINKSSITNVFNYPNPFTTRTQFVFTLTGSEV
                     PTYFKIQIYNISGKVVKEITQQQLGNIHIGRNITEYAWDGKDEFGDQLAKGVYLYRVI
                     TQLDGDDIEHRNTTADKYFRKGWGKMYLLK"
BASE COUNT          992 a          596 c          528 g          951 t
ORIGIN      
        1 ctctttaatc agaaacaaac tttctttatt aatatactta caataacttt taaaatcagg
       61 ttgcttattg gcttcactaa gcaaagaatc aatcttttgc tgtgcactta tatctccttt
      121 atctcccact gccagatagg caccatattc agagataaac ttttcattta cgctgctgta
      181 atgaaaatac ccggtcttgg aatagttaat tgttgaaatt gtggctacag tcaataatgg
      241 aatcagtgat atataaacat tgctcaactt ataaatgccc tttgtataaa acaacagtgc
      301 tatgactgga actatcaaac aaaaggcata aaaaaccggc tttgttaaaa tagccaacga
      361 tataaaaata cagaaataca aaaatgaatc aggccttctt accgattgaa taaagaacaa
      421 aaatccgctg ataatcaaaa actgaaacaa gacatcagcc attatcatgt ttgcataaat
      481 aaattgtgtc ggaaacagca ataagggtaa tattaccaac cattgattgg caatccgctt
      541 tccggtcagc agccaaaaca taaaggctgt aagtataagc actgcaatgc tcattaaatt
      601 ctgcaatgcc agtaaaaaga aattatttga tgaaactata tgcactaaaa aaataaagaa
      661 gccatataac ggaggccttc ggctttctaa aaagtcatcg tgtggtgttg atgaactacc
      721 agcataccat gaagcatgtt cgcgtatgtt ttctgcctga tttaaatatt ctgtactgtc
      781 aacagaaaag gctatgttgc catttcgcaa agccagaaga aaaaatatga gatgaatcac
      841 agccaaagcc caaagaagaa atgtgttctt cttcaaccat aatgaaaaat aaccggcaag
      901 ggctttcata ggtctttaaa aggttgcctt actttaagag atacattttt ccccatccct
      961 ttctaaagta tttgtcagca gtggtattcc gatgctcaat atcatcacca tcaagttgtg
     1021 ttattactct gtaaagataa acaccttttg ccaactgatc accaaattcg tcttttccat
     1081 cccatgcata ttctgtaatg tttcgaccaa tgtgaatgtt tcccaattgc tgttgtgtta
     1141 tttctttcac cactttgcca ctgatattat agatctgaat tttaaaatag gtaggcactt
     1201 ccgatccagt gagtgtaaac acaaactgtg ttcgtgttgt aaatggattt ggatagttga
     1261 acacattggt aatgctactc ttattaatta cctcaaaaga aattcgaaaa tcgttgctgc
     1321 cgctaaggtt accactttca tctcttgcct gagcacgaag ttcgtaaatg ccgtcaacgt
     1381 tgaaaatcgg attgtactct atcttaaatg tattatcagg aagttgcgca ggtgtccaac
     1441 gtagtaattt ctctgacaat gctgaattcg ctgatgtctc aaaataaatc tgactctcta
     1501 caccatcagg actttttaca tacactttaa attttgaagt gtcatttaaa gccaaatacc
     1561 tgttttcatc tgttagttta atgagtattt gcggctttgc agaaacaatg tcgccattaa
     1621 gaatatgttg tccatcaaag gtaacatcga gaattggatt tgtcacatca cggtttacat
     1681 taaaatctat tcttgcataa ttattgaaat gatattgctc cggttgatca tttcgcggat
     1741 ttacctccat ccacaatgag tttaatcccg gataagctgc tgtgctgaac tttaccgcag
     1801 cattcaatgt gtcgccatgt aacaaaggat gaaagcgtgt agaagttata tttcgtctta
     1861 tattatcttt atcaaacaga tagtaatcca caagcagatc actcatatca gaaagactta
     1921 cattctctac aggcattttc caatgaatgg tatcgccttg tgaaacacta tttgattgaa
     1981 aagaagaacc tacaggattc aacacagctt ccggaacttc ctgataatag atttgccaac
     2041 gattgagttg cggtggtgta cgataaacag aatcttccat gtatgcacgc aaataaattc
     2101 gcggataaat aaacggatta atattcagaa taaaatctat ctgcgatggc tgaatagcgc
     2161 tgtacaaaac atgttctgaa tttaatgtat ctacaccaac aatatcaagt gccagcgtat
     2221 ctgtattgat agcttccaca ctgttgtttg cccaatgcaa actgttccat tgaatggcag
     2281 gaccaataat ctctgacatc atatagcctt tataccaatt tccaccgagc attgttgtga
     2341 atgtaatatt ctttgtactg tctgtagctg caacaactgt ttgtgccgta cccggaaatc
     2401 cttttttgaa aaagaaaata aaatgtttag tatcaggaat tgtatctacg ttattaattc
     2461 cctgttgcga taatgcaaag cgaaactgcc ctgaccatga cttgctcata aaatgctcag
     2521 tcacgctgta taataccaca tagtttcctg ttggaatacg attgataaaa ttcgcaaggc
     2581 tgtcgagatt gcgatataca gtgttgccac caacgctata tgcagagtct gttgcaaaag
     2641 caaaaaatcc ctgactgcca tttactgttg cctgacaaga atcgtaataa ggaggttgat
     2701 tgatgttacc aaattttcct gcaaaatcaa gtgtactcca gttttgtaaa gaaatagaat
     2761 caagtaccac tgcgtaaata gcacgataat tacagccacc tgttgcaatc aactgattat
     2821 tgagttgcac attgtcagag gtaattcctt gtccgggcac atcccatgca gatacacgca
     2881 actctgcttg tgtgttttca tattcccact tacgttgtgg tttatcataa acaatgttgg
     2941 tgtagctgtc atttttaaat tgaaaaaaat gtgcctgact ccaacctgtt ttctgtggaa
     3001 tataaataaa agaactttct ttccatttaa atgttgttgg ataaagtgat gggtcaaggc
     3061 ttacacg
//
LOCUS       DAORQT010000195         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6348_length_3038_cov_8.328864, whole genome shotgun sequence.
ACCESSION   DAORQT010000195 DAORQT010000000
VERSION     DAORQT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6348_length_3038_cov_8.328864"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3038)
                     /locus_tag="PK134_06820"
     CDS             complement(<1..>3038)
                     /locus_tag="PK134_06820"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38594.1"
                     /translation="DSSGANSWLWDYGDGSTATAQNVSHTYTTPGTYTVSLQTQSNGS
                     TCSQTISNFATYIVGGGIANFTYNQTLCPPFIGTFTDSSLNAVSWLWNFGDGQTSTQQ
                     NPVHTYNQTGSYNVSLTITSANGCTSTKVEQYAMNFLTLGAAPSAHTLDTIPPLDVQF
                     SANSVGATSWLWTFGDGDSSTLENPMHTYQSTGPFTISLTIANDSCSYTYSFPPTNFG
                     SGGTFYDENHDTTQFTMHYDGCAPLTISFNNPFLNVVGSYWDFGDGNTSSQQNPVHTY
                     NVPGIYDVMLIANLSNASVDTIYHSQAVKVAGTPVTFIVNTVNTCNGSNIQALPSNLN
                     LQSYNWDFGDNQTSSQVSPIHSYANTSNYIISLQAQDTSGCTVIHSKSLYANADANAI
                     LSNKNRACASDTITFSVQNTNYVNYTWDFGDGTSATGNPIAHTYSDSGSYSVQVVITD
                     INGCTQTVNASQQITIVKSVADFNINMLSSSCTMVNVQLTNTSTGSTSYTWDLGDGTM
                     SSLTDVNKYYYYGPNPPYTYDIKLTAYNNGCASTKTLAQAVTIPDFDVKFSYSRSQGC
                     LPITINVTDSSLNAVAWKWSWGDGDTTFTQATQHTFTSLPTDKIKLFAKDINGCEKNY
                     ELDNIQLPVAEFTQSDTIGCNPKTIQFTDSSKNAHQWLWKFGNGSTSITPNPSINYSS
                     TGNFIPSLIITDSFGCKDTVGGKIISITEPQADFSYTKSSGCVPVIVQFQNTSSGAAN
                     YLWDFGDGSTSTLLNPNHIYTTGGNYQVTLTVTDASGCSNTKTLSNALHIPGPLASFS
                     LSNNHGCQPLTISTNDSSQNALSHFWSFGDGDTSVATSPIHTFNTAGMYLITLIVKDA
                     NGCQSVYTHPDTIKVYNKPVAAFAISDSTICVGTSISLLNQSQSSSSYKWQFGDGTVS
                     NQSNTSHQYQIPGQFSITLVASNGHCSDTANSINAVTVIPFPIADFGGSPLIGCAPLS
                     SNMVNISSSLQNPAFIWDGGNGLTSSAFNPQFNYQNAGYYT"
BASE COUNT          910 a          512 c          641 g          975 t
ORIGIN      
        1 gtataatatc ctgcattttg ataattaaac tgcggattga atgctgatga agtgagtcca
       61 ttgccgccat cccatataaa tgcagggttc tgtagtgatg agctgatgtt gaccatgttt
      121 gaagaaagcg gtgcacaacc aatcaatggc gaacctccaa aatcggcaat ggggaaaggt
      181 ataacggtaa cggcattgat gctgttagct gtatccgaac aatgaccatt agaagcaact
      241 aatgtaatgc taaattgccc tggaatctga tattggtgac ttgtatttga ctgattcgat
      301 actgtaccat ctccaaattg ccatttgtaa ctacttgatg attgagattg gttgagtaaa
      361 gaaattgaag ttccaacaca aattgtagag tcgctgattg caaatgctgc aacaggtttg
      421 ttgtaaactt taattgtatc aggatgtgta taaacagatt gacatccatt ggcatccttt
      481 acaatcaatg ttattaagta cataccggca gtattgaacg tgtgaatggg tgaagttgcc
      541 acagaagtat ctccatctcc aaaactccaa aagtgactaa gtgcattttg agaggagtcg
      601 ttagtgctga tagttaaagg ttgacaacca tgattgtttg aaagagaaaa tgaagctaag
      661 ggtcctggta tgtgtaaagc attggacaat gtttttgtat tactgcaacc cgatgcatct
      721 gttactgtaa gtgtaacttg gtagtttccg cctgttgtgt agatatgatt tggattcaag
      781 agtgttgatg tactgccatc tccaaaatcc caaagatagt ttgcagcacc cgaagatgta
      841 ttttgaaatt gaacaatcac cggaacacaa cctgaagatt ttgtataact aaaatcagcc
      901 tgtggctctg tgatggagat tatttttcca ccaacagtat ctttacatcc aaaagaatct
      961 gtaattatta aagatggtat aaaattgccg gtactactgt aattaatact tggatttgga
     1021 gttattgatg ttgacccgtt accaaatttc cataaccatt gatgtgcatt tttacttgaa
     1081 tctgtaaatt gtatggtttt tggattacat cctattgtat ctgactgcgt aaattccgct
     1141 accggaagtt ggatgttatc aagctcataa tttttttcgc atccattaat atctttagca
     1201 aaaagcttta ttttatctgt tggtaaagag gtaaaggtat gttgtgtagc ctgagtaaaa
     1261 gtggtgtcac catcgcccca agaccatttc catgctacag catttaggct tgaatcagtt
     1321 acattaattg tgataggtaa gcaaccttga cttcttgaat aactgaattt aacatcaaaa
     1381 tctggtatgg taacagcctg agctaatgtt ttagtgcttg cacatccatt gttgtatgca
     1441 gttagcttaa tatcataagt gtagggtgga tttggtccat agtagtaata tttatttaca
     1501 tcggttaatg atgacatagt tccatctccc aaatcccatg tataagaagt gctgcctgta
     1561 gaggtatttg ttaactgtac attcaccatg gtgcatgatg aggaaagcat attgatgttg
     1621 aagtcagcca ctgattttac aatggttatt tgttgtgaag catttactgt ttgcgtacaa
     1681 ccgttaatat ccgttataac aacctgaaca gaataagatc ctgaatcaga gtatgtgtga
     1741 gctataggat ttcctgttgc cgaagtgcca tcaccaaagt cccaagtata gtttacatag
     1801 tttgtgtttt gaactgaaaa ggtaatagtg tctgatgcac atgctctgtt tttattggaa
     1861 agaatagcgt tggcatcagc attggcatat aaactcttgg aatgaattac agtacatcca
     1921 ctagtatctt gtgcctgtaa agaaataatg taattgcttg tattggcata gctatgtatt
     1981 ggtgaaacct gtgagcttgt ttgattatca ccaaaatccc aattatagct ttgcagattc
     2041 agatttgatg gtaaagcctg aatgtttgag ccattacatg tattaactgt attaacaata
     2101 aatgttaccg gtgttcctgc cacttttaca gcctgtgaat gataaatagt atcaacactt
     2161 gcattagata aatttgcaat gagcattaca tcgtaaatgc cgggtacgtt gtatgtgtgt
     2221 accggatttt gttgagacga agtattgcca tcaccaaaat cccaatacga gccaacaaca
     2281 tttaaaaatg ggttattgaa acttatggtc aatggtgcgc aaccatcata atgcatggtg
     2341 aattgtgtcg tatcatggtt ttcatcataa aatgttcctc cacttccaaa gttagtagga
     2401 gggaaactgt aggtataact acaactgtcg ttggcaatcg tcagacttat tgtgaaaggg
     2461 cctgttgatt gataggtgtg cataggattt tcaagtgttg aagaatctcc atcgccaaaa
     2521 gtccatagcc atgatgttgc acctactgag ttggccgaaa actgaacatc aagaggagga
     2581 atggtatcta atgtatgggc agaaggtgca gcacctaagg ttaaaaagtt cattgcatat
     2641 tgttcaacct ttgttgatgt acatccattt gcagaagtga tggttaaaga aacattgtaa
     2701 ctaccggttt ggttgtaggt gtgaacagga ttttgttgtg ttgatgtttg tccgtcacca
     2761 aaattccata accatgaaac agcatttaaa ctgctgtctg taaatgttcc gataaatggc
     2821 ggacaaagtg tttggttata tgtaaaatta gctataccac caccaacaat atacgtggca
     2881 aaattgctga tggtttgaga acatgttgag ccattgcttt gcgtttgcag acttactgta
     2941 tatgttcccg gtgtagtata tgtatgagaa acattttgtg ctgtggcagt actgccatca
     3001 ccataatccc aaagccatga gtttgcacct gatgagtc
//
LOCUS       DAORQT010000196         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6473_length_2995_cov_8.803401, whole genome shotgun sequence.
ACCESSION   DAORQT010000196 DAORQT010000000
VERSION     DAORQT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_2995_cov_8.803401"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1087
                     /locus_tag="PK134_06825"
     CDS             113..1087
                     /locus_tag="PK134_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB38595.1"
                     /translation="MPKVTIDGHTIEVPEGTTILEAARKIGGDIVPPTMCYYSKLKTS
                     GGYCRTCLVKVSKGSEKDPRPMPKPVASCRQTVMDGMEVQNITSPEILDARAGVVEFL
                     LINHPLDCPVCDQAGECNLQDLSYEHGHEVSRLDFRKRTFPMIDIGDKIKLHMNRCIM
                     CYRCVKVAEQLCPERVHGVLNRGWDSEISTYIEQAVDNEWSGNMIDVCPVGALTDKTF
                     RFKSRVWFTKPVDAHRDCPTCSGKVVLWYQGEEVLRVTGRKDQWGEVDEFICNECRFD
                     KKKTSDWVIEGPRHIDRHSVISQNHYEELKKLKQDLSRQKKLGEKAHN"
     gene            1097..2137
                     /gene="nuoH"
                     /locus_tag="PK134_06830"
     CDS             1097..2137
                     /gene="nuoH"
                     /locus_tag="PK134_06830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRB38596.1"
                     /translation="MEVSVLIFKTVFVIAVFLVSLLIAMYSTYAERKVAAFLQDRVGP
                     NRAGPFGILQPLADGVKMFMKEEIIPSHADKALFILGPSLMMLTACMTGVVIPWGDTL
                     IINGKEYFMHITDVNIGVLYIFGVVSIGVYGIMIGGWASNNKYSLLGSVRASSQMISY
                     ELAMGMSIIALIMVTGTLSVREIVVQQQGFHWNVFYQPLGFLIFLICAFAECNRTPFD
                     LPECETELVGGYHTEYSSMKLGFYLFAEYINMFISSAVISALYFGGFNMPFISELGLS
                     HNALTLLGVLFYFIKIFFFIFFFMWVRWTIPRFRYDQLMNLGWKMLIPLSILNVLLTG
                     VFLLWKNGLFHF"
     gene            2152..2721
                     /locus_tag="PK134_06835"
     CDS             2152..2721
                     /locus_tag="PK134_06835"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB38597.1"
                     /translation="MTKELLIVGGPNGSGKTTFAKSFLQEYKYEFLNADEIARELSND
                     GSRAGNISSGKEYFKRIEKLKAKNKNIILESTLSGLFLRKLIREFKKKDYSIKIVFIV
                     LNAPEQCIGRIKHRVETGGHFVPDDDVRRRFTRGKNNFWKIYKDLVEDWKLFNNTESG
                     FEIIAAGEKNKYDIVNQSLFDLFIKDIEK"
     gene            2718..2933
                     /locus_tag="PK134_06840"
     CDS             2718..2933
                     /locus_tag="PK134_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38598.1"
                     /translation="MKSSKFKNYKNSNLFKEALEFAAIGNRGVQLAMIDSKKRKIPTV
                     FGIMDMVFYRLPDGSITTKSPFKKRKK"
BASE COUNT         1014 a          449 c          621 g          911 t
ORIGIN      
        1 ctgttgcatc atcaatcaga cacttcagaa gcgagtttga agcttatatc aacaatcacg
       61 gaatgaaaga cgtgaaacat tattacagac taaataattt acaagaagca taatgccaaa
      121 agttactata gatggtcaca ccatcgaagt tcctgaagga acaaccatac tggaagcagc
      181 ccgaaaaata ggtggtgata ttgtgccacc aacaatgtgc tattattcca aattgaaaac
      241 gtcaggtggt tattgccgca cttgtttggt taaagtaagt aaaggctctg aaaaagatcc
      301 gcgaccaatg ccaaaacctg ttgcatcgtg cagacagact gtaatggatg gtatggaagt
      361 acaaaacatt acatcgcctg aaattttaga tgcacgcgca ggagtagttg agtttttatt
      421 aatcaatcac ccacttgatt gcccggtttg tgatcaggca ggagaatgta atcttcagga
      481 tttaagctac gagcatggcc atgaagtgtc acgtcttgat ttcaggaaaa gaacttttcc
      541 gatgatagat attggcgata aaattaaact gcacatgaac cgatgtatca tgtgttatcg
      601 ctgtgtgaaa gttgctgagc aattatgtcc tgaaagagtt catggtgttt taaacagagg
      661 atgggattca gaaatatcta cctacattga acaagctgtt gacaatgagt ggagcggaaa
      721 catgattgat gtttgtcctg ttggtgcatt aaccgataaa acattccgtt tcaaaagtcg
      781 tgtgtggttt accaaacctg tagatgcaca tcgcgactgc ccaacatgtt caggcaaagt
      841 tgtattatgg tatcagggag aagaagtgct tcgtgttaca ggcagaaaag atcaatgggg
      901 agaagtggat gaatttattt gtaatgaatg tcgcttcgat aagaaaaaaa caagcgactg
      961 ggttattgaa gggccacgtc acattgaccg ccattcagta atctcacaaa atcattacga
     1021 agagttgaaa aaactgaagc aggatttatc acgtcagaaa aaattaggag aaaaagcaca
     1081 taattaaatt ttagaaatgg aagtttcggt attaatattc aaaacagttt ttgtaattgc
     1141 tgtctttctg gtttcattgc tgatagccat gtattcgacc tatgcagaaa gaaaggtggc
     1201 ggcattttta caagacagag ttggtcctaa ccgtgcaggg ccattcggta ttcttcagcc
     1261 attagctgat ggtgtaaaaa tgtttatgaa agaagaaatt attccatcac atgccgataa
     1321 agcattgttt atattaggtc caagcctgat gatgcttaca gcttgtatga caggtgtcgt
     1381 aattccatgg ggcgatactt taattatcaa tggaaaagaa tactttatgc atattaccga
     1441 tgtaaacatt ggtgtactat atattttcgg tgtggtttct attggtgttt acggtataat
     1501 gattggcgga tgggcatcaa acaacaaata ctctctgctt ggttcggttc gtgcatcgtc
     1561 acaaatgatc agttatgaac ttgcaatggg aatgtcaatc attgcattga taatggttac
     1621 aggcactttg agtgttcgtg aaattgttgt tcagcagcag ggctttcact ggaatgtatt
     1681 ttatcagcca ttaggatttt taatttttct gatttgtgct tttgcagaat gtaaccgtac
     1741 accgtttgat ttaccggaat gcgaaacaga gttggttgga ggctatcaca cagaatactc
     1801 ttcgatgaaa cttggcttct atttatttgc cgagtacatc aatatgttta tttcatctgc
     1861 tgttatttct gcactttatt ttggcggatt caacatgcca tttatcagtg aacttggatt
     1921 atcgcataat gcattgacat tgctaggtgt attattctat tttataaaaa tattcttctt
     1981 tatctttttc tttatgtggg taaggtggac aataccccgc ttccgatacg atcagttgat
     2041 gaaccttggt tggaaaatgc tcattccatt atctattcta aacgttttgc tgaccggtgt
     2101 gttcttgtta tggaaaaacg gactgtttca tttttaagca tcagatgaaa agtgacaaaa
     2161 gaattattaa tagtaggagg accaaatgga agtggtaaaa caacttttgc aaaatcgttc
     2221 ttacaggaat ataaatacga atttttaaat gcagatgaaa ttgcaagaga attatcaaat
     2281 gatggtagca gggcaggaaa tatttcatca ggaaaagaat actttaaacg gatagaaaaa
     2341 ttaaaagcaa aaaataaaaa catcattctt gaatcaactt tgtcaggttt gtttctgaga
     2401 aaattgatca gagagtttaa aaagaaagat tacagtataa aaattgtttt tatagtatta
     2461 aatgctccgg aacaatgtat aggaaggata aaacatagag ttgaaacagg aggacatttt
     2521 gtaccagatg atgatgttag gagaaggttt acaagaggga aaaacaactt ctggaaaatt
     2581 tataaagatt tggttgaaga ctggaaatta tttaacaaca cagaatcagg atttgagata
     2641 atagcagccg gagagaagaa taaatatgac attgtcaatc aatcattatt tgatttattt
     2701 ataaaagata ttgaaaaatg aaaagtagta agtttaagaa ttataaaaac agcaatttgt
     2761 ttaaagaagc attggaattt gcagccatcg gtaaccgggg tgtgcaatta gcaatgattg
     2821 attctaaaaa aagaaaaata ccaactgttt tcggaatcat ggatatggtt ttttacagac
     2881 tgccagatgg ttctataact acaaaatcac cattcaaaaa aagaaaaaag taaacaacat
     2941 tcattacatt tgtaaacggt tattattata attaatcatg ttaaccaata gaagt
//
LOCUS       DAORQT010000197         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6491_length_2990_cov_8.196252, whole genome shotgun sequence.
ACCESSION   DAORQT010000197 DAORQT010000000
VERSION     DAORQT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_2990_cov_8.196252"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PK134_06845"
     CDS             <1..1082
                     /locus_tag="PK134_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRB38599.1"
                     /translation="LRLIKMIIAPLVFATLVVGVAKVGDFKSVGRIGLKTILYFQFAT
                     ILALLLGLLLVNFFEPGKIMNLQIPAQSASTGVSAKGLNAKDFITHVIPKSIVEAMAN
                     NDILPIVVFALFFGLAAAAVGEKAKMVVNAMDAVSHIMFKVTNFVMKFAPYGVFGAVA
                     AVVAKQGLSVISGYLYLIATFYGGLLFFGLIVLGSICYFLKIPFIKLLQHIREPIMLA
                     FSTASSESAFPKTIEALEKYGCSNRIISFVLPLGYSFNLDGSIMYMAFATQFIAQAYG
                     MDLNIQQQIMMLLMLLVTSKGMAGVPRASMVVIAGMLESFNIPEAGLLLILGVDQILD
                     MGRSATNVVGNAVATAVVSKWEKEL"
     gene            1185..1673
                     /locus_tag="PK134_06850"
     CDS             1185..1673
                     /locus_tag="PK134_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRB38600.1"
                     /translation="MKKLFFAALILCATTAVNAQDDTKKAPAANPNAPDISFESETHD
                     FGTIPYSGNGTYEFKFTNTGKEPLVISNAKGSCGCTVPSWPKEPILKGHSGVISVHYD
                     TKRPGPFTKTVTVSSNGKTAEKVLTIKGNVETKEQTESGTPVKKDNTMTPKETTTSPF
                     GN"
     gene            1724..2173
                     /locus_tag="PK134_06855"
     CDS             1724..2173
                     /locus_tag="PK134_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRB38601.1"
                     /translation="MKKTILLSALAIGMATATFAQEKAAAVQVAPAENKNQADFKFET
                     LEYNYGTIKQGDKVNYTFNFVNTGKEPLIITNAQGSCGCTVPEWPKEPIKKGEAGKIH
                     VTFNSAGKMGLQDKTITITSNAKTSPVVLHIKGNIEAAAATQEAPKN"
     gene            2262..>2990
                     /locus_tag="PK134_06860"
     CDS             2262..>2990
                     /locus_tag="PK134_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB38602.1"
                     /translation="MPSISEKGKHMPPSPIRKLVPFADKAKAEGKKIYHLNIGQPDIE
                     SPKVMLDAIRSIDLKVIEYSHSAGIESYRRKLISYYKKYNININFEDILITTGGSEAI
                     EIAMMTCLNEGDEIIIPEPFYANYNGFSTQAGVTVVPVFSSIRNGFALPSHDEFEKVI
                     TPRTKAIMICNPSNPTGHLYSKEELQSLRDLALKHDLYLFADEVYREFCYDNNSYFSI
                     MHLEGLDENIILLDSISKRYSACGA"
BASE COUNT          972 a          500 c          587 g          931 t
ORIGIN      
        1 ttcttcgctt gattaaaatg attatcgctc cattagtttt tgcaacgctg gtagttggtg
       61 ttgctaaagt gggcgatttt aagagcgtag gacgcattgg cctaaagaca atattgtatt
      121 ttcagtttgc aactattctt gcgcttcttc tcggattact cttggtaaac ttttttgaac
      181 ccggtaagat tatgaatcta cagatacctg cacagtcagc atctacagga gtttcagcaa
      241 aaggtctcaa tgctaaagat tttattaccc atgttattcc aaaaagtatt gtagaggcaa
      301 tggccaataa tgatatttta ccaattgtag tatttgcttt gttttttgga ttagcagcag
      361 cggctgttgg cgagaaagcc aaaatggttg ttaatgcaat ggatgcagtt tcgcatatta
      421 tgtttaaggt tacaaacttt gtaatgaagt ttgcacctta tggtgtgttt ggtgcagtag
      481 ctgctgttgt tgcaaaacag ggactcagtg ttatttcagg ttatctctat ttaattgcaa
      541 ccttctatgg aggtctgctt ttctttgggt tgattgtatt ggggtcgatt tgttattttt
      601 tgaaaatacc atttataaaa cttttacaac atatacgcga acctatcatg ttggcattct
      661 cgacagcaag ttccgaatcg gcatttccta aaactattga ggcattggaa aaatatggtt
      721 gcagcaatcg cataattagt tttgtattac ccttaggcta ttctttcaac cttgatggaa
      781 gtataatgta catggcattt gctacgcagt ttattgctca ggcttatggc atggatttga
      841 atattcagca gcagattatg atgttgctga tgctacttgt taccagcaaa ggaatggcag
      901 gtgtgccaag agcgagtatg gttgttattg ccggaatgct cgagtcattt aatattcctg
      961 aagcaggatt gttgttgata cttggagttg atcagatatt ggatatgggt cggtcggcaa
     1021 ccaatgttgt aggcaatgca gtggcaacgg cagtagtgtc taaatgggag aaggagttat
     1081 aacgttcata cttttttcac agcctaagtc taactttggc gcattgtttg tcgttaaaga
     1141 gtaaaataat ttaactttac accacaaata aattaaatca aaatatgaag aaattatttt
     1201 ttgcagcact gattttatgt gcaacaactg cagtaaatgc acaggatgat acaaaaaaag
     1261 cacctgctgc taatcctaat gcacctgata ttagctttga aagcgaaacg catgatttcg
     1321 gtacaatacc ttatagtggt aatggtacct atgagtttaa atttacaaat acaggtaaag
     1381 aaccattagt aatttctaat gcaaaaggaa gttgcggatg tacagttcca tcgtggccaa
     1441 aagaacctat tcttaaaggt cattccggtg tcatcagtgt tcattatgat actaagcgtc
     1501 cggggccatt tactaagact gtaacagttt cttctaatgg aaaaacagca gaaaaagtgt
     1561 tgactattaa aggaaatgtt gaaacaaagg agcaaacaga aagtggaact cctgttaaaa
     1621 aagataatac aatgactcca aaagagacca ccacaagtcc atttggtaat taatcacata
     1681 aaataaatat taaacacaca aatcaacaat caaatttcaa aaaatgaaaa aaacaattct
     1741 tctttcagca ttagctatcg gtatggcaac tgctacattc gcacaagaaa aagcagcagc
     1801 agttcaggtt gctcctgctg aaaacaaaaa tcaggctgac tttaagtttg aaactttgga
     1861 gtacaattat ggtactatta aacaaggtga caaggtgaat tacactttta actttgtgaa
     1921 cacaggaaaa gagcctttaa ttattactaa tgcacaagga tcttgtggct gtacagtacc
     1981 tgagtggcca aaagagccaa ttaaaaaggg cgaagccgga aaaatccatg taactttcaa
     2041 ttcagcaggt aagatgggtc ttcaggataa aactattaca attacttcta atgcaaaaac
     2101 aagccctgtt gttttacaca ttaaaggaaa tattgaggct gcagctgcta cacaagaagc
     2161 accaaagaat taactaaggt aattagatta attataattt tgcagggacg attgaattca
     2221 atcgtccctt attttttgta tgtaattaat ataaaaactg catgccatct atttcagaaa
     2281 aaggcaaaca catgcctcct tcaccaatca gaaaattggt gccttttgcc gataaagcca
     2341 aagcagaagg aaaaaaaata tatcacctca atattggtca gcctgatatt gaatccccaa
     2401 aagtaatgct cgatgcaatc aggagtatag accttaaagt tatagagtac agccattctg
     2461 ctgggataga atcatatcga agaaagttga tttcgtatta caagaagtat aatatcaata
     2521 ttaattttga agatatttta attacaaccg gtggatcaga ggcaattgaa attgccatga
     2581 tgacctgttt gaatgaaggt gatgagatta ttattcctga accgttttat gccaattata
     2641 atggtttttc cacgcaagcc ggagttactg tcgtacctgt tttttcatct attagaaacg
     2701 gatttgcact tccatcacat gatgagttcg aaaaagttat tacacctcga actaaagcca
     2761 tcatgatttg taaccctagt aatcctactg gccaccttta ttcaaaagaa gagctgcaaa
     2821 gtttgcgcga ccttgctttg aaacatgatt tgtatctttt tgctgatgaa gtttatagag
     2881 aattttgtta tgataacaac agctattttt caataatgca tcttgaagga ttggacgaaa
     2941 atattatttt gttagactcc attagtaagc gatatagtgc ctgcggtgca
//
LOCUS       DAORQT010000198         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6678_length_2941_cov_9.009356, whole genome shotgun sequence.
ACCESSION   DAORQT010000198 DAORQT010000000
VERSION     DAORQT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6678_length_2941_cov_9.009356"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /gene="alr"
                     /locus_tag="PK134_06865"
     CDS             <1..1645
                     /gene="alr"
                     /locus_tag="PK134_06865"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRB38603.1"
                     /translation="ASIENAIHCWAMLLFLEIEQETIAERMMLLSPVAMRLEMKQGIN
                     NCSIINDSYNSDLGSLQVALDFLNRFQQQNNTGAINSGLRKTVILSDILQSGRNEDQL
                     YGEVAEMIHSKKVDRLIGIGPAISRHTDLFKTEKVFFSTTEKFIEQYSPGSFINEVIL
                     LKGARVYGFEQISRLLQQKGHETVLEINLTSMIHNYNFYKAKLKPETKIMCMVKAFAY
                     GSGSYEVANTLQFHRVDYLAVAYADEGIELRKNGITVPIMVMNPQEQSFDKMIEYHLE
                     PEIYSFRIMSKFSESVKRIRTYNQSPFPIHLKMDTGMHRLGFEEDDLNELVVRLKNSK
                     HLKVESIFTHLAGTDEAALDFFTQEQFQRFKDMSDAIKKHLQHNVLEHVLNSSGISRF
                     PEMQLSMVRLGIGLHGIGTPSEQRQLLNVASLKSTISQIRSVKKGESVGYNRKAILDS
                     DKIIATIGIGYADGINRKMGNGKVSFLVNGKLAPTVGNICMDMCMLDITNCQAREGDE
                     VIIFGNGLPVAELAAKTETIPYEILSVISQRVKRIYFQE"
     gene            complement(1646..>2941)
                     /locus_tag="PK134_06870"
     CDS             complement(1646..>2941)
                     /locus_tag="PK134_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRB38604.1"
                     /translation="FHLHLCVFMLRRVLIFLLIVFLIDLYVFQGIKVLIKDLTPSMSR
                     LISGIYWFFTVFTFSVFIAGLITDWHTWPKAVRVYLFAAVMIILITKLFVVLFLLIDD
                     LTRLVRWGFSGLYQRFYDSPQIVNPEEKIVVSRSQFLVRMAFVISSIPFISLLYGMGD
                     GKYNYQVKRRKIKFPKLPSTFEGFRIVQISDIHTGSFFDTKHIEKAVSMINTLKGDVI
                     FFTGDLVNDRSDEAVPFIDTLSKLNAKHGVFSILGNHDYGDYVQWPSREAKVKNLEQL
                     KNIHKEMGWKLMLDEHAFISEGNDKLGLIGVQNWSTHMRFPKYGSLPKAVQGMEFAPV
                     NILLTHDPSHWHGEVTENYPQIDLTLAGHTHGFQFGVEIPALKIKWSPVQYVYKEWAD
                     LYDDVNGQHLYVNRGLGFLGYPGRVGILPEITVIELSKG"
BASE COUNT         1014 a          555 c          515 g          857 t
ORIGIN      
        1 tgcttctatt gaaaatgcta ttcattgctg ggcaatgttg ctctttttgg aaattgaaca
       61 agaaacgatt gctgaaagga tgatgttact gagtcctgtt gccatgcgat tagagatgaa
      121 acagggtatc aataattgtt caataattaa tgacagctat aattctgatt taggttcgtt
      181 gcaggttgcc ttagattttt taaatcgctt tcagcagcaa aacaatacag gagcaattaa
      241 ttcaggctta cgcaaaaccg ttatcctttc agacattctt cagagtgggc gaaatgaaga
      301 tcaactttat ggtgaagtgg cggaaatgat tcactctaaa aaagttgaca gacttattgg
      361 aattggccct gcaatatcac gtcatacaga tttatttaaa acagaaaaag tttttttttc
      421 tacaacagaa aaattcattg aacagtattc tccaggctca tttattaatg aagttatatt
      481 attaaaaggc gcaagggttt atggatttga acagatttca agattgttac agcaaaaagg
      541 tcatgaaaca gttttggaaa taaatctgac ttcaatgatt cataattata acttctacaa
      601 agcaaagctc aaaccggaaa caaaaataat gtgtatggtt aaagcttttg catacggtag
      661 tggcagttac gaagttgcca atacactgca atttcacaga gtggactatc tggctgttgc
      721 ctatgctgat gaaggtattg aactcagaaa aaatggtatt actgttccaa ttatggttat
      781 gaatccgcag gaacagtctt tcgataaaat gattgaatat catttggagc ctgagatata
      841 tagtttcaga atcatgagca agtttagtga atctgttaaa cgaatccgga cttacaatca
      901 aagcccattt cctattcatc taaaaatgga tacaggcatg cacaggcttg gctttgaaga
      961 agacgatttg aatgaactcg ttgtacgttt aaaaaacagt aaacacctta aggtagaatc
     1021 tatttttaca catctggcag gaactgatga agcagcactc gactttttta cacaagaaca
     1081 gtttcagcga ttcaaagaca tgtctgatgc tataaaaaaa caccttcagc acaatgtgtt
     1141 ggagcatgtt ctgaattcat caggcatcag tcgctttcct gaaatgcaac tgagcatggt
     1201 gcgtcttggc attggccttc atggcatagg cacaccttct gagcaacgtc aattattaaa
     1261 tgttgcttca ttgaagtcaa ccatttcaca aataagaagt gtaaaaaaag gagaaagtgt
     1321 tggttacaac agaaaggcaa tattggatag cgataaaata atcgcaacca taggcattgg
     1381 ttatgccgat ggcataaacc ggaaaatggg aaatggaaag gttagctttt tagtgaatgg
     1441 caaacttgcc ccaacagttg gcaacatctg catggacatg tgtatgcttg acattacaaa
     1501 ctgtcaggca cgcgaaggtg atgaagtaat tatttttggc aacggtttgc ccgttgccga
     1561 acttgctgca aagacagaaa ccattcctta cgaaatttta tcagtgattt cgcaacgtgt
     1621 caaacgaata tattttcagg aataattaac ctttcgataa ttctattaca gtaatttccg
     1681 gaaggatacc taccctaccc ggataaccaa gaaaaccaag tccgcgattt acatacaaat
     1741 gctgaccatt tacatcatca tacaaatcag cccactcttt ataaacgtac tgaacagggc
     1801 tccatttaat ttttaatgca ggaatctcaa caccaaactg aaatccatga gtatgccctg
     1861 ctaaagttaa atcaatctgt ggataatttt cagttacttc tccatgccaa tgcgaaggat
     1921 cgtgcgtaag taaaatatta acgggagcaa attccattcc ctgaactgct tttggcaggc
     1981 taccatactt tggaaaacgc atgtgtgtac tccaattctg tactccaatt aatcccaact
     2041 tatcattgcc ttcagaaata aatgcatgtt catcaagcat cagtttccaa cccatttcct
     2101 tatggatatt tttcagttgc tccaaatttt taactttagc ttctctgctt ggccattgca
     2161 catagtcgcc ataatcgtga ttacctaaaa tagaaaaaac accatgtttt gcattgagct
     2221 ttgacaatgt atcaataaaa ggcactgcct catcagagcg gtcattcacc aagtcaccgg
     2281 taaaaaaaat cacatcccct tttaatgtgt taatcattga aacagctttt tcaatatgtt
     2341 ttgtatcgaa aaagctgccg gtatgaatat cagaaatctg cacaattcta aagccttcaa
     2401 atgttgaagg taattttgga aacttaattt tcctccgttt gacctgataa ttatacttac
     2461 catctcccat gccatacaag agagaaataa atggaattga tgaaattaca aatgccattc
     2521 gcactaaaaa ctgcgatctc gatacaacaa tcttctcttc aggatttaca atttgtggag
     2581 aatcataaaa tcgctgatat aaacctgaaa atccccatct gactaatctg gtaagatcgt
     2641 caatcaacag aaacaaaaca acaaagagtt ttgttattaa aattatcata acagcagcaa
     2701 agagataaac tctcactgcc tttggccatg tatgccaatc tgtaatcagt ccggctataa
     2761 atactgaaaa tgtaaacact gtaaaaaacc aataaatacc tgaaatcaat cttgacattg
     2821 agggagtcaa atccttaatc aataccttta taccctgaaa aacataaagg tctatcagaa
     2881 aaacaattaa taaaaaaatc aatactcttc gcaacataaa aacgcacaaa tgtaagtgaa
     2941 a
//
LOCUS       DAORQT010000199         2928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6721_length_2928_cov_4.844065, whole genome shotgun sequence.
ACCESSION   DAORQT010000199 DAORQT010000000
VERSION     DAORQT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_2928_cov_4.844065"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK134_06875"
     CDS             complement(<1..262)
                     /locus_tag="PK134_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRB38605.1"
                     /translation="MNILIIAATKQEISLFQIEKSEEIHIYKSHNVEILISGPGILAT
                     AFHLTRKLKEKQYDLIINAGIAGSLDENIIPGDVVQVKSEQIA"
     gene            334..753
                     /locus_tag="PK134_06880"
     CDS             334..753
                     /locus_tag="PK134_06880"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRB38606.1"
                     /translation="MIYITRKERFNAAHKLYKPEWSEEKNKEVFGKCSNPNWHGHNYE
                     LFITVKGMPNPETGFVIDLKVLSQIVKEKVIDPLDHKNINLDVKFMQGIMASTEMLAV
                     QIWNQLYDAINSQGAMLHAVKLYETENNFVEYFGNEK"
     gene            750..1391
                     /gene="folE"
                     /locus_tag="PK134_06885"
     CDS             750..1391
                     /gene="folE"
                     /locus_tag="PK134_06885"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRB38607.1"
                     /translation="MNKDPHTIIDDDDDPEGYRKIDHYNHDSLNKLSQSYLEVLQNLG
                     ENTSREGILKTPERVAKAMQFLTQGYHLNAKEILESARFREDYKQMVIVKDIEVYSLC
                     EHHMLPFFGKAHVAYIPNNYIVGLSKIPRVVDVFARRLQVQERLTVEIRDAIQETLSP
                     LGVAVVIEARHLCMQMRGIQKQNSVTTTSAFTGEFENDRTRSEFLRLIADKHI"
     gene            1397..1882
                     /locus_tag="PK134_06890"
     CDS             1397..1882
                     /locus_tag="PK134_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38608.1"
                     /translation="MTQKKFLVFVVEDDRHLQKMIVDYLTEHFPDIEINLYKTGEDAL
                     IDLKLGPDLIILDYFLSLYDKDAMDGLTVLSHIRQANKTVRVVMFTGQEDPDVAASAI
                     KMGAFDYIVKSPESFNKLSEIITQLKKQIVNETPPFDKRLLIVLLIGIIMLSALFIFR
                     H"
     gene            1917..2687
                     /locus_tag="PK134_06895"
     CDS             1917..2687
                     /locus_tag="PK134_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB38609.1"
                     /translation="MKLKIIKDNLLKAYLGQVPKGLQKAFDNLQDAELSTPNFSFYTS
                     VATVFSSKIEGESIELDSYIKHRRDGISFQPDYTKKIDDLYNAYLFAQENKLNEQNVK
                     QAHAILSKHIVTNEWQGRYWQQNMYVTSEDGRIEYVAALPQIVEDEILKLFEDIDLLI
                     KTNLSLGKVFYYAAMIHLVFVKIHPWNDGNGRSARLIEKWFLSEKLGAKAWFMQSEKM
                     YYSRHSEYYNCLKQLGLEYENLNYQKALPFVLMLPDCV"
BASE COUNT          983 a          480 c          566 g          899 t
ORIGIN      
        1 ctgcaatctg ctcagattta acctgaacaa catctccggg aataatattt tcatctaaac
       61 ttccggcaat accggcatta ataatcaggt catattgctt ttctttcagt tttcttgtca
      121 gatgaaaggc agttgccaga attcctggcc ctgatatcaa tatttctaca ttatgactct
      181 tataaatatg aatttcttca gatttttcta tctgaaatag acttatttct tgttttgttg
      241 ctgcaattat gagtatattc acggggtgta aaattaatgt tttaatagtg gcattggatg
      301 agttttttac ctttgcgaaa attcagcaac tgaatgattt atattacaag aaaagagcgg
      361 tttaatgcag cacataaatt gtataaaccg gagtggagcg aagaaaaaaa caaagaagtg
      421 tttggcaaat gctccaatcc taactggcat gggcataact atgagttgtt tataactgta
      481 aaaggtatgc ccaatcccga aacaggcttt gttattgatt taaaagtgtt gagccaaatt
      541 gttaaggaaa aagtaataga tccactcgac cataaaaata tcaatcttga tgtaaaattt
      601 atgcagggca ttatggcttc tacagaaatg cttgctgtgc aaatatggaa tcagttgtat
      661 gatgccatca atagtcaggg ggcaatgctt catgctgtta aactctatga aacagagaat
      721 aattttgttg aatattttgg aaacgaaaaa tgaataaaga cccacacact ataattgatg
      781 atgatgatga cccggagggc tatcgtaaga tagatcatta caatcatgat tcattaaata
      841 aattgtcaca aagttattta gaggtattac aaaatctggg agagaatact tcgcgcgaag
      901 gtattctcaa aacaccggag cgtgttgcaa aggcaatgca atttctcaca caaggttatc
      961 accttaatgc aaaagaaatt ttagagtcgg cacgctttcg cgaagactac aaacaaatgg
     1021 ttattgtaaa ggatattgaa gtatattctt tgtgcgagca tcacatgttg cccttttttg
     1081 gtaaagcaca tgttgcctac ataccaaaca actacattgt aggtctaagc aaaataccgc
     1141 gtgttgttga tgtctttgcc cgcagattgc aggtacaaga aaggcttaca gttgagattc
     1201 gcgatgccat tcaggaaacc ttatcacctt tgggagtggc agttgtcatt gaggcaaggc
     1261 acctttgcat gcagatgcgt ggtattcaaa aacaaaactc tgttacaaca acatctgcat
     1321 ttactggcga attcgaaaac gacagaacaa gaagtgaatt cttacgatta atagcagaca
     1381 agcatattta aagtacatga cgcagaaaaa gtttttggtt tttgttgttg aagatgaccg
     1441 tcatctacaa aaaatgatag tagattatct tacagagcat ttccctgata tagaaattaa
     1501 tctttataag acaggcgaag atgcactcat tgatttaaaa ttgggtcccg atttaattat
     1561 acttgattac ttcctgagtc tttacgataa agatgccatg gatggattaa cggtgctctc
     1621 acacatcagg caagccaaca aaactgtgcg cgttgttatg tttaccggac aggaagaccc
     1681 cgatgtagca gcttctgcaa ttaaaatggg cgcattcgac tacatagtaa aatcacctga
     1741 aagtttcaat aaactcagcg aaatcatcac acagctcaaa aaacaaattg taaacgaaac
     1801 gcctcctttt gacaaaagat tgctgatagt attgcttatc ggcatcatca tgctaagtgc
     1861 gttgtttata ttcaggcatt aaatatttca ttggaatatt tttaatcaat tgacggatga
     1921 agcttaaaat tataaaggat aatttactca aggcttatct cgggcaagtt ccaaaaggtt
     1981 tgcaaaaggc atttgataat ttgcaagatg cagaattgtc aactccgaat tttagttttt
     2041 atacttcagt agctactgta tttagcagta agatagaagg tgaatctata gagttagaca
     2101 gctacatcaa acacagacgt gatggcattt catttcagcc cgattacaca aagaagattg
     2161 atgatttgta taatgcttat ctctttgcac aagaaaataa attaaatgag caaaatgtaa
     2221 agcaagcaca tgcaatatta agtaagcaca ttgttacaaa tgaatggcag ggtaggtatt
     2281 ggcaacaaaa tatgtacgta acctctgaag acgggcgcat agagtatgta gctgcattac
     2341 ctcaaattgt agaggatgaa atattaaaac tttttgaaga cattgacttg ttgattaaaa
     2401 ccaatttatc tttagggaaa gtattttatt atgctgctat gatacattta gtctttgtaa
     2461 aaatacaccc ctggaatgac ggcaatggca ggagcgcccg gctgattgaa aaatggtttt
     2521 tatcagagaa gttgggtgca aaggcttggt ttatgcagag tgaaaaaatg tattattcac
     2581 gacacagtga gtattataat tgccttaaac aacttggttt ggagtatgaa aatctgaatt
     2641 accaaaaagc acttcctttt gtattaatgc tcccggattg tgtctgattt aaataactcc
     2701 tgaactgcat ttagaattat ttaatagggt ctttttatgt tttaagaaat gatgaattct
     2761 cagatttata attgaaattg tattttcgta gttatgaaac gcagcgactt tattaaaaat
     2821 ctaattggca ttggatcttc ggcattggta ctaccatctt atgcgtggat taagcaatac
     2881 acagcagcat caattgtaag tcagaacaaa caattgtgta tgcctgca
//
LOCUS       DAORQT010000200         2917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6756_length_2917_cov_7.313417, whole genome shotgun sequence.
ACCESSION   DAORQT010000200 DAORQT010000000
VERSION     DAORQT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6756_length_2917_cov_7.313417"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK134_06900"
     CDS             <1..475
                     /locus_tag="PK134_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRB38610.1"
                     /translation="RKLTASISRTKCCCIFINQLREKIGVMFGNPETTTGGNALKYYA
                     SVRLDIRRIGQIKDGDSVAGNRTRVKVVKNKVAPPFRQAEFDIMYGEGISKTGELVDL
                     GVELNIVKKSGSWFSYGDTKLGQGRDAVKQLFSDNPELADEIENKIKEAMNLKTA"
     gene            602..1597
                     /locus_tag="PK134_06905"
     CDS             602..1597
                     /locus_tag="PK134_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38611.1"
                     /translation="MTLKKHYILYFSVLIFTACTNGKKETEEPKDKIAQLTESIKADS
                     TNPKNYFDRATAYSQKSMFDLAYFDMEKAMKIKPSDPEYLMLYSDLSFRMNKVKDARD
                     ALVKVREMQPSNYDAAFKLAEIFLYTNNQDQSLHYLDTVLQKDPKNTKALLMQGFNLK
                     EKKDTAAAITAFRNAVAIDPKFYEAQIQLGLLMYLQNNKLCVDYYTNALNIKANSEEA
                     MYGLALWYQDHQEFNKAIQFYTDIIQINPKNKNAHFNLGYIHHEYLKVYDQAIKHYSD
                     AIAADPNYAEAYYNRGLCYEYVGNITAAKDDYSKALQIRKGNYPHAQEGLQRVSR"
     gene            complement(1628..2881)
                     /locus_tag="PK134_06910"
     CDS             complement(1628..2881)
                     /locus_tag="PK134_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38612.1"
                     /translation="MDLNSAGNFKWVKLAGPGPGTLGTGGRQIQITDDDMINVIGSGG
                     SGVIFPGYNYFSLSWCFTARFDTSGNINRFQNLGSVYGPFYRDCKISPTGDQYITGYF
                     VADSVVIGDSTLYLTNAPNVGIDLFLAKFDSSGNFKWVKQIGTPSANGMKGYGVDIIG
                     SDIVLTGMVHPGTIIGSTTITNPLYLLNSVVPFVARLYNQDSVVWVSSIQCSSAYGMP
                     TGGVSVYNNQIATAGMFLGKAVIGSDTITAVSYRDIFLSIMDASTGNFTKGVNLPGTG
                     SSEEPQCITHDSSGNIYVGGSYDGTLTVNGINYPYQGGNSDGFIAKWGSNCTVGLEEQ
                     VPLPAENLFLYPNPATSEIKIQNKEQLIVAIEIYDMVGKKIMYRKVSGKESTIQIDVS
                     SFSPGMYVVKTKGKTNMLTAKFVKE"
BASE COUNT          979 a          576 c          518 g          844 t
ORIGIN      
        1 gcgtaaactt actgcaagca tcagccgaac taaatgttgc tgtattttta tcaatcagct
       61 tcgcgaaaaa attggtgtta tgttcggaaa tcccgaaaca acaaccggtg gtaatgcatt
      121 gaaatattat gcatcagtgc gtttagacat tcgcagaata ggacagataa aagatggtga
      181 cagtgttgca ggaaaccgca cccgtgttaa agtggtgaaa aacaaagtag ctcctccatt
      241 ccgtcaggca gaatttgaca ttatgtatgg cgaaggtatt tctaaaacag gagagcttgt
      301 tgaccttggt gttgaattaa acattgtaaa gaaaagcggt tcgtggttca gctatggcga
      361 taccaaactt ggtcagggac gcgatgctgt gaaacaactt tttagtgaca atcctgaact
      421 tgccgatgag attgaaaata aaattaaaga agcaatgaat ttaaaaactg cttagaaaaa
      481 taattatttg tttattggta aaagaaaaga gctgccggtt ttcggtggct tttttcattt
      541 cataccaatt gtatatgatg gattgtgttt aaaccgatat ttgcaccaca ataacattag
      601 aatgacattg aagaaacatt acatacttta tttttcagtg ctgattttta ctgcctgcac
      661 aaatggaaaa aaagaaacag aggagccaaa agataaaatt gctcagctta cagaaagcat
      721 caaagccgac agcaccaatc caaaaaacta tttcgaccgt gccactgcct attcgcaaaa
      781 gagcatgttt gatttggctt attttgatat ggaaaaagca atgaaaataa aaccatctga
      841 cccggaatat ctgatgcttt attcagacct tagtttcaga atgaataagg taaaagatgc
      901 acgcgatgca ttagtaaaag tgcgcgaaat gcaaccatca aactatgatg ctgcatttaa
      961 attggcagaa atatttttat ataccaacaa tcaggatcag tcgttgcatt atttagatac
     1021 agttttacag aaagacccta aaaacaccaa ggcattgctg atgcagggct tcaacttaaa
     1081 agagaaaaaa gatacagccg ctgccattac agctttcaga aatgctgttg ccattgaccc
     1141 taagttttat gaggcacaaa tacaattggg tttactgatg tatttacaaa acaacaaact
     1201 gtgcgttgac tactacacca atgcattaaa cattaaagcc aacagcgaag aagccatgta
     1261 tggtctggca ttgtggtatc aggatcatca ggagtttaat aaagccatac agttttacac
     1321 cgacattatt cagataaatc ctaaaaacaa aaatgcacat ttcaaccttg gatacataca
     1381 tcatgaatat ctgaaagttt acgatcaggc aatcaaacat tattccgatg ccattgcagc
     1441 agaccccaat tatgcagaag cctactataa ccgtggttta tgttatgagt atgttggaaa
     1501 tattacagca gcaaaagacg attacagcaa agcactgcaa ataagaaaag gtaattatcc
     1561 acatgcacag gaaggactgc aaagagtgtc gaggtagagg agatgaattt ttgagacaca
     1621 ttctacccta ctccttcaca aactttgctg ttagcatatt tgttttgccc ttggttttta
     1681 ctacatacat gcccggtgaa aatgacgaaa catctatctg aatggtagac tctttaccag
     1741 aaacttttct gtacattatt tttttaccaa ccatatcata aatttctata gcaacaataa
     1801 gttgctcttt attttgaatt tttatttctg atgttgcggg gtttggatat aaaaataaat
     1861 tttctgcagg caatggcact tgttcttcta aacctaccgt acagttgcta ccccacttag
     1921 caataaaacc atcggagttg ccgccttggt agggatagtt tattccattt acggttaaag
     1981 tgccatcgta actaccacca acataaatat tgccgctgct atcgtgagtt atacactgtg
     2041 gctcttcact actgcctgta cccggtaaat ttacaccttt tgtgaaattg cccgttgagg
     2101 cgtccattat tgataaaaat atatctctat aacttactgc tgtgatagta tcgctcccta
     2161 taactgcttt tcctaaaaac attcccgcag ttgctatttg attgttatat acagaaacac
     2221 cacctgtggg catgccataa gcagatgaac actgaatgga cgaaacccac actacactat
     2281 cttgattata taacctagct acaaaaggca ctacagaatt taaaaggtat aatgggtttg
     2341 taattgtagt tgatcctata attgtgccag ggtgtaccat gcctgttaaa acaatatcag
     2401 agccaataat atctactccg tagcctttca ttccattagc tgatggagta cctatttgtt
     2461 tcacccattt aaagttacct gaggaatcga atttagccaa aaacaaatct attccaacat
     2521 ttggtgcatt agttaagtac aaagtagaat caccaattac aacagaatct gcaacaaaat
     2581 agcctgtaat gtactgatcg cccgtgggac ttattttgca atctctataa aatggaccat
     2641 atacactgcc taaattctgg aatctgttaa tatttcctga agtatcaaaa cgcgcagtaa
     2701 aacaccaact aaggctaaaa taattatagc cgggaaaaat aactcccgat cctcctgaac
     2761 cgataacatt aatcatatcg tcatcagtaa tttgaatttg tcttccgcct gtacccaaag
     2821 ttcccggtcc cggacctgct aatttaaccc atttaaaatt tcctgccgaa ttcaaatcca
     2881 ccgcaactac ttaccggaat attatcaacc tacagtg
//
LOCUS       DAORQT010000201         2908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6794_length_2908_cov_5.393270, whole genome shotgun sequence.
ACCESSION   DAORQT010000201 DAORQT010000000
VERSION     DAORQT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_2908_cov_5.393270"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK134_06915"
     CDS             <1..236
                     /locus_tag="PK134_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38613.1"
                     /translation="VLAAAEIFKSIERKETYKIALEYHPVKALYVRGGVSTEPVQLTF
                     GFGLKFSQFYADFTSGYFEPLGYSPGLALRYAF"
     gene            239..2308
                     /locus_tag="PK134_06920"
     CDS             239..2308
                     /locus_tag="PK134_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB38614.1"
                     /translation="MKTIQLFFISVLFCNITLAQVVPPQDDSKIEEKVENIANTADES
                     ADLTELTEQLQQLAANPINLNKATREDLLQTGLFSEVQADAIIEHREISGKFISLYEL
                     QAIDFLSLEDIEEILPYVTVGNTEDIKATLKKIFTDGNSRLIVRGQRYLEKQKGFRDS
                     TATDKVHPAYPGSPWKLYTQYQYRYKQRFSFNLTAEKDQGEEFFKGSQRNGFDYYSAH
                     LAIRDFGIIKRAVIGDYDLFYGQGLTLCTGLSFGKSPDVAAVRKFSTGIRPYTSLNES
                     SFKRGVAVSVGKRNWNADAFYSNRKLDANLNSILDTTLEQQDIFTSFIESGYHRTKSE
                     LENKNTIREQMYGVNAGYNTRNFTAGLTAVHAGYDRPLQRSFQLYNRFDFSGKQFTKV
                     GIDYSWLYRNINFFGEFSRSDNGAIAYVNGAIISLGTTVSVSVVNRNYPRDFNFLYAS
                     GFGESSTTRNEKGTYAGIVIRPNRYWGFNGYLDRFTFPWLRFGADAPSKGYEYLWQVS
                     WNPSRQFEIYFRNRRTQKEENSSAAAVADYLVFKRQDNYRFNLTYKISKAVAIRNRIE
                     WVRINYEEKPNEKGFMIYQDILFKPLSSPISGSVRYGLFDTDSYDSRIYAFENDILYS
                     YSIPSFYNRGFRYYVTLRYKVTKGVDVWFRFAQTVYTNQKTFGSGLDEINNNKRSEIK
                     LQVRYQF"
     gene            2322..2837
                     /locus_tag="PK134_06925"
     CDS             2322..2837
                     /locus_tag="PK134_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38615.1"
                     /translation="MRIWGLKLLVLIWVVFTGYWLLPLKKPKAYRQDSEPNTILATQQ
                     FCEPECADIIIKKGELIIPDSLKKMYPELRKDVALLVGSSPFKKSKSALMFSYDFVLS
                     GHVERVGFTQQEGFVPVFHVEEWFPTQYIARFWKLTHNSELLYLINLNIGLPLLLFYF
                     FKQGSSLNKFF"
BASE COUNT          919 a          492 c          572 g          925 t
ORIGIN      
        1 aagttctggc agcagcagaa atttttaaat caattgaaag aaaagaaact tataaaattg
       61 cactggaata tcatcctgtt aaggcattgt atgttcgtgg tggcgtgagt acagaaccag
      121 ttcagcttac atttggtttc ggattaaaat tttctcaatt ttatgctgac tttacttcag
      181 gttattttga acctttaggt tacagtccgg ggttggcttt gcgttatgct ttttaattat
      241 gaaaacaatt caactgtttt ttatttcagt attattctgc aacattacac ttgcacaggt
      301 ggtgccacca caagatgaca gtaagattga agagaaagtt gaaaacattg ccaatacagc
      361 agatgagtct gctgacctga ctgaactgac agagcaactt cagcaattag ctgccaaccc
      421 aataaatctg aataaagcta cacgtgaaga tttgttgcag acgggattgt ttagcgaagt
      481 gcaggcagat gctattattg aacaccgtga aatttcagga aaatttattt cgctttacga
      541 acttcaggca atagactttc tttcgttaga agacattgaa gaaattttac cttatgtgac
      601 agttggaaat acagaagaca taaaagcaac cttaaaaaaa atatttactg atggcaacag
      661 cagacttatt gttcgtggcc aacgttatct cgaaaaacaa aaaggctttc gcgacagcac
      721 agcaacagac aaagttcatc ctgcctatcc cggcagccca tggaaacttt atacacaata
      781 tcagtatcgc tacaaacaaa gattcagttt taatttaaca gcagaaaaag atcagggaga
      841 agaatttttt aaaggctcac aaagaaatgg ttttgattat tactctgcac atttagctat
      901 tcgtgatttc ggaattataa aacgtgctgt tattggtgat tatgatttat tttatgggca
      961 aggattaaca ttatgtacag gtttgtcatt cggtaagtcg cccgatgttg ctgctgtacg
     1021 taaattttct acaggtatta ggccttatac ttctctaaac gaatccagtt ttaaaagagg
     1081 tgtagcagtt tctgttggta aacgaaattg gaatgccgat gctttttata gtaacagaaa
     1141 attagatgca aacctgaata gcattttaga taccacgctt gagcaacaag atatttttac
     1201 ttcgtttatc gaatcaggct atcatcgcac caaaagcgaa cttgaaaaca aaaataccat
     1261 tcgcgaacaa atgtatggtg tcaatgcagg ttacaataca cgtaatttca cagcaggatt
     1321 aacagcagtt catgcaggtt acgacagacc tttgcaacgc tcgtttcaac tttacaatcg
     1381 ttttgacttc agtggaaaac aatttaccaa agtgggcatt gactacagtt ggttgtatcg
     1441 caacatcaat ttcttcggag aattttcaag aagtgataat ggtgcaattg cttatgttaa
     1501 tggtgccatc atcagtttag gaaccaccgt tagtgtttct gtagtgaaca gaaactatcc
     1561 tcgtgacttt aattttctct atgcaagcgg atttggtgaa tcaagtacta caaggaatga
     1621 aaaaggaact tatgcaggta ttgtaatcag accaaatcgc tactggggtt ttaatgggta
     1681 tttagatcgt tttacatttc catggctccg ttttggtgcc gatgcacctt ctaaaggtta
     1741 tgaatattta tggcaggtgt catggaatcc gtcaaggcaa tttgaaattt atttccgaaa
     1801 caggcgcaca caaaaagaag aaaacagcag tgcagcagca gttgcagatt atcttgtgtt
     1861 taaaaggcag gataactatc gtttcaacct tacttacaaa atttctaaag ctgttgctat
     1921 cagaaatcgt attgaatggg tcagaattaa ttatgaagaa aagcccaatg aaaaaggatt
     1981 tatgatttat caggatattt tatttaaacc gcttagctcg cctatttcag gctctgtgcg
     2041 ctatggatta tttgatacag acagctacga ttcgaggatt tatgcttttg aaaacgacat
     2101 tctctattct tattctattc catcattcta taaccgtggg tttcggtatt atgtaacact
     2161 tcgctataaa gttaccaaag gtgtagatgt ttggttccgt tttgcacaga ctgtttacac
     2221 caatcaaaaa acatttggct cagggcttga tgaaataaat aataataaaa gaagtgaaat
     2281 aaagttacag gtaaggtatc agttttagta tttttgcagt gatgaggatt tggggtttga
     2341 aattattggt tttaatatgg gtagtattta ccggatattg gttgctgcca ttgaaaaagc
     2401 ctaaagcata tcgccaagat agcgagccta atactatttt agcaacacag caattttgcg
     2461 aaccggaatg tgccgatatt atcattaaaa aaggtgagtt aattatccct gatagcttaa
     2521 aaaaaatgta tcccgaatta cgaaaagatg ttgctcttct cgtaggaagt tcacctttta
     2581 agaagtcaaa gagtgcactc atgttttcat acgactttgt cttgtccggc catgtagagc
     2641 gtgttggttt tacacagcag gaaggttttg ttccggtttt tcatgttgaa gaatggtttc
     2701 ctacacaata tattgcaagg ttctggaagc ttacgcataa cagtgagttg ctgtatctga
     2761 taaatcttaa tatcggactg ccgttgttgt tattttattt tttcaagcaa ggttcatcac
     2821 tcaacaagtt tttctaaact gtcagatttt cttacaatga atttatgctg acgatttagc
     2881 atcaagaaaa acagcgcctt cattttta
//
LOCUS       DAORQT010000202         2907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6798_length_2907_cov_5.406381, whole genome shotgun sequence.
ACCESSION   DAORQT010000202 DAORQT010000000
VERSION     DAORQT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6798_length_2907_cov_5.406381"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK134_06930"
     CDS             complement(<1..807)
                     /locus_tag="PK134_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB38616.1"
                     /translation="MIINIVKSVASVVGLMVTKIPKTDYKEELYKSLFNNDSLAYKRF
                     YNVGAGPFNHKYWTNGDYVNDWYADNTNNTLKGINYDLFALEPLPIETDSAELVYTSH
                     TIEHVNNAAVQNLFNESFRVLKPGGRVRVVTQDIKLSYRAYKENDRHFFFWIEWFSKP
                     ENYKRVNLRQPLSQESIAQIFLEDFAAQASEIPLHGAKHRISDSELEKLFEEKSFEDA
                     LDYCTSLCDIEVQKKYTGNHINWFTEEKLIRMLKAAGFKSIYRSGYGQSYS"
     gene            complement(875..>2907)
                     /locus_tag="PK134_06935"
     CDS             complement(875..>2907)
                     /locus_tag="PK134_06935"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38617.1"
                     /translation="SNGVPVTSYTWTVAPGVTLVGQGNDTVQITFPINYNYTDIYVKA
                     NNGCGSSSNRSVRVRATPETPQPIIGLASGICNGTTPYTVPSSPTAVSYNWTLTGPGT
                     ILTGQGTNNINIDYPNTFISGKICVTITNACATGSPRCLLTSKNIALSEQPQDFETCN
                     STQAMFSVGALGMNLQYQWRKNGAPLSNGGKISGANSDTLYITGADSTDADQYDVIVS
                     NNCGSSITSNTATLDIKQVPAKPGAISGVQATTCPGTSGIIYSVPLQMDATSYLWTYT
                     DGVQILNGQGTDAVTLAFDSTINSGYSVFVYAVNECGQSLDSASSWTRYKVSTPTITG
                     PARVCENLPGVNYMAPAVAGASSYNWTIPAGATLVSGQTTNAIVVDFGPTYTGGDVTV
                     TASNICFTTPVKKLTTVIDMPSVPTSLSGQVYGTCKTQLVYTAGNSNHATSYKWTLPP
                     NSSIILGNFTNTVTVDFQNPGSGTYSLCVAGNNACRTGTYRCIQVRAIPERPASIVAN
                     PSVFCANQTGVAFSTAGSVGADSYTWALPSTSTITSGQNTSSILADIGTNNGTITVMG
                     VNQCGNSGTRTYAMTLNCREGFDKSELVQSVLLAPNPANDWVKLSFNSETAENYSIEV
                     FDILGKKVLSEGGISSKGLNEHSILINKLVKGIYIVSLQSNSVSSRQKLEIR"
BASE COUNT          876 a          571 c          495 g          965 t
ORIGIN      
        1 tgagtaactt tgtccataac cggaacgata tatactttta aatccggctg cctttaacat
       61 tctaataagc ttttcctctg taaaccagtt aatgtgatta cccgtgtatt tcttttgtac
      121 ttcaatatcg cataatgaag tacaataatc cagagcatct tcaaaggatt tctcttcaaa
      181 caatttttct aattcactat cggagatacg atgtttagca ccatgtagag gaatttcaga
      241 tgcctgtgct gcaaaatctt caagaaaaat ttgtgctatg ctttcctgac ttaaaggttg
      301 cctgagattt actcttttat aattttcagg cttactgaac cattctatcc aaaagaaaaa
      361 atgcctgtcg ttttctttat aggctctgta tgataactta atatcctgtg tgacaactct
      421 aactcttcct ccgggtttta atactctgaa agattcgtta aacaaattct gaaccgctgc
      481 attatttaca tgctcaatgg tatgactggt atataccaac tctgcagaat ctgtttcaat
      541 tggcaatggc tctaatgcaa ataaatcata gttaatgcct tttaatgtat tgtttgtatt
      601 atcggcatac caatcattca cataatcacc attggtccaa tacttatgat taaatggccc
      661 tgcccctacg ttataaaatc gcttatacgc aagactatca ttattaaata aactcttata
      721 aagttcttct ttataatcag tcttaggtat tttagttacc atcaaaccaa ctacacttgc
      781 aacagatttt actatgttta taatcataaa tatttattta aattgcaaag tcgctttttt
      841 aaagcgactt tgcaattacc tccaataaat aagactatct tatctcaagt ttttgtctgc
      901 tcgagacaga attactttgc aatgaaacaa tatatatacc tttcactaat ttattaatca
      961 aaattgaatg ttcattcaat ccttttgatg aaattccgcc ttcgctaagg actttctttc
     1021 caagtatatc aaatacttct attgagtagt tctctgcagt ttcagaatta aaggacaatt
     1081 taacccaatc atttgccgga tttggagcta acaatactga ctgtacaagt tctgatttat
     1141 caaaaccttc acggcaatta agtgtcatag cataggtacg tgtgccactg tttccacatt
     1201 gattaacacc cattactgtt atagttccat tatttgttcc aatgtcagct aaaatactac
     1261 ttgtattttg tccggatgta attgttgagg tacttggcag tgcccaagta tatgaatcag
     1321 cacctacact tccggcagtt gagaatgcaa caccggtttg gttagcacag aaaactgatg
     1381 gatttgctac aatacttgct ggtctttcag gaatagcacg cacctggatg caacgatatg
     1441 ttccggttct acaagcatta ttacctgcaa cacaaagact atatgtaccg cttccgggat
     1501 tttggaagtc aactgtaaca gtatttgtaa aatttcctaa aattattgaa gagttaggtg
     1561 gtaaagtcca tttataactt gttgcatggt tagaatttcc tgcagtataa actaattgtg
     1621 ttttacaagt accatatacc tgaccactta aggatgttgg aactgatggc atatcaatta
     1681 ctgttgtcaa ctttttaact ggtgttgtaa aacagatatt agatgcagta actgttacat
     1741 caccaccagt gtaggttggc ccaaagtcca caacaattgc attagttgtt tgtccactaa
     1801 ctaaggttgc tccggcagga atggtccaat tatacgaaga tgctccagcc actgcaggtg
     1861 ccatataatt tacacccggt aaattctcac aaactcgtgc aggacctgtg attgtaggtg
     1921 ttgatacttt atagcgagtc caggaactgg cagagtctaa actttgtcca cattcattta
     1981 cagcataaac aaaaacagag tatccggaat ttattgttga atcaaaagct aaagtaacag
     2041 catcagtacc ttgaccattt aatatttgta caccatcagt atatgtccac aaataactgg
     2101 ttgcatccat ttgtaatgga acagaataaa ttattccaga tgtaccggga caagttgttg
     2161 cctgaacacc acttattgca cccggcttgg cagggacttg cttaatatct aatgtagctg
     2221 tatttgatgt gattgatgat ccacaattat tggatacaat gacatcatac tgatcggcat
     2281 ccgtactatc agcaccggta atatataatg tatcactgtt tgctccggaa attttaccac
     2341 cattacttaa tggagcacca tttttacgcc attggtattg caagttcatt cctaatgctc
     2401 caacagaaaa cattgcttgc gtactgttgc aagtttcgaa atcctgaggc tgttcgctta
     2461 aagcaatatt ttttgaagtt agtaaacaac gcggacttcc tgttgcgcac gcattagtga
     2521 tagtaacaca aattttacct gaaataaatg tatttgggta atcaatatta atattgtttg
     2581 ttccttgtcc ggttaaaata gttcccggac ctgttaatgt ccaattatat gaaaccgcag
     2641 ttggactaga aggtacagta taaggagttg ttccattgca aattcctgat gcaagaccta
     2701 tgataggttg cggtgtttcc ggagttgctc tgacacgcac acttctgtta gaacttgaac
     2761 cgcatccgtt atttgctttc acataaatat cggtatagtt gtagttaatt ggaaaagtta
     2821 tctgaacggt atcatttcct tgtcctacaa gagttacacc cggagccaca gtccatgtat
     2881 aagaagttac aggtaccccg tttgatg
//
LOCUS       DAORQT010000203         2865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6936_length_2865_cov_8.892171, whole genome shotgun sequence.
ACCESSION   DAORQT010000203 DAORQT010000000
VERSION     DAORQT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_2865_cov_8.892171"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="PK134_06940"
     CDS             <1..113
                     /locus_tag="PK134_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRB38618.1"
                     /translation="FGNCTNTGACEAECPKEISIENIARMNREYLSAKIS"
     gene            222..932
                     /locus_tag="PK134_06945"
     CDS             222..932
                     /locus_tag="PK134_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRB38619.1"
                     /translation="MTASEEERIRKAFSDKDWQEIKAQDTWQIFKIMSEFVEGFEKMS
                     RIGPCVSIFGSARTKPDTRYYLLAEEIAYQLTQSGYGVITGGGPGIMEAGNKGAQRAG
                     GKSVGLNIKLPFEQTSNPFVDHDKNLMFDYFFVRKVMFTKYAQGFVVLPGGFGTLDEF
                     FEALTLIQTKKIGRFPIVLVGKEYWGKLFEWLQTVMVDEGNILPEDLSLFKLVDEAHE
                     AKSHIDAFYSKYLLKPNF"
     gene            complement(929..1693)
                     /locus_tag="PK134_06950"
     CDS             complement(929..1693)
                     /locus_tag="PK134_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRB38620.1"
                     /translation="MLTSSEIKQIKSLKDKASRHEQQKFVVEGEKMVTELAHSAFNII
                     AVYATDEWIGQANNEILSLLNEKLIQVKTKELERISSLKQPNGVLALVAMRQQQETEI
                     KFNDIILAFDDISDPGNLGSIIRIADWFGIKQIVCSSTSVDLYNSKTIQATMGSIFRV
                     NVFYTDVEKWISANKQNATFYATMLNGKSVYSVAKKKPAVLVFGNESRGLSHSIQQLI
                     DLDISIPKFPDEKSSSVESLNIAMSAAIFCSEWRRD"
     gene            1837..>2865
                     /locus_tag="PK134_06955"
     CDS             1837..>2865
                     /locus_tag="PK134_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38621.1"
                     /translation="MDKGEYLLNRNQVIIDKPELREGVKAIIKQKANRRILGLFRFHL
                     AVYSMGNRGKPTKFKNWLKNTVGEEPEVLDTLFTKKSTQQIKQFMSKNGYFDAEVKDS
                     TIYRKKKAIVRYTIVAHKPSFIKYVARECADDNLDSLVIKDTSTSKLQAGIIYNEGIL
                     QGDRERLTKKLRNEGYFQFSPQFVSYRVYTSDTTKNIAVFQDVAAFSNPDSTISSAAL
                     PHTKFKLNRIIFSPDYDPLSLNTSLIQYDTVAYKGYLFVKRAGKRMPVKPEVIVNHTF
                     LKPEAVFRQSNADYTYKGLNLLGMYRFVNINYNVVDSNRLDCFINLGTTPKQEYKTEL
                     EGTNNGGNL"
BASE COUNT          960 a          495 c          533 g          877 t
ORIGIN      
        1 gttttggcaa ttgtaccaac acaggcgcct gcgaagcaga gtgtccaaaa gaaatttcta
       61 ttgaaaatat tgcccgtatg aacagggaat atctttcagc aaaaatttct taaacagcat
      121 ctactgaact ttttttagca ttattcgtaa aaacaaacga acgggtaatt tattaccgga
      181 tggttctttt tctaagaatc attttttaaa aataaaatcg catgacagca tcagaagaag
      241 aaagaatcag aaaagcattt tcagataaag actggcagga aattaaagct caggatacct
      301 ggcagatttt taaaataatg agtgagtttg ttgaagggtt tgaaaaaatg agccgcatag
      361 gcccttgtgt ttcaatattt ggttcggcaa gaacaaaacc agatacaaga tattatttac
      421 tggcagaaga aatagcttat caactcacac aatccggtta tggtgtcata acgggaggag
      481 gcccgggtat tatggaagca ggtaacaaag gtgcacaacg tgccggtgga aaatctgttg
      541 gtttaaatat taaacttccg tttgaacaaa catcaaatcc ctttgtagat catgataaga
      601 atctgatgtt tgattacttc tttgtacgca aggtaatgtt taccaaatat gcccagggtt
      661 ttgttgtact tcccggtggg tttggtacgt tagacgaatt ttttgaagcg ttgacactta
      721 ttcagacaaa aaagattggc agatttccga ttgtacttgt tggcaaagag tattggggta
      781 aattgtttga atggctgcaa acagttatgg ttgatgaagg caatatttta cctgaagacc
      841 tttcactctt taaattagtt gatgaagcgc atgaagcaaa gtcgcatatt gatgcattct
      901 acagtaaata tttactgaaa cctaactttt aatccctgcg ccattcactg caaaaaattg
      961 cagcagacat ggcaatattt aaagactcta cagagctgct tttttcatca ggaaactttg
     1021 gaatggaaat atccagatca atcaattgct gaattgaatg cgataaacct cttgattcat
     1081 ttccgaaaac caaaacagca ggttttttct tagcgacaga ataaacagac ttgccattaa
     1141 gcatggtagc ataaaaagtg gcattttgtt tattggcaga aatccatttt tcaacatctg
     1201 tataaaaaac attcactctg aaaatagagc ccattgtagc ctgaattgtt ttggaattat
     1261 aaagatcaac agaagttgat gaacatacta tttgcttaat accaaaccaa tctgcaattc
     1321 tgataatact acccaaatta ccggggtcgc tgatgtcatc aaatgccaga atgatatcat
     1381 taaattttat ttcggtttcc tgttgttgcc gcatggctac taaagccaaa actccattag
     1441 gttgttttaa tgatgatatc cgctctaatt cttttgtttt aacctgtatg agtttttcat
     1501 ttaatagact taatatttca ttatttgctt gtccaatcca ctcatcagta gcataaacag
     1561 ctataatatt aaaagctgaa tgagccagtt ctgtaaccat tttttcgccc tcaacaacaa
     1621 atttttgttg ctcatgacgg cttgccttgt cctttaatga ctttatctgt tttatttctg
     1681 aacttgtcag cattagaaga atactgttaa agggtataaa gatagatgat caataagttt
     1741 tatttttacc gcatctgaaa aaatatgcgc aaaaggcagt acggcatttt aattactctg
     1801 attttactgg tagcatcttg cagccccttt aagcaattgg acaaaggcga gtatttgctg
     1861 aatcgcaatc aggtaataat agacaagccc gaactgcgcg aaggtgttaa agcaattatc
     1921 aaacaaaaag ccaataggag aattttaggg ttgtttcgtt ttcatcttgc tgtatatagt
     1981 atgggtaatc ggggtaagcc aacaaaattt aaaaattggt taaagaatac ggtaggagag
     2041 gagcctgaag ttttggatac gctgtttacc aaaaagtcaa cacaacagat caaacagttt
     2101 atgtctaaga atggctattt tgatgcagaa gttaaagaca gtaccattta cagaaagaaa
     2161 aaagctattg ttcgttacac cattgttgca cataaacctt cttttataaa atatgtggca
     2221 agagagtgtg cagacgataa cctcgattct cttgtaataa aagatacttc aacctcaaag
     2281 ctgcaagcag gcatcattta taatgaaggc atattgcaag gcgaccgcga acgactgaca
     2341 aaaaaacttc gcaacgaagg ttattttcag ttttcaccgc agtttgtttc ttatcgtgtt
     2401 tatacatcag atactacaaa aaatatagcc gtatttcagg atgtagcagc attttcaaac
     2461 cccgattcaa caataagttc cgctgcatta ccacatacaa agtttaaatt gaatcggata
     2521 attttcagcc cagactatga tccattaagt cttaatacat cattaattca atatgataca
     2581 gtagcctata aaggttattt gtttgtgaaa cgagcaggaa agagaatgcc cgtaaaaccg
     2641 gaggtaatcg ttaatcatac atttcttaaa cccgaagctg ttttcaggca gagtaatgca
     2701 gactatacct ataaaggact taacctgtta ggtatgtatc gttttgtaaa catcaactat
     2761 aacgttgttg acagcaatag acttgattgt tttattaatc ttggtacaac gccaaaacaa
     2821 gagtataaaa cagagcttga aggtaccaat aacggtggca accta
//
LOCUS       DAORQT010000204         2859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_6955_length_2859_cov_5.612696, whole genome shotgun sequence.
ACCESSION   DAORQT010000204 DAORQT010000000
VERSION     DAORQT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_2859_cov_5.612696"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK134_06960"
     CDS             <1..467
                     /locus_tag="PK134_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38622.1"
                     /translation="PEGLVMKIWLDKLTGDVEVINGLNHYIGMKKISVEMFPEFAFLK
                     YIVAAFIVYGLVIAITGSRKILFSYLVVSVICGILALYDFYQWGYDYGHNLDPNAPIK
                     IPGLVYQPPILGHKQLLNFDAHSMPDVGGYIVIGSALICAIIWAMGLRKSKS"
     gene            480..869
                     /locus_tag="PK134_06965"
     CDS             480..869
                     /locus_tag="PK134_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRB38623.1"
                     /translation="MRWTFIFISLLIVSCTTKPEPLVAGKDACVHCKMPIADTRFGAE
                     IISNKGRIYKFDDINCMLQWSRQSSNVDAGSAKLYVVNYFDQIDLMNAADAVYLKSDN
                     LRTPMNSGFAAFATKEEAEKLLKDDIS"
     gene            887..2146
                     /gene="nosD"
                     /locus_tag="PK134_06970"
     CDS             887..2146
                     /gene="nosD"
                     /locus_tag="PK134_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRB38624.1"
                     /translation="MKLNRALFVVVKCFLILLFFSGKSQAGVLKIGKKFSIKSIKEGV
                     AKAKPFDTLFIHSGLYKEQNILVEKTLSLIGVDNPVLDGENKFEVLTIDANSVLVTGL
                     TIKNSGYSSMKDLAGIKVMNSRQFLIKNNTLINSYFAIHIANCDNFRIENNTIRGTPG
                     TDEQSTGNAIHLWKCQYASIKNNDISGHRDGIYFEFVENSHIEKNNSYNNLRYGLHFM
                     FSNKDSYIDNKFTDNGAGVAVMFSNHISMYYNLFSMNRGASAYGLLLKEISDGDIKDN
                     MFENNTTGVLMEGTNRLHITKNSFYKNGYALRVQASCSNNTVHDNNFNGNTFDVATNG
                     SLVLNNFDGNYWDKYEGYDLKRDGVGDVPYRPVSLFSMVTENIPIASIFIRSLIVFLL
                     DRSEKIIPSITPENLVDNKPLMKPVAL"
     gene            2143..2856
                     /locus_tag="PK134_06975"
     CDS             2143..2856
                     /locus_tag="PK134_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB38625.1"
                     /translation="MIETININKTFGKLRVLKDISIKLENNSSVALLGPNASGKTTFI
                     KSVLGMVLPESGTIKVNNTDIKNDDSYRSQIGYMPQIGRYPENMNIGQVFEMIMDMRK
                     EQMNHADTDLLDAFNMKSIFHKRMGTLSGGTRQKVSACLAFLFRPKIIILDEPTAGLD
                     PLSSEILKEKVKTEKNNGNLIIITSHILSELDELVSEIIFFREGELMFHKKLEELQLQ
                     TGEKSVSKVITSILKNNNE"
BASE COUNT          977 a          430 c          534 g          918 t
ORIGIN      
        1 atcctgaagg gttggtaatg aaaatatggc tcgataaact aacgggtgac gtagaggtta
       61 ttaatggttt gaaccactat ataggcatga aaaaaattag cgtagagatg tttccggaat
      121 ttgcattcct gaaatatatt gtagctgcat tcattgttta tgggttagtg atagccatta
      181 ccggtagtcg taaaatattg tttagttacc tcgtagtttc tgttatatgt ggcattcttg
      241 cgctctacga tttctatcag tggggttatg attatggaca taatcttgat cctaatgcac
      301 ctattaaaat tcccggatta gtttatcagc ctccaatttt aggacataaa caattgttga
      361 attttgatgc acactctatg cctgatgtag gtggatatat tgtcattggc tctgcactta
      421 tctgtgctat tatctgggca atgggattaa gaaaaagtaa atcttaaata ataaagtgta
      481 tgaggtggac attcattttt atttctcttc ttattgtatc atgtacaaca aagcctgaac
      541 cattagttgc cggtaaagat gcttgtgtac actgcaaaat gcccatagca gatactcgtt
      601 ttggtgcaga aataatatca aataaaggac gtatatataa atttgatgat attaactgca
      661 tgttgcaatg gagcagacaa tcatctaacg tagatgcagg ttctgcaaaa ttatatgttg
      721 taaattattt tgatcagatt gatttgatga atgcagctga tgccgtctat ttaaaaagcg
      781 acaaccttcg tacgccaatg aatagcggct ttgctgcttt tgctactaaa gaagaagccg
      841 aaaaactatt gaaagatgat attagttgac tggaattttg ttcagaatga aattaaatag
      901 agcactgttt gttgttgtaa aatgttttct gattcttctg tttttttcag gcaaatcgca
      961 ggcaggtgtt ttaaaaatcg gaaaaaaatt ttcaattaag tccattaaag aaggggttgc
     1021 taaagctaaa ccttttgata cactttttat ccattcaggt ctttataaag agcaaaacat
     1081 cctagtagaa aaaacgctgt cactcattgg tgttgacaat cctgtccttg atggagaaaa
     1141 taagtttgag gtcttaacaa ttgatgcaaa cagtgtatta gttaccggac taacaattaa
     1201 aaattcaggt tattcctcta tgaaagattt ggccggtatt aaagtcatga acagccggca
     1261 gtttctaatt aaaaacaata cgctcattaa ctcttatttt gcaatacata ttgccaattg
     1321 tgataatttc agaatagaaa ataataccat tcgtggaaca ccgggaactg atgaacaatc
     1381 taccggcaat gctattcatc tttggaagtg ccagtatgca tctataaaaa ataatgacat
     1441 ttctggtcat agagatggta tatattttga atttgtagaa aactcacata ttgaaaaaaa
     1501 taacagttat aataatttac gttatggctt acattttatg ttctcaaaca aagattcata
     1561 catagacaat aaatttactg ataatggtgc tggtgttgct gtcatgtttt ccaatcatat
     1621 ttcaatgtat tataatttat ttagtatgaa cagaggagct tctgcctatg gtttgttgtt
     1681 aaaagaaata tctgacggag atataaaaga caacatgttt gaaaacaaca caaccggagt
     1741 tttgatggaa ggaacaaaca gacttcatat cacaaaaaac agtttctata aaaatggata
     1801 tgccttgcgt gttcaggcaa gctgtagtaa caacacagtt cacgataata attttaacgg
     1861 aaacacattt gatgttgcaa ccaatggctc attggtttta aataactttg atggtaacta
     1921 ctgggataaa tatgaaggtt atgatttgaa aagagatggt gttggagatg taccctacag
     1981 accggtaagt ctgttttcga tggttacaga aaatattcct attgcttcaa tatttataag
     2041 aagtttaatt gtgtttttac ttgaccgcag cgaaaaaatt attccaagca ttacaccgga
     2101 aaatttagta gataataaac cattaatgaa acctgtagca ctgtgataga aaccataaac
     2161 atcaacaaaa cttttggtaa actcagggta ttaaaagata tatcaataaa gcttgaaaac
     2221 aacagctctg ttgcgttact tggacctaat gcttctggta aaacaacatt tattaaatct
     2281 gttttaggaa tggtgttgcc cgaatcgggc acaataaaag ttaacaacac agatattaag
     2341 aatgacgaca gctatcgcag tcagattgga tatatgccgc agattggtcg ttatcccgaa
     2401 aatatgaata ttggtcaggt gtttgaaatg attatggata tgcgtaaaga gcaaatgaat
     2461 catgcagaca ctgacttact cgatgcgttt aatatgaaat ctatttttca taaacgtatg
     2521 ggaactttat caggtggcac aagacaaaaa gtgagtgcct gtcttgcatt tctgtttcgt
     2581 cctaaaatta ttattcttga tgaaccaact gccggactcg accctttatc atcagagata
     2641 cttaaagaaa aagtcaaaac agaaaaaaat aatggcaatc tgataattat tacatcacac
     2701 attcttagtg aacttgatga gttggtttcg gaaattatct ttttcaggga aggggaattg
     2761 atgtttcata aaaaattaga agagcttcaa cttcaaacag gggagaaatc tgtttctaaa
     2821 gttattacat ccattttaaa aaacaataat gaataagat
//
LOCUS       DAORQT010000205         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7018_length_2842_cov_5.854324, whole genome shotgun sequence.
ACCESSION   DAORQT010000205 DAORQT010000000
VERSION     DAORQT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7018_length_2842_cov_5.854324"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>645)
                     /locus_tag="PK134_06980"
     CDS             complement(157..>645)
                     /locus_tag="PK134_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB38626.1"
                     /translation="NIGVNGAEYKSFLGQPMFFEQVKELNADLYILSFGTNEAFAKYF
                     DGTVFIQTVDSFITKLRNVSPTASILITSPTESCKRIRRGVYVPNPNVIAIKNLLQKY
                     CMDNKIAFFDLYECSGGKGSMLRFAKNKMSDARRIHLNRSGYEMQGLMLLQALLKPLS
                     EQ"
     assembly_gap    646..676
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<677..1408)
                     /locus_tag="PK134_06985"
     CDS             complement(<677..1408)
                     /locus_tag="PK134_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38627.1"
                     /translation="MKRIILIFSLLIYCTKCHAQSDSDTTMPVLQYEKFVEYKMSLLK
                     FLKPDAIDIEQKEALKNFYSKLLQSRADGKKVSIVHIGDSHLQAGYFSGMVRRGLQQR
                     YGNAGRGIVFPYKLARSNGPHDYVSFSQNSWKGNRNVITQDSLSTGITGFSVTTNDSS
                     PSVGISLRNNEGIDYRFNRVQVFYKPDSTFMTASDSLAIIETVNPIKLATGINEFRFS
                     DYLSNCKINISSDTTYTLFGLNLLND"
     gene            complement(1405..2802)
                     /locus_tag="PK134_06990"
     CDS             complement(1405..2802)
                     /locus_tag="PK134_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB38628.1"
                     /translation="MKNSRNNEPIKILFFLTVILSVISIMPKYLTGSKKINLYSDIIH
                     KENKKEKPQSLAIVNDSVFFKNDTVKQVLTDTAKSTATKINYKSGELDLTRFFEALHN
                     HENENGKIRIAWFGDSMIEGDLVTQDFRKLMQKIYGGSGVGFVPVTSPVAQFRQTIRQ
                     TFSDDWEVYSFMKKPKQNYKLGIGGYSFVSSKGSWVDYKASRFYPVYSEVKMICRQCP
                     PKSFILNEDTSLVFPVDSIGSFKEVELIRNNPIKEVKLTFKKPNCEVHGLYFDKGNGV
                     YVDNFSFRGNSGIPLSNIPFGELKSLGAKENYKLIILSYGLNVVAHDVKNYSWYASSF
                     QKTLNYIKEAFPDASILVMSVGDKSYRAEDGYETEPDIPIFVELQKRIAQRSDVAFYN
                     LYEAMGGYNSMVSWAEGNPQLANLDYTHPNFAGGRKIAGMLYDDIIKSYDRYSKTVET
                     MEVKRDTMIKPQAKQ"
BASE COUNT          896 a          545 c          403 g          967 t
ORIGIN      
        1 gtgttactgc aacaattcgt aaagttcctg ttcgttgcag aagtctaaat acgaacaaaa
       61 acaacaacaa ataaaaaaga aaaaacgaac tgttgaaaag caatggattt gctttatcat
      121 acaaaaactg atgtccaagc ttttcccaat ctattgtcat tgttcagata atggtttaag
      181 aagcgcctga agcaacatta atccctgcat ttcatatcct gaacggttca ggtgaattcg
      241 cctggcatca ctcattttat tttttgcaaa acgaagcata cttcccttgc cgcctgagca
      301 ctcatataaa tcaaaaaaag ctattttatt atccatgcaa tatttctgaa ggagattttt
      361 aatagcaatt acatttggat taggtacata cacaccacgt cttatacgtt tacatgattc
      421 cgttggtgaa gtaatcaata ttgacgctgt tggtgaaaca ttcctgagtt tagttataaa
      481 ggaatcaacg gtctgaataa atactgttcc atcaaaatac tttgcgaaag cctcgtttgt
      541 gccaaatgaa agaatataca ggtcagcatt cagttctttt acttgttcaa aaaacattgg
      601 ttgcccaaga aaacttttat actctgcacc atttacacca atattnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnatca ttcaaaagat ttaaaccgaa taatgtataa gtagtatcag
      721 aactgatgtt aattttacaa ttcgataaat aatcagaaaa tctgaactca ttaattcctg
      781 ttgcaagttt tattgggttt actgtttcaa ttattgctaa actatcactt gcagtcataa
      841 aagtactgtc aggtttataa aatacttgaa ctctgttaaa tctataatct ataccttcat
      901 tatttcgtaa gctaattcct acagatgggc tactgtcgtt ggttgttact gaaaatccgg
      961 tgatgccggt tgataaagaa tcttgtgtaa ttacatttct atttcctttc cagctattct
     1021 gagaaaaaga aacatagtca tgcgggccgt tagaacgcgc aagtttgtat ggaaatacaa
     1081 ttcccctacc ggcattacca tagcgttgtt gtagtcctct gcgaaccata cctgaaaaat
     1141 atccggcctg taaatgcgaa tcgccaatgt gcactatact aacttttttc ccgtcagctc
     1201 tactctgcaa caatttacta taaaaatttt tcaatgcttc tttctgttca atatcaattg
     1261 catcgggttt aagaaatttc aataaagaca ttttatattc aacaaatttc tcatattgca
     1321 acacaggcat tgtagtatcg gaatcactct gtgcatgaca ttttgtacaa tatattaaca
     1381 gagaaaaaat taaaattatt cttttcattg ttttgcctga ggcttaatca ttgtatctct
     1441 tttcacttcc atagtttcca ccgttttact atatctatca tagcttttaa taatatcatc
     1501 atacaacatt cctgcaattt ttcttccacc tgcaaaattg ggatgtgtat agtctaaatt
     1561 tgctaattgc ggattaccct ctgcccaact taccatactg ttataacctc ccattgcttc
     1621 atataaatta taaaaggcta catcacttct ttgtgcaatt cgtttttgca attcaacaaa
     1681 aatgggaata tctggctctg tttcgtagcc atcttctgca cgataacttt tatcacctac
     1741 actcatgact aagattgatg catcaggaaa tgcttcttta atgtagttta atgttttctg
     1801 aaatgatgaa gcataccaac tatagttttt cacatcatga gcaactacat ttaaaccata
     1861 gcttagaatt atcaacttgt aattttcttt tgcgccaaga gactttaatt caccaaaagg
     1921 aatattggaa agaggaattc cggaatttcc acgaaacgaa aaattatcta cataaacacc
     1981 gtttccttta tcgaaataaa gtccatgtac ttcacaattt ggttttttaa aggttaattt
     2041 aacttccttt ataggattgt tccttattaa ttcaacctct ttaaaactac caatactatc
     2101 aacagggaaa actaaagaag tgtcttcgtt caaaataaaa cttttaggtg gacactggcg
     2161 acaaatcatt tttacctctg aatataccgg gtagaatctg cttgctttat aatcaaccca
     2221 actacccttt gatgaaacaa aactgtatcc ccctattccc agtttataat tctgttttgg
     2281 ttttttcata aaactataaa cttcccagtc atcagaaaaa gtttgtctta ttgtttgcct
     2341 gaattgtgct acaggtgaag taacaggaac aaagccaaca ccgctgccac cataaatttt
     2401 ctgcatcagt tttctgaagt cctgtgtcac caaatcacct tctatcatag aatcaccaaa
     2461 ccatgcaatc ctgatttttc cattttcatt ttcatgattg tgcaatgctt caaagaacct
     2521 tgttaaatca agttcacctg acttataatt tatcttagtc gcagtagatt ttgctgtgtc
     2581 tgttaatacc tgcttaacag tatcattttt aaaaaataca gaatcgttta caattgccaa
     2641 actttgcggt ttttcctttt tattttcctt atgaataata tcgctataga gattaatttt
     2701 cttgctccca gtcaaatatt ttggcattat cgagattaca gataaaataa ctgtaagaaa
     2761 aaacagaatt ttaataggct cgttatttcg ggaatttttc acagcttatg attggcttta
     2821 ttgagcagta cgcgccttaa cc
//
LOCUS       DAORQT010000206         2832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7048_length_2832_cov_7.642420, whole genome shotgun sequence.
ACCESSION   DAORQT010000206 DAORQT010000000
VERSION     DAORQT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7048_length_2832_cov_7.642420"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..978)
                     /locus_tag="PK134_06995"
     CDS             complement(106..978)
                     /locus_tag="PK134_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38629.1"
                     /translation="MRFIISFVILLTHYISGYSQERRGPFVKTDLFTLDRDRVIDGGI
                     FTYRIAVDADEIIAFNSCNQPYVFLINNQGTILEEIKLPYEGCLRNMEFDEFDNLMMM
                     DNEETRIFKYVRKTKKIEVIPYNKPEDWYKSLNHFYRFFEIASIPTYYNNPEYYQDFY
                     KTRFHYSYNLWLNYQDGYIYQFAYNFIKKVGNRKTYLALKRSDLWFSDRLTNKCKPLL
                     VDLEKETAVYFDRSLTLYFEDFKNHTLQTWPLAEGHDEAVQLDYATNIKQKKVWGVST
                     FDNKHLTIAVWEIK"
     gene            1089..2282
                     /locus_tag="PK134_07000"
     CDS             1089..2282
                     /locus_tag="PK134_07000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38630.1"
                     /translation="MNTVVAIIFWISLLGLLHSYVLFPLLMRLLLMLKHKSKDDDDNN
                     LFKPMVSVVMAAYNEEKVIIQKLESVIKSNYPSDKLEIIVGSDCSNDSTDMLVKEFAH
                     NHPQVKFIRFNSRTGKPAIINQLVKQCKGEFLIITDANVMFDAEAISSMMHRFTVAEV
                     GLVDSNMVNIGLMKSGISSQEKQYIQTEVLTKYAEGEVWGSMMGPFGGCYAIRKSLFS
                     DIPSNFLVDDFYVCMKVLEQGYHSVSSLKAIVYEEASHNLWIEFRRKTRIATGNFQNL
                     AKFSKMIFKGRGIGFCFMSHKVLRWKGPVLMLLLYFSSIAAWWLSIDINPVLYLGVFA
                     AISLSLLLLLADRLLNLFQIHIGLLRLNTHFLVMNAAMLKGMFRYIKGVKSGVWQPTS
                     RTQNA"
     gene            2335..2724
                     /locus_tag="PK134_07005"
     CDS             2335..2724
                     /locus_tag="PK134_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38631.1"
                     /translation="MITTPLKIFIVDDDNMMLEMLRDRIGEHTPHTVLTFGTGEDCLK
                     HVAENPDVIILDYYLNSKNPDASNGMEILKAIKKYNPNIHVIFLSGQERYGVAMQTLQ
                     KGAEYYVIKDNEAFGKIEKILSGITVS"
BASE COUNT          909 a          441 c          543 g          939 t
ORIGIN      
        1 gttctaatgg catcatttgc actctggctg attaatctgg cagtgcctgc agcaattggg
       61 tcggtaatga ttttttattt aaatttcaga aaagaataac gctcattatt tgatttccca
      121 aacagcgata gtaagatgtt tattatcaaa agtacttaca ccccaaactt tcttttgttt
      181 gatattggta gcatagtcca actgcactgc ctcatcatgt ccttctgcca aaggccatgt
      241 ctgtaaagtg tggtttttaa aatcttcaaa atataaagtc agtgaacgat caaaataaac
      301 tgccgtttct ttttccagat caactaaaag cggtttgcat ttatttgtaa gtctgtcgct
      361 gaaccaaagg tcgctacgct ttaatgcaag ataggttttt ctgttaccga cttttttgat
      421 aaaattatag gcaaactgat agatgtagcc atcctgatag tttaaccaca ggttgtatga
      481 gtaatgaaag cgtgttttat aaaaatcctg atagtattcg ggattgttat agtaagtagg
      541 aattgaagca atttcaaaaa aacgataaaa atgattcaga cttttatacc agtcttcggg
      601 tttgttataa ggaatgactt ctattttttt tgtcttacga acatatttga agattcgtgt
      661 ttcttcattg tccatcatca tcaggttgtc aaactcatca aactccatgt tgcgcaaaca
      721 accttcgtaa ggaagtttaa tttcttcaag aatggttccc tgattgttaa ttaaaaaaac
      781 ataaggctgg ttacaagaat tgaatgcaat tatctcatcg gcatctactg caattctgta
      841 tgtgaaaatt ccaccgtcaa tcactctgtc gcggtcaagt gtaaaaaggt ctgtcttaac
      901 aaaggggcca cgtctttcct gactataacc tgaaatgtaa tgtgtaagaa gaataacgaa
      961 agaaataata aaccgcatgt aaacaaaaat taaggctgca aaagtcctct ttttattcat
     1021 ataaagccac aaaaagacag gtaaaatctt ctctatttga agttaaaatg ttgacctttg
     1081 cacaattgat gaatactgtt gttgccatta ttttctggat ttcgcttttg ggacttttac
     1141 attcttatgt actgtttcca ttactcatgc gtttactttt aatgttaaag cacaagtcta
     1201 aagatgatga tgacaataat ttatttaagc cgatggtcag cgttgtgatg gctgcttaca
     1261 atgaagagaa ggtcatcatt caaaagctcg aaagtgttat taaaagtaat tacccatcag
     1321 ataaattaga aataattgta ggctctgatt gttccaatga tagtactgat atgttggtga
     1381 aagagtttgc acataatcat ccacaagtaa agtttattcg attcaacagc agaacaggaa
     1441 aacctgcaat catcaatcaa ttggtaaaac agtgtaaggg tgagttttta attattactg
     1501 atgccaatgt aatgtttgat gctgaagcaa tttcatctat gatgcataga tttactgtag
     1561 cagaagttgg cttagttgat agcaacatgg tgaatattgg attgatgaaa agcggaattt
     1621 catctcagga gaaacagtac attcaaacag aagttttaac caaatatgcc gaaggtgaag
     1681 tatggggaag tatgatgggc cctttcggtg ggtgttatgc aataaggaaa agtttattca
     1741 gcgatattcc ttcaaacttt ctggttgatg atttctatgt ctgcatgaaa gtactggagc
     1801 agggttatca ttctgtaagt tccctaaaag ctattgtcta tgaggaggca tcgcataatc
     1861 tttggataga gttcaggaga aaaacgcgta tagccacagg aaattttcag aatctggcaa
     1921 agtttagcaa gatgattttt aagggcagag gtattggatt ttgtttcatg tctcataaag
     1981 ttttgagatg gaaaggccct gtgttgatgt tgcttttata tttctcttct attgcggcct
     2041 ggtggttgtc tattgatata aatcctgttt tgtatctggg agtttttgca gccatatcat
     2101 taagtttact tttgttgctg gcagacaggc tacttaattt gtttcaaata catataggat
     2161 tgctcagatt aaatacacat ttcttagtca tgaatgctgc tatgttgaag ggtatgttca
     2221 gatacattaa aggagttaaa agcggtgttt ggcaacctac aagccggact cagaatgctt
     2281 aattggatat tacaaaaaaa ttgtaggttt gtattccatt tgacactaaa caaaatgata
     2341 acaacacctt taaaaatatt tattgttgat gatgataata tgatgctgga aatgcttcgt
     2401 gacagaattg gtgagcatac accacatacg gttttaactt tcggcacagg cgaagattgt
     2461 ttgaagcatg tagcagagaa tcccgatgtt attattcttg attattatct gaattcaaaa
     2521 aatcctgatg catctaatgg tatggaaata ttgaaagcta tcaagaaata caatccaaac
     2581 attcatgtaa tttttctttc gggacaggaa cgctatggtg tggctatgca aaccttacaa
     2641 aaaggagcag agtattatgt cattaaagac aatgaagctt ttggtaaaat tgaaaaaata
     2701 ttaagcggca ttacagtttc ataaatcaac tctaaaaagt tgatactgaa attttttatt
     2761 caccgcttag ttatcagttg tcgtcaatac accactcaga ataagaagta ggagtttctt
     2821 ctaattttaa at
//
LOCUS       DAORQT010000207         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7131_length_2813_cov_19.116751, whole genome shotgun sequence.
ACCESSION   DAORQT010000207 DAORQT010000000
VERSION     DAORQT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_2813_cov_19.116751"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="tgt"
                     /locus_tag="PK134_07010"
     CDS             complement(<1..1109)
                     /gene="tgt"
                     /locus_tag="PK134_07010"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRB38632.1"
                     /translation="MHFSIEKYDKESKARSGRIETAHGIIETPIFMPVGTAGTVKSIN
                     QRDLKQDVKAQIILGNTYHLNLRPGIEVLHQAGGLHKFMNWSLPILTDSGGYQVFSLS
                     ETRKITEEGVKFNSHIDGSKHFISPEVSMDIQRFIGADIIMAFDECTPYPCEHRTAKK
                     SMEMTHRWLQRCISHLEKSPAKYGYEQTLFPIVQGSVFKDLRTESSKVIADAGAKGNA
                     IGGLSVGEPAEIMYEITDVVTDILPVEKPRYLMGVGTPANILESIALGIDMFDCVMPT
                     RNARNGMLFTAEGIINIRNEKWKNDFSPIDESGNCFADTDYSKAYLRHLFISKEMLAA
                     QLATLHNLSFYLWLVTEARKRINDGSFLSWKNLMV"
     gene            1368..2369
                     /locus_tag="PK134_07015"
     CDS             1368..2369
                     /locus_tag="PK134_07015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38633.1"
                     /translation="MSEIEPPVSIIICARNELKNLKAYLQQVLEQDYPRFQVIVVNDC
                     SWDESEKYLEELSATYTNLKVVSIKEQERYQHGKKFALTIGIKSALYDHLLLIDADCY
                     PASNQWIRLMMQSYNAKTRIVIGYGAYIKTSSLLNRWVRFDTAFNAMQYLSFALAGNP
                     YMGVGRNLSYQRSLFFENKGFARHQHIMSGDDDLFINDTATTENLSVQINPDSFTYSQ
                     PKTDFIDWFRQKKRHISASRLYKFKHKMLLGIYYFSHISFLVLFIALLIIQFKIEYVV
                     TAFSLRLVFQMIIFGSCLKKLKEGDLIPLIPIFDIWIAVVYPLIAISNRLIKNKTWK"
     gene            2360..>2813
                     /locus_tag="PK134_07020"
     CDS             2360..>2813
                     /locus_tag="PK134_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB38634.1"
                     /translation="MEVKSPKTAKAAQDKTEVNPNLSDKAVVDYQLVREAVDHGNQKA
                     YAELMSRYKDSIYFMLLKMVNNRDDADDLTIEAFGKAFKNIHQYTPDYAFSTWLFKIA
                     TNNCIDYIRKKRKLMLSIDKGYENENGEDVALEIKSDTIGPEEKLMKKQ"
BASE COUNT          897 a          508 c          484 g          924 t
ORIGIN      
        1 ttcaccatta aatttttcca tgaaagaaaa cttccatcat taattctttt tcttgcttct
       61 gtaactaacc aaagatagaa agacaggttg tgaagtgttg ctaattgtgc ggcaagcatt
      121 tctttgctga taaataaatg ccttaaataa gctttggaat aatctgtatc tgcaaaacaa
      181 tttcctgact catctatggg cgaaaaatca tttttccatt tttcgtttct gatattaatg
      241 ataccctcag ccgtaaaaag cataccgtta cgtgcatttc tggttggcat aacacaatca
      301 aacatatcta tacctaaagc aatactttca agaatatttg ctggagttcc tacacccatc
      361 agatagcgcg gtttttcaac aggtaaaata tctgtaacca catcagtaat ttcatacata
      421 atttcagcgg gctcgccaac ggatagacca ccaattgcat tgccttttgc accggcatca
      481 gcaattactt ttgaactttc tgtgcgcagg tctttaaaaa cagatccttg aacaatagga
      541 aagagtgttt gttcgtaacc atactttgcc ggagattttt caagatgaga tatgcatcgt
      601 tgtagccagc gatgtgtcat ttccattgac ttttttgccg ttctatgctc gcatggatag
      661 ggcgtacatt catcaaatgc catgattata tcagcaccaa taaaacgctg tatgtccata
      721 ctcacttcag gagaaatgaa atgtttgctt ccatcaatat gtgagttgaa tttaacccct
      781 tcttctgtta tctttcttgt ttcgcttaaa gaaaaaacct ggtatccgcc actatccgtt
      841 aatattggta gcgaccaatt cataaactta tgcagtccac ctgcctgatg caaaacttca
      901 atgccgggac gtaaattcag atgataggtg ttgccaagaa taatctgtgc tttaacatcc
      961 tgttttaagt cgcgctgatt aatgcttttg actgttcctg ctgtgccaac aggcataaaa
     1021 attggagttt caataatgcc atgagcggtt tctattctgc ccgagcgggc tttactttct
     1081 ttatcatact tttcaataga aaaatgcata gattcagtaa tttttgcaaa aagtgttgtt
     1141 aatattttta caaagataga aaagccttct gcaatgactc ttcatacatt tgcgcgctta
     1201 ccaacaccaa agttattttg atacccatac ctataccaga ttttataagc attgaaaata
     1261 tttcattggt acttttttat ttgcttgtag tatttattac catacaacta atctattatt
     1321 tatttgtttt tgtgcgtttg tctctgcata aaacagtaaa cgccccagtg tcagaaattg
     1381 agccgcctgt ttcaataata atttgtgcca gaaatgaact gaaaaaccta aaggcatatc
     1441 ttcaacaagt attggagcaa gactatcctc gatttcaggt tatagttgtg aatgattgtt
     1501 cgtgggatga atcagaaaag tatcttgaag aactatctgc aacctataca aatcttaaag
     1561 tagtttcaat caaagaacag gaacgttatc agcatggaaa aaagtttgcc ttgacaattg
     1621 gaattaagtc tgccttatat gaccatctgt tgttgattga tgcagattgt tatccggcaa
     1681 gcaatcagtg gatacgactg atgatgcaaa gttataatgc aaaaacaaga atagtaattg
     1741 gttatggtgc ctatattaaa acttcttcac tattaaaccg ttgggttaga tttgacactg
     1801 catttaatgc aatgcaatat ctttcatttg cacttgcagg aaacccttac atgggtgttg
     1861 gaagaaattt atcatatcag cgttcactgt ttttcgagaa taaaggcttt gcccgacatc
     1921 aacatattat gtctggcgat gatgatcttt ttataaatga tacagccact acagagaatc
     1981 tttctgttca gataaacccc gattctttta cttattcaca gccaaaaacc gactttattg
     2041 actggttccg tcagaagaaa aggcatataa gtgcttctcg cttatataag tttaaacata
     2101 aaatgctttt aggcatttac tatttttcac atatttcatt ccttgtttta tttatagcat
     2161 tgttaataat tcagtttaaa attgaatatg tagttactgc ctttagttta cgtttggttt
     2221 ttcaaatgat tatttttggc tcatgcttaa aaaaattaaa ggaaggcgat ctgattccgt
     2281 tgataccgat attcgatata tggatagcag ttgtttatcc tttaattgcc atctccaatc
     2341 gtttaataaa gaataaaaca tggaagtaaa aagtcccaaa actgcaaagg cagcacaaga
     2401 taaaacagaa gttaatccta acttaagtga taaagcagtt gtagattatc agctggttcg
     2461 tgaagcagta gatcatggca atcaaaaagc ttatgctgaa ctaatgtcgc gctacaagga
     2521 ttctatctat tttatgctac ttaaaatggt caataatcgt gacgatgctg atgatttgac
     2581 aattgaagct tttggcaaag catttaagaa tattcatcaa tatacacctg actatgcttt
     2641 cagcacctgg ctatttaaaa tagcaaccaa caattgcatt gattatatcc gcaaaaagcg
     2701 aaagttaatg ttgagtattg ataaaggata tgaaaacgaa aatggcgaag atgttgcttt
     2761 ggaaataaaa tctgacacca taggtcctga agaaaagctg atgaaaaagc aaa
//
LOCUS       DAORQT010000208         2807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7156_length_2807_cov_8.081759, whole genome shotgun sequence.
ACCESSION   DAORQT010000208 DAORQT010000000
VERSION     DAORQT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7156_length_2807_cov_8.081759"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK134_07025"
     CDS             complement(<1..287)
                     /locus_tag="PK134_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRB38635.1"
                     /translation="MINDFFKLFFPDNCPGCGHSFLRGEKYVCLQCLYGLHKTQYHND
                     PENPIAKNFWGKTDFVAVTAVYFFQKGQVLQRLLHQLKYNGCKEIGIMLGR"
     gene            494..1429
                     /locus_tag="PK134_07030"
     CDS             494..1429
                     /locus_tag="PK134_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38636.1"
                     /translation="MEESVNEQTKADNREQNSRRALVFIVITILLGVNGLLLYQFFDK
                     KSHLEEVTRTLDNTSAERDALSAELQQVKAEFEKINQENSSLQAQLLSKDEEIKSKMA
                     QIRRMIESGDASQLKAAKAELERLRSMNQVYVAQLDSMRNANDALASQNESLNDMITV
                     EKNKSTALALENSTLTKRVAAASVLRTTSIKASGVKYKSSGKELETNKASSAQKIKTC
                     FTILENTVATSGSKDIYIRILSPDGVVMTNNTETFNVAGQSSLYSLKESFDYENREMN
                     MCVYWEKGTAFSPGKYTVEAYCEGSLIASTPVVLK"
     gene            complement(1435..>2807)
                     /locus_tag="PK134_07035"
     CDS             complement(1435..>2807)
                     /locus_tag="PK134_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38637.1"
                     /translation="DSLMLNSFGHDKAGVYLASADTGRWTYKTEVSRRWDYLPSTNEL
                     KLSTTADQADADIAFIAGRGKKITLGSTYRTIQVNRKEITSQKKDESFLGKLEIALQF
                     LRGGITSNTYYEVGAGQEQRQAYSFIKVAAGAGVFEWNDYNGNGIAELNEFEVAAFQD
                     RAEYIKIFTPTNDYVRIQTNQLSEVISVNPSAFIKNKQGKTRLINRFALQSAIRFDNK
                     LLSGKWADGLNPFGYNINDTSLLITNSTLRNTLFFNRSSSVFAADFNYDNISNKSLLA
                     NGTEGRNLTSWLLNTRTYFKRVYGLTTALKYETKQNSSVFTNRNYEIAGYSIEPRFSV
                     QPGTVFRTTLIYKYQDKQNVIELANEHATITTIGAECKYSSFKNGVASLGMNIISIGY
                     NADVNSPLAFEMLEGLKKGRNYTWEISLQKNLTGNLQINIGYNGRKPQGDKIIHTANV
                     QARAIF"
BASE COUNT          854 a          548 c          515 g          890 t
ORIGIN      
        1 tgtctgccta acatgatgcc tatttctttg catccattgt actttagctg atgtaaaagt
       61 cgttgcagca cctgaccttt ctggaaaaaa taaactgccg tgacagctac aaaatctgtc
      121 ttgccccaga aatttttcgc tataggattt tccgggtcgt tatgatactg tgttttatga
      181 agtccgtaca aacattgcag acacacatat ttttcacctc tcaaaaaact atgcccacag
      241 cccgggcagt tgtcggggaa gaacaattta aaaaaatcat ttatcacaag tggcggtttt
      301 aaatcaagaa gtaaatatat gcttttgttt aaaaaaatgt aaggtttacc gaataaaaaa
      361 agcaaattaa gcggtggcca aggaaactca aaattgtcat tttacgtaga aacagagaat
      421 atttcatcct ttttacttgc ttgtaaatac taattacatt attttgcacc gtttaacaac
      481 ccatttcaga tagatggaag aatcagtgaa tgaacaaacc aaagcagata accgtgagca
      541 aaactcacgc agagcactcg tatttattgt aatcaccata ctattggggg ttaacggatt
      601 gttgctatat cagttttttg ataaaaaaag tcatttggaa gaagttacaa gaactcttga
      661 caatacttct gctgaacgcg atgccttgtc agcagagttg caacaggtaa aagccgaatt
      721 tgaaaaaata aatcaggaga actccagctt gcaggctcag ttgcttagta aggatgaaga
      781 gattaagagc aaaatggcgc aaatcagaag gatgattgag tcgggtgatg cttcacagtt
      841 aaaggctgca aaagcagagt tagagcgact gagatcaatg aatcaggtat atgttgcaca
      901 gttagactcc atgcgaaatg ccaatgatgc attagcttca caaaatgagt cgttgaatga
      961 tatgattaca gtagaaaaaa ataaatctac tgcattagca cttgaaaatt caacattaac
     1021 aaaaagagtt gctgctgcat cagtactaag gactacttca attaaagcat ccggagtaaa
     1081 atataagagc agtggcaagg agttagaaac caacaaagca tcatcggcac aaaaaatcaa
     1141 gacctgcttt acaattcttg aaaacactgt tgccacatca ggctctaaag atatttacat
     1201 cagaatactt agccctgatg gtgtggtgat gaccaacaac acagaaacat tcaacgttgc
     1261 cggacagtct tcactttatt cattaaaaga atcttttgac tacgaaaacc gcgaaatgaa
     1321 tatgtgtgtt tattgggaaa aaggaactgc ctttagcccc ggaaaatata ctgttgaagc
     1381 gtattgtgag ggtagcctga ttgcatctac tcctgttgtt ctgaaataat tttcttagaa
     1441 aattgctctt gcctgcacat tggcagtgtg aataatttta tcaccctgcg gcttgcgtcc
     1501 gttatatcca atattgattt gcaggttgcc tgtaagattt ttctgaaggc ttatttccca
     1561 tgtatagttt cttcctttct taagtccctc aagcatttcg aaagctaatg gtgaattaac
     1621 atctgcatta tatccaatac tgataatatt cattcctaaa gatgctacac catttttaaa
     1681 gctgctgtat ttacactctg cacctatggt tgtgattgtt gcatgttcat ttgccaattc
     1741 aattacattt tgtttgtcct gatacttgta gatgagtgta gttctgaaaa ctgttcccgg
     1801 ctgcacgcta aaacgcggct caatggaata gcctgcaatt tcataattcc tgtttgtaaa
     1861 aaccgaactg ttttgtttgg tttcatattt caacgctgtt gttagaccat acactctttt
     1921 aaaatatgtt cttgtattta acagccatga tgttaaattt cttccttcag tgccattggc
     1981 aagcaaactt ttattgctga tgttatcata attaaaatcg gcagcaaaca ctgatgagct
     2041 gcgattaaaa aataatgtgt ttcgcaaagt agagttggtg atgagcaatg atgtgtcgtt
     2101 aatattgtaa ccaaaaggat tcagtccgtc tgcccatttt ccggaaagca gtttgttatc
     2161 aaatcgtatt gccgattgca aagcaaacct gtttatcagt ctggtcttgc cctgcttgtt
     2221 ttttatgaat gccgatgggt tgacactgat tacttcactt agctgattgg tttgaattct
     2281 gacataatcg tttgttggcg taaatatttt tatatactct gctctgtcct gaaatgcagc
     2341 tacttcaaat tcattaagtt cggcaatgcc gttaccgtta tagtcgttcc actcaaaaac
     2401 acccgcccct gctgcaactt taataaatga gtaagcctgt ctttgttcct gccctgcccc
     2461 aacctcataa taggtattgc ttgttattcc acctcttaaa aactgtaatg ctatttctaa
     2521 ttttcccaga aagctttcat cctttttctg acttgtaatt tcttttctgt ttacctgaat
     2581 tgttctgtat gtactgccta aagtaatttt ctttcctctt ccagcaataa atgcaatatc
     2641 tgcatcggcc tgatcggcag tggttgaaag tttaagctca ttagtacttg gcagataatc
     2701 ccatctgcga gatacctctg ttttataagt ccacctgccg gtatctgcag atgctaaata
     2761 aacacccgcc ttatcatgac caaaactgtt tagcatcagg ctatcac
//
LOCUS       DAORQT010000209         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7173_length_2802_cov_4.588278, whole genome shotgun sequence.
ACCESSION   DAORQT010000209 DAORQT010000000
VERSION     DAORQT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7173_length_2802_cov_4.588278"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PK134_07040"
     CDS             <1..530
                     /locus_tag="PK134_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRB38638.1"
                     /translation="LEINNLQKVYMKGQIEMAAENKKNIYPDANFTLRITYGKVEGYE
                     PRDGIAYKYYTTLDGVAEKYDSTNYDFNVPEKLLELHKKKDYGRYATDGKLVPCFLAS
                     NHTTGGNSGSPVLNGQGQLIGINFDRVWEGTMSDIMFDPAKCRNITLDIRYVLFIVDK
                     FAGAKWLTEEMNIVQ"
     gene            complement(534..2291)
                     /locus_tag="PK134_07045"
     CDS             complement(534..2291)
                     /locus_tag="PK134_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38639.1"
                     /translation="MKRILLSLAFTITGFLAFAQCSIDSTQTDPGIYPDTLLPATVNQ
                     PYTQDITFVMITDTMGITITNFNIANITGLPVGMTWQCNNFANGCNYDPSQSIYGCVN
                     LSGTPLIAGVYNATVTVIATLQVLGDQTISYTLPFTVLPDTVSNPGFSMTNSSGCLPL
                     SVTFVTNNPGQASYLWDFGNGIQSTLENPPIQTYNAPGDYVVTQTVTANGSPQYFLTD
                     ITVASIPDNYGAPVDVPDMYFYLYDSQGNQIYDSHPSVSNTNAPLSWTLPNIPLQNNN
                     YTLHVWDEDGGLFGADDDLGSVTFPGWSASGTATGTLSGVSGSLVVNYSIMQVPVATA
                     IHTDTVHVYAVPDTPMVSANIPLILCTGDTVVLTCNDTLQVQWYESGNLLIGETNSDL
                     VVTASGNFSAVVTNAHGCTATSVTISVQVNPNPPKPTFFVNGNTLNCVLTGYMLQWYF
                     NGSALTGETALQLTATQQGTYSVIATDSISGCSSVSDPLVFTPVGIDQLQSVISAILF
                     PNPADKSVVVQLTSGRNVFTTVQLLDISGWLLQQKDFMLQQGSTQLPFDLTDTSKGLY
                     LIKITQKNASKTLRLVISR"
     gene            complement(2381..>2802)
                     /locus_tag="PK134_07050"
     CDS             complement(2381..>2802)
                     /locus_tag="PK134_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRB38640.1"
                     /translation="KAGEKGKVFGIDLKPVEVKLPNVFTVQDNIETFDLQTLFDRYGF
                     NDKFDVVMSDMAPNTTGSRFTDQARSFDLSMLALQTAKRFLRPGGHFVCKIFDGEDAM
                     TFRDEVKSCFGECFVLRPKSTRNSSKEIFVTGKYFKT"
BASE COUNT          914 a          545 c          540 g          803 t
ORIGIN      
        1 ccttagaaat aaataatctg caaaaagtat atatgaaagg tcaaatagaa atggcggctg
       61 agaataaaaa gaacatctat ccggatgcaa actttacact tcgcattacc tatggcaagg
      121 ttgaaggtta tgagccacgt gatggaattg cttacaaata ctatacaact cttgatggag
      181 ttgcagaaaa gtacgattca acaaactatg attttaatgt accagaaaaa cttcttgaac
      241 ttcataaaaa gaaagactat ggccgttatg ctacagatgg aaaattagtc ccctgttttc
      301 tggcatccaa tcacacaaca ggaggtaatt caggaagtcc tgttctcaat ggacaaggtc
      361 agttaatcgg aattaacttc gatcgcgttt gggaaggtac aatgagcgac attatgtttg
      421 accctgcaaa atgcagaaac atcacattag atattcgcta tgttttgttt atcgttgaca
      481 aatttgcagg tgccaaatgg ctaaccgaag aaatgaatat tgtgcagtaa cactcaacgg
      541 ctaatcacta gccgcaaagt tttagaggca tttttctgtg ttattttaat cagataaaga
      601 cctttacttg tatcagttaa atcgaatggc agttgagtag agccttgttg cagcataaaa
      661 tctttttgtt gcaacagcca tccgctaata tctaaaagct gaactgttgt aaaaacattt
      721 ctacctgatg taagttgcac tacaacactt ttatctgcag ggtttggaaa taagattgct
      781 gaaatgacag attgaagttg atctattcca accggagtaa acactaatgg atctgataca
      841 gagctgcatc ctgaaatgct atcagtagca attacagaat aagtgccttg ttgagttgct
      901 gttaattgca atgcagtttc accggtcaat gctgaaccat taaaatacca ctgtaacata
      961 taaccggtta gcacgcagtt taaagtatta ccattgacaa aaaacgttgg ttttggtgga
     1021 ttaggattca cctgaacact aatagttact gaggttgctg tgcaaccatg agcatttgta
     1081 acaacagcag aaaaattacc tgatgcagtt acaactaagt ctgaattggt ctccccaatc
     1141 agcaggttgc cactctcata ccattgaacc tgcaatgtat cattacaagt caacacaaca
     1201 gtatcgcctg tacacaaaat caatggaatg ttagcactta ccatcggtgt atcaggaact
     1261 gcgtaaacat gcacagtgtc tgtatgaata gctgtagcaa caggtacctg cattattgaa
     1321 taattcacta ctaacgaacc ggaaacacca gacaatgtac ctgtggctgt tccactggca
     1381 ctccatcccg gaaaggttac ggagcccaaa tcatcatctg caccaaacaa tccaccatct
     1441 tcatcccata catgcaaggt gtaattgtta ttttgtaaag gtatgttggg caatgtccaa
     1501 gataagggtg catttgtatt tgaaacagaa ggatggctat cgtaaatctg attgccttga
     1561 gaatcgtaca gataaaaata catatcagga acatcaaccg gtgcaccata attgtcgggg
     1621 atactggcta cggtaatatc agtcagaaaa tattgtggtg aaccattggc tgtaacagtc
     1681 tgtgttacta cgtaatcgcc aggtgcgtta tatgtctgta tcggtggatt ttccagtgta
     1741 ctctgaatac catttccaaa atcccacaaa tatgacgcct gaccgggatt attggttaca
     1801 aacgttacac tcaatggcaa acacccactg ctattggtca ttgaaaagcc cggattgcta
     1861 acagtgtcag gtaaaactgt aaatggtaat gtgtagctaa tagtttgatc acctaaaact
     1921 tgtaatgtgg caatgacggt gacagtagca ttgtaaacac cggcaattaa tggtgttccg
     1981 cttaaattaa cgcaaccata aatagactga gaaggatcat aattacatcc attagcaaag
     2041 ttgttgcatt gccatgtcat acctaccggt agtccggtaa tatttgcgat gttaaaattt
     2101 gttatggtta tgcccatggt atcagtaatc atcacaaagg tgatgtcctg agtgtaaggt
     2161 tgattcacag tggcaggcaa taaagtgtcg ggataaattc cgggatcagt ctgtgtactg
     2221 tcaatgctgc attgagcaaa tgcaaggaaa cctgtaatgg taaaagctaa actaagtaaa
     2281 attctcttca tatccatttc ttttagttga gtaaaaatag ctaatgattt gaagtgcaaa
     2341 aacaaatttg cagaaactta ttaatacact tactaaaata tcaggtttta aaatactttc
     2401 cagtcacaaa tatttctttt gagctgttgc gtgtgctttt aggtcgtaaa acaaaacatt
     2461 caccaaaaca tgattttact tcatcgcgaa aggtcattgc atcttcgcca tcaaatattt
     2521 tacaaacaaa atgacctccc ggccgcagaa aacgttttgc tgtttgaagg gcaagcatgc
     2581 taaggtcaaa agaacgagcc tgatcggtaa aacgacttcc tgtagtattt ggagccatat
     2641 cactcattac tacatcaaac ttatcattaa agccatagcg gtcaaacaag gtttgcagat
     2701 caaaagtttc tatgttatcc tgaacagtaa acacattggg caatttcact tctaccggct
     2761 tcaggtctat accaaaaact ttacccttct cccccgcttt tt
//
LOCUS       DAORQT010000210         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7199_length_2793_cov_10.395179, whole genome shotgun sequence.
ACCESSION   DAORQT010000210 DAORQT010000000
VERSION     DAORQT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_2793_cov_10.395179"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2252
                     /gene="katG"
                     /locus_tag="PK134_07055"
     CDS             69..2252
                     /gene="katG"
                     /locus_tag="PK134_07055"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRB38641.1"
                     /translation="MDNTKSGKCPFHHGANTEASTGVMNWWPKALNLDILHQHDTKTN
                     PNGEKFNYAEEFKKLDLEALKSDLKKLMTESQEWWPADHGSYSGMFARTAWHLAGTYR
                     KADGRGGANTGNQRFAPLNSWPDNVNTDKGRRLLWPIKKKYGNKISWGDLIVLAGNMA
                     YEVAGLKTFGFGGGREDIWHPEKDIYWGSEKQWLAPTGSEGTRYSGERDLENPLAAVM
                     MGLIYVNPEGVDGKPDPLKTAQDIRVTFSRMAMSDEETVALTAGGHTVGKAHGNGKVE
                     VLGGVPEAAEIQHQGLGWFNPDGSGNKDKTMASGIEGAWTTDPTKWDNGFFDLLLNYD
                     WALTKSPAGAWQYEPTNMPEEKKPVDAFDPKVRRNPMMTDADMALKMDPEFRKISERF
                     YNDFNYFSETFARAWFKLTHRDMGPKSRYLGADVPKEDLIWQDPIPAVSYTLSDAEAD
                     EIKTKILNSGLSRTELINTAWDSARTYRGSDFRGGANGARIRLEPMKNWEGNEPKRLQ
                     KVLDKLTEIQKGLSKKVSIADLIVLGGSAAVEQAAKDAGMSVKVPFAAGRGDATLEQT
                     DVDSFSDLEPLHDGYRNYQQKNYAVSSEELLLDRTQLMGLTAPEMVVLVGGMRVLGTN
                     FGGSKHGVFTEREGVLTNDFFVNLTDMKYEWKPVSENLFNIVDRKTGATKYTATRVDL
                     VFGSNSILRSYSEVYAQDDNKEKFVKDFVKAWVKVMNADRYDLKK"
     gene            complement(2334..>2793)
                     /locus_tag="PK134_07060"
     CDS             complement(2334..>2793)
                     /locus_tag="PK134_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB38642.1"
                     /translation="DQNGVSLERIWFNKGTQDSTNWHSAAEAVGYATPGYKNSQSLNE
                     QLQENNFTITPEIFSPDNDGYNDVVTFNINMNEPGYMLNAKIYNEAGQLVRNLVKSKL
                     LAPEDSFMWDGITDKNDKASVGIYIVYAEAFNPEGKTKKYKKAFVVASKL"
BASE COUNT          911 a          457 c          618 g          807 t
ORIGIN      
        1 ttcaaagtgc aagacattaa attacagatt acaggaactt tcaaataaaa aaataaaaca
       61 cacatattat ggacaacaca aaaagcggca aatgcccgtt tcatcatgga gccaacaccg
      121 aagcaagtac aggtgtcatg aactggtggc ccaaagcatt gaacttagat atcctgcatc
      181 agcatgatac aaagactaat cctaatggag aaaaatttaa ttacgcagaa gaatttaaaa
      241 aattagattt agaagcttta aaatctgacc tgaaaaaatt gatgactgaa agtcaggaat
      301 ggtggcctgc agatcatgga agctattcgg gtatgtttgc gcgtactgcc tggcacttag
      361 caggcactta cagaaaagct gatggtcgtg gtggtgcaaa caccggcaat cagcgttttg
      421 ctcctctcaa ttcatggcct gacaatgtga atacagataa gggaagaaga ttgctatggc
      481 ctatcaagaa aaaatatggt aacaaaattt cctggggcga tcttattgtt ttagcaggta
      541 atatggctta tgaagttgca ggactaaaaa catttggttt tggtggaggt agagaagata
      601 tatggcatcc tgaaaaagat atttactggg gatcagaaaa acaatggctt gcaccaactg
      661 gtagtgaagg cacacgttat tccggtgaaa gagacttaga aaatcctttg gcagctgtaa
      721 tgatgggatt gatttatgtt aaccctgaag gtgttgatgg aaaaccggat ccattaaaaa
      781 cagcgcaaga cattcgcgtt actttctcac gaatggcaat gagtgatgaa gagactgttg
      841 cacttactgc cggtggacat actgtaggca aagcacatgg taacggaaaa gtagaagtgt
      901 taggtggtgt tcctgaagct gcagaaatac aacatcaggg attgggttgg tttaatcctg
      961 atggctctgg caataaagat aaaactatgg caagtggtat tgaaggagca tggactacag
     1021 acccaacaaa gtgggataat ggattttttg atttgttatt gaactacgat tgggcattga
     1081 caaaaagccc tgccggtgca tggcagtatg agccaacaaa tatgcctgaa gaaaagaaac
     1141 ctgttgatgc atttgatcca aaagttcgca gaaatcctat gatgactgat gctgatatgg
     1201 cattaaagat ggatcctgaa ttcagaaaga tatcagagag attttataat gacttcaatt
     1261 atttttctga aacatttgcc cgtgcttggt ttaaacttac gcatagagat atgggaccaa
     1321 agtcgcgtta ccttggtgcc gatgtgccta aagaagattt gatttggcaa gatcctattc
     1381 ctgcagttag ttacacattg tcagatgctg aagcagatga aataaaaact aaaattttaa
     1441 actcaggttt aagcagaaca gaattgatta atactgcatg ggattctgca agaacatatc
     1501 gtggatcaga tttcagaggc ggtgccaatg gtgcaagaat tcgtcttgag ccaatgaaaa
     1561 attgggaagg caatgagccg aaacgcttac aaaaagtgtt ggataaactc acagaaattc
     1621 aaaaaggatt aagcaagaaa gtaagtattg cagatttaat tgtgttgggt ggtagtgctg
     1681 ctgttgagca ggctgcaaaa gatgcaggca tgtcagtaaa agttcctttt gctgccggca
     1741 gaggtgatgc aacattggag cagactgatg tagattcttt ttctgatttg gaacctttac
     1801 atgatggata cagaaattat cagcaaaaaa actatgctgt ttcttcagag gaattgttgt
     1861 tagacagaac acagttaatg gggctcactg cacctgaaat ggttgtgctt gttggaggaa
     1921 tgagagttct gggaaccaac ttcggtggaa gcaaacatgg tgtgtttaca gagagagaag
     1981 gtgttttaac taatgacttc tttgtaaacc ttactgatat gaaatatgag tggaagcctg
     2041 tttctgaaaa tctgttcaat attgttgata gaaaaacagg agctacaaaa tacacagcta
     2101 cacgagtaga tttggttttc ggttccaatt caattttaag gtcatactca gaagtgtatg
     2161 cacaggatga taacaaagaa aaatttgtga aagattttgt gaaagcctgg gtaaaggtga
     2221 tgaatgctga cagatatgat ttgaaaaaat aatattgttt agtactccaa aaatgcgagc
     2281 agcctctgaa aagaggctgc tcgcatttta tatcatctgc ttaactgtaa ccactaaagt
     2341 tttgaagcaa ccacaaaagc ttttttatac tttttagttt ttccttcggg attaaatgct
     2401 tcggcataaa caatataaat tcctacagag gctttatcat ttttatcggt tatgccatcc
     2461 cacataaaac tatcttccgg tgctaatagt tttgatttta ccaagttgcg aaccaattgt
     2521 cctgcttcat tataaatttt tgcattcagc atataacccg gttcattcat gttgatgtta
     2581 aatgttacta catcattata tccatcgtta tctggcgaaa aaatttctgg ggttattgta
     2641 aaattatttt cctgtaattg ttcgtttaaa ctttgtgagt ttttatagcc cggtgttgca
     2701 tagccaacag cttctgctgc cgaatgccag ttagtagaat cctgtgtgcc tttattaaac
     2761 catattcttt ctaatgaaac accattctga tcg
//
LOCUS       DAORQT010000211         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7235_length_2783_cov_5.710777, whole genome shotgun sequence.
ACCESSION   DAORQT010000211 DAORQT010000000
VERSION     DAORQT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_2783_cov_5.710777"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2048
                     /locus_tag="PK134_07065"
     CDS             138..2048
                     /locus_tag="PK134_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB38643.1"
                     /translation="MSKKTIFNLLLCFCVVSIHAQNLKRCPQTTSKEAAKKTEEAEKL
                     LKSVKNYEEGVELCNEAIEADSTFAKPYLLMGDAAYRKKDFKLMAKAYKGLMNVCPDA
                     SEKAIYRLATYYFDTKKYEEAIPVYTTFLELSPSDTNAADDAEVKLFRAKAFLHPVDF
                     KPVLVKGLSTPDPEYLPIISADNELCFFTRRFEYQSKNMLTSTSVEKFMLSHNLNGKW
                     DTGTPMDLPFNKQNTGNEGGPTISLDNLHLFFTVNVKGNFDIYTSDLTPKGWSEPRSI
                     GKANDPKFWDSQPSLSPDNKTLYFASFRDSVLQTSDIYVTKKNNGEWNSANPISGPIN
                     TSRNEKAPFMHPDGKTLYFSSDGHPGMGGYDIYYSKKDANGNWGKPVNLGYPINTDAD
                     EVGFFVSTDGTKGFFASNKFGTGGYDIYSFDMPVEARPEKMVLFKGQLKNDDNEVPDS
                     ARIEIRNISSKQVEQIDYDAYSGKYAGVLQANSEYLLTIQKDDYAFTSKLIDAAKVDS
                     PAVTVELDLRKIKEGEAYSLNDILFETDSYQITDKSKAVIQDFAGFLKSNPKLKVAIY
                     GHTDNSGKAEDNLLLSKNRAKAVYDYLIALGIEATRLSHNGFGQTKPVAANTTEKGKS
                     LNRRTEFVVLKK"
     gene            2053..2547
                     /gene="idi"
                     /locus_tag="PK134_07070"
     CDS             2053..2547
                     /gene="idi"
                     /locus_tag="PK134_07070"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRB38644.1"
                     /translation="MEFVILVDEQDSEVGEAEKMQTHKLGLLHRAFSVLIYNSNGEML
                     LQQRAETKYHSGGLWSNACCGHPRPGENIEDAAKRRLKEELGFNCDLKFSFKFLYKVS
                     FENSLKEHELDHVFTGSYESEVLPDKSEVQAFRWITMNNLKNEITENPDKFSVWFLKI
                     ISEL"
BASE COUNT          950 a          420 c          556 g          857 t
ORIGIN      
        1 tgttgacaaa ggaaaacaag tagattattt tgtttatccc ggacatccac ataatgttcg
       61 cggtaaagat cgtgtgcatc taatgaataa aattacagat tactttaaac aaaatctttg
      121 aaaacaatat ttaattaatg agtaagaaaa caatttttaa tcttttacta tgtttctgtg
      181 ttgtttcaat tcatgcacaa aatctaaaac gttgtccgca gacaacatca aaagaagcag
      241 ctaagaagac cgaagaagca gagaagctat taaagtcagt taaaaattat gaggaaggtg
      301 ttgaattgtg taatgaggct attgaggctg acagcacatt tgcaaaaccc tatttgctta
      361 tgggtgatgc tgcatacagg aaaaaagatt tcaagttaat ggcaaaggct tataaaggcc
      421 ttatgaatgt ttgtcctgat gcatctgaaa aagcaatcta cagacttgca acatattatt
      481 ttgatacaaa gaagtatgaa gaagctattc ctgtttacac tacattcctt gaactttctc
      541 cgtcagatac caacgctgct gacgatgccg aagtgaaatt gttcagagca aaagcgtttt
      601 tacatccagt agattttaaa cctgtgttgg taaaaggatt aagcacacca gatcctgaat
      661 atctgccaat aatctctgct gataatgagt tgtgtttttt tacaagaaga tttgagtatc
      721 aaagtaaaaa catgctcact tcgaccagtg tagaaaagtt tatgttaagt cataatctaa
      781 atggcaaatg ggataccggt acaccaatgg atttaccttt taacaaacag aatactggta
      841 atgaaggtgg tccaactatt tctctcgaca acctgcactt gttttttact gttaatgtaa
      901 agggcaattt tgatatttat acttctgacc ttacaccaaa aggatggtct gaaccaagaa
      961 gcatagggaa agcaaatgac ccaaagtttt gggattcaca accatcactt tcaccagata
     1021 ataagacatt atattttgcc agtttcagag attctgttct tcagacaagt gatatttatg
     1081 tgactaaaaa aaataatggt gaatggaata gtgcaaatcc tatcagtgga ccaatcaata
     1141 cttcgcgaaa tgaaaaagca ccttttatgc atcccgatgg aaaaacactt tatttcagta
     1201 gtgatggtca ccccggcatg ggtggatatg atatttatta ttccaagaag gatgcaaacg
     1261 gtaactgggg aaaacctgtt aatctcggat atccgataaa taccgatgca gatgaagttg
     1321 gattttttgt aagtactgat ggtactaaag gtttttttgc aagtaataaa ttcggaacag
     1381 ggggttatga tatttactca ttcgacatgc cggtggaagc aaggcccgag aagatggttt
     1441 tattcaaagg ccaacttaaa aatgatgaca atgaagtgcc tgacagcgcc cgtatagaaa
     1501 ttcggaatat ttcatccaaa caggttgagc agatagatta cgatgcatac agtggaaaat
     1561 atgcaggtgt attgcaagct aattctgagt atttgttaac tattcaaaaa gatgattatg
     1621 catttacttc aaaacttatt gatgctgcaa aagttgattc gcctgcagtt actgttgagt
     1681 tagatctgag aaaaataaaa gaaggcgaag catatagttt aaatgatata ctttttgaaa
     1741 cagattctta tcagattaca gataaatcaa aagcagtaat tcaggatttt gcaggctttc
     1801 ttaaaagtaa tccaaagtta aaggttgcaa tttatggtca tactgataat tcaggaaaag
     1861 cagaagataa tttgttgttg tcaaaaaaca gggcgaaggc tgtttatgat tatttgattg
     1921 ctttaggcat tgaagcaaca cgactttcgc ataatggatt cggacaaaca aaaccggttg
     1981 ctgcaaatac gacagaaaaa ggtaagtcac tgaacagacg cacagagttt gtagtgttaa
     2041 aaaaatagtt ttatggagtt tgttattttg gttgatgaac aggatagcga agttggtgag
     2101 gcagaaaaaa tgcagactca taaacttgga ttattacaca gagctttttc agtattaatt
     2161 tacaattcca atggagaaat gcttttgcaa caaagagcag aaacaaaata ccatagtgga
     2221 ggactttggt caaatgcttg ttgtggtcat ccacgtcccg gtgaaaatat agaggatgcc
     2281 gcaaagagaa gacttaaaga ggagttgggc tttaattgtg atctgaagtt tagctttaaa
     2341 tttctttata aagtttcttt tgaaaatagt cttaaagagc atgaactgga tcatgttttc
     2401 acagggagtt atgaaagtga agtacttcct gataaaagtg aagttcaagc cttccggtgg
     2461 ataacaatga ataatctgaa gaatgaaatt acagaaaatc ctgataaatt ttctgtttgg
     2521 tttcttaaaa ttatttcgga gttgtaattc cttgttattt agctgataat attgaaataa
     2581 attaccgaat catacgtttt gcttgaatgc aaatccttat ttttggttcc tatgaataca
     2641 ttcaatttca gatgcaggaa ttttatcctg tcttttttat ttttcagtat gcctgttctg
     2701 gcatttgcgc agtctggtca ggaaaaactt gttcaattaa gtggtgtaat agttacaggg
     2761 gatagtttaa gtccggtacc cta
//
LOCUS       DAORQT010000212         2782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7238_length_2782_cov_6.339934, whole genome shotgun sequence.
ACCESSION   DAORQT010000212 DAORQT010000000
VERSION     DAORQT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7238_length_2782_cov_6.339934"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK134_07075"
     CDS             <1..197
                     /locus_tag="PK134_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38645.1"
                     /translation="VTLGVWEVENFPVNLHVTVMALAKVNDNYFFSKSSVIVSTDVSI
                     PLVMNSITLPALKTELEQLP"
     gene            complement(194..1789)
                     /locus_tag="PK134_07080"
     CDS             complement(194..1789)
                     /locus_tag="PK134_07080"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRB38646.1"
                     /translation="MSAVKYVFVTGGVSSSLGKGIISASLAKLLQARGYRVTIQKLDP
                     YINVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQTVIN
                     KEREGAFLGKTVQVIPHITDEIKRRIKILGETGEYDIVITEIGGTVGDIESLPFVESI
                     RQMIWEMGSNCCVIHLTLLPYLNVTGELKTKPTQHSVKTMLEYGVQPDILVCRSEKHI
                     TADLRRKIALFCNVNVNAVIEAVDASTIYEVPLHMLKEQLDKVVLNKLKLSFKQEPDL
                     TAWKAFLGKLKHPVSEVKIGLIGKYIELKDAYKSIAEAFIHAGVANECKLNIEWIHSE
                     KLKGLSGILVAPGFGDRGIEGKIAAIKYARENKIPFLGICLGMQCAVVEFARNVLGLK
                     DAHSSEMNKETKNPVIDLMAKQKEISSKGGTMRLGEYPCALAKGSKVAQAYGKTKIYE
                     RHRHRYEFNSKYQKRFEDAGMIATGINPADNLVEIVEIKNHPWFVGVQFHPEYKSTVE
                     NPHPLFVRFVKAASEFAKGAIQI"
     gene            2061..2552
                     /locus_tag="PK134_07085"
     CDS             2061..2552
                     /locus_tag="PK134_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38647.1"
                     /translation="MKKIYAFVIVLLIYSFTNSVSAQSQNAIDFDGLDDQVVSVNASS
                     LIANSSQISLSFWVYPRNPVPGFPDFDGLAGFRDNTVADFYVLQYSSTAVEARFRNSA
                     GINFDILSQGLQLNTWQQFALTYDGSYLRLFRDGLITDSVSASGVISSTATSFYMGNL
                     VFR"
     gene            2539..>2782
                     /locus_tag="PK134_07090"
     CDS             2539..>2782
                     /locus_tag="PK134_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38648.1"
                     /translation="MFLDEVALWNRALTPQEILCIYKNKVDATSAGLQLYYDMNTGTA
                     GGNNSGILTIPDISNHINGVLQNFSMNGPSSNFVNGI"
BASE COUNT          811 a          552 c          497 g          922 t
ORIGIN      
        1 ctgttacctt aggcgtgtgg gaagtagaaa attttcctgt caatctgcat gtaacggtta
       61 tggcattggc gaaggtcaac gacaattatt tcttttctaa atcatctgtc atcgtcagca
      121 ctgatgtatc cataccgtta gtaatgaatt ctattacctt accggcatta aaaactgaat
      181 tagagcaact gccttaaatc tgaatggcgc ctttggcaaa ttcgctggct gcctttacaa
      241 atcttacaaa tagaggatgt gggttttcta ccgtgctctt atattcggga tgaaactgaa
      301 ctccaacaaa ccaaggatga tttttaattt caacaatctc tactaagttg tcggcaggat
      361 ttattcctgt ggcaatcatg cctgcatctt caaatctttt ctgatacttg ctgttaaact
      421 cataacgatg gcgatggcgt tcatagattt tagtttttcc ataggcttga gccaccttac
      481 tgccctttgc aagtgcacaa ggatattcgc ccaaacgcat ggtgccacct tttgaagaaa
      541 tttctttttg ttttgccatc aaatcaataa caggattttt tgtctcttta ttcatttcag
      601 agctgtgtgc atctttcagt ccaagtacat tgcgggcaaa ctctactact gcacattgca
      661 tacctaagca aattcccaga aaaggaattt tattttctct agcatatttt atagctgcaa
      721 ttttgccttc aatacctctg tcgccaaagc cgggagccac caaaattcct gacaagcctt
      781 ttagcttttc ggaatgtatc cactcaatat tcaacttgca ctcattagca acgcctgcat
      841 gaataaatgc ttcggcaata gatttataag catctttcag ctcaatatat tttcctatca
      901 acccgatttt tacttcagac acaggatgtt ttaatttccc taaaaaagct ttccatgctg
      961 tgaggtcagg ttcttgcttg aaggaaagtt ttagtttatt gagcacaact ttgtctaact
     1021 gctctttgag catgtgcagt ggaacttcgt aaatggtgct agcgtcaaca gcttcaataa
     1081 ctgcattgac atttacgtta cagaacaaag caatttttct gcgcaagtcg gcagtaatat
     1141 gtttttcgct gcggcaaact aaaatatcgg gctgcacacc atactcaagc atggttttta
     1201 cagaatgctg tgtgggctta gtctttaatt cgcctgtaac atttaagtat ggcaaaagtg
     1261 taagatgaat aacgcaacaa ttgctaccca tttcccaaat catctgacgg atagattcca
     1321 caaacggcaa tgactcaata tcaccaacag ttccaccaat ttcagtaatt acaatatcat
     1381 actcaccggt ttcgcctaat atttttatac gtcttttaat ttcatcggta atatgtggaa
     1441 taacctgcac tgttttacct aaaaatgctc cttcacgctc tttattaatt acggtttgat
     1501 aaatacggcc ggtggtaacg ttatttgcct gtgatgtggg gacgtttaaa aaacgttcgt
     1561 aatgcccaag gtcgaggtct gtttctgcac catcattggt aacataacat tctccatgtt
     1621 cataggggtt taatgtgccc gggtcaacat tgatgtaggg atctaatttc tgaatggtaa
     1681 ctctataacc gcgtgcctgc aacaatttgg caagtgaagc agaaataatt cctttgccta
     1741 atgatgaaga aacaccaccg gttacaaaaa catactttac agcgctcata agtaggggtg
     1801 tggtgatacg ggatgcaaaa gtaatgtttt gattcggctt tgcagaaaag aacttcaata
     1861 aatcagcttt atttaattac tttttacttt tcattagttt atacaatcaa tgcgatttcg
     1921 acttgccaca aaaattctta cttaaaattt acatgccttt aatacatccg taggtaacag
     1981 cgaattttaa ctacaacata atcaagaaaa taagtactga atgtttttat atttgtaaaa
     2041 attaataaac aataaaggag atgaaaaaaa tatatgcttt tgtaattgta ttgctgatat
     2101 attcatttac aaattccgta tcagctcaga gtcaaaatgc gattgatttt gacggcttag
     2161 acgatcaggt tgtttctgta aatgcctcgt cactcattgc taattcatcg caaatttcac
     2221 ttagcttttg ggtgtatcca cgcaatcctg ttcctggatt tcctgacttt gacggactgg
     2281 cagggttcag agataatacg gtagccgatt tctatgtttt gcagtattct tccacagccg
     2341 ttgaagctcg ttttagaaac agcgcaggaa taaattttga tattctttcg caaggacttc
     2401 aactcaacac atggcagcaa tttgcattaa cttatgatgg ctcatattta agacttttta
     2461 gagatggttt aattaccgat tccgtttcag catccggtgt aatttccagc actgctacat
     2521 cattttatat gggtaatctt gtttttagat gaagtggctc tttggaacag agcgctaaca
     2581 cctcaggaaa ttttatgtat ttataaaaat aaggtagatg ctacttctgc cggtttacaa
     2641 ctttattatg atatgaacac cggcacagca ggtggaaaca attccggtat tcttaccatt
     2701 cctgatattt caaaccatat caatggtgtt ttacaaaatt ttagcatgaa tggtccatca
     2761 tcaaattttg taaatggcat tg
//
LOCUS       DAORQT010000213         2766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7303_length_2766_cov_5.657691, whole genome shotgun sequence.
ACCESSION   DAORQT010000213 DAORQT010000000
VERSION     DAORQT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7303_length_2766_cov_5.657691"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..2146)
                     /locus_tag="PK134_07095"
     CDS             complement(53..2146)
                     /locus_tag="PK134_07095"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRB38649.1"
                     /translation="MKVFKLTLALLLLAVTSLYAISFTKYSDKLFSQEEYNTLIKKSA
                     LAVAQEMHFPERAFAEVYSIHKIEITERSIPFIRKKFLWLEKGYSTWPASESVASKIR
                     KEVYERELQRGVRDALYADQNYLNSIFTINESDRIISFHSDIKVSVNGKLTVVETIKI
                     YNGDGGSNDEIKRGIVRTFPTRYTTSLGFVSTVPFDFISATCDGKTENYKKESAENGV
                     RYYFGNADHFLDRGYYTYVLTYETAKQIIFHPDKDELYWNVNGTGWSFTSDKVSCKIT
                     FPEQTKIIENNCYTGVQGSAEKLCNYKITAPNVVEFSTQQLLNSNEGLTISTTIAKGV
                     LIAPDSTEKAFAAIKDNFILPALVAAILFLIGYNYKAWRKVGRDPEAGTIIPQFEPPQ
                     NFSPADCGFLITKSYKSHHFAASLVDCAVNRLVDIEVKEEGMIFKTPVYYFNRPASAT
                     NEDQILNLRNQWYGYDIERLYGQKAEKGTYNSTIASVYNGLESHLKDRMEFTKSKSGS
                     FKNLFTLNFNYIGWGILVLALSVVGMIIYFILFGGTPVLVLSSIGLILVGIIIQILFA
                     RWMQAYSVEGRKILDYVLGFKMYLETTEQKKFDTMNPPDLTIQLFEKYLPYAIALECE
                     NQWSERFEAIINKAIEGGYQPAYFNMHGSSLNMRTFTSGLSSGISNTIASASTPPSRS
                     SGGSGGGGSSGGGGG"
     gene            complement(2158..2709)
                     /locus_tag="PK134_07100"
     CDS             complement(2158..2709)
                     /locus_tag="PK134_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRB38650.1"
                     /translation="MMILGIVGGILLLLIVFLISIFNELTKERVLVQEAWSGIGAFLQ
                     QRLDAIPNLVEIVKGYAGHENKTLTDVIRARNESIAALTPEAQIEAAKNMSAAMLNFK
                     ALAENYPDLKANQNFIKLQEQLTELEEKINQSRRYYNGTVREYNQSIAVFPKNIVAGM
                     FGFNASAFFAEDTDAAKAPKISF"
BASE COUNT          837 a          566 c          467 g          896 t
ORIGIN      
        1 catccttaat acgtgaaaaa acagcatgag gttaccagcc tccgcctccg cctcaaccac
       61 ctccgcctcc ggaagaacct ccacctccgc tgccaccact gctgcggctt ggtggagttg
      121 atgctgatgc aatggtatta ctgataccac ttgaaagtcc agaagtaaat gtgcgcatat
      181 ttaaactgct gccgtgcatg ttgaaatatg ccggttgata gcctccttca atagctttgt
      241 taatgatagc ttcaaacctt tctgaccatt gattttcaca ttccaaagca atggcataag
      301 gcaaatactt ctcaaaaagc tgaatggtta aatcaggtgg attcattgta tcaaactttt
      361 tctgttctgt tgtttccaga tacattttaa agccaagtac ataatccaat attttccgac
      421 cctcaacaga ataggcctgc atccaccttg caaatagaat ttgaataatt attcccacta
      481 gaattaaacc aatagatgat aacactaaaa ccggtgttcc accaaaaaga ataaaataaa
      541 ttatcattcc aacaacactt aaagccaaaa caagtattcc ccagccaatg taattaaaat
      601 tcagtgtaaa caaattttta aaactgcctg acttactctt ggtaaactcc attctatctt
      661 ttaaatggct ttctaaacca ttataaaccg atgcaatggt tgaattgtaa gttccttttt
      721 ctgctttttg tccatatagt ctttcaatat catagccata ccattgattt cttaaattca
      781 gtatttgatc ttcattagtt gctgaagcgg gtctattgaa atagtaaacc ggagttttaa
      841 aaatcattcc ttcttctttg acttcaatat caaccaatct gttcacggca caatcaacta
      901 atgatgcggc aaagtgatgc gacttgtaac tctttgttat taagaaaccg caatcggcag
      961 gagaaaaatt ttgtggtggc tcaaactgag gaataatagt tcctgcctca gggtcacgac
     1021 caactttgcg ccatgctttg tagttatatc cgattaaaaa aagtattgcc gccaccaatg
     1081 ctggaagtat aaagttgtct ttaattgctg caaatgcttt ctctgttgag tcaggagcaa
     1141 ttaaaactcc ttttgcgatg gttgttgaaa tcgtcagacc ttcattactg tttagtaatt
     1201 gctgcgtact gaactcaaca acatttggtg ctgttatttt ataattacat agtttctccg
     1261 cagagccttg cacaccggtg taacaattgt tttcaataat ttttgtttgc tcaggaaaag
     1321 ttattttaca actcacttta tctgatgtaa aactccatcc tgttccgtta acattccaat
     1381 acaactcatc tttatcagga tgaaaaatta tctgcttggc tgtttcataa gtcaaaacat
     1441 aagtgtaata acctctgtca agaaaatgat cggcattgcc aaagtagtaa cgtacaccgt
     1501 tttctgcact ttctttttta tagttttcag ttttaccatc gcatgtggct gaaataaaat
     1561 caaatggtac tgtagagaca aatcccaatg atgttgtata acgtgtaggg aaagtacgta
     1621 ctatgccacg ctttatttca tcgttgctgc caccatcgcc attgtaaatt tttattgttt
     1681 caacaactgt cagcttgccg tttactgaaa ctttaatatc agaatgaaaa gaaataattc
     1741 tgtctgactc attgatggtg aaaattgagt tcagataatt ctgatcggca tacagcgcat
     1801 cacgaacacc acgttgtaac tccctttcat agacttcttt tcgtatttta cttgcaacag
     1861 attccgatgc aggccaggtg ctataaccct tttcgagcca taaaaacttt tttctgataa
     1921 atggaatgga acgctctgta atctctattt tatggataga atatacttca gcaaaagcac
     1981 gttcaggaaa gtgcatttct tgtgcaacag ccaaagccga cttcttaatg agcgtattat
     2041 attcttcctg agaaaaaagt ttatcggaat atttagtaaa acttattgca tatagtgatg
     2101 tcactgcaag aagcaatagt gctaatgtca gtttaaagac cttcacagca gttactttta
     2161 aaatgaaatt tttggtgctt ttgcagcatc tgtatcttca gcaaaaaacg cagaagcatt
     2221 aaagccaaac attcctgcaa caatattctt aggaaacact gcaatgctct gattatattc
     2281 ccggacagtg ccattataat atctgcggct ctgattgatt ttttcttcca actccgtaag
     2341 ttgctcttgt agttttataa aattctgatt ggctttcagg tcagggtaat tttctgccag
     2401 tgccttgaaa ttgagcatgg cagcactcat attttttgct gcttcaatct gcgcttccgg
     2461 tgtcagtgca gcgatagatt cattacgggc acgaattacg tctgttagcg ttttattttc
     2521 atgacctgca tatcccttta ctatttctac aagatttgga atagcatcta atcgttgttg
     2581 cagaaatgca ccaatgccac tccacgcttc ctgcacaagc acgcgctctt tggtgagttc
     2641 attaaaaata gaaataagga aaactataag tagcaacagt atgccaccga ctatgccaag
     2701 tatcatcata taagttgttt tttgtaaaag tagaaaaaat attcaaatat tgatatggtg
     2761 ctgaat
//
LOCUS       DAORQT010000214         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7318_length_2764_cov_7.423403, whole genome shotgun sequence.
ACCESSION   DAORQT010000214 DAORQT010000000
VERSION     DAORQT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7318_length_2764_cov_7.423403"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PK134_07105"
     CDS             <1..652
                     /locus_tag="PK134_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38651.1"
                     /translation="VNTLIKSAVMNAETVSSTLTKASDTVYTLGVDSEYMQKRKKLSD
                     TPDSFTNKELVNYFTSVNRISTGFKIKKYAEQSKSRLIGIKVHELLADFRSESDLKAI
                     EKHLSNLQLPDDEKNELLNGVQTTCNFLSEKKWVSPHFKIYNEHEMADAEGNILRPDR
                     LMIEGDNAVVIDYKTGAIETSHVQQIANYKEVLLDAGFKDVKTFLYYLEGKELVEA"
     gene            complement(660..1058)
                     /locus_tag="PK134_07110"
     CDS             complement(660..1058)
                     /locus_tag="PK134_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38652.1"
                     /translation="MKNVHSKSQIIIFDNYGNILKSCDTIFQVSDLIGKNIMKQFPLL
                     NCYEPEIRNLSKSKEPFFVPCIAFSFANFKSICDFVFIRHEELNEITISWMISDNYVH
                     FKERLNKPINLPQTKSKPMVTEFVNRWHGK"
     gene            complement(1060..1575)
                     /locus_tag="PK134_07115"
     CDS             complement(1060..1575)
                     /locus_tag="PK134_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRB38653.1"
                     /translation="MQNKKILITGYSQCGKSSIVNRLVNNRFHHSYSEDSQLCIARKE
                     LAINKKKINFVIWTAPGSVSNLKSFKSYYLGTDALIHVVDVNNPSTFSDLDAWISHYQ
                     KFLPGTPVYLTCNKIESNINLDCSDFFTQFDCYRTFFTSAKNNFMINEMFQTIAADLL
                     NEPFFQKIKIY"
     gene            complement(1647..>2764)
                     /locus_tag="PK134_07120"
     CDS             complement(1647..>2764)
                     /locus_tag="PK134_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38654.1"
                     /translation="YTVTLKVTNEGLCSDSITKLNFIAAQNNQPITPSEINYLTVLDS
                     NAIRISWQPSSATNLNRFELYRSTDDKNYNLIYTDTNTHNSTLNLQLAFIDNNVTTNQ
                     NRYYYKLQTINNCNNSYPLDSSISHASILLTTEPDTMSNMLSWLPYIGKTISQYSIYR
                     STDGLNYDLIDEIAGTEDFYIDSTAYCPQEYSYKVEAQLDSSIYTSESNNDASVPTSF
                     VNQITTSIIRSTVVDNNSILTEWHEGNPFPELVYYYLIYKSSDKDPGNFVLIDSVNNS
                     TKYYLDRDVDVNATNYQYKIEVENICQQRTGLTDLSGNILLQGKLDDMNNSRLEWTPY
                     KGWKEGVERYEIQKQDENGNWKTIKSVDGKTHHMEEE"
BASE COUNT          846 a          406 c          514 g          998 t
ORIGIN      
        1 agttaataca ttaattaagt cggcagtaat gaatgctgaa actgttagtt caactttgac
       61 aaaggcaagt gatactgttt acactttggg agttgattca gaatatatgc aaaagagaaa
      121 gaaactatcc gacacacctg atagttttac taataaggag ctggtaaatt attttacaag
      181 tgtaaacagg ataagcaccg gattcaaaat taaaaagtat gctgaacaaa gtaaaagccg
      241 cttaataggg attaaagttc atgagttgct ggctgatttt cgcagtgaat ctgatttaaa
      301 agcaatagag aagcatttaa gtaatcttca attaccggat gacgaaaaaa atgagttgct
      361 gaatggcgtg caaactactt gcaattttct ctctgagaaa aaatgggtta gtccgcattt
      421 taaaatttat aatgaacatg aaatggccga tgcagaaggt aatattttaa gacctgacag
      481 attgatgatt gaaggggata atgctgtggt tattgattat aagacaggag ccattgaaac
      541 ttctcatgtg caacaaatag ctaattacaa ggaagttttg ctggatgcgg gcttcaagga
      601 tgtgaagact tttttatatt atctggaagg gaaagaatta gttgaggctt aaatgctttc
      661 tattttccat gccaacggtt gacaaattct gtaaccattg gtttagattt agtttgagga
      721 agattaatcg gtttgttcaa ccgttcttta aagtgaacat aattgtcaga aatcatccag
      781 gatatggtga tctcattcaa ttcttcatgt cttataaaaa caaagtcgca aatactttta
      841 aagttagcaa aagagaatgc tatgcaagga acaaaaaatg gctctttact ttttgaaaga
      901 tttctaattt caggttcata gcaattcaac agcggaaatt gcttcatgat gttttttcct
      961 atcaaatcgc ttacctgaaa aattgtatca cagcttttaa gaatgttgcc atagttatca
     1021 aaaataataa tctggctttt gctgtgaacg tttttcatat cagtaaattt tgattttttg
     1081 aaagaatggc tcattcagta aatcagccgc tattgtttga aacatttcat taatcatgaa
     1141 attgtttttt gctgatgtaa aaaaagttct gtagcaatca aactgtgtaa agaaatcact
     1201 gcagtcaaga ttgatattcg attcaatttt attgcaagtt aagtaaacag gcgtgcctgg
     1261 aagaaatttc tgataatgac taatccatgc atcaagatct gaaaatgttg atgggttgtt
     1321 tacatcaact acatgtatta atgcatctgt tccaagataa tatgacttga acgattttag
     1381 attcgaaaca cttcctggag ctgtccagat aacaaaattg atctttttct tatttatagc
     1441 caactctttt cttgcaatgc ataattgact atcttctgaa taggaatgat gaaatctgtt
     1501 attcaccaat cggttcacaa tagaactttt accgcattgg ctatatcctg ttatgagtat
     1561 ttttttattc tgcatgccga ttgtttatag tgtttatatg cagaaaagag aaaaaggttg
     1621 cagtattttg tttgaacttc tgaaaattat tcttcttcca tgtgatgggt tttgccatct
     1681 actgatttta tggttttcca attaccgttt tcatcttgtt tttgtatttc atagcgttca
     1741 acaccttctt tccagccttt gtatggagtc cattctaatc tgctgttgtt catgtcatct
     1801 aatttgcctt gtagaagaat gtttcctgat aaatctgtca gacctgttct ttgctgacat
     1861 atattttcta cttcaatttt gtattgataa tttgtagcat tgacatccac atctctgtca
     1921 agatagtatt ttgtgctgtt gtttaccgaa tctatcagaa caaaatttcc cgggtctttg
     1981 tctgatgatt tataaattaa ataatagtaa accaactcag gaaatgggtt gccttcatgc
     2041 cattctgtta aaatactgtt gttgtcaacc acagttgaac gaatgattga tgtggttatc
     2101 tgatttacaa aacttgttgg taccgatgca tcattattgc tttcagaagt gtatatgctg
     2161 ctgtctaatt gtgcttcaac tttataactg tattcctgag gacaatatgc tgtagaatca
     2221 atataaaaat cttctgttcc tgcaatttca tctatcaggt cataattcaa tccatcagta
     2281 cttcggtaaa tcgagtattg tgaaattgtc ttaccaatat aaggtaacca tgagagcatg
     2341 tttgacattg tgtcgggctc tgttgttaat agaattgatg catgggatat tgaactatcc
     2401 aatggatatg aattgttgca attattgatt gtttgaagtt tgtaataata gcgattctga
     2461 tttgttgtta cattattgtc aataaatgct aattgcagat tcaatgtgct gttgtgtgta
     2521 tttgtatctg tgtaaatcag attgtaattt ttatcgtcag ttgatctgta taattcaaat
     2581 ctgtttagat tggtagctga tgatggttgc catgatattc tgattgcatt gctatcgagt
     2641 acggtgaggt aatttatttc tgaaggtgtt attggttgat tgttttgtgc agctatgaag
     2701 ttgagtttag taatactatc actgcaaagg ccttcattag tgacttttaa tgtcactgta
     2761 taat
//
LOCUS       DAORQT010000215         2755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7354_length_2755_cov_7.238148, whole genome shotgun sequence.
ACCESSION   DAORQT010000215 DAORQT010000000
VERSION     DAORQT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7354_length_2755_cov_7.238148"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..2525)
                     /gene="ccoN"
                     /locus_tag="PK134_07125"
     CDS             complement(399..2525)
                     /gene="ccoN"
                     /locus_tag="PK134_07125"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRB38655.1"
                     /translation="MTIEKFRYDNKIVAMFAWATVIWAIVGMLVGLLAAFQLPFPALN
                     FDAHTTFGRIRPLHTNAIIFAFVGNGMFMGIYYSLQRLLKARMYSDVLSYIHFWGWQL
                     IIVGAAITLPLGITASKEYAELEWPLDIAIAIVWVIFGINMFGTLVRRRERHLYVAIW
                     FYIATVVTVAVLHIVNSLAIPVTLFKSYSLYAGVQDALVQWWYGHNAVAFFLTTPYLG
                     LMYYFLPKAANRPVYSYRLSIIHFWSLIFLYIWAGPHHLLYTALPDWAQSLGVVFSIM
                     LLAPSWGGMINGLLTLRGAWDRVRDNPELKFYVVAVTAYGMATFEGPMLSLKSVNAIS
                     HFTDWTIAHVHIGALGWNGFLTFGILYWLIPKLFNTSLYSKKLAGIHFWLGTLGIVFY
                     AIPLYWAGITQHFMWKEFTEDGVLRYPNFLETVVQVVPMYKLRALGGSLYLLGSLVMV
                     YNLIKTARAGKMVANEDAEAPALEKNYVAHDDHWHRWIERRPAQMLIGSLIVILIGGM
                     VEIIPTFMVKSNVPTIASVKPYTPLELQGRDIYVREGCYTCHSQMVRPFRYETERYGE
                     YSKPGEFVYDHPFQWGSKRTGPDLHREGGKYPNSWHYNHMLDPQTMSPGSIMPSYAFM
                     IDDDLDTTTTAAKIRAMQKLGVPYPEGYDAQANKDLMKQANSIASNLEKDGIKVMGNK
                     EIVALIAYLQRLGVDIKAEKTTASVN"
     gene            complement(2530..2715)
                     /gene="ccoS"
                     /locus_tag="PK134_07130"
     CDS             complement(2530..2715)
                     /gene="ccoS"
                     /locus_tag="PK134_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRB38656.1"
                     /translation="MSVLIILIGASLVVAAGFLVSFFWAVRSGQYDDDYSPSVRILFD
                     NDKTLTSDKSNSNITDN"
BASE COUNT          884 a          563 c          496 g          812 t
ORIGIN      
        1 gtttaagact gcttctatat aatgccaata ttgtggcaag taaaataatt actacaaatg
       61 caatgagata agtatttgca ttcattaacc aatccggagg ctgagcggct gcaacttttg
      121 ttgtttctgt tgcctgtgta tcaccggccc atgcagataa tgagatgaac attatcaaac
      181 atattaaatt aattatcttt ttcattactt cagattttaa tcgtttgttt cttctagtgg
      241 cagcttactt atagactcaa agtcttcttt ctttgatttc aaaacccaaa caccaacaag
      301 ggtaaaaaaa acaaagaaaa taacgaagga aaatacagga tagatactga tatcctttat
      361 tgttgataat acttctttat acatattctt ctttattttt aattaactga ggctgtagtt
      421 ttttctgctt tgatatcaac acctaatcgt tgcaggtatg ctattaaagc tacaatttct
      481 ttattaccca ttactttgat tccatctttt tcaagattag atgcaatact gtttgcctgc
      541 ttcatcagat ctttgtttgc ctgtgcatca taaccttcag gatagggcac acctaacttt
      601 tgcatagcac gaatttttgc tgcagtagtg gtggtatcta aatcatcatc aatcataaat
      661 gcataggatg gcataatact tccgggtgac atggtttgcg gatcgagcat gtgattgtag
      721 tgccatgagt ttggatattt ccctccttca cgatgcaggt cagggccggt tcgcttacta
      781 ccccattgaa acggatgatc atagacaaat tcaccaggct tggaatattc accatagcgt
      841 tcagtttcat agcggaacgg tcgcaccatc tgtgagtggc atgtataaca tccttctcgc
      901 acataaatat cacgtccctg taattcaagt ggtgtgtatg gtttaacgct tgctattgtt
      961 ggtacgtttg acttcaccat aaaagttgga ataatttcaa ccattcctcc tatgagaatt
     1021 acaataagac ttccgataag catctgagca ggtctgcgtt ctatccaacg atgccagtgg
     1081 tcatcatgag caacataatt tttttctaat gccggtgctt ctgcatcttc attagcaacc
     1141 atttttccag cacgtgcagt tttaataagg ttataaacca tcaccaaaga accaagcaga
     1201 taaagtgatc cgcctaaagc acgtagttta tacattggca ccacctgcac tactgtttca
     1261 aggaaatttg gataacgcaa cacaccatct tcagtaaatt ctttccacat gaaatgttgt
     1321 gtaatacctg cccagtaaag tggaattgca tagaagacaa tgccaagtgt cccaagccag
     1381 aaatgtattc ccgctaattt tttagaatat aaacttgtgt tgaataattt tggaatcagc
     1441 caataaagaa ttccgaaagt cagaaatccg ttccatccaa gtgcgccaat gtgtacgtgt
     1501 gcaattgtcc aatcggtaaa gtgactgatg gcattgacag attttaaaga aagcataggc
     1561 ccttcgaatg ttgccatacc ataagcagtt actgcaacaa cataaaattt aagttccgga
     1621 ttatcgcgta ctctgtccca tgcaccgcgc agtgtcagca atccgtttat cataccaccc
     1681 catgatggag caagtaacat tattgaaaac acaacaccta aggattgtgc ccaatcgggc
     1741 aatgcagtat aaagcaagtg gtgtggtcct gcccatatat aaagaaatat taatgaccaa
     1801 aagtgaatga ttgaaagacg ataggaatat acgggacggt tagcagcttt aggaagaaaa
     1861 taatacatca atcccagata aggtgttgta agaaaaaatg caactgcatt gtgtccatac
     1921 caccattgca ctaatgcatc ctgtactcca gcataaagtg agtagctttt aaataatgtt
     1981 acaggaatag caagagagtt tacaatgtgc aacacagcaa cagtaacaac tgttgctata
     2041 taaaaccata tagctacata caaatgccgt tcacgtctgc gaactaaagt gccaaacatg
     2101 tttatgccaa atattaccca aactatggca attgcaatat caagtggcca ttctaattct
     2161 gcatattctt tgctggctgt aattcctaat ggaagggtaa ttgctgcacc tacaatgatt
     2221 aattgccaac cccaaaagtg aatatagctt aacacatcac tgtacattct tgctttgagt
     2281 aaacgttgca acgaataata aattcccata aacattccat tacccacaaa agcaaaaatg
     2341 attgcattgg tgtgtagcgg tctgatacga ccaaaagttg tgtgtgcatc aaaatttaat
     2401 gcaggaaaag gaagttggaa agcggctaac aggcctacaa gcatgcccac aatagcccat
     2461 attactgtag cccaagcaaa catggcaaca atcttgttgt cgtagcgaaa cttttctatt
     2521 gtcataattt tagttatctg ttatgtttga atttgattta tctgatgtta aagttttgtc
     2581 attgtcaaaa agtatgcgca cagaaggcga gtaatcatca tcatactgcc cgctgcgaac
     2641 ggcccaaaaa aaagaaacca agaagcctgc tgccaccacc aggctagctc caataagtat
     2701 aataagtaca ctcataaaaa acggtcacaa acctactccg cctaaagtag ttatt
//
LOCUS       DAORQT010000216         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7375_length_2751_cov_13.156528, whole genome shotgun sequence.
ACCESSION   DAORQT010000216 DAORQT010000000
VERSION     DAORQT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_2751_cov_13.156528"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PK134_07135"
     CDS             <1..920
                     /locus_tag="PK134_07135"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38657.1"
                     /translation="YIQGQAQIYSNGTIINHPGVGQGGADISCFHHGLASLGFGQQLL
                     EGYRIADDFNIPAGQIWTIDSIRFYAYQSNSGIVSTINAVNCEIFNGSPASGGTIVLG
                     DTITNLLQNTRFSNIYRTVDGQFNSVSRPVMYSVIVPQSAWTLTSGNYWLSWQSGGNP
                     SLSGPWVPPLTDTLNTTTGDGLLYIPDSSMWLPALDVALSTPQGFPFEIYGTITTSIN
                     TIEVEDALTILPNPSKGNFMINASFANRCNLEINIMDLQGRSIYSENVSDVSVLTKNV
                     VLNDVSKGIYLLKITTDTGKTSIKKITIN"
     gene            complement(995..1516)
                     /locus_tag="PK134_07140"
     CDS             complement(995..1516)
                     /locus_tag="PK134_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38658.1"
                     /translation="MSIILLFTACQKDYNDPGTGPSQYYGTRSGVSIRAILLAQMPAT
                     DPSGNSWDVADSVDTAGRADIYYRFKDIDSIDYPEFVQNFHFDNVNPDSLPLPYFLTT
                     PYPLRAIGSNVQFDIYDFEKSLSSPAIDSTLMKSFNFSVVAGDSLIENPYPDSLVLAE
                     DGYVVLLYLNWKK"
     gene            complement(1630..1791)
                     /gene="rpmH"
                     /locus_tag="PK134_07145"
     CDS             complement(1630..1791)
                     /gene="rpmH"
                     /locus_tag="PK134_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRB38659.1"
                     /translation="MVRTYQPSKRKRKNKHGFRERMATANGRRVLASRRAKGRKKLTV
                     SSESKVRGK"
     gene            complement(1858..2493)
                     /gene="rsmG"
                     /locus_tag="PK134_07150"
     CDS             complement(1858..2493)
                     /gene="rsmG"
                     /locus_tag="PK134_07150"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRB38660.1"
                     /translation="MSSGVSLLQRYFPELPLEKQEKFVLFAELFKVWNEKINLVSRKD
                     IDQFYLHHLLHSLVIAKFIDFGKGTKVMDIGTGGGLPGLPLAIYFPETNFYLVDSIRK
                     KTEAVKEMANQLSLSNVTVFNKRAEDVSEKVDFVVSRATAPLSDLVKWSRGRINSKGK
                     ASIPNGLICLKGGDLTEELKPFENKLFTENVTAFIQEEFYKEKKIIYLPLV"
     gene            complement(2497..>2751)
                     /locus_tag="PK134_07155"
     CDS             complement(2497..>2751)
                     /locus_tag="PK134_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB38661.1"
                     /translation="LEIKSDTIGPEEKLMKKQKMETMREVVDKLKPRYRTLIELRYFQ
                     ELSYEEIATNMDLPLGTVKAQLFRAREFLYQIMKNSEHRP"
BASE COUNT          877 a          496 c          470 g          908 t
ORIGIN      
        1 gctatattca gggtcaggca cagatttatt ccaatggcac tattattaat cacccgggtg
       61 taggtcaagg tggtgcagat atatcctgtt ttcatcatgg attagcttca ttaggattcg
      121 ggcaacaatt gcttgaaggc tatagaatag cagatgattt taatatcccg gcaggtcaaa
      181 tttggacaat tgactctatt aggttttatg catatcagag taattcagga attgtatcta
      241 caataaatgc tgtaaattgt gaaatcttta atggctctcc ggcatcaggc ggtacaattg
      301 ttttaggtga tacaataact aatctgttac aaaacactcg cttttcaaat atttacagga
      361 ctgtagatgg tcagtttaat tccgtgtcac gtccggtaat gtacagtgta attgtaccac
      421 aaagtgcatg gacacttact tcaggtaatt attggttaag ttggcagagt ggtggaaatc
      481 catctctctc aggaccatgg gtaccgcctt taactgatac tttaaatact actacaggag
      541 acggattatt atatattcct gatagctcta tgtggcttcc tgcattagat gttgctctat
      601 caacacctca aggatttcct tttgaaatat atggaactat tacaacttca attaatacaa
      661 tagaagtaga agacgcactt actattctac caaatccatc aaaaggaaat tttatgatta
      721 atgcttcttt tgcaaaccgc tgtaatctgg aaattaatat aatggatttg caaggcagaa
      781 gcatctactc tgaaaatgtt agtgatgttt ctgttttaac taaaaatgtt gttttgaatg
      841 acgtttccaa agggatttat ctgttgaaaa ttactacaga tacaggaaaa acaagtataa
      901 agaagattac aataaattaa ttgattaaag tacttttaga aaaagctgca cccttataga
      961 gtgcagcttt tcatttttat aaataatcac aacattactt tttccagttc aaataaagca
     1021 gaactacata accatcttct gccagtacca aactatcagg ataaggattc tcaattagtg
     1081 aatcgcctgc aacaactgaa aagttaaatg atttcattaa ggttgagtca atagctggcg
     1141 atgaaaggct cttctcaaaa tcataaatat caaactgaac attgctgcca atggcacgta
     1201 atggataagg cgtagttaaa aaatatggca gcggcaaact atcaggattt acattgtcaa
     1261 aatgaaaatt ctgaacaaat tcaggatagt caatagagtc tatgtcttta aaacggtaat
     1321 aaatatctgc acgacctgct gtgtcaacag agtctgctac atcccaacta tttcctgatg
     1381 gatctgtagc aggcatttgc gccaataaaa tagccctgat agaaacaccg ctgcgtgttc
     1441 cataatattg cgatgggccg gtgccggggt cattataatc tttctgacaa gcagtaaaca
     1501 agagaataat agacaaataa aagagacctg atttgaaatt catgattaca agaaacttta
     1561 ataattggtg ctaaaataaa aaagccacct taaagaaggc agctttttga tattttataa
     1621 tgtagttatt tatttaccac gaactttact ttcacttgaa acagtcagtt ttttacgacc
     1681 ttttgctctg cgtgaagcta acactctgcg accattagca gttgccattc tttcacgaaa
     1741 accatgtttg ttttttctct tacgctttga gggttgatat gtccttacca taatacttca
     1801 gtttttatta atgctgcaaa attcttttta gaggcggcaa agatataatt aattctttta
     1861 tactaaaggc aagtagatta tttttttttc tttataaaat tcttcctgta taaatgccgt
     1921 aacattttct gtaaatagct tattttcaaa tggttttagc tcttcggtta aatctccccc
     1981 ctttaaacaa ataagtccat ttgggattga tgccttgcct ttagaattaa ttctacccct
     2041 actccatttt accaaatctg agagtggtgc cgtagctctg gaaacaacaa agtcaacttt
     2101 ttctgataca tcttcggctc gtttgttaaa tacagttaca ttagataatg aaagttgatt
     2161 ggccatttct ttaacggctt cagtcttttt acgaatagaa tcaaccagat aaaagttagt
     2221 ttcaggaaaa taaatagcta atggtaaacc cggtaatccg cctccggtgc caatgtccat
     2281 gactttagtt cctttaccaa aatcaataaa ctttgctatg acaagagaat gtaataagtg
     2341 gtgtaaataa aactgatcta tatcttttct ggaaacaagg tttatcttct cattccatac
     2401 tttaaataac tcagcaaata agacaaattt ttcctgcttt tctaaaggta attccggaaa
     2461 gtagcgttgc aataatgaaa ctccgcttga catcttttat ggacgatgtt cagagttttt
     2521 cattatctga tacagaaatt cacgcgccct gaaaagttgg gctttaacag ttccaagagg
     2581 taaatccatg ttcgttgcaa tttcttcata actcaactcc tgaaaataac ggagttcaat
     2641 aagtgtccgg tatcttggtt ttaacttatc aacaacctca cgcatggttt ccattttttg
     2701 ctttttcatc agcttttctt caggacctat ggtgtcagat tttatttcca a
//
LOCUS       DAORQT010000217         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7430_length_2739_cov_7.464232, whole genome shotgun sequence.
ACCESSION   DAORQT010000217 DAORQT010000000
VERSION     DAORQT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7430_length_2739_cov_7.464232"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK134_07160"
     CDS             complement(<1..256)
                     /locus_tag="PK134_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRB38662.1"
                     /translation="MRLLIAFILFIWIPVFAKAQQEPLNNPAELTEKLKQASISNNTI
                     KADFIEQRFMSALKEPQTSYGIFYYKKENKLRWEKTKPTNY"
     gene            complement(253..1005)
                     /locus_tag="PK134_07165"
     CDS             complement(253..1005)
                     /locus_tag="PK134_07165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB38663.1"
                     /translation="MKHKIVTVIFILCIIALFVFLYQDKSFFYLLAALIFLYLTITAL
                     GSYQIKWNYFIKSVNTGKPEGISLTFDDGPDPKVTKKILDVLELAGAKATFFVIGKKA
                     EQYPDIINLIVSKGHTIGNHSWSHHAGIGMFSKQKLLADFNQCSQKIEELTGQKPVYL
                     RPPFGVTNPRYEYVLKNTGLKSIGWSIRSYDTSAKNEQKLLHRITSQLKPGAIVLLHD
                     TKDITLAILPALLNYCNQNNIAIKPLAELIEI"
     gene            complement(995..1960)
                     /locus_tag="PK134_07170"
     CDS             complement(995..1960)
                     /locus_tag="PK134_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HRB38664.1"
                     /translation="MYCIQSASTISHQPSFASKGFSSNLSKEIESLIKPDYKEYVPPA
                     LIRRMSEILRMSVACSNLALKEAGIEQPDAIITGTGLGCLYDTEKFLNNVITIEGGLL
                     PPTSFIQSTHNTIAGQISLVLGNHQYNMTHTQNSLSFEHALLDTMLMLDEKNGNILIG
                     SADEDIEALHPIAAHFKLNSLPLTSGASFFVLSKNKNEKTLAVIEACHTSAFYSSLEN
                     EIKDFMSTSTDKQIDFILNDGRNKDITRLIETLFPDKCVINYSEICGLYATNSAFAMH
                     LATDIICNKTKQVYGKTIANHKRALIINTLLPARAGFIMIKSNEA"
     gene            complement(1963..>2739)
                     /locus_tag="PK134_07175"
     CDS             complement(1963..>2739)
                     /locus_tag="PK134_07175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRB38665.1"
                     /translation="ENIAAYLGFSGYINTLSTACSSAANAIMLAARMIEQGKMDRVLV
                     GGTDPITDFTIKGFQSLMIYDDEWCKPFDHNRNGLNLGEGAGFLLLESDHSINISGNE
                     PICSLTGWHNAADAYHQTASSPDGKGATIAMKNALKKAGLGPKEISYINAHGTGTKNN
                     DLSESIAIKNVFGESIPPFSSTKAYTGHTLAAAGAIEAVYSVLAIKNQIHFAGLNYND
                     PLEETLMHPVTEPTKAVTKHVMSNSFGFGGNNSSLIFSGI"
BASE COUNT          815 a          479 c          496 g          949 t
ORIGIN      
        1 cataatttgt gggttttgtc ttttcccatc gcaatttgtt ttctttcttg taataaaaaa
       61 taccataaga tgtttgtggc tctttcaatg cagacataaa tcgttgttca ataaaatctg
      121 ctttaattgt attgtttgaa atgcttgctt gcttgagttt ttctgttaac tcagccggat
      181 tgttcaatgg ttcctgctgt gctttggcaa aaacaggtat ccaaatgaat agtataaatg
      241 caattaacaa tctcatattt caattaattc agctaacggt ttaatggcaa tgttattttg
      301 attacaatag tttagcaatg ccggtaaaat ggcaagtgtt atatcttttg tgtcatgcaa
      361 aagtacaatt gctccgggtt tcaattgtga agttatccgg tgtaaaagtt tctgctcatt
      421 ttttgctgag gtgtcataac ttctgatact ccatccaata gatttcaatc ctgtattttt
      481 caacacatac tcatatctgg gattagttac tccaaatggt ggtcttagat aaactggttt
      541 ttgtcctgta agctcttcta ttttttgact gcattgatta aaatcagcca acaacttttg
      601 ctttgaaaac atgccaatcc ctgcatgatg tgaccatgaa tggttgccta tagtgtggcc
      661 cttgctgaca attagattaa taatgtccgg atattgttca gcctttttgc caatcacaaa
      721 aaatgttgct ttcgcacctg ccagttcaag aacatccaat atttttttag tgacttttgg
      781 atcaggtcca tcgtcaaatg taagtgatat accttcaggt tttcctgtat taactgactt
      841 tataaaatag ttccatttaa tctgatagga gcccaatgct gtaattgtca gataaaggaa
      901 aataagtgcc gccaataaat aaaaaaagga tttgtcctga taaaggaaaa caaataatgc
      961 aataatgcag agaataaaaa ttacagttac gattttatgc ttcattgctt tttatcatta
     1021 tgaatccggc ccgtgcaggt agcaaggtgt ttattattaa agcccgttta tggttagcaa
     1081 ttgttttgcc ataaacttgt tttgtcttat tgcagattat atcagttgcc agatgcattg
     1141 caaaggcaga atttgtagca tacaatccgc aaatttcaga ataattaatg acgcacttat
     1201 ctggaaataa tgtttcaatt aatcttgtta tatctttatt acgtccatca ttcaaaataa
     1261 agtctatttg tttatctgtt gatgtgctca tgaagtcttt aatttcattt tcaagtgatg
     1321 aataaaaagc agaagtatga catgcttcaa taacagctaa agttttttcg tttttatttt
     1381 ttgataagac aaaaaatgaa gcaccacttg taagtggtaa cgagtttaat ttaaaatgtg
     1441 ctgcaatagg atgtaatgct tcaatatcct cgtctgcact gccaataaga atatttccat
     1501 ttttttcatc aagcattagc atagtatcga gcaacgcatg ttcaaacgac agactgtttt
     1561 gagtgtgtgt catattgtat tgatggttgc caagaaccaa tgaaatctga cctgcaatag
     1621 tgttgtgagt agattgaatg aatgaagtag gagggagcag cccgccttca atggtaatta
     1681 cattgtttag aaatttttca gtgtcataaa gacaacccaa tcctgtaccg gttattattg
     1741 catcaggttg ttcaatgcca gcttctttta atgcaagatt actgcaggca acagacattc
     1801 tcagtatttc actcatccgt ctgattaatg caggcggaac atactctttg taatcaggtt
     1861 taattaatga ttcaatttct ttagaaagat tcgaggaaaa ccctttgctt gcaaacgaag
     1921 gttgatgaga aatggtggaa gcagattgaa tacaatacat aatcaaatgc ctgaaaaaat
     1981 tagtgaagaa ttatttccgc caaaaccaaa agagtttgac atcacatgtt ttgttacagc
     2041 ttttgttggt tctgtaacag gatgcatgag tgtttcctcc aaagggtcat tgtagtttaa
     2101 tcctgcaaag tggatttgat tttttattgc aagaacagaa tatactgctt caattgcacc
     2161 ggctgctgcc aatgtatgac ctgtgtatgc cttggttgaa ctaaaaggtg gtatagactc
     2221 gccaaaaaca tttttaattg caatggattc gctaagatca ttgtttttag tccctgtgcc
     2281 atgagcgttg atgtaagaaa tttctttagg cccaagccct gctttcttca atgcattttt
     2341 cattgcaatt gtggctcctt taccatcggg tgatgaagca gtctgatgat aggcatcggc
     2401 agcattatgc catcctgtta aactgcaaat tggttcattg cccgaaatgt ttatgctgtg
     2461 atcactttcc aacaataaaa aacctgcgcc ttcacctagg ttaagtccgt ttctgttatg
     2521 atcaaatggc ttgcaccatt catcatcata tatcatcaac gattgaaagc ctttaattgt
     2581 aaaatctgtt atcggatcag ttccaccaac taaaactctg tccatttttc cttgctctat
     2641 cattcgtgct gcaagcatta tggcattagc ggctgatgaa catgctgtag aaagtgtatt
     2701 aatgtaacct gaaaatccta agtaagctgc aatattttc
//
LOCUS       DAORQT010000218         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7438_length_2738_cov_5.515840, whole genome shotgun sequence.
ACCESSION   DAORQT010000218 DAORQT010000000
VERSION     DAORQT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7438_length_2738_cov_5.515840"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1019)
                     /locus_tag="PK134_07180"
     CDS             complement(<1..>1019)
                     /locus_tag="PK134_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRB38666.1"
                     /translation="YNGPEGFSGNTFSAAAPADNFSLPNVSTFATAPIPSADVRMVNA
                     NYDGHFHTFNVEFNGNTIFTDNTIFGYGRHDYNFNIPAASLPLSNSLVFSGVANSGGT
                     NLMSVTYFKLQYPHANSFNGELEPFQFFSVSNGGSKARVDFTDFLNSDNSTRFIYIIA
                     GDTVSKVTTVRTGNLLQALIPVNGGEKNCLLAPASAYYFSTSDFYLSPENSDPDPQKF
                     ARFTNYSYNQPQYDYLIVSHKKLWSEAVNYRDFRAQTGFNPLLVDIDELYDQFAYGIY
                     KHPLAIKNFCRFSITAFNNKPDYLLLLGKAVYPEEVRVSPTADQLCLCRTRKSFFEKE
                     NKMFS"
     assembly_gap    1020..1055
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1066..1599)
                     /locus_tag="PK134_07185"
     CDS             complement(1066..1599)
                     /locus_tag="PK134_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38667.1"
                     /translation="MRKLYVIIVLFLLPVLSFAQFSNSWINFNGASPYSAQQYFKIKV
                     WKQGIYRIDYSLLQQAGFPVGMNPNQFQIFNNGHEQYIYVEGAQDNSFDPTDFIEFYG
                     KPNNGAADQELYDDPSDQLNPDYSLFCDTAVYFLTAAILPGTPVKRMIMETDVNFSLY
                     MPENYVTKTFRRNYTSK"
     gene            complement(1596..2669)
                     /locus_tag="PK134_07190"
     CDS             complement(1596..2669)
                     /locus_tag="PK134_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRB38668.1"
                     /translation="MKIVTLTWCCLILSIASVAQTAPKYSNEFLNLGVGGRALGMGGA
                     QVSQVNDVTAGYWNPAGLVHSRGDIQVSFMHNEYFAGIAKYDYLALTAPIDTMRRIGF
                     SVLRFGIDDIANTIDLKDNDGNIDYNRVTGFSAADYAFLVSYASKSAISGLDYGANFK
                     IIHRKVGDFAKAWGFGLDAGIQYQAGNWRTGAMLKDVTSTFNAWSYTLNDRTKEVFIQ
                     TGNEIPENGLELTLPRLILGVSRQFNIKKFTITPALDLETTFDGKRNTLVKTNFASID
                     PRVGIEGGYNNLVYVRAGVSNFQQVTDIDGKKTTTVQPNIGVGIKLRNLSIDYALTNI
                     GNSNYLYSNVFSLRLTVNKLKKT"
BASE COUNT          869 a          544 c          479 g          810 t
ORIGIN      
        1 tactaaacat tttattttct ttttcaaaaa aggatttccg ggtacggcac aaacaaagtt
       61 gatcggctgt tggacttact ctaacctctt caggatatac tgccttacct aacaacaata
      121 aataatcagg tttattatta aatgcagtga tagaaaaacg gcagaagttt tttattgcta
      181 acggatgctt gtaaattcca taagcaaact gatcgtacaa ttcatcaata tcaacaagca
      241 aaggattaaa tcctgtctgt gctctgaagt cacgataatt cactgcttcg ctccatagtt
      301 ttttatgcga gacaattaaa taatcgtact gcggctgatt gtaactgtag ttggtaaaac
      361 gtgcaaactt ctgtggatcg ggatcactat tttcgggaga caaataaaaa tcggaagtag
      421 aaaaatagta tgctgatgcc ggagcaagca ggcaattctt ttctccacca ttaacgggaa
      481 ttaaagcctg aagtaaattt cctgttctaa ctgttgttac tttagaaaca gtatcacctg
      541 caattatata aatgaaacgt gttgaattgt cgctgttcag aaaatctgtg aaatctactc
      601 ttgctttact accaccattg ctgacggaga aaaactgaaa aggttccaat tctccgttaa
      661 aagaatttgc atgaggatac tgaagtttaa aataggtaac agacataagg tttgttccac
      721 ctgaatttgc tacgccacta aacaccaaac tgtttgaaag tggtaatgat gctgcaggta
      781 tgttaaaatt atagtcatgt cgcccgtagc caaaaattgt attatcagta aaaatagtgt
      841 tgccattgaa ctcaacatta aatgtatgaa aatggccatc atagttggca ttaaccattc
      901 taacatccgc actggggatt ggcgcagtag caaatgtaga aacattggga agtgaaaaat
      961 tatctgctgg tgcagctgca gaaaaggtgt tacccgaaaa tccttcagga ccattgtatn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacct ttcagctatt ttgaggtata
     1081 gtttcttctg aaagttttgg taacataatt ttccggcatg taaagagaga aatttacatc
     1141 agtctccata atcattcgtt tcaccggtgt gccgggtaat atagctgctg taagaaagta
     1201 aactgccgta tcacaaaaca aactatagtc gggattcaac tggtcagacg gatcatcata
     1261 caactcctga tcggctgcac cattatttgg cttaccataa aactcaataa aatctgttgg
     1321 gtcgaatgaa ttatcctgag caccttcaac ataaatatat tgctcatgac cgttattgaa
     1381 aatctgaaac tggttagggt tcattccaac aggaaaacct gcctgctgca acaagctgta
     1441 atctatccgg taaataccct gcttccaaac tttaatttta aaatactgtt gtgcagaata
     1501 aggtgatgct ccgttaaaat taatccaact gttcgaaaac tgagcgaaac ttaaaacagg
     1561 aagaagaaat aaaacaatta ttacgtaaag ttttctcatg tctttttcag tttgttgacc
     1621 gtcaatcgca atgaaaatac atttgaataa agataattac tatttccaat gttagttagt
     1681 gcataatcaa tagacagatt acgcaacttt attccaacac caatattcgg ctgaacagtt
     1741 gttgtcttct ttccatcaat gtcagttacc tgctgaaagt tactcacgcc tgcacgcaca
     1801 taaacaagat tattataacc gccttcaata ccaactcttg ggtctatgct tgcaaaattt
     1861 gtcttcacca atgtatttct tttaccatcg aaggttgtct ctaagtctaa tgcaggtgtg
     1921 atggtaaact tcttaatatt aaactgtcgt gaaacaccaa gaattaatcg tggtaatgtc
     1981 agttccaaac cattttccgg aatttcattg cctgtttgaa taaacacttc tttggtacga
     2041 tcgttcaaag tatagctcca tgcattaaag gttgaagtaa catctttcaa cattgcacct
     2101 gtgcgccaat tgcctgcctg atattgaatg cctgcatcaa gtccaaaacc ccaggccttt
     2161 gcgaagtcgc caactttgcg atgtataatt ttaaaattag ctccgtaatc taatccgcta
     2221 atggcactct tactggcata agaaaccaga aatgcatagt cggcagcaga aaatcctgta
     2281 acacgattgt agtcaatatt tccatcgtta tctttcagat caatggtgtt ggcaatatcg
     2341 tcaatgccaa aacgaagtac agagaatcca atgcgtctca tagtgtcaat tggtgctgtc
     2401 aacgccaagt aatcgtactt ggcaatgcct gcaaagtatt cattatgcat aaaagatacc
     2461 tgaatgtcgc cacgagaatg caccaatcct gcaggattcc aatagcctgc tgttacatca
     2521 tttacctgcg aaacctgcgc accgcccatg cccaatgccc tgcctccaac gccaagattt
     2581 aaaaattcat tactgtactt aggggcagtt tgcgctacgg aagcaatact taaaatcaaa
     2641 caacaccatg tgagggttac tattttcata aactgatgat ttggatacct ataccaaacg
     2701 ataaattact aattttattt ttttctttgc caaacatg
//
LOCUS       DAORQT010000219         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7440_length_2738_cov_3.882221, whole genome shotgun sequence.
ACCESSION   DAORQT010000219 DAORQT010000000
VERSION     DAORQT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7440_length_2738_cov_3.882221"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..>607
                     /locus_tag="PK134_07195"
     CDS             228..>607
                     /locus_tag="PK134_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38669.1"
                     /translation="MEFSEIKLLINFFAKHRHDFLGVPDVYFADKNYPELLWFYKEIS
                     KGSINNDQEAAEKLLQSTATNPAYQQLKAELEDRLVNLVFGLDPEKLMNSMLGRSSFR
                     AYIYFGAAMILRQQNASAFFSDHF"
     assembly_gap    608..666
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <667..1277
                     /locus_tag="PK134_07200"
     CDS             <667..1277
                     /locus_tag="PK134_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38670.1"
                     /translation="AIKNKPDDFYHYRNRFKKSIKQFELEAELILMQRELDIFQVASN
                     ISQYKNAAKAKRYYLRAKKIHQQLKTFNSNFYYGNIAFIYGYFSSNFELVEEALNERE
                     AFYQNHPEYYSDFQKSFIALSRMLAYIHLRQYEKGKEEARIFLGKLSANSSDWLHALE
                     HYFILCMHSGNFEQALNIYYEATRSEYFYQLQSGSKRALALF"
     gene            1222..1710
                     /locus_tag="PK134_07205"
     CDS             1222..1710
                     /locus_tag="PK134_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38671.1"
                     /translation="MNTFINSNQEVKERWHYFEPYLNFILPGHFPKESINLLGFLDEI
                     SYYTTHKEDNNIIIMIGQIIVMIEMGDYDKLYDRTSYLESYIKKYVDKRIYPRTYIFL
                     NMLLTLFKNNFQKTKTQKQTAVSLEKLKPVAGNKFFNTEGLEVIPYEKLWAAISEKLK
                     RH"
     gene            complement(1754..1930)
                     /locus_tag="PK134_07210"
     CDS             complement(1754..1930)
                     /locus_tag="PK134_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38672.1"
                     /translation="MDLLISILLYLGVYATPADLQNKEFVAKNSEQINKAEMLSKDPN
                     FKCDGIVIVGSGNL"
     assembly_gap    2289..2310
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2513..>2738)
                     /locus_tag="PK134_07215"
     CDS             complement(2513..>2738)
                     /locus_tag="PK134_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38673.1"
                     /translation="LQIQNKKNMDLLLAILISMGINVTPKDLTNKEFMLKNQSAIQHA
                     QAILKERQTESTRITFKAAPQTNIFKQSKN"
BASE COUNT          881 a          416 c          478 g          882 t
ORIGIN      
        1 ctcataagag tgcggttgat gattaacgga gaagaagttt ccacagggat ggattttgta
       61 aagaaaaaag cagagcaaat tgcagcagaa agagcctgca ccaagcttga aatataatgc
      121 aggctgttaa caagccatct gaaaaataaa atttcaacgt tgcagtacat catttattga
      181 tgacctatat ttgaaagaaa ataaagcatt gatttatcgg gtataagatg gagttttctg
      241 aaatcaaatt attaattaat ttttttgcta aacaccgtca tgattttctt ggtgtgcctg
      301 atgtttattt tgctgataaa aattatcctg agctgctttg gttttataaa gagatttcga
      361 aaggcagcat caacaacgat caggaagctg ccgaaaaact attacaaagc acagccacaa
      421 atcctgccta tcagcaactg aaagctgaac ttgaagatcg tttggtaaat ctggtttttg
      481 ggttagatcc cgaaaaactg atgaactcca tgttggggcg aagttctttt cgtgcataca
      541 tttattttgg tgctgccatg atattacgtc agcagaatgc ctccgctttt ttttctgacc
      601 atttcttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnntggc tattaaaaat aaacctgacg atttttatca ttacagaaac cgctttaaaa
      721 aatctatcaa acagtttgaa ttagaagccg agttgatatt gatgcagaga gaactggata
      781 tttttcaggt agcttcaaat atttcacagt ataaaaatgc tgccaaggct aaacgatatt
      841 atctgagagc taaaaaaata catcagcaac ttaaaacatt caattcaaat ttttattacg
      901 gcaacattgc ttttatttat ggctatttca gcagtaattt tgaattggta gaagaagcct
      961 tgaatgaacg cgaagcgttt tatcagaatc atcccgaata ctattccgac tttcagaaaa
     1021 gttttattgc gctttcaaga atgttggcct acatccacct caggcagtac gaaaaaggta
     1081 aagaagaagc acggattttt ttaggtaaac tcagtgccaa ctcgtctgac tggctgcatg
     1141 cgctcgagca ttactttatt ctttgtatgc actcaggcaa ttttgaacag gcattaaaca
     1201 tttattacga agcaacacgc agtgaatact tttatcaact ccaatcagga agtaaaagag
     1261 cgttggcatt attttgaacc ttaccttaat tttatcttac ccggtcattt tccaaaagaa
     1321 agcatcaact tgttgggctt tttggatgaa ataagctact acaccacaca taaagaagac
     1381 aataacatta tcatcatgat tggtcagata atagtgatga ttgaaatggg cgactatgac
     1441 aaactttatg acagaacaag ttatctggaa tcgtatatta aaaaatatgt agataagaga
     1501 atttatccac gtacatatat ttttctgaac atgttgctca ccttatttaa aaataacttt
     1561 caaaaaacta agacacagaa acaaactgct gtatcgttag aaaaattaaa acctgttgcc
     1621 ggcaataaat tttttaatac tgaagggctt gaagtaattc cttatgaaaa actctgggca
     1681 gccatttcag aaaaattaaa aaggcactga ccatttaaag tcagcgcctt tgatattctt
     1741 ttggagacgt taattacaaa ttaccgctgc cgacaattac aattccatca catttaaagt
     1801 ttggatcttt tgaaagcatt tctgctttgt tgatttgttc tgaattttta gcaacaaact
     1861 ctttgttttg aagatcagcc ggtgtagcat aaacacctaa gtacagtaaa attgagatta
     1921 ataagtccat attgtttttt atttgaattt gtaagggtaa ttgcaaacca attgccgcta
     1981 ctcagttttt tctgacctcg aaatgggctt tctttctttt gtaagaatga tgaactaact
     2041 gtaagagttt atttcctgtt ttcagaggag aaattttctt taagttgctg ttaaacctga
     2101 aaaatcttat gtaagaaata ctaagagatg ttacttaaca gatgaaaaaa taataatgaa
     2161 gggaacttta aaatttattg ttgttttata ttaaaatggg gtagaaaaac gtagcagttg
     2221 tgttaactta aataaaaggc tcttccatat tctaaatcag tggggattct gattggatat
     2281 aaaatcagnn nnnnnnnnnn nnnnnnnnnn atgctatact tttgatgtag agaaggattc
     2341 tgaagtgctg ttgaaataaa atttaaatcc atttcgggtc ttatcaaatt tgcatcgtgg
     2401 atttcggtta taaattgttt ggataatttt caacatgcac actaatattt taaacaaaaa
     2461 agccgcctca gaaactgagg cggctatata tcagtgaaag atgtttcgga aattaatttt
     2521 tagattgttt gaaaatattt gtttgtggcg cagccttaaa agttatgcgt gttgactctg
     2581 tctgacgctc ttttaaaatt gcctgtgcat gttgtattgc actttgattc ttgagcataa
     2641 actctttgtt agttaaatct tttggggtta cattgattcc cattgaaatt aagattgcaa
     2701 gtaataagtc catatttttt ttgttttgaa tttgtagg
//
LOCUS       DAORQT010000220         2736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7450_length_2736_cov_5.457292, whole genome shotgun sequence.
ACCESSION   DAORQT010000220 DAORQT010000000
VERSION     DAORQT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7450_length_2736_cov_5.457292"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /gene="nadC"
                     /locus_tag="PK134_07220"
     CDS             <1..505
                     /gene="nadC"
                     /locus_tag="PK134_07220"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRB38674.1"
                     /translation="LYAEQVKHTKAKILDTRKTTPLNRYIEKWAVRIGGGHNHRFGLY
                     DMIMIKDNHVDYAGGIKQAIESANTFRKNRNLNIPIEIETRNLNEVEQVLNTGCVERI
                     MLDNFQPALIKEAVSMISGRFETEASGGIILQNVKDYAETGVDYISVGALTHSFKSMD
                     MSLKASQ"
     gene            514..1422
                     /locus_tag="PK134_07225"
     CDS             514..1422
                     /locus_tag="PK134_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRB38675.1"
                     /translation="MLEYLSLLLNYTRRITLPGFQKIPIYDVGEFFLRELFKGDLQIR
                     ARAIAFSFFLAIFPAIIFLFTLIPYVPIAGFQERLLSLLQSMLPYNTYDTAYKTIEEI
                     IVRQNGGLLSFGFILALIVSTNGVFALIEGFNKTIMHEKRKGWKQRLVALYLTVLLAT
                     LLIIAILLIIITEVILHYFNNHILNLDQSSVYLLLAGKYLILILLCFTAISSLYFFGP
                     SGGSKKWHFFSPGSILATILIAVTSIAFNFFILNFGQYNKIYGSIGTLIVILIYLNFN
                     CIQLLIGFELNNAIEKAKFKPTVVEK"
     gene            complement(1426..>2736)
                     /gene="lon"
                     /locus_tag="PK134_07230"
     CDS             complement(1426..>2736)
                     /gene="lon"
                     /locus_tag="PK134_07230"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRB38676.1"
                     /translation="PGVGKTSLGKSVAKALGRKYVRVSLGGLRDEAEIRGHRKTYIGA
                     MPGRIIQNIKKAKSSNPVFVLDEIDKLTNDFHGDPSSALLEVLDPEQNNAFYDNYIEL
                     DYDLSNVLFIATANSLSSIQPALRDRMEIIEINGYTVEEKIQIAVKHLIPKQLEAHGL
                     KKHHATFNNSVIEYIIEFYTSESGVRSLERNLASVIRSIAKRVALNEKYDKKIDKDTV
                     EKALGAPIFLRDRFSDNSVAGVVTGLAWTSVGGDILFIETSLSRGKGRLLLTGNLGEV
                     MKESAQIALAYLKAHAADFGIDYRVFDRWDIHIHVPEGATPKDGPSAGVTMLTSLASA
                     FTQRKVKLKLAMTGEITLRGKVLPVGGIKEKILAAKRAGITDIILCADNEKDIKDIKS
                     EYLKGLKFHYVSKMGEVIDIALLREKVKNPLNFTISQLERKETV"
BASE COUNT          833 a          542 c          470 g          891 t
ORIGIN      
        1 cctatatgca gaacaggtga aacatacaaa agcaaaaatt ttagatacca gaaaaacaac
       61 accactgaat cgttacatag aaaaatgggc tgttcgtatt ggcggtgggc acaatcatcg
      121 ctttggattg tatgacatga ttatgattaa agataatcat gttgattatg caggtggaat
      181 aaaacaagct attgaatcag caaatacttt cagaaaaaac agaaacctga atattcctat
      241 tgaaatagaa acacgcaact taaacgaagt tgaacaggtg ctaaacacag gttgcgttga
      301 aagaataatg ctcgacaact ttcaacctgc attaatcaaa gaagccgtgt ccatgatttc
      361 aggtcgtttt gaaacagaag cttccggtgg cattatactt cagaacgtaa aagattatgc
      421 tgaaacagga gttgactata tttctgttgg cgcattaaca cattctttta aaagtatgga
      481 catgagttta aaggcaagcc aatgaaacgc aggttgctcg aatacttatc actactgtta
      541 aattacacac gtagaattac gctacccggc tttcaaaaaa ttccaattta tgacgtgggt
      601 gaattttttc ttcgtgaatt atttaaaggc gatttacaaa taagagccag agcaattgcc
      661 ttttcatttt ttttagccat ctttcctgca atcatttttc tttttacact gattccttac
      721 gtaccgattg caggctttca ggaacgtctg ttaagtctgt tacaatctat gcttccctac
      781 aacacttatg acactgcata taagacaata gaagaaatta ttgtcagaca aaacggaggc
      841 ctgctttcat ttggttttat tctggcatta atagtttcta ccaatggtgt gtttgcattg
      901 atagaaggtt tcaacaaaac catcatgcac gaaaaacgta aaggatggaa acaaagatta
      961 gttgcattat atctaactgt tctgttagcg acattattaa tcatagcaat attactaatt
     1021 atcatcacag aagttattct gcattatttc aataatcaca ttctaaactt agatcagagc
     1081 tcagtttatt tgttgctcgc aggcaaatat cttattctga ttttgctttg tttcacagcc
     1141 atatcatcac tctacttttt tggtccttcc ggtggcagta aaaaatggca tttcttttca
     1201 cccggaagta ttctggcaac tatccttata gctgttacat ccattgcatt caactttttt
     1261 attctgaatt tcggacagta caataaaatt tatggttcta taggaacgct gatagtaatt
     1321 ctaatttatc tgaactttaa ctgcatacag ttgcttattg gatttgaact taataatgcc
     1381 attgaaaaag caaaattcaa gcccactgta gtagaaaaat aaatcttaaa cggtttcttt
     1441 tctttcgagc tgactgatag taaagttcaa tgggtttttc actttttcgc gaagcaatgc
     1501 aatgtcaatt acctcaccca ttttacttac gtaatgaaat ttcagtcctt taagatattc
     1561 gctcttgatg tctttaatat ctttctcatt gtcggcacaa agaattatgt ctgttattcc
     1621 tgcgcgcttt gctgcaagaa ttttttcttt aattccacca acaggcagta cttttccgcg
     1681 caaagtaatt tcaccggtca tagctaattt caacttaact ttacgttggg taaatgctga
     1741 tgcaagagaa gtgagcattg tgacacctgc agagggtcca tctttaggag ttgctccttc
     1801 aggaacgtga atgtgaatat cccatctatc aaacacgcga taatcaatgc caaaatctgc
     1861 tgcatgtgcc ttcaaataag ctaaagcaat ctgtgcagat tctttcatga cctctcccaa
     1921 atttcctgta agtaataagc gtcctttgcc tctgcttaaa ctggtttcaa taaataaaat
     1981 atcaccgcct actgatgtcc atgccaaacc tgtgacaact ccggcaacac tgttatctga
     2041 aaatctgtcg cgcaagaata taggggcacc aagtgctttc tctacagtat ctttatctat
     2101 cttcttatca tacttttcat ttaaggcaac tctctttgca atacttctga tgactgatgc
     2161 cagattgcgt tcaagactac gaacaccgct ttcgctggta taaaattcaa ttatgtattc
     2221 aattacactg ttattgaaag tagcatggtg tttctttaat ccatgtgctt ccaactgttt
     2281 tgggatgagg tgctttactg caatctgaat tttttcttca actgtgtagc cgttgatttc
     2341 aattatctcc atcctgtcgc gcagtgcagg ctgaatggag ctcaaactgt tggcagtagc
     2401 aataaacaac acattcgata agtcataatc aagctcaatg taattatcat aaaatgcatt
     2461 gttctgttca ggatctaaaa cttccagtaa tgcagaactt gggtcaccat gaaaatcgtt
     2521 ggtgagttta tcaatttcat caagaacaaa aacagggttt gatgattttg cctttttgat
     2581 attctgaata attctgccgg gcattgcacc gatataggtc ttacgatgtc ctcttatttc
     2641 tgcttcatca cgcaatccac cgagagaaac gcgtacatac tttctgccca aagccttggc
     2701 tacagattta ccaagagagg ttttaccaac tcccgg
//
LOCUS       DAORQT010000221         2731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7472_length_2731_cov_5.284006, whole genome shotgun sequence.
ACCESSION   DAORQT010000221 DAORQT010000000
VERSION     DAORQT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7472_length_2731_cov_5.284006"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK134_07235"
     CDS             <1..271
                     /locus_tag="PK134_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38677.1"
                     /translation="STTVSPYPVSFYPGVLCFTHSKLLLVEIDTVIVLSDVSFPVTTN
                     GAEKTPVTTHDPAGIGEVDASGLGTVVAPAGACPLMTGGRKPGKG"
     gene            complement(298..861)
                     /locus_tag="PK134_07240"
     CDS             complement(298..861)
                     /locus_tag="PK134_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38678.1"
                     /translation="MKNVLFILLAFVITTSCKKKDDDNEAQLIFKFRFDSTQQRQDNF
                     GNAATLPANHGALSPVFNSMSSHYIELAPTAFTQLGSGAVLYRNSETTQGGDNAIVFS
                     ESVLAGNNEEFFRVPFKNIAPGTYQYLRVSLAYQNYDITFGANILGTVYPNLKGTVAS
                     FIGFNSFINSFKIKDSTVTLNANRKQG"
     gene            complement(1287..1682)
                     /locus_tag="PK134_07245"
     CDS             complement(1287..1682)
                     /locus_tag="PK134_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38679.1"
                     /translation="MKELKMCPITKCEPGAKLIGVKIDNDVFDFLKENIMVDEDLFDV
                     MTAVSNPEKRFRFSNVCLETGCKHWTGAACGCVGKVLKNVEDLTLKKDAILPQCSIRE
                     RCRWFYQEGRMACDVCPLLAIKDKDLLEV"
     gene            complement(1909..2658)
                     /locus_tag="PK134_07250"
     CDS             complement(1909..2658)
                     /locus_tag="PK134_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRB38680.1"
                     /translation="MINLVLVDDEVNQINLIKDCVENYFPQIKILGTASSVPEAETLI
                     RSTKPDLVFSDIDMPPYTGFDLLQRFEKIDFGVVFITGFDKYAVRAFEVAAVDYLVKP
                     VTPEDLQRAIKKFEVEAERKRSAEMLQVLLHNHNISQKDEMHIALPKFSGFDVLKLKQ
                     VIHIRGEGNYSRFFLTDNTERLVTKQLGVYEKALTDFDFFRVHKSNMVNLAHVKSVSH
                     NNGGEVIMSNGNTIEIARGRKEDFLLRLKPI"
BASE COUNT          845 a          532 c          488 g          866 t
ORIGIN      
        1 atcaacaaca gtgtcgccat atccagtctc attctatccg ggggtgctgt gttttaccca
       61 ttcaaagctc ttgttagtag agatagatac tgtaatcgtt ttatctgatg tttcatttcc
      121 ggtaactaca aatggtgcag aaaagacacc tgtaaccaca catgaccctg cagggattgg
      181 cgaagttgat gccagcggat taggaacagt tgttgcaccg gcaggagcct gtccgctgat
      241 gacaggagga agaaaacccg ggaaaggata attttgattt aaggtttcaa atgcccatta
      301 accttgctta cgattagcat ttaatgtgac ggtgctgtcc tttattttaa atgagttaat
      361 aaaagagttg aatccaataa aagaagcaac agtaccttta aggttgggat aaacagttcc
      421 aagaatattg gcaccgaagg tgatgtcata attctgatac gctaatgaaa cacgtaagta
      481 ctgatatgtg ccgggagcaa tgtttttaaa cggcactctg aaaaactctt cattgttacc
      541 tgcaagtacc gactctgaaa aaacaatagc attgtcgcca ccttgtgtgg tttcactatt
      601 tctgtatagc actgccccac tacctaactg cgtaaatgcc gttggtgcaa gttcaatata
      661 gtgcgaactc atggaattaa atactggcga taatgcaccg tgattagcag gtagcgttgc
      721 tgcattgcca aaattatcct gacgctgttg tgtggagtca aaacgaaact taaatatcaa
      781 ctgcgcttcg ttatcatcat ctttcttctt gcatgatgta gttatgacga atgccaacag
      841 aataaataat acatttttca tttgtttgat ttttaaattc tatgctgcaa taataatcat
      901 atcctataat aggaaacgtt gtagttgatg aataattgta ctttagaagt gataaggtga
      961 ttatgcaatg aagtaataat ttaagtcctc tatacccgtt tagttgtatt ttggaattcc
     1021 aagaaaaata aaacaaataa gaatggcttt tacacttgat tgaagatgat aaatttgagt
     1081 taaatatact gcattttttt taaaaagcaa atatttttta ttcatttaca tcctaactcc
     1141 aaattcaaac tgcaaaatgt attgctgttt aagtttctgt caaaagaaac tttgcttcca
     1201 caatgaaact tacattcaag aaagtatttt gtcaattatc aattacacac ctatggaaat
     1261 atttaattac aaattgaaaa taatctttac acctccaaca aatctttgtc tttaattgca
     1321 agcagtgggc aaacatcgca ggccatacga ccttcctgat aaaaccaacg gcatctttcg
     1381 cgaatactgc attgcggtaa aatggcatct ttttttaagg tcaagtcttc aacattttta
     1441 agaaccttac ccacacagcc acaagctgct cctgtccagt gtttgcatcc agtttccaga
     1501 caaacattgc tgaaacgaaa tcgcttttca ggattgctga ctgcagtcat aacatcaaaa
     1561 aggtcttcgt ccaccatgat gttttctttt aaaaaatcaa atacatcatt atcaattttt
     1621 acacctatga gttttgctcc gggttcgcat ttggtgatgg ggcacatttt caactctttc
     1681 atgtctgtaa tttttgcggt tttataatat ttactttttt tcacttttaa aattttctga
     1741 ccccggcatt cgttaacacc gggaatcaga ttttttcaat tgggttagcc tcagggcgat
     1801 ttccaaatcc gagttgagga tatgacatca aagtaaaata caattaattt tacaacctaa
     1861 aggttttgta tcaaatgcag atgcggttgt acgagggaaa taaagctgct aaataggctt
     1921 caatctcaat aaaaaatctt cttttctccc tctggctatc tctatggtat tgccatttga
     1981 catgatgact tcaccaccat tattgtggct tacagatttt acgtgcgcta agtttaccat
     2041 atttgactta tgtacccgaa aaaagtcgaa atcggtgagt gctttttcat aaacacctaa
     2101 ttgtttagtg accaaacgct ctgtattgtc tgtcagaaaa aaacgggaat aattgccttc
     2161 gcctctgatg tgaataacct gctttaattt gagcacatca aagcctgaaa attttggaag
     2221 agcaatgtgc atttcgtcct tttggctgat gttatggttg tgcaataaaa cctgaagcat
     2281 ttcagccgac cttttacgtt ctgcctctac ctcaaacttt tttatggcac gctgaaggtc
     2341 ttcaggtgta acaggcttta ccaaataatc aacagccgct acttcaaatg cacggactgc
     2401 atatttatca aaacctgtta taaaaacaac accaaagtca atcttttcaa acctttgcaa
     2461 aaggtcaaat ccggtatagg gcggcatgtc aatatcactg aaaaccaaat cgggctttgt
     2521 gctgcgtatg agtgtttctg cctccggtac agagctggca gtgcccagaa tctttatctg
     2581 cggaaagtaa ttttctacac agtcttttat aagattaatc tggttgactt catcatcaac
     2641 tagcacaagg tttatcatgg cttaaaagta gcggaaatcc gacaaaaaaa ctcaggttgc
     2701 aaaaaatatt ttgcaacctg agtacctaac c
//
LOCUS       DAORQT010000222         2713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7538_length_2713_cov_6.649360, whole genome shotgun sequence.
ACCESSION   DAORQT010000222 DAORQT010000000
VERSION     DAORQT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7538_length_2713_cov_6.649360"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="PK134_07255"
     CDS             <1..1832
                     /locus_tag="PK134_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRB38681.1"
                     /translation="TVSNGSCSATSAPLVITVNPLPVATITASGSTTITQGQSVTLTA
                     SAGSSYLWSTGATTSSITVNTAGSYTVKVTNAQGCSATSSPMVITVVNNSQPASITTT
                     AANNQICMGQSATLTSSPGMQYIWLPGLQATQSIVVSAPGTYTVNIRNSDGSTSTANI
                     TITQAPTPNKPEVTYTYVAGTMYQLKAFEPSAASYLWNGGQTTPTISVNAAGVYSVVA
                     KNAYGCQSPAAKVQVNSLEPASCAKPDMLSAYNIIDKKASLMWNPAVTADSFRVAYNV
                     MGSSTVIVKYVRSRNNLDINELLPATTYQWKVTAICAGSSIVSATEVFTTLNGPTDCG
                     STVVNTFTTNVTNSSAQLNWYGTRATSITIRYKKLGASSYTYKSLATNNTTAWGFNLS
                     GLVSGTTYQWSVKTECGSVSSSYSSDNYFTTTSGCDVISGIAVSNVMSNRATVSWNPQ
                     SGVSYVVVKYAPATSSQWKYAYVRASAGQTIIRNLTPGVQYNIQLKSMCTYSNNSAYS
                     AMERFTTGIARLGEATSELMINAYPNPADAQITYAFDAKKEGYFFIRVSDIAGRILLN
                     DKKMASEGTNGGDIDLRMFTPGVYTLTITKGSEENHIRFEVNR"
     gene            1902..2657
                     /locus_tag="PK134_07260"
     CDS             1902..2657
                     /locus_tag="PK134_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38682.1"
                     /translation="MQMFAGSYYHCPMKYWSFKICAIAILCFFVDAIQAQTIHVVATV
                     KGNDATLPVVGLMIVNKSTGTGFFSPDNNFFSAVFNKSDTIMVNATGYSVKKICFKDS
                     LLRDTFRISIILEKPVVTLKPVTIIPPRELEVIQKDIEKLGYKKEDYMVTGIDAFESP
                     ITFLYQSFSRRERAKRDIAQKKNEDNKRKLLKELFRKYVDFDIMNLSEDQFDNFIDFI
                     NVDDNFLKNSTQYDFILFVKKKFEMYKALNRAK"
BASE COUNT          881 a          512 c          526 g          794 t
ORIGIN      
        1 taacagtttc taatggttct tgttcggcaa cgtcagcacc gttggtaatt actgttaatc
       61 cattgcctgt tgctacaata actgcaagtg gttcaactac catcacacaa ggacaatctg
      121 taactttaac tgcatctgcc ggtagcagct atctgtggtc aaccggtgca acaacttcat
      181 caattaccgt aaacactgcc ggaagttata ctgttaaggt tactaatgca cagggatgtt
      241 ctgcaacatc atcaccaatg gtgataactg ttgtaaacaa ttctcaacct gcatcaatta
      301 ccactactgc tgccaacaat cagatttgta tgggacaatc tgcaacactt acatctagtc
      361 ccggtatgca atacatctgg ttgcccggat tacaagctac acaaagtatt gttgttagtg
      421 caccaggtac atacactgta aacatcagaa acagtgatgg ctctacttca actgctaata
      481 tcaccatcac tcaggcacca accccaaata agcctgaagt tacctacact tatgttgcag
      541 gaactatgta tcagctaaaa gcttttgaac caagtgcagc atcgtatctg tggaatggtg
      601 gtcagacaac tccaacaatc tctgttaatg cagcaggtgt ttattctgtg gttgcaaaga
      661 atgcttatgg atgccagtcg cctgcagcta aagttcaggt taattcttta gagcctgcat
      721 catgtgcaaa acctgatatg ttgagtgcct ataacattat tgataaaaaa gcttctttga
      781 tgtggaatcc tgctgtaact gccgattcat tcagagtggc atataacgta atgggaagtt
      841 caactgtgat tgtaaaatat gtacgttcac gtaacaatct tgatataaac gaattgttac
      901 ccgcaactac ctatcagtgg aaagtaactg caatctgtgc aggaagttct atagtaagtg
      961 caacagaagt gtttactacg cttaatggac ctacagattg tggcagtacc gttgtaaata
     1021 ctttcactac caatgttaca aacagttctg cacaacttaa ctggtatgga actcgtgcaa
     1081 caagcatcac cattcgttac aaaaagttag gtgcatcaag ctacacgtat aaatcacttg
     1141 caaccaacaa cacaactgca tggggtttta atttatcagg tttagtttca ggaacaacat
     1201 atcagtggag tgtaaaaact gagtgtggta gcgtttcaag tagctacagc agcgataatt
     1261 attttactac tacaagcgga tgcgatgtga ttagtggaat tgctgtatca aatgtaatgt
     1321 caaacagagc tactgtaagt tggaatccac aatcaggtgt ttcttatgtt gtagtaaaat
     1381 acgcacctgc aacaagcagt cagtggaaat atgcctatgt aagagcatca gccggacaaa
     1441 caattatcag aaatcttact cccggagtac aatataatat tcagctaaaa tctatgtgca
     1501 cctattcgaa caattcagct tatagcgcaa tggagcgttt cacaacaggt attgcacgat
     1561 tgggagaagc tacttccgaa ctgatgataa atgcatatcc aaatcctgct gatgctcaaa
     1621 ttacttatgc ctttgatgca aagaaagaag gttatttctt tatcagagtg agtgatattg
     1681 caggacgaat tttgttgaac gataagaaga tggcttctga aggaaccaat ggtggcgaca
     1741 ttgatctgag aatgtttact cccggtgttt atacacttac tatcactaaa ggtagtgaag
     1801 agaatcatat aagatttgaa gtgaatcgct aaaaacagaa acagtaactt caaaatgaga
     1861 ggaggcttga aaaggcttcc tcttttttta taccaatctt catgcagatg tttgcaggaa
     1921 gttattatca ttgccctatg aagtattgga gttttaagat ttgtgccatt gccattcttt
     1981 gcttttttgt tgatgccata caggcgcaaa ccattcatgt agttgcaact gttaaaggca
     2041 atgatgcaac attacccgta gtaggattga tgattgtaaa caagtcaacc ggaactggct
     2101 ttttttctcc ggataacaat tttttttctg ctgtatttaa taagtcggac actataatgg
     2161 taaatgccac aggctattcc gttaagaaaa tatgttttaa agattcatta ctcagagaca
     2221 ctttcagaat atccattata ctcgaaaaac ctgttgttac actgaaacct gtaactataa
     2281 ttcctccacg cgaacttgaa gttattcaga aagacataga aaagttgggc tataaaaaag
     2341 aagattacat ggttacggga attgatgcat ttgaaagccc gattaccttt ttgtatcaga
     2401 gtttcagcag gagagagaga gccaaacgag acattgcaca aaagaaaaac gaagacaaca
     2461 agagaaaact tctcaaagaa ttgttcagga aatatgttga ctttgatatt atgaacctaa
     2521 gtgaggatca gtttgacaac tttattgact tcattaatgt tgacgataat tttctgaaaa
     2581 attctacgca gtatgatttt atacttttcg taaagaagaa attcgagatg tataaggctt
     2641 taaatagagc gaaataataa ctgtaactac ttctgcaaaa atcccggcag aatatctttt
     2701 gatgctgcag cca
//
LOCUS       DAORQT010000223         2712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7545_length_2712_cov_4.856605, whole genome shotgun sequence.
ACCESSION   DAORQT010000223 DAORQT010000000
VERSION     DAORQT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7545_length_2712_cov_4.856605"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK134_07265"
     CDS             complement(<1..185)
                     /locus_tag="PK134_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38683.1"
                     /translation="MSDQTQIMQKKELSFEDFRQVVLNDYRLGRISRETSLLGRREVL
                     TGKAKFGIFGDGKEVAQ"
     gene            324..920
                     /locus_tag="PK134_07270"
     CDS             324..920
                     /locus_tag="PK134_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38684.1"
                     /translation="MKTFLLASALFLSTSTFAQDKPELGNNIFSLTPMAIIGNESMDN
                     NTDVAVGLNYERILNEGLVSIKIPVNISLRNSGFFYIMPTIKLYPTRQGVVRYAVGPQ
                     FYIASGQINHHDYNTVWNGNQYITVNNKYSDNHTQLGFMINNSLNVSIAKTLYIGMEL
                     GLGIKYYDSNEENEYENSFDTTPVEPNVQFSFAMGYRF"
     gene            complement(1009..2388)
                     /locus_tag="PK134_07275"
     CDS             complement(1009..2388)
                     /locus_tag="PK134_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HRB38685.1"
                     /translation="MITVIIPTLNEEENIASVIKFAKQNSLVSEVLVVDDKSIDNTVR
                     IAQDNDARVITSTKIGKGASMREGVMFSKNEIIVFLDADINPYPHFTIKLLTDPIIHS
                     GAGFVKSSFNRNAGRVTELVAKPMLSIFFPDLLRFNQPLSGMIASKKSLLQQLDFRDD
                     YGVDIGLLIDMHLIHVNISEVEIGYIENKSKPWQALGKMSKEVAQTIIIKASSSGNPH
                     YNFEEFGVLSEIRSQMEFALNKQQETFNKLLIFDMDNTLLRGRFIDTCAERYGFKKQL
                     MDIRSSETDSTIITKRIAKLLKGRTIDELIAIADGISIVDDTEKIITKLKKRGYVVGI
                     ISDSYDCITNHVRNKLRMDFSLSNELEFSKSVCTGEVKIPSLFVNNESSSCKHVLCKT
                     NALQKLLEKYDIQKENSIAIGDSMNDLCMIKEAGLGIAFCSKDELLNHHADVIIRKPD
                     FSALLNIAK"
BASE COUNT          857 a          498 c          391 g          966 t
ORIGIN      
        1 atctgagcca cctcttttcc atcgccaaaa atgccaaact ttgccttgcc tgtcagcact
       61 tctctgcgtc ccaacaaact ggtttctcgg ctgatacgcc ctaaacgata gtcgtttagc
      121 accacctgtc tgaaatcttc aaatgaaagc tctttcttct gcattatttg ggtttggtct
      181 gacataattc cgctttttaa tagttacaaa ggtaacgaat gtactgaaat tctgaaagtg
      241 ccaaccatac aatttttgaa tttaaatgcc gtttttttga agtcttaaaa acactacttt
      301 agcttttaaa ataacataaa aatatgaaaa cctttctctt agcatctgca ctatttttat
      361 ctacatcaac atttgcccag gacaaacccg aattgggaaa taacattttt tctcttacac
      421 caatggctat tattggcaat gagtctatgg ataataatac cgatgtagct gtgggactta
      481 actatgagag aatactaaac gaaggattgg tgagtattaa aatccccgtt aacatttcat
      541 tacgtaattc gggatttttt tacatcatgc ccacgattaa actctaccct acaagacaag
      601 gtgtggtaag gtatgctgta gggccacagt tttatattgc cagcggacaa attaatcatc
      661 atgattacaa tactgtttgg aatggcaatc aatatatcac agttaataac aaatattcag
      721 acaatcatac acagttgggt tttatgatta ataacagcct gaatgtatca atcgctaaaa
      781 cgctatatat tggtatggaa ctgggattag gaattaaata ttatgattcc aacgaagaaa
      841 atgaatatga gaactcgttt gacactacac ctgtagaacc taatgtgcag tttagttttg
      901 cgatgggata tcggttttag tcttttcttt aaacactttg tcggtttcgg aagaagatag
      961 attaaagaat ttcaattatt ctatttatct aaatcaaaaa agacaaaatt attttgcaat
     1021 gttcagcaat gcactaaaat caggtttacg aattattaca tcagcatggt gatttaacaa
     1081 ttcatcttta gagcaaaaag ctattcccaa gccggcttct tttatcatac aaagatcatt
     1141 catgctgtct ccaatggcaa tgctattttc tttttgaata tcgtattttt caagtaattt
     1201 ttgtaatgca ttggttttac acaaaacatg tttacaacta ctgctttcat tattcacaaa
     1261 cagtgagggt attttaacct ctccagtgca aacacttttt gaaaactcta attcattaga
     1321 aagtgaaaaa tccattcgca atttatttct aacatgattg gttatacaat cataactgtc
     1381 agaaataatt ccaacaacat agccacgttt tttcaatttt gtgataatct tttctgtgtc
     1441 atcaacaatg gaaataccat ctgctatagc tatgagttca tctattgtcc ttccctttaa
     1501 caacttcgct attcttttgg taattatggt actatccgtt tctgaagaac gaatgtccat
     1561 tagctgcttt ttaaacccat acctttccgc acaggtatca ataaatctac cacgcagaag
     1621 tgtgttatcc atgtcaaata tcaataactt attgaaagtc tcctgttgct tatttaatgc
     1681 gaattccatt tgtgaacgaa tttcactcag cacaccaaac tcttcaaaat tataatgtgg
     1741 attacctgat gatgatgctt taataattat agtttgtgca acttctttac tcatttttcc
     1801 taaagcttgc cagggtttac ttttgttttc aatataacct atttctactt cactgatatt
     1861 tacatgtatc agatgcatat caatcagcaa tccaatatct acaccataat catcacgaaa
     1921 gtccaattgt tgaagcaagg attttttact ggcaatcatt ccgcttaatg gctgattgaa
     1981 tcttaataag tctggaaaaa agatgcttaa cattggtttt gctaccaatt ctgttactct
     2041 tcctgcattt cggttaaatg acgattttac aaatccggca ccactatgaa taattggatc
     2101 tgttaaaagt ttaatagtga aatgcggata agggttgata tctgcgtcta agaatacaat
     2161 aatttcattt tttgaaaaca tgacaccttc gcgcatagat gctccttttc ctatttttgt
     2221 actggtaatt acccttgcat cattatcctg tgcaatcctt acagtattat caatagactt
     2281 gtcatcaaca accaatactt ctgataccaa tgaattttgt tttgcaaatt ttataacact
     2341 tgcaatattc tcctcttcat taagtgtcgg tataataact gtaatcatct ttattaaaca
     2401 aattttttag tttttatgtt tatttttatt ttagttttat aataaaacta atctcatcta
     2461 tctgaacacg aatgtgccga tttggtattt gaaaaataca ctgaggaaat ctgatgtaaa
     2521 aataagtata atttaaaaac tatctgaata agttatatat ccgattaaat aattatactt
     2581 taccttcgcc cagtcaatct taatatatgc tttcaaaagt taaactgatt aaagtttttc
     2641 tacgcttatc ggcatttatt tgtttgtttt tcagctctga ctttgctgtt gcaaacaaag
     2701 ttgacacgct tt
//
LOCUS       DAORQT010000224         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7660_length_2685_cov_6.325095, whole genome shotgun sequence.
ACCESSION   DAORQT010000224 DAORQT010000000
VERSION     DAORQT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7660_length_2685_cov_6.325095"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PK134_07280"
     CDS             complement(<1..854)
                     /locus_tag="PK134_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38686.1"
                     /translation="MLFASCEKDNDKAIVLPPIDTTLLTMQVNIGVNYDTVVFVSLTS
                     GVVKSSAVKDFDLAFDAAPESKNIFLNTGKYMFAWRTQCTDMMNTDTTGLNWLTDSDN
                     MLGDSTVFGNNMISNYNANPNIVVIDRGKFNHIGSDRYRKVQVVSIDSVSYTIRYSKL
                     NNTDYHEFVVMRDNAYSQVYFSFDNGGKMVNIAPLKNQWDMMFTRYIHTYWDEPLQFR
                     YYSVNGVITNRWNNTTCAILKKDSLPNYMPFENFNHNNISAYPFHNTGNIIGFEWKFF
                     NFNDNKYY"
     gene            complement(904..1758)
                     /locus_tag="PK134_07285"
     CDS             complement(904..1758)
                     /locus_tag="PK134_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38687.1"
                     /translation="MQSINHFLNYLSVPDYALHSEFTIPLIPSLNLKYDITNIQTVRL
                     SYSRGFRTPGIKELYLDFIDINHNVQGNSNINPESSDNFQIVYSLLPEIKKVKLNSEV
                     KLYYNKVKNLITLANYNNTSNLYTYINIGHFTSQGVEWSNTIEVRSFSATAGVSYTGT
                     SSFNESLVNDLSWYPQANGSVSYNYKPFGLTTSVYYKYNGKVPVYVLDSNGNSLKQTN
                     EDYQLLDVTVSKKLWKERVEIGGGLKNIFNVTDVRRFSAGEIHNGGSSSSSLLPGRLF
                     FLKLSVNI"
     gene            complement(1667..>2685)
                     /locus_tag="PK134_07290"
     CDS             complement(1667..>2685)
                     /locus_tag="PK134_07290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRB38688.1"
                     /translation="VADVLSKQLNMSTGYDQAFGSTLTMMGMDMKNIKVLIDGVPVIG
                     RMNGNIDLNQLNLAAVERIEVVEGPMSTAYGTDAIAGAINLITKPQSLKKTTAGASAY
                     YENIGTYNTDLNVATNFKHAGIKITAGRNFFEGWNLTDDRRVLEWKPKTQYFGTALLQ
                     YTFRKVKVAYQLGLFDEKITDKGPVTITPFSAYAYDNYFRTFRNNHSASYDWFLSGKN
                     KLNGHVAFSGYDRNKETCLKDMVALEESELTGPDFSDETKFYSWNIRNAFSHVDENKN
                     FNYQGGIDINIEKGTGKRINSTASMQEYAAFASAEYKPFSKLLIRPGLRFTFRIYNSF
                     NTIS"
BASE COUNT          812 a          501 c          464 g          908 t
ORIGIN      
        1 atataatatt tattgtcgtt aaaattgaaa aacttccatt caaaaccaat gatgtttcct
       61 gtgttatgaa atggatatgc tgaaatatta ttgtggttaa aattttcgaa tggcatgtaa
      121 ttaggtaacg aatctttctt taaaatagca caagttgtat tgttccatct gttggtaatt
      181 acaccattta cagagtaata tctgaattgc aatggttcgt cccaataggt atggatataa
      241 cgggtaaaca tcatatccca ctgatttttt agcggagcaa tgtttaccat tttaccacca
      301 ttatcaaacg aaaaataaac ctgcgaatag gcattgtctc tcattacaac aaactcatga
      361 taatcggtgt tgtttagttt actgtatcgg attgtataag aaacactgtc tatgctcact
      421 acctgaacct tacgatatcg gtcgctgccg atgtgattga atttacctct gtcaattact
      481 acaatatttg ggttcgcatt ataatttgaa atcatgttat tgccaaacac cgtgctgtca
      541 ccaagcatgt tgtcagaatc tgtgagccag ttaagtcctg ttgtatcagt attcatcata
      601 tcagtacatt gtgtgcgcca agcaaacatg tacttaccgg tgttcagaaa aatatttttc
      661 gactcaggtg ctgcatcaaa tgccaaatca aaatctttta cagcactgct tttaactact
      721 cccgatgtaa gactaacgaa tacaaccgta tcgtagttaa caccaatatt tacttgcatt
      781 gtcaacaatg tagtatcaat aggcggcaat actatggctt tatcattatc tttttcgcaa
      841 gaggcaaata acattgctgc aatcggtaaa atgaaaaaga ataagctgac tttgtttctc
      901 atgctaaata ttgactgaaa gttttaagaa aaataatcgt ccgggaagta aagaggatga
      961 tgatgagcca ccattatgaa tttcgcctgc agaaaacctt cttacatcgg taacattaaa
     1021 tatgttttta agtcctcctc caatttcgac tctttctttc caaagttttt ttgaaactgt
     1081 tacatcaagc agttgataat cttcgttggt ttgtttgagg ctgttgccat tgctgtctaa
     1141 cacataaacc ggaacttttc cattgtactt ataataaaca cttgtagtca gtccaaaagg
     1201 tttatagtta taactcacag aaccatttgc ctgtggatac cacgataaat cattaactaa
     1261 cgattcgttg aaagatgatg tacccgtata ggaaacaccg gcagtagcag agaaacttct
     1321 tacctcaata gtattcgacc attcaactcc ctgcgaagta aaatgcccga tattgatgta
     1381 ggtgtagaga ttggaagtgt tgttgtagtt tgctaatgta atcaggtttt taactttgtt
     1441 ataatacaac tttacctcag agtttaattt cactttttta atttcaggca ataaagaata
     1501 tactatctga aaattatctg acgactcagg gttaatgtta ctatttcctt gcacattatg
     1561 attgatgtca ataaaatcga gatacagttc tttaattccg ggagtacgaa aaccacggct
     1621 gtatgaaaga cgtaccgtct gaatgtttgt tatatcatat ttcagattaa gagatggtat
     1681 taaaggaatt gtaaattctg aatgtaaagc gtaatccggg acggataagt agtttagaaa
     1741 atggtttata ctctgcactt gcaaaagctg catattcctg catggatgct gtgctgttga
     1801 tgcgcttgcc cgttcctttt tcaatattaa tatctatgcc tccctgataa ttgaaatttt
     1861 tattttcatc aacatgactg aatgcatttc tgatgttcca gctgtaaaat tttgtttcat
     1921 cactaaaatc agggcctgtt aattctgatt cttcaagcgc gaccatgtct ttgagacaag
     1981 tttctttatt tctgtcatag ccggaaaaag caacgtggcc gttcaattta ttttttccgg
     2041 aaagaaacca atcgtagctt gctgagtgat tgttgcgaaa tgttctaaaa taattatcat
     2101 aggcataggc agaaaatggt gtgatggtta ccggaccttt atcagtaatt ttttcatcaa
     2161 ataaacccaa ctgataagca accttcactt ttctgaaagt atattgcagc aatgctgtac
     2221 caaaatattg tgtcttgggt ttccattcaa gcacccttct gtcatctgtt aaattccatc
     2281 cttcaaaaaa gtttcttccg gcagttattt ttattcctgc atgtttaaag ttagttgcaa
     2341 cattcaaatc ggtattgtag gttccaatat tttcataata tgcagaagca cctgcagtag
     2401 tttttttaag cgactgcggt ttggttatca gatttatagc gcctgcaatg gcatcggtac
     2461 cataggcagt actcattggc ccttctacca cttctattct ttcaacagca gcaaggttta
     2521 actgattcag gtctatattt ccattcattc ttccaataac aggaacacca tcaatcaaca
     2581 ctttaatatt tttcatatcc atgcccatca tggttaatgt actgccgaaa gcctgatcat
     2641 aacctgtgct catgttgagt tgtttgctga gaacatcggc aacat
//
LOCUS       DAORQT010000225         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7793_length_2649_cov_5.013878, whole genome shotgun sequence.
ACCESSION   DAORQT010000225 DAORQT010000000
VERSION     DAORQT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7793_length_2649_cov_5.013878"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK134_07295"
     CDS             <1..389
                     /locus_tag="PK134_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB38689.1"
                     /translation="GWRMRVMLARMLLKKPSLLMLDEPTNHLDLPSIQWLEKYLQDYD
                     GTFILVSHDRYFLDRTINMIAEVANEKINLYPGNFTNYLEQKTMRDELQQRKFENQEK
                     YIKEQQKLIDRFKAKASKAKMAQSRM"
     gene            390..1016
                     /locus_tag="PK134_07300"
     CDS             390..1016
                     /locus_tag="PK134_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB38690.1"
                     /translation="MLDRMEKVEAVSGPASTMRLSLQVETQPGKVVFEGHHLNKNYGD
                     LDIFKNSHFTIERGDKIALIGANGKGKSTLLRMIAGTEKADGEIKEGYNVKTSFYAQH
                     QLESLNIENDLMRELQIFAPLETDQRIRSILGAFLFNRDDVFKKIRVLSGGEKARVAL
                     AKLILSKANFLLLDEPTNHLDMQSVEILTDVLLDYEGSFVGFTRSFFC"
     gene            1168..1422
                     /locus_tag="PK134_07305"
     CDS             1168..1422
                     /locus_tag="PK134_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38691.1"
                     /translation="MQAEPDQKKQLLNLEKEVKKLEAELAAAKKQRNDLMEQLGNESN
                     YNDTAKIKLLQKEFDESEKQYNKIHSLWEDSYMQWMEMSE"
     gene            1430..>2649
                     /locus_tag="PK134_07310"
     CDS             1430..>2649
                     /locus_tag="PK134_07310"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRB38692.1"
                     /translation="MKHFIFYLILFFLWSNSNVFGQEKQEYFKDNYFRYDNFTYRSNI
                     KTIQLENAQFPMSEPVIELNSGAQLLLQFDDLEGDYKDYWYTIIHCDAQWKPSDITRS
                     TYLFSFYEDRIADYAFSFNTLQPYTHYSLKFPNEQIKPLISGNYLLKVYLNNDPDSVA
                     FTRRFLVYENLIDVDATVHAATLAEYRQQKQEVDFSLSTTRYTIANPFGDLKVKILQN
                     FRWDNAVENLKPIFIKDNLLEYNYDEENTFNGSNEFRRFDIRSLKYETESVERIDHFD
                     TGTNVLLFPDKVRYFSRYTTDYDIDGKFEIKNYSGSHPERDADYATVRFRLEYKDEVK
                     DGNIYIFGALTDWGFTSQNMLRYDEDLKAYTATLFLKQGYYNYMYVFLKDGTSFGDES
                     WLEGNHYETENNYW"
BASE COUNT          900 a          440 c          549 g          760 t
ORIGIN      
        1 gaggatggcg tatgcgcgta atgcttgcac gaatgctgtt gaagaaacca agtctgttaa
       61 tgcttgatga gccaaccaat cacctcgatt taccttccat acaatggctt gaaaaatatc
      121 tgcaggatta tgatggtacc tttattcttg tttcgcacga cagatatttt cttgaccgca
      181 ctatcaatat gattgcggaa gttgctaatg aaaaaattaa tctttatccg ggtaatttca
      241 ccaactatct cgaacagaaa actatgcgtg atgaattgca gcagcgtaaa ttcgagaatc
      301 aggaaaagta tattaaagag caacagaaat taattgaccg ctttaaagca aaagccagca
      361 aggcaaagat ggctcagtcc agaatgtaaa tgctcgaccg tatggaaaaa gttgaagcag
      421 tttccggccc tgcatcaacc atgagattaa gtttacaagt agagacacag cccggcaagg
      481 tagtttttga aggacatcat ctgaataaaa attatggtga tctggatatt tttaaaaaca
      541 gtcattttac aattgaaaga ggtgataaaa ttgcacttat aggtgctaac ggaaaaggta
      601 agtcaacctt attgcgcatg attgccggca cagaaaaagc agatggcgaa atcaaggaag
      661 gctacaatgt aaagacatct ttctatgcac agcatcagtt agaaagtttg aatattgaga
      721 acgatttgat gcgcgaactg cagatttttg ctccgttaga aaccgatcaa cgtatcagaa
      781 gtattctggg tgcatttctt tttaatcgtg acgatgtttt taagaaaatt cgagtacttt
      841 caggaggtga gaaagcacgt gtggcattag cgaagttaat tctcagcaaa gcaaattttc
      901 tgttattaga tgagcctacc aaccacctcg acatgcaaag tgtagaaatt cttactgatg
      961 tgttgttgga ttacgaaggc tcttttgtag gtttcacacg atcgtttttt tgttgaacag
     1021 attgccaata aaatatggtt tatcgagaac aatcagctta aagaatatcc cggaacttat
     1081 gaagagtatg agcattgggc agaacgaaaa aaatctgaaa aacgaacaga agaaaaacaa
     1141 gttgtagcca aacctgttgc accaaaggtg caggcagagc ccgatcagaa aaaacagttg
     1201 ttgaatttag agaaggaggt taaaaaattg gaagcagagt tggcagcagc aaagaagcaa
     1261 cgcaatgatt tgatggagca gttgggcaat gaaagtaatt ataatgacac agctaaaatc
     1321 aaactgttac agaaggagtt tgatgaatct gaaaaacagt ataataagat acattcattg
     1381 tgggaagaca gctacatgca atggatggaa atgagtgaat gatgtgctta tgaaacattt
     1441 tattttttat ctgatacttt tctttttgtg gtctaactct aatgtgtttg gtcaggaaaa
     1501 gcaagaatat tttaaagaca attatttcag gtacgataac tttacgtaca gaagtaatat
     1561 taaaacgata caactggaaa atgcacagtt cccgatgagt gaacctgtga ttgaactcaa
     1621 cagtggagcg cagcttttac tccagtttga cgaccttgaa ggcgattata aagattactg
     1681 gtacaccatc attcattgcg atgcacaatg gaagccttca gacattacgc gctcaacata
     1741 tttgtttagc ttctacgaag accgtattgc tgattatgct ttttcgttta acaccttgca
     1801 accttacact cattactcat taaaatttcc aaacgaacag attaaaccac tgatatcagg
     1861 aaattatttg ctgaaagttt atttgaataa cgaccctgac agtgttgcat ttacaagaag
     1921 atttttagtg tatgaaaacc tgattgatgt tgacgctacc gtgcatgcag caacattggc
     1981 tgaatacaga caacagaaac aggaagttga tttttcattg tcaactacaa gatataccat
     2041 cgccaatccc tttggtgatt taaaagttaa aattcttcag aatttcagat gggataatgc
     2101 tgttgagaat ctgaagccga tttttattaa agataacttg ctggaatata attatgatga
     2161 agaaaatact tttaatggca gtaatgagtt cagacgcttt gatattcgct cattaaagta
     2221 tgaaacagaa agtgtagagc gaatagatca ttttgatacc ggaaccaatg tgttgctttt
     2281 ccctgataag gttcgttatt tcagtcgtta tacaaccgac tatgatattg acggaaagtt
     2341 tgaaataaaa aactacagtg gcagccatcc tgagcgtgat gccgattatg caaccgttcg
     2401 tttccgcctt gaatataaag acgaagttaa agatggcaac atttatattt ttggggctct
     2461 caccgactgg ggttttacga gtcagaacat gcttcgttat gatgaagacc tgaaagctta
     2521 cacagcaaca ttgtttttaa aacaaggcta ctacaattat atgtatgtgt ttttaaaaga
     2581 cggaacttct tttggagatg aaagctggct tgaaggcaat cactatgaaa cagaaaacaa
     2641 ctattggat
//
LOCUS       DAORQT010000226         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7954_length_2615_cov_6.719531, whole genome shotgun sequence.
ACCESSION   DAORQT010000226 DAORQT010000000
VERSION     DAORQT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7954_length_2615_cov_6.719531"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>697)
                     /locus_tag="PK134_07315"
     CDS             complement(165..>697)
                     /locus_tag="PK134_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HRB38693.1"
                     /translation="GALYKSFETENAALTAFQKGLSGFQKKSESQHLFQVEKSSSKPI
                     KESIAVDGAWNTKTLESEYRGVDVKTGKQLFLKGPYADGTNNIMEFLAIVHALAYCKR
                     HHINLPIYSDSMTALKWVKTKKANTKLASTGRNEELFELIDRAEEWLKNNIYANKLLK
                     WETQIWGENPADFGRK"
     assembly_gap    698..729
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<730..792)
                     /locus_tag="PK134_07320"
     CDS             complement(<730..792)
                     /locus_tag="PK134_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HRB38694.1"
                     /translation="MAKKKFYVVWKGVMPGVYHSW"
     gene            complement(797..1363)
                     /locus_tag="PK134_07325"
     CDS             complement(797..1363)
                     /locus_tag="PK134_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRB38695.1"
                     /translation="MNIQIKEIITVSMILFSVIDIIGSVPVIIGIRKSVGTIHPEKTS
                     LVALMIMIVFLFVGENILSLIGLDVSSFAIAGSFILFFLALEMILGVKLYKDEMPASA
                     SIVPIAFPLIAGSGTMTTLLSLRSQYDMLTIIIGVLLNMIWVYLCLKNLGFIERLLGP
                     TGIAIVRKVFGFILLAIAVKLFRSNLGI"
     gene            complement(1393..>2615)
                     /gene="lpdA"
                     /locus_tag="PK134_07330"
     CDS             complement(1393..>2615)
                     /gene="lpdA"
                     /locus_tag="PK134_07330"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRB38696.1"
                     /translation="NAVNKFSEHGIKIKDIKADMEQMIKRKAQVVKQTCDGISFLMKK
                     NKVTVLTGHATFVDSKQLDVVDAGGKSNRVGATNFIIATGSKPSSLPGIEIDKKRIIT
                     STEALELKTIPGHLVVIGGGVIGLELGSVYARLGAKVTVIEYMNAIIPTMDRALGKEL
                     QKVLSKEGFTFLLGHKVQSVKSSAKQVEVVGESSSGEKVNIIGDYCLVSVGRKPYTEK
                     LGLDKAGVALDERGRIITDEKLQTNVPGIYAIGDVIKGAMLAHKAEEEGVFVAEQLAG
                     QQPHINYRLIPGVVYTWPEVASVGYTEEELKESGVKYKTGNFPFKASGRARASMDTDG
                     FVKVLADSTTDEILGVHIIGPRAADMIAEAVVAMEYRASAEDIARICHAHPTFTESFK
                     EACLAATANRAIHI"
BASE COUNT          759 a          558 c          400 g          866 t
ORIGIN      
        1 ttatatgaca gcaattaagt aacaatatga tataaatcag tttttgctta caacttattg
       61 tttttgaata cccccaaatg ctattcaagc tttttcagtt ctaaaaaaaa ctgtgtttgg
      121 ttgcataaat ttaaagggga acagaaaaaa gcatcaattt tcaactactt gcgcccaaaa
      181 tccgcaggat tttcacccca gatttgcgtt tcccacttga gcagtttgtt tgcataaata
      241 ttattcttca accattcttc tgcacggtca attaattcaa acaattcctc atttcgaccc
      301 gttgaagcta attttgtatt tgcttttttc gtctttaccc atttcaaagc tgtcatgcta
      361 tcggaataaa tgggtagatt aatgtgatgt cgtttgcaat aagccaaagc atgcacaata
      421 gccaaaaact ccataatgtt gtttgtgcca tcagcataag ggcctttaag aaaaagttgt
      481 ttacctgttt ttacatctac ccctctgtac tcactttcca atgttttagt attccatgca
      541 ccatcaactg caatactttc ttttattggt tttgatgacg atttttctac ttgaaataaa
      601 tgttgtgatt cacttttttt ctgaaacccc gacaatcctt tttggaatgc ggtaagtgct
      661 gcattttctg tttcaaaact tttatacaaa gctcctgnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnc cacgaatgat aaactccggg cattacccct ttccaaacaa catagaattt
      781 ttttttagcc atgagtttat attccgagat tacttctgaa aagttttact gcaatagcta
      841 gcaaaataaa accaaatact tttctaacga ttgctattcc tgttggccca agaagccttt
      901 caataaatcc aagattcttc agacagagat aaacccaaat catatttagt aatactccaa
      961 taataatggt aagcatatca tactgactgc gtaaagaaag tagtgtagtc atggtgccac
     1021 tacctgcaat aagtggaaat gcaattggaa caatacttgc cgatgccggc atttcatctt
     1081 tataaagctt tacaccaaga atcatttcta aggcaagaaa aaaaagtatg aatgagcctg
     1141 caatagcaaa agacgagaca tcaagtccta tcaatgacag aatattttct cctacaaaga
     1201 gaaaaacaat cataatcatc aatgctacca acgatgtttt ttcggggtga atggtaccta
     1261 cactttttct gataccgatt attacaggca cagagccaat gatgtctatt accgagaata
     1321 atatcattga aacggtaata atttctttta tctgaatatt cataaattat atgttagggg
     1381 tattatgttt aatcagatgt gtatggctct gtttgcagta gctgcaagac aagcttcttt
     1441 aaaactctct gtaaatgttg gatgtgcatg acaaatacgt gcaatgtctt ctgccgatgc
     1501 acgatactcc atagccacaa cagcttcggc aatcatgtct gccgcacgtg ggccaataat
     1561 atgtacacca agaatttcat cagtagtgct atcagccaac accttcacaa aaccatccgt
     1621 gtccatactt gctctggctc tgccactggc tttgaatgga aagtttcctg ttttatattt
     1681 cactcccgat tctttaagtt cttcttctgt gtaaccaaca gatgctactt caggccatgt
     1741 gtaaactact ccgggaataa gtctgtaatt gatgtgcggc tgttgacctg ccagttgttc
     1801 tgccacaaac acgccttctt cttctgcctt atgtgcaagc atagcacctt taattacatc
     1861 gccaatggcg taaatgccag gcacgttggt ttgtaatttt tcatccgtaa taattctacc
     1921 cctttcatca agtgcaacac ctgctttgtc aaggcctaat ttttctgtgt atggctttcg
     1981 acctactgaa accagacaat aatctcctat gatattaacc ttttcaccac tgctgctttc
     2041 tccaacgact tcaacttgtt tagctgatga ttttaccgat tgtactttat gcccaagtaa
     2101 aaaagtaaat ccttccttac tcaacacttt ctgtaattct tttcctaatg cacgatccat
     2161 tgttggaata atagcattca tatattcaat aacagtaacc tttgctccta atcgtgcata
     2221 aaccgagccc aattcaagac caattactcc tccaccaata actactaaat gaccgggaat
     2281 agtttttagc tctaacgctt ctgttgatgt gatgatacgt ttcttatcta tctctattcc
     2341 gggtaatgat gatggtttag accctgttgc tattataaaa tttgttgctc ctactctgtt
     2401 tgacttacca ccagcatcaa ctacatccaa ttgttttgaa tccacaaagg ttgcatgacc
     2461 tgtcaataca gttactttat tttttttcat cagaaagcta atgccatcgc aagtttgttt
     2521 cacaacctga gccttgcgct taatcatctg ctccatatcg gctttgatat ctttgatttt
     2581 tatgccatgc tcactgaatt tatttacagc attaa
//
LOCUS       DAORQT010000227         2614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7957_length_2614_cov_11.059398, whole genome shotgun sequence.
ACCESSION   DAORQT010000227 DAORQT010000000
VERSION     DAORQT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_2614_cov_11.059398"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="PK134_07335"
     CDS             <1..127
                     /locus_tag="PK134_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB38697.1"
                     /translation="KEGNFPVTEKLCANVLSLPMHTELDEEQLEYITRAVLEFCK"
     gene            158..1471
                     /locus_tag="PK134_07340"
     CDS             158..1471
                     /locus_tag="PK134_07340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRB38698.1"
                     /translation="MNIGVVGTGYVGLVTGTCFAEMGNHVVCVDIDQAKVDSMKQGKI
                     PIYEPHLDVLFERNIRQNRLSFTTQLTDAVKHSSIIFLALPTPPGEDGSADLSYVLGV
                     ADQLGKMLDGYKIIINKSTVPVGTAEAVRDAIAKNYKGEFDVISNPEFLREGFAVDDF
                     MKPDRVVIGTRSEKAKKIMGELYAPFLRQGNPIYFMDERSAELTKYAANSFLATKITF
                     MNEVANLCEKVGANVDAVRIGIGSDDRIGKRFLFAGIGYGGSCFPKDVQALEHTSGKY
                     GYDFSILQSVMSVNNKQKMLLAEKALEYYGGNVKGKRFALWGLAFKPDTDDIREAPSL
                     YIIDMLTKAGATIRAFDPEAMNNVKKLIGDKIEFANDEYHALEGADALLIATEWSAFR
                     TPDFDKMHSALKSKVIFDGRNLYEPQKMQEAGYYYNSIGRQTVKP"
     gene            1468..2439
                     /locus_tag="PK134_07345"
     CDS             1468..2439
                     /locus_tag="PK134_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB38699.1"
                     /translation="MTKRILITGAAGFLGSHLCDRFIQEGYQVVGMDNLITGDLNNIA
                     HLFKLREFDFYHHDVSKFVHVPGKIDYILHFASPASPIDYLKIPIQTLKVGSLGTHNL
                     LGFALAKKARILVASTSEVYGDPLVHPQNEDYWGNVNPIGPRGVYDEAKRFQEAITMA
                     YHTYHKLETRIVRIFNTYGPRMRLNDGRALPAFVGQALRGEDLTVFGDGSQSRSFCYV
                     DDLVEGIFRLLHCDYAQPVNIGNPVEITIKQFAEEIIKLTGTTQKIIYKPLPQDDPKQ
                     RQPDITRAREILGWEPKVSREDGLKITYEYFKSLPPEELKRSAHRFA"
BASE COUNT          826 a          452 c          553 g          783 t
ORIGIN      
        1 taaggaagga aattttccgg taacagaaaa actttgtgcc aatgtgctgt cattacccat
       61 gcatacagag ttggatgagg agcagttgga atatattacc cgtgcagtac ttgaattttg
      121 taaatagaaa ataattttat ccgtatagta ttttaatatg aacattggag ttgtaggtac
      181 aggttatgta ggtttggtta ccggaacctg ttttgccgaa atgggcaatc atgtagtttg
      241 tgtggacata gatcaggcaa aagttgatag catgaaacaa ggaaaaattc ctatctatga
      301 gcctcatctt gatgtgttgt tcgagaggaa tatcagacaa aatcgtttgt cgtttaccac
      361 gcagcttaca gatgcagtta aacattcatc aattatattt cttgcattac ccacgcctcc
      421 cggtgaagat ggctctgcag atttatctta tgtactgggt gttgccgacc aattaggaaa
      481 aatgctagat ggttataaaa tcatcatcaa taagagtaca gtgcctgttg gtacagcaga
      541 agcagtgaga gatgctattg ctaaaaacta caaaggcgaa tttgatgtca tctctaatcc
      601 tgaattttta cgcgaaggtt ttgcagtaga tgattttatg aaacccgatc gtgttgttat
      661 tggaacacgt tcagagaaag caaaaaaaat tatgggagaa ctgtatgcac cttttcttcg
      721 tcaaggcaat ccaatatatt ttatggacga gcgcagtgca gagttaacta agtatgcagc
      781 aaactcattt ctggcaacaa aaattacttt catgaatgag gttgccaatc tctgcgaaaa
      841 agtgggtgct aatgttgatg cagtgcgcat tggcataggc tctgatgaca gaatcggtaa
      901 gcggttttta tttgccggta ttggttatgg tggaagctgt ttcccaaaag atgtgcaggc
      961 attagaacac acatccggca agtatggata tgattttagt attctgcaat cggtgatgag
     1021 cgtaaacaac aagcaaaaaa tgttgcttgc agaaaaagca ttggaatatt atggtggtaa
     1081 tgtaaaagga aaacgatttg cattgtgggg acttgcattt aaacccgata ccgatgatat
     1141 tcgcgaagca ccatcacttt atattattga catgcttaca aaagcaggtg caaccattag
     1201 agcatttgat cctgaggcaa tgaataatgt gaaaaaactg ataggtgata aaattgagtt
     1261 tgctaacgat gagtatcatg cattagaagg tgctgatgct ttgttgatag ccacagaatg
     1321 gagtgccttc agaacacctg attttgacaa aatgcattca gctttgaaaa gcaaagtaat
     1381 ttttgacgga cgtaatttat acgaaccaca aaaaatgcag gaagcgggat attattataa
     1441 cagcattggc cgccaaacgg taaaaccatg acaaaaagaa ttttaattac cggtgcagcc
     1501 gggtttttag gatcacacct gtgtgatcgt tttattcagg aaggttatca ggttgttggt
     1561 atggacaacc taattacagg cgatttaaat aatatagcac atctctttaa gctgcgtgag
     1621 ttcgattttt atcatcacga tgtttctaag tttgtgcacg tgcccggtaa gattgattac
     1681 atattgcatt ttgcatcacc tgcatcacct atagattatc ttaaaattcc aattcaaaca
     1741 ctaaaggtag gttcattagg tacacataac ttacttgggt ttgcattagc aaagaaagca
     1801 cgcatattag tggcaagcac ttctgaagtt tatggagacc cattggttca tccgcaaaac
     1861 gaagactatt ggggtaatgt aaatccaatt ggcccgcgtg gtgtgtatga tgaagcaaag
     1921 cgttttcagg aagccattac gatggcctat cacacctatc ataaattaga aacacgtatt
     1981 gtacgaattt ttaataccta tggcccacgc atgagactta atgatggtcg tgcactgcct
     2041 gcatttgttg gtcaggcttt gcgtggcgaa gaccttaccg tttttggtga tggcagtcag
     2101 tcgcgttcat tttgttatgt ggacgatttg gtagaaggta tttttcgttt gcttcactgc
     2161 gactatgccc aacccgttaa cataggaaat ccggtagaga taactatcaa acaatttgcc
     2221 gaagaaatta ttaagcttac aggcacaaca caaaaaatta tttacaagcc actgccgcaa
     2281 gacgatccta agcaacgtca gcccgacata acacgtgcac gggaaatatt gggatgggaa
     2341 ccaaaagttt cgcgcgaaga tggattaaag attacttacg aatattttaa gtcactacca
     2401 ccggaagagt tgaaacgctc tgcacatagg tttgcttgat ttgtaatttt tcttttattc
     2461 caactagtat gagacatttt cttcttcaaa gattataatg ataaacaaat agaagaaaaa
     2521 atttatcatt tataaccaat cgaattatgt attttatttc aataactcac tttctttttg
     2581 ccttctaaca caaaacctag tgtaaaatca tttt
//
LOCUS       DAORQT010000228         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_7993_length_2607_cov_17.844044, whole genome shotgun sequence.
ACCESSION   DAORQT010000228 DAORQT010000000
VERSION     DAORQT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7993_length_2607_cov_17.844044"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1735
                     /locus_tag="PK134_07350"
     CDS             242..1735
                     /locus_tag="PK134_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38700.1"
                     /translation="MAFKRIFFFVWFSVCFHVAHAQKVTYAQQELDAYSYDYIKTAGY
                     NDNSFFVFQSNLPFENDRDRVGFKSRKYKVTCYNLNLSLKWSKKIEPKNDNEKLETIT
                     VIDGQVALIKSSFEGKDNQLSVFITTLDDNGNESATKKEIGTILFDRTSNLDKIKVIA
                     SKGNNYFGLIQNEKKQNGLQQLHCLIIDSDFKLVRQLQFEVPYPEKNFNYENWLLTDD
                     ADFILEGSYYSKEKSENRKRWDAYKLFVSSNGNSGAIEYAINTSNLILSSSTIAYDAI
                     NKKIVLAAFYTDNVSTKSGIIYASLGTSGNDSLLMIRQPIGEVTLTRMFAGQNLDRSR
                     AGIENLSIEKVILRNDGGAVLVTEVSYVTDYSYYDYFTQNYIRNFEYHFNNVITFSIN
                     ADGTIDWDAVLRKDQISTNDYGKYSSFALAMTENDISLLYNTRTDRNNGVAYFGIKRN
                     GETESKEIIKPEERILLVPFAAKQISTDEIVVPCWNRKKMLMAKITF"
     gene            1735..2514
                     /locus_tag="PK134_07355"
     CDS             1735..2514
                     /locus_tag="PK134_07355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB38701.1"
                     /translation="MPGSLSIALQYISYYVKAWNKHSVHSPFVYKLTTQVIHPDKKLA
                     KFIPIEQQRILLKQSTDKIFFTELGAGNKSNKRSVSEIAAKSLKFPRYARLLYRLANH
                     FQPDNIIELGTSLGITTGYMALSGCKNIFTIDGNKTVSEIAQSTLTSIKLHQNIKFLQ
                     GNFDEALPALLNNLSKVDFVFFDGNHRYQPTINYFHQCLTKAHDKSVFVFDDINYSQE
                     MQQAWHEIKQHPSVIVSIDLFMMGIVFFDTDLLRQHFVIRY"
BASE COUNT          917 a          435 c          434 g          821 t
ORIGIN      
        1 atataataaa ctgtgatgaa attacagagc ggttaattaa agaaacccat ctgtcggtcg
       61 aaaaccttag taataaattt aaggactaaa cctatccttc tttcttgggg aaaaggtgat
      121 tgaggtaaaa tgtcattttt aaagcctcat tacattcaat ttcatgcttc gttttcattt
      181 tagtttcagc aataatatat ctgtttttcg ttgaattagc ctacatttga acaaaaaata
      241 aatggccttt aaaagaatat tcttttttgt ttggttttca gtttgcttcc atgttgcaca
      301 tgcacaaaaa gttacttatg cacaacagga acttgatgcc tattcttatg attatataaa
      361 aacagcaggc tataacgata attccttttt tgtttttcaa agcaatctgc catttgaaaa
      421 cgatcgtgac agagtaggat tcaaatcacg taaatataaa gttacttgtt acaatctgaa
      481 tctatcttta aaatggtcga aaaaaattga acctaaaaat gataatgaga aattagaaac
      541 tataactgtg attgacggtc aggttgcttt aattaaatct tcttttgaag gtaaggacaa
      601 tcaattgtca gtgttcatta ccacattaga tgacaatgga aatgaatctg ctaccaagaa
      661 agaaattgga actattttat ttgacagaac aagcaactta gataagataa aagttattgc
      721 ttcgaaaggg aataactact ttggacttat tcaaaatgaa aagaaacaaa atggtttgca
      781 gcagctacat tgcctcataa ttgatagtga ctttaagctt gtccggcaac tgcaatttga
      841 agtaccatac cctgaaaaaa atttcaacta cgaaaactgg cttttgacag atgatgcaga
      901 ctttattctt gaaggaagtt actactcaaa agaaaaaagt gaaaaccgca aacgatggga
      961 tgcatacaag ttatttgtat cgtccaatgg aaatagtggc gccattgaat atgctatcaa
     1021 taccagcaac ctgatattaa gcagcagcac catagcttat gatgccataa ataagaaaat
     1081 agttcttgcg gctttttata ctgacaatgt ttctacaaaa tccggaataa tttatgcaag
     1141 tcttggaact tcaggaaatg attcgttgtt gatgataaga caacctattg gcgaagtcac
     1201 cttaacacgc atgtttgccg gtcaaaattt agaccgtagc agagcaggaa ttgaaaacct
     1261 gagcatagaa aaagtgattt tacgaaatga tggtggtgct gtattagtta cagaggtatc
     1321 ttatgttaca gactactcct actatgatta ttttacacaa aactacattc gtaatttcga
     1381 atatcatttc aataatgtca ttaccttttc tatcaatgcc gatggtacaa ttgactggga
     1441 tgcagtttta agaaaagatc agatttctac aaatgactat ggaaaatatt cttcgtttgc
     1501 cttagccatg acagaaaatg atatcagttt actctataac acccgcacag acagaaataa
     1561 tggtgttgcc tatttcggaa taaaaagaaa tggagaaaca gaatcgaaag aaatcatcaa
     1621 acctgaagaa cggattttac tggtgccatt tgctgcaaaa caaatcagca ctgacgaaat
     1681 tgttgtcccg tgctggaaca gaaaaaagat gctaatggca aaaattactt tctgatgccg
     1741 ggatcattat ctattgcatt gcagtatatt tcctattacg ttaaggcatg gaacaaacac
     1801 agtgttcatt ctccttttgt atataagctc acaacacagg ttattcatcc cgacaaaaaa
     1861 cttgcaaaat ttatacctat tgaacaacaa agaattcttt taaaacaaag tacagacaaa
     1921 atttttttta ccgaattagg tgctggaaat aaaagcaata aacgaagtgt ttctgaaatt
     1981 gccgcgaagt ctttaaaatt tccgcgatat gcgcgtttac tttataggct tgcaaatcat
     2041 tttcaacctg acaatattat tgagttaggc acatcattgg gcattacaac aggctatatg
     2101 gcattatcag gctgtaaaaa tatttttact attgatggta ataaaactgt atcagaaatt
     2161 gcgcaatcta cattaacctc gataaagctg catcaaaaca taaaatttct tcaaggaaat
     2221 tttgatgaag cacttcctgc attgttaaac aatttaagta aagttgattt cgtttttttt
     2281 gacggcaacc accgctatca accaacaatt aattattttc atcaatgctt aacaaaagca
     2341 catgataaaa gtgtgtttgt gtttgatgat attaactaca gtcaggaaat gcaacaagca
     2401 tggcatgaaa tcaaacagca tccatcagta attgtcagta ttgacttgtt tatgatggga
     2461 attgtttttt ttgatacaga ccttctgcgt caacattttg taatcaggta ttaacatttg
     2521 gtaacttaat cataagccac aatttattac ttttaccacg tacaaatcaa ttatgccaca
     2581 gtccattatt tcgctttcag atatttc
//
LOCUS       DAORQT010000229         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8100_length_2583_cov_5.355617, whole genome shotgun sequence.
ACCESSION   DAORQT010000229 DAORQT010000000
VERSION     DAORQT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8100_length_2583_cov_5.355617"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..2311
                     /locus_tag="PK134_07360"
     CDS             260..2311
                     /locus_tag="PK134_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38702.1"
                     /translation="MQINFKKLIPHGIAISIFLLLTAVYFLPLLEGKGLSQHDIAQWT
                     GMSKELTDFRAKYHVEPLWTNAMFSGMPAYQISTLYPANLIQYVNDLLFFFLPSPASY
                     VFLALLSFYILMLVLKFDYRMGIAGAIAFAFSSYNFVIIAAGHNSKAHAIALMPLVFA
                     GIILTMRGRFWFGGAMTAMALALQLYANHLQITYYLMIAILVMVIAYFIDAIMKKEIG
                     GFIKSASVLAIAAVIGILPNYTSLAATYEYGKYSTRGPSELTSKKESSGLDKDYAFSY
                     SYGISETFTLIIPRFHGGASVSPLDENSATYKALTSNGVPVQQAKSFIQAVPLYFGNM
                     LSTSGPPYAGAIICFLFVAGLMLIKGKEKWWLLVATILSFMLSWGENFLSFNELFFYH
                     FPGYNKFRAVSMILTIAQFTMPLLALLALKEIFDGKTNKNDILKSLKMALYITGGFCL
                     LFTLLPGMFNDFSSQADEQLKQYDWLITAIKQDRESAVRMDALRSLFFIAATFGVLWF
                     MLKDKIKATVAIITIAVLFTVDLWPVGKRYLNDNNFVAKSKTQKPFEPSPADTQILQD
                     TTPGFRVLNTTLNAFSDASTSYFHRSIGGYHGAKLKRYQELIENCISKNNMSVLDMLN
                     TKYLIVSDKEQQPVAQLNPGAIGAAWFTDTVKMVANADEEINALNSFNPRTIAFVDKR
                     F"
BASE COUNT          796 a          495 c          470 g          822 t
ORIGIN      
        1 ttacaggaca ggtgataaaa tctatctctg ttaatgcaaa cactaaaatt tctattgatg
       61 taaaagatct ttctgccggc atgtattttg ttacagctac aggcagtaat attgaaacac
      121 agcgcttcat aaaagcaaag taatttccct acaatagcta aaagccttag tatccccgat
      181 attaaggctt ttttaatgtc tgaaaaacaa cggaattatt cctttctaaa ctattacatt
      241 tgcccaataa agaaatcgta tgcagataaa cttcaagaag ttaatccctc acggcatagc
      301 catcagtatt tttttactct taacagccgt ttatttttta cctctattag aaggtaaggg
      361 actgagtcaa catgacattg ctcaatggac aggtatgagc aaagaactta ctgacttccg
      421 tgcaaaatat catgttgaac cattatggac aaatgctatg ttcagtggta tgcctgccta
      481 tcagatttcg actttgtatc cggctaatct gatacaatat gtaaatgact tattgttttt
      541 ctttcttcct tctcctgcat cctatgtgtt tcttgctctg ttaagttttt acattctgat
      601 gctggtgctg aaatttgatt acagaatggg tattgctggt gccatagctt ttgcattttc
      661 aagttataat tttgtaatca tagccgcagg gcataattca aaggcacatg ccattgcctt
      721 aatgccattg gtatttgcag gcatcatcct taccatgcga gggagatttt ggtttggtgg
      781 tgctatgaca gcaatggcac ttgcattaca actttatgcc aatcaccttc agataacata
      841 ctatctgatg attgcaattt tagtaatggt aattgcctat ttcattgatg ccatcatgaa
      901 aaaagagatt ggtggtttta tcaaaagcgc ctccgtactg gccattgccg cagtgatagg
      961 aattctgcca aactatacca gtcttgctgc aacttatgag tatggaaaat attcaacccg
     1021 tggccctagt gagcttactt caaaaaaaga atcatcaggc cttgataaag attatgcatt
     1081 tagctatagc tatggtatca gcgaaacatt tactctcatc attccacgtt ttcatggtgg
     1141 tgcatctgtc agtccgttag atgaaaattc tgctacatac aaggcattaa caagtaatgg
     1201 agtacccgtt caacaagcaa aaagttttat tcaggcagtg ccactctatt tcggaaacat
     1261 gctttcaacc tcgggcccac cttatgccgg tgcaataatt tgctttcttt ttgttgcagg
     1321 tttaatgctg ataaaaggaa aagaaaaatg gtggctgctg gtagcaacaa ttctttcatt
     1381 tatgctttca tggggagaaa attttctttc attcaacgag ttattctttt atcattttcc
     1441 gggatacaat aagttcagag ctgtgagtat gatacttaca attgcacagt ttaccatgcc
     1501 tttgcttgca ctccttgcat taaaagaaat atttgatggc aaaacaaaca agaacgatat
     1561 attaaagtca cttaaaatgg cattatatat taccggtgga ttttgtttgc tatttacatt
     1621 attaccagga atgtttaatg actttagctc gcaagctgat gaacaactga aacaatatga
     1681 ctggttaatt actgccataa aacaagaccg cgaaagtgca gtcagaatgg atgcattgcg
     1741 ctcattattt tttattgctg caacttttgg tgtattatgg tttatgttaa aagataaaat
     1801 aaaggcaacc gttgctataa taactattgc tgtccttttt actgttgact tatggcctgt
     1861 aggtaaacgt tatctcaatg acaacaattt tgttgcgaaa agcaaaacac aaaaaccatt
     1921 tgagccttca cctgctgata cacaaatttt acaggataca actcccggat tcagagtgct
     1981 taacacaaca ctaaatgcat tcagtgatgc atctacctcc tattttcatc gcagcatagg
     2041 tggctatcat ggcgcaaaat taaaacgcta tcaggaactg attgaaaact gtatctccaa
     2101 aaacaacatg agtgtgcttg acatgcttaa cacgaaatat ctgatagtaa gtgataaaga
     2161 acaacagcct gtagcacagc ttaaccctgg tgcaattggt gctgcctggt ttacagacac
     2221 tgtaaaaatg gttgctaatg ccgatgagga aataaatgca cttaactcct ttaatccacg
     2281 aacaattgca tttgttgaca agcgttttta aaatacatta aaatcctttt ctcctcaaca
     2341 cgactctact gctactgtta aacttaattc tattgtatgt aatcaccttg tatatgaaac
     2401 gcagacggca ttgccgcagt tctgtgtgtt ttcagaaatc tattatgaca aagggtggaa
     2461 cgcttacatt gacggacaaa aagctgacta cgtgcgagca aactacgtat tgcgtgccat
     2521 ggtagtccct gcaggaaaac ataaaataga gtttaagttt gaacccgaag tttattttac
     2581 agg
//
LOCUS       DAORQT010000230         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8167_length_2571_cov_5.144277, whole genome shotgun sequence.
ACCESSION   DAORQT010000230 DAORQT010000000
VERSION     DAORQT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_2571_cov_5.144277"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1353
                     /gene="dinB"
                     /locus_tag="PK134_07365"
     CDS             133..1353
                     /gene="dinB"
                     /locus_tag="PK134_07365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRB38703.1"
                     /translation="MSAQLNRVIAHMDLDCFFVSVSRLLHPELNNKPVIVGGGERGVV
                     AACSYETRKFGVHSAMPIKQAKRLCPEAIIVRGDYDAYSSHSDMVHEIICESVPLYER
                     SSIDEFYLDLTGMDRFFGCYKHATELRQRIIRETGLPISFGMSANKTVSKIATDEAKP
                     NNQMQVDCGEEKIFLAPLSVRKIPMVGEKTYTLLRSMGVEKVKTVQEMPVELMERVLG
                     ENGTTIWKKANGIDTTAVEPYHEQKSMSTEETFEQDTIDVQRLQHILIAMTEKLCFRL
                     RSEHKVTSCISVKIRYSNFDTHTMQCRIAYTSCDHIIIKQVKELFAKLFNRRMLIRLI
                     GIKFSHLVGGGHQINLFEDSEEIINLYQAMDRMRLRFGEDKIHRAAAMSFSLRGFNPF
                     NGLSNSPKEIKGGK"
     gene            complement(1510..2487)
                     /locus_tag="PK134_07370"
     CDS             complement(1510..2487)
                     /locus_tag="PK134_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB38704.1"
                     /translation="MLISVYVPYHAAIEAITPAYRLFKTANDILIASGKKPKFKVEYV
                     GLQKSIKANDGEYSIRIDRHINSVNDSDLIIIPALYGDVKHSLKKNAAAIPWLQKMHR
                     QGTEVASLCIGAFLLTASGLVDGKKVSTHWAYFNEFKEQFANTEVVDGAIITEERGIY
                     SSGGANSLWNLLFYLLEKYSDRHTAILASKYFAIDLDRNSQASFAIFNGQKNHHDTEI
                     LKAQQYIEKHTDAKLTIDQLADYTKMSRRTFERRFLKSTNHSVLEYIQRVKIETAKRK
                     FESSRKNITEVMFDVGYTDTKAFRDLFKKHTGITPLEYRNKYSKISLKG"
BASE COUNT          776 a          499 c          511 g          785 t
ORIGIN      
        1 gattgttaaa cgaacagatt gtaatttttt taagactaat aggtagtgac ttaaaaagaa
       61 agatatgttt tcagaaaaaa gtaatgacag aaaggtttat attgtagcca tcagaatgtt
      121 tgtattataa tcatgtcagc gcaacttaac cgtgtcatag cacacatgga cctcgattgt
      181 ttttttgtat ccgtatcgcg gctgctgcat ccggagctaa acaataagcc ggtaattgtt
      241 ggcggtggcg agcgtggtgt agtggctgca tgcagctacg aaaccagaaa atttggtgtg
      301 cactctgcca tgcccattaa gcaggcaaaa cgcctgtgcc cggaagctat tattgttcgt
      361 ggcgattatg atgcttacag cagtcattcc gatatggtgc atgaaattat ttgcgaaagt
      421 gtgccgctgt atgagcgtag cagtattgat gaattttatt tagacctaac aggcatggat
      481 cgtttttttg gatgttataa acatgctaca gaactcaggc aacgcatcat ccgcgaaaca
      541 gggctgccta tttcttttgg tatgagtgcc aacaaaaccg taagcaaaat tgctaccgat
      601 gaggcaaagc ccaacaatca gatgcaggtg gactgtggcg aagagaaaat ttttttagct
      661 ccgctgtcgg tacgaaaaat tcctatggta ggcgaaaaga cctacacact gctgcgcagc
      721 atgggtgtag agaaagtaaa aaccgttcag gaaatgcctg tagaactgat ggagcgtgtg
      781 cttggcgaaa acggaactac catctggaaa aaagccaacg gcattgacac cacagccgtt
      841 gagccttacc atgagcagaa gagcatgagc acagaagaaa cttttgagca ggacactatt
      901 gacgtgcaac gcctgcagca catcctcatt gctatgacag aaaaactatg cttccgcctg
      961 cgcagcgaac acaaggtaac gtcatgtata agcgtaaaaa tccgttacag caacttcgac
     1021 acacatacca tgcagtgccg catagcctac acaagttgcg accacattat tattaagcaa
     1081 gtaaaagaac tgtttgctaa gctgtttaac cgcagaatgc tcatcaggct tattggtatt
     1141 aagttcagcc atctggtagg tggcggacat cagattaatt tgtttgaaga cagcgaagaa
     1201 atcatcaacc tctatcaggc aatggaccgt atgcgcctgc gctttggcga agataaaatt
     1261 caccgtgctg ctgccatgag cttttcattg cgcggattca atccgtttaa tggattgagt
     1321 aattcaccta aagagataaa gggcggtaaa taagctaaaa aaatagttgt agaacgataa
     1381 taccttgaaa aggcaaaatt ccatatttac ttttacggtc ttaattttaa ttatatatat
     1441 tcataattaa acttaggtac ctactatgga gtttgataaa aacatttctg attaaacaac
     1501 acaaaacaat caacctttca aagatatctt actgtatttg ttcctatatt ccagcggagt
     1561 gatgcctgta tgttttttaa ataaatctct gaatgctttt gtgtcagtat aacccacatc
     1621 aaacattact tcagtaatat tttttctgct gctttcaaac ttgcgttttg cagtttcaat
     1681 ttttactcgt tgaatatatt ccaataccga atgattggtt gacttcagaa atcttcgctc
     1741 aaaagttctt cggctcatct ttgtatagtc tgcaagttgg tctatagtta gttttgcatc
     1801 agtgtgtttt tctatatact gttgtgcttt taaaatttct gtgtcatgat gatttttctg
     1861 accattgaat atggcaaagg aagcctgact gtttcgatcc aaatcaatag caaagtattt
     1921 tgaagcaaga atagcagtgt gtcggtctga atatttttcc aacaaataaa acagcagatt
     1981 ccacagcgag tttgcaccac cgctgctgta aatgccacgc tcttcggtaa tgattgcgcc
     2041 atcaactacc tctgtatttg caaactgttc tttaaactca ttaaaataag cccaatgtgt
     2101 tgacactttt ttgccgtcaa ccaaacctga tgctgttagt agaaaagcac caatacataa
     2161 acttgcaact tccgtaccct gacgatgcat tttttgtagc catggaatgg cagctgcatt
     2221 tttctttaag gaatgtttta catcaccata aagcgcgggt ataattatta agtcagaatc
     2281 gttaacacta tttatatgcc tatcaatacg aatgctgtat tcgccatcat tggctttgat
     2341 gcttttttgt aagcccacat attcaacttt aaacttaggt tttttacctg aggctataag
     2401 aatatcattg gcagttttaa ataatctgta tgccggtgtt atggcttcta tagctgcatg
     2461 atacggtaca taaacagaaa ttaacattcc ggtaattttt atttggtaaa gttaaagtat
     2521 tttgtcatta tcacacctta aaactgtcgt ttctgccacc tattgttgct t
//
LOCUS       DAORQT010000231         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8192_length_2565_cov_6.056574, whole genome shotgun sequence.
ACCESSION   DAORQT010000231 DAORQT010000000
VERSION     DAORQT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8192_length_2565_cov_6.056574"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..913
                     /locus_tag="PK134_07375"
     CDS             71..913
                     /locus_tag="PK134_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB38705.1"
                     /translation="MKHLIILLFSIVIVSCSKESEKETVYKNLEGNKTDTLQSIQSTV
                     KMVYIPGGEYKPFFGSNTTLVSVAPFLMDERAVTNEEFLEFVIANPQWQKSNIKRLFA
                     DTNYLKTWPTDITLPENALKDAPVCNVSWFAAKAFAQSVDKRLPTLDEWEFVAMADET
                     TANARKKPKYSDSIIDLYLQKNRQFKSVKQSPPNYWGIYNMFDLIWEWTDDFNAVLTT
                     GDSRSGEYNDKNLFCAGGATSATDVMNYAAYMRFALRTSIKADYTIGNLGFRCAKDTT
                     IIKQ"
     gene            926..1519
                     /locus_tag="PK134_07380"
     CDS             926..1519
                     /locus_tag="PK134_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRB38706.1"
                     /translation="MKLISLLCISLLMLVSCKENSEPKQGAETTEQLPSNSIFNLTSD
                     WQNQDGKTIKLKDLQGKTLVVVMIYTTCKTACPILVAKMKMIESKFDRKDIDKINLVL
                     VSIDPETDTPEHLKEFSIKNEMTGPQWTFLRSAENATKEFANVISMKYKKISPIDFSH
                     SNIISTFKPNGILVSQEEGDINVDKVVTTVNQTVRKK"
     gene            1625..2287
                     /locus_tag="PK134_07385"
     CDS             1625..2287
                     /locus_tag="PK134_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermine synthase"
                     /protein_id="HRB38707.1"
                     /translation="MAKRYSVFQWLFSFITPVIIHKDQSEVNSILETTLENGKLVLNS
                     AHANYSYGSLFRVFIKALLRFNIDIKNKKKILVLGMGGGSVVKFLLGNNTYAQLDTVE
                     IDRKIISIAKEMFDLSPSDRLKIYAANAVDYLKDCTEEYDLILIDLFIDDAVPEFCFT
                     ESFWKSIQNICLPKCDIVFNASMKQNNLQHNLDFLGAIHLKENLTIEQNLFFLLQPAV
                     SK"
     gene            2356..>2565
                     /locus_tag="PK134_07390"
     CDS             2356..>2565
                     /locus_tag="PK134_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38708.1"
                     /translation="MKRIYTTIFLTLVAISLQAQNATPNAGFENWNQVGNRFDPADWN
                     NLNPSTGIIGVLTCARASGADAHSGS"
BASE COUNT          913 a          435 c          433 g          784 t
ORIGIN      
        1 atgagaaagt attcagtgca aaaactctga caaaagagta acccgccttt tgtaagcaat
       61 aaaatgcaac gtgaaacact taatcatact tttattttct attgtaattg tatcttgctc
      121 taaggaatcc gaaaaggaaa ctgtttacaa aaacttagag ggcaataaaa ctgatacatt
      181 acaaagcata caaagtaccg ttaagatggt ttatattccc ggaggagaat ataaaccatt
      241 ctttggttct aacactacat tggtgagtgt agctcctttt ctaatggatg agcgagctgt
      301 gaccaacgaa gaatttcttg aatttgtcat tgcaaatcca caatggcaaa aatcaaatat
      361 taaaagactg tttgcagaca ccaactattt aaaaacatgg cctacagaca ttacattacc
      421 tgaaaatgca ttgaaagatg ctccggtatg taacgtatca tggtttgctg ccaaagcatt
      481 tgctcaaagt gttgataaac gtttgccaac attagatgaa tgggaatttg tagctatggc
      541 agatgaaaca acagccaatg caagaaaaaa accaaaatat tcagatagca tcattgattt
      601 ataccttcaa aaaaacagac aattcaaatc ggttaaacaa agtcctccaa actattgggg
      661 tatttataat atgtttgatc tgatttggga atggacagat gattttaatg ctgtactaac
      721 aacaggcgat tcgcgaagtg gagaatataa tgacaaaaat cttttctgtg caggtggcgc
      781 aacatctgca accgatgtta tgaattatgc agcatatatg agatttgcac tgcgtacatc
      841 catcaaagcc gattacacta ttggaaattt aggattccgt tgtgcaaaag acacaacaat
      901 tattaaacag taacactata attttatgaa actcatttca ctattatgca tttcactatt
      961 aatgcttgtt tcatgcaaag aaaattctga acctaagcaa ggtgcagaaa ctactgagca
     1021 gcttccatcc aactcaattt tcaatcttac ttccgactgg caaaatcaag atgggaaaac
     1081 tattaagcta aaagacttac agggtaagac actggtagta gttatgattt acactacctg
     1141 taaaactgca tgccctatac ttgttgctaa aatgaaaatg attgaatcta aatttgatcg
     1201 gaaagacatt gataaaatca atcttgtact agtatctatt gaccctgaaa cagacactcc
     1261 ggaacatctc aaagagtttt cgattaaaaa cgaaatgaca gggccacaat ggacattctt
     1321 acgctctgct gaaaacgcaa caaaagaatt tgcaaacgta atttcaatga aatacaaaaa
     1381 gatttcacct attgattttt cacactcaaa cattatcagc acctttaaac caaatgggat
     1441 tttagtaagt caggaagaag gtgatattaa tgttgacaag gttgttacaa cagtcaatca
     1501 aactgtaaga aagaaataaa acaattaaaa aagagaacaa tctcatttaa cataaaacta
     1561 ctttttaggc agtagttaac tccctttgct tccatagaat attctacgta gttatctttg
     1621 cataatggca aaacgatatt ctgtatttca atggttgttt agttttataa ctcctgttat
     1681 cattcacaaa gatcaatcgg aagtaaatag tattttagaa actacattgg aaaatggcaa
     1741 actggttctg aattctgctc atgccaatta cagctatgga agtctatttc gtgtttttat
     1801 aaaagcattg cttcgtttta atattgacat caaaaacaaa aagaaaatat tagttcttgg
     1861 tatgggcggt ggcagtgttg taaagttttt attaggaaac aatacatatg cacaacttga
     1921 tactgttgaa attgaccgga agataattag cattgcaaaa gaaatgtttg acctttctcc
     1981 ttctgataga ttaaaaattt atgcagctaa tgccgttgac taccttaaag attgtacaga
     2041 ggaatatgat ttaattttga tagacctttt tattgacgat gctgttcctg agttttgttt
     2101 cacagaatct ttttggaaat caattcaaaa catttgtctg cctaaatgtg acattgtttt
     2161 taatgccagt atgaagcaaa ataaccttca acacaatctt gattttttag gcgcaataca
     2221 cttaaaagag aatcttacca tagaacaaaa tctgtttttc ctgttacaac ctgctgtgag
     2281 taagtaattt actatcacta taaaatttta attcattgca ctatatttgt ccggttaatg
     2341 aaaactcaat tactaatgaa aagaatttac acaactatat ttttaacttt agtagccatt
     2401 agccttcagg cacaaaatgc aacgcctaat gcaggttttg aaaactggaa tcaggttgga
     2461 aatcgttttg accctgccga ttggaataat ctgaatccaa gcaccggaat aataggtgtg
     2521 ttaacttgtg ctcgtgcttc aggtgcagat gcacacagtg gaagc
//
LOCUS       DAORQT010000232         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8207_length_2563_cov_5.399920, whole genome shotgun sequence.
ACCESSION   DAORQT010000232 DAORQT010000000
VERSION     DAORQT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8207_length_2563_cov_5.399920"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PK134_07395"
     CDS             complement(<1..576)
                     /locus_tag="PK134_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRB38709.1"
                     /translation="MRAKVSLIAIAFVAAFQFAKAQDNKFNFGLKISPTVAWFKASDN
                     LDNDGSKIGFAYGLIADINFTNNYAFETGIDVTYRGGKLKYMDIYKMKTVLQYIELPL
                     TLKLKTNEIGYMTYFGKVGFTTGINIRTDGDFKKSDINPLNMALVAGIGTQYSLGGKT
                     ALLFGITFNNGFLDVVKEKHSKATSNFLAVDL"
     gene            610..>1280
                     /locus_tag="PK134_07400"
     CDS             610..>1280
                     /locus_tag="PK134_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38710.1"
                     /translation="MKFSTVRQTVYLLLSTVLMLGFSCQNDPHGDKHTKDTMTTHLKF
                     DEVLFSADSINPQHVVQWKQQYPYFFNLFVNRIIHLPPTDSAALALNLQKFVGDKDVK
                     DIHARVEKVFSAADKDKLFSEIEGFLNNYKKEFGIQPVKHLVTFLSAFNYAVITADST
                     IGIGLDMYLGSDCEFYPSIGIPQYAYQRFSKDYILSDVIKGWYQSEHDVDAEKNDFLS
                     RMIYYG"
     assembly_gap    1281..1290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1291..1632
                     /locus_tag="PK134_07405"
     CDS             <1291..1632
                     /locus_tag="PK134_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldB"
                     /protein_id="HRB38711.1"
                     /translation="LYFTSVMAPQVQDTILTGYSKKQLEWCSNNSKQMWAFFIENKLF
                     YSTREMEYMKYIADGHATQGFPEGAPARTAQWLGWKIVSTYMKNNNVTLKELLAEKDA
                     QRILEKSGYKP"
     gene            1663..1992
                     /gene="gldC"
                     /locus_tag="PK134_07410"
     CDS             1663..1992
                     /gene="gldC"
                     /locus_tag="PK134_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRB38712.1"
                     /translation="MKKSEIKFTVSLDDNNKPVALHWNAEDSGMQGDKECKAMMLALF
                     DKKDQTTMRIDLWTQEMMVEEMQQFFFEMYMSMADTFQNATNNKDMADKMRNFARQFG
                     KEVSLIK"
     gene            complement(2018..2425)
                     /locus_tag="PK134_07415"
     CDS             complement(2018..2425)
                     /locus_tag="PK134_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB38713.1"
                     /translation="MTTHILYTEFILYVNNLQASTVFYSKLFRKIPDLNASGMTEFIL
                     SPNCKLGLMPDNGIAKIISEKMPHPNKGTGIPRCELYFYVDDIEAEYANALKCGAKLI
                     SPIADRDWGDKVCYFADNDGHIIAFAVKQNSAK"
BASE COUNT          857 a          437 c          464 g          795 t
ORIGIN      
        1 taaatcaaca gctaaaaagt ttgacgttgc ttttgaatgt ttttctttaa ctacatcaag
       61 aaaaccatta ttaaaagtta taccaaaaag taaggctgtt tttccaccta atgaatattg
      121 tgttccaata ccggcaacta atgccatgtt caatgggttg atatccgatt ttttaaaatc
      181 accatctgtt ctgatattga tacccgttgt aaatcccacc ttaccaaagt aagtcatata
      241 accaatttca ttggttttca gctttaaggt taaaggaagt tcaatgtatt gcaatacggt
      301 tttcatttta tagatgtcca tatatttcaa tttgccacca cggtaggtaa catcaatgcc
      361 ggtttcaaat gcatagttgt tggtaaaatt aatatcggca atcagtccat aagcaaaccc
      421 tattttgcta ccatcattat ctaaattatc gctggcttta aaccaggcta ccgttggtga
      481 aattttaagt ccgaaattaa atttgttgtc ctgcgctttt gcaaattgaa aagcagctac
      541 aaaggctatt gcaattagtg atacttttgc tctcattatt ttatgttttt ttaattcaag
      601 gcaaaattaa tgaaattttc cacagtcaga caaacagttt atcttttact atcaacagtg
      661 cttatgctgg gtttttcttg tcagaacgac ccgcatggag ataaacatac taaagacaca
      721 atgaccacac atttaaaatt tgatgaagtg ttgttttctg ctgacagtat taacccacag
      781 catgtggtgc aatggaaaca acaatatcct tattttttca atctgtttgt aaacagaata
      841 atacatctgc cgccaacaga cagtgctgca cttgcattaa atcttcaaaa atttgttggt
      901 gataaagatg taaaggacat tcatgctcgg gttgagaaag ttttttctgc tgccgataaa
      961 gataaattgt tctctgaaat agaaggattt ttaaacaatt acaaaaaaga gttcggcata
     1021 caaccggtaa aacatctggt aacttttctt tctgcattta actatgctgt tataactgca
     1081 gacagtacca ttggtattgg gctcgacatg tacttaggca gcgattgtga gttttatcca
     1141 tcaataggaa tacctcaata tgcttatcaa aggttttcaa aagattatat tttgagtgat
     1201 gtgataaaag gctggtatca gagtgaacat gatgtggatg cagaaaaaaa tgatttcctc
     1261 agccgcatga tttattatgg nnnnnnnnnn ttgtatttta cttcagttat ggcaccacaa
     1321 gtgcaagaca ctattctgac tggatacagc aaaaagcaat tggagtggtg cagcaacaac
     1381 agcaaacaga tgtgggcatt ttttatagag aacaaacttt tttattctac ccgcgaaatg
     1441 gaatacatga aatacattgc agacggacat gccacacaag gttttcctga aggggcacct
     1501 gcacgcacag cacagtggct gggatggaag attgtaagca cctatatgaa gaacaacaac
     1561 gttacattaa aagagttgct tgcagaaaaa gatgcacagc gaatactcga aaaatcagga
     1621 tataaacctt agttcaaatt taaaataaca acaatcagaa agatgaaaaa atcagagata
     1681 aaatttactg taagcttaga cgacaacaac aaacctgtgg cattgcattg gaatgccgaa
     1741 gacagcggca tgcaaggcga caaagaatgt aaagccatga tgcttgcttt gtttgataag
     1801 aaagatcaaa ctaccatgcg cattgactta tggactcagg aaatgatggt agaagaaatg
     1861 caacagttct tttttgaaat gtatatgagc atggccgata ctttccagaa tgcaactaac
     1921 aataaagata tggccgataa aatgcgaaac tttgcaagac agtttggaaa agaagtgagt
     1981 ttgattaagt aactatttct taatttctta ttctttttta tttggcagaa ttctgtttca
     2041 ctgcaaaggc aataatgtgt ccatcattat ctgcaaaata gcaaacttta tcgccccaat
     2101 ctctgtctgc aatagggcta ataagttttg ctccacattt tagggcattt gcatattcag
     2161 cctcaatatc gtcaacataa aagtataatt cacatcttgg aattccggta cctttgttgg
     2221 gatgtggcat tttttcagaa atgatttttg caataccatt atcaggcatc aaccctaatt
     2281 tgcaattagg agaaagtata aactctgtca ttcctgatgc attcaaatcc ggaatttttc
     2341 taaagagttt tgaatagaat acagtgcttg cctgcagatt gtttacataa agaataaatt
     2401 ctgtgtatag aatatgtgtt gtcattatag gatattctga atccgtttca tagtcactgc
     2461 gaaggggcgg gagattatga aactacaaag gtacttagaa acttatgata ttgacaacat
     2521 caaagatgcg aaacaatttt taaagactgt agtagatcta tac
//
LOCUS       DAORQT010000233         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8308_length_2544_cov_5.985536, whole genome shotgun sequence.
ACCESSION   DAORQT010000233 DAORQT010000000
VERSION     DAORQT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8308_length_2544_cov_5.985536"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1391)
                     /locus_tag="PK134_07420"
     CDS             complement(123..1391)
                     /locus_tag="PK134_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38714.1"
                     /translation="MKVFSYLILSFIFSIHSVECKTVRALFIGNSYTDVNNLPEIVKQ
                     IALSTGDTLIYSKNAPGGYTFLMHTTNATTISLIQAGNWDYVILQEQSQLPSFPDNDV
                     ATQVYPYAAALDSMIKASSPCATTLFYMTWGRQNGDAANCPFFPPLCTYLGMDSLLQL
                     RYTIMAQDNHAAISPVAKVWRKIRSDFPTLNLYASDGSHPNNNGSFAAACSFYSVMFG
                     KNPQLASYNFTVTASDAVTIKTVAKEVAFDSLNYWFQYDALIADFNFSTVTNTVSFFN
                     TSQNATTFNWTFGDGSSSTLMNPSHTYTASGTFNITLIASDSVCGFADTVSKTITIST
                     SGLAAPESSNSINILPNPVLDVMTFTSVKTVSSFQIFTTDGRLILTSNRPIQSNEEHS
                     LNVSMLSSGIYFLKVYSDELNASCIKFTKF"
     gene            complement(1406..1816)
                     /gene="rnk"
                     /locus_tag="PK134_07425"
     CDS             complement(1406..1816)
                     /gene="rnk"
                     /locus_tag="PK134_07425"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRB38715.1"
                     /translation="MSKLIINRLDYARIKKSISDAKQFKSISNSEAEKLQQELDAAKI
                     VEPEAIPSNVVTMNSIVKLSFLNNDKKIQFQIVYPEQANIKENKISIFSPIATALIGY
                     KVNDEVEWIVPAGLTRIRIDEIVYQPEAAGDFDL"
     gene            complement(1938..>2544)
                     /locus_tag="PK134_07430"
     CDS             complement(1938..>2544)
                     /locus_tag="PK134_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRB38716.1"
                     /translation="KSDFDLNFQNKSVDGRIVAALERISQAFRVLLWQESKDFSLSPI
                     QIQVIIFLLHHSDDKCKISYLADEFNMTKATISETVKTLEHKKLIQKVYEEFDNRSYT
                     IQLTKSGNAIATQTALFSKEFHKPIAKLSNDGKENMLLSLLNIINHLNKTGVITIQRM
                     CLSCTHHALTTNGENHFCKLLNRELLSSELRIDCPEHVLST"
BASE COUNT          847 a          425 c          429 g          843 t
ORIGIN      
        1 ggtatgaaaa agtaaaatca gattttatcc ttattagaaa ttcagatttt acaaattaaa
       61 tccaatacca gaaaattcat tattaaaata acttgcaaat tcctctatag taattttctt
      121 gtctaaaatt ttgtaaactt gatacatgat gcatttaatt catcactgta aaccttcaaa
      181 aaataaatac ctgacgatag catagaaaca tttagtgaat gctcctcatt agattgaatt
      241 ggtctattag aagttaatat aagccttcca tcagttgtaa aaatttggaa tgatgaaact
      301 gttttaactg atgtaaaagt catgacatcc aaaacagggt taggcaaaat attaatgcta
      361 tttgaagatt caggtgctgc taaaccggaa gttgaaattg taattgtttt tgaaacagtg
      421 tctgcaaaac cacaaacaga atcagaagca atcaatgtaa tattaaaagt gccacttgca
      481 gtgtaagtat gtgatgggtt catcaatgtg ctgctactgc catcaccaaa tgtccaatta
      541 aaggtagtag cattttgcga agtattgaaa aaactaactg tattggtaac agttgagaaa
      601 ttaaagtctg caatcaatgc atcgtattga aaccaataat ttaagctatc aaatgctact
      661 tcttttgcca ctgttttaat tgtgaccgca tctgatgctg taactgtaaa attatatgac
      721 gctaattgag gattctttcc gaacataact gagtagaaac tgcaagctgc agcaaaagat
      781 ccattattgt ttggatgact gccatcactt gcatataggt ttagcgttgg aaaatctgat
      841 cgtattttgc gccatacttt tgctaccggt gaaatggctg catggttatc ttgcgccatg
      901 atggtatatc tcaactgtaa caaactatcc atacccaaat aagtacatag tggtggaaag
      961 aacgggcaat tggcagcgtc accattttgt cttccccagg tcatataaaa aagtgtagtt
     1021 gcacaagggc tacttgcttt tatcatactg tctaatgctg ctgcataggg ataaacctgc
     1081 gttgctacat cattatcagg aaatgagggc agctgacttt gttcctgaag aataacatag
     1141 tcccaattac ctgcctgaat taagctaatt gtggttgcat tggtagtatg cattagaaaa
     1201 gtatatcctc cgggtgcatt cttactatat atcagtgtgt caccggtaga aagtgctatc
     1261 tgttttacaa tttcaggtaa gttatttaca tcagtataac tgttgccaat aaataatgct
     1321 cttacagttt tacattcaac agaatgtata gaaaatataa aagataaaat taaatatgaa
     1381 aatactttca tggattcagg taattttaaa gatcaaaatc accggcagct tccggctgat
     1441 acacgatttc atctatgcgt attcttgtta atccggcagg aactatccat tcaacttcat
     1501 cattaacttt ataaccaatg agtgcagttg ctatgggaga aaaaatagaa attttattct
     1561 ccttaatatt agcttgctcg ggatatacta tctgaaactg aattttttta tcattgttta
     1621 aaaaactaag ttttacaatt gagttcatgg taaccacatt agaaggtatt gcttcaggct
     1681 caacaatttt agcagcatct aattcttgct gtaacttttc tgcttcggaa ttgctgattg
     1741 acttaaattg tttggcatcg ctgatagact ttttaatacg cgcatagtcc aaacgattga
     1801 taattaactt actcataaca aataattata ataaaattag cctgcaaaga taaggtattc
     1861 caagctattg aaatatttaa aaagcagatg tgtatgaaaa aacaatgctc ttaaaagatt
     1921 ttacatggtt aatacaacta agtgctcaaa acatgctccg ggcagtcaat acgcagttct
     1981 gaggaaagca gttcacgatt tagtaattta cagaaatgat tttctccatt tgttgttaat
     2041 gcatgatgtg tgcaggacaa acacattcgt tgtatagtga tgacacccgt tttgttgaga
     2101 tgattgataa tatttagaag actcaacaac atgttttctt taccatcatt actgagtttt
     2161 gcaattggct tatgaaattc tttactaaat aaagctgttt gtgtggcaat ggcatttcct
     2221 gactttgtta actgaatagt ataacttctg ttatcaaact cttcataaac tttctgaata
     2281 agtttcttat gttccaatgt ttttacagtc tcactgattg tagctttggt catattaaac
     2341 tcatcagcca gataacttat tttgcattta tcatcactgt gatgaagcag aaaaataata
     2401 acctgaatct gaattggact taaagaaaaa tctttactct cttgccatag taatactcga
     2461 aatgcctgtg aaattctttc aagggctgca acaatacgcc catcaacgct tttattttga
     2521 aaattcagat caaagtcaga cttg
//
LOCUS       DAORQT010000234         2533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8349_length_2533_cov_6.387006, whole genome shotgun sequence.
ACCESSION   DAORQT010000234 DAORQT010000000
VERSION     DAORQT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8349_length_2533_cov_6.387006"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1069
                     /locus_tag="PK134_07435"
     CDS             197..1069
                     /locus_tag="PK134_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRB38717.1"
                     /translation="MSHQKAIQTTWISIAGNILLAVVKWITGYFGNSYALIADAIEST
                     TDIFSSLLVLLGLKYASRPADKNHPYGHGRVEPLVTFLVVGILVASATLIAVESIRNI
                     MTPHQTPESYTLIVLAVIIIFKELFFRFVKKRNSQINSTALKADAWHHRSDAITSLAA
                     FIGIAIAIFMGKGFEAADDWAALVAAGFIFYNSYLIFRPALGEIMDEHLYEDVVEDIR
                     KVAGKVEGVIDTEKCYVRKTGINFLVDLHITVSSHISVKAGHQIAHHLKDVLIKHNPL
                     IADVLIHVEPDEHV"
     gene            complement(1095..1961)
                     /locus_tag="PK134_07440"
     CDS             complement(1095..1961)
                     /locus_tag="PK134_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRB38718.1"
                     /translation="MRQLKITKSITNRESASFEKYLQEIGKEELITAQMEVELARKIR
                     AGDQRALEKLVRANLRFVVSVAKQYQSQGLSLPDLINEGNLGLIKAAKRFDETRGFKF
                     ISYAVWWIRQSIMQALAEQARIVRLPLNQIGAANKVKKAFSSLEQKFEREPTAEELAN
                     ELEIDTDRIAYTMKMPGRHVSMDAPFVPGEDNTLLDVLTNPDAPYADSTLMAESLHTE
                     INRSLETLPEREKDVIKLFYGIGVEHGLSLEEIGEKFDLTRERVRQLKEKAIKTLRQN
                     SKSKLLKAYLGQ"
BASE COUNT          775 a          447 c          425 g          886 t
ORIGIN      
        1 taattataca ggtcattatt agaataaaag ttttgactaa agtaactaat attttataac
       61 tatgtattaa ttgctgccat ttcttgattg atgcttttgc ctataaagta tccatctttc
      121 aaaatggata aatctttaat tcagacgaag aaaatatttt ataaaactat aactttgaag
      181 tttcaattac aaaaacatgt cgcatcagaa agcgattcaa acaacttgga ttagcattgc
      241 aggcaatatt ttgctagcag tagtgaaatg gattacagga tattttggta attcgtatgc
      301 actgattgca gatgctattg aatctacaac agatattttt tcatcgttat tggttttatt
      361 ggggttgaaa tatgcaagtc gtcctgctga taaaaaccat ccttatggtc atggacgtgt
      421 tgaaccttta gtaacatttc ttgtggtagg aattttagtt gcttcagcta cactaatagc
      481 tgttgaaagc atcagaaata ttatgacacc gcatcagacg cctgaatctt atacacttat
      541 tgtccttgct gtgattatta tttttaaaga attatttttt cgcttcgtaa aaaagagaaa
      601 tagtcaaatt aatagtactg ccttaaaagc agatgcctgg catcatcgaa gcgatgctat
      661 tacttcgctt gcagctttta ttggaattgc aattgccatt ttcatgggta aaggatttga
      721 ggctgctgat gattgggctg cattggttgc tgccggtttt attttttaca acagctactt
      781 gattttcaga cctgccttag gcgaaattat ggacgaacac ctttacgaag atgttgttga
      841 agacataaga aaagtggctg gaaaagttga aggtgtaatt gatacagaaa aatgttatgt
      901 acgtaaaaca ggtataaatt ttttagttga ccttcacatc acggttagct cacatattag
      961 tgttaaagcc ggacatcaga ttgcacatca cttgaaagat gtgctgatta aacacaaccc
     1021 tcttatagca gacgtgctca tccatgttga accggatgag cacgtttgaa ttttaaaaca
     1081 ggaatgtttt ctgcttactg acctaaataa gctttaagca gtttgctttt tgaattttgg
     1141 cgaagtgtct taatagcttt ttctttaagc tgacgtacac gctcgcgtgt taaatcaaac
     1201 ttctctccta tttcttctaa tgataaacca tgctcgacac caataccgta aaaaagttta
     1261 ataacatctt tttcacgctc aggcagtgtt tctaatgaac ggttgatttc agtgtgtaac
     1321 gactctgcca tgagtgtgct gtcagcataa ggtgcatcag gattggttaa tacatcaagc
     1381 aatgtattat cttctccggg tacaaaaggt gcatccattg atacgtgacg tcccggcatt
     1441 ttcatagtgt atgcaattct gtctgtatca atttctaatt cattagctaa ttcttctgct
     1501 gttggttcgc gctcaaattt ttgttcaaga ctcgaaaatg ctttttttac cttgttggca
     1561 gcaccaatct gattaagtgg taacctaact atacgtgctt gctctgccaa tgcctgcatg
     1621 attgactgac gaatccacca tactgcatag gaaataaatt taaaacctct tgtttcatca
     1681 aaacgttttg ccgctttaat taaaccaaga tttccttcat taatcaagtc gggcaaactt
     1741 aaaccttgcg actgatactg cttagctacc gaaaccacaa atctcaggtt agctctcact
     1801 aatttctcta atgctcgctg gtctcctgct ctgattttac gggcaagttc cacctccatc
     1861 tgtgctgtaa ttaactcctc tttaccaatt tcctgtaaat acttttcaaa cgatgcgctt
     1921 tcacggtttg taatggattt agtaattttt agctgtctca tctattttat ctaattttta
     1981 tgtgtgtttc aatgtatgca atgtaagaac aaaaaacgat aatcatggtt taaaattgca
     2041 ttattttttt taaaggcgtt gcaaatatac tatgcgttta gcggcaatgc taactttatt
     2101 tttaagcaat gccacacaat gctgcttatc aacacattat ctttgccttt tcaagtaaaa
     2161 gactattttt ttaaccgatt ttgagagtta tttagcggtt atttatgatt aaaaattcac
     2221 ctgaaaacag aaaaattatt tcaatggtta ttcatcttta atccaaattt catcctttga
     2281 tgcaattaag agtaactgta agaaacagcg tataaagtat atttttcagt agttatatgg
     2341 ccacctttaa accctaaaaa gataaattag tacctgctaa aaaatatttc ttctacattc
     2401 gtgttttatt cgaacagaaa ttaaaacaaa ttggcattta acaacaacca catcatgata
     2461 aaaacgacca atatttttac tgcattagta tgtttattgt tagttacttc attgtcagta
     2521 ttaggacaaa aag
//
LOCUS       DAORQT010000235         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8393_length_2523_cov_4.612642, whole genome shotgun sequence.
ACCESSION   DAORQT010000235 DAORQT010000000
VERSION     DAORQT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8393_length_2523_cov_4.612642"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK134_07445"
     CDS             <1..423
                     /locus_tag="PK134_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRB38719.1"
                     /translation="VMHSIWSPEVFAVFLCRVVLGILFLFQGYEKLFTIGITKVTEIY
                     RSELVKKGFPLSVVKPLLTLSSFIEFAGGFLLIIGLFKYYSLYLLGLNMIGASLAFSI
                     MRPMWDLQHVFPRLALLVTLLILPAAWDLISIDHFLIK"
     gene            440..>792
                     /locus_tag="PK134_07450"
     CDS             440..>792
                     /locus_tag="PK134_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB38720.1"
                     /translation="MKIILCPTDFSSNATHAIAYASEMALKTNASLILLHTFETPILY
                     SDVAFVTVQYDYKVLHDAAAKKLKKFKEKFFVNKYAKLKVELILQQGLASARAMETAS
                     EKKVDMIVMGTTGTGA"
     assembly_gap    793..822
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <823..1274
                     /locus_tag="PK134_07455"
     CDS             <823..1274
                     /locus_tag="PK134_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB38721.1"
                     /translation="KNAPCPVLLIPPRVKFNGLKKLVYATDLSDDNLSKASELIPFAK
                     AFNSELLFLYIDTTTLSKDEIDVKKITDLVRKKVKYPKQSGYVCTDVSIQNGVSHFLK
                     KFKADALVMYTRHRNLFKGLYKPSIAKSVSYNTKVPLLVVHENDTLF"
     gene            1425..>2523
                     /locus_tag="PK134_07460"
     CDS             1425..>2523
                     /locus_tag="PK134_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HRB38722.1"
                     /translation="MQNLIKSKLAMLIIAVFLYGQSIAGNNCWKPVEEVSLSGMGKMN
                     RNTIPSTARYYELNMQQLQSLLQQAPNRGQQSNLIIDFPDAYGNMNAFRVYASPIMPE
                     MLSSKYPMIKTYCAQGINDPTATMRFSVTQFGLHCMTLSGTNPTNYIDTYTKDLNYYI
                     VYDKASLANNVRNFECLTDEDAELPSMRNGVAGINNNTDDQLLRTYRLALTCTAEYGN
                     IFATTPGSEKADIMAQMTNTMNRVNGVYERDLAITMEFVPNNDTLIFYGNTSQDPWSN
                     EWNTKTAQVCDQYIGVANYDIGHNFNTTGGGNAGCIACVCLSSSQSGTHKGRGMTGSA
                     NPVGDPFDIDYVAHEMGHQYGGYHIMNTCSRS"
BASE COUNT          809 a          464 c          467 g          753 t
ORIGIN      
        1 gtcatgcaca gtatttggtc accagaagtt tttgctgtat ttctatgcag agtggttctt
       61 ggcatcttgt ttttgtttca gggttatgaa aaacttttta caataggcat aactaaagtg
      121 acagaaattt accgttcgga attagtgaaa aaagggttcc ccctttccgt tgtaaaacca
      181 ctgctgacat tatcatcatt tatagaattt gctggaggct ttttattaat tatcggtctc
      241 tttaagtatt attctttata tcttcttgga ttgaatatga ttggcgcatc tcttgcattc
      301 agcattatgc gacctatgtg ggatttgcaa catgtttttc ccagattggc gttgttagtt
      361 acactactca tattacctgc tgcatgggat ttaatatcta tagatcattt cttaataaaa
      421 taacaaaacc attattgata tgaaaattat tctttgtccg acagacttct cttcaaatgc
      481 aacacatgct attgcatatg ccagcgaaat ggcattaaaa acaaatgcaa gtttaatact
      541 tctgcatact ttcgaaacac caattttata cagtgatgta gcgtttgtaa ccgtacaata
      601 tgattacaaa gtgctgcatg atgctgcagc aaaaaaacta aaaaagttca aagagaaatt
      661 ttttgtaaat aagtacgcta aactcaaagt agagttgata ctgcaacaag gattagcatc
      721 tgcacgcgcg atggaaactg cctctgaaaa gaaagttgac atgattgtta tggggactac
      781 aggcaccggt gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaaaaat gcaccatgtc
      841 ctgtacttct tattccaccg cgtgtaaaat tcaatggact aaaaaaactt gtttatgcaa
      901 ctgacctttc tgatgacaac ctgagcaaag catcagaact gattccattt gctaaggcgt
      961 ttaacagtga acttttattt ctttacattg atacaacaac tttatctaaa gatgaaatag
     1021 atgttaaaaa aataacagat ctggttcgca aaaaggtaaa atatccaaaa caatcaggat
     1081 atgtatgtac tgatgtttct attcagaatg gtgtgagtca tttccttaag aaattcaaag
     1141 ctgacgcctt ggtgatgtac acccgtcatc gcaacttatt taaagggtta tataagccaa
     1201 gtatagcaaa aagcgtgtcc tacaacacca aggttccttt gctggtagtg catgaaaacg
     1261 acaccctttt ttagccttat aaggtactga gattactcat ttattaactg attgcaaact
     1321 gtaattgtac ttgtttctgt tactgctata ttagcggaat aatcggtgca tgaggtcatt
     1381 tcatacacca tttccaaatc aaacaaaaac aaatcaaatt acacatgcaa aacttaatta
     1441 aaagcaaatt agccatgcta atcattgcag tatttctcta tgggcaatcc attgcaggga
     1501 ataactgctg gaaaccggtt gaagaggttt ctttatcagg aatgggaaaa atgaatcgaa
     1561 acaccattcc gtcaactgcc cgttattatg aattgaacat gcagcagttg caaagtttat
     1621 tacagcaggc accaaatcgt ggtcagcagt caaatctgat tatagatttc cctgatgctt
     1681 acggaaacat gaatgccttc agagtttatg catcaccaat aatgcctgaa atgctttcat
     1741 ccaagtatcc gatgattaaa acatattgcg cacaaggtat aaatgaccca acagcaacaa
     1801 tgcgttttag tgttacacag tttggtctgc attgtatgac tttatcagga acaaatccga
     1861 caaattatat tgacacttat acaaaggatt tgaattatta cattgtttat gacaaagctt
     1921 ctttagccaa taacgtccgc aattttgaat gtctcactga tgaagatgca gaactaccaa
     1981 gtatgcgaaa tggtgttgca ggtataaata acaacactga tgatcagtta ttgagaacct
     2041 acagacttgc acttacctgc actgccgaat atggaaatat ttttgccaca acaccgggaa
     2101 gtgaaaaagc cgatatcatg gcgcaaatga caaataccat gaatcgtgta aatggtgttt
     2161 acgagcgcga ccttgcaatt actatggaat ttgtgcccaa taacgacaca ttgatttttt
     2221 atggaaacac ctctcaggat ccatggtcaa atgaatggaa tacaaagaca gcacaggttt
     2281 gcgatcagta cattggtgtt gccaactacg acattggaca taactttaac accacaggcg
     2341 gtggtaatgc aggttgtatt gcctgtgttt gtttaagctc ttcgcagagt ggaacacata
     2401 aaggtcgtgg catgacagga agtgcaaatc ctgttggcga tccattcgat attgactatg
     2461 ttgcacatga aatgggacat cagtatggtg gctatcacat catgaacaca tgcagccgca
     2521 gtg
//
LOCUS       DAORQT010000236         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8471_length_2507_cov_3.481240, whole genome shotgun sequence.
ACCESSION   DAORQT010000236 DAORQT010000000
VERSION     DAORQT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_2507_cov_3.481240"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PK134_07465"
     CDS             complement(<1..992)
                     /locus_tag="PK134_07465"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRB38723.1"
                     /translation="MIEVKEMKETIATANSVFAQVTEMKHEQVVFCYDHETGLKAIIA
                     IHNTNLGPALGGTRMWMYQNENDALTDVLRLSRGMTYKAAISGLNLGGGKAVIIGDSK
                     KDKTEALFRKFGQYVNSLSGRYITAEDVGTSTKDMEYISKETKHVTGLPESMGGSGDP
                     SPVTAYGVYMGMKASAKEVWGNDSLSGKKVCVQGAGHVGSNLVKHLTEEGAKVYVTDI
                     NKDALQQVATTYKAEVVAPDSIFSLDVDIYAPCALGATLNTENISKLKCAIVSGAANN
                     QLADEVVHSKMLMDKGILWAPDFLINAGGLINVYSELKKFSRAEALKQTEHIYNV"
     gene            1102..>2507
                     /locus_tag="PK134_07470"
     CDS             1102..>2507
                     /locus_tag="PK134_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRB38724.1"
                     /translation="MSDLLTLNRFFVKYRGRLALGLIFVLLANYFGLLPAQITRKAID
                     FISTGTHHNYSDSAKTLTFYALGIFGVVFLRGVLLFLMRQTIIVMSRHIEFDMKNEIY
                     DQYQKLDMHFYAHANTGDLMNRISEDVSRVRMYTGPAIMYLVNITVMIIVVFIAMMNI
                     SPGLTLVVLFPLPLLVILIYYVQKIINTKSEKVQEALSTLSTNVQESFAGIRIVKSFS
                     KENDFAALFHNQAKNYFTQTMQLARINALFIPAIMLLVGISSVTVIYYGGILVDEKQI
                     TYGNIAEFVIYLNMLMWPVGMLGWIITLIQRADASQRRINSFLHTEPEIQCNNTGIVP
                     DKIKSIELNNISFSFGKNNDSALENINLQLSTGKVIAVVGTTGSGKTTIANLMSRLYD
                     PTQGEILVNDIPLKNINLEWWRRQVGFITQDVLLFSDTIANNIAFGIKEYNSETLTEA
                     AKTAMVYEEIVHFKDGFNT"
BASE COUNT          740 a          512 c          434 g          821 t
ORIGIN      
        1 acattgtata tatgttctgt ttgttttaac gcttctgcac ggctgaattt cttcaactcg
       61 ctgtaaacat taatcagtcc acctgcatta attaaaaagt caggcgccca taatatgcct
      121 ttgtccataa gcattttgct atgtacaact tcatctgcca actgattatt tgctgcaccg
      181 gaaacgatag cacacttaag cttactaata ttttcagtgt tcagcgttgc acctaatgca
      241 catggtgcat aaatatcaac atctaaagaa aaaatactgt caggagctac aacctctgct
      301 ttataagttg ttgctacttg ctgcaatgca tctttgttga tatccgtaac atatactttg
      361 gcaccttctt ctgtaaggtg tttaactaag ttgcttccaa catgacctgc accctgcaca
      421 cacacttttt taccacttag gctgtcattt ccccacactt cttttgctga agctttcatt
      481 cccatataaa caccataggc tgtcaccggt gatggatcgc cactgccacc cattgactca
      541 ggaagtcctg taacatgctt ggtttctttg gaaatatatt ccatatcttt tgtagatgta
      601 cctacatctt ctgcagtaat atatctcccg cttaatgagt tcacatattg tccaaacttt
      661 ctgaataagg cttctgtttt atcctttttg ctatcaccaa taataactgc ctttccacct
      721 cctagattaa gtcctgaaat agctgctttg taggtcattc cacgcgataa acgaagcaca
      781 tctgtcaagg catcgttttc attctgatac atccacatac gtgttccgcc aagtgcagga
      841 cctaaattgg tattatgaat ggcaataata gcctttaaac cggtttcatg gtcataacaa
      901 aacaccacct gttcgtgttt catctcagta acttgtgcaa aaacactatt ggctgttgct
      961 atggtttctt tcatttcttt tacttcaatc atttacaatg tttttatctg attgttaatt
     1021 cagtttaatt tgcaacccca acgacctgca aacaggtcgc aaaagtagtt tttttttgct
     1081 ctgaacaaaa atttcattta catgtccgat ctgttaacat taaatcgctt tttcgtaaaa
     1141 tacagaggaa ggcttgctct tgggctcatt ttcgtactct tagccaacta tttcggcctt
     1201 ttaccagcac aaataacccg aaaagcaata gattttattt caactggcac acaccataat
     1261 tattccgatt cggccaaaac tttaacattt tatgcattag gcatttttgg agtagtcttt
     1321 ctacgtggcg tcctactgtt tctgatgcgt caaaccatca ttgtcatgtc gaggcacatt
     1381 gaatttgata tgaaaaatga gatttatgat cagtatcaaa aacttgatat gcacttttat
     1441 gcacatgcca atactggcga ccttatgaac agaattagtg aagatgtgag ccgtgtgcgt
     1501 atgtacaccg gtccggctat aatgtactta gtcaatatta ctgtgatgat aattgtggtt
     1561 tttattgcca tgatgaacat cagtccgggc ttaacattag ttgtgctatt tccactgccg
     1621 ctgcttgtta tcctgattta ttacgtgcag aaaatcatca acaccaaaag cgaaaaagtt
     1681 caggaggcat taagtacact ctctaccaac gtacaggaat cttttgcagg aattcggata
     1741 gttaaatctt tttctaaaga aaatgacttt gcagctttat ttcataatca ggcaaaaaat
     1801 tattttacac aaacaatgca actggcacgt atcaatgcat tgtttattcc tgccatcatg
     1861 ttgcttgtag gtattagctc tgttacagta atttattatg gtggaatttt ggttgatgaa
     1921 aagcaaatca cctatggaaa tattgcagag tttgttattt atctcaatat gcttatgtgg
     1981 ccagtaggta tgctggggtg gattattaca cttatccaaa gagcagatgc cagtcagcgc
     2041 agaattaaca gttttttgca tacagagcct gaaattcagt gcaacaacac aggaatagtt
     2101 ccagacaaaa ttaaatccat agaattaaat aatatttcat tttcattcgg aaaaaacaac
     2161 gattctgcat tggaaaatat taatctgcag ctctcaacgg ggaaagtcat agctgtagtt
     2221 ggaacaacag gctccggtaa aactactatt gcaaatctaa tgtcgaggct ctacgatcca
     2281 acacaaggag aaattttggt taacgatatt ccacttaaaa atattaacct tgaatggtgg
     2341 cgcaggcagg ttggcttcat cacgcaggat gtacttttgt tttctgatac cattgccaac
     2401 aatattgcat ttggaataaa ggagtacaat tcagaaacgt tgacagaggc tgcaaaaaca
     2461 gctatggttt atgaggaaat agttcatttt aaagatggct ttaacac
//
LOCUS       DAORQT010000237         2486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8572_length_2486_cov_7.851913, whole genome shotgun sequence.
ACCESSION   DAORQT010000237 DAORQT010000000
VERSION     DAORQT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8572_length_2486_cov_7.851913"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..972
                     /locus_tag="PK134_07475"
     CDS             46..972
                     /locus_tag="PK134_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRB38725.1"
                     /translation="MSMNIAVIDCGTNTFHLIIAGISEGSFKILYRKNLAVKLGEGGI
                     SNNKIARAAFGRGIDALKEFAADMKQFIIDKQLCYATEAIRRAANGKDFVEEAKKQSG
                     ITLEIIDGLREAELIYSGVSHAVPFGKEKVLIMDIGGGSTEFVIAGNGKMLWRKSFPL
                     GAALLLDMFKPSDPIHEVQEKIITEHINSVLFPLTEALKQYGPVNTLVGSAGSFDTFL
                     DLCLHCFHKIKANTTWWPLDSEEYFDVHQILLTSTLQQRLEMPGMIAMRADMIVVASM
                     LLHYVIHKHNMNKLLVSAYALKEGMLYFAMNK"
     gene            1003..2163
                     /locus_tag="PK134_07480"
     CDS             1003..2163
                     /locus_tag="PK134_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB38726.1"
                     /translation="MSRILIIDDEKSIRHSLKEILEYENYKVDEAPDGIEGIKMAEKE
                     KYDIIFCDIKMPRMDGIEVLENLQNKCPEVPVVMISGHGNIDTAVEAIRKGAFDFIAK
                     PLDLNRILITVRNALDRTVLVKETKTLKRKISRTNEMIGESEGIRKIKDMIDRVAPTD
                     ARVMITGSNGTGKELVARWIHEKSSRNNMPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKTRSGKFEQANGGTIFLDEIGDMSLSAQAKVLRALQENRITRVGGEKDFPVDVRII
                     AATNKDLKSEIEKNNFREDLYHRLSVILIHVPALDDRREDIPVLTNYFLKEIADEYKS
                     PAKEIAPDAIEQLQKKNWTGNIRELRNVVERLVILGGKTITKDDVINYVGVK"
     gene            complement(2235..>2486)
                     /locus_tag="PK134_07485"
     CDS             complement(2235..>2486)
                     /locus_tag="PK134_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HRB38727.1"
                     /translation="TTGLLFSYKLGSHTKKVYNKDGKKEKDKTFDDFNIDPFRYDATV
                     RIGYSDFTVFANYALSELFKSGKGPELHPWTIGVSLVPW"
BASE COUNT          851 a          432 c          517 g          686 t
ORIGIN      
        1 aaagcctacc tacaaagaat tgtttcgctg gttgttttat aagtaatgtc aatgaacatt
       61 gctgtaattg actgtggcac caacaccttc catctgatta ttgccggtat ttcagaaggc
      121 agttttaaaa ttctttaccg caaaaactta gctgtaaaac ttggcgaagg cggcatcagc
      181 aataataaaa tagcacgtgc agccttcggt cgtggcattg acgcacttaa agaatttgct
      241 gccgacatga aacaatttat cattgacaaa caattgtgtt atgcaactga agccatacgc
      301 agagcagcca atggaaaaga ttttgttgaa gaagcaaaaa aacaatcagg cattacactt
      361 gaaattattg atggcttgcg cgaagcagaa ctgatatact caggagtaag tcatgctgtt
      421 ccttttggaa aagaaaaagt actgataatg gatattggtg gaggaagtac agaatttgta
      481 attgcaggaa acggaaaaat gttgtggcgg aaaagttttc ctttgggtgc tgccctgttg
      541 ttagatatgt ttaaaccatc agacccgatt catgaagtgc aggaaaaaat aattactgaa
      601 catatcaact cagttttatt tccacttaca gaagcattaa aacaatatgg tccggtaaat
      661 acattggtag gatcggcagg ttctttcgat acctttctgg atttatgtct tcattgtttt
      721 cataaaataa aagcaaacac tacctggtgg ccattggact cagaggaata ttttgatgta
      781 catcaaattt tgttaacctc aaccttacaa caacgacttg aaatgccggg aatgattgcc
      841 atgcgtgccg atatgattgt tgttgcatct atgttacttc actatgttat tcacaaacac
      901 aatatgaata aacttttagt gagtgcttat gcattaaagg aaggaatgct ttattttgca
      961 atgaataaat aacaacagca aactgaccgt aaacaaaaga gcatgtcacg tatattaatt
     1021 atagatgatg agaaaagcat ccgccattcg ctgaaagaga ttcttgaata cgagaattac
     1081 aaagtggatg aagcgcccga tggtattgaa ggcattaaga tggctgagaa agaaaaatat
     1141 gatattattt tttgtgacat aaaaatgcca cgcatggatg gcattgaagt gttggagaat
     1201 ttacaaaata aatgtcctga agtgcctgtg gtgatgatat cgggacatgg caacattgac
     1261 actgctgttg aagccattcg taaaggtgct tttgatttta ttgcaaagcc gcttgaccta
     1321 aatcgtattc tcattactgt tcgcaatgca ctcgaccgta cagtactggt aaaagaaacc
     1381 aagacactga aaagaaaaat ttcgcgtacc aatgaaatga ttggtgaaag tgaaggcatt
     1441 cgtaaaataa aagacatgat tgaccgtgtg gcacctaccg atgcacgcgt gatgattaca
     1501 ggaagcaatg gaacaggaaa agaattggtg gcacgatgga tacatgaaaa gagcagcaga
     1561 aacaacatgc cactgataga agttaattgc gcagcaatac catcagagct aatagaaagc
     1621 gaattgtttg gtcatgaaaa aggcgcattt acctctgcca taaaaacacg aagcggaaaa
     1681 tttgaacaag caaacggtgg cactattttt cttgatgaga ttggcgacat gagcctgtcg
     1741 gcacaagcca aagtgttgag agcattgcag gaaaacagaa ttacaagagt aggtggcgaa
     1801 aaagattttc cggttgatgt acgtattatt gctgctacca acaaagattt aaaatcagaa
     1861 atagaaaaaa ataatttcag agaagattta tatcatcgtt tgagtgttat actcattcat
     1921 gtgcctgcat tggacgaccg cagagaagat attccggttc tgacaaatta ttttctgaaa
     1981 gaaattgctg atgaatataa aagccctgca aaagaaattg ctccggatgc aatagagcag
     2041 ttgcagaaaa aaaactggac aggaaatatt cgtgagctgc gtaatgtggt agagcgtttg
     2101 gtaattctcg gtggcaaaac catcactaag gatgatgtta taaactatgt aggcgtgaaa
     2161 taattcacgc ccacataatt catttatcaa ttctgattac aacctacttt gtcaactcga
     2221 cacaggcttt agtactacca aggtactagt gaaacaccaa tagtccaggg atgcaactcc
     2281 ggcccctttc cactcttgaa aagttctgac agtgcatagt ttgcaaatac agtaaaatca
     2341 ctgtagccta tgcgaactgt tgcatcataa cggaagggat caatattaaa atcatcaaaa
     2401 gttttatcct tctctttttt accatcttta ttgtaaacct ttttagtatg tgagcccaat
     2461 ttgtaactaa acaacaaacc ggtagt
//
LOCUS       DAORQT010000238         2485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8576_length_2485_cov_7.346502, whole genome shotgun sequence.
ACCESSION   DAORQT010000238 DAORQT010000000
VERSION     DAORQT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8576_length_2485_cov_7.346502"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PK134_07490"
     CDS             complement(<1..1023)
                     /locus_tag="PK134_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38728.1"
                     /translation="MYLLFAVQLLQAQSPTAYERAGDKAMKEGDYYSAFAFYFNSYAS
                     NNTDLVITWKLAEACRLYNDYENAQKYYNKAYGLDKQGQFPLIAFYLAEMNQRQGYYE
                     EAASIYNNYLQMHGRDNSYFTDKAKRELANCQNIKQLLKDTAKVTIQNSGTEINSVYS
                     DFAPSYLDSTTLLYSSLRFENKDKKTQKKNKYISKLLVSNNERNTWSRPDNYSTQLNR
                     PGLHSCNSAVSGNGKLLVYTQCQRERDKLICTLYYSTQKDGNWSDPKPLNDSINFKGY
                     TATMPAIGNDELTGYVLYFVSDRPGGLGKADIWKCSITPDFVFGNPVNVGAPVNTVDD
                     DITPFYD"
     gene            complement(1074..2084)
                     /locus_tag="PK134_07495"
     CDS             complement(1074..2084)
                     /locus_tag="PK134_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRB38729.1"
                     /translation="MKNLLSGIFTICLFCLNAAGQDVHFSQFDFSPLHQSAAQTGNFK
                     GDYRLTAINRVQYKSVTVPYKSLGASVDMNIAKTDEKKSWWSAGLLFENDVTGDADYK
                     TTIVDVSVAWNSFLDENKKHQLTLAIQPGWMQNGLNFSELYFGSQYDGDIFNPNLPTG
                     ESPARSSYSNFNLNAGAGYKYAFSENSDLNLIFGASHLNQPDISFLNDKAKLFTRLTG
                     VGVLNLMLSEKISLQPQVLFYRQGKFSQLNGGANVQLHSLENNFKNFRFSAGVFYRNK
                     DAVIARIGAGIGNLDAGFAYDFNTSDLKRASNGRGAYEVALSYIITKVKPLKKNPPCP
                     VY"
     gene            complement(2088..>2485)
                     /locus_tag="PK134_07500"
     CDS             complement(2088..>2485)
                     /locus_tag="PK134_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB38730.1"
                     /translation="IANPVSTPPVDIEYILSITDAFGCKINDTAKIVVIQYRCEEPEI
                     YIPNSFSPNGDRVNDEIYVYGGQIKELLFRIYDRWGEKVFETKQPGQGWDGFYRGKRV
                     TPGVFVYYVEATCYDNQKFFKKGNITVIR"
BASE COUNT          773 a          505 c          413 g          794 t
ORIGIN      
        1 gtcataaaat ggagtgatgt catcatcaac agtattcaca ggtgcaccaa cattaacagg
       61 atttccaaaa acaaaatcag gagtaataga acatttccag atgtctgcct tacccaaacc
      121 accgggacgg tcgcttacaa aataaagcac atagcctgtc aattcatcat ttccaattgc
      181 aggcattgta gctgtatatc ctttaaaatt aatgctgtca ttgagtggtt tcgggtcaga
      241 ccaattgcca tctttctgtg tactgtaata taacgtacat atcaatttat ctctttcacg
      301 ttgacattgc gtatatacta acaatttacc attaccactg acagccgaat tacatgaatg
      361 caatccgggc cgattgagtt gtgtgctgta attatcagga cgactccatg tattgcgttc
      421 attattgctg acaagcagtt tagatatata cttatttttt ttctgtgtct ttttgtcttt
      481 attttcaaat cgcagcgatg agtacaacaa ggttgtactg tctaaatatg acggagcaaa
      541 atcagaataa acagaattta tttcagtgcc tgagttttga attgtaacct ttgctgtatc
      601 tttcaacaac tgtttaatat tttggcaatt ggctaattcg cgttttgctt tgtctgtaaa
      661 ataactgtta tcacgaccat gcatttgtaa atagttattg tagattgaag cagcttcctc
      721 ataataaccc tgtcgctgat tcatctctgc cagataaaat gcaattaatg gaaactgacc
      781 ttgtttatct aatccataag ccttgttata atacttctgt gcattttcat aatcattgta
      841 aagccggcat gcttctgcca gtttccaggt tataactaaa tctgtattat tgcttgcata
      901 agaattaaaa tagaatgcga atgcagaata ataatcacct tctttcatag ccttatcacc
      961 tgcacgctcg taggctgttg gagattgcgc ctgcaacaac tgtacagcaa ataacaagta
     1021 cattaacatt acaatctttg caacaacaat cctcctgctg ccgaaaaaat atatcaataa
     1081 acggggcatg gtggattctt ctttaatggt tttactttgg tgatgatgta agataatgct
     1141 acttcgtaag cgcctctgcc gttacttgcc cttttcaggt cagatgtatt gaaatcgtat
     1201 gcaaaaccgg catctaaatt gccaatacct gctccaatac gtgcaatgac agcatctttg
     1261 ttacgataaa atactcctgc cgagaatctg aaatttttaa aattattctc taatgaatgt
     1321 agttgaacat ttgcaccgcc atttaactgt gagaattttc cctgacgata aaataacact
     1381 tgtggttgca gagaaatttt ttcggaaagc atcaggttta aaactccaac acctgtgagt
     1441 cgggtaaata attttgcttt atcattcaga aatgaaatat caggttgatt caggtggctg
     1501 gcaccgaaaa tcaaattcaa atcagaattt tcactaaatg catatttata accggcacct
     1561 gcattcaaat taaaattgga atagctactg cgcgctgggc tttcacctgt aggcaggtta
     1621 ggattaaaga tgtcaccatc atattgcgaa ccgaaataca gttccgaaaa attcaaacca
     1681 ttctgcatcc accccggctg aatagctaag gtgagctgat gttttttgtt ttcatctaaa
     1741 aaagaattcc aggcaacaga aacatcaaca atagtagttt tataatctgc atcacctgta
     1801 acgtcatttt caaataataa acctgcactc caccatgatt ttttttcatc agtttttgca
     1861 atgttcatat caacagatgc acctaatgat ttataaggaa ctgtaacaga tttatactgt
     1921 acacgattaa ttgcagtcag tctgtaatcg cctttaaagt ttccggtttg tgctgcactc
     1981 tgatgcaatg gcgaaaaatc aaactgcgaa aaatgcacat cctgccctgc tgcattcaaa
     2041 cagaacaaac aaattgtaaa tatgcccgac aggagatttt tcatttctta ccgtattacg
     2101 gttatattac cttttttaaa gaatttctga ttgtcatagc aagtagcctc aacatagtaa
     2161 acaaacacac cgggtgttac acgtttgcct ctataaaatc catcccaacc ctgacccggc
     2221 tgtttggttt caaatacttt ctctccccaa cgatcgtaaa tacgaaatag caactctttt
     2281 atttgtccgc cataaacata aatctcatca ttaactctgt caccattcgg gctaaatgaa
     2341 ttaggaatat aaatttcagg ttcttcacaa cgatattgta tgacaacaat ttttgcagtg
     2401 tcattaatct tacaaccgaa tgcatctgtt atgctaagga tatactcaat atccacagga
     2461 ggtgtgctga caggatttgc aatac
//
LOCUS       DAORQT010000239         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8627_length_2475_cov_7.955785, whole genome shotgun sequence.
ACCESSION   DAORQT010000239 DAORQT010000000
VERSION     DAORQT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_2475_cov_7.955785"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..412
                     /locus_tag="PK134_07505"
     CDS             89..412
                     /locus_tag="PK134_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRB38731.1"
                     /translation="MRFQKHIFVCTNQRKEGERKCCGEVHGLALVAAFKKKIKELQLP
                     VPIRAQRSGCLDACDYGPSVVVYPEGIFYGNVQLQDVDEIVEQHIKNNKPVERLIINF
                     DDKKV"
     gene            complement(426..779)
                     /locus_tag="PK134_07510"
     CDS             complement(426..779)
                     /locus_tag="PK134_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRB38732.1"
                     /translation="MDIEKFRNYCLSKQETTESFPFDEDTLVFKVAGKMFALVSLSEK
                     PMTVNLKMDPEIISEWRDRYSEITPGFHMNKQHWNSVIFNGSIPLAELKWLIDHSYSE
                     VVKKLPKKVRERFTI"
     gene            complement(864..2327)
                     /locus_tag="PK134_07515"
     CDS             complement(864..2327)
                     /locus_tag="PK134_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38733.1"
                     /translation="MDILRKTIKKLSDEEYQALLLQVSGKKKNKPYYVLEATRNNNHF
                     DDTEMMKQLQVNPSAYYTLKSRLNTRIAEILSKKVENPIRELMEEVVRVPATLYGTNR
                     DFSIRALKEIEKQLKEYDMSNELITVYKTLALLHIHHEDYEHYNKLYNKHVAYALAIS
                     KAEQMFFLFFKKLGTYQLTLDPDTLEEVIMLKRKIANIAEMYDSHRLFVLYNIVRFYY
                     LCSLPSKRQELKSLELEIESILKRINEIFNAFPIDTLYSNISPLVDILHYEYYQKIQN
                     QVRADHYLSNGINDTIIDWSEKTAMHFFIIQYLNSRVEKFLADGEMYRLNEFNQTIEK
                     TFDLNINEPYHYISFKRYIALCKFYSKDYQGAAKTIYDLRNNISLKQFTHTDVDSKLF
                     QGLCYCLMGEDSLAQQMISSIKRQIADFEHEFESTTIFIKILKTALKPSDFRRKIVRI
                     NELLIKFEEANNGDKPILKYIKIDDALIRKMTNPIKD"
BASE COUNT          793 a          412 c          404 g          866 t
ORIGIN      
        1 ctgtaatatt taacagcaaa attaagcatt gaatttgaat tggactaata gagtttgaat
       61 tctgaacaca attatcttaa tttgcattat gaggtttcaa aaacatattt ttgtttgtac
      121 taatcagcgt aaagaaggtg aacgtaaatg ctgtggtgaa gtacatggtc ttgcattggt
      181 tgcagcattt aagaaaaaaa ttaaagaatt acagttgccg gtgccaattc gtgcacaaag
      241 gtcaggttgt ttagatgcct gcgactatgg tccatcagta gttgtttatc ccgaaggtat
      301 tttttatggt aatgttcagc tacaggatgt tgatgaaata gtagaacaac atataaaaaa
      361 caacaaacct gttgagcggc taattataaa ttttgatgat aagaaagtgt aatttctgaa
      421 taacactaaa ttgtaaatcg ctctcttact tttttgggta attttttaac tacttcagaa
      481 tagctgtgat caataagcca ttttaattct gcaagaggaa tggagccatt aaaaataaca
      541 gaattccaat gttgcttatt catgtgaaat cccggtgtaa tttcggaata gcgatcacgc
      601 cattctgata taatttcagg atccattttg aggtttaccg tcattggttt ttctgataac
      661 gaaactaaag caaacatttt tccggcaacc ttaaacacaa gtgtgtcttc gtcaaaagga
      721 aaactctctg ttgtttcttg cttcgacaaa caatagttcc tgaatttttc aatatccatt
      781 tgatagtatc agatgataaa agtaacagaa atttgtcaga aaaagcacaa aaataaaagg
      841 cggccatttc tgccgccttt gttttaatct ttaatcggat ttgtcatttt ccgaatcaat
      901 gcatcatcaa ttttgatata cttcagaatt ggtttatcac cattatttgc ttcttcaaac
      961 ttaatcagca actcgttgat gcgcacaatt tttcttctga agtcagaagg ctttaatgct
     1021 gttttaagaa tcttaataaa tattgttgtt gactcaaact catgttcaaa gtcggcaatc
     1081 tgacgtttaa tacttgaaat catttgttgt gcaaggctat cttcacccat caggcaataa
     1141 cacaaaccct gaaagagttt gctgtcaaca tcggtatgag tgaactgctt caaagaaata
     1201 ttatttctta aatcgtatat agtttttgct gcaccctgat aatctttact ataaaattta
     1261 cataaggcaa tgtatcgctt aaaagagata taatgataag gctcgttaat attgaggtca
     1321 aaagtttttt caattgtctg attgaattca ttcaaccggt acatttcacc atcagcaaga
     1381 aatttttcaa ctcgcgaatt cagatactga atgatgaaaa agtgcatagc tgttttctca
     1441 ctccagtcaa taatagtatc gttaattcca ttactaaggt aatgatctgc tctaacctga
     1501 ttctgtattt tctgatagta ctcatagtga agtatatcta ccaacggact aatgttgcta
     1561 taaagtgtgt caatcggaaa tgcattaaat atttcattga tgcgttttaa aatactttct
     1621 atttcaagct caagggattt taactcctgt cgttttgatg gcaatgaaca cagataatag
     1681 aatctgacaa tattgtataa cacaaacaaa cgatgcgaat catacatttc tgcaatattg
     1741 gcaatctttc ttttaagcat tatgacttct tccaacgtat caggatcaag tgttaactga
     1801 taggttccta attttttaaa gaacagaaaa aacatttgct cggcttttga aatagccaat
     1861 gcataagcaa catgtttgtt ataaagtttg ttataatgtt cgtagtcttc atgatgaatg
     1921 tgtaacaatg caagagtttt ataaactgta atcaactcat tgctcatgtc atattcctta
     1981 agctgctttt caatttcttt taatgctctg atagagaaat cgcggttggt tccataaagt
     2041 gtagccggaa cacgaaccac ttcttccatc aactcgcgaa ttgggttttc aactttcttg
     2101 gaaagaattt ctgctattct tgtattgagg cgggatttta atgtataata agcacttgga
     2161 ttaacctgaa gctgcttcat catttccgta tcatcaaaat gattgttatt gcgtgtagct
     2221 tctaagacat aataaggctt gtttttcttc ttgcccgaga cctgaagcaa caatgcctga
     2281 tactcttcat cactaagttt cttaattgtt tttcttaata tatccatata agcttatgat
     2341 ttatttttta aagactcttc cttgttacgt aaaaaaaact gcaaggtttt agctttttta
     2401 aaaaatttcc gacttagcta attactctaa ttgcataatt gaaactcaat attaatccat
     2461 ggcatatatg taata
//
LOCUS       DAORQT010000240         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8634_length_2474_cov_4.875982, whole genome shotgun sequence.
ACCESSION   DAORQT010000240 DAORQT010000000
VERSION     DAORQT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8634_length_2474_cov_4.875982"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK134_07520"
     CDS             <1..214
                     /locus_tag="PK134_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRB38734.1"
                     /translation="DMIMLKMAVTEVLKFDQIPVKVTLNEYIDISKDYSTPQSKVFIN
                     GVLDKIVEELRKQDKVKKTGRGLVEN"
     gene            complement(436..1080)
                     /locus_tag="PK134_07525"
     CDS             complement(436..1080)
                     /locus_tag="PK134_07525"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRB38735.1"
                     /translation="MNSKLIIFIVISAVHLVVFITKPLLMPILLWYFTEGLSFGKLTL
                     IQKRMITALTLAWIGDIVNLYSQINHWIFMAGVIIFLAMQIMYIIICFYLIRFKQINQ
                     LYFIFSTLLFIAYGSALLWILLPHLNELTIPVLIYASALTLTGIASALTKDQLPRNVF
                     YCLLTGTLLYMTTDSLDAINNFKASFPYAAFWIALTYLSAQMLITIAVKKIKTI"
     gene            complement(1240..2040)
                     /gene="kdsA"
                     /locus_tag="PK134_07530"
     CDS             complement(1240..2040)
                     /gene="kdsA"
                     /locus_tag="PK134_07530"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRB38736.1"
                     /translation="MPQLKHTASENFFLMAGPCIVESEQMCMDIAGTVKEITDRLQIP
                     YIFKASFRKANRSRLDSFTGIGDVPSLEILRKVRERYQLPVVTDIHESHEAALAADYV
                     DVLQIPAFLCRQTELLVAAARTGKTVNVKKGQFLSPESMKFAIDKIRQSGNNQVMVTE
                     RGTMLGYGDLVVDMRGIPEMKKFNVPVVMDVTHSLQQPNQSSGVTGGKPELISTIARA
                     AMAAGADGIFIETHPNPAKALSDGANMLRLDLLEKLLVSLLKVQQAVR"
     gene            complement(2084..>2474)
                     /locus_tag="PK134_07535"
     CDS             complement(2084..>2474)
                     /locus_tag="PK134_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRB38737.1"
                     /translation="VQLNDAINHQFFCNDVFTFLKETKSTYQVWVVDPPAFAKRLDAV
                     RNAMIGYRNLNTAVFRKAPAGSIIFTFSCSQAVDALTFRKVIFQSAKEAQRQIKILHI
                     LSQPADHPVSVFHPEGEYLKGLVLYVE"
BASE COUNT          802 a          445 c          469 g          758 t
ORIGIN      
        1 cgatatgatt atgcttaaaa tggctgttac agaagtgttg aaatttgatc agatacctgt
       61 aaaagttacc ctcaacgaat atattgatat ttctaaagat tacagtacac cacagagcaa
      121 ggtatttatt aatggcgttt tggataaaat tgttgaagag ctacgtaagc aagataaagt
      181 aaagaaaacc ggacgaggcc ttgtagagaa ttgataattc tgaatgtaga gttcagaatt
      241 atcaattgaa aagagttttt ggtggagaga ttgcacttct atgcttcatg ctaaagccta
      301 agtttttttt catcacctaa tttcacctaa tttcaaataa ttgcctcatc acataaatga
      361 attcggaatt atcaaattat ctgattcact aggctccttc acacttcatc acttttcact
      421 ccacgcttca tcactctaaa ttgtttttat tttctttact gcaattgtaa tcaacatttg
      481 tgctgaaaga taggtgagtg caatccagaa agctgcataa gggaaagagg ctttaaaatt
      541 attgatggca tccaaactgt cagtagtcat gtaaagtaat gtgcctgtca gtaaacaata
      601 aaaaacattt cgaggtagtt gatcttttgt caatgctgaa gcaattccgg ttaatgtaag
      661 agcagatgca taaattaata ccggaatggt aagctcattc agatgtggca gtaatatcca
      721 gagcaacgca ctgccgtaag caataaataa taaggtgctg aagatgaaat atagctgatt
      781 gatttgttta aagcgaataa gataaaagca aataatgata tacataatct gcattgcaag
      841 aaaaataatt acacctgcca taaatatcca atgattgatt tgactgtaaa gatttacaat
      901 gtcgccaatc catgccaaag tgagcgctgt aatcatgcgt ttttgaatga gtgttaattt
      961 gccaaaactt aaaccttctg taaaatacca aagcaatatg ggcatcaata atggcttggt
     1021 gataaataca acaagatgaa ctgctgatat aacaataaat ataatcagct tgctgttcat
     1081 tgtatctgtt ttgatatgat cattgtttta ctttcacttg ccactttttc ttaatctatt
     1141 gtataaatgg cgatgtaaag tatgaaataa aaatacaatt gtccaaatct aagattggaa
     1201 aaaagacaaa aagataatgt gttatgcagt catatcacat cagcgaacag cctgctgcac
     1261 ttttagtaat gaaactaaaa gtttttcaag caggtcgaga cgtagcatat tggcaccatc
     1321 actcaatgct ttggccgggt ttggatgcgt ttcaataaaa attccatcgg caccggcagc
     1381 cattgcagca cgggcaatag tgctgataag ttcaggttta cctcctgtta caccggatga
     1441 ctgattgggc tgttgcaatg aatgagtaac gtccattacg accggtacat taaatttttt
     1501 catttcagga ataccacgca tgtcaaccac taaatctcca tagccaagca tggtgccgcg
     1561 ctcggtaacc attacctgat tgttgcctga ttgtcgaatt ttatctatgg caaacttcat
     1621 agattcggga gaaagaaatt gtcctttttt tacgttaaca gtttttccag ttcgtgctgc
     1681 tgccaccaac aattctgtct ggcgacaaag aaatgctgga atctgtagta catcaacata
     1741 atctgctgca agtgcagcct cgtggctttc gtgaatatcg gtaacaacag gtagctgata
     1801 acgctcccgg actttacgca gtatttcaag agaaggaaca tcgccaatgc cggtaaatga
     1861 atcaagtctt gagcggttgg cttttctgaa acttgcttta aagatatatg gaatctgaag
     1921 tctgtcagta atttctttca ctgttcctgc aatgtccata cacatctgtt ccgattcaac
     1981 aatacatggt ccggccataa gaaaaaaatt ttctgaagct gtatgtttta actgtggtat
     2041 attctcaagc atattgactt aatgtgatta aattaaaatg gtgctattct acatacaaaa
     2101 ctaaaccttt taggtattcg ccttcgggat gaaaaactga aacaggatgg tcggcaggtt
     2161 gagaaagtat gtgcaggatt tttatctgcc tttgtgcttc ttttgcactc tgaaaaatta
     2221 ctttcctgaa ggttaatgca tcaacagcct gcgagcaaga gaaggtgaaa atgatacttc
     2281 ctgcaggtgc tttgcgaaaa acagcagtgt tcagattccg atagcctatc atagcatttc
     2341 taacagcatc aagccgcttg gcaaatgcag gtgggtcaac tacccaaacc tgataagtgc
     2401 tttttgtttc tttcagaaat gtaaatacat cgttacaaaa aaattgatga ttaatagcat
     2461 cgtttaactg aaca
//
LOCUS       DAORQT010000241         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8752_length_2454_cov_8.829512, whole genome shotgun sequence.
ACCESSION   DAORQT010000241 DAORQT010000000
VERSION     DAORQT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8752_length_2454_cov_8.829512"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..2283)
                     /locus_tag="PK134_07540"
     CDS             complement(40..2283)
                     /locus_tag="PK134_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38738.1"
                     /translation="MVFSAIVLEWNMHKRDLDFIRITVFAKKTLHEEENRANKYVEWL
                     GNMLSRQTSYSAIDDSLLLTSQKPVSAFYSFVYLNDSLIYWDNNAVSFSYNQVKNGIN
                     QQLIQLKNGWYELFRYQRRNITIIGLLPIRAGYDFQNKYLRNEFNPVLGIPEHALLLP
                     SPATGSYPVISSDGNYLFSIKYNPIMSDPGRLWYIAALYLAGFFLLLISFYLYARQWL
                     LKNPLVSFVIISLLVFIKWLMLTFRFPEFLYGMPLFNPKFYATSYFLNSLGDFLLSAS
                     VFCCVILFVYNYLHLRRRKIKVIRQSPTLLSIVVIGNLLFTFLFSVFINYLISGLILN
                     SKISFNVNNVFELTFFSLIGFLIIGILLFSFYVSCEGTVKFAEYSGFSLPYNFLLFLI
                     TQGLFLVVLILFRNTDVFVNYGVATFFLTNSLILFISYIRFTSRQNFSFVRYMFVIAG
                     FSVYAAYTISSFNMVREKNNMRLLVSKVENREDLIAEYLFQEIDQKLKTDPVITSNII
                     QGRSPLLEEKIKKRITLNAFNQAYWTRYDIQIKAYDSAGVSLFANSDTLRNVTFYDSI
                     INTQTRPTYSHSFNYLSSNSGKIGYIGKITYPKNASGELAATIIIQLDSKFNQEEGGF
                     PDLLLSEKMPASRDLSKYAYAKYENGKLIFQSGKFNYFHSAATYDRMFGLVKSDQFVS
                     LGGFIHLFYNQDKNNLIVISYKTPQAIEQVTLFSYVFIFFSATFILIYMIWLFLANNF
                     RLHLYSQ"
BASE COUNT          900 a          403 c          428 g          723 t
ORIGIN      
        1 aaaaaatcgg tttattggaa tatacttcag cctacgaaac tattgtgaat aaagatgcaa
       61 cctgaaattg ttggctaaga aaagccaaat catgtaaatt aaaatgaatg ttgcagaaaa
      121 gaaaataaac acataagaga ataaagtcac ctgttcaata gcctgaggtg ttttatatga
      181 aattacaatc agattatttt tatcctgatt atagaataag tgaataaacc ctcctaatga
      241 tacaaactga tcggatttta ccaaaccaaa catacggtcg tatgtagcag cagaatgaaa
      301 atagttgaac ttgccacttt gaaaaatcag tttgccattc tcatattttg cataagcata
      361 ttttgaaaga tcgcgcgatg caggcatttt ttctgaaaga agtaaatcgg gaaacccacc
      421 ttcttcctga ttaaatttag agtcaagctg aataattatt gtagcagcaa gttcaccgct
      481 ggcatttttt ggatatgtaa tttttccgat gtatccaatt ttacctgagt tagaactcag
      541 gtaattaaag gaatggctat aggttggtcg tgtctgcgta ttgatgatgc tatcataaaa
      601 agttacattg cgcaatgtgt cggaattggc aaacagagaa acacctgcag agtcataagc
      661 tttgatttga atatcgtagc gtgtccaata tgcctgatta aatgcattta gagtgatacg
      721 cttttttatt ttttcttcta acaagggcga ccttccctga ataatatttg aagtaattac
      781 aggatcagtc tttagtttct gatctatttc ctgaaataaa tattctgcaa tcaagtcttc
      841 tctgttttca acctttgaaa ctaacagacg catgttgttt ttttcgcgca ccatattaaa
      901 gctcgaaatg gtatatgcag cataaacaga aaagcctgct atgacaaaca tgtagcgtac
      961 aaatgaaaaa ttttgccggc ttgtaaaacg aatgtaactg atgaagagaa tgagcgaatt
     1021 ggtcagaaag aatgtggcta caccataatt cacaaaaaca tcagtgtttc gaaacagaat
     1081 taatacaacc agaaaaagtc cttgtgtaat taaaaataaa agaaagttat aaggtaatga
     1141 gaaaccacta tactcagcaa attttactgt cccttcgcag gaaacataaa acgaaaaaag
     1201 cagaatacct attattaaaa atccaatgag gctgaaaaag gttaattcaa aaacattgtt
     1261 tacgttgaaa gaaatttttg agttaagaat taatccgctt ataagataat tgataaaaac
     1321 agaaaacaag aaagtaaaca acagatttcc gattacaaca attgaaagta atgttggact
     1381 ttgtctgata acttttattt ttcttcttct taaatgaagg taattgtaaa cgaacagaat
     1441 gacacaacaa aaaaccgatg cactaagcaa aaaatcaccc aatgaattca gaaagtatga
     1501 tgttgcataa aatttaggat taaatagcgg catgccataa agaaattcag gaaaacggaa
     1561 tgtcagcatg agccatttga tgaaaactaa aagactaatt attacaaacg acactaatgg
     1621 attttttaat aaccactgtc ttgcatagag atagaatgaa attaataaaa ggaaaaatcc
     1681 ggctaaatac aatgcagcaa tgtaccacaa gcgacccgga tcgctcatta tcggattgta
     1741 ttttatactg aataaataat ttccatcaga gcttattacc ggataagatc ctgttgccgg
     1801 tgagggtaac agaagggcat gttctggaat tcccaaaaca ggattaaact cattgcgcaa
     1861 atacttattt tgaaaatcat agcctgcacg tatgggcaac aaaccaatga tggtaatatt
     1921 tcttcgttgg taacgaaaca attcatacca accatttttt agttgaataa gttgttggtt
     1981 gattccgttt ttaacttgat tgtatgagaa acttacagca ttattatccc aataaattaa
     2041 tgagtcgttg agatatacaa agctgtagaa agcactaaca ggtttttgcg aagtaagcaa
     2101 cagactgtcg tcaatggcag aataacttgt ttggcgcgaa agcatattac caagccattc
     2161 aacatatttg tttgcccggt tttcttcttc atgtaaagtc ttttttgcaa aaactgttat
     2221 cctgataaag tcaagatcac gtttgtgcat attccactct aatacaatgg cggaaaatac
     2281 cattgccaaa gaaagaataa gtagaaaata cctgaagttg cctttgatca catcccgtaa
     2341 ggttaaacta aacaaaagta tttaatcata cggaaaatca tcaaaatagt tatggtaata
     2401 agccctccaa ggcttatttt tcaggaaagc ctctgctgcg cataagtgct ttaa
//
LOCUS       DAORQT010000242         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8760_length_2453_cov_3.926188, whole genome shotgun sequence.
ACCESSION   DAORQT010000242 DAORQT010000000
VERSION     DAORQT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_2453_cov_3.926188"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PK134_07545"
     CDS             complement(<1..166)
                     /locus_tag="PK134_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRB38739.1"
                     /translation="MKFINTTQAPAPIGPYSQAVQCGNILFVSGQIAIDPANGQLITS
                     SISAQTTRIMQ"
     assembly_gap    446..455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <456..1983
                     /locus_tag="PK134_07550"
     CDS             <456..1983
                     /locus_tag="PK134_07550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRB38740.1"
                     /translation="ISTSGKTNTLIASNNSLQLTELQPMTTFMVSLKSICGNDTSSVS
                     NVVSFTTKHVNVKDSIVRGAYLQMLTDNSVVIRWRTFVSENSRVRFGETMQYGKVKED
                     TANVNEHTVLLSGLLPNTKYFYTIGSTSNDKQGDEENYFITAPKTGTIQPVRIWSIGD
                     FGNGSLAQREVRDSYLRYSKNHPADLWIWLGDNAYSYGYDAQFTYKVFNLYKSIFKYL
                     PLFPSIGNHDYAKKGYLDSASLGTNFPYFKIFDCPENGECGGVPSKTEKYYSYNYSNI
                     HFIVLDSYGAHSDKESDMYKWLSADLKANNARWTICYFHHPPYSRGTHNSDKPGESTD
                     IRQNIIPLLEKYKVDLVMSGHAHDYERTYLLHGHYGKQVTLTQDMIVDKSDGNSPYYL
                     KSAPDYKGTVYVVNGVGGQGGNVKTQGDWPHKAMYSYSKSHHGSMIIDVNKDTLSAIF
                     LTSTDTIFDRFKIVKSDKIKKKNSTKEGFNQNQIKQDDKTDLGFIPIRKEDDYFTLEK
                     "
     gene            complement(2033..>2453)
                     /locus_tag="PK134_07555"
     CDS             complement(2033..>2453)
                     /locus_tag="PK134_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38741.1"
                     /translation="NYGVQTSVRYLIAFRQWIMENTSGVVFVQIRDNNKYEQSKMRTI
                     RSLWEKVMSPFKNLSSNFIVMQDYVNDSFSEYLKTLYGRQIRFVDLQMNQKEERISLS
                     WHLTQKEKLYVIQEGNNELNKFSIEAFCNLLKDEKSW"
BASE COUNT          820 a          439 c          406 g          778 t
ORIGIN      
        1 tttgcatgat gcgcgtagtc tgagcagaaa ttgatgaagt aatcagttgt ccgtttgcag
       61 gatctattgc aatttgtcct gacacaaaaa ggatattacc gcattgtact gcctgactat
      121 aaggtccaat cggagcgggt gcttgagttg tatttataaa tttcatattg caaaagtatg
      181 aaacgatgat aattcaatta agccaactga taaaagtcag cattataata tcgttcttat
      241 ctaactttat ggcctcttat attaaattta gttatgcgtt gttttctgaa atcatttatt
      301 ttacttctac ttatttactt tcctattcaa actatagcat cagattgtaa gcctccggaa
      361 catattacaa taacagatgt tacatcttct tcagcacaag tttcatggaa tgcagttaag
      421 tcaggttcat catttctatt gcaaannnnn nnnnntattt ctacatcagg aaaaactaac
      481 acattaattg cctcaaacaa cagtttacaa cttaccgagc tacaaccaat gacaactttc
      541 atggtttcac tcaagtccat ttgcggtaat gatactagta gtgtaagtaa tgtagtttca
      601 tttacaacca aacatgtcaa tgttaaagat tctattgtga gaggtgcgta tctgcaaatg
      661 cttacagata acagtgtggt tattcgatgg agaacttttg tttctgaaaa cagcagagta
      721 cgctttggcg aaaccatgca atacggaaaa gtaaaagaag atactgcaaa cgttaatgaa
      781 catactgttt tgttatcagg acttctacct aacacaaaat atttttacac cattggctcc
      841 acaagcaatg ataaacaggg cgatgaagaa aattatttta ttacagcacc aaaaacagga
      901 accattcaac cggtaagaat atggtctatt ggcgactttg gcaatggcag tcttgcacaa
      961 cgcgaagtgc gtgattctta tttaagatat tctaaaaatc atccggcaga cttatggatt
     1021 tggcttggcg acaatgcata ttcctatggt tatgatgcac aatttaccta caaagttttc
     1081 aatctatata aatcaatctt taaatatctt cccttgtttc cttcaatagg caatcatgac
     1141 tatgctaaga aaggatatct agatagtgct tcgctcggaa ctaattttcc atactttaaa
     1201 atttttgatt gtcccgaaaa tggtgaatgt ggtggtgtgc caagtaagac agaaaaatat
     1261 tactcctaca attattcaaa cattcacttc atcgttttag acagttatgg tgcacattca
     1321 gataaagaaa gtgacatgta taaatggctt agtgcagatt tgaaagcaaa caatgcacga
     1381 tggaccattt gttattttca tcatccacct tatagtcgtg gaacacacaa ttctgataaa
     1441 cccggtgagt caacagatat tcgtcaaaat attattcctt tacttgaaaa atataaagtt
     1501 gatttggtaa tgagtggtca tgcacatgat tatgaacgca cctatttatt acatggacat
     1561 tatggcaagc aagtaaccct aacacaagac atgatagttg ataagagtga tggcaattca
     1621 ccatactatc ttaaatctgc tcctgactac aaaggcactg tctatgttgt gaatggtgtt
     1681 ggcggacaag gcggtaatgt aaaaacacaa ggtgactggc cacataaagc tatgtattct
     1741 tattcaaaaa gtcatcatgg ctcaatgatt attgatgtga ataaagacac cttatcagca
     1801 atattcctca catcaaccga tacaattttt gatcggttta aaattgtaaa atccgataaa
     1861 atcaaaaaga aaaacagcac caaggaagga tttaatcaaa atcaaattaa gcaagatgat
     1921 aaaactgatt taggatttat tcctatcagg aaagaagatg attattttac acttgaaaaa
     1981 taaatctaat gcagattctg ttgtagtggt gataagattc tattacctga aactaccacg
     2041 acttttcatc tttcagtaag ttacaaaatg cttcaatgct gaacttgtta agctcattat
     2101 tcccctcttg aattacgtat aatttttctt tctgagtaag atgccagctt aatgatatcc
     2161 gttcttcttt ttgattcatc tgcaaatcaa caaaccttat ctgacggcca tataatgttt
     2221 taagatattc ggaaaaacta tcattaacat aatcctgcat gactataaaa ttgctgctca
     2281 gatttttaaa tggcgacatt actttttccc acaacgagcg gattgttctc atcttactct
     2341 gctcatattt attgttatca cgtatctgaa caaacactac accagaagta ttttccataa
     2401 tccactgcct gaaagcaatg agataacgaa cagaagtttg tacgccatag ttg
//
LOCUS       DAORQT010000243         2443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8803_length_2443_cov_7.627722, whole genome shotgun sequence.
ACCESSION   DAORQT010000243 DAORQT010000000
VERSION     DAORQT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8803_length_2443_cov_7.627722"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PK134_07560"
     CDS             complement(<1..622)
                     /locus_tag="PK134_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38742.1"
                     /translation="MSELLRIFRKINVLAEHHYTNERSSHVEFLSDCHKALAKYFEEY
                     FEDRFIFSALSFSKDDKLHYVDEATDNGYIFNKSYVKNWSELSGLFTKYIKEASDDEP
                     QIISITKILLDKNNTAARKDLFFTCCANCKYENEILVTDSEEDTRVFEHQLGVFNEDK
                     GSYYEANRTKLDKFYEVFSKGSYEQNQNLYDHFSTLLWKQRKLSKSS"
     gene            complement(635..1606)
                     /locus_tag="PK134_07565"
     CDS             complement(635..1606)
                     /locus_tag="PK134_07565"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRB38743.1"
                     /translation="MKAKITGIGHYLPNKIVSNEDLATQYDITGGSERIYQKTGIRQR
                     HYLEIGSTSNLVIKAVKNLIGSNRYRLNDLDCLILGTTTPDYFFPATAIAVLNELNVK
                     NAFGFDVSAACSGFMYGLHLGRLMIESGEAKKIIVCGGDKMSSTLNDHDYKTAVLFGD
                     GAGAIMLEAAEEKEENHLYKSICSMKKDGLSEVYLKTGLNTKQKTIDTIHMEGRRVYE
                     SGVTQMTKSIADYLKSMNLTLDDIDYIVPHQSNLRMIEAVADKLGLSMDKLLTNIETM
                     GNTGAATIPICLSQCYKNTQIKKGDRLLLVGFGAGYSSAVCYLKWSI"
     gene            complement(1563..>2443)
                     /locus_tag="PK134_07570"
     CDS             complement(1563..>2443)
                     /locus_tag="PK134_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB38744.1"
                     /translation="TMKAKHRGYGSMIVVSFGVLGCVLAALLFEFAEVHWQTLFLIGG
                     IAGILLLIFRYSVNETSHFLDKENQQSERGSLTMVLKNKRLLKIYFNSIMLGFPIYFF
                     VSIPIKFAADYGKEIGLTIKGTIPIIIFYVALSISDIIANYLSQVFKSRKKVLYFYVG
                     LCAIPVFLLHFYPPTTPEQYFYIFSPLMGIASGYWALLITYTSEQVGTNIRSTYATAV
                     PNVVRSLFIPVQLLLTALQPSFGTTTSVFYIGILAIILAFWGLSSLKETWGRELNFIK
                     IKNESKNNRHRTLFTE"
BASE COUNT          766 a          489 c          376 g          812 t
ORIGIN      
        1 tcgaactctt agaaagcttg cgctgtttcc acagcaaagt ggaaaaatgg tcatatagat
       61 tctgattctg ttcatagctt cctttactaa acacttcgta aaatttatct aatttcgttc
      121 tgttagcttc gtaatagctt cctttatctt cattaaaaac gcccaattga tgttcaaata
      181 cccttgtatc ttcttcgctg tcggtaacaa gaatttcatt ttcatattta caattcgcac
      241 agcaagtaaa aaacaagtct ttgcgagcag cagtattgtt tttatcaagc aatattttag
      301 tgatgcttat tatttggggt tcatcgtctg aagcttcttt tatgtactta gtaaacaatc
      361 ccgacaactc actccaattc tttacatagc tcttattaaa gatataaccg ttatcagttg
      421 cttcatcaac ataatgtagt ttatcatctt ttgaaaaaga aagagctgaa aaaataaatc
      481 tgtcctcaaa atattcttca aagtatttag ccaatgcttt atggcaatcc gataaaaact
      541 caacatgact acttcgctca tttgtgtaat gatgttcagc aagcacattt attttcctga
      601 atattctaag tagttcactc ataagcaata gtttttaaat actccattta agataacata
      661 cagccgatga ataacctgct ccaaaaccta ctaacaaaag cctgtcgccc ttttttatct
      721 gcgtattttt atagcattgc gaaaggcaaa tagggattgt tgcagcacct gtattgccca
      781 tagtttcaat attggtaagc aacttatcca tagacaaacc gagtttgtcg gcaacggctt
      841 ctatcatccg taagtttgac tgatgaggaa caatgtaatc aatatcatca agcgtaagat
      901 tcattgattt caaataatct gcaatagatt tagtcatttg tgtaacacct gattcatata
      961 cccgtcttcc ttccatgtga attgtgtcaa tcgtcttctg cttagtattc aaccctgttt
     1021 tcaaatacac ttccgaaagt ccgtcttttt tcatcgaaca aatgcttttg tacaagtgat
     1081 tttcttcttt ttcttctgca gcctctaaca ttatcgcacc tgctccatcg ccaaaaagaa
     1141 cggcagtttt atagtcatgg tcgttgagag ttgaactcat cttatcacca ccgcaaacaa
     1201 tgattttttt tgcttcgccc gattctatca ttaacctgcc caaatgcagt ccatacataa
     1261 agccggaaca tgctgccgac acatcaaagc caaaagcatt ttttacattc agttcattta
     1321 acacggctat tgctgttgcc ggaaaaaaat agtcaggcgt tgttgttcca agaattaaac
     1381 aatccaaatc gtttagccta tatctattac ttcctattaa attttttacg gcttttatca
     1441 ctaagtttga tgtgcttcct atttctaaat aatgcctttg gcgaatgcct gttttctgat
     1501 aaattctttc agagccgcca gttatatcgt actgtgtagc caaatcttca ttgctgacta
     1561 ttttattcgg taaataatgt cctatgcctg ttatttttgc tttcattttt tattttaata
     1621 aagtttaatt ctctacccca agtttctttt aaagaactaa gtccccaaaa ggctaatata
     1681 attgccaata tcccaatata aaaaacagag gttgttgttc caaatgaggg ttgtaatgcc
     1741 gttagtagaa gttggaccgg tataaacaaa gaccgaacaa catttggcac tgccgtagca
     1801 taagtggaac ggatatttgt gccaacctgt tcgcttgtat aagtaattaa taaggcccaa
     1861 taacccgatg caattcccat tagaggagaa aagatgtaaa aatattgctc gggtgttgta
     1921 ggtgggtaaa agtgcaacaa aaacacagga atggcgcaca gtccaacata aaagtacaac
     1981 actttttttc tgcttttgaa tacttgactt agatagttag ctataatgtc gctgatagaa
     2041 agggcaacgt aaaaaataat tataggaatt gttcctttaa tagtaagccc tatttcctta
     2101 ccataatcgg cagcaaattt tatcggaata ctcacaaaaa agtaaatagg aaatccaagc
     2161 attattgaat taaaatatat ttttagcaac cttttatttt ttagcaccat tgttaaactt
     2221 ccccgttcac tttgctgatt ttctttgtct aagaagtgag aagtttcatt gacactatat
     2281 cggaatataa gcaacaatat acctgcaatt ccacctataa ggaaaagagt ttgccagtgg
     2341 acttctgcaa actcaaacaa caaagcagcc aagacacaac ctaaaactcc aaatgaaacc
     2401 actatcatag agccataacc tctatgtttg gctttcatgg ttt
//
LOCUS       DAORQT010000244         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8834_length_2436_cov_6.453591, whole genome shotgun sequence.
ACCESSION   DAORQT010000244 DAORQT010000000
VERSION     DAORQT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8834_length_2436_cov_6.453591"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PK134_07575"
     CDS             <1..502
                     /locus_tag="PK134_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38745.1"
                     /translation="LKKESEGLYRNKEAMNFSKAKNIGILFDATVEEDFDLVKKYIKF
                     LKDSKKRVRSFGYYNTKEIPAMQYAKLEVDFFTKKQVNWHLKPSDPLITNFTEEEFDI
                     LINFTVHQCLPLVYIAALSKAKFKIGKHFPKYEMYYDLLLEVEEGKSLKFFMRNVDVY
                     LNMINA"
     gene            565..1461
                     /gene="dapA"
                     /locus_tag="PK134_07580"
     CDS             565..1461
                     /gene="dapA"
                     /locus_tag="PK134_07580"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRB38746.1"
                     /translation="MNSKFKGTGVAIVTPFKKDFSVDEPALKKLVNHLIDGKVEYLVI
                     LGTTGESVTLSKKEKAQVTACVVKETAGRIPLVLGLGGNHTAELLEQLSSTDFTGIDA
                     LLSVSPAYNKPNQEGIYQHFKAIAAASPLPIILYNVPSRTGSNMSAETTLRLAAEFPK
                     FIGIKEASGNLEQCMRIAKSKPKDFLLISGDDNLTIPMISIGAKGVISVIANSFPFET
                     SEAVRHALKDEFDKACGHYFSLMEITDLLFADGSPGGVKAVLKMMDICTDTVRLPLMN
                     VSNSVEEKLKALVDKKRAVIAG"
     gene            complement(1448..1915)
                     /locus_tag="PK134_07585"
     CDS             complement(1448..1915)
                     /locus_tag="PK134_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRB38747.1"
                     /translation="MLPPLNLPSFAVKLKNQSQRTQIFDRIRKKFVSLTPEEWVRQHF
                     LNYLIEHKKYPESRIAVESSLIYNELLRRPDIVYFDVFRKAQLIVECKAPEIKITQST
                     FDQIARYNIALKVRYLVITNGIQHFCCSMDYEKESYAFQKEIPDFSDFETSLQ"
     gene            1987..>2436
                     /locus_tag="PK134_07590"
     CDS             1987..>2436
                     /locus_tag="PK134_07590"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682389.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRB38748.1"
                     /translation="MKSKKDIVDNWLPRYTGTKLEDFGEYILLTNFNNYVKMFAEWHN
                     VEINGADKPMPNATFENITIINFGMGSANAATVMDLLSAISPKAALFLGKCGGLKKKN
                     ELGDLILPIAAIRGEGTSNDYFPSEVPALPAFMLQKAVSTTIRNHNKD"
BASE COUNT          799 a          396 c          496 g          745 t
ORIGIN      
        1 tcttaaaaaa gaatcggaag gtctatacag aaacaaagaa gccatgaatt tttccaaagc
       61 aaaaaatata ggaattctct ttgatgctac agtggaagaa gattttgatc tggtaaagaa
      121 atacattaaa tttttaaaag acagtaaaaa acgggtacgc tcctttgggt attacaatac
      181 caaagaaatt cccgccatgc agtatgccaa gttagaagtt gattttttta ccaagaagca
      241 agtcaattgg cacttgaaac caagcgatcc cttaattact aattttacgg aagaggagtt
      301 tgatatttta attaatttca ctgttcatca atgtttgcct ctggtttata ttgctgcatt
      361 gagtaaagcc aaatttaaaa taggcaagca ttttcccaag tatgaaatgt attacgattt
      421 gttgctagag gtggaagaag gtaaaagtct caaatttttt atgagaaatg tggatgtgta
      481 tttgaatatg ataaacgcct gaaaaaagga attacactca aggcaatact gtgtgttgct
      541 ataaactgaa aattaaaaga aaacatgaat tcaaaattta aaggtaccgg agtagctatt
      601 gtgactcctt ttaaaaagga tttttctgtg gatgaaccgg cattaaaaaa gctggtgaat
      661 cacttgattg atggcaaagt ggaatacttg gtcattttgg gtactacagg agaatctgtc
      721 accttaagca aaaaggaaaa ggcgcaagta acggcttgtg ttgtgaaaga aactgctgga
      781 cgaattccat tggtattagg actaggcggt aaccatactg ctgaactatt ggagcaactt
      841 tcgtcaactg attttaccgg aattgatgca ctgctttctg tttctcctgc atacaataaa
      901 cccaatcagg aaggaattta tcagcatttt aaagcaattg cagctgcttc tcctttgcca
      961 attatacttt acaatgttcc tagccgcacc ggttctaaca tgagtgcaga aactaccctg
     1021 agattggcag cagagtttcc caaatttatt ggcatcaagg aagccagtgg aaatttggag
     1081 caatgcatgc gtatagcaaa atcaaaaccc aaagattttt tgttgatatc cggggatgat
     1141 aatttaacga ttccaatgat atcgattggt gcaaaagggg ttatttctgt tatcgccaat
     1201 tcctttccgt ttgaaacgtc agaggctgtt cgacatgctt taaaagatga gtttgacaaa
     1261 gcctgtggcc attacttttc tttaatggaa attactgatt tgctttttgc agatggcagt
     1321 ccgggtggag taaaagcagt tttaaaaatg atggacattt gcacggatac ggttcgactt
     1381 ccattaatga atgtgagcaa ctcagtagaa gaaaaattga aagcactggt ggataagaaa
     1441 agagctgtta ttgcaggcta gtttcgaaat ctgaaaaatc aggaatttct ttttggaaag
     1501 cataactttc cttttcataa tccatgctgc agcaaaaatg ctgtattccg tttgttatca
     1561 ctaaatagcg cacctttaaa gcgatgttat aacgcgctat ttggtcaaaa gttgattgcg
     1621 tgatttttat ttcaggtgct ttgcattcta caatcaattg tgctttccta aatacatcaa
     1681 aataaacgat atccggcctt cttaacaatt cattgtaaat aagcgaggac tctacagcta
     1741 tgcgtgattc cggatatttt ttgtgttcta ttaaataatt tagaaagtgt tgccttaccc
     1801 attcttcggg agtaagacta acaaatttct ttctgattct gtcaaaaatt tgcgttcttt
     1861 gagattggtt ttttagcttt acagcgaagg aaggaaggtt gagcggagga agcataaaag
     1921 ttaaaatttc gaaacaaata taaactttgc ttcgcgtata gaagtaatgt gtagaaatat
     1981 gaaagcatga aaagcaaaaa agacattgta gacaattggt tgcctcgtta cacaggaact
     2041 aaattagaag attttggtga gtatattttg ttgaccaatt tcaacaacta tgtaaagatg
     2101 tttgcagaat ggcacaatgt tgaaattaat ggggccgata aacccatgcc caatgcaact
     2161 tttgaaaaca taaccattat taattttgga atgggcagtg ccaacgcggc tacggtaatg
     2221 gatttgctca gtgccatttc tcccaaagca gcattgtttt taggaaaatg cggaggatta
     2281 aaaaagaaaa atgaattggg tgacttgata ttgccgattg ctgcaattcg aggtgaagga
     2341 acatcaaatg attattttcc atctgaagta ccggccttac ctgcttttat gcttcagaag
     2401 gctgtgtcta ccaccattcg caaccacaat aaagat
//
LOCUS       DAORQT010000245         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_8875_length_2429_cov_6.930918, whole genome shotgun sequence.
ACCESSION   DAORQT010000245 DAORQT010000000
VERSION     DAORQT010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8875_length_2429_cov_6.930918"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PK134_07595"
     CDS             <1..608
                     /locus_tag="PK134_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB38749.1"
                     /translation="LETRRLQFVSTLGLQGVSETAAQKEVSAAVDMLVYYAGWCDKYQ
                     QVFSSVNPVESSHFNFSFPEPTGVVTAIASPEQGLLNLVSLMAPSLAGGNSIIILAAE
                     KFPLTAIDFAEVLNASDVPGGVVNILTGYSKELHTHFSSHMDVNAIVYAGDNSAELKT
                     IQTNAAGNVKRVVVVQNIAEESPYRIMELQEVKTTWHPVGI"
     gene            773..1996
                     /locus_tag="PK134_07600"
     CDS             773..1996
                     /locus_tag="PK134_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38750.1"
                     /translation="MKTFFNLIFFLFVGMNLKAQTYGCTDSLANNYNANATANDGSCI
                     YNSANVVPLSSNLLPIALDETSGLTFWNNMLWSLNDDADNNLYAIDTGNFNNYTAYPL
                     NEPNIDWEEISQDDDYFYIGDFGNNVSGNRTDLKILKIEKNSLLQNNPNVDTINFSYS
                     TQTNFNPTAINSTDFDCEAFVITADSIYLFTKEWISNHCSVYSLPNKSGSYSANFISS
                     YNVNGLVTGATQIQNSRAVVLSGYSTVMQPFVLLLYDYQGFNFFSGNKRIINVNLPFH
                     QTEGIATVNGLKYYLSNEKLVQTPINIQAKIHEIDLTPYLSHYLNSVSGSFDISENPY
                     AINIYPNPTTDFINVKWNTGVKVARIKIFNISGKEVMNQIVTEKYFQIDIRSFEKGIY
                     FLSVNDSVFRKVVKM"
     gene            2128..>2429
                     /locus_tag="PK134_07605"
     CDS             2128..>2429
                     /locus_tag="PK134_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38751.1"
                     /translation="MKVISADDKSATSLSDSAQLRYITFILLYFSQGIPEGLTLFAIP
                     AWLAFNGKSAVEIAAYSATIMIPFSLKILLAPIIEKYTYLPMGRRRPWLLFGQFGI"
BASE COUNT          790 a          378 c          454 g          807 t
ORIGIN      
        1 ctctggaaac acgaagacta caatttgtgt caaccttagg tctgcaaggt gtgagtgaaa
       61 cggcagcaca aaaggaagtg agtgcagcag tagatatgct tgtttattat gcaggatggt
      121 gcgataaata tcagcaggtt ttttcaagtg tgaatcccgt agaatcatca cattttaatt
      181 tttcttttcc tgagccaaca ggagttgtta cagcaattgc atcgccagaa caaggtttat
      241 taaatctggt ttctcttatg gcaccttcac ttgcaggagg taattcaata attattctgg
      301 cagcagaaaa atttcctctc acagcaattg attttgcaga ggtgcttaat gcaagcgatg
      361 tgcccggtgg tgttgtaaat atacttacag gttatagtaa agaattgcat acgcactttt
      421 cgtcacacat ggatgttaat gccattgttt atgcaggtga taacagtgct gaattgaaga
      481 ccatacaaac caatgcagca ggtaatgtta aacgcgtagt agtagttcag aatattgctg
      541 aagaaagtcc atatcgcatc atggaacttc aggaagtaaa aaccacatgg catcctgtgg
      601 gtatttgagc ttgggtcaat atatattttt gtattcatga atgcaatatc tttgacctga
      661 acgaaagcca aggctatcgt ttagctgtga aatgatcaaa tattaattgg caaaattgaa
      721 gtcaagtgct gtcttctgac agaagtgatt tatttgaatt gtaaacacat ttatgaaaac
      781 attttttaat ttgatttttt tcttgtttgt aggaatgaat ctgaaagcac aaacttatgg
      841 ttgtacagat tcgttagcca ataattataa tgccaatgca actgcaaatg atggtagttg
      901 tatttataac tcagccaatg ttgttccatt gagtagtaat ttattgccaa ttgcattaga
      961 cgaaacatca ggattaactt tttggaataa tatgttgtgg tcgctgaatg atgatgcaga
     1021 taataattta tatgcaattg acaccggaaa ttttaataac tatacagctt atcctttaaa
     1081 cgagcctaat attgactggg aggaaatttc gcaggatgat gactattttt atataggtga
     1141 ttttggaaat aatgtcagcg gaaacagaac cgatttgaaa atattgaaaa tcgaaaaaaa
     1201 ctctttactg cagaacaatc ctaatgttga tacaataaac ttttcttatt caacgcaaac
     1261 aaattttaat cctactgcca taaacagcac cgattttgat tgtgaagctt ttgtaataac
     1321 agccgacagt atttatttgt ttacaaaaga gtggataagc aatcattgct ctgtttattc
     1381 tcttccaaac aagtcgggta gttatagtgc aaattttatt tcatcctata atgtgaacgg
     1441 actcgttacc ggtgctaccc aaattcaaaa tagtagggca gttgtacttt caggctattc
     1501 aacagtgatg caaccttttg ttttactttt atatgattat cagggattca atttttttag
     1561 tggtaataaa agaattatca atgttaatct gccttttcat cagactgaag gcatagcaac
     1621 tgttaatgga ttaaaatatt atttgtccaa tgaaaaactt gtacaaacac caataaacat
     1681 tcaggcaaag attcatgaaa tagatttaac gccatattta agtcactatt tgaattctgt
     1741 ttccggaagt tttgatatat ctgaaaatcc ttatgcaata aatatttacc caaatcctac
     1801 aacagatttt attaatgtta aatggaatac aggagtgaaa gttgcacgaa ttaaaatttt
     1861 taacatcagc ggaaaagagg tgatgaacca aatagtaaca gaaaagtatt ttcagattga
     1921 catcagaagt tttgaaaagg gaatttattt tctgagtgtt aatgatagcg tattccggaa
     1981 agtagtcaaa atgtgactgt attgaaaaac agattgtagt tcgttgactt tatattactg
     2041 gtttgtgtat tttagcttgc agttaaatca atagacatat tatctttgtt ttaactttac
     2101 aacacctaac aatctaaatt aaattttatg aaagtcattt ctgcagatga caagagtgca
     2161 acatcgcttt ccgatagtgc gcaacttcga tatattacct tcattttatt gtatttctca
     2221 caaggcattc cagaggggct gacacttttt gctatccctg catggctggc tttcaatggt
     2281 aagagtgctg ttgaaattgc agcatatagt gcaactatta tgattccgtt tagtttaaaa
     2341 atattgctgg ctccaatcat agaaaaatac acctatctgc ctatgggacg cagaagacct
     2401 tggttgttgt ttggtcagtt tggaattat
//
LOCUS       DAORQT010000246         2394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9080_length_2394_cov_5.091920, whole genome shotgun sequence.
ACCESSION   DAORQT010000246 DAORQT010000000
VERSION     DAORQT010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9080_length_2394_cov_5.091920"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PK134_07610"
     CDS             <1..772
                     /locus_tag="PK134_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB38752.1"
                     /translation="HSTEKFEPGLGALKFSQLRWGMLAIFVYVGVEVTIQSNMGALLA
                     LPQFGGLTTAQISPYISLYWGSLMIGRWTGAISAFNFKPSTKKLLMIVLPIVAYIIVL
                     LANYLRGNEITNLLPYSLCVAALIAGSFLAQEKPARMLFIFSAGGILAMLIGLFTEGQ
                     VAMFAFISGGLCCSIGWPCIFALSITGLGKFTGQGSAFLIMMILGGALIPPLQGYLAD
                     VTDIHSSYWITVFCFAYLAWFAIKVKVILQKQGFHTNQ"
     gene            841..>2394
                     /locus_tag="PK134_07615"
     CDS             841..>2394
                     /locus_tag="PK134_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRB38753.1"
                     /translation="MQPQKEFKPYISATDSMAEFTLKSILLGSMFGIIFGASTVYLAL
                     KAGLTVSASIPIAVLTISIGRKFFKTTILENNIIQTTGSAGESIASGVVFTLPAFLFL
                     SVNESGISNGIQYFEYWTIFTLAVLGGMLGTLMMIPLRRSLIVKEHETLPYPEGTACA
                     QVLIAGEKGGDFARTAYLGLGFAMLYAIFQKVFHVIAEVPAFVTKQTNKYLPSATVSG
                     EITPEYLGVGYIIGPKIGGVLVAGGVLAWLGLIPLLATLVPTDMIAAQLVKLGYLKDV
                     LTASGPGGWNPETHTFTDMADAIYRAYVRQIGAGAVAAGGFITLLKTIPTIISSFKES
                     VSSLKSGDKEQKVSRTENDLSFKTVIVGSVVLIILMALLPQVPGESFLNKLLIGLLIV
                     VFGFFFVTVSSRIVGIIGSSNNPISGMTIATIMGTALVFIAIGWTGKVFEPMALVVGG
                     LVCIAAANAGATSQDLKTGYIVGATPRYQQMALFIGAIVSSVVIGFTVKYLDTPTADL
                     TAQGIHHAIG"
BASE COUNT          651 a          432 c          509 g          802 t
ORIGIN      
        1 acacagcaca gaaaagtttg aaccaggttt aggtgcttta aaattttcgc aactgcgatg
       61 gggcatgctt gctatctttg tttatgttgg cgtagaggta accattcaaa gcaacatggg
      121 tgcccttctg gcattgccgc agttcggagg actcacaaca gctcaaatat ctccttacat
      181 ttctctttat tggggaagtt taatgattgg tcgttggaca ggtgccatca gtgcttttaa
      241 ttttaagcct tcaaccaaaa aactgcttat gattgtgttg ccaatagttg catatatcat
      301 tgttttatta gccaactatc ttcgtggtaa tgaaattaca aatctgttgc catacagtct
      361 ttgtgttgca gcactcatag ccggaagttt tttagctcag gaaaagcctg cgcgcatgtt
      421 gtttattttt tctgcaggag gtattttggc aatgttaata ggtcttttta ccgaagggca
      481 ggtagccatg tttgccttca tcagtggtgg cttgtgttgc agtatcggat ggccttgtat
      541 ttttgcttta agtataacag gattaggaaa gtttaccgga caaggctcgg cttttctgat
      601 tatgatgata cttggaggag cattaattcc tccattgcag ggttatcttg ccgatgtaac
      661 agacattcat tccagctatt ggataacggt cttttgcttt gcgtacttag cctggtttgc
      721 cattaaagtt aaagtaattt tgcaaaaaca aggttttcat accaatcagt aaaagcgtta
      781 acattttttt gttttggcta attaaaatat cctttacatt agccatctca ctttttattt
      841 atgcaaccac aaaaagaatt taagccatac atctcagcaa cagattcaat ggcagagttt
      901 acactcaaat caattctgct tggctccatg tttggaatca tcttcggggc atcaacagtt
      961 tatctggcat taaaagcagg gttgactgtc tctgcttcga ttcccattgc tgttcttaca
     1021 ataagtattg gccgcaagtt ttttaaaact actattcttg agaataatat tattcagaca
     1081 acaggctctg caggagagtc tattgcctcc ggtgtggttt ttactttgcc tgcattttta
     1141 tttttatctg taaatgaaag tggcatcagt aacgggattc agtattttga atattggaca
     1201 atctttacat tagctgttct gggaggaatg ttgggtacat taatgatgat tcctctacgc
     1261 agaagtctta tcgttaaaga gcatgaaaca ttaccttatc ccgaaggaac tgcctgtgct
     1321 caggttctta tagcaggcga aaaaggtggt gattttgcac gcacagccta tcttggttta
     1381 ggctttgcca tgctttatgc catttttcaa aaagtatttc atgtcattgc agaagtacct
     1441 gcatttgtaa ctaaacaaac caacaaatat ctgccatcgg ctactgttag tggagaaatt
     1501 actcccgaat atcttggtgt tggatatatt attggtccaa aaataggagg ggtgcttgtt
     1561 gccggtggtg tgctagcatg gcttggatta attcctttgt tagcaacatt agtgcccact
     1621 gatatgattg cagcacaact tgtgaaatta ggctacctaa aagatgtttt aactgccagt
     1681 ggacccggag gatggaatcc tgagacacat acatttactg atatggccga tgcaatttat
     1741 cgtgcttatg taagacaaat tggtgcaggt gctgtggcag caggaggatt tatcacacta
     1801 ttgaaaacaa taccaacaat tatttcttct tttaaagaaa gcgtaagctc gctgaaaagt
     1861 ggagataaag agcagaaagt ttcacgtact gaaaatgatt tatcattcaa aacagtaatt
     1921 gtaggttctg tagttttaat tattctgatg gcattattac ctcaggtgcc cggtgaatcg
     1981 ttcctgaata aattattgat tggattacta atcgtagtgt ttggattttt ctttgtaacg
     2041 gtttcaagtc gcattgttgg tatcatcggt agcagtaata acccaatttc agggatgacc
     2101 attgcaacca tcatgggaac agcattagtg tttatcgcaa taggatggac tggaaaagtt
     2161 tttgaaccta tggcacttgt tgtaggtggt ttggtttgta ttgcagctgc aaatgccggt
     2221 gccacttcac aagatttaaa aacaggttat attgttggag caacaccacg ttatcagcaa
     2281 atggctttat tcattggtgc tattgtttcg tcagtagtta taggtttcac tgtaaaatat
     2341 cttgacacgc ctactgccga cctgactgca caaggtattc atcatgccat tggt
//
LOCUS       DAORQT010000247         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9110_length_2390_cov_5.800000, whole genome shotgun sequence.
ACCESSION   DAORQT010000247 DAORQT010000000
VERSION     DAORQT010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9110_length_2390_cov_5.800000"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PK134_07620"
     CDS             <1..642
                     /locus_tag="PK134_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38754.1"
                     /translation="NQHTILYKGELKPNSYVCLSIFKRDALINLDSFYIQILIAADGE
                     YKRPFTKDIAVELPELNQTKNTETGILSSSKKVSLVNLNVADEIQIKDLLEKFDSSQP
                     AKTSDIKKNKKQQPISDSDISKEIDLHINKLTADFQNMTNSEMLALQINTFRKEMDNA
                     IIKDYKQLIFIHGIGDGILRNAIMNETKNYSDIKVFMANQARYGTGALEIRFL"
     gene            complement(637..>2390)
                     /locus_tag="PK134_07625"
     CDS             complement(637..>2390)
                     /locus_tag="PK134_07625"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38755.1"
                     /translation="ISMMHYRFSVSNAASHFVDVELTIQTDGNETLLKLPVWRPGRYE
                     EGNFAKNIRNFRVQDINGKEVKWKKVSKSSWLLKTNKNDTIKINYQYYCNQLDAGACF
                     VNDEQIYINPIHCCFYTDANRGNACTVEIVSNFSRVATSLTQTEKNVFSAKNFDELVD
                     SPFIASDDLMHYGYTVSDCSFHIWIQGNFKPDVEKLIEDFKSFTQIQLNVMKSIPVKD
                     YHFLIQAVPHTFYHGVEHLASTVLVIGPSAKLNDRELYNELLGVASHELFHVWNIKTI
                     RPVEMQPYNYDSPNYAETGFVYEGATTYYGDLFLARCGYFNEEEYFNELNIRINKHLS
                     NEGRFNYSIAQSSFDTWLDGYVPGIPGRKTSIYDEGSLVSLMLDFLIRKYSDGKNSLD
                     DVFIELYNAFGKKNIGYTSDDYLRIASEKAGRSLETFFNDIVYKACSYQSLLEEVMLF
                     AGLELEENPSKKNAERYFGFKTNDNAAMPEVISLPIGSPAFENGLAIGDLIIGVDGQK
                     ADKNYDMQINPKENHKTGFLISRGNRIKTIEMMPASKKYFSTWKVKHISKKNAEQEQF
                     FNQWTNNLRAHHSQNSL"
BASE COUNT          798 a          447 c          369 g          776 t
ORIGIN      
        1 aaccaacaca caattttata caagggtgaa ttaaaaccca atagttatgt atgcctttct
       61 atatttaaac gagacgcatt aattaactta gattcgtttt acatacaaat attaattgct
      121 gccgatggcg aatataaaag accttttact aaagatattg ctgttgaatt gcctgagctg
      181 aatcaaacta aaaatacaga aacaggaatt ctttcttctt ctaaaaaagt aagccttgtc
      241 aatctgaatg ttgcagatga aattcagata aaagacttat tagaaaaatt tgacagcagt
      301 cagccagcaa aaacttcaga catcaaaaag aataaaaaac aacaaccaat aagcgattct
      361 gacatcagca aagaaattga tttgcatata aataaactta ctgctgattt tcagaatatg
      421 accaacagcg aaatgcttgc gttacaaata aatactttca gaaaagaaat ggacaatgca
      481 attataaaag attacaagca gctaattttc attcatggca ttggtgatgg tatattgcga
      541 aatgcaataa tgaatgaaac taaaaattat tcagacataa aagtgtttat ggctaatcag
      601 gcaagatatg gaacaggtgc tttggaaata cgctttttat aaactattct gcgaatgatg
      661 tgcacgaagg ttgttcgtcc actgattaaa aaactgttct tgttctgcgt tctttttgct
      721 gatgtgtttt accttccagg tcgaaaaata tttttttgat gcaggcatca tttcaatagt
      781 ctttattctg ttacctcttg aaatcaaaaa acctgttttg tggttttcct taggattaat
      841 ctgcatgtca taatttttat ctgccttttg cccatctacg ccaataatca aatcacctat
      901 agcaagacca ttctcaaatg ccggtgaacc tattggcaaa ctaattactt ctggcattgc
      961 tgcattatca ttggtcttaa aaccaaaata tctttctgca ttctttttag aaggattctc
     1021 ttctagttcc aatcctgcaa acaacatcac ttcttcaagc aacgattgat agctgcaagc
     1081 cttgtaaaca atgtcgttaa agaaagtttc taaacttcta cctgcctttt cagaagcaat
     1141 tcttaaataa tcatctgatg tatatccaat attcttttta ccaaacgcat tatacaattc
     1201 tataaaaaca tcatctaatg aattttttcc gtcagaatat ttacgaatca aaaaatcaag
     1261 catcagcgac actaatgagc cttcatcata aattgaagtt tttcgtccgg gaattcccgg
     1321 cacatatcca tcaagccagg tgtcaaaaga tgattgtgca attgaataat taaaccggcc
     1381 ttcattgcta agatgtttgt tgatacggat gttcaactca ttaaagtatt cttcttcgtt
     1441 gaaataacca cagcgtgcca aaaacaaatc gccataatat gttgtagctc cttcatagac
     1501 aaatcctgtt tctgcataat ttgggctatc ataattgtaa ggttgcattt ctacaggtct
     1561 tatggtttta atattccaca catgaaacaa ttcatgtgat gcaactccca gtaactcatt
     1621 atacaattct ctgtcattaa gttttgccga agggccaatc actaaaacag tagatgccaa
     1681 atgttcaaca ccatgataaa atgtatgtgg cactgcctga attaaaaagt gataatcttt
     1741 tacaggaatt gatttcatca cattcaattg tatctgcgta aaagatttaa aatcttcaat
     1801 caacttttct acatcgggtt tgaaatttcc ctgaatccaa atatgaaaac tgcaatcaga
     1861 caccgtataa ccatagtgca tcaaatcatc agaagctata aacgggctgt caacaagttc
     1921 atcaaaattc ttagcagaaa aaacattttt ttctgtttgt gtgagtgatg ttgctactct
     1981 gctgaagtta ctaactatct caactgtgca ggcattgccc ctgttggcat cagtataaaa
     2041 acaacaatgt atgggattta tgtaaatctg ttcgtcattg acaaagcagg caccggcatc
     2101 aagctgattg caatagtact gatagttgat ttttattgta tcatttttat ttgttttcag
     2161 cagccatgag ctctttgaaa cttttttcca tttcacttcc tttccattaa tatcctgcac
     2221 tctaaaattg cggatatttt ttgcaaagtt tccttcttcg tacctgcccg gacgccagac
     2281 aggaagtttt aataaagttt cattaccgtc agtttgtatg gttagttcaa catcaacaaa
     2341 gtgtgaagct gcattggata ctgaaaaacg ataatgcatc atgctaataa
//
LOCUS       DAORQT010000248         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9233_length_2369_cov_3.136560, whole genome shotgun sequence.
ACCESSION   DAORQT010000248 DAORQT010000000
VERSION     DAORQT010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9233_length_2369_cov_3.136560"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..596
                     /locus_tag="PK134_07630"
     CDS             57..596
                     /locus_tag="PK134_07630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB38756.1"
                     /translation="MNVDLISRGISEALKGEKTALTMEQVQACIGAYMGEKQKVKDQE
                     AKAKAGVNLEKGKKFLEENGKRKGVVTLPSGLQYEIIKEGTGPKPAATDKVTTHYHGT
                     TIDGKVFDSSVERGQPAQFGVNQVIPGWTEALQLMPVGSKWKLFIPANLAYGESGAGG
                     AIGPNETLVFEVELISIDK"
     gene            complement(645..>2369)
                     /locus_tag="PK134_07635"
     CDS             complement(645..>2369)
                     /locus_tag="PK134_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38757.1"
                     /translation="QGLKAIPQIFSTSYRSGYYLGTDELYRPIPKSIFAISWNWFGNN
                     PLPLHLLNIVLFGITGIVLFNFCYLLFPNLVFSFIASMLFMFHPVHTEVVANIKSVDE
                     ILSFLLCISACIFLARYVQTKRISELVLTSVYFFAALLSKESSITFLAVFPLLILLKS
                     DKKETSYNWSVELKPMLTLTAFALLFLMIRHSVLSKIASGDPSIADNLLMAAKSSDQK
                     FATAVMILGMYLKLLFIPHPLVFDYSYNQIPLVGMGNIWFLLSLLIHVGLLFWAILLL
                     KKNKLLSFIILFYFISMSIYSNIFYVIGSSFGERFLYTASFGFCMAVAYAFSLLSKNQ
                     NVETVSSFFKNNLTVFLIFLPVAFAFGYKTITRTTVWKSNYTLYANDVNLSPNSTSTH
                     YYYGNLLAKDDFQKDKTDQEKDSIQKLALSELEKSVAIFPFGDAYNQMGIIYDKRKEF
                     KKSFESYQQAMKAAPNDPMIQNNLGSLYFNMGQIPEAKACYEKAVQLNPSYSEALMNL
                     GSACGMMKQYDDAVTNYLKAIKFDPNLAQAYYYLGLTYRFKGDQQNSEFYYNQAAKMD
                     PEKYGKKK"
BASE COUNT          854 a          385 c          448 g          682 t
ORIGIN      
        1 gctatggtat aggagtttct attggcgaaa acatgaaaaa agacggacta accgacatga
       61 atgttgactt gatttcaaga ggtatttccg aagctttgaa aggtgaaaaa accgccttaa
      121 ccatggagca agttcaggct tgcattggag cgtatatggg cgaaaaacaa aaagtaaaag
      181 atcaggaagc gaaagcaaaa gccggtgtta acttagaaaa aggtaaaaaa ttcttagaag
      241 agaatggtaa acgcaaaggt gtggtaacat tgccaagcgg ccttcagtat gaaattatta
      301 aagaaggtac cggtccaaaa ccagcagcta cagataaagt aactacacat tatcatggaa
      361 caaccattga tggtaaagta tttgatagtt ctgttgaaag aggtcaacct gcgcagtttg
      421 gcgttaatca ggtaataccg ggatggacag aagcattgca attgatgcct gttggttcta
      481 aatggaagtt gtttattcct gcaaacttag cttatggcga aagtggagca ggtggagcca
      541 ttggtccaaa tgaaacattg gtttttgaag ttgaattaat ttctattgat aagtaatcgc
      601 aatataataa tcaaacttaa agctgtctgc aaaggcagct ttttttattt cttcttaccg
      661 tatttttcag gatccatttt tgctgcctga ttataataga attcagaatt ttgctgatcg
      721 cctttaaaac gataggttaa gccgagataa taatatgcct gcgccagatt aggatcaaat
      781 ttaattgctt ttaaatagtt tgttacagca tcatcatatt gtttcatcat gccgcaggcg
      841 ctgcctaaat tcatcaacgc ttcggagtat gaaggattca attgaacagc tttttcataa
      901 catgcttttg cctcaggtat ctgccccata ttgaaataca atgagcctaa attgttttga
      961 atcatcgggt cgttaggtgc agccttcatt gcttgttgat agctctcgaa tgatttctta
     1021 aattcttttc ttttatcata aataattccc atttgattat aggcatcgcc aaacgggaaa
     1081 atagcaacag atttttctag ttcactcaat gcaagttttt gaatagagtc tttttcctga
     1141 tctgttttgt ctttttgaaa atcgtctttt gccaaaagat tgccataata atagtgcgtg
     1201 ctcgtactgt tgggagaaag gttaacatca ttagcataca aagtgtagtt cgacttccag
     1261 acagtggttc tggtgatggt tttatagcca aatgcaaagg caacaggaag gaatattaga
     1321 aagacagtca ggttgttttt gaaaaaagaa gaaacagttt caacattctg atttttactt
     1381 aataatgaaa atgcataagc tacagccata caaaagccaa aacttgcagt gtataaaaaa
     1441 cgttctccaa acgatgagcc tatcacataa aaaatgttgg aatagatgct catcgaaata
     1501 aaataaaaca gaataataaa tgacaacaat ttatttttct tcaataataa aatagcccaa
     1561 aataataatc cgacatgaat taatagagaa agaagaaacc agatgtttcc cataccaacc
     1621 aatggaattt gattatagga ataatcaaaa acaaggggat gaggaataaa aagtagcttt
     1681 aaatacattc ctaaaatcat aacggcagta gcaaatttct gatcagaaga ttttgcagcc
     1741 atcaacaagt tgtctgcaat agatggatca ccacttgcta tcttactgag aacagaatga
     1801 cgaatcatca gaaataacag tgcaaatgct gttagtgtca acatgggttt taattcaaca
     1861 ctccaattgt atgatgtttc ctttttgtca gacttcaata aaatcagcaa cggaaaaact
     1921 gctaaaaaag taatagatga ttcttttgaa agcagggcag caaaaaaata gactgaagtt
     1981 aaaactaatt cagaaattct ttttgtctga acatatcttg ccagaaaaat acatgcagaa
     2041 atacatagca aaaaactaag tatttcatca acacttttta tgttggcaac aacctctgtg
     2101 tgcacagggt gaaacataaa caacattgat gctatgaaag aaaaaaccag atttggaaaa
     2161 agtaagtaac aaaaattaaa caacacgatg cccgtaattc cgaaaagaac aatattcaac
     2221 aaatggagtg gaagaggatt gttaccaaac caattccatg aaatggcaaa gattgattta
     2281 ggtataggac gatacagttc atcagtacct aaataatatc cacttctgta ggaggtagaa
     2341 aaaatctgag gtatcgcttt taagccttg
//
LOCUS       DAORQT010000249         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9276_length_2362_cov_5.618986, whole genome shotgun sequence.
ACCESSION   DAORQT010000249 DAORQT010000000
VERSION     DAORQT010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9276_length_2362_cov_5.618986"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PK134_07640"
     CDS             <1..140
                     /locus_tag="PK134_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRB38758.1"
                     /translation="LKIGCIEEIAYRMKFIDKEQLRKVAEPLTKSGYGNYLMKIIDQN
                     L"
     gene            155..1099
                     /locus_tag="PK134_07645"
     CDS             155..1099
                     /locus_tag="PK134_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB38759.1"
                     /translation="MKSKILVTGGAGFVGSALAEKLADNADNFVVVVDNLLTGSMRKV
                     PASKHNNVKFIKADVNDFSDISSIFYAYAFDYVFHYAAVVGVKRTLDHPSWVLKDLTG
                     LKNILDLSKNTGVKRVYFSSSSEVYGEPVEYPQNETTTPLNSRLPYAIVKNLGEAYLR
                     AYKQEFDLDFTIFRFFNTYGPKQSTDFVMSKFIEAALSDKDITIYGDGSQTRTFCYVD
                     DNVEACLNAFYTHRFVNDVINVGNDHETSILELAQLVKKILNSKSNIVHLPALKEGDM
                     TRRCPDIGKMKILLNRELTPLETGMERVIEVFRNKKKK"
     gene            1109..2083
                     /locus_tag="PK134_07650"
     CDS             1109..2083
                     /locus_tag="PK134_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRB38760.1"
                     /translation="MESIDFLQREIERELKLLRFGHEPANLYEPMEYIVELGGKRMRP
                     LLVLIGAGLFETNYQKAMKAAIGIELFHNFTLLHDDIMDKATLRRNKPTVHEKWSADI
                     AILSGDALFVRASEYIMQVDDAILRPVMQLFYKTAMQVCEGQQYDMDFGRLREITPEH
                     YIGMITLKTAVLPACCLATGAIIANASEEDKLRLYEFGKNIGIAFQIQDDLLDAFGDD
                     TKFGKRPGGDIIEGKKTFLFSEACRLLNGKEHLDFLTLYNSHNLSNHEKITEVMQVFN
                     NLELHHHTMRTIQSYYQKGLEQLAGLNVQAKNVLELKLFCEKLLIREH"
BASE COUNT          783 a          380 c          474 g          725 t
ORIGIN      
        1 gtttaaaaat tggttgtata gaagaaatag cctaccgtat gaaatttatt gataaagaac
       61 aattgcggaa agtagcagag ccattaacaa aaagcggcta tggaaattat ctgatgaaaa
      121 taatagatca aaatttataa gcgaaacaaa atatatgaaa agtaaaattt tagtaaccgg
      181 tggagcagga tttgtaggaa gtgccctggc agaaaaatta gcggacaatg ctgacaattt
      241 tgttgttgta gttgataatc tgcttacagg ttccatgcga aaggttccgg catcgaaaca
      301 taacaatgtt aaatttatta aagcagatgt aaatgatttc agcgatatat cttccatttt
      361 ttatgcctat gcatttgact atgtttttca ttatgctgct gtagttggtg taaaacgcac
      421 actcgatcat ccatcatggg tattaaaaga tcttacagga ttgaaaaata ttcttgactt
      481 atcaaagaac acaggagtaa aacgtgttta cttcagcagc agcagcgaag tttatggtga
      541 gcctgttgag tatccacaaa atgaaacaac aacaccttta aactcaaggc ttccttatgc
      601 cattgtaaaa aatcttggcg aagcctatct gcgtgcctac aagcaggagt ttgatcttga
      661 ctttaccatt ttccgattct tcaacacata cggacctaag cagagtacag actttgtaat
      721 gtcgaaattt attgaagctg cattgagcga taaagacatt actatctatg gcgatggttc
      781 acaaacacga accttctgtt atgttgatga taatgttgaa gcctgtttaa atgccttcta
      841 cacacatcgt tttgtgaatg acgtcatcaa tgtgggcaat gaccatgaaa cttctattct
      901 ggaattggcg cagttggtaa agaaaattct gaattcaaaa tcgaacattg tgcatttacc
      961 tgctttaaaa gaaggtgata tgacacgcag atgtcctgat attggcaaaa tgaaaatatt
     1021 actcaatcgc gagttaacgc ctcttgaaac aggcatggaa agagtgattg aggttttcag
     1081 aaataaaaag aaaaaataat tttttttgat ggaaagtatt gactttctgc aacgcgaaat
     1141 tgaacgcgaa ttaaagttgc tgcgctttgg tcatgagccg gccaatctat atgagcctat
     1201 ggaatatata gttgagcttg gtggcaaacg catgcggcca ttattggttc ttataggagc
     1261 aggcttgttt gaaacaaatt atcagaaagc aatgaaagct gccataggca ttgagttgtt
     1321 tcacaatttt acactgctgc atgatgacat catggataag gcaactttga gaagaaacaa
     1381 accaacggtg catgaaaaat ggagtgccga tattgcaata ctttccggtg atgcgctttt
     1441 tgtaagagcc agtgaataca ttatgcaggt tgatgatgcc atactaagac cggtgatgca
     1501 gttgttttat aaaacagcta tgcaggtttg cgaaggacaa cagtacgaca tggactttgg
     1561 gcgattgcgc gaaattactc ctgaacatta tattggcatg attactttaa aaacagcggt
     1621 gctacctgca tgttgtctgg caacaggtgc aataattgcc aatgcctcag aagaagacaa
     1681 attacgattg tatgagtttg gaaaaaatat aggtattgca tttcagattc aggatgattt
     1741 gttagatgct tttggtgatg atactaaatt tggtaaacgt cccggtggcg atattattga
     1801 gggtaaaaaa acatttcttt tttcagaagc atgcaggctg ttaaacggca aagagcatct
     1861 tgattttctg acactgtata actcacacaa tttatccaat cacgaaaaaa ttactgaagt
     1921 gatgcaggtg ttcaataact tagaattgca tcatcatacc atgcggacaa ttcaatccta
     1981 ttatcaaaaa gggttggagc aacttgcggg cttaaatgtg caggcaaaaa atgttcttga
     2041 attaaaatta ttttgtgaga agctgttgat aagggagcat tgaaactgtg taataacctt
     2101 ttctggaaat taatttaaac cttacattta cgtttttgtg aatcacaact attttacaca
     2161 atccttatga agttcgtata aggaaaaatt tatttttaca cattctataa agggcacttc
     2221 aatttgaaac agttttagta tctgattaga acgtcacagt atgaaaaaca aatactctta
     2281 tgtgttgaat tctattttca agatcattgt tattctgtta ttaccaaatt ctatcaatgc
     2341 tcaatttggt tcaccattcg tt
//
LOCUS       DAORQT010000250         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9304_length_2358_cov_6.127660, whole genome shotgun sequence.
ACCESSION   DAORQT010000250 DAORQT010000000
VERSION     DAORQT010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9304_length_2358_cov_6.127660"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PK134_07655"
     CDS             <1..381
                     /locus_tag="PK134_07655"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRB38761.1"
                     /translation="NFAISKSTFLTLPIAFNPDVMKKWIEGSFVFELKKGGNVELFDG
                     WVKGEVITFINNESFSYTWKPNTWDKKTKPSLVEIELKPDAAGTRVYIHHSLFPSNDD
                     TDKHRHGWINFVLDPLNDYFIGLM"
     gene            complement(388..1869)
                     /locus_tag="PK134_07660"
     CDS             complement(388..1869)
                     /locus_tag="PK134_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRB38762.1"
                     /translation="MKIKNITTLGDLKKNGYKSRSIKEEMRENLIRKLKSGEKLFEEI
                     QGYEDTVIPQLQTAVLSKHNIILLGLRGQAKTKIARLMTQLLDEYMPIVSGSELNDDP
                     LNPLSRYAINIIEDEGDTTPITWIHRNDRYTEKLATPDVSVADLIGDIDPIKAAAQKL
                     NYSDERVIHYGLVARSNRNIFVINELPDLQPRIQVALFNILQEGDIQIRGFKLRLPLD
                     IQFVFTANPEDYTNRGSIITPLKDRIGSQILTHYPKNIEIAGAITLQESKIEKSQSEN
                     IFIAPLLHQLIEQTAMEARQSELVDQKSGVSARLTISALENLYSTCEQRMLLNHENKS
                     NGRISDLQGIIPSITGRVELVYEGEAEGIQSVALTLMDKAIRKVFAETFTDPEKLKKS
                     KNTNPYSEIINWFEQGNTLSLLHLDTTAQYIKKLDEVHGLKKTVKQFLKNINENEVHL
                     WMEFLLYGLAAFSQISKTALERNTEFKDLMRTVFNFKSMDDEV"
     gene            complement(1873..>2113)
                     /locus_tag="PK134_07665"
     CDS             complement(1873..>2113)
                     /locus_tag="PK134_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38763.1"
                     /translation="KNSFGLDQKIINSVFSLASQCRKQKIPITTFMIATDPYLQQFVR
                     QMTAINKGRAFYSSLNGLGEFVFEDYVKNRKKRVL"
     assembly_gap    2114..2136
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2137..>2358)
                     /locus_tag="PK134_07670"
     CDS             complement(<2137..>2358)
                     /locus_tag="PK134_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38764.1"
                     /translation="ISIKYPKDTLDIIAFGDDAFPIDIKDLPYLQVGPYHTNTTAGLQ
                     LAMELLRRKKTQNKQIFMITDGKPTCIKSG"
BASE COUNT          666 a          406 c          434 g          829 t
ORIGIN      
        1 aactttgcca tttcaaagtc aacattcctt acacttccca ttgcctttaa tcctgatgtg
       61 atgaaaaaat ggatagaagg aagttttgtt tttgaattga aaaaaggagg taatgttgaa
      121 ttgtttgacg gttgggttaa gggcgaagtt attacgttta ttaacaatga atcattcagc
      181 tatacctgga aacccaatac atgggataaa aagaccaaac caagcttagt ggaaattgag
      241 ttgaagcccg atgctgccgg tacaagagtg tatattcatc acagtttgtt tccaagtaat
      301 gacgatacag ataaacacag acatggatgg atcaattttg tacttgaccc gttgaatgat
      361 tatttcatcg gattgatgta ataaagttta aacttcatca tccatagatt taaaattgaa
      421 tactgttcgc attaaatctt taaactctgt gtttctttcc aaagcagttt tgctgatctg
      481 agaaaaggca gcaagtccat acaatagaaa ttccatccat agatgaactt cgttttcatt
      541 tatgtttttc agaaattgtt tcactgtttt tttaagtcca tgaacttcat cgagcttttt
      601 aatgtattgt gctgttgtat cgagatgtaa aagactcaat gtatttcctt gttcaaacca
      661 atttataatt tctgaatatg gattggtatt ttttgatttt tttaattttt cgggatcagt
      721 aaatgtttct gcaaaaactt ttctgatagc cttatccatc aaagttaatg ctactgattg
      781 aatgccttcg gcttcacctt catagaccaa ttccacacgc ccggtgatag atggtattat
      841 gccttgcaaa tcgcttatgc gtccattgct tttgttctca tgatttaata acatcctttg
      901 ttcgcatgtg ctgtacaaat tttcaagtgc agaaatggtc agtcgtgcac tcacaccact
      961 tttctgatca accaactcgc tttggcgggc ttccatggca gtttgctcaa tgagttgatg
     1021 tagtaaagga gcaataaaaa tattttcaga ttgagatttt tcaatttttg attcctgcaa
     1081 ggtaatagct cctgctattt caatattctt tggatagtgt gtaagtatct ggctgcctat
     1141 acggtctttt aagggtgtga tgattgaacc tctgttggta taatcttcgg gattggcagt
     1201 aaacacaaat tgaatgtcta aaggcagacg cagtttgaaa ccgcgaatct gtatatcgcc
     1261 ttcctgcaga atgttgaaca atgcaacttg tattcggggt tgtaaatccg gaagttcatt
     1321 gataacaaaa atgtttctgt tgcttcgggc aactaaacca taatgaataa cgcgctcgtc
     1381 agaatagttt aacttttgtg cagcagcttt aataggatca atgtcaccaa tcaggtcagc
     1441 tacagaaaca tctggagtag ctaatttttc agtgtaacgg tcgtttctat gtatccatgt
     1501 tatgggtgtg gtgtcgccct catcttcaat aatattaata gcatatcgcg aaagcggatt
     1561 cagaggatcg tcatttaatt cagaaccact tactataggc atgtattcat ccaacagttg
     1621 tgtcatcagt cgtgcaattt ttgtctttgc ctgaccgcgt agtcctaaaa gaatgatgtt
     1681 atgtttagat aagacagcag tttgcagttg tggaattact gtatcttcat agccttgtat
     1741 ttcttcaaat aatttttccc ctgatttgag tttacgaatc aggttttctc gcatttcttc
     1801 tttaatagac ctggacttgt atccgttttt tttcaaatcg ccaagtgttg ttatattttt
     1861 aatcttcatt ggttatagaa ctcttttttt acggtttttt acataatctt caaatacaaa
     1921 ttcgcccaat ccattcaggc tgctgtagaa tgcacggcct ttatttattg cagtcatttg
     1981 tcttacaaat tgctgcaaat aagggtcggt agctatcata aaagtagtga taggaatttt
     2041 ttgtttgcgg cattgtgatg ccaacgagaa aacactgtta attatctttt ggtcaaggcc
     2101 aaaactattt ttannnnnnn nnnnnnnnnn nnnnnncccg attttataca agtcggtttg
     2161 ccgtctgtaa tcataaaaat ctgtttgttc tgtgtttttt ttctgcgtaa taattccatg
     2221 gcaagttgta gtcctgctgt agtattggta tgataaggac caacctgcaa ataaggcaaa
     2281 tcttttatgt caataggaaa agcatcatca ccaaatgcaa taatgtctaa agtatcttta
     2341 ggatatttta tactgata
//
LOCUS       DAORQT010000251         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9305_length_2358_cov_6.031698, whole genome shotgun sequence.
ACCESSION   DAORQT010000251 DAORQT010000000
VERSION     DAORQT010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_2358_cov_6.031698"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK134_07675"
     CDS             <1..514
                     /locus_tag="PK134_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRB38765.1"
                     /translation="ASELGLNAKLAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKNK
                     ENAEVCNAIGAHHDEIEMTSLISPIIQVCDAISGARPGARREIVEQYIKRLKDLEALA
                     LSYPGVEKSFAIQAGRELRVIVESEKVSDQQADTLSFDLAQRIQTEMTYPGQVKVTVI
                     REKRAVNYAK"
     gene            534..1808
                     /locus_tag="PK134_07680"
     CDS             534..1808
                     /locus_tag="PK134_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRB38766.1"
                     /translation="MAATEHKEYYRNLITLVTGSAFAQLISLALSPVFSRIYTPHEFG
                     LLAVFMSILSALSVIVCLRYEFAIIPARTGKDAFHLLELCFYSTLLITALTLVGVTIF
                     NLFFSTNAVLSKWIWFLPFMVLTNGSYQAMLSWANRHKSYKVISRYRIINSVVTNTII
                     LGAGLAGFISMGLLAGFVTGGVVASIMLYFNIQKQSEPFTLGFDLTALKNVAKKYEAF
                     PKVNLWQSIADMLLINGVIYFLTAFFSPAVVGLYGLTMRVLQAPFNLIGYSMGQVFIQ
                     DAAHQYNEGKNIQPIIKKSIQRTAFIAIPIVLPLILAGPQLFAFVFGENWRQSGEYAQ
                     ILAPWFFLDFIRTPLGQIPIIMGKQKRQFTITIAGNILMVISLLLGGFYFNNPLLVVT
                     LFTALQSLYLIYVIYWIYSISGVTSEIRDDSR"
     gene            1947..>2358
                     /locus_tag="PK134_07685"
     CDS             1947..>2358
                     /locus_tag="PK134_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38767.1"
                     /translation="MAVPYSKSIAFDWFIQLPGSYKISLFNLVEIISFSTAFFIIRLR
                     VLAGIERRILIALLLVMATRVISLLFAKQYESIQYFSILRFAEVLLGLIILTNLLQSK
                     ANRNIFIAGLACGLLIECFLGLNMVIQTGGHLRGI"
BASE COUNT          677 a          461 c          463 g          757 t
ORIGIN      
        1 ggccagcgaa ttgggattaa atgccaagct tgctaagcgt gcaggtttat tacatgatat
       61 tggaaaagta cccgatgatg agcctgaatt accacatgct attctgggaa tgaaacttgc
      121 cgaaaaaaat aaagaaaatg ccgaagtatg caatgccatc ggtgcccatc acgatgaaat
      181 tgaaatgaca agcctcatca gtcctatcat tcaggtttgt gatgccattt caggcgcacg
      241 cccgggtgca agacgcgaaa tcgttgagca atacatcaag agattgaaag atttggaagc
      301 acttgccctc tcctatcccg gtgtagaaaa atcttttgcc attcaggcag gacgcgaatt
      361 gcgtgttatt gttgaaagcg aaaaagtaag cgaccagcaa gctgatacat tgagttttga
      421 tcttgcacaa agaattcaaa cagagatgac ctatcccgga caagtaaaag ttacggttat
      481 ccgtgagaaa cgtgcggtaa actacgccaa gtaacagcct cgaggcagtt ttcatggcgg
      541 caacagaaca taaagagtac tatcgcaacc tgattacttt agttaccggt agcgcatttg
      601 cgcagttaat tagcctggca ttgtcaccgg tattttcaag aatatacaca cctcatgagt
      661 ttggcctgct agctgttttc atgagcattt taagtgcatt gtctgtaatt gtttgtctgc
      721 gttatgagtt tgccatcata cctgcacgca caggaaaaga tgcatttcac ctgcttgaac
      781 tttgttttta ttcaacattg ctgattacgg cattaacgct tgtaggtgta accatattca
      841 atctgttttt ttcaaccaat gctgtcttgt caaaatggat ttggtttctg ccttttatgg
      901 tgctgacaaa cggttcctat caggcaatgc tcagttgggc aaacagacat aaaagctaca
      961 aagtaatatc acgctacaga ataattaatt cagttgttac caataccatt attctgggtg
     1021 cagggcttgc aggttttatt tcaatgggat tgcttgcagg ctttgttaca ggcggtgttg
     1081 ttgcctccat catgctatac tttaatattc aaaagcagtc tgaaccattt acgcttggct
     1141 ttgatttgac tgcattaaaa aatgttgcaa aaaaatacga agcttttcct aaagtaaatt
     1201 tatggcaatc tattgccgat atgctgctaa ttaacggagt catctatttt ctgactgcat
     1261 ttttcagccc tgctgttgta ggtctgtacg gacttaccat gagggtttta caagctccat
     1321 ttaacctgat aggatacagc atgggacagg tttttataca ggatgcagca catcagtata
     1381 atgaaggaaa aaatattcag cccattatta aaaaatctat tcaacgcact gctttcatag
     1441 ctataccaat tgtgttacca ttaattttag cagggccaca actttttgct tttgtctttg
     1501 gcgaaaattg gcggcagtca ggcgagtatg ctcaaatact tgctccctgg ttttttcttg
     1561 actttatcag aacaccattg gggcagattc ctatcatcat gggcaagcaa aaacgacaat
     1621 tcacaataac aattgccgga aatattttga tggtaatttc tttgttgtta ggtggctttt
     1681 attttaataa tccattgctt gtggtcacct tatttacagc acttcaatcg ctatatctca
     1741 tttatgtcat ttattggatc tatagcattt ctggtgtaac ctcagaaatc agggatgatt
     1801 caaggtaatt ctctacccct gctataacct tatttttatt tttgcagttc aagacaatga
     1861 ctgttactcc caatagcata ccggccaaca acacgtatca actgcttcaa aagattttat
     1921 gcttctgcct ggcaattaca tcttttatgg cagtgcctta ctctaaaagt attgcattcg
     1981 actggtttat tcaactgccc ggaagttata agattagtct ttttaactta gtagaaataa
     2041 taagtttctc aactgctttt ttcataatca ggcttcgggt tttagcaggt attgaaagaa
     2101 gaattctgat tgccctctta ctagtaatgg caacccgagt aatttctctg ctctttgcaa
     2161 aacaatatga atccattcaa tatttcagca ttctgaggtt tgccgaagtt ttattaggat
     2221 taattatcct gaccaacctg ttgcaatcta aagccaacag aaatattttt attgctggat
     2281 tggcatgtgg gttattgatt gaatgttttc ttggcttaaa catggtcatt caaacaggag
     2341 gacatctaag aggcattt
//
LOCUS       DAORQT010000252         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9328_length_2353_cov_5.356397, whole genome shotgun sequence.
ACCESSION   DAORQT010000252 DAORQT010000000
VERSION     DAORQT010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9328_length_2353_cov_5.356397"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK134_07690"
     CDS             complement(<1..186)
                     /locus_tag="PK134_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HRB38768.1"
                     /translation="MSNYLDFEKPIEELYEQLEKTKQIGNKGKIDVNNLLTEIEEKIQ
                     VAKKQIYSNLTPWQRVQL"
     gene            280..1104
                     /gene="ispE"
                     /locus_tag="PK134_07695"
     CDS             280..1104
                     /gene="ispE"
                     /locus_tag="PK134_07695"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRB38769.1"
                     /translation="MLNFPNAKINLGLFITSRFDDGYHALETLFYPINWCDILEITEN
                     KNYSKSQPKVNLYISGLAVDGPTDQNLCSKAYHLLKEKFNLPPVNIFLHKQIPTGAGL
                     GGGSSDGAHTLLLLNKLFDLKLSNEDLSVFALNLGSDCPFFISNKPSFAFSKGEILKP
                     APTVLEGNFIVVIKPSVSVSTAMAYSQVKPDKPNKSLTEILNLPKIQWKDFLKNDFEK
                     TILPQFPQIEKLKTNLYQAGAYYASMSGSGSAVFGLFHDEVKLSFLKDEQHHSAWL"
     gene            complement(1105..1842)
                     /locus_tag="PK134_07700"
     CDS             complement(1105..1842)
                     /locus_tag="PK134_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38770.1"
                     /translation="MQLRLTVIFFLLSFGVIAQDVRYDYVEDLPLRYTNEYSIGATLH
                     SSGWGFMFRKGKNLTVSRKRMYEIEIVSMKHPKEYKSVSYLSERPKTFVFGKMNSLLV
                     MHGGFCYERVLFDKAERRHFEIRWNSTGGPSLGFTKPVYLIFATGNGQRVVEKYDPNN
                     QNHDRQSIYGKAEFTKGLDELKLYPGLFLKSGVMFEYNIMNDGITAVEVGVMLDAYTK
                     TIPIMAFAKNKQVFFNLYGTFMLGRKK"
     gene            1947..2240
                     /locus_tag="PK134_07705"
     CDS             1947..2240
                     /locus_tag="PK134_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRB38771.1"
                     /translation="MFRKTNEESLKEVIEQLLETYKLRDKLNQVKLQRSWDEIMGEMI
                     SKRTEKLILKDHTLYIYLNSAPLKEELSYGREKIVQMLNTALEGDFIKEVVIR"
BASE COUNT          755 a          441 c          378 g          779 t
ORIGIN      
        1 taattgaaca cgttgccacg gtgtcaggtt tgaatagatt tgcttttttg ctacctgaat
       61 tttttcctca atctcagtta aaagattgtt tacatctatt tttcctttat tgcctatttg
      121 ttttgttttt tccaattgtt cgtataactc ttcaattggc ttttcaaaat caagataatt
      181 ggacatgttt tctttttatt tgcaatgaca aaagtagcat aatttgatta tgtctgatga
      241 ttgttaaaac ttatcgaaaa tttatctttg ccggcactta tgcttaattt tccaaatgcc
      301 aaaattaatc tcgggttatt tataacctct cgttttgacg atggttatca tgcattggaa
      361 acattgttct atcctatcaa ctggtgcgat attttagaaa taactgagaa taaaaattat
      421 agcaaatcac aacctaaagt aaatttatat atatcaggat tggcagttga tgggcctact
      481 gatcagaatt tatgcagcaa agcctatcac cttctaaaag aaaaattcaa tcttccacct
      541 gtaaatatat ttctgcataa acaaatacca accggtgctg gacttggtgg cggatcatcc
      601 gatggtgcac acacactact ccttttgaat aaattgtttg atttaaaact gagtaatgaa
      661 gatctctctg tatttgcctt aaatctgggt tctgattgtc ctttttttat ctccaacaaa
      721 ccatcatttg ccttttcaaa aggtgaaata ttaaaacctg caccaacagt attagagggt
      781 aattttatag ttgtaataaa accttctgtt tctgtatcaa cagctatggc atacagtcag
      841 gtcaaacctg ataagcccaa taagagtctt acagaaattt taaacttacc caaaatccaa
      901 tggaaagatt ttctgaaaaa cgattttgaa aaaactattt taccacaatt tccacaaata
      961 gaaaaactaa aaactaacct ctatcaggcc ggtgcttatt atgcttcaat gagtggcagc
     1021 ggctctgctg ttttcggatt gtttcatgat gaagttaaat tgtcttttct gaaggatgag
     1081 cagcatcatt cagcgtggct ttaattactt tttgcggcca agcataaagg tgccataaag
     1141 attaaaaaac acctgcttat ttttggcaaa tgccataata ggaatagttt tggtataagc
     1201 atcaagcatc acccctactt caactgcagt tattccatcg ttcatgatgt tgtattcaaa
     1261 catcacacct gactttaaaa ataagcctgg atatagtttc aactcatcca aaccttttgt
     1321 aaattctgcc ttgccataaa tactttgtct gtcatgattt tgattgttag ggtcatattt
     1381 ttcaaccaca cgttgaccgt ttcctgttgc aaaaattaaa tacacaggct ttgtaaaccc
     1441 taatgatgga ccacctgttg aattccatct tatctcgaaa tgtctgcgtt ctgctttatc
     1501 aaacaaaaca cgctcataac aaaatccacc atgcattacc aaaagagaat tcattttacc
     1561 aaatacaaaa gtctttggtc gttcagaaag atagctgact gacttgtact ctttcggatg
     1621 tttcatgctc acaatttcta tttcgtacat ccttttgcga cttactgtca gattttttcc
     1681 tttgcgaaac ataaaacccc agccgctgct gtgcaaggtt gcaccgattg aatattcatt
     1741 ggtataacgc aagggcaaat cctcaacata atcgtaacgc acatcttgtg ctattacacc
     1801 aaatgataac agaaaaaata ttactgtcag ccttaattgc attgagcaaa aataaattat
     1861 ttttatttca gccgttaaat cagggcttta tcatgctgat tattttttaa acatctaaat
     1921 tactgctgaa aaactaattt tgtatcatgt tcagaaagac aaatgaggaa agcctgaaag
     1981 aggtaattga gcaactgttg gagacatata aactgcgcga caaactcaat caggtaaaac
     2041 tgcaacgcag ttgggatgaa attatgggcg aaatgattag taaaagaaca gaaaagctta
     2101 ttctgaaaga tcatacattg tatatctatc tcaattctgc acctttaaaa gaggaactca
     2161 gttatggtcg tgaaaaaatt gttcagatgc ttaacactgc cctcgaaggt gattttatta
     2221 aagaggttgt tatcagataa gtccaaattc taattagaat gacaaataaa aaagctgcct
     2281 cttttgaagc agcttttatt ttgtgatccc gctggggctc gaacccagga cccctacatt
     2341 aaaagtgtaa tgc
//
LOCUS       DAORQT010000253         2350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9347_length_2350_cov_4.701961, whole genome shotgun sequence.
ACCESSION   DAORQT010000253 DAORQT010000000
VERSION     DAORQT010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9347_length_2350_cov_4.701961"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    359..375
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <376..>2350
                     /locus_tag="PK134_07710"
     CDS             <376..>2350
                     /locus_tag="PK134_07710"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38772.1"
                     /translation="NAQNVVTLAGQSGISGAVNGSGTSATFNNPHGMVCDVAGNLFVA
                     DRNNHLIRKITPAGVVSTFAGSGSAGSSDGIGTAASFFEPWGIAIDQSGNLYVADTKN
                     YLIRKITPTAVVTTVAGAGTSGVTDGPASNAQFGFPTGIAVDASGNIYVSEFMTHVIR
                     KISNGSVTTFAGTAFQSGDANGTGINAKFDHPHSIAIGQSGNLFVTDTWNNKIRKITP
                     AGVVSTYAGSTAGYVDGAASVSKFDNPLGICADASGDIFVGDVNNFCIRKISNGTVNT
                     FAGTAGTAGAANGPLLQASFNSPSCMAYHSSDKYFIGDEGNELIRLINMNQSSNPLTL
                     STLNNVSVFCPSSTSVITASPQGLSSYTFKEGSTVLATNTTGILSLGTLSAGTHLISC
                     TATGLLGNYATTTALSITIVDASTPVISPSGPISICSGGSATLTASPGIAYLWTNGAT
                     TSSINATLPGTYAVSITYTGGCSYQSNSVTVNLNGSFSASISPSAAQVICPGDSVKLT
                     ATAGTSYLWSNGKTTQSIYAKTAANYTVAVTNSFGCTSVSPPVNVSLKSMPATIDASS
                     DSSCSGEGVLLTVVQQNNTTYSWFDQATGGTALATGSSYATAPLAESTTFYVQLNSNG
                     CTSISRFPVLAYIYPALHVDFTESQAVKADGGYA"
BASE COUNT          740 a          443 c          477 g          673 t
ORIGIN      
        1 atttacaaca aggattatat tatgtcctta tcaaaacaaa taacggtttt ttaaaaaaat
       61 cgaagtttgt tattttgaaa tagtatgaat tgattttatt tttcgatcat tttatataca
      121 gattcatcaa aatacatttc acataagtat aaagttacat tgacaattta gtcaaacagt
      181 tgatcttgtc cgtaaattgt tgtttcagag ttcctaatca cattttcaaa gagtaatttt
      241 tttctgatgt aacgcttcaa tggtgtaacc ttactttaaa tagaaagtat aaaagggagt
      301 tcacttttga agaaaaacga atgataagag ccggaatagc aatatgggct gtcatgttnn
      361 nnnnnnnnnn nnnnnccaat gctcagaatg tagttacact ggcagggcag agtggcattt
      421 caggtgctgt caatggctcg ggtacttctg caacgttcaa caatccacat ggaatggttt
      481 gcgatgtggc aggaaaccta tttgttgcag acagaaataa tcaccttatc agaaaaataa
      541 cacctgcagg agtagtatca acatttgcag gatctggtag tgcaggaagt tcagatggga
      601 taggaacagc agcatcattt tttgaacctt ggggaattgc cattgatcag tcgggaaacc
      661 tttatgttgc cgacacaaaa aattatttaa taagaaaaat tacgccaaca gcagttgtta
      721 caacagttgc cggagcggga acttcaggcg ttactgacgg gccggcaagc aatgcacagt
      781 ttggatttcc aacaggtatt gcagtagatg cttcaggaaa tatttatgtg agtgagttta
      841 tgacacatgt tatccgcaag ataagtaacg gtagtgttac cacttttgca ggaacagctt
      901 ttcagagtgg cgatgccaat ggtaccggaa ttaatgcaaa gtttgatcat cctcatagta
      961 ttgctattgg gcagtcagga aatttatttg ttacagatac ctggaataat aaaatcagaa
     1021 aaattactcc tgcaggtgta gtgagcacct atgcaggaag tactgcagga tatgttgatg
     1081 gtgccgcttc tgtgagtaaa tttgataacc ctttgggcat ttgcgcagat gcatcaggcg
     1141 atatatttgt tggtgatgta aataatttct gtatccgtaa aatcagcaat ggcacagtaa
     1201 acacttttgc aggaacagca ggaacagcag gcgcggctaa tggccctttg cttcaggcct
     1261 cattcaattc accatcatgt atggcatatc attcatcaga caaatatttt attggtgatg
     1321 aaggaaatga attgatccga ctcatcaaca tgaatcagag tagtaatcca ctaacacttt
     1381 caacattaaa taatgtgagt gtattttgtc caagttcaac ttctgttatt acagcttccc
     1441 cacaaggact aagcagctat acttttaaag aaggaagtac agtattggca acaaatacaa
     1501 cagggatatt gtctttagga acattatcag caggtacaca tcttatcagt tgtactgcaa
     1561 ctggactatt aggaaactat gcaacaacaa cagcattaag tattacaatt gttgatgcaa
     1621 gcacacctgt aatttctcct tcggggccaa tatcaatttg ctcaggtgga agtgctacat
     1681 tgacagcttc acccggtata gcatacctgt ggacaaatgg agcaacaaca tcttcaatca
     1741 atgccaccct gccaggcact tacgcagtga gcataactta tacaggtggc tgcagttatc
     1801 aaagtaattc agttactgta aacttaaacg gatcattcag tgcatctata tctccatcag
     1861 cagcacaggt tatttgcccc ggtgactcgg taaaattaac agctactgca ggaacatcat
     1921 acttgtggtc taatggaaaa acaacacaaa gcatttatgc taagacagcc gcaaactaca
     1981 ccgtagctgt gacaaacagt tttggatgta cttctgtttc tccaccagta aatgtttcgt
     2041 tgaaatctat gccggcaaca attgacgcaa gcagcgattc atcatgcagc ggtgaaggtg
     2101 ttttgttaac agtagttcag caaaacaaca caacctatag ttggttcgat caggcaacag
     2161 gaggcacagc acttgcaaca ggcagcagct atgctactgc acctcttgca gaaagcacca
     2221 cattttatgt tcaactaaat tctaatggct gcacaagcat ttcaagattt cctgtacttg
     2281 catacatcta tcctgcttta catgttgatt ttacagaaag ccaggctgtg aaagcagatg
     2341 gtggttatgc
//
LOCUS       DAORQT010000254         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9357_length_2348_cov_7.324466, whole genome shotgun sequence.
ACCESSION   DAORQT010000254 DAORQT010000000
VERSION     DAORQT010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9357_length_2348_cov_7.324466"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PK134_07715"
     CDS             <1..1188
                     /locus_tag="PK134_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38773.1"
                     /translation="LYHIQGEYRYPNKWVDGVVGGNYRLYKPNSDGTIFSDTSYVHYN
                     ISANNDTLSIDTLRNKLSVYEFGVYAGLEKKILKDKLKLNATVRFDKNQNFKLLVSPA
                     GSVVYTPNNNHVIRVSFSSAVRNPTLTDQYLFYRVGRATLLGNINGYTNLLTPESVTE
                     FYDSSKSSFKFLNFFNIDPVRPEKVKTIEAGYRATLFNRLYVDLNGYYSWYRDFIGYQ
                     IGVDADTFSVNTFGGTIYDLVFNRVVRVASNAQSEVTTQGVSVGLNYYIGKYFALTGN
                     YSWNKLNKKGTDDPLIPAFNTPENKYNIGFNGRDIKGVGFNVNYKWVQGFRYEGSPQF
                     TGEIPSYDMVDAQVNYHLKEAGTTFKLGAMNLFNNKHYEIYGGPKVGRMVYFSILFEV
                     DKL"
     gene            1219..1707
                     /locus_tag="PK134_07720"
     CDS             1219..1707
                     /locus_tag="PK134_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRB38774.1"
                     /translation="MKTSAKSVDEILSNVQEDRKEVFQKLHQVILKNLPKGFEAAISY
                     GGLGYVVPHSIYPDGYHCKPVEPLPFAGIASQKQSINFYHMGMYSDPVLLKWFVSEFP
                     KHSNQKLDMGKSCVRFKKMDDIPYKLIGELMKKMTVKEWIQKYESAYKPKPKEVKTKK
                     GK"
     gene            complement(1778..>2348)
                     /locus_tag="PK134_07725"
     CDS             complement(1778..>2348)
                     /locus_tag="PK134_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38775.1"
                     /translation="SYAQLYLSEGDTAKALEVLSRGLSRFPEEKSILITQTNIYISSG
                     KTAEALSAINMAIEKDPTNANLYFAKGTLVEKTEKSKDAAIAAYKKAIELKADYFDAY
                     YNLGALYFNDGAHLANEANSIKDNNQYAKAKAVFEAKFKEAKPYLEKAWELNPKDQST
                     MASLKQLYATINDTVNYAKVKAALDAETK"
BASE COUNT          729 a          375 c          464 g          780 t
ORIGIN      
        1 ctttatcaca tacaaggtga atatcgttat ccaaacaaat gggttgatgg tgtagtagga
       61 ggaaactatc gtctttacaa acctaattct gacggaacaa ttttttctga taccagttat
      121 gtgcattata atatttcagc aaacaacgat acattatcaa tagatacttt acgcaataaa
      181 ctttctgttt atgaatttgg tgtttatgca ggattggaaa agaaaattct gaaagacaaa
      241 ttaaaactca acgctacggt acgttttgat aaaaatcaaa attttaaact gttagtttct
      301 cctgcaggtt ctgttgttta tacaccaaat aacaatcatg tcatcagagt ttcgttttca
      361 tctgctgtaa gaaatcctac acttacagat caatatttat tttatcgggt aggaagggct
      421 actctgcttg gaaacatcaa tggctacacc aaccttttaa ctcccgaatc ggtaactgag
      481 ttttatgata gcagcaagtc atcatttaaa tttttgaact tctttaacat tgatcctgta
      541 agaccggaaa aagttaaaac cattgaagct ggttatcgtg caacactgtt taaccgttta
      601 tatgttgatt tgaatggata ttattcatgg tatcgtgatt ttatcggata tcagataggt
      661 gttgatgccg acactttttc tgtaaacact tttggtggta ctatttacga tttggttttt
      721 aatcgtgtag tacgtgtggc ttcaaatgca caaagtgaag ttactacaca gggagtttct
      781 gtcggattga attattatat cggaaaatat tttgcgctta ctggaaatta ctcttggaat
      841 aaactgaaca aaaagggcac tgatgatcca ttgattcctg ccttcaacac accggagaat
      901 aaatacaata ttggtttcaa cgggcgcgat ataaaaggtg ttggttttaa tgtgaactac
      961 aaatgggttc agggttttag gtatgaaggc tcaccacaat ttacaggcga aattccttcg
     1021 tacgatatgg ttgatgcgca agttaattat catctgaagg aggcaggcac aacttttaag
     1081 ctgggtgcaa tgaacttgtt caacaataag cactatgaaa tttatggcgg acccaaagtt
     1141 ggcagaatgg tttacttcag tattttgttt gaggtggata aattgtaatt tttgaaactg
     1201 ttttaaaaaa gataaggaat gaaaactagt gcaaaaagtg tagatgaaat tttgtcaaac
     1261 gtacaggaag ataggaagga agtttttcaa aaactgcatc aggtaatatt aaaaaattta
     1321 ccaaaaggtt ttgaggcagc aatcagttat ggtgggttag gttatgttgt gccacattca
     1381 atttatcctg atggctatca ttgcaaacct gttgagcctt tgccttttgc aggaatagct
     1441 tctcaaaagc aatctataaa tttttatcac atgggtatgt attccgaccc tgttttgcta
     1501 aaatggtttg taagcgaatt tccaaaacac agcaatcaaa aattggatat gggaaaaagc
     1561 tgtgttcgtt ttaagaagat ggacgatatt ccatacaagc tcattggtga gttgatgaaa
     1621 aaaatgacgg ttaaagagtg gattcaaaaa tatgaatcag cctacaaacc taaacctaag
     1681 gaagtgaaaa cgaagaaggg aaaataggat aatgcaagta agagataaaa gcaaaaaagc
     1741 cccgcattgc ggggcttttt ttatgtgttc aaaaatttta ttttgtttct gcatctaatg
     1801 ctgctttcac ctttgcgtag tttactgtgt cattgatagt ggcataaagt tgtttcagag
     1861 aagccattgt actctgatct ttaggattca actcccatgc tttttcaaga taaggttttg
     1921 cttctttaaa ctttgcttca aatactgcct tagcttttgc atattgatta ttgtctttga
     1981 tgctattagc ttcattggct aaatgcgcac catcgttaaa atagagtgca cccaagttgt
     2041 aataggcatc aaaataatct gctttaagct caattgcttt tttgtaggca gcaatggcgg
     2101 catctttact cttttcagtc ttctctacta atgtaccttt tgcaaaataa aggttggcat
     2161 tggtaggatc tttttctatt gccatattta tggcgctaag tgcttcagca gtttttcctg
     2221 agctgatata aatgttcgtc tgtgtaataa gaattgattt ctcttcaggg aagcgtgata
     2281 atccacgtga caatacttca agtgcttttg ctgtgtcgcc ttcggaaaga tacagttgtg
     2341 catagctg
//
LOCUS       DAORQT010000255         2340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9403_length_2340_cov_6.432823, whole genome shotgun sequence.
ACCESSION   DAORQT010000255 DAORQT010000000
VERSION     DAORQT010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9403_length_2340_cov_6.432823"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK134_07730"
     CDS             <1..439
                     /locus_tag="PK134_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HRB38776.1"
                     /translation="TIKEIYHKFKDAEVHQTGKTGRKMTMIQAIFQIMVIDVVFSFDS
                     IITAVGLSNQIVIMVGAVIGAMLVMIAFAPYVSDFINKYPTLKMLALAFLVVIGVILV
                     VEALHIHFDKSYVYVALGFSLLVEMLNIRMRSLSHRKKVDQQK"
     gene            436..957
                     /locus_tag="PK134_07735"
     CDS             436..957
                     /locus_tag="PK134_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB38777.1"
                     /translation="MSTSIGFDSLKLNRQLLNAIGELGYETPTEIQQKVIPPALSGQN
                     IIGIAQTGTGKTAAFILPLLRTLNYAQGNEPRALILAPTRELVIQIGAVANQMGKYVD
                     LRIVTVYGGKGFSDQKKKLEAGCDIVVATPAQAMELYLSGHLILKKIKHFVMAYGLWF
                     YRTDLQLTRSVAS"
     gene            1022..>1501
                     /locus_tag="PK134_07740"
     CDS             1022..>1501
                     /locus_tag="PK134_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HRB38778.1"
                     /translation="MEFPVFIDVVPQVKTAAKIEQYLYTVNSFQTKLNLLNFLLSDKE
                     NFNKVIIFCKSKKNANRIAEVIQMNYGADGINVLHGDKTQQTRLNAINRFREEEIRLL
                     IATDVAARGIDVPGVSHVINFDVPLVYEDYIHRIGRTGRAFASGLSITFVEPQDEWHI
                     "
     assembly_gap    1502..1557
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1734..>2340
                     /locus_tag="PK134_07745"
     CDS             1734..>2340
                     /locus_tag="PK134_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB38779.1"
                     /translation="MRWVYLLVLASMWGSSFILMKRGLNSFSANQVAAIRMFISCMVM
                     LPFIASHLKDIPKKLWLFVMATGLLGNGIPAYLFTHAETGISGSMAGMLNSLTSMFTL
                     VVGSIFFATQFSKRHLLGVIIGFAGAASLIWLNADDAQSAEPWKGVYVLIATVCYAFS
                     VNILRNKLASINAVTLTGAALLFAGIPSGIYLFSSDFVSRFD"
BASE COUNT          705 a          380 c          484 g          715 t
ORIGIN      
        1 aactattaaa gaaatttatc ataaatttaa agatgcagaa gtgcatcaga ccggtaaaac
       61 tggacgcaaa atgactatga tacaggcaat ttttcaaatt atggtaatag atgttgtatt
      121 ttctttcgac tctataatca ctgccgtagg tctttctaat cagatagtaa ttatggttgg
      181 tgctgtaatt ggtgctatgc tggtgatgat tgcttttgct ccttatgtga gtgattttat
      241 caataaatac cccacattaa aaatgctggc attggcattt ctagtagtta ttggagttat
      301 acttgttgtt gaagcattgc acattcactt cgacaagagt tatgtttatg tggctttagg
      361 tttttcgttg ctggtagaaa tgctcaacat cagaatgcgt agcctgagtc atcgcaaaaa
      421 ggttgaccaa caaaaatgag tacttcaatc ggttttgatt cgttgaaatt aaaccgccag
      481 ttattgaatg caattggaga attaggctat gaaacgccta cagaaattca gcaaaaggta
      541 atacctcctg cactttctgg acagaatata attggaatag cacaaaccgg aacaggaaaa
      601 acagcagcat ttattcttcc tcttcttcgt actttaaatt atgctcaagg caatgagcca
      661 agagcgctca tccttgctcc tacccgtgag ttagtgattc aaattggtgc ggttgcaaat
      721 caaatgggta aatatgttga cctaagaatt gtgactgttt atggtggcaa aggtttttct
      781 gatcagaaga aaaaacttga agcaggttgc gatattgttg ttgccacgcc tgcacaggca
      841 atggaacttt atctttcagg tcatcttatt ctaaaaaaaa taaaacactt tgtaatggct
      901 tatggacttt ggttttacag gacagattta cagcttacta gaagtgttgc ctcgtaaaag
      961 gcaaaacatg ttgttttctg caacattctc cgaaagggta aaaaaaatat cggatgactt
     1021 tatggagttt cctgttttta ttgatgttgt tccgcaggtt aaaacagcag caaaaattga
     1081 acagtattta tataccgtaa acagttttca gacaaaattg aatctgctga actttttact
     1141 ttcagacaaa gagaatttta ataaagtaat cattttctgc aagtcgaaaa aaaacgctaa
     1201 ccgcattgcc gaagtaatac agatgaatta tggagccgat ggtataaatg ttttgcatgg
     1261 cgacaagaca caacagacaa gattaaatgc cattaaccgt ttcagagagg aagaaataag
     1321 gttattgata gctactgatg tggctgcacg aggtattgat gttcccggag tttcgcatgt
     1381 aataaatttt gatgtgcctt tggtttacga agattacatt catcgtattg gccgtaccgg
     1441 tcgtgcattt gcatcaggac tttctatcac ttttgttgag ccgcaagacg aatggcatat
     1501 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact
     1561 taacattcct gagtctgttg aagtcataca acccggaaga gaagaaacgc agcttcgcga
     1621 tatggaaatt gaccgtcaga aaagaaagga caatccggaa tttaaaggtg catttcacga
     1681 gcggaaaaac aaatcaggca agaaaggcag acatgcaggc aggaagtaaa gtaatgcgtt
     1741 gggtttactt gctggtgctt gcatctatgt ggggcagttc atttatttta atgaaacgcg
     1801 gactgaatag tttttctgca aatcaggttg ctgcaataag aatgtttatt tcatgtatgg
     1861 tgatgcttcc atttattgcg tcacatttaa aggacatacc aaaaaaactg tggttatttg
     1921 ttatggcaac aggcttatta ggaaatggta ttcccgccta cctttttact catgcagaaa
     1981 caggcatcag tggttctatg gccgggatgt taaactcatt aacctctatg tttactcttg
     2041 tggtgggaag tatatttttt gcaactcaat tcagtaaaag gcatttatta ggagtgataa
     2101 ttggatttgc gggagcagcc tcattgattt ggctcaatgc agacgatgcg caaagtgcag
     2161 aaccatggaa aggtgtttat gttcttattg caacagtatg ttatgcattt agtgtaaaca
     2221 tcctcagaaa taaattagca tcaataaatg cagttacact aacgggcgct gcattgcttt
     2281 ttgcaggaat accaagtggc atctatcttt tttcaagtga ttttgtttcg cgttttgata
//
LOCUS       DAORQT010000256         2339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9415_length_2339_cov_5.334063, whole genome shotgun sequence.
ACCESSION   DAORQT010000256 DAORQT010000000
VERSION     DAORQT010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_2339_cov_5.334063"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK134_07750"
     CDS             complement(<1..601)
                     /locus_tag="PK134_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRB38780.1"
                     /translation="MVFAIDPNFRTNGYFYLYYVVDRHYLLHYGTGSYSPTTNEYDAA
                     TICRITRYTANSATNFTTTVPGSRLILLGETKKTGIPVLYDTHSAGTLLFATDGTLIV
                     SAGDGASPATADSGQVASILSYWAQALADSIITPQQNVGAYRAQLIQSLNGKILRLDP
                     ATGDGIPSNPFYDSTDARSPQSRVWALGLRNPFRISLKPG"
     gene            complement(585..872)
                     /locus_tag="PK134_07755"
     CDS             complement(585..872)
                     /locus_tag="PK134_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38781.1"
                     /translation="MKHYYFTFILIIFLSLNHPLKAQVLPPQFTDQLVSTGWDFVEGY
                     TEDSTGQKYVWEKAGLVWVVDTNGVKIPQPLIDISEEVGNWRDHGMNGFCN"
     gene            1227..2312
                     /locus_tag="PK134_07760"
     CDS             1227..2312
                     /locus_tag="PK134_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3836 domain-containing protein"
                     /protein_id="HRB38782.1"
                     /translation="MELPFQAEGSNICIPEILLEDIHYRNMSAATNCLIDSFYYSFYV
                     NSEEQRAYYTYDAMNNLSILLTQQLSNGSTVWENFNRNTYTYDANKNVTSFLFQQWDN
                     NTSVWKNSTLTTYSYDGNSNRTNYLYQLWDNNTSAWKNSTKISYTYNSGNILTAQQSQ
                     QWDNNTSAWKNKTKGIYTYSSNLNLIDYLSQQWDISTTAWKNYTKSTYTYDAHNNQTS
                     NLTQRWDSSSTWKNSIKSTYSYNANNKLISYVYQQWDNTANAWKNGSKSTYSYDANQN
                     LINLVTQLWDNVSSSWKNNAKNIYTYDSNNNKLSDLSQLWSSTSQTWASQYGYLYYYS
                     CAPTSISEITPSSFTVYPNPSNGIFVF"
BASE COUNT          797 a          404 c          416 g          722 t
ORIGIN      
        1 tccccggttt aagtgaaata cgaaatggat ttcttaaccc caatgcccaa actcttgatt
       61 gaggagaacg tgcatcggta ctgtcataaa atggatttga agggattcca tcacctgttg
      121 caggatctaa tcgtaatatt tttccattca aggattgaat tagttgtgcc cgataagcac
      181 caacattttg ttgtggtgtg atgatactat ctgctaatgc ctgcgcccag taactaagaa
      241 tagaagcaac ttgtccgcta tctgctgtag caggcgaagc accgtcaccg gccgatacaa
      301 tcaatgttcc gtcagtagcg aacaataatg ttccagcact atgtgtgtca tacaaaacag
      361 ggattcctgt tttcttagtc tcacctagta aaatgagtct gcttccgggt actgttgttg
      421 taaagtttgt tgcggaattt gctgtatatc ttgttatgcg acaaatagta gctgcatcat
      481 attcatttgt tgttgggcta tagctccctg tgccatagtg taggagatag tgcctgtcaa
      541 caacatagta taaataaaag taaccgttag ttctgaaatt ggggtcaatt gcaaaaacca
      601 ttcattccat gatcgcgcca attacctact tcttcactaa tgtcaattaa aggttgtggt
      661 attttaacgc cattggtatc aacaacccaa actaatcctg ctttttccca aacatatttt
      721 tgtcctgttg aatcttcagt ataaccttct acaaagtccc agcctgtgct gacaagctga
      781 tcagtaaatt gtggaggaag tacctgtgct tttaatggat gattgagtga aagaaaaata
      841 attaatataa aagtaaagta gtaatgtttc atgatgtcgg tttataacga aaaagcagta
      901 gcaaatataa atgactgaaa aatatcaggc atgtaaaatc tctttaagtt ttccacttgc
      961 aatccatgag tatgaagaat gttaaattat tcttagtcaa cgataaattc gttttttaat
     1021 aagtgtacaa tagaagtaaa tgcaaccttt ttttagaagg tcagtataaa aggaagttca
     1081 aaactcatgt attatttaaa tatttaatgg aaatgaagaa aacatttacc tcatctgtag
     1141 tcatgtttgc agttgtattt ttttctggca atataacagt acaagctcaa agtaaatcaa
     1201 gtcatagtca taatcatgca gtgcaaatgg agctaccttt tcaagctgaa ggcagtaata
     1261 tttgtattcc ggagattctt cttgaagata ttcattatcg taatatgagc gcagcaacta
     1321 actgtttgat tgatagtttt tattactcat tttatgttaa ctcagaggag caaagagcat
     1381 attatactta cgatgctatg aataatttgt caattctgtt gactcagcaa ttgagtaatg
     1441 gctctacagt gtgggaaaac tttaatagaa atacatacac ttatgatgct aacaaaaatg
     1501 tgacaagctt tttgtttcaa caatgggata acaatacttc tgtatggaaa aacagtacgc
     1561 ttacaacata cagttatgat ggaaatagca accgtacgaa ctatctgtat caactatggg
     1621 ataacaatac atccgcttgg aaaaactcta caaaaatcag ttatacatat aactcgggaa
     1681 atatcttgac cgcccaacaa tcacagcaat gggataataa cacttcagca tggaagaata
     1741 agacaaaagg tatctacaca tatagtagca atttgaatct gattgattat ttgagccagc
     1801 aatgggatat aagcaccact gcatggaaaa attacacaaa atcaacttat acctatgatg
     1861 ctcataacaa ccagacaagc aatcttactc aacgatggga tagctcatca acatggaaga
     1921 atagcataaa atcgacttac agttataatg ccaataataa actaataagt tatgtgtatc
     1981 aacaatggga taatactgcc aatgcatgga aaaatggttc aaagagcacc tactcttatg
     2041 atgccaatca gaatctgata aatcttgtta cacagttgtg ggataacgtt agttcatcgt
     2101 ggaagaataa cgctaagaat atttacactt atgattctaa taacaataaa ttaagcgatt
     2161 tatctcagtt atggagtagc acatctcaaa cttgggcaag tcaatatggg tatctctatt
     2221 attatagttg tgctccgact agtatatcag aaattactcc aagtagtttt acagtttatc
     2281 ccaatccatc aaatggaatt tttgtatttt aaacagacaa agtgctaatt ggtacaatt
//
LOCUS       DAORQT010000257         2332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9445_length_2332_cov_6.017567, whole genome shotgun sequence.
ACCESSION   DAORQT010000257 DAORQT010000000
VERSION     DAORQT010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9445_length_2332_cov_6.017567"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK134_07765"
     CDS             <1..543
                     /locus_tag="PK134_07765"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB38783.1"
                     /translation="NALNSINQKKQASTNANSHILGFTYTEPLTNKIKVEFSYDYMQY
                     KSSQNKLSLNKIGDEYSQIDPLLTNDFLNIKKINRLGLKFIYEVKKLRFTLGTKARNV
                     FVRNDNLFTNHQIIQNFNNILPFSTVRYKFSDNRRIELQYTTTSANPTINQLQPITDN
                     NNPNFINIGNPDQLLTITLR"
     gene            540..1283
                     /locus_tag="PK134_07770"
     CDS             540..1283
                     /locus_tag="PK134_07770"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB38784.1"
                     /translation="MNYNSWKAISGKYTWISFGYNYINNNFTNFTTYDSIGRTVSKTV
                     NVDGNYNLNSSFGTNLPFFSKKLVISPYVWVSYRNNKSFINGLMNKTQDFRSNLNLQF
                     QLDFEKVESSISGYYNYNYTEATLNINSTKPYSSQGFSGSINIKLPAKFIMETNADYT
                     INSKRSEGYNVNYLIWNANLSKSFFKQENVILGLFAYDILNQNTNVDREINSNIITDS
                     KTNIITRYFLIKVTYKFNSTHSKEEDEGF"
     gene            1270..2001
                     /locus_tag="PK134_07775"
     CDS             1270..2001
                     /locus_tag="PK134_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRB38785.1"
                     /translation="MKVFKKCISLFSCCMLVLNFSFGQINYGKITYERKTNLYKRFKS
                     DSDVKEWLKEEDKNKIDIFELYFNDTASVFKPQESNLVERMSWATNKNVVYQNFKTNT
                     RFTEKKIWGEQFYLIDSVRKFTWKITDSKRNICGFQCRKAIWQVNDSTRIYAWFCSEI
                     IPSIGPESILGLPGAILGLATEDGGVIYFAKNVEILKPDATLLAPKKNKQKIYTTDEL
                     KTKIEKDFGKEKWGKSLLYNQFAIW"
     gene            complement(2124..>2332)
                     /locus_tag="PK134_07780"
     CDS             complement(2124..>2332)
                     /locus_tag="PK134_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38786.1"
                     /translation="VSYALTVGMSVFNFIWVYMLWGFVVAPYLIINSILLIALFPLFF
                     RYSRSLFLAMVFKIEEAARKRQRH"
BASE COUNT          915 a          361 c          329 g          727 t
ORIGIN      
        1 aatgcattaa actctattaa tcaaaaaaag caggcctcaa caaatgcgaa tagccacatt
       61 ttaggattta cttatacaga gcctttaact aataaaataa aggttgaatt ttcttatgac
      121 tacatgcaat acaagagcag ccaaaataaa ctgtcgctaa acaaaatcgg agatgaatac
      181 tctcaaattg accctttact taccaatgat tttctaaaca ttaaaaaaat taatcgttta
      241 gggttaaagt ttatttatga agttaaaaaa cttcgtttca ctttaggaac taaagctaga
      301 aatgtttttg ttaggaacga taatctcttt acaaatcatc agattataca aaacttcaat
      361 aacatcttac ccttttcaac tgttcgctat aagttttctg acaacaggag aattgaattg
      421 caatatacaa ctacgtctgc aaatcctaca attaatcaac ttcagccaat aacagataat
      481 aacaatccaa actttattaa tatcggcaat cctgaccaac ttttaaccat aacattaagg
      541 tgaattacaa ttcttggaaa gcaataagtg gtaaatacac atggattagc tttggttata
      601 attatattaa caataatttt acaaatttta ctacctatga tagtattggc agaactgttt
      661 caaagacagt taatgtagat ggcaactata atctgaacag ctctttcgga acaaaccttc
      721 ctttcttttc aaaaaaactt gtaatttcac cttatgtttg ggtatcttac cgaaataata
      781 agtcgtttat caatggctta atgaataaaa ctcaggattt caggagtaat ttaaatttac
      841 aatttcaact tgattttgaa aaagtagaat catcaatttc tggctattac aactataatt
      901 atactgaagc aacattaaac ataaatagca ccaaacctta ttcatcacaa ggctttagtg
      961 gaagtataaa tattaaatta ccggctaaat ttattatgga aacaaatgcc gattatacta
     1021 taaattcaaa acgcagcgaa ggttacaatg tcaactatct gatatggaat gccaacctgt
     1081 ctaaatcctt ctttaagcaa gaaaatgtta ttttgggatt gtttgcttat gatatattaa
     1141 atcaaaacac aaatgttgac agagaaatca acagcaatat tattactgat agtaaaacaa
     1201 atatcataac acgctatttt ttaataaaag taacttataa gtttaacagt acacattcta
     1261 aagaagaaga tgaaggtttt taaaaaatgc atttcattgt ttagttgttg catgctcgta
     1321 ctgaattttt cattcggcca aataaattat gggaaaatta cctatgaacg taaaacaaat
     1381 ctatataaac gattcaagag tgatagtgat gtaaaagaat ggctgaaaga agaagacaaa
     1441 aataaaattg acatttttga attatatttt aatgatactg catcggtttt taaaccacaa
     1501 gaaagtaatt tagtggaacg tatgagttgg gcaacaaata aaaatgtggt gtatcaaaac
     1561 tttaagacaa atacacgttt tactgaaaag aaaatctggg gtgaacaatt ttatttaatt
     1621 gattccgtga gaaagtttac ttggaaaatt acagatagta aacgcaatat ttgtggcttt
     1681 caatgtagaa aagctatatg gcaagttaat gacagtacca gaatttatgc atggttttgc
     1741 tctgaaatca ttccctccat tggacctgaa agtatattag gactaccggg tgcaatactt
     1801 ggattagcaa ctgaagatgg tggtgttatt tattttgcga aaaatgtaga gattctaaaa
     1861 cctgatgcaa cattgttggc acccaaaaaa aacaaacaga aaatttatac aactgatgaa
     1921 cttaaaacca aaattgagaa agactttgga aaagagaaat ggggaaaatc tttgttatat
     1981 aaccaatttg caatttggta aaatctaaaa agaagtcatt tattttatct gacaacatct
     2041 taaatcaaac acaattaatt tgcattctga cattcatttt aatgctctat ttcttcactc
     2101 acaaattaca cttacaaaaa tattcaatgc cgttgtcttt tccttgcagc ttcttcaatt
     2161 ttaaaaacca ttgccaaaaa taaacttcgt gaataacgaa agaaaagtgg aaacaatgct
     2221 atcaataaaa tactatttat aattaaatag ggagcaacaa caaatcccca taacatatac
     2281 acccaaataa aattaaatac cgacatgcca acagttaagg catagcttac at
//
LOCUS       DAORQT010000258         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9494_length_2325_cov_6.688546, whole genome shotgun sequence.
ACCESSION   DAORQT010000258 DAORQT010000000
VERSION     DAORQT010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9494_length_2325_cov_6.688546"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK134_07785"
     CDS             <1..538
                     /locus_tag="PK134_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRB38787.1"
                     /translation="YSNYDMTCASRTLRFHTFLKVTNLKNKLVTIVRVNDRGPYAKNR
                     IIDLTEQAARIIGSYKHGITKVKLEIINPPENADSLEKYFLQEQVIDAEGKVVNPTGY
                     TISIWRTRDFDHALLLTKYLQQEEYIQSFYVGKKNQNGRPLYHILVLNISTQEEAVKL
                     KDFWERKGFMRVRMLEKF"
     gene            complement(540..>2325)
                     /gene="pbpC"
                     /locus_tag="PK134_07790"
     CDS             complement(540..>2325)
                     /gene="pbpC"
                     /locus_tag="PK134_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRB38788.1"
                     /translation="LYLDKLPADLSLAECVTLCIIPNRPNSLRPGYDNDLIRRERNKW
                     LTRFEKNKVFKKEIINDALNEPVEMKRQAAPALITQLAYRLKEQYPTIENIHSTLQAP
                     LQHKVQSLVYNYSKRKSLQSIYNASAIVIDNKNHKVVAYIGSADFKDQLHHGQVDGVN
                     AVRSPGSSLKPLIYAMAFDKGLATPMTVINDVPVNYMGYNPENYNSKFNGRVTVDFAL
                     SHSLNIPAVKILNQLGVPTVTQALIKCGFTSIEKSKNKLGLSLALGGCGVRLFELTNL
                     YAAFANSGVYQKLKYVEADSLNNDTIRIMSPGAAYLTTEILSKITRPDLPDNYANLLR
                     IPHIAWKTGTSYGRRDAWSIGYNKRFTVGVWLGNYNGNGVAELSGASVATPLLFDIFN
                     AIDYDSDSTWFSPPADVNFRLACSETGQLPGVNCANQIMDEFLPGISSNAICTHVKEY
                     IISPDEKISYCTSCMPAAGYKKKLYPVPDAEYLAFLKSENISLNLPPPHNPLCTRVFT
                     GQPPKIVSPNMQSEYLLDLKGGDKIMLQCQASADVQNVYWYINDLFYRAASSNEQLFC
                     TPPKGRVKISCADDMGRNSDIFIKIEQM"
BASE COUNT          736 a          448 c          424 g          717 t
ORIGIN      
        1 gtacagcaat tatgacatga cctgtgccag caggacatta aggtttcata cttttttaaa
       61 ggttacaaac ctgaaaaata aattggtaac tatagtacgt gttaatgaca gaggtcccta
      121 tgcaaaaaac cgcatcattg accttactga acaggctgca cgtattatcg gaagctataa
      181 acatggcata acaaaagtta aactagaaat aattaacccg cctgaaaatg ctgactctct
      241 tgaaaaatat tttttgcagg aacaagttat tgatgctgaa ggtaaggttg ttaatccaac
      301 cggttacacc atcagtattt ggcgcacacg cgattttgat catgcgcttt tactgacaaa
      361 atatttgcaa caggaagaat acattcagtc tttttatgtt ggcaaaaaaa atcagaatgg
      421 cagaccactt taccatattt tagtcttaaa catttctaca caggaagaag ctgttaagtt
      481 gaaagatttt tgggagcgaa aaggttttat gcgtgtaagg atgctggaaa aattctgaat
      541 tacatctgtt ctattttaat aaagatgtcg ctgttgcgac ccatatcatc agcacatgaa
      601 attttaacac gaccctttgg tggcgtacaa aacagttgct cattagatga agcagcacga
      661 taaaacaaat cattaatgta ccagtaaaca ttctgcacat cagcagatgc ctgacattga
      721 agcataattt tatctcctcc ttttaaatca agcaaatatt cactttgcat attgggagaa
      781 acaatttttg gtggctgccc ggtaaaaact cttgtgcata aaggattatg cggtggtgga
      841 agattaagac tgatgttttc actttttaaa aatgccaaat actctgcatc tggaaccgga
      901 taaagtttct tcttataacc tgcagcaggc atgcatgatg tgcagtaact tattttttca
      961 tcaggtgaaa tgatatactc ctttacatga gtacaaattg cattggatga aataccgggt
     1021 aaaaactcat ccataatctg atttgcgcaa ttgacaccgg gaagctgacc tgtttcgctg
     1081 caagccaatc tgaaatttac atcagcaggc gggctgaacc atgttgaatc actatcataa
     1141 tctattgcat taaaaatatc aaagagcaac ggagttgcaa ccgatgcacc actaagttcg
     1201 gcaacgccat taccgttata attcccaagc cacacaccta ctgtaaacct cttgttgtag
     1261 ccaatgctcc atgcatccct cctaccatac gatgtaccgg ttttccatgc aatatgagga
     1321 atacgaagta agttggcata attatcaggt aaatcaggtc gtgtaatttt actgagtatt
     1381 tctgtagtca gataggctgc accgggagac attatcctta tagtgtcatt attcaatgaa
     1441 tccgcttcaa catattttaa cttctgatag actccactat ttgcaaaggc agcatacagg
     1501 ttggtgagtt caaaaagacg cacaccacaa cccccaagtg ccaaagacaa ccccaattta
     1561 tttttactct tttcaattga agtgaaacca catttaatca atgcctgtgt gacagtaggt
     1621 acacccaatt gattcagaat ttttactgca ggaatattca gtgaatgtga caaagcaaaa
     1681 tctacggtaa ctctgccatt aaattttgaa ttgtagtttt caggattata acccatataa
     1741 tttacaggca catcattaat tactgtcata ggtgttgcta aacctttgtc aaaagccatt
     1801 gcatagatta atggtttgag gctactgccg ggagaacgaa cagcatttac accatcaacc
     1861 tgtccatgat gtaactgatc tttaaaatct gcagagccaa tataggctac tactttatga
     1921 tttttattgt caatcacaat tgctgatgca ttgtaaatac tttgcagtga cttacgctta
     1981 ctgtagttat acactaatga ttgtacttta tgctgtaatg gtgcttgtaa agtagaatga
     2041 atattctcaa tggttggata ctgttccttt agcctatatg ctaattgtgt aattaatgca
     2101 ggtgcagcct gtcgcttcat ttcaacaggt tcatttaatg catcgttgat gatttctttt
     2161 ttaaaaactt tattcttttc aaatcttgtc agccatttgt ttctttcgcg cctgatgaga
     2221 tcattatcat aacccgggcg cagagagttt ggtctgttag gaataataca caatgttaca
     2281 cactctgcaa gagataagtc tgcaggcaat ttgtcgagat acaaa
//
LOCUS       DAORQT010000259         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9566_length_2314_cov_7.577689, whole genome shotgun sequence.
ACCESSION   DAORQT010000259 DAORQT010000000
VERSION     DAORQT010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_2314_cov_7.577689"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PK134_07795"
     CDS             <1..1389
                     /locus_tag="PK134_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB38789.1"
                     /translation="LGFYGGATQYNGDMGQGFYKTNQPFYGFGAISVGYYLGKYADLQ
                     IMGTTGEAGFIEDQVNRFRIKMTTGSLHLRFHFTKPEAAIRPFLFAGAGIIVWDRNIW
                     TQNGDVINRYDYALPSFGGGLNFRLSESFNLVVQETFMLSSEDDVDRQVNQNNDGFLF
                     HTVGLTFNLGKNKDSDDDGVSDKKDNCPNTPKGVKVDASGCPVDTDNDGVADYIDDCP
                     TAAGPQHLKGCPDRDGDGISDKDDLCPDAKGLVNLKGCPDEDGDGVADNNDKCPETKK
                     GYKVDANGCPFDNDGDGLVNEEDMCPDVPGVVALKGCPDSDGDGVADYEDRCPTIKGT
                     IANKGCPEIAKEDVKKITQIASSIYFESNSDKLKPASLIQLDALVGILKKYDAANLSI
                     EGHTDNKGDDAYNLNLSQKRCDAVKSYLMSKGIFESRLTATGFGETKPIAGNDTPEGR
                     AKNRRVELKTSY"
     gene            complement(1394..>2314)
                     /locus_tag="PK134_07800"
     CDS             complement(1394..>2314)
                     /locus_tag="PK134_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38790.1"
                     /translation="IFYFLSASPMLIPLFNRQFFAESAAPDKLFYYVLYVFRNPHHYL
                     PSFFPIADYIKSGILFLISGLLFFRFPTKYNRQIMVIISGIVGGMIFYYLIFDVGGLM
                     VVGKLQWFKTSVWLTIFCSFYIAHFIAKQNLIQNILNYKWVSACVIALSIFIFYLITH
                     ASLLPLEKLKSRYAVGQFKKTDLTLMHEWIRENTTDTTKILSFPDDDSFLCEAQRPTP
                     VAWKAIIHEPWFLKTWYYNFTTDYNLSRKIEWKNINQREKASSIFLGIQDKPTNASYS
                     MILFNANKLPEQHKGKIIHRQGEYTLWQWN"
BASE COUNT          772 a          360 c          475 g          707 t
ORIGIN      
        1 ttaggattct atggcggagc aactcaatat aatggtgata tgggacaagg tttttataaa
       61 accaatcagc cattttatgg tttcggtgcc atttctgtag ggtattatct cggaaaatat
      121 gccgatttac aaataatggg aactacagga gaagctggat ttattgaaga ccaggtaaat
      181 cgtttcagaa taaaaatgac gacaggaagc ttgcatttgc gttttcattt cacaaaacct
      241 gaagctgcca tcagaccttt cttatttgcc ggagcaggaa taatagtgtg ggacagaaat
      301 atttggactc agaatggaga tgttatcaat cgctatgatt atgcattgcc ttcttttggt
      361 ggcggtttaa atttcagact ttctgaatcg tttaatcttg ttgttcagga aacatttatg
      421 ctttcatcag aagatgatgt ggacagacaa gtaaatcaga acaatgatgg attcttattt
      481 catacagtag gtttaacttt taatcttggc aaaaacaagg attctgatga tgatggtgtt
      541 tcagataaaa aagataattg tccgaataca cctaagggag taaaggtaga tgcatcaggt
      601 tgtcctgttg atactgataa tgatggcgtg gccgattata ttgatgattg tcctacagca
      661 gcgggtcctc agcacctgaa aggctgtcct gacagagatg gtgatggcat tagtgataaa
      721 gatgatttgt gtcctgatgc aaaaggtttg gttaatctga aaggctgccc tgacgaagat
      781 ggtgatggcg tagcagacaa caacgacaag tgccctgaaa caaagaaagg atataaagtt
      841 gatgcaaacg gatgcccttt tgataatgat ggagatgggt tagtaaacga agaagatatg
      901 tgtccagatg ttcccggagt tgttgcgttg aaaggctgtc ccgacagtga tggcgatggt
      961 gttgcagact acgaagacag atgccctacc ataaaaggta ctatcgccaa taaaggttgt
     1021 cctgaaattg caaaggaaga tgtgaaaaaa ataacacaaa tagcgtcttc aatttatttt
     1081 gaaagtaaca gcgataaact caagcctgct tcactcattc agcttgatgc attagttgga
     1141 atattgaaaa agtatgatgc agcaaatctg tcaatagaag ggcatactga taataagggt
     1201 gatgatgctt ataatctaaa tctttcgcaa aaacgttgtg atgccgttaa gagctatctg
     1261 atgagcaaag gtatttttga atcaagatta acagctaccg gttttggtga aactaagcca
     1321 attgccggaa acgacactcc tgaaggaaga gctaaaaaca gaagagtaga gctcaaaaca
     1381 tcttattgat tttttagttc cattgccata gggtatattc tccttgccgg tgaattattt
     1441 tacccttgtg ttgttctggt agtttattag cattaaataa aatcattgag taacttgcat
     1501 tagttggttt atcttgtatg cctaaaaaaa tactacttgc cttttctctt tgattgatat
     1561 ttttccattc aatttttctt gacagattat aatcagttgt gaaattataa taccatgttt
     1621 tcagaaacca tggttcatgt ataatggctt tccatgccac aggtgtaggt ctttgtgctt
     1681 cgcataaaaa tgaatcatca tcaggaaaac tcaaaatctt tgtagtgtca gttgtgtttt
     1741 ctctgatcca ttcatgcatc agtgttaaat cagtcttttt aaactgacca actgcatagc
     1801 gtgattttag tttttctaat ggtaataatg aggcatgtgt gattaaataa aagataaaaa
     1861 ttgataaagc aattacacaa gcagataccc atttataatt taagatattt tgtatcaaat
     1921 tttgctttgc aatgaaatgt gctatataaa aactacagaa aatagtaagc caaaccgaag
     1981 ttttaaacca ttgtagttta ccaacaacca ttaatccacc gacatcaaaa attaaatagt
     2041 aaaaaatcat tccgccaaca atgcctgaaa ttattaccat tatttgtctg ttgtattttg
     2101 tcgggaatct gaaaaataac aagcctgata tcagaaataa tataccactt ttaatataat
     2161 ctgcaatggg gaaaaaggaa ggtagataat gatgtgggtt tctgaaaaca tataaaacat
     2221 aatagaaaag cttgtcaggt gctgcacttt ctgcaaaaaa ctgacgattg aataaaggta
     2281 tcagcatagg agaggccgac aaaaagtaga aaat
//
LOCUS       DAORQT010000260         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9580_length_2312_cov_5.932211, whole genome shotgun sequence.
ACCESSION   DAORQT010000260 DAORQT010000000
VERSION     DAORQT010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9580_length_2312_cov_5.932211"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="PK134_07805"
     CDS             <1..2204
                     /locus_tag="PK134_07805"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38791.1"
                     /translation="SNACGSSSAQVNIVVNPTPYSQVTLTYPNGHCAGSYANACASDT
                     SNGAYAPYTYLWDNGQTTSCINQQILANPLPLQGPSVTITNSLGCTSTNIAVWNYPFG
                     PSADSVQNPQTICQGGSYSINGNNYSTAGTYYDNFINVSGCDSIVITQLTVTPSLSIN
                     VSGNNTICEGQSTTLTATGASTYSWSPATGLNTSTGATVDATPAATTIYVVTGTSNGC
                     SNTATFTVNVNPNPVIQTSGNVDVCSGQFVTLSASGASNFTWSPSTALNTTTGASVVA
                     NPISTITYTVVGTDNGCSASSSITVNIIAQPVVNVSASTTSICNGQSATLTASGADTY
                     TWSPSTGLNTTSGSTVLATPYTTMTYTVTGFVAGGCFSTNTITINANTGTAPTIPGLI
                     AGNKKPCPNVNENYSVSPVANAVSYTWTVPAGVTIISGQGTNNIHLNFASNFTSGNIS
                     VVANGACGVSASRSSSLSLNIPATPSSITGPVTGLCDAAGTFTSATVSGATSYTWSVP
                     AGVNIASGQGTKTASIYVGSSFTSGNVCVTADNTCMSSKPRCVAIKKTPSVPSTLTGP
                     TTVCAGQQNVSYSTNTVFGATNYQWIVPSGSVITSGQGTPNITVDFGTKTGSVGVNAY
                     NACGQAGRRAVLVSFNCRLGEMSSSTSVIEISPNPTSTGMVNLTVNSEVNGIALIKMT
                     DILGKSVLIKNIANVIGTNNHKLDLSKFNKGIYLLTVDTGSKKQTLKVVIQ"
BASE COUNT          756 a          454 c          447 g          655 t
ORIGIN      
        1 gaagcaatgc ttgcggaagt tcatctgctc aggttaacat tgttgtaaat ccgacaccct
       61 attcacaagt aacattaact tatccaaatg gacattgtgc aggcagctat gctaacgctt
      121 gtgcttcaga cacatcaaac ggagcctatg caccatacac ctacctatgg gataatggtc
      181 aaacaacttc ttgtataaat caacagatac ttgcaaatcc attacccttg caaggcccga
      241 gtgttactat tacaaatagt ttaggatgta catcaacgaa tattgcagtt tggaattatc
      301 cttttggccc ttcggccgat tcagttcaga atccacaaac aatttgtcag ggaggctctt
      361 actctattaa tggaaataat tattctaccg caggaacata ctacgataac tttatcaatg
      421 tatccggttg tgatagtata gtaattacac aacttacagt tacaccttcg cttagtatta
      481 atgttagtgg aaacaatacc atctgcgaag gacagtcaac aacattaaca gcaacaggtg
      541 catcaactta ttcatggagt cctgcaacag gattaaatac ttcaacagga gcaacagtag
      601 atgctacacc tgctgcaaca actatctatg ttgttacagg cacatcaaac ggctgctcaa
      661 atacagcaac ttttacagta aatgtaaatc caaatcctgt tattcaaaca agtggaaatg
      721 tagatgtttg cagtggacag tttgtgactt tatcagccag tggtgcaagc aactttacat
      781 ggtcgccttc aacagcatta aatactacaa caggtgctag cgttgttgct aacccaatat
      841 caacgattac ttacacagtt gtaggtacag ataatggatg ttctgcttct tcttcaataa
      901 ctgtgaatat tattgctcag cctgtcgtaa atgtttcagc aagtacaaca tctatatgta
      961 atggtcagtc tgctacgctc actgcatcag gagcagatac atacacatgg agcccatcaa
     1021 caggcttaaa tactacatca ggttcaacag tacttgcaac accatacaca acaatgacct
     1081 acacagttac gggttttgtt gccggaggtt gctttagtac aaataccatt accattaatg
     1141 caaacacagg cactgcaccg actatacctg gtttaatagc aggaaataaa aagccttgcc
     1201 cgaatgtgaa tgaaaattat tctgtaagtc ctgtagctaa tgccgtttca tatacatgga
     1261 ctgttcctgc aggggtgact attatttcag ggcaaggaac taataatatt catttaaact
     1321 ttgcatcaaa tttcacaagt ggtaatatat cagttgttgc aaatggtgct tgcggtgtga
     1381 gtgcttcacg ctcatcttcg ctgtcattga atattcctgc cacaccatca tctatcacag
     1441 gtccggttac tggattatgt gatgcagcag gcacatttac aagtgcaact gttagtgggg
     1501 ccacaagtta tacatggtcg gttccggccg gagtaaatat tgcaagtggt cagggaacta
     1561 agactgcttc aatttatgta ggcagcagct ttacgagtgg caatgtatgt gtgacagccg
     1621 acaacacatg tatgagtagc aagcctcgtt gtgtagcaat taagaaaaca ccatcagtgc
     1681 catcaacttt aaccggacca acaacagttt gtgcaggaca acaaaatgtg tcatatagta
     1741 caaatactgt atttggtgca accaactatc aatggatagt tccatcagga tcagtaatta
     1801 catcaggtca gggaactcct aatattacag ttgattttgg aacaaagacg ggaagtgttg
     1861 gtgtcaatgc atacaatgca tgtggacaag caggaagaag agcagtactt gtttcattca
     1921 attgccgctt aggcgagatg agtagtagta cttcagttat tgaaatttcg ccaaatccaa
     1981 cttcaacagg aatggttaac ctgacagtta attcagaagt taatggtata gctttaatta
     2041 aaatgactga tattttagga aagtcagttt taataaagaa cattgcaaat gtgataggta
     2101 caaacaatca taaattagac ttgtcgaaat tcaacaaggg aatttatttg ttgactgttg
     2161 ataccggtag taaaaaacaa acattaaaag ttgtaataca ataattatat ctaaaaccgg
     2221 aataatgccc tcctacggga gggtttttat ttttaaagaa agagaagaac agcaacttcg
     2281 aaaacatttt tgaaaaagtt tccagaacaa tg
//
LOCUS       DAORQT010000261         2310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9594_length_2310_cov_4.725055, whole genome shotgun sequence.
ACCESSION   DAORQT010000261 DAORQT010000000
VERSION     DAORQT010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9594_length_2310_cov_4.725055"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PK134_07810"
     CDS             <1..607
                     /locus_tag="PK134_07810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRB38792.1"
                     /translation="RTTLSGPSNLSDFMQKGTEQVAAGYVIYGSSTMMVYTTGKGVNG
                     FTLDPSIGEFCLSHPDMKIPKQGFIYSVNEGNYAHFPEGVKKFIKFCQEEDEKTKRPY
                     SSRYIGSLVADFHRNLLKGGIYVYPATAKSPKGKLRLLYECNPLAFIVEQAGGKASDG
                     FNRILDIQPTSLHQRTPLFIGSPDMVTKAEELMMRYSQMGA"
     gene            682..1263
                     /locus_tag="PK134_07815"
     CDS             682..1263
                     /locus_tag="PK134_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB38793.1"
                     /translation="MSDDTNQTSYPEENKLGITDKRFINEIESKGVATAEYFIFELDT
                     DIIISSQLICEIHRIAFSELYEWAGKWRTVSVYVGNLIPPEPGQVVQLMYQYIDNLNF
                     KLINSKHTQDHLDCLIFAHYEFVRIHPFNNGNGRTARILMNLVALKLGYKPLELYHRQ
                     GDNRNIYINALRLADKGNFELLLNLIRKELTPF"
     gene            1580..>2310
                     /locus_tag="PK134_07820"
     CDS             1580..>2310
                     /locus_tag="PK134_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRB38794.1"
                     /translation="MREIQFREALREALSEEMRRDPNVFLMGEEVAQYNGAYKVSQGM
                     LDEFGAKRVIDTPIAELGFAGIGVGAAMNGLRPVIEFMTFNFSLVAIDQVINAAAKMK
                     SMSGGQYGVPIVFRGPTASAGMLSSQHSQAFESWYANCPGLKVIVPSNPKDAKGLLKA
                     AIRDDDPVIFMESEQMYGDKGEVPEGEYLMPIGVADIKRPGNDVTIVSFGKIIKTAYK
                     AAEELAKENIDVEIIDLRTVRPIDYA"
BASE COUNT          742 a          394 c          457 g          717 t
ORIGIN      
        1 aagaacaaca ctttcaggcc ccagcaacct ttctgatttt atgcaaaaag gtactgaaca
       61 ggtggctgcc ggttatgtta tttacggctc atcaaccatg atggtttata caacaggcaa
      121 aggtgttaac ggatttacat tagatccttc tatcggtgaa ttctgtcttt cgcatcccga
      181 tatgaaaata ccaaaacaag gatttattta ttctgtaaac gaaggtaact atgcacattt
      241 tcctgagggc gtaaagaagt ttataaaatt ctgtcaggag gaagatgaaa aaacaaaacg
      301 accatattca tcaagatata taggatcgtt agttgccgat ttccaccgca atcttttaaa
      361 aggtggcatt tatgtttatc cagccacagc aaaatcacca aaaggaaaac taagattgct
      421 ttatgagtgt aatccgttgg catttattgt agagcaagcc ggtggcaaag cctctgacgg
      481 atttaaccgc atactcgata ttcaaccaac atctttacat cagcgaacac cattatttat
      541 tggctctcct gatatggtta caaaagcaga ggagttgatg atgcgctatt cgcaaatggg
      601 tgcatagaat tgtttgaagt gtttgttgtt atctttaatt taacatctaa ctaaaagaat
      661 gaatgattaa ttgaagtaag catgagtgat gataccaatc aaacatcata tcctgaagaa
      721 aataaactag gaataacaga caaaagattc attaatgaga ttgagtcgaa aggtgtagca
      781 actgctgaat attttatttt cgaactcgac acggatatta ttatttcttc tcaattaata
      841 tgcgaaattc acaggattgc attttcagaa ttatatgaat gggccggtaa atggagaaca
      901 gtatcagtat atgtcggtaa tctgataccg cctgagccag gtcaggttgt tcaattaatg
      961 tatcagtaca ttgataattt aaattttaaa ctaatcaatt ccaaacacac tcaggatcat
     1021 ctcgattgtt taatatttgc tcattatgag tttgtcagaa ttcatccttt caataatggt
     1081 aacgggcgta cggcaaggat attgatgaat cttgttgcat tgaaattggg gtacaaacct
     1141 cttgaacttt atcaccggca aggcgataat cgtaatattt atatcaacgc attaagattg
     1201 gctgataaag gaaattttga gctattattg aatttgatca gaaaagaatt gacccccttt
     1261 tgattccttt tcattcagaa gtgatttaac aatctcctga atttttttca tcggaccttc
     1321 ttgattttac aaagccattt tggcaaaaac agattttgac aagagttctg ctaaaaattc
     1381 tttttctttt aattgtggat atttttcgta aaatttcatt tgcattacaa agataaataa
     1441 aactttttcg caaaacaatt taaatatatt caagttgctt ttagaataat tggttagaca
     1501 aataacaatt catgatattc catcactgtt tttgaacact gtattgattt tatgtacatt
     1561 tgtggcatag aacaatatta tgcgcgaaat acaattcaga gaagccctgc gagaagcctt
     1621 aagcgaagaa atgcgcaggg accccaatgt ttttttaatg ggcgaggagg ttgcacaata
     1681 caacggtgca tataaagttt cgcaaggcat gttggatgag tttggtgcca aacgtgttat
     1741 tgacacacct attgcagaat tgggttttgc aggaataggt gttggtgctg ccatgaatgg
     1801 tttgcgtcct gttatagagt ttatgacctt taatttttca ttggttgcca ttgatcaggt
     1861 tattaatgca gcagctaaaa tgaaaagtat gagtggcgga caatatggag tacctattgt
     1921 ttttcgcggg cctactgcat cggcaggtat gctcagttca caacactcac aggcatttga
     1981 aagttggtat gccaactgcc ccggactaaa agttattgtt ccctcaaacc ctaaagatgc
     2041 caaaggactt cttaaagccg caatacgtga tgacgatcct gtaattttta tggaaagcga
     2101 acaaatgtat ggcgacaaag gcgaagtgcc tgaaggtgaa tacctgatgc ctattggcgt
     2161 tgcagacatc aaacgtcctg gcaatgatgt taccattgta tcgttcggaa aaattataaa
     2221 gactgcatat aaggcagcag aagagttagc caaagaaaat atagacgttg aaattattga
     2281 cctgcgtacg gtgcgtccta ttgactatgc
//
LOCUS       DAORQT010000262         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9729_length_2288_cov_5.171518, whole genome shotgun sequence.
ACCESSION   DAORQT010000262 DAORQT010000000
VERSION     DAORQT010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9729_length_2288_cov_5.171518"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PK134_07825"
     CDS             <1..607
                     /locus_tag="PK134_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HRB38795.1"
                     /translation="GKVDIGLIPVAVLPLVKNAQIITSFCIASNGNVNSVLLLSQVPL
                     NEIKQIWLDYHSRTSVMLAQLLAKEFWKIDVSWLPSEKGFENKIEGQTAGVVIGDRAL
                     ALKSNFKYVYDLATEWKKHTGLPFVFACWVSNKSLPEDFIVAFSHALNQGVAMIPELS
                     KQLQPDYLFDVKSYLTENIDFRLDEEKEQALKLFLERIELK"
     gene            complement(660..1181)
                     /locus_tag="PK134_07830"
     CDS             complement(660..1181)
                     /locus_tag="PK134_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB38796.1"
                     /translation="MKNSDIIFKDSVKDHYRSLRGFALKLTQNTEDANDLIQETMLKA
                     LNNEDKFEKGTNLSGWLYTIMRNIFINNYRRMVKSQTFLDSTENQFFLNNIKNATRNR
                     GEANLVMKEINSAINDLNDNLKVPFMMSYRGYKYEEIASQLSIPLGTVKIRIHTARQK
                     LKEKLNVYNSLSF"
     assembly_gap    1425..1448
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1449..2082)
                     /locus_tag="PK134_07835"
     CDS             complement(<1449..2082)
                     /locus_tag="PK134_07835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002102764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB38797.1"
                     /translation="MKISIVTITYNSAATIEDTIKSVVVQDYPDMEYLLIDGKSKDST
                     LQIAEKYKDKITKIVSEKDGGLYYGLNKGLKMAAGDVIGAIHSDDLYAHPQVVSNVMK
                     LFNADPELEGVYADLIFVNREDTNKVMRVWKAGEYKPDSFEKGWMPPHPTFFVRKSVY
                     EKYGYINTDLKLSADYELMLRLIHKHKIKIAYLPETIVKMRMGGVSNVSFF"
BASE COUNT          678 a          400 c          385 g          801 t
ORIGIN      
        1 tggcaaagta gatatcggat tgatacctgt ggcggttctg ccattagtaa aaaatgcaca
       61 aatcattact tctttttgta ttgcctcaaa tggcaatgtc aactctgtgt tgttgctaag
      121 tcaggttcct ttaaatgaaa taaaacaaat ctggcttgat tatcatagca gaacttcggt
      181 aatgctggca cagttacttg caaaagagtt ttggaaaatt gatgtcagtt ggcttccttc
      241 cgaaaaagga tttgaaaata aaatagaagg tcaaactgcc ggagttgtta taggcgacag
      301 agcattggca ttgaagtcga atttcaagta tgtgtatgac cttgccacag aatggaaaaa
      361 acatacaggc ttaccttttg tctttgcttg ttgggtaagt aataaatcat tacctgaaga
      421 ttttatagtt gcattttctc atgcactcaa tcaaggagta gcaatgattc ctgaattaag
      481 taagcaatta caacctgatt atctttttga tgtaaaaagt tacttaactg aaaacattga
      541 cttcagacta gatgaagaaa aagaacaagc attgaaattg ttcttggaac ggattgagtt
      601 aaagtaaatc aaaacgcaat tttaaaatgc ctttacgttg taatctgaac cctactacct
      661 caaaacgaca atgaattgta aacgttaagt ttctctttca gtttttgtct tgcagtgtgt
      721 attctgattt ttactgtgcc taatggaatg gataattgtg atgctatctc ttcgtatttg
      781 tatccacggt agctcatcat gaatggaact ttaaggttgt cattcaaatc gtttattgca
      841 ctgttgattt ccttcatcac caagttagcc tctcccctgt ttctggtcgc attcttaatg
      901 ttattgagaa aaaactgatt ctctgtgcta tctaaaaatg tctgactttt taccattctg
      961 cgatagttgt taatgaaaat atttcgcatt atggtgtaaa gccatccgct aaggttggtg
     1021 cctttttcaa atttatcttc attgtttaat gcctttaaca ttgtttcctg tatcaggtcg
     1081 ttggcatctt cagtattttg agtgagtttt aatgcaaaac ctcttaaaga acgatagtgg
     1141 tcttttactg agtctttaaa tatgatatct gagtttttca tttgtttaac ctttttaaat
     1201 taaagctcaa caaacttacg ataccaacat gaattatgta ttaatgttta acttaaataa
     1261 tgttaaagtt aaacaatatt gcaagatgca gacaatcaaa caaatggctt agtagaagaa
     1321 agtattgtat gcctttttaa aatactgact gattttccga agaggcttac gtaatgttgt
     1381 gaataatccg ggcttcaggt tattcatttt ccaggcaagt ttatnnnnnn nnnnnnnnnn
     1441 nnnnnnnnca aaaaagctga cattactaac gccccccatt ctcattttaa caatagtttc
     1501 ggggagataa gcaatcttta ttttatgttt atgaattagc ctgagcatta attcataatc
     1561 ggcagaaagt tttaaatcgg tattgatgta gccatacttt tcataaacag atttgcgcac
     1621 aaaaaatgta gggtgaggcg gcatccatcc tttttcaaat gaatcgggtt tatactctcc
     1681 tgccttccaa acacgcatta ctttattggt atcttcacgg tttacaaaaa tcaaatcggc
     1741 ataaacacct tccagctcgg ggtcggcatt aaacagtttc atgacattgg aaacaacttg
     1801 cggatgtgca tataaatcgt cagagtgaat tgctccaatt acatcacctg ctgccatttt
     1861 taaaccttta ttgagtccat agtaaagtcc tccgtctttt tcagaaacta ttttggttat
     1921 tttatccttg tacttttctg caatctgaag tgtgctgtcc tttgatttac catcaatcag
     1981 caaatactcc atatcaggat agtcctgcac gacaacagat tttatggtgt cttcaatagt
     2041 tgctgcacta ttataggtta tcgttacaat cgaaatcttc atattcatta tgttccttag
     2101 tgtcttttac ccattctaca aaaataggtt gcaaaatcat tctgcaaaca attttttatt
     2161 ctgatttacc gtatctgagt tgtttaatac ttctttagca agttccattg ctccttgttg
     2221 ccacaactta taagttttct gatccattga agctgctttt actatactat caataaactg
     2281 ctgttgat
//
LOCUS       DAORQT010000263         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9790_length_2278_cov_4.553306, whole genome shotgun sequence.
ACCESSION   DAORQT010000263 DAORQT010000000
VERSION     DAORQT010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9790_length_2278_cov_4.553306"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="PK134_07840"
     CDS             <1..1757
                     /locus_tag="PK134_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HRB38798.1"
                     /translation="RQFGTNNLPDCSNMCHESSGVALTESLGIGKGSVTLDDFYNAEV
                     IIILGQNPGTNHPRMLTALQKAKENGCTIISVNPLPETGLIAFNDPQSIKGALGIKAR
                     LTNIFLQVKINGDMALLQAIEKLLLIEEEKNPGSVFDHEFINSKTNGSNEFIQHLKNL
                     DLNNLIAASGISKEQILEVANIFKTKSKIIACWAMGLTQHQNAVDTIKEVVNLLLLKG
                     SIGKPGAGTCPVRGHSNVQGDRTMGIYEKPSPSFLDAIQKTYSFNPPREHGYDVVDCI
                     HAMHKGKVNVFMAMGGNFLSATPDTTYTAEALRKCKLTIQVSTKLNRSHLITGEEALI
                     LPVLGRSDKDVLNGEEQFITTENSMGVIQMSKGNLTPVSEQLLSEPALVAKLAVEYFG
                     NQTQVPWKKYLEHNDHIRDDIAKTISGFDNYNTRVRKLGGFYLPNSAREQKFTVPSGK
                     AEFNIGNVKEFNLQPNEYVMMTIRSHDQFNTTIYGLNDRYRGVINERRVIFMNEKDIA
                     AGGFTKGEVVDLYNFDNNIERVAHRFIIIPFSIPEKCTATYFPETNVLVPIDSTAEKS
                     NTPTSKSVIIEVRRSATS"
     gene            1926..>2278
                     /locus_tag="PK134_07845"
     CDS             1926..>2278
                     /locus_tag="PK134_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB38799.1"
                     /translation="MTKNDADEIFRLRSDKKITEYLDRDPPKSIEEIYEWVIKVDAEI
                     DENRGISWGMYLKGEPKLIGSIGLWRTVPEHHYAEIGYSLLNEYQRKGLMFEAMIAAL
                     KYGFNEMQLHRVEAN"
BASE COUNT          803 a          405 c          455 g          615 t
ORIGIN      
        1 taagacaatt cggcacaaat aacttaccgg attgcagcaa catgtgccac gaatcaagcg
       61 gtgttgcttt aacagaaagt ctaggcattg gaaaagggtc tgttacatta gacgattttt
      121 ataatgcaga ggttatcatc attcttggac aaaatccggg cacgaatcat cccagaatgt
      181 taaccgcttt acaaaaagca aaagagaatg gatgcactat aatttccgta aatccattgc
      241 cggagaccgg acttattgct ttcaatgatc cacaaagtat aaaaggtgca ttaggtatta
      301 aggcccgact tacaaatatt tttcttcagg taaagatcaa tggcgacatg gctttactgc
      361 aggctattga aaaattatta cttatagaag aagaaaagaa tcccggaagt gtttttgatc
      421 atgaatttat taatagtaaa acaaatggct caaatgaatt cattcaacac ttaaagaatt
      481 tagatttgaa taatctaatc gctgcttcag gaattagcaa agaacagatt ttagaagttg
      541 ccaatatatt caaaacaaaa agtaagatca ttgcatgttg ggcaatggga cttacacaac
      601 atcagaatgc agtagatact attaaagagg ttgtcaactt attgctctta aaaggtagta
      661 ttggaaaacc gggagcagga acctgtcctg tccgtggaca tagcaatgta caaggcgatc
      721 gaacgatggg catttatgaa aaaccatcac cttcattttt agacgcaatt caaaaaacgt
      781 attcattcaa tccaccacga gaacacggat atgatgtcgt tgattgcata catgccatgc
      841 acaaagggaa agtaaatgta tttatggcaa tgggaggaaa ctttttatca gcaacaccgg
      901 atacaaccta tacagctgaa gcattgcgaa aatgtaagtt aactatacaa gtatcgacta
      961 aattgaatcg gagtcatctc atcaccggcg aagaagcttt gatccttcca gttcttggaa
     1021 gaagtgataa agatgttttg aatggtgaag agcaatttat cacaacagag aattctatgg
     1081 gagtaattca aatgtcgaaa ggcaacctta ctcctgtttc agaacaattg ttgagcgaac
     1141 ctgcactagt tgcaaaactt gcagtagaat acttcggaaa tcaaacacag gttccttgga
     1201 aaaaatatct agagcacaac gatcatattc gtgatgacat agccaaaaca atttccggat
     1261 ttgacaatta taatacaaga gtcaggaaac tcggcggatt ctatttaccg aattcagcac
     1321 gagagcaaaa attcactgtt ccatccggaa aagcagaatt caatattggt aatgtaaagg
     1381 agttcaattt acaacccaat gaatatgtaa tgatgaccat tcgaagtcac gatcagttca
     1441 atactaccat ttacggactg aatgatcgtt atagaggcgt aatcaatgaa cgtcgtgtga
     1501 tattcatgaa tgaaaaagat attgcagccg gtggatttac aaaaggtgaa gttgttgacc
     1561 tgtataactt cgacaataac attgaaagag tagctcatcg atttataatt attcctttca
     1621 gcattcctga aaaatgtact gcaacttatt ttccggaaac aaatgtttta gtaccaattg
     1681 acagtactgc agagaaatcg aatacaccga cttcgaagtc ggtcattatt gaagttcgta
     1741 ggtcagcgac ttcttgatag accaggtcaa aggtcaaggg tcaaaggtca aatgatgatc
     1801 gtacacttga gccttgctta acctttgact tctgaccttt gaccaaaaaa tccttcatca
     1861 atatgctaac attcaacttc accccttttc ccacaataga aaccgaacgg ctattacttc
     1921 atcgtatgac aaaaaacgat gctgatgaaa tattcaggtt gaggagtgac aaaaagataa
     1981 cagagtatct cgatcgggat cctccgaaat cgattgaaga aatttacgaa tgggtaataa
     2041 aagttgatgc ggagatcgac gagaatcgtg gcatatcgtg gggaatgtat ttaaaaggag
     2101 agccaaaact gatcggcagt atcggtcttt ggagaacagt tcctgagcat cactacgctg
     2161 aaattggtta ttcactgtta aatgaatatc aaagaaaggg attgatgttc gaagcaatga
     2221 ttgcagcact taaatatggt ttcaatgaaa tgcaattaca cagagttgaa gcaaacat
//
LOCUS       DAORQT010000264         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9815_length_2275_cov_6.038739, whole genome shotgun sequence.
ACCESSION   DAORQT010000264 DAORQT010000000
VERSION     DAORQT010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_2275_cov_6.038739"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..741
                     /gene="apaG"
                     /locus_tag="PK134_07850"
     CDS             355..741
                     /gene="apaG"
                     /locus_tag="PK134_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRB38800.1"
                     /translation="MSTEVTAGIKVTVETFFQPTHSNPVQHHYVFAYRITISNESEES
                     IKLKRRHWYIIDSDNARREVEGEGVVGEQPVIEPGQLYRYVSGCNLNTEIGKMYGTYL
                     MERLSDNKMFYVKIPEFRMVVPSKMN"
     gene            832..1479
                     /gene="rpe"
                     /locus_tag="PK134_07855"
     CDS             832..1479
                     /gene="rpe"
                     /locus_tag="PK134_07855"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRB38801.1"
                     /translation="MHLIAPSILSADFSQLSKDVEMINNSVADWFHVDVMDGVFVPNI
                     SFGFPVIKAIKKHAKKPLDVHLMIIKPERYVEQFREAGADVLTVHIEASVHLHRTIQQ
                     IKKEGMKAGIAINPHTPVASLSDIIFEADLICMMSVNPGFGGQKFIENTYRKIQELKE
                     LIAIHNSKTLIEIDGGVDLNNYKKLIAAGADVLVAGNTVFSSSDPKATIEVLKKG"
     gene            complement(1483..>2275)
                     /locus_tag="PK134_07860"
     CDS             complement(1483..>2275)
                     /locus_tag="PK134_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRB38802.1"
                     /translation="YNGIMAYGLKYGLFNLKAAHQSFGIEELHKELDSIKLPNIKIVI
                     TGGGRVANGAIETMGMLNIRKVTPFEFLNYSFREPVYVQLHSENYYERKDGSDFLKAD
                     FYHYPEKYNCTFCDPGSYSSFTDLLVHCAYWNPNAAVLFTKEDMRSPAFRISVIADVT
                     CDIDGSIPSTHRATTIDDKFYGYNPMNEKEEEAFSTDTITVMSIDNLPCELPRDASEG
                     FGKHLIERVIPLLIGEDKGNVIYRATIARNGELTPRFAYLTDYIS"
BASE COUNT          762 a          435 c          407 g          671 t
ORIGIN      
        1 gtatcaagct gaagaagatt ttaagaaaga attacttcgt caacttaaag acagaagaga
       61 tgcacgaggt aattaatcat ttataatgtt tagtaataga ttaagcgggc aatgctcgct
      121 tttttatttg tataaagaat cattttaccc aaaaaactaa ctatgattaa ttattatcct
      181 tcatttttaa ctcaaattct ctgcttttca ccaaaaacaa atctacattt ttaatgccta
      241 tacagataaa atcaaccttc gccctgtaaa aaagcacatt taccaaaaca acctaacatt
      301 gcatacatgc atgtttaatt ttacatccgt tatacacaac aacacatcat gtatatgtca
      361 acagaggtaa cagcgggaat aaaggtaacg gtagaaacat tctttcaacc tacccattcc
      421 aaccctgttc agcatcatta tgtattcgca tatcggatta ccatttctaa cgaaagcgaa
      481 gaaagcatta aactaaaacg cagacattgg tatatcattg acagcgacaa tgccagacgc
      541 gaagttgaag gcgaaggcgt tgttggagaa caacctgtta ttgaaccggg tcagctttac
      601 cgatatgttt cgggatgcaa cctgaatact gaaataggaa aaatgtatgg cacctatcta
      661 atggaacgcc ttagcgataa taaaatgttt tatgttaaga tacctgagtt tagaatggtt
      721 gtgccttcaa aaatgaatta gtcccaatag ttatttgatt tgaattttta aaaagagagc
      781 cgccaggctc tttttttatg gataaaagtt agttttgcat aaagtttcac catgcacctt
      841 atagcacctt ccattctttc tgccgatttt tcacaactga gtaaagatgt ggaaatgatc
      901 aacaacagtg ttgcagattg gtttcatgtt gatgttatgg atggtgtttt cgtgccaaac
      961 atttcttttg gatttccggt aataaaagcc ataaagaaac atgcaaaaaa gcctttggat
     1021 gttcatctta tgattataaa acccgagaga tatgttgaac agtttcgtga agcaggtgct
     1081 gatgttctta ccgttcatat agaggcatct gttcatctgc accgaacaat acagcagata
     1141 aaaaaagaag gtatgaaagc aggtattgcc ataaatccac acacgcctgt ggcatcattg
     1201 agcgatatta ttttcgaagc cgacttaatt tgtatgatga gcgtgaatcc gggttttggc
     1261 ggtcagaaat ttatagaaaa cacgtacaga aaaattcagg agttgaaaga gctaattgct
     1321 attcacaact caaaaacact tatagaaatt gatggtggtg ttgacctgaa taactacaaa
     1381 aaacttattg ctgccggtgc cgatgttctt gttgcaggaa ataccgtttt tagctcttct
     1441 gacccaaagg ccacaattga agtacttaaa aaaggataaa catcaactga tgtaatccgt
     1501 aagatatgca aatcgtggag tcaattcacc attgcgggct atggtagcac ggtatatcac
     1561 attgcctttg tcctcaccta tcaaaagagg aataacacgt tcaattaaat gcttaccaaa
     1621 accttcagaa gcatcacgcg gtaattcgca aggaaggtta tcaatagaca tgacagtaat
     1681 ggtatccgta ctgaatgctt cctcctcttt ttcattcatt ggattataac cataaaattt
     1741 atcgtcaata gttgttgcgc gatgcgttga aggaatacta ccatcaatat cgcaagtgac
     1801 atctgcaatg acactgatac ggaatgcagg gcttcgcata tcttctttcg taaacagcac
     1861 agcagcatta ggattccaat aggcacaatg caccaacaag tcggtgaacg atgaatatga
     1921 tcccgggtca cagaaagtgc aattatattt ttcagggtag tgataaaagt cggccttcaa
     1981 aaaatctgac ccgtctttgc gttcgtaata attttcggaa tgtagctgca cataaacagg
     2041 ctcacggaat gaataattca gaaattcaaa tggagtaact ttcctgatat ttaacattcc
     2101 cattgtttca atagcaccat tagcaacacg accacctcct gtaatcacta ttttaatatt
     2161 cggaagttta atgctgtcta attccttatg caactcttca ataccaaaac tctgatgcgc
     2221 agctttcaag ttaaacaatc catactttaa accataagcc atgataccat tatat
//
LOCUS       DAORQT010000265         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_9870_length_2266_cov_4.935323, whole genome shotgun sequence.
ACCESSION   DAORQT010000265 DAORQT010000000
VERSION     DAORQT010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9870_length_2266_cov_4.935323"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..>2266)
                     /locus_tag="PK134_07865"
     CDS             complement(366..>2266)
                     /locus_tag="PK134_07865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRB38803.1"
                     /translation="LIALPSVEAVEDNGMQLVALDDSMRVRNNINPVQNGMAPLPQGY
                     DGSGVVMGFIDSGIDFSHPDFKHANGSTRVLYIWDHNLTVGNAPMPYNYGTEFTAADI
                     DAGNASAHVDNSNGHGTHVTCMGSGNGLALNDHKGAAPAADIISVCVNWNLSDNDWLT
                     TVADAVNYIYSKAYALNKPCVINISAGTYYGSHDGKDLQAQAISNLISQRNGRSLVCA
                     AGNAGNIPIHVQHLHTAANDTSFTWFNFNPAYGNSIYLEMWADVANFTQMKFAVGADN
                     VTSGYELQTMGNYYTVSQNLGTIHTDTLWGVSGNRIALVQRFASLSNGRYSMIFNIIP
                     DSTQYRFRLSSTQTGKFDLWSFQMTPSNALPTPQQFPEIVKYVSPDYDQTMVSSFSCS
                     DKVITVGEHKNRMTYMDCTNNLFVSTTNYTPGALAPQSSHGPTRDARLKPEICASGGM
                     SIAAGAYNVLPNLPVTSKALGCMHIRDGGTSTASPVVAGIAALILQRYPNASWQDIKN
                     CITQNAMVDSLVTGTIPNNSWGYGRADGFAAMLGCSFLSINDYDSQIGSIYPIPFNQS
                     FQLTAVKQLSAVNIFNPLGQLVYQSSFNIEATHKITINPILPSKGVYFAQLISTEGYK
                     QTIKLIYQ"
BASE COUNT          720 a          422 c          411 g          713 t
ORIGIN      
        1 tgaatcacga tttatcaacc gttcaaacat ttcaagtgtt tcaatattta atatttcgtt
       61 ttcccttttc acagctagtt atttaataat ctatttaagg aatattcatt acttatttca
      121 atataagttc aattattatt gcataatagt ataaatatag aacaatgaaa aacttacata
      181 aaatttaccg gaaaattagc atttaatttt acagttattt aacttgaagt aaaatttaac
      241 aattgatagt tctaaaaatg tattatgtag aattttacct aaagcaaata cttaataaaa
      301 catatctgct tgattagtca attatcttaa gtttctttgg ggttaagttt gatgttttaa
      361 aatgtctatt gatatatcaa ttttatcgtc tgcttataac cttcagtaga aatcaattga
      421 gcaaaataaa cgccttttga aggtaatata gggttaattg ttatcttatg cgttgcttcg
      481 atattgaaag atgattgata aaccaactgt cctaatgggt taaaaatatt tactgctgaa
      541 agttgtttca cagccgttag ctgaaatgac tgattaaaag gaattggata aatacttcca
      601 atttgtgaat cataatcatt gattgaaaga aaactacaac caagcatggc agcaaatcca
      661 tctgcacggc catatcccca gctgttgttt ggtattgtac ctgtaaccaa tgagtcaacc
      721 attgcatttt gtgtgataca gtttttaata tcctgccacg atgcatttgg gtatcgttga
      781 agtatcagtg ctgcaatacc ggcaactaca ggcgatgctg tagaagtacc tccatctctg
      841 atgtgcatgc aacctaaagc cttacttgtg accggaagat tgggtaacac attataagca
      901 cctgctgcaa tagacattcc accgctggca caaatttcag gtttgagacg tgcatcacga
      961 gtagggccat gactgctttg tggcgccaat gctcccggtg tatagtttgt tgttgaaaca
     1021 aaaagattat tggtgcagtc catataagtc atgcggtttt tatgttcacc aacagtaatt
     1081 actttatcac tgcatgaaaa actacttacc atagtctggt cataatcggg agaaacatat
     1141 tttacaattt caggaaattg ttgcggtgtt ggtagtgcat ttgatggtgt catctgaaaa
     1201 ctccataaat caaatttgcc tgtttgcgtt gaagagagtc tgaagcgata ttgagtgctg
     1261 tccggaataa tattaaatat catactatag cgtccattag aaagtgaagc aaaacgctgc
     1321 acaagcgcaa ttcggtttcc tgaaacaccc cacaatgtat ctgtgtgtat tgttcccaaa
     1381 ttttgagaaa cagtataata gttacccatt gtctgtaatt cataaccgga agttacatta
     1441 tctgcaccga ctgcaaattt catctgagta aagtttgcca catcagccca catttctaaa
     1501 taaatagaat taccataggc agggttaaaa ttaaaccatg taaacgatgt atcattagca
     1561 gcagtatgca aatgctgaac gtgaataggt atatttcctg cattaccggc tgcacaaaca
     1621 agtgagcggc cattacgttg ggaaatgaga ttagagatag cctgtgcctg taaatctttt
     1681 ccatcatgag aaccataata agttcccgca ctgatgttga ttacacatgg cttattaagt
     1741 gcataagctt ttgaataaat ataattcaca gcatcggcaa ctgttgttaa ccaatcattg
     1801 tctgaaaggt tccagtttac acaaacagaa attatatcgg cagcaggtgc agcaccctta
     1861 tggtcgttca atgcaagacc attgccggaa cccatacatg taacgtgtgt gccatgtcca
     1921 ttgctgttgt caacatgagc ggaggcattg cctgcatcaa tatctgctgc agtaaattct
     1981 gttccataat tatagggcat tggtgcattt ccaactgtta aattatgatc ccagatatac
     2041 aacacacgtg tacttccgtt agcatgtttg aaatcaggat gagaaaaatc aatacctgaa
     2101 tcaataaatc ccattactac gccactgcca tcgtagcctt gcggcaaagg agccatacca
     2161 ttttgaacag gattgatgtt gttgcgcaca cgcatagagt cgtctaaagc tacaagctgc
     2221 attccgttat cctcaactgc ttctacactt ggaagtgcaa ttaatc
//
LOCUS       DAORQT010000266         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10029_length_2240_cov_6.855835, whole genome shotgun sequence.
ACCESSION   DAORQT010000266 DAORQT010000000
VERSION     DAORQT010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10029_length_2240_cov_6.855835"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /gene="lhgO"
                     /locus_tag="PK134_07870"
     CDS             <1..1145
                     /gene="lhgO"
                     /locus_tag="PK134_07870"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRB38804.1"
                     /translation="RFPHLKIAVLEKEDRLAAHQTGRNSGVIHSGLYYKPGSYKAKNC
                     VEGRRELVAFCKEHQVAHDVCGKIIVATDEKELPHMNRVFNNGLQNGVEGIEKINAQQ
                     IKDIEPFCTGIEGIWVPCTGIVDYVGLTNKLAELIEQKFPGSKVYTGHEVTAFEKEDG
                     NTTVVTNKQKFKTKHLISCTGLFSDRMAKADGVNPNMQIVGFRGDYYDLTEKGMHKVR
                     NLIYPVPNPEFPFLGVHFTRMIQHGVECGPNAVFTFKREGYGKTDFDLKDTLQALAYI
                     GTWKLFFKHWRFGLDEYKRAFSKKLFLERLQRLIPSLEMDDITPGRAGVRAMALDANG
                     NMIDDFKIEYSGNAIHVLNAPSPAATASLAIGTAVMEMAEKHFQIH"
     gene            1301..1675
                     /locus_tag="PK134_07875"
     CDS             1301..1675
                     /locus_tag="PK134_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRB38805.1"
                     /translation="MRTTILRFTFLVSSILLFSFSSHAQESKTDTIKVYGNCNMCKEK
                     IEGSLKEKDGITAKNWDKKTKLLAVTYNPSKITKEQIGQKIADVGYDNQYATATEKVY
                     KKLHSCCQYDRPKQPAGDEKNK"
     gene            1697..>2240
                     /locus_tag="PK134_07880"
     CDS             1697..>2240
                     /locus_tag="PK134_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38806.1"
                     /translation="MKKQLLTLILFAITSLGMAQTATDFTANDCNGNSHNLFTELNSG
                     KVIVLCWVMPCASCTGPSLTTYNVVQSFQASNPNKVFMYTCDDFANTNCTSLNSWANG
                     IGVMQQSWSLRFSNAAINMTHYGSPGMPKIVVLGPNHAVFYNSNNTVNATNLQNAINN
                     ALATTGLNESNLPASSLSVLT"
BASE COUNT          766 a          399 c          425 g          650 t
ORIGIN      
        1 tgcgtttccc acatctgaaa attgcagtgc tcgaaaaaga agaccgcctt gctgcacatc
       61 agacaggaag aaattcaggc gtaatacatt caggacttta ctataagccg ggatcctata
      121 aagcaaaaaa ctgtgttgaa ggcaggcgtg agcttgttgc tttttgtaaa gaacatcagg
      181 tggcacacga tgtatgcgga aaaattattg ttgctactga tgaaaaagaa ctgccacaca
      241 tgaaccgcgt ttttaataat ggtttgcaaa atggtgttga aggaattgaa aaaattaatg
      301 cacaacagat aaaagacatt gaaccatttt gtaccggcat tgaaggcatt tgggtgccct
      361 gcacaggtat tgttgattat gttgggttga ctaataaact ggcagaactt attgaacaaa
      421 aatttccggg tagtaaagtt tataccggcc atgaagttac tgccttcgaa aaagaagatg
      481 gaaacaccac agttgttacc aataaacaaa aattcaaaac caagcatctt atcagttgca
      541 cagggttgtt cagcgacaga atggcaaaag cagatggcgt aaatcctaat atgcagattg
      601 taggtttcag aggtgattac tacgatctga cagaaaaggg aatgcataaa gtgcgcaatc
      661 ttatttatcc tgttcctaat cctgagtttc cttttttagg tgtgcatttt acaagaatga
      721 ttcagcatgg tgtagagtgc ggaccgaatg ccgtttttac ttttaaacgc gaaggttatg
      781 gtaagactga ctttgattta aaagacaccc ttcaggcatt agcatatatc ggaacatgga
      841 aactcttttt caaacactgg cgttttggtc ttgatgaata taaaagagct ttctccaaaa
      901 aattatttct tgaaagactg caaagattga ttccttcatt ggagatggat gacatcactc
      961 ccggacgtgc aggtgttaga gcaatggcac ttgatgcaaa tggaaatatg attgatgatt
     1021 ttaaaattga atacagcggc aatgccatac acgttttaaa tgccccttca ccggcagcaa
     1081 ctgcatcgtt agctattgga acagcagtta tggaaatggc agaaaagcat tttcagattc
     1141 attaaagcgg attttaaaag ttacttttac tgacataaac ctatctgttt tcaggaaaat
     1201 tactcattac cgcaaatgac aattatcatt ttagtcccaa cgcattgatt gaccttattc
     1261 tttaattttg cagagaatta ttaactaatt aataagcatt atgcgcacta caattctccg
     1321 gtttacattt ttagtttcaa gcatcttgtt gtttagcttt agcagccatg cacaggaaag
     1381 taaaacagac actattaaag tttatggtaa ctgtaatatg tgtaaagaaa aaattgaagg
     1441 ctccttaaaa gagaaagacg gcattactgc aaaaaactgg gataagaaaa ccaaactact
     1501 ggcagtaact tataatcctt caaaaatcac aaaagaacaa ataggacaaa aaattgcaga
     1561 tgttggctac gataatcagt atgcaacagc tactgagaaa gtatataaaa aactgcattc
     1621 atgttgtcag tacgacaggc ctaaacaacc tgccggtgat gaaaaaaata aatagacact
     1681 aaacaaacat atcaaaatga aaaagcaact ccttacactt attctttttg ccataacatc
     1741 tttaggtatg gcacaaacag caacagactt tacagcaaac gactgtaacg gcaattcgca
     1801 caatctcttt actgaattaa attccggaaa agtaattgtt ttatgttggg taatgccttg
     1861 tgcatcttgt accggacctt ctttaacaac ctataatgtt gttcagagtt ttcaggcttc
     1921 caatcctaat aaagttttta tgtacacctg tgatgatttt gcaaacacca attgtacttc
     1981 tttaaacagt tgggcaaacg gaattggtgt tatgcaacaa agctggtcgt tacgattttc
     2041 gaatgcagca attaacatga cgcattatgg ttcaccggga atgccaaaga ttgtagtttt
     2101 aggacctaat catgctgtat tttataattc gaacaatact gttaatgcaa ctaacctgca
     2161 aaatgctatt aacaatgctt tggcaacaac aggtttaaat gaaagcaatt tacctgcatc
     2221 atcattatct gtactgacaa
//
LOCUS       DAORQT010000267         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10070_length_2236_cov_4.266850, whole genome shotgun sequence.
ACCESSION   DAORQT010000267 DAORQT010000000
VERSION     DAORQT010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10070_length_2236_cov_4.266850"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK134_07885"
     CDS             <1..432
                     /locus_tag="PK134_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB38807.1"
                     /translation="TLYLLHTFGELCLSPIGLSMVAKLAPLRFASLLMGTWFLANSAS
                     NKFAGTLSSLYPPGQGEFKAAADKGIDLPSILNGSVTATADQLKVLAEAGIPHHFPMF
                     MGMQISSLYDFFMIFVIMSAAASVLLFIIYKWLVKMMHGVQ"
     gene            537..770
                     /locus_tag="PK134_07890"
     CDS             537..770
                     /locus_tag="PK134_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38808.1"
                     /translation="MKKLFTLMAVAAMFAMVSCGPSADEKAAAEKATQDSIANAEAEM
                     KAAEEAAMAASAAAATPDSAAAAAPADSAAAAH"
     gene            complement(800..979)
                     /locus_tag="PK134_07895"
     CDS             complement(800..979)
                     /locus_tag="PK134_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HRB38809.1"
                     /translation="MAKLTLIEVALFYINMQEIIVAIIAIAASVYLIYKFRSKSAGCD
                     KCSSGPQKSGQKKTT"
     gene            997..>2236
                     /locus_tag="PK134_07900"
     CDS             997..>2236
                     /locus_tag="PK134_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB38810.1"
                     /translation="MNFDRKDLSNDQLIKLYHELVRPRIIEEKMLVLLRQGKLAKWFS
                     GIGQEAVSVGAAMALHQDEYVLPMHRNLGIFSVRNIPLNRLFSQWMGKPGGFTNGRDR
                     SFHFGTQEYKIIGMISHLGPQCGIADGIALGNILKQNNKATIVFSGDGGASEGDFHEA
                     VNVAAVWDLPVIFIVENNGYGLSTPSSEQYRCKSFADKGIGYGIQGVSVDGNNILEVY
                     NTLNELLTDIRKNPRPIIFEARTFRMRGHEEASGTKYVPQQLFDEWGKKDPIANYENY
                     LLENKIISSSEVESIRAKIKTEIEENLKISYAESDPEVNTVNELRDMYAPSENTNITA
                     SGAVTEKRFLDAITDGLRQGMQRHKNLVIMGQDIAEYGGVFKATQGFVEEFGKPRVRN
                     TPITESSILGASLGLSINGFK"
BASE COUNT          696 a          412 c          461 g          667 t
ORIGIN      
        1 acattatact tgctgcatac atttggtgaa ctttgtctgt ctccaatagg actttcaatg
       61 gttgctaaat tagcaccgct gcgttttgca tctttgttaa tgggaacatg gttcttagcc
      121 aactctgctt caaataaatt tgccggtaca ctaagttcat tatatcctcc cggacaaggc
      181 gagtttaaag ctgctgcaga caaaggcatt gatcttcctt ctatacttaa tggctctgtt
      241 acagccactg ccgatcagtt aaaagttctt gcagaggcag gtattccgca ccattttcca
      301 atgtttatgg gtatgcagat ttcaagcctc tacgacttct ttatgatatt tgtaattatg
      361 agtgcagcag catcagtact gctgtttatc atttacaaat ggcttgttaa aatgatgcat
      421 ggtgtgcaat aacaattaat tgttaacaac ttagttgaat agttaataaa aaacaccttc
      481 atcatttatt aatcagcata cttttgtccc gtcaaatcaa acaacaaaaa agaaaaatga
      541 aaaaattatt cacattaatg gctgttgcag caatgtttgc aatggtatca tgcggtccaa
      601 gcgctgacga aaaagctgct gcggaaaaag caactcagga ttcaattgct aatgcagaag
      661 ccgaaatgaa ggctgctgaa gaggctgcaa tggctgcatc tgctgccgct gctactcctg
      721 attcagctgc tgccgctgca cctgcagatt cagctgctgc tgctcattaa ttttaaaaaa
      781 ttaaggaaaa gaaccaccgt caggtggttt ttttttgccc tgatttttgc ggaccagaac
      841 tgcatttatc acatccggca gatttgctcc tgaatttata aatcagataa acacttgcag
      901 caatggctat tatggcgaca attatttctt gcatattaat ataaaacaaa gctacttcaa
      961 ttaaagttaa ctttgccaca ctttttttat ttaacaatga atttcgacag aaaagatttg
     1021 agtaacgacc aactaatcaa actttatcat gagttggtgc gtcctcgtat aattgaggaa
     1081 aagatgcttg tcctacttcg tcagggaaaa ctggcaaagt ggttttccgg catcggtcag
     1141 gaagccgttt ctgtaggtgc tgccatggca cttcatcagg atgaatatgt tttgccaatg
     1201 catagaaacc ttggcatttt tagtgtacga aatattcctt tgaaccgttt gttttcgcaa
     1261 tggatgggca agcctggtgg attcaccaat ggtcgcgatc gctcttttca cttcggtact
     1321 caggagtata aaataattgg aatgatatcg catctcggtc cgcaatgtgg aattgcagat
     1381 ggaattgctc tgggaaatat tcttaaacaa aataataagg caacaattgt tttttcaggt
     1441 gatggtggtg ccagcgaagg cgattttcac gaggcagtga atgttgcagc tgtatgggat
     1501 ctgcctgtta tttttatagt agaaaataat ggttacggat tgtcaacacc atcgtccgaa
     1561 caatacagat gtaagagttt tgcagataaa ggaattggtt atggcattca aggtgtaagt
     1621 gttgatggca acaatattct ggaagtttat aatacactga atgaattatt gactgacatc
     1681 agaaaaaatc cacgtccaat aatttttgaa gcacgcacat tcagaatgcg cggtcacgaa
     1741 gaggcatcag gtaccaaata tgttcctcag caattgtttg atgaatgggg aaagaaagac
     1801 ccgatagcga attacgaaaa ttatctttta gagaataaaa taatttcttc ttcggaagtt
     1861 gaaagtattc gcgcaaaaat caaaacagaa attgaagaaa atctaaagat aagttacgca
     1921 gagtccgatc ctgaagttaa cactgtaaat gagctgcgcg atatgtatgc tccttctgaa
     1981 aacacaaaca ttactgcttc aggagcagtt actgaaaaaa gatttttaga tgccataact
     2041 gacggacttc gtcaaggtat gcagcgtcat aaaaacctag tcatcatggg tcaggatatt
     2101 gcagagtatg gcggagtgtt taaagcaaca caaggatttg ttgaagagtt tggcaaaccg
     2161 cgtgtccgaa atacacctat tacagaatca tcaatacttg gtgcaagttt aggtttatca
     2221 ataaacggct ttaaat
//
LOCUS       DAORQT010000268         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10091_length_2232_cov_5.594396, whole genome shotgun sequence.
ACCESSION   DAORQT010000268 DAORQT010000000
VERSION     DAORQT010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_2232_cov_5.594396"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK134_07905"
     CDS             <1..487
                     /locus_tag="PK134_07905"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRB38811.1"
                     /translation="IQHRYQHENFVIAGTSAGAMAMSNTMIYQGSSSGALIKGEVKIT
                     TGLALMGDVIIDSHFDKRGRFGRLAQAVASNPSCIGIGLGEDTGVLLTEGHLLEIIGS
                     GLVILIDGHQIRHNNIADLQDGVPISIENLIVHVLTKGNCYDLKSRKFYSELVKESKP
                     A"
     gene            790..>2232
                     /gene="dnaE"
                     /locus_tag="PK134_07910"
     CDS             790..>2232
                     /gene="dnaE"
                     /locus_tag="PK134_07910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB38812.1"
                     /translation="MPRFSHLHNHTQFSLLDGAADIKSLYKKAVKDNMPALAITDHGN
                     MFGVFNFVAEAAKYNTSENPNLIKPIVGCEFYLVENRHKRSFTKDDKDVRYHQLFLAK
                     NTIGYKNLVKLTSLGYMEGLYGKYPRIDKELVLKYHEGLIATTCCLGASVPKTILRKG
                     EAEGEKEFLWWLDIFGEDYYVELQRHDIPEQNTVNEVLIGFALKHNVKIIASNDSHYV
                     NREDYNAHDILLCINTGEKQSTEKFKDGDEDTSARDKRFAFYNDEFYFKDTSEMQQLF
                     SDRPEAIDNTNEIVDKIEPLKLKQDILLPHYQIPKGFESQDDYLKHLAWEGARKRYSE
                     ITPDIEERINFELFTVKTMGFAGYFLIVADFINHGKNIGVLVGPGRGSAAGSAVAYCI
                     GITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVVDKYGKNQVAQIIT
                     YGTMAAKLSIKDVARERELPVRDAIALAILG"
BASE COUNT          731 a          366 c          455 g          680 t
ORIGIN      
        1 tatacagcac agatatcaac atgaaaattt tgtaattgca ggtaccagtg ccggtgcaat
       61 ggctatgagt aataccatga tttatcaggg tagcagttca ggtgctttaa ttaaaggtga
      121 ggtgaaaatt actacaggac ttgcattgat gggcgatgtt attattgatt ctcatttcga
      181 taaacgtggg cgatttggcc gacttgcaca ggcagtagca agtaacccaa gttgtattgg
      241 tatcggtctt ggagaagata caggagtact gctcactgaa ggtcatttgc tcgaaattat
      301 aggttcggga ttggttatat taattgacgg gcatcagatt cgccacaaca atattgccga
      361 ccttcaggat ggtgtgccca taagcattga aaatctgatt gttcatgtac tcacaaaagg
      421 taattgttac gacctgaaaa gcagaaaatt ttattctgaa ttggtcaaag aaagcaaacc
      481 tgcatagttg aatttcattt cttcttacat ctttcttaca acacaatatt attggcatgg
      541 ctattgaaat gtttaaggca taacaataat cttcatttgc ttatggtcta aaacaaaagt
      601 gaacctcaaa taaaagcaac cggtatgtgc caacatcggt tgctttttca ttttagggca
      661 tgcacatctc tgaaagtcac atgggttttt gttattaaca aacaaagaat ttttgaatcg
      721 ttttattaat tttacatgcc acaatagcca aaccggttat tggattcaaa attcatactc
      781 agttttttca tgcctcgttt ctctcattta cataatcata cacagttctc tttgctcgat
      841 ggtgcagcag atattaagtc gctttataaa aaagcagtaa aagataatat gccagctttg
      901 gcaattaccg atcacggaaa catgtttggt gtatttaatt ttgttgccga agctgcaaaa
      961 tacaatacta gcgaaaaccc caatttaatt aaacctattg taggttgcga attttatctg
     1021 gtagaaaatc gtcataaacg aagctttacc aaagacgata aagatgtacg ttatcatcag
     1081 ttatttcttg caaaaaatac tattggatat aaaaaccttg tcaagttaac ctcgctaggt
     1141 tatatggaag gtttgtatgg aaagtatcca cgtattgata aagaattggt gcttaaatat
     1201 cacgaaggcc ttattgcaac aacatgttgc ctgggtgcaa gtgtgcctaa aacaatttta
     1261 cgcaaaggtg aggccgaagg agaaaaagaa tttttatggt ggctcgatat ttttggagaa
     1321 gattattatg tagagttaca acgtcatgat attccggaac agaatacagt taatgaagtt
     1381 cttattgggt ttgcattaaa acacaatgtg aaaattattg catcaaacga ttctcattat
     1441 gtaaatcgtg aagactataa tgcacacgac attttgcttt gcatcaacac cggtgagaaa
     1501 caaagcacag aaaaatttaa agatggcgat gaagatacat ctgcacgcga taagagattt
     1561 gctttttata acgatgagtt ttattttaaa gacacatcag aaatgcaaca actttttagt
     1621 gacagacctg aagcaattga caataccaat gaaattgtag ataaaattga gccattaaaa
     1681 ctgaagcagg atattctttt accacactat cagattccaa aaggcttcga aagtcaggat
     1741 gattatctga aacatcttgc atgggaaggt gcacgcaaac gttatagcga aatcacccct
     1801 gatattgaag agcgcataaa ctttgaattg tttacagtaa aaacaatggg ttttgccggc
     1861 tactttttaa ttgtcgctga ttttattaat catggcaaaa atattggtgt gttggtcgga
     1921 cccggcagag gtagtgcagc aggctctgca gttgcctatt gtattggcat caccaacatt
     1981 gatcctatta agtataattt actgtttgaa cgttttttaa atccagacag aaaatcaatg
     2041 cctgatatag atactgattt tgatgatgaa ggtcggcaga aggtaataga ttatgtagtt
     2101 gacaagtacg gaaaaaatca ggtagcacaa attattactt acggcacaat ggctgcgaaa
     2161 ttgagtatta aagatgtggc acgagagcgt gagttgccgg tgcgtgatgc gattgcatta
     2221 gcgatcctgg gg
//
LOCUS       DAORQT010000269         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10226_length_2213_cov_4.822057, whole genome shotgun sequence.
ACCESSION   DAORQT010000269 DAORQT010000000
VERSION     DAORQT010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10226_length_2213_cov_4.822057"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PK134_07915"
     CDS             <1..1309
                     /locus_tag="PK134_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38813.1"
                     /translation="LTAMYYAFRKLIPYTILFAVLIFTGINSYFLYFASQTYSEAFFC
                     FLQALLFIVIRPILSASKEDNIPIAKYLVFGIILTLLSLTKNITVGAIAAVALYFVLN
                     RDFKKLAYAFGSFVVVRGIFEVIKSFIWGSQNQFSNQYQILIQKDPYNASKGVDDFGG
                     FVDRLFSNTSIYIGKRLYQILGFVSPDNTKTNAGLVLITFVFVGIGLYFIFKNKNKIM
                     QFTFLYVLLMAGLTFFVLQTSWDQPRMVLVYVPFILMIIFYGLYYAFQNTPALRSSIF
                     LIVSIIIIMSSLMKTVDKSVTNLPILKKNMGGDIYYGYTEDWTNFLKLSRYCGDSLPE
                     SAYVVSRKAPMSFIYSKGKSFYPIYTVPSTDPDSVLALLKENKVTHVLIASLRRNPKK
                     NDGYVINTLHRMMGPVGQKYPDKLKLVKQVGTSEPSYLYEIKY"
     gene            1309..2040
                     /locus_tag="PK134_07920"
     CDS             1309..2040
                     /locus_tag="PK134_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB38814.1"
                     /translation="MSDRISNELEHGKKLLEGGAEAIWNWDSPAGKVRADRRADLIAK
                     YSLADKSSTVLEIGCGTGLFSGKFYQRTGAKIIATDLSPELLNVAKEKYPAVEFVLAD
                     AMKLQFDANTFDVVFGSSVLHHLEFERSLNEILRVLKPGGRMVFAEPNMINPQIFVQK
                     NIPFIKKWLGDSPDETAINRWNFSKLLEQKGFNQINIFPYDFLHPVVPSFLISLVSGI
                     GQVVEKIPIAKEIAGSVIIVAEKPR"
BASE COUNT          698 a          354 c          392 g          769 t
ORIGIN      
        1 tttaactgca atgtattatg catttcgcaa actaatacct tacactatat tgtttgcagt
       61 tttaattttt accggaatta atagctactt cttatacttt gccagccaaa cctactccga
      121 ggctttcttt tgctttttac aagcattgct atttattgtt atacggccta ttcttagtgc
      181 ctctaaagaa gataacatac ccattgctaa atatttggtt tttggaatta ttcttacttt
      241 gttgtcacta acaaaaaata taactgtagg tgcaattgct gcagtagcat tatattttgt
      301 cctcaatcgc gattttaaaa aattagctta tgcctttggt tcttttgttg tagtgcgtgg
      361 catttttgaa gttattaaat catttatctg gggttcacaa aatcaattca gtaatcagta
      421 tcagatttta attcagaaag acccttacaa tgcatcaaaa ggggtagatg attttggcgg
      481 atttgttgac agactttttt ctaatacaag tatttatatt ggtaaaaggc tttatcagat
      541 tttgggattt gtttcaccgg acaataccaa aaccaatgca ggtctagtgt tgattacatt
      601 tgtttttgtt ggcattggtt tgtatttcat tttcaagaat aaaaacaaaa tcatgcaatt
      661 tacatttctg tatgtattgt tgatggcagg attgaccttc tttgttttac aaacctcatg
      721 ggatcaacca agaatggttt tagtttacgt gccatttatt ctgatgatta ttttttatgg
      781 actatactat gcttttcaaa acactcccgc actacgcagt tcaatttttc ttattgttag
      841 tatcattata ataatgagta gtctgatgaa aactgttgat aagtctgtaa ccaatttacc
      901 gatattaaaa aagaatatgg gtggtgatat ttattacgga tataccgaag attggacaaa
      961 ctttttaaaa ttaagtcgtt attgcggtga cagtctccct gaatctgctt atgttgtttc
     1021 acgaaaagca cctatgtcat ttatctattc caaaggcaaa tcattttatc cgatatatac
     1081 agtaccaagc actgaccctg actctgttct tgcattgtta aaagaaaata aagtaacgca
     1141 tgtgttaatt gcaagtttaa ggcgaaatcc aaaaaagaat gacggctatg tcatcaacac
     1201 attgcataga atgatggggc ctgttggaca aaaatatcct gataaattaa aattagtgaa
     1261 gcaggttgga actagtgagc cttcttacct atatgaaata aaatattgat gagtgataga
     1321 atttcaaacg aattagaaca cggcaaaaaa ctacttgagg gtggtgcaga agcaatatgg
     1381 aactgggaca gtcctgcggg aaaggttaga gctgacagac gtgcagattt aatagccaaa
     1441 tattcactcg cagataaaag cagcacagta ttagaaatag gttgtggaac aggacttttt
     1501 tcaggcaaat tttatcagcg tacaggtgct aaaattattg caactgacct ttcacctgaa
     1561 ttactaaatg ttgcaaaaga gaaatatccc gcagtagagt ttgtgttggc agatgccatg
     1621 aaactacaat ttgatgcaaa tacatttgat gtagtattcg gaagttcagt attacatcac
     1681 ttagaatttg aacgatcgct gaatgaaatt ttaagagtat taaaaccggg cggccgtatg
     1741 gtttttgctg agcctaacat gattaatccg cagatttttg ttcagaaaaa tattccattc
     1801 attaaaaaat ggcttggtga ttcaccggat gaaactgcta tcaaccgatg gaactttagt
     1861 aagttgcttg aacaaaaagg atttaatcag attaatattt ttccttatga ttttttacat
     1921 cctgtagtgc catcattttt aatttcattg gtgagtggta ttggtcaggt agttgaaaaa
     1981 atacctattg ctaaagaaat tgcaggttct gtaattatag ttgcagaaaa acctcgttaa
     2041 ttaatcaaaa cttttttagc tgttattgat ctcccctgaa tcaattgaac agtataaatt
     2101 ccactattca atggagctgt ggcaatttgc tttcttgaat tatctactga aattctaaat
     2161 acttctttcc cctgcaaatc aaatactgtt aaaatgcctt ctccttttaa ttg
//
LOCUS       DAORQT010000270         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10377_length_2193_cov_5.272685, whole genome shotgun sequence.
ACCESSION   DAORQT010000270 DAORQT010000000
VERSION     DAORQT010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10377_length_2193_cov_5.272685"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK134_07925"
     CDS             complement(<1..676)
                     /locus_tag="PK134_07925"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRB38815.1"
                     /translation="MSSTDKKIYFISDFHLGAPTSEESLVRERKICQWLDTIKADVSI
                     LFILGDVFDFWFEYRHVVPKGYVRILGKLAEIADKGIPIYLFTGNHDMWMFDYLPKEL
                     GIKLYRQPEEFVFDNKKFLIGHGDGLGPGDHGYKFIKKVFANKFCQWLFARLHPNFGI
                     SLANFWSRKSRQHTGHHDAKYLGDDKEFLVQYIIETEKSKHFDYYIFGHRHLPIDKQI
                     GDSHYIN"
     gene            867..1229
                     /locus_tag="PK134_07930"
     CDS             867..1229
                     /locus_tag="PK134_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38816.1"
                     /translation="MKKLVFMVLLMLTKVAFCQENINGQKLKDYVFGVPQMDLVKQLP
                     DFKNSLKTINGLEFHGFCESQHLLMVRMPESSMDAFSELMDKMELSFNVKKQASFQIA
                     EKVCNAKEEILYSKSINQ"
     gene            1232..>2193
                     /locus_tag="PK134_07935"
     CDS             1232..>2193
                     /locus_tag="PK134_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38817.1"
                     /translation="MKTTIKYLTLFFALITINVINVSAQCNVGTLAGTITPTINWQTV
                     AVDGRDYQNFAATAGYKYIFSFCAADGGSTSRNTEITINDNAGVIIPGAYNNDFCGSA
                     SYLEWTCVTTGTYRVLTTRRTCATQSNLGTMAYRLDFSYNCPAGLGTGYTSVASLPYT
                     MASGTTCSQKNDVNAIRMEACGTANYYEGEDMVWSFTPATTGTVTVSFNSGASNTTLS
                     IFDACPLKGNGAGCVVNQQGNGNKSVSFCATAGTTYYAVIDCRTSTGCFAFTNFTISA
                     PVVNYNLGNGVTNVASLPFIANNQSTCGNGNDLTSGNTPACGNG"
BASE COUNT          714 a          387 c          381 g          711 t
ORIGIN      
        1 aattaatata gtggctatcg cctatttgtt tatcaattgg cagatgccta tgtccaaata
       61 tataataatc aaaatgtttt gatttttcag tttcgataat atactgaacc aaaaattctt
      121 tatcatcacc taaatactta gcatcatgat gcccggtatg ttgtctgctt ttacggctcc
      181 agaagttggc taaagaaatt ccaaaatttg gatgcaaccg ggcaaacagc cattgacaaa
      241 acttattggc aaagactttt ttgatgaact tatatccatg atcaccgggt cctaagccat
      301 caccgtgacc gattaagaac tttttgttat caaatacaaa ttcttccggc tgacggtaaa
      361 gtttaatacc caattctttt ggcaaataat caaacatcca catatcatga ttgcctgtaa
      421 acagatagat tggtattcct ttgtcggcta tttctgccaa ttttcctaaa attcttacat
      481 atccttttgg cacaacatga cggtattcaa accaaaaatc aaatacatca ccaaggataa
      541 aaaggattga aacatcagcc ttaattgtat ctaaccattg gcagatttta cgctctctca
      601 ccaaactctc ttcacttgtt ggtgctccga gatgaaagtc actaataaaa taaatttttt
      661 tatctgtgct gctcaagttt caaaaaataa tttgctgcta atttctgtaa taaatatcaa
      721 actacaaggt aaacggctta aatgattata aaactatcaa cattaagcct aaaacattac
      781 acaccttaca aaagtaattg ctttggtcta actataacct tttggtaaag ttgggcgtat
      841 ataagcactg ttaaaatcat tttaatatga aaaaacttgt attcatggta ttgttgatgc
      901 tgacaaaagt tgccttctgt caggaaaaca ttaacggaca aaaattaaag gactatgttt
      961 ttggagttcc gcagatggat ttggtaaaac aacttcctga ttttaaaaat agtcttaaaa
     1021 caattaatgg tcttgagttt catggtttct gtgaaagtca gcatttacta atggtacgaa
     1081 tgccagaaag ttctatggat gctttttcag agcttatgga caaaatggaa ttaagtttta
     1141 acgtaaaaaa acaagcttca tttcagatag cagaaaaagt ttgtaatgca aaagaagaaa
     1201 tcttatacag taaatctata aatcaatgag tatgaagact acaatcaaat acttaacttt
     1261 atttttcgca ttgattacaa tcaatgttat taatgtaagt gctcaatgta atgttggcac
     1321 attagccggc actattactc ctacaattaa ttggcaaact gttgctgttg atggtagaga
     1381 ttatcagaat tttgctgcaa ctgcaggtta taaatacatt ttttcatttt gtgctgctga
     1441 tggtggaagt acatcaagaa atactgaaat tacaattaac gacaatgcag gggtaatcat
     1501 tcctggtgcc tataataatg acttttgtgg ttcagcctct tacttagaat ggacatgtgt
     1561 cacaaccggc acttatcgtg ttttgaccac aagaaggact tgtgctactc agagcaactt
     1621 aggtacaatg gcatatcgct tagattttag ttataattgt cctgcaggtt taggtaccgg
     1681 ttatacaagt gtagcttctt taccttatac catggcatca ggcaccacat gttcacaaaa
     1741 gaatgatgtt aatgcaatcc gtatggaagc atgtggtaca gccaattatt atgaaggcga
     1801 agatatggta tggtccttta ctcctgcaac tacaggtaca gttacagtat catttaattc
     1861 aggcgcaagc aatactactc tctctatatt tgatgcatgc ccgttaaaag gaaatggtgc
     1921 aggctgtgta gtaaatcagc agggcaacgg gaataagtca gtttcatttt gtgcaactgc
     1981 cggtacaact tattatgcag taatagactg tcgtacttct actggttgtt ttgcatttac
     2041 aaactttact attagtgctc ctgttgtaaa ttacaattta ggcaatggcg ttaccaatgt
     2101 tgccagttta ccatttattg caaacaacca atcaacatgt ggtaatggta acgatctgac
     2161 ttcaggtaat acacctgcat gtggaaatgg ttt
//
LOCUS       DAORQT010000271         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10457_length_2181_cov_3.744120, whole genome shotgun sequence.
ACCESSION   DAORQT010000271 DAORQT010000000
VERSION     DAORQT010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10457_length_2181_cov_3.744120"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK134_07940"
     CDS             complement(<1..456)
                     /locus_tag="PK134_07940"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38818.1"
                     /translation="MSKVFLTLVTVAVMSINLSSAQCPPLGAFSGMTGDFKACPGMSL
                     TYTLTPVTNAISYTWNLPPGATISGQNPYTTSSNVVTVDYGPLFSAPGSLCVTAYNGC
                     VNSAPYCKALTPAGAPSGASTISGSIFACPGVTETYSVTNQVNRVFDWTI"
     gene            750..>2181
                     /locus_tag="PK134_07945"
     CDS             750..>2181
                     /locus_tag="PK134_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRB38819.1"
                     /translation="MAKRKNLQAEANTIDTVEEKPEVKRKTVKPKPEKLNLKWLANEQ
                     LHQIIGLFCILISIFLTIAFISYLFTWKIDFDKVKNGWWSLLTDRDLKVENWLGKTGA
                     LSANSFFYNAFGLSSFLFVFILFLSGFKLVFRHALLPFGRTIRISLFTLLFVSIACGY
                     IFKNTSLFLFTGGTFGFETSEWLQSTIGNAGTGLLLGFTLLTFSIITFKFKLKLPAIG
                     EKNIQQEEEDALLTEEQSENKINTISDEITKEAEDLKNNVPVSFELEPDMDKAISDLM
                     NSGDEQKKVPDSNNLELELNIETPSLEKDEPVVKESNIPLAIENLNGSEDTTEASEEN
                     DNNELPDQVTLGEYDPTLDLSSYQYPVLDLLENYQSKSKLTHEELKAELETNKNKIVE
                     TLGHYNIGIEKIKATVGPTVTLFEIVPQAGVRISKIKNLEDDIALSLSALGIRIIAPI
                     PGKGTIGIEVPNQSPEVVPMKAVLGSE"
BASE COUNT          753 a          376 c          380 g          672 t
ORIGIN      
        1 aatagtccaa tcgaaaactc tgtttacctg attggtgaca gaataagttt cagtcactcc
       61 cggacaagca aaaatagaac ctgaaatagt tgatgcccct gatggtgcac ctgcaggtgt
      121 cagcgctttg caatatggtg ctgaattaac acaaccatta taagcagtta cacagaggct
      181 tccaggagca gaaaataaag gaccgtaatc tactgtaaca acattcgatg aagttgtgta
      241 aggattttgt ccgctgatag ttgcacccgg aggcaagttc cacgtatagc tgatggcatt
      301 agttacaggt gtaagggtgt aggttagcga cattccgggg catgctttaa aatcgcctgt
      361 cattccggaa aacgctccta atggagggca ttgtgctgat gataaattaa tgctcattac
      421 agcaacagtt actaaagtca aaaagacttt actcattcta attagtgttg attttgcatt
      481 tttcataata atatgcggct atttgatttg aattgtttac gcaataaatt ggttaaagtt
      541 ttagtgatgc atttctttat gcaatcttac caatgcttta gtaatacaaa aatgcattac
      601 aaaataggtt gattttatga aaactatcca cataacaacc catttatcca caataaaatg
      661 tttattacat ggttttgttt actgtaatgt caacttagtt tagatttaat ttagctcaat
      721 taaatatttt ctttcacata aaaaacaaaa tggccaaacg caaaaattta caggctgaag
      781 caaacaccat tgacacggtc gaagagaaac ctgaagtcaa aagaaaaact gtaaaaccta
      841 aaccagaaaa actcaactta aaatggttag ctaatgaaca gctacatcaa ataatcggac
      901 tattttgtat tctgataagt atttttctaa ccatagcttt tatctcttat ttatttacct
      961 ggaaaattga ttttgataaa gttaaaaatg gctggtggtc attactgaca gaccgtgatc
     1021 tgaaagttga aaactggctt ggcaaaacag gagctttatc tgcaaattca tttttttaca
     1081 atgcttttgg gttaagtagt ttcttatttg tttttatatt gttcttatcc ggatttaaat
     1141 tggttttccg acatgcttta ctaccatttg gacgcaccat aagaatttct ctatttacac
     1201 tgctctttgt ctcaattgca tgtggatata tttttaaaaa taccagtctg tttttattta
     1261 ccggtggcac attcggattt gaaacttctg aatggctaca atccacaata gggaatgccg
     1321 gtaccggatt attacttgga ttcacactac ttactttctc aatcattaca ttcaagttta
     1381 aattgaaact tccagcaatt ggtgaaaaaa atattcaaca ggaagaggaa gatgctttat
     1441 taactgaaga acaatcagaa aataaaataa acaccatttc agatgaaatt actaaagaag
     1501 cagaagattt aaaaaacaat gttcccgtta gttttgaatt agaacctgat atggacaaag
     1561 ccatttcgga tttaatgaat tctggtgatg aacaaaaaaa agtacctgat tccaataatc
     1621 ttgaattaga attaaacatc gaaacacctt ctcttgaaaa agacgaaccc gtagttaaag
     1681 aaagtaatat tccacttgca attgaaaatc tgaatggctc tgaagataca actgaggcat
     1741 cagaagaaaa cgacaacaac gaacttcccg atcaggttac tcttggcgag tatgacccga
     1801 cacttgattt atcctcttat caatatcctg tattagattt actggaaaat tatcagagta
     1861 aatctaaact tacacacgaa gagttaaaag ctgagttgga aaccaataaa aacaaaattg
     1921 ttgaaacact tggtcactat aatattggta ttgaaaaaat caaagcaaca gtaggaccta
     1981 cagtaactct atttgaaatt gtgccacaag ccggtgtacg tatttcaaaa atcaagaatc
     2041 ttgaagatga tattgcttta agtctttcag ctttgggtat tcgtatcatt gcccctattc
     2101 cgggcaaagg aactattggc attgaagtac ctaatcaatc gcctgaagta gtaccgatga
     2161 aagcagtatt aggctctgag a
//
LOCUS       DAORQT010000272         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10532_length_2171_cov_10.002836, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000272 DAORQT010000000
VERSION     DAORQT010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_2171_cov_10.002836"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2171)
                     /locus_tag="PK134_07950"
     CDS             complement(<1..>2171)
                     /locus_tag="PK134_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRB38820.1"
                     /translation="AMIPFFGVAQITDNFSDGDFTANPAWSGDNSQFIVNASQQLQLN
                     STGAATSYLALTTSLPTLDNTSWQFYIKLNFAPSSSNYARIYLVSDQQNLKGALNGYY
                     VQFGETGSNDAIEIFKQTGTTSVSVARGTNGFIAAASTISVKITRDNAGLWSIYADAA
                     AGTNFTLQSSGTDATINSGSYFGVSCTYTSSNATGFFFDDFSIPFIADITPPTLTSAI
                     PLSNTALDVKFSELVDLTTSQTTTNYAIQGLGTPATAVRDISDFSLVHLTFSTPFTSG
                     TAYTLSVSNVSDLNSNVIAAGSTIGFTWNAPVTAQPFDVVINEIYFEPIVSAPLPNAE
                     FVELYNRSNKILSLNGWTLSDGSTSVASIGAVTLQPSAYIIVCKNSDTTSFIPYGNTT
                     GTSSFPSLNNDVGDDLKLTDNNGTLIDRVIFSDDNYNDDSKKTGGFTIERIDADFTCN
                     SRFNWHASNDAQGGTPGKVNSVKGTYSDTTPPQAVDAYPLTTTLIRITFSENPDNAIA
                     SQTSSYIIDNGIGQPASVLLSDNHAFLTLNNALQTGIVYHVNLNSSIADCPGNKITGE
                     LSLKIGIAETAVAGDVLINEILFNPKTGGNDFVELYNNSSKIIDLKNLKVATTDNNNL
                     ITTNYSVSDGGYLLFPKEYIALTSDVASLQSFYPNAATNNLLKASLPGFNDDMGVCVI
                     TDFALNRIDQLNYNKRWHFPLIDDQNGVSLERIWFNKGTQD"
BASE COUNT          640 a          395 c          425 g          711 t
ORIGIN      
        1 atcctgtgtg cctttattaa accatattct ttctaatgaa acaccattct gatcgtcaat
       61 gagtggaaaa tgccaacgct tattatagtt aagctgatca attctgttca atgcaaaatc
      121 ggtaatgaca caaacaccca tgtcatcatt aaaacccggc agtgatgctt ttagtaaatt
      181 atttgttgca gcattgggat aaaaactttg cagtgatgca acatcagaag taagtgctat
      241 gtattccttc ggaaataaaa gataccctcc atcactaaca ctataatttg ttgttatcag
      301 attgttgtta tcagttgttg caactttaag atttttaagg tcaattattt ttgatgagtt
      361 gttgtaaagc tccacaaagt catttcctcc tgtttttggg ttaaacaata tttcgtttat
      421 cagcacatca cctgcaacag cagtttctgc aataccaatt tttaaactaa gttctcctgt
      481 tattttattt cccggacagt cagcaattga tgagtttaaa ttaacatgat aaactattcc
      541 ggtttgcaat gcattattta gcgttaaaaa ggcatgattg tctgaaagta atactgatgc
      601 aggctgacct attccattgt caataatata acttgatgtt tgtgatgcaa tagcattgtc
      661 aggattctcg ctaaatgtta ttcttatcaa tgttgtggta aggggataag catcaactgc
      721 ctgtggtggg gtggtatcgc tgtaagtacc tttaacactg tttacttttc ccggtgttcc
      781 gccttgcgca tcatttgatg catgccagtt aaatcttgaa ttacaagtaa aatcagcatc
      841 aattctttca atggtgaagc ctcctgtttt tttgctgtca tcgttgtagt tatcatcact
      901 gaaaattact ctgtctatca atgtgccatt gttatcagtc agtttaaggt catcaccaac
      961 atcattattt aacgaaggaa atgaacttgt ccctgttgta ttaccatagg gaataaatga
     1021 tgttgtgtca gaatttttac agacaatgat atatgcactt ggttgaagtg ttactgcgcc
     1081 aatacttgcc acggaggttg acccgtcaga taatgtccag ccattgagtg agagaatttt
     1141 gttacttctg ttgtacagct caacaaactc tgcattaggc agtggtgccg acacaatagg
     1201 ttcaaaatat atttcattaa tgacaacatc aaaaggctgt gctgttacag gagcattcca
     1261 tgtaaagcct atagtgcttc ctgcagcaat tacattgcta ttaagatcag atacattact
     1321 tacagagagc gtgtatgcag tgccacttgt aaatggagtg ctaaatgtaa gatgtactaa
     1381 tgaaaaatca cttatatcgc gtactgcagt tgccggagtg cccaaaccct gaattgcata
     1441 attcgttgta gtctgtgatg tagtcaggtc aacaagctca gaaaatttta catctaaagc
     1501 agtattcgat aacggaatag cggaagttag tgttggtggt gtgatgtctg caataaatgg
     1561 aatagagaaa tcgtcaaaga agaagcctgt ggcattggat gaagtgtaag tacaactcac
     1621 accaaaataa cttcctgaat taattgttgc atcagtacct gaagattgta aagtaaaatt
     1681 tgttcctgca gcagcatctg catatatact ccataggcct gcattatctc ttgtaatttt
     1741 tacactgata gttgatgccg ctgcaataaa tccattggtg cctcgtgcta ctgatacaga
     1801 ggttgtacct gtttgtttaa aaatttcgat ggcatcgttg ctgcctgttt ctccaaactg
     1861 tacataatag ccgttgagtg caccttttaa gttttgttga tcgctcacca aataaattct
     1921 tgcataatta cttgacgatg gcgcaaagtt gagctttata taaaattgcc aagatgtatt
     1981 atcgagtgta ggcaggcttg ttgttaatgc cagataggaa gttgctgcac cggtgctgtt
     2041 gagttgcagt tgctgtgatg cattgactat aaactgactg ttgtcgccac tccatgcagg
     2101 attagcagta aaatcaccat cagaaaagtt atctgttatt tgtgcaacac caaaaaatgg
     2161 aatcattgcg a
//
LOCUS       DAORQT010000273         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10549_length_2169_cov_6.265374, whole genome shotgun sequence.
ACCESSION   DAORQT010000273 DAORQT010000000
VERSION     DAORQT010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10549_length_2169_cov_6.265374"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PK134_07955"
     CDS             complement(<1..459)
                     /locus_tag="PK134_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HRB38821.1"
                     /translation="MSIASASFAENKDLKEQSANPLLQPWTGPYNGVPAFDKVKEEYF
                     IPAFEAAFAEKKAEIQKIASNKEAPNFKNTIEAMEKAGATLNRVRAIYDIWSSNLNTP
                     VMTAIQEKIEPQIAAFNDEVIQNKPLFLRIEKVCSDTVGKKYSVEQHQLLK"
     gene            complement(613..>1943)
                     /locus_tag="PK134_07960"
     CDS             complement(613..>1943)
                     /locus_tag="PK134_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38822.1"
                     /translation="LNNTINPGAMATLDVVNTATTPFLVKIKPTAPVNSNVVFKLSFT
                     DGNLIVNQYFTVVVNVDYINVNINEVATTITSKGRLFYYGINQTSGLGFDFNGDNLVY
                     DGGLMIGNNNLVSDNCRDGSNFDDDFQKQIAIKAIIPSVKSEFDLTTNFNDNNVTAAN
                     RLKVLVRHNTYAWSSPGNSRFIIVEYHIKNTGTNALSTLYAGICADWDIQTYANNKSD
                     VDAVNKMGYSWCTDNGGLYAGVKLLSATPFNSYAIDNVTGGAGGVDATSDFTNSEKYT
                     TLSTSRAQAGNTLPAGNDVIQVVSSGPFNLAGGDSITVAFALIAGDSLADIQQSAANA
                     QIKYDGILTSVQENIAHGSETQLLNPYPNPNSGNELNIPFYIAKNQINSVNISLTDMT
                     GRTVVDLQNIQTISGMNIQTVNMAGLSNGIYLIKLESAGKIYTGKISVQH"
     assembly_gap    1944..1961
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1962..>2169)
                     /locus_tag="PK134_07965"
     CDS             complement(<1962..>2169)
                     /locus_tag="PK134_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38823.1"
                     /translation="DKLGTGRVNLYNAVTQSPKGVRMDNISLSDNNDLAFVAGDTLRI
                     TGDVLNLLAPLSNLTVTLSTVSSSV"
BASE COUNT          650 a          393 c          365 g          743 t
ORIGIN      
        1 ttttaacaac tgatgttgtt ctacactata ttttttccca acagtatcgc tacacacttt
       61 ttctattctt aaaaacagcg gtttattttg aataacttca tcgttaaatg ctgcaatctg
      121 tggttcaatt ttttcctgaa tagcagtcat cacaggtgta ttcagattcg atgaccatat
      181 atcataaata gctcttactc tatttaaagt agctcctgct ttttccattg cttcaatggt
      241 atttttaaaa tttggagctt ctttgtttga agctattttc tgaatctctg cttttttctc
      301 tgcaaaagcc gcttcaaaag caggaataaa atattcttct ttaactttat caaatgccgg
      361 aacaccattg tatggccctg tccagggttg taataatgga ttagccgatt gttcttttaa
      421 atctttgttt tcagcaaaag aagcagaagc tatactcatg attgtaagta ttaaaattga
      481 tttctttttc atcatacagt aattaatgat tttgaatttg tattaatctc ttggctaagg
      541 tagaaaaatc aaaccacaca gaacgaccat tgtctgtgtg gtttgaaata ttaattatgg
      601 ttaatttaaa aattaatgct gtacagaaat ttttccggtg tatattttgc cggcactttc
      661 taatttaatc agataaatac cattgcttaa ccctgccatg ttaactgtct gtatattcat
      721 tcctgaaatt gtctggatgt tttgaagatc aacaactgtt cttcctgtca tatctgtcag
      781 actgatattt acagaattta tttgattttt tgcaatataa aaaggtatgt ttaattcatt
      841 tccactgtta ggattagggt agggattcag taattgtgtt tctgatccat gagcaatatt
      901 ttcctgaaca gaagttaaaa taccatcata ttttatttgt gcattagcag cactttgctg
      961 aatatcggca aggctgtcac ctgctatcaa tgcaaaggca actgttattg aatcaccacc
     1021 ggcaagatta aatggtcctg aacttaccac ctgaatcacg tcattacctg caggaagtgt
     1081 gtttcctgcc tgcgcacgac ttgtgcttag tgtagtatat ttttctgaat tggtaaaatc
     1141 acttgttgca tcaacaccac cggcaccacc ggttacattg tcaatagcat aagaattaaa
     1201 tggtgttgct gacaacaatt ttactcctgc ataaagtccg ccattatctg tacaccacga
     1261 ataccccatt ttatttacag catcaacatc agacttattg ttggcatagg tttgaatatc
     1321 ccaatcggca caaataccgg catagagggt actcaatgca ttggtgcctg tatttttaat
     1381 atgatattca acaatgataa aacgcgagtt gcccggtgaa ctccatgcgt atgtgttatg
     1441 tctgactaaa actttcagtc tgtttgcagc agtgacatta ttgtcgttaa aatttgttgt
     1501 caggtcaaat tctgatttta cagagggaat tatagcttta atagcaattt gtttttgaaa
     1561 atcatcatca aaattagaac cgtcacgaca gttgtcagaa acaagattgt tgttgccaat
     1621 catcaatcca ccatcataaa ccaaattatc accattaaaa tcaaaaccta atcctgatgt
     1681 ctgattgatg ccataataaa acaaacgtcc tttgctggtt atggttgttg ccacttcatt
     1741 gatgttaaca ttgatgtaat ctacatttac tacaacagta aaatattggt taacgataag
     1801 gttgccatca gtaaaagaaa gtttaaacac aacatttgaa tttaccggag ctgtaggttt
     1861 tattttaact aaaaatggtg tggtggcagt gttcaccaca tcaagagttg ccatagcacc
     1921 gggattgatg gtgttattca aaannnnnnn nnnnnnnnnn nttacactac ttgatactgt
     1981 tgaaagtgta actgtaagat ttgaaagagg tgcaagaagg tttagcacat cacctgtaat
     2041 tcgtaaggta tcaccggcaa caaatgcaag gtcattgtta tcagacaggc tgatattatc
     2101 catacgcact cctttagggc tttgtgtaac tgcattataa agattcacac gtccggtacc
     2161 taatttatc
//
LOCUS       DAORQT010000274         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10592_length_2165_cov_4.694787, whole genome shotgun sequence.
ACCESSION   DAORQT010000274 DAORQT010000000
VERSION     DAORQT010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10592_length_2165_cov_4.694787"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK134_07970"
     CDS             <1..954
                     /locus_tag="PK134_07970"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HRB38824.1"
                     /translation="VVLLLETVQAVKEKFAAEHIINVLIGKDDKAIKDYQHNKLEVFG
                     EGMDQDAKFWMAVVRQALLAGLLFKDIDNYGLLKLSDEGKKFMDKPHSFKITQDHSYE
                     SADSDEDDEPDGGGGGAAADETLFAMLKDLCKQIAKQKNLPPFVIFQEPSLEEMAIQY
                     PINMEEMKNITGVGAGKAGKYGKPFVDLIKKYVEENEIERPQDFIVKSVVNKSGLKVY
                     LIQNIDRKISLDDMAEAKGIPLGDIITELESIVASGTKIDLNYYLNEVIDADRQQDVF
                     DYFRTAESDSAEDALLELGENDYTIDEIRMMRVKFMSELGN"
     gene            1139..1426
                     /locus_tag="PK134_07975"
     CDS             1139..1426
                     /locus_tag="PK134_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38825.1"
                     /translation="MSRKLAVYTFNIYLCKKRDMKTLSLKLDDKIFNETEEITSKLKL
                     ARNRYINEAVSIYNTLNKRRLLKKQLAKESKLTSKDSMDILREFEKLMDEN"
     gene            1416..1751
                     /locus_tag="PK134_07980"
     CDS             1416..1751
                     /locus_tag="PK134_07980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRB38826.1"
                     /translation="MKIKQFDIWLADLNPSIGTEPGKKRPVVIVQTDLLNESHLSTLI
                     CTITTNVQKEILLLRVHLRKGQLEKLSDILVDQVRTIDNKRLIKKLGHLNKDQILKLK
                     TNLRIVLDL"
     gene            2048..>2165
                     /locus_tag="PK134_07985"
     CDS             2048..>2165
                     /locus_tag="PK134_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRB38827.1"
                     /translation="MQSNVGLVIEERAADIGNFMVGRLLPFRQKRKVGPFVFI"
BASE COUNT          783 a          368 c          403 g          611 t
ORIGIN      
        1 gtagtattgc tgctcgaaac tgttcaggct gtaaaagaaa aatttgctgc cgagcacatt
       61 atcaatgttc tcatcggaaa agatgacaaa gccataaaag actatcagca taataagtta
      121 gaagtctttg gcgaaggaat ggatcaggat gcgaaattct ggatggccgt tgtgcgtcag
      181 gcattgcttg caggactgct cttcaaagac attgataact atggattgct taagttgagt
      241 gatgagggta aaaaatttat ggacaagccc cacagtttca aaataacaca agaccacagc
      301 tacgaaagtg ccgacagcga tgaggacgat gagcccgatg gtggcggagg aggtgcagcg
      361 gcagacgaaa cactgtttgc tatgctcaaa gacttgtgca aacaaatagc caaacaaaaa
      421 aatctgcctc cttttgtaat attccaagag ccttcgctcg aagaaatggc catacagtac
      481 cccatcaaca tggaggaaat gaaaaacatt accggtgtgg gtgcaggtaa ggcaggcaag
      541 tatggaaaac cattcgttga cctcataaaa aaatatgtgg aagaaaacga aattgaacgt
      601 ccgcaagatt tcattgtaaa atctgttgtc aacaaatcag gtctcaaggt ttatcttatt
      661 caaaacatcg accgcaaaat ttctctcgat gatatggccg aagctaaagg catcccttta
      721 ggcgatatca tcacagagtt agaaagcatc gtggcatcag gaacaaaaat tgatttgaat
      781 tattacctca acgaagttat tgatgccgac cgtcagcaag atgtgttcga ctatttccgc
      841 acggcagaaa gtgacagtgc agaagatgcc ttgctcgaac ttggcgaaaa cgactacacc
      901 attgacgaaa taagaatgat gcgcgtaaaa tttatgagtg aattaggaaa ttgattaaaa
      961 ccaaatctat cgttcacaaa aggctgcctc gcaagggcag cctttttttg tgagttttat
     1021 aaaagtgagc atttcaagcc acaattatca attatatttt tccgaatcat ttatttttga
     1081 ggtaaatgaa taatagtgat cggagctata gcttccaaac aaactactat cagaggtgat
     1141 gtcaagaaaa cttgcagtat atacttttaa tatatacctt tgtaaaaaaa gagatatgaa
     1201 aactttatca ttaaagcttg acgataaaat atttaatgaa actgaagaaa ttacttcaaa
     1261 attaaaattg gcccgaaatc gttatatcaa tgaggctgtt agtatttaca atacattaaa
     1321 caaaaggcgt ttattaaaaa aacaacttgc caaagaatca aaattgacga gtaaagactc
     1381 catggacata ttacgtgaat ttgaaaagtt aatggatgaa aattaaacaa tttgatattt
     1441 ggttagctga cctgaatcca agtatcggaa ccgagcccgg caaaaaaaga cctgtggtta
     1501 ttgttcaaac tgacctttta aatgagtcac atctatcaac attaatttgt acaattacaa
     1561 cgaatgtaca aaaagaaatt ctgttattaa gagttcattt aagaaaagga caactcgaaa
     1621 aattaagtga catattggta gatcaggtta gaacaataga caataaaagg ttaattaaaa
     1681 aacttggtca tctgaacaaa gaccaaatac taaaacttaa aactaactta agaattgttc
     1741 ttgacttata gttgcaacct gtaagtgcga agtcactgca ttgtgtgaag agcaacaggt
     1801 catttttatt aactttcctt atcgtgttgc tatcctaaat actgtctcaa cgattcaaca
     1861 tcggaatact ctaaggaaaa aacaactttc aaattttcta aactcgcaaa ctttctaaca
     1921 ttcaaaactt tacccatctt caaattatct ttcttaatca cttttctttt tatttttgag
     1981 caaatgaatt gttttaattt tgatagtatc atgaacaaaa tattatcaaa actattattc
     2041 gtgtaatatg caatcaaacg taggtttagt aatagaagaa agagcggcag atattggcaa
     2101 ttttatggtt ggccgactat taccattccg tcaaaaaaga aaggttggcc cttttgtttt
     2161 tatag
//
LOCUS       DAORQT010000275         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10658_length_2156_cov_4.798667, whole genome shotgun sequence.
ACCESSION   DAORQT010000275 DAORQT010000000
VERSION     DAORQT010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10658_length_2156_cov_4.798667"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PK134_07990"
     CDS             complement(<1..790)
                     /locus_tag="PK134_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38828.1"
                     /translation="MISNLVFIRLQQLKRELTKTGWLYSLILLVITGLLGFLLYHVVQ
                     NSTHSFYLAALMIVSVYTIHSTRNDLQFIRQQLRNPFFILTTEYILLALPVIILLFLK
                     SYFIAGCTIIAGCILIALIVPKNSTHLLFPKLARWIPSAMFEWLSGIRNTKGIVAVFL
                     IVLYATCFLKFIPLIFLWLTTITIAGFYQFGEPLSLLYLHENAKQLLRIKIKNVLLLY
                     VKITLIPVLVNIFLHPDIWWVDLMIYILCMLVIICAVLIKYAVYR"
     gene            complement(810..1370)
                     /locus_tag="PK134_07995"
     CDS             complement(810..1370)
                     /locus_tag="PK134_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRB38829.1"
                     /translation="MKNKIVVAVTGASGSIYASMLLSKLAQLKEQFSDVAIVFSKNAT
                     DVWQHELGNDSYKSLSFKRYDTMDFNAPFASGSAQYNTMIIIPCSMGTMGRIAAGISN
                     DLITRAADVILKERRKLILVTRDMPLNLIHINNMKTITEAGGIICPACPSYYSKPQTI
                     NDVAATVVDRALDLAGFSLNSFRWNE"
     gene            complement(1370..1993)
                     /locus_tag="PK134_08000"
     CDS             complement(1370..1993)
                     /locus_tag="PK134_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRB38830.1"
                     /translation="MFKIINKILIVLLLPMATNAQSFKASIIGGINASQVSGDELGGF
                     NKAGVMFGGSAILPVTPKSDVAMELLFIQKGSKTPTSKDNSNTVYYKMSLNYLEVQIN
                     YTYHASKKIGLHVGPTFGVLVGEKEEDIAGELTERPEFQKTEIGIAGGLSFHFSEKVG
                     LYMRLSNSLLPIRKMGADTKYFKSGQYNSGLAFFLTYTFSSKDKQKQ"
BASE COUNT          707 a          393 c          354 g          702 t
ORIGIN      
        1 gcctgtaaac agcatattta atcagtacag cacaaataat aacaagcata caaagtatat
       61 aaatcattaa gtcaacccac caaatatccg gatggagaaa tatattcact aaaacaggaa
      121 tgagagttat ctttacataa agcaacaaaa cattttttat tttaattctt aaaagctgtt
      181 tggcgttttc atgtaaatag agtagtgaaa gtggttcacc aaattgataa aacccggcaa
      241 ttgttattgt tgtcagccaa agaaatatca atggaataaa ttttaagaaa caggtcgcat
      301 acagtacaat taaaaagact gcgacaatgc ctttagtgtt gcgtatgcca ctcaaccatt
      361 caaacattgc tgatggtatc caccttgcaa gttttggaaa aagtaaatgt gttgaatttt
      421 ttggtactat taaagctatc aatatgcatc ctgcaattat tgtgcatcct gcaataaaat
      481 aactttttaa aaataacaga atgataacag gaagtgctaa caaaatatat tcagttgtca
      541 gaataaaaaa tggatttcta agttgttgtc tgataaactg caaatcattt cttgtactat
      601 gaatagtata gacactaaca atcatcaatg ctgctaaata aaaactatga gtagaatttt
      661 gtactacatg ataaagtaaa aatcctaaaa gtcctgtaat gactaatagt ataagactat
      721 ataaccaccc tgtttttgtc aactcgcgtt taagttgctg cagtctgata aaaacaagat
      781 ttgaaatcat ttgaaattcc gaagactgtt tattcattcc aacggaagct gttcaaacta
      841 aaaccggcaa ggtctaatgc acggtcaact acagttgctg ccacatcatt gatggtttgt
      901 ggcttgctgt aatatgatgg gcatgcaggg caaattatcc ctccggcttc agtaatagtt
      961 ttcatgttgt tgatatgaat cagattcagt ggcatatctc ttgtaactaa gataagtttt
     1021 cttcgctctt ttagtatcac atcggcagca cgtgtaatca ggtcgtttga aattcctgca
     1081 gcaatgcgtc ccattgttcc catgctgcaa ggaataataa tcatagtgtt gtattgtgcc
     1141 gatcccgatg caaaaggtgc attaaaatcc attgtatcat atctcttaaa ggaaagtgat
     1201 ttataggaat cattacctaa ttcatgctgc cacacatctg ttgcattttt tgaaaacact
     1261 attgcaacat cagaaaattg ttcttttagc tgtgccaatt tactaagcaa cattgatgca
     1321 taaatacttc cactggcacc ggtaacggca actacaatct tgtttttcat tattgctttt
     1381 gtttgtcttt tgagctaaaa gtgtaagtaa gaaaaaatgc caatccacta ttatattgtc
     1441 cgctcttaaa atattttgtg tcggcaccca ttttacgaat aggcaacaat gaatttgata
     1501 agcgcatata caaacccact ttttcgctga aatgaaatga tagcccgcct gcaataccta
     1561 tttctgtctt ttggaattcc ggtctttctg tcaactcacc tgctatatct tcttctttct
     1621 caccaaccaa tacaccaaac gtaggtccaa cgtgcaggcc tattttttta gatgcatgat
     1681 aggtataatt aatttgcact tccagataat ttaaactcat cttataatag acggtattgc
     1741 tattgtcttt tgatgttggc gttttacttc ctttctgaat aaacaacaat tccattgcaa
     1801 catctgactt tggtgtgaca ggtaatatgg cactacctcc aaacattact cccgctttat
     1861 taaaacctcc caactcatca ccggaaactt gtgatgcatt gatgccacca atgatggaag
     1921 ctttaaatga ttgcgcattt gtagccattg gcaataaaag cacaatcaga attttattaa
     1981 taattttaaa catagtgcga tgttatgtac aacaaaagta aaaattaaca ggttgtaaag
     2041 gttggttatt tacagttcaa gtgttaatct tgcattaaat aaacagtaaa tatgcgcctt
     2101 gtagttctat tgctttttgt ttttactcag tcggtagtgg cgcagccatt aattga
//
LOCUS       DAORQT010000276         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10745_length_2144_cov_5.285783, whole genome shotgun sequence.
ACCESSION   DAORQT010000276 DAORQT010000000
VERSION     DAORQT010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10745_length_2144_cov_5.285783"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..282
                     /locus_tag="PK134_08005"
     CDS             19..282
                     /locus_tag="PK134_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB38831.1"
                     /translation="MDSRRSLRLSSRSVQQVFKDALKNAKVNKKVGVHGLRHSYATHL
                     IEQGTDIRFVQELLGHSNIKTTMAYTKLTDQIRRKIKSPLDNF"
     gene            complement(444..941)
                     /locus_tag="PK134_08010"
     CDS             complement(444..941)
                     /locus_tag="PK134_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38832.1"
                     /translation="MLHIITKQVSALNGQMLEITHPVVAGVYYITPIVYGNATGTGNI
                     TALTLDPNCTYTGTSVMVEFLAQGVICPTGIKELNGAAFAVTGIYPVPVKNILNIAVE
                     SAKSTTMSISIKDVLGRTVTSSTQSVNNGSNTISIDTQNLSSGVYTLTVNNEKQQSTV
                     KFVKE"
     gene            928..>2144
                     /locus_tag="PK134_08015"
     CDS             928..>2144
                     /locus_tag="PK134_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38833.1"
                     /translation="MMCNINAGGGMIYKTVDGGTTWTQNSIAGMYSQATSFGNVIYFS
                     DSQNGFTMGDPVGTGVNKRYELYTTNDGGDNWTAVPIANIPALTNAGEYGITNLFSAV
                     GSNIWFATTYGDVLHSSDNGLTWTKSASGFPPYTANGNRQDITDIAFSDANNGMIVQV
                     NASAYIVKQTTDGGATWTTVTTLGNFYPTEIEAVPGTSIYVSGGSNTTYGFGSSFSTD
                     NGLTWTPLDTNFSHTTFDFIDASTGYGAEYIAAGSPGGAWKFSGTLSNNPLNDDCAGA
                     TNINAQFGQAPGTLTSSGPYDNTNATTGPQDPTTGFACFGEPNGSGTAPTLDNTLWFT
                     FTGDGNTYFIEAPNTCAGVTDPMDEGDTQIAIYTGTCGNLTPVACNEDGPNANATNSY
                     YPAGLNFTTTPGTV"
BASE COUNT          671 a          410 c          426 g          637 t
ORIGIN      
        1 acctaacaaa aaacttgaat tgacagcagg cggagtttac ggttatcaag tagaagtgta
       61 caacaagtat ttaaagatgc attaaaaaat gctaaggtca ataaaaaagt aggagtgcac
      121 ggtcttcgtc attcctatgc tacacatttg attgagcagg gaacagatat acggtttgta
      181 caggaacttt taggtcatag taatattaaa actacgatgg cttataccaa actgacagat
      241 caaatcaggc gaaaaataaa aagccctttg gataattttt aactgcgtaa ctattgaaga
      301 tttatttgtt tcaggatata tcaaaacatg gttattcaaa acttaaggct cataatttaa
      361 tgtggaataa tgtgtagtat aagttaccca taaaaaaagg ctgccattac gaggcagcct
      421 tttgtagttt caaacaatct gaattattct tttacaaact taacagttga ttgttgtttt
      481 tcattgttta ctgtaagtgt atatacacct gatgaaaggt tttgtgtatc tatagaaatg
      541 gtgttgctgc cattgtttac agattgtgta cttgaagtta cagttcttcc aagtacatct
      601 ttaattgaaa ttgacatcgt tgttgacttt gcagattcaa cagctatgtt caatatgttc
      661 tttacaggta caggataaat tcctgtaact gcaaaagcag caccattaag ttcttttatg
      721 cctgttgggc agataactcc ctgtgctaag aattcaacca taacggaagt acctgtatag
      781 gtacagttag ggtcaagagt aagcgctgta atatttcccg ttcctgttgc attaccataa
      841 actattggag tgatgtagta aacacctgca accacaggat gggttatctc caacatctgg
      901 ccattaagtg ccgacacttg ctttgtgatg atgtgcaaca tcaatgcagg cggtggaatg
      961 atttacaaaa ctgttgacgg tggtaccaca tggacacaaa attcaattgc cggtatgtat
     1021 tctcaggcaa catcttttgg aaacgtaatt tatttttcgg attcacagaa tggatttact
     1081 atgggcgacc ctgttggtac aggcgttaac aaacgttacg aactttatac taccaacgat
     1141 ggtggtgata actggacagc agtgcctata gccaacattc ctgcattaac taatgcgggt
     1201 gaatatggta tcaccaactt gttttcagca gttggaagca acatttggtt tgcaactacc
     1261 tatggtgatg tactccattc atcagacaat ggtttaacat ggacaaaatc tgcttcaggc
     1321 tttcctcctt atacagcaaa cggtaatcgt caggatatta cagacattgc cttcagcgat
     1381 gctaacaacg gaatgattgt tcaggttaat gcatctgcat acattgtaaa acaaacaact
     1441 gatggtggtg ctacatggac tactgtaaca actttaggta atttctaccc aacagaaatt
     1501 gaagccgttc ccggaacatc aatctatgtt agtggtggtt ctaatactac ttatggcttt
     1561 ggttcatctt tcagtacaga caatggcttg acatggacac cacttgacac aaacttctca
     1621 catacaacgt tcgactttat tgatgcatca acaggttacg gtgcagaata tattgcagca
     1681 ggtagcccgg gtggagcatg gaagttttca ggaactctta gcaacaaccc tttaaatgat
     1741 gattgtgcag gtgcaacaaa catcaatgcg caattcggtc aggctcccgg aacattaact
     1801 tcatcaggac cttatgataa cactaatgct actacaggtc cacaggatcc aacaacaggc
     1861 tttgcttgtt ttggagagcc taacggaagt ggtactgcac ctactttaga taacacacta
     1921 tggtttacat tcaccggtga tggaaatacc tattttattg aagcaccaaa tacttgtgca
     1981 ggtgtaaccg atccaatgga cgaaggcgac acacaaattg caatatatac aggtacatgt
     2041 ggcaacctta ctcctgttgc atgtaatgaa gatggtccaa atgcaaatgc tacaaactca
     2101 tattatcctg caggtttaaa ctttactaca acaccgggta cagt
//
LOCUS       DAORQT010000277         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10766_length_2142_cov_4.919023, whole genome shotgun sequence.
ACCESSION   DAORQT010000277 DAORQT010000000
VERSION     DAORQT010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10766_length_2142_cov_4.919023"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1033
                     /locus_tag="PK134_08020"
     CDS             56..1033
                     /locus_tag="PK134_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRB38834.1"
                     /translation="MNVLVYIETAEGKLKKSATEVLSYGKAIAKNSGGMLTALAIGDL
                     AADEQSKAGRYGADKVLHVTNDKLKQFINQAFASVVAEAAKQSNTSVVVISNTFNGKA
                     MAPRVAVKLKAGMVDGAVGLPDLNNGFKVRTSAFSGKAFADIELTSAIKVITVSPNSF
                     KAEENQAAGTIEAFSPSVADSDFTAMVKEIVKASDKISLPEAEIVVSAGRGLKGPENW
                     GMIEELATLLGAATACSKPVSDAHWRPHSEHVGQTGLAISPNLYIAIGISGAIQHLAG
                     VSSSKTIVVINKDPEAPFFKAADYGIVGDAFEVIPKLIEEVKKFKAANG"
     gene            1067..1684
                     /locus_tag="PK134_08025"
     CDS             1067..1684
                     /locus_tag="PK134_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRB38835.1"
                     /translation="MKKIRLDIFGLSYSQAQSGAYALVLNEQGGTRRLPIIIGGYEAQ
                     AIAVELENMTPSRPLTHDLFKNFSISFGIHIDEVTIYNLKEGVFYSKIVCNNGEKQLE
                     IDARTSDAIALAVRFNCPIYTFDFILAQAGVEMDEDLGTATSSEMPKIEKPAPAPRKK
                     TKSIGSNDFSHLSTEELNEALINAIDNEDYEAASKIRDELTRRKK"
     gene            complement(1738..>2142)
                     /locus_tag="PK134_08030"
     CDS             complement(1738..>2142)
                     /locus_tag="PK134_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HRB38836.1"
                     /translation="DYLENTTEGLDKMVNSSNSKLNSMLSNIDGVAGNLNNKQSDING
                     ILKNVNSLSDSLSRLQLNLTIEKLNKTIAELNSVLGKIDNSQGSLGKLVNDDSLYNNL
                     NNASANLDKLLIDLKANPGRYVNISVFGRKNK"
BASE COUNT          688 a          359 c          438 g          657 t
ORIGIN      
        1 gcattacaca cagaagcaaa agttatttaa aaataaaaat ttgagaattt aagatatgaa
       61 tgttcttgta tatatagaaa ctgcagaagg aaaactgaaa aaatcggcaa cggaagtttt
      121 aagttatggt aaggcaattg ccaaaaattc aggtgggatg ttaacggctc ttgctattgg
      181 tgatttggca gctgatgagc aaagcaaagc aggccgctat ggtgctgata aagttttaca
      241 tgttacgaat gataaattaa agcaatttat aaatcaggct tttgcttcag tagttgcaga
      301 agcagcaaaa caaagcaata catctgttgt agtaatttca aatacattta atggaaaggc
      361 tatggcacca cgtgttgccg ttaaacttaa agcaggtatg gttgatggcg ctgttggttt
      421 acctgattta aataatggtt ttaaggtaag aacttcagct ttttcaggca aagcatttgc
      481 tgacattgag ttgacatcgg ccataaaagt aattactgtt tctccaaaca gttttaaagc
      541 agaagaaaat caggctgcag gcacaattga ggcatttagt ccatctgttg ctgacagtga
      601 ttttactgca atggtaaaag agattgtaaa agcatcagat aaaatatcat tacctgaagc
      661 tgagattgta gtttcagcag gtcgtggttt aaaaggaccg gaaaactggg gaatgataga
      721 agaacttgca actttattag gtgctgcaac agcatgttct aagcctgtta gtgatgcaca
      781 ctggcgtccg catagtgagc acgtagggca aacggggtta gctatcagtc ctaatttata
      841 tattgctatt ggcatttcag gtgctattca acatcttgca ggtgtaagtt catcaaaaac
      901 cattgttgtt atcaataaag atccggaagc accattcttt aaagctgccg attatggtat
      961 tgttggtgat gcttttgaag tgataccaaa acttattgaa gaggttaaaa aatttaaagc
     1021 cgctaatggc taatcatatt aaaagtttgt ttacttttaa accttgatga aaaaaataag
     1081 actcgatatt ttcggtttgt cttatagtca ggcacaaagt ggcgcctatg ccttggtatt
     1141 gaatgagcaa ggtggcacac gaaggctacc tataataatt ggcggttatg aagcgcaggc
     1201 tatagcagtt gagttggaaa acatgacacc cagtcgcccg ttgacacacg atctttttaa
     1261 aaatttttca atctccttcg gtattcatat tgatgaagta acaatctaca atctgaaaga
     1321 aggtgttttt tattcaaaga ttgtctgcaa caatggcgaa aagcaacttg aaattgatgc
     1381 ccgcaccagt gatgccatag cgctggctgt aaggttcaat tgtcctattt atacatttga
     1441 ttttatattg gcacaagccg gtgttgaaat ggacgaggat ttaggaacag caacaagcag
     1501 tgaaatgcct aaaatagaaa agcctgcacc tgctccaaga aaaaagacaa aatcaattgg
     1561 cagtaacgat ttttcgcacc tttctacaga ggaattgaac gaggcattga ttaatgccat
     1621 tgacaacgaa gactatgagg ccgcctctaa aatcagagat gaattaacga gaaggaaaaa
     1681 ataaactttt gccaaaagtg agcttcttaa tgcgtgcata tttttataca ctctctccta
     1741 cttatttttc ctaccaaaca cagaaatgtt aacatatctt cccgggtttg cctttaaatc
     1801 tataagaagt ttgtcaagat tagctgatgc attgttaagg ttattgtaaa gactgtcatc
     1861 atttaccaat ttccccaaac taccttgcga gttatctatc ttgcctaaca cactatttag
     1921 ttcggcaata gttttattga gtttttcaat agtgaggttt agctgtaacc tgctgagtga
     1981 atcactgagc gagttaacat tttttaaaat accattgatg tcggattgtt tgttgtttag
     2041 gttacctgcc actccatcaa tatttgaaag catactgttg agtttgctgt ttgaactgtt
     2101 caccatttta tccaaacctt cagttgtatt ttcaaggtaa tc
//
LOCUS       DAORQT010000278         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10783_length_2140_cov_6.432614, whole genome shotgun sequence.
ACCESSION   DAORQT010000278 DAORQT010000000
VERSION     DAORQT010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10783_length_2140_cov_6.432614"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK134_08035"
     CDS             complement(<1..554)
                     /locus_tag="PK134_08035"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRB38837.1"
                     /translation="MIKPTSFGFNIETSKDNVFQKQNNRLSNQQIQLEAEKESRLLRD
                     KLIDSNINVIYAYENCEHNTPDAVFPNNWFSTHDNDTLVLYPMLAANRRNERNAALIN
                     YLKNSFQIKEVVDMTPFENENKFLEGTGSLVFDHKNKICFASISERTNTELANNINKK
                     LGYQTIILNYTDENNTPVYHTNVC"
     gene            complement(584..1576)
                     /gene="gap"
                     /locus_tag="PK134_08040"
     CDS             complement(584..1576)
                     /gene="gap"
                     /locus_tag="PK134_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRB38838.1"
                     /translation="MTRVAINGFGRIGRTVLKKLLLNKNLTIVGINDLTDPKTLAHLF
                     KYDSIHGKFDGTVTSAEKAIIINNQTIPVYAEKDPAMLPWKDLQVDVVIESTGRFTKA
                     ELAEAHIKAGARKVIISAPGSGNLKSVVLGVNENILDGSEKIISNASCTTNNAAPMIK
                     ILQDNWGVESAYITTVHAYTGDQNIHDAPHKDLRRARAAAQSIIPTTTGAAKAVTEIF
                     PDLKGMIGGAGIRVPVIDGSITDITCILKHDATVEAINQKFLEASQGSMKGIIEYTTD
                     PIVSVDIIGNTHSCIFDSMLTSVLGKMVKVVGWYDNEMGYSTRLAELTALVGKQ"
     gene            complement(1589..>1883)
                     /locus_tag="PK134_08045"
     CDS             complement(1589..>1883)
                     /locus_tag="PK134_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRB38839.1"
                     /translation="INAFGLRTKSGVMLGLGETRDEVLETMNDLYQNGVHILTLGQYL
                     QPTKEHLPIAEFVNPAVFKEYKQIGLEMGFKYVESGPLVRSSYHAERHLTFDV"
     assembly_gap    1884..1893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1894..>2140)
                     /locus_tag="PK134_08050"
     CDS             complement(<1894..>2140)
                     /locus_tag="PK134_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRB38840.1"
                     /translation="ITSVDRDDLKDGGSEIWAETIRKVREISEGTTMETLVPDFAGKW
                     ENLERVMQERPEIMSHNLETVRRLTKEVRVQAKYDRSL"
BASE COUNT          587 a          411 c          356 g          776 t
ORIGIN      
        1 atacatacat tagtatgata gactggtgtg ttgttttcat cagtgtaatt cagtattatt
       61 gtttgataac ctaatttttt gttgatatta tttgccaact cagtatttgt tctttctgag
      121 attgaagcaa aacaaatttt gtttttgtga tcaaatacta agctgcctgt tccttctaaa
      181 aatttatttt cattttcgaa tggagtcatg tcaacgacct ctttaatttg aaaagaattc
      241 tttaaataat ttataagtgc agcattccgt tcgtttcttc tgttagcagc aagcattgga
      301 taaagtacca aagtatcatt atcatgagta ctaaaccaat tgttcggaaa cacagcatca
      361 ggtgtgttat gttcgcagtt ttcataggca taaatcacat tgatgttgct atcaattaat
      421 ttatctctta acaaacgact ttccttttca gcttcaagct gtatttgctg attgcttaat
      481 ctgttatttt gtttctgaaa gacattgtct ttgcttgttt ctatgttaaa accaaaagaa
      541 gttggcttta tcataagaac agttgatgca aagtgcatat tgtttattgc ttgccaacta
      601 atgctgtcag ttcagctaaa cgtgtgctgt agcccatttc attatcgtac cagccaacaa
      661 ctttaaccat tttaccaagt acagatgtga gcattgaatc aaaaatgcat gaatgtgtat
      721 tgccgataat gtcaacagac actattgggt cggttgtgta ttcaataatg cctttcatag
      781 agccttgcga tgcttccaaa aatttttgat ttatagcttc aacagtggca tcgtgtttaa
      841 gaatgcaggt gatatctgtt attgaaccat ctattaccgg aactctgatt cctgcaccac
      901 caatcatacc tttaaggtca ggaaaaattt ctgtcactgc cttagcagca cctgttgttg
      961 tgggaataat ggattgtgca gcagcacgtg cccttcgtaa atctttatgt ggagcatcgt
     1021 gtatattttg atcgcctgtg taagcatgca ctgtagtaat gtaagcactt tctactcccc
     1081 aattgtcctg aagtattttt atcattggtg ctgcattgtt tgtggtgcaa cttgcattgg
     1141 aaattatttt ctcacttccg tcaagtatgt tttcattcac gcccaacaca acagacttta
     1201 aatttcctga tccgggtgca gaaattatta cttttcgtgc gcctgctttt atatgggctt
     1261 ctgccaattc agcttttgtg aaacgccctg ttgattcaat cactacatct acctgtaaat
     1321 ctttccatgg caacattgca ggatcttttt ctgcataaac aggaatagtt tgattattga
     1381 tgatgattgc tttttctgca gaagtaacag ttccatcaaa cttgccatgg atactatcgt
     1441 atttaaataa atgagctaac gtttttgggt ctgtcaaatc attgatgcct acaatagtca
     1501 ggtttttatt cagaagtaat ttttttaaaa ctgtacggcc aattcttcca aagccgttta
     1561 ttgcaacacg tgtcataatt tattataatt aaacatcaaa agttaaatgt ctttctgcat
     1621 gatagctgga gcgtactagc ggcccgcttt caacatattt aaaacccatt tcaaggccta
     1681 tttgtttgta ttctttaaaa acagcaggat taacaaattc tgcaataggt aaatgttcct
     1741 tggttggttg aaggtattgt cctaaagtta aaatatgaac tccgttttga tataagtcat
     1801 tcatagtttc taaaacttca tcacgtgttt cgcctaaacc taacatcaca cctgattttg
     1861 ttcttaatcc aaaagcattg attnnnnnnn nnnaagactt ctgtcgtatt ttgcctgaac
     1921 acgtacttct tttgtaagtc ttcttactgt ctccaaattg tgcgacataa tctcaggtct
     1981 ttcctgcatc actcgctcta aattttccca ttttccggca aagtccggaa ccaatgtttc
     2041 cattgttgtt ccttctgaaa tttctctaac ctttctgatg gtttctgccc aaatttcaga
     2101 accgccatct ttcaaatcat cacggtcaac actggtgatt
//
LOCUS       DAORQT010000279         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_10959_length_2118_cov_3.858943, whole genome shotgun sequence.
ACCESSION   DAORQT010000279 DAORQT010000000
VERSION     DAORQT010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10959_length_2118_cov_3.858943"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>439)
                     /locus_tag="PK134_08055"
     CDS             complement(<1..>439)
                     /locus_tag="PK134_08055"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38841.1"
                     /translation="NFGNGVTSTLANPSYTYNNASNYTVTLIATNASGCSDTLVFPSQ
                     IIVNQTPRARFNMSPATGCSPLTVNFTNTSANTSAATYSWNFGNGNTASTANTSSVYP
                     DSGTFAVTLLVTNGNGCSDDSVRNVVVNLSPTASATTNDTTGCN"
     assembly_gap    440..449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<450..1992)
                     /locus_tag="PK134_08060"
     CDS             complement(<450..1992)
                     /locus_tag="PK134_08060"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38842.1"
                     /translation="MIRINRAAPNFSFTQSSQCFPITVTLTDLTGPTPAIQWGWNFYN
                     GSTDSTQNPVYTFNAPPTSDTISLWVRDTNGCSATKRLKGIKYKQAKFTMSATGGCKP
                     STICFTDASDTTVVSRKWDFGNGATSTQTNPCYTYTTDGVYTVTLITTFSTGCKDTLV
                     MPNAVTISTPVADFSVPTQAFCAPALVNFNNLSTNATTYYWDFGDGSNSIATTPSHVY
                     NVPGDYTITLIAINGTCRDTMKKVNYLHVPGTFSYFNLVSLQSCVGNLVQFTDSSLNA
                     QTWLWNFGDGDSSTLQNPTHLYSTTGSFTVSLITSDNLGCSSHYIFSNPIVTVGCSPL
                     TSTFTQNSINATSYLWNFGDPASGALNTDTAPTSTHTYNNAGNYQPYFVAYNNFGCSD
                     TMWLPKTINVNQTPVAQFSSSATNGCTPLTVNFTNTSTKLNTPQYLWNFGNGNTAITS
                     NATAVYADSGNFNVSLIVTNAAGCADTATAQITTVLSPTAIASTTDTVGCSPHTVNFT
                     NISLNA"
BASE COUNT          641 a          352 c          458 g          657 t
ORIGIN      
        1 attacatcct gttgtatcgt ttgtagttgc acttgccgta ggcgataaat tgacaactac
       61 atttcttaca gagtcatctg aacatccgtt gccattagtc actaaaagtg taactgcaaa
      121 tgttcctgaa tcaggataaa cagaagaagt atttgcagta gatgctgtat taccattacc
      181 aaagttccaa ctgtatgttg ctgcggaagt gtttgcagaa gtatttgtga agtttactgt
      241 aagtggtgaa caacctgttg caggactcat attgaaccga gctctcggag tttggttaac
      301 aataatctgc gatggaaaaa caagcgtatc actgcatcct gatgcattgg ttgcaattaa
      361 tgttacagtg tagttacttg cattattgta ggtataactt ggattggcaa gtgttgaagt
      421 aacaccattg ccaaagttcn nnnnnnnnnt tgcattaaga gaaatgtttg tgaagttaac
      481 agtatgtgga ctacatccaa cagtatctgt tgttgaagca atagccgttg gcgaaagaac
      541 agttgtaatt tgtgcagtgg ctgtgtcggc acatcctgca gcattagtaa ctataagact
      601 aacattaaag tttcccgaat cggcataaac tgcagtggca tttgaagtaa tggctgtatt
      661 accatttcca aaattccaca aatattgagg agtatttaat ttagttgatg tatttgtaaa
      721 gtttacagtc aaaggtgtac aaccattggt tgcagacgaa ctgaactgtg ctaccggtgt
      781 ctgattgaca ttaattgtct taggcaacca catagtatca ctacatccaa aattattata
      841 tgcaacaaag taaggttgat aattaccggc attattatag gtatgcgttg aagtaggtgc
      901 agtgtctgtg tttaacgcac cagaagcagg gtcaccaaag ttccagagat aagatgtagc
      961 atttattgag ttttgcgtaa atgttgatgt tagaggacta caaccaacag taactatcgg
     1021 attactgaat atataatgtg aagaacatcc aaggttatca cttgtaatca atgaaactgt
     1081 aaagctacct gttgtagaat ataagtgtgt tggattttgt aatgtagaac tgtcaccatc
     1141 accaaagttc cataaccatg tttgtgcatt caatgaactg tctgtaaact gaactaagtt
     1201 tcctacacaa ctttgcagac ttactaagtt gaaataagag aaagttcctg gtacatgcag
     1261 ataatttact tttttcattg tatcgcgaca agttccattg atggcaatta aagtaatggt
     1321 ataatcacca ggaacgttgt acacgtgaga tggagtcgta gcaatagaat ttgagccatc
     1381 accaaaatcc caatagtatg ttgttgcgtt tgtggagaga ttgttaaagt tcacgagtgc
     1441 cggtgcacag aatgcctgtg taggaacact gaaatctgct actggtgttg atatggttac
     1501 agcattcggc atgactaatg tgtctttgca acctgtggag aaagttgtta tcaacgttac
     1561 agtatataca ccatcagttg tataggtata acaaggatta gtttgagttg acgtggcacc
     1621 attaccaaaa tcccatttcc ttgaaacaac tgttgtatca ctggcatcag taaaacagat
     1681 tgtacttggt ttacatccac cggttgccga catagtaaac ttagcttgtt tgtacttaat
     1741 ccctttaagt cgtttggtcg ctgagcatcc atttgtatct ctgacccata atgaaattgt
     1801 atcagatgtt ggtggcgcat tgaatgtgta aaccggattt tgcgttgaat ctgttgaacc
     1861 gttataaaaa ttccaacccc attgaatagc aggagtagga cctgtaagat cagttaatgt
     1921 aacagtaatt gggaagcatt gagaactctg tgtgaatgag aaattcggtg cagctctgtt
     1981 aattctaatc aaggccgggt aagtaactgt acgtgaacag gcaccattgg ttgcagttag
     2041 cgaaatggta tatgcttttg gcgtagtaaa atttcttgat ggatttacta gtgaagaaaa
     2101 tcctgttgat gatgttgg
//
LOCUS       DAORQT010000280         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11005_length_2112_cov_4.945552, whole genome shotgun sequence.
ACCESSION   DAORQT010000280 DAORQT010000000
VERSION     DAORQT010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11005_length_2112_cov_4.945552"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PK134_08065"
     CDS             complement(<1..365)
                     /locus_tag="PK134_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38843.1"
                     /translation="MKDEIYNELKDKAPLLSSIEKKHLFDTPENYFDQLPSAIQQKIK
                     QPQTSTIKTLFSRLLKPQFAIGLSVILLVGGFYLFNQHKADNQYAFVAVNVDSLNVNE
                     LSDLLDTDQLESIAPADNNA"
     gene            complement(396..941)
                     /locus_tag="PK134_08070"
     CDS             complement(396..941)
                     /locus_tag="PK134_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB38844.1"
                     /translation="MNHYSDKELLEQFSNGNADYAFNLIVRKYQQKIYTHVRRLVADH
                     DDADDLTQTTFIKAWKALGNFRADAQLYTWLYRIATNECYTFLKNKRKRFFIPIHDVE
                     AELADKLEHEPSLKANELELKLQKAILSLPEKQRAVFNLRYYDEMKYEDMSEVLGTSV
                     GALKASYHHAVQKIEKFLQSF"
     gene            complement(1006..1974)
                     /locus_tag="PK134_08075"
     CDS             complement(1006..1974)
                     /locus_tag="PK134_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB38845.1"
                     /translation="MKKFTYTEKKDTRSGFGAGLLEAGKANPNVVALCADLTGSLKMD
                     AFEKAFPERFFQCGIAEANMMGIAAGMTIGGKIPFTGTFANFSTGRVYDQIRQSIAYS
                     GKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVINPCDYNQTKAATMAIAKH
                     FGPVYLRFGLPSWPIFIPENTPFEIGKALLLSEGSDVSIFATGHLVWKAIEAGMALAD
                     MGISAEIINIHTIKPLDEKAVIQSVSKTGCAITAEEHQINGGLGDSICQLLSRHLPSP
                     VEMVGVKDTFGESGTPDALMTKYGLDSKNIVFAVEQVLQRKKELAG"
BASE COUNT          605 a          413 c          371 g          723 t
ORIGIN      
        1 gcattgttat ctgccggtgc aatagattct aactgatcgg tatctaacaa gtcactcagt
       61 tcattgacat ttaaagaatc aacatttact gcaacaaatg catattgatt atctgctttg
      121 tgttgattga ataaataaaa acctccaacc aaaagtataa cagacaatcc aattgcaaat
      181 tgcggtttca gcaaccttga gaataaagtt ttaattgtgc tggtttgcgg ctgtttaatt
      241 ttttgttgaa tggctgaggg cagttgatca aaataatttt caggagtatc aaaaagatgc
      301 ttcttttcta ttgacgacag caacggtgct ttatccttca attcattgta aatttcatct
      361 ttcatactac tttgatgttt ttgatttaaa aaggtttaaa atgattgtaa gaatttttca
      421 attttttgca ctgcatgatg gtaagatgct ttaagagcgc caacagatgt acctaaaacc
      481 tcgctcatgt cttcgtattt catttcatca taatacctga ggttaaatac tgccctttgt
      541 ttttccggta aagataatat tgctttttga agtttaagct ccaactcatt ggcttttaaa
      601 gacggttcat gttctaattt atctgccagt tcagcttcaa catcatggat gggaataaaa
      661 aaccgtttcc ttttgttttt cagaaaagtg taacattcat tggtagcaat tctatacagc
      721 catgtataaa gctgtgcatc ggctctgaaa tttcctaagg ctttccatgc tttaataaaa
      781 gttgtttgcg ttaaatcatc ggcatcatca tggtcagcaa ccaaacgcct tacgtgagtg
      841 taaatcttct gctgatattt tctgacaatg agattaaatg cgtaatcggc attgccgtta
      901 ctaaattgct caagtagttc tttatccgag tagtgattca atgaaaaacc gatttatcct
      961 attatttcaa tttaaaaaga ttcccggtgt ttcacaggga aaaaattagc ctgccaattc
     1021 ctttttgcgc tgcaaaacct gttcaactgc aaagacaata ttcttactgt cgagtccgta
     1081 tttagtcatc agcgcatcag gtgttccact ttcaccgaag gtgtctttta ctccaaccat
     1141 ttcaacaggt gagggcaaat gacgcgacaa taactgacaa atactatctc caagccctcc
     1201 gttaatctga tgttcttctg cagttattgc acatccggtt tttgaaactg attgtatgac
     1261 tgctttttca tcaagaggtt taatggtgtg aatatttatt atttctgcac taatgcccat
     1321 gtcggcaagt gccattcctg cttcaatagc cttccagacc aaatgccctg ttgcaaaaat
     1381 actgacgtct gaaccttcgc ttaggagcaa tgcttttcct atttcaaaag gtgtattctc
     1441 aggaataaat atcggccagg atggtagtcc gaaacgtaaa taaacaggtc cgaaatgttt
     1501 tgcaatagcc atggtggctg cttttgtttg attatagtcg catgggttaa ttacagtcat
     1561 gcccggcaac attttcatca ggccaatgtc ttcaagaatc tgatgtgttg caccatcttc
     1621 gcctaatgtt aaacctgcat gagatgcaca tatctttaca ttcttgccag aataggcaat
     1681 tgattgcctt atctgatcat aaacccttcc agtagaaaaa ttagcaaacg ttcctgtaaa
     1741 aggaattttt ccaccaatag tcatacctgc tgcaataccc atcatgttag cttctgcaat
     1801 gccacattga aaaaaacgct cgggaaatgc tttctcaaat gcatccattt ttaatgaacc
     1861 tgtaaggtca gcacataatg ctactacatt agggttggcc ttgcctgctt ctaacaatcc
     1921 tgcgccaaag ccggaacggg tatctttttt ttcggtgtag gtaaattttt tcattactca
     1981 attatttgtt gatgtttact gtaattcctg atccggaagt tatacggaat gttttttcta
     2041 tagtataaat tgcttcttta gatatttttg gtctgaatac tactctgtaa tttccgggtt
     2101 gtaataatat ac
//
LOCUS       DAORQT010000281         2107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11043_length_2107_cov_9.149610, whole genome shotgun sequence.
ACCESSION   DAORQT010000281 DAORQT010000000
VERSION     DAORQT010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11043_length_2107_cov_9.149610"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PK134_08080"
     CDS             <1..1614
                     /locus_tag="PK134_08080"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HRB38846.1"
                     /translation="ARYITPYGNTFNSTFKSSWTLDVTDFASFLHDSVEIEYIHTGYE
                     TNVGRGWLVSVDFALIEGTPVMNPIAFKRLWHGSFPYGNASNPIENYLGADTVTLNFN
                     TANLRLNVTQSGHGADANYCAEFCSKTRTIYFDNSLVDTKQVWRLCGTNPIYPQGGTW
                     VYDRANWCPGNWVYPAVNNFAVAGGSTHIIDMNMQNYNVTSPSANYVIEGQLFEFGPL
                     NNNIDAAIDEILAPTDKFEFSRSNPTCNNPKIRIKNNGSTAITSATIKYGLVGEPEST
                     FNWTGSINTFATADVDLPGLISYTSGNRIFKAYIADVNNTADQYVYDDTAYSNAYVPE
                     VMDTVFILLVKTNNYPTENGYKLYDENNNIIVQRLPGSMAANTIYRDTIHATPGCYRY
                     EITDSGDDGLYWWANSAQGTGYHRILRLNNSIARNFERDFGSRYSYRFVSAPGAVVGI
                     NNQERYADIVVYPNPTKGIINADITLTSASPLEVSVYNPLGQRVAVQNRTNFNAGIIT
                     FDLENQPKGVYLVKVVANDQVKVQRVVLE"
     gene            1714..>2107
                     /locus_tag="PK134_08085"
     CDS             1714..>2107
                     /locus_tag="PK134_08085"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRB38847.1"
                     /translation="MKRCNQFSLTLFLMLFALFANAQKHKTLDQFTDIDLRGSLNAVY
                     KLDSTWSIEVPEDNVTTQWSLENKTLVLSGAGSNIIVRSPDLQKINISGSGSISAEDT
                     MELHDLNIDISGSGKFKMNYVKAKHIEAN"
BASE COUNT          682 a          385 c          423 g          617 t
ORIGIN      
        1 gcacgttata taacacctta tggcaataca ttcaactcta cattcaagtc gtcatggacg
       61 cttgatgtaa cagattttgc atcattcctt cacgactctg tagagataga atatatccac
      121 accggctacg aaaccaatgt gggtcgcgga tggctcgtta gtgttgattt tgcattgata
      181 gaaggtactc cggtgatgaa tcctattgca tttaaaagat tgtggcatgg cagttttcct
      241 tatggcaatg catccaatcc aattgaaaac tatctcggtg ccgacactgt gacgcttaat
      301 tttaatactg caaatttaag actgaatgtt acccaatccg gacatggtgc cgatgccaat
      361 tattgtgcag aattttgctc aaaaacaaga actatttatt ttgataattc attggtagat
      421 accaagcaag tttggcggtt gtgtggaaca aaccctattt atccgcaagg aggcacatgg
      481 gtttacgacc gtgcaaattg gtgtcccggt aactgggtct atcctgctgt caataatttt
      541 gctgttgccg gaggaagcac gcacattatt gacatgaata tgcagaacta taacgtaaca
      601 agcccttccg ctaattacgt catcgaagga cagctttttg aatttggtcc attgaacaac
      661 aatatagatg ctgccataga tgaaattctt gcacctaccg ataagtttga attttcgcgc
      721 agcaacccta cctgcaacaa tccaaaaatc agaataaaaa acaatggctc aactgcaata
      781 acttctgcaa caataaaata tggattagta ggcgaacccg aaagtacatt caactggaca
      841 ggctcaataa atacatttgc aacagcagat gttgacttac ccggtttaat cagctataca
      901 agtggcaaca gaatttttaa agcatacatt gctgacgtta acaatacggc agatcagtat
      961 gtttatgatg atacagctta ttcaaatgcc tatgttcctg aagtgatgga cactgtattt
     1021 atcttattgg taaaaacaaa taactatcct acggaaaatg gctataaact ttatgatgaa
     1081 aataataata tcatcgtaca acgactaccg ggctcaatgg ctgccaacac aatttatcgt
     1141 gacaccattc atgcaactcc cggttgttat cgttatgaaa taacagatag tggcgatgat
     1201 ggtttatact ggtgggcaaa ctcagcacag ggaacaggtt accatcgtat tctgcgattg
     1261 aacaatagca ttgcaagaaa tttcgaaaga gattttggct cacgttacag ctatcgtttt
     1321 gtttctgctc cgggtgccgt tgttggcatc aacaatcagg agcgctatgc tgatatagta
     1381 gtttatccta atccaaccaa aggaatcatc aatgcagata ttacattaac ttcggcaagc
     1441 ccgttagagg tgtctgttta taatccttta ggtcagcgtg tggctgttca aaacagaaca
     1501 aattttaatg caggaataat aactttcgat ctggagaatc aaccaaaggg agtttatttg
     1561 gttaaagttg ttgctaacga tcaggttaaa gtgcaacgtg ttgtactgga gtaaataaca
     1621 atgaaattta attttacagc aggtagttta gttatataag cacctgctgt aacttttagt
     1681 aacaggcctg cgtcatacaa taaaatgaag gatatgaaaa gatgtaatca atttagtctg
     1741 accttgtttt taatgttgtt tgcacttttt gcaaatgcac aaaaacataa aactttagat
     1801 cagtttacag acatagactt gcgtggatcg cttaatgcag tttataaatt ggacagtaca
     1861 tggtcaattg aagtgcctga agataatgtc accacacagt ggagtcttga aaataaaacc
     1921 cttgtgctaa gtggtgcagg aagtaatata attgtcagaa gtcctgattt gcaaaagata
     1981 aatatttccg gttcgggaag tatttctgca gaagatacta tggaattgca cgacttgaat
     2041 atagatattt caggtagtgg aaaatttaag atgaactatg taaaagcaaa gcacattgag
     2101 gcaaaca
//
LOCUS       DAORQT010000282         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11159_length_2092_cov_6.842415, whole genome shotgun sequence.
ACCESSION   DAORQT010000282 DAORQT010000000
VERSION     DAORQT010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11159_length_2092_cov_6.842415"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK134_08090"
     CDS             <1..198
                     /locus_tag="PK134_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38848.1"
                     /translation="WTVLFRIVFFLIFCFAINYFLPAIENKILDMIHRSVIVTVVYLL
                     LIYRYKVLEEFHHLLPWKRKK"
     gene            complement(199..1299)
                     /gene="ychF"
                     /locus_tag="PK134_08095"
     CDS             complement(199..1299)
                     /gene="ychF"
                     /locus_tag="PK134_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRB38849.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNIGVI
                     TVPDDRLNKLETLVKPQRVVPTTVEIVDIAGLVKGASKGEGLGNQFLGNIRETDAIIH
                     VLRCFDDPNVVHVDGSVNPVRDKETIDFELQIKDLEAIDKKIQRIEKIARVGNDKDAK
                     KAYDVLQVFKKHLESGSAARSAPVQSEDFQYVADIFLLTAKPVLYVCNVDEKSVLTGN
                     KHVDAVREKVKDEKAEVLVIAAAIEAEITQLETFEERQMFLSDLGLTESGVAKLIKAA
                     YRLLQLQTYFTAGEKEVRAWTITQGMTAPQAAGVIHTDFEKGFIRAEVIRYNDFVHFG
                     SEQACKENGKLSVEGKEYVVNDGDVMHFRFNV"
     gene            complement(1369..>2092)
                     /locus_tag="PK134_08100"
     CDS             complement(1369..>2092)
                     /locus_tag="PK134_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRB38850.1"
                     /translation="GKMDEKMDPVLSTLNGGGKLTTQTVSLNNFEPINKLADALKMSQ
                     YKQMNLSNVNLSFKFKNGSVFVEPFETSLAGSKAVISGQSGFDQSINYNIDLSIPKAL
                     MGSAATGVVNGMFASINKATGASFSMPDPVKVKVNMGGTVQKPEIKTSLADAGKGLVE
                     GVKDKAMEQLDAKKQELEDKAKAEADRLKKEAEEKVKSEADRLKKEAEAKAKAEADRL
                     KKEAEERAKKEAKDKLNNIFGK"
BASE COUNT          553 a          482 c          349 g          708 t
ORIGIN      
        1 tggacggttt tgtttagaat agtttttttc ttaatatttt gttttgcaat caattatttt
       61 ctgccagcaa ttgaaaacaa aattcttgat atgattcatc gttctgtaat tgtaacagtg
      121 gtttatctgc tactaatcta tcgttataag gtgcttgaag agtttcatca cctgttgccc
      181 tggaaaagaa aaaaataatc agacgttaaa gcggaagtgc attacatcgc catcattcac
      241 cacatactct tttccttcta cagacagttt tccattttct ttgcaagcct gttccgagcc
      301 aaagtggaca aagtcattat aacggataac ctctgcacga ataaatcctt tttcaaaatc
      361 ggtgtgaata actccggcag cctgtggcgc tgtcataccc tgtgtgatgg tccatgcacg
      421 aacttctttt tcacctgccg taaaatacgt ttgtagttgc agcaagcgat atgctgcttt
      481 aattaattta gcaacacctg actctgtaag tcctaaatca gacaaaaaca tctgacgctc
      541 ttcaaaagtt tctaattgtg taatctctgc ttcaatagct gctgcaataa caagcacttc
      601 ggctttttca tcttttacct tttcgcgcac ggcatctaca tgtttgtttc cggttaggac
      661 agatttttcg tccacattac aaacatacag tacaggcttt gctgtgagta aaaatatatc
      721 agccacatat tgaaaatctt cggactgcac aggtgcactg cgtgctgcac tgccgctttc
      781 taaatgtttt ttaaaaacct gcaacacatc ataggccttc tttgcatctt tatcattgcc
      841 cacacgtgca attttttcaa tgcgttgaat ttttttatca atagcttcaa ggtcttttat
      901 ctgcagttca aagtcaatgg tttctttatc gcgcacagga ttcacagagc cgtcaacgtg
      961 caccacattg ggatcatcaa aacaacgtaa aacatgaatg atagcatctg tctcgcggat
     1021 gtttccaaga aactgattac ctaaaccctc tcccttgctt gcacctttca ccaatcctgc
     1081 aatgtcaaca atttcgacag tagtaggaac aacacgttgt ggctttacca aagtttccaa
     1141 cttattcagt ctgtcgtcag gaactgtaat gacaccaatg tttggctcta tggtgcagaa
     1201 tggaaaattt gcagcctgtg ctttggcatt acttaaacaa ttaaacagtg ttgatttacc
     1261 tacattcggc agcccaacaa taccacattt caatcccata gtaaaatttt tgagggtgca
     1321 aagataacac ttttaccctc tgaaaaatga tttaagtact acagaaattt acttaccgaa
     1381 gatattattc agtttgtctt tggcttcttt tttagctcgc tcctctgctt ctttcttcaa
     1441 acggtctgct tctgccttag cttttgcttc agcttctttt tttagtctgt ctgcttcgga
     1501 cttcactttc tcttctgcct ccttcttcaa acggtcggcc tctgcttttg ccttgtcttc
     1561 cagttcctgc tttttagcat ccaactgctc cattgcttta tctttaaccc cttcaaccaa
     1621 acctttacct gcatcggcaa gcgatgtttt aatttcaggt ttttgaaccg tgccacccat
     1681 atttacttta acttttacag ggtcgggcat actgaacgat gcacctgtag ctttgttgat
     1741 actagcaaac atgccattca ccacacctgt agctgcactt cccataagtg ccttgggaat
     1801 gctcaggtca atattatagt taatgctttg gtcaaaaccc gattgcccac tgatgacagc
     1861 cttgctgcct gcaagtgaag tttcaaaagg ttcaacaaaa acacttccgt ttttaaattt
     1921 aaacgacagg ttcacgtttg aaagattcat ctgtttgtat tgcgacatct tcaacgcatc
     1981 tgctaattta tttatgggtt caaaattgtt cagagaaaca gtttgtgtag ttagctttcc
     2041 tccaccatta agtgtagata aaacaggatc cattttctca tccatctttc cg
//
LOCUS       DAORQT010000283         2081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11249_length_2081_cov_15.868213, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000283 DAORQT010000000
VERSION     DAORQT010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11249_length_2081_cov_15.868213"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="PK134_08105"
     CDS             <1..1701
                     /locus_tag="PK134_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRB38851.1"
                     /translation="AGHYIGEDRFVVRKKITKDLEASGHLVKTEDYKNKVGYSERTDA
                     VIEPRLSLQWFVNMPEISKPALEAVVNGNIKLIPDKFISTYRYWMENVKDWCISRQLW
                     WGQRIPAWYDESGNFVVAKTSQEALAKFAEKNIKTNASAIKQDDDVLDTWFSSWLWPI
                     SVFDGFKDPENKDIKYYYPTNDLVTAPEILFFWVARMIIAGLEYRNDIPFERVYLTGI
                     VRDKLGRKMSKSLGNSPDPLELIEKYGADGVRVGMLLSSPAGNDLLFDESYCEQGRNF
                     ANKMWNAFRLIKNWQPDTSLRQSETGRIATAWMKSKLNEQLALINDSYDKMRISEVLM
                     IKYKLFWDDFCAWYLEMIKPAYGSVAVDTETYNTTIDIFENLLQILHPFMPFLTEELW
                     HELKNRNNRDCIIVSTWPKQSSPDNAILEGMTHAQNIISTLRTFRNEKGISPKTQLQL
                     LTKDKHQTTFDDIILKLANVSSIDKAESFDKGFAFMAGTQEYAIPFDQHIDTAAERLR
                     IEKEIAYNKGFLDTVMKKLGNANFVSKAKPEVIASEEKKKSDAEQKIKSLEQQLNMMS
                     "
     gene            1708..1923
                     /locus_tag="PK134_08110"
     CDS             1708..1923
                     /locus_tag="PK134_08110"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRB38852.1"
                     /translation="MDKPAEWVKVYTTTQPHKVEIVRAVLEEHDIESMVINKNDSSYP
                     GLIGNIEIYVLDKLEVLARFIIKDNEL"
BASE COUNT          738 a          340 c          428 g          575 t
ORIGIN      
        1 gcagggcatt atattggaga ggacaggttt gttgtaagaa aaaaaattac aaaagacctt
       61 gaagcaagtg gtcatttagt taagacagaa gattataaaa acaaagtagg atacagcgaa
      121 cgtactgatg cagttattga acctcgcctg agtttacaat ggtttgtgaa tatgccggaa
      181 atcagcaagc ctgcattaga agcagtggtt aatggcaaca tcaaacttat tcctgataaa
      241 tttatcagca cctatcgcta ctggatggag aatgttaaag actggtgtat atcacgtcag
      301 ctatggtggg gacaacgtat tcctgcatgg tatgatgaat caggaaactt tgtggtagcc
      361 aaaacttcgc aggaggcatt ggcgaagttt gcagaaaaaa acataaaaac aaatgcatcg
      421 gcaataaaac aggatgatga tgttttagac acatggtttt cttcatggct gtggccaata
      481 agtgtttttg atggatttaa agatcctgag aataaagaca tcaaatacta ctaccctacc
      541 aacgatttgg tgacagcacc ggaaattctt ttcttttggg tggcacgaat gattattgcg
      601 ggattagaat atcgaaatga cattcctttt gagcgtgttt atcttaccgg aattgttcgt
      661 gataagttag gcagaaaaat gagtaagtca ttaggtaatt cacccgaccc acttgagcta
      721 atagaaaaat atggtgcaga tggtgttcgt gtaggtatgt tgctttcttc acctgccggt
      781 aacgacctat tatttgatga atcatactgt gagcaagggc gcaattttgc caacaaaatg
      841 tggaatgcat ttcgtctgat aaaaaactgg cagcctgaca catcactgcg tcaatccgaa
      901 acaggaagaa ttgcaacagc atggatgaaa tcaaaactca atgaacaatt agcactcatc
      961 aacgacagtt atgataaaat gcgcatcagt gaagtgctta tgattaaata caagttgttt
     1021 tgggatgatt tctgtgcatg gtatttagaa atgattaaac ccgcttacgg aagcgtagcc
     1081 gttgatacag aaacctacaa tacaactatt gatatttttg aaaacctgct acaaatactg
     1141 catccattta tgccattttt aacagaagag ttgtggcatg aacttaaaaa cagaaataac
     1201 agagattgca ttattgtaag tacatggcca aagcaatcat caccggataa tgctatattg
     1261 gaagggatga cacatgcaca aaatataatt tctactttaa gaacttttcg aaacgaaaaa
     1321 gggatatcac caaaaactca gttacagtta ttgacaaaag ataaacatca aacaacgttt
     1381 gatgacatca tacttaaatt agccaatgtc agtagcattg ataaagcaga aagttttgat
     1441 aaaggatttg cttttatggc aggcacacag gaatatgcta tcccttttga tcagcatatt
     1501 gatactgctg cagaacgtct ccgaattgaa aaagaaattg catacaacaa aggctttctt
     1561 gatacggtaa tgaaaaaatt aggtaatgca aactttgtaa gcaaagccaa gcctgaagta
     1621 attgcaagtg aagagaaaaa gaaatcggat gcagaacaaa aaataaaatc attagagcaa
     1681 caacttaata tgatgagcta actgaatatg gacaagccgg cagaatgggt caaggtatat
     1741 actacaacac aaccacacaa agtagaaatt gtgcgtgctg tgttagaaga gcatgatatt
     1801 gaaagtatgg taatcaataa aaatgattca agttatccgg gattaattgg caatattgaa
     1861 atatatgtac tcgataaact ggaagtgctt gcacggttta ttataaaaga caacgaactt
     1921 tgaataactt ttggacaaga gcatttacgg gtgctgtatt tgtagcagtg atgattgctg
     1981 ccattgaatt cagcccttat acattcatga tacttttcat ggtcatcaac ctgttaagca
     2041 tcattgagtt ttacaaactg tttatccgaa acgaacttac g
//
LOCUS       DAORQT010000284         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11403_length_2065_cov_5.958209, whole genome shotgun sequence.
ACCESSION   DAORQT010000284 DAORQT010000000
VERSION     DAORQT010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11403_length_2065_cov_5.958209"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PK134_08115"
     CDS             complement(<1..1190)
                     /locus_tag="PK134_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRB38853.1"
                     /translation="MRKLLTLSALFVILSGTQSLASSFWTAKSETQEMLSGKRQIVPD
                     KYKVYTLDVQGLKNQLLNAPMEFTMAAKQSPVVITLPMPDGSWNSFSMVESPVMQPKL
                     AAKYPFIKTYSGSGINNQPSWVRIDFTQWGFHALIRTAENDIYIDPYSMQTTSDYLVY
                     FRKDFTKSQSSMVCEFDDLQQGQSANKTLTNSMPSVNRSIGTNLRTYRLALACTGEYA
                     AYYGGTKAGALSGMVTSVNRVTGVYESEVDVRLNLIANTDTLIFLNANTDPYTNNSGG
                     TMLGENQSVVNFFIGTPNYDIGHVFSTGGGGIAYLGCICKTADKAKGVTGSAAPINDP
                     FDIDYVAHEMGHQFGGNHTFNSVTGSCQGNREAGAAYEPGSGVTIMGYAGICGSDDLA
                     AHSIA"
     gene            complement(1272..>2065)
                     /locus_tag="PK134_08120"
     CDS             complement(1272..>2065)
                     /locus_tag="PK134_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38854.1"
                     /translation="ITPLFIFVRQKKIMLKRFVCITISLLAFIIVNAQDNKTEKKDSI
                     EHRILLIPYDPRFYLSDADKDIAEETRKDPKTIRNYFHNRSEWFTSRELKRHYPTISL
                     LQNDTVPAYIQSAGDIFSQTAFDYATPVTRSPESMMNATSDKTLTDSRTATNYLNTDV
                     HNRYMKADIQNKKVLADLSERFNTDIFVFLTQFEIKTNYKNCLDIANKIYEREIRLHF
                     TIYDKTGKLINGNYAIAVIPSNVNQMDEIILKCFPLLAKGIAASL"
BASE COUNT          611 a          396 c          386 g          672 t
ORIGIN      
        1 gctatactgt gtgcagccaa atcatcagag ccacaaattc ctgcataacc cataatggtt
       61 acaccactac cgggctcata agctgcaccg gcttcgcggt taccctgaca acttcctgtt
      121 acgctgttaa aagtgtgatt gcctccaaac tgatgcccca tttcatgagc tacataatca
      181 atatcaaacg ggtcattgat tggtgcagca gatccagtta caccttttgc cttatctgca
      241 gttttacaga tacaacctaa gtaggcaata cctccaccac cggtactaaa aacgtgacca
      301 atatcgtaat tgggagtacc aataaaaaag tttacgactg attgattttc tcccagcatg
      361 gttccgccac tgttattggt ataagggtcg gtgttggcat taagaaaaat taaagtatca
      421 gtgttagcaa ttaaattaag tcttacatct acttccgatt cataaacacc tgtcacacga
      481 tttacagatg taaccatccc tgacaacgct cctgcttttg ttccaccata gtatgcagca
      541 tactcaccgg tgcaggctaa tgccaaacga taggtacgca gatttgtacc tatagaacga
      601 ttcactgaag gcattgagtt tgttaaggtc ttatttgcac tttgaccttg ttgtaagtcg
      661 tcaaactcac aaaccatact gctctgcgac tttgtaaaat cttttctgaa gtaaactaaa
      721 taatcagagg tagtttgcat ggaataaggg tcaatataaa tatcgttctc cgctgttctt
      781 atcaatgcat gaaatcccca ttgtgtaaaa tctatacgaa cccatgaagg ttgattgttt
      841 ataccgctgc ctgagtatgt tttaataaat ggatatttag ctgccagttt gggttgcata
      901 acaggcgact ctaccataga aaaactattc caactaccat cgggcatagg taatgtaatc
      961 acaaccggtg attgttttgc tgccatggta aattccattg gcgcattgag cagttggttt
     1021 ttcagccctt gtacatcaag agtataaacc ttgtatttat caggaacaat ctgacgtttg
     1081 cctgataaca tttcttgagt ttctgactta gcagtccaaa aactacttgc caacgattgt
     1141 gtccctgaca gaattacaaa taatgcgctt agtgttaaaa gttttctcat gtttgaataa
     1201 attgaagttg cgaatgtaaa ctaaaggtag gaaaagatag catccgggta acaaataatt
     1261 aaatttttac ctcataatga tgctgcaatc ccctttgcca acagaggaaa acactttaaa
     1321 ataatttcgt ccatctgatt tacgtttgat ggaataactg caatggcata gtttccattg
     1381 attagctttc cggttttatc atagatggta aaatgaagtc tgatttctcg ctcatatatt
     1441 ttattggcaa tatctaagca gtttttataa ttggttttaa tctcaaattg agtaagaaaa
     1501 acaaaaatat ctgtattaaa tcgctcgctc aaatcggcta atactttttt attctgaata
     1561 tcggctttca tgtagcggtt gtgtacatct gtattcagat aattggttgc tgtacgcgaa
     1621 tcggtcagtg ttttatctga tgttgcattc atcatacttt cgggtgatcg tgtcacaggt
     1681 gtagcatagt caaatgctgt ttggctgaat atatcacctg ctgactgaat ataggcaggc
     1741 accgtatcgt tttgtaaaag tgaaatagta ggataatgtc tttttagttc gcgcgaggta
     1801 aaccattcgc tgcgattgtg aaaataatta cggattgttt tagggtcttt tcgggtttct
     1861 tctgcaatgt ctttatcagc atctgacaaa taaaatcgtg ggtcgtaggg aatgagtaat
     1921 atacgatgtt caatactgtc tttcttttca gtcttattat cctgtgcgtt cacaataata
     1981 aaagccaata gtgatattgt tatacaaaca aatcgtttca acataatttt cttttgtctt
     2041 acaaaaataa agagtggtgt tatgc
//
LOCUS       DAORQT010000285         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11425_length_2063_cov_8.553287, whole genome shotgun sequence.
ACCESSION   DAORQT010000285 DAORQT010000000
VERSION     DAORQT010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11425_length_2063_cov_8.553287"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PK134_08125"
     CDS             <1..496
                     /locus_tag="PK134_08125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB38855.1"
                     /translation="IQHVIPAFKQLLKKYPNALLVLANSHGSYSKQIKKCLEQIPIEN
                     FREIRFEYNLFALYKLFDVFVHVPEYKEYEAFGQVYVEALAAGIPSVFTVSGIAAEFI
                     KNNENALVCNYKNSDDIYNAVVKLLTDDGLRNRLVENGLKVVQTKFNMDRYFSHLKKL
                     YGYD"
     gene            483..1361
                     /locus_tag="PK134_08130"
     CDS             483..1361
                     /locus_tag="PK134_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38856.1"
                     /translation="MGMIEVIANEHTFHVFMFYGDETKNFVKQVSFVKAQYEKNIVLH
                     VAHINSDVEQLSSRIDELKCNFKVVFHVVQKSPFEKVSELVGILRTVEAGWICLFKPG
                     DEWLPYHLSAFRDAMLHFKDFQAYLTLNYLRGYSTIKSVGPEDNIINVKKIELKEQLS
                     LSQLMIHSSLLKSNVFPDFKVDSHFDTLFELILVCSSGMVRINRFTNICTLRYPIHTK
                     AYFDQQTLIVCYLIRNIVKDHRLRFYLLFINWQIGFMGLLKAFRLIGAGVFFKEMLCY
                     YVGLKRGVKKGQIYVD"
     gene            1387..>2063
                     /locus_tag="PK134_08135"
     CDS             1387..>2063
                     /locus_tag="PK134_08135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB38857.1"
                     /translation="MPVYNRADLVKDSIVSVLNQSFKDFELICIDDGSTDNTLQVLKE
                     VSKIDRRIKLISHQNKGRCAARNNGINAASGDWIAFLDSDDYYLENHLSTMYKLVQEF
                     PSFSGIATELVVESKIRDYANCNYYRDQRVFSLSDFAEANPISLNQFCYKKSHYPELL
                     FPDINILTAEDLLFFRMFSFRYKILKSGIITNFVNSHVNRSVNLISTEQFVYWNKFAT
                     NYFIDNFS"
BASE COUNT          645 a          273 c          398 g          747 t
ORIGIN      
        1 aatacagcat gtcattcctg cctttaaaca attattgaaa aagtatccta atgcacttct
       61 tgtattggca aacagtcatg gttcatattc aaagcagatt aaaaaatgtc tggagcagat
      121 accaatagaa aattttcggg aaatcagatt tgagtacaat ttgtttgcat tatataaatt
      181 atttgatgtt tttgttcacg tacctgagta taaggagtac gaagcctttg gtcaggtata
      241 tgtagaagca cttgcagccg gaattccttc tgtttttact gtttccggaa tagcagcaga
      301 gttcatcaag aataatgaaa acgcacttgt ttgtaactat aagaattcag acgatattta
      361 taatgcagtc gttaaattgt tgactgatga cggtcttaga aataggttag ttgaaaatgg
      421 attgaaagta gtgcaaacga aattcaatat ggataggtat tttagccatc ttaagaaact
      481 ttatgggtat gattgaggtg atagctaatg aacatacttt tcatgttttc atgttttatg
      541 gagatgagac gaagaatttt gttaagcaag tttcctttgt aaaggcgcaa tatgaaaaaa
      601 atattgttct tcatgttgct catattaata gcgatgttga acagctaagt agcaggattg
      661 atgaactgaa gtgtaatttt aaagttgttt ttcatgtagt gcaaaaatcg ccttttgaga
      721 aagtcagtga attggttggc atcttaagaa ctgttgaagc aggatggatt tgtcttttta
      781 agccgggtga tgagtggctg ccatatcatt tgtcggcatt tcgtgatgct atgctacatt
      841 ttaaagattt ccaggcatat ttaacattaa attatctgag aggttatagc actataaaat
      901 ctgtaggtcc tgaagataat ataatcaatg ttaagaagat tgagttaaag gagcagttaa
      961 gcctttcaca gttgatgatt catagcagtt tattgaagag taatgtattt cccgatttta
     1021 aagtggattc tcattttgat accctctttg agttgatatt ggtttgtagt tctggtatgg
     1081 tgcgtataaa cagatttaca aatatttgta cattaaggta tccgatacac actaaagcat
     1141 atttcgacca gcaaacatta attgtttgtt atctgattag aaatatagtt aaagatcacc
     1201 gacttagatt ttatctttta tttatcaatt ggcaaattgg gttcatgggc ttgttgaaag
     1261 cttttcgact tattggagcc ggagtttttt ttaaagaaat gctatgttat tatgtaggac
     1321 ttaaaagagg tgttaagaaa ggacaaattt atgttgactg ataagcctcc ctttttttct
     1381 gtcattatgc cggtgtacaa tcgtgcagat ttggttaaag attcaattgt ctctgttctt
     1441 aatcagagtt ttaaagattt tgaactaatt tgtattgatg atggttcaac tgataataca
     1501 ttgcaagttt taaaggaagt ttcaaagata gataggcgta ttaaattgat ttcacatcag
     1561 aataaaggtc gttgtgctgc ccgaaacaat ggaataaatg cagcttccgg tgattggatt
     1621 gcctttcttg actctgatga ttattatctt gagaatcatc tttcaacaat gtataaatta
     1681 gtacaggagt ttccttcctt ttcaggcatt gcaactgaat tggttgttga aagcaaaata
     1741 agagactatg caaattgtaa ttattataga gatcaaagag ttttcagttt gagcgatttt
     1801 gcagaagcta atcctatttc attaaatcag ttttgttata aaaagtcgca ttatcctgaa
     1861 ctactttttc ctgatatcaa tattcttacg gctgaggatt tgttgttctt tcgtatgttc
     1921 agtttcagat ataaaattct gaaatcaggt atcattacaa attttgtaaa ctcacatgtt
     1981 aataggtctg taaatctgat ttccactgaa cagtttgttt attggaataa atttgctaca
     2041 aattatttta ttgataattt ttc
//
LOCUS       DAORQT010000286         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11549_length_2049_cov_5.284353, whole genome shotgun sequence.
ACCESSION   DAORQT010000286 DAORQT010000000
VERSION     DAORQT010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11549_length_2049_cov_5.284353"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK134_08140"
     CDS             <1..440
                     /locus_tag="PK134_08140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRB38858.1"
                     /translation="LKQRESEMNEGMDIALCTINNKNKTLQYSGANRPLWLIRKNEFQ
                     FYKPNKIPIGGQQYNKADGFMQHNITLQQGDAIYLFSDGFVDQFGGEFGKKFMTKKFR
                     ELLLSIQHLSMNEQKEYLLNTLIKWQGKHDQVDDILVIGIKIP"
     gene            444..1778
                     /locus_tag="PK134_08145"
     CDS             444..1778
                     /locus_tag="PK134_08145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38859.1"
                     /translation="MPSKILLVDDEPYIEDLVRQKFRKQIKENQFEFEYVSNGKIALE
                     KLKNDDSYDLMLTDINMPVMDGLTLLEKLRENNILTKAVVVSAYADLSNIRTAMNRGA
                     FDFITKPIDLADLEATIKKTVLEMEMLRKGIEASRNLDDALLQKAEAQQKALDALLEK
                     EKLILQQNELLETQVQQRTAELQQQKLLVEEKNKEILDSIHYASYLQRAILPSEETLA
                     SFFKESFVLYQPKDIVAGDFYWIEPVEDGHIVIVADCTGHGVAGALLSMLGISLLNHL
                     VRDKNITRPAELLNQLRIEVSRSLNQAETHAAEGMDIAVCHINCSAKQITFAGAYRPL
                     VFISGNELKTLSGNKIVIGGNNILQQQEFINQTISFDDAGTVYLFTDGYADQFGGEKN
                     KKLMSGKLKELLLEKHNLPMKEQLTFFTDYLKKWRGKNDQVDDVLLLGFRCQ"
     gene            complement(1786..>2049)
                     /gene="ytxJ"
                     /locus_tag="PK134_08150"
     CDS             complement(1786..>2049)
                     /gene="ytxJ"
                     /locus_tag="PK134_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRB38860.1"
                     /translation="IFKHSTRCSVSTFAKKVLLSEFSDDMEKKFDVYYLDLLNYREVS
                     NKIANHYGVYHESPQILIISDGKCIYDASHDDVSLASALASVN"
BASE COUNT          713 a          371 c          377 g          588 t
ORIGIN      
        1 cactcaaaca acgcgaaagc gagatgaatg aaggtatgga tattgcgctt tgtacaataa
       61 acaacaaaaa caaaacctta caatattccg gtgccaaccg accgctttgg ctcattcgaa
      121 aaaacgaatt tcagttttat aaaccaaaca aaatacccat tggcggacag cagtacaaca
      181 aagctgacgg ttttatgcaa cataacatca cacttcagca aggtgatgct atctaccttt
      241 tttctgatgg ctttgttgat cagtttggtg gagaatttgg taaaaagttt atgacaaaaa
      301 agtttcgtga actgttatta tccattcaac atctgagtat gaatgaacag aaagaatatt
      361 tattgaatac attaattaaa tggcaaggaa aacatgatca ggttgatgac attttagtaa
      421 ttggaataaa aataccctaa cttatgccaa gcaaaatatt attagtagat gacgagcctt
      481 acatagaaga tttagtcaga caaaaattca gaaagcagat taaagaaaat caatttgaat
      541 ttgaatatgt gtctaatggt aaaattgcat tagaaaaatt aaagaatgat gacagctatg
      601 acctgatgct tacagacatc aacatgcctg tgatggatgg gttgacttta cttgaaaaac
      661 tcagagagaa taatattctt actaaagctg ttgttgtttc tgcttatgcc gatttgagca
      721 atatccgtac tgcaatgaat cgtggtgctt ttgattttat taccaaacca attgaccttg
      781 ccgaccttga agcaaccatc aaaaaaactg ttttagagat ggaaatgctt cgaaaaggaa
      841 ttgaagcatc gcgcaacctt gatgatgcat tgcttcagaa agccgaagca caacaaaaag
      901 cattggatgc acttttagaa aaagaaaaat taatacttca gcaaaacgaa cttctcgaaa
      961 ctcaggttca acaacgaact gccgaactgc aacagcaaaa actattggta gaagaaaaaa
     1021 acaaagaaat tcttgactcc attcactatg caagttactt gcaacgcgct atccttccct
     1081 ctgaagaaac attagcatcc ttctttaaag aaagttttgt actgtatcag cctaaagata
     1141 ttgttgctgg tgatttttat tggattgaac ctgttgaaga tgggcacatc gttatagtag
     1201 cagattgtac cggtcacggt gttgcaggtg cattgctgag tatgttggga atatcgctgc
     1261 tcaatcatct tgtcagagat aaaaatatta cccgtcctgc agaattgtta aatcagttgc
     1321 gaattgaagt ttcgcgttcg cttaatcagg cagaaactca tgctgccgaa ggaatggaca
     1381 ttgccgtatg tcatatcaat tgcagtgcca agcaaattac atttgcagga gcttaccgtc
     1441 cgcttgtgtt catttcaggc aatgaactta aaacactttc agggaataaa atagttattg
     1501 gcggcaacaa cattttgcaa caacaggaat ttatcaatca aaccatttca tttgacgatg
     1561 caggcactgt atatcttttt actgatggct atgccgatca gtttggaggc gagaaaaata
     1621 aaaagctcat gtcaggcaaa ctcaaagaac tacttcttga aaaacataac ttacccatga
     1681 aagagcaatt aactttcttt actgactatt taaaaaagtg gcgcggcaaa aacgatcagg
     1741 ttgacgatgt gttgctgctg ggttttcgct gtcaataaat tagtcctaat tgaccgatgc
     1801 caatgctgat gccaatgaaa catcatcatg tgaagcatca taaatacatt tcccgtcaga
     1861 aataattaaa atttgcggtg actcatgata cacaccatag tgatttgcaa ttttattact
     1921 cacctctctg taattgagta aatcgagata atacacatca aattttttct ccatatcatc
     1981 agaaaattct gacaaaagaa cttttttagc aaaagtactt accgaacatc gggtgctatg
     2041 cttaaatat
//
LOCUS       DAORQT010000287         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11680_length_2033_cov_4.631951, whole genome shotgun sequence.
ACCESSION   DAORQT010000287 DAORQT010000000
VERSION     DAORQT010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11680_length_2033_cov_4.631951"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..743)
                     /locus_tag="PK134_08155"
     CDS             complement(69..743)
                     /locus_tag="PK134_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38861.1"
                     /translation="MKFIKYLFCCLIIVHITIAKAEDSIANKKIVLKIAPINFVDQIS
                     HQSAMIGTEYWLGTRYGFEISFAPIFGRDYFNGYGGTGFKSSVEVKRCFSMKGKRLFY
                     LGTKLFYNKINYASKNLFENKITSEEYEEKYFIRKSVYGANITSEVEWRLFPKIILNI
                     NAGIGLRVKNVRHYQRLRPEDNFYSVDVLAENVRDKKGNYVLPDMAIGIKIGYAIDRI
                     KKVQDN"
     gene            complement(1082..>2033)
                     /locus_tag="PK134_08160"
     CDS             complement(1082..>2033)
                     /locus_tag="PK134_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRB38862.1"
                     /translation="IEDRVKFNDLGIVIIDEQHRFGVEQRAKLWMRQQPPHVLVMTAT
                     PIPRTLAMTLYGDLDVSIIDELPAGRKPIKTMHLFESSRLRVIGFMKEQIKLGRQIYI
                     VFPLIEESEKLDLQNLMAGYDSIQRDFPLPEYFVSMVHGKMKAKDKEQEMERFVNGKT
                     HIMIATTVIEVGVNVPNATVMIIENAERFGLSQLHQLRGRVGRGAEQSFCILMSGNKL
                     SADARERLKTMVQTNDGFEIAGTDLKLRGPGDITGTQQSGLLNLKIAELAKDGAILEH
                     ARNAVINILNDDPLLNSEENKIFYRHLHQLREQPNWSKVS"
BASE COUNT          595 a          405 c          317 g          716 t
ORIGIN      
        1 ccaagaagtg caacttcgtg ggtatgggga gggtacgagg gagatgtttt ggaacatgaa
       61 taatgttatc aattatcttg aacctttttt attctatcaa tagcgtaccc gattttaatg
      121 ccaattgcca tatcaggaag aacataattt ccttttttat ccctgacgtt ttctgctaaa
      181 acatcaacac tataaaaatt atcttctggt cgcaatcgtt gataatgtct aacattttta
      241 actcttaaac ctatacctgc attgatattt aaaatgattt ttggaaataa cctccattct
      301 acttcagacg tgatatttgc cccataaact gattttctga taaagtattt ttcttcatat
      361 tcttcagaag taattttatt ttcaaacaga tttttagaag cataatttat tttattgtaa
      421 aacaacttag tgcctaaata aaatagcctt ttacctttca tactaaagca tctttttacc
      481 tcaacagatg atttaaaccc agtgccacca tatccattaa aataatctct tccgaatatt
      541 ggcgcaaaac taatttcaaa accatatcta gtacccaacc agtattctgt tccaatcata
      601 gctgattgat gtgatatttg atcaacaaaa ttaatagggg ctattttgag aactattttt
      661 ttattggcta ttgagtcctc tgctttagca attgttatat gaacaattat caaacagcaa
      721 aacaaatact ttataaattt catttttttc aatttattat tctattagtt ggttatttaa
      781 taaatgttag tgcgaataaa tattcccacc atcgtgcatt tgttattttt ttatttgacg
      841 gaaataatga atatcggtct tttctcccag aatatcttgt acttcgcagg tttttgtgca
      901 aagaagtttt acttctgaaa ccataccgtt tcctttattt ttattgtgta aaaataattt
      961 attgttcact ttgtacagca cagctttatg acacactggc cacgaagtac aaattcgcgg
     1021 ttacggggat atttaaataa aatgagtggc ttcatagatt atcaattcgg taagtgccag
     1081 gctaactcac cttactccag ttaggttgct ctcgcaactg atgcaaatga cgataaaata
     1141 ttttattttc ttctgagttc aacagtgggt catcattcaa aatattgatg actgcatttc
     1201 ttgcatgttc taagatggca ccatcttttg ccaactctgc aattttcagg ttcagtagtc
     1261 cgctttgctg tgttcctgta atatcgccag gaccacgtaa ctttaaatcg gttcctgcaa
     1321 tttcaaatcc atcattggtt tgcaccattg ttttcaatct ttcgcgcgca tcggcagaga
     1381 gtttatttcc cgacattaat atacaaaaag actgctctgc acctctgcct acacgaccac
     1441 gcaactgatg caactgcgac aatccaaaac gttctgcatt ttcaataatc ataacagtgg
     1501 cattaggtac attaacacca acttctataa ccgttgtagc aatcatgata tgtgttttgc
     1561 catttacaaa tcgctccatc tcctgttctt tatcttttgc cttcatcttt ccatgtacca
     1621 tgctcacaaa atactcaggc aatggaaaat ctctttgaat actgtcgtag ccggccatca
     1681 ggttttgcaa gtctaatttt tctgattctt caatcaatgg aaaaacaata taaatctgtc
     1741 ggccaagttt tatttgctct ttcataaatc caataacccg aagtcgtgat gattcaaaaa
     1801 gatgcatggt ttttattggc tttcttcctg caggaagttc atcaattatt gaaacatcaa
     1861 ggtcgccata aagtgtcatg gctaaagtgc gcggaatggg tgtggctgtc atcaccagca
     1921 catgtggagg ttgttgccgc atccagagtt tggcacgttg ctccacacca aaacgatgtt
     1981 gctcatcaat gattactatg ccaaggtcgt tgaattttac tctgtcttct atc
//
LOCUS       DAORQT010000288         2029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11715_length_2029_cov_6.373860, whole genome shotgun sequence.
ACCESSION   DAORQT010000288 DAORQT010000000
VERSION     DAORQT010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_2029_cov_6.373860"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..601)
                     /locus_tag="PK134_08165"
     CDS             complement(5..601)
                     /locus_tag="PK134_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB38863.1"
                     /translation="MWLIRNKDKNALSDEELVLQYRQQGDKQAVGILFNRYSHLVFGV
                     CMKYLKDTDDSKDAVLQIFEKLYADLSKHDVQKFSYWIHRVAQNFCLMQLRSRQAAKN
                     RADNYSRDEIAASTEFEPENTDRVYEKETELLMLEEAIKTLNEEQRICIELFFLKEKC
                     YNEITDLTGFDFKQVKSFIQNGKRNLRIYMEKNNHVKS"
     gene            complement(683..958)
                     /locus_tag="PK134_08170"
     CDS             complement(683..958)
                     /locus_tag="PK134_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38864.1"
                     /translation="MELTIIAKEDLSDYHFVQHEVLSNEIDRARRILLDGAMALGNDY
                     KQKVRIVFETTEGTCAVETTVWAVTNDHVELKAGKDIPIHAIAEVVL"
     gene            complement(1024..>2029)
                     /locus_tag="PK134_08175"
     CDS             complement(1024..>2029)
                     /locus_tag="PK134_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38865.1"
                     /translation="CFCFTSSLQAQQIELKTIQLPVQVSIRALQTVNDSVVWFAANRG
                     VFGFTKDGGSNWHIDSIATDGKYSEFRSLAALNDSTVLLLSIDSPAYLLRTKNYGTSW
                     QHVYSNHSKGIFFDSMTFKDSLHGIAISDPMEGYFMIIKTTDGGNNWQELKVTSPAIK
                     GEGCFAASNTNIQWLQNSVIFATGGSTSRLFISENDGAFLPQTTPMISDSTMTGIFTM
                     YFENKLNGYIGGGNYLQSDTTTTLYQTNNGGKTWQPIKLPEGAFVSCVKTIKTKNGNY
                     LITTGHNGTYVIDSQHYIKEIKNADGLSLAFYTISIAPSQKKVWMAGKSGKMAVLKFY
                     "
BASE COUNT          558 a          413 c          349 g          709 t
ORIGIN      
        1 aattttacga cttaacatga ttatttttct ccatataaat tctgagattc cttttcccat
       61 tctgtataaa actcttcacc tgcttgaaat caaaacctgt taggtctgtt atttcattat
      121 agcatttttc tttcaggaaa aacaattcaa tacatattct ttgttcttcg tttaatgttt
      181 ttattgcttc ttcaagcatc agcaattctg tttctttttc ataaacacgg tcggtgtttt
      241 ccggttcaaa ttctgttgat gcagcaattt catctcggct gtaattgtct gctctgtttt
      301 ttgctgcctg tctgctgcgc aactgcatca gacaaaagtt ttgtgccaca cgatgtatcc
      361 agtacgagaa cttctgtacg tcatgttttg acaaatcggc ataaagtttc tcaaaaatct
      421 gcaacacagc atctttactg tcgtcagtat ctttaagata cttcatgcac acaccaaata
      481 ctaaatgcga atagcggtta aacaatatac ccacagcttg tttatcacct tgctggcgat
      541 attgcaacac aagctcctca tcgctaagcg cattcttatc cttattccgg atgagccaca
      601 taaataattt ttactttctg attaatggta ggatgctttc tggaatatga ttccataaac
      661 attcataaaa ataattttaa aattaaagga ctacctccgc aatggcatga ataggtatat
      721 cttttcctgc ctttaattcc acatggtcat tggtgacagc ccaaacagtt gtttctacag
      781 cacaagtgcc ttcagtggtt tcaaatacta tgcgcacttt ttgtttgtaa tcgtttccta
      841 atgccattgc accatcgagt aaaattcgcc ttgcacgatc tatttcattc gataacactt
      901 cgtgttgaac aaaatggtag tcagataaat cttctttagc aatgatggtg agttccatat
      961 tgattgaatt tggtgtatca gagttttagc ggcatataaa agtaggcatt tattctaata
     1021 tgttcaataa aattttaaaa cggccatttt tcccgacttt ccggccatcc agaccttttt
     1081 ttgtgaaggt gctatgctga tggtgtaaaa tgctagtgat agtccatctg cgtttttaat
     1141 ttctttaatg tagtgctgac tgtcaatcac atacgtgccg ttatggcctg ttgtaatcag
     1201 gtagttgcca tttttagttt ttattgtttt aacacatgac acaaaagccc cctcaggaag
     1261 ttttatgggt tgccatgtct tgccaccatt gtttgtttgg tacaaagttg tggtagtatc
     1321 ggattgcaaa tagttgcctc caccaatata gccatttagt ttgttttcaa aatacatagt
     1381 aaaaattcct gtcatggtgc tgtcgctaat cataggtgtg gtttgtggaa gaaatgcacc
     1441 atcattttca gaaataaaaa gtcttgatgt gcttcctcct gttgcaaaaa tcacgctgtt
     1501 ttgcagccat tgtatgttgg tgttacttgc agcaaagcaa ccttcacctt ttattgcagg
     1561 tgatgtaact tttagctcct gccagttgtt gccgccatct gttgttttaa taatcataaa
     1621 atacccttcc attggatcgc ttatagcaat gccatgaagc gaatctttaa atgtcatact
     1681 atcaaaaaaa atacccttgc tgtgattgct gtaaacatgt tgccatgatg taccataatt
     1741 ctttgttctc aatagatatg ccggtgaatc aatgctcaat aacaaaaccg ttgagtcgtt
     1801 taatgccgcc aatgacctga attcagaata ttttccatca gtggcaatag agtctatatg
     1861 ccagttgctg ccaccatctt ttgtaaaacc aaaaacacct ctgttagctg caaaccacac
     1921 cacactgtcg tttactgtct gtagtgcgcg aatgcttacc tgaacgggca attgaatggt
     1981 ttttaattct atctgctgtg cctgcagtga tgatgtaaaa caaaagcac
//
LOCUS       DAORQT010000289         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_11764_length_2023_cov_15.162093, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000289 DAORQT010000000
VERSION     DAORQT010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11764_length_2023_cov_15.162093"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="PK134_08180"
     CDS             <1..1997
                     /locus_tag="PK134_08180"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRB38866.1"
                     /translation="NKSSMKDGIIHFGGGCTGEIVSPDGLILTNHHCGLDYIQSLSSL
                     DHDYLTNGFWATARDKELPAPGLSVKFLIRMEDVTQKVKAEISDTLSEADLQAKLPGI
                     YSKLTKEATAGNEFYTADVRAMYGGNEYFLFVYERYEDVRLVGTPPMSIGNFGGDTDN
                     WMWPRQTGDFSMFRIYTTPDGKPAKYAKENVPLKSKYYFPVSIKGVKEGDFAMIMGYP
                     GRTDRYATSYSIENGLDITSPSLVKLRTKRLDVINNAMADNDAIRIKYQAKRNQVSNY
                     WKYFIGQQKQLKRMKVVEQKRNLENQFTTWVNADKKRRLHYGFVMGNLQQSYDNARKY
                     AKLRVYTSEALMGTEILRYAFSFSALDKALNEKEPNADDIKAKAAMLKSRVEDYFEEY
                     DAGVDQKVFAVLVKSFADDVAKDQQSPIFAEMAKKYKGDYNKMAAAVFAKSIFSDKNK
                     VLAFLENPLAKKLKNDPAFKLINTQLSFYDDNFRAPIRTIDLALAANNRLYIEGLREM
                     MPDKKLYPDANSTMRLSYGSVRAYDPADAVKYSYFTTIEGVMQKMDNSNTEFKVPEKL
                     VELYNKKDYGRYGSNGTLNTCFLTNNDITGGNSGSPVINGNGEIIGLAFDGNWEAMSG
                     DIKYDEEYKRTICVDIRYVLFCIDKFGGAKHIVDEMKVVE"
BASE COUNT          678 a          386 c          413 g          546 t
ORIGIN      
        1 tcaacaaatc atccatgaaa gatggtatca ttcactttgg tggtggatgt acaggcgaaa
       61 ttgtttcgcc cgatggatta attcttacca accatcactg cggacttgat tacattcaat
      121 cgttgagtag tttagatcat gattatttga ctaacggttt ttgggcaaca gctcgcgata
      181 aagaacttcc tgcaccaggt ctttcagtaa aattccttat ccgcatggaa gatgttacac
      241 aaaaagtaaa ggcagaaatc agcgacacac tttccgaagc tgatttacaa gccaaactac
      301 ctggaatcta ttccaagctg actaaagaag caactgcagg taatgagttt tatactgccg
      361 atgtgcgtgc aatgtatggt ggcaatgaat attttctttt tgtttacgaa agatacgaag
      421 atgtgcgttt ggttggaaca cccccaatga gcattggtaa ctttggtggc gacaccgaca
      481 actggatgtg gccacgtcag acaggcgatt tttctatgtt tcgtatctac acaactcccg
      541 atggaaaacc tgcaaagtat gctaaagaaa atgttccgtt aaaatccaaa tactattttc
      601 ctgtcagcat taaaggtgtt aaagaaggcg attttgctat gattatgggc taccccggtc
      661 gcaccgatcg ttatgcaact tcatacagca ttgaaaatgg tcttgacatt acaagtccat
      721 cgttagtgaa acttcgtacc aaaagattag atgtcatcaa caatgcaatg gcagataatg
      781 atgctataag aataaaatat caggctaagc gcaatcaggt aagcaactac tggaaatatt
      841 ttatcggtca gcagaagcaa ttaaaaagaa tgaaagttgt tgaacaaaaa cgcaacctcg
      901 aaaatcaatt cactacatgg gttaatgccg ataaaaaaag aagactgcac tatggatttg
      961 tgatgggcaa ccttcagcaa tcttatgaca atgccagaaa atatgccaag ctgcgtgttt
     1021 atacttcaga ggcattaatg ggaacagaaa ttttgcgtta tgcattttcg ttttctgctt
     1081 tagacaaagc gcttaacgag aaggaaccaa atgccgatga tataaaagca aaagcagcaa
     1141 tgcttaaatc aagagtagaa gattattttg aagagtacga tgccggtgtt gaccaaaaag
     1201 tttttgccgt tcttgtaaaa tcgtttgccg atgacgttgc aaaagatcag cagtcaccca
     1261 tctttgcaga aatggctaag aaatataaag gcgactacaa caaaatggct gctgcagttt
     1321 ttgcaaaaag tattttctcc gacaaaaaca aagtgcttgc attcttagaa aatcctttag
     1381 caaagaaact taaaaacgat ccagcattta aactcataaa cacacagttg agtttttatg
     1441 atgacaactt ccgtgctcct atccgcacca ttgatttagc cttagctgcc aacaacagat
     1501 tgtatattga aggccttcgc gaaatgatgc ctgataaaaa actctatccc gatgccaaca
     1561 gcaccatgcg tttaagctat ggctctgtgc gtgcttacga tcctgccgat gctgttaagt
     1621 acagctactt tacaaccatt gaaggtgtga tgcagaaaat ggataacagc aatacagaat
     1681 ttaaagttcc agaaaagtta gttgagctgt ataacaaaaa agactacggt cgctatggca
     1741 gcaacggaac attaaacacc tgcttcctca caaacaacga cattacaggt ggcaacagcg
     1801 gaagtcctgt tatcaatggt aatggcgaaa ttatcggact tgcattcgat ggcaactggg
     1861 aagctatgag tggcgatatt aaatatgacg aagaatacaa acgcactatt tgtgttgaca
     1921 tcagatatgt tttgttttgc attgacaaat ttggtggagc caaacacatt gttgacgaaa
     1981 tgaaagttgt ggagtaacta catcacaaaa taaaatagca gag
//
LOCUS       DAORQT010000290         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12053_length_1991_cov_6.342459, whole genome shotgun sequence.
ACCESSION   DAORQT010000290 DAORQT010000000
VERSION     DAORQT010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12053_length_1991_cov_6.342459"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK134_08185"
     CDS             <1..416
                     /locus_tag="PK134_08185"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRB38867.1"
                     /translation="QKFEEVVCATIEEHMLTPEIEIDAELKLESITKPFYNVLKQFAP
                     FGPGNMSPVFVSRNCIADKNQTRPVGQENKHLKLAFTQDGFGKNFSAIGFQLGEYADL
                     LKSGKRFDVCYHIEENHFNGNTELQLNIKDIKISE"
     gene            complement(408..716)
                     /locus_tag="PK134_08190"
     CDS             complement(408..716)
                     /locus_tag="PK134_08190"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRB38868.1"
                     /translation="MAKKFSVKTNLHRRDIRGVSTYVVLREASDHFNETGVIEVIGFI
                     DNHPIEGALQPKTDGSHWLTIKKEWREAIGKTIGDSVEISIEIRKKISPPQKAKKKLF
                     "
     gene            complement(822..>1991)
                     /locus_tag="PK134_08195"
     CDS             complement(822..>1991)
                     /locus_tag="PK134_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRB38869.1"
                     /translation="DITRAASLAKQAAEKGIKAKSEFTITPGSEKVRYTVERDGYLGI
                     FEKIGGVVLANACGPCIGQWARHTNDPNKKNSIITSFNRNFAKRNDGNPNTHAFVASP
                     EIVTALAIAGDLTFNPVTDFITNDKGEKVKLAEPQGIELPIKGFAVEDAGFQAPAKDG
                     SKVEVIVSPTSDRLQLLAPFAAWEKSDLKGLKLLIKAKGKCTTDHISMAGPWLKYRGH
                     LDNISNNMLIGAVNAFNDNTNSVKNQLDGSYDEVPKVQRAYKAKGIGSIVVGDENYGE
                     GSSREHAAMEPRFLGVRAVLVKSFARIHETNLKKQGMLALTFADKADYDKIKEDDTFD
                     IIGLTSFTPGVPLTLVLHHADGSKEEIKVNHSYNEHQIAWFKAGGALNIIRSQQA"
BASE COUNT          557 a          402 c          374 g          658 t
ORIGIN      
        1 aacaaaagtt tgaagaagta gtttgtgcta ccattgaaga gcacatgctt acaccggaga
       61 ttgaaattga tgcagagcta aagttagaaa gtataacaaa accattctat aatgtgctga
      121 aacaatttgc accatttggt cccggtaata tgtcgcctgt gtttgtcagc cgcaattgta
      181 ttgccgacaa aaatcagaca cgacctgttg gtcaggaaaa caaacatctg aaacttgctt
      241 tcacacaaga tggatttgga aagaattttt ctgccatagg gtttcagtta ggcgaatatg
      301 ccgatttact caaatcagga aaacgctttg atgtttgtta ccatattgaa gagaatcatt
      361 ttaatggtaa cacagagttg caactcaaca ttaaggatat aaaaatttca gaatagtttt
      421 ttctttgctt tttgtggagg tgatattttc tttcttatct caatagaaat ttcaacacta
      481 tcaccaatgg ttttgccaat agcttcgcgc cattcttttt ttattgtcag ccaatgtgag
      541 ccatctgttt ttggttgcaa ggcaccttca ataggatgat tgtctataaa tcctattact
      601 tcaataacac ccgtttcgtt aaagtggtca cttgcttctc tcaacaccac ataggtagaa
      661 acacctctga tgtctctgcg atggagattt gtttttacac taaacttttt cgccatagca
      721 atgctgtttt tattttaccc tataaagata agaaaataaa aatgaaaaac tccggaataa
      781 attttttatt ccggagtttt ctatttgact gaattttaaa atcaggcttg ttggctgcga
      841 atgatgttta gtgcaccacc tgctttgaac caggcaatct gatgttcgtt atacgaatga
      901 tttactttaa tttcttcttt tgagccatca gcatgatgca ataccaatgt taatggaaca
      961 ccgggtgtga atgatgtcaa accgataata tcaaaagtat catcttcctt aattttatca
     1021 taatctgctt tatcggcaaa cgtcagtgca agcattcctt gtttcttaag attggtttcg
     1081 tgaatgcggg caaacgattt taccaacact gcacgtacgc ctaagaaacg cggttccata
     1141 gctgcatgtt cacgcgatga gccttctcca tagttttcat cacccacaac aatagagcct
     1201 attccttttg ccttataggc acgttgcact ttaggcactt catcatagct gccgtcaagt
     1261 tgattcttta cactgttggt gttatcatta aatgcattta ctgcaccaat aagcatgttg
     1321 ttggaaatgt tatcgagatg tcctctgtat ttcaaccacg gacccgccat tgaaatatga
     1381 tcggtagtac atttgccttt tgctttgata agaagtttca agcctttaag gtctgacttt
     1441 tcccaagcag caaatggtgc tagcaactgt aagcggtcgc ttgttggcga cacaatcact
     1501 tcaactttac tgccatcttt tgccggagcc tgaaagcctg catcttcaac agcaaaacct
     1561 ttaataggaa gttctattcc ttgcggttcg gctaacttaa ctttttctcc tttatcgttt
     1621 gtgataaaat ctgtaaccgg attaaaagta aggtcgcctg caatggctaa tgcagttact
     1681 atctctggcg aagcaacaaa ggcatgtgta tttgggttgc catcatttct ctttgcaaag
     1741 ttgcggttga aagatgtgat gatagaattc tttttgtttg gatcgttggt atggcgagcc
     1801 cactgaccaa tgcaaggacc acaggcattt gctaaaacca cacctcctat tttttcgaaa
     1861 ataccgaggt agccatcgcg ttcaacagta taacgaacct tctcactgcc gggtgtaatt
     1921 gtaaattcac ttttagcttt gattcctttt tctgctgctt gttttgctaa tgaagctgca
     1981 cgtgtaatgt c
//
LOCUS       DAORQT010000291         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12134_length_1981_cov_3.762721, whole genome shotgun sequence.
ACCESSION   DAORQT010000291 DAORQT010000000
VERSION     DAORQT010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12134_length_1981_cov_3.762721"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1294)
                     /locus_tag="PK134_08200"
     CDS             complement(32..1294)
                     /locus_tag="PK134_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRB38870.1"
                     /translation="MQNNTEPLKNNITIVGAGPAGATASMFLSSFGIKHTLIDKAVFP
                     RDKICGDALSGKVLPVLKKLNPDLIQQLLVQNEKCLGSNGILFGAPNGKTLEVPFKAV
                     NSTLNHSPGYVSKRIDFDFSLIKLLNPEYCNFLQGHEVKKITKGEEGVTLNVLNTQNK
                     ETYNVSSSLVIACDGAHSICNKLFPDHKLDAGHYCGGVRAYYSGVTGFHSENFIELHF
                     LKEMLPGYFWIFPLPNGMANVGAGMLTRTISSKKVNLKEAMLLAIKNNPSIKDRFKDA
                     NIIGNIDGWGLPLGSKKRKLSGNRIILCGDAASMIDPFSGEGISNAMYCGMRAAEVSR
                     DAINSSNYTDDFLSGYDQFVYNRLWNELKLSHTLQKLCNYPSLFNFVVNKASKNETFR
                     QTISCMFDDIDLREKLRKPSFYFKMLFG"
     gene            complement(1305..>1981)
                     /locus_tag="PK134_08205"
     CDS             complement(1305..>1981)
                     /locus_tag="PK134_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HRB38871.1"
                     /translation="LDYMFRKAKMTNAQGLQYLKDHGLDSIPGGGAEIFDEEIRNKIC
                     ADKCNAEEWLNIHQEAHQLNIPSNATMLYGHYESYSHRVDHMNRLRNLQDKTKGFQTF
                     IPLKFRNKDNEMSHLSETTQVEDLKNYAVARIFLDNFQHVKAYWPMIGRATAQLSQNF
                     GVDDMDGTIDDTTRIYTMAGSEEQNPTLTTQELVDLISQNGFVAVERDTLYNHLKVYE
                     NVPVAN"
BASE COUNT          594 a          390 c          329 g          668 t
ORIGIN      
        1 agtatttgca atagaaacta aattgttgct atcagccaaa aagcatttta aaataaaagg
       61 atggcttgcg tagtttctct cttaaatcta tatcatcaaa catacagctt atggtctgac
      121 ggaaggtttc gttttttgac gccttattga ctacaaaatt aaacagcgaa ggataattgc
      181 aaagtttttg aagtgtatga cttaatttta attcattcca aagtctgtta taaacaaact
      241 gatcataacc tgataaaaaa tcatcggtat agttgctgct atttattgca tcacgcgaca
      301 cttctgcggc acgcatgccg cagtacatgg cattgctgat accctcacca ctgaatggat
      361 caatcatgct tgctgcatca ccacataaaa taattctgtt gcctgataat tttcttttct
      421 tagaacctaa tggcaatccc catccatcaa tgttgcctat aatgtttgca tctttaaaac
      481 gatcttttat ggaaggatta tttttaatgg caagaagcat agcttctttc agatttacct
      541 tttttgaact gatggttcgt gtcagcatac ctgcaccaac attagccatg ccattgggta
      601 atggaaatat ccagaagtat ccgggtagca tttctttcag aaaatgtaac tcaataaaat
      661 tctcactatg aaaccctgtc acaccggaat agtatgcccg aacgcctccg caatagtgtc
      721 ctgcatcgag tttgtgatca ggaaataatt tattgcaaat gctatgtgca ccatcacagg
      781 caatgactaa agatgaactt acattgtaag tttctttatt ctgtgtattt aaaacattca
      841 gtgtgacacc ttcttcgcct tttgttattt tctttacttc atggccttgc aggaaattac
      901 aatactccgg gttcaataat tttattaatg aaaaatcgaa atcaattcgt ttactcacat
      961 atccgggtga atgatttaaa gttgagttta cggctttaaa aggtacttct aatgttttgc
     1021 cattgggtgc accaaataaa attccattgc ttcccagaca tttttcgttt tgtactaata
     1081 gctgctgaat taaatcggga ttaagtttct tcaatacagg tagaactttt ccgcttaatg
     1141 catcaccaca aattttgtcg cgcggaaaaa cagctttatc aatcaaagta tgtttgattc
     1201 caaaggaaga tagaaacata gatgccgttg cacctgctgg gcctgcacct actatagtta
     1261 tgttgttttt taatggctca gtattattct gcacaataaa tctttcaatt agctactgga
     1321 acattttcat aaactttcaa atgattgtac agtgtatcgc gttcaacggc aacaaaacca
     1381 ttctgcgaaa tcaaatcaac taattcttgc gtggttaaag ttggattttg ttcctcagaa
     1441 cctgccattg tataaatgcg tgttgtgtca tcaatagtgc catccatatc gtcaacacca
     1501 aaattttgtg agagttgtgc agtggctctg cctatcatgg gccaataagc ttttacatgc
     1561 tgaaagttat ctaaaaaaat ccgggcaaca gcataatttt ttaaatcttc tacctgagta
     1621 gtttcagaaa gatggctcat ctcattatcc ttattcctga attttaaggg aataaatgtc
     1681 tgaaagccct tagtcttatc ctgcagattt cttaatctgt tcatgtggtc aacacgatga
     1741 gaatagcttt cataatgacc atacaacatt gttgcatttg agggtatgtt taattgatgt
     1801 gcttcctgat gaatattcaa ccactcctca gcattacact tatctgcaca gattttattg
     1861 cgaatttcct catcaaaaat ttctgcacca ccaccgggaa tactatctaa tccatgatct
     1921 ttcaaatatt gaagtccttg tgcatttgtc atttttgctt tgcgaaacat atagtccaat
     1981 t
//
LOCUS       DAORQT010000292         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12188_length_1974_cov_6.294424, whole genome shotgun sequence.
ACCESSION   DAORQT010000292 DAORQT010000000
VERSION     DAORQT010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12188_length_1974_cov_6.294424"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /gene="nuoF"
                     /locus_tag="PK134_08210"
     CDS             complement(<1..1227)
                     /gene="nuoF"
                     /locus_tag="PK134_08210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRB38872.1"
                     /translation="MSRKLLLDNLDKPGIQSITGYSDNGGYEALRKALQMKPEEIVSE
                     VQKSGLRGRGGAGFPPGMKWSFIAKPEGVPRYLVCNADESEPGTFKDRYLMEHKPHIL
                     IEGMIISSFALGANTSYIYIRGEMMYVFHILQKAIDEAYAAGYLGKNILGSGFNLDLH
                     VHCGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGLWGCPTVVNNVETIAAVVPI
                     IKITGEEYAKIGIGKSTGTKLISASGHINKPGVYEIELGVPVEEFIYSDAYCGGIKNG
                     KQLKAVVAGGSSVPVLPAHLILKTAAGEPRLMTYESLADGGFATGTMLGSGGFIVMDE
                     DTSVVKNLWTFAHFYHHESCGQCSPCREGTGWMYRILNKILNGKGEMKDIDLLWNIQG
                     NIEGKTNCPLGDAAAWP"
     gene            complement(1238..1780)
                     /gene="nuoE"
                     /locus_tag="PK134_08215"
     CDS             complement(1238..1780)
                     /gene="nuoE"
                     /locus_tag="PK134_08215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRB38873.1"
                     /translation="MSTNKNIQFSDDAMALVQKIINRYPEGKQKSALLPILHIAQAEF
                     DGWLSPEVMDYVASLLKIQSIEVYEVATFYSMFNMKPVGKCLIEVCRTGPCWLMGAED
                     VVRYIEKKLGIKAGETTADGMFTLKTVECLASCGTAPMMQIGETYHENLTLEKVDDIL
                     NDHKNQGNRRTHWEKDKVKP"
     gene            complement(1795..>1974)
                     /locus_tag="PK134_08220"
     CDS             complement(1795..>1974)
                     /locus_tag="PK134_08220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087729.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRB38874.1"
                     /translation="GELGYFLISDGGRTPYRLHIRRPCFIYYQAYPEMIKGAYLSDAI
                     LTMSSMNVIAGELDA"
BASE COUNT          528 a          449 c          331 g          666 t
ORIGIN      
        1 aggccatgct gctgcatcgc ctaaaggaca atttgttttt ccttcaatat ttccctgaat
       61 gttccacaac aaatcaatat ctttcatttc acctttgccg tttagaattt tatttaatat
      121 tctgtacatc catcctgttc cttcacggca tggcgaacat tgtccgcaac tttcgtgatg
      181 ataaaaatgc gcaaacgtcc acaggttttt tactacgctg gtatcttcgt ccatcacaat
      241 aaaaccacct gaaccaagca tggtgcctgt agcaaatcca ccatcggcaa gactttcata
      301 agtcatcaga cgaggttcgc ctgctgcagt ttttaaaatg aggtgtgcag gtagaacagg
      361 aacagaagag cctccggcaa caacagcctt taactgtttt ccgtttttta ttcctccgca
      421 gtatgcatcg ctataaataa attcttcaac aggaacaccc agttcaattt cataaactcc
      481 gggtttatta atgtgtcctg atgcggaaat taattttgtg cctgtacttt ttccgatacc
      541 gatttttgca tattcttcac cggttatttt tattatcggt acaactgcgg caatggtctc
      601 tacgttattt accactgttg gacatcccca caaacctttc actgccggaa aaggaggttt
      661 aattcttggg tttcctcttt taccttctaa tgattctatc aatgctgttt cttcaccaca
      721 gatatatgca cctgcaccac agtgtacatg caggtcgaga ttaaagccgc ttcctaaaat
      781 attttttccg agatatcctg ctgcataagc ttcgtcaatt gctttttgaa gtatgtgaaa
      841 tacatacatc atttcacctc tgatgtagat gtaggaagta tttgccccta aggcaaaact
      901 tgaaattatc attccctcaa ttaaaatatg aggtttgtgt tccatcagat accggtcttt
      961 aaaagttccg ggttcacttt catcggcatt acacaccaaa taacgtggca caccttcggg
     1021 cttagcaatg aaactccact tcataccggg tggaaaaccc gcaccgccac gtccacgcaa
     1081 tcctgatttt tgtacttcac ttacaatttc ttcaggcttc atttgaagtg ctttgcgcaa
     1141 agcttcgtat ccgccattgt cgctgtagcc ggttattgac tgaatgcccg gcttatctaa
     1201 gttatctaaa agtaatttac gtgacatact ttttctttca gggttttact ttatcttttt
     1261 cccagtgcgt tcttctgttg ccctggtttt tatgatcatt taaaatatca tcaacttttt
     1321 ctaaggtcag attttcgtga taggtttctc cgatttgcat cattggtgct gtgccacaac
     1381 ttgccaaaca ttcaactgtc tttaaggtaa acataccatc ggcagttgtt tctccggctt
     1441 tgattcctaa tttcttttca atataacgaa caacatcttc tgcacccatc agccaacaag
     1501 gtcctgtgcg gcaaacttct atcagacact tacccacagg tttcatgttg aacatggagt
     1561 agaatgtagc tacttcataa acttcaatag actgtatttt tagtaatgat gctacataat
     1621 ccatcacttc aggacttaac caaccgtcaa attctgcttg tgcaatatgt agtattggaa
     1681 gcagtgcaga tttttgtttt ccttcaggat atctgtttat tattttttga accaatgcca
     1741 tagcatcgtc cgaaaattgt atgtttttgt ttgtgctcat gttacatcat taatttatgc
     1801 atccagttca ccggctatta cattcattga actcattgtt aatatagcat cactcaaata
     1861 ggctcctttt atcatttcag gataggcttg ataataaata aagcaaggtc gtctgatatg
     1921 taaacgataa ggagtacgac cgccatcact gattaagaaa tatcctagtt cacc
//
LOCUS       DAORQT010000293         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12226_length_1970_cov_4.608355, whole genome shotgun sequence.
ACCESSION   DAORQT010000293 DAORQT010000000
VERSION     DAORQT010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12226_length_1970_cov_4.608355"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>251
                     /locus_tag="PK134_08225"
     CDS             <1..>251
                     /locus_tag="PK134_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38875.1"
                     /translation="KSKTINVNAASSVSASIYTNDPTTLCNQGSVTMHVNIPIPGYSY
                     QWKNNGNNISGATATSYTTNVSGNYSCNVSASCGNTNSNA"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <262..1248
                     /locus_tag="PK134_08230"
     CDS             <262..1248
                     /locus_tag="PK134_08230"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38876.1"
                     /translation="IGSFNAQISPSGSQILCTGGSMLLSASSGIGFTHQWYKNGNAIS
                     GATSSTYSATSGGSYTVFISSPCGSGTSPAAVLTENTVSASVSPAGTSTVCAGIPFTF
                     TANSGAGYSYRWYRNNVLLAGTGQSINATSAGQYKVEVSINGSCAVISNIAVLDVINN
                     PAPVISPANATICNGAAQVFTANNFTGVTYQWYRNSVLIPGATAQTYSATLAGQYKVT
                     QTANGCYKSKSAQLTVTSCREEGEGFIAEAQSNISISPNPFTDQLNIYVPSEKDGNAE
                     IQVLDMLGKVVYRSVITLNQTSEINLHVPAGIYMLSVHTDGKYQVQKIVKSR"
     gene            1361..1798
                     /locus_tag="PK134_08235"
     CDS             1361..1798
                     /locus_tag="PK134_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB38877.1"
                     /translation="MSGVNKVILVGNLGKDPEMRHIDGNISVAKFPLATSEVYKNKDG
                     QRVTQTEWHNVVLWRQLAETAEKFLKKGSLVYIEGKLRTRNWDDKDGNKKYITEVIGD
                     SMTLLGRKPDENGNPSTGTTERTTSEHPASEAGHSEINDDLPF"
BASE COUNT          639 a          383 c          389 g          549 t
ORIGIN      
        1 aaatctaaaa ctattaatgt aaatgctgca tcttctgttt ctgcatctat ctacaccaac
       61 gacccaacaa cactctgtaa tcagggttcg gtaacaatgc atgtaaacat tcctattccg
      121 ggatatagct atcagtggaa aaataatggt aacaacattt caggcgctac tgcaacaagt
      181 tatactacca atgtttcagg aaactattca tgcaatgtta gtgcaagctg tggcaacaca
      241 aacagcaatg cnnnnnnnnn natcggaagt ttcaatgcgc agataagtcc atcaggcagt
      301 cagattcttt gtaccggtgg ttctatgttg ttgagtgcat catcaggaat tggttttaca
      361 catcagtggt ataaaaacgg taatgcaatt tctggagcaa caagctcaac atatagtgca
      421 acttcaggag gaagttatac agtgtttatt tcatcgcctt gcggcagtgg aacatcacct
      481 gctgctgtgt taactgagaa tactgtctct gcctctgtaa gtccggcagg tacatcaaca
      541 gtttgtgcag gtattccttt tacatttact gctaatagcg gtgccggtta ttcctatcgc
      601 tggtatagaa acaatgtgtt gctggcagga acgggacaaa gtattaatgc tacttctgca
      661 ggacaatata aagttgaagt ttcaatcaac ggttcatgtg ctgtaatcag taacattgct
      721 gttttagatg tcatcaacaa tcctgctccg gtaatttcgc ctgcaaatgc aactatttgt
      781 aacggagctg cacaggtatt cacagcaaat aattttacag gggtgactta ccaatggtac
      841 agaaacagtg tattaattcc gggggcaaca gcacaaacct attctgcaac attagcagga
      901 caatacaagg ttacacaaac tgcaaatgga tgttacaaat caaaatctgc tcaactcaca
      961 gtaacttctt gtcgcgaaga aggtgaaggc tttattgctg aagctcaaag caacatcagc
     1021 atatcgccaa atccatttac agaccaactc aatatttatg ttccatcaga aaaagatggc
     1081 aatgctgaaa ttcaggtttt ggatatgtta ggtaaggttg tatatcgttc tgtaattacc
     1141 ttaaatcaaa catcagaaat taacctgcat gttccggcag gaatttacat gctgtctgtt
     1201 cacactgatg gaaaatatca ggtgcagaaa attgtgaaat cgagatagta aagtcgttca
     1261 taaaaaaaga ccccgctgct gcggggtttt tttatgacca aattgttata tttgtatgca
     1321 caaacaaaca cattcgttac gcagttaaac aacagcaatc atgtcaggag taaataaagt
     1381 aattttagta ggcaacttag gaaaagatcc ggaaatgcgt catatagatg gtaacatcag
     1441 tgtagctaaa tttccattgg caacatcaga agtatataag aataaagacg ggcaaagagt
     1501 aacacaaaca gaatggcata atgtggtttt gtggcgacaa cttgcagaga ctgcagaaaa
     1561 attcctgaag aaaggtagct tggtatatat tgaaggaaaa ctacgcacac gcaactggga
     1621 tgataaagac ggcaataaaa aatacataac agaagtcatt ggcgacagca tgacacttct
     1681 tggtcgcaag cccgatgaaa acggaaatcc ttcaacaggc actactgaga gaacaacatc
     1741 tgaacatcct gcatctgagg ccggtcattc agaaataaat gatgatttac cattttaagc
     1801 gcgttgcata gtttttaata cccaactaag caacatcatc ctatatttgc aggaaattat
     1861 attgtactca ccccctcagc atgcctgtcg tcagtagctt gttacttagc aatttttcat
     1921 cctattcatt taccggtgag ctattagtta ttcttgtgtt gcttctactt
//
LOCUS       DAORQT010000294         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12302_length_1963_cov_4.844340, whole genome shotgun sequence.
ACCESSION   DAORQT010000294 DAORQT010000000
VERSION     DAORQT010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12302_length_1963_cov_4.844340"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK134_08240"
     CDS             complement(<1..286)
                     /locus_tag="PK134_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38878.1"
                     /translation="MSPSKNNYSTAQAFRQTYKSLQDHLSPLQKKRIKWIAFLIFLSA
                     ILDVFGLASVLPLVKMATEPEIIHTNQYLSTAYSYLNFETDKSFMVFCIVI"
     gene            complement(301..>1963)
                     /locus_tag="PK134_08245"
     CDS             complement(301..>1963)
                     /locus_tag="PK134_08245"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRB38879.1"
                     /translation="YKSALSLEKDEDASVININIADEVEEKAVDFLDTLTNLYINYSI
                     SVARGINENSLKFIEAQLKDYEDQLNGVETSLVEYQQQSGAIDVANQQSTFLKEKIDV
                     QGEKAKLTARLQSVEYVYNQLTSGSSDFSTLSPALMTEQSNPGLTSAFNELSSLMQRK
                     VNMSFSMTPNSPQIKDVDVLINKAKENVIGIIMNIRKDIAMQINLLSSREGSFAASLN
                     RMPSTIKGLSDITRKKEINEKMYLFLLESRAQNVIASAAIVPDKSILEPASSTGLLRP
                     IRNKMLFMGAGVGLALAFALVFLKSIFLNYIFTKEELADITNQPIIGVIGKSAEAKKD
                     YLVVHEHPQSLAAEAFRVIRTNLSYFAPKTSSKVILFTSTISGEGKTFCAVNTATMLA
                     RAKKKVVVVDLDLHKPKQAVAWNMTNDVGVTSYIVGKATLKDTLKDTPVENLKVILSG
                     PKSPNASELILDSMMEQLINELKEHFDYIILDMPPVGLLSDALVMMKHSDLNLYVMKA
                     GYSKKDFVEVAHQLMEKNQIKSLSFILNGVNPKNMPVGYGGAYYK"
BASE COUNT          582 a          356 c          328 g          697 t
ORIGIN      
        1 taataacaat acaaaatacc atgaaagatt tatctgtttc aaaattcaga taactatatg
       61 ctgtacttaa atattggttg gtgtgaataa tttcaggttc agtagccatt tttactaatg
      121 gaagaacaga agctaaacca aacacatcta agattgcaga tagaaaaatc agaaatgcaa
      181 tccatttaat ccgttttttt tgcagtggag ataaatggtc ttgaagactt ttataagttt
      241 gcctgaaggc ttgagctgta gagtagttgt ttttgctggg cgacatactt tttgttcgtg
      301 ttatttataa tatgcaccac catacccaac aggcatgttt ttcgggttta caccatttaa
      361 gataaagctc aggctcttga tttgattctt ctccattaac tgatgagcaa cttcaacaaa
      421 gtctttcttg ctgtatccgg ctttcatgac atatagattt aggtcggaat gtttcatcat
      481 cactaaagca tctgacaaca atcctaccgg tggcatatct aaaatgatgt aatcgaaatg
      541 ttctttcaat tcattaatta attgttccat catcgaatct aaaatcaact cagaagcatt
      601 tggtgatttt ggtccgctta aaataacttt tagattttca acaggagtgt cctttaaagt
      661 atcttttaag gtagctttac caacaatata agatgttaca cctacatcgt tagtcatgtt
      721 ccatgcaaca gcttgtttgg gtttgtgtaa atccaaatca actaccacaa ctttcttttt
      781 agctcttgct aacatggtcg ctgtattcac tgcacaaaaa gtttttcctt ctccggaaat
      841 agtagaagtg aataaaataa ctttcgaact tgttttaggt gcgaaatagg ataagttggt
      901 gcgaattact ctgaaagctt ctgctgcaag tgattgtgga tgttcatgca caacaagata
      961 atctttcttg gcttctgcac tttttcctat aacaccgata atcggttgat tggtaatatc
     1021 ggcaagttcc tctttagtaa aaatataatt aaggaaaata ctttttagaa agactaaagc
     1081 aaatgcaagt gccagaccca ctcctgctcc cataaataac attttgtttc gaatgggacg
     1141 tagcaatcct gtacttgatg cgggctcaag aatagatttg tcaggtacaa tggctgccga
     1201 tgcaattaca ttctgtgccc tgctttctaa taagaacaaa tacatctttt cattaatctc
     1261 tttttttctt gtaatgtcac taaggccttt aatggttgag ggcattctgt ttaatgatgc
     1321 tgcaaaacta ccttcacgac ttgacaataa gttaatttgc atggctatat ccttacggat
     1381 gttcatgata ataccaataa cattttcttt tgctttatta attaaaacat ctacatcctt
     1441 tatctgcgga ctgttgggtg tcatgctaaa tgacatgttt acctttcgtt gcatcaagga
     1501 acttaattcg ttaaatgctg aagtcagtcc gggattagat tgttctgtca ttaaagccgg
     1561 tgaaagcgta ctgaagtcac tactgccact tgttaattga ttataaacat attctacaga
     1621 ttgtaatcgt gctgtaagtt tagctttctc gccttgcaca tcaatttttt cctttaaaaa
     1681 agtactttgt tgattggcta catcaattgc gccactttgc tgttggtatt caaccaagct
     1741 tgtttcaaca ccattaagtt gatcttcgta gtcttttaat tgtgcttcaa taaattttaa
     1801 tgaattttca ttaattcctc ttgctactga aattgaataa ttgatataaa gatttgttaa
     1861 cgtatccaga aagtcaacag ctttttcttc tacctcgtca gcaatattga tatttattac
     1921 agatgcatct tcgtctttct ctaaactgag ggcacttttg tat
//
LOCUS       DAORQT010000295         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12310_length_1962_cov_7.039329, whole genome shotgun sequence.
ACCESSION   DAORQT010000295 DAORQT010000000
VERSION     DAORQT010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12310_length_1962_cov_7.039329"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1457)
                     /locus_tag="PK134_08250"
     CDS             complement(174..1457)
                     /locus_tag="PK134_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRB38880.1"
                     /translation="MISDFLPAILASLAFVAFYSGIEIAYLSSNKFKIELDSKQGKWS
                     GKILSYFSKSPSRFICTILVGLNISLVIYGTNMSGLLNPYLQQWLPQQLNSEIGRLII
                     ETLITTFFLLLAGEFLPKILFSINPNDTLNFFSGFIWISYILLFPLVWLILKLAHFLL
                     RNVFKVDFVEETSGFGRVDLDNFIEEISENTGGKSEHSKEIQMFQNALDFDSLKVRQC
                     MIPRNEIISLSSKESVETLRKKFVETGLSKIMIYQDTKDNIIGFVHSYEMFKNPESIM
                     SVLLPVSIFPETLPARELLTHLTANHRSVAIVVDEYGTTSGMVTIEDIIEEIFGEIDD
                     EHDIDVYLEQKISETEFLFAGRLEVDYLNQKYNLELPESELYTTFAGLIFNHLEDIPH
                     VKEQFTIGSYKITVEQVSGNKIEQVRLVILVNAEN"
     gene            complement(1495..1692)
                     /locus_tag="PK134_08255"
     CDS             complement(1495..1692)
                     /locus_tag="PK134_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38881.1"
                     /translation="MKILRILEISWLVIAIGSLCAGIYKLISAGLNQAVFIFAISIIA
                     FVFFMIRRKQRIAMEKQNASR"
     gene            complement(1670..>1962)
                     /gene="lptC"
                     /locus_tag="PK134_08260"
     CDS             complement(1670..>1962)
                     /gene="lptC"
                     /locus_tag="PK134_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRB38882.1"
                     /translation="STLTSDYAVRDDFNKNMEAKKNVEVVNVKGEKLMTEYLKWDEKT
                     GLISSPEFVKIITADEIIYGNGFESNQDFSRYKIFNIKGIINLKKNENTENT"
BASE COUNT          627 a          388 c          324 g          623 t
ORIGIN      
        1 gatgtagaaa taatttttca gaaaatagaa gacctgcaca ttgcctgccc aaacaataaa
       61 ggcgattggt attttacagg aaactatcca acacccggtg gcaataaagt ggtgagcaaa
      121 gctttcatta actacatgca gggaatcaat gcccgagcct attaagagga aatttagttt
      181 tcggcattaa ctaaaatcac taatcttacc tgctcaattt tatttccgct cacctgctct
      241 actgttattt tgtaactgcc aatggtaaac tgctctttta catgcggaat gtcttcaaga
      301 tgattaaaaa ttagccctgc aaaagttgta tataattccg actcgggtag ttcaagattg
      361 tatttctgat tcagataatc aacctcaagt cgtccggcaa ataaaaattc tgtttccgaa
      421 attttctgtt ccagataaac atcaatgtca tgctcatcat caatttctcc aaaaatttct
      481 tcaataatat cttcaatagt taccatgcct gaagttgtac catattcatc aacaacaatg
      541 gcaacactac gatggtttgc agtaagatgt gtgagcaact cacgtgcggg caatgtctcc
      601 ggaaaaatag atacaggcaa cagtactgac ataatgctct cgggattctt aaacatttca
      661 tagctgtgta caaaacctat gatgttatct ttggtgtcct gataaatcat tattttagat
      721 aatcccgttt caacaaattt ctttcttaat gtctctactg actccttgct gctgagcgaa
      781 ataatttcat tgcgtggtat catacactga cgcactttca gactatcgaa atcgagcgca
      841 ttctgaaaca tctgaatttc tttactgtgc tcgctcttac ctccggtatt ttcgcttatc
      901 tcttcaataa aattatctaa gtcaaccctg ccgaagccgg aagtttcttc aacaaaatct
      961 actttaaaaa cattacgcaa cagaaaatgg gcaagtttta aaattaacca aaccaaaggg
     1021 aaaagaagaa tatagctgat ccagataaat ccactaaaaa aatttaatgt gtcgtttggg
     1081 tttatgctga ataatatttt aggtaaaaac tctcctgcca aaagcagaaa aaaagtagtt
     1141 attaatgttt cgattatcaa tcgccctatt tcgctattga gttgctgtgg tagccattgt
     1201 tgcagataag gattgagcag tccgctcata ttggtaccgt aaatcaccaa tgaaatatta
     1261 agccctacaa gtatagtgca gataaaacgt gaaggcgact tgctgaaata agataaaatt
     1321 ttgccactcc atttgccttg cttgctgtcg agctctattt taaatttatt gcttgacagg
     1381 taggcaattt ctatccccga ataaaatgca acaaaagcaa gtgatgcaag tatggcagga
     1441 agaaagtcgg aaatcattaa caggaattaa atctatttag tgctgcaaaa gtaattatcg
     1501 tgaagcattc tgcttctcca tggcaattct ttgttttcgt cttatcataa aaaagacaaa
     1561 cgctataata gagatagcaa aaataaatac agcctgattg agtcctgctg aaatcagttt
     1621 atagattccg gcacataatg aacctatggc tattaccagc cacgatatct caagtattct
     1681 cagtattttc atttttcttc aggttaataa tgcctttaat attaaatatt ttgtagcggc
     1741 tgaaatcctg gttcgactca aacccgttgc cgtaaataat ttcatcggca gtaattatct
     1801 taacaaattc cggtgaggaa ataagtccgg ttttttcatc ccactttaaa tattcagtca
     1861 ttaatttttc tcctttaaca ttcaccacct ccacattttt ttttgcttcc atgtttttat
     1921 taaaatcatc acgcacggca tagtcgcttg tcagtgtgga ag
//
LOCUS       DAORQT010000296         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12419_length_1950_cov_4.106596, whole genome shotgun sequence.
ACCESSION   DAORQT010000296 DAORQT010000000
VERSION     DAORQT010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12419_length_1950_cov_4.106596"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..>1950
                     /locus_tag="PK134_08265"
     CDS             279..>1950
                     /locus_tag="PK134_08265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HRB38883.1"
                     /translation="MEVKNTYSADSIQVLEGLEAVRKRPAMYIGDVGVKGLHHLVYEV
                     VDNSIDEALAGHCKNISVTIGEDNSITVKDDGRGIPTEMHSKEKKSALEVAMTVLHAG
                     GKFDKDSYKVSGGLHGVGVSCVNALSTHMRTEVHRNGKIYVQEYSIGKPLYSVKEIGT
                     TDYRGTIQTFKPDDTIFITTIYNYDTLASRLRELSYLNKGIRITLTDMREKDEQGNVR
                     SDEFLSELGLREFVEYIDANREKLIPECMFFESDKFGIPIEVAMQYNTSFAENLHSYV
                     NNINTHEGGTHLSGFRRALTRTLKAYAEKEGMLAKLKFDIDGDDFREGLTAVISVKVA
                     EPQFEGQTKTKLGNNEVMGYVDQAVGDMLVHYLEMHPREAKAIVNKVILAATARHAAR
                     KARELVQRKNVLTGTGLPGKLADCSESDPGRCELFLVEGDSAGGTAKQGRDRNFQAIL
                     PLRGKILNVEKAMEYKIYENEEIRNIFTALGIYRDDKQEGRPLILDKLRYHKIVIMTD
                     ADVDGSHITTLILTFFFRHMKELIEQGYIYIATPPLYLVKKGKEERYCW"
BASE COUNT          647 a          431 c          406 g          466 t
ORIGIN      
        1 gccattgatt gatggggtga taggttgcga catccttaaa aaatataaag caagcatcaa
       61 tttttctact aaaaaacttg taattaacat ctgatttttt tacattaaaa gccttaaaaa
      121 cagcgttaat aacttgctcg actttgttaa aaaaaacgtg catttccaca ccgtttttat
      181 gtgaaagcag cctttatttt ttgtaaattt gaacaattat aaaaaacgac agtttacgaa
      241 aagttatgag tgatcaaaca gtagaaaatt tagctgaaat ggaagtaaaa aatacttatt
      301 cggccgacag cattcaggta ttggaggggt tggaggcagt aaggaaacgc cctgccatgt
      361 acattggcga tgttggtgta aaaggattgc accacttggt ttatgaggta gttgacaatt
      421 caattgacga agcccttgcc ggacattgca aaaacatcag tgtcaccatt ggagaagaca
      481 actcaataac tgttaaagat gatggtagag gtattcctac cgaaatgcac agcaaagaaa
      541 aaaagtctgc actcgaagta gccatgacag tgctgcatgc cggtggcaaa ttcgacaagg
      601 actcttataa agtttccggt ggattacatg gtgttggtgt atcgtgcgta aatgcattga
      661 gcacacacat gcgcacagaa gtacaccgca acggaaaaat ttatgtgcag gaatacagca
      721 ttggtaaacc attgtattct gttaaagaga taggtactac tgattacaga ggcaccattc
      781 aaaccttcaa gcctgatgac acaattttta tcacaacaat ttacaactac gacacattag
      841 cttcacgcct gcgcgaacta tcttacctta acaaaggcat ccgcatcaca ttaaccgaca
      901 tgcgcgaaaa agacgagcaa ggcaatgttc gctctgacga gtttttaagt gagttgggct
      961 tgcgcgaatt tgttgaatac attgatgcca atcgcgaaaa gctcatccct gaatgtatgt
     1021 tttttgagag tgataaattc ggaattccaa ttgaagtggc catgcaatac aacacttctt
     1081 ttgcagagaa tctccactcc tatgtaaaca acatcaacac acatgaaggt ggcacacacc
     1141 tttccggttt cagacgtgca ctcacacgta cattaaaagc ctatgccgaa aaagaaggca
     1201 tgcttgccaa attaaaattc gatattgatg gtgacgattt cagagaagga ctaacagccg
     1261 ttatctcagt aaaagttgcc gagccgcagt tcgaaggaca aacaaaaaca aagcttggta
     1321 acaacgaagt tatgggttat gttgatcagg ccgttggcga catgcttgtc cactacctcg
     1381 aaatgcatcc gcgcgaagca aaggctattg tcaacaaagt aatacttgct gctacggcac
     1441 gccatgctgc acgcaaagca cgcgagctgg tgcaacgcaa aaacgtactc actggaacag
     1501 gactacccgg caaattagcc gactgctcag agagcgaccc cggacgatgt gaactcttcc
     1561 ttgtcgaggg agactctgcc ggtggaactg ccaaacaagg ccgcgacaga aatttccaag
     1621 ccatacttcc actacgcgga aaaatcctca acgtagaaaa agcaatggaa tacaaaatct
     1681 acgaaaacga agagataaga aacatcttca ctgcccttgg catctatcgt gacgacaaac
     1741 aagaaggtcg cccgctcata ctcgacaaac tgcgctatca caaaatcgta atcatgaccg
     1801 atgccgatgt tgacggtagc cacatcacca cactcatcct cacattcttc ttccgccaca
     1861 tgaaagaact catagagcag ggctacatct acattgcaac accaccgctc tacctcgtaa
     1921 aaaaaggaaa agaagagcgc tactgctgga
//
LOCUS       DAORQT010000297         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12592_length_1932_cov_4.304742, whole genome shotgun sequence.
ACCESSION   DAORQT010000297 DAORQT010000000
VERSION     DAORQT010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12592_length_1932_cov_4.304742"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..897
                     /locus_tag="PK134_08270"
     CDS             25..897
                     /locus_tag="PK134_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRB38884.1"
                     /translation="MSEDNKLARKALRSSNISTVISISLVLFLIGSLGLLLINAKQLS
                     DYLKENVILTVMMKQEASDADIQNLKKQLEGNAFIKEVSYISSEQAAEQLKQELGEDF
                     NTFLGFNPLLPLMEIKLKPDYTEENNISQLKDFISKNSIVKEVYFQESLLDAINKNIR
                     NISLIILGFSAVLFFIAGALINNTIRLALYAKRMLIKSMKLVGATRNFIRKPFIVRGI
                     LQGLIGAVIANIMILVVFYYAKKQIPEIIAFENPVNLSIVLGIVVFLGIFISGFSTYF
                     AVNKYLRQRTEDIF"
     gene            910..1188
                     /locus_tag="PK134_08275"
     CDS             910..1188
                     /locus_tag="PK134_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRB38885.1"
                     /translation="MAKEKTITKSTTAKSASKPEIQLAFGKINYILMISGIVLILFGY
                     MLMSGGGSDDPKVFNPAIFDTQRLTVAPIMVVLGYALEVLAIVVNAKD"
     gene            1210..>1932
                     /locus_tag="PK134_08280"
     CDS             1210..>1932
                     /locus_tag="PK134_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRB38886.1"
                     /translation="MNTLQAIILAIIEGITEFLPVSSTGHMIIGSSVMGIAELPITKT
                     FTVAIQFGAILSVVFLYWRKFLVIDFNYYKKLLIAFIHAAVLVKLLDDYIDALLENVL
                     VVAAMLLVGGIIFILLDKWFQKTEERGSEELTKDKSAFYIGLFQCIAMIPGVSRSAAT
                     IIGGLAQGLNKKAAAEFSFFLAVPTMFAATLYKMYGFYKLNGGFTAEEIKLFAVGNVV
                     AFIVAAIAIKSFINYLTKHGFKI"
BASE COUNT          602 a          334 c          372 g          624 t
ORIGIN      
        1 gttttaccat ctaaatttgc tgccatgagt gaagataata aactggcaag gaaggcactt
       61 cgcagttcaa atatttcaac cgtaattagt atttcattag tattgtttct gattggttca
      121 ttggggctgt tactcatcaa tgccaaacaa ctttctgatt acttaaaaga aaatgtaatc
      181 ttaacggtga tgatgaagca ggaagcctct gatgccgaca ttcagaatct taaaaaacaa
      241 ttagaaggaa atgcttttat taaagaagta tcatacatca gcagcgaaca ggcggcagag
      301 caattaaaac aagaactggg agaagatttc aatactttct tagggtttaa tccattgttg
      361 ccactgatgg aaataaaact taagcccgac tatactgaag agaataatat ttcgcagcta
      421 aaagatttta tttctaaaaa cagtatagtt aaggaagttt attttcagga gtcgttgctc
      481 gatgccatca ataaaaatat cagaaatatc agcttgatta ttcttggatt cagtgctgtg
      541 ttgttcttta ttgctggtgc attaatcaac aacactatca ggcttgcgct ctatgctaaa
      601 cgtatgctca taaaaagtat gaagttagtt ggggccacaa gaaattttat cagaaaacca
      661 tttattgtaa gaggaatttt gcaaggtctg attggcgcag tcattgccaa tattatgatt
      721 ttagtagtgt tttactatgc caaaaagcag attcctgaaa tcatagcatt tgaaaatccg
      781 gtgaatttga gcatagtttt aggcattgtg gtgtttttag gcatttttat ttcaggattc
      841 agcacctatt ttgcagtaaa taaatatctt cgccaacgaa ctgaagacat cttttaacaa
      901 ttcaaattca tggcaaagga aaaaaccata accaaaagta caacagcaaa gtctgcatca
      961 aagccggaaa tacaattagc ctttggaaaa ataaattaca tcctgatgat ttcgggcata
     1021 gtacttattc tgttcggcta tatgctgatg agtggtggcg gttctgacga tccaaaagtt
     1081 tttaatcctg ccatttttga tacacaacga ttaacagttg cgccaataat ggtagtgctt
     1141 ggctatgccc ttgaagtact tgccattgtt gtgaatgcaa aagattaaaa cactcttcca
     1201 actattttaa tgaatacact gcaggctatc attctggcca tcattgaagg tatcacagag
     1261 tttctgcctg tatcttctac cggacacatg attatcggct cctccgtaat gggcattgcc
     1321 gaacttccca tcactaaaac atttactgtt gccattcagt ttggcgcaat tttatctgtg
     1381 gtatttttat actggcgtaa gtttttagtt atagatttta attattataa aaagttactg
     1441 attgcattta tacatgctgc tgttttagtc aagctgcttg acgactacat tgatgctttg
     1501 ctcgaaaatg tattggtggt tgcagccatg ttgcttgttg gaggtatcat attcattttg
     1561 cttgacaaat ggtttcagaa aacagaagaa cgcggaagcg aagaactcac aaaagataaa
     1621 tctgcttttt atataggatt gtttcagtgc attgccatga ttccgggagt atcgcgctcg
     1681 gcagctacca tcattggcgg cttagctcag ggactcaata aaaaagctgc tgcagaattt
     1741 tcgttcttcc ttgccgtgcc aactatgttt gcagctacac tatataaaat gtatggcttc
     1801 tataaactga atggaggttt caccgcagaa gaaataaaat tatttgctgt gggaaatgtt
     1861 gttgcattta ttgtagctgc cattgccata aaatcattta taaattatct gaccaagcat
     1921 ggatttaaaa tt
//
LOCUS       DAORQT010000298         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12698_length_1919_cov_3.792918, whole genome shotgun sequence.
ACCESSION   DAORQT010000298 DAORQT010000000
VERSION     DAORQT010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12698_length_1919_cov_3.792918"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1058
                     /locus_tag="PK134_08285"
     CDS             <1..>1058
                     /locus_tag="PK134_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRB38887.1"
                     /translation="SMVAFVRDNNMYLKNLSDLSEKMITNDGQKNSIINGALDWVYEE
                     EFSFSQGYQWSNDGMYIAYYRFDESNVKEFTLTYYDSLYPKEEKYKYPKAGENNSVVE
                     IFVYDLASGKSVRMQTGDEKDQYIPRIKWTESKGQLCVLRMNRHQNNLDYLLYNAVTG
                     KASLLMNETNNTFIEITDNLVFLKDGNHFIYSSEKCGSNQLYLRNVNNGSEKMLTNGG
                     EITAFYGYDVKTKTCFYQVADPTPMERVVYSVDLNQKKKLLSPANGTADASFSSDFSY
                     SVITHSTIDSPFNCYVQDGKAKKIRVLENNERVIKNLESFVVSKPEFFKFTNSEKISL
                     NGWMITPPGFKANENKKY"
     assembly_gap    1059..1079
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1080..1713
                     /locus_tag="PK134_08290"
     CDS             <1080..1713
                     /locus_tag="PK134_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRB38888.1"
                     /translation="GRQTVLDKWDGSRYLWHQMLAQDGFIVVSVDNRGTPGRGKEFVN
                     SIYKNMGHNETIDQIEFAKYLRTLSYVDSTRIGVHGWSYGGYMTSLLMTKGADYFKVG
                     VAVAPVINWRYYDSIYTERYLSLPAENPKGYDDNSPIFFADKLKGKYLLIHGLTDDNV
                     HFQNSAEMVKALVKSKKQFDTFYYPDQNHSISQSRWHLYEMMTEYFKKNL"
BASE COUNT          664 a          255 c          380 g          599 t
ORIGIN      
        1 gtagtatggt tgcatttgtt cgtgataata atatgtatct aaaaaatctt tctgacctaa
       61 gtgaaaagat gattactaac gatggacaga aaaattctat tatcaatggt gcattagact
      121 gggtttacga agaggaattt tctttcagtc agggttatca atggtcaaac gatggaatgt
      181 atatagcata ctacaggttt gacgaaagca acgttaaaga atttacactt acatattacg
      241 actcacttta tccgaaagaa gagaaatata aatatcctaa agccggtgaa aataattctg
      301 ttgttgaaat atttgtttat gatttagctt caggcaaaag tgttagaatg caaaccggag
      361 atgaaaaaga tcaatacatt ccaagaataa agtggacaga aagtaaagga cagttatgtg
      421 tattgagaat gaaccgacat cagaataatt tggattattt gttgtataat gctgtaacag
      481 gaaaggcttc attacttatg aatgaaacta ataatacatt tattgaaata acagataact
      541 tagtttttct aaaagatgga aatcatttta tttatagtag tgaaaagtgc ggaagcaacc
      601 aactttattt gcgaaatgta aataatggct ctgaaaaaat gctcactaac ggtggcgaga
      661 taactgcgtt ttatggatat gatgtgaaaa caaagacatg tttttatcag gttgcagacc
      721 caactccaat ggaaagagtg gtgtattctg ttgacttgaa tcaaaagaag aagttgttat
      781 cacctgctaa tggaacagca gatgcctcat tcagtagtga tttttcctat agcgttatta
      841 cccatagcac catagatagt ccttttaatt gttatgttca ggatggcaaa gcaaagaaaa
      901 taagagtgtt ggagaataat gaaagggtaa tcaaaaatct ggaatcgttt gttgtttcca
      961 agcctgagtt ttttaagttt acaaattcag agaaaattag tttaaatgga tggatgataa
     1021 caccaccggg ttttaaagcc aatgaaaata aaaagtatnn nnnnnnnnnn nnnnnnnnng
     1081 ggcagacaaa ctgtgctcga taaatgggat gggtcaagat acttgtggca tcagatgctt
     1141 gcacaagatg gatttattgt tgtatcagtt gacaacagag gaacacctgg tagaggcaaa
     1201 gaatttgtca atagtattta taaaaacatg ggtcataatg aaacaataga tcagattgaa
     1261 tttgcaaagt atcttcgtac attatcttat gttgattcaa cccgcattgg tgttcatggc
     1321 tggagttatg gtggctatat gacatcttta cttatgacaa agggtgctga ttattttaaa
     1381 gttggtgtag ctgtagcgcc tgttatcaat tggcgttatt acgattctat ttatactgaa
     1441 agatatttgt cattacctgc agagaatccc aagggatatg atgataactc acctatattt
     1501 tttgctgata aattgaaagg taaatatttg ctcatacacg gattaactga tgataatgta
     1561 cattttcaga acagtgcaga aatggtaaag gcattagtga aaagtaaaaa gcagtttgat
     1621 actttttact accctgatca gaaccattcc attagccaat ctcgttggca tttgtatgaa
     1681 atgatgacag aatattttaa gaaaaattta taacgttcct tatagtttta cctcagttaa
     1741 aatcaacctt aaaaattgtg attgtaatac ttaatattaa aggtctttgt ggaattcttg
     1801 ataaagagat actcatcaaa cgtggacagg atttacgcag ttttgaaatc ttgccttctg
     1861 cttttatagc tgtaaaagat gggttaatag aaagctatgg taccatgact gaatgggaa
//
LOCUS       DAORQT010000299         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12737_length_1915_cov_21.106452, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000299 DAORQT010000000
VERSION     DAORQT010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12737_length_1915_cov_21.106452"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1767)
                     /locus_tag="PK134_08295"
     CDS             complement(289..1767)
                     /locus_tag="PK134_08295"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38889.1"
                     /translation="MAATLCCCHFFCFSQNKNSVWCFGDSAGIDFGSGVPVTFQSGMD
                     SRGSCVSISDNNGNLLFYAATMPYSGSNPAFSTIVFNSTNSIMANGDSIFGKGWYQEL
                     VIVPNPANDSTYYLFSIGSTTTFGLCYSIIDMRLNNGLGAVTVKNIQLQSFQQVDCLN
                     AVKHGNGRDWWVLFRKAGSLSNGNNDWYSYLITPDSIQNVSVQSIGSLNKTNSGHTNL
                     IGLTELYDFDRCSGLLSNLVTIEPEALPSVPYLFSCAFSPDASKLYVSTADQPTWLFQ
                     YDLNASNITLSKDTLWTISSPQYAGGGLKLAPDNKIYLASIYYNGVQFPYPYQDTVYN
                     MYNMNLSVINQPDSLGSACDFQPYSFYLGGKRTYLGLPNNPDYTLPAISGSPCDTLTG
                     IAPLATSAKGAELFVTYMASWQKLFVNAQHLKGKNITLTLYDVTGKLIYNTARVLSPS
                     GVGGSAGGYFTQDINCSALAKGMYIVSLQTEKERLVNKFIVE"
BASE COUNT          617 a          373 c          329 g          596 t
ORIGIN      
        1 atttattaat agacagctgt ttgtttcctt aacttaatga cattggactt gcgccagtgg
       61 gggaaaatat caatccagcc aatggttgta aatgttacaa aatataaacc gcccggaaaa
      121 gttttataaa cttcagacat ttgttgtttg catatatgca aaaatgataa tttttagatc
      181 agtatatgat tgaagtaaaa cttccaaaca caaaacccac gaagcacagc ttcgcgggta
      241 cggggatgct gcgcttatgt atttgagtaa tttcatttaa aacaaaagtt attcaacaat
      301 aaatttattt accaatcttt ctttttctgt ttgcaacgaa acaatgtaca tgccttttgc
      361 aagtgcgctg caattaatat cctgtgtaaa ataaccgcct gcgctacccc caacccctga
      421 aggggagagc actcgcgcag tgttataaat tagcttgcct gtaacatcat atagggttag
      481 tgttatattt ttccctttta aatgctgcgc atttacaaac aatttttgcc atgatgccat
      541 gtaggtaaca aacaactccg ctccctttgc tgatgttgca agaggtgcta tacccgttaa
      601 tgtatcacag ggactgcctg atattgcagg cagtgtataa tcaggattat taggcaaacc
      661 taaatacgtt cttttaccgc ccagataaaa actgtacggc tgaaaatcgc aagcacttcc
      721 taaactatca ggttgattta ttactgacaa gttcatattg tacatgttgt aaacagtatc
      781 ctgatatgga taaggaaatt gaactccgtt atagtatatg ctggctaaat aaattttatt
      841 gtctggagct aattttaatc cgccacctgc atattgtggg ctgcttatag tccaaagcgt
      901 gtcttttgat aaagtaatat tagatgcatt caaatcgtac tgaaaaagcc atgtcggttg
      961 gtcagcagtg cttacataaa gtttgcttgc atcgggtgag aatgcgcaac taaataaata
     1021 tggcactgat ggcaacgctt ccggttcaat ggtaacaaga ttgcttaata atcctgagca
     1081 acggtcaaaa tcatatagct ctgttaaacc aatcaaattg gtatgtccag agttagtttt
     1141 gtttaatgaa cctattgact gaacggaaac attctgaatg ctgtcaggtg ttattaaata
     1201 agaataccag tcattgttgc catttgataa agaacctgct tttctgaata atacccacca
     1261 gtctcttcca ttgccatgtt taacagcatt caggcaatcc acctgctgaa aactttgcaa
     1321 ttgaatattt ttaactgtaa ctgcacccaa tccattattt agccgcatat caataatact
     1381 atagcacaaa ccaaaagtgg tagtacttcc aatagaaaat aaataatagg tgctgtcatt
     1441 ggcaggatta ggcactatta ccaattcctg ataccatcct ttgccaaata tagaatcacc
     1501 attagccatt attgaatttg tggagttaaa aacaatggtt gaaaatgcgg gatttgaacc
     1561 tgaataaggc attgttgctg cataaaaaag taaattcccg ttattgtctg aaatgctgac
     1621 acagcttcct ctactatcca taccggattg aaaagttaca ggtactcccg aaccaaaatc
     1681 tattcccgca ctatcaccaa agcaccaaac agaattcttg ttttgtgaaa agcaaaaaaa
     1741 atggcagcaa cagagtgttg ctgccattag taaacatttt aaattattta ataaccacaa
     1801 accgcttttt aaagtattcg cctgtttcat tctttattct aatattatac actccgtcag
     1861 aaagcgaaga tacatctatt acaccactgc tgctattatt ttgctttttc aataa
//
LOCUS       DAORQT010000300         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12776_length_1911_cov_5.260237, whole genome shotgun sequence.
ACCESSION   DAORQT010000300 DAORQT010000000
VERSION     DAORQT010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12776_length_1911_cov_5.260237"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..>1911)
                     /locus_tag="PK134_08300"
     CDS             complement(139..>1911)
                     /locus_tag="PK134_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38890.1"
                     /translation="LLAIILMSYSTQATHLFGGEITWKCLGTGKYQFTLKIYRDCMGF
                     PFSPPAALDVYGNPNITSIPINPAYTVYNDLTPTSCGFDCNNPQPGAVIECIMKTDPI
                     FLVGIPPPATGWVIAYDDCCRQPLNNLGGGGQGFTIRATIYSYNNLSSAPCFDSSPFF
                     AERPYAFTCIGYPFTYNPNAIDADGDSLVYEWAYCLEQPTFTPPAAFNAPSITISFNP
                     PPYTRLNQIPGNPVLDSHSGELSFFVDTASATIGSFGSCIKVSAYRCGKKIAEIFRDC
                     AFVLTNNCYVQPGPPAINLPPVYNLPFNSGTASDTVVTAGDTVRFSLHFTDIQQNMGA
                     FPTQFITINASGSYFGTNFTIDTAGCPSPPCATLNKTLPSSSPIVNSVNFQWVPNCTL
                     INLNTCNNNPENTYYFVFDASDNFCPVPAHAYVTAKITVAGPSIIQSNDTLYVNYPGA
                     ISYQWYNGGNLIPGATNSFYIVTVPGLYSCQISNTTGCNISSRLMQVTQTSIAETENL
                     FSWDIIPNPASDKVTIAINSPVANEGKIVLTDISGRIIEQQQIQLLKGRNTFNINTHS
                     FARGMYQLCLQSGKTKLMEKLLLK"
BASE COUNT          620 a          327 c          382 g          582 t
ORIGIN      
        1 ggatagatat ttgaagtaat aaaaaagcgg gacaagcccg cttttttgtt ttgcattgga
       61 gagtttagaa atctttatag ctatatattt tgtcttacat tattaatcaa ctccttttca
      121 gattcaatca actcaacttc atttcaacag taatttttcc atcaactttg tttttccgga
      181 ttgcaagcac aactgataca tacctcttgc aaaagaatgt gtgttgatgt tgaaagtatt
      241 tcggcctttt aaaagttgaa tctgttgctg ctctattata cgaccggaaa tatcggtaag
      301 cacaatttta ccctcattag ctaccggtga gttaatagca atagtaactt tatcagaagc
      361 cggattggga ataatgtccc aggaaaataa attttctgtt tcagcaatag atgtttgtgt
      421 tacctgcatt aatcttgaac tgatattaca accagttgtg ttacttattt gacatgaata
      481 caagcccgga accgtaacta tataaaaaga attggttgct cccggaatga gattaccacc
      541 attataccat tgatatgaaa tagcacccgg atagtttaca tacagtgtat cgtttgactg
      601 tataattgat gggcctgcaa cagtaatttt agccgttaca taggcatgag caggtacagg
      661 acaaaagtta tctgatgcat caaaaacaaa atagtatgta ttttccggat tgttattgca
      721 tgtattgagg ttgattaatg tacaattcgg cacccattga aaattaacgg aatttactat
      781 tggtgatgat gatggtaaag tcttgtttag tgttgcacat ggtggagatg gacaacctgc
      841 agtatcaata gtaaagttgg taccaaaata tgaaccgctt gcattgatgg ttataaattg
      901 agtaggaaaa gcacccatgt tttgttgaat atccgtaaaa tgcaacgaga atctaacagt
      961 gtcgcctgca gttacaactg tatcagaagc agtacctgaa ttaaacggta agttatatac
     1021 aggcggcaga tttattgcag gaggccccgg ttgtacatag cagttgttag ttaagacaaa
     1081 ggcacagtct ctaaaaattt cagcaatttt ttttccgcag cgataagcag aaacttttat
     1141 acaactccca aaacttccta ttgttgcact tgcagtgtca acaaaaaaac tcaattcgcc
     1201 tgaatgacta tctaatactg ggttgccggg aatttgattt aatcgtgtgt atggtggagg
     1261 attaaatgaa atggtaatac taggagcatt aaaggctgca ggtggagtaa aggtgggttg
     1321 ttctaaacag taagcccatt cgtaaaccaa agaatctcca tctgcgtcaa tggcatttgg
     1381 attataagta aatgggtaac caatacaagt aaaagcataa ggtcgttctg caaaaaaagg
     1441 tgatgaatca aagcatggcg cagaactcaa attgttataa gaataaatgg tggcacgtat
     1501 cgtaaaacct tgtccaccac cacctaaatt atttaatggt tgtctgcagc aatcatcata
     1561 agcaatcacc catcctgtag caggaggggg tattccaaca agaaaaatcg ggtcggtttt
     1621 cattatacat tcaattacag cacctggttg tggattgttg cagtcaaaac cacaacttgt
     1681 tggtgttaaa tcgttataaa ccgtatatgc agggttaata ggtatggaag taatattagg
     1741 gtttccataa acatctaatg ctgcaggagg actaaatgga aatcccatac aatcgcgata
     1801 tattttgagt gtaaattgat atttaccggt cccaagacat ttccatgtga tttccccacc
     1861 gaataaatgc gttgcctgtg ttgaataact catcaagatt atagctaata a
//
LOCUS       DAORQT010000301         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12954_length_1892_cov_4.400653, whole genome shotgun sequence.
ACCESSION   DAORQT010000301 DAORQT010000000
VERSION     DAORQT010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_1892_cov_4.400653"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK134_08305"
     CDS             complement(<1..316)
                     /locus_tag="PK134_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HRB38891.1"
                     /translation="MMKKLLTLLFAVVILPVVALSQSPALVGYWHNWDITAAPYIMLD
                     SVDSRYNIIDIAFAVPQAGTDYQMEFIPEQVTPAAFATQVQNLQNQGRKVIISMGGAT
                     APV"
     gene            complement(373..906)
                     /locus_tag="PK134_08310"
     CDS             complement(373..906)
                     /locus_tag="PK134_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB38892.1"
                     /translation="MRKIHKIAIVGPESTGKSLLSEQLSNHYNTAWVPEVARDYLHKL
                     NRPYTFDDLSVIAKLQLKEEERLSKTAKHFLFCDTNLIVIKVWSDFKYGRTEKWIVDA
                     IQKSDYSLHLLTDIDLPWEDDPLREHPHFRSQLFELYLQELNSFGLEYQTVRGIGEDR
                     IKNALNHIEKLEPNLIR"
     gene            complement(890..1162)
                     /gene="pnuC"
                     /locus_tag="PK134_08315"
     CDS             complement(890..1162)
                     /gene="pnuC"
                     /locus_tag="PK134_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRB38893.1"
                     /translation="MGGWFSHYTDAHLPYWDALATSMCFAAQYLMAEKKIENWLVWID
                     ANILYIGIYLYKGLPLYAILFSIYLIMAIVGYFKWQNHLIRHEENS"
     gene            complement(1072..1488)
                     /locus_tag="PK134_08320"
     CDS             complement(1072..1488)
                     /locus_tag="PK134_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HRB38894.1"
                     /translation="MDLQTIMFLNIPLIEWIGFLFGVVGIYLGIKEKIAYFPISLINV
                     IVSLYIFYHQSLYSDVVQQGVYILLLSYGWYRWIKGSQNTEMPVTNASKKLIFILLGG
                     IILLTVAWVVGSVIILMHIYRIGMHWQQACVLQHNI"
     gene            complement(1492..>1892)
                     /locus_tag="PK134_08325"
     CDS             complement(1492..>1892)
                     /locus_tag="PK134_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRB38895.1"
                     /translation="LEAIPTGYILIDSGNVTTVQYVSNTKPIPHDHSELAVSTAVAGQ
                     LLGMQVIYLEAGSGARRTVSGQMIELVKKNTGLPLIVGGGIRTAEKAIEIYNSGADII
                     VVGNAIEDNPELMIEIAKAKTTINQTLNAL"
BASE COUNT          610 a          385 c          297 g          600 t
ORIGIN      
        1 aaaccggtgc tgtggcacct cccatactga taattacctt gcgtccctga ttctgcaaat
       61 tctgaacctg cgtagcaaat gctgcaggag taacctgctc gggaataaat tccatctgat
      121 agtcggtgcc tgcctgaggc actgcaaatg caatgtcaat aatattataa cgtgaatcaa
      181 cactatcaag cataatgtat ggtgctgctg taatatccca attatgccag taaccaacta
      241 atgcaggcga ctgcgacaat gccactaccg gtaagataac gactgcaaat aaaagtgtaa
      301 gtagtttttt catcattatt tttaattaaa gaaattctat cagatgcgaa aataatcaat
      361 acagatttct attcacctta ttaaattcgg ttcaagtttt tcaatatgat tcaatgcatt
      421 ttttatcctg tcctctccta ttcctcgtac agtttgatac tctaatccaa agctattcaa
      481 ttcttgtaaa tataattcaa acagttgact tctgaaatgc ggatgttctc ttaaaggatc
      541 atcttcccaa ggtaaatcaa tatctgtaag caaatgtaat gaatagtcac tcttctgaat
      601 tgcatcaaca atccactttt ctgttcgtcc atatttaaag tcgctccaaa cttttatcac
      661 gattaaattg gtatcgcaaa acaaaaagtg ttttgcagtt tttgacaatc tctcttcttc
      721 ctttaactgc aattttgcta ttaccgacaa atcgtcaaag gtgtatggac gatttaattt
      781 atgtaaatag tcacgtgcaa cttcaggcac ccaggcagta ttataatgat tggataactg
      841 ttcacttaat aaactctttc ctgttgactc aggcccaacg atggcaatct tatgaatttt
      901 cctcatgtct gatgagatgg ttctgccatt taaaatatcc tacaatagcc atgataagat
      961 aaatagaaaa caaaattgca tagagcggca gacctttata aagatatatt cctatatata
     1021 aaatattcgc atctatccaa acaagccagt tctcaatttt cttttcggcc attaaatatt
     1081 gtgctgcaaa acacatgctt gttgccaatg catcccaata cggtaaatgt gcatcagtat
     1141 aatgactgaa ccaaccaccc atgcaacagt aagtaaaatg attccaccaa gaagaatgaa
     1201 aatgagtttt tttgaagcgt tggtaacagg catttctgtg ttttgactgc ctttaatcca
     1261 gcgataccaa ccataggaaa gcaatagaat gtaaacacct tgttgaacaa catcactgta
     1321 taatgactga tgataaaaaa tataaagtga tacaattaca ttaattaaac taatcggaaa
     1381 gtatgcaatc ttttctttta ttcctaaata aattccaacc actccaaaca aaaagcctat
     1441 ccattcaatc aaaggaatat ttaaaaacat aatagtttgt aagtccacag gctacaatgc
     1501 gtttaaggtt tggtttatag ttgtttttgc ttttgcaatt tcaatcataa gttcgggatt
     1561 atcttcaatg gcatttccta ccacaataat atcggcacct gaattataaa tctcaattgc
     1621 tttttcggca gtacgaatac caccaccaac tatcaaaggc agacctgtat tttttttaac
     1681 caattcaatc atctgtccgg atacggttct tctggctcca cttccggctt caagataaat
     1741 tacctgcatg cctaaaagtt gtccggcaac agctgtagaa actgcaagtt cgctatgatc
     1801 gtggggaatg ggttttgtat tgcttacata ctgcactgtt gttacattac cactgtcaat
     1861 aagaatgtat cctgtaggta tagcctcaag cc
//
LOCUS       DAORQT010000302         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_12975_length_1889_cov_6.472192, whole genome shotgun sequence.
ACCESSION   DAORQT010000302 DAORQT010000000
VERSION     DAORQT010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12975_length_1889_cov_6.472192"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /gene="rseP"
                     /locus_tag="PK134_08330"
     CDS             complement(<1..1270)
                     /gene="rseP"
                     /locus_tag="PK134_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRB38896.1"
                     /translation="MSGLIMAAQLILGLSILVTLHELGHFLAARMFGIKVEKFYLFFD
                     AWGIKLFSFKKGDCEYGIGWLPFGGYVKIAGMVDESMDKDQLAQPAQPWEFRSKPAWQ
                     RLIVMVAGVVMNVILGIAIFSMSLLHYKGSYLKNDAVTHGIVAYKLGEEIGLRTGDKV
                     VAIDGKSFDRFDDLLSTRVLFGATLTVIRNGEKVEIEVPDDFYKKTGPNARSLFLGAG
                     HLTYVVKEVVVGENAFKAGVENDDIITEVDKQKITGEDFMQRYFADKKGETALLSIIR
                     AGKPMDITVNISSKGTIGVRYNGDVVVPADYVKTPYTLASAFAFGTSDAFETLVSNAK
                     GFKKLFKGEEKFQESVQGPIGIAKIYGGTWDWPHFWVLTGLLSMILAFMNILPIPALD
                     GGHVLFLLIESVTRRKFSDAFMEKTQVVGMV"
     gene            complement(1286..>1889)
                     /locus_tag="PK134_08335"
     CDS             complement(1286..>1889)
                     /locus_tag="PK134_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRB38897.1"
                     /translation="VTLAQALKHPNWSMGAKITVDSATMMNKGLEMIEAKWLFGLQPE
                     QIDVIVHPQSIIHSVVQFTDGSMKAQMGLPDMKLPILYALSFPHRLKTDFKRFCFLDY
                     PQLTFEKPDTEVFPALHLAEEAMKKDGNMPCILNAANEVAVAAFLKEKISFFQIPGIT
                     EQLMSKITYIEKPSLEDLIATDKETRMQTELLLTKKINFA"
BASE COUNT          580 a          409 c          313 g          587 t
ORIGIN      
        1 acaccattcc aacaacctga gttttttcca taaaggcatc actgaatttt ctgcgtgtga
       61 cagattcaat gagcagaaat aaaacatgcc caccatcgag tgcaggaatt ggaagtatat
      121 tcataaaagc aagaatcata gagagtaatc cggtaagtac ccaaaaatgt ggccagtccc
      181 aggtgccgcc atatattttt gcaataccaa taggcccctg aacagattcc tgaaattttt
      241 cttctccttt aaaaagtttc ttaaagcctt ttgcattact cactaatgtt tcaaatgcat
      301 ctgaggtacc aaatgcaaat gccgatgcca atgtgtaagg agttttaaca taatccgcag
      361 gaacaacaac atcaccatta tatcgtacgc caattgttcc tttagaactt atgtttaccg
      421 taatgtccat tggtttaccg gcacgaataa ttgagagcaa tgcagtttca ccttttttat
      481 cggcaaagta acgctgcata aaatcttcac cggtaatttt ttgcttatca acttctgtaa
      541 tgatgtcatc gttttctact cctgctttaa aagcattttc gccaacaaca acttctttaa
      601 caacataggt cagatgtcct gctccaagaa aaagggaacg tgcattaggt cctgtttttt
      661 tataaaaatc gtctggcact tcaatttcta ctttttcacc atttcttata acagttaatg
      721 tagcaccaaa caatacgcgt gttgaaagca ggtcatcaaa acgatcgaaa gatttaccat
      781 caatagcaac aactttatca cctgtgcgca agccaatttc ttctcccagc ttataagcaa
      841 ctatgccatg tgtaacagca tcgtttttca gatagctgcc tttgtagtgc aacaaactca
      901 ttgaaaaaat tgctatacca agaatgacat tcattacaac acctgcaacc attacaatta
      961 atctttgcca tgcaggttta cttctgaatt cccatggttg tgccggctgt gctaattgat
     1021 ctttatccat gctttcatca accatacctg caatttttac atagccgcca aaaggaagcc
     1081 atcctatacc atactcacag tcgccttttt taaagctaaa caattttatt ccccatgcat
     1141 caaaaaacag gtagaatttt tcgaccttga taccaaacat tcttgcggca agaaaatgtc
     1201 ctagctcatg cagggttacc aatattgaga ggcccagtat caactgagct gccataatca
     1261 atccactcat cagattttaa aaattttatg cgaagttaat tttttttgtc aatagtaatt
     1321 ctgtttgcat tcgtgtttcc ttatcggttg caatcaggtc ttcaagtgat ggtttttcaa
     1381 tgtaagtaat tttcgacatg agttgttctg taataccggg aatctgaaag aaacttattt
     1441 tctctttgag aaacgcagca acggcaactt cgtttgctgc attgagaata caaggcatgt
     1501 ttccatcttt tttcatagct tcttcagcca aatgtaaggc aggaaaaact tctgtatccg
     1561 gtttttcaaa agttagttga ggatagtcaa gaaaacagaa tctcttgaaa tcggtcttta
     1621 aacgatgcgg aaaactcaat gcataaagta tgggcagttt catatcaggc aaacccattt
     1681 gtgctttcat actgccatca gtaaattgca ctacagaatg aatgatagat tgcggatgca
     1741 caattacatc aatctgctcc ggttgtaaac caaacaacca tttggcttca atcatctcaa
     1801 gacctttatt catcatggtg gcagagtcaa cagtaatttt tgcacccatg ctccagtttg
     1861 gatgtttcaa tgcctgagcc agagttacc
//
LOCUS       DAORQT010000303         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13019_length_1884_cov_4.908693, whole genome shotgun sequence.
ACCESSION   DAORQT010000303 DAORQT010000000
VERSION     DAORQT010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13019_length_1884_cov_4.908693"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1029)
                     /locus_tag="PK134_08340"
     CDS             complement(174..>1029)
                     /locus_tag="PK134_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB38898.1"
                     /translation="RSENTNYSTQNKELTSSVADLKREKEKLETDTTTLGTAQRRLTS
                     LYNELNDSYEKLLRNNDRMMKDKNDETKKVIGQLQMTQEELIKKQDELTAKEKKLNEL
                     TEELKKRESKVNELESVLNKKDEDVNALRKAVSDALVGFENNGLTITKKNGKVYVSME
                     EKLLFASGSTVVDKKGEEALKELGKVLEKNKDINVLIEGHTDNVPISGGTMKDNWDLS
                     VLRATSVVRILIKNSNIDPTRLTPAGRGPYLPVDTGNTAEARRKNRRIEIILTPKLDE
                     LFKVIGEN"
     assembly_gap    1030..1065
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1319..>1884
                     /locus_tag="PK134_08345"
     CDS             1319..>1884
                     /locus_tag="PK134_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38899.1"
                     /translation="MATLNDNWITEAHIDFEYKKYMLLAYLQHVSESFTENKLYPPLS
                     ELMKHYRNVTALRDNKKQFYDSFPERLSSADFNNFKLMYEKIVADDSLMQEVESIILF
                     SIPQFEKHISEGKKIYDFIESKIKVSPVGIIPLQINEGYFFLSTTQNRETRVYEYQIT
                     IFENPDEKYRALCTQYICSYEKNLSQNF"
BASE COUNT          556 a          348 c          304 g          640 t
ORIGIN      
        1 atgttattgg ctgaagtaaa gaaagaacct gtaagtaatg tcaaccaact acacattaaa
       61 tacaaagttg tctgtgcaaa agtaactgcc cttcagacat tcattttagt aaatgatgaa
      121 gggcagccta aaattatttt tcagaatgtt gaaaatatct gaaccgctaa aaattaattc
      181 tctcctataa ctttaaaaag ttcgtcaagt tttggggtta agataatttc tatgcgtctg
      241 ttttttctgc gtgcctcagc agtgttaccg gtatcaacag gcaaataagg tcctctgcct
      301 gcaggagtta atcttgtagg gtcaatattg gagtttttaa taagaatacg aaccacactt
      361 gtagcacgca acacacttaa atcccagttg tctttcatag ttcctcccga aattggcaca
      421 ttgtcagtat gaccttcaat caacacgttg atgtctttgt ttttttcaag caccttgccc
      481 aattccttca atgcctcctc acctttttta tcaaccacgg tgcttcctga agcaaacaac
      541 aatttctcct ccatgctcac atagacttta ccattctttt tagtgatggt aagtccgtta
      601 ttttcaaaac ctacaagggc atcactcaca gcttttctca atgcattaac atcttcatct
      661 tttttattca atacagactc cagctcatta acttttgatt cgcgtttttt aagttcttct
      721 gtcaactcat tcagcttttt ctcttttgcg gtaagttcat cctgtttttt tatcagctct
      781 tcctgtgtca tctgtaactg accgattact tttttagttt catcattctt atctttcatc
      841 atcctgtcgt tattgcgaag gagcttttca tacgagtcgt ttagttcatt gtaaagtgat
      901 gtcagcctgc gttgtgcagt tcctaaggta gtggtgtctg tctctaattt ttctttttcg
      961 cgttttaaat ctgcaacaga agaagtgagc tccttatttt gggtagaata attggtgttt
     1021 tcactacgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcatc tcatcatatt
     1081 gtcttgccgg aacacatgaa gccaatgcag taatgactgc aatcacacct attaaattta
     1141 aaaatttctt gttcattttc gttgtgttta aatttattga aactttgttt ctgaatatta
     1201 agtaaaatta taatatagtg cccaaaatta agcgtttgtc gctttgagtt tttgcaagaa
     1261 tagatgcatc aggtataatt tttgaacaat taaaacagac aaaaaaagtt taaatgttat
     1321 ggcaacacta aatgacaact ggattacaga agcacatata gactttgagt acaaaaagta
     1381 tatgctgcta gcgtatttac aacatgtttc agagagcttt actgaaaaca aactctatcc
     1441 gccattaagc gaactgatga aacactatcg taatgtaacg gcattgcgtg ataataaaaa
     1501 acaattttat gatagttttc cggaacggct ttcttctgcc gattttaaca actttaaact
     1561 catgtacgaa aaaattgttg cagatgacag cctgatgcag gaagttgaga gtatcattct
     1621 attcagtatt cctcagtttg aaaaacatat ttctgagggt aaaaaaattt atgattttat
     1681 agaatcgaaa attaaagtca gtccggttgg tattattcca ttgcagatta atgagggtta
     1741 tttctttctt tcaaccacac aaaaccgcga aacaagagtg tatgaatatc agataaccat
     1801 ttttgaaaat ccggatgaga agtatcgcgc actttgcaca cagtatattt gttcgtacga
     1861 aaaaaacctg tcgcagaatt tcga
//
LOCUS       DAORQT010000304         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13149_length_1871_cov_6.634361, whole genome shotgun sequence.
ACCESSION   DAORQT010000304 DAORQT010000000
VERSION     DAORQT010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13149_length_1871_cov_6.634361"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PK134_08350"
     CDS             <1..1638
                     /locus_tag="PK134_08350"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB38900.1"
                     /translation="MTYYTALDNPPKGFPLTQFSMLEAEDVGLYKFDILSQRGLGKIK
                     DAVAVIEKNRQVKVDIHDIKKFKEDEGVRQKLQQANLMGCFYVESPAMRMLLKKLRAK
                     TYLDLVAASSIIRPGVAQSGMMREYILRFHEESRRTYIHPMMEELMRETFGVMVYQED
                     VIKVAHYFAGLTLTEADMLRRGMSGKYRGRAEFEKVKDAFFNGCRKKGHADKIAEEVW
                     RQIESFAGYAFSKGHSASYAVESYQCMFLKTYFPLEYMVACVNNGGGFYAAEFYVHEA
                     RMCGGTIHAPDVNKSFYETSLYGKNIYAGFHLVHELEMLTVTNILKARAAEGQFISLA
                     DFMQRVEISLEQLRLLIRVNALRFTGRTKQQLLWDIHEIMGHDKKTNTEKQLFEAEHK
                     HFVLPELHYSKIDDVWDEIELLGFPLCSPFDLIKQKTNVTEGIVIHTAAELSAYCGKI
                     ISIAGYFVTRKPTRTKHGQPMAFGTFLDSKGQWIDTTHFPDVVKQYPFRGRGCYRITG
                     KVVQEFGFYSIDVISLYLLEYKTRYAVVEEDKVHVNE"
BASE COUNT          564 a          342 c          441 g          524 t
ORIGIN      
        1 atgacgtatt atacggctct cgataatccg cctaaaggtt ttccgcttac gcagttcagt
       61 atgctcgaag cagaagatgt aggtctttat aagtttgata ttttgtcgca gcgcggactg
      121 ggaaaaatta aagatgctgt tgctgtcatt gaaaaaaacc gacaggtaaa agttgatatt
      181 catgacataa aaaagtttaa agaagatgag ggcgtcaggc aaaaactgca gcaggcaaat
      241 cttatgggat gcttttatgt agaaagtcct gccatgcgca tgttactgaa aaaactacgt
      301 gcaaaaacct atctcgattt ggtggcagca agttcaatca tccgtccggg ggtggcacaa
      361 agcggcatga tgcgcgaata tattttgcgc tttcatgagg agagccgcag aacctacatt
      421 catccgatga tggaggagtt gatgcgcgaa acttttgggg tgatggtata tcaggaagat
      481 gtgattaagg tagcacatta ttttgccgga cttacactca cagaagccga catgctgcgc
      541 agaggcatga gtggaaaata tcggggacgt gcagagtttg aaaaagtgaa agatgctttt
      601 ttcaatggtt gccgcaagaa aggacatgcc gataagatag cagaagaagt gtggcggcag
      661 attgaatcgt ttgcaggcta tgctttcagc aaaggccatt cggcatcgta tgccgttgag
      721 agctatcagt gcatgttttt aaagacctat tttccacttg aatatatggt ggcatgtgta
      781 aacaacggtg gtgggtttta cgcagctgaa ttttatgtac atgaagcgcg tatgtgtgga
      841 ggcaccatac atgcacctga cgttaataag agtttttatg aaacatcgct ctatgggaaa
      901 aatatttatg caggctttca tttagtgcat gaacttgaaa tgttgacggt aacaaatatt
      961 ttaaaagcca gagcagcaga agggcagttt atttcgcttg cagattttat gcagcgcgtt
     1021 gaaatttctc tcgaacaatt gcggctgctc attcgtgtta atgctttgcg ttttacagga
     1081 agaaccaagc aacaactgct gtgggatatt catgaaataa tggggcatga taaaaaaaca
     1141 aatacagaaa aacagttgtt tgaagccgaa cacaaacatt ttgtccttcc ggaattgcat
     1201 tacagcaaaa ttgatgatgt gtgggacgaa atagaattgt tgggatttcc gctgtgctca
     1261 ccatttgatt tgataaaaca aaaaacgaat gtaacggaag gaatagtgat tcatactgct
     1321 gcagaactgt cggcatactg cggaaaaata atttccattg ccggttattt tgttacccgc
     1381 aagccaacac gaaccaagca tgggcagcct atggcattcg gtacttttct cgacagcaaa
     1441 ggacaatgga tagacactac acattttccc gatgtggtaa aacaatatcc ttttcgcgga
     1501 cgcggatgct accgcattac cggaaaggta gtgcaggaat ttggttttta cagcattgat
     1561 gtcatcagcc tttatctgct tgaatataaa accagatatg ctgtggtgga ggaggataaa
     1621 gttcatgtaa atgaatagga gtaaacaata aaaaaagtaa aacttcccta agtaggcgca
     1681 agcgtctttc gctcgtgctt ttatccttgt ggcctctatc cgtccgatag ctatcggact
     1741 tcatagttca accagcttta aatcggtaaa ttgattagca ctactatact ccatacccgc
     1801 gaagttgcac ttcgtgggtt tttgtgcaaa gaagttttac ttctgaaacc ataccgtttc
     1861 ctttattttt a
//
LOCUS       DAORQT010000305         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13258_length_1861_cov_6.102990, whole genome shotgun sequence.
ACCESSION   DAORQT010000305 DAORQT010000000
VERSION     DAORQT010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13258_length_1861_cov_6.102990"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PK134_08355"
     CDS             complement(<1..915)
                     /locus_tag="PK134_08355"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRB38901.1"
                     /translation="MFKRAFLINLFFAFCFSSFASDSLQVDTNTIYVRQIVVTGNKVT
                     RYSVIQRELAFSQGQTMTVGELNEKITKSRENLLNISLFNFVNINKQLIGNDVYVIID
                     VVERWYIFPVPIFEVVDRNFWEWWRDKNLKKANYGFYLNWENFRGRRENVRLLLRYGY
                     SQRQGFMYSIPSIDKSQNNGLVFTALQTRMREIPYKLENNKLVYFENGSGFLRKEYIA
                     NVRFIHRQGFNKTYTFTGGYQYNIISDTLLSLNRDYFVSGHKYESYPVISFEYKDDAR
                     DIRNYPLKGYFLSLEFTKYGFGISDNDPS"
     gene            complement(908..1570)
                     /locus_tag="PK134_08360"
     CDS             complement(908..1570)
                     /locus_tag="PK134_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB38902.1"
                     /translation="MNTKAYIKDFIPPLKSSDTVVRALQWMQAFNLNHLPVVDGVKYK
                     GLITEYDLKKIEDKEKSLSECELEYQNVYIFETQYIYDALEFVTVKGLNIVPVVSKEG
                     NYRGLLTLVDIIDCFAQNSSVKTPGGIIILNVERKNYSMQEIARITESNGALILSSSV
                     NQLPDSEIFEVTLKVDKLDLSRILAGFYRMDYNVVASYQTTDLPTDIEDRYQAFMTYL
                     NV"
     gene            1636..>1861
                     /locus_tag="PK134_08365"
     CDS             1636..>1861
                     /locus_tag="PK134_08365"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRB38903.1"
                     /translation="MTACKLSVNINKIATLRNARGGTIPNVVKAAIDIERFGAEGITV
                     HPRPDERHIRFRDVYELKECVTTEFNIEGYP"
BASE COUNT          616 a          371 c          283 g          591 t
ORIGIN      
        1 cgaaggatcg ttatcgctga tgccaaaacc gtattttgta aactcaagac ttaaaaagta
       61 accttttaaa ggataatttc tgatatcgcg ggcatcatct ttatactcaa aagaaattac
      121 aggataactt tcatacttat gtccactaac aaaataatct ctgttgagcg acagcaatgt
      181 atcggagata atgttgtact gataacctcc ggtaaaagta taagtcttat taaatccctg
      241 ccggtgaata aatcttacgt ttgcaatgta ttcttttctt aaaaatccac ttccgttttc
      301 aaaataaacc aacttgttat tttctaactt atagggaatc tcgcgcattc ttgtttgcaa
      361 agcagtaaac actaacccat tattctgact tttatcaatg cttggaatgc tgtacataaa
      421 tccctgcctt tgcgaataac cataacgaag caacagcctt acattctccc tcctgcctct
      481 gaaattttcc cagtttaaat aaaaaccata attggctttc ttcagatttt tatcgcgcca
      541 ccattcccag aaattacggt caacaacttc gaaaataggt acaggaaaaa tataccaacg
      601 ttcaacaaca tcaattatca cataaacatc gtttccgatt agctgcttgt tgatatttac
      661 aaaattaaaa agcgaaatat tcaataagtt ctctctgctc tttgtgattt tttcatttaa
      721 ttcaccaaca gtcatggtct gcccctgact gaatgccaac tcacgctgaa taacagaata
      781 tcgtgttact ttatttcctg tgacaacaat ctgccttaca taaattgtat tggtatcaac
      841 ctgtaaagaa tcggaagcaa aactgctaaa acaaaatgca aaaaacagat ttattaaaaa
      901 tgctctttta aacattaagg tatgtcataa atgcctgata acggtcttca atatcagtgg
      961 gcaagtctgt agtttggtaa gatgccacca cattatagtc catccgataa aatcctgcaa
     1021 gtattcgcga aagatccagt ttatcaacct ttaaagtaac ctcaaaaatt tcactgtccg
     1081 gaagttgatt tactgatgaa cttaaaatta atgctccgtt cgattcagtt atccgtgcaa
     1141 tttcctgcat agaataattt tttctttcaa cattaagaat aatgattcca ccaggtgttt
     1201 taacagagct gttttgtgca aaacaatcaa taatatcaac taaggtcaga agcccacgat
     1261 aatttccttc tttactgaca acaggaacta tattcagacc ttttactgtt acaaactcca
     1321 atgcatcata aatatattgc gtttcaaaaa tgtaaacatt ctgatattcc aattcacatt
     1381 ctgaaagcga cttttctttg tcttcaattt ttttaagatc gtattcggtt atgaggcctt
     1441 tatattttac cccatcaacc acaggcaggt gatttaaatt aaatgcctgc atccattgca
     1501 atgctctgac aacagtgtcg gaactcttta acggaggaat aaaatcttta atataagctt
     1561 ttgtgttcat gaatattttt tcccaaatcg aatctgccgg caaacatagt acatttgtat
     1621 caattttcag agtatatgac agcatgtaaa cttagtgtga acattaataa aattgcaaca
     1681 ctgcgcaatg cccgaggcgg aactattcca aatgtagtta aggcggcaat agatatagag
     1741 cgttttggtg ccgaaggaat aacagtgcat ccacgtcctg acgaaaggca tatccgtttc
     1801 agagatgttt atgaattgaa agaatgtgtt accacagaat ttaatatcga aggctatcca
     1861 t
//
LOCUS       DAORQT010000306         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13311_length_1856_cov_4.764575, whole genome shotgun sequence.
ACCESSION   DAORQT010000306 DAORQT010000000
VERSION     DAORQT010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13311_length_1856_cov_4.764575"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..554)
                     /locus_tag="PK134_08370"
                     /pseudo
     CDS             complement(174..554)
                     /locus_tag="PK134_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808718.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
     gene            complement(475..>1856)
                     /locus_tag="PK134_08375"
     CDS             complement(475..>1856)
                     /locus_tag="PK134_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38904.1"
                     /translation="TIVYKNMSNIKNQNTMKSIALSAAAIFTLASCTSSQQASNAYSD
                     DVYYSSKANKPAETVQTQQQVQQPQQDVSDQSVSNQGSSTSAENRFDYNDAGTTTQDG
                     NTYVTNNYYSDDDYYDYAYSSRIRRFYRPMWNYGYYDSYYTNSYWYDYNPYDYGVSIY
                     CGYNWWAPSSWWAPSFTYFSWGYTPYNYWWSSPYSYYGYGPYHHHGWNNYSYGYWNGY
                     NNGYWNGYYDGYYNGYGYNPYYFNSYDSYSYNNTYYGPRRSTSGSGSSNAVPPRSIAD
                     SYNRALVKNGRNSMPVSQSFANAMSLPTRDTKDAEVIHPRNNVNTNSPVKDSRNVETA
                     PVRNNDAEVIHPRNNVNTNSPVKDSRNVETAPERNNDAEVIHPRNNVNTNSPVKDSRN
                     VETAPVKNSRADQFSTPRNNNEVKDNSTTPAAPPVRNNSRNEVNPEYNSRTCQTRYER
                     KNDCSDNRY"
BASE COUNT          467 a          344 c          376 g          669 t
ORIGIN      
        1 cgcttgttgg gtggttgata ataagatgcc gtaccatcaa caaatttttt cagatagttt
       61 tttatctcat taaaatgacc tgatgctaac tcaactatct tgcagttgag tggaaaatgt
      121 gttttaatga gttctgctat cttatcgttt accggttcca tagttttaaa attttaataa
      181 gctcgtgcaa aaagcactct ttgtgtactc ggtttacctg taagaataca tttgccgttt
      241 tcttgtttgt tgtttaatgg aatacatcgg atggtagcct tggtttgttc tttaattttt
      301 tcttctgtct cagctgtacc atcccaatgt gcaaatacaa atcctccctt agtgtcgagt
      361 attttaataa actcgtccat actatcagcg acatgcgttg atgtttctct aaagtcaact
      421 gctttcttaa aaatattctg ttggatttgt tcaagaagat tttcaatctt caattcaata
      481 tctgttatct gaacagtcat tttttctttc gtatcgcgtc tggcaagttc tactgttgta
      541 ctcaggatta acttcatttc tgctgttgtt gcgcaccgga ggtgcagcag gagtagttga
      601 gttgtctttt acctcattat tgttgcgtgg cgtagagaac tgatcggctc tgctgttttt
      661 tacaggtgct gtttcaacat tacgagagtc tttcaccgga ctattggtgt tcacattgtt
      721 tcttggatgt attacttcag catcattatt cctttcaggt gctgtttcaa cattgcgaga
      781 gtcttttacc ggactattgg tgtttacatt atttcttggg tgaattactt ctgcatcatt
      841 attccttaca ggagctgttt caacattacg agagtctttt acaggactat tagtgttcac
      901 attatttctt ggatgaatta cttctgcatc ctttgtatca cgtgttggta atgacattgc
      961 attggcaaaa ctctgactta caggcattga gtttcttccg tttttcacca aagccctatt
     1021 gtacgaatcg gctatgcttc ttggcggaac agcattgctg ctgcctgaac cggaagttga
     1081 tcgtcttggt ccgtaatatg tgttgttgta gctatagctg tcgtagctgt taaaatagta
     1141 tggattgtag ccataaccat tatagtaacc atcataataa ccattccagt acccgttgtt
     1201 ataaccattc cagtatccat aactgtaatt gttccagcca tgatgatgat atgggccata
     1261 tccatagtat gaatatggac tgctccacca gtagttgtag ggtgtataac cccaactgaa
     1321 ataagtgaat gaaggagccc accatgatga aggagcccac cagttgtaac cgcaataaat
     1381 gctcacacca tagtcataag gattataatc ataccagtat gagttggtgt aataggaatc
     1441 atagtaacca tagttccaca ttgggcgata aaatctgcgt atgcgtgatg aataggcata
     1501 gtcataataa tcatcatcgc tgtaataatt attagtcaca taggtgttgc catcctgtgt
     1561 tgttgtgcct gcatcgttgt aatcaaaacg gttttctgca gaggtgctgc tgccctgatt
     1621 tgatacagat tgatcgctta catcctgctg aggttgctgc acttgttgct gtgtctgcac
     1681 ggtttcagca ggtttgtttg ctttggatga ataataaacg tcatcggaat aggcatttga
     1741 agcctgttgt gatgaagtgc aggaagcaag tgtaaatatt gctgctgcag atagtgctat
     1801 tgatttcatc gtattttgat ttttaatgtt tgacatgttt ttatacacaa tagttt
//
LOCUS       DAORQT010000307         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13313_length_1856_cov_4.288173, whole genome shotgun sequence.
ACCESSION   DAORQT010000307 DAORQT010000000
VERSION     DAORQT010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13313_length_1856_cov_4.288173"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK134_08380"
     CDS             <1..546
                     /locus_tag="PK134_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38905.1"
                     /translation="PLEFEKPLNVYEYLARITSVEIPVDTGDFRIMDRKIVDVLKHMP
                     EQQKYLRGQIPWIGFRQTYVEYDRKERLAGETGYTYRKMMRFAIDGITSFSTAPLKFA
                     TASGFVVSFISFIWILYALYSRFIIKDYVPGWTSIMIAVLFIGGVQLICIGIIGEYIS
                     RMSANIRNRPLYVVHDSNIQE"
     assembly_gap    610..619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<620..1261)
                     /locus_tag="PK134_08385"
     CDS             complement(<620..1261)
                     /locus_tag="PK134_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRB38906.1"
                     /translation="MIIEIWSDIMCPFCYIGKRHYENALKQFAGKNNIELKWKSYLLD
                     PETPLEFEKPLNVYEYLAERKGISLAQSIKMHEHVVAMAKQAGLDYQLDKLVIANMVK
                     AHRVIQFAKKKGLADLIEENMFTAYFTEGKNLADDDVLVAIGIASGLTKEEVITALTD
                     DLYAYEVDQDIQEARAIGLTGVPFFVFDRKYAISGAQPTEAFLNTLNKSYKEWL"
     gene            1465..>1856
                     /locus_tag="PK134_08390"
     CDS             1465..>1856
                     /locus_tag="PK134_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38907.1"
                     /translation="MKKYILFILLIAVKISIAQQGINNNWLMGYSSFGGLPFGNTKFD
                     FFNGIPIINYDSLEMDFRHTHANISDAQGNLLFYTNGYYIADASNDTMQNGSGINPGA
                     YADQFSDGFQIPQGALIIRQPGSSSIYY"
BASE COUNT          547 a          333 c          342 g          624 t
ORIGIN      
        1 cctcttgaat ttgaaaagcc gcttaatgtc tatgaatatc ttgcccgaat tacctctgtt
       61 gaaattcctg ttgacacagg cgattttcgc attatggacc gtaaaattgt tgacgtgcta
      121 aaacacatgc ctgagcagca gaaatatttg cgcggacaga ttccgtggat tggtttccgg
      181 cagacctatg tagaatatga ccgcaaagag cggcttgcag gtgaaacagg atatacttac
      241 agaaagatga tgcgctttgc tattgatggc attacatctt tctcgacagc gccattaaaa
      301 tttgctactg ccagtggttt tgtagtttct tttatttctt ttatctggat attgtatgcg
      361 ctttactcgc ggtttatcat taaagattat gttccgggat ggacatctat tatgatagct
      421 gttttattta ttggaggtgt gcaacttatt tgtatcggaa taataggaga atacatcagc
      481 cgcatgagtg ccaatatcag aaacagacct ttgtatgtgg tgcatgacag caacatacaa
      541 gagtgattga tagaagaaaa gttttaattg tcacaacctt ctgtgctgca actttctcct
      601 gaggttattn nnnnnnnnna agccattcct tgtaggattt gtttaatgtg ttgagaaatg
      661 cttctgtggg ttgcgcacct gaaatagcat actttctgtc aaagacaaaa aacggaacac
      721 cggttaatcc aatcgctctt gcttcctgaa tatcctgatc tacttcatag gcataaagat
      781 catctgtcaa tgcagtaata acttcttctt ttgttaaacc tgatgcaatg ccaatagcaa
      841 ctaatacatc atcatcagca aggtttttcc cctctgtaaa ataggctgtg aacatatttt
      901 cttcaatcag gtcggcaagt cctttctttt tagcaaactg aataacgcga tgggctttaa
      961 ccatattggc aattactaat ttgtctaatt gataatccaa tcctgcttgt ttagccattg
     1021 ccacaacatg ttcatgcatt tttatggact gtgcaaggct gatgcctttt ctttcggcaa
     1081 gatattcata gacattaagc ggcttttcaa attcaagagg agtttcggga tcaagtaaat
     1141 aacttttcca cttcaactct atattattct taccggcaaa ttgttttaat gcattttcgt
     1201 agtggcgttt gcctatgtag caaaatggac acataatgtc gctccagatt tcaattatca
     1261 tttctatttt agaattttat taagacggat aaacaatgaa ttgtgaaaat agttttttta
     1321 gtattcagcc acaactgata agtttgataa ttctgaaatc tacattctgc attgttttat
     1381 tcttgattac attgtatttt tacaattcaa ttttgtgata cggaactaat aataactcct
     1441 ttttaaactg tacattttta taaaatgaaa aaatacattt tattcatttt actgattgca
     1501 gtaaaaatta gtattgccca acagggaata aataataact ggctgatggg atactccagc
     1561 tttggaggat tgccttttgg caacactaag tttgattttt ttaatggaat accaattatt
     1621 aattacgata gtttggaaat ggactttaga catacccatg ccaatataag tgatgcacaa
     1681 ggtaatttat tgttttacac caacggctat tacatagccg atgccagcaa tgataccatg
     1741 cagaatggaa gcgggataaa tccgggtgcg tatgcagacc agttttcaga cgggtttcaa
     1801 ataccgcaag gtgcactgat tatacgccaa cccggaagca gttcaattta ttatat
//
LOCUS       DAORQT010000308         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13391_length_1849_cov_6.487737, whole genome shotgun sequence.
ACCESSION   DAORQT010000308 DAORQT010000000
VERSION     DAORQT010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_1849_cov_6.487737"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..964)
                     /locus_tag="PK134_08395"
     CDS             complement(140..964)
                     /locus_tag="PK134_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRB38908.1"
                     /translation="MKWFLAIKKQVRPLILWMLLLLQIATIGNAQQIPPQSNPPRLVN
                     DYAGVLNDADENLLEQKLVQFNDSTSTQIAIVLIRSTGEYPVDDYAIALFRDWKIGQK
                     NKDNGVLILAAMEDRRMTIITGYGVEGALPDAICKRIIELTLKPAFKSQQYYNGLDAA
                     VTEIMQRTKGEYVSDGKNAKNNSPAWAPILFVFLIFGVVMLMKVFSVRRYAQLNNLSF
                     WTAWMLLNAASNRRNSSGGGGVWGGGGSSWGGGSSGGGFGGFGGGSTGGGGATGGW"
     gene            complement(942..1382)
                     /locus_tag="PK134_08400"
     CDS             complement(942..1382)
                     /locus_tag="PK134_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRB38909.1"
                     /translation="MANAADYFSDEQQLLLKNAIARAEKHTSGEIRIFIDSKVKSEII
                     DRAAEVFENLKMHETKLHNGVLIYVAIENRQFAIIGDSGIHEYVSQVFWDKVKEEMIP
                     FFKRKDYTGGLLHAIEETGQKLKKYFPVAKNDSDELSNEVVFSD"
     gene            complement(1385..>1849)
                     /locus_tag="PK134_08405"
     CDS             complement(1385..>1849)
                     /locus_tag="PK134_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRB38910.1"
                     /translation="VENQYQQRMDLIPNLVNTVKGAAEFEKGTLTAVVEARASATQVK
                     VDPDKLTPENIQKFQEAQGQLSTALGRLLMVTENYPVLKANQNFLELQATLEGTERRI
                     AVARKDFNEAAQQFNTYIRKFPQTLLAGMFGFSPKGYFQSDKGANKAPEVKF"
BASE COUNT          523 a          414 c          322 g          590 t
ORIGIN      
        1 actaaagaat gttaaagagc cacaattcct acggaatttt acagcgcatt ttgagccact
       61 gtaatttttg tgattagcaa ttgaacattg attaatgcgg aaagcagcat tatcgaaaag
      121 ttgaagttgt aaatagcatt taccagccac cggtggcgcc accccctccg gtgctgccgc
      181 caccgaagcc tccaaatcct cctccgctac tgccaccgcc ccaactgctg ccaccgcctc
      241 cccaaactcc accacctcct gatgagttac gtctgttact cgctgcattc aaaagcatcc
      301 aggctgtcca gaaacttaaa ttgttgagct gagcatagcg cctgacagaa aaaactttca
      361 tcagcattac tacaccgaaa attaaaaaca caaaaagtat gggtgcccat gcaggtgaat
      421 tgttctttgc atttttgcca tcagaaacat attcgccttt tgttcgctgc ataatttctg
      481 ttactgcagc atcaagtccg ttgtaatact gttgtgattt gaatgcaggc ttcagcgtaa
      541 gctcaataat tcttttacaa atagcgtcag gtaatgctcc ttcaacaccg taacctgtaa
      601 tgatggtcat gcgcctgtct tccatagcag ctaaaatcag cacaccatta tctttattct
      661 tctgaccaat tttccaatct ctgaaaagag ctatagcata atcatccaca ggatattcac
      721 cggtactgcg aatcagtaca atagcaattt gtgtagaagt gctgtcgtta aattgtacaa
      781 gtttttgctc taaaagattt tcatcagcat cattcaaaac tcctgcatag tcgttgacta
      841 agcgtggtgg attactttga ggtggaattt gttgtgcatt accaattgtt gcaatctgaa
      901 gcaacaataa catccataat ataagaggac gtacctgttt tttaatcgct aaaaaccact
      961 tcattggaaa gttcgtctga atcattctta gcaacaggaa aatacttttt taatttctga
     1021 cctgtttctt cgatagcatg caacaagcca ccggtataat cttttctctt aaagaatgga
     1081 atcatttcct ctttaacctt atcccaaaaa acctgactta catattcgtg aataccacta
     1141 tcaccaataa tggcaaactg tctgttttca atggctacat aaataagcac gccattgtga
     1201 agtttggttt catgcatctt caggttttca aaaacttcgg ctgcacggtc aataatttca
     1261 ctctttacct tgctgtcaat aaaaattctg atttctccgg atgtatgttt ttctgctctt
     1321 gctattgcat ttttcaacaa caattgttgt tcatcagaaa aataatcggc agcgttggcc
     1381 ataattaaaa cttaacctca ggggctttat tagcgccttt atcagattga aaataacctt
     1441 ttggtgaaaa cccaaacatg ccggcaagca atgtttgagg aaacttacgt atgtaggtgt
     1501 taaactgttg tgcagcctcg ttaaaatctt tacgtgccac agcaattctt ctttctgttc
     1561 cttcaagtgt tgcctgcaat tcaagaaagt tctgattggc tttcagtacc ggataatttt
     1621 cagtcaccat aagtaagcga cctaatgctg tgcttaactg cccttgtgct tcctgaaact
     1681 tctgaatatt ttcaggtgta agtttgtcag gatcaacttt tacttgtgtt gccgatgcac
     1741 gtgcttcaac aactgctgtt aaagtgcctt tttcaaattc tgcagcacct ttaactgtat
     1801 ttactaagtt tggaataagg tccattcgtt gttggtattg gttttcaac
//
LOCUS       DAORQT010000309         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13597_length_1829_cov_7.129087, whole genome shotgun sequence.
ACCESSION   DAORQT010000309 DAORQT010000000
VERSION     DAORQT010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13597_length_1829_cov_7.129087"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(512..1507)
                     /locus_tag="PK134_08410"
     CDS             complement(512..1507)
                     /locus_tag="PK134_08410"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRB38911.1"
                     /translation="MEKIRAAITAVHAWVPDYVLTNKELETMVETNDEWIQTRTGIKE
                     RRILKDAGEGTSKIGVESVNGLLKKRGIQAEEIDLLICATTTPDLVFPATANIICDKV
                     GAKNAFGYDIQAACSGFLFALTTASKFVESGQYKKVVVVGADKMSAIIDYSDRATCII
                     FGDGGGAVLLEPDTNGYGIYDAVLKSDGSGRHFLHQKAGGSVKPPTHATIDAKEHFVY
                     QEGQTVFKFAVKGMADVSAEIMERNNLSADDIAWLVPHQANKRIIDATANRMGIGMEK
                     VMLNIQKYGNTTNGTLPLCLWEWEKQLHKGDNLILAAFGGGFTWGAVYLKWAYNS"
     gene            complement(1517..>1829)
                     /locus_tag="PK134_08415"
     CDS             complement(1517..>1829)
                     /locus_tag="PK134_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HRB38912.1"
                     /translation="FNNKADVIVCDGFTGNVMLKEAESLYTLLKKRGIKDAYFDRFDY
                     ENYGGTPVLGVNGNVIIAHGISKSKAIKNMILLAKEVTEAHLAEKIATFFQNNVVNQT
                     L"
BASE COUNT          544 a          390 c          296 g          599 t
ORIGIN      
        1 gtgcagacct gcctgctcta tcatttcaag aaaatttccc tttctgattc ctatcagcaa
       61 cgaaaccttt atattttcat ttaactgttg ttgttgcgca agagtgatta ccaacttctc
      121 tataccacca aaatttgcag accaaataaa atgtaacact ctcatttttt aagatattta
      181 gttaatcata aacaagtctc ttctcctgtt taataattaa aaaaactaaa attaataacc
      241 gaatcagata aaaggcaaaa gtaaactttc actcgatttt gttcaacaga aattaaaaaa
      301 gacaagtagc gacttttatt aatcgaatgg gtattgcaga cgctcgttta ccatagattg
      361 cttttatatt ttcaccacca ttcattacaa ccttattttt gttgtaattt tcttcaaagc
      421 acctctaaaa atttaaactt ttttaagagt gaaataaaga gtttttcgga attgaataat
      481 gcggttggta ttttctaaga atatgaattc cttagctgtt ataagcccat ttaagataaa
      541 ctgctcccca agtaaagcct ccgccaaaag cggcaagaat caggttatca cccttgtgca
      601 attgtttttc ccattcccat aaacagagtg gtaatgtgcc attggttgta tttccatact
      661 tctgaatgtt gagcatcact ttttccatgc caatgcccat tcggttggca gtagcatcaa
      721 taattctttt gtttgcctga tgtggaacaa gccatgcaat atcatctgca ctcaggttgt
      781 tacgttccat aatttctgca cttacatcag ccataccttt aacagcaaac ttaaaaaccg
      841 tttgcccctc ctgatagaca aaatgttctt tggcatcaat agttgcatga gttggaggtt
      901 tcacactacc acctgctttc tgatgcagaa aatgtcgtcc actgccatcg ctttttaaaa
      961 cagcatcgta aatgccgtaa ccgttggtat ccggttctaa taatacagca ccaccaccat
     1021 caccaaaaat aatgcaggtg gcacggtcgc tgtagtcaat gattgccgac attttatcgg
     1081 cacccacaac aacaactttt ttatactgtc cgctttcaac aaattttgaa gcagtagtca
     1141 atgcaaataa aaatcctgaa caggctgcct gaatatcgta gccaaaagca tttttagcac
     1201 ctactttatc acaaataata tttgctgtgg ccggaaaaac caaatcagga gtagtagtgg
     1261 cacaaatcag caagtcaatt tcttctgcct gtatgccacg cttttttaat agcccgttta
     1321 ccgattcaac accaattttg gaagtacctt caccggcatc ttttaatatt ctgcgttcct
     1381 taattcctgt tcttgtttga atccactcat cgttggtttc aaccatggtc tccagttctt
     1441 tgtttgtaag aacatagtcc ggcacccagg catgaacagc ggtgatggca gctctgattt
     1501 tttccatcaa aaaaaattat agtgtttgat taactacgtt gttttgaaaa aaagttgcga
     1561 ttttttctgc aagatgggct tcggtaactt ctttggcaag caatatcatg tttttgatgg
     1621 ccttcgattt tgaaatgcca tgtgcaataa ttacatttcc gtttacacca agcaccggtg
     1681 tgccaccata attttcataa tcaaagcggt cgaagtatgc atctttaata ccgcgttttt
     1741 taagtagcgt ataaagtgat tcggcttctt taagcattac attacctgtg aagccatcgc
     1801 atacaatgac atcagctttg ttgttgaac
//
LOCUS       DAORQT010000310         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13759_length_1814_cov_7.080159, whole genome shotgun sequence.
ACCESSION   DAORQT010000310 DAORQT010000000
VERSION     DAORQT010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13759_length_1814_cov_7.080159"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK134_08420"
     CDS             <1..599
                     /locus_tag="PK134_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38913.1"
                     /translation="YNTSVGCIGNYISPVHVYSHASTGGCSVTGGFVYRGTAYPALYG
                     YYFFADFCNGTIYALDPNNGNAVTVAGTFPGRAFSTFGEDDNGELYIADLVNQKVYQV
                     TDTTTSISEYGNDVNNVDMSFNQNEQSLQMNLTSKSNNQATLYLYDLKGTLITAWKLS
                     LKSGNNTFKLPINLTSGIYLSNINSVNASLNIKIPVIK"
     gene            complement(605..>836)
                     /locus_tag="PK134_08425"
     CDS             complement(605..>836)
                     /locus_tag="PK134_08425"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRB38914.1"
                     /translation="AHCGWPSFDDEIKGSVKRIPDADGMRTEIVCSNCDGHLGHVFLG
                     EGFTAKNTRHCVNTSSLKFIPAGTPLPEVIKK"
     assembly_gap    837..879
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<880..1085)
                     /locus_tag="PK134_08430"
     CDS             complement(<880..1085)
                     /locus_tag="PK134_08430"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRB38915.1"
                     /translation="MKIRIFNIALVTLMGMWQLSIAQNSRKMEYNKLTPQEEKVLLFK
                     VTDRPFTGDYYLKKDVGTYICRRC"
     gene            1211..>1814
                     /locus_tag="PK134_08435"
     CDS             1211..>1814
                     /locus_tag="PK134_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB38916.1"
                     /translation="MNFDLIIIGSGPGGYVAAIRASQLGLKTAIIEKENLGGICLNWG
                     CIPTKALLKSASVFEYLNHASDYGITVEGGKADFNAVIKRSRGVADGMSKGVQFLMKK
                     NKIEVINGFGKVKPGKSVEVTATDGSKKTYTAAHIILATGARSRELPNLKQDGKNIIG
                     YREAMSLPEQPKSMVVVGSGAIGVEFAYFYNAMGTKVTVVE"
BASE COUNT          565 a          335 c          338 g          533 t
ORIGIN      
        1 cttacaatac aagtgtaggg tgcataggaa attatatttc acctgtccac gtatatagcc
       61 atgcatctac cggtggatgt tctgttacag gcggatttgt atatcgtggc actgcctacc
      121 ctgctcttta tggatattat ttctttgccg atttctgtaa tggaacaata tatgctttag
      181 accctaacaa cggcaatgcc gttaccgttg caggaacatt tcccggacgt gcattcagca
      241 cctttggtga agatgataat ggtgaattgt atattgcaga cttagtgaat cagaaggtgt
      301 atcaggttac agacacaact acttctattt ctgaatatgg taatgatgta aataatgttg
      361 acatgtcttt caatcaaaat gagcaatcac ttcaaatgaa tttaacttca aaaagcaaca
      421 atcaggcaac cctttatctt tatgatttaa aaggcacttt aattacagct tggaagttga
      481 gtttaaaatc aggtaacaac acattcaaac tacctattaa tcttacctca ggaatttatt
      541 taagcaacat taatagtgtc aacgcttcgc tcaacattaa gataccggta ataaaataag
      601 cttactactt tttgattact tcaggtagag gtgttcctgc aggtatgaat tttaatgagc
      661 tggtgtttac acagtgtctg gtgtttttag ctgtaaatcc ttcgcctaaa aaaacatgac
      721 ctaaatgccc gtcacaatta ctgcaaacaa tttctgtgcg cataccatct gcatcaggaa
      781 ttcgttttac agagcctttt atttcatcat caaaactggg ccaaccacaa tgcgccnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tacacctgcg acaaatataa
      901 gtgcctacat cttttttaag ataataatca ccggtaaaag gcctgtccgt tactttaaac
      961 aacaacactt tctcctcctg aggtgtcaat ttattgtatt ccatttttct gctgttttgc
     1021 gcaatgctta gctgccacat tcccattaag gtaactaagg caatattaaa tattctgatt
     1081 ttcattgaca aaatttattt gatacctcca cgctatcaat atgtacaaac acatttacag
     1141 aaacaaagtt aatacctgct atcagattca ttaaaaagct atattttagc agcaaattaa
     1201 agcatcaatt atgaattttg atttaatcat tataggaagt ggccccggag ggtatgtagc
     1261 agctatcaga gcatcgcagt taggacttaa aacagcaatt attgaaaaag aaaacctagg
     1321 tggtatttgc ctgaactggg gttgtattcc tactaaggca ttgctcaaaa gtgcaagtgt
     1381 ttttgaatac ctgaaccatg cttctgacta tggtattact gttgaaggtg gcaaagccga
     1441 ctttaatgct gttatcaaaa gaagtcgtgg cgttgcagat ggcatgagca agggcgttca
     1501 gtttctgatg aaaaaaaata aaatagaagt catcaatggt tttggaaaag taaagcccgg
     1561 aaaaagtgta gaagtaacag caaccgatgg cagcaaaaaa acatatacag cagcgcatat
     1621 tattcttgca acaggagcac gttcgcgcga gttgcctaac ctgaagcaag atggcaaaaa
     1681 cattattggc tatcgcgaag caatgagcct gcccgaacaa ccaaagtcaa tggtggtagt
     1741 gggttcaggt gctattggag tagagtttgc ctacttctat aatgctatgg gcacaaaagt
     1801 tactgtggta gaat
//
LOCUS       DAORQT010000311         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_13849_length_1805_cov_6.777714, whole genome shotgun sequence.
ACCESSION   DAORQT010000311 DAORQT010000000
VERSION     DAORQT010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13849_length_1805_cov_6.777714"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PK134_08440"
     CDS             complement(<1..1350)
                     /locus_tag="PK134_08440"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRB38917.1"
                     /translation="MQLLKEVLYKAGTEEVIGNTNVAVYGICINSRVIKPGEMYVALR
                     GTNVDGHAFILEAIEKGATSVLCEVMPDLIQAGINYIKVKNTYLALSEVCANFYHHPS
                     SSLKLVGITGTNGKTTTATLLYKLFRQLNIRCGLISTVENRVNDTVIPATHTTPDPIK
                     LNQLLSMMVLEECEYCFMEVSSHAVVQNRIGALEFAGGVFTNITHDHLDYHKTFEEYL
                     KAKKKFFDGLPESAFALTNADDKNGMVMVQNTHAAKKTYGIADFRCRIIENNFSGLLL
                     QIDNTEVLCKLIGAFNAYNLLAIYATAVSLGLDKQRVLTALSMLESVNGRFDYVVSGS
                     GITGIVDYAHTPDALNNVLSTINEIRTRNEKVITVVGCGGNRDTAKRPVMAHIACEMS
                     DKVILTSDNPRNEDPQAIIEQMRAGVPAHLSSKVMAITDRNEAIRVACNLAAAGDIIL
                     "
     gene            complement(1350..>1805)
                     /locus_tag="PK134_08445"
     CDS             complement(1350..>1805)
                     /locus_tag="PK134_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRB38918.1"
                     /translation="VVVYNPSTAGYYGGEVALPVFKDIADKVYATNLDMHKGLDVQIA
                     ELPKVKAGLTKATIAACNNVGVDVKTVGTASKWSSVDKREAVAALKPVSIKDNTVPNV
                     EGMGLRDAIYMLETNGLQVKVVGRGAVVKQSLQAGTRIYRGQVITIELS"
BASE COUNT          520 a          388 c          316 g          581 t
ORIGIN      
        1 gaggataata tctcctgcag cagcaaggtt gcatgccaca cgaatggctt cattgcgatc
       61 ggttattgcc atcactttag atgataaatg tgcgggcaca ccggcacgca tctgttcaat
      121 gattgcttgg gggtcttcgt ttcgcggatt gtcagaagta agaataacct tatcactcat
      181 ctcgcaggca atgtgagcca taacagggcg ttttgctgta tcacgatttc caccgcaacc
      241 aactactgta attactttct catttcttgt tcttatttcg ttgatggtag aaagtacatt
      301 attcaatgca tcgggtgtat gtgcataatc aacaatgcca gtaataccgg aaccggaaac
      361 aacataatcg aatctgccat tgacagattc gagcatactc aatgcagtaa gcactcgctg
      421 cttatccaat cctaatgaaa cagcagttgc ataaattgct aaaagattat aggcattgaa
      481 tgcaccaata agtttacaca acacttctgt attatcaatc tgcagcaaca gaccactaaa
      541 attattttca atgattctgc atctgaaatc ggcaatacca taagtttttt tggcagcatg
      601 tgtattctga accatcacca ttccgttttt gtcatcggca ttagtcaatg caaatgcact
      661 ttcaggaaga ccgtcaaaaa atttcttttt tgctttcaga tattcttcaa aagttttgtg
      721 atagtcaaga tgatcgtgtg tgatattggt aaaaacacca ccggcaaatt ctaatgctcc
      781 tattctgttt tgtaccacag catgtgagct gacttccata aagcagtact cacattcttc
      841 caacaccatc atgcttaata gctgattgag ttttattgga tcgggtgttg tatgtgttgc
      901 aggaataaca gtatcgttaa ctctgttttc tacagtagaa attaatccgc aacgaatatt
      961 cagttggcgg aataatttat agagcaatgt ggctgtagtt gttttaccat tagttccggt
     1021 aattccaact aattttagag aagaagaagg atggtgataa aagtttgcac acacttcaga
     1081 aagtgccaga taggtatttt ttacttttat ataattgata cctgcctgaa tgagatctgg
     1141 cataacttcg cataaaactg aagtagcgcc tttttctatg gcttctaaaa taaaagcatg
     1201 cccatcaaca ttggtaccgc gaagtgcaac atacatttct ccgggtttaa ttacgcgtga
     1261 gttaatgcat attccatata cagctacatt ggtatttcca atgacctcct ctgttccagc
     1321 tttgtataaa acttctttca gtaactgcat caacttaact ctattgttat tacctgacct
     1381 ctataaattc ttgttcccgc ctgtaatgat tgcttcacca cagcaccacg tccaactact
     1441 ttaacctgca atccatttgt ttcgagcata taaattgcat cacgcaaacc cattccttca
     1501 acatttggaa cagtattatc tttaatgcta acaggcttca atgctgcaac agcttctctc
     1561 ttatcaactg atgaccattt tgatgcagtg ccaacagttt ttacatcaac accaacattg
     1621 ttacatgcag cgattgttgc tttagtcaga cctgctttta cctttggtaa ttctgctatt
     1681 tgcacatcaa gtcctttgtg catatcgaga ttggttgcat aaactttatc tgcaatatct
     1741 ttgaatacgg gtaatgctac ctcgccacca tagtaacctg ccgttgaagg gttatacact
     1801 actac
//
LOCUS       DAORQT010000312         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_14176_length_1775_cov_8.002907, whole genome shotgun sequence.
ACCESSION   DAORQT010000312 DAORQT010000000
VERSION     DAORQT010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_1775_cov_8.002907"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>1775)
                     /gene="purL"
                     /locus_tag="PK134_08450"
     CDS             complement(157..>1775)
                     /gene="purL"
                     /locus_tag="PK134_08450"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRB38919.1"
                     /translation="VYIVGSATGKDGIHGATFASGDLHEDSHEDLPSVQVGDPFQEKL
                     LLEATLEVIATGAVVGMQDMGAAGIICSTSEMSAKGNHGMIIHLDKVPTRQKNMEPWE
                     ILLSESQERMLIVAEKGKEHLINAVFEKWDLNCTCIGEVVKGEQLKFYMNETLVADVN
                     AESLVLGGGAPVYKRDYKEPAYFKQNKTFNIDNVKVPIDLTAVAKQLASHPNICSRRW
                     VYTQYDSMVGTINMSTNKPSDAAIVNIKNSKKALAVSVDCNARYVFADPEQGCAIAVA
                     EAARNIVCTGGEPSAVTNCLNFGNPYNPEVYWQFVHSIKGMSKACEKFQTPVTGGNVS
                     FYNQSKIGNDDVPVYPTPTIGMIGIISDKNKAATLAFSNAGDQIFLIGTSKNCIASSQ
                     YLVSTHGINNSPAPEFNLDEEYDVQQLIKQLIKTGYVQSVHDVSDGGLFITLLESAMI
                     KGLGFEINSDEDIRKDAFLFGESQSRVVVSVSEEQMDEFISLVADSGVDFTNLGVVTH
                     GEIIVDEVKFGNISEYSKLYQEKLESEMAY"
BASE COUNT          530 a          359 c          303 g          583 t
ORIGIN      
        1 ttactaaagg tgtcattgga atgtgggtag caaacatttc agttgctgaa tgagttatta
       61 aaattccaaa actgttttct ttaataaagt caacttgttg gttaaaattc gtatttcgaa
      121 aatattcagg aatgtacata attgaaaaga atacttttag taagccatct cactttcaag
      181 tttttcctga tagagttttg aatattcgct gatgttgcca aatttaactt cgtcaacaat
      241 tatttcacca tgtgtcacaa cacccaggtt ggtgaagtcc acgcctgaat cagctactaa
      301 gctgataaat tcgtccattt gttcttcaga aacactaact acaacacgac tctgtgattc
      361 tccaaaaaga aatgcatctt ttcttatatc ctcatcagag ttaatttcaa aacccagacc
      421 ctttatcatt gcagattcaa gtaaagtaat aaataatcca ccatcagaaa catcatgtac
      481 agattgtaca tatcctgttt tgattaactg cttgatgagt tgttgaacat catattcttc
      541 atcgagatta aattcaggcg cagggctgtt gttgatacca tgtgtgctga caagatattg
      601 tgatgaagca atacagtttt tagaagtgcc aatcagaaat atctgatcac ctgcatttga
      661 aaatgccaat gttgcagctt tgtttttatc actgataatt ccaatcatgc caatagttgg
      721 agtagggtaa acggggacat cgtcatttcc gattttcgac tgattataaa aactaacatt
      781 accacctgtc accggagttt gaaatttttc gcatgctttt gacatacctt ttatgctatg
      841 cacaaactgc cagtaaactt caggattata tgggttgcca aagttaaggc aattggtaac
      901 agcagaaggc tctcctcctg tacagacaat attgcgtgct gcttctgcaa ctgcaatagc
      961 gcagccttgt tcaggatctg caaaaacata tcttgcatta caatcaacag aaactgcaag
     1021 tgctttttta gaatttttaa tatttacaat tgcagcatca gatggtttgt ttgtactcat
     1081 attaattgtg cccaccatac tgtcgtattg tgtgtaaacc catcgtctgc tacaaatatt
     1141 cggatgtgat gctaattgtt tggcaacggc agtaagatca ataggaactt ttacattatc
     1201 aatgttaaat gttttgtttt gtttgaagta tgcaggctct ttataatctc ttttatagac
     1261 aggagcaccg ccacctaata caagtgattc agcattaaca tctgcaacca acgtctcatt
     1321 catatagaat tttaattgct cgcctttaac tacttcgcca atgcatgtgc agtttaaatc
     1381 ccatttttca aaaaccgcat taattaaatg ttctttacct ttttctgcaa caatgagcat
     1441 tctttcctgc gactcggaaa gtaaaatttc ccaaggttcc atgttttttt ggcgcgtagg
     1501 aactttgtca aggtgtataa tcatgccatg atttcctttg gcagacattt ctgaggtaga
     1561 acaaataatt cctgcagcac ccatatcctg cattcctaca actgcaccgg ttgcaatgac
     1621 ttcaagtgtt gcctctaata aaagtttttc ctgaaatgga tcgcctacct gcacagatgg
     1681 taaatcctca tgcgaatctt catgcaggtc gcccgatgca aaagtggcac catgaatacc
     1741 gtcttttcct gttgctgagc ctacaatata aacac
//
LOCUS       DAORQT010000313         1766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_14299_length_1766_cov_4.821742, whole genome shotgun sequence.
ACCESSION   DAORQT010000313 DAORQT010000000
VERSION     DAORQT010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14299_length_1766_cov_4.821742"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PK134_08455"
     CDS             complement(<1..239)
                     /locus_tag="PK134_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38920.1"
                     /translation="MNVLSEFIQQESGQKIIIAHPESEEELNQYYQLRHEILRAPWNQ
                     PPGSEKVNDDNEAIHLSLLIDDLMIGVCRLHFNNA"
     gene            complement(244..>1766)
                     /locus_tag="PK134_08460"
     CDS             complement(244..>1766)
                     /locus_tag="PK134_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38921.1"
                     /translation="TSLCDANGYSGFNGAWSVMNSGTNGNRANMFYVSCSEANRPVNT
                     CAGGCATGGDATLHVGTSPCATCLICPTGDCGAIYDTLASGGFDPTTDKRAVSPPINT
                     VGKSNMSISFKYINVGDLRSPASSTDKALLEYSVDNGLTWTALINIPRSSTVGGCALY
                     KRWTAFGPLNLPAACNDLPNLLIGFRWINNNNSIGATINTNGSFAVDEVNISGKSAES
                     FTTEYFHINPTVIYNNVVNPPLDHVSRMEYWYMTKEIGDKRKITLYWDGNSGGVTSLP
                     DLRVARFNGASWDDLGNTGTTGTAAAGTITSDSTKDYGPVTLASVIALPGNPLPVELL
                     SFNGNRHNDEIKLNWLTSSELNNHYFTLKRSGNNLQYTELGKVNGAGTTNLPQRYDYI
                     DSKPLRGINYYKLFQTDFDGTTEEKATLAIRFDAYDGDVLVLPDFNHEQVLLQFSENN
                     KEPVNIMLMDAAGRLMLQTVCSESNTCRLLTSHLSRGVYVLRMQTADGVIVKRFFY"
BASE COUNT          521 a          362 c          329 g          554 t
ORIGIN      
        1 tcagcattgt taaaatgtaa tcggcaaaca cctatcatta aatcatcaat cagcagacta
       61 agatgaatcg cttcattatc atcattaact ttttcactgc ctggaggttg gttccatggt
      121 gctcttaaaa tttcatgtct gagctgataa tactgattca gttcttcttc gctttcagga
      181 tgagctatga ttattttttg tccactttct tgttgaataa attcagaaag aacattcaca
      241 actttagtag aaaaatcttt ttactataac accatctgct gtttgcatgc gcaacacata
      301 aacacctctt gaaaggtgag atgttaataa acggcaagtg ttgctctctg aacacacagt
      361 ttgtaacata agtcgcccgg ctgcatccat tagcattatg tttacaggtt ctttattgtt
      421 ttcggaaaac tgaagcaata cctgttcatg attaaaatcc ggcagaacta aaacatcgcc
      481 atcataagca tcaaaacgaa ttgccaatgt ggccttttcc tctgtggtgc cgtcaaaatc
      541 ggtttgaaaa agtttgtaat aattgattcc gcgcaatggc ttgctgtcta tataatcgta
      601 acgctgaggt aaattggttg ttccagcacc atttactttg ccaagctctg tgtattgtaa
      661 attatttccc gatcttttca aggtgaaata atgattattt aattcagaag atgtcagcca
      721 gttgagttta atttcatcat tatggcgatt accattaaaa gatagtagtt caacaggtag
      781 aggattgccg ggtaatgcta ttacagaagc taaagttact ggtccataat ctttagttga
      841 atcggaagta attgtaccgg cagcagcagt tcctgtggtg cctgtatttc ctaaatcatc
      901 ccatgatgca ccattaaaac gtgccactct aaggtcgggc aggctggtga caccaccact
      961 gttaccatcc cagtaaagtg taatttttct cttatcacca atctctttag tcatatacca
     1021 atattccatc cggcttacgt ggtcgagtgg tggatttaca acattgttat agattacggt
     1081 tgggtttatg tggaaatatt ctgtggtaaa cgactctgcg cttttgcctg atatattcac
     1141 ttcatcaaca gcaaatgaac cgtttgtgtt aatagttgca cctatgctat tgttattatt
     1201 tatccatcga aagcctataa gtaagtttgg taagtcatta caagcagcgg gtagattaag
     1261 cgggccaaag gcagtccagc gtttatataa agcacatcca cctacagtac ttgatcgtgg
     1321 aatgtttatt aatgctgtcc atgtcaatcc attatctacg ctatattcaa gtaaagcctt
     1381 atctgtactt gaagcagggc ttcttaaatc gccaacatta atgtatttaa aactaataga
     1441 catatttgat tttcccacag tattaattgg tggcgaaaca gctcttttgt cagttgtagg
     1501 gtcgaagcca ccacttgcta aagtatcata aatggcacca caatcaccag tcggacaaat
     1561 aaggcatgtt gcacaaggcg aagttccaac atgcaaagta gcatcgccac cggtagcaca
     1621 accacctgca catgtattaa cgggtctatt ggcttcggaa caactaacat agaacatatt
     1681 tgctctgttg ccattagttc cggaattcat aacagaccat gcgccattaa aaccactgta
     1741 gccattggca tcacatagcg atgtac
//
LOCUS       DAORQT010000314         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_14505_length_1750_cov_7.077876, whole genome shotgun sequence.
ACCESSION   DAORQT010000314 DAORQT010000000
VERSION     DAORQT010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14505_length_1750_cov_7.077876"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PK134_08465"
     CDS             complement(<1..1144)
                     /locus_tag="PK134_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HRB38922.1"
                     /translation="MIISDSIKTTSYKVVLPFYIYAAFAFLLSAILLLTSSSAFTDHY
                     FQPHILAITHLMALGWGTMIILGASHQLVPVLIEGKLYSNALAYASFILAGIGIPLLV
                     YGFYVFNMHNPAKWGGRFILLSIIAYLINLGVSMSKSKKENIHALFVFTSALWLFLTG
                     ALGLALVYNFTYNMFPHDSLHYLPLHVHAGVIGWFLMLIIGVGSRLIPMFLISKYTNT
                     KLLKWIFVLINGALISYILIFYFVKITEVILLPWLMLFTGIALFIFYCYSAFKHRIRK
                     QVDEPMKVSLLAVMLLMIPLLLLLISIVSAVIFSQEDIALSLSYGFLIFFGWITAIIL
                     GMTFKTLPFIVWNKVYHKRSGIGKTPNPKDLFSNTVFKLMSVAYIAG"
     gene            complement(1156..1701)
                     /locus_tag="PK134_08470"
     CDS             complement(1156..1701)
                     /locus_tag="PK134_08470"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRB38923.1"
                     /translation="MPEVFTTLKKEQIVILDVRPVLAAGNDPLNLILEKVNSLEAGKC
                     LKIINTFEPTPLIIMLEKKGFVTYTDTISDELIETYFYKKSDNKNEINPVVNASGGWD
                     EIMQQYNNKLQTIDVRAMEMPLPMLTILDALEKLPSNHALYVYHKRIPVFLLPELAEK
                     KFDYRIKEISDGEVHLLIFKS"
BASE COUNT          619 a          296 c          294 g          541 t
ORIGIN      
        1 gtcctgcaat ataggccaca ctcattaatt taaatacagt attactaaat agatcttttg
       61 ggttaggtgt ttttccaatg cccgaacgtt tatgataaac tttgttccac acaataaatg
      121 gaagtgtttt aaatgtcatt cccagaataa ttgccgttat ccatccaaaa aatatcagaa
      181 agccataact taaagataag gcaatgtctt cctgcgaaaa aattacagct gaaacaatag
      241 aaatcaatag caacaacaat ggtatcataa ggagcattac agccaatagc gaaactttca
      301 ttggttcgtc aacctgtttt ctgatacgat gtttaaaagc tgaataacaa tagaaaataa
      361 aaagtgcaat tcctgtaaaa agcatcagcc aaggtaatag aattacttca gtgattttca
      421 caaagtaaaa tatcagaata taggatatca atgcaccgtt tatcaataca aaaatccatt
      481 tgaggagttt tgtgttggta tatttagaaa ttaaaaacat gggtatcagc cttgagccta
      541 cacctattat cagcatcaga aaccaaccaa tcacacctgc atgcacatga agcggtaaat
      601 aatgtaaaga atcatgcgga aacatgttgt atgtaaagtt atatactaat gcaagaccca
      661 gagcacccgt aagaaataac cacaatgccg atgtaaaaac aaataaagca tgaatatttt
      721 cttttttgct tttcgacata cttacaccaa gattaatcaa gtatgcaatg attgataata
      781 atataaacct gccaccccat ttggcaggat tatgcatgtt gaatacataa aatccataaa
      841 ctaataacgg aataccaatt cctgcaagaa taaatgaagc atatgctaat gcattgctat
      901 aaagttttcc ttcaatcaat acgggaacaa gttgatggct tgcgcctaat attatcatag
      961 taccccaacc cagtgccatc agatgtgtga tggctaaaat gtgaggctga aaataatgat
     1021 ctgtaaaggc agaagaagat gttaagagta aaattgcaga cagtaagaat gcaaaagcag
     1081 cataaatata aaatggcaac accactttat aggatgttgt ttttatggaa tcagaaatga
     1141 tcatacttta atgcattacg acttgaaaat aagcaggtgc acttcaccat cagatatttc
     1201 ttttattcgg taatcgaatt tcttttcagc taattcgggt agcaaaaata caggaatacg
     1261 tttatgataa acatataatg catgattgga tggtagtttt tctaaagcat ctaaaattgt
     1321 aagcatcgga agtggcatct ccatagcacg cacatcaata gtttgaagtt tattattgta
     1381 ttgctgcatg atttcgtccc atccacccga agcattaact acaggattta tttcgttttt
     1441 gttgtctgac tttttgtaga aatatgtttc aatgagttca tcactaatgg tgtctgtata
     1501 tgttacaaaa cctttttttt ctaacatgat gatgagtgga gtaggctcaa aagtgtttat
     1561 tatttttaaa cattttccgg cttcaagact gttgactttt tcaagaataa ggtttagtgg
     1621 atcattgcca gcagctagca caggtctgac atcaagtata actatctgtt ctttttttaa
     1681 cgtggtaaaa acctcaggca tgataatcga gtgcttcttt attaactggc ataattattt
     1741 tttttacaaa
//
LOCUS       DAORQT010000315         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_14520_length_1749_cov_5.500590, whole genome shotgun sequence.
ACCESSION   DAORQT010000315 DAORQT010000000
VERSION     DAORQT010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_1749_cov_5.500590"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..521
                     /locus_tag="PK134_08475"
     CDS             213..521
                     /locus_tag="PK134_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRB38924.1"
                     /translation="MDNKNENQLNIELSEEIADGIYSNLAIITHSNSEFVVDFIKVMP
                     GVPKAKVKSRIVLTPQHAKRLMTALAENVAKYEQAHGTIKQTDGIAIPMNFGGPTAQA
                     "
     gene            complement(560..1516)
                     /locus_tag="PK134_08480"
     CDS             complement(560..1516)
                     /locus_tag="PK134_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB38925.1"
                     /translation="MCRNVYHVGPADRSIGSQASNAPNNYGMRFDVLQEVTIDSVYIY
                     PVTAGTVTINLVDATGANILNTKTITVPNTFNIYPVYLGFSVTGGTSYRLIVASGSVN
                     LYYNTSGAIYPYAVAGSPLSITGYYNPNPNTGAAYYYLYNWVITSGCKSGYIPVTGVV
                     QGPPMPTITPSGSLLTSSTAANYQWLLNGNIIPGANSQTYQTTQPGSYTVQVTDATTG
                     CIVTSLPFMIVSISDPNAIAAGVSIYPNPSTDKFYLNFNEAVLGNASVKLFNGVGQLI
                     YVTEVNNVTGKTVELEKDLSKGIYFMQLTTEKGTFQTKIIRE"
     gene            complement(1476..>1749)
                     /locus_tag="PK134_08485"
     CDS             complement(1476..>1749)
                     /locus_tag="PK134_08485"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38926.1"
                     /translation="AIVTSGGCPSATSNMLSVTVIPVTTPTTVNDTICAGGTANLQSS
                     GTGIVNWFTSQTGDTLVNTGNTYNVSLAATTTFYVQKCISCRSCRS"
BASE COUNT          534 a          338 c          328 g          549 t
ORIGIN      
        1 ctcgaacccg cgaccccatg cgtgacaggc atgtattcta accaactgaa ctaccggacc
       61 ctcttttaga gggcgcaaag atagattttt tattctttct acaaataatc tttacttttt
      121 tattgctatt tctgttcaag tcagcccttc gcctgtgaat tttaaataag acggcttgtg
      181 aattaaccgt atctttgaag aaaattcaga caatggacaa taaaaacgag aatcagttaa
      241 atattgaact gagtgaggaa attgcagatg gtatttattc caatctggcc atcataaccc
      301 attcaaactc cgagtttgtg gttgatttta ttaaagttat gcctggtgtt cctaaggcaa
      361 aagttaaatc aagaattgtt ttaacgcctc aacatgctaa aaggctgatg acagctttag
      421 ccgaaaatgt tgcaaaatat gagcaggcac atggcacaat taaacaaact gatggcatag
      481 ccataccgat gaatttcggt gggccaacag cacaagctta aaaaagaaac cgcctcaagg
      541 gcggtttctt ttttggtttt tattcgcgaa taatttttgt ctgaaatgtc cctttttctg
      601 tggtcagttg cataaagtag attccttttg ataaatcttt ctctaattct actgttttac
      661 ccgttacatt atttacttca gttacataaa tcaactgtcc tactccatta aataatttca
      721 ccgaagcatt tcctaaaaca gcttcattaa aattcaagta aaatttatct gttgatgggt
      781 ttggataaat gcttactcct gcagcaattg catttggatc gcttatagaa acaatcataa
      841 aaggtaatga agttactatg catcctgttg tagcatcagt cacctgaaca gtataagaac
      901 ccggttgtgt tgtctgataa gtctgactgt ttgctcctgg aataatattt ccattcaata
      961 accactgata atttgctgct gttgatgaag ttaaaagtga accacttgga gtaattgttg
     1021 gcattggcgg accctgcacc actcctgtta cagggatata accactctta catccactgg
     1081 tgataaccca attgtagaga taataatatg ctgcaccggt atttggatta gggttataat
     1141 atcctgtaat tgataatggg ctacctgcaa ctgcataagg ataaattgca ccagatgtgt
     1201 tgtaataaag atttacgctt cctgaggcaa ctatcaatct atatgatgtt ccaccggtaa
     1261 cactgaatcc aagatatacc ggataaatat taaaagtatt aggaactgta atagttttgg
     1321 tgtttagaat atttgctccg gtagcatcta caagattaat ggtaactgtt cctgctgtaa
     1381 ccgggtaaat ataaactgag tcaattgtta cttcctgcaa aacatcaaaa cgcattccat
     1441 agttatttgg agcattgctt gcctggctac cgatactacg atcggcagga cctacatgat
     1501 atacatttct gcacataaaa tgtagttgtt gcggctaatg aaacattata tgtattacct
     1561 gtattaacca acgtatcacc tgtttgcgaa gtaaaccagt ttacaatgcc tgtaccgctt
     1621 gattgcaggt tagctgttcc accggcacaa attgtatcgt tcacagtagt tggtgttgtt
     1681 acaggaatca cagtaacaga aagcatatta gaagttgccg aagggcagcc accgctggta
     1741 actatggcc
//
LOCUS       DAORQT010000316         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_14568_length_1745_cov_3.051479, whole genome shotgun sequence.
ACCESSION   DAORQT010000316 DAORQT010000000
VERSION     DAORQT010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14568_length_1745_cov_3.051479"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK134_08490"
     CDS             complement(<1..336)
                     /locus_tag="PK134_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRB38927.1"
                     /translation="MISKQTLSFLRALAVNNNREWFAANHHKYEGAKEDFNNYLKQTL
                     TELAKYDVLLKSLEPKDCVFRIYRDIRFSPDKTPYKTNLGAAINAGGKRVETAGAYLH
                     IQLGNKSFFA"
     gene            complement(365..>1745)
                     /locus_tag="PK134_08495"
     CDS             complement(365..>1745)
                     /locus_tag="PK134_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38928.1"
                     /translation="YVGGHWNNSGTFTAGQSDFTFNGANPQNIDGTNNTTFMAMSVSK
                     PALIAVRLNRDVVVTDTLNLTQGRLQLNSVAPTAIRSLSLMSNTGSTTALTRNTGVLI
                     AESNPPNYGNFRWKIGTPAAYPTTFTFPFANAAGTYIPVDYKVNNGDADVTLTTYTTI
                     PANTPIPTGVSDIYMNTWFAPPFTDNSPYMVDRFWQINDAGTVSSRDLTLNWTPTENA
                     SAGSSPYRAQVYSSSTNWGYPFIIGQTNPTATSVSIPAATFTNTWAVVRELQPLPVEL
                     LSFEAKPYKDQEVVCNWTTATELNNDFFTVERSSDGRNFEKVGTVKGAGNSTQQHDYK
                     FIDTKPYRGVSFYRLKQTDFNGKSESFEKVAVNLSLPATPISVYPVPANEYVVFSVDR
                     NQNLENAVLYVYDFTGRALIQKKVAELKGTGTNIFILPVNTLINGLYHFDLKVEGASL
                     GNGKFIVEK"
BASE COUNT          485 a          343 c          347 g          570 t
ORIGIN      
        1 ggcaaaaaac gacttgttgc caagctgaat gtgcagatag gctcctgcag tttctactct
       61 tttacctccg gcattgatag ctgcacccag atttgtttta taaggtgttt tatcgggcga
      121 aaaacgtatg tcgcgataaa ttctgaaaac acaatctttg ggttcaaggc ttttgagcaa
      181 aacatcgtac ttcgccaatt ccgttagcgt ttgtttgagg taattattaa aatcttcttt
      241 agcaccctcg tacttgtgat gatttgcagc aaaccactca cggttgttat taacggcaag
      301 tgcccgtaag aatgacagtg tttgtttact tatcatactt acgggcactt tgtcttttaa
      361 aaaattattt ctctacaata aatttaccgt ttcctaatga agctccctca acttttaaat
      421 cgaaatgata caaaccattg attaaagtat ttacaggtag gataaagata ttggttcctg
      481 tacctttaag ttctgctact tttttctgaa tcaatgcacg tcctgtaaag tcataaacat
      541 acaatactgc attttcgaga ttctgatttc tgtcaacact gaatactaca tattcgttag
      601 caggaacagg ataaacactt attggtgttg caggcaaact caagtttaca gctactttct
      661 caaacgactc agacttaccg ttaaagtctg tctgtttcag tctgtagaaa gaaacccctc
      721 tgtaaggttt agtatcaata aatttatagt cgtgttgttg cgttgaatta ccggcacctt
      781 ttaccgtacc cactttttcg aagttacggc catcgctgct gcgttcaaca gtaaagaaat
      841 cgttgtttaa ttctgtagct gttgtccagt tacaaactac ttcctgatct ttatatggtt
      901 tagcttcaaa cgaaagtaat tctacaggca acggttgcaa ttctcttaca actgcccatg
      961 tgtttgtgaa ggtagctgca ggaatagaaa ctgatgttgc cgttggattt gtctgaccga
     1021 tgataaaagg ataaccccaa ttagtacttg aactatacac ctgtgcacga taaggagacg
     1081 agcctgctga tgcattctct gttggtgtcc agtttaatgt aaggtcgcgg ctgctaactg
     1141 tacctgcatc atttatctgc cagaaacggt caaccatata ggggctgtta tctgtaaatg
     1201 gcggtgcaaa ccaggtgttc atataaatat ctgacacgcc tgttggtatt ggtgtatttg
     1261 caggtattgt tgtataagtt gttaaggtaa catcagcatc gccattattt actttatagt
     1321 caacaggaat gtatgttcct gcagcgttag caaacgggaa tgtaaatgta gttggataag
     1381 ccgcaggtgt tccaattttc caacggaagt tgccatagtt tggtggattt gattctgcta
     1441 tcaatacacc agtatttcta gttaatgccg tagttgaacc cgtattactc attaaactca
     1501 aagagcggat tgcagttggg gcaacactat taagttgtaa tctgccttga gttagattta
     1561 gtgtatctgt aaccactaca tctctattta atctaaccgc tataagagca ggcttgctta
     1621 ccgacattgc catgaaagtt gtgttattgg taccatcaat attttgtggg ttggcaccat
     1681 taaaagtaaa atcgctctgc cctgctgtaa atgtaccgga gttgttccag tgaccaccta
     1741 catag
//
LOCUS       DAORQT010000317         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_14650_length_1739_cov_4.923990, whole genome shotgun sequence.
ACCESSION   DAORQT010000317 DAORQT010000000
VERSION     DAORQT010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14650_length_1739_cov_4.923990"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..978
                     /locus_tag="PK134_08500"
     CDS             58..978
                     /locus_tag="PK134_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HRB38929.1"
                     /translation="MENNPLLCNPDSGICEMPTNKATATQDGKIKSEKRPIKIVYYTD
                     PICSSCWGIEPQLRKLKLEYSSNIEVEYRMGGLLPDWSYNSGGISKPSDVAHHWDEVS
                     VYYDMPIDGDVWLEDPLQSSYPPSIAFKAAQMQDQDKAILFLREIREMVFLKKKNIAK
                     WEHLESAAKSVGLDVGKFREDYNGKAKELFNEDLALGKQLGVRGFPTMFFTDTSGKTE
                     TVYGSKPYAAFENAIIKLQPDVQKFNYDKSVAAVFANYHSLTAKEYSELTGTARQQSE
                     QVLNDLVANGTLEKLTTKNGAIWKLKNAGQ"
     gene            complement(1126..>1739)
                     /gene="rho"
                     /locus_tag="PK134_08505"
     CDS             complement(1126..>1739)
                     /gene="rho"
                     /locus_tag="PK134_08505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRB38930.1"
                     /translation="VTDMARSVKAEVISSTFDEPADKHVKIANLVLEKAKRLVECGHD
                     VVIVLDSITRLARAYNTTAPASGKILSGGVDSNALHKPKRFFGAARKIEKGGSLTIIA
                     TALIDTGSRMDEVIFEEFKGTGNMELQLDRKLSNKRVYPAIDLVASSTRREDLLLDRK
                     MLERMWILRNHLADMNPMEAMEFLLDRLRGTKSNEEFLISMNS"
BASE COUNT          571 a          318 c          334 g          516 t
ORIGIN      
        1 tatttaacag tatttatatt cggcaatacc aactgcttag cacaaacaaa aaagaaaatg
       61 gaaaacaatc ctttattatg caatcctgat agtggtattt gcgaaatgcc aacaaacaag
      121 gcaactgcaa cacaagacgg caaaataaaa tcggaaaaaa ggccaataaa aattgtttac
      181 tatacagatc cgatttgctc ttcttgttgg ggaattgagc cacaattacg caaattaaaa
      241 ttggaataca gcagcaatat tgaagttgaa tacagaatgg gtgggttact gcccgattgg
      301 agctacaata gcgggggcat aagtaagcca tctgatgttg cccatcactg ggatgaggta
      361 agtgtttatt atgatatgcc tattgatggt gatgtttggt tagaagatcc tttgcaatcc
      421 tcatatcctc cttccatagc atttaaagct gctcagatgc aagatcagga taaggctatt
      481 ctgtttttaa gagagataag agaaatggtt tttctgaaga aaaagaacat tgcaaagtgg
      541 gagcatttag agtcggcagc taagtcggtt ggattagatg taggtaagtt tagggaggat
      601 tacaatggaa aagctaaaga gctttttaat gaagacttag cattaggcaa gcagttaggt
      661 gtaagaggtt ttccgactat gttttttaca gatacatcag gaaagacaga aactgtatat
      721 ggttcaaaac cttatgctgc ttttgaaaat gcaattatta aacttcagcc tgatgtacaa
      781 aaattcaact atgataaaag tgtggcagca gtttttgcaa actatcattc attaactgct
      841 aaagaatatt cggaattaac aggaacggca agacagcaaa gcgaacaagt ccttaatgat
      901 ttggttgcaa atggaacatt agaaaaactg acaactaaaa acggagcaat ttggaaattg
      961 aaaaatgcag ggcaatagta agtgggatat taattgtcta aactatggtg caacactcat
     1021 gtaacagtaa atagagatac atttttacat tccacattct tcaaatctct aattctctaa
     1081 ttccacaacc ttccaaacct tctaactttt taactttcca aacttctagc tgttcataga
     1141 gataagaaac tcttcgtttg atttagttcc tcttaatcgg tcaagtaaaa actccattgc
     1201 ttccattggg ttcatgtcgg ctaagtgatt tcttaaaatc cacatacgct ccaacatttt
     1261 gcggtcaagt aataaatctt cacgtcttgt acttgaagca actaagtcaa ttgccggata
     1321 aacacgtttg ttgcttaact tgcggtcaag ctgcaactcc atgttacctg tgcctttaaa
     1381 ttcttcaaag ataacttcgt ccatgcgcga accggtgtca attaaagcag ttgcaatgat
     1441 ggttaatgaa ccaccttttt ctattttacg tgctgcacca aaaaatcttt ttggtttgtg
     1501 cagtgcatta gagtcaacac caccggataa tatttttcct gatgcaggag cagtggtgtt
     1561 ataggctcgt gccaatcggg ttatagaatc caatacaatc actacatcat gtccgcactc
     1621 aaccaaacgt tttgcttttt ccaaaacaag gttcgcaatt ttgacatgct tgtctgcagg
     1681 ctcatcaaag gtcgaagaaa taacttcggc ttttacactg cgtgccatat ctgtaacct
//
LOCUS       DAORQT010000318         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_15053_length_1710_cov_3.126284, whole genome shotgun sequence.
ACCESSION   DAORQT010000318 DAORQT010000000
VERSION     DAORQT010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15053_length_1710_cov_3.126284"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PK134_08510"
     CDS             complement(<1..494)
                     /locus_tag="PK134_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38931.1"
                     /translation="MKKLIIVLSVAATAAIIGCSKDDLTSPQALQNKNTTTATNDVSA
                     TDERLINSGTVLVPAPNQGYVKVFLFGAAKLSADKPETFVDINKRDVSQRAFDASFKA
                     MYVKFNERWIYVPGIIPNYGIEGKLTYRVSSSQASDPDATNQILAWHRFTLTIRNLNN
                     RPYTG"
     gene            complement(781..1446)
                     /locus_tag="PK134_08515"
     CDS             complement(781..1446)
                     /locus_tag="PK134_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB38932.1"
                     /translation="MALSNLKILIADDHKLFAEGLTYLLKQELPIESIDTVYNGKSAI
                     GKVLNTDYDIIIMDVNMPLLNGIETCAAIKRFRTQTKIVFVSMKADMLTVAQALKAGA
                     DAYILKGNDHNDFITAFKQINNNEIFLSEQIKHYFQDDNPNQIRKNAIACVDYSNRLI
                     YNIKKEIIKLVCDGLTSDKIAEVLSISTRTVDTHRNNILNKLKLNNIASLVRFALENK
                     LVD"
     gene            complement(1456..>1710)
                     /locus_tag="PK134_08520"
     CDS             complement(1456..>1710)
                     /locus_tag="PK134_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38933.1"
                     /translation="NNTLKYAHASIISIIVLGDRDKFYFIYEDNGLGFDLKNSGRGKG
                     LNNILQRVKELGTKTEFIAQPGGGMRCEFKIDYEQSMQIA"
BASE COUNT          481 a          294 c          304 g          631 t
ORIGIN      
        1 cctgtatatg gtctgttgtt taagttgcga atggtgagtg taaaccgatg ccatgcaaga
       61 atttggtttg tagcgtcagg gtcgctggct tgtgatgatg aaactctata agttagttta
      121 ccttctatgc catagtttgg aattattcct ggaacatata tccaacgctc attgaatttt
      181 acatacatag ctttgaagct ggcatcgaat gctcgttgcg atacatctct tttgttgatg
      241 tcaacaaatg tttcgggttt gtcggcagag agttttgccg caccaaaaag aaacactttg
      301 acataacctt ggttgggcgc aggcaccaat acagtgccgg aattgatgag gcgttcatct
      361 gttgcactaa catcatttgt tgctgttgtg gtgtttttgt tttgcagagc ctgtgggctg
      421 gtgagatcgt ccttgctgca accgataatt gccgcggttg ctgctacact taatacaata
      481 atcagctttt tcatcttgac taatttttaa ctgttagaca ttttgttggt taactatttt
      541 tgttggagaa caactgtccc cttaattaat tcaacttttg ttgttttata aatcagtgta
      601 aaaattatat catgttgttt attttgatga tttgtatttg aacttctaac tccctttgta
      661 tttgttgttg tgaaattgat agtgtaaaag taagcccgca aactactgaa tgcaatacgt
      721 aaaacttcgt aatttttaat tattagaatt aagtgctgat tttatttctt gcagaagaaa
      781 ttaatccacc agcttattct ctaaggcaaa gcgaaccaat gaggcaatgt tgtttagttt
      841 tagcttattg agtatattgt ttctgtgtgt atcaaccgtt ctggttgaga tagaaagtac
      901 ttcagcaatt ttatcactcg ttaatccatc acaaactaat ttaataattt ctttttttat
      961 attatatata agtctgttgg aataatctac acaggcaatt gcatttttac ggatttgatt
     1021 aggattatca tcctgaaagt aatgtttaat ttgttcactt agaaaaatct cattgttgtt
     1081 aatctgttta aatgcagtta taaaatcatt atgatcgtta ccttttaata tgtatgcatc
     1141 ggcacctgct tttaatgcct gtgcaactgt tagcatatcg gctttcatgc tcacaaaaac
     1201 tattttggtt tgtgttctga atctttttat cgctgcacag gtttctatgc cgtttaataa
     1261 aggcatatta acatccataa taataatatc ataatctgta ttcaacactt ttccaatggc
     1321 tgatttcccg ttataaaccg tgtcaatact ttctattggt aactcctgct ttaaaagata
     1381 ggtgaggcct tcggcaaaaa gcttatggtc atcagcaatc aaaattttta agttacttag
     1441 tgccatatta aataatcagg ctatttgcat tgattgttca tagtcaattt tgaattcaca
     1501 gcgcatgcca ccaccgggtt gagcaataaa ctctgtcttg gtgcctaatt cttttacgcg
     1561 ctgcaaaata ttatttaagc ccttgccacg acctgagttt tttaaatcaa agcccaaacc
     1621 attgtcttca tagataaaat aaaacttatc cctatcgcct aaaacaatga tgcttataat
     1681 agaagcatgg gcatatttta gagtgttatt
//
LOCUS       DAORQT010000319         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_15091_length_1706_cov_4.724409, whole genome shotgun sequence.
ACCESSION   DAORQT010000319 DAORQT010000000
VERSION     DAORQT010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15091_length_1706_cov_4.724409"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..786
                     /gene="truB"
                     /locus_tag="PK134_08525"
                     /pseudo
     CDS             76..786
                     /gene="truB"
                     /locus_tag="PK134_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            complement(892..1602)
                     /gene="pyrH"
                     /locus_tag="PK134_08530"
     CDS             complement(892..1602)
                     /gene="pyrH"
                     /locus_tag="PK134_08530"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRB38934.1"
                     /translation="MKYKRILLKLSGEALMGEKNFGIDNNRLTQYAEEIKTIVDKGVQ
                     VAIVIGGGNIFRGLQAAEGGMDRVQGDYMGMLATVINSMALQGALEHKGINTRLQSAI
                     TMDAICEPFIRRRAVRHLEKNRVVIFGAGTGNPYFTTDTAASLRAIEVEADVILKGTR
                     VDGIYTSDPEKNSDAVRFEHLTFDDVYEKDLKVMDMTAFTLCHENKLPIIVFDMNTPG
                     NLLRLVEGNHVGTLVDVE"
BASE COUNT          498 a          353 c          305 g          550 t
ORIGIN      
        1 gtacagaata attattggtg ctgctatttt gctgttgcac tttgcaggca ttgaacttaa
       61 aatgctttaa tctgcatgcc tgattattta caaggtgaag tgctgttggt agataaacct
      121 ttagagtgga catcttttga tgtagtgaat aaattaagag gcaccttaca gcatctgaaa
      181 tttgaagacg gcagcagacc ttataaaaaa ataaaagttg gacatgccgg aactttagac
      241 ccactagcaa cgggtttgct tattgtttgc accggtaagg ccacaaaaag tattgactca
      301 ctaatgggtc ttgaaaaaga atatacaggc acttttactc ttggacaaac tacaccatcg
      361 tatgatgcag agacaacagt tgacaggaca tttgatatat cgcacatcac tacggagcta
      421 atccatcagg cagcacaaac ttttgttgga gaaacagagc aactgccacc tgcacattct
      481 gccgtaatta ttcagggtga acgtgcttat gaaaaagcaa gacgtggcga agaagtaaaa
      541 gttacaccat gaaaaattaa tatacaaagt tttgagatta cagcaatagc tatgcctgtt
      601 gtttcatttc gtgttgtatg cagtaaagga acctacatac gttctttagc caacgatttt
      661 gggaaagcat taaactccgg tgcatatctg agctcactca tccgcacatg cattggtgat
      721 tttaaactta gcaatgcaca cagtgttgaa cattatgttg aagcaataag aagtttgcag
      781 aagtagtttt tttagggggt ggctagtttt gaaatgaatc attctccttt tagtatttca
      841 ttatcaaaaa aatcttttcg aaattctatt caccaaaaat cttcttgcaa tttattccac
      901 atcaaccagc gtgcctacat gattgccttc tacaagtcgc aacagattgc ccggtgtatt
      961 catgtcaaag actatgatag gaagtttgtt ttcatgacac agtgtaaatg cagtcatatc
     1021 cataaccttt aaatcttttt cgtaaacatc atcaaaggtc agatgttcaa agcgcactgc
     1081 atcgctattc ttctcaggat cggatgtgta gatgccatca acccttgttc ctttaagaat
     1141 tacatcggct tctacttcta tggcacgtaa tgatgctgct gtatctgttg taaaataggg
     1201 attaccagtt cctgcaccaa aaatcacaac tctgtttttt tcaaggtggc gcactgccct
     1261 tcttcttata aaaggctcac agatggcatc catagtaatg gcagattgca aacgcgtgtt
     1321 tatgccttta tgttccaatg caccttgcaa agccatacta ttaattactg ttgccagcat
     1381 acccatgtaa tcgccctgca ccctgtccat gccgccttct gctgcctgca accctctgaa
     1441 aatattacct ccgccaataa ctattgcaac ctgaacacct ttgtcaacaa ttgtttttat
     1501 ctcctctgca tattgggtaa gcctattgtt gtctatccca aaatttttct ctcccattaa
     1561 tgcttcaccg ctaagtttca gtaatattct tttatacttc atatcagatt tgtgttttta
     1621 tctttttagt tttttctttt aatcttttga aaaataatcg gcactttgat aggctcctgt
     1681 aagctgcttt attatctgca tcagta
//
LOCUS       DAORQT010000320         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_15130_length_1703_cov_7.491505, whole genome shotgun sequence.
ACCESSION   DAORQT010000320 DAORQT010000000
VERSION     DAORQT010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15130_length_1703_cov_7.491505"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>227
                     /locus_tag="PK134_08535"
     CDS             <1..>227
                     /locus_tag="PK134_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38935.1"
                     /translation="IYNYAGDLNGQPIYVDANANGTPLYTAGMLGYRCDTGYTVRTNG
                     NDPFFHFLKGPVEEFDFNANDRIDFTVTPQSG"
     assembly_gap    228..258
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <259..>1703
                     /locus_tag="PK134_08540"
     CDS             <259..>1703
                     /locus_tag="PK134_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38936.1"
                     /translation="MTVVAGRLANGPEHLKFGLNINGTGMIEGVQMDTLPKECDGDPL
                     NGIPNAVPYTQTWDITDFNSLTPVTFTIGCFGGLNNVAIVYQVIIHGTVTPAQTIPEI
                     SSYSHPGGYMYCDDESVTLSVVATGTPSPSYQWQDQYGNAILGATQSTLTLPQPLPQH
                     SYYRVKVYNTFGETYSATTHIYGYVNDIYAGTDQLLCAGDQATLNGQGSGFLNWNTLG
                     DGSFLDPYSLNTIYYPGPGDSLAGNVQLEFYGYVYNNYGYEVCWRQDNLNLTIGIPPT
                     ANAGNDQTISQSNTILTANDPSPGNGLWTVISGTATFNDATLYNTSVSNLSNGTNLLR
                     WTITMGTCSSYDEMTVTVATTPVSAIISGDTTICNGGQTQLKIDFTGNGPFDYSYTNG
                     TTVFGPFTAVTNPEIIWVNPASTTNYQLVSVSSGGTSGTVSGSANIQILTALPSSNVT
                     LPFNGMPTHICNGTTANLSIASVAGATNYIW"
BASE COUNT          515 a          336 c          313 g          508 t
ORIGIN      
        1 atctacaact atgctggcga cttaaatggt caaccaattt atgttgacgc aaatgccaat
       61 ggaacgcctc tctacactgc cggtatgtta ggataccgat gcgacacggg atatacggtt
      121 aggactaatg gcaacgatcc attttttcac ttcttaaaag gtccggtgga agaatttgat
      181 ttcaatgcta atgaccgtat tgattttaca gttacacccc aatcaggnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnga tgacagttgt agccggaaga cttgcaaatg gccctgagca
      301 tctaaaattt ggtttaaaca ttaacggtac aggaatgata gaaggcgttc aaatggacac
      361 cttacctaaa gaatgtgatg gcgatccttt aaatggaatt cctaatgctg ttccttatac
      421 acaaacatgg gatattaccg attttaattc tcttacacct gttactttca caataggatg
      481 cttcggaggg ctaaataatg tagctatagt ttatcaggta attattcatg gcactgtaac
      541 accagcacaa actattcctg aaatttcaag ctactcccat cccggtggct atatgtattg
      601 tgatgatgaa tctgtaacac tatccgtggt tgccacaggt accccttctc cttcttatca
      661 atggcaggac caatatggta atgcaatttt gggagcaaca cagtcaacac ttactttacc
      721 tcaacctttg cctcaacaca gttattatcg cgtaaaagtt tataacactt ttggagaaac
      781 ttattctgcc actacgcata tttatggtta tgttaatgat atctacgctg ggactgatca
      841 gctattatgt gcaggagatc aggctacact aaacggtcaa ggatctggct ttttaaattg
      901 gaatacatta ggtgatggaa gttttcttga tccttattca ttaaacacaa tttattatcc
      961 cggacctggt gattcacttg caggtaatgt acaacttgaa ttttatggat atgtttataa
     1021 taactatgga tatgaagtat gctggaggca agacaattta aatcttacta taggcatacc
     1081 accaacagca aatgcaggaa atgatcagac aatttcgcaa tcaaatacta tcctgactgc
     1141 aaatgaccca tcacccggaa atggcttatg gacagtgata tcaggaactg caacttttaa
     1201 tgatgctacc ctatataata cgagcgtcag taatttaagc aatggcacta atttattacg
     1261 atggaccatt acaatgggta cttgttccag ttatgatgaa atgactgtta ctgtagcaac
     1321 aacaccggtt tctgctatta tttcggggga cacaactatt tgtaatggag gacaaactca
     1381 acttaaaata gattttacag gaaatggacc ttttgactac agttatacaa atggtactac
     1441 tgtttttgga cctttcacag ccgttactaa ccctgaaata atatgggtta atcctgcttc
     1501 taccaccaac tatcaattgg tatcagttag ttcaggaggt acgagtggca cagtttcagg
     1561 atcagccaat attcaaattt taactgcact tccatcatct aacgtgacat taccctttaa
     1621 tggaatgcca actcatatat gtaatggaac aactgcaaac ttatccatcg cttctgttgc
     1681 aggtgctacc aactatatat ggg
//
LOCUS       DAORQT010000321         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_15189_length_1699_cov_5.560219, whole genome shotgun sequence.
ACCESSION   DAORQT010000321 DAORQT010000000
VERSION     DAORQT010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15189_length_1699_cov_5.560219"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>218)
                     /locus_tag="PK134_08545"
     CDS             complement(<1..>218)
                     /locus_tag="PK134_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRB38937.1"
                     /translation="GAATVILESLESAQKRGATILAEVTGYGFSSNGDHISNPDFNGQ
                     ERSLNMAMKLAGINASNIDYVNAHATST"
     assembly_gap    219..228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<229..916)
                     /locus_tag="PK134_08550"
     CDS             complement(<229..916)
                     /locus_tag="PK134_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRB38938.1"
                     /translation="MQRVVITGIGIYSCIGKNLAEVTESLRQGKSGIIFDQERKDLGF
                     RSALTGFVETPDLKDFLHRRDRLGMHQPAMYAFMSTREALAMAKLDTDYMEKNETGII
                     FGNDSTAGSVVETIDKARKKKDNTLIGSGDIFQNMNCTVNMNLATIYKLKGINFTLSA
                     ACASGSHAIGMGYLLIRQGLQERVICGGAQEINPPAMTSFDGLGTFSSRENEPQKASR
                     PFDKSRDGLIP"
     gene            complement(920..1651)
                     /gene="fabG"
                     /locus_tag="PK134_08555"
     CDS             complement(920..1651)
                     /gene="fabG"
                     /locus_tag="PK134_08555"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRB38939.1"
                     /translation="MNCALVTGASRGLGKAIALQLAADHKLHILINYASNKQAAEETL
                     TTIKNAGGSGELLPFNVQDNETVNAALDHWHKENENKFISVLVNNAGITRDGLFMWMP
                     EKDWDDVLNISLKGFYNVTRNVVNPMLRKRAGRIVNIVSLSGLKGNAGQTNYAAAKGA
                     IVAATKSLAFEVAKRKITVNAVAPGFIKSDMTEGLNEKELSKLVPMERFGTPEEVAHV
                     VSFLASDKASYITGEIININGGIYS"
BASE COUNT          455 a          362 c          310 g          562 t
ORIGIN      
        1 tgttgaggta gcatgggcat ttacgtagtc aatatttgat gcattaattc ctgctaactt
       61 cattgccatg tttaatgagc gttcttgccc attaaaatca ggatttgaaa tatgatcgcc
      121 attggatgaa aatccatagc ctgttacttc agcaagtatg gtagcacctc ttttctgtgc
      181 cgattcaagc gactctaaaa ttacagtggc agcaccacnn nnnnnnnnaa ggaattagtc
      241 cgtcacggct tttatcaaat ggacgcgatg ctttttgtgg ttcattctca cgactgctga
      301 atgtgccaag cccatcgaag ctggtcatag caggtggatt tatttcttgt gcaccaccgc
      361 agataacacg ctcctgtaat ccttgacgta tcaacaggta acccatacca atggcatgtg
      421 atccacttgc gcaagctgca ctaagtgtaa aatttattcc tttaagctta taaatagtgg
      481 caaggttcat gtttaccgtg cagttcatgt tttgaaaaat atcgccactg cctattaaag
      541 tattgtcttt cttttttctt gccttgtcaa tagtttctac tacagaacct gctgtgctgt
      601 cattgccaaa aatgattcct gtttcatttt tttccatata gtctgtatcg agctttgcca
      661 tagcaagtgc ttcgcgtgtt gacatgaatg catacattgc cggttggtgc atacccaaac
      721 ggtcacgtct gtgaagaaaa tctttaaggt caggtgtttc tacaaaacct gtcaatgcag
      781 aacggaatcc taaatctttt ctttcctgat caaagataat tccactcttt ccctgacgta
      841 acgactctgt aacttctgca aggttcttgc cgatacatga ataaataccg atacctgtga
      901 tgactactct ttgcataaat caggaataaa tacctccgtt aatgttgatg atttcgcctg
      961 taatataaga ggctttgtct gatgccagaa agctgacaac atgtgctact tcttcaggtg
     1021 tgccaaaacg ttccatcgga actaatttac ttaattcttt ttcattaagt ccctctgtca
     1081 tatcactctt gataaatcca ggcgcaacag cgttgacagt gatttttctt ttagcaacct
     1141 caaaagcaag tgattttgtt gcagcaacaa tagcaccttt ggcagccgca taatttgttt
     1201 gtccggcatt tcctttcaat cctgagagtg aaacaatatt gacaatacgg cctgcgcgtt
     1261 tgcgaagcat tgggttcaca acgtttcggg taacattata aaatcctttg agtgaaatat
     1321 ttaaaacatc atcccaatct ttttctggca tccacataaa aagaccatca cgggtgatgc
     1381 ctgcattgtt taccaataca ctgataaatt tattttcatt ctctttatgc caatggtcaa
     1441 gagcagcatt tactgtttca ttatcctgaa cattaaatgg cagcaactca ccactacctc
     1501 cggcattttt aattgttgtc aatgtttctt cagcagcttg tttgtttgat gcatagttga
     1561 tgagaatgtg cagcttatga tctgctgcaa gctgaagtgc aatggctttg ccaagtcctc
     1621 ttgatgctcc ggttactaaa gcgcaattca tgatttaaag ttcgttttct tttaaaatat
     1681 caataacttt tctgatgtt
//
LOCUS       DAORQT010000322         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_15270_length_1693_cov_7.878510, whole genome shotgun sequence.
ACCESSION   DAORQT010000322 DAORQT010000000
VERSION     DAORQT010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15270_length_1693_cov_7.878510"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PK134_08560"
     CDS             complement(<1..878)
                     /locus_tag="PK134_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRB38940.1"
                     /translation="MKSAIKGFRSAVLKSAIVFAVCMLLVYLLAWLIPFNVNIPYAVT
                     VTDSRGEVLHAFLSKDEKWRMKANLNEINPQLVKAIIQKEDRWFYFHYGVNPVAVGRA
                     VVNNLIYQRKTSGASTITMQVSRLLSPAPRTLFNKMFEAFRAVQLENRYSKKEILEMY
                     LNLIPYGGNIEGVKAASVLYLDKLPADLSLAECVTLCIIPNRPNSLRPGYDNDLIRRE
                     RNKWLTRFERNKVFKKEIINDALNEPVEMKRQAAPALITQLAYRLKEQYPTIENIHST
                     LQAPIQHKVQSLVYNY"
     gene            complement(875..>1693)
                     /locus_tag="PK134_08565"
     CDS             complement(875..>1693)
                     /locus_tag="PK134_08565"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRB38941.1"
                     /translation="SQRSLSGNFSSYIRDQALALNCLLETDHDNAQIPTMARTLSQLI
                     RGEKWLSTQERAFALLALGKFAKRSTSANVRATVFADGKQIGTFNGNDLVINSNLAGK
                     KIEIRTEGKGQLYYFLEVSGIKESLRLKDEDSKLNVRRSYFNRFGQQINNNFKQGDLI
                     VVQLALSSPTGIEVENVVLTDLIPSGFEIENPRIVDDREMTWIKSKSQPDYFDIRDDR
                     INLYTQAGKTTKYFYFTVRAVNTGSFVVGPVSADAMYDGEFHSYSGGGRVKVVK"
BASE COUNT          490 a          367 c          277 g          559 t
ORIGIN      
        1 ctgtaattat aaacaagcga ctgtacttta tgctgtattg gtgcttgtaa ggtagaatga
       61 atattctcaa tggttggata ctgttccttt agccgatatg ccaactgtgt aattaatgca
      121 ggtgcagcct gtcgcttcat ttcaacgggt tcatttaatg catcgttgat gatttctttt
      181 ttaaaaactt tattcctttc aaatcttgtc agccatttat ttctttcccg tcttatcaga
      241 tcattatcgt aacccgggcg cagagagttt ggtctgttag gaataatgca taacgttaca
      301 cactctgcaa gagataagtc tgcaggcaat ttgtcgagat acaaaactga tgcagctttt
      361 accccttcaa tatttccacc atagggaatc agattgagat acatctctaa aatttctttc
      421 tttgaatacc tgttttccaa ttgtactgca cggaatgctt caaacatttt gttaaacaag
      481 gtgcgtggtg caggactaag caatcttgac acctgcatgg tgatggttga tgcaccggaa
      541 gtttttcgct gataaattaa attattcact actgccctgc caacggctac gggatttacg
      601 ccataatgaa aataaaacca acggtcttcc ttttgaatta tagcttttac caattgtgga
      661 tttatttcat tcaagtttgc tttcatccgc cacttttcat ctttagacaa aaaagcatgc
      721 agcacttcgc cacgactgtc agtaactgta acagcataag gaatgtttac attgaatgga
      781 atcaaccatg ccaacaaata caccaacaac atgcatactg caaaaacaat tgcacttttt
      841 aaaacagcac ttcggaagcc tttaattgct gacttcattt aactacttta actcttccac
      901 ctccgctata agaatgaaac tcgccatcat acatggcatc agcactcacc ggccccacaa
      961 caaaacttcc tgtattgaca gctctgacgg taaaataaaa atactttgtt gttttacctg
     1021 cctgagtata tagattaatt ctgtcatcac ggatatcgaa ataatccggc tgtgatttac
     1081 tttttatcca agtcatttct ctgtcgtcca caattcgtgg attttcaatt tcaaatcctg
     1141 aaggaattaa atcagtaagt acaacatttt ctacttcaat gccggtagga gaacttaatg
     1201 ccaactgcac tacaatcaaa tcgccttgct tgaaattgtt attaatttgc tgaccaaagc
     1261 gattgaagta acttctcctg acattcaatt tactatcctc atctttcaac cttaaacttt
     1321 ctttaattcc actcacttca agaaaataat acaactgacc tttgccttct gtccgtattt
     1381 caattttttt tcctgcaaga ttgctattta tcactaaatc atttccatta aaggtgccta
     1441 tttgttttcc atcagcaaat acagttgcac gaacatttgc agaagtgctg cgtttagcaa
     1501 acttgcccaa tgcaagtaat gcaaatgcac gttcctgagt gcttaaccat ttttctcctc
     1561 ttatcaattg cgaaagtgtg cgtgccatag ttggaatctg tgcattgtcg tggtctgttt
     1621 ccaacaagca gtttaatgcc aatgcctgat ctctgatgta ggatgagaaa ttacctgaca
     1681 gactacgttg tga
//
LOCUS       DAORQT010000323         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16047_length_1639_cov_14.364899, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000323 DAORQT010000000
VERSION     DAORQT010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16047_length_1639_cov_14.364899"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..505)
                     /locus_tag="PK134_08570"
     CDS             complement(134..505)
                     /locus_tag="PK134_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRB38942.1"
                     /translation="MGTFKDLIVYKKAFALAMEIFEMTKSFPKEEKYSLIDQIRRSSR
                     SVCANLGEGYRKRRYVAHFVAKTTDSDMENTETQVWLDFSFACKYITDEIYNDKQAKS
                     EEVGRLLQDMIINPKKYGSVQ"
     gene            complement(552..>1639)
                     /gene="murD"
                     /locus_tag="PK134_08575"
     CDS             complement(552..>1639)
                     /gene="murD"
                     /locus_tag="PK134_08575"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRB38943.1"
                     /translation="KRIQVISEMELAYRFKGESKIIAITGSNGKTTTTAMTYHICHEA
                     GLDCAMVGNIGDSFARRIAEDPSALYVVEVSSFQLDDIITFRPDIAILTNITEDHLDR
                     YDYKFENYIKSKFRIIMNQQATDHFIYCCDDEITMKYLNQFNIQSNQLPISMKKELPN
                     GAFIKDGDMYVRTGQDFTTMSVFDFALKGKHNQYNTMAACVAATTLDIRKEKIRDAVQ
                     NFQSLEHRMEHVATVRGVEFINDSKATNVNSTWYALESMTKPTILILGGVDKGNDYSL
                     IQELVDEKVKAIICLGTDNRKIHEAFGNKVSTIVNTSTSAEAVHAAFHFADKGDVVLL
                     SPACASFDLFKNYEDRGNQFKQVVKDL"
BASE COUNT          444 a          348 c          230 g          617 t
ORIGIN      
        1 cccagataac cttatcgcct tttgttttgc tgataagatt attggtattg aaggaattcc
       61 tcaaaccact aaatcgtatg tcttttgcta catccatttt tgaagttggc agttggcaat
      121 ttgcagttcc ctgctattgc acacttccat atttttttgg gtttataatc atgtcctgta
      181 ataatcttcc tacttcttca cttttagctt gtttgtcatt atatatttcg tctgttatgt
      241 acttacaagc aaaactaaaa tccaaccaaa cctgtgtttc agtgttttcc atatcactat
      301 cagttgtttt agcaacaaaa tgagctacat atcttctttt cctataccct tcgcccaaat
      361 ttgcacatac tgaccttgaa ctcctcctta tttgatctat caaagaatac ttttcttcct
      421 ttggaaaact tttagtcatt tcaaaaatct ccatagctaa tgcaaatgct tttttataaa
      481 ctattaaatc cttgaatgtg cccatattac tttagcgttt ctaactgcaa attgccaact
      541 gctaactttt ctcacaaatc ttttactact tgtttaaact gattgcctct gtcttcataa
      601 ttcttaaaca aatcaaaact tgcacaagca ggacttaata aaacaacatc gcctttgtca
      661 gcaaaatgaa aagctgcatg tactgcttct gctgatgttg acgtattaac tatcgtactc
      721 accttgtttc caaatgcctc atgtatcttt ctattatctg ttccaaggca aatgattgct
      781 tttacttttt catccactaa ttcttgaatt agtgaataat catttccttt atcaacaccg
      841 cccaaaatca aaatagttgg cttggtcata ctttccaaag cataccaagt agagtttaca
      901 ttggttgctt tgctgtcatt aataaattca actccccgaa ctgttgctac atgttccatg
      961 cgatgctcaa gactttgaaa attttgcacc gcatcacgaa ttttttcttt ccggatgtcg
     1021 agagttgttg ctgctacaca agctgccatt gtattatact gattatgctt tccttttaaa
     1081 gcaaaatcaa atacactcat tgttgtaaaa tcctgacctg ttcttacgta catatccccg
     1141 tctttaataa atgcgccgtt tggtaattct tttttcatgc tgattggcag ttggttagat
     1201 tgaatattga attggtttag gtatttcatt gttatttcat catcgcagca atagataaaa
     1261 tggtctgttg cctgctgatt cattattatt cgaaacttac tttttatata attctcaaat
     1321 ttgtaatcgt aacggtctag atggtcttct gtaatatttg tcaggatggc aatgtccggt
     1381 ctgaaggtta ttatatcatc caattgaaaa ctgctaacct ctaccacata aagtgccgat
     1441 ggatcttcag ctattcttct tgcgaatgaa tcaccgatat ttcctaccat cgcacaatca
     1501 agaccagctt cgtgacaaat atgataagtc attgctgttg tcgttgtctt tccattactt
     1561 ccggtgattg caattatttt gctttcgcct ttaaaccggt aagccaactc catttcactt
     1621 atcacctgta ttctttttt
//
LOCUS       DAORQT010000324         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16049_length_1639_cov_8.688131, whole genome shotgun sequence.
ACCESSION   DAORQT010000324 DAORQT010000000
VERSION     DAORQT010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16049_length_1639_cov_8.688131"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PK134_08580"
     CDS             <1..1006
                     /locus_tag="PK134_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRB38944.1"
                     /translation="LTEYRTQNRGGRGSIGSSTRDEDFVEHLFVATNHNYILLFTQKG
                     RCFWLKTYEIPEGNKQFKGRAIQNLISIEPDDKVFAFINVKTLEDEEYINNNYIVMCT
                     RQGVIKKTPLEAYSRVRQNGINAIVINDGDELIEAKLTNGNNEILLAVKSGRAIRFNE
                     NTVRTVGRNSIGVRGVSLDHAKDEVIGMVCIKNATEETILVVSEKGYGKRSDLEDYRV
                     TNRGGKGVKTMNITEKTGDLIAIKSVKEDDDLMIINKSGITIRMEVKDLRVMGRNTQG
                     VKLIRLDDDDSIASVAKIDMEITGNGNQKENGNDTEENPTIENGEQNNNQTEENNNPE
                     "
     gene            1031..>1639
                     /locus_tag="PK134_08585"
     CDS             1031..>1639
                     /locus_tag="PK134_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38945.1"
                     /translation="MKRILTVAFISLTLGGFAQRAQVQSAYSYLKYEQLDKAKECIDI
                     AVNNENTMNYDKAWYYRGLIYQALYKNEKFANLSNDPLNEALRSYNKALELNPKFEYA
                     DDISEKKKILAQQFADMGYVNYNLKNYAGALTAYEGVVSIMPNDTSSYFNAALCAERA
                     GNAAKAKQYYNKLDEMQYKDAKMYHSYAQLYLSEGDTAKALEV"
BASE COUNT          593 a          286 c          344 g          416 t
ORIGIN      
        1 gctgacagag taccgcacac aaaaccgtgg tggtcgtggt tcaattggca gcagcacacg
       61 tgatgaagat tttgtagagc atttatttgt agcaaccaat cacaactaca ttctgctgtt
      121 tactcaaaaa ggacgctgtt tctggctgaa gacttatgaa attcctgagg gcaacaagca
      181 atttaaagga cgtgctatcc aaaacttaat cagcattgaa cctgatgaca aagtgtttgc
      241 tttcatcaat gttaagacat tggaagacga agagtatatc aacaacaatt acattgtaat
      301 gtgtacccgt cagggagtga taaagaaaac accgcttgag gcctactcac gtgtacgtca
      361 gaatggtata aatgccatcg tgataaatga tggtgatgaa ttgattgaag caaaacttac
      421 caatggcaac aatgaaattt tacttgctgt taagtccggt cgtgctattc gatttaatga
      481 gaacaccgtt cgtactgtgg gtcgtaattc tattggtgta agaggtgttt ctcttgatca
      541 tgctaaagat gaagtgatag gcatggtttg tataaaaaat gcaacggaag aaaccatctt
      601 agtagttagt gaaaaaggtt atggaaaacg cagcgacctg gaagattatc gtgtaacaaa
      661 ccgtggcggt aaaggtgtta agaccatgaa cattaccgaa aaaacaggcg acctcattgc
      721 cataaagagt gttaaagaag atgatgatct gatgatcatc aacaaatccg gcataacaat
      781 tcgtatggag gtaaaagatc ttcgcgttat gggacgaaac acacagggcg taaagcttat
      841 tcgattggat gatgatgact ccattgcatc ggttgcaaaa attgatatgg aaattacagg
      901 taacggtaat caaaaggaga acggcaacga tacagaagaa aatccgacta ttgagaatgg
      961 cgaacaaaac aataatcaaa cagaagaaaa caataatcca gaataattat taatcaaaca
     1021 atttagcaaa atgaaaagaa tactaacagt tgcatttatc agtttaactc ttggtggatt
     1081 tgcacagcgt gcacaggtac aatcggctta cagctatctt aaatacgaac agttagacaa
     1141 agccaaagag tgtattgaca ttgccgtaaa caacgagaac accatgaact atgacaaagc
     1201 atggtactat cgcggactga tttatcaggc actttataag aatgaaaaat tcgctaatct
     1261 gtcaaacgat ccgcttaacg aagctttacg ttcttacaat aaagctttag agctgaaccc
     1321 aaaatttgaa tatgctgacg atatttcaga aaagaaaaaa atattagctc agcaatttgc
     1381 cgacatgggt tatgtaaatt acaatctgaa aaactatgca ggtgcattga cagcttatga
     1441 aggagtagtt tctatcatgc ctaatgacac atcatcatat ttcaatgcag cattgtgtgc
     1501 agagcgtgca ggcaatgcgg ctaaagcaaa acagtactac aacaaattag atgagatgca
     1561 atataaagat gcaaaaatgt atcacagcta tgcacaactg tatctttccg aaggcgacac
     1621 agcaaaagca cttgaagta
//
LOCUS       DAORQT010000325         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16104_length_1636_cov_10.096774, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000325 DAORQT010000000
VERSION     DAORQT010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16104_length_1636_cov_10.096774"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..>1636)
                     /locus_tag="PK134_08590"
     CDS             complement(15..>1636)
                     /locus_tag="PK134_08590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRB38946.1"
                     /translation="FVLAGAKLNDADKAKVANINSRLATLFTQFSKNQLADENEKYIE
                     IKNESDLAGLPDDIKSAYAKNAKERGKSTWTVANTRSAVEPFLTFSNNRELRKQVWTM
                     FINRGDNGDANDNNKIIPEILKLRYERAKLMGFATFAHQKLSDKMAKTPEATMKLMES
                     VWPAAIARVKEEVADMQAIADKENAGIKIEPWDYRYYAEQVRKSKYDLDQNEVKQYLQ
                     LEKLREGMFFVAGELFNLSFTQVHDVPVFHPDVTVYKVEDKTTHKLKGIWYFDPYART
                     GKRSGAWMTAYREQEKLSGNVTTIVSNNCNYIKGSGNDPVLISWDDATTLFHEFGHAL
                     HGLCSDVTYPTISGTNVARDYVEFPSQVLERWLPTSEVLQKYAINAKTGQPLPQSLLE
                     KLNKSSKFNQGFETTEYLSSALVDMKIHLAGDKVTDPDKFEKETLAALNMPSEIVMRH
                     RLPQFGHIFSSDEYAAGYYSYLWSDVISADATEAFTEAGSLYDKKTAEKLYRNVFSVG
                     ASIDESIGYRNFRGHDPDVKALMRSRGFPEK"
BASE COUNT          467 a          338 c          281 g          550 t
ORIGIN      
        1 aagccctcca aggcttattt ttcaggaaag cctctgctgc gcataagtgc tttaacgtca
       61 gggtcgtgac ctctgaaatt acgatagcct atactttcgt caattgaagc accaacacta
      121 aacacattac gataaagttt ttcggcagtc tttttatcat ataaactacc tgcttctgta
      181 aatgcttcag ttgcatcagc gctgattaca tctgaccata gataactgta atagcccgct
      241 gcatattcat cacttgaaaa aatatgccca aattgtggca gtcggtgacg catcacaatt
      301 tccgaaggca tattcagtgc agctaaggtt tctttttcaa atttatcagg atcagttact
      361 ttatcacctg caagatgtat tttcatatct accaatgcac tcgacagata ttctgttgtt
      421 tcaaatccct gattgaattt tgaagatttg ttgagttttt cgagcagact ttgtggaaga
      481 ggttgtccgg ttttagcatt tattgcatac ttctgcaaca cctctgatgt tggcaaccaa
      541 cgttcaagta cttgtgatgg aaactcaaca taatctctgg ccacattggt tcctgaaatt
      601 gtcggataag taacatcaga acacaaacca tgcaatgcat gtccgaattc atgaaacaga
      661 gtagtggcat catcccatga aataagtacg ggatcattac cggaaccttt tatgtaattg
      721 caattgttgg atacaatagt tgtaacattt ccactcagtt tttcctgttc gcgataggct
      781 gtcatccatg caccggaacg tttacctgta cgtgcatagg gatcaaaata ccagatcccc
      841 tttaacttat gtgttgtctt atcttcaact ttatatactg taacatcagg atgaaaaaca
      901 ggaacgtcat gaacctgagt aaatgaaaga ttgaataatt ctcctgcaac aaaaaacatt
      961 ccttctctaa gtttctccaa ctgaagatat tgtttcacct cattctgatc gagatcatat
     1021 tttgattttc ttacttgctc tgcgtagtag cgataatccc atggctcaat tttaattcct
     1081 gcattttctt tatcggcaat tgcctgcatg tcggcaactt cttcttttac tctggcaata
     1141 gcagcaggcc aaacagattc catcaatttc atagtagctt caggtgtttt agccattttg
     1201 tctgaaagtt tctgatgtgc aaatgttgca aatcccatca gctttgctct ttcataacgt
     1261 aattttaaaa tttcaggaat aatcttgttg ttatcgttgg catcgccatt gtcgccacgg
     1321 tttataaaca ttgtccaaac ttgtttacgt aattcgcgat tgtttgaaaa agttaaaaaa
     1381 ggctcaacag cagaacgtgt attggcaaca gtccatgttg atttaccacg ctcttttgca
     1441 ttttttgcat acgctgattt tatgtcatca ggtaatcctg ccaaatcgga ttcattttta
     1501 atttctatat atttttcatt ctcatctgct aattgatttt tagaaaattg agtaaacaaa
     1561 gtggccaatc gtgaattgat attagccacc ttagctttat cagcatcatt aagtttagca
     1621 cctgcaagga caaaat
//
LOCUS       DAORQT010000326         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16194_length_1630_cov_6.827937, whole genome shotgun sequence.
ACCESSION   DAORQT010000326 DAORQT010000000
VERSION     DAORQT010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16194_length_1630_cov_6.827937"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..994)
                     /gene="prmC"
                     /locus_tag="PK134_08595"
     CDS             complement(131..994)
                     /gene="prmC"
                     /locus_tag="PK134_08595"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRB38947.1"
                     /translation="MMRLFSFATMTRKEIINVFQADVELIYDKGESSAITKLVMEYIF
                     GLNYKSNTHENVNVSQETLLRQYLERLRNYEPVQYILGQAWFCNHIYKVNSSVLIPRP
                     ETEELILLIKQNLKSSHIGSIIEIGTGSGCIAIELSLLFAQATVIATDISENALEMAR
                     KNAESLRASVSFINESLFVPSSKTIQTKVDLIVSNPPYITQQEATQMHPNVLQWEPHT
                     ALFVKENPLEFYIAIASLATNILNDKGYVFLELNPDYAFQVAQCFTEKGFTQVEIVKD
                     ISGKERFLKVQ"
     gene            1018..1584
                     /gene="ribD"
                     /locus_tag="PK134_08600"
     CDS             1018..1584
                     /gene="ribD"
                     /locus_tag="PK134_08600"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRB38948.1"
                     /translation="MVMTFDETIMNRCLELAKKALGQTMPNPMVGSVLVFENKIIGEG
                     YHHRAGEAHAEVNAINSVENKNLIPLSTLYVSLEPCSHYGKTSPCCERIVQEGIKKVV
                     VGTLDTTAKVNGKGISYLQQNNVEVATPMLNDACRFLNRRFFTFHEKQRPYVILKWAE
                     SADGFIAPENAQQQYAISNTYSKTLSHK"
BASE COUNT          530 a          295 c          290 g          515 t
ORIGIN      
        1 atattgttgc agagggcaca ccattacaaa tcagtaaaaa cgccaacagc attacaggaa
       61 cttttttgaa aaaagaaatg gaatggcaca ataaagtcag tgcggaagtc tgattttatt
      121 ttttacttaa tcactgtact tttaaaaaac gttccttacc tgaaatatct tttactattt
      181 caacttgggt aaaccctttc tctgtaaaac actgtgctac ctgaaatgca taatcaggat
      241 taagctctaa aaaaacatag cctttgtcat ttaatatatt cgttgcaaga gatgctattg
      301 caatataaaa ttcaagcgga ttttccttta caaataaagc tgtatgaggc tcccactgca
      361 atacattggg atgcatttga gtagcttctt gttgcgtaat gtaaggcgga ttagaaacaa
      421 ttaaatctac tttagtttga atcgttttgc tgcttggcac aaatagagat tcgttgatga
      481 atgaaacaga agctcttaaa ctctcagcat tctttcttgc catttcaagt gcattttctg
      541 aaatatctgt tgcaatgact gttgcctgtg caaataataa tgacaactca atagctatgc
      601 aaccggagcc tgtacctatt tcaattattg acccaatatg tgatgacttc agattttgct
      661 tgataagcag aattaactct tcagtttccg gtcgtggaat aagtacgctt gagttaactt
      721 tatagatatg attacagaac catgcctgac ctaaaatata ttgtactggc tcgtaatttc
      781 gtaaacgctc caggtactga cgaagtaagg tttcctgtga cacattaaca ttttcatgag
      841 tatttgattt gtagtttaag ccgaaaatat attccataac taacttggta atggcgctac
      901 tttcaccttt gtcatagatt aattcaacat ctgcctgaaa aacattaata atttcctttc
      961 ttgtcattgt tgcaaagcta aataaacgca tcatttattt taattttgca gcaacaaatg
     1021 gttatgacct ttgacgaaac aataatgaat cgttgtcttg aactagccaa gaaggcctta
     1081 ggacaaacta tgcctaatcc tatggtgggc agtgttcttg tttttgaaaa taaaattatt
     1141 ggtgaaggtt atcatcatcg cgcaggagaa gcacatgcag aagtcaatgc aataaacagt
     1201 gtagaaaata aaaaccttat tcctctttca acgctttatg tttcattaga gccatgttcg
     1261 cattacggca aaacgtcacc ttgctgtgag cgtattgttc aggaaggaat taagaaagtt
     1321 gttgttggca ccctcgatac tacagcaaaa gtaaatggca aaggcataag ttatcttcag
     1381 caaaataatg ttgaagtcgc tacaccaatg ctcaatgatg catgtcgttt tttaaacaga
     1441 aggtttttta cttttcatga aaaacaaaga ccttatgtca ttttaaaatg ggcagaaagt
     1501 gctgatggct ttattgcacc tgaaaatgcg cagcaacaat acgctataag taacacctat
     1561 agcaaaacat tatctcataa ataggacgac cggcagtgtt ggaaggctct ctgtcatcat
     1621 tccagcgaaa
//
LOCUS       DAORQT010000327         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16581_length_1606_cov_6.473888, whole genome shotgun sequence.
ACCESSION   DAORQT010000327 DAORQT010000000
VERSION     DAORQT010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16581_length_1606_cov_6.473888"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PK134_08605"
     CDS             <1..496
                     /locus_tag="PK134_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRB38949.1"
                     /translation="SKTLSHKWRTEEQAILVGTKTAILDNPQLNPRLWKGSTPIRVVI
                     DKNLDVPATHHLLDGSVKTILINQKKENRANNIDYIKINFQENPATQILKVLYQQNIQ
                     SVIIEGGAKTLQYFIQSNLWDEARVFTATKQLISGIKAPVFLISPSEITNIEDDTLKI
                     FYNV"
     gene            493..1353
                     /locus_tag="PK134_08610"
     CDS             493..1353
                     /locus_tag="PK134_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HRB38950.1"
                     /translation="MTVTVFMAVLFASLLFVILKLFDRFKVSNIHGLTFNYFTAGICY
                     TLYSKQTYSSISGFAASYFPYYLLSGSLFIGVFYLTALSSQKNGVASTTIASKMSLVI
                     PALLAVFLYNETFSFLKTIGLLMALSAVYLSARGSNNESSESGWTLPLLVFAGAGLVD
                     SSIKYTQHVFTDNAGSSGFFTLVFFSAAIIGSCVLFFSKNMPKEGSLFRNIIAGIILG
                     IFNFISLYYMVAALQEFHEDSGKAFILINSGVVLLNFLLSIILFQEKKNTQSWVGVAL
                     AVVAIIILSQ"
     gene            1354..>1606
                     /locus_tag="PK134_08615"
     CDS             1354..>1606
                     /locus_tag="PK134_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRB38951.1"
                     /translation="MFETSYKTIVGYSEGVFRDKGSRFLVFAYYVEKESECKSVLEQI
                     KKEHPKANHHCYAYRLGPYKNIFRWNDDREPSNTAGRPIY"
BASE COUNT          530 a          281 c          272 g          523 t
ORIGIN      
        1 tagcaaaaca ttatctcata aatggcgaac agaagagcag gctatattgg taggaacaaa
       61 aacagctatc ttagacaacc cacaattgaa ccccagactt tggaaaggaa gtactccaat
      121 tagagttgtc attgataaaa acttagatgt gcccgcgaca catcacttgc tcgatggttc
      181 tgtaaagaca attttaataa atcagaaaaa agaaaacaga gcgaataaca ttgactatat
      241 aaaaataaac tttcaggaaa atccagcaac tcaaattctg aaagtattat atcagcaaaa
      301 catccaatca gtaataattg aaggcggagc aaaaacactg caatacttta ttcaaagcaa
      361 tttatgggat gaagcaagag tgtttactgc aactaaacaa ttaatcagcg gaataaaagc
      421 acctgtattt ttaatttcac cttccgaaat cacaaacatt gaagacgata cattaaaaat
      481 attttacaac gtatgacagt tactgttttc atggctgtac tgtttgcctc actcctcttt
      541 gtcatactta aactattcga tcgttttaaa gtcagcaaca ttcacggact aacatttaat
      601 tattttacgg ccggtatttg ttatacctta tattcaaaac aaacctacag ttcaatttca
      661 gggtttgcag caagctattt cccctattac cttctgagtg ggagtttgtt tattggtgta
      721 ttttacctta ctgctttatc ttcacaaaag aatggtgttg cttcaacaac tattgccagc
      781 aaaatgtctt tagttatacc tgcactactg gctgttttcc tgtacaatga aactttttct
      841 tttctgaaaa ctatcggcct attaatggcg ctatcagcag tatatttatc agcaagaggc
      901 agtaacaatg aatcatcaga atccggatgg acattaccat tattggtatt tgcaggtgcc
      961 ggtcttgttg attcttcaat aaaatacaca caacatgttt ttacagacaa tgcaggcagt
     1021 agtggcttct tcacattagt atttttttct gctgcaataa ttggcagttg tgttcttttc
     1081 ttcagtaaaa atatgcccaa agaaggaagc cttttccgaa atatcatagc aggaattatt
     1141 ttaggtatct ttaatttcat ttctctttat tatatggttg ctgcacttca ggaatttcat
     1201 gaggacagtg gtaaagcatt tattttaatt aactcaggtg ttgtattgtt aaacttttta
     1261 ctttcaatta ttttatttca agaaaagaaa aatacacaaa gttgggttgg tgttgcactt
     1321 gcagtcgtag caattattat attgtcgcaa taaatgtttg aaacgagcta taaaacaatc
     1381 gttggttatt ctgaaggcgt tttcagagat aaaggcagca ggtttctagt ttttgcctac
     1441 tatgttgaaa aggaaagtga atgtaagtct gttcttgaac aaataaaaaa agaacatcca
     1501 aaagccaatc atcattgtta tgcttatcgt ttaggacctt ataaaaatat ttttcgctgg
     1561 aatgatgaca gagagccttc caacactgcc ggtcgtccta tttatg
//
LOCUS       DAORQT010000328         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16607_length_1605_cov_4.330323, whole genome shotgun sequence.
ACCESSION   DAORQT010000328 DAORQT010000000
VERSION     DAORQT010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16607_length_1605_cov_4.330323"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..512)
                     /locus_tag="PK134_08620"
     CDS             complement(30..512)
                     /locus_tag="PK134_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38952.1"
                     /translation="MLRIPSLASLIFICAFLQTHFVFAQQDDARKVITELTSEKYFGR
                     GYLKHGDKKAAHFIKKQFSKLGLKPVDDKHWFQHFTHPVNRFPKNVSLTTDTKNWIPG
                     KEFLIHHQSPSANGTWPIAWADSAEIISRLNNDYDSCYVITRQVFSKAVISFCCLLKT
                     "
     assembly_gap    598..671
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<672..>911)
                     /locus_tag="PK134_08625"
     CDS             complement(<672..>911)
                     /locus_tag="PK134_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38953.1"
                     /translation="SVVPPVQTARAEVFKEADQTVATMFHETETIHDKIGVAQPAFAI
                     ADKLKLSPVTDLVKAIGINEKFLFISQLFNDDSIK"
     assembly_gap    912..978
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<979..1310)
                     /locus_tag="PK134_08630"
     CDS             complement(<979..1310)
                     /locus_tag="PK134_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38954.1"
                     /translation="MTKQSITSEIQVAIEQIYRQWGVFSLDNRIPENDELDMLKSKLM
                     KLHDKINHLQLLESVYAYKQPIQEVITETVQKPITQTVIEKSETEQKITIEAPEVKTF
                     EPIIAEVT"
BASE COUNT          421 a          254 c          256 g          533 t
ORIGIN      
        1 gattatgaca gttgttatgt cataacacgt caggttttca gtaagcagca aaaagaaatt
       61 acagccttac tgaaaacctg acgtgttatg acataacaac tgtcataatc attgttaagt
      121 cttgaaataa tttctgcaga atctgcccag gctataggcc atgttccatt ggcagatggc
      181 gattgatgat gtattaaaaa ctctttcccg ggaatccaat tttttgtgtc tgtagttaga
      241 cttacatttt ttggaaaacg gtttacagga tgtgtaaaat gctgaaacca atgtttatca
      301 tcaacaggtt ttaatccaag tttgctgaat tgttttttta tgaaatgtgc agctttctta
      361 tcaccatgtt ttaaatagcc acgaccaaaa tatttttctg aagttaattc agtaataact
      421 tttcgcgcat catcttgctg tgcaaaaaca aagtgtgtct gtagaaatgc acaaataaaa
      481 atcagcgatg caagagaagg tattcgcagc attacataaa tcgcctttgt agcaaatcta
      541 caaactgtct gcaggcatca ctgtttttgt ctatttgatt tcctgaagtt atttcagnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn ntattttata gaatcatcat taaacaactg actgataaat aaaaattttt
      721 cgtttatgcc tattgcttta accaagtccg taactggtga tagttttaac ttatctgcta
      781 tggcaaaagc cggttgcgcc acaccaattt tatcgtgtat ggtttctgtt tcgtgaaaca
      841 tggttgctac agtttgatct gcttctttaa atacttcagc tcttgcagtt tgtacaggag
      901 gaaccacaga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnntc agtgacttca gctattatcg gttcaaaggt ttttacctcc
     1021 ggtgcttcaa tggttatttt ttgttccgtc tcagattttt caatgacggt ttgtgttatt
     1081 ggtttttgca ctgtttctgt aattacttcc tgaataggct gcttataagc ataaacagat
     1141 tcaagtaatt gaagatgatt gattttatcg tgcagcttca tcagtttgct tttcagcatg
     1201 tctaattcat cattttcagg aatgcggttg tctaatgaaa aaacgcccca ttgcctgtaa
     1261 atttgttcta ttgcaacttg aatttcgctg gtgatagatt gctttgtcat gaaattgtgt
     1321 ttttatgcta acgctaaata aattaattca tttttgattt ggaaaattta tgggtaatct
     1381 cagaaaattt tcatgagcaa aattaattgt catactactg tcaacattgt tggttgactg
     1441 atatttatca acataacttt tccatgctgc aagtcgctac ttttactttt gcagctgtga
     1501 aaaaacccat actcctattc acattttttc tattgtctgt aacgcttttt gctgccgatc
     1561 aggtattatt tatcaaagac aaatccaatg gcaagccggt aaatg
//
LOCUS       DAORQT010000329         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16613_length_1605_cov_3.144516, whole genome shotgun sequence.
ACCESSION   DAORQT010000329 DAORQT010000000
VERSION     DAORQT010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16613_length_1605_cov_3.144516"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..902)
                     /locus_tag="PK134_08635"
     CDS             complement(105..902)
                     /locus_tag="PK134_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB38955.1"
                     /translation="MKGLIKILLLIFFVSCSNVPLTERKQINLLPESQMVAMALTSYS
                     DFLKEHPPVVNSNADAQMVKRVGERVKDAVVQYMRDHNLYDRIKDYKWEFNLVDSKDV
                     NAWCMPGGKVVVYSGLLPVTKNEESLALVMGHEIAHAIARHGNERMSQQLIAATGGLA
                     LDVALMNKPAETRQLFNTAYGVGANLGVLLPYSRLHETEADKLGLIFMAMAGYNPAIA
                     PDFWKRMAAVSGNKVPEILSTHPSSETRIHDLNKFMPTAMKYYKPRP"
     gene            complement(983..1255)
                     /locus_tag="PK134_08640"
     CDS             complement(983..1255)
                     /locus_tag="PK134_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRB38956.1"
                     /translation="MSVKQAEEIKESKKSQSNDHHIVLYNDDVNTFDFVIDSLMEICN
                     HEREQAEQCSIIVHNNGRCSVKDGSVKTLRPMCKALVERGLTAEVE"
     gene            complement(1363..>1605)
                     /locus_tag="PK134_08645"
     CDS             complement(1363..>1605)
                     /locus_tag="PK134_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38957.1"
                     /translation="VQKEYVESSVTANQKTILKPAVALINISYAETSDQATAKVLENG
                     NAPKVVNLNLKEEAIPVSIRRQHNSVIGKYIPVVIN"
BASE COUNT          491 a          326 c          245 g          543 t
ORIGIN      
        1 cggctactaa actatttaag ccggtctggt aatgtgaaaa atattctgga aattgtaaat
       61 gttaaaataa gatgtgtaga aattcctttc caaaaaaaca ataactatgg acgaggttta
      121 tagtacttca ttgctgtagg cataaactta ttcaaatcgt gaatacgtgt ttcgctactt
      181 gggtgtgtgc ttagtatttc aggaacttta ttacccgata ctgctgccat tcttttccaa
      241 aaatcaggtg ctattgcagg attataacct gccatcgcca taaatattaa tcccagttta
      301 tcggcttctg tttcatgcaa gcgtgagtaa ggtaatagta cgccaaggtt tgcaccaaca
      361 ccataggctg tattaaacaa ctgtcttgtt tctgcaggct tattcatcaa tgcaacatct
      421 aatgccagac cacctgttgc tgctatcaat tgctgactca ttctttcatt tccatggcga
      481 gctatagcat gtgcaatttc atgccccata accaaggcaa ggctttcctc attttttgtt
      541 acaggtaata atcccgaata gacaacaact ttacctccag gcatacacca ggcatttaca
      601 tctttactgt caacaagatt aaattcccac ttgtagtctt ttattctatc gtatagatta
      661 tgatcgcgca tgtattgaac aacggcatcc ttaacacgct cacctactct tttaaccatt
      721 tgcgcatctg cattactatt tactacagga ggatgttctt taagaaaatc gctgtatgat
      781 gttagggcca tagcaaccat ctgcgattcc ggtagaagat ttatttgttt tcgctctgtt
      841 aacggaacat tactgcatga aacaaaaaag atcagcaaca atatttttat taaacctttc
      901 atgtgattta cgctttaaac taaaacttaa taattctgct ttcggtatgc atacaaaata
      961 aattccgaat gaaaatcctg acttattcta cctcggctgt aagtccacgt tcaaccaaag
     1021 ccttacacat tggcctcaac gtctttacag agccatcttt aacagaacac ctgccattat
     1081 tatgaactat aattgaacac tgctctgctt gttctctttc atgattacaa atttccatta
     1141 aagaatctat tacaaaatca aaagtattca catcatcatt atacaatacg atatgatgat
     1201 cgtttgattg cgatttttta ctttctttaa tttcctcagc ttgttttacc gacatgatgt
     1261 ttctttgttg aacagtgcaa taataacaag caaaatcaaa tttcaaaaat caatggagaa
     1321 aaatcaggaa aaagaccgat ttcttcataa aaagaaaaat atttaattga tgactacagg
     1381 aatgtattta cctattactg agttgtgttg tcgcctgata ctcacaggta tagcttcctc
     1441 ttttagattt aaatttacca cttttggagc atttccattc tctaaaactt ttgctgttgc
     1501 ctgatcactt gtttcagcat aactaatatt tattaatgcc actgcaggct tcagaattgt
     1561 tttctgattg gcagtcactg aagattctac atattccttt tgaac
//
LOCUS       DAORQT010000330         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16835_length_1591_cov_11.388021, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000330 DAORQT010000000
VERSION     DAORQT010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16835_length_1591_cov_11.388021"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1591
                     /locus_tag="PK134_08650"
     CDS             <1..>1591
                     /locus_tag="PK134_08650"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38958.1"
                     /translation="MAQNCNANFQYFAGPGGVVTFMDSSFASGNITGYTWTFGDGTSG
                     TGQQVSHTYNGVGPYTVCLFITTSNGCSDSTCALVSLNPCNISGVILYDSLQQTLTVT
                     ASGGVAPYTYNWTNGQTGMSISVGNPGMYCCVITDANGCAYTACYMVGGSGCSAAFIT
                     NIMGNVVVFGNNSQSFASLLWNFGDGTTSVATSPTHTYAQAGTYYPCLTVYDGNGAIC
                     SQYCDTIVIVPQAGNTVICGNVFQDMNANGFNDNEPGFANTPVMIWGSGMQYTAMPDS
                     NGNYSINVPPGTYNIVYCVQQPYSLTLPLDSGGCGFYQVTIAAGDTICGFNFGVSLNS
                     SSIEGYVFVDSNTNGIKDAGEAGIAYQPVQIGNLTSYTDLTGKYERYVPIGTYTVSYT
                     PTGVYASYPLTTASSNSVSVVGVGITYQGGNYGLDLPAGSTNLAVNLLPHTTVTPGFP
                     AWYDIQVCNIGINPTGATVTMNYDPGLTYTFGSPVPTSVNTTAHVITWNLPTMTSGQC
                     LYIQADFSASTSYQIGDPTFE"
BASE COUNT          443 a          332 c          328 g          488 t
ORIGIN      
        1 gtatggcaca aaattgtaat gcaaactttc aatactttgc cggacccggt ggagtagtaa
       61 cttttatgga ctcaagtttt gcatcaggca atatcaccgg ttatacctgg acttttggtg
      121 atggcacatc aggaacaggt cagcaagtat cgcatactta taatggtgtg ggtccttaca
      181 cagtatgttt atttattaca acatctaatg gttgcagcga ttctacctgc gcattagtta
      241 gtttaaaccc ctgtaatata tccggtgtca ttctttatga ttcactgcaa caaacattaa
      301 cagttacagc atcaggcggt gtagcacctt acacctacaa ctggacaaat ggtcaaacag
      361 gaatgagcat ttctgttggt aatccaggaa tgtattgctg tgtgattacc gatgctaacg
      421 gctgtgccta tacagcatgc tatatggtgg gtggctccgg ttgcagtgca gcatttatca
      481 ccaacattat gggtaatgtt gtggtgtttg gtaacaactc acagagtttt gcatcactac
      541 tctggaactt tggtgatggc accacttctg ttgctacatc accaacacac acttatgctc
      601 aggcaggaac ctattatcct tgtctgacag tttatgatgg caacggtgct atatgttcac
      661 agtattgcga tacgattgta attgtaccac aagcaggcaa tacagttatt tgcggcaatg
      721 tctttcagga catgaatgcc aatggattta atgataatga accgggcttt gccaatacac
      781 cggtaatgat ttggggatcc ggtatgcaat acactgcaat gcccgattca aatggtaatt
      841 acagcatcaa tgttcctccc ggaacttata atattgttta ctgtgttcaa caaccttaca
      901 gtcttacctt accacttgat tcaggaggtt gcggttttta tcaggtaaca attgcagcag
      961 gtgatactat atgtggattt aattttggtg tttctctcaa cagttcttca atagaaggtt
     1021 atgtttttgt tgacagcaac actaatggaa tcaaagatgc aggcgaagca ggtattgctt
     1081 atcaacctgt gcagattgga aaccttactt cctacactga tttaacagga aaatatgagc
     1141 gttatgttcc gattggaact tatacagttt cctatacgcc aacaggagtt tatgcatctt
     1201 atccattgac aactgcaagt agtaacagtg taagtgtcgt aggcgttggt attacttatc
     1261 agggtggaaa ctatggtctt gatttacccg caggttcaac aaatcttgca gtaaatttat
     1321 taccgcatac tactgttaca cccggcttcc ctgcatggta cgatattcag gtttgcaaca
     1381 ttggcatcaa cccaacaggt gcaacagtta ctatgaacta cgacccggga ttaacttata
     1441 catttggtag tcctgtacca acatctgtaa atacaactgc gcacgtcatt acctggaatc
     1501 ttccaacaat gacttcagga caatgcctct atatacaggc cgacttcagt gcttcaactt
     1561 cttatcagat tggcgaccca acgtttgagt t
//
LOCUS       DAORQT010000331         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16880_length_1588_cov_12.038487, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000331 DAORQT010000000
VERSION     DAORQT010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16880_length_1588_cov_12.038487"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1483
                     /gene="odhB"
                     /locus_tag="PK134_08655"
     CDS             251..1483
                     /gene="odhB"
                     /locus_tag="PK134_08655"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRB38959.1"
                     /translation="MKVEIKVPSPGESITEVQIARWIKKDGDYVERDEEIAEIDSDKA
                     TLSLVAEADGVLKTLVGEGENIAVGSVVCTIDTDAKPVAKAEPKKEEKPKEEKPKEVK
                     PTVTVASAKAEGYAAGTPSVAAAKMMKENNVKSESVQATGKGGRITKGDVLQSLQTPA
                     ATQAAPQAREQRREKMTMLRKKLSQRLVAVKQETAMLTTFNEVDMSAIMDIRKKYKDV
                     FKEKHGVGLGFMSFFTKAVCEALKSFPAVNAQIDGEEILYHNYCDVGIAVSAPKGLMV
                     PIIRSAETLSLAQIEQQVIDLATKARDSKLTIDEMTGGTFTITNGGVFGSMLSTPIIN
                     PPQSAILGMHNIVERPVAVNGEVVIRPVMYVALSYDHRIIDGRESVGFLVKVKQMLEE
                     PAKMLFEGNDPIKVLLHL"
BASE COUNT          526 a          248 c          383 g          431 t
ORIGIN      
        1 acagaacgaa tatggtgagc agcgtgtatt ttattttttt ggaagacatg gttgagccga
       61 cagaacaaaa atataaaggt gtgttgtttt atttctgaaa tattaatttg cttactaaca
      121 attgcaattg aattacggaa ctgatgcagg tcagtgcgta tgaaggcaga agtgtgttta
      181 tcttttataa ctttgcacaa agagtgtttg tcaggattta taaaaatgct gagttttaaa
      241 aaagtaaata atgaaagtag agattaaagt tccaagtccg ggtgagagca ttacggaagt
      301 acagattgca cgctggataa aaaaagatgg cgattatgtt gagcgcgatg aagagattgc
      361 agaaatagat tcggacaagg caacgctgtc gttagttgcc gaagctgatg gtgttttgaa
      421 aacccttgtt ggcgaaggag agaatattgc agttggctct gttgtgtgta cgatagatac
      481 agatgcaaag ccggttgcaa aagcagaacc taagaaagaa gaaaagccaa aggaagaaaa
      541 accaaaagag gtaaaaccta ctgtgacagt tgcatcagca aaagcagaag gctatgctgc
      601 aggcacaccg tctgttgctg ctgctaagat gatgaaagaa aataatgtga aaagtgaaag
      661 tgtgcaggca acaggtaagg gcggacgtat tacaaaagga gatgtgttgc agtcgttaca
      721 aacacctgct gccacacaag cggcaccaca ggcacgtgaa caacgcagag aaaaaatgac
      781 aatgctgcgt aaaaaacttt cgcagcgttt ggttgcagta aaacaggaaa cggcaatgct
      841 tacaactttt aacgaggttg atatgagtgc cattatggac atcagaaaaa aatataaaga
      901 tgtttttaaa gaaaagcatg gagtaggttt aggctttatg tcgtttttta cgaaagctgt
      961 ttgcgaggcg ttaaaatctt ttcctgcagt gaatgcacaa atagacggag aagaaatttt
     1021 gtatcacaac tattgcgatg tgggtattgc agtgagtgcg ccaaaaggat tgatggttcc
     1081 gattatccgc agtgcagaaa ctttgtcgct tgcacagata gaacaacagg tgatagacct
     1141 tgctaccaaa gcccgcgaca gtaagctgac gatagatgaa atgacgggag gaacttttac
     1201 gataactaat ggtggtgtgt ttggttcaat gctttcaaca ccaattatta atccgccaca
     1261 gagtgccatt ttaggaatgc ataatattgt tgagcgtcct gttgcagtga atggagaggt
     1321 agttattcgt cctgtaatgt atgttgcatt atcttatgat catagaataa ttgatggcag
     1381 agagtctgtt ggatttttag ttaaagtgaa gcagatgctt gaagagcctg caaaaatgtt
     1441 gtttgaaggc aacgacccaa ttaaagtatt actacatttg taaccacttt tcttttacca
     1501 ataaacaaaa agaggctgcc tgaatcaggc agcctctgct attttatttt gtgatgtagt
     1561 tactccacaa ctttcatttc gtcaacaa
//
LOCUS       DAORQT010000332         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16881_length_1588_cov_7.519243, whole genome shotgun sequence.
ACCESSION   DAORQT010000332 DAORQT010000000
VERSION     DAORQT010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_1588_cov_7.519243"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PK134_08660"
     CDS             complement(<1..1397)
                     /locus_tag="PK134_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRB38960.1"
                     /translation="MKTSHSTQRLVLLITIALVAITYIGRLFYLQVIEDKYKLSADEN
                     VLRRIIDYPSRGLVYDRNGKLLVFNQTVYDLMITPREVKEMDTAEFCSIIKISIEDFR
                     KKFQKAKNYSPYRSSIFEKQISAETYGTLQEKLFKYKGFSVQPRTIRKYPYNTAAHLL
                     GYLGEVDENVTAKNNYYQMGDYIGISGIEQSYEKELRGRRGTRYMMVDNFNREKGRFK
                     EGKYDTLAISGENLKLSLDAELQEYAELLFQNKRGSAVAIDPSTGEILAMVNSPTYNP
                     NLMVGRERSRNYGRLLKDEDKPLFNRAMMAYYPPGSTFKIINSLIGLQEKVITPETYF
                     SCAGGYRVGNIFVHCHPHSSPLDLRTAVAVSCNSYYCQTFRGVIDNRKYNRTEDAFDA
                     WRNYVLSFGIGKKIDVDMPHELRGNVPTVDYYNRFFGKGRWKSSTIISLAIGQGELGI
                     TPLQMANAACIVANKG"
     gene            complement(1409..>1588)
                     /locus_tag="PK134_08665"
     CDS             complement(1409..>1588)
                     /locus_tag="PK134_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRB38961.1"
                     /translation="MVYYLFISIFLHHLFLFYTEVFRFNDFGATFLRVCLSTITSVVI
                     IFIILLLFDKTKVQR"
BASE COUNT          491 a          312 c          267 g          518 t
ORIGIN      
        1 cctttattgg ctacaataca agccgcatta gccatctgta aaggggtaat tcctaattca
       61 ccttgtccta ttgctaatga aattattgtt gatgatttcc atcgtccttt tccaaagaaa
      121 cgattgtagt aatcaactgt tggaacgttg cctcgcaatt catgcggcat atctacatca
      181 atttttttac caataccaaa gcttagaaca taatttcgcc atgcatcaaa agcatcttct
      241 gttctgttgt attttctatt gtcaatcacc cctcggaaag tctggcagta ataagagttg
      301 cacgatactg caactgcagt acgtaaatca agaggagagc tgtgcggatg acaatgaaca
      361 aaaatatttc caaccctata accacctgca caactgaaat atgtttcagg tgtgatgacc
      421 ttctcttgca aaccaatgag tgagttgata attttaaatg ttgaaccggg aggataatag
      481 gccatcatgg cacgattaaa aagtggctta tcctcatctt ttaataaacg accatagttg
      541 cgcgaacgct ctctacctac catcaggtta ggattgtagg tagggctgtt taccatagca
      601 agtatttcac ctgttgaagg gtcaatggca acggcacttc ctcttttatt ctgaaacaat
      661 agctctgcgt actcttgtaa ttctgcatct aaggaaagtt ttaaattttc accggatatg
      721 gcaagtgtat catatttccc ttctttaaaa cgtcctttct cacgattaaa attatctacc
      781 atcatatatc gtgtaccacg tctgcctctt aattcttttt cataactctg ctcaatacca
      841 ctgatgccta tataatcacc catctggtaa tagttatttt ttgcagtcac attttcatct
      901 acctcaccaa gataacccag caagtgtgct gcagtgttgt aaggatattt tctgatggta
      961 cgcggttgaa cactgaagcc tttatactta aaaagttttt cctgaagtgt tccgtatgtt
     1021 tctgctgaaa tctgtttttc aaaaattgat gaacgataag gagaatagtt ttttgctttc
     1081 tgaaatttct ttctgaaatc ttcaatactg atttttatga tggaacaaaa ttctgctgtg
     1141 tccatttctt tgacttcacg gggtgttatc attaagtcat aaactgtctg attaaacacc
     1201 aacaactttc cgtttctgtc ataaaccaat cctcttgaag ggtagtcaat tattctgcga
     1261 agaacgtttt catctgccga caacttatat ttatcttcaa ttacctgtaa atagaacaaa
     1321 cgcccgatat aggtaatagc taccaatgca atagtaatga gtaaaacgag gcgttgtgtg
     1381 ctatgcgaag ttttcaaaat tcagaatttt agcgttgcac ttttgtttta tcgaacaata
     1441 ataaaatgat aaaaataatt acaacagatg taatggttga taaacatact ctgaggaatg
     1501 ttgctccgaa atcgttaaaa cggaacactt ctgtatagaa caaaaacaga tgatgcagaa
     1561 aaatactgat aaataaataa tacaccat
//
LOCUS       DAORQT010000333         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16904_length_1587_cov_4.622715, whole genome shotgun sequence.
ACCESSION   DAORQT010000333 DAORQT010000000
VERSION     DAORQT010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16904_length_1587_cov_4.622715"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PK134_08670"
     CDS             complement(<1..738)
                     /locus_tag="PK134_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB38962.1"
                     /translation="MLEKIISFSIKNKLIIAMMTLALIIWGVWSAGRLPIDAVPDITN
                     NQVQIITLCPTLAGQEVEQLVTYPIEQSIANLPDLEELRSISRFGLSLITVVFKENVD
                     VYFARQLINERLNEAVENIPAGVGKPEMGPVSTGLGEVYQYIIHPKKESENKYSAMDL
                     RTMQDWIVARQLYGTPGIAEVNSFGGKLKQYEVAVNPSRLLAMGVTIPDIFSALQKNN
                     ENTGGAYIDKKPNAYFIRGIGLIGSFDD"
     assembly_gap    903..933
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<934..1125)
                     /locus_tag="PK134_08675"
     CDS             complement(<934..1125)
                     /locus_tag="PK134_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38963.1"
                     /translation="MPCTDNDALDIFSDLRHSSQSTSANDDHNHEAETCSPFCICACC
                     GHTAATQFTFLYFTTCTAVS"
     gene            1370..>1587
                     /locus_tag="PK134_08680"
     CDS             1370..>1587
                     /locus_tag="PK134_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRB38964.1"
                     /translation="MNHKLRLFLTAFALFFTTMLHADEGMWLLSTLKQLNEADMQKMG
                     FKLTADDIYNINKSSMKDGIIHFGGGCTG"
BASE COUNT          506 a          301 c          261 g          488 t
ORIGIN      
        1 atcatcaaac gaacctatta aaccaatacc ccgaataaaa taggcgtttg gttttttatc
       61 aatatatgcc cctcctgtgt tctcattatt cttttggagt gccgaaaaaa tatccggtat
      121 ggtaacaccc attgcaagta acctcgaagg atttacagct acttcatact gttttagttt
      181 tcctccaaaa gaatttactt cggcaatgcc cggtgtgccg tatagctgtc gtgcaacaat
      241 ccaatcctgc atggtgcgca agtccattgc tgaatatttg ttttcgcttt ctttttttgg
      301 atgaataatg tactgataca cttctcccaa tcctgtactt acaggaccca tctcaggctt
      361 gcctacacct gcaggtatat tctcaacagc ctcattcaat ctttcattga tgagctgtcg
      421 tgcaaaatac acatctacat tttctttaaa aactactgta atcaacgaaa gaccaaagcg
      481 cgaaatactt cgcaactctt caaggtcagg aaggtttgca atactttgtt ctatcggata
      541 agtcaccaac tgctctacct cctgtccggc aagtgtaggg cataatgtaa ttatctgtac
      601 ctgattgttg gtaatatcag gaacagcatc tattggtaat ctgccagcac tccacacacc
      661 ccatattatg agtgccagcg tcatcatggc aatgattaat ttgttcttta tactgaacga
      721 aataattttt tctaacatat ataatcagtt aaaattaaac atataactta ttgtatgcca
      781 ttttgcaata gcattaattc acattgcaaa aagcaatgtc ataaatttat attaactgat
      841 tttgggcggc tgccagattc ctatataact ctctgtagaa agtaaaattt tatagggatt
      901 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacttact gcagtgcagg tggtgaaata
      961 taaaaacgta aattgtgtag cagccgtatg accacaacat gcacaaatac aaaatggtga
     1021 gcaggtttct gcttcatgat tatgatcatc atttgcagaa gttgattgtg atgaatgacg
     1081 caaatctgaa aaaatatcta atgcatcgtt atctgtacat ggcattacag ataaagctaa
     1141 aaagtatata ctgaatatgt aggctgctat tttcaacagg tcaaatttac gaaaaaccca
     1201 atgaatggtt actgaagaaa acaactgatt tgtgtcataa aatgtatgcg aaaattttgg
     1261 aactaacaga ataaatttgt taccgtctaa tattcctctg tcattaacaa gtttttaacg
     1321 attataagaa taaaaataca gatattcgcc acacaaaatc ttttaaatta tgaatcataa
     1381 attacgactg tttctaacag cctttgcact gttttttact acaatgttgc atgccgatga
     1441 aggtatgtgg ctgctgtcaa ctttaaaaca acttaatgag gcagacatgc aaaaaatggg
     1501 cttcaagctc acggcagatg acatttacaa catcaacaaa tcatccatga aagatggtat
     1561 cattcacttt ggtggtggat gtacagg
//
LOCUS       DAORQT010000334         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_16985_length_1582_cov_5.128356, whole genome shotgun sequence.
ACCESSION   DAORQT010000334 DAORQT010000000
VERSION     DAORQT010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16985_length_1582_cov_5.128356"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="PK134_08685"
     CDS             complement(<1..1574)
                     /locus_tag="PK134_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRB38965.1"
                     /translation="MPNLRSVVLKNLYPTWEKTSDVARTLNYSFIVRDNDVNGGQTAS
                     DLMQVTVASAGPFVVTSQNTPTNWNAGASETVTWNVAGTDIAPVNTPFVNIFLSLDSG
                     FTYPYTLATNVPNNGSASIVVPAGAASQWGRVMVRGAGNIFYALNGSYINIQASEFVM
                     NMSSTAQSVCPPNDITYNFMYNTFLAFNETTVFSITGLPAGATGTFTPATAVNDSTPV
                     QLLISGLTPSMTGTYNLTITGTSPSVTKTTSVTLDILDVNLPAATLTLPADGSSGIAS
                     IATLTWQTAASPGTLYDIEVATDSLFTNVIASATGISTNTFTTTPLLSSTTYYWHVKS
                     YNSCFANSYSAAWSFTTNNCSNLASANVPVVIPSSGAPTVTSTLNIASAGTITDVNVV
                     GLNGTHTWISDLTISIKSPQNTTVTLFDQICTNENDFDCNFDDAAAPGALPCPPIGGG
                     TYNAVTPLSVFNGQQAAGNWTLTVTDHFNSDGGSLNGWGLEICTTTPTGIETHIVSTS
                     MILYPNPVKELLNVNVA"
BASE COUNT          464 a          294 c          347 g          477 t
ORIGIN      
        1 gcaacattta cgttcaacaa ttcttttaca ggattaggat ataaaatcat ggaagtagaa
       61 actatgtgag tttcaattcc tgttggcgta gtggtgcaga tttcaagtcc ccagccattc
      121 aatgagccac catcagaatt aaaatgatcg gtaactgtta gtgtccagtt gcctgctgct
      181 tgctgtccgt tgaaaacact cagtggtgta acggcattgt aagtgccgcc accaattgga
      241 gggcaaggta atgcacccgg tgcagctgca tcatcaaaat tacagtcaaa atcattttca
      301 ttggtacaaa tctgatcaaa caatgttaca gttgtgtttt gcggactttt aatgcttatg
      361 gtaaggtcgc taatccaggt gtgtgtgcca ttaagaccaa caacattaac atctgttatt
      421 gttccagcag atgctatgtt aagtgttgaa gtaactgttg gtgcaccact tgatggaata
      481 acaacaggaa cattggcact tgctaaattc gagcaattgt tggttgtgaa agaccatgca
      541 gcgctgtagc tatttgcaaa acatgaatta taagacttta catgccaata ataggttgta
      601 cttgataaca atggagtagt ggtaaaagta tttgtactta tgccggtagc agatgcaata
      661 acattcgtaa ataatgaatc tgttgccact tcaatatcat atagtgttcc cggacttgca
      721 gcagtctgcc aggttaatgt tgcaatagat gcgatgccac tgcttccatc agcaggtagt
      781 gttaaagttg ctgcaggtaa gtttacatct aaaatatcaa gtgtaacaga agtagtcttg
      841 gttacggatg gtgatgtacc agtgatagta agattataag ttccggtcat ggaaggtgtt
      901 aatcctgaaa tcagcaattg cacgggagta ctgtcattta ctgctgtggc aggagtaaaa
      961 gttcctgttg caccggcagg aagccctgtt atagaaaaca cagttgtttc attgaatgcc
     1021 agaaatgtgt tgtacataaa attataagta atgtcgtttg gagggcaaac actttgtgca
     1081 gtactgctca tgttcattac aaattctgaa gcctgaatgt tgatatagct gccatttaat
     1141 gcatagaaaa tattacctgc accacgtacc atcacacgtc cccattgtga tgctgcacct
     1201 gccggaacaa ctatgctagc acttccgtta tttggtacgt tggttgcaag agtatatggg
     1261 taagtaaatc cactgtcgag tgaaagaaaa atatttacga atggcgtatt cacaggagca
     1321 atatctgtgc ctgccacatt ccatgttaca gtttctgaag cacctgcatt ccagttagta
     1381 ggtgtgtttt gtgaagtaac tacaaatgga cctgcagaag caacagttac ttgcataagg
     1441 tctgatgccg tttgaccacc attcacatcg ttatctctaa caataaatga atagtttaat
     1501 gttctggcta catcggatgt tttttcccag gtaggatata ggttttttag cactacactt
     1561 ctcaggttgg gcatataacg gt
//
LOCUS       DAORQT010000335         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17035_length_1578_cov_5.973079, whole genome shotgun sequence.
ACCESSION   DAORQT010000335 DAORQT010000000
VERSION     DAORQT010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17035_length_1578_cov_5.973079"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PK134_08690"
     CDS             <1..991
                     /locus_tag="PK134_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRB38966.1"
                     /translation="DFQKSSDLFTSLITKYGFKLLDDFDAVHDENNQQNSEVVFAVQY
                     TTDMITNATNVGGEGGGGNNLHLFFGMQYDTQAGMVRDVFNGRPFKRLRPTAFCLNNV
                     FGERVNDSRYKKTFKDTWRCNSPGSFNTSFDNSKARVTFAAGDTTIFIPGFEMSQAER
                     AKRKYQVLVPSKYDEALFPTLQKFFDTKRSDRTEPRGSRDYFVWRLADIYLMRAEANL
                     QLGKKAEATADVNVVRERAAFPGKKDAMKITESQLTFDFLVDERARELAGEQMRWLDL
                     KRWGLLIDRVKKYNPQAQAVAEKHYLRPIPQTQIDRSAKTAENTSVFAQNPGY"
     gene            complement(1095..>1578)
                     /gene="rsgA"
                     /locus_tag="PK134_08695"
     CDS             complement(1095..>1578)
                     /gene="rsgA"
                     /locus_tag="PK134_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRB38967.1"
                     /translation="YNGMGYTCFTTSSVTGEGVETFQQLLQGKVSVLSGHSGVGKSTL
                     VNTIAPDLHLRTNEVSTFANKGVHTTTFAEMFELAPDTFIIDSPGIKELGLSEMEKEE
                     IGHYFPEIRDLIGQCRFYNCLHIDEPKCAVKEAVEAGTIAMSRFESYLSMVGGEDNRR
                     "
BASE COUNT          459 a          317 c          358 g          444 t
ORIGIN      
        1 tgatttccaa aaatcatctg atttgtttac gagccttatt acaaagtatg gttttaaact
       61 tttagatgat tttgacgctg tccacgacga aaataaccaa cagaattcag aagtggtttt
      121 tgccgtacaa tacaccacag acatgattac caacgctacc aatgtaggtg gagaaggtgg
      181 tggtggaaat aaccttcatt tgtttttcgg tatgcagtat gatactcaag caggtatggt
      241 tagggatgta ttcaatggtc gtccgtttaa aagattgaga ccaacagctt tctgtttgaa
      301 taatgttttt ggtgaaagag taaatgattc tcgttacaaa aaaacgttta aagatacttg
      361 gagatgtaat agcccgggtt ccttcaatac ctcgttcgat aactcaaagg ctcgagttac
      421 ttttgccgcc ggtgatacta caattttcat tcccggtttt gaaatgagcc aagcagaaag
      481 agctaaaaga aagtatcagg tattggttcc aagtaagtat gatgaggctc tgtttccaac
      541 gttgcagaag ttttttgata ccaaacgttc agacagaact gagccaagag gttcaagaga
      601 ttattttgta tggagattag cagatattta tctcatgcgt gccgaagcta atttgcaact
      661 tggtaaaaaa gcagaagcta ctgctgatgt aaatgtagta agagaaagag ctgctttccc
      721 agggaaaaaa gacgcaatga aaatcactga gtctcaattg acatttgatt tccttgttga
      781 tgagcgtgct cgtgaattag cgggagaaca gatgcgttgg ttggatttga aacgttgggg
      841 gcttttaatt gatagagtta aaaagtataa cccacaggct caagcagtgg cagagaagca
      901 ctatctgcgc ccgattccgc aaacccaaat tgacagaagt gctaaaacgg cagagaatac
      961 ttcggtgttt gctcaaaacc ccgggtacta atcagtgtac agtttaaagc agaatacaaa
     1021 aagagagaag ctttcgcttc tctctttttt gttgccaggt taagggcaga ggcaagccct
     1081 gcccttacgg tcaactacct tcggttatct tcgccgccta ccatgctcag gtagctttca
     1141 aatcgactca tggcgattgt tccggcttct acggcctctt tgacggcgca tttgggctcg
     1201 tcgatgtgga ggcaattata gaaacggcat tgacctatca ggtcgcgtat ctcaggaaaa
     1261 taatgaccga tttcttcctt ttccatttcc gataatccca attccttaat gcccggcgaa
     1321 tcaatgataa aggtatcggg ggccaattca aacatttcgg caaaggtggt cgtgtgtaca
     1381 cctttgttgg caaaagtaga gacttcattg gtacgcagat gcaggtccgg tgcaatggta
     1441 ttgaccaatg ttgattttcc cacgcccgaa tgccccgata acaccgaaac tttgccctgc
     1501 aacaactgct gaaatgtttc aacgccctca cctgtcacgc tggaggtggt aaaacaggta
     1561 taacccattc cattatag
//
LOCUS       DAORQT010000336         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17283_length_1564_cov_5.301524, whole genome shotgun sequence.
ACCESSION   DAORQT010000336 DAORQT010000000
VERSION     DAORQT010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17283_length_1564_cov_5.301524"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK134_08700"
     CDS             <1..274
                     /locus_tag="PK134_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB38968.1"
                     /translation="TPDNDGKNDLFVPFPYRYVDHVELTIYNRWGNKVFETHDKDILW
                     KGTKDNGSTRLSDGVYYYIGKVYEIFIDGIKPRTLKGAVNLIGGAK"
     gene            274..870
                     /locus_tag="PK134_08705"
     CDS             274..870
                     /locus_tag="PK134_08705"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRB38969.1"
                     /translation="MFTGIIETLGIVKKIENEGSNRHFTVSCSFADELKIDQSVAHNG
                     VCLTVVNINHENKTYKVTAVDETLRKTNLSSLVEGNEVNLERGMLLNTRLDGHIVQGH
                     VDGTAVCTSITDENGSWKFIFENDGSARNLIVEKGSVTVNGVSLTVVDAGDYMFSVAI
                     IPFTMQHTTFKNLKRGQTVNVEFDIIGKYVARLTEKSV"
     gene            complement(862..>1564)
                     /locus_tag="PK134_08710"
     CDS             complement(862..>1564)
                     /locus_tag="PK134_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRB38970.1"
                     /translation="AYPAVDWISFADDFVNSLGLTRTTFTTQIEPYDNLSALFDNLKR
                     INNILLDLCRDVWSYISMNYFSQTVKAGEIGSSAMPHKVNPIDFENAEGNLGIANALF
                     EHLSAKLPISRLQRDLTDSTVLRNTGVPFAHTLIALKSLLKGLNKISPNTQVISADLN
                     RHAVVIAEGIQTILRREGYPNPYEALKDLTRGKENITLQDIHQFINTLQVGDTVKAEL
                     LKITPENYTGVNWSD"
BASE COUNT          507 a          250 c          322 g          485 t
ORIGIN      
        1 tacacctgac aatgatggaa agaacgattt gtttgttcca tttccttatc gttatgttga
       61 ccatgttgaa ctcaccatct acaaccgttg gggaaataaa gtttttgaaa cacatgacaa
      121 ggatatttta tggaaaggaa caaaagataa tggctcaaca agactgagtg atggggtata
      181 ttattacatt ggaaaggtgt atgaaatttt cattgatgga ataaaaccac gtaccctcaa
      241 aggtgctgtt aacctgattg gaggtgctaa ataatgttta caggaattat tgaaacttta
      301 ggcattgtaa agaaaataga aaatgaaggc agtaatcgtc attttactgt ttcatgttct
      361 tttgctgatg aattaaaaat agatcagagt gttgcacaca atggtgtttg cttaactgtt
      421 gtaaatatta atcatgaaaa caaaacctat aaggttactg ctgttgatga aacattgcgc
      481 aaaacaaacc ttagtagttt ggttgaaggc aatgaagtaa atcttgaaag aggcatgttg
      541 ttgaatacac gtttagacgg acatattgta caggggcatg ttgacggtac tgctgtttgc
      601 acttctataa cagatgaaaa tggaagctgg aaatttattt ttgaaaatga tggctcagca
      661 cgaaatttaa tagttgagaa aggctctgta actgtcaacg gtgtaagcct tacagtagtt
      721 gatgccggtg attatatgtt ttcagttgca ataattccat tcaccatgca gcataccaca
      781 tttaaaaatc ttaaacgcgg acaaaccgta aatgttgagt ttgatattat cggaaaatat
      841 gttgcaaggc tgacggaaaa atcagtctga ccaatttaca ccggtataat tttcaggagt
      901 aattttcagc aattcagctt ttactgtatc accaacctga agtgtgttga tgaactgatg
      961 aatatcctgc aaagtgatat tctcttttcc tcgtgttaaa tcttttaatg cttcataagg
     1021 attagggtag ccttcgcgtc tgagaattgt ctgtatgcct tctgcaatta ctactgcatg
     1081 acgatttagg tcagcagaaa ttacctgagt gtttggcgaa attttattta atcctttcag
     1141 taaagatttc agtgcaatga gtgtatgtgc aaacggcaca ccggtatttc tcaacacagt
     1201 gctatcagtc aaatcacgtt gaagtcttga aataggaagt ttggctgaca gatgttcgaa
     1261 aagtgcattg gcaatgccaa gattaccttc tgcattttca aagtctatgg gattaacttt
     1321 atgtggcatt gcagaagaac caatctcacc ggctttgaca gtttgcgaaa aataattcat
     1381 gctgatgtaa ctccaaacat cacgacataa atccaataaa atattattga tgcgttttag
     1441 attatcgaat aaagccgaaa ggttgtcata aggttcaatc tgtgttgtga atgttgtgcg
     1501 tgttaatccg agtgagttca caaaatcatc agcaaaagaa atccagtcaa cagcagggta
     1561 agcc
//
LOCUS       DAORQT010000337         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17376_length_1558_cov_7.649368, whole genome shotgun sequence.
ACCESSION   DAORQT010000337 DAORQT010000000
VERSION     DAORQT010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17376_length_1558_cov_7.649368"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="rpsU"
                     /locus_tag="PK134_08715"
     CDS             complement(<1..188)
                     /gene="rpsU"
                     /locus_tag="PK134_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRB38971.1"
                     /translation="MIIVQIKDNEPLDKALKKFKKKFEKTGVVKQLRSRQAFEKPSVA
                     RRTEVKKAAHGKTIVLKN"
     gene            complement(364..>1558)
                     /gene="purD"
                     /locus_tag="PK134_08720"
     CDS             complement(364..>1558)
                     /gene="purD"
                     /locus_tag="PK134_08720"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRB38972.1"
                     /translation="LYIMPGNAGTLQLGTNISGSVNDFDHIKKTVLEKKIDMVIVGPE
                     EPLVNGIHDFFLNDSQLKSVPVIGPESSGARLEGSKDFSKAFMLRNNIPTARYETFES
                     SQLQQGYDFLKTLSAPYVLKADGLAAGKGVIICETETEAREQLHHLLINQPFGKAGNK
                     VVIEEFLKGIELSVFVLTDGNNYCLLPEAKDYKRIGDNDTGLNTGGMGSVSPVPFADA
                     AFMQKVEDKIIRPTINGLKHENISYTGFLFIGLMNVNSNPFVIEYNCRMGDPETEAVF
                     PRIKTDFIELMNSVANKKLHEVQLKTDDRTSFTVMMVSGGYPETYEKGKIISGLEKVK
                     GVNVFHAGTIIHDGKVLTNGGRVLAVNALGKNLAEAREKVYGQLTEITYEKAFYRKDI
                     GKDLM"
BASE COUNT          443 a          321 c          262 g          532 t
ORIGIN      
        1 tcattcttga ggacgattgt cttcccgtga gcagcttttt ttacttctgt tctgcgggct
       61 actgatggtt tttcaaatgc ctgacgtgaa cgcagttgtt ttacaacacc tgttttttca
      121 aacttctttt taaacttctt taaagcctta tccaaaggct cattgtcttt aatttgaaca
      181 ataatcatat tataaataca aaatgttttg ggagcgcaaa ggtaagtaaa taattggcat
      241 aataatcaat acaatagaaa atttttaaga aagtactcac ctgcctgtat ttgaccttaa
      301 ctattgatta ttaatatgat ggagtatatt tcttttctgt ttttatgaca aagcaggtgg
      361 aaattacatc aaatccttgc caatatcctt cctgtaaaag gctttttcgt aggtaatttc
      421 tgtcaattgc ccatagactt tttcgcgggc ttctgccaga tttttaccca atgcatttac
      481 agcaagtaca cgtccgccat tggttagtac tttgccatca tgtatgatgg tgcctgcatg
      541 aaacacgttt acccctttaa ctttttcaag ccctgaaata atttttcctt tttcataagt
      601 ttctgggtat ccacccgaaa ccatcataac agtaaacgat gttctgtcat ctgtttttag
      661 ctgaacctcg tgaagttttt tatttgctac actattcatc aactcaataa aatcagtttt
      721 tattctcgga aaaacggctt ctgtttccgg gtcgcccatg cggcagttgt attcaataac
      781 aaagggattg ctattaacat tcatcagtcc gataaataaa aatccggtgt atgaaatgtt
      841 ttcatgcttt aatccattta tggttggtct gataatttta tcttctactt tttgcataaa
      901 agcagcatct gcaaacggaa ccggtgatac tgatcccatt ccacctgtat tgagtccggt
      961 gtcattgtcg ccaatgcgtt tataatcttt tgcttccggc agcagacaat aattgttgcc
     1021 atcagtcaaa acaaaaacag agagttcaat acctttcaga aattcttcta tcactacttt
     1081 gtttcctgcc tttccaaaag gttgattgat gagaagatga tgcaattgct ctcttgcctc
     1141 agtttctgtt tcgcaaataa taacaccttt gccggcagcc aaaccatcag cctttagtac
     1201 gtagggtgca gatagtgttt tcagaaaatc ataaccctgc tgcagttgcg atgattcaaa
     1261 agtttcataa cgtgctgtag gaatattatt ccgcaacata aaagctttag aaaaatcttt
     1321 actgccttca agtctggcac cggaactttc cggaccaata acaggaacag attttaactg
     1381 actgtcatta agaaaaaaat catggatgcc attaaccaaa ggctcttcgg ggcccacaat
     1441 taccatatca attttctttt caagcacagt ttttttgatg tggtcgaagt cattcacgct
     1501 gcctgaaata tttgttccca actgtaatgt gcctgcatta cccggcataa tatataag
//
LOCUS       DAORQT010000338         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17455_length_1554_cov_4.070714, whole genome shotgun sequence.
ACCESSION   DAORQT010000338 DAORQT010000000
VERSION     DAORQT010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17455_length_1554_cov_4.070714"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK134_08725"
     CDS             <1..370
                     /locus_tag="PK134_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRB38973.1"
                     /translation="QIAAFLRAHPKIEKVYWPGFEDHPNHHIAKMQMRDFGGMISFDL
                     KSHSQEEAFKLASSVKVFSLAESLGGVESLVNHPASMTHASVPVAERVRVGVTDSLLR
                     LSVGVEDIDDLIADLKQAIG"
     gene            455..>1554
                     /locus_tag="PK134_08730"
     CDS             455..>1554
                     /locus_tag="PK134_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRB38974.1"
                     /translation="MLKKSTNFLLLMMAMIATITVNAQNTHWCATDEVTNAALQNSAA
                     ARAAQNQLEAFTQQYVQNFQPSYFRSTGTAVYVIPVVFHVIHNWGPENISDAQVYDAI
                     RVLNRDYNGQRTDTANIIPDFKALNADVQIEFRLAHKDPNGNCTNGIDRQQSLLTYDA
                     GDQSKLNPWPYNKYLNIWTISNFGSSHAQAAAYAYKPGTAPPGKDGIIALHNYVGSIG
                     TGSSTGQYTLTHEIGHWLNLNHTWGGTNQPGVACGDDNVTDTPVTKGYTTCPSASNAD
                     VCNPGVIENYQNYMDYSYCYYMFTLGQKARMWAALNSGAGLRNQVHTATNLTNTGTDG
                     GTYVCAPTASFSPVQKVICEGGTVSFSDASLNT"
BASE COUNT          488 a          289 c          322 g          455 t
ORIGIN      
        1 acaaatagca gcatttcttc gtgctcatcc aaaaatcgaa aaagtttatt ggccgggctt
       61 tgaagatcat ccaaaccatc acattgcaaa aatgcagatg cgcgattttg gtggtatgat
      121 ttcttttgac ttaaaaagtc actctcagga agaggctttt aagttagcgt catcggtaaa
      181 agtgttttca ttggctgaat cattgggtgg agtagagtct ttggtaaacc atcctgcatc
      241 aatgacacat gccagtgttc cggttgcgga aagagtaaga gttggtgtaa ccgactcatt
      301 attgagatta agtgtgggtg ttgaagatat agacgattta attgcagatt tgaaacaagc
      361 aatagggtag ttccttcaca aaaaatttac attgaacata tgctcaaaac acacgatttt
      421 attacttttg attcattata aataaattaa aacaatgcta aaaaaatcta ctaatttttt
      481 attactgatg atggccatga ttgcaaccat tacagtcaat gcacagaaca cacattggtg
      541 tgctactgat gaagttacta atgctgcact gcagaatagt gctgctgccc gtgctgcaca
      601 aaatcagtta gaagcgttta cgcaacagta cgtacaaaac tttcaaccaa gctatttcag
      661 aagtactggc actgctgtgt atgttatccc tgtggtattt catgtcattc ataattgggg
      721 tcctgagaat atttcagacg cgcaggttta tgatgcaatc agagttttga accgcgatta
      781 taacggacag cgaacagata cagcaaacat catccctgat tttaaagcat tgaatgccga
      841 tgttcagatt gaattccgtc ttgcacataa agatcctaat ggaaattgta ctaatggtat
      901 tgacagacaa caatcgttgt taacttatga tgccggtgat caatcaaagt tgaatccatg
      961 gccttacaat aaatatttaa atatctggac tatttctaac ttcggttcat cgcatgcaca
     1021 agcggcagct tatgcataca aacccggtac agcgcctccc ggtaaagatg gaatcattgc
     1081 attgcataac tacgttggta gcatagggac tggtagttca acaggtcaat atacacttac
     1141 gcatgaaatt ggtcattggt taaatctgaa tcatacatgg ggtggaacca atcaacccgg
     1201 tgtagcttgc ggtgatgata acgtaacaga tactccggta actaaaggtt atactacatg
     1261 tccatccgct tcaaatgcag atgtgtgtaa cccgggtgtt atagaaaact atcaaaacta
     1321 catggactat agctattgct attacatgtt tactttagga cagaaagcgc gtatgtgggc
     1381 tgcattaaat tcaggtgcag gcttgcgtaa tcaggttcat actgcaacca atttaactaa
     1441 tacgggaacc gatggaggta cgtatgtttg tgctcctaca gcaagttttt cacccgtaca
     1501 aaaagtaatt tgcgaaggcg gaactgttag ctttagtgat gcaagtttaa atac
//
LOCUS       DAORQT010000339         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17476_length_1552_cov_5.076820, whole genome shotgun sequence.
ACCESSION   DAORQT010000339 DAORQT010000000
VERSION     DAORQT010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17476_length_1552_cov_5.076820"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PK134_08735"
     CDS             complement(<1..359)
                     /locus_tag="PK134_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB38975.1"
                     /translation="MTIKRFFLAAFCMLAVSLVNAQTATIKGFVYAAENGEPVPFASV
                     FLKGTTFASFTDINGYYSMVKIPPGKYRVMATTLGYDTAFADVDLVADEMENRKLVMK
                     KKNVELKQVEVSAERQT"
     gene            complement(365..769)
                     /locus_tag="PK134_08740"
     CDS             complement(365..769)
                     /locus_tag="PK134_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRB38976.1"
                     /translation="MQFWLVKSEPFKYSWEQFVADGKTFWDGVRNNAARLHLLAMKKG
                     DQVLFYHSNEGLAVMGIAKVEKTAYTDPTADDTRWVGVELTPFKALKKPVTLAQVKDD
                     ARLKDIALVRIGRLSVMPLKKEEFNVILELSK"
     gene            complement(820..1407)
                     /gene="purN"
                     /locus_tag="PK134_08745"
     CDS             complement(820..1407)
                     /gene="purN"
                     /locus_tag="PK134_08745"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRB38977.1"
                     /translation="MNKKRIAILASGSGTNAENIAAYFKNNEYAEVALILTNVATAGV
                     IVRAQKLNIPCLFMDKKTVSDGRLMLDLLKQHSIDLVVLAGYLKLMPAEVVSTFQHRM
                     INIHPALLPLFGGKGMYGHNVHEAVLQAGVAETGITIHYVNSRFDEGEIIFQKSMPVL
                     PDDTAERIASRIHRLEYEHYPSVIEHLAKGLFKDN"
BASE COUNT          457 a          344 c          273 g          478 t
ORIGIN      
        1 tttgtttgtc tctctgccga tacctcaacc tgtttcaact ccacattttt tttcttcatc
       61 actaatttac ggttttccat ttcatcggca acaagatcaa catctgcaaa agcagtgtcg
      121 tagcctaatg ttgttgccat tacgcggtat tttcctggag gaattttcac catggaataa
      181 taaccgttaa tgtctgtaaa agaggcaaag gttgttccct tcagaaaaac acttgcaaag
      241 ggaacaggct caccattttc tgcagcataa acaaaccctt tgatggtggc tgtttgtgca
      301 tttaccagag aaacggcaag catacagaaa gcagctaaaa aaaatctttt tatcgtcata
      361 aaaactattt acttaattct aaaattacat taaactcttc ttttttaagt ggcattaccg
      421 acaggcggcc tatgcgcacc aatgcaatat ctttcaaccg tgcatcatct tttacctgtg
      481 ccaatgttac aggcttcttc aatgctttga atggtgtcaa ctccacacct acccaacgtg
      541 tatcgtcagc agtgggatcg gtatatgctg tcttttcaac tttagcaata cccataacgg
      601 caaggccttc attgctgtga taaaacaaaa cctgatcgcc ttttttcatg gcaagtaaat
      661 gcagccgtgc agcgttgttg cgcacaccgt cccaaaatgt ttttccatcg gcaacaaact
      721 gctcccacga atatttaaac ggctctgatt ttaccagcca aaactgcata ctatttttaa
      781 agtcggacaa agaaataaaa ataatcgaat acggatttgt taattatcct taaataaacc
      841 ctttgccaaa tgctcaatta cggatgggta atgctcatat tcaagacgat gaatacgtga
      901 cgctatacgc tctgccgtgt cgtcaggtag cacaggcata cttttctgaa aaataatttc
      961 accctcgtca aagcggctgt ttacgtagtg aatggttatg cccgtttctg caacacctgc
     1021 ctgcaacact gcctcatgaa cgttgtgacc atacatgcct ttgcctccga aaagtggcag
     1081 caatgcagga tgaatattta tcatgcgatg ctgaaaagtt gaaacaactt cggcaggcat
     1141 cagctttagg tatccggcaa gcacaaccaa atcaatgcta tgttgcttta acaaatcaag
     1201 catcagacgg ccatcgctaa cagttttttt gtccataaaa agacaaggaa tgtttaactt
     1261 ctgtgcgcgt actatcacac ctgccgttgc cacattggtg agaatcaatg ccacttctgc
     1321 atattcattg ttcttaaaat aagcagcaat attttcggca ttggtgcccg accccgatgc
     1381 taatatggct attcgctttt tgttcattgc acaattatga gcgtgtaaaa atagcattct
     1441 gtaaatgata ggcttagata tttttttggc attattacaa tcattcattt ggttattata
     1501 tagcctgtat attaaatttg tagtaaattc attaaaccac ataacatgta tt
//
LOCUS       DAORQT010000340         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17543_length_1548_cov_3.578701, whole genome shotgun sequence.
ACCESSION   DAORQT010000340 DAORQT010000000
VERSION     DAORQT010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17543_length_1548_cov_3.578701"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK134_08750"
     CDS             <1..402
                     /locus_tag="PK134_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRB38978.1"
                     /translation="MSMANNLEVRSPFLDYRVVDFAFSLTDKYKIDNKAQKKIVKDAF
                     RSLLPEEIYHRGKRGFEVPLIKWFQTELKDKIDLLLGEEMIQSQALFNPNTIKQLRHA
                     VMNNPSGESQAKMWALIVFQNWWLTHHLKSN"
     gene            405..>1548
                     /locus_tag="PK134_08755"
     CDS             405..>1548
                     /locus_tag="PK134_08755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB38979.1"
                     /translation="MPRVLRIINRLNLGGITYNVTYLTRFMQPEFETMLVSGVKTEDE
                     ESSEFILDQYEIKPHYIREMKREINFSSDRKAYAELKELIKKFKPDIVHTHAAKAGTL
                     GRLAAFSCNVPVVVHTFHGHVFHSYFSPLKTKLFIEIERYLAKKSSAIVTISELQKHE
                     ICNVFKVSAPEKTHIIPLGFDLSRFRENTDEKRKAFRETYKITDDVIAIGIIGRFAAI
                     KNHSFFLEAMKSVVSKTSKKVVAVLIGDGEEHESILDCCRKLNMPFAKDASESETKVV
                     LTSWIKDIDRALAGLDIVALTSHNEGTPVSLIEAQAAGKAVISTRVGGVENVVAEGHT
                     GFLCNKGDLQSFVNQLLQLVEDETLRNKMADNGWEHVRNKFHYTRLV"
BASE COUNT          519 a          245 c          321 g          463 t
ORIGIN      
        1 atgagtatgg caaataatct ggaggtgcgt tcaccatttt tagattatag ggtagtggat
       61 tttgcatttt cgttgacaga caagtataag attgacaata aagcccaaaa gaagatagtt
      121 aaagatgctt tcagatcact cttgcccgaa gagatttacc atcgtggcaa aagaggtttt
      181 gaagtgcctt taattaaatg gtttcagaca gagctaaaag ataaaattga tttactgctg
      241 ggagaagaaa tgattcagag tcaagcctta tttaatccaa atactattaa acaattgcgt
      301 catgctgtta tgaataatcc atcaggtgaa tctcaggcaa agatgtgggc tctcatagtt
      361 tttcaaaatt ggtggctcac acatcaccta aaatccaact gattatgcca cgtgtacttc
      421 gcattataaa cagacttaat cttggtggca ttacctacaa tgttacttat ctcacacgtt
      481 ttatgcagcc tgaatttgaa actatgttag tttcaggagt aaaaaccgaa gacgaagaaa
      541 gttcagaatt tatattagat cagtatgaaa taaagcctca ttacatcagg gaaatgaagc
      601 gtgaaataaa cttttcatca gacagaaaag cctatgctga acttaaagaa ttaataaaga
      661 aatttaaacc ggatattgtt catacccatg ctgcaaaagc aggtacattg ggccgtttgg
      721 ctgcattttc atgcaatgtt cctgtggttg ttcatacttt tcatgggcat gtttttcatt
      781 cttatttcag ccctcttaaa acaaaattat ttattgaaat tgaacggtat cttgctaaaa
      841 aaagttcagc tattgttacc ataagcgaat tgcaaaagca tgaaatctgt aatgttttta
      901 aagtgagtgc acctgaaaaa acacatatca tacctttagg ctttgacctt agtaggttta
      961 gagaaaatac agatgaaaaa cgaaaagctt tcagggagac atataaaata actgatgatg
     1021 taatagctat aggcattatc ggccggtttg ctgcaataaa aaatcattca ttcttccttg
     1081 aagcaatgaa gtctgtagtc tctaagacct ccaaaaaagt ggttgcagtt ttgataggtg
     1141 acggtgagga acatgaatct attttagatt gttgtcgtaa actgaacatg ccatttgcaa
     1201 aagatgcctc agagtcggaa acaaaggttg tgctgacatc ctggataaag gatattgaca
     1261 gggctttagc cggtttagat atagtagcgt tgacttcgca taatgaagga actcctgtca
     1321 gtttgattga ggcacaggct gccgggaaag cagtgattag caccagagtt ggaggtgtag
     1381 aaaacgtagt tgccgaaggt catacaggat ttttatgtaa caaaggtgat ttgcaatcgt
     1441 ttgtaaatca actcttgcaa cttgtggaag atgagacatt aagaaataaa atggctgaca
     1501 atggttggga gcatgttaga aataaatttc attatacccg attggtta
//
LOCUS       DAORQT010000341         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17708_length_1539_cov_4.197439, whole genome shotgun sequence.
ACCESSION   DAORQT010000341 DAORQT010000000
VERSION     DAORQT010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17708_length_1539_cov_4.197439"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>724)
                     /gene="rffA"
                     /locus_tag="PK134_08760"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(<1..>724)
                     /gene="rffA"
                     /locus_tag="PK134_08760"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRB38980.1"
                     /translation="YIKEVFSSGKISGDGNFTKRCHQFLQQKYNFKKVLLTTSCTDAL
                     EACAILLEIKEGDEVIVPSYTFVSTVNAFVLRGAKIIFADSNPDTANIDVNKIESLIT
                     SRTKAIVPVHYAGVACDMDSINAIAAKHNLYVVEDAAQAIDSFYKGKPLGSLGHLSAF
                     SFHDTKNIISGEGGMLVINDERFEKRAEIIREKGTNRSAFFRGEVDKYGWVDVGSSFL
                     PSEVTAAMLLAQLESIDKIQTRR"
     assembly_gap    725..761
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(788..>1539)
                     /locus_tag="PK134_08765"
     CDS             complement(788..>1539)
                     /locus_tag="PK134_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB38981.1"
                     /translation="TTGATSYSWDFGDGTAIDPNESPSHVYSDTGSYFITLTASSIFG
                     CEVQMQKPFDVIVPYVDLWLKSLSYNINDNYLNLSALLSNAGNTTVNNFELQIQPEGK
                     GSFIENANETIVYGTDKTYVLHTSISVNPLKLPDYICVTIKNVNGSSDKNPDNNEKCI
                     SLMPENGSLTVSPNPIDDLLSIHLNLPVSSEIKLVLYDAAGKKLTTLYNGPSDAGLKI
                     FSFKLPYLSKGVYILDAVSESFNKKVKFIRQ"
BASE COUNT          457 a          276 c          253 g          516 t
ORIGIN      
        1 cgtcttgttt gaatcttatc tattgattct aattgtgcca acaacatggc tgctgtaact
       61 tcacttggca aaaaagatga acctacatca acccatccat atttatccac ttcacctcta
      121 aaaaatgcag agcggtttgt gcctttttca cggattattt ctgcacgttt ttcaaatcgt
      181 tcatcattga tgactaacat tccgccttcg ccactgataa tattttttgt gtcatgaaaa
      241 gaaaatgccg acaagtgacc taaactaccc aatggttttc ctttataaaa agaatctatt
      301 gcctgtgctg catcttcaac aacatataag ttgtgtttcg ctgcaatggc attaatgcta
      361 tccatgtcgc aggctacacc ggcatagtgt acaggcacaa ttgctttggt gcgtgatgta
      421 atcagagatt caattttatt tacatcaata tttgctgtat caggattgct atctgcaaaa
      481 ataattttag caccgcgcag tacaaatgca ttgactgttg aaacaaaagt atatgatggc
      541 actataactt catcaccttc ttttatttca agcagtatag cacaagcttc taatgcatcg
      601 gtgcatgatg ttgtgagcaa tacttttttg aaattatatt tctgttgtag aaactgatga
      661 catcgttttg taaagttccc gtcaccggaa attttgcctg atgaaaatac ttcttttata
      721 tagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtttattg aatggaatca
      781 taatttttta ctgtctgata aatttcactt ttttgttaaa tgattcagat actgcatcaa
      841 gaatgtaaac acccttgctc agataaggaa gtttaaaact gaaaattttt aaacctgcat
      901 cagaaggacc attatataat gttgtcagtt ttttgcctgc agcatcgtaa agtaccaatt
      961 taatttctga acttacgggt aaatttaagt gaatagaaag taggtcatca attggattgg
     1021 ggctcacagt cagacttcca ttctccggca ttagtgaaat acatttttca ttattgtctg
     1081 ggtttttatc gctgctccca tttacatttt taattgtcac acaaatgtag tcgggcagtt
     1141 ttagcggatt tacagatatg cttgtgtgca atacataggt tttgtcagtt ccataaacga
     1201 ttgtttcatt agcattttcg ataaaagaac ctttgccttc cggttgaatt tgtaattcaa
     1261 aattatttac cgttgtattt cctgcattag aaagcaatgc agatagattt aaataattat
     1321 cgttgatatt ataactcaaa gattttagcc aaaggtcaac ataaggaaca ataacatcaa
     1381 aaggcttttg catttgcacc tcacacccaa aaatactgga tgcagttaat gtgataaaat
     1441 aacttcctgt atcactataa acatgcgaag ggctttcatt aggatctatt gctgtgccat
     1501 cgccaaaatc ccaggaataa gaagttgcac ctgttgtat
//
LOCUS       DAORQT010000342         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_17969_length_1522_cov_18.949557, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000342 DAORQT010000000
VERSION     DAORQT010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17969_length_1522_cov_18.949557"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1522
                     /locus_tag="PK134_08770"
     CDS             <1..>1522
                     /locus_tag="PK134_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB38982.1"
                     /translation="INFIVLFSFLLALGIVVDDAIVVIENTHRIFDNGKMDIVRAAKM
                     ATGEVFLPVLSGTLTTLAPFVPLAFWKGIIGKFMFFLPITLIITLLASLVVAYIINPV
                     FAVSFMKKHEEKHDPYKGLKITSIIFIAIALLFYVTGAFGMGNFTIFILLLNLLHRFW
                     LHKVIVRFQTKTWPAVQESYKRMLIKVLHRPRTMMAGTVGLLFLSFIAIGLRNGGVNF
                     FPIADPNFVYVFTTLPVGTDQAYTDSVTKILEGKVYNVLGEKNDVVKSVIASVGVNVS
                     DPSDEDQGYYANKSKIEVAFVEYSKRHGVSTSNYLEKIREAVKNTVPGAEVTVAQEQG
                     GPPVGKPISIEVSGDDLQAITTTSKSLKRYLDSLQIEGVEELKSDLQDSKPQIVFDIN
                     REHMNRLGIMTGTVGNEINMAVLGREASKYRDLNDEYKIMLRYKLDQRYDVSLLNNLK
                     ILYRDMSMGGLVRNVPLSSFATVKYDNTYGVIKRKNQKRVVTVSSNLLTDYQSREAE"
BASE COUNT          479 a          256 c          310 g          477 t
ORIGIN      
        1 aattaacttc attgtattat tctcttttct gcttgcatta ggaattgttg ttgacgatgc
       61 aattgttgtt attgaaaaca cgcatcgtat tttcgataat gggaaaatgg atattgttag
      121 agctgcaaaa atggcaacag gcgaagtttt cttacctgtg ctttccggaa cactcaccac
      181 acttgcacct tttgttccat tagcattctg gaaaggcatc attggtaagt ttatgttctt
      241 tcttcccatt acccttatca taacattact ggcatcattg gtggttgcat acatcatcaa
      301 cccagtgttt gctgttagtt ttatgaaaaa gcacgaagaa aagcatgacc catacaaagg
      361 tttaaaaatt acttcaataa tttttattgc aattgcactg ttgttttatg tgacaggtgc
      421 atttggcatg ggtaacttta caatcttcat tctgttgtta aatttacttc accgtttttg
      481 gttgcataaa gttattgtac gttttcagac aaaaacatgg cctgcagtac aggagtcata
      541 taaaagaatg ttgattaaag ttcttcaccg cccacgcact atgatggcag gaacagtagg
      601 attgttgttt ttatcattca ttgcaattgg tcttaggaat gggggagtaa attttttccc
      661 aattgcagat cccaactttg tttatgtgtt cacaacattg cctgttggaa ccgatcaggc
      721 ctatacagac tctgtaacaa aaattcttga aggtaaagtt tataatgtgt tgggcgaaaa
      781 gaatgatgtt gtaaaatctg ttattgccag cgttggtgta aacgtttctg acccatcaga
      841 cgaagatcag ggatactatg ccaacaagag taaaatagaa gtagcatttg ttgagtacag
      901 taaaagacat ggtgtttcaa ctagtaatta tttagagaaa atcagagaag cagtaaaaaa
      961 tactgttccc ggtgctgaag ttactgttgc acaagagcaa ggcggaccac ctgtaggcaa
     1021 acctatttct attgaagtga gtggtgatga tcttcaggca ataactacaa cttctaaaag
     1081 cttgaaacga tatcttgatt ccttgcagat tgaaggtgta gaagaattaa aatcagacct
     1141 gcaggatagc aaaccacaaa tagtgtttga catcaatcgc gaacacatga accgtcttgg
     1201 aattatgaca ggtaccgtag gaaatgaaat caatatggct gtacttggtc gtgaggcttc
     1261 aaagtaccgc gatttaaatg atgagtataa aattatgttg cgttataaac tggatcagcg
     1321 atatgatgtt agtctgctca acaatttaaa aatattgtat cgtgatatgt caatgggagg
     1381 acttgtgcgc aatgtaccgt tatcatcttt tgcaacggtg aaatatgata acacctatgg
     1441 tgtcataaaa agaaaaaatc agaagcgtgt agtaacagtt tcatcaaacc tgcttaccga
     1501 ttatcaaagt cgcgaagcag aa
//
LOCUS       DAORQT010000343         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18010_length_1520_cov_4.530375, whole genome shotgun sequence.
ACCESSION   DAORQT010000343 DAORQT010000000
VERSION     DAORQT010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18010_length_1520_cov_4.530375"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..565
                     /gene="efp"
                     /locus_tag="PK134_08775"
     CDS             5..565
                     /gene="efp"
                     /locus_tag="PK134_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRB38983.1"
                     /translation="MADTGDIGIGSVIRFNGDLYQVVEYQHRTPGNLRAFYQAKLRNL
                     KNGKLAENRFRSGEQVEVARVEYKTMQYLYKDGENLVVMDNETYEQHYIPEVMIGDSA
                     SYLKEEMMLKIAFEGDNPILAEPPTFVELVITYSEPGVKGDTATNTLKAATLETGAVV
                     QVPLFVNEGEKIKIDTRTGTYVERVK"
     gene            774..1208
                     /locus_tag="PK134_08780"
     CDS             774..1208
                     /locus_tag="PK134_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38984.1"
                     /translation="MTIDIPPGIILVEDDDIDRMVVTRAFAKNNIKNPLHIARDGEEA
                     LEMLKGLNGKEKLDPLPKIMLLDINMPRMNGLELLREIRHDEELQTISVFMLTTSKDD
                     QDRLEAYKNNVAGYIIKPVSFDKLVEAIAVLNTYWNLSVSPA"
     gene            1222..>1520
                     /locus_tag="PK134_08785"
     CDS             1222..>1520
                     /locus_tag="PK134_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB38985.1"
                     /translation="MSYKKINLLVVDDDEVDRIAIKRAIKSSGFNADLHFGDTYDEGM
                     EKALSMEFDVLIVDYYLTVMNAGDFISEYFQRGGTAPIIVVSSQGEEKIVVELMKA"
BASE COUNT          497 a          245 c          319 g          459 t
ORIGIN      
        1 gaatatggca gatacaggcg atataggaat tggttcagta atcagattca acggagattt
       61 atatcaggtt gtagagtatc agcatcgtac accgggcaac cttcgtgctt tttatcaggc
      121 aaaactgcgt aacttaaaaa acggaaaact tgcagaaaat cgcttccgtt caggtgaaca
      181 agtagaggtg gcacgtgttg aatacaaaac catgcaatat ctctataaag atggagagaa
      241 cttggtggta atggataacg aaacttatga gcagcattat attcctgagg tgatgatagg
      301 cgacagtgcc agctatttaa aggaagaaat gatgctgaag attgcttttg aaggtgataa
      361 tcctatactt gctgagccac caacatttgt tgaattggta attacttaca gcgaacccgg
      421 tgttaaaggc gatactgcaa caaacacttt aaaagcagca actttagaaa caggtgctgt
      481 cgttcaggtt ccgctttttg taaatgaagg tgaaaaaatt aaaattgata cgcgcaccgg
      541 cacctatgta gaacgcgtaa aataaaatct aaaaagtttt tacttaaaac ctcgcttctg
      601 tcgaggtttt tttattgccc tttttgaact ataaattata gttgaacctg ttttcttcaa
      661 tcttttccat caaaatttcc tgaaatctta catgttgctt tttgcaacat actggccatt
      721 cagtaaccaa tagtgctgat ggcagtataa gttgtattca aaaatgatca ggtatgacaa
      781 ttgatatacc accgggaata attttagttg aggatgacga cattgataga atggttgtta
      841 ctcgtgcatt tgccaagaat aatattaaaa acccattgca cattgctcgt gatggcgaag
      901 aagccttaga aatgcttaag ggcttgaatg gtaaagaaaa acttgacccc ttgcctaaaa
      961 ttatgttgct cgatattaat atgccgcgta tgaatggtct tgagttgctt cgtgaaatca
     1021 gacatgacga agaacttcaa accattagtg tttttatgct tacaacatcc aaagacgatc
     1081 aggacagatt ggaagcatat aaaaacaacg tagcaggtta tattatcaaa cccgtgtctt
     1141 tcgacaaatt ggttgaagct atagctgtat tgaatactta ttggaacctt agtgtgtcac
     1201 ccgcttaaat aattttttat tatgtcttac aaaaagataa acctgttagt tgttgatgat
     1261 gatgaagtag atagaattgc aattaagaga gcaataaagt catcaggatt taatgccgat
     1321 ttgcattttg gcgataccta cgatgaaggt atggagaaag cattgagtat ggaatttgat
     1381 gttttaattg ttgactacta tctgactgtg atgaatgcgg gtgatttcat ttcagaatat
     1441 tttcaacgtg gtggcacagc gccaatcatt gttgtatcat cacaaggcga agaaaaaata
     1501 gttgttgaac tgatgaaggc
//
LOCUS       DAORQT010000344         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18172_length_1510_cov_6.345704, whole genome shotgun sequence.
ACCESSION   DAORQT010000344 DAORQT010000000
VERSION     DAORQT010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18172_length_1510_cov_6.345704"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1458
                     /locus_tag="PK134_08790"
                     /pseudo
     CDS             83..1458
                     /locus_tag="PK134_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
BASE COUNT          526 a          246 c          316 g          422 t
ORIGIN      
        1 ttttactatt gcaaacgaat ttttaattgt ttcaaataaa ttcaaaagca attcataaac
       61 acatcaaaaa aaataaatta ttatgccaaa acattcatgg gcagaaccct ttaaaataaa
      121 aatggttgag ttgttgaaaa tgacaacacc acaacaacga aaaaagcatt acagcaagca
      181 ggttacaaca cgtttctact taaatctgaa gatgtatata ttgacttgtt gacagacagt
      241 ggaacttctg ccatgagcga tcagcaatgg gcaggaatga tgcttggtga cgaagcttat
      301 gccggcagta aaaattttta tcgcttagaa gatgtcgtaa aaaaatatta cggatttaaa
      361 catcttatcc ctacacatca gggccgtggt gcagaaaata ttctgtcgca gataatgatt
      421 aaaaaaggtg atgtggttcc gggcaacatg tattttacta caacacgcct tcaccaggaa
      481 cttgcaggtg caacattcta tgatgtcatt attgacgagg cacatgacag ctctaacctg
      541 catccattta aaggtaatat tgacatcaac aaattagata agttagttaa aaaatttggt
      601 gccgataaaa ttccttatgt ctgtgtagca actacagtta acatggccgg tggtcagcca
      661 atttctatgg ctaatattaa agagcttaga aaatatactg caaagcttgg cataaaggtt
      721 atgttggata tgacacgcat tgcagagaat gcctatttca tacaacagcg tgaagaaggt
      781 tataaaagaa aaagtattgc aaccatcgta aaagaaatct gcagcctgac tgatggtgca
      841 acatttagcg gaaagaaaga tgcattggtt aacataggag gctttctggc tttgaacgat
      901 gataaaaagt ttgaagaagc aagtaatctt gttgttgtat atgaaggact gcacacttat
      961 ggtggattag ccgggcgtga tatggaagca atggcaacag gcattattga atctgttagt
     1021 gatgatcata tcagggcgcg tatcggacag gtgctatatt taggtgataa gctcaatgaa
     1081 atgaatattc caattgtaaa tcctgtgggt ggacatggaa tttttattga tgctaaaaag
     1141 tttttgccgc atcttaaaca gacacagttt cccgcacagg cattagctgc tgaaatttac
     1201 gaagacagcg gtgtacgtac catggaaaga ggaattgtat ctgcaggcag aaataaaaaa
     1261 actggcgacc actattatcc cgaattagag ttggtgcgac tgactattcc acgcagggtt
     1321 tatacacagg cacacatgga tgtgatttca gaatcaatag agcgtgttta taaaaaccga
     1381 gaaaaaataa aaggattaga aatgatttat gaacctacct atctgcgatt ctttcaggca
     1441 aggttcaaaa aaagataagt gttttttgta attattgatt actaaaggag gctaagagcc
     1501 tccttttttg
//
LOCUS       DAORQT010000345         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18333_length_1500_cov_6.961938, whole genome shotgun sequence.
ACCESSION   DAORQT010000345 DAORQT010000000
VERSION     DAORQT010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18333_length_1500_cov_6.961938"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK134_08795"
     CDS             <1..703
                     /locus_tag="PK134_08795"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRB38986.1"
                     /translation="ERIECSVTITINERVSNDEYKGSIQITSRRPVFQTSYNSPTLNF
                     NDDNFQFKYIEYQPLEFSENGSNPNLTAVLAFYANIILGVDYDSFSLYGGAPYFAKAQ
                     TIVNNMQNAREAGWRAFEGTKNRYWLSENMTNPIFKPYHEMFYQYHLKGLDVMAKKKD
                     DGLSQISESLDLLMSVHKEKPGSLLLQTLFYAKADEIVNIYSGAYPDQKVKVSNLLNE
                     IDPANGTKYSKIMGN"
     gene            complement(733..1131)
                     /locus_tag="PK134_08800"
     CDS             complement(733..1131)
                     /locus_tag="PK134_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38987.1"
                     /translation="MIKVREFVFLILIGAVVLLSAFKIQQDGKDYGQCLQKVNKKFGA
                     ETLSCPASTKPYKVWFVNTCSDTLAVKLAVQEKSKRWRTFTRTQLLPGDTISGYACTG
                     VGKYLYYTKQYKDNSVDLPSDEVIDNSNKE"
     gene            complement(1128..>1500)
                     /locus_tag="PK134_08805"
     CDS             complement(1128..>1500)
                     /locus_tag="PK134_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB38988.1"
                     /translation="TYVANMDVANVNAILQQGFCNTILKKYTEAIGNFTDVIFYEPNH
                     EWAYVSRGSAYNKIEKYDKAIVDFNKALELKPEDAEAYNNRGFAKKMLGDIKGACEDW
                     NKSKKLGNEEAKIILKNNYCK"
BASE COUNT          469 a          265 c          259 g          507 t
ORIGIN      
        1 ggaaagaata gaatgttctg ttacaataac aataaatgag cgtgtttcaa acgatgagta
       61 taaaggaagc attcagataa cttcaagacg acctgttttt cagacatcat ataattcacc
      121 gactttaaat tttaatgacg ataattttca gtttaagtat atcgaatatc agcctctaga
      181 gttcagcgag aatggaagca atccaaatct gacagcagta cttgcttttt atgcaaacat
      241 tattcttggt gtggactatg acagcttttc attatatgga ggagcgcctt attttgccaa
      301 agcacaaact attgtaaaca atatgcagaa tgcacgcgaa gcaggatggc gtgcttttga
      361 aggcacaaaa aatcgctact ggctctctga aaatatgact aatcctattt ttaaacctta
      421 ccatgaaatg ttttatcagt atcatctgaa aggccttgac gtgatggcaa aaaagaaaga
      481 tgatggattg tcgcaaattt ctgaaagcct tgatttgtta atgtccgtac ataaagaaaa
      541 gccgggttcg ttgttattac aaactttgtt ttatgctaaa gcagatgaga ttgtaaatat
      601 ttattcagga gcctatcccg atcagaaagt aaaagtcagc aatctgctaa acgaaattga
      661 tcctgcaaac ggaaccaagt actcgaagat tatggggaat tgattgttgt agaattcaaa
      721 aagtaaaaat ttttattcct tattactatt atcaattact tcatcagaag gtaaatccac
      781 agagttgtct ttgtattgct ttgtatagta aagatatttt cctacgcctg tgcaggcata
      841 gcctgatatt gtatctccgg gtaaaagttg tgttcttgta aaagtccgcc agcgttttga
      901 tttttcctga acggctaatt taacggcaag ggtatcgcta cacgtattta caaaccaaac
      961 tttataaggc tttgttgatg caggacatga taatgtttct gcaccaaatt ttttatttac
     1021 tttttgtaag cactgcccgt aatcttttcc atcctgctgt attttgaaag cagataaaag
     1081 caccactgca ccaatcagta ttaaaaaaac aaactcacgc accttgatca tttacagtaa
     1141 ttatttttaa gaatgatttt agcttcttcg tttcctaatt tttttgattt gttccagtct
     1201 tcgcaagcac ctttaatgtc accaagcatt tttttagcaa agcctctgtt gttgtaggct
     1261 tctgcatctt caggtttcag ctctaatgct ttattaaaat ctactatggc tttatcatac
     1321 ttttcaattt tattatatgc actgcctctt gaaacataag cccactcatg attgggttca
     1381 taaaaaatta catcagtaaa gttgccaata gcttcagtat atttttttaa gatagtgtta
     1441 caaaatcctt gttgtaaaat tgcatttacg ttggctacat ccatgtttgc cacataggtg
//
LOCUS       DAORQT010000346         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18665_length_1483_cov_9.722689, whole genome shotgun sequence.
ACCESSION   DAORQT010000346 DAORQT010000000
VERSION     DAORQT010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18665_length_1483_cov_9.722689"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1289)
                     /locus_tag="PK134_08810"
     CDS             complement(297..1289)
                     /locus_tag="PK134_08810"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRB38989.1"
                     /translation="MKVAVVGATGLVGKTMMRILEERKFPVSEFIPVATANSAGKKVM
                     FSGKEYTVITCEEAVALKPDIALFSAGGSTSLEWAPRFAQVGTTVIDNSSAWRMNEKC
                     PLVVPEINADVLTKEDKIIANPNCSTIQMVMVLNPLHKKYKLKRIVVSTYQSVSGTGK
                     KAVDQLMNERKGISGAMAYPYNIDMNVLPHVDSFLENGYTKEEMKMVNETRKIMRSDK
                     IKVTATTVRVPVVGGHSEAVNAEFENQFELSDVRQLLSHTAGVTVIDDPAQLQYPMPT
                     YAEGKDDVFVGRIRRDESQPQTLNLWVVSDNLRKGAATNAVQIAEYLLKQFWQH"
BASE COUNT          436 a          296 c          214 g          537 t
ORIGIN      
        1 tcaacagaaa taacttttac atcattaata ccgttgtcat gaagcatttt ttctgtttaa
       61 taattgagtt ttaaaaaatc actatatccc ttagcaatag tccgttatct cattacttaa
      121 tttatcttat aactatctaa ctttatggga tcgttaattt aacataaatt caagactgtt
      181 caaatcaaat tgtaatttaa gtcaacaact gtcaatatca agttttttct atttcaatct
      241 gtcaaatagg taaagtattt attgtaaaca atttgaattt ttatcactta caagctttaa
      301 tgttgccaaa actgctttag caaatactct gcaatttgaa cagcattggt agcagcgcct
      361 tttctgagat tgtcagatac cacccaaagg tttaatgttt gtggttgtga ttcatcacgc
      421 ctgattcttc ctacaaaaac atcatctttt ccttctgcat aagtgggcat cggatactga
      481 agttgtgcag ggtcgtcaat aaccgtcaca cctgcagtat gtgataaaag ttgtcttaca
      541 tcactcaatt caaactgatt ttcaaactct gcattcaccg cttcgctatg gcctccaaca
      601 accggtacgc gcacagttgt tgctgttact tttattttgt cagagcgcat tatttttctt
      661 gtttcattaa ccattttcat ttcttccttc gtatatccat tttctaaaaa ggaatcaaca
      721 tgtggcaaca cgttcatgtc aatattatat ggataggcca tagcaccgga aattcctttg
      781 cgttcattca tcaactgatc aactgctttt tttcctgttc cggaaactga ctgataagta
      841 gaaacaacta tccttttaag tttatatttt ttatgtagcg gattaagtac catcaccatc
      901 tgaatagtag aacaatttgg gttggcaata attttatctt ctttagttaa tacatcagca
      961 tttatttcag gaactactaa agggcatttt tcattcatcc gccatgccga tgagttgtca
     1021 attacagtag ttccaacctg agcaaatctt ggagcccact ctaaagatgt tgatcctccg
     1081 gcagagaata aggcaatatc aggtttcaga gcaactgctt cttcacaagt tatcactgta
     1141 tattcctttc ccgaaaacat tactttttta ccggcagaat ttgccgttgc aacaggtata
     1201 aattccgata ccggaaactt tctttcttcc agaatgcgca tcatggtttt tccaaccaag
     1261 cctgtggcac caactacagc tactttcata atattttatt ccttcctcaa aaatgaataa
     1321 aaaacacatt acttaatgct gtttcaattt atttatttta aataatgagg tttgcttatc
     1381 ttcactgcct tatattttat tttaacatga agcgattact actttttttc gcaatgattc
     1441 cattttttgg tgttgcacaa ataacagata acttttctga tgg
//
LOCUS       DAORQT010000347         1475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18814_length_1475_cov_6.318310, whole genome shotgun sequence.
ACCESSION   DAORQT010000347 DAORQT010000000
VERSION     DAORQT010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18814_length_1475_cov_6.318310"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="gldN"
                     /locus_tag="PK134_08815"
     CDS             <1..780
                     /gene="gldN"
                     /locus_tag="PK134_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRB38990.1"
                     /translation="VLDGVYVKEHNPTRKVMEYSFLREADVLWAKRVWEVIDLKQKIN
                     LPLRFPREGDTKDRKSLIEVLMNAIDEGSLTAYSTTDDEFTTPMTLEEFKKIGGAGMD
                     TIQITDPEPPYLTRDSAIQRTFSRDKVIAYRIKEEWFFDKQRSVMECRIIGIAPLMLA
                     EDEYGNIREGNIQKPLFWIYYPEARKLLANAEAFNRENDTERRTFDDVFHKRMFNSYI
                     IKESNVYNRRIEDYKTGLDALLEAERIKSEIVNLEHDMWEY"
     gene            complement(835..>1475)
                     /locus_tag="PK134_08820"
     CDS             complement(835..>1475)
                     /locus_tag="PK134_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRB38991.1"
                     /translation="YSMELCGGTHVNATGEIGLFKILSESAVAAGVRRIEAITSIEAE
                     KYIINQLTQLNTIKTELKNPKDIVIRVKELLSENEILQRQIDGFVHEKAQLIKADLLK
                     NVKLQNGINVIAQKIDLTNADAIKNISFELRNQIPDSFILLASENDGKAMLSLIISDS
                     LVQSGNLNATTVIRNISKHIQGGGGGQPFYATAGGKNPAGIDAAIEEALKMI"
BASE COUNT          445 a          262 c          283 g          485 t
ORIGIN      
        1 gtattggatg gtgtttatgt taaagaacac aatcctactc gtaaagtaat ggaatattca
       61 tttctgcgcg aagctgatgt attgtgggca aagcgtgttt gggaggtaat agatcttaaa
      121 caaaagatta atctgccact tcgtttcccc cgtgaaggcg atacaaaaga cagaaaaagt
      181 ctgatagagg tgttgatgaa tgcaattgat gagggttcat taactgctta ttctacaact
      241 gatgatgagt ttactactcc aatgaccttg gaagaattca agaaaattgg tggagccggt
      301 atggatacta ttcagataac agatccggag cctccatatc taactcgtga ttcagccatt
      361 cagcgtactt tttcacgtga taaagttatt gcctatcgta ttaaagaaga gtggttcttt
      421 gacaagcagc gttcagttat ggagtgccgt attattggta ttgcacccct aatgcttgcc
      481 gaagatgaat atggaaatat cagagaaggt aatattcaaa aacctctttt ctggatttat
      541 tatccggaag caagaaagtt gcttgcaaat gctgaagcat tcaatcgtga aaatgataca
      601 gaacgcagaa catttgatga cgtgtttcat aagcgaatgt ttaacagcta tattattaaa
      661 gagtcaaatg tgtataacag acgtattgaa gattacaaaa caggtttaga tgctttgctt
      721 gaagcagaga gaattaaatc agagatagta aatcttgaac acgacatgtg ggaatattaa
      781 tttctaataa catataaaag agcgaggctg cccattcagg cagcctcgct ctttttatat
      841 catctttaac gcttcttcaa ttgcggcatc aattcctgca ggatttttac caccggcagt
      901 tgcataaaat ggttgtccac caccgccacc ttgaatgtgt ttagaaatgt ttctgataac
      961 tgttgtggca ttcaagttgc ctgattgcac caaactgtct gaaattatca atgataacat
     1021 ggctttgcca tcattctcag aggctaataa aataaatgaa tcaggtatct gattacgaag
     1081 ttcaaaagaa atgtttttaa ttgcatcagc attagttaag tcaattttct gtgctataac
     1141 attgatgcca ttctgcaact ttacattttt tagaaggtca gctttaatga gctgtgcttt
     1201 ctcatgcaca aagccgtcta tttgcctttg cagtatttca ttttctgaca atagttcctt
     1261 tactcttata acaatatctt taggattttt tagttctgtt ttaatcgtgt ttagctgtgt
     1321 caattgattt atgatatatt tttcagcctc tattgatgtt attgcttcaa tacgtcttac
     1381 acctgcagcc actgccgact cacttagaat tttaaataag cctatttcac cggttgcatt
     1441 aacatgtgtg cctccacata gctccatact gtatg
//
LOCUS       DAORQT010000348         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18910_length_1470_cov_4.556184, whole genome shotgun sequence.
ACCESSION   DAORQT010000348 DAORQT010000000
VERSION     DAORQT010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18910_length_1470_cov_4.556184"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PK134_08825"
     CDS             complement(<1..1182)
                     /locus_tag="PK134_08825"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRB38992.1"
                     /translation="MTSSVKNADGTRTDTWKQSLPAAPYLTMMAVSNFKVVKDRWRNI
                     EVNYYVDPEYEMYAKDIFGNVPEILDFYSRKLGVDYPWEKSSHVVVHDYVSGAMENTS
                     AILYGEFLQRTKRELLDGTSESTIAHEIFHHWFGDYVTCESWSNIPLNESFATYGEYL
                     WDEYKYGREEADKGGMDDLNQYLAEAKYKQVDLIRFHYNAREDMFDRHSYSKGGRVLH
                     MLRKYVGDDAFFASLKKYLNDNKFSPVEAHNLRLAFEAVTGQDLNWFFNQWFFAKGHP
                     IIDISYQWNDTLKKQTVIIEQKQNLQETPLYKLPLAIDIYENGKVRREQVTLTQQKEY
                     LFFDCNSKPQLINVDAEKQLLCAKKDNHTSDEWRYQYQHAPLFLDRYEAIDALAKGYE
                     GN"
BASE COUNT          412 a          291 c          276 g          491 t
ORIGIN      
        1 attgccttca taacctttgg ctaatgcatc aatagcttcg taacggtcga gaaacaatgg
       61 cgcatgctga tactgatacc tccattcatc gctggtgtgg ttgtcctttt tagcgcataa
      121 caattgtttt tctgcatcta catttatcaa ttgtggcttg ctgttgcaat caaaaaataa
      181 atattctttt tgttgtgtaa gtgtaacctg ttcgcgtcta acttttccgt tttcataaat
      241 atctattgca agaggaagtt tatagagtgg tgtttcctga agattttgtt tttgttcgat
      301 gatgacggtt tgttttttca gagtatcgtt ccactgataa ctgatatcaa ttatgggatg
      361 acctttagca aaaaaccact gattgaaaaa ccagttgagg tcttgtccgg taacagcttc
      421 aaaagccaaa cgcagattat gtgcttcaac agggctgaat ttattgtcgt ttaaatattt
      481 ttttaatgat gcaaaaaagg catcatcgcc aacatattta cgaagcatgt gtaacacccg
      541 accccctttt gaatagctgt gtctgtcgaa catatcttca cgagcattgt aatgaaagcg
      601 aataagatca acctgtttgt atttggcttc agcaagatat tgattcaaat catccatgcc
      661 gcctttgtca gcttcttcac gaccgtattt gtattcatcc cacaggtatt cgccataagt
      721 tgcaaacgat tcgtttaatg gtatgttgct ccacgactca cacgttacat aatcgccaaa
      781 ccaatgatga aaaatttcat gtgcaatcgt actttcactt gtgccatcaa gcagttcgcg
      841 ttttgttctc tgtaaaaact caccataaag aattgcagaa gtattttcca tggcaccact
      901 cacataatca tgaacaacaa catgtgatga tttttcccat ggatagtcaa cgcctaattt
      961 acgagagtag aaatcgagta tttcgggcac gtttccaaaa atatcttttg catacatttc
     1021 atattccggg tcaacatagt agttgacttc aatgttgcgc caacggtctt taaccacttt
     1081 aaaattgcta acagccatca tcgtcagata gggtgcagca ggcaatgact gtttccatgt
     1141 atctgtacgt gtaccgtcag catttttaac tgaagaagtc atcaagccgt tgctaagtgt
     1201 tacaatgagc tgtcaacagt tatgctgatt tcctgagtca tgttttgatt tggagaatca
     1261 atagtaggga accaaacgga gttgctttgt gtttcgcctt gtgtccaaac ctgtcgtggt
     1321 ttattgggat tttttccatc ggcatttaca aagtaaagac ctttatcact attaattgct
     1381 acactgccac caatgttttt tagttcatca ggctttgata tatagtcaat ataaactgat
     1441 tgctgctgat gtcaatggag acaaggtgat
//
LOCUS       DAORQT010000349         1466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18987_length_1466_cov_5.123317, whole genome shotgun sequence.
ACCESSION   DAORQT010000349 DAORQT010000000
VERSION     DAORQT010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18987_length_1466_cov_5.123317"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1208
                     /locus_tag="PK134_08830"
     CDS             66..1208
                     /locus_tag="PK134_08830"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRB38993.1"
                     /translation="MHNKVQLKFSLALLLTCFSVSDTFSQCCAGGSGSPIAGGTSQGV
                     LQEKQVEISTNFQFISTDKFYKKNKVAPDSVRTFDGFNSTYQYFRLAYGITKSLTFSV
                     ESGYYYQKKETGLHKDAATTYNAKGFGDLILFPRYDIINWTEEKHRTEVTVGLGFKIP
                     LGSYNDSTGNVEPFSGYTYYVTNPTSVQLSSGAHDIIFYTFLYRGFTEKNFRVFANAL
                     YIKKGYNPNGEKLGDFASVGIFAGKTFFNNLGVTLQARYEWMDRMKVNESISLFGKPS
                     NYFPKATGYKKIFISPQLSYTKGKFTVYGSYDIPVYQYLNTSEHYTQMGSQHQATLGV
                     TFRFMALNSYLKEKSGVTYVCPMHPNVISDKPGKCPDCGMDLVKKK"
     gene            1317..>1466
                     /locus_tag="PK134_08835"
     CDS             1317..>1466
                     /locus_tag="PK134_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB38994.1"
                     /translation="MLFVQPSYLIRLFYSGLLWRMPTRQPIVYLTFDDGPEPEATPFV
                     LSQLKK"
BASE COUNT          485 a          250 c          255 g          476 t
ORIGIN      
        1 tgataaaatc caactaacag aataatagtt ttacagttgg agtaaatttt gtaaattaaa
       61 tatctatgca taataaagtt caactgaaat tttctttggc tctgttatta acttgcttct
      121 ccgtttctga taccttttcg caatgttgtg caggtggaag tggcagccca attgccggag
      181 gaacttcaca aggtgttctt caggaaaaac aagttgaaat aagtactaat tttcaattta
      241 tcagcaccga taaattttat aaaaaaaata aagttgcacc tgattcagta cgaacatttg
      301 atggttttaa tagtacctat caatatttca gattggctta tggtattaca aaaagcctaa
      361 cattttctgt tgagagcggc tattattatc aaaagaaaga aacaggtctg cataaagatg
      421 cagccacaac atacaatgca aaaggttttg gtgatttaat tctttttccg cgttatgata
      481 ttattaactg gacggaagaa aagcatcgta ctgaagttac cgttggttta ggatttaaaa
      541 ttcccctcgg aagctataat gattcaacag gcaatgtaga gccattttcg ggatacacct
      601 attatgttac caaccccaca tcggtacaac tttcatcagg cgcacacgat attatttttt
      661 acacttttct ttacagagga tttacagaaa aaaatttcag agtttttgct aatgccttgt
      721 acattaaaaa aggatataac cctaatggag aaaaattggg tgactttgcc agtgtaggaa
      781 tttttgcagg taaaacattc ttcaacaact taggtgtaac cttgcaggca cgatatgaat
      841 ggatggacag aatgaaagtt aatgaatcta tttctctgtt tggaaaacca tcgaattatt
      901 ttcccaaagc tacgggttat aaaaagattt ttatatcacc acaactcagc tataccaaag
      961 gtaagttcac ggtttatggt tcttatgaca ttcccgttta tcagtactta aatacatcag
     1021 aacactacac acaaatgggt tcacaacatc aggcaacact tggtgttact ttccgcttta
     1081 tggctttaaa tagttatcta aaagaaaaat caggagttac ctatgtatgc cccatgcatc
     1141 cgaatgttat ttcagataaa cccggtaagt gtcccgattg cggaatggat ttggttaaga
     1201 aaaaatagag ttacttttta ttattctgat tttatacttt cacatataac tattcatcca
     1261 acaaagacat gatcaggttg tttatttttg gcaaaaagaa ggaatagtat tcataaatgt
     1321 tatttgttca accatcatat ttaatcagac ttttttattc aggattgtta tggcgtatgc
     1381 caacgcgaca accaatagtt tacctgacat ttgatgatgg tcctgagcct gaggcaacac
     1441 ctttcgtatt gtcgcagtta aagaaa
//
LOCUS       DAORQT010000350         1465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_18999_length_1465_cov_6.614894, whole genome shotgun sequence.
ACCESSION   DAORQT010000350 DAORQT010000000
VERSION     DAORQT010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18999_length_1465_cov_6.614894"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..>1465
                     /locus_tag="PK134_08840"
     CDS             181..>1465
                     /locus_tag="PK134_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB38995.1"
                     /translation="MYFVANVTLAQDIFMSNGVVSGCSGTFYDSGGDSTNYSNNENFV
                     YTICSDQPGKCVQLSFQSFAIEKDFDFLFIYNGPSTSSPLLGTFTGTNSPGTITATSG
                     CLTIRFSSDYTVNRGGWKAYVNCINCPVNGCPTCNGGAPPPNDACSGAQNLGLLPQPA
                     ACPNGIGAVNLTNTSNLCATAEIPYQSLQGCKPIGSMSNPATDVWYKFSLTAPVLDIT
                     INGLITPQVGLYSGTGCGNLVPRGCAIGGGGYLHTTFGGLAAGTYYLQVSGGNINDQC
                     NFSLSLQNNFDCSGCVMQSGLTVSPPPSNGFYLSGQQVTFCLNVTDFNPTSANWLHGI
                     VPKFGPGWDTTTIIALPPASCSNNGFWSWYSNPIISSANNKPYGPGFFYETSAGNSSN
                     TQDNNPGNNFGDNLGQNCNLNFCWKINTLPQSLCNF"
BASE COUNT          405 a          295 c          284 g          481 t
ORIGIN      
        1 tactatatcg aagcaagcaa aaatcctctt aagttgttgt agtaattact ctatattgtc
       61 acttattaca tgagtgctgt tgaagttccc acaggtgtaa gttattaaca atttattgtt
      121 agctttacgc ttcatttaaa aggtatttcc aaattgccga gaattattgc tttttgcgta
      181 ttgtattttg ttgcaaacgt tacgctggcg caggatattt ttatgtccaa tggcgtggtg
      241 agcggttgca gcggaacttt ctatgacagc ggtggtgact caaccaacta cagtaataat
      301 gaaaatttcg tttataccat ttgcagtgat caacccggca aatgtgtaca gttaagcttt
      361 cagtcatttg caattgaaaa agattttgat tttcttttta tctataatgg cccttctact
      421 tcatctcctc ttttaggtac atttacagga accaactctc ctggtacaat aacagcaaca
      481 tcaggttgtt taacaattag attttcttct gattacactg ttaacagagg tggatggaaa
      541 gcatacgtta attgtattaa ttgtcctgtt aatggttgtc ccacatgtaa cggtggtgct
      601 cctccgccaa acgatgcttg ttcaggtgca cagaatcttg gattattgcc acaacctgcg
      661 gcatgcccca acggcattgg cgcagttaat cttacaaata catctaacct ttgtgcaact
      721 gcagaaattc catatcagtc gcttcagggt tgtaaaccaa ttggcagtat gtcaaatccg
      781 gctactgatg tttggtacaa attcagtctg acggcacctg tacttgatat aaccatcaat
      841 ggtttaataa caccacaggt tggattatat tccggaacag gatgcggaaa tttagttcca
      901 cgtggttgcg ctattggcgg tggtggttat ttgcatacca cttttggtgg tcttgctgca
      961 ggcacctatt atcttcaggt cagtggtggc aacatcaatg atcaatgtaa tttttcttta
     1021 tctcttcaaa ataatttcga ctgtagtggc tgtgtaatgc aaagtggact gacggtttca
     1081 cctccacctt ctaatggatt ttatctttca gggcaacaag taacattttg tttgaatgtt
     1141 accgacttca atcctacttc tgcaaactgg cttcatggca ttgtacctaa atttggtccg
     1201 ggatgggaca ccacgacaat aattgcattg ccaccggcat catgttccaa caatgggttc
     1261 tggtcatggt actcaaatcc aataatctca tcagccaaca acaaacctta cggtccggga
     1321 tttttttatg aaacttctgc aggtaatagc agcaatactc aagataataa tcccggaaat
     1381 aatttcggtg ataaccttgg acagaattgt aatctcaatt tttgctggaa gataaataca
     1441 ctacctcaat cattatgcaa ctttg
//
LOCUS       DAORQT010000351         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_19041_length_1463_cov_9.900568, whole genome shotgun sequence.
ACCESSION   DAORQT010000351 DAORQT010000000
VERSION     DAORQT010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19041_length_1463_cov_9.900568"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PK134_08845"
     CDS             complement(<1..165)
                     /locus_tag="PK134_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB38996.1"
                     /translation="MFIQQLYTSCLAEAAYYIESDGQAAIIDPIRETTPYLSLSEQRH
                     ARITHIFETHF"
     gene            complement(229..1116)
                     /locus_tag="PK134_08850"
     CDS             complement(229..1116)
                     /locus_tag="PK134_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRB38997.1"
                     /translation="MNRFSIKDIEILTGIKPHTIRIWEQRYGIIKPQRTATNIRYYTE
                     EDLKLMLNVSLLNKMGFKISKITCLKPNEIEHKVLSLVEQANDADINLSALLNCMVRL
                     DEDTFEHLLNKYLETNGLESTMTRILFPFLYRIGLLWQTGSITPAYEHFFTNLVRQKI
                     IAAIDKQVLSPLPDAKRFLLFLPPNEPHELGLLYAHYVIRQRGHRSVYMGLNLPIDHI
                     ICAHECCQPDFIVTTLTSCIKINAIEEFIVQISKTFSKSKILLSGAQAVNRSLHLPQN
                     VQVISDFDDFIQYIENRVN"
     gene            complement(1189..>1463)
                     /locus_tag="PK134_08855"
     CDS             complement(1189..>1463)
                     /locus_tag="PK134_08855"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRB38998.1"
                     /translation="KGEQNSCHCTFQPISNKKQTAILPKTFFIDIPDDLIQEIEKCKS
                     DDAVKQAGIDWAVTQAKELIKFGVPCLHFYTMGNPENIRKIASQLF"
BASE COUNT          452 a          259 c          260 g          492 t
ORIGIN      
        1 aaaatgtgtt tcaaaaatat gtgttatgcg tgcatggcgt tgttccgaca atgacaaata
       61 aggtgttgtt tcacgaatgg gatctataat tgcagcctgc ccatcagact ctatataata
      121 ggccgcttcg gctaggcaac ttgtatataa ttgttgaata aacatatttc tatcaggatt
      181 taaaacaaag ctacaatttt tacagtaatg aataaaataa agtatgcatt aattgactct
      241 gttttcaatg tattgaataa agtcatcaaa gtcagagatt acctgaacat tttgtggcag
      301 atgcaaactt cggtttactg cctgtgcacc ggaaagtaat attttacttt ttgaaaatgt
      361 ttttgaaatc tgtacaataa attcttcaat agcgttaatc tttatgcatg atgttaatgt
      421 agtcacaata aaatcgggct gacaacattc gtgagcacaa atgatatgat ctatcggtaa
      481 gttaagaccc atataaactg atctgtgacc acgttgtctg attacatagt gtgcatacaa
      541 caaccctaat tcatgcggtt cgttaggtgg cagaaagagt aaaaatcttt ttgcatcagg
      601 cagtggcgac aatacctgct tgtctattgc tgcaattatt ttctgacgta caagattggt
      661 aaaaaaatgt tcgtaggccg gtgtaatgga gcctgtctgc caaagcaatc ctattctgta
      721 taaaaagggg aacagaattc ttgtcattgt actttccaac ccatttgtct ccagatattt
      781 atttagcagg tgttcaaaag tatcctcatc aagcctgacc atacaattca acaatgcact
      841 gaggtttata tcagcatcat tggcctgttc tactaacgac aaaactttat gttcgatttc
      901 attcggttta agacaagtta ttttcgaaat cttaaatccc atcttattca acaaagaaac
      961 attgagcatc aacttcaaat cttcctctgt gtaataacgg atattggttg cagttcgctg
     1021 tggtttaata ataccataac gctgctccca tattcgaatg gtatgcggct taattcctgt
     1081 aagaatttct atgtctttaa tcgaaaaccg gttcaactga gtggagtttg tgatataaac
     1141 acctgtcgtg tttaaaagtt tcagccttat gtatggatgc ttgaaacatt aaaacaattg
     1201 tgatgcaatt tttctgatat tctccggatt gcccatagta taaaaatgaa gacagggcac
     1261 accaaattta ataagttctt tagcttgtgt caccgcccaa tcaatacctg cttgttttac
     1321 tgcatcatca ctcttgcatt tttcaatttc ctgaatcagg tcatcaggta tatcaataaa
     1381 aaaagttttt ggtagtatgg cagtttgttt tttatttgaa attggttgga aagtacaatg
     1441 acaagaattc tgttcccctt ttt
//
LOCUS       DAORQT010000352         1458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_19152_length_1458_cov_4.167498, whole genome shotgun sequence.
ACCESSION   DAORQT010000352 DAORQT010000000
VERSION     DAORQT010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19152_length_1458_cov_4.167498"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PK134_08860"
     CDS             <1..1449
                     /locus_tag="PK134_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRB38999.1"
                     /translation="DIIILHTFASLKNQTKYMLKRYIMLSLLWIAPWITSATNNTTGH
                     FTLPAGISSEDYQQGKVIFKVKDSYRSLCTATGINIQPLNVALNNIGCTAVSKMFPGK
                     EKPTTERNAMHQQYADLSLIYVAQINPSASIEKAINAMLATGVLIYAEPSYIYKTNFT
                     PNDGQIGSQWFLTKIQAFSAWDTQQGDTSVVIGIVDSGTDWDHPDLQNNIKYNYADPI
                     NGVDDDNDGYIDNYRGWDMSMNDNNPMVDMSDHGSHVSGCAAATTNNGTGVASPGFNC
                     KFLPVKCANATSVTTIDRGYEGIVYAADHGCQIINCSWGGSAGGSVGQDAITYATVNK
                     GALVVCSAGNTYTDIAQYPAAYNYAFSVAATNSSDEKSSFSTYNYSVDISAPGSNIFS
                     TLYNNSYASLDGTSMSAPITSGCAAIVKSQFPTYNNMQVAEQLRMTADNIYGVSGNSG
                     FVDKLGTGRVNLYNAVTQSPPRLIFQESLCCA"
BASE COUNT          443 a          306 c          291 g          418 t
ORIGIN      
        1 gacataatca ttttacatac attcgcatct ctaaaaaatc aaacaaaata tatgcttaaa
       61 cgctacatca tgctttccct tttatggatt gcgccatgga ttacatcagc cacgaacaac
      121 acaacaggtc attttacact tcctgcaggt atttcatctg aagactacca acaaggaaag
      181 gttatcttta aagttaagga ctcttaccgg tcactttgta ctgctacagg cataaacata
      241 caaccactga atgtggcttt aaataacata ggctgtacgg ctgtttctaa aatgtttccc
      301 ggcaaagaga agccaactac tgaaagaaat gctatgcatc agcagtatgc cgatttaagt
      361 ttaatttatg tggcgcagat taatccttct gcttccatag agaaagccat caatgcaatg
      421 cttgcaacag gtgttttaat ttatgctgaa ccatcttaca tctataaaac aaactttaca
      481 cccaatgacg ggcagatagg ctcgcaatgg tttcttacaa aaattcaggc ttttagtgca
      541 tgggatactc aacaaggcga cacctctgtg gttataggca ttgttgactc aggcacagac
      601 tgggatcacc cggatcttca gaacaatatt aaatacaact atgcagaccc aattaatggt
      661 gtggatgatg acaatgacgg atacattgac aactaccgtg gctgggacat gagcatgaac
      721 gacaacaatc caatggtgga tatgagtgat catggctcgc atgtatcagg atgtgctgct
      781 gctacaacta ataatggaac aggtgttgcc tcgcccggat ttaattgtaa gttcttaccg
      841 gtgaagtgcg ccaatgccac ctccgttact acaattgaca gaggttatga aggcatagtt
      901 tatgctgccg atcatggatg ccagattatc aattgctcat ggggtggcag tgcaggtggc
      961 agtgttggac aagatgctat tacctatgca acagtaaata aaggtgcctt ggttgtttgt
     1021 tctgcaggaa acacctacac cgatattgca caatatcctg ctgcatataa ttatgcattt
     1081 agtgttgctg caacaaactc cagcgatgaa aaatcatctt tttctaccta taattattct
     1141 gtagatattt ctgcacctgg ctcaaatatt ttctctaccc tatataacaa cagttatgct
     1201 tcgttagatg gaacttcaat gtcggcacca attacctcag gttgcgcagc catagtaaaa
     1261 tcacaattcc cgacctataa caacatgcag gtggcagagc aattacgaat gactgccgat
     1321 aatatttatg gtgtttcggg taacagtggt tttgttgata aattaggtac cggacgtgtg
     1381 aatctttata atgcagttac acaaagccct ccaaggctta tttttcagga aagcctctgc
     1441 tgcgcataag tgctttaa
//
LOCUS       DAORQT010000353         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_19295_length_1451_cov_4.456304, whole genome shotgun sequence.
ACCESSION   DAORQT010000353 DAORQT010000000
VERSION     DAORQT010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19295_length_1451_cov_4.456304"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PK134_08865"
     CDS             <1..584
                     /locus_tag="PK134_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRB39000.1"
                     /translation="VGQTSLGTSIFYSFWVVYFIILAYKLFIAYALFVNPYLIKKILT
                     GICSFPVLKRFRKGAVETGDQLIIASNGLKDKNLKYWMLSLLATFASWTARYSIVNCL
                     IHAFHGQTALSDLIVYGKQVIMGIIILLSPTPGGSGIAELMFTDFLGEFIHKGLAPTL
                     GILWRLISYYPYLIVGALILPRWIRERIVKKIF"
     gene            589..801
                     /locus_tag="PK134_08870"
     CDS             589..801
                     /locus_tag="PK134_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39001.1"
                     /translation="MSNKRRIFLIVISVTIGMGLSVIIFLMKNKGKALSDMDYLWLGT
                     NLFFSLLIVGGIGLYFMKKKDNDLMK"
     gene            837..>1451
                     /locus_tag="PK134_08875"
     CDS             837..>1451
                     /locus_tag="PK134_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRB39002.1"
                     /translation="MKKQTLVVALTIVFGLMTFAVQAHNEGKQVTYQAGNTNCNGYVA
                     YNAHIPKMPVVLVIPEWWGCNEYAKMRADMLASMGYFAFAVDMYGDGYIAPTPKEAGE
                     QASKFYTDNALAMERINAAIQKLKDFPMADVENMAIIGYCFGGSMALNAARANLPVKA
                     VVSFHGGLKGIANPAEIKPKILICHGGSDNFVSAEEVTAFKAEMD"
BASE COUNT          439 a          224 c          309 g          479 t
ORIGIN      
        1 atgtggggca aacatcattg ggtacaagca ttttttattc tttctgggta gtatatttta
       61 tcattcttgc ctataaactt tttattgctt atgcactttt tgtaaatcca tatctgatta
      121 aaaaaatatt aacgggcatc tgttcttttc cggtcttgaa acgatttaga aaaggtgcag
      181 ttgaaacagg cgatcaactt atcattgcct caaacgggtt gaaagataaa aatctgaaat
      241 actggatgct ttcattactg gcaacgtttg cttcgtggac agcaagatat tctattgtaa
      301 attgtctgat tcatgcattt cacgggcaga ccgcattgtc tgatttgatt gtttatggta
      361 agcaggtaat tatgggaata ataattttac ttagccccac acctggtggc agtggcattg
      421 ccgaactgat gtttaccgat tttcttggtg agtttattca caagggactg gcacctacat
      481 taggaatttt atggcggttg ataagttatt atccttacct tattgttggt gcactaattt
      541 tgccacgttg gattagagag cgtattgtaa aaaaaatatt ttagggttat gagtaataag
      601 cgaagaattt ttctgattgt catttcagta actattggaa tgggtctttc cgtcatcatt
      661 tttttaatga aaaataaagg aaaagcatta agcgatatgg actatctttg gttaggaacc
      721 aatctgtttt tttcattgct gattgttggc ggcataggac tttattttat gaagaagaaa
      781 gacaatgatc tgatgaaata agtaagtatt agttaagtaa tttaaaaatc aaaacaatga
      841 aaaagcaaac tttagtagta gcattaacaa tagtttttgg cttgatgaca tttgcagtgc
      901 aagcacacaa tgaaggcaag caagttacct accaagcggg caacaccaat tgcaatggtt
      961 atgttgccta taatgcacac attccaaaaa tgcctgttgt attagtcata cctgaatggt
     1021 ggggttgtaa tgaatatgct aagatgcgtg ccgacatgct tgcttctatg ggatattttg
     1081 cttttgccgt tgacatgtat ggtgatggct atattgcacc tacaccaaaa gaagcaggtg
     1141 agcaagcttc aaaattttat accgataatg ctttggctat ggagcgcatt aacgctgcaa
     1201 ttcaaaaact aaaagatttt cctatggccg atgttgagaa tatggccatt ataggatatt
     1261 gttttggagg cagtatggca cttaatgcag cacgtgctaa tttacctgta aaggcagttg
     1321 taagttttca tggtggatta aagggcattg ccaatccggc agagataaaa ccaaaaatat
     1381 taatctgtca tggtggcagc gacaactttg tttctgcaga agaagtgact gcttttaaag
     1441 ctgagatgga t
//
LOCUS       DAORQT010000354         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_19495_length_1441_cov_4.187590, whole genome shotgun sequence.
ACCESSION   DAORQT010000354 DAORQT010000000
VERSION     DAORQT010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_1441_cov_4.187590"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PK134_08880"
     CDS             <1..959
                     /locus_tag="PK134_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRB39003.1"
                     /translation="NSNTTNVCIANCKFCNFFRVPGHKEAYITSIEEYKVKIEEMFRY
                     GGDQLLLQGGHHPELGLSFYTGIFSELKKLYPQVKLHALGPPEVAHITKLEGMTHTEV
                     LKALKDSGLDTLPGAGAEILSDRVRRMISKGKCTGREWLDVMRAAHQLNITTSATMMF
                     GHIETLQERFEHLVMLRQVQSEKPAHAKGFLAFIAWPFQDENTILKRVKGIRNKVTSE
                     EFIRMIALSRIMLPNIKNIQASWLTVGKQTAQVALHAGANDFGSIMIEENVVSVAGAP
                     HRFTSQGIQDAIREAGFIPQLRSQQYQFREIPEMMEQQVINY"
     gene            964..>1441
                     /locus_tag="PK134_08885"
     CDS             964..>1441
                     /locus_tag="PK134_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRB39004.1"
                     /translation="MRKISIFFLLLFGLQMEAWATHNRAGEITFNQISLLTYRVQIVT
                     YTKTSSPADRPMLEMNWGDGTQDSLPRISKTTVGIDISRNYYEGVHTFPGPAVYTVFF
                     EDPNRNGGVVNIPGSVNIPFYVESQIVINPVLGYNNSPVLLQPPIDNGAVGKIFIHN"
BASE COUNT          445 a          256 c          324 g          416 t
ORIGIN      
        1 gcaattctaa tacgacaaat gtatgtattg ccaattgtaa gttttgtaat tttttcagag
       61 tgccggggca taaagaagca tacatcacca gcattgaaga atataaggtt aagattgaag
      121 agatgtttcg ttatggtggc gatcagttgt tgctacaggg aggtcatcat ccggaattag
      181 gtttgtcatt ttataccggt attttcagtg agttaaagaa actttatccg caggtaaaac
      241 tacatgcact tggcccaccg gaggtggcac atatcactaa gttagaaggt atgacgcata
      301 ctgaagtcct taaagcctta aaagattcag gacttgatac cttgccgggt gcaggagcag
      361 aaattttgag tgacagagta cgcaggatga tttctaaagg aaaatgtaca ggtcgtgaat
      421 ggcttgatgt gatgcgggct gcacatcagt taaacattac cacatctgca acaatgatgt
      481 ttggtcatat agaaacattg caggagcgtt tcgaacattt agtcatgctc agacaggtgc
      541 agtcagaaaa acctgcacat gcaaaaggtt ttcttgcctt tattgcatgg ccatttcagg
      601 atgagaatac aattttaaag agagtaaaag gtattcgcaa caaagttact tccgaagagt
      661 ttatcagaat gattgcattg agcagaatta tgttgcctaa catcaaaaac attcaggcat
      721 catggctcac tgtaggaaag caaacggcac aagtggccct gcatgcagga gcaaacgatt
      781 ttggctctat catgattgaa gaaaatgtag tgagtgttgc cggtgcacca catcgtttta
      841 catcacaagg tattcaggat gctattcgcg aagcaggatt tattcctcaa ttgaggtcac
      901 aacaatatca gttcagagaa atacctgaaa tgatggaaca acaggtaatc aactactgat
      961 tgtatgagaa aaatttcaat cttttttttg ctgcttttcg gtttgcaaat ggaagcatgg
     1021 gcaacacaca atcgtgcagg tgaaataact ttcaatcaga tttcattgct tacttacaga
     1081 gtgcagattg ttacttatac caaaacgagc agtcctgcag acagacctat gcttgaaatg
     1141 aattggggtg atggcacgca ggattctttg ccgcgtataa gtaaaactac agtgggcatt
     1201 gacatcagca gaaattatta tgaaggtgtt cacacttttc ccgggcctgc agtttatact
     1261 gtgttttttg aagacccaaa ccgcaatggt ggtgttgtaa atattccggg ctctgtaaat
     1321 attccatttt atgtagagtc gcaaattgtc atcaatcctg tgctgggata taataattca
     1381 cctgtgctgt tgcagccgcc aattgataat ggagccgttg gaaaaatatt tattcataat
     1441 g
//
LOCUS       DAORQT010000355         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_19709_length_1430_cov_5.693818, whole genome shotgun sequence.
ACCESSION   DAORQT010000355 DAORQT010000000
VERSION     DAORQT010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19709_length_1430_cov_5.693818"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK134_08890"
     CDS             complement(<1..252)
                     /locus_tag="PK134_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRB39005.1"
                     /translation="MSRVLVIRFSSIGDIVLTTPVLRCIKKQLPNAEVHFLTKRDFSD
                     VLANNPYIDNTFYVDNNLPEIIRQLKMVGYDYIVDLHHNM"
     gene            339..>643
                     /locus_tag="PK134_08895"
     CDS             339..>643
                     /locus_tag="PK134_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRB39006.1"
                     /translation="MIFTDTHTHLYATEFDADRKELMQQAIDAGVSRFFLPNVEKSSV
                     NEMLELCKQFPENCFPMMGLHPCSVKEDYLAELALVESELSKGKYFGVGEIGLDFYW"
     assembly_gap    644..696
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <697..1103
                     /locus_tag="PK134_08900"
     CDS             <697..1103
                     /locus_tag="PK134_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRB39007.1"
                     /translation="CELNLPVSIHSRSATTRAIELIQLSGLSVKGVFHCFSGSIEEAK
                     AITDMGMYLGIGGVVTYKNSGLDAIVSQVGLKHIVLETDAPYLPPVPHRGKRNIPEYL
                     LLTAQKLAVIKNCSVAEVAEQTTNNSKVIFKQ"
     gene            1100..>1430
                     /locus_tag="PK134_08905"
     CDS             1100..>1430
                     /locus_tag="PK134_08905"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRB39008.1"
                     /translation="MIKTDKKNLLVIYTGGTIGMIHDEETGSLKPFNFGKLSTVVPEL
                     KKLPCKIDFHSFDPLIDSSDMQPERWIQIAEVIEENYGKYDGFIVLHGTDTMAYTASA
                     LSYMFQNL"
BASE COUNT          433 a          231 c          294 g          419 t
ORIGIN      
        1 catgttgtga tgcaggtcaa caatataatc atagccaacc atttttagct gcctgataat
       61 ttcaggaagg ttattgtcaa cataaaaagt attgtcaatg taggggttgt ttgccaatac
      121 atcactaaaa tcgcgcttgg taagaaaatg cacttctgca ttgggcaatt gttttttaat
      181 acaacgcaaa acaggtgttg ttaaaacaat atcgccaata gagctaaaac ggatgactaa
      241 aactcgggac atacagtggt tttcgaaaaa atgtctaatg ccattgtttg caaaggtaag
      301 cattggttta aatacttaaa ttcgatttac tttgcatcat gatttttaca gatacacaca
      361 cacatctcta tgccactgag tttgatgcag acaggaaaga gcttatgcaa caggctattg
      421 atgccggtgt gagtcgtttt tttcttccta atgttgaaaa atcaagtgtt aacgaaatgc
      481 ttgaattgtg caaacagttt ccggagaatt gttttcctat gatggggttg catccatgct
      541 ctgtaaaaga agattatttg gctgagttgg ctttggttga gtctgaattg tcaaagggaa
      601 aatattttgg tgtaggagaa attggtcttg acttttactg ggannnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttg tgaattaaat ctgccggtaa
      721 gcattcattc gcgctcagca actacacgtg ccattgaact tatacaatta tcaggtctaa
      781 gtgtaaaagg tgttttccat tgtttcagtg gaagtataga agaagcaaaa gctataaccg
      841 acatgggaat gtatctgggc attggaggtg tagttactta caagaattca ggtcttgatg
      901 caattgtcag tcaggtagga ttaaagcaca ttgttttgga gaccgatgcg ccatatttac
      961 ctccggtgcc tcatcgtggc aaaaggaata ttcctgaata tttactgctg acagcacaga
     1021 aattagctgt cataaaaaat tgttcggtag cagaagttgc tgaacagact acaaacaatt
     1081 ctaaagttat tttcaaacaa tgataaagac agataagaag aaccttctgg taatttatac
     1141 cggtggaacc ataggtatga ttcatgacga agagacagga tcgttaaaac cctttaactt
     1201 cggcaagctc agcaccgttg tacctgagtt gaagaaactt ccctgtaaaa tagactttca
     1261 ttcattcgac ccgctgattg acagctctga tatgcaacct gaacgatgga tacaaattgc
     1321 agaagttatt gaagagaatt atggcaagta tgatggattt attgttttac atggaaccga
     1381 tactatggca tatactgctt cggcattgag ctacatgttt cagaatctga
//
LOCUS       DAORQT010000356         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_19975_length_1418_cov_3.245048, whole genome shotgun sequence.
ACCESSION   DAORQT010000356 DAORQT010000000
VERSION     DAORQT010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19975_length_1418_cov_3.245048"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1418
                     /locus_tag="PK134_08910"
     CDS             <1..>1418
                     /locus_tag="PK134_08910"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HRB39009.1"
                     /translation="QKQVVFIFDEAHRSQFGEAQKNLNKKFKKFYQFGFTGTPIFPQN
                     ALGAETTASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSIETEIDEKKLSAA
                     ENKKALLHPERIKEISQYILQNFRIKTHRNQGTNKGFNAMFAVSSVDAAKCYYEELNN
                     LQKDSEKPLKIATIFSFAANEEQSAIGEIVDETFEPSAMDSSAKEFLTKAINDYNALF
                     KTNYGVDSNEFQNYYRDLAKRVKSKEVDLIIVVGMFLTGFDAPTLNTLFVDKNLRYHG
                     LIQAFSRTNRIYDATKTFGNIVTFRDLEQATIDAITLFGDKNTKNVVLEKSYKEYLEG
                     FTDIATGEARRGFVEVVKDLNEKFPNPDEIETEKDKKEFAKLFGEYLRVENILQNYDE
                     FSNLKAFQAIDSNDTAAVEEFKKIHFVTDEDIAVMHKIELLKDRTIQDYRSTYNDIRD
                     WLRREKAGKETAESTIDWDDVV"
BASE COUNT          523 a          242 c          292 g          361 t
ORIGIN      
        1 atcaaaagca agtggttttc atatttgacg aagcacaccg ttcacaattt ggtgaagcac
       61 aaaagaacct gaataagaaa tttaaaaagt tttatcaatt tggttttaca ggaacaccca
      121 tctttccgca aaatgcttta ggtgcagaaa ccacagccag tgtatttggt cgtgaattgc
      181 actcttatgt cattaccgat gccatcagag atgaaaaagt actgaaattc aaagtggatt
      241 ataatgatgt tcgtccacag ttcaaaagca tagaaaccga aattgacgaa aagaaattaa
      301 gtgctgccga aaacaaaaaa gccttgcttc accccgaacg aatcaaagaa atatcgcaat
      361 acattttaca gaatttcaga ataaaaacgc atcgaaatca aggcacaaac aaaggcttta
      421 atgccatgtt tgctgtgagc agtgtagatg cagccaaatg ttattatgag gaattgaaca
      481 acttgcaaaa agacagcgaa aagcctttaa agattgccac catcttttcc tttgcagcca
      541 acgaagaaca aagcgccatt ggggaaatag tagatgaaac ctttgagcca tcggcaatgg
      601 acagtagtgc caaagagttt ttaaccaaag ctatcaatga ttataacgca ctattcaaaa
      661 ctaattatgg ggttgatagc aatgagtttc aaaattatta ccgagacctt gccaaaagag
      721 taaagagcaa agaagtggat ttaatcatcg tggttggaat gttcctcacg ggttttgatg
      781 cgcctacctt gaacactttg tttgtagata agaatttgcg ttatcacggt ttgatacaag
      841 ccttctcacg aactaaccgt atttatgacg ccacaaagac ttttggaaac attgtaacat
      901 tccgtgattt ggaacaggca actattgatg ccattacttt attcggagat aagaacacca
      961 aaaatgttgt acttgaaaaa agctacaaag aatatttgga agggtttaca gacattgcca
     1021 caggtgaagc tcgcagaggt tttgtagaag tagtaaaaga cctgaatgaa aagttcccca
     1081 atcccgatga aatagaaaca gagaaagaca aaaaggaatt tgcaaaacta ttcggggaat
     1141 atttgagagt agaaaacata ctgcagaatt acgatgaatt tagtaacctg aaagcatttc
     1201 aggcaataga cagcaatgat acggcagccg ttgaggagtt taagaaaata cattttgtta
     1261 cagacgagga tatagctgta atgcataaga ttgaattgct aaaggataga acgattcagg
     1321 attaccgttc aacttataat gatattcggg actggttaag aagagaaaaa gcaggcaaag
     1381 aaacagcaga atcaaccatt gactgggatg atgtcgtg
//
LOCUS       DAORQT010000357         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_20046_length_1414_cov_6.863870, whole genome shotgun sequence.
ACCESSION   DAORQT010000357 DAORQT010000000
VERSION     DAORQT010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20046_length_1414_cov_6.863870"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..>1414
                     /gene="pnp"
                     /locus_tag="PK134_08915"
     CDS             72..>1414
                     /gene="pnp"
                     /locus_tag="PK134_08915"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRB39010.1"
                     /translation="MNLGVKHTFTLPDGREISIETGKLAKQADGSVVVRMGDCMLLAT
                     ACAKQDAMPGVDFMPLTVDYQEKYASVGRFPGGFFKREARPSEYEILISRLIDRALRP
                     MFPENFHADTQVLVTLHSGDSTVYPDALAAFAASAALTISDIPFNGPISEVRVAQIDG
                     AMVINPYIADIARATLDLMENIVMVEGEMSEVSEATMLEAIKVAHEAIKIQCKAQLDL
                     LEKIGGKTKREYNHEEHDEDLRKVVRDETYQKVYQVALQGNPNKNERKKSFEAIVEEF
                     KTRFTEEELEEKSAFINHYYHEVEYDAVREALLAEKKRLDGRGLTEVRPIWSETNYLP
                     TAHGSAVFTRGETQSLTSVTLGGKMEEQIIDGAMIEGTKKFLLHYNFPPFSTGEARPM
                     RGTGRREVGHGNLALRALTKALPPESENPYTIRVVSDILESNGSSSMATVCAGSMA"
BASE COUNT          466 a          247 c          315 g          386 t
ORIGIN      
        1 attgaataca ataatatttc agaaaggcaa tggtaaacaa ttgccttttt gtttttaaat
       61 aagacgataa aatgaattta ggagttaaac acacatttac tttacccgat ggtagagaga
      121 tttcaatcga aacaggcaaa ctagccaaac aagctgatgg ttcagtagta gtaagaatgg
      181 gcgattgtat gcttttagct actgcatgtg ccaagcaaga tgcaatgcca ggagttgact
      241 ttatgccact tacggttgac tatcaggaaa aatatgccag tgttggtcgc tttccgggag
      301 gattttttaa gcgcgaagcg cgaccatcag aatatgaaat tcttatcagc agactcattg
      361 accgtgcact gcgtccaatg tttcctgaaa attttcatgc cgacacacaa gtattagtaa
      421 cactacattc aggtgatagt acagtttatc ctgatgccct tgcagccttt gcagcatctg
      481 ctgcattaac cataagtgat attccattta acggacctat ttccgaagtt cgtgtagctc
      541 aaattgatgg tgcaatggtt atcaatccct atattgcaga cattgcacgt gccacacttg
      601 atttgatgga aaatattgtg atggtagaag gtgagatgag tgaagtaagt gaagctacca
      661 tgcttgaagc tatcaaagta gcgcatgaag caattaaaat tcagtgcaag gcacagttgg
      721 atttactcga aaaaattggt ggaaaaacca agcgtgaata caatcacgaa gagcatgacg
      781 aagatttgag aaaagtggtt cgtgatgaaa cttatcagaa agtttatcag gtagctttac
      841 aaggtaatcc taataaaaac gaaagaaaaa aatcatttga agcaatcgta gaagagttta
      901 aaacccgttt tacggaggaa gaattagaag aaaagtcagc attcataaat cattactatc
      961 atgaagtgga atatgatgca gttcgcgaag ctttattggc agagaaaaaa cgtcttgatg
     1021 gccgtgggtt aacagaagtt cgtccgattt ggagtgaaac aaattatcta ccaactgcac
     1081 atggctcagc tgtatttaca cgtggcgaaa cacaatcttt gacttctgtt acgcttggcg
     1141 gtaaaatgga agagcagata attgatggtg ccatgattga aggaactaaa aagtttttgt
     1201 tgcattataa ctttcctcca ttttctactg gcgaggcaag gccaatgcgc ggaacaggcc
     1261 gcagagaagt tggacatggt aaccttgcat tgagagcatt aacaaaagca cttccaccgg
     1321 aatcagaaaa tccatatacc atcagagtgg tttcagatat tcttgaatct aacgggtcaa
     1381 gttctatggc aactgtgtgt gcaggaagta tggc
//
LOCUS       DAORQT010000358         1386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_20676_length_1386_cov_3.933133, whole genome shotgun sequence.
ACCESSION   DAORQT010000358 DAORQT010000000
VERSION     DAORQT010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20676_length_1386_cov_3.933133"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>630
                     /locus_tag="PK134_08920"
     CDS             <1..>630
                     /locus_tag="PK134_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB39011.1"
                     /translation="PCPSFPLVTCNNGPNGDMWMNYMDYCYDKCLNMFTAGQKSRMQI
                     AMANGTYRAPLATSLGCSAPSAGPTAQFTANTTTIAPGGSVNFTDQSSNSPTSWSWSF
                     PGGTPSSSTAQNPSNIVYATAGTYNVSLTATNSNGSNTLTKNNYIVVTAGGTSTCDTI
                     SNFDLVGDTATVYLAGTTPANGYVSGQNTYSDQAKADKYSNTTPNTTVD"
     assembly_gap    631..648
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <649..1323
                     /locus_tag="PK134_08925"
     CDS             <649..1323
                     /locus_tag="PK134_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRB39012.1"
                     /translation="LIYFGVGTSSGTGQTASARVWDATGTGGMPGTAIGSVNVTYDQI
                     AADAAGTLPTIIDFNPNVSIGVGNYYVGVHFTYNAGDTLAIYTSRDGNTIPGTAYEQF
                     STGTWYAYSDATNSWDLNVAHAIFPIVCTSTGIREVMTPSNNLVVFPNPSQGEFTLIV
                     PQSDLKENVLVRVIDVKGAVVMHKELTPSGNGTYRLKLDAPAKGIYMLEVQTVNGLKK
                     QRISVN"
BASE COUNT          454 a          271 c          267 g          376 t
ORIGIN      
        1 atccatgtcc ttcttttcca ttagtaacat gtaataatgg tccaaatgga gatatgtgga
       61 tgaactatat ggactactgt tatgacaaat gtctgaatat gtttactgca ggacaaaaat
      121 caagaatgca aattgcaatg gctaacggaa cataccgtgc acccttagca acatcactag
      181 gttgcagcgc accttcagct ggaccaacag cacaatttac tgcaaataca acaactattg
      241 ctcccggtgg cagtgtaaac tttactgatc aaagcagcaa ttcaccaaca tcgtggtcat
      301 ggtcattccc aggtggaact ccatcatcct caacagcaca aaatccaagt aatatagttt
      361 atgcgactgc aggaacttat aatgtttctt taactgcaac taattcaaat ggaagcaata
      421 cacttacaaa gaacaactac attgtagtta cagcaggtgg tacttctaca tgcgacacta
      481 tttcaaattt tgacttagta ggcgatacag ctactgttta tttagccggc actacaccag
      541 caaatggata tgtttcagga caaaacacct attcagatca agcaaaagca gacaaatact
      601 ccaacacaac acctaataca actgtagatg nnnnnnnnnn nnnnnnnntt gatttacttt
      661 ggtgttggta catcatcagg tacaggacaa acggcaagtg ctcgagtttg ggatgcaacc
      721 ggaaccggtg gcatgcccgg aactgcaatt ggaagcgtaa atgttactta cgatcagatt
      781 gctgcagatg cagcaggtac tttaccaact attattgatt ttaatccgaa tgtaagtatt
      841 ggcgttggta attattacgt tggtgtacat ttcacatata atgcaggtga taccttagct
      901 atttacacca gcagagatgg aaataccatt ccgggcacag cttatgaaca atttagtaca
      961 ggcacttggt atgcttattc tgacgccact aactcatggg atttaaatgt agcacatgca
     1021 atatttccga tagtatgtac ttcaacagga ataagagaag ttatgacacc gagcaacaat
     1081 ttagtggtgt tcccaaatcc atcacaagga gaatttacat tgattgtacc gcaaagcgac
     1141 ctgaaagaaa atgttttggt acgtgtaatt gatgttaaag gtgctgttgt gatgcacaaa
     1201 gaattaacac catccggaaa cggaacctat cgtttaaaac ttgatgctcc ggctaaaggc
     1261 atttacatgc ttgaggtgca gacagtaaac ggtttgaaaa aacaacgtat atctgtgaat
     1321 taaaaaacat aagtaatgat aaaaaaagct gcttcacaag agcagctttt tttattgctg
     1381 cttgat
//
LOCUS       DAORQT010000359         1380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_20809_length_1380_cov_4.282264, whole genome shotgun sequence.
ACCESSION   DAORQT010000359 DAORQT010000000
VERSION     DAORQT010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20809_length_1380_cov_4.282264"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK134_08930"
     CDS             <1..814
                     /locus_tag="PK134_08930"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRB39013.1"
                     /translation="ISMRFGYRGPNYTTVSACASSTNALIDAFTYLRLGKADIIISGG
                     SEASVNEAGIGGFNAMHALSERNDSPQTASRPFDLDRDGFVLGEGAGCIVLEELSHAQ
                     ARGAKIYAEMVGGGMSADAYHLTATHPEGLGAFNVMKSSLDDAGMTSADIDYINVHGT
                     STPVGDPSEIKAILKLFGDDAFKLNISSTKSMTGHLLGAAGAIEAIASILAVQNNIVP
                     PTINHFTDDPTFDPRLNFTFHKPQQRNIRAALSNTFGFGGHNASVIFKKFTP"
     gene            821..>1380
                     /locus_tag="PK134_08935"
     CDS             821..>1380
                     /locus_tag="PK134_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRB39014.1"
                     /translation="MRFYGFFPINILFSADRKTYFSLHNILGFYPIKLSLYKLAFSHR
                     SQTKEVTNGIKHSNERLEYLGDAVLGAVVAEMLFKKFPFREEGFLTEMRSKIVSRENL
                     KHLAFKLGINHFIKKTHGPDSFRNIYGDALEALIGAIYIDRGYGKARQFILERMINHH
                     VDLESLEATEINFKSRILNWSQRTKNT"
BASE COUNT          411 a          293 c          271 g          405 t
ORIGIN      
        1 tatctctatg cgttttggct atcgtggccc taactacaca acggtttctg catgtgcatc
       61 atcaaccaat gcactcatag atgcatttac ttatctgcgt ttaggcaaag ccgatattat
      121 tatttcaggt ggctctgaag cttctgttaa cgaagcaggc attggaggtt tcaatgccat
      181 gcatgcactt agcgagagaa acgactcacc acaaactgcc tcacgtcctt ttgatttaga
      241 tcgtgatgga tttgtgcttg gtgaaggtgc aggttgtatt gttcttgaag agcttagtca
      301 tgcacaggct cgcggagcaa aaatttatgc tgaaatggtt ggcggtggca tgagtgccga
      361 tgcttatcat cttacagcaa cacatcctga aggtcttggt gcttttaacg taatgaaatc
      421 ttcgcttgac gatgcaggaa tgacctctgc cgacattgac tacattaatg ttcatggtac
      481 aagcacacct gtgggagacc caagtgagat taaagccata ctaaaacttt ttggtgacga
      541 tgcatttaag ctgaacatca gctcaaccaa atctatgaca ggacacctgt taggtgctgc
      601 cggagctatt gaagcaattg catctatttt agcagtacaa aataatattg taccaccaac
      661 tatcaaccat tttactgacg atcctacttt tgatccacgg ttgaatttta ctttccataa
      721 accccaacaa cgcaacatca gagcagcgct gagcaataca ttcggctttg gcggacacaa
      781 tgcctctgta attttcaaaa aattcactcc ctgataatca gtgagatttt acggcttctt
      841 tcctatcaac attttatttt cagccgatcg caaaacatac ttttcgctgc ataatattct
      901 tggcttttat ccaataaagt tatcgttata caagttagcc tttagccatc gttcgcaaac
      961 taaagaagtt accaatggca taaaacattc aaacgagcga ttagaatatc ttggtgatgc
     1021 tgtgcttggt gccgttgtag ccgaaatgct ttttaaaaaa tttccgttcc gcgaagaagg
     1081 ttttttaact gaaatgcgaa gcaagattgt aagccgcgaa aatttaaaac atcttgcctt
     1141 caaacttggt ataaatcatt ttataaaaaa aacacacggc cccgattcgt ttcgcaacat
     1201 ttatggcgat gcattagaag cattgattgg tgctatttat atagacagag gctacggcaa
     1261 agcccgtcag tttattctcg aaagaatgat taaccaccat gttgatctgg aatcgctgga
     1321 agcaactgaa ataaatttta aaagccgcat actcaactgg tcgcagcgaa caaaaaacac
//
LOCUS       DAORQT010000360         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_20964_length_1373_cov_3.753414, whole genome shotgun sequence.
ACCESSION   DAORQT010000360 DAORQT010000000
VERSION     DAORQT010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20964_length_1373_cov_3.753414"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1224)
                     /gene="sucC"
                     /locus_tag="PK134_08940"
     CDS             complement(34..1224)
                     /gene="sucC"
                     /locus_tag="PK134_08940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRB39015.1"
                     /translation="MNIHEYQGKSILKSFGVAIQEGIVAETADQAVVAAKELQNQTGT
                     NFWVVKAQIHAGGRGKGGGVKLAKSIDEVKEKANAIIGMQLVTPQTGPQGKKVHKVLI
                     AQDVYYPGETKTAEFYMSVLLNRQAGKNIIMYSTEGGMDIEEVAAKTPEKIFKEEIDP
                     AVGLQPFQARKIAFNLGVSGNAYKEMVRFVTALYKAYESCDASLFEINPVLKTSDNKI
                     IAVDAKVNLDENALFRHADYAAMRDKSEEDATEVEASEHDLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLAGGEPANFLDIGGTANAARVEQAFRIILKDPNVKAILVNIFGGIVRC
                     DRVAQGIVDAYNNIGQIPVPIIVRLQGTNAEEAKKIIDQSGLKVFSAILLKEAAQLVK
                     EKLG"
BASE COUNT          381 a          310 c          212 g          470 t
ORIGIN      
        1 attgtgcttg gttgacaggg ctttcatatt ctattatcca agcttttctt ttaccaattg
       61 tgcagcctcc tttaataaaa ttgctgagaa cacttttagt cccgactgat caattatctt
      121 ttttgcttct tcggcattgg tgccctgcaa gcgaacaata atcggaacag gaatctgtcc
      181 tatattatta taagcgtcaa caattccctg agccactcta tcgcatctca caatacctcc
      241 aaaaatattt accaatatag cttttacatt tgggtctttc agaatgatac ggaatgcctg
      301 ttctacacgg gctgcatttg cagttcctcc aatatcaaga aagtttgccg gttcgccacc
      361 tgctaattta ataatgtcca tagttgccat ggcaaggcct gcaccattca ccatacagcc
      421 tacattacca tctaacttta cataatttaa atcatgctcg ctggcttcaa cttctgttgc
      481 atcttcttct gacttatcgc gcatggcagc ataatctgca tgacggaaca aagcattttc
      541 atcgaggtta actttggcgt caacagctat aattttattg tccgatgttt ttaatacagg
      601 attaatttca aacaacgatg catcacagct ttcatacgct ttatataatg cagtaacaaa
      661 acgcaccatc tctttataag cattaccact tacaccaagg ttgaatgcaa tttttcttgc
      721 ctgaaaaggc tgaagtccta ctgcaggatc aatttcttct ttgaatattt tctcaggtgt
      781 ttttgctgcc acctcttcaa tatccatccc tccttcagtg gagtacatga ttatgttttt
      841 acctgcctga cgatttagta aaacactcat ataaaactct gcggtttttg tttcaccggg
      901 ataataaaca tcctgtgcaa ttagtacctt atgcactttt ttaccttgcg gaccggtttg
      961 tggtgtcacc aactgcatgc cgataattgc attggctttt tcttttactt catctattga
     1021 cttggctagc tttacacctc cacctttacc acgaccacct gcatgtatct gtgctttaac
     1081 tacccaaaag tttgttcctg tttgattttg aagttcttta gctgctacta ctgcctgatc
     1141 ggctgtttct gctacaattc cttcctgaat ggctacacca aaagatttta aaatggattt
     1201 gccctgatat tcgtgaatgt tcattatgaa aaattttcac aaaaataaac ttaaacatca
     1261 aaatgatgcc ttgttgttaa ataaaaagat ttgaacttat gaaatcagcc gttatattgg
     1321 cttacaaaaa aatagataaa aatcgcaatc aggattatgc ttaaccccaa ttc
//
LOCUS       DAORQT010000361         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_21249_length_1360_cov_10.311877, whole genome shotgun
            sequence.
ACCESSION   DAORQT010000361 DAORQT010000000
VERSION     DAORQT010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21249_length_1360_cov_10.311877"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..310
                     /locus_tag="PK134_08945"
     CDS             71..310
                     /locus_tag="PK134_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRB39016.1"
                     /translation="MNWQEYIMSDEEVLLGKPTIKGTRISVEHIVGLLAQGWTEKEIL
                     ENFPRLTKESLQAVFLYIQDCFSDGLLFTPKRKTA"
     gene            320..673
                     /locus_tag="PK134_08950"
     CDS             320..673
                     /locus_tag="PK134_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRB39017.1"
                     /translation="MKLLANENFPLKSIVYLQNGGFDITSIGAEHSSIKDFEVMTIAI
                     NEERTILTFDRDYGELIFKHNYKPPKGVIYLRLEKYSADEPGRLIEELVSSNQYIFDG
                     ALTVLDERGIRQRKY"
     gene            complement(810..>1360)
                     /locus_tag="PK134_08955"
     CDS             complement(810..>1360)
                     /locus_tag="PK134_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRB39018.1"
                     /translation="EKMLHDNGINDVKVISVEGQLTDHYNPLNKTVNLSQPVYYGRNA
                     AAAAVAAHECGHAVQHAKAYTMLQVRSALVPVVSFASKWVQWVLLGGVLLINVFPSLL
                     LAGIVLFAMTVLFSFITLPVEFDASRRALVWLSDSRMVTSSEHAKAKDALWWAAMTYV
                     VAALGALATLMYYVLIYFGRRN"
BASE COUNT          465 a          230 c          256 g          409 t
ORIGIN      
        1 ctattgctaa gggatatagt gattttttaa aactcaatta ttaaacagaa aaaatagtat
       61 atttgaattc atgaattggc aggaatacat aatgtctgat gaggaagtac ttttagggaa
      121 accaaccata aaaggtacaa gaatttcagt tgaacatatt gttgggctat tagctcaggg
      181 ttggacagaa aaagaaattt tggaaaattt tcctcgattg acaaaagaat ctttgcaggc
      241 agttttttta tatattcaag actgttttag tgacggatta ttatttactc ctaaaagaaa
      301 aacggcttga atttaattta tgaaacttct agctaatgaa aattttccat taaaaagtat
      361 tgtttacctt caaaacggag gatttgatat tacttccatt ggggctgaac actcaagcat
      421 taaagatttt gaggtgatga caatcgccat taatgaggag aggactatat tgacttttga
      481 tagagattac ggagaattga tttttaaaca caattacaaa ccacctaaag gtgttatata
      541 tttaaggtta gaaaaatata gtgccgatga acccgggcgt ctgattgaag aattggtaag
      601 cagtaatcaa tatatttttg atggtgcatt aacagtattg gatgaacggg gaattaggca
      661 gcgcaaatat tagtagtttg ttaaattgta atatttttaa agtaatttat tctgcattgt
      721 atcttgtaaa ttacaaattg gattcatcaa agcatacaaa aaaaagccac cccaaaaagg
      781 atggcttttc tttcttaaaa attatttttt tagttgcgtc ttccgaagta aatcaagaca
      841 taatacatca gcgttgctaa ggcacctaat gcagcaacaa cataggtcat ggcagcccac
      901 cacaaagcat cttttgcttt tgcatgttcg ctgcttgtta ccatgcggct gtcgctaagc
      961 cataccaatg ctctgcggct tgcatcaaac tcaacaggaa gtgtaataaa actaaacaac
     1021 actgtcatgg caaacaacac aatacctgca agtagtagcg atggaaaaac attgatgagt
     1081 aaaacaccgc caagcaatac ccactgtacc cattttgagg caaaacttac taccggcact
     1141 aaagccgaac gaacctgaag catggtataa gcttttgcat gttgcacagc atgaccacat
     1201 tcgtgtgctg caacggcagc agcagcagca ttacgtccat aatatacagg ctgactcaga
     1261 ttaacggttt tgttaagtgg attgtagtgg tctgtcaact gaccttcaac agaaataact
     1321 tttacatcat taataccgtt gtcatgaagc attttttctg
//
LOCUS       DAORQT010000362         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_21478_length_1351_cov_6.418210, whole genome shotgun sequence.
ACCESSION   DAORQT010000362 DAORQT010000000
VERSION     DAORQT010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21478_length_1351_cov_6.418210"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK134_08960"
     CDS             complement(<1..191)
                     /locus_tag="PK134_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRB39019.1"
                     /translation="MKVSLTARIFIGMLLAILLGHWYNISHTAAELDVFASRISILSD
                     IFLRLIKMIIAPLVFATLVV"
     gene            349..>1351
                     /locus_tag="PK134_08965"
     CDS             349..>1351
                     /locus_tag="PK134_08965"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRB39020.1"
                     /translation="MLHKVINVLILFVLFSGVANASDSLKHYKATRIFEPIKIDGQLT
                     ESAWTNTISFSDFVMNRPIEGSAPTQKTEVRIVYDNTAIYVGAMLYDTAPDSILKELG
                     LRDGADPSSNSGFTDINADYFRFVFDPYNTRQDAYDFGVYASGIQADSRYSDYLFDAV
                     WESSVKITDKGWCVEIKIPYSAIRFPSKPIQEWAFQITRNIRRNREFQQWSLTPSTAS
                     NAQNYWGVLDGIENIKPPLRLSLTPYISVAYEHEPVSGSKANQTYSYRGGADIKYGLD
                     ERFTLDMTLLPDFGQVQSDNKRKTLSYEEINYDENRPFFKEGTDIFSRDDLFYSRRIG
                     "
BASE COUNT          451 a          258 c          248 g          394 t
ORIGIN      
        1 actaccagcg ttgcaaaaac taatggagcg ataatcattt taatcaagcg aagaaaaata
       61 tcactcaata tgctaatcct tgaagcaaat acatcaagtt cggctgcagt gtgtgagata
      121 ttataccaat gtcccagcaa aattgccagc aacataccaa taaatatccg ggctgttaaa
      181 ctaactttca caataaaaac ttatgaggca aatctactgc tctgaattga aaaaacaagt
      241 ctgtgatttt tagagaataa attaatatta acatctttaa cgctgattaa cttttactta
      301 actcgaacca ctgtatgtag tgatagtttt gccgacaatt actcccgtat gttgcacaag
      361 gtcattaatg tccttattct atttgttctt ttttcaggtg tagctaatgc ctcagatagt
      421 cttaaacatt ataaagcaac acgcattttt gaacctataa aaattgatgg tcagttaaca
      481 gaatctgctt ggacaaacac catttcattt agcgattttg tgatgaaccg cccgattgaa
      541 ggtagtgcac cgacacaaaa aacagaagtg cgtattgttt atgacaacac tgcaatttat
      601 gtaggcgcta tgctttatga cacagcacct gacagcattt taaaagaatt aggcctacgt
      661 gatggtgcag acccttccag caacagtggt tttacagaca tcaatgcaga ttatttcaga
      721 tttgttttcg acccctacaa tacacggcaa gatgcttatg attttggagt gtatgcctca
      781 ggcattcaag ctgacagtcg ctatagtgat tatttgtttg atgctgtttg ggaaagttct
      841 gtaaaaatta cagacaaagg ttggtgtgta gagataaaaa tcccatactc tgcaattcga
      901 tttccttcga aacctattca ggaatgggct tttcagataa caagaaatat aaggcgcaac
      961 agagaatttc agcaatggag tcttacacca tcaacagcat caaatgctca aaactattgg
     1021 ggtgtactcg atggcattga aaacattaaa cctccgctca ggctttcact aacaccatat
     1081 attagtgtag cctacgaaca tgaacccgtt tccggttcca aagcaaatca gacttacagc
     1141 tatcgtggtg gagcagatat aaaatacgga ctcgatgagc gttttacatt agacatgacc
     1201 ttgcttcctg attttggaca agtacagtct gacaataaaa gaaaaacact aagctacgag
     1261 gaaataaact atgatgaaaa ccgtccattt tttaaagagg gtacagatat tttttcgaga
     1321 gacgatttat tctacagtag aagaattggt c
//
LOCUS       DAORQT010000363         1350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_21515_length_1350_cov_4.111197, whole genome shotgun sequence.
ACCESSION   DAORQT010000363 DAORQT010000000
VERSION     DAORQT010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21515_length_1350_cov_4.111197"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK134_08970"
     CDS             complement(<1..442)
                     /locus_tag="PK134_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HRB39021.1"
                     /translation="MSNEQLIYGTRAVMEAVNAGKDIEKIFIQKGINNSLVNELRQLL
                     TKNNIPFQVVPDFKLNKLTKGNHQGVVCILSSIVYQKTEDIIPLIFERGEVPLFLMID
                     RVTDVRNIGAMARTAACAGVHAIIIPDQGSAQLNADAIKTSAGAL"
     gene            complement(435..>630)
                     /locus_tag="PK134_08975"
     CDS             complement(435..>630)
                     /locus_tag="PK134_08975"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRB39022.1"
                     /translation="RTSDTEYWDYGFFKGYGSLNFSFRKMANPFTNNDYVLVRSAAYE
                     PVWYMAVDHWRQGRIIQNNE"
     assembly_gap    631..680
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<681..>1350)
                     /locus_tag="PK134_08980"
     CDS             complement(<681..>1350)
                     /locus_tag="PK134_08980"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRB39023.1"
                     /translation="TFFIKKTNNGSLIALRLLGNVNNIFQESYIETDSSLQGNNAFMF
                     YNETTDYPSFSNVLSNGSTTRIQNANAQLPLWVRCFYRNYPIAEPPFSSKPPLVFKYT
                     SDSSFQLVSDADKYYNLNVNRYGMYQLQYDTMLKSGAALFNFGGEFPEVTDVGPMVSA
                     LRYITTSNEFNQIQHADDRKEAIDQFWIDVSGSKERARFLIKIFYSRMQNANRFFTSY
                     LEGWK"
BASE COUNT          384 a          255 c          218 g          443 t
ORIGIN      
        1 gcagtgcacc tgcagatgtt ttaatagcat cggcattgag ttgtgctgat ccttgatcag
       61 gaattatgat tgcatgaact cctgcacagg cagcagtgcg tgccatagcg ccaatattgc
      121 gcacatctgt tacacgatca atcattaaaa aaagtggcac ttctcctctc tcaaaaatca
      181 atggaataat atcctctgtt ttttgataga ctattgagga caaaatacaa acaacacctt
      241 gatgatttcc ttttgtaagt ttgttaagtt tgaaatcggg tactacctga aaaggaatat
      301 tattctttgt aagtaattgc ctaagctcat tcaccaaact gttgttgata cctttttgga
      361 taaatatttt ttcaatatcc tttcctgcat tcacggcttc cataacagcg cgtgtgccgt
      421 atattagttg ttcattactc attgttctgt attattctac cttgtcgcca gtggtcaacg
      481 gccatgtacc agacaggttc ataggctgct gaacggacaa gaacataatc attatttgtg
      541 aatggattag ccattttcct gaatgaaaaa tttaagcttc catatccttt aaaaaaacca
      601 taatcccaat attcggtatc actggtgcga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn tttccatcct tcaagatatg atgtgaaaaa gcgattggca
      721 ttttgcattc gcgaataaaa tatttttatc agaaatcttg ctctctcctt gcttcctgaa
      781 acatcaatcc agaattgatc aatggcttct tttctatcgt cagcatgttg tatttgatta
      841 aactcgttgc tggttgtaat gtatcgtaaa gccgacacca ttggtcctac atctgttact
      901 tccggaaatt cacctccaaa attaaataat gcagctcctg atttcagcat ggtatcatat
      961 tgcaactgat acataccata acggtttaca ttcaggttat aatatttatc tgcatcactt
     1021 accaactgaa atgaactgtc tgatgtgtat ttaaaaacca atggcggttt actgctgaat
     1081 ggtggctctg caatcggata atttctgtaa aaacatctta cccacaatgg caattgtgca
     1141 ttggcatttt gaattcttgt tgtactaccg tttgaaagaa cattactgaa acttggatag
     1201 tctgtggttt cattataaaa cataaaagca ttattaccct gcaaactgct gtctgtttca
     1261 atataactct cctgaaaaat gttgtttaca tttccaagta aacgtaatgc aatcaatgaa
     1321 ccattgtttg ttttctttat gaaaaatgtt
//
LOCUS       DAORQT010000364         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_21565_length_1348_cov_5.225058, whole genome shotgun sequence.
ACCESSION   DAORQT010000364 DAORQT010000000
VERSION     DAORQT010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21565_length_1348_cov_5.225058"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1344)
                     /gene="ftsA"
                     /locus_tag="PK134_08985"
     CDS             complement(52..1344)
                     /gene="ftsA"
                     /locus_tag="PK134_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRB39024.1"
                     /translation="MAEQVTGNEVIVGLDIGTTKIAAIVGRRNEFGKIEIVGTGRAES
                     VGVTRGVVANIEKTVGSIKQAVAEAEHKCGGNIKSVHVGIAGQHIKSLQHRGMITRSS
                     TDDEISQRDIDALIDDMYKLAMPPGEEIIHVIPQEFIVDNEQGIKDPIGMSGIRLEAN
                     CHIITGLVTAANNIHKCVTKAGLTPADLILEPLASAEAVLTEEEKEAGIVLVDIGGGT
                     TDVAIFQDNIIRHTAVIPFGGNVITEDIKEGCHIIRNQAELLKIKFGSALSSQMKDNE
                     IVSIPGLRGREPKEISVKNLASIIQARVEEIIEQVYYEIKTSGFEKKLIAGIVVTGGG
                     AQLRHITQLMEYITGMDTRLGYPNEHLAKSAEETKSPIYATGVGLVIKGFQDLDKHRK
                     SGQKNTSEPRVQHKGSWFSGIFDSTKKIFTDDSDDKII"
BASE COUNT          350 a          298 c          221 g          479 t
ORIGIN      
        1 tcatagttca tcatcttaat ttattttttg ttggttatga aaatttttaa cttaaattat
       61 tttatcgtca gagtcgtcag taaatatttt tttggtagaa tcaaaaattc cgctgaacca
      121 agatccttta tgttgtacac gtggttcaga agtgtttttt tgtcctgatt ttctgtgttt
      181 atccaaatcc tgaaaacctt taatcactaa tcctacaccg gttgcataaa tcgggctttt
      241 ggtttcttcg gcactttttg ccaagtgctc gtttggatag cccaatcgtg tatccatgcc
      301 ggtaatatat tccatcaact gtgtgatgtg tcgtagttgt gcaccaccac cggtgacaac
      361 gatacctgca attagttttt tctcgaaacc ggaagttttg atttcataat aaacctgctc
      421 aataatttct tcaacacgtg cctgaataat gcttgcaaga ttttttacag aaatttcttt
      481 tggttcacga ccgcgtaatc cgggtattga aacaatctca ttgtctttca tctgacttga
      541 taatgcagag ccaaatttta tttttaataa ttctgcctga ttgcggataa tgtggcaacc
      601 ttcttttata tcttctgtaa ttacatttcc accaaatgga attacagcag tgtgccgaat
      661 aatgttgtcc tgaaaaatgg caacatctgt agttccacca ccaatatcaa ctaacacaat
      721 acctgcttct ttttcttctt cggtgagaac agcctctgca cttgccaaag gttcaagaat
      781 taaatctgca ggtgtgagtc ctgctttagt cacacacttg tggatattgt ttgctgctgt
      841 aacaagtcct gtaatgatat gacagttggc ttcaagtctg atgcctgaca tacctatcgg
      901 atcttttatt ccctgctcat tgtcaacaat aaattcctgt ggaataacat gaataatttc
      961 ttcgcccgga ggcattgcca atttatacat gtcgtcaatt aatgcatcaa tatcacgctg
     1021 gctgatttca tcatctgtgc tgctgcgtgt tatcatacct ctatgctgaa ggcttttgat
     1081 atgctgacct gcaataccaa catgcacaga ttttatattg cctccacact tatgttctgc
     1141 ttcggcaacg gcttgcttaa tactgcctac agtcttttcg atattagcaa caacacctct
     1201 tgtaacacca acagattctg ctcttccagt gcctacaatt tcaattttcc caaactcgtt
     1261 tctgcgacct acaatggctg caatttttgt agtgcctata tcaagaccta ctattacttc
     1321 attacctgtt acctgctctg ccacttat
//
LOCUS       DAORQT010000365         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_21984_length_1331_cov_4.740596, whole genome shotgun sequence.
ACCESSION   DAORQT010000365 DAORQT010000000
VERSION     DAORQT010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21984_length_1331_cov_4.740596"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK134_08990"
     CDS             <1..364
                     /locus_tag="PK134_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39025.1"
                     /translation="FDYWLTHFLYLNTSIIQPVTSKSTAIRRPAQLSFTPRLETWRYE
                     ISFPLTWYEYDQVRLGTCLRLGQVVIGSDYLTSMLGLSKFNGFDFFVGIKHSSYAMDK
                     LSKRKKEKVKDTYTGGDI"
     gene            444..>1331
                     /locus_tag="PK134_08995"
     CDS             444..>1331
                     /locus_tag="PK134_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HRB39026.1"
                     /translation="MNIKQTEQFLKKVKGMHTLVIGDVMTDAYLWGRVDRISPEAPVP
                     VVAVQKRESRPGGAANVAVNLHSLGAIPFICSVVGKDNAGKEFREMITKSGMPADGIL
                     ASSKRTTTIKTRVIGNNYQLLRVDAEVAEPLSQADGEMLIKKAIEIIRKKKPAVIVFE
                     DYDKGVLNKTVIEAIVAEAQKQNIPTTVDPKKNNFFHYRNVTLFKPNLKELREGLKTD
                     LEKPTATALTKLASDFATKQKIKSLLITLSEHGVYYYSKNEKGIIPAHFRNISDVSGA
                     GDTVISVASLAIAAGFSCHE"
BASE COUNT          435 a          240 c          277 g          379 t
ORIGIN      
        1 gtttgattac tggctgactc attttcttta tctgaacacc tcaataattc aacctgtcac
       61 cagtaagtca acagctatca gacgaccggc acaattgtct tttacacctc gattagaaac
      121 atggcgttat gaaatttctt ttcctctaac atggtatgag tatgatcagg tcagacttgg
      181 aacatgtctt cgtttaggtc aggtagttat aggttccgat tatcttactt ccatgctggg
      241 tttgtctaaa tttaatgggt tcgacttttt tgttggaata aaacacagca gttatgccat
      301 ggataagttg agtaagcgta aaaaagaaaa agttaaagac acttataccg gaggcgacat
      361 ttaaaaacta cccattgctg ccattttaat cgtttaagaa aagtagttca cattacttac
      421 ttttgccctt caattcaata ggcgtgaaca ttaaacagac agaacagttt ctgaaaaagg
      481 ttaagggaat gcatacctta gtaataggtg atgtaatgac tgatgcttat ctgtggggtc
      541 gtgttgaccg tatatcgcca gaagcacctg tgcctgttgt ggctgttcaa aagcgcgaaa
      601 gccggcccgg aggagctgcc aacgttgcag taaaccttca ttctttgggt gcaatacctt
      661 ttatatgttc tgttgttggg aaggataatg caggaaaaga attccgcgaa atgattacca
      721 aaagtggtat gcctgcagat ggaatattag catcttcaaa aaggactaca accataaaga
      781 ccagagttat aggcaacaac tatcagctat tgcgtgtaga tgcagaagta gccgagccac
      841 tgtcgcaagc agacggtgaa atgctcataa aaaaagcaat agagataatc agaaaaaaaa
      901 agccggccgt tatagttttt gaagattatg ataaaggtgt tcttaataaa actgtaatag
      961 aagccattgt tgctgaagca cagaagcaaa atattcccac aacggttgac cccaaaaaaa
     1021 ataatttttt ccattatcgt aatgtaactc ttttcaagcc taatctaaaa gagctgcgcg
     1081 aaggactaaa gactgatttg gagaaaccaa cggcaacagc gcttacgaag ttggcatctg
     1141 attttgcaac taagcaaaaa attaagtcat tgctgattac gttatcagaa catggagtat
     1201 attattattc aaagaatgaa aaaggaatta tccctgcaca cttccgaaat atcagtgatg
     1261 ttagtggtgc aggcgataca gtaatcagtg ttgcatcttt agcaattgca gcagggtttt
     1321 catgtcatga a
//
LOCUS       DAORQT010000366         1327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_22091_length_1327_cov_3.775157, whole genome shotgun sequence.
ACCESSION   DAORQT010000366 DAORQT010000000
VERSION     DAORQT010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22091_length_1327_cov_3.775157"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK134_09000"
     CDS             complement(<1..289)
                     /locus_tag="PK134_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRB39027.1"
                     /translation="MEKKRTYLGNADPVAIEALYNSYLKDPQSVDQTWQTFFDGFELA
                     RKSQSPDGGNVVSAADASHIEKEFMVLRLISDYRKRGHLFTDTNPVRERRKY"
     gene            complement(389..1108)
                     /gene="recO"
                     /locus_tag="PK134_09005"
     CDS             complement(389..1108)
                     /gene="recO"
                     /locus_tag="PK134_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRB39028.1"
                     /translation="MSALHNTDGIMLHLSPYGDSSVIAKIYTEKFGIQTYLVNGVRSS
                     KSKNKSVFFQPLSLLQLVVYHRPDKGLQRISNLAFANRFISIPFNITKSTQSIFMAEL
                     LYRTIREEEANPALFRFLFDNIVQLDKSLSANPDFHLLFMIKLSAFLGFRPTDNYEEH
                     LCFDMKEGCFSTKNSSHLYVIDESTSLLLHQCLNSSIKEPQLNYKQRTDLLNVLLQYY
                     QLHLPDGFRLQSMDILTELFN"
BASE COUNT          453 a          221 c          239 g          414 t
ORIGIN      
        1 gatattttct gcgctcgcga acaggattgg tatccgtaaa aagatgtcca cgtttgcgat
       61 aatcgctgat taatcgcaat accataaact ctttttctat atgtgatgca tcggctgcag
      121 aaacaacatt tccaccgtca ggagactgac tttttcttgc cagttcaaat ccatcaaaaa
      181 aagtttgcca ggtttgatct accgactgtg ggtctttaag atatgagtta taaagcgctt
      241 ctattgctac tgggtcagca ttaccgaggt aagtacgttt cttttccact ttaaaattta
      301 actgaattca gcgcacaaag gtaggcaatt tgccggtgta aaaactgatt ttagttagtt
      361 gaataaccaa tctgcgttta aaaactcatc aattaaacag ttcggttagt atatccatgc
      421 tttgtaaacg gaacccatca ggaagatgta attgataata ttgtaagaga acatttagta
      481 aatctgttcg ctgtttataa tttaactgcg gttctttgat tgaagaatta agacattgat
      541 gtaataataa cgaagttgat tcatcaataa cataaagatg tgaagaattt tttgtgctga
      601 aacagccttc cttcatatca aaacacagat gttcctcata attatctgtt ggcctgaatc
      661 caagaaaagc agatagttta atcataaata aaagatggaa atcaggatta gccgataaag
      721 atttatcaag ttgaacaatg ttatcaaaca aaaatctgaa gagagcagga ttagcttcct
      781 cttcacgaat ggtacgataa agcaactctg ccataaaaat tgactgcgtg ctttttgtaa
      841 tgttaaatgg aattgaaata aatctgttgg caaaagcgag gttacttatg cgttgcaaac
      901 ctttatccgg acgatgatat acaacaagtt gcagcaaact caacggttga aaaaacacac
      961 tcttgttttt tgattttgaa gaacgtacac cattgactaa ataggtttgt atgccaaact
     1021 tttccgtata aattttagca ataacagatg aatcgccata gggcgacagg tgcaacataa
     1081 ttccatctgt gttatgcaag gcagacattc cgctttttag tgatttgaat attttgtggc
     1141 aaatttatga caagatgatg acgattcagc ataaagataa aacagtttta tcacttcctg
     1201 aatagaatga aaaatagtat atataaatta tttacgatat ttttgtcgta aattaaaaaa
     1261 ctcactactt ttgtaccccg aaaagacgca acaatgtttt ctaaagcatg tgagcatggc
     1321 ataaaag
//
LOCUS       DAORQT010000367         1315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_22383_length_1315_cov_6.267460, whole genome shotgun sequence.
ACCESSION   DAORQT010000367 DAORQT010000000
VERSION     DAORQT010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22383_length_1315_cov_6.267460"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1076)
                     /locus_tag="PK134_09010"
     CDS             complement(105..1076)
                     /locus_tag="PK134_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB39029.1"
                     /translation="MIKILANDGIDAAGKKIFDSAGFSVSTDKVPQEKLAEALKNFDA
                     ILVRSATTVRTDVIDACPNLKVIGRAGVGMDNIDVKYARDKGLAVINTPAASSQSVAE
                     LVFAHLFTGVRMLHDANRNMPACATKETFDKLKKSYSAGIELKGKTIGIIGAGQIGKC
                     VAAIAVGLGMNVLFHRLNNEDVVVDMDFHPALGQKKITLTFKCVSFTKLLIESDFITM
                     HVPFPKGSAPVIGKKELDLMKNGVGIVNTARGGVISEADLIDALNSGKVAFAGIDVFE
                     GEPLINQTLCAHPKVTLSPHIGGSTKEAQERIGIELAEKVVEYFTKK"
     gene            complement(1095..>1315)
                     /locus_tag="PK134_09015"
     CDS             complement(1095..>1315)
                     /locus_tag="PK134_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRB39030.1"
                     /translation="APEDRSPMNACFLLKDASMDEAFLAKAKEAGIVGIKGHRSVGGF
                     RASMYNALPVESVKVLVQVMKDFAKANG"
BASE COUNT          368 a          279 c          220 g          448 t
ORIGIN      
        1 tcataagggc ggcaagcaat ccgctttact aattcttttt ttggtcttat tcctttgaat
       61 ggttttaata ttgccatacg ttttggtaaa ttttaattct gattttattt tttagtaaaa
      121 tattcaacaa ctttttcagc aagttcaatg cctatgcgct cctgtgcttc tttggttgaa
      181 ccaccaatgt gtggtgacaa agttactttg ggatgtgcgc aaagtgtttg gttaattaat
      241 ggttcgcctt caaaaacatc tattcctgca aaagctactt tccctgagtt gagtgcatca
      301 attaaatcgg cttctgaaat tacaccacca cgggcagtgt taacaatgcc tacaccattt
      361 ttcatcaggt caagttcttt cttgccgata accggtgcac ttccttttgg aaaaggcaca
      421 tgcatagtta taaaatcact ttcaataagg agttttgtaa acgaaacaca tttaaaagtg
      481 agtgttattt ttttctgtcc taaggcagga tgaaaatcca tatctactac cacatcttcg
      541 ttgttcagtc tgtgaaacaa aacattcatt cccaaaccaa cggctatggc agctacgcac
      601 ttaccaattt gtccggcacc gataatgcct attgttttcc cttttaattc tattcctgca
      661 gagtaagatt tttttagctt atcgaatgtt tctttagtag cgcaggcagg catattgcgg
      721 ttggcatcat gcagcatacg tactcctgta aataaatgtg caaaaaccaa ttccgcaacc
      781 gattgtgatg atgctgccgg ggtattgatt actgcaagtc ctttgtcgcg ggcatacttt
      841 acatcaatat tatccatacc tacaccggca cgaccaatta ctttaaggtt tggacatgca
      901 tcaataacat ctgtgcgaac agttgtagca cttcttacta atatagcatc aaaattcttt
      961 aatgcttccg caagtttttc ctgaggtact ttatctgtag aaactgaaaa tcctgctgaa
     1021 tcaaatattt tttttcctgc agcatcaatg ccatcattgg ctagtatttt aatcataact
     1081 gaatattttt ttatttaacc gttagctttt gcaaaatctt tcatcacctg caccaatact
     1141 tttacacttt caacaggaag tgcattgtac atactggcac gaaaaccacc tacactacga
     1201 tgacctttaa tacctacaat gccggcttct tttgcttttg ctaagaaagc ttcgtccata
     1261 gatgcatctt ttaatagaaa acaagcattc attggagaac gatcttccgg tgctg
//
LOCUS       DAORQT010000368         1312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_22469_length_1312_cov_5.733492, whole genome shotgun sequence.
ACCESSION   DAORQT010000368 DAORQT010000000
VERSION     DAORQT010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22469_length_1312_cov_5.733492"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>278)
                     /locus_tag="PK134_09020"
     CDS             complement(<1..>278)
                     /locus_tag="PK134_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRB39031.1"
                     /translation="GASLGVDAIKIAALDYGSALFNRIESVEIKTENQLLFETAGTPY
                     AKRIKGIVAIYERVAKAVSETLKNKDFPIVLAGDHSTAGGTVAGIKMT"
     assembly_gap    279..308
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            428..>1312
                     /locus_tag="PK134_09025"
     CDS             428..>1312
                     /locus_tag="PK134_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRB39032.1"
                     /translation="MKNRYIDLIQQSYDFPQEGFEVIDDSLHFNGINLMDVIKQYGTP
                     LKITYLPKISSQIQKAKRLFKVAMAKVDYKGSYYYCYCTKSSHFQFVMEEALKNDIHI
                     ETSSAFDIPIIRSLYESQKINKDIYILCNGFKREGYIEGIVNLIKDGFTNVIPILDNV
                     DELQEYEKAMPGKKFKIGIRIASEEEPNAEFYTSRLGIRQRDIVDFYKNTIKKNPNVE
                     LKLLHFFINTGIRDTAYYWNELQRNIAFYADLKKICPELTALDIGGGFPIKTKLEFNF
                     DYQYMADEIVMQIKSACDQ"
BASE COUNT          407 a          228 c          231 g          416 t
ORIGIN      
        1 aagtcatttt aatgcctgca acggtaccac cggcagtgct atggtctccg gctaacacaa
       61 tcgggaaatc tttatttttt aatgtttcag aaacggcctt tgccacacgt tcataaatag
      121 caacaatgcc ttttatacgt tttgcataag gagtacccgc tgtctcgaag agtaattggt
      181 tttcagtctt gatttctaca ctttctatcc tgttgaaaag tgcactgcca tagtctaagg
      241 ctgcaatttt tattgcatca acaccaaggc ttgcacctnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnntt tctgatttaa cttcaatgaa ttttatttta cttgccataa attttttaac
      361 atcgttattt tttattcagg ttatttttac attcgcaagg cacaaaaata agcctttcgt
      421 attgcaaatg aagaaccgtt acattgacct gattcaacaa agttacgact ttccacaaga
      481 aggttttgaa gttattgacg attcactcca ttttaatggt ataaacctga tggatgtgat
      541 aaaacaatat ggcacaccgt tgaaaataac ctatctgcct aagattagct cacaaattca
      601 aaaagccaaa cgacttttta aagttgctat ggcaaaagtt gactataaag gaagttatta
      661 ctactgttat tgcacaaaaa gttcgcactt tcagttcgtg atggaggaag cactgaaaaa
      721 cgatattcat attgaaacaa gttcggcttt tgatattcct attatcaggt cgctctatga
      781 gtcgcagaaa ataaacaagg acatttatat tctttgcaat ggttttaaac gcgaaggtta
      841 tattgaaggt attgttaatc ttattaaaga tggttttacc aatgtgattc caattctgga
      901 taatgttgat gaacttcagg aatacgagaa ggcaatgccc ggcaagaagt ttaaaatcgg
      961 aatcagaatt gcttctgaag aagagcctaa tgcagagttt tacacttcac gtttaggtat
     1021 caggcaacgt gacattgttg acttttacaa gaataccatc aagaagaacc ctaatgttga
     1081 actaaagctt cttcattttt ttatcaatac aggcattcgt gatactgcat attactggaa
     1141 tgaacttcag cgaaacattg cattctatgc agaccttaaa aagatttgtc ctgagttgac
     1201 agctcttgat attggtggtg ggtttccaat taaaacgaaa cttgaattta atttcgacta
     1261 tcaatatatg gctgacgaga ttgtaatgca gataaaatcg gcatgcgatc ag
//
LOCUS       DAORQT010000369         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_22902_length_1296_cov_2.536664, whole genome shotgun sequence.
ACCESSION   DAORQT010000369 DAORQT010000000
VERSION     DAORQT010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22902_length_1296_cov_2.536664"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PK134_09030"
     CDS             complement(<1..1064)
                     /locus_tag="PK134_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRB39033.1"
                     /translation="MKFENITHVYFIGIGGIGMSALARYFKAIGKQVAGYDKTQTALT
                     EELKGENIDVHFNDDVQAIPLAFMEANRKSNILVVYTPAIPKDHSELNFFSQQEFTVV
                     KRSQVLGVITSMHKTLAVAGTHGKTTTSSILAHILKTANINVAAFLGGIARNYNSNII
                     LPVSEHEVINVVEADEFDRSFLTLFPFAAAITSMDADHLDIYGSGEELKKTYQQFASQ
                     VDEKGLLVLRNGLQLQPVKALTKFYAADSQADYFADKISIRNHRYCFNFHSPDGIIED
                     LSLGLPGRHNVENAVAAIALAMKVGVKEESVRMALQSYKGVKRRFDIRFSDANCTFID
                     DYAHHPEELRAAIMSAHELF"
     gene            complement(1061..>1296)
                     /locus_tag="PK134_09035"
     CDS             complement(1061..>1296)
                     /locus_tag="PK134_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39034.1"
                     /translation="EAMVKRDAAVLVKDTDALQHLADTALNIINDADRLKQLSANAAA
                     MALPHAAQKIADEVIAMATQHAAQNLKHVTAGS"
BASE COUNT          385 a          267 c          245 g          399 t
ORIGIN      
        1 tcaaacaatt cgtgtgctga catgatagca gcgcgcagtt cttcaggatg atgtgcatag
       61 tcatcaatga aagtacaatt agcatcagag aaccggatgt cgaatctgcg ttttacacct
      121 ttataacttt gcagcgccat tcgtaccgat tcttctttaa caccaacttt cattgcaaga
      181 gcaatagctg caacagcatt ttcaacatta tgacggccgg gaagtcctaa agaaagatct
      241 tcaataatac catcaggcga atgaaaatta aaacaataac gatgattacg gattgaaatc
      301 ttatctgcaa aataatcggc ctgtgaatct gcagcataga acttcgttaa tgccttaacc
      361 ggttgtagtt gcaatccgtt tcgtaaaaca agcaatcctt tttcgtcaac ctgagaggca
      421 aactgctgat aggttttctt taactcttcg ccactgccgt aaatatcaag atggtcggca
      481 tccatacttg taatggcagc agcaaaagga aataaggtaa gaaacgaacg gtcaaattca
      541 tctgcttcaa ccacattaat aacttcatgt tcactgacag gaagaataat gttgctgtta
      601 taatttcttg ctattccccc aagaaaagca gccacattaa tatttgctgt tttaagaata
      661 tgggccaaaa tacttgaagt agtggtcttg ccatgtgtgc ctgctacggc aagtgtttta
      721 tgcattgaag tgatgacacc taaaacctgt gagcgtttta caacagtaaa ctcctgctga
      781 ctgaaaaaat taagttctga atgatcttta ggtattgcag gtgtgtaaac aaccagaata
      841 ttactctttc tgtttgcctc cataaatgct aaaggtatag cttgtacatc atcattaaaa
      901 tgcacgtcta tattttcgcc ttttaactcc tcagttaatg ctgtttgagt tttgtcataa
      961 ccggcaactt gtttgccgat agctttaaaa taacgtgcaa gcgcactcat tccaatgccg
     1021 cctatgccga taaaataaac atgtgttata ttctcaaatt tcatgagcct gcagttacat
     1081 gtttaaggtt ttgtgctgca tgttgcgtag ccattgcaat aacttcgtca gcaatttttt
     1141 gtgctgcatg aggcagtgcc attgctgcag cgttggccga aagttgtttc aaacgatcgg
     1201 catcattaat aatgttcaat gctgtatcag ctaagtgttg caatgcatcg gtatctttta
     1261 ctaaaaccgc tgcgtctctt ttcaccatgg cttcaa
//
LOCUS       DAORQT010000370         1288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23113_length_1288_cov_5.347932, whole genome shotgun sequence.
ACCESSION   DAORQT010000370 DAORQT010000000
VERSION     DAORQT010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23113_length_1288_cov_5.347932"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PK134_09040"
     CDS             <1..1167
                     /locus_tag="PK134_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39035.1"
                     /translation="PLNPINYFPQIKDSIPPLLKSITIYSIKEDFLSIFPWHEIKPVE
                     TSRGNYSYTELISASGEIGIGFSGYDQANDDNAILGIDEAELYANEKLIYARKMSNFS
                     FDETRNANGLIDYAKGQRTGSITERCYKLPGVKFSQYIFLKNSGVVDLENDSIINFRL
                     MLRDGVGNISRCVFTVAYSNNAKPKNTVVKKGELLEYNKANKIQSDRYKLMLPAGALF
                     RDFYCDAIIRRGNKSEGLKKNITLKIKSDLEGDSLNYKTIMVKIASNGTASPVSGIYK
                     DGWYSASISSFGTYSLMVDSISPVIENISTSFDSVYNCPVLEIKVSDNFSGIKNYSCM
                     INNKWHLSEYDRKNGTIYVLLNQFAESKFNIKIDLSDMCNNTTTLSKEIILSKK"
BASE COUNT          463 a          211 c          219 g          395 t
ORIGIN      
        1 cctttaaacc ctatcaacta ctttcctcag attaaagaca gcattccacc gctgcttaaa
       61 tcaattacaa tctattctat taaggaagat tttttgagca tttttccatg gcatgaaatt
      121 aaacctgttg aaacgtctcg agggaattat agctatactg aattaatatc ggcatccggt
      181 gaaattggaa taggattttc aggctatgat caggctaacg atgacaatgc aattttggga
      241 attgatgaag cagaactata tgccaatgaa aaattaattt atgctcgaaa aatgagtaac
      301 ttttcttttg atgaaacacg aaatgcaaac ggactcattg actatgcaaa aggtcagcga
      361 acaggaagca ttaccgaacg ctgttacaaa cttcctggag taaagttcag tcagtatatt
      421 tttcttaaaa atagtggtgt tgttgattta gagaatgaca gtataataaa tttcagacta
      481 atgttgcgtg acggtgtagg aaatatttca cgctgtgtct ttacagtagc ttatagcaac
      541 aatgccaagc ctaaaaatac agttgtaaaa aaaggagaat tgttggaata taataaggca
      601 aacaaaattc aatctgaccg ttataaacta atgttacctg caggagcatt atttcgtgac
      661 ttctattgtg atgccataat tcgcagagga aacaaatcag aaggattgaa aaagaatata
      721 actctaaaaa taaaatctga tcttgaaggc gattcgctca attacaaaac cataatggta
      781 aaaattgctt caaacggaac agcatcaccc gtatccggaa tttataaaga tggttggtat
      841 tcagcaagca tcagcagttt tggcacttac tcacttatgg ttgacagcat tagtcctgtc
      901 atagaaaata tttcaacttc attcgactca gtttataatt gtccggttct tgaaattaaa
      961 gtatcagaca atttttcggg aattaagaat tattcctgta tgattaacaa taaatggcac
     1021 ttatctgaat atgacagaaa gaatggcact atctatgttt tattaaacca atttgcagag
     1081 agtaaattta atattaaaat tgaccttagt gatatgtgca ataacacaac aacattgtca
     1141 aaggaaatta ttttatctaa gaaataaaat cttaaatctc gcttagctta aaagtggaat
     1201 ttacacccac acctttttct gtttccaaaa gattacaaca ctcattagtt gggtcatgct
     1261 ctaaaaacaa attaagctta ttgtgcac
//
LOCUS       DAORQT010000371         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23172_length_1286_cov_4.843217, whole genome shotgun sequence.
ACCESSION   DAORQT010000371 DAORQT010000000
VERSION     DAORQT010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23172_length_1286_cov_4.843217"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PK134_09045"
     CDS             <1..272
                     /locus_tag="PK134_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HRB39036.1"
                     /translation="VLGGVKYSIDMVSQAKVKAKDKEYVKLQRNDYGYEIGVGCDFYL
                     PMFKFSPEIKMYHGLRNLLVKDDLVYSKSLDGLFSKMFTLSLTFE"
     gene            288..>1286
                     /locus_tag="PK134_09050"
     CDS             288..>1286
                     /locus_tag="PK134_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRB39037.1"
                     /translation="MQTFELVVLPEEAASESVIQHIVAKKFGGNRLFRIIKRSIDARS
                     KTVKIRLQLQWTDEQNQETLVENRKWRQVANSEPVIIAGCGPAGLFAAIRLIECGFKP
                     IIIERGKDVRNRRRDLAAINKQRQINPESNYCFGEGGAGTYSDGKLYTRSNKRGNIAS
                     VLNTFIEHGADGDIAVDAHPHIGTNKLPAIISAMRNRIVECGGEVLFDTKLVNIDFDT
                     SGVNGVEVQQGLAQFHIKSKALILATGHSARDVFYMLNNQKILIEAKPIAIGVRAEHQ
                     QQVIDSVQYHCSKRSQFLPPASYALKHQHDGRGIYSFCMCPGGIIAPAATDIGEVVV"
BASE COUNT          415 a          204 c          285 g          382 t
ORIGIN      
        1 atgtattagg aggagttaag tacagcatag atatggtatc gcaggcgaaa gtaaaagcaa
       61 aggataaaga atatgtaaag ttgcaacgaa acgattatgg atatgaaatt ggagtaggat
      121 gtgattttta tttgcctatg tttaagtttt caccggagat aaagatgtat catggtttac
      181 gaaatctttt ggttaaagac gatttggttt actcaaaatc tcttgatgga ttgttttcaa
      241 aaatgttcac cttgtcattg acattcgaat aatagaagat actttttatg caaacatttg
      301 aacttgttgt tctgcccgaa gaggcagcca gtgagtcggt aattcaacac atagttgcta
      361 agaaatttgg tggcaacaga ttgttccgta ttataaaacg cagtattgat gcccgctcta
      421 agactgtaaa aatcagattg cagttacaat ggacagatga acagaatcaa gaaactttag
      481 ttgaaaatcg aaagtggcgg caagttgcca atagtgaacc tgtaataatt gcaggatgtg
      541 ggcctgcagg attgtttgca gccatccgac ttattgaatg tggatttaaa ccaataataa
      601 ttgaacgagg caaagacgta cgtaatcgca gacgtgacct tgctgcaata aataaacaaa
      661 ggcagattaa tccggagtca aattattgtt ttggcgaagg tggtgcaggc acctacagcg
      721 atggaaaact ttatacacgt tccaataaga gaggcaatat tgcttctgta ttaaatacat
      781 ttattgagca tggtgccgat ggtgatattg ctgttgatgc acatccacat attggaacca
      841 ataaacttcc tgcaataatt tctgcaatgc gaaacagaat tgttgaatgt ggaggcgaag
      901 tattattcga tacaaaactt gtaaatattg attttgacac aagtggtgta aatggagtag
      961 aggtgcaaca agggttggca caatttcata ttaaatctaa agctctgatt ttagcaaccg
     1021 gtcattctgc acgcgatgtt ttttacatgt taaacaatca aaaaatattg attgaagcaa
     1081 aaccaattgc tattggtgtt cgtgctgaac atcagcagca ggttattgac tctgtgcagt
     1141 atcattgcag taaacgcagt caatttttgc caccggcatc ttatgcatta aaacatcagc
     1201 atgatggtcg aggaatttat tctttctgca tgtgtccggg aggcatcatt gcacctgctg
     1261 caaccgacat tggcgaagta gttgtt
//
LOCUS       DAORQT010000372         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23288_length_1282_cov_3.063570, whole genome shotgun sequence.
ACCESSION   DAORQT010000372 DAORQT010000000
VERSION     DAORQT010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23288_length_1282_cov_3.063570"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PK134_09055"
     CDS             complement(<1..873)
                     /locus_tag="PK134_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB39038.1"
                     /translation="MKKKPPFHNLRSIFKQYNTFTYSERKGIIVLSIIIVVLLLTLYG
                     LKFYEPPIAINTSRLDSAVAQWNLLQADSIVVKDATKLSENNSPQTTRILFPFNPNNL
                     ADSLWTKLGLSEKQIASIKKYEERGGKFRIRSDVKKLFVITPSFYDSIYSFILLPDSM
                     PTFAQNKYQQDSIKVKKEWTIEPIEINLADEEEWQKLPAIGPGRAAAIVSYRNKLGGF
                     TSINQLHEVNAIVDSVFEKISPYLLCNIKPIQKKNINTDDAFSLRHPYISSSLATIIV
                     NYRKMHGDYKSTEQL"
     gene            1014..>1282
                     /locus_tag="PK134_09060"
     CDS             1014..>1282
                     /locus_tag="PK134_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HRB39039.1"
                     /translation="MSVLPPCPLCHSIYTYENGNLMVCPECFYEWNPEEQQSVSEETT
                     SADSGKVFDANGNELQNGDAVIVIKDLPVKGAPKPVKAGTKVKNIR"
BASE COUNT          398 a          205 c          248 g          431 t
ORIGIN      
        1 taattgttct gttgatttat agtcaccatg catttttctg tagtttacaa ttatagttgc
       61 cagactgctg ctgatgtatg gatgtctgag gctaaatgca tcatcggtat tgatgttctt
      121 cttttgaatg ggctttatat tacaaagtaa ataagggctg attttttcaa aaacagaatc
      181 aacaatagca ttgacttcat gcaattgatt aattgatgta aaaccaccta atttattacg
      241 atacgaaact atagctgctg ccctgccagg accaatagca ggcaacttct gccactcttc
      301 ctcatcagct aaattaattt caataggctc aatagtccat tcttttttta ctttaataga
      361 atcttgctga tatttattct gagcgaatgt cggcattgaa tcaggcagaa gaataaagga
      421 ataaatactg tcataaaacg aaggggtgat gacaaagagt ttttttacat ctgaccggat
      481 tctgaatttt cctccacgct cttcatattt tttgattgat gcaatttgtt tttctgataa
      541 acctaacttg gtccaaagcg aatcggctaa attatttgga ttgaaaggga aaagtattct
      601 agtagtttgt ggagaattat tttctgaaag tttcgttgcg tctttgacaa caatagaatc
      661 ggcctgtaac aaattccatt gtgctacagc agaatctaaa cgacttgtgt tgatggcaat
      721 tggtggctca taaaatttaa gtccgtacag tgtcagcagc agtacaacaa taataatgga
      781 taatacgata atgcccttac gttcactgta agtaaacgtg ttgtactgtt taaaaatact
      841 ccgtaggtta tgaaaaggcg gtttcttttt catggttaaa acggtaagtt aattttctaa
      901 agcgaagata gtattttaaa acagagtttg tatgcaatgg atttcatttt cttgaagcct
      961 gattgattat aatgtggggg tttttaaaga tatttgcaga aaatattttt atcatgtctg
     1021 tcttgccgcc atgtccttta tgtcattcaa tttataccta tgaaaatggt aatctgatgg
     1081 tttgtcctga atgtttttac gagtggaatc ccgaagaaca acaatctgtt tcagaagaaa
     1141 cgacaagtgc tgatagtgga aaagtttttg atgctaacgg aaatgaattg caaaatggcg
     1201 atgcagtaat tgtcattaaa gatcttcctg taaaaggagc acccaaacct gtgaaagccg
     1261 gcaccaaggt taagaacatt cg
//
LOCUS       DAORQT010000373         1273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23509_length_1273_cov_4.494253, whole genome shotgun sequence.
ACCESSION   DAORQT010000373 DAORQT010000000
VERSION     DAORQT010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23509_length_1273_cov_4.494253"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK134_09065"
     CDS             <1..533
                     /locus_tag="PK134_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39040.1"
                     /translation="ANTSSQKGWITDCKGAGQIKVSNISPFSGKQCLEMAHQNQATCT
                     FSQSIIIENTAIKNYKIIYSCRAEALNGNTGLNIIIRDASGKKLTESSGFIKNAEAKW
                     KTYSVAMAVPDGAQTMSITGILNGTGNVWFDEIKVEELKQHKPSSGIWSTASEFMTIA
                     GKYSIGEAIKWAVEKK"
     gene            complement(542..>1273)
                     /locus_tag="PK134_09070"
     CDS             complement(542..>1273)
                     /locus_tag="PK134_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB39041.1"
                     /translation="VEMQFADFVTCGFNQIVNNLAKTHYRWGQNADVVVRMPTGAAVG
                     AGPFHSQSTEAWFFHVPGLKIVYPAFPGDAKGLLAAAIEDPNPVMFFEHKALYRSVSG
                     LVADDYFTIPIGKANKIREGSDLTIVTYGLGVHWAMEALDENKNIQADLIDLRSLLPW
                     DKETVLESVKRTGKVIVFHEDTLTGGIGGEIAAEISSACFQWLDAPVMRCGSLDTAVP
                     MSNHLEWNFLPRDRFKQMLKQLAEY"
BASE COUNT          434 a          250 c          237 g          352 t
ORIGIN      
        1 atgctaatac ttcttctcag aaaggatgga taacagattg taaaggagct ggtcagataa
       61 aagtcagtaa catttctcca ttttcaggta aacaatgtct ggaaatggca catcaaaatc
      121 aggctacatg cacattcagt cagagtataa ttattgaaaa cacagcgata aaaaattaca
      181 aaataattta ttcgtgcaga gcagaagcac tgaatgggaa taccggtctt aatataatta
      241 tcagggatgc atcaggaaaa aaattaactg agagcagtgg ttttattaag aatgcagaag
      301 caaaatggaa aacttattct gttgcaatgg ctgttcctga cggagcgcaa acaatgagca
      361 tcacaggaat tttgaatggc acaggcaatg tttggtttga tgaaataaaa gtagaagaat
      421 taaaacagca taaaccctca tcaggaatct ggtcaactgc aagtgagttt atgactattg
      481 caggaaaata ttctattggc gaagcaataa aatgggcagt tgaaaaaaaa taatttacag
      541 cttaatattc agctaattgt ttgagcattt gtttaaatct atcacgcggc agaaaattcc
      601 actccaaatg atttgacata ggcacggcag tatcaagact accgcatcgc atcacaggcg
      661 catcgagcca ttgaaaacaa gcagaagaaa tttcagcagc aatttcacct cctattcctc
      721 cggttaaagt gtcttcgtga aatacaataa cttttcctgt tcgttttaca gattctaaaa
      781 cggtttcttt atcccaaggt aaaaggcttc tcaggtcaat taaatcagcc tgaatatttt
      841 tattttcatc caatgcttcc attgcccaat gtacacctaa accataagtt acaattgtta
      901 agtcagaacc ttctctgatt ttatttgcct tacctatagg aattgtaaag taatcatcag
      961 caacgaggcc gctaacactt ctataaagtg ctttatgttc aaaaaacatt acagggttcg
     1021 ggtcttcaat agcagcagcc aataaacctt ttgcatcacc cggaaatgca ggataaacga
     1081 tttttagtcc cggcacatga aaaaaccatg cttctgttga ttgcgagtga aatggtcccg
     1141 caccaacagc agcgcctgtg ggcatacgca ctactacatc tgcattttgc ccccagcgat
     1201 aatgtgtctt tgccaggttg tttacaatct gattgaaacc gcaagtaaca aaatcggcaa
     1261 actgcatttc aac
//
LOCUS       DAORQT010000374         1271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23579_length_1271_cov_3.814967, whole genome shotgun sequence.
ACCESSION   DAORQT010000374 DAORQT010000000
VERSION     DAORQT010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23579_length_1271_cov_3.814967"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK134_09075"
     CDS             complement(<1..196)
                     /locus_tag="PK134_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39042.1"
                     /translation="MIKNIKITTAFAILAITLSTVSCVKKDDFDNAPTTNVDPDIVTN
                     ATVSSVVTLANATGPVQITSD"
     gene            complement(223..>1271)
                     /locus_tag="PK134_09080"
     CDS             complement(223..>1271)
                     /locus_tag="PK134_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB39043.1"
                     /translation="TSVYREGNYKNGLFPNDSYGKSKTYVFPDVGVKAGATYKVNGRN
                     YVFANAGYMTRAPFFNNMYLSPRSRNTTVGDLTSEKITSVEGGYLHIAPKIKGKLKLY
                     YTNFKDGIESRSYYLQGFGTNSYVNYTLSGIEKVHAGTEISVDANLGMGLSAMAVASI
                     GQYYYSSRPTATITVDNTNTVQSTGEVVYMKNLHVDNTPQNAFTVGLNYRSKKFWYVG
                     VNANCFSNSYVEASPARRTGDIIDGVDENSQAWEKILGQERYDAQMTLDLSAGWSIKL
                     NNHIKALKRNTFFLINLNINNLLDNKDIITNGYEQGRLDKSAVLSGRPDVFASKYSYA
                     FGRTYMISFILRMN"
BASE COUNT          381 a          241 c          216 g          433 t
ORIGIN      
        1 aatcagaagt tatctgaacc ggccctgttg cattagctaa agttaccaca ctcgatactg
       61 ttgcattggt aacaatatcc ggatcaacat tagtggtagg tgcattgtca aaatcatcct
      121 ttttaacgca ggaaacagta cttaaagtaa ttgcaagaat tgcaaatgct gttgtgattt
      181 taatattctt tatcattgtt ttattatatt tctaattagt tattaattca ttcttaaaat
      241 aaagctgatc atatatgttc tgccaaaagc ataactatat tttgaagcaa atacatcagg
      301 tcgtcctgaa agcacagctg atttatccaa tctgccttgc tcatatccat tggtgataat
      361 gtctttatta tcgagcaggt tattgatgtt aaggttaatc aggaagaaag tatttctctt
      421 taaagctttg atatggttgt tcagtttaat tgaccaacct gcactcaggt caagagtcat
      481 ttgtgcatcg tagcgctcct gtccaagaat tttttcccat gcctgactat tttcatctac
      541 tccgtcaatg atatcacctg tgcgtcttgc aggactagct tcaacataag aattagagaa
      601 gcaattggca ttaacaccaa cataccagaa ttttttcgaa cggtaattca atccaaccgt
      661 aaatgcattt tgaggtgtgt tgtccacatg caggtttttc atgtaaacta cttcgcctgt
      721 tgattgaact gtgtttgtat tatcaacagt aatagttgct gttggacgtg agctataata
      781 atactgacca attgaagcta cagccattgc agaaagtccc attcccagat tagcatcaac
      841 agaaatttct gttcctgcat gtactttttc aattcctgaa agtgtataat tcacataact
      901 gtttgtgcca aaaccttgca gataatatga gcggctttca ataccgtctt tgaagtttgt
      961 atagtacaac ttaagtttac ctttaatctt aggtgcgata tgtaaataac ctccttctac
     1021 agatgtaatt ttttcacttg taagatctcc gactgtcgtg tttctggaac gtggtgacaa
     1081 atacatatta ttaaagaatg gcgctcttgt catataacct gcattagcaa aaacatagtt
     1141 gcgcccatta actttatagg tagcaccggc tttcacgcca acatcaggaa acacataagt
     1201 ttttgatttg ccataagaat cgtttggaaa caatccattt ttataattac cttctctgta
     1261 aacagaggta t
//
LOCUS       DAORQT010000375         1261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23842_length_1261_cov_9.352405, whole genome shotgun sequence.
ACCESSION   DAORQT010000375 DAORQT010000000
VERSION     DAORQT010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23842_length_1261_cov_9.352405"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK134_09085"
     CDS             <1..253
                     /locus_tag="PK134_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRB39044.1"
                     /translation="FKSKASHIRTYERGVEDETLACGTGITASAIAAHYSGLTKKLSV
                     NMIARGGSLNVTFQVEANKYKNVMLTGSAMNVFDGEIKI"
     gene            complement(243..>1261)
                     /locus_tag="PK134_09090"
     CDS             complement(243..>1261)
                     /locus_tag="PK134_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB39045.1"
                     /translation="PLASENYTYYQNNGFKSNFNHWIKGTLHLGEHFIAYADIQYRYV
                     HYRFTGFDDFATTTNQVAEFHFFNPKAGITFLRNENESAWLSIAVGNKEPSRDDFTES
                     TAKSRPKSENLIDFEAGYSIAIKKLKLSLNAFYMQYKDQLILTGKINDVGNYTRQNVK
                     NSFRRGLEAEFNYDVGKFLAVSGNVALSENKILNFKEYIDQYDADFNYTGQQKIIYKK
                     TDIAFSPSIITAVGIEIRPLKMLSIKLTEKYVGKQYLDNTSSEEKSLKAFAYTNLSIH
                     YTFKIFKTIETEAGVAVYNLFNEMYSSNGYTYGYLYDGMRVNENFYYPQAGTHTMLFA
                     KFRF"
BASE COUNT          390 a          210 c          219 g          442 t
ORIGIN      
        1 atttaaaagc aaagcatcac acatcagaac ttatgaacgc ggggtagagg atgaaactct
       61 tgcctgtggc accggcataa ctgcatctgc aatagcagca cattattccg gtttaacaaa
      121 gaaattatcg gtgaatatga ttgcacgtgg tggaagtctg aatgttactt tccaggttga
      181 agctaacaaa tacaaaaatg ttatgctaac aggttctgct atgaatgttt ttgatggtga
      241 aattaaaatc tgaatttagc aaataacatg gtatgtgttc ctgcttgagg atagtaaaag
      301 ttttcgttaa ccctcatacc atcatataag tacccataag tataaccatt acttgaatac
      361 atttcattga acaggttata tactgcaact cctgcttcag tttcaatggt tttaaaaatc
      421 ttaaacgtat aatgtattga aagattggtg taggcaaatg ctttcaatga tttttcctcc
      481 gaggaggtat tatctaaata ttgttttcca acatacttct cagttagttt gatgcttaac
      541 atttttaacg gccttatttc aataccgaca gcagtaatta ttgaaggaga aaaagcaatg
      601 tctgtttttt tatagataat tttctgctga cctgtatagt taaaatctgc atcgtactga
      661 tcaatatatt ccttaaagtt cagaatttta ttttcgctta atgctacatt gcctgaaaca
      721 gctaagaact tacccacatc ataattaaat tctgcttcta aacctcttct gaaactattt
      781 tttacgtttt gtctggtata gttgccaacg tcattgattt ttcctgttaa aatcagttgg
      841 tctttatatt gcatataaaa ggcattaaga ctcagtttta atttttttat tgcgattgaa
      901 taacctgctt caaaatctat gagattttct gatttaggtc gtgattttgc agttgactca
      961 gtgaaatcat ctcttgatgg ttctttgttt ccaacagcga tagacagcca ggcactttcg
     1021 ttttcattac gtaaaaaagt aatgccggct tttggattga aaaaatgaaa ctctgcaacc
     1081 tgattagtgg tggtggcaaa atcatcaaat cctgtaaacc gataatgtac gtagcgatat
     1141 tgaatgtctg catatgcaat gaaatgctcc cccaagtgta aggttccttt tatccagtga
     1201 ttgaaattgc ttttaaatcc attattttga tagtaagtgt aattttctga agctaatgga
     1261 a
//
LOCUS       DAORQT010000376         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_23930_length_1258_cov_6.408146, whole genome shotgun sequence.
ACCESSION   DAORQT010000376 DAORQT010000000
VERSION     DAORQT010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23930_length_1258_cov_6.408146"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PK134_09095"
     CDS             complement(<1..986)
                     /locus_tag="PK134_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRB39046.1"
                     /translation="MINHRQLFLQHVAQTSQAPLALHIVRADGCFLYDDKGKEYIDLI
                     AGISVCNLGHNNSAINEAVKKQIEQYSHIMVYGELIESPQTLFASLLASHLPPNLNCT
                     YFVNSGTEAIEGAMKLAKRTTGTNENFALTNSYHGRKQGSLSLMSNEYFSSAFRPLIP
                     GVKFIDPTKPDFDVLNENTSCIIMELVKAEEGCKPLTYDFVNNVVRICREKNILLVVD
                     ECQTGLGRTGKSFAFENYDIIPDILVLGKALGGGMPLGAFISSKKTMDKLTHNPVLGH
                     ITTFGGHPVCCAAGMAAFAQLTEQKLYDNVEVKEMLFRKLLKHPKIKSVSGKG"
     gene            complement(979..>1258)
                     /locus_tag="PK134_09100"
     CDS             complement(979..>1258)
                     /locus_tag="PK134_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRB39047.1"
                     /translation="CMRNTRGLRRLGSAADDLAYTACGRLDGFFEYGLNAWDVAGGAL
                     LVEEAGGVVADFSGEKDFIFGQEIVACNKAVFDEFMEVIYTSFRNKND"
BASE COUNT          384 a          282 c          197 g          395 t
ORIGIN      
        1 ccttttcccg aaacagattt aattttagga tgctttaata attttcggaa aagcatttct
       61 tttacttcta cattatcata aagtttttgt tcagtcaatt gagcgaatgc tgccattcct
      121 gctgcacaac aaaccggatg cccgccaaaa gtggttatat gacccagaac agggttgtgc
      181 gttaacttgt ccattgtttt cttagatgag ataaatgcac ctaaaggcat tccgccacct
      241 aatgcttttc ctaaaactaa aatatcggga ataatatcat aattttcaaa ggcaaacgac
      301 ttacctgttc tgcccaatcc ggtctgacat tcatcaacaa ctaacagaat atttttctct
      361 ctgcaaattc gcacaacatt atttacaaaa tcgtatgtta gtggcttaca tccttcttcg
      421 gccttcacca attccatgat gatacacgat gtgttttcgt tcaacacatc aaagtcaggt
      481 tttgtcgggt caataaattt aacacccggt attaaaggcc tgaacgctga tgaaaaatat
      541 tcattactca taagactcaa tgagccttgt tttctgccat gatatgaatt agttaatgca
      601 aaattttcat tcgttcctgt agttcttttg gctaacttca tagccccctc tatggcttcg
      661 gttccggaat ttacgaagta ggtgcaattt aaatttggtg gtaaatgcga tgcaagcaag
      721 cttgcaaaca aggtctgcgg gctttctatc aattcgccat aaaccattat gtgactatat
      781 tgctctattt gttttttgac agcctcattg atagcagaat tattatgacc taagttacat
      841 acagaaatac cggcaattaa atcaatgtat tcttttcctt tatcatcata taaaaaacaa
      901 ccatcggcac gcacaatatg caacgccagt ggcgcctgac ttgtttgcgc aacgtgttgc
      961 aaaaaaagtt gccggtgatt aatcattttt atttctgaat gaagtgtaaa taacttccat
     1021 aaactcatca aaaacagcct tattgcaggc tactatttcc tgtccaaaaa taaaatcttt
     1081 ttctcctgaa aaatctgcaa ccacacctcc tgcttcttca actaataaag cgcctcctgc
     1141 aacatcccat gcattgagac catattcgaa aaatccatca aggcgaccac atgcagtata
     1201 agccaaatcg tcagcagcag aaccaagtct tcttagacct cttgtattcc gcatacaa
//
LOCUS       DAORQT010000377         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_24194_length_1248_cov_7.926236, whole genome shotgun sequence.
ACCESSION   DAORQT010000377 DAORQT010000000
VERSION     DAORQT010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24194_length_1248_cov_7.926236"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK134_09105"
     CDS             complement(<1..1087)
                     /locus_tag="PK134_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRB39048.1"
                     /translation="MQTEHESIEIYGAREHNLKNIDLVLPREKLIVFTGLSGSGKSSL
                     AFDTIYAEGQRRYMESFSAYARQFLGSLERPDVDKINGLSPVISIEQKTTNQNPRSTV
                     GTVTEIYDFMRLLFARAGEAFSYMTGEKMVRLSDDQIVEIILRDFDKKNIVVLAPVVK
                     ARKGHYRELFEQIRVSGFIRVRIDGVITEIKKGMQTDRYKTHDIEIVVDKIEINETAS
                     KRLYDTVKTAMRFGKNTLMIIADDQKRVRFFSRNLMCPTSGISYDEPAPNTFSFNSPY
                     GACPRCNGLGTIPEADLASIIPDNKKSIRKGGILPLGEYKNNWIFGELEYIGRRFGFN
                     LNTPIHDIPHEALNIILYGTDDVSLNAT"
BASE COUNT          358 a          248 c          241 g          401 t
ORIGIN      
        1 gagttgcatt gagtgaaaca tcatctgtgc cgtacaatat tatgttcagt gcttcatgcg
       61 gaatatcatg aattggtgtg ttcaggttga agccaaacct tctgccaatg tattccaact
      121 caccaaatat ccagttgttt ttatattcac ctaaaggcaa aattccacct ttgcggattg
      181 attttttatt gtcgggaatg atggaagcta aatctgcttc tggtattgtg ccaagtccgt
      241 tgcaacgtgg acaggcaccg taaggagagt taaaagaaaa tgtgtttggt gcaggttcat
      301 cataagagat tcctgaagta ggacacatca gattacgact gaaaaagcga actctttttt
      361 gatcatcagc aataatcatc aatgtattct tgccaaaacg catggcagtt tttacggtgt
      421 cataaagtct ttttgatgcc gtttcgttaa tttcaatttt gtccactaca atttcaatgt
      481 catgagtttt gtagcggtcg gtttgcatac ctttttttat ctcagtaatc acaccatcaa
      541 ttcgcacacg gataaaacct gatactctta tttgttcaaa cagttcgcgg taatgtcctt
      601 tacgtgcttt gacaacagga gcaagcacaa caatattttt cttgtcaaaa tcacgaagaa
      661 ttatttctac aatctgatca tcactcagtc tcaccatttt ttcacccgtc atgtaggaga
      721 aagcttcacc ggcacgtgca aaaagcaaac gcatgaaatc ataaatttca gtaaccgttc
      781 ctactgttga gcgcggattc tgattggtag ttttttgttc aatgctgata acaggagaga
      841 ggccgtttat tttatctaca tcagggcgtt ccaggctgcc aagaaattgt cttgcatagg
      901 cactgaaact ttccatatag cgtctttgtc cttcagcgta aatagtgtcg aatgccaatg
      961 acgacttacc actgccgctg agtcctgtaa agacaatcag tttttcgcgc ggtaaaacga
     1021 ggtctatatt tttgagatta tgttcccttg ctccgtaaat ttcaatgctc tcgtgctctg
     1081 tttgcatata gtaataacct gcaaaattaa cttattgcag cctcttattt ggatttgtta
     1141 ataaaaatga aggcgctgtt tttcttgatg ctaaatcgtc agcataaatt cattgcaaga
     1201 aaatctggca gcagcaatta tcaaagcaac agcgtgggat aaaaaatg
//
LOCUS       DAORQT010000378         1243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_24347_length_1243_cov_4.638889, whole genome shotgun sequence.
ACCESSION   DAORQT010000378 DAORQT010000000
VERSION     DAORQT010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24347_length_1243_cov_4.638889"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..816)
                     /locus_tag="PK134_09110"
     CDS             complement(82..816)
                     /locus_tag="PK134_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HRB39049.1"
                     /translation="MFSFFKRKDSTPANFLEVKTDMHSHLIPGIDDGAKTIEDSLTLI
                     KELHALGYTKLITTPHIMSDFYRNTPETILAGLEEVRKAVKAENIPVTIEAAAEYYLD
                     DGFIHKLEEEKLLTIGNNNHLLFEISYVNAPDNLLQVIFRMQVLGYKPIMAHPERYPF
                     WGNNFDFYGSLREQGVLLQINVNSLGGYYGPDAKRIAEKLIEKEWVDLIGTDTHATKH
                     IQALHKTVREKSFRKLLEFNLLNKHL"
     gene            complement(920..>1243)
                     /locus_tag="PK134_09115"
     CDS             complement(920..>1243)
                     /locus_tag="PK134_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB39050.1"
                     /translation="IVNNAFYALNERNKNSPANENYHPELQIITKNVNKKIYLHIKDN
                     AMGIPAELQAKIFEPFVTTKPAGEGTGLGLSICRDIIRNLKGDIMLISENGKGTEFII
                     TLPVE"
BASE COUNT          361 a          232 c          227 g          423 t
ORIGIN      
        1 taccacaaca ccaaatttca tgattgattt ttttactgac tacaaaagta acgataaatt
       61 aaactgcttt aacaatcttt ttcagagatg tttgttgagc aaattaaact caagaagttt
      121 cctgaatgat ttttcacgta cggttttatg caatgcctga atgtgtttgg tggcatgtgt
      181 gtcggtgcca ataaggtcaa cccattcttt ttctataagt ttttctgcaa tacgtttggc
      241 atcgggacca tagtacccgc caagagagtt tacatttatc tgtaacaaaa caccttgttc
      301 gcgcagactg ccatagaagt cgaaattatt tccccagaaa ggataacgtt ccggatgtgc
      361 cataatgggt ttgtagccca gcacctgcat tctgaaaatt acctgtaata aattatcggg
      421 agcgtttaca taactgattt caaaaagcaa atggttgttg tttccaatgg tcaacaactt
      481 ttcttcttct aacttatgta taaatccatc gtcaagatag tattcggcag cggcctcaat
      541 cgttacagga atgttttctg cctttactgc tttgcgcact tcttcaagcc cagccaaaat
      601 tgtttcgggc gtattgcgat aaaaatcgct catgatatgc ggtgtagtaa tgagctttgt
      661 gtagcctaat gcatgcagtt cttttatcag cgtaagagag tcttcaatgg tttttgcacc
      721 atcatcaatc cccggaatga ggtgactgtg catgtcggtt ttaacttcta aaaaattggc
      781 cggtgttgaa tcttttcttt taaaaaagga gaacataatt tacttcctgt aattaaggca
      841 acaaaagtat aagaaaatat tgcaatatac attgctatct gtaaggtaat taaacatctt
      901 catgtagcag caatgctttt tattcaacag gcaaggtaat aataaattct gtcccttttc
      961 cattttcaga aatcagcatg atgtcacctt tcagattccg gataatatct ctgcagatac
     1021 ttaaaccgag tcctgttcct tcacctgcag gcttggtggt aacaaaaggt tcaaaaattt
     1081 tggcttgcaa ctctgcagga atgcccatag cattgtcttt aatgtgcaga taaatttttt
     1141 tgtttacatt ctttgtgatt atctgtaatt cgggatgata attttcgtta gcaggagaat
     1201 ttttattcct ttcgttgagt gcataaaaag cattatttac aat
//
LOCUS       DAORQT010000379         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_24455_length_1239_cov_5.479730, whole genome shotgun sequence.
ACCESSION   DAORQT010000379 DAORQT010000000
VERSION     DAORQT010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24455_length_1239_cov_5.479730"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1239
                     /locus_tag="PK134_09120"
     CDS             <1..>1239
                     /locus_tag="PK134_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRB39051.1"
                     /translation="CMAIQQKKSNYDTDIFQPTINYIEAMSGIKYGSSEQTDIAMRVL
                     SDHIRAIAFTIADGQLPSNNKAGYVIRRILRRAVRYSFTYLNFKTPFIHRLTPLLADQ
                     FKEVFPELFEQKDFVQKVIKEEEQSFLRTLETGIRRFDLYNKSVIDGEFAFELYDTYG
                     FPIDLTQLIARERNMQVDMNGFNQCLEQQKERSRAASAVDAEDWVILENSESKFIGYD
                     NTTAEVNIIKYRKVKSKNKEQYQLVFNQTPFYAESGGQVGDTGYIQNEKEKIFITDTQ
                     KENNQIIHLSDKLPVDLKADFIATVNNSRRRKITANHSATHLMHAALKQILGKHVVQK
                     GSLVNDEHTRFDFAHFAKVSDEDIAKIEQLVNEKIRENIALDERRNVPIEEAKSMGAT
                     ALFGEKYGDYVRVIIFDPAY"
BASE COUNT          457 a          196 c          231 g          355 t
ORIGIN      
        1 gtgtatggca atacaacaaa aaaaatcaaa ttatgacaca gatatttttc aacctaccat
       61 aaattacatt gaagcaatga gtggtataaa atatggcagt agcgagcaaa cagatattgc
      121 catgcgcgtt ttgtctgatc atatcagagc tattgcattt accattgcag atggacaact
      181 tccgtcaaac aataaagcag gttatgttat tcgaagaata cttagaagag ccgttcggta
      241 ttcttttact tatctgaatt ttaaaactcc atttattcat agactgacac ctttattggc
      301 agatcagttt aaagaagttt ttcctgagct ttttgaacag aaagattttg tacaaaaagt
      361 tatcaaggaa gaagagcaat cttttttacg cactcttgaa acaggtatca gaagatttga
      421 tctatataac aagtctgtta ttgatggaga gtttgcattc gaattatacg acacttatgg
      481 ttttccaatt gaccttactc aattaattgc acgtgagcga aacatgcaag ttgacatgaa
      541 tggatttaac caatgtttag aacagcaaaa agaaagatcc agagctgcat cagcagttga
      601 tgctgaagac tgggttattc ttgaaaacag tgaatcgaaa ttcatcggat atgacaatac
      661 aactgctgag gtaaatatta taaaatacag aaaggtaaaa tcaaaaaata aagaacaata
      721 tcagcttgtc ttcaatcaga ctccttttta tgcagagagc ggtgggcaag ttggtgatac
      781 aggctatatc caaaacgaaa aagagaaaat ttttattacc gacacacaaa aagaaaataa
      841 tcagatcatt cacctgtcag ataaattgcc tgttgatttg aaagcagatt ttattgcaac
      901 agtcaacaat tcgagaagaa gaaaaataac agccaatcac agtgcaacac atttaatgca
      961 tgcagcatta aagcagattt taggtaagca cgtagtacaa aaaggttcac ttgtgaatga
     1021 tgagcacaca cgttttgatt ttgctcattt tgcaaaagtt tctgatgaag atatagccaa
     1081 aatagaacaa ttggtaaatg aaaaaattcg tgagaatata gctttagacg aacgcagaaa
     1141 tgtgcctatt gaagaagcta aatcaatggg agctactgca ttatttggcg agaaatatgg
     1201 cgactatgtt cgagttatca tatttgatcc tgcatacag
//
LOCUS       DAORQT010000380         1223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_24899_length_1223_cov_3.678082, whole genome shotgun sequence.
ACCESSION   DAORQT010000380 DAORQT010000000
VERSION     DAORQT010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24899_length_1223_cov_3.678082"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PK134_09125"
     CDS             <1..644
                     /locus_tag="PK134_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39052.1"
                     /translation="ITEDGTTLYFASDMPGGAGGKDIWMTKNENGAWGTPVNISSINT
                     VGDEMFPYIAPNGKLYFSSNGHMGMGGLDIFSATSENGAWANVTNLKYPINSSWDDFG
                     FMFNSATTGYLTSNRDGGQGQDDIYSFVVPPANFAVYGRVYDTDTKEAIAGATVELFG
                     SDGTQLSVKTQDAGTYRYELKPDVRINYCNHVFLYAATTDEKNESRKAIQRKY"
     gene            601..813
                     /gene="yajC"
                     /locus_tag="PK134_09130"
     CDS             601..813
                     /gene="yajC"
                     /locus_tag="PK134_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRB39053.1"
                     /translation="MKKMKVEKQFKESINKGDKVVTIGGVHGKITEVGETFFMIEIDN
                     NVKIKIDKTAVSAEASKQYRKTDDKK"
     gene            826..>1223
                     /locus_tag="PK134_09135"
     CDS             826..>1223
                     /locus_tag="PK134_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39054.1"
                     /translation="MNIFKNIRLKGNASLPVFFICVFVSLVFWILYSLNKNYVYPVTY
                     TVTLQNTPFSKQVISEIPQQVNVTLRSRGFELFWYLIRNRERNLTVDLKKYSVNNEKL
                     GISLQSEFRNQNQNPTNGVLTSTDEILEKG"
BASE COUNT          425 a          195 c          236 g          367 t
ORIGIN      
        1 caattactga agatggaacc acactttatt ttgcatcaga tatgcctggt ggtgcaggtg
       61 gaaaagacat ctggatgact aaaaatgaaa atggagcatg gggtacgcct gtaaacatct
      121 cttctattaa tactgtaggt gatgaaatgt tcccatatat tgcaccaaac ggaaaattgt
      181 atttctcatc aaatggtcac atgggcatgg gtggacttga tattttctct gcaacttcag
      241 aaaatggagc atgggcaaat gttactaact taaagtatcc aattaactca tcatgggatg
      301 atttcggatt tatgttcaat tctgccacta caggttattt gacttcaaac cgcgatggcg
      361 gacaaggaca ggatgatatt tattcgttcg tagtacctcc tgcaaatttt gcagtttatg
      421 gtcgtgtata tgatacagac actaaagaag caattgcagg tgcaacagtt gaactttttg
      481 gtagcgatgg aacacagctt tctgttaaaa cacaagatgc gggaacatat cgctatgagt
      541 taaaacctga tgttaggatt aattattgta atcatgtatt tctttatgct gcgaccacag
      601 atgaaaaaaa tgaaagtcga aaagcaattc aaagaaagta ttaataaagg tgataaagta
      661 gttaccattg gtggcgtgca tggaaaaatt actgaagttg gtgaaacttt ttttatgatt
      721 gagattgaca acaacgtaaa aatcaaaatt gataaaactg ccgtttctgc agaagcatca
      781 aagcagtacc gcaaaacaga cgataaaaaa taaaaagcct tacacttgaa tattttcaaa
      841 aatatcaggt taaaaggcaa tgcatcattg cccgtatttt ttatttgtgt ttttgtgtcg
      901 cttgttttct ggattttata ctcattgaac aagaattatg tatatcctgt aacttatact
      961 gttactcttc agaatacacc attcagcaag caagtcatca gcgaaatacc tcaacaggta
     1021 aatgtaacac ttcgttcaag agggtttgag ttgttttggt atttaatcag aaaccgcgaa
     1081 agaaacttaa cagttgatct gaaaaaatat tctgtaaaca atgaaaaact tggtatcagt
     1141 ttacaaagtg aattcagaaa tcagaatcaa aaccctacta acggtgtgtt aacttcaaca
     1201 gatgaaattc ttgaaaaagg aat
//
LOCUS       DAORQT010000381         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_24951_length_1221_cov_5.147513, whole genome shotgun sequence.
ACCESSION   DAORQT010000381 DAORQT010000000
VERSION     DAORQT010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24951_length_1221_cov_5.147513"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK134_09140"
     CDS             <1..435
                     /locus_tag="PK134_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HRB39055.1"
                     /translation="YFFYKASGVYVLLVLAAAVIDFNLSNFIYNATSDFRRKALLVFS
                     VVINLGLLAYFKYTNLLIDLYNAILTGNVQPIDVYLPIGISFYTFENLSYTIDVYRRQ
                     FVPVKKFIDYLFFLSFFPKLMMGPIVRAADFILHMMYLKACF"
     gene            447..1139
                     /locus_tag="PK134_09145"
     CDS             447..1139
                     /locus_tag="PK134_09145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRB39056.1"
                     /translation="MFKKVVISDFIYLNLVQYVFDDPLRHTGMECLFAVYGYALVIYC
                     DFSGYSDMAIGIAKWIGIDININFLAPYQSASITEFWRRWHISLSSWLRDYLYIPLGG
                     NRKGEVRTYINLFITMLLGGLWHGASLNFIFWGALHGVALAFHKLWKAAYPSHFSGRL
                     TRIAGIIITFHFVCLCWIFFKCTTFTDSFNMIHQIVFNFYAEGWTLFSANYSEVLLVM
                     LLGFVLHFLPIR"
BASE COUNT          325 a          199 c          238 g          459 t
ORIGIN      
        1 tatttctttt ataaagcctc aggagtttat gtgcttttgg ttcttgcggc tgccgtaatt
       61 gattttaacc tcagtaattt tatttacaat gcaacatctg attttcggag aaaagcatta
      121 ctcgttttca gcgttgtcat caatcttgga ctacttgctt atttcaaata caccaacctg
      181 ttaattgact tgtataatgc catactaacc ggaaatgttc agccaataga tgtctatctg
      241 ccaataggta tatcatttta cactttcgaa aatctgagtt atactattga tgtttacaga
      301 agacagtttg tgccggtaaa aaaatttatt gactacctgt tttttctttc atttttccca
      361 aaactaatga tgggaccgat agtgcgtgca gcagatttca tactgcacat gatgtatcta
      421 aaggcttgtt tttaattctg accggattgt ttaagaaggt tgttatttca gactttatct
      481 atctcaattt agttcagtat gtgtttgatg atcctttgcg gcacacaggt atggaatgtc
      541 tttttgcagt gtatggctat gcattggtaa tttattgtga tttttccggc tatagtgata
      601 tggcaatagg tattgcaaag tggataggta tagatatcaa tattaacttt cttgcaccct
      661 atcagtcggc ctccataaca gagttctggc gcagatggca catctcactt tcatcgtggt
      721 tgcgcgatta tctttatatt ccgttaggtg gtaatcgtaa aggagaagta agaacctaca
      781 ttaatttatt tatcaccatg ttgcttggtg gactatggca tggtgccagc ttgaatttta
      841 ttttttgggg tgcattacat ggtgtggcat tagcttttca taagttatgg aaggcagcat
      901 atccatcaca tttttccgga cgtttaacac gcattgcagg catcattata acatttcatt
      961 ttgtttgtct ttgctggata ttttttaagt gcacaacctt tactgacagt tttaacatga
     1021 ttcatcagat tgtgtttaac ttctatgctg aaggttggac attattcagt gccaattata
     1081 gtgaagtgtt gttagtgatg ttactaggat ttgtattgca ctttttgcca atccgataga
     1141 atgaaattat tatcaattat actgaaaagt ggaattggtt actaaaggca tctgtagcaa
     1201 cggcagttgt aatttttgta a
//
LOCUS       DAORQT010000382         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_25278_length_1210_cov_3.610390, whole genome shotgun sequence.
ACCESSION   DAORQT010000382 DAORQT010000000
VERSION     DAORQT010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25278_length_1210_cov_3.610390"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PK134_09150"
     CDS             <1..845
                     /locus_tag="PK134_09150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB39057.1"
                     /translation="SHAHTAAFLSAWLFGNKCPLVISRRVDFPVSSSWFSHLKYNHSS
                     IKKIICVSDAIRQMTAPAIKRKNILEVVHSGVDTEKFVYGKTKLHHEFNLSEETFIVG
                     NVSALADHKDLFTFIDTAEIVIKQTQHVHFFLIGDGAELNALQKKVADKNLTSHIHFT
                     GFRKDVNELMPELNLFLMTSETEGLGTTILDAFAAKVPVVATNAGGIPEIVHHGKTGL
                     IAPVGDAKQLAAHVLTLMNDNELKERFIANSHQLLKEFTFRRTAEKTKAIYSVVLYGE
                     KENV"
     gene            845..1210
                     /locus_tag="PK134_09155"
                     /pseudo
     CDS             845..1210
                     /locus_tag="PK134_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863028.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
BASE COUNT          404 a          212 c          230 g          364 t
ORIGIN      
        1 actcacatgc acataccgct gcattcttat cagcttggct gtttggcaat aaatgccctt
       61 tggtgataag ccgcagagtt gactttccgg tttcttcttc ctggttttct catttgaagt
      121 acaaccactc ttcaataaaa aaaattatct gcgtgtctga tgctatcaga caaatgactg
      181 cacctgccat taaaagaaaa aatattttag aagttgttca ttcaggagtt gacaccgaaa
      241 agtttgttta cggaaaaacg aagctacatc atgaatttaa tctttcagaa gaaacgttta
      301 tcgtaggaaa tgtttctgca ttggccgacc ataaagattt gtttacgttt attgatacag
      361 cagaaattgt tataaaacaa actcaacacg ttcatttttt tctgattggt gatggcgcag
      421 aattgaatgc acttcagaaa aaagtggctg acaaaaatct tacttctcat attcatttta
      481 ccggattcag aaaagatgta aatgaattga tgcctgagct caatcttttt ctgatgacat
      541 cggaaacgga aggcttgggc accacaattt tggatgcttt tgcagctaaa gttcctgtag
      601 tggcaaccaa tgcaggtggc attcctgaaa ttgtacatca tggcaaaacc ggtttaatag
      661 caccggttgg tgatgcaaaa caacttgcag cacatgttct gactttaatg aatgacaacg
      721 aattgaaaga aagatttata gccaattcac atcaattgct aaaggaattt actttcaggc
      781 gaaccgcaga aaaaacaaaa gctatctatt cagttgtttt atacggtgag aaagaaaatg
      841 tgtaatggct gttaaaaacg aacatatcaa aattattctt gatggacttc caaatgaacc
      901 gggggtttat cagttttttg ataagtctga aaaaataatt tatgtaggca aagccaagag
      961 tttacagtta tttttcgaaa gatgtttttg agagtggaaa aacggccatc atggtgcggc
     1021 aggttgtgaa tattcgtttt atcattacag aaactgaact tgatgcactg ttgcttgaaa
     1081 acaacctgat aaaaaaacat cagcctcgat acaacatcct cctcaaagac gataaaacat
     1141 atccatggat ttgtataaaa aatgagcctt tcccaagagt attttataca cgcagaaaaa
     1201 ttcgtgatgg
//
LOCUS       DAORQT010000383         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_25865_length_1192_cov_5.176781, whole genome shotgun sequence.
ACCESSION   DAORQT010000383 DAORQT010000000
VERSION     DAORQT010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25865_length_1192_cov_5.176781"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PK134_09160"
     CDS             <1..524
                     /locus_tag="PK134_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRB39058.1"
                     /translation="YNVTKIVTACPHCFNTLKNEYPQLGGHYEVVHHSMLLQQLINDG
                     KLIINGGKFLGKKIVFHDPCYLGRGNDVYEAPRDVIKKLDAELFEMKRSRANGFCCGA
                     GGAQMFKEPENGTKDVNIERTEEILEAKPDYVAVGCPFCMTMLTDGVKNFNKEDSVKV
                     LDIAELIEKANDL"
     gene            complement(575..>1192)
                     /locus_tag="PK134_09165"
     CDS             complement(575..>1192)
                     /locus_tag="PK134_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRB39059.1"
                     /translation="ADILSSGQVSQALALTGIVAKNQELSSTQQLAHLGKSLLSVAKR
                     EQKLYALEYLKYYPFMGKDKHVPFWLDKVDGNRTDNFLYHLLFNTSLPSLLQYEDRNS
                     MAFSIESRVPFLDHRLVEFSFGLRNEEKANGIYTKWIMRSALQNVLPPKIAWRKDKKG
                     FVTPGEVKWLRGPLAHLIESDFNKLDFLNQHKVKPIKTVITVRHY"
BASE COUNT          382 a          209 c          231 g          370 t
ORIGIN      
        1 gctataatgt tacaaaaatt gtaactgctt gtccgcattg ttttaataca cttaaaaatg
       61 aatatccaca gttaggtggc cactatgaag ttgtgcatca cagcatgttg ttgcaacaat
      121 tgataaatga tggaaaactg attatcaatg gcggaaaatt tttaggtaag aaaattgtat
      181 ttcacgaccc atgttattta ggtcgtggca acgatgtata tgaagctcca cgtgatgtga
      241 ttaaaaaatt agatgcagag ttatttgaga tgaaacgaag ccgtgccaac ggattttgtt
      301 gtggtgcagg tggtgcacaa atgtttaaag aacctgaaaa tggaactaaa gatgtgaata
      361 tagaacgtac agaagaaatt cttgaagcta aaccagacta tgtagctgtt ggctgcccct
      421 tttgtatgac tatgcttact gacggtgtga aaaatttcaa caaagaagat tctgtaaaag
      481 ttcttgacat tgctgaattg attgaaaaag caaacgattt gtaaaaatca acagaagtgt
      541 ttttgccagt aggctaaagt gcaaagtcgc cacactaata atgcctgact gttatcaccg
      601 tttttatagg tttaacttta tgttgattca gaaaatcaag tttattaaaa tcgctttcta
      661 ttaaatgtgc taaagggcct cttagccatt tcacctcacc gggtgtcaca aaacctttct
      721 tatccttacg ccaggcaatt ttaggtggta atacattctg taaggcacta cgcattatcc
      781 atttggtgta tatgccattg gctttttctt cattccttaa tccaaaagaa aattccacca
      841 agcgatgatc taaaaacggc acacgcgatt caatggagaa agccatgctg ttacggtctt
      901 cgtattgcag cagtgatggc aaacttgtat tgaaaagaag atggtataaa aaattatctg
      961 tcctgttgcc atcaaccttg tctaaccaaa atggaacatg tttgtcttta cccataaacg
     1021 gataatattt caggtattca agtgcatata atttttgttc acgtttagca acacttaata
     1081 aacttttacc taaatgagct aattgctgcg tagaagacag ttcctgattc ttagccacaa
     1141 tgcctgtaag tgctaatgcc tgactaactt gtcctgacga gagtatatcg gc
//
LOCUS       DAORQT010000384         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_25876_length_1192_cov_3.703606, whole genome shotgun sequence.
ACCESSION   DAORQT010000384 DAORQT010000000
VERSION     DAORQT010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25876_length_1192_cov_3.703606"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK134_09170"
     CDS             <1..407
                     /locus_tag="PK134_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB39060.1"
                     /translation="KYPGVPEVNMITIDEHPFNIEGLEFIPIEVLHYKLPVNAYRVGD
                     FTYITDANFISEKEKEKIKGSRIVVINALRREKHVSHFNLEEALELIKELKPEKAYLT
                     HISHQLGKHSDVEKELPPNVFLAYDGFEIEMS"
     gene            468..>1192
                     /locus_tag="PK134_09175"
     CDS             468..>1192
                     /locus_tag="PK134_09175"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRB39061.1"
                     /translation="MQVTIDSNSGFCFGVVYAIQQAEEELEVNGHLYCLGDIVHNNME
                     VDRLKGKGLEIINHEQLQQLHDCKVLIRAHGEPPSTYQTAIKNNIELIDASCPVVLKL
                     QNRVRGSYDEITDTGNAQVVIYGEKGHAEVNGLVGQTGGKAIVVKTEEDLDQIDFNKP
                     VHFFSQTTKSTLGFEQMQKTIQQRIVKQKGSLEETDFITNDTLCRQVSNREPQMRRFS
                     SQHDVVVFVSGKKSSNGKALYSV"
BASE COUNT          411 a          201 c          243 g          337 t
ORIGIN      
        1 ttaaatatcc gggtgtgcct gaagtgaata tgattaccat tgatgagcac ccttttaata
       61 ttgaaggact ggagtttatt cccattgaag tgctgcatta caaattacct gtcaatgctt
      121 atagggttgg tgattttacc tacataacag atgctaactt tatttcagaa aaagaaaaag
      181 aaaaaataaa aggcagtcgt attgtagtta tcaatgcgct aagacgcgaa aaacatgttt
      241 ctcattttaa tcttgaagaa gcacttgagt tgattaaaga attgaagccc gaaaaagcct
      301 acctcacaca catcagccat cagcttggaa aacacagtga tgtagaaaag gaattaccgc
      361 caaatgtgtt tttagcttac gatggttttg aaatagaaat gtcataagat taaaccatag
      421 ggcgtttaaa acgttattgt cttacttttg cacataattt gttcactatg caggttacta
      481 ttgattccaa ttcaggcttt tgctttggtg tagtttatgc catacagcag gctgaagaag
      541 agcttgaagt taacggtcac ttatattgtc ttggcgacat tgtacacaac aacatggaag
      601 ttgacagact taaaggtaaa ggtttggaaa ttatcaatca tgaacagttg caacagttgc
      661 acgattgcaa agtactaatc agagcacatg gcgaaccacc atcaacatat caaacggcaa
      721 ttaaaaacaa tattgagctt attgatgcat cttgtcctgt tgtgctgaag ttacaaaatc
      781 gtgtcagagg cagttatgat gaaattacag atacgggaaa tgctcaggtg gtgatttatg
      841 gcgagaaagg tcatgcagaa gtgaatggtt tggtgggcca gacaggtgga aaagccattg
      901 tagttaaaac agaagaagat cttgatcaga tagatttcaa taaaccggtt catttttttt
      961 ctcagacaac aaaaagcaca cttggttttg aacaaatgca aaaaaccatt cagcaacgaa
     1021 tagtaaaaca aaaaggctcg cttgaagaaa ccgattttat taccaacgac actttatgcc
     1081 gtcaggttag caaccgcgaa ccacagatgc gcagattttc atcacaacat gatgttgtag
     1141 tttttgtaag cggaaaaaaa tcatcaaacg gaaaagcttt atacagtgtc tg
//
LOCUS       DAORQT010000385         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_26186_length_1182_cov_7.960958, whole genome shotgun sequence.
ACCESSION   DAORQT010000385 DAORQT010000000
VERSION     DAORQT010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26186_length_1182_cov_7.960958"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK134_09180"
     CDS             complement(<1..487)
                     /locus_tag="PK134_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39062.1"
                     /translation="MKTFSKLINTVFIFAVTVFVAQAQSPTNQDCPNAIPICNTTYST
                     VISYSGTGNIPNEINNGSSCLLSGERNDVWYTFTTPFFGNLNFTITPNNPADDYDWAV
                     YNLTTATCADIFTNPSIEVSCNYAPNLGCNGTTGPNNQTAGPCGGQNNPIIPVTPGQT
                     YV"
     gene            623..>1182
                     /locus_tag="PK134_09185"
     CDS             623..>1182
                     /locus_tag="PK134_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRB39063.1"
                     /translation="MKVTVVGAGNVGATCANVIAHRELVNEVVLLDIKEGTAEGKSLD
                     MWQTAPINLYDTRIKGSTNDYAATAGSDVVVITSGLPRKPGMSRDDLIATNAGIVRSV
                     TENIVKHSPNTIIIVVSNPLDVMTYCAFLTSGFDSKRVFGMAGILDTARYRAFLAEAL
                     NCSPKDIHAVLMGGHGDTMVPLPRYTT"
BASE COUNT          368 a          201 c          237 g          376 t
ORIGIN      
        1 ttacataagt ctgacctggt gtaacaggaa ttattggatt gttttgtcca ccacatggcc
       61 ctgctgtttg attattgggt cctgtagttc cattgcaacc aagattaggt gcataattgc
      121 aactgacttc tattgatggg ttggtgaaaa tatcggcaca agttgcagta gtaaggttat
      181 aaactgccca gtcataatcg tcagcaggat tgtttggtgt aatggtaaaa ttaagatttc
      241 cgaaaaatgg tgttgtaaat gtgtaccaaa catcattgcg ctcaccactt aaaagacagg
      301 aacttccgtt attaatttca ttcggaatat ttcctgttcc actgtatgaa atgactgtgg
      361 agtaagtagt gttacagatt ggaattgcat tcggacaatc ctgatttgta ggagattgtg
      421 cctgtgcaac aaaaacggtt acagcaaaaa tgaatactgt attaattaat ttggaaaagg
      481 ttttcacacc acaaatatac aatttcactt aataatcaaa cacattcacc aaaaaataca
      541 ccgttaaatt ttttaacatt tagcgtcatg caacattttt ttaaataatt tcgccctctg
      601 aaaaatttta atttagtata aaatgaaagt aacagttgta ggtgcaggaa atgtaggagc
      661 tacctgtgca aatgtgattg ctcaccgcga attggttaac gaagttgtat tgctcgatat
      721 taaagaaggt actgctgaag gaaaatcatt ggatatgtgg caaaccgctc caatcaatct
      781 ttatgataca agaataaaag gttctacaaa cgattatgct gcaacagcag gctctgatgt
      841 tgttgtaata acatcaggtc ttccacgcaa gccgggcatg agtcgtgacg atttaatcgc
      901 taccaatgca ggtatcgttc gttctgtgac tgaaaatatt gttaaacatt ctccaaatac
      961 catcatcatt gtagtttcca atcctttgga tgtaatgacc tattgtgcat tcttgacatc
     1021 aggatttgac tctaagagag ttttcggtat ggcaggaatt ttagatactg cacgttacag
     1081 agcattttta gctgaggcat taaattgttc tccaaaagat attcatgctg tattgatggg
     1141 tggtcatggt gatactatgg tgcctttgcc aagatataca ac
//
LOCUS       DAORQT010000386         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_26753_length_1166_cov_3.896490, whole genome shotgun sequence.
ACCESSION   DAORQT010000386 DAORQT010000000
VERSION     DAORQT010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26753_length_1166_cov_3.896490"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1166
                     /locus_tag="PK134_09190"
     CDS             <1..>1166
                     /locus_tag="PK134_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRB39064.1"
                     /translation="IGLHQRDNERLIKALKDLRDIGNTVIVVEHDRETIEAADYVVDL
                     GPGAGINGGDIVAIGKPEELKYQQSLTANYMSGGIKMPSSKNRKGNGKKLTIAGAKGN
                     NLKNVTALFPLGTLICVTGVSGSGKSTLVNETIYPALNKHFYNSTQKPLPYEKINGLS
                     HIDKVIEIDQSPIGRTPRSNPATYTGVFTDIRNLFSQLPEAKIRGYAPGVFSFNVSGG
                     RCDTCLGAGLKIIQMNFLPDVHVHCETCNGKRYKREILEVRYRGKSISDVLNLSINEA
                     VDFFSNIPAILHKIKTLQDVGLGYITLGQSSTTLSGGEAQRVKLASELSKKDTGNTFY
                     ILDEPTTGLHFEDVRILLLVLQQLVDKGNTVVVIEHNMDIIKAADYIIDLGPEG"
BASE COUNT          377 a          205 c          246 g          338 t
ORIGIN      
        1 ctataggact gcatcagcgc gataatgaac gactgataaa agcattgaaa gaccttcgcg
       61 atatcggcaa taccgtcatc gttgttgagc atgacagaga aactattgaa gctgccgatt
      121 atgttgttga ccttggcccc ggtgcaggta ttaacggtgg tgacattgta gcaataggta
      181 agcctgaaga gctaaagtat cagcaatcac tcacagcaaa ttatatgagt ggtggcatta
      241 agatgccttc ttcaaaaaac agaaagggca atggaaaaaa attaaccatt gcaggtgcta
      301 aaggaaacaa tctaaaaaat gtgactgcac tttttccttt aggaacatta atttgcgtta
      361 caggtgtttc aggaagcggc aagtcaacct tggttaatga aactatttat cctgcattga
      421 acaaacattt ttataacagc acgcaaaaac cattgcctta tgaaaaaata aatggattga
      481 gtcatattga taaagttatt gagattgacc aatcgccaat tggccgtaca ccacgtagta
      541 atcctgctac atacacagga gtttttactg acataagaaa tttgttttca caattgcccg
      601 aagcaaaaat aagaggttat gccccgggag ttttttcatt caacgttagt ggtggccggt
      661 gtgatacttg tctgggtgct ggcttgaaaa ttatacagat gaactttttg cctgatgtgc
      721 acgtacattg tgagacttgc aacggcaaac gctacaaacg cgaaatactt gaagttcgtt
      781 atcgtggtaa atctatcagc gatgtattga atctgagtat caatgaagct gttgattttt
      841 tcagcaacat tcccgctatc ttgcataaaa ttaaaacgtt gcaagacgtt ggtttaggtt
      901 acataacatt agggcaatca tcaactaccc tatcaggtgg tgaagcacaa cgggtgaagt
      961 tagcatcgga actttcaaaa aaagatacag gcaatacttt ttatattctt gatgagccaa
     1021 caacaggcct gcattttgaa gatgtaagaa ttttactttt ggttttacag caattggttg
     1081 acaaaggcaa caccgttgta gttattgaac ataacatgga tattattaaa gctgctgatt
     1141 atattattga tcttggtcct gaagga
//
LOCUS       DAORQT010000387         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_27040_length_1157_cov_3.014519, whole genome shotgun sequence.
ACCESSION   DAORQT010000387 DAORQT010000000
VERSION     DAORQT010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27040_length_1157_cov_3.014519"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="PK134_09195"
     CDS             complement(<1..119)
                     /locus_tag="PK134_09195"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB39065.1"
                     /translation="MILQTDTICALITPPGRGTVGVIRISGMKTFEIISKIFTP"
     gene            complement(116..>362)
                     /locus_tag="PK134_09200"
     CDS             complement(116..>362)
                     /locus_tag="PK134_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRB39066.1"
                     /translation="VYTTPLSSGCVNGVMRKVIIQLTSAIEQDISIEQLIDVDEIFLT
                     NTGSGIRPVNKMQGKVFGTNFGMQLTNRLNNFIIEQK"
     assembly_gap    363..376
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<377..950)
                     /locus_tag="PK134_09205"
     CDS             complement(<377..950)
                     /locus_tag="PK134_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRB39067.1"
                     /translation="MKWAYYDGQWVDAATPVLKLNNRGFIYGDGLFESIRCSRGKANF
                     LSLHIERLLAGVAVLNLKCDFITEDYLREIILEMNQRNNFTDGSKLRLLITRNAGGTY
                     LPEQNNASLCMLPITAVESEYTIPKCFIELGIYSEQQKPINKLSGFKNCNSLLYVLAS
                     IYAQEMKFDDCLILNSKNMLCEATASNVFWL"
     gene            complement(931..>1157)
                     /locus_tag="PK134_09210"
     CDS             complement(931..>1157)
                     /locus_tag="PK134_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRB39068.1"
                     /translation="PEKSKCLCRKPDSLLLEKAIARYKVDKNNAHFIGDMETDMIAAN
                     RAGVKGHLIESNSNIVELARKICQHEVGVL"
BASE COUNT          351 a          228 c          178 g          386 t
ORIGIN      
        1 ggagtaaata tttttgaaat aatttcaaat gttttcattc cagatattct gatcacacca
       61 acagtaccac gacctggtgg tgtaattaaa gcacaaatgg tgtcagtctg taatatcatt
      121 tttgttctat aataaaatta ttaagccgat tagtaagttg catgccaaaa ttagttccaa
      181 atactttacc ctgcatttta ttcaccggtc tgatacctga gcctgtattg gtaagaaata
      241 tttcatcaac atcaattaat tgttctattg aaatatcctg ttctatagca ctagtcagct
      301 gaataataac tttacgcatc acaccattca cacagcctga agataatggt gttgtataaa
      361 ccnnnnnnnn nnnnnntcag ccaaaacaca ttagatgccg ttgcttcaca aagcatgttt
      421 ttgctattta atatcagaca gtcatcaaat ttcatttctt gtgcatatat tgatgctaaa
      481 acataaagaa ggctattaca gtttttaaat ccggaaagtt tgttaattgg tttttgttgc
      541 tcactataaa ttcctaattc aataaaacac ttaggtattg tatattcaga ttctactgca
      601 gttattggaa gcatacatag tgaggcattg ttctgttcag gcaggtaagt gcctccggca
      661 tttcgcgtaa tcaacaatcg tagtttgctg ccatctgtaa aattatttct ttgattcatc
      721 tctaaaataa tttcacgtag ataatcttca gttataaaat cgcacttaag attcagaaca
      781 gcaacccctg caagtaagcg ttcaatatgt agtgacaaaa aatttgcttt acccctactg
      841 caacgaatgg attcaaaaag tccatcgcca taaataaatc ctctattgtt tagcttcaga
      901 acgggagttg cggcatcaac ccattgtcca tcataatacg cccacttcat gctgacagat
      961 ttttcttgct aattcaacaa tattgctgtt tgattcaatc agatgaccct ttactcctgc
     1021 tctgtttgca gcaatcatgt ctgtttccat atcaccgata aagtgtgcat tattcttatc
     1081 aaccttataa cgtgcaatgg ccttttctaa caacaggcta tcgggtttgc ggcaaaggca
     1141 tttactcttc tcaggat
//
LOCUS       DAORQT010000388         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_27716_length_1137_cov_7.031423, whole genome shotgun sequence.
ACCESSION   DAORQT010000388 DAORQT010000000
VERSION     DAORQT010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27716_length_1137_cov_7.031423"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1137
                     /locus_tag="PK134_09215"
     CDS             <1..>1137
                     /locus_tag="PK134_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRB39069.1"
                     /translation="VLPAKIPNLLVNGATGIAVGMATNMPPHNLTEVCNAVIAYIDNR
                     DITVEEMLQFVKAPDFPTGGIIYGYSGVKEALLTGRGRVVMRAKANIETLDNGRERII
                     VTEIPYMINKAEMIKKTADLVNDKKIEGITDIRDESGRDGMRIVYEIKRDAMASVVLN
                     NLYKHTPLQTSFGVNNIALVNGRPLLLNLKDLIHYYVEHRHDVLVRKTKFELAEAEKR
                     AHILEGLLIALDHLDEVIALIRASQTPDEARTGLIEKFALSEIQAKAILDMRLQRLTG
                     LERDKIKEEYAELMKLIDYLKKVLGSEELRMLLLKKEVAEILEKYGDERRTEIVYAAD
                     DISMEDMIEDEAVVVTISHMGYIKRTPLTEYRTQNRGGRGSIGSS"
BASE COUNT          368 a          211 c          255 g          303 t
ORIGIN      
        1 gtattacctg caaaaattcc aaatcttttg gttaatggtg ctacaggtat tgctgtgggt
       61 atggcaacca acatgcctcc acacaattta acagaggtgt gtaatgcagt aattgcctat
      121 attgacaaca gagatattac tgttgaagaa atgcttcagt ttgtaaaagc acctgatttt
      181 cctacaggag gaattattta cggatattcc ggtgttaaag aagctttgct tacaggcaga
      241 ggtcgtgtgg tgatgcgtgc aaaggcaaat attgaaacac ttgacaatgg acgcgaacgc
      301 attatcgtta cagaaattcc gtacatgatc aacaaggctg aaatgattaa gaaaacagcc
      361 gaccttgtta atgataaaaa aattgaaggc attactgaca tccgcgatga aagcggacgt
      421 gatggtatgc gcattgtgta tgagataaaa cgcgatgcaa tggccagcgt agtactgaac
      481 aatctttaca aacatacacc tctgcaaaca tcttttggcg taaacaatat tgcattggta
      541 aatggtcgtc cgttgttgct caacctcaaa gaccttatcc attactatgt agaacatcgc
      601 catgatgtgc tggtacgtaa aacaaaattt gaattagccg aagcagaaaa gcgtgctcac
      661 attttagaag gcttactcat tgctctcgat catttagacg aagttattgc attgattcgt
      721 gcttcgcaga cacctgatga agcccgcacc ggattgattg aaaaatttgc actctccgaa
      781 attcaggcaa aagctatttt ggacatgcgc ttacagcgtt tgacaggtct tgagcgcgac
      841 aagattaaag aagagtatgc agaattgatg aaacttattg actatctgaa aaaagtattg
      901 ggcagcgaag agttgcgtat gcttttattg aaaaaagaag ttgcagaaat tttagaaaaa
      961 tatggtgatg aacgcagaac agaaatagtt tacgctgcag acgacatcag catggaagac
     1021 atgattgaag acgaagctgt tgttgttacc atttctcaca tgggttacat caaacgcaca
     1081 ccgctgacag agtaccgcac acaaaaccgt ggtggtcgtg gttcaattgg cagcagc
//
LOCUS       DAORQT010000389         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_28471_length_1116_cov_3.620170, whole genome shotgun sequence.
ACCESSION   DAORQT010000389 DAORQT010000000
VERSION     DAORQT010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28471_length_1116_cov_3.620170"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PK134_09220"
     CDS             <1..867
                     /locus_tag="PK134_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB39070.1"
                     /translation="NPNILVMAIDENYLQTGGYTIATGRNMTQTEAMRGDNVVMIGSE
                     IKSKLFPGADAVDKEVYIDNRKYRIIGVLAEKGNSMGFGGDKVCLISLINGKQNYGSD
                     NRSWTVSVKVNHVSGLEAAVDEATGLFRTIRKDRVGEESSFEIIKSDSIAAIVIDQLK
                     NVSIAATLIGLITLLGAAIALMNIMLVSVTERTREIGIRKSLGATRLVILKQFLIEAI
                     VICLLGGGAGVILGVLVGNLMGMAMNAVFVVPWNWIIAGLLLCGFIGIASGFYPAMKA
                     SRLDPIEALRYE"
BASE COUNT          355 a          166 c          242 g          353 t
ORIGIN      
        1 aatccgaata ttttggttat ggctattgat gaaaactatt tacaaaccgg aggttataca
       61 attgcaacag gtcgtaatat gacgcagaca gaagctatgc gtggcgataa tgtagtgatg
      121 attggctctg aaataaagag taaacttttt cctggtgctg atgctgttga caaagaagtt
      181 tatatagaca acagaaaata tagaattatt ggcgtacttg ccgagaaagg taacagcatg
      241 ggttttggtg gtgataaagt atgtttgatt tcattaatca acggtaagca aaactatggc
      301 agcgataatc gttcatggac agttagtgtt aaggtgaatc atgtgtcagg ccttgaagca
      361 gcagttgatg aagcaacagg actattcaga acaataagaa aagatcgtgt tggcgaagaa
      421 agctcatttg aaataataaa aagcgacagc attgcagcaa ttgtaattga tcagttgaag
      481 aatgtaagta ttgcagcaac gctgattgga cttatcacat tgcttggtgc agcaattgca
      541 ttgatgaata ttatgcttgt ctctgtcaca gagcgaaccc gcgaaatagg aatcagaaaa
      601 tcacttggtg caacaaggct tgtaatctta aagcagtttt tgattgaagc aattgttatt
      661 tgcctcttgg ggggcggagc aggagttatt ttaggtgtgt tggtcggcaa cctgatgggc
      721 atggctatga atgctgtttt tgtggtgcct tggaactgga ttattgccgg attgttgcta
      781 tgtggtttta ttggcattgc ctcaggtttt tatcctgcca tgaaagcttc gcgcctcgac
      841 ccaattgagg ctttacgcta cgaataaaat atttgatcat tctaattcat ctgaattatt
      901 ttattatata gttgattttc aaatttttaa tatattttac ttaaagtaat ttattgtagc
      961 gattcgctta ggatgagtat cttaaaaaaa agtaaaaatt ctatttcaag aaattaataa
     1021 taacactatc tttgccgtcc gcttaaaaaa ggctattaaa ctgaaatata tataaacaaa
     1081 tgtcaaacca gtacgaaacc gtattcattc ttacac
//
LOCUS       DAORQT010000390         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_29184_length_1096_cov_3.742555, whole genome shotgun sequence.
ACCESSION   DAORQT010000390 DAORQT010000000
VERSION     DAORQT010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29184_length_1096_cov_3.742555"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="coaBC"
                     /locus_tag="PK134_09225"
     CDS             complement(<1..742)
                     /gene="coaBC"
                     /locus_tag="PK134_09225"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRB39071.1"
                     /translation="MLQGKKILLGVTGSIAAYKTPELVRQLVQQGATVKVIVTAAAKD
                     FVSPLVLSTVSKNETLQTFTSEDNSTWNNHVELGLWCDIFLVAPLSANTLAKFANGIC
                     DNLLTAVFLSCRNTVMVAPAMDVDMFKHPSTQKNLEIIAKNNIKIIGPESGELASGLT
                     GKGRMTEPEDIVQEVINYFNPSLPLRNKIAVVTTGPTQEALDPVRFIGNHSSGKMGVA
                     IANCLAAKGASVILISGPSVSLGNLNKSI"
     gene            complement(747..1085)
                     /locus_tag="PK134_09230"
     CDS             complement(747..1085)
                     /locus_tag="PK134_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRB39072.1"
                     /translation="MNSRNVAQTTVTRDVVDFEKKTENIYESLAIIAKRANHIAAETR
                     EELHDKLSEFASSTDTLDEVFENREQIEISKYYERLPKPTLVAIQDFLDDKIYFRSPA
                     KEENKDSAEN"
BASE COUNT          301 a          218 c          210 g          367 t
ORIGIN      
        1 ttattgactt attcaaattt cctaaactaa ctgacgggcc tgagataaga attactgatg
       61 cgcctttagc agctaaacag tttgcaatgg caacacccat tttccctgag gaatgatttc
      121 caataaaccg aaccggatcc aatgcttcct gtgttgggcc tgtggtgaca actgcaattt
      181 tgtttcttaa aggtaacgat ggattaaaat aattgattac ttcctgcaca atatcttcag
      241 gctctgtcat gcgaccttta cctgtaagtc cgctggctaa ttcgccactc tccggaccga
      301 taattttaat attgtttttg gcaataatct caaggttttt ttgtgttgaa ggatgtttaa
      361 acatatccac atccattgca ggtgctacca taactgtatt gcggcaagac agaaaaactg
      421 cagtcagtaa attgtcgcaa atgccattgg caaactttgc aagtgtgttg gctgatagtg
      481 gagctaccag aaaaatatcg caccataaac ctaattcaac atggttattc caggtagaat
      541 tatcttctga agtaaatgtt tgaagtgttt cgttttttga aacggtagaa agaacaagag
      601 gtgaaacaaa atctttagca gcagcagtaa caattacttt aactgttgct ccttgctgca
      661 ccaattgtcg cactaattca ggtgttttgt aagctgcaat gcttcctgta acacccagca
      721 ggattttttt tccctgcaac atgattttaa ttttctgcgc tgtctttgtt ttcttctttt
      781 gcaggactac ggaaatagat tttatcatct aaaaagtcct gaatggcaac caatgttggc
      841 tttggcagac gctcgtaata tttagagatt tcaatctgct cacggttttc aaaaacctca
      901 tccaaagtat ctgttgaaga ggcaaactcg cttagtttat cgtgtaattc ttcgcgggtt
      961 tcggcagcaa tatgattggc acgttttgca ataatggcaa gactttcata gatgttttca
     1021 gtcttctttt caaagtcaac cacatcgcgg gttactgttg tttgtgctac gtttctgcta
     1081 ttcattgttt taattc
//
LOCUS       DAORQT010000391         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_30280_length_1068_cov_3.489635, whole genome shotgun sequence.
ACCESSION   DAORQT010000391 DAORQT010000000
VERSION     DAORQT010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30280_length_1068_cov_3.489635"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK134_09235"
     CDS             <1..406
                     /locus_tag="PK134_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRB39073.1"
                     /translation="PIVQGAHVLIHLTVNKSTTIVVLEIKAKGTVHVQCHRCLEEFEM
                     PLDISKAIIVKTSGEDKEVDDDNIILADNAHDIHLAQHFYDVVSLAIPIKVTHPDNGC
                     NEETLRTLEKYMVKNENTNDPRWDILKNVSNN"
     gene            426..611
                     /gene="rpmF"
                     /locus_tag="PK134_09240"
     CDS             426..611
                     /gene="rpmF"
                     /locus_tag="PK134_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRB39074.1"
                     /translation="MPNPKHKHSKSRRDKRRTHYKAEAPTIAICPTTGETHLYHRAYW
                     HEGKMYYKGKVVLEKAV"
     gene            627..>1068
                     /locus_tag="PK134_09245"
     CDS             627..>1068
                     /locus_tag="PK134_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HRB39075.1"
                     /translation="MIRIGVDIMGGDYAPLETTLGAIAALNDLSPQEVHLVLIGDEKK
                     ITEIIEGQGVNPKSFTIVNATEVIEMSDSATRAFSQKPNSGMAIGFGLLKQNKIDAFA
                     SAGNSGAMLVGSMFSVKAINGIQRPAISTILPQEDGSTGILLDVG"
BASE COUNT          350 a          213 c          224 g          281 t
ORIGIN      
        1 ccctattgtt cagggagcac atgttcttat tcatcttacc gtaaacaaaa gcaccaccat
       61 tgtggttctg gaaattaaag ctaaaggcac cgtacacgta cagtgccatc gttgtcttga
      121 agagtttgag atgcctttgg atataagcaa ggctattatc gttaaaacct ctggcgaaga
      181 caaagaagtg gatgatgaca acatcatttt agccgacaat gcccacgata ttcatctggc
      241 acagcatttt tatgatgtag tttcattagc tatacccata aaagtgacac atcctgacaa
      301 tggctgcaat gaagaaaccc ttcgtacact tgaaaagtat atggtgaaga atgaaaacac
      361 aaacgacccg cgatgggata ttttaaaaaa tgtaagtaac aattgatatt aaataattaa
      421 acgtcatgcc aaatccaaaa cacaaacact ccaagtcgag aagagataag cgcagaacac
      481 attacaaggc agaagctcca acaattgcca tttgtcctac cacaggcgaa acacatttat
      541 atcatcgtgc ctactggcat gagggcaaaa tgtactacaa aggcaaggtg gttttagaaa
      601 aagctgttta atcgctgtat tcctttatga taaggatagg agttgatatt atgggtggag
      661 attatgctcc actcgaaact accttaggtg caattgcagc attaaatgac ctttcgcctc
      721 aggaagtaca cctggttcta ataggtgatg agaaaaaaat aacagagata attgaagggc
      781 agggagtcaa ccctaagtcg tttacaattg taaacgctac agaggtgatt gaaatgtctg
      841 acagtgcaac acgtgctttc tctcagaaac ccaactcagg tatggccatt ggttttggcc
      901 tgctaaaaca aaacaagatt gatgcctttg ccagtgccgg aaattccggt gctatgttgg
      961 ttggcagcat gttcagcgtt aaagccatta atggtattca gcgtcctgct atctcaacca
     1021 ttttaccaca ggaagacggc tcaacaggaa ttttacttga tgttggtg
//
LOCUS       DAORQT010000392         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_31253_length_1046_cov_5.571140, whole genome shotgun sequence.
ACCESSION   DAORQT010000392 DAORQT010000000
VERSION     DAORQT010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31253_length_1046_cov_5.571140"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..991)
                     /locus_tag="PK134_09250"
                     /pseudo
     CDS             complement(1..991)
                     /locus_tag="PK134_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
BASE COUNT          324 a          243 c          189 g          290 t
ORIGIN      
        1 caccaccata agctttacgt gtgatgacag ttattcttgg caccgttgcc tcactaaatg
       61 catacaacaa tttagctccg ttagtaatga tggcattcca ctcctgatcg gtaccgggca
      121 aaaatcccgg aacatcttca aacaccaaca atggaatatt aaaacaatcg caaaaacgaa
      181 caaagcgtgc tgccttagtt gatgaatgat tatctaaaac acctgccaga aacataggct
      241 gattggcaac tacgccaatg ctacgaccgg ctaaacgcgc aaagccaaca acaatatttt
      301 cggcaaagtc tttatgtact tcaaaaaaag aattttcgtc tgctacctgc gctactacct
      361 ctcgtatgtc gtaaggctga tttggatttt caggtacaat cgtattcaaa ccctcacgca
      421 cttcattagc ttctgaatat gcaactgatg gcggcatctc ctcgcaattt gacggaatat
      481 aactcagtaa agttttaaga tactgaatac attccatctc attggcacaa gaaaaatgtg
      541 tcactccgga ttttgttgaa tgggttgatg ctccacctaa ctcttctgca cttacttcct
      601 catgggtaac tgtttttaca acattaggtc cggtaacaaa catatatgac gtgttttgta
      661 ccatcataat aaaatctgtt attgcaggtg agtaaacagc cccacctgca caaggtccca
      721 taatggcact caattgagga ataactcctg atgccaatgt gttacgataa aaaatatctg
      781 catagcctcc caatgaaaca acaccttcct gaatgcgggc acctccacta tcatttaagc
      841 cgattacagg tgcaccattt tgcatggcca aatccataat acggcaaatt ttttctgcat
      901 gtgtctcact cagggagcca ccaaatacag taaagtcctg agaaaaaaca tacaccaacc
      961 taccatgaat tgttacggaa ccggtaacaa ttcatggtag gttggggtat gttttttcta
     1021 aggactttaa tgtatttggt gggtga
//
LOCUS       DAORQT010000393         1027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_32103_length_1027_cov_4.694444, whole genome shotgun sequence.
ACCESSION   DAORQT010000393 DAORQT010000000
VERSION     DAORQT010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32103_length_1027_cov_4.694444"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="PK134_09255"
     CDS             complement(<1..>1027)
                     /locus_tag="PK134_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRB39076.1"
                     /translation="MSHLVVYSQEDINQETRTRKNETKVGENVQVLKKSDATLLERLN
                     QSDAEYVLLGLPEDIGIRANGGRGGAYSAWRPALFSLLNVQSNCFGDGSNILVLGHIN
                     FDDLMLAADGINFKTDKGLSKARELVEQIDERVFEVVKAIIQSGKEAIIVGGGHNNAY
                     PNIKAAATGLQLSGVIKTENINCINCDAHSDFRVMEGRHSGNGFRYAYEQKFLSKYAI
                     VGLHESYNAGNVLQFMMERPENFSLSFFEDIFIRENISFTQAVDKAIEHVKDNFCGIE
                     IDMDTIQNIPSSAKTSSGISTIQARQYINRVASKLNACYLHIAEAAPVLSHIKTDNKT
                     GKLIGYLI"
BASE COUNT          310 a          233 c          162 g          322 t
ORIGIN      
        1 caataagata gccgattaac ttacctgttt tattgtcagt cttaatgtgt gaaagcacgg
       61 gagcagcttc ggcaatatgt agataacaag catttaactt tgaggctacg cgattaatgt
      121 actgccttgc ctgaatggta ctgatgccac ttgaagtttt agcacttgaa ggaatattct
      181 gaatggtatc catatcaatt tcaataccgc agaagttgtc cttaacatgt tcaatggctt
      241 tatcaactgc ctgagtaaat gaaatatttt ctctgataaa aatatcttca aaaaaagaca
      301 gtgaaaaatt ttccggtctc tccatcataa actgaagcac attacctgca ttatagcttt
      361 catgcaaacc gacaatggca tacttactga gaaacttttg ctcataagca tatctaaatc
      421 cattgccgct gtgacggcct tccatgactc tgaaatcgga atgtgcatca caattgatac
      481 agtttatatt ctcagtttta atgactccac tcagttgcag gcctgttgca gcagccttaa
      541 tattaggata tgcattgtta tgccctccac caactatgat ggcttctttg ccggattgaa
      601 ttatggcctt tacaacctca aaaactcttt catcaatctg ttcaacaagt tctcttgctt
      661 tgctaagtcc tttatcagtt ttaaaattaa tcccatcggc agcaagcatc agatcatcaa
      721 aattaatatg accaagaact aaaatattac taccatcgcc aaaacaatta ctctgcacat
      781 tcaacaagct gaacaatgca ggtctccatg cactatatgc gccaccacga cctccattag
      841 ctctgattcc aatatcttca ggcagaccca ataatacata ttcagcatca gattgattta
      901 atcgttctag taatgtagca tcactttttt tgagcacctg cacattctcg cccaccttag
      961 tttcattctt gcgggtgcgt gtttcctgat taatatcttc ctgactgtaa acaacaagat
     1021 gactcat
//
LOCUS       DAORQT010000394         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_5362
            NODE_32494_length_1019_cov_4.170124, whole genome shotgun sequence.
ACCESSION   DAORQT010000394 DAORQT010000000
VERSION     DAORQT010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867579
            Sequence Read Archive: SRR5722848
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:02:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,853
            CDSs (total)                      :: 1,839
            Genes (coding)                    :: 1,814
            CDSs (with protein)               :: 1,814
            Genes (RNA)                       :: 14
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32494_length_1019_cov_4.170124"
                     /isolate="AMR_MDS_5362"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>260)
                     /locus_tag="PK134_09260"
     CDS             complement(<1..>260)
                     /locus_tag="PK134_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB39077.1"
                     /translation="GPVLSIQTFRTVEEAVEKANNTPYGLSGGVWSDKGSKVFKVTSK
                     IKAGVLWANTYNKFDPTSPFGGFKESGMGREGGMHGLSAYLK"
     assembly_gap    261..270
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<271..>1019)
                     /locus_tag="PK134_09265"
     CDS             complement(<271..>1019)
                     /locus_tag="PK134_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB39078.1"
                     /translation="DYALPGMAAQPLGVAGQIIPWNFPILMAAWKIAPALACGNTVVL
                     KPAETTPLTALKLAEIFRDADLPPGVVNIVTGAGATGAAIVNHPGIDKVAFTGSTDVG
                     KIIQKALAGTTKKYTLELGGKAANIIFEDAAIDQAVEGIVNGIFFNQGHVCCAGSRLF
                     VQESVANEVVRKLKDRMETLIIGDPLDKNTDIGAINSREQLNKIKALVKVGVNEGGDC
                     YQSSCTLPSKGFWFKPTIFTGVAQSSRIVQE"
BASE COUNT          278 a          230 c          182 g          319 t
ORIGIN      
        1 attttaaata tgcactaagt ccatgcatgc caccttcgcg acccatgccg ctttctttaa
       61 atcctccaaa tggcgaagta ggatcaaact tattataagt gttggcccat aatacacctg
      121 cttttatttt tgaagtaact ttaaatacct tactgccttt atcagaccat acgcctccac
      181 ttaaaccata aggtgtgttg tttgcttttt caactgcctc ttctactgtt ctgaatgttt
      241 gaattgaaag aacaggccca nnnnnnnnnn cttcctgaac aattctactt gactgtgcaa
      301 caccggtaaa tattgtaggc ttaaaccaaa aaccttttga cggcaatgtg caagatgact
      361 gataacagtc accgccttcg ttaacaccta ctttaaccaa tgcttttatt ttattcaatt
      421 gctcgcgtga gttgatggca cctatatcgg tattcttatc taatggatcg ccaatgatga
      481 gtgtttccat acggtctttg agtttgcgca caacttcgtt ggcaacactt tcctgaacaa
      541 acaatcgtga gcctgcacaa caaacatgtc cctgattaaa aaatattccg ttaacaatgc
      601 cttcaacagc ctgatcaatg gcagcatctt caaaaatgat gttagctgcc ttgcctccaa
      661 gttcaagggt gtattttttt gttgttcctg ctaatgcttt ctgaataatt tttccaacat
      721 cagtagaacc ggtaaaagct actttatcaa taccgggatg attaacaata gctgctccag
      781 tggctcctgc tcctgtaaca atgttcacta caccgggagg aaggtcggca tctctgaaaa
      841 tttctgccaa ttttaatgct gtaagtggag ttgtttctgc aggttttagc acaacagtat
      901 ttccacaggc aagtgcaggt gcaattttcc atgctgccat taatatcgga aaattccatg
      961 ggataatttg tccggcaaca cctaaaggtt gtgctgccat tccgggtaat gcgtaatca
//
